LOCUS       DAZPBM010000001        75744 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPBM010000001 DAZPBM010000000
VERSION     DAZPBM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.001"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(45..1295)
                     /gene="icd"
                     /locus_tag="ACHUZQ_000001"
     CDS             complement(45..1295)
                     /gene="icd"
                     /locus_tag="ACHUZQ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1023821.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1467..2120
                     /gene="rluE"
                     /locus_tag="ACHUZQ_000002"
     CDS             1467..2120
                     /gene="rluE"
                     /locus_tag="ACHUZQ_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1023822.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2130..2591
                     /gene="nudJ"
                     /locus_tag="ACHUZQ_000003"
     CDS             2130..2591
                     /gene="nudJ"
                     /locus_tag="ACHUZQ_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1023823.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2645..3751
                     /gene="mnmA"
                     /locus_tag="ACHUZQ_000004"
                     /gene_synonym="trmU"
     CDS             2645..3751
                     /gene="mnmA"
                     /locus_tag="ACHUZQ_000004"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1023824.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3787..4428
                     /gene="hflD"
                     /locus_tag="ACHUZQ_000005"
     CDS             3787..4428
                     /gene="hflD"
                     /locus_tag="ACHUZQ_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1023825.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4432..5802
                     /gene="purB"
                     /locus_tag="ACHUZQ_000006"
     CDS             4432..5802
                     /gene="purB"
                     /locus_tag="ACHUZQ_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1023826.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5970..6641
                     /gene="phoP"
                     /locus_tag="ACHUZQ_000007"
     CDS             5970..6641
                     /gene="phoP"
                     /locus_tag="ACHUZQ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1023827.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6641..8101
                     /gene="phoQ"
                     /locus_tag="ACHUZQ_000008"
     CDS             6641..8101
                     /gene="phoQ"
                     /locus_tag="ACHUZQ_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1023828.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8177..9298
                     /gene="roxA"
                     /locus_tag="ACHUZQ_000009"
                     /gene_synonym="ycfD"
     CDS             8177..9298
                     /gene="roxA"
                     /locus_tag="ACHUZQ_000009"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1023829.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9347..10573)
                     /gene="pepT"
                     /locus_tag="ACHUZQ_000010"
     CDS             complement(9347..10573)
                     /gene="pepT"
                     /locus_tag="ACHUZQ_000010"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1023830.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10823..11959
                     /gene="potA"
                     /locus_tag="ACHUZQ_000011"
     CDS             10823..11959
                     /gene="potA"
                     /locus_tag="ACHUZQ_000011"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1023831.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11943..12800
                     /gene="potB"
                     /locus_tag="ACHUZQ_000012"
     CDS             11943..12800
                     /gene="potB"
                     /locus_tag="ACHUZQ_000012"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1023832.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12797..13591
                     /gene="potC"
                     /locus_tag="ACHUZQ_000013"
     CDS             12797..13591
                     /gene="potC"
                     /locus_tag="ACHUZQ_000013"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1023833.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13588..14634
                     /gene="potD"
                     /locus_tag="ACHUZQ_000014"
     CDS             13588..14634
                     /gene="potD"
                     /locus_tag="ACHUZQ_000014"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1023834.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14790..15611)
                     /gene="cobB"
                     /locus_tag="ACHUZQ_000015"
     CDS             complement(14790..15611)
                     /gene="cobB"
                     /locus_tag="ACHUZQ_000015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1023835.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15627..16538)
                     /gene="nagK"
                     /locus_tag="ACHUZQ_000016"
     CDS             complement(15627..16538)
                     /gene="nagK"
                     /locus_tag="ACHUZQ_000016"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1023836.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16567..17811)
                     /gene="lolE"
                     /locus_tag="ACHUZQ_000017"
     CDS             complement(16567..17811)
                     /gene="lolE"
                     /locus_tag="ACHUZQ_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1023837.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17811..18512)
                     /gene="lolD"
                     /locus_tag="ACHUZQ_000018"
     CDS             complement(17811..18512)
                     /gene="lolD"
                     /locus_tag="ACHUZQ_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1023838.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18505..19704)
                     /gene="lolC"
                     /locus_tag="ACHUZQ_000019"
     CDS             complement(18505..19704)
                     /gene="lolC"
                     /locus_tag="ACHUZQ_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1023839.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            19966..21039
                     /locus_tag="ACHUZQ_000020"
     CDS             19966..21039
                     /locus_tag="ACHUZQ_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1023840.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21167..24613
                     /gene="mfd"
                     /locus_tag="ACHUZQ_000021"
     CDS             21167..24613
                     /gene="mfd"
                     /locus_tag="ACHUZQ_000021"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1023841.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24757..25713
                     /gene="ldtC"
                     /locus_tag="ACHUZQ_000022"
                     /pseudo
     CDS             24757..25713
                     /gene="ldtC"
                     /locus_tag="ACHUZQ_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25796..26053)
                     /gene="bhsA"
                     /locus_tag="ACHUZQ_000023"
                     /gene_synonym="ycfR"
     CDS             complement(25796..26053)
                     /gene="bhsA"
                     /locus_tag="ACHUZQ_000023"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR1023842.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26294..26926
                     /gene="comR"
                     /locus_tag="ACHUZQ_000024"
     CDS             26294..26926
                     /gene="comR"
                     /locus_tag="ACHUZQ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1023843.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(26988..27527)
                     /locus_tag="ACHUZQ_000025"
     CDS             complement(26988..27527)
                     /locus_tag="ACHUZQ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1023844.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(27754..29058)
                     /gene="ndh"
                     /locus_tag="ACHUZQ_000026"
     CDS             complement(27754..29058)
                     /gene="ndh"
                     /locus_tag="ACHUZQ_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1023845.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(29466..30008)
                     /gene="ycfP"
                     /locus_tag="ACHUZQ_000027"
     CDS             complement(29466..30008)
                     /gene="ycfP"
                     /locus_tag="ACHUZQ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1023846.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(30031..31056)
                     /gene="nagZ"
                     /locus_tag="ACHUZQ_000028"
     CDS             complement(30031..31056)
                     /gene="nagZ"
                     /locus_tag="ACHUZQ_000028"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1023847.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(31067..31891)
                     /gene="thiK"
                     /locus_tag="ACHUZQ_000029"
     CDS             complement(31067..31891)
                     /gene="thiK"
                     /locus_tag="ACHUZQ_000029"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1023848.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(31872..32513)
                     /gene="lpoB"
                     /locus_tag="ACHUZQ_000030"
     CDS             complement(31872..32513)
                     /gene="lpoB"
                     /locus_tag="ACHUZQ_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1023849.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(32527..32904)
                     /locus_tag="ACHUZQ_000031"
     CDS             complement(32527..32904)
                     /locus_tag="ACHUZQ_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1023850.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(32907..33266)
                     /gene="hinT"
                     /locus_tag="ACHUZQ_000032"
     CDS             complement(32907..33266)
                     /gene="hinT"
                     /locus_tag="ACHUZQ_000032"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1023851.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            33600..35789
                     /gene="fhuE"
                     /locus_tag="ACHUZQ_000033"
     CDS             33600..35789
                     /gene="fhuE"
                     /locus_tag="ACHUZQ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR1023852.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(35849..37282)
                     /gene="ptsG"
                     /locus_tag="ACHUZQ_000034"
     CDS             complement(35849..37282)
                     /gene="ptsG"
                     /locus_tag="ACHUZQ_000034"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1023853.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(37577..38374)
                     /locus_tag="ACHUZQ_000035"
     CDS             complement(37577..38374)
                     /locus_tag="ACHUZQ_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1023854.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(38385..39389)
                     /gene="holB"
                     /locus_tag="ACHUZQ_000036"
     CDS             complement(38385..39389)
                     /gene="holB"
                     /locus_tag="ACHUZQ_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1023855.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(39386..40027)
                     /gene="tmk"
                     /locus_tag="ACHUZQ_000037"
     CDS             complement(39386..40027)
                     /gene="tmk"
                     /locus_tag="ACHUZQ_000037"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1023856.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(40017..41039)
                     /gene="yceG"
                     /locus_tag="ACHUZQ_000038"
     CDS             complement(40017..41039)
                     /gene="yceG"
                     /locus_tag="ACHUZQ_000038"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1023857.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(41042..41851)
                     /gene="pabC"
                     /locus_tag="ACHUZQ_000039"
     CDS             complement(41042..41851)
                     /gene="pabC"
                     /locus_tag="ACHUZQ_000039"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1023858.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(41971..43212)
                     /gene="fabF"
                     /locus_tag="ACHUZQ_000040"
     CDS             complement(41971..43212)
                     /gene="fabF"
                     /locus_tag="ACHUZQ_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1023859.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(43300..43536)
                     /gene="acpP"
                     /locus_tag="ACHUZQ_000041"
     CDS             complement(43300..43536)
                     /gene="acpP"
                     /locus_tag="ACHUZQ_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1023860.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(43747..44481)
                     /gene="fabG"
                     /locus_tag="ACHUZQ_000042"
     CDS             complement(43747..44481)
                     /gene="fabG"
                     /locus_tag="ACHUZQ_000042"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1023861.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(44494..45423)
                     /gene="fabD"
                     /locus_tag="ACHUZQ_000043"
     CDS             complement(44494..45423)
                     /gene="fabD"
                     /locus_tag="ACHUZQ_000043"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1023862.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(45439..46392)
                     /gene="fabH"
                     /locus_tag="ACHUZQ_000044"
     CDS             complement(45439..46392)
                     /gene="fabH"
                     /locus_tag="ACHUZQ_000044"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1023863.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(46460..47530)
                     /gene="plsX"
                     /locus_tag="ACHUZQ_000045"
     CDS             complement(46460..47530)
                     /gene="plsX"
                     /locus_tag="ACHUZQ_000045"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1023864.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(47611..47784)
                     /gene="rpmF"
                     /locus_tag="ACHUZQ_000046"
     CDS             complement(47611..47784)
                     /gene="rpmF"
                     /locus_tag="ACHUZQ_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1023865.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(47836..48357)
                     /gene="yceD"
                     /locus_tag="ACHUZQ_000047"
     CDS             complement(47836..48357)
                     /gene="yceD"
                     /locus_tag="ACHUZQ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1023866.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            48556..49140
                     /gene="yceF"
                     /locus_tag="ACHUZQ_000048"
     CDS             48556..49140
                     /gene="yceF"
                     /locus_tag="ACHUZQ_000048"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1023867.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(49252..50211)
                     /gene="rluC"
                     /locus_tag="ACHUZQ_000049"
     CDS             complement(49252..50211)
                     /gene="rluC"
                     /locus_tag="ACHUZQ_000049"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1023868.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            50784..53969
                     /gene="rne"
                     /locus_tag="ACHUZQ_000050"
     CDS             50784..53969
                     /gene="rne"
                     /locus_tag="ACHUZQ_000050"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1023869.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(54164..55117)
                     /gene="flgL"
                     /locus_tag="ACHUZQ_000051"
     CDS             complement(54164..55117)
                     /gene="flgL"
                     /locus_tag="ACHUZQ_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR1023870.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(55129..56772)
                     /gene="flgK"
                     /locus_tag="ACHUZQ_000052"
     CDS             complement(55129..56772)
                     /gene="flgK"
                     /locus_tag="ACHUZQ_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR1023871.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(56838..57779)
                     /gene="flgJ"
                     /locus_tag="ACHUZQ_000053"
     CDS             complement(56838..57779)
                     /gene="flgJ"
                     /locus_tag="ACHUZQ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1023872.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(57779..58876)
                     /gene="flgI"
                     /locus_tag="ACHUZQ_000054"
     CDS             complement(57779..58876)
                     /gene="flgI"
                     /locus_tag="ACHUZQ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1023873.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(58888..59586)
                     /gene="flgH"
                     /locus_tag="ACHUZQ_000055"
     CDS             complement(58888..59586)
                     /gene="flgH"
                     /locus_tag="ACHUZQ_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1023874.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(59639..60411)
                     /gene="flgG"
                     /locus_tag="ACHUZQ_000056"
                     /pseudo
     CDS             complement(59639..60411)
                     /gene="flgG"
                     /locus_tag="ACHUZQ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(60583..61338)
                     /gene="flgF"
                     /locus_tag="ACHUZQ_000057"
     CDS             complement(60583..61338)
                     /gene="flgF"
                     /locus_tag="ACHUZQ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR1023875.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(61358..62563)
                     /gene="flgE"
                     /locus_tag="ACHUZQ_000058"
     CDS             complement(61358..62563)
                     /gene="flgE"
                     /locus_tag="ACHUZQ_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1023876.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(62588..63283)
                     /gene="flgD"
                     /locus_tag="ACHUZQ_000059"
     CDS             complement(62588..63283)
                     /gene="flgD"
                     /locus_tag="ACHUZQ_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1023877.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(63295..63699)
                     /gene="flgC"
                     /locus_tag="ACHUZQ_000060"
     CDS             complement(63295..63699)
                     /gene="flgC"
                     /locus_tag="ACHUZQ_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR1023878.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(63703..64119)
                     /gene="flgB"
                     /locus_tag="ACHUZQ_000061"
     CDS             complement(63703..64119)
                     /gene="flgB"
                     /locus_tag="ACHUZQ_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1023879.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            64274..64933
                     /gene="flgA"
                     /locus_tag="ACHUZQ_000062"
     CDS             64274..64933
                     /gene="flgA"
                     /locus_tag="ACHUZQ_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1023880.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            65009..65302
                     /gene="flgM"
                     /locus_tag="ACHUZQ_000063"
     CDS             65009..65302
                     /gene="flgM"
                     /locus_tag="ACHUZQ_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1023881.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            65307..65723
                     /gene="flgN"
                     /locus_tag="ACHUZQ_000064"
     CDS             65307..65723
                     /gene="flgN"
                     /locus_tag="ACHUZQ_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1023882.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(65763..67298)
                     /gene="murJ"
                     /locus_tag="ACHUZQ_000065"
     CDS             complement(65763..67298)
                     /gene="murJ"
                     /locus_tag="ACHUZQ_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1023883.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSLAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(67408..68331)
                     /locus_tag="ACHUZQ_000066"
     CDS             complement(67408..68331)
                     /locus_tag="ACHUZQ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1023884.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(68333..68980)
                     /locus_tag="ACHUZQ_000067"
     CDS             complement(68333..68980)
                     /locus_tag="ACHUZQ_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR1023885.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(68991..69575)
                     /gene="rimJ"
                     /locus_tag="ACHUZQ_000068"
     CDS             complement(68991..69575)
                     /gene="rimJ"
                     /locus_tag="ACHUZQ_000068"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1023886.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            69811..71019
                     /gene="mdtH"
                     /locus_tag="ACHUZQ_000069"
     CDS             69811..71019
                     /gene="mdtH"
                     /locus_tag="ACHUZQ_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1023887.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            71083..71730
                     /gene="grxB"
                     /locus_tag="ACHUZQ_000070"
     CDS             71083..71730
                     /gene="grxB"
                     /locus_tag="ACHUZQ_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1023888.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            71864..72424
                     /locus_tag="ACHUZQ_000071"
     CDS             71864..72424
                     /locus_tag="ACHUZQ_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1023889.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            72530..73576
                     /gene="pyrC"
                     /locus_tag="ACHUZQ_000072"
     CDS             72530..73576
                     /gene="pyrC"
                     /locus_tag="ACHUZQ_000072"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1023890.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            73650..73895
                     /gene="dinI"
                     /locus_tag="ACHUZQ_000073"
     CDS             73650..73895
                     /gene="dinI"
                     /locus_tag="ACHUZQ_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1023891.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            74185..74439
                     /gene="bssS"
                     /locus_tag="ACHUZQ_000074"
     CDS             74185..74439
                     /gene="bssS"
                     /locus_tag="ACHUZQ_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1023892.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            74554..75672
                     /gene="solA"
                     /locus_tag="ACHUZQ_000075"
     CDS             74554..75672
                     /gene="solA"
                     /locus_tag="ACHUZQ_000075"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1023893.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        18397 a        20159 c        18958 g        18230 t
ORIGIN      
        1 cgggcgtgtt atacgcccgt ttcaatattt aactcatgga gagattacat gttttcgatg
       61 atcgcttcac caaactctga acatttcagc agcttagcgc cttccatcag acgttcaaag
      121 tcataagtta cggttttcgc gttgatagca ccttccatac ctttaacaat taaatcagcc
      181 gcttcagtcc accccatatg gcgcagcatc atctcagcgg agaggatgat agagcctgga
      241 tttactttgt cctgaccggc atatttcggc gcagtaccgt gggtggcttc aaacagggcg
      301 cattcgtcac cgatgtttgc gccaggggcg ataccgatac cgccaacctg cgctgccagg
      361 gcgtcagaaa tgtagtcacc gttcaggttc atacaggcga taacatcata ttcagccgga
      421 cgcagcagga tctgttgcag gaatgcatca gcaatcacgt ctttaatgac gatctctttg
      481 ccggtgttcg ggtttttaac tttcagccac gggccgccgt cgatcagttc accgccaaac
      541 tcttcacgcg ccagctggta gccccagtct ttaaacgcgc cttcggtgaa cttcatgatg
      601 ttgcctttgt gcaccagagt cacagagtca cgatcgttag caattgcgta ttcgatcgct
      661 gcacgaacca gacgtttggt gccttcttcc gaacacggct taataccgat accacaatgt
      721 tccgggaagc gaattttctt cacccccatc tcttcacgca ggaatttaat cactttctcg
      781 gcgtcggcag agtctgcttt ccattcgata cccgcataaa tgtcttccga gttttcacgg
      841 aagataacca tatcggtcag ttcagggtgt ttaaccgggc ttggagtgcc ctgatagtaa
      901 cgtaccggac gcaggcagat gtagagatcc agttcctggc gcagggcaac gttcagagag
      961 cgaataccgc caccaacagg agtggtcagc ggacctttaa tggcaacgcg atattcacga
     1021 atcagatcaa gggtttcagc aggcagccag acatcctgac cataaacctg tgtggatttt
     1081 tcaccggtgt aaatttccat ccaggagatt ttacgctcgc ctttataggc tttctcgact
     1141 gcagcgtcga ccactttcag catggctggg gttacatcta caccgattcc atcaccttca
     1201 atgtaaggga taatcggatt ttcaggaacg ttgagtttgc cgttttgcag ggtgatcttc
     1261 ttgccttgtg ccggaacaac tactttactt tccattcacc tctccttcga gcgctactgg
     1321 tttgctcgtc tgccaccacg ttgcatatgc gtttgcgtcc tgcgatacgg atgctttaga
     1381 gcaatttttt gttaatgatt tgtaattggc ttgtcaatgc taccctattg tttgccgtca
     1441 tgaaagatgc gcgttattag gctataatgc ggcaattcat aatctctgaa aataccatgc
     1501 aaaaaacttc ttttagaaat caccaggtta agcgattcag ctcgcaacgt tctactaggc
     1561 gtaaacctga aaaccagccc acgcgtgtga tcctgttcaa taaaccctac gatgttcttc
     1621 cgcagttcac cgatgaagcc ggacgcaaaa cattaaaaga attcatcccg gttcagggcg
     1681 tttatgcagc aggtcgtctt gaccgcgata gcgaagggtt actggtgctg accaataacg
     1741 gcgcgttgca ggcgcgttta acccagccgg gcaaacgcac cgggaaaatc tattatgtgc
     1801 aggtggaagg tattcccaca caagacgcac ttgaagcctt gcgcaatggc gtaaccttaa
     1861 atgatggccc taccctgccc gccggcgcgg agctggttga cgaaccagcg tggttatggc
     1921 cgcggaatcc accaattcgt gaacgcaaaa gtattcccac cagctggctg aagatcacct
     1981 tatatgaagg acgtaatcgc caggtgcgcc gcatgaccgc ccatgttggt ttccccacgc
     2041 tgcgactgat tcgttatgcg atgggtgatt acactttgga taaccttgcc aatggcgaat
     2101 ggcgagaagc aacagaataa ggatgcacaa tgtttaaacc gcacgttacc gttgcttgcg
     2161 tagtgcacgc agagggtaaa tttttagtcg ttgaagagac gattaatggt aaagcgttat
     2221 ggaaccaacc tgccgggcat ctggaagccg atgaaacctt agtggaagct gccgcccgtg
     2281 agctatggga agaaaccggc atcagcgcgc aaccgcaaca ctttattcgt atgcatcagt
     2341 ggattgcgcc agataaaacg ccgtttttgc gtttcctctt tgccattgag cttgagcaaa
     2401 tatgcccgac ccagcctcat gacagcgata tcgactgctg tcgttgggtc agcgccgaag
     2461 aaattttaaa ggcgtcaaat cttcgttcgc cgctggtggc ggaaagtatc cgttgttatc
     2521 aaagtgggca acgctatccg ctggagatga ttggcgattt taactggcct tttacaaagg
     2581 gtgtcatcta aaaggctgcg tgatagaata cgccgccttg aagttcaatg tcgtgagtga
     2641 tccaatgtct gaaaccgcaa aaaaagtaat cgtcggcatg tccggcggtg tcgattcctc
     2701 cgtttctgcc tggctgttgc aacaacaggg atatcaggtt gaaggcctgt ttatgaagaa
     2761 ctgggaagaa gacgacggtg aggaatattg cacagcggca gcggatctgg ctgatgccca
     2821 ggctgtctgc gacaagctcg gcattgaact gcacaccgtt aactttgctg ccgagtactg
     2881 ggacaatgtc ttcgaactgt tccttgccga atataaagcc ggtcgcacgc cgaatccgga
     2941 tattctgtgc aacaaagaga tcaaatttaa agctttcctc gaatttgccg ccgaagattt
     3001 aggtgccgat tatatcgcta ccggtcatta cgtacgtcgg gccgatgtcg atggcaagag
     3061 ccgcctgctg cgtggtctgg acagcaataa agaccagagc tacttccttt atacgctcag
     3121 ccatgagcag attgcgcaaa gcctgttccc ggtcggcgaa ctggaaaaac cgcaggtgcg
     3181 taagattgct gaagatcttg gtctggtcac cgcgaagaaa aaagactcta ccggcatttg
     3241 cttcattggc gaacgtaaat tccgcgagtt cctgggccgt tatctcccgg ggcaaccggg
     3301 caaaatcatt accgtcgatg gcgatgaaat tggtgagcac caggggctga tgtatcacac
     3361 tctcggtcag cgtaaaggtc tgggtatcgg tggcaccaaa gaaggcaccg aagaaccttg
     3421 gtatgtggtg gacaaagacg tcgaaaacaa cattctggtt gtcgctcagg gccatgaaca
     3481 cccgcggctg atgtctgtcg ggttgattgc ccagcagttg cactgggtcg atcgcgaacc
     3541 attcaccggc actatgcgtt gcacggtaaa aacccgctat cgccagaccg atatcccttg
     3601 caccgtcaag gcgctggacg atgatcgcat tgaagtgatt ttcgatgaac cggttgccgc
     3661 cgtgacgccg ggccagtctg ccgtcttcta taacggtgaa gtgtgcctcg gtggcggtat
     3721 tattgagcag cgtctgccgc tgccggtctg attattatct ttacttaaaa caaggaagca
     3781 gtgaacgtgg caaagaatta ctatgacatc accctcgccc tggccggtat ttgtcagtcg
     3841 gcacgcctgg tgcaacaact cgctcaccag gggcattgtg atgccgatgc gctacacgtc
     3901 tcactcaaca gtattattga tatgaacccc agctcgacgc tggcggtttt tggcggtagc
     3961 gaagccaacc tgcgcgtcgg gctggaaacc ctgctcggcg tgctcaatgc cagcagtcgc
     4021 cagggcttaa acgccgaatt aacccgctac acactcagct tgatggtgct tgagcgcaaa
     4081 ctctcctcag cgaaaggcgc gctcgacact ctgggcaacc ggatcaacgg cctgcaacgc
     4141 cagctcgaac acttcgattt acagtccgaa acgctgatga gcgcgatggc tgctatctat
     4201 gttgatgtga tcagcccgct tggcccacgc attcaggtca ccggttcccc tgctgtactg
     4261 caaagcccac aagtgcaggc gaaagttcgc gcaaccctgc tggcaggcat tcgcgccgcc
     4321 gtgctctggc accaggtcgg cggcggacgt ctgcaactga tgttttctcg taatcgcctg
     4381 accactcagg caaaacaaat tcttgctcat ttaaccccgg agttgtgatc tatggaatta
     4441 tcctcactga ccgccgtttc ccctgtcgat ggacgctacg gcgataaagt cagcgcgctg
     4501 cgcgggattt tcagcgaata tggtttgctg aaattccgtg tacaagttga agtacgttgg
     4561 ctgcaaaaac tggccgcgca cgcagcgatc aaggaagttc ctgcttttgc tgccgacgca
     4621 atcggttacc ttgatgcaat tgtcgccaat ttcagcgaag aagatgccgc acgcatcaaa
     4681 accatcgagc gtaccactaa ccacgacgtt aaagcggttg agtatttcct gaaagaaaaa
     4741 gtggcggaga tcccggaact gcacgcggtt tctgaattca tccactttgc ctgtacttcg
     4801 gaagatatca ataacctctc ccacgcatta atgctgaaaa ccgcgcgtga tgaagtgatc
     4861 ctgccgtact ggcgtcaact gattgatggc attaaagatc tcgccgttca gtaccgcgat
     4921 atcccgctgc tgtctcgtac ccacggtcag ccagccacgc cgtcaaccat cggtaaagag
     4981 atggcaaacg tcgcctaccg tatggagcgc cagtaccgcc agcttaacca ggtggagatc
     5041 ctcggcaaaa tcaacggcgc ggtcggtaac tataacgccc acatcgccgc ttacccggaa
     5101 gttgactggc atcagttcag cgaagagttc gtcacctcgc tgggtattca atggaacccg
     5161 tacactaccc agattgaacc gcacgactac atcgccgagc tgtttgattg cgttgcacgc
     5221 ttcaacacca ttctgatcga ctttgatcgt gacgtctggg gttatatcgc ccttaaccac
     5281 ttcaaacaga aaaccattgc tggtgagatt ggttcttcca ccatgccgca taaagttaac
     5341 ccgatcgact tcgagaactc cgaagggaat ctgggccttt ccaacgcggt attacagcat
     5401 ctggcaagca aactgccggt ttcccgctgg cagcgtgacc tgaccgactc caccgtgctg
     5461 cgtaacctcg gcgtgggtat cggttatgcg ctgattgcgt atcaatccac cctgaaaggc
     5521 gtgagcaaac tggaagtgaa ccgtgaccat ctgctggatg agctggatca caactgggaa
     5581 gtgctggctg agccaatcca gacagttatg cgtcgctatg gcatcgaaaa accgtacgag
     5641 aagctgaaag agctgactcg tggtaagcgc gttgacgccg aaggcatgaa gcagtttatc
     5701 gacggtctgg cgttgccaga agaagagaaa gcccgcctga aagcgatgac gccggcaaac
     5761 tacattggtc gcgccatcac catggttgat gagctgaaat aaactcgtat cagtgccgga
     5821 tggcgatgct gtccggcctg cttattaaga ttatccgctt tttatttttt cactttacct
     5881 cccctccccg ctggtttatt taatgtttac ccccataacc acataatcgc gttacactat
     5941 tttaataatt aagacaggga gaaataaaaa tgcgcgtact ggttgttgaa gacaatgcgt
     6001 tgttacgtca ccaccttaaa gttcagattc aggatgctgg tcatcaggtc gatgatgcag
     6061 aagatgccaa agaagccgat tattatctca atgaacattt accggatatt gcgattgtcg
     6121 atctcggatt gccagacgag gacggtctgt cactgattcg ccgctggcgt agcaatgatg
     6181 tttcactgcc gattctggta ttaaccgccc gtgaaagctg gcaggacaaa gtcgaagtat
     6241 taagtgctgg tgctgatgat tacgtgacta aaccatttca tattgaagag gtgatggcgc
     6301 gaatgcaggc attaatgcgg cgtaatagcg gtctggcttc acaggtcatt tcgctaccac
     6361 cgtttcaggt tgatctctct cgccgtgaat tatctattaa tgacgaagtg atcaaactga
     6421 ccgcgttcga atacaccatt atggaaacgt tgatacgcaa taatggcaaa gtggtgagca
     6481 aagattcgtt aatgctccaa ctctatccgg atgcggagct gcgggaaagc cataccattg
     6541 atgtactgat gggacgtctg cgcaaaaaaa ttcaggcaca atatccgcaa gaagtgatta
     6601 ctaccgttcg cggccagggc tatttgttcg aattgcgctg atgaaaaaat tactgcgtct
     6661 ttttttcccg ctctcgctgc gggtacgttt tctgttggca acggctgcgg tagtactggt
     6721 gctttcgctt gcctacggaa tggtcgcgct gatcggttat agcgtcagtt tcgataaaac
     6781 tacgtttcgg ctgttacgtg gcgagagcaa tctgttctat acccttgcga agtgggaaaa
     6841 caataagttg catgtcgagt tacccgaaaa tatcgacaag caaagcccca ccatgacgct
     6901 aatttatgat gagaacgggc agcttttatg ggcgcaacgt gacgtgccct ggctgatgaa
     6961 gatgatccag cctgactggc tgaaatcgaa tggttttcat gaaattgaag cggatgttaa
     7021 cgataccagc ctcttgctga gtggagatca ttcgatacag caacagttgc aggaagtgcg
     7081 ggaagatgat gacgacgcgg agatgaccca ctcggtggca gtaaacgtct acccggcaac
     7141 atcgcggatg ccaaaattaa ccattgtggt ggtggatacc attccggtgg agctaaaaag
     7201 ttcctatatg gtctggagct ggtttatcta tgtgctctca gccaatctgc tgttagtgat
     7261 cccgctgctg tgggtcgccg cctggtggag tttacgcccc atcgaagccc tggcaaaaga
     7321 agtccgcgaa ctggaagaac ataaccgcga attgctcaat ccagccacaa cgcgagaact
     7381 gaccagtctg gtacgaaacc tgaaccgatt gttaaaaagt gaacgcgaac gttacgacaa
     7441 ataccgtacg acgctcaccg acctgaccca tagtctgaaa acgccactgg cggtgctgca
     7501 aagtacgctg cgttctctgc gtagtgaaaa gatgagcgtc agtgatgctg agccggtaat
     7561 gctggagcaa atcagccgca tttcacagca aattggctac tacctgcatc gtgccagtat
     7621 gcgcggcggg acattgctca gccgcgagct gcatccggtc gccccactgc tggacaatct
     7681 cacctcagcg ctgaacaaag tgtatcaacg caaaggggtc aatatctctc tcgatatttc
     7741 gccagagatc agctttgtcg gtgagcagaa cgattttgtc gaggtgatgg gcaacgtgct
     7801 ggataatgcc tgtaaatatt gcctcgagtt tgtcgaaatt tctgcaaggc aaaccgacga
     7861 gcatctctat attgtggtcg aggatgatgg ccccggtatt ccattaagca agcgagaggt
     7921 cattttcgac cgtggtcaac gggttgatac tttacgccct gggcaaggtg tagggctggc
     7981 ggtagcccgc gaaatcaccg agcaatatga gggtaaaatc gtcgccggag agagcatgct
     8041 gggcggtgcg cggatggagg tgatttttgg tcgccagcat tctgcgccga aagatgaata
     8101 aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg cagataccag
     8161 cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt ttcttgaacg
     8221 tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta ttgacccgat
     8281 ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc gactggtcag
     8341 tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg atcatctcgg
     8401 tgaaaccaac tggtcattac tggtacaggc agtgaaccac tggcatgagc cgaccgccgc
     8461 gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga tgatttcttt
     8521 ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt ttatcattca
     8581 gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac agcactgccc
     8641 acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag agctggagcc
     8701 tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc tggaaaatgc
     8761 gatgaactat tccgtgggct ttcgcgcgcc aaatacgcgg gaactgatta gtggatttgc
     8821 cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatcctg atgttccacc
     8881 tcgcgctcat cctgcggatg ttctgcctca agagatggat aaactgcgtg agatgatgct
     8941 cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta tatcccagtc
     9001 acgtcatgaa ctggatatcg cgccgccaga accgccttat cagccagatg aaatctacga
     9061 tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat tgcgcattgg
     9121 cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg cactggatgc
     9181 actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg aagatccgtc
     9241 attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg aagggtaagt
     9301 ttggatttaa gccggatgcg gcatcacatg ccgcatccct tttcgcttac ttccgttgcg
     9361 ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct tccagagtca
     9421 caaactcatg cttaccatga tagttgtaac cgccagtgaa caagttcggg cacggtaatc
     9481 ccataaacga caactgcgcg ccgtcggtac caccgcggat cggtttcagt tccggttcaa
     9541 tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc tcaaccactt
     9601 tctctcgcat attgtagtaa ctgtcttcaa tcaccagttc aatgtagcaa tcaggatgta
     9661 accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct tcaaactgtt
     9721 tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg cctttcatgc
     9781 tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca tccgccggaa
     9841 cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct ttcgccgtgc
     9901 ccggatgaac attgttaccg acaattttga tattgaccga tgcggcgttg aagttttcaa
     9961 actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca tcgaaggcat
    10021 caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag gcgacgcgaa
    10081 tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg atttctgcaa
    10141 tacctgcttt gtcatcggca cctaacaagg ttttaccatc ggtggtaatc aacgtctgac
    10201 ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg ccgataccca
    10261 gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt ttgccgctgc
    10321 aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg ccagggacgt
    10381 tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg ataagcccca
    10441 tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc gtgctgggaa
    10501 cctgtctcac ccctgctttt gattgggtat ccagagatac gtagttcaaa aatcgctcaa
    10561 gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga agcaagaaaa
    10621 gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat cagcattacc
    10681 ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc aagcggtttg
    10741 ccgtagaatt accgccctca ttaagagtca ccaaggtggt taaccacaaa ccccgcatcg
    10801 gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac aaccgagttc
    10861 gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta aagaggtcat
    10921 tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg gcccttctgg
    10981 ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg attccggacg
    11041 catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct atgtgaacac
    11101 tgttttccaa agctacgcac ttttccccca catgaccgtg ttcgaaaatg tggcctttgg
    11161 gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg aagccctgcg
    11221 gatggtgcaa ctggaaacct tcgctcaacg taaaccgcat cagctctctg gtggtcaaca
    11281 gcaacgcgtc gccattgctc gcgcggtggt taacaagcct cgtctgttgt tgctggatga
    11341 gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc tgaaagcgtt
    11401 acagcgtaag cttggtatta ccttcgtctt tgtcactcac gatcaggaag aagcgctcac
    11461 catgtcagac aggattgtgg tgatgcgcga gggtcgtatt gagcaagatg gtacaccgcg
    11521 cgaaatttac gaagagccga aaaacctgtt tgttgccggc ttcattggcg aaatcaatat
    11581 gtttaacgcc actgtcatcg aacgtctcga cgagcagcgc gtacgcgcca acgttgaagg
    11641 ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac tgcatgttct
    11701 gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg ctgaagggct
    11761 gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg ttgttgaact
    11821 ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgacc ctgactttga
    11881 ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg tcgtactggc
    11941 tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc ggttggcttg
    12001 tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg acccgcgacg
    12061 acgccagttt cgtcaaaatg gtctttacgc tggataacta cacgcgtctg ctcgatccgc
    12121 tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc ctcgcctgcc
    12181 tggtgctggg ctacccgttt gcctggtttc tggcgaagct gccacacaag gtgcgtccgc
    12241 tgctgctgtt tctgctgatt gttcccttct ggaccaactc actgattcgc atctacgggc
    12301 tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg ctgggcgtta
    12361 tcgacacacc aattcgtatc atgttcacgc ccagtgcggt gattatcggt ctggtttaca
    12421 ttctgctacc gtttatggtg atgccgctgt actccagtat cgaaaaactg gataagccgt
    12481 tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc cgtatcatta
    12541 ttccactgac gatgccggga attattgccg gatgtctgct ggtgatgctg ccagcgatgg
    12601 gcctgttcta tgtatccgac ctgatgggcg gtgcgaaaaa cctgctgatc ggtaacgtca
    12661 tcaaggtcca gttcctcaat attcgtgact ggccgtttgg tgcagccacc agcattacgc
    12721 tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt ctgctgaata
    12781 aaaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga ccgctatcta
    12841 cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca gctcgcgctt
    12901 tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga acaacgacag
    12961 cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga catttgctac
    13021 gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgtg gtaagccgtt
    13081 cgttagcgga atgctgtttg tggtgatgat gtcaccagat atcgtgatgg cgatttctct
    13141 gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc tgttctcgca
    13201 tatcaccttc tgcctgccgt ttgtggtggt gacggtgtat tcgcgcctga aaggttttga
    13261 cgtgcggatg ctggaagcgg cgaaagatct cggtgccagt gaatttacca ttctgcgaaa
    13321 aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa gctttaccct
    13381 gtcgatggac gatgtggtag tttcttcgtt cgtcaccgga ccaagttatg aaattctgcc
    13441 gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg cgctggcaac
    13501 catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg ctcgtgataa
    13561 aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct gctcgcggcg
    13621 ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac gctgtatttc
    13681 tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa agaaaccggt
    13741 attaaggcta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa gctgaaaacg
    13801 tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga taaaatgcgt
    13861 aaagaaggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag caatctcgat
    13921 ccagacatgc tcaacaagcc gtttgacccg aataacgact actccattcc gtatatctgg
    13981 ggtgcgacgg cgattggcgt taacggtgat gcggtggatc ctaaatctgt caccagttgg
    14041 gcagatctgt ggaagccaga gtacaaaggt agcctgctgc tgaccgacga tgcccgtgaa
    14101 gtgttccaga tggcgctgcg caaactgggc tactccggta acaccaccga tccgaaagag
    14161 attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc gtttaactcc
    14221 gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat ctggaacggc
    14281 tctgctttcg ttgcacgcca ggcgggtacg ccaattgacg tggtgtggcc gaaagaaggc
    14341 ggcattttct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa agaaggcgcg
    14401 ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc cgaaactatc
    14461 ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gcccggaagt ggcgaacgat
    14521 aaaacgctct acccggatgc tgaaaccatt aaaaatggtg aatggcagaa tgacgttggc
    14581 gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg ttaattcagc
    14641 aaacgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct aatgagtcag
    14701 gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccccttgta ggcctgataa
    14761 gcgtagcgca tcaggcaatg cttcctgctt tataatccct tcagcaactt ttcaacaaac
    14821 tcaggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt accgacctga
    14881 cttggttcga gattcagttc cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac
    14941 ccagccgccg gataaacatg cccggaagta ccaatggcaa tgaaaatatc agccatcgac
    15001 aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg
    15061 cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc
    15121 cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga
    15181 atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt taccagcaaa
    15241 aagcgatcgc cgagggcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga
    15301 atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga
    15361 tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca
    15421 tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc tgtcagtacg
    15481 agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga
    15541 cgcaaacgct cgcgcaggcg gcgtttattt ttacgaaaac gacttaaccg atgaccccga
    15601 cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc
    15661 attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag
    15721 agatgacgag gcagcctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca
    15781 ccaccaatga cgaccaggtc agagtcaacg atggtcagga tatttcccag acaaaccgct
    15841 aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc
    15901 gcaataattt cgggagcctg caacggttga tgataatagt gttgccagag ccacgcaaag
    15961 ccccgacctg atagataatt ttcgatacaa ccaatttgac cacagccgca gcggcgtaac
    16021 gggaaatcca gccccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg
    16081 ccggtaatgt agcttttacc ggtaatcggt ttaccgttga aaatcagccc gccgccaacg
    16141 ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc atcccaggct
    16201 tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg
    16261 ctcaggtcgg cacgcagcgg tttaccgctg gcagcgggga cattggcggc atacagcgta
    16321 ccatcttctg tttccggcat tcccggaata ccgatgccaa cagaaccttt acagccaaaa
    16381 cgttggtcag cttcggctac cagctcgcat actgcatcta aaaatgcgtc atagctgtca
    16441 cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc
    16501 gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta
    16561 ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg
    16621 ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag
    16681 ccagtgcaat tccgatggca ggaagtcaat aaaatagata tcgctggaga ggaactgatg
    16741 accgatcagc ttttcaatcc gctcaataat cggggtaagt tgcagtgaaa caactacgcc
    16801 gataatcaca ccacacaggc taccgaacag ccctgccagc aatccatacc agacaaagat
    16861 ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt
    16921 gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag
    16981 taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca tatagccgta
    17041 agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac
    17101 cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc
    17161 catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg
    17221 accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct gcatcagttt
    17281 atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct tcactttcag
    17341 cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca
    17401 ggcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt gcgggttaac
    17461 gcccttcacc tgaattgcgc gcagattggt accactttct accagtccgg tgaaattgat
    17521 atacggcgca gcggcggcaa tacccggcac tttttgcacg ttatccagtg cttcctgcca
    17581 gttagtccat ggctgattta ccgcctcaat ttcaccgtgc ggtaccacag caagaatgcg
    17641 gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac
    17701 gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc cgccgcgtcg
    17761 ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca ttactccgcc
    17821 cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg gcggctcata
    17881 cgtttcgcca gttgcaggtc gtgagtaacc accaggaagg cggtgccctg caagcgattc
    17941 aattccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggc
    18001 tcatccgcaa gtaccagacg cggattattg accagcgcac gggcaatcgc cacacgctgg
    18061 cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag ccccaccgct
    18121 tttaacatct ccagcgcacg gctgttgatt tcagcgggct ttttcttgcc aatcagcagt
    18181 ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa ctgataaata
    18241 aagcccagct tctggttgcg cagttctgct ttcgccgccg aagacagttt gctcatcggt
    18301 tgaccgttaa agatcacatc gccggaggtt ggtgtatcca gcccgcccag caggtgcagc
    18361 aaggtacttt taccggaacc agagctaccg acgatcgcca tcatttcacc ttcgccgacg
    18421 ctgaaactga cattgtgcag tacatcggtt tgcacactgc cttcctgata gcgtttgcac
    18481 aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgagtggc
    18541 ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc
    18601 aataacaatg acctgtaaag gttcgatagc gaccggcagc gccgcgccat caagcaggac
    18661 gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa gcgccgctcc
    18721 gaggatcgca ccgataatcc cggcgctggc cccttgcacc ataaagacca tcatgatttg
    18781 tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct ccattaccat
    18841 cagccctagt gaggtaataa tgttaaacgc cgcaacggcg acaatcaggc tcagcagcaa
    18901 gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac ggtcacgcca
    18961 gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa ctttcagcgg
    19021 ctcatccagc cacaaacgcc agccggtaat attgcctgcc ggataacgca tcagacgcga
    19081 ggcatcctca atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt
    19141 gccaatcaca ttgaacaggc gctggcttgg aatacgcccc atcggcgtga actggctggc
    19201 agatggtacc atcacgcgga tttgatcacc gcgattaacg cctagctgtg aggcaagttg
    19261 ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag
    19321 atacggcgta agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac
    19381 gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc
    19441 gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga
    19501 gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt
    19561 catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag
    19621 ccaggagacg aaacgaccga agcgatccgc tgcacgccca cgcatgtaac gcaggccaat
    19681 aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta
    19741 tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg
    19801 aaaagccccc gtaaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg
    19861 atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg ggtcataacc
    19921 cgcaggatca accgcatgta aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag
    19981 ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc
    20041 attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt cctgagcaaa
    20101 tgctggatct atttcaatct ttaccttgca ccctttcgta tgccggtttt tttctttata
    20161 tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa
    20221 cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta
    20281 agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc caatgccgct
    20341 tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac cagcttgtgg
    20401 tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg
    20461 ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg
    20521 tggggaatga acagtgtgat ccgcaatttg ccttattaca gccttggcgc atggtttggc
    20581 gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct
    20641 ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg
    20701 gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt cggtatgcgc
    20761 tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc
    20821 attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg
    20881 ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt catctgtact
    20941 gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct gttgttctcc
    21001 ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcgcccc agccggggcg
    21061 attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat
    21121 gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata
    21181 tcgttataca ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc
    21241 agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg tggtactcat
    21301 tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt tcaccgatca
    21361 gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt cgcctcatca
    21421 ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcgt
    21481 actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt ttctccacgg
    21541 tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac gaacccaact
    21601 ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat acgccacgcg
    21661 cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt
    21721 tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga
    21781 agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga
    21841 actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg aacatattta
    21901 ccagcaagtg agtaaaggca cattacctgc cgggatcgag tactggcagc cattgttctt
    21961 cagcgaacca ctgccgccgc tgttcagtta tttccctgcc aataccttgc tcgtgaatac
    22021 tggcgatctg gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc gttttgagaa
    22081 tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga
    22141 cgagctcttc tcagagctga aaaactggcc ccgggtgcag ctaaaaactg aacatttacc
    22201 gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc
    22261 acaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg acggtccggt
    22321 ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat
    22381 taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct
    22441 gatgattggc gctgccgaac atggttttgt cgataaggtg cgtaatctgg cgctgatctg
    22501 cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc gccgcaccat
    22561 caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca
    22621 tctggagcac ggtgtcgggc gctatgccgg aatgaccacg ctcgaagcgg gcggcattac
    22681 tggcgagtat ttgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc
    22741 actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact
    22801 tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc
    22861 ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca
    22921 cgatcgtgag cagtatcagt tgttctgcga cagcttcccg tttgaaacca cgccggatca
    22981 ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa tggatcgtct
    23041 ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc
    23101 agtagataac cacaagcagg tggcggtgtt ggtgcctacc acccttctcg cgcagcagca
    23161 ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg
    23221 tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga
    23281 tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct
    23341 gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca ttaaagcgat
    23401 gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgca cgctgaatat
    23461 ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc
    23521 agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga tcctgcgtga
    23581 aattttgcgc ggggggcagg tttattatct ctacaatgat gtggaaaaca tccagaaagc
    23641 tgccgaacgg ctggcagaac tggtgccaga agcgcggatc gccatcggtc acgggcagat
    23701 gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct
    23761 ggtttgtaca accattatcg aaaccgggat cgacatcccg acagccaaca ccattatcat
    23821 tgaacgcgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg
    23881 ttcgcatcat caggcatatg catggttgct gacaccgcat ccaaaagcga tgactaccga
    23941 tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag gttttgcgct
    24001 ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag aacaaagcgg
    24061 ctcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc
    24121 actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga
    24181 gctgcggatg ccgtcgctat tgccagatga tttcattcct gacgtgaata cgcgtttgtc
    24241 gttctataaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga
    24301 gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg atattgcccg
    24361 tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg
    24421 ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca
    24481 aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta ttcaggattt
    24541 gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa
    24601 cgcgatcgct taatggtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa
    24661 ttctttgcac ttccctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt
    24721 ttattacaat aaccttatat ttattattga tttttggtga tgatcaaaac gcgtttttct
    24781 cgctggctaa cgttttttac gttcgccgct gccgtggcgc taccggcaaa agccaacacc
    24841 tagccgctgc cgccagcgag cagtcgtctg gttggcgaaa acaaatttca tgtggtggaa
    24901 aatgacggtg gttctctgga agccatcgcc aaaaaatata acgtcggctt tctcgctctg
    24961 ttacaggcta accccggcgt tgatccttac gtaccgcgcg cgggcagcgt gttaacgatc
    25021 ccgttgcaaa ccctacttcc agacgcgccg cgcgaaggca ttgtgatcaa cattgcggag
    25081 ctgcgtctct attactaccc gccgggtaaa aattcggtaa ccgtgtatcc aataggtatt
    25141 ggtcagttag gtggtgacac gctgacaccg acaatggtga ccaccgtttc agacaaacgt
    25201 gcaaacccaa cctggacgcc aacggcaaac atccgcgccc gttataaagc acagggaatt
    25261 gagttgccta cggtagtgcc ggctggaccg gataacccaa tgggccatca tgcgattcgt
    25321 ctggcggcct atggcggcgt ttatttgctt cacggtacga acgccgattt cggcattggc
    25381 atgcgggtaa gttctggctg tattcgtctg cgggatgacg atatcaaaac actctttagc
    25441 caggtcaccc caggcaccaa agtgaatatc atcaacactc cgataaaagt ctctgccgaa
    25501 ccaaacggtg cgcgtctggt tgaagtacat cagccgctgt cagagaagat tgatgacgat
    25561 ccgcagctgc tgccaattac gctgaatagc gcaatgcaat catttaaaga tgcagcacaa
    25621 actgacgctg aagtgatgca acatgtgatg gatgtccgtt ccgggatgcc ggtggatgtc
    25681 cgccgtcatc aagtgagccc acaaactctg taaatcctgg ataaaaaaaa tccccgcgac
    25741 acggcatgtc gcggggaaaa aatcagcagt agcaggcatt aatgagggtt aatgcttatt
    25801 tataaattac tgctgttcca tggagggtat tcggaccggt tacagaagta atacggaaag
    25861 attttgcgcc catctcatcc gctttttgcg ccagctgctc ttccagcgat cccagatttg
    25921 tccccgcgtt agcactgata gtaccgactt tttgttggcc ttctggcgtt gactgaactt
    25981 cgacagccgc aaagttggca aatgacatgg agcttaaaat cgccgcagcg atgagggttt
    26041 ttacgttttt cataatagtg gccttatgca gatgaatgac gtcgcaagaa attacttaac
    26101 gatcgataac taaatgatag atgtgatctg gatcacatac aagatatttt ttataacaat
    26161 cattaattaa tttaaaaatc tttaaatttc agttaaatag aaagcaatta tttttatggg
    26221 atctggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
    26281 gttcaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc
    26341 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg
    26401 ttatgaagcg acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct
    26461 atacgcggaa tttaccaaca aagaggggtt atttcgtgcc gttctcgacc gctatatcga
    26521 tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
    26581 gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg
    26641 ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac
    26701 gttgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
    26761 agcgcgcggg gaaatcccga cccactgtga cgtgacacat ctggcagaat tccttaattg
    26821 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactgatgca
    26881 gattgccgga acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaagc
    26941 ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta
    27001 ttcaaaatca gttgcccgtt actatctagc gggatctgcg tacccggatc gcggtccatg
    27061 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
    27121 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
    27181 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg
    27241 acatctttac cacgaccacc accaaactga tgccctatca cgccgccagc aacagcgccg
    27301 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg
    27361 ttgcgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
    27421 tgagcgtatt gcgggccccg ttcaaacacg ttcagactgg ccactgccgc tacgcccagc
    27481 gcagctgcga caccaatccc gatacccgcc aacattgatt tattcacggg aacacctcct
    27541 tctttgcagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
    27601 aaaagcgcgg gaatggctgg aaatacttaa ggattcggag aagtctgaag gaatgcctga
    27661 tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggatga
    27721 ggcgtttatg ccacatccgc cagtgtacgt cgattaatgc aacttcaaac gcggacggat
    27781 aacgcggtta atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc
    27841 aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat
    27901 cattgagccg cgcgtcaggt tacccatcag gctaccgaca gtggagaagt tcgacagcga
    27961 taccagcgaa ccatgatctt tatactgata atttttcagc ggtttaccgt tcatctgcgc
    28021 cagaatattg ttcattgcgc aggtcgccat ctggtgtgca gcctgagcac gcggcggaac
    28081 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc
    28141 gcgggtggtt tgcagcgtcg gttccaccac cagctggttg atacggttgg tttcaagacc
    28201 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc
    28261 ctcaatatat tcaccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
    28321 ttgcgtcagc acgcgaacgc caagtttcgt tagctcgttg tgggccgcag cagagatacg
    28381 tggcggtaat gcaggcaaaa tacgttctcc cgcttctacc agcgttacgt tcagggcttc
    28441 gttggtcagg cctttgtaac cgtagctgtg cagttgcttg accgcattgt gcaattcagc
    28501 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgttcactt taccattcgc
    28561 gcccaggttg gcggagtatt tcaggaacag attcagcatc tcctggtgga agcgacgcgc
    28621 ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt
    28681 agaggtgcta cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag
    28741 cagttcacct ttctcatcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat
    28801 atcaatgacg gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct
    28861 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca
    28921 cagatggctg tggttacgat cgaccagcgt aattttggct tttttcttgc gtcccagctt
    28981 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatttt
    29041 tttcaatggc gtagtcaacg tgaccccctt aaatttatta accaattgtt aattaatggt
    29101 tataaacata gctcttaatt aacaacaggt tacagcaatg aaaatgttct tcgctattga
    29161 gaataacatg aatggtgcat tggtcatacc aaaattgatg ttaatcaagt tttgttgctg
    29221 aaaagatagg cgactttacc gggtgagatc aaaaaacaga gtttattgtt tattatttga
    29281 gcaagtccgg aagggtgata tagggtgacg cgtatatcga gggcgtttgg tcgtcctgat
    29341 aagatgcgca gcatcgcatc aggcattgat gctcgactgt cggatgcggc gtaaacgcct
    29401 tatccgactt acagaattcg gagcgcatca ggtaccggca aggttacctg atgcgacgag
    29461 ggcatttacc cgagggtttt gaacgcctta atgcgctgca aatgcgggga gatattcttg
    29521 aatttgtgcg tctgctcttc gtcccagaca atctcgtaat aatgatgcaa ctcttcagat
    29581 gtccgctggc tgttaagcgc ttcatcatta cgcgacaaaa tcaccaggca acgatcgcga
    29641 ttcttctcgc ggaagttggt cacacactta gtggcaatat cggcatactc ttccgggcga
    29701 tcaatcttgc cttccatgtt ctcataaggg aacaaattag ggttgaagat cacctggcgg
    29761 atatcgcaaa gaaaaccaat ccgttccgcc cagtatccgc ccagaccaac gccgcaaatc
    29821 agcggacgct cgtcgacgtt cagttgcaac attttgtcca cttctttgag cagatgctgc
    29881 atatcatgtt tcggatgccg cgtactgtag cttatcaagc gtacatccgg gtcaataaac
    29941 tgcaattgta agactttctc gtggttaccc ggactgttag agtcaaaacc gtgtaaatag
    30001 ataatcatcg cttcctcaca taagccaggg ttagtgacct gctttctctt cctgccagcg
    30061 ttcatgtaac tgattcagac gggtgctgat cgccttccag cgagccgagt ccatcagttc
    30121 ctgtcgcgaa aatgaacctt tatgatacaa acgtgtaaca cgttctgcct tgatcggtga
    30181 cagattatct aacacgctga cggccccttt acgattattg cagaccagga tcatatcgca
    30241 acctgcgtcc agtgatgcct gcccgcgttc ggcataactg cccataatcg cggcaccttc
    30301 catcgataaa tcgtcagaga aaattacacc gtcaaaacac agttcctgac gcaaaacggt
    30361 tttcagccag taggaagaac cgctcgccgg acgcggatca acatcactgt agatcacatg
    30421 cgcaggcata atggcgtcga gtttattttc gcggattaag gaactgaaga ccgacatatc
    30481 tttagcgcga atctccgctt gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc
    30541 cgttactgca ccgtgtcctg ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat
    30601 accatcaata aaccggctgg caattgccag ggctttttgt ggatcggcat gataagaacg
    30661 ctcgccaatc gccgcgctga tatgcccgac atccagcact ggcgcaaagc tgatatcaat
    30721 atccatagcg atcatttcgc tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc
    30781 ctcttccatt cccaacagcg cagcaaagga ttgtgctgcc ggtaagcggg taaaaccttc
    30841 gcgaaaacgc tgcacgcgtc caccttcctg atctaccgcc accaccagat gattgcgcga
    30901 tgctgcgcgg atctggcgca ccagttcacg taactgggca ggatcatgat agttacgcgt
    30961 aaagagaatc agccctccca ccagcggatg cgccagtatt tcacgctctt ccgcgtccag
    31021 ttcgtaacct tcgacatcca acattactgg acccacactg ctctccttat tgttttatta
    31081 atagctgccg ccaggtgtca tcggccagcc tgataaattg ttgatcgccg atttgtcgcc
    31141 agcggtactc aaaccaccct gctttgagca tcagcagcca gggaaaccat cgcctgacct
    31201 gacgccataa ttgcgccgga taaatcttcg cgcgagtggc atagtcattg accaattgcc
    31261 ggtgctgttc agtattttcc acccacaccg ccgccagttc cagcgcgata tcaccatctc
    31321 cggcatactc ccagtcgatg agttttaacc ctgacgcgct atgcactaaa tttccggcgt
    31381 ggacatccat atgcaatgga cttaagcgta aaggccgtgg ttcccgcgct ttgcgcagac
    31441 gttttaacat tcgcagccaa cccacagtcc gccgcgccgg atcgctttgc tgccagtaca
    31501 gttccagtaa gggcaacagc gttattcgcc agccaaaacg tggttgttga tgaagataat
    31561 acagcaagcc tgccagttcg ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat
    31621 agtcgactac catccagtca cggagatata aatgcggctt cggtgcaatg cttgcgggta
    31681 gttgtgataa agcccgatac tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg
    31741 gctgacgcac aacaaaacgc tggccctgat gttcaatgag aaaactaccg ccactaagcc
    31801 cactattaaa cgtcgtgacg ggatgaaact gcgggaaaaa gcgcgacagc aattcgtcgc
    31861 gcgtgatggg attattgctg cgaaacggca cctttacctg accagataat ttcgcccgtc
    31921 tgcaccagca tcagctgcat ttgtagggtc ggagcgttaa cgttgccaga ggcgctggag
    31981 tacagcacgt aatgagcgcc gacattgcgg gcaatgccta tggctttact acgggtacct
    32041 aaactgtcct gcggcgacaa acctaactgt tgcttagcca tcgacagctg ctgggcggaa
    32101 accagggtaa atttcccgtt attagccagc gcatttcgca gcgtttcggt cgcttctgcg
    32161 gcattcagcg aaccgttagt acggttgtta acgctatcaa ccagcaggac gctacccgca
    32221 gtcaccccgt cagccccaag catcttactg accatcggct gcattgcgcc attccagtca
    32281 taatggcgaa tatgcggcgc aggcggtgcc gtttgatctt cgtgctcaat tgggcctggc
    32341 tgctgcggga tcgtcggcac cgagggcact gtggggacag gctgttgtgg ctcggctggt
    32401 tgttccggcg ctggtttcac ttcttctacc ggtgcaggtt cacgttgccc cacacacccg
    32461 gcgagaaaca tcgccagcgc ggtaatcaag gcgtagcgac tcatttttgt catcaagatt
    32521 caccccttac aaatatagat aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc
    32581 gtacagcgtt accgccgaat gtgcgggaat gctaacgctg cgtggccttt ccagaggatg
    32641 catctccagc cctctggcgt cataccagta aaaacgataa tgaacggtaa cgggttcttg
    32701 cctttcgtta taaagcgttg aggatgctga aggttgaata tcagacgtcg aaaggacggg
    32761 cttttctgca ctgatgcccg cagccaataa agatgactcc atcaccagcg attgctcatc
    32821 attcaccgga atttccggat gtgaacgaca gcccaccagc agtaacaacg ccagagacac
    32881 cagcccaaag catccttttc tcatcgttac agacctttat gcgccagcat tggtcccagc
    32941 ggacggccac ccaacaagtg catatggatg tggtaaacct cttgtccgcc atggcggttg
    33001 gtattcatga tcagacgata gccatcttcg gcaatacctt cttgctcagc aatttttgcc
    33061 gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag ctgacacgtc gttcacagtc
    33121 ggaatgagga tattcggaat gatcagaata tgcgttggcg cttgcggcga aatatcgcga
    33181 aacgccgtta ccagatcatc ctggtagacg atatcggagg ggatctcacg acgaataatt
    33241 ttgctgaata tagtttcttc tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg
    33301 ccatgctacg ccgcgccaca ctgcgagtat agagtatggt cgagttaaac ctgctctttc
    33361 aaccttaact cacgatttct taagcaaaaa atcgttttgc tgccgtatat atcgccatta
    33421 ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc tgtttggttt acacgacaaa
    33481 tatgaatgcg catatttctc atttgcattt acaaacaaaa ttattcgcac actaaatata
    33541 accgctcatc accctcctcc ggcatgagcc tgacgacaac ataaaccaag agatttcaaa
    33601 tgctttcaac acaatttaac agggataatc aatatcaagc catcaccaaa ccgtcactac
    33661 ttgccggttg catagcactg gcactattac cttctgccgc ttttgctgca ccagccactg
    33721 aagaaacggt gattgttgag ggttcagcca cagctccaga tgatggcgaa aatgattaca
    33781 gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac tcaacttgat attcctcagt
    33841 cggtcactat tgttagccag cagcggatgg aagatcagca gttacaaacg ctgggcgaag
    33901 tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt
    33961 attcccgtgg attccagatc gataactata tggttgatgg tatccccacc tattttgaat
    34021 cgcgctggaa tctgggcgac gcactttctg atatggcact ctttgaacgc gtagaagtag
    34081 tgcgtggcgc gacaggactc atgaccggga cgggtaatcc atctgcggca attaatatgg
    34141 ttcgaaaaca cgcgaccagt cgtgaattta aaggcgatgt ctcggcggaa tacggtagct
    34201 ggaacaaaga acggtatgtg gcggatttac aaagcccact caccgaagac ggtaaaatcc
    34261 gcgcgcgaat tgtcggcggc taccagaata acgactcatg gctggaccgc tacaacagtg
    34321 aaaagacctt cttctcgggc attgtcgatg ctgatttagg cgatcttacg acgctttcag
    34381 ccggttacga atatcagcgc attgatgtta atagcccaac ctggggcggt ttaccgcgct
    34441 ggaatactga tggcagcagc aacagttacg atcgtgcacg cagtaccgca cctgactggg
    34501 cgtacaacga taaagagatc aacaaggtct ttatgaccct gaagcagcgg tttgctgata
    34561 cctggcaagc gacactgaat gccacccact ctgaagtcga atttgacagc aaaatgatgt
    34621 atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt tgggccatac agtaattatg
    34681 gacctggctt tgattatgtc ggcggcaccg gttggaacag tggcaaacgt aaagttgatg
    34741 cgctggattt gttcgctgac ggtagttatg aattgtttgg tcgtcagcac aatctagtgt
    34801 ttggtggcag ttacagcaaa caaaacaatc gttacttcag ttcatgggcc aacatcttcc
    34861 cggatgaaat tggcagtttc tacaacttta atggcaattt cccacaaacc gactggtcac
    34921 cacagagcct ggcacaggac gataccacac atatgaaatc gttatatgct gctactcgag
    34981 tcacccttgc cgatccgctg catctgatcc tcggcgcacg ttataccaac tggcgggttg
    35041 atacgctgac ttacagcatg gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg
    35101 acatcaatga caactggtcg acctacgcca gctatacctc tattttccag ccgcaaaatg
    35161 atcgtgacag ttcaggcaaa tatctggctc caatcaccgg taacaactac gagctgggtc
    35221 tgaaatcgga ctggatgaat agccgtctga ccaccacgtt agccatcttc cgtattgagc
    35281 aggataatgt cgctcagtcc accggtacac ctatccccgg cagcaacggc gaaaccgcct
    35341 ataaagcggt ggatgggaca gtcagtaaag gggtggaatt tgaactcaac ggcgcaatta
    35401 ccgacaactg gcagctgaca tttggcgcaa cgcgctatat tgcagaggat aacgaaggaa
    35461 acgccgttaa tcctaatctg ccacgcacca cggttaaaat gttcaccagc tatcggttgc
    35521 ctgtcatgcc agagttgaca gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg
    35581 acaccgtgac accgtatggc accttccgcg ccgagcaagg tagctacgcg ctggtggatc
    35641 tcttcacccg ctaccaggtg acgaaaaact tctcgttaca ggggaacgtc aataacctgt
    35701 tcgacaaaac ctacgatacc aacatggaag gttctatcgt ctacggcgca ccgcgtaatt
    35761 tcagcattac cggcacgtat caattctgaa taacacctgt aaaaaaggca gccatctggc
    35821 ggccttagtc tccccaacgt cttacggatt agtggttacg gatgtactca tccatctcgg
    35881 ttttcaggtt atcggattta gtaccgaaaa tcgcctgaac accagaacca gcaaccacta
    35941 cgcccgctgc gcccagtttc ttcaggccag cctgatccac tttagacaca tcagcaacgc
    36001 tgacgcgcag acgggtaata catgcgtcga ggttagtaat gttttcttta ccaccaaatg
    36061 cagcaaccag agccggtgcc atttcgctgg tacctgttgc ttttgcatct tcagtcgcgt
    36121 cttcacgacc cggcgttttc agatccagtg ctttaatcag cacgcggaag atggtgtagt
    36181 aaacaatcgc ataaccgata ccgacgatcg ggaacagcca cagtttgctg ctgttaccag
    36241 acagaacgat aaagtcgatc agaccgtgcg agaacgacgt accgtcacgc atccccagaa
    36301 gaatacagat tgggaatgcc aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg
    36361 caacgaacat gaaggagaac tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg
    36421 ccgcggagat cataataccg cccactttcg cgcggttttc tggtttagca gagtgccaga
    36481 tagcaattgc ggcagctggc agaccgtaca ttttgaacag gaagccacca gacagtttac
    36541 ccgcagtcgg gtcacccgcc atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg
    36601 cgttggtgta ttcaccaatc tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa
    36661 acggcaccag gcaacgttcg atgaaaccgt aaatgccaaa cgcaactacc gggttctggt
    36721 aagcagccca ctgagagaag gtctggattg cagaaccaat cggcggccaa atgaaggaca
    36781 gcacaacgcc agtaaagatg gcagccaggc cagaaatgat cggcacaaag cgtttaccgg
    36841 caaagaagcc aagatactca ggcagcttaa tacggtagaa acggttaaac atgtacgctg
    36901 cgatcgcacc ggagataatc cctccgagta cgccagtatc cgccaggtgt ttagaggcga
    36961 tttcttcagc aggtaaatgc agtaccagtg gcgcaaccac ggccatggtt ttaaccatga
    37021 tgccataggc aacaactgcg gccagcgcgg atacgccatc gttattggta aagccgaggg
    37081 cgacaccgat cgcaaaaatc agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca
    37141 taacatgcga tacaacggcg ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa
    37201 tacctgcgat aggcagtacg gataccggca gcatcagcga tttaccgacc ttttgcaggt
    37261 tagcaaatgc attcttaaac ataattgaga gtgctcctga gtatgggtgc tttttttacg
    37321 ttctcacgcg tggcaagggg ggagagcctc gccgtgtaca gggcatctaa gcgcccttta
    37381 tttattacac agagtaaaat aattcagtgc cgatttgttt gacggctatc acgtttcaac
    37441 ttcagatcat tggagaagtt gcacattttg acaaaacata tgtcacaata tttcaaacgt
    37501 ggttattcac cgcccaattt tcggcggtga actttactcg ttttagccat taattacgag
    37561 ctttaaaaaa actcattcaa cggatggatt gaaggcggga agcgtcgatg tgaaacagac
    37621 gggcgaagtt atcggtggtt acctgcgcca gttcttcaac ggcaacacct ttcaacacag
    37681 ccatgtattc tgcaacgtca cgaaccatcg caggttgatt ctcttttcct cgatgcggta
    37741 ccggcgcaag gtaaggtgag tcagtttcca ccagtaaccg atccaggggg acataacgcg
    37801 cagcatctcg aagttgctcc gcattacgga aggtcacaat gccggaaaag gagatgtaaa
    37861 atccgagatc cagtaattta cccgccgttt ctctgtcctc tgtaaaacag tgtagtacgc
    37921 caccgcaatc cgtcactttt tcttcgcgca gaattgccag cgtatcggcg cgggcgtcac
    37981 gggtatggac gataaccggc ttgttcagtt cacgaccaat ctggatatga tggatgaagg
    38041 actcttgctg acgaacttta gtttccggtg tgtaataata atccagcccg gtttcaccca
    38101 gcgctacaac accctcttct gccgccagac ggcgtaaatc ttctacatcg tagggatcat
    38161 tctggttgag tggatgcacg ccacaagaaa atacgacgtt gtcacgttcg cctaccagat
    38221 cccgcatatg taaataaccc ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc
    38281 gtgcggcggc tttcgccaga acgtcatcca cgtccttatg caaagattca taatccagac
    38341 catcgagatg gcagtgtgag tcgactaaaa acatgatgtc tctcttaaag atgaggaacc
    38401 ggtagcacaa cgcccggttg caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga
    38461 agctcgcggt tgatgcctgt gacagacatt aactgttcac gaatgtggca aacatccccc
    38521 agtatagcct gcaggcgcga gggagaaaga tggtttgcca gttcgacgac aaggcccggc
    38581 acatcaacat tggtcacctg cgcagcacca tgatggcgtt ttagcgcatc catcagcaac
    38641 gttgccagcc agtgtaaacg cgccggagct tgttcatgat taagggccgc cagcagcgaa
    38701 taccagtcgc ccgatggcac gctatatgcc aatgcctgac acaatgtttc acgagcctgc
    38761 cagttatctc cctgaaacaa cgccagtgcc gcgccaggcg aaccggcgct taagcgcaat
    38821 gcggcaagta atgcatcctg tgacattgtc acttcgcgtg aaagccaggt cacggcgtac
    38881 tgttccggcg gcggcgcaag gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa
    38941 cgttcaggct cgcgggtagc caggaaaaac caggtttctg ctggtggctc ttcaagcgtt
    39001 ttcagcaatg cgttagccgc ggcgtcggtc agtaaggcag catcggttac ccagacaact
    39061 ttcgcaccac ctaggcgtgc gtgctcattc agcttttcgg tgacctcacg tactgcatca
    39121 atgcccagcg tatttttccc tttttcggga gccagggtgt agtaatcggg atgcgtgcca
    39181 gcctgcatca actgacatcc acgacagtga ccgcaacttt tgtggccctg cggttgttgg
    39241 cagagtaaat aacggctcag ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc
    39301 tgaatgagta gcgcatggtg acctcttccg gcctgatagc tggctaccag tttttcgaaa
    39361 tcaggtcgta accatggata ccatctcatg cgtccaactc cttcacccag tgggtcacgg
    39421 tagtgcggat tgcatccatc acggcctcca gcggctgggt ggcatcaatg gtatgaatac
    39481 ttttatcttg tgcagccagt tccagatagc gggcgcgggt gcgattaaag aaatcgaaag
    39541 actcttgctc aatacgatcc agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg
    39601 gggtaacatc gagatagagc gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca
    39661 gtgttgccag catatgttgg tcaataccac gtcctccgcc ctgatacgcc tgagtggaga
    39721 gatcgtggcg atcgccaatc acccaggtgc cgttagccag agctggtttg atgaccgttt
    39781 ctaccagttg aacgcgcgcg gcataaaaca tcagaacttc ggctttatcg gtaatgactt
    39841 catcgcctac cgatttgata tccagcacca ggcttcttaa cttttcggca agttgcgtac
    39901 cgccaggttc ccgagtgaaa accatgtcgc ggatacccag ttgctcaagc gtctcaacca
    39961 ccacattacg cgcggtagtt ttgcctgcgc cttccagccc ctcaatgacg atatacttac
    40021 tgcgcatttt tttccttaag cactttcaga taatcctgca cagacttgtt atggctggca
    40081 agattggtat taaacgtgtg accaccttta ccatcggcca caaaatagag atacggcgtt
    40141 tttgccggat gcgcagcagc cttcagcgaa tccgcccccg gcgtagctat cgcacccggc
    40201 ggcaaaccgg taatggtata ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa
    40261 agtttgccat tataacgctc tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc
    40321 ataccaatgc gtaaacggtt gataaatacc gaggcaacct gatcgcgttc actggcaacg
    40381 gcggtttctt tttcgataat tgatgccatc gtcaccagct gatttttatc tttataaggc
    40441 agaccgtccg cacgcccttc ccaggcgcta tcgaccgctt tcaccatttt cttgtgcgct
    40501 cgcttgagta acgcgacatc ggtggtattg gcggtataca tccaggtgtc tggccagaac
    40561 caaccttcaa tccactccgg gttttccagt tcaagtgcct gcgctacggt ggcgtactta
    40621 tcgtcactca gcgtgtgctt gatatacgga gcctcacgca attgcttgag gtagtcgctc
    40681 agacgcatcc cttctaccag tcgcagcggg aactgtgctt ctttaccgct ttccagcaat
    40741 ttcagcatct cgcgcacggt catctgcggt gtaaagcggt aagtcccggc tttaaagtga
    40801 gaaagatccg gttcgatacg cagcaaccat tgaaaaaccc gtgggcgatt gatgatctta
    40861 tcggcataaa gctgttcacc aagcgccaga cgtccggtcc ctggcttcag ggtaaatatc
    40921 gtctcttctt tgataagcaa tttgctgtcg gcaagatggc gaaccttcca gacgcccaca
    40981 ccagcggcga tacccagtac caccagcaat aacaagataa tcaataacac ttttttcatg
    41041 actaattcgg gcgctcacaa agtggggcta aatattcata taacgttgct gacgaaaagg
    41101 agacatcgcc acaggcacgt acgggcatca ctggcattaa cgcattacaa ataaccatct
    41161 catctgcctg caacgcctct tccagagagg cttgcacttc gacaagctga taagaggatt
    41221 gtgccagcaa acggatacag aattgtcgca taatgccgtt aacacctgcc tgatccagac
    41281 gcggcgtata aactacattg cccttccgcc agaacaaatt agccgcacag cattccgtaa
    41341 cccacccttc gctgtcaagg accagcgcct catcagcgtt tgtctgctca agatgagagc
    41401 ggatcaatac ttgctcaagc cgattaagat gtttaatacc tgcaagatga ggattgcgcc
    41461 ccagccgcac cgggcttagc gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg
    41521 caggataaac cgtaacggag agaatccgcg ttgccggtcc actgttcaat gtgctgtacc
    41581 ctcgcccgcc actaccgcga ctgatcacga ctttcagcac accattttgc tgttctgctg
    41641 ccagcgtttt catctcttgt tcaagctgag gccagaagtc acagaaaatc atcaaccgct
    41701 gacaagcatc ctgtagtcgc tggatatgcg ccgataacaa actgacttta ccgtcgataa
    41761 ctctggcggt ggtaaaacaa ccatcaccaa actgcgttgc ccgatcgctt actgccagcg
    41821 attcctgctt atgaccgtta attaagaaca taccggctcc ttatcagaga attgttagtg
    41881 tggcagcatg ttcactacgg aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc
    41941 ggacctttta tatgggtggg aaatgacaac ttagatcttt ttaaagatca aagaaccatt
    42001 agtgccaccg aagccgaagg agttacacag cgtgtattcc attccgctga cctgacgtgc
    42061 ttcatgcggt acgaaatcca gatcgcaacc ttcatccggg ttatccaggt tgatggttgg
    42121 cggaacagcc tgatcgcgca gcgccaggat ggagtagata gattctactg cacccgccgc
    42181 acctaacagg tgaccggtca tagatttcgt ggagcttacc aacacacggc ttgcagcttc
    42241 accgaagatg gttttcaccg cctgcgcttc agctttatcg ccagccggcg tagaagtacc
    42301 gtgcgcgtta acgtagccaa tctgactcgc ttcaatgcct gcatcacgca gagcatttgc
    42361 catcgccaga gctgcgcctg cgccattttc tggcggtgac gtcatatgat aagcatcgct
    42421 gctcatacca aagccgacga gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc
    42481 gtactcttca agtaccagca taccggcacc atcgcccagt acgaagccat cacgctcttt
    42541 atcccacggg cggctcgccg cttgcgggtt atcattgcgg gtagataatg cacgtgccgc
    42601 gccaaaacca ccaacaccca gcggcgtact ggctttctct gcgccacctg caaccatcac
    42661 gtcagcatcg ccatacgcga taatacgcgc agcatggcca atgttgtgca cgccggaagt
    42721 acaggcggtc gcgatagaga tgcttgggcc acgcaggcca tacatgatag tcagatgacc
    42781 tgccaccatg ttcacaatcg ttgacggaac gaagaatggg ctgatcttac gtggaccacc
    42841 gttcatcaga gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc cggagccaat
    42901 tgcggcacca atgcgggttg cgttctcttc cgttatatca aggccagaat cctgcatggc
    42961 ctgaacgcca gcgacaattc catattgaat gaaggcatcc atcttgcgct gttctttgcg
    43021 cgagataatg tcctcacagt taaaatcctt tactaagcca gcaaatttcg ttgcataggc
    43081 gctagtatcg aaatggtcga ttaggctgat gccactctga ccggcaagca gagctttcca
    43141 ggtagactct acggtattgc cgacaggaga caacatgccc agtccggtca caactacacg
    43201 acgcttagac acgtttgtcc tccagggagg gaaaaaatga ttctagtggg acaaaaagat
    43261 aaaactcagg cggtcgaacg accgcctgga gatgttcact tacgcctggt ggccgttgat
    43321 gtaatcaatg gcagcctgaa cggtggtgat tttctcagct tcttcgtccg gaatctcagt
    43381 atcaaactct tcttccagag ccattaccag ctcaacggtg tcaagagaat ccgcgcccag
    43441 gtcttcaacg aaagaagcat tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc
    43501 gataattttc ttaacgcgtt cttcgatagt gctcatactc ttaaatttcc tatcaaaact
    43561 cgctttcgcg atggttttcg tagtgtataa aatgttgaaa aatttgcaac taaatcccgg
    43621 caggtcttac cacgatttta cgttattttg aggccttacc ccccaataac gcaaatcatt
    43681 ttgcactaat tattactcat aaccacgcag actgcgcgca acatgagcat tttgtgcaaa
    43741 tcgcggtcag accatgtaca tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc
    43801 tgcttcgtcg gatgccagga atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg
    43861 acccgcagga acctgcgcca ggatacccgc acgctggtca tcgctcagcg cacgtgtcat
    43921 gtccgtttca ataaagcccg gagcaacaac gtttacagta ataccgcgtg acgcaacttc
    43981 gcgcgccagt gatttactga agccgatcaa gcccgctttc gccgcagcgt agttggcctg
    44041 accgccattt cccatggtac caaccacaga accgatagtg ataatacgac catgacgctt
    44101 tttcatcata gcgcgcatta ccgcttttga cagacggaaa acagatgaaa ggttggtttc
    44161 gataatatcg ttccactctt catctttcat tcgcattaac aggttatcac gagtgatacc
    44221 ggcattattg accaggatat ccacttcacc aaattctgcg cgaatttttt ccagaacaga
    44281 ttcgatagat gccgggtcgg tcacattcaa catcagacct ttgccgttgg cacctaaata
    44341 atcgctgatc gcctgagcgc cattttcact ggtcgcagtg ccaataactt tcgcgccacg
    44401 ggctgcgagc gtttcagcaa ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc
    44461 gatttttcct tcaaaattca tgattttcct cttttaaagc tcaagcgccg ctgccatcgc
    44521 tgaaggttcg ttcagcgccg aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt
    44581 aagcaccttg cccgggccga cttcatagag atgttctacg ccttgcgctg ccatgtactc
    44641 aacagacttc gtccactgaa ccgggttata caactgacgt accagtgcgt cacggatggc
    44701 atcaccattg gtttcgcatt tcacatcaac gttattcaca acagaaactg ttggtgcgtt
    44761 aaaggtgatt ttcgctaatt ctactgccag tttgtcggct gctggtttca tcagcgcaca
    44821 gtgagacggt acgctcactg gtaacggcag agcacgtttt gcgcccgcag ctttacaggc
    44881 agcgccagca cgctcaaccg cttctttatg accggcaata accacctgtc ccggagagtt
    44941 aaagtttacc ggagaaacga cctgacctgc tgcagcttct tcacacgctt tcgcaataga
    45001 cgcatcatcc agaccgatga ttgccgccat agcgcccgta ccttccggta cggcttcttg
    45061 catgagcttg ccgcgcatct caaccagacg caccgcatca gcgaaatcaa tcacaccagc
    45121 gcaaaccagc gcggaatatt cccccaggct gtgaccggcc atcattgccg gtgctttacc
    45181 gccctgctgc tgccatacgc ggtacagcgc aacagaggca gtcaacagcg ctggctgggt
    45241 ttgccaggtt ttattcagtt cttcagctgg cccctgctgg gtcagcgccc acaggtcgta
    45301 gcccagcgcc gcagaagctt cagcaaacgt ttcttcgaca attggatagc tcgccgccat
    45361 atcagccagc attccaacgg tttgagaacc ctgtccaggg aacacaaatg caaattgcgt
    45421 catgttttaa tccttatcct agaaacgaac cagcgcggag ccccaggtga atccaccgcc
    45481 aaaggcttca agcagaacca actgccccgg cttaatgcgc ccgtcgcgta cagcttcatc
    45541 cagcgcgcac gggacagagg ccgcagaggt attaccgtgg cgatccagcg tcaccacgac
    45601 attgtccata gacataccga gttttttcgc cgttgcactg ataatacgca ggttagcctg
    45661 atgcggaacc agccagtcca gttgagaacg gtcaagatta tttgccgcca gcgtctcatc
    45721 aacgatgtgc gccagttccg ttaccgcaac cttgaagact tcgttgcccg ccatcgtcag
    45781 atgaattgaa ttctctggat tcacacggtc agcattaggc agcgtcagca actcaccata
    45841 gctaccgtcg gcatgcagat gggtggagat gattcccggc tcttcagagg cagccagcac
    45901 cgcagcgccc gcgccatcgc caaaaataat aatagtccca cgatcggttg gatcgcaggt
    45961 gcgcgccagt acatcggaac cgacgaccag agcatacttc accgccccag atttcacgta
    46021 ttgatcggct acgcttaatg cataggtgaa acctgcgcag gctgctgcaa cgtcaaatgc
    46081 cgggcaacct ttaatgccca gcatgctttg aatctgacaa gctgcgctcg ggaaagcgtg
    46141 cgtagcagaa gtcgttgcca caacgatcag gccaatctgg tctttctcaa tgcccgccat
    46201 ctcaattgcg cgtgtcgccg cttcaaagcc catggttgaa acggtttcgt ttggcgcggc
    46261 aatgtggcgt tcgcggatac cggtacgagt gacaatccac tcgtcagagg tgtccaccat
    46321 tttttccaaa tcggcgtttg tccgcacttg ttcgggcaga tagctgccag taccaataat
    46381 cttcgtatac atgtacgctc agtcactttt cggttatata ccgtcacttg caaactgcga
    46441 gttcgctggc agcgtcctgc taccgcagag ttccgctttt gccaccgtcc agcagctcaa
    46501 aaccagctgg gtatacagat tccaggcgag cggcaattcg ctgaggaact tgtcgctgca
    46561 ccgcctgcac tgcctgttca atcgcgaccg caaaagctcg ctgattggct gcaccatgac
    46621 tttttatcac cgtgccgcgc aatcctaaca gacaggcgcc gttatactgg tcggggttga
    46681 ggtgactgaa tcgcctcgtc aggctctttt gtagccaacg ctttaataac agtagccacc
    46741 acgaccgttt tttcccttca ccctgagatt tcagcagaga aaggaacatc ctgacaacac
    46801 cttccatcgt ctttaatgtg acatttcctg taaagccgtc acaaaccagc acatctgtct
    46861 tgccagttaa caactcattg gcttcaagat agccgatata attgatagaa gggattgttt
    46921 taagcaccgc tgaggcatcc cgaatactgt cgagaccctt tacttcttct tcaccaatat
    46981 tgagcaacgc cacgcgagga ttgggaattt ccaccacctc ttcagccaga actgagccca
    47041 taatggcaaa ttgcaccaac attgtactgt cacaatcgac gttggcccct aagtcaagga
    47101 ccaccgtttt gcccttttgc tgatgtggta ataccgtcac cagcgccgga cgctcaatcc
    47161 cctccagggg cttgagtaat aattttgcca gccccatcag tgccccggta ttaccggcac
    47221 tgacacaggc ttgcgctcga ccttctttca ccagctccag ggccacgcgc attgaactcc
    47281 cacgactggc gcggatagct tgcgaaggcc gggcatcact ggcgataact gactgcgcag
    47341 gaataatctg cagacgcgaa cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg
    47401 cgtcgggatt gccgactaaa agaagagtga gttgcgaatt agaattcagt gcctgcaatg
    47461 ctgcaggcac tgtcacggaa gggccaaaat cccctcccat gacatctaac gccagggtta
    47521 gacgtgtcaa ggtatcgtcg ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc
    47581 ctcactaagc ttcatcacgc agacgcgtga ttacttagcg atgaccttgc ggccgcggta
    47641 gtaaccgtcg gcagtgatgt ggtgacgcag gtgtttttca ccagaagttt tgtctacaga
    47701 caggctggtg actgcggtca gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt
    47761 tggtttattc tgttgtacgg ccatggacct tactcctcaa ttacggttta tccccgatcc
    47821 aacggggagc accaattact tacgctttaa gctggctaat acggcaaatg ggtttggctt
    47881 ttgcacttct tcaggcagtt caccaaagac catgtccgct tcggacactt cacagtgttc
    47941 agaatcatgc accggaacta ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag
    48001 cagatcgatt tcaccgaatt cgttaacctc aatcggttca tacgcttccg gcagtgcttc
    48061 agcctgttcg tctgaacgca caggactaaa acaatacgtt gtgtagacct gatgagtaaa
    48121 cggcttcccg caacgctgac actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac
    48181 tgcgagacgt tggttatcga tagcgaacga catggagcat tccacatcac tgtccacact
    48241 gactacggat tcggcgacgc gctcaacctg atcaggggta tagataccct ggtaatcaag
    48301 gcgtttttga gccgtacgaa ccggatcgag agtcaggggt aattttacct tttgcatagg
    48361 gcgcgcatat taactttgta acgtcataga gtcaaagaaa aaggcagcct gtggttgcct
    48421 tttgccaata attcgcacac attgcgggtt actgctttat tttcgttcag tgctgataac
    48481 gaacatcaac ctgcaaaacg aagcgcatag tttaaaatgg ctatcatcaa tacgctatat
    48541 ctgatggaaa aaaatatgcc taaacttatt ttagcctcca cctcgccatg gcgtcgcgct
    48601 cttctggaaa aactgcaaat ctcttttgaa tgtgcagcac cagaggtcga cgaaacccca
    48661 cgcagcgacg aatcaccgcg acagttggtg cttcgactgg cacaagaaaa agcgcaatct
    48721 ctggcgtcac gttatccgga tcatttaatt attggttcag accaggtatg tgttcttgat
    48781 ggcgaaatca ccggtaaacc gttaacggaa gaaaatgcct gtctgcaatt acgcaaagcc
    48841 agcggcaata tcgtcacctt ctatactgga ctggcgttgt ttaattcggc gaatgggcat
    48901 ctacaaacag aagtggagcc ttttgacgtc catttccgtc acctgagcga ggcggagatt
    48961 gataattacg tgcgtaagga gcatcccctg cactgcgcgg gtagctttaa gagtgaagga
    49021 tttggcatta cgctgtttga gcgtttagag ggacgtgacc ctaacacgct ggttggttta
    49081 ccgctaatcg cgctgtgtca gatgttacgt cgggaaggga aaaacccgct gatgggatag
    49141 ttgtttgcgc tggatgcggt tacgtcgcat ccggcaatcg gtgcaggcgg cctgatgcgg
    49201 cgtaaacgcc ttatcaggcc aaccgcccat tatcaggctt atatgcttgt attagcgcgc
    49261 gttacgcagc ttttgcaaac aacgcttcaa accttcatcc atcggcgctt cgatacgcat
    49321 cacctcaccg gtccccggat gggtaaactt caacgctgcg gcgtgcagga acagacgatt
    49381 taatcccgtg cctgcttcag tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc
    49441 aaaggcaatc ggatgacccg catactgtgt atgcacacgg atctgatgag tacgtccggt
    49501 taccgggcta caacgcacca gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt
    49561 ttccgacggt ttgccttcct gactgacacg cacgatacgt tcgccgcttt gcagaatatt
    49621 tttcaataac ggcgcttgaa cgctcttcac atgcgactgc cactgaccgc gcaccagcgc
    49681 caggtaatct ttttgcattc ctttttcacg taattgctca tgcagagaac gcaacgccga
    49741 gcgttttttc gctatcagca acacacctga agtgtcccgg tcgagacgat gaaccagttc
    49801 aaggaaccgc gcttccgggc gcaacgcccg caaaccttcg ataacgccga agcttaaacc
    49861 actgccgcca tgtaccgccg taccggaagg tttattcagc accaggatat gatcatcttc
    49921 atataagatg acgtccgcca gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc
    49981 ttcccgctca gcaacgcgaa ccggtgggat acgcacctca tcacccgctt cgagtttata
    50041 ttcaggctta atacgttttt tgttcacccg cacttcgcct ttacgcaaaa tacggtaaat
    50101 catacttttt ggtacgcctt tcaattgggt acgcaaaaag ttatcgatac gttgccccgc
    50161 ttcgtcagcg gtgatagcaa caatttttac ggatggagtc tctgttttca tggtgggcga
    50221 ttctaattag ccaacaggat tcgcgccact catttttcta tgcttatatt tagttttgca
    50281 ccttattact tcactacgtg atcactttat tgatggttat taaaccaatc accagcaaga
    50341 agtgaaaaaa ctgtgagtaa gcgggtgata aatggtaaaa gtcatcttgc tataacaagg
    50401 cttgcagtgg aataatgagg ccgtttccgt gtccatcctt gttaaaacaa gaaattttac
    50461 ggaataaccc attttgcccg accgatcatc cacgcagcaa tggcgtaaga cgtattgatc
    50521 tttcaggcag ttagcgggct gcgggttgca gtccttaccg gtagatggaa atatttctgg
    50581 agagtaatac ccagtctgtt tctttgataa ttgcgctgtt tttccgcatg aaaaacgggc
    50641 aaccgacact ctgcgcctct ttgagctgac gataaccgtg aggttggcga cgcgactaga
    50701 cacgaggcca tcggttcaca cccggaaagg cgttactttg cccgcagctt agtcgtcaat
    50761 gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat caacgcaact cagcaggaag
    50821 agttgcgcgt tgcccttgta gatgggcagc gtctttatga cctggatatc gaaagtccag
    50881 ggcacgagca gaaaaaggca aacatctaca aaggtaaaat cacccgcatt gaaccgagtc
    50941 tggaagctgc ttttgttgat tacggcgctg aacgtcacgg tttcctccca ctaaaagaaa
    51001 ttgcccgcga atatttccct gctaactaca gtgctcatgg tcgtcccaac attaaagatg
    51061 tgttgcgtga aggtcaggaa gtcattgttc agatcgataa agaagagcgc ggcaacaaag
    51121 gcgcggcatt aaccaccttt atcagtctgg cgggtagcta tctggttctg atgccaaata
    51181 acccgcgcgc aggtggcatt tctcgccgta tcgaaggcga tgaccgtacc gaattaaaag
    51241 aagcactggc aagccttgaa ctgccggaag gcatggggct tatcgtgcgc accgctggcg
    51301 taggcaaatc tgctgaggcg ctgcaatggg atttaagctt ccgcctgaaa cactgggaag
    51361 ccatcaaaaa agccgctgaa agccgcccgg ctccgttcct gattcatcag gagagcaacg
    51421 taatcgttcg cgcattccgc gattacttac gtcaggacat cggcgaaatc cttatcgata
    51481 acccgaaagt gctcgaactg gcacgtcagc atatcgctgc attaggtcgc ccggatttca
    51541 gcagcaaaat caaactgtat accggcgaga tcccgctgtt cagccactac cagattgagt
    51601 cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc gtccggtggt tcgattgtta
    51661 tcgacagcac cgaagcgtta acggccatcg acatcaactc cgcacgcgcg acccgcggcg
    51721 gcgatatcga agaaaccgcg tttaacacca acctcgaagc tgccgatgag attgctcgtc
    51781 agctgcgcct gcgtgacctc ggcggcctga ttgttatcga cttcatcgac atgacgcctg
    51841 tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc ggtgcgtcag gaccgtgcgc
    51901 gtattcaaat cagccatatt tctcgctttg gcctgctgga aatgtcccgc cagcgcctga
    51961 gcccatcact gggtgaatcc agccatcacg tctgcccgcg ctgctccggt accggtaccg
    52021 tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgttt gatcgaagaa gaagcgctga
    52081 aagagaacac ccaggaagtt cacgccattg ttcctgtgcc aatcgcttct tatctgctga
    52141 atgaaaaacg ttctgcggtg aatgcgattg aaacgcgtca ggacggtgtt cgctgcgtga
    52201 ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt gttgcgcgtg cgcaaagggg
    52261 aagaaacgcc gaccttaagc tacatgctgc cgaagctgca tgaagaagcg atggcgctgc
    52321 cgtctgaaga agagttcgct gaacgtaagc gtcctgaaca acctgcactg gcaacctttg
    52381 ccatgccgga tgtgccgcca gcgccaaccc cagctgaacc tgccgcgact gtcgtagccc
    52441 cagcaccgaa agctgcaacg gcaacaccag cagctcctgc acaacctggg ctgttaagcc
    52501 gcttcttcgg cgcactgaaa gcgctgttca gcggtggtga agaagccaaa ccgaccgagc
    52561 aaccaacacc gaaagcagaa gcgaaaccgg aacgtcaaca ggatcgtcgc aaacctcgtc
    52621 agagcaaccg ccgtgaccgt aatgagcgcc gcgacacccg tagtgaacgt actgaaggca
    52681 gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc acagcagcag actgccgaga
    52741 cgcgtgagag ccgtcagcag gctgaggtaa cggaaaaagc acgtaccacc gacgagcagc
    52801 aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga taaacgtcag gcgcaacaag
    52861 aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga aaccgaacag gaagaacgtg
    52921 tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa tcagaaagtg cgttacgagc
    52981 aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga agaaactgcc gctgccgaac
    53041 caattgttca ggaagctcca gctccacgca cagaactggt gaaagtcccg ctgccagtcg
    53101 tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc tgataaccgt gacaacggtg
    53161 gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg cgtgagtggt cagcgtcgtc
    53221 gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat gccgttgacc gtagcgtgcg
    53281 cgtctccgga actggcctct ggcaaagtct ggatccgcta tccaattgta cgtccgcaag
    53341 atgtacaagt tgaagagcag cgcgaacagg aagaagtaca agtgcagccg atggtgactg
    53401 gggtccctgt cgccgccgct gtcgaacctg ttgttagcgc accggttgtt gaagaagtgg
    53461 ctgaagttgt agaagccccc gttcagattg ccgaaccgca accggaagtg gttgaaacga
    53521 cgcatcctga agtgattgcc gccgcagtaa ctgaacagcc gcaggtgatt accgagtctg
    53581 atgttgccgt agcccaggaa gttgcagaac acgcagaacc ggtggttgaa ccgcaggaag
    53641 agacggcaga tattgaagaa gttgccgaaa ctgctgaggt tgtggttgct gaacctgaag
    53701 ttgttgctca acctgccgcg ccagtcgtcg ctgaagtcgc agcagaagtt gaaacggtag
    53761 ctgcggttga acctgaggtc accgttgagc ataaccacgc tacggtgccg atgacgcgcg
    53821 ctccggcacc ggaatatgtt ccggatgcac cgcgtcacag tgactggcag cgccctactt
    53881 ttgccttcga aggtaaaggt gccgcaggtg gtcatacggc aacacatcat gcctctgccg
    53941 ctcctgcgcg tccgcaacct gttgagtaat aattagctca agtaatcaag ccctggtaac
    54001 tgccagggct tttttatttc atctttgaat caacatatta aaattcatcg aatggcatcc
    54061 ttgctaacca acaatgcaaa ataggcaaaa aaatgcccgc cccggcgatc ccagagcagg
    54121 cagacaaaac atacccagtt tcatgatatg ttttaaagcg aaattatttg ttgagctgga
    54181 agagcgacaa tccctgcata tcggtaaatg ctttatacga tgcctgcaat gccgtttgct
    54241 gcatgatgta agatgaaata gttgcattcc agtccacatc aaccagatcg ctcatctgct
    54301 gcgtttgccc taaagcgcga tcgctaccta atgaatccag cgactccagt tcgttcagct
    54361 gcgtgcctaa ttccgcgcga acggtcagca cattgttcag cgagtttttc agtccgcggt
    54421 tggttttatc taacgccgca gcggcggttt ctttatccgc ttcgctatcc gcgaccggcg
    54481 ttttcagggc tgcgatggcg ctatccagca tggcaaaaag attggtttca gaagcgctac
    54541 cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa aattttgtcg cccgtatgtc
    54601 ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat actttctgtt ccgccgacat
    54661 aatcaccgtt agcctcacta aacggcgcag tctctgtttt ataaccggca aaaatgtagc
    54721 gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc ccctgaatat
    54781 ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg tagacaattt
    54841 tttcctgagc attctggata gcagtggtga cctggctaag tacactctct tccagtgaca
    54901 ctttttgagt ggcgaaagta cgcgccagcg tgtactggct gttttgcgcc tgtgcctggg
    54961 agagaacaac cgcttgtgat gcagcaatgg gatcatctga agggttaacg actcgcttac
    55021 ccgtcgacat ctgttcgccg tacttcatcc attctgcctg agaattggtg ataccacgca
    55081 tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt agcgaatgtt
    55141 aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg ccaggtaata
    55201 ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg aaatcgactg
    55261 ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg ttttcaacgt
    55321 cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa aggatttcgc
    55381 accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt tgcggttatc
    55441 gctatcaccc gcatcttttt cgctcgccat cgctattttc gcttcgtcgg tgattaatac
    55501 atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt taacggcagg
    55561 cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg catccggcgt
    55621 caccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat tatcgaacga
    55681 gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac cgatcgcaac
    55741 gtcacctttg tttttcgtgt tttgcagaac cgcgggctta ccgatagtaa agaaatcttc
    55801 accagcatcg ccgttagcat caaatccggc tttgtgttgg gtgttgaaag cctcggcaaa
    55861 tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agctcctgag aacggaatgt
    55921 cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa tattgcctgc
    55981 cgtcccatca acataagcga cagtcgtacg agaagggtca gcgctggaag gaactgccgc
    56041 cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggccaccg tgatgttata
    56101 agtgccgcca tcctgaacgc tgacttctac accaacaatc tggtttaatt cgctcaccag
    56161 ttgatcgcgt tgatccagca gattgttagg tgacgcccct gcccccacgc ctgtcaggcg
    56221 cgagatttga tcgttcaggc tggcaatttg tttagcgtaa ttgttgatct gatcaacgct
    56281 ggcacctatc gcgatattga cctgtttgtc ctggtcgcgc agatattgat cggtggtttt
    56341 aaactgattc accaaccctt ctgatttccc aatcagcgcc tggcgcgctg ccgggtcttc
    56401 cgcgttactc accagcgttt gcaggctggt gaagaaatcc tgcatctgtg ttgccagcga
    56461 agaggtactg gtggagagca tattgtcgat tttcgacatc tgctcatagc gggcggtcag
    56521 gccgctactt tgcgtctgcg cagcacgtaa ctggttggta ataaacgcat catactcacg
    56581 ctgcacacca gaaacgtaga cgccattgcc aacccagccg ccagcgccca acgtgctatt
    56641 ggcctgcgcc ataatagtgg tttggcgggt atatccggca acgttatagc tggagatatt
    56701 attacttgcc gtatttaacg ccgcctgggc cgcgttcagt ccgctcatgg cgttattgat
    56761 caagctggac atggaggttc cttataagcc ttcaattaca gagtattatc ggcagcgacc
    56821 cgccggactt gagttattca gaacagatta tcaatgttca tgctgtaggt tttgctcacc
    56881 ttgtcgctta tcgatttcat ctgctggatc atgttggtga gtttacgggc atagtgagga
    56941 tcggtagcat accccgcgtc ctgtagggcc tgcgccccct gctccgcact cgcggctgtc
    57001 gtcacggcgg cgtagcgcgg gttacgtgtt aacagcccaa cgtaatccga caatgcttcc
    57061 agatacgagc tgtagacccg aaatttcgct tttactttct tcgcttcgcc attttcatat
    57121 tcagtcgtgg tgatttcagt aactggacct ttccagttgc cagaggcttt gacgccaaac
    57181 aggttatagc tcggctcgcc gttttcgcgg cggatttgcc gttgccccca accagactcc
    57241 agcgccgcct gagcgaggat caaatgatgt ggcacaccgc tttgctggct tgccagttgg
    57301 gcgggcagcg agagttgcgc gaggaatgct ttactgtcac ccggcagcga atcatcgtag
    57361 ttacgtggca cggccttttg caccagctgc gaaagcgcct gattttgata acgcaccaca
    57421 gtttcgagcg ggaatttcat cggtgctgct ggcgtggact cctctggcaa tggttgttct
    57481 ggcgtcatct gtttaaccat catctctgca agccccagac ctttgcccgc cgtcatctgt
    57541 tgggcaatct gctggtcata catactggta tacaggcgag tgtgctcgct gctgaacagg
    57601 ccatcttttg gtaaagcgtc gcgcatgctt ttcaacatca tctgcacgaa catcccttcc
    57661 acctgacggg ccaccggacg gatatttgcc gccggatctt cgcccgcttt cgcctttagt
    57721 tcgttgagtg attgcgcatc ccaggccgca cttgccagta gtttgctgtc gctgatcatc
    57781 agatgatttc cagttttgcc cgcagacatc ccgcactttg cattgattgc agtatggaca
    57841 tcagatccat cggcgtagcg cccagcgcat tgagcgcgcg caccacgtta ttgaggctgg
    57901 cgctggaacg tacgctttgc agcgaaccgc cgctctggcg taaatcgatc tgcgtttgtg
    57961 gagtaaccac agtctgtcca ccaccaaacg gtgtatctgg ctggctgaca ttggcctgac
    58021 gattgactgt tactgagaga tttccctgcg ctaccgcgca gctgtcgagg gtcacttcgc
    58081 gattcatcac caccgaaccg gtgcgcgagt taatcactac tttagcgtcc tgcggggtga
    58141 cattaacctg catattctgg atatctgcaa ggaaacggac ctgggaactg ttgccacttg
    58201 gtacgcgcac ctgaatagtc cgcgcatcta acgcggtggc gctgccatat ccacgcacgc
    58261 ggttgatggt gtcagcgatt tgctgcgcca tgctgaaatc ttcgtcgtta agttgcaaat
    58321 taagggtatt cccgacgcca aactggctgg gcaattcacg ttcaataacc gcaccattgg
    58381 tgatccgtcc accgttcagt tggttcacct gaacactgct accgccagcg gaggctcctg
    58441 cgccgccaac cagaatattg ccctgcgcca gcgcatacac ctgactgtca acgcccttaa
    58501 gcggcgtcat caacaacgta cctccacgca agcttttggc atttcccatg gaagaaacca
    58561 ccacatcgat ggtttgcccc tgacgtccaa acggaggaag tgactctgtc accattaccg
    58621 cagcgacgtt ttttagctgc atattggtgc ccgtcggaac ggtaattccc agctgtgaga
    58681 gcatgttatt aagcgtttgt gtggtaaacg gcgtctgggt tgtctggtca ccggtgccat
    58741 ccagccccac caccagacca tagccaatca gtgagttttg ccttaccccc tgaacactgg
    58801 tgagatcgcg aatacgctca gcctgagccg ccgtcgtaac cagtagaaga attaatgcag
    58861 agagaaattt aatcaccaca gcctcactta cattggcgac aggttaagga agaaacgctg
    58921 caaccagccc atattttgcg cttcgttaat gtagccattg cctacgtatt caatgcgcgc
    58981 atccgccacc tgagtagacg gtacggtatt gctgccgctg atagtgcgtg gattaaccac
    59041 gccagagaag cgaataaatt cggtaccctg attaatggca atctgttttt cacccaccac
    59101 atgcaggttg ccgttgacca gtacctggtc aaccgtcacc gtcaacgtgc cgctaaaggt
    59161 attgctggca ttggccccgc cctttccgtt gaacgtgtta ccaccggagg cttcgacatc
    59221 ggcacgagcg ttaccaaaca gcccctgcaa atagcgcggc acagtatcaa agccaaaatt
    59281 agttttaccg tcacggctgg cattcgcaga ggagcttttg ctggcgctga cgttctcctg
    59341 caacacgatg gtcagcgtat cgccaatatt gcgtggtcga cgatcttcaa acagcggttg
    59401 atagccatag ttaatcggct gagcagactg gaaaatagaa ccgttggcga cgggcgtcgg
    59461 accgggaacc ggttgtgcac tggtcgcccc ctgcaccagc ggcgtggagg gtatccaggc
    59521 gcagccggtt agtgaaagca ccaacaagct ggaaatggca taagtatgcg cagcgttttt
    59581 ttgcatggct atcatcttca aaaatcagta aaccggtgaa cctgccaccg gttaagcctt
    59641 agagttgcgt cagtttttgc agcatctgat cggtggtgga caccgcttta ctgttgattt
    59701 cgtaagcgcg ttgcacctga atcatattga ccagttcttc cgccacgttg acgttagacg
    59761 tttcaacata cccttgatac agcagtcccg cgccgttcag gcccggcgtg ctttcgttcg
    59821 gtacaccaga ggattgcgtt tcggtgtaga ggttttcgcc aatgctctcc agcccggtgt
    59881 cattcataaa ggtggtgaga ttgagctgcc caacctgaac cggagctgcc tggccttgtt
    59941 gggttacgct gaccacgcca tcacgaccga tggtgatact taacgcattc gccggaatgg
    60001 tgatcgctgg ctgcacctga aaaccaccag ctgcccgttc tgatccacct ggaaagagcc
    60061 gtcacgggta taggctgatg aaccatctgg caacatcacc tggaaaaagc cctgcccttt
    60121 aatcgcgaca tctttgctgt tgttggtctg cgacaggttt ccctggctgt gtaagcgttc
    60181 agtggcgacc gggcgtaccc ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc
    60241 ggaagactgt gcccccggct ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg
    60301 ctgacgctta aaaccgttag tactgacgtt tgccaggttg ttggcaatga cgtccatatt
    60361 ggtttgctgg gcgtcaaggc ccgttttggc gatccataat gaactgatca taaaatgtcc
    60421 tgtagtggat tagtgacaaa gtgcatatgt cttcttgccg gatgcggcgt aaacgcctta
    60481 tccggcctac aagtgctcgc aaaatcgata aattgcgatt gcccccatag acccgataaa
    60541 cttgcgcatc gggcaactta tacttgtcat gtatcctttc aattaactca tcgacagcag
    60601 ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa
    60661 acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc
    60721 cagaaccccc gacatcacac gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc
    60781 ctgggtttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt
    60841 ggctttcacc agtttcagac gccctactgg cgcaactgta tttgccggat cgcccggatt
    60901 gagcgccgag attgtgccat cggcagcaat agtgatttcc gctccttccg gcacagcaat
    60961 tggccccgcc tcgcctatca cctgatgccc ctgaattgtc agttgcccgg tgggatcaac
    61021 ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca
    61081 gccatcctgc tgcaacgcga cgtccagcgg gcgcgaggtg taatccattt tgccgggcgt
    61141 catatctgcg cccggcgttg acgccgtgac caacgtgcgc gtgggcagag aaagcccttc
    61201 cactggcacc gcgcgtaaag cattcaactg cgcgcgaaaa ccgggcgttg aggcattggc
    61261 cagattactg gcggttaccg cctgttgatt cagtgtctgg ctggctgctc ccatcgcggt
    61321 ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta accagcgtgt
    61381 tgaggatctg gtcctgagtt ttgatggtct gggcgttaga ctgatagtta cgctgggcaa
    61441 cgatcatatt gaccagttct ttactgagat cgacattgga cgcttccaac gcaccgttgg
    61501 tcagggtgcc aaagttgccc gtcccggctg tccccaacag cgccacgcca gaagattgcg
    61561 tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca aagttcgcca
    61621 gtacaatctg ccccagcagt tgtgtttgtt cgttggaata gttgccgaca accgtaccgt
    61681 catcattgat ttgataactc accagatcgc ccggtttgta gccattctgg gtggttgcca
    61741 caatattgtt agcgccggta ttttgctgca tggagttgag gaagctcagg ctaaacgtgg
    61801 cgggatctgc gccgttaatt gcgccagtgg taatattctc tgttggattt gaggtcagaa
    61861 cgccattggc attaaacacc agcttcattg caggctcggc tgtacctgtt ggatcactgc
    61921 tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaagtag acgctcatgt
    61981 catgagcatt accctgactg tcgaaaacag tcaccgaacc ttttttgtta tagctatccg
    62041 cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc aggttgatct
    62101 gcatcgacgc cgtggtggta gttttcgctg ccatcagggt attcgggatc gaaatattgg
    62161 tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa cccgtcagct
    62221 gtaaaccttg catattcacc aggttacggt tttcatccag cttaaattgt ccgttacggc
    62281 tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accgttctgg ctgatagcaa
    62341 cgtccagacc tcgcccggtg ttggtggtcg tgccatcggt aaagtcctga gtgataccgg
    62401 caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa gaggccgtgc
    62461 ctgatttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca tcgaggttgg
    62521 tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac tcctgaaatg
    62581 tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa gtgccgagat
    62641 ccagcgtatt accgctgttg ccgcggatca caccctgcac cagagcaaac tgcagcggct
    62701 gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa gaaccgttcg
    62761 gcgcagtgct gccatcagtc aacgtaccgt cccaggtgaa actgtggact ccggcggtca
    62821 gttcaccaat atcaatggtg cgcacaaccg cgccattttt atcggtgatg gtggccgtaa
    62881 ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc acagcccctt
    62941 cttcactgcc ggttcccgca agaacagtgg tgccggggat catcacgccg tgaccgatca
    63001 ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat ccgagcgtgg
    63061 tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac gtcagctcgt
    63121 tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccaac aaagtcagaa
    63181 aactgctttg taagtctgcg gcgttgctgc ccgtgagcga actactgctg gtggtactga
    63241 cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc tcctttattg
    63301 accgagcgta agggttttca gcatcatgct tttcaccatg ttgagcactt caacattggc
    63361 ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat caacgttcgg
    63421 catttttacg tagccctttg catccgccag cggattaccc ggttcataaa ccagtttgtc
    63481 cggggcctga ctttctataa catcggcaac ctttacgccg cctgtcgcag cacctggtgc
    63541 agcgttaacc tggaatacca cctgttttgc ccgatatggc tgtccatcgg gaccggtcac
    63601 gctatcagca ttcgccagat tactggccgc cacgttcaag cgctgggact gggcagttaa
    63661 cgccgacccg gcgatatcaa aaatattcag cagtgccatc cgttaatttc cgctctgtaa
    63721 aacgttcatc atgcctttga tttgcccgct caacgcgcta aggctcatct ggtattgcag
    63781 gctgttatcg gcaaactggg tgcgttcgcg atccatatcg acggtattac cgtcaagcga
    63841 aggctggtcc ggaatacggt attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg
    63901 aatgtgttgc gttgaggtca tcgtcagtgc aaccacactg gttgcatccc gtccacgttg
    63961 catgactttt ttaagttcac tggcaaaatc gatatcgcgc gcctgataac caggggtatc
    64021 ggcattggcg atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc
    64081 ttgttgaaaa cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc aggtatcaaa
    64141 attctgccat cagcttaaat gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg
    64201 ggtcgctatt tatgccgttg atgaccattg cggacaggta caattcacgt tgtagaaatg
    64261 gctgggggcg aaaatgctgg caataaaacg tagcgtggcg atcatctcga tactattcac
    64321 tccgttaagt gcggcgagta atctcacatc gcaattgcaa acctttttta gcgcccagct
    64381 cgcgggggta agtgatgagg ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc
    64441 gtgcgagcag ccattgcttt cgatgagcaa taattcccgc ctgtggggca atgtgaatgt
    64501 gttggcacgc tgcggtaacg acaaacgata tttacaggtt aatgtacagg ccacaggaaa
    64561 ttatgtggtt gccgcgatgc ccattgtgcg gggaggaaag ctggaagctg gcaatgtcaa
    64621 actaaaacgc ggacggcagg ataccctgcc accgcgtacg gtgctggata tcaatcaact
    64681 tgttgatgcc gttagcctgc gcgatctatc acccgatcaa cctatccagt taacccagtt
    64741 tcgccaggca tggcgggtaa aagcgggaca acgcgtcaac gtgatcgcca gcggtaacgg
    64801 gtttagcgcc aacgcagaag gtcaggcgct gaacaatgca gccgtcacac agaatgcgcg
    64861 ggtgcgcatg gtatcgggac tggtggtcag cggcgttgtt gatgcagatg ggaatattct
    64921 tataaaccag taagctgtta aagattaccc gtcccttgcc gataaataag caacacatga
    64981 taaaagcgcc ctcaatgagg aataaaccat gagtattgat cgcacttcgc ctctgaagcc
    65041 cgtaagcacc gttcaaccac gcgaaaccac tgacgcgtcg gtaacgaaca cccgggcggc
    65101 aaaaacaaca gcctccacca gcaccagtgt gacgttaagc gacgcgcaag caaaactgat
    65161 gcaacccggc agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg
    65221 taacggtgaa ctaaaaatgg acaccggcaa aattgccgat gcgctgatca acgaagcgca
    65281 gcaagacttg cagagtaact gatcgtatga cacgtcttgc agagatcctc gaccagatgt
    65341 ccgctgtgct taacgatctc aaaacggtaa tggatcaaga acagcaacat ctctctatgg
    65401 ggcagatcaa cggcagccag ttgcaatgga ttacagaaca aaaaagctca ctgctggcga
    65461 cgctggatta cctcgaacag ttacgcagga aagaacccaa tacagcaaat agcgttgata
    65521 ttagtcaacg ctggcaggaa attactgtga aaacgcagca actacgccaa atgaatcaac
    65581 ataacggctg gttactggaa ggacagattg agcgcaatca acaggcgctg gaaatgttga
    65641 aaccgcatca ggaaccgacg ctatatgggg cgaacggtca gacctcaaca acccatcgcg
    65701 gcggtaaaaa gatttcgatc tgaatattat ctccggcctg cactgcaggc cggaatgcat
    65761 tgttacaccg tccggcgggc aaattcttta actttgaagc ccagtaccgc cagtgcagcg
    65821 aagtacgcgg caatccccgc cagcacgacc gccattaaac gcagtaagcg ccaaggcatg
    65881 gtacccaatg accactccgg catgatatgt aacataccta aaagcacgcc agacatcacc
    65941 agtaccgcca ccaccagacg caacagaaac gccatccagc cgggttgcgg agtaaagatt
    66001 ttctgcttac gcaactgcca gtaaagcagc gaagcattca gacacgccgc cagaccaata
    66061 gaaagtgaca gcccggcatg tttcaacgga ccaataaacg ccaggttcat caattgcgtt
    66121 aaaatcagcg taacgatggc aattttcact ggcgttttaa tgtcctggcg ggaataaaag
    66181 ccaggagcca acactttcac tacaatcagg ccaatcaaac ccaccgagta ggcaattaac
    66241 gcccgctggg tcatcagcgc atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg
    66301 gttaacggac ccgaaagaat gcccaacgca accgcactcg gcagcgccaa caggaaacaa
    66361 agacgcaacc cccagtccat caaacggttg tattcatcat gattgccact ggcaaaactt
    66421 ttcgacagcg acggcagcaa aatggtgcca agcgccacgc ccagcacacc ggagggaaac
    66481 tccattaagc ggtcggcgta atacatccaa gacaccgaac cggaagcaag aaacgaggca
    66541 aaaatggtgt tgatgattaa ggagatctgg ctcacagaga cgccaaggat cgccggtccc
    66601 atctgtttca ccacgcgcat tgctccggca tcgtggaagt taatgcgcgg caggaccagc
    66661 atgccgatct tcttcaggtg cggtagctga tacaccagct gcaggatgcc gccgaccgta
    66721 acagcccacg ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa
    66781 ccaatcatgc tgatgttaag cagtgttggc gcaaacgccg gaatcgagaa gcggttccac
    66841 gtattcagaa tcgctcccac cagcgacgcc agggagatca gcaagatata gggaaaggta
    66901 atctttagta gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg
    66961 gtcaccatga tcacccacgg tgcggcgagc atgccagcga ccgtcacaac cgccagcgca
    67021 agtgtcagca ggccagaaac ataagagaca aagacccgcg tggcgtcttc accctgctta
    67081 cttttatatt ccgccagaat cggtacaaat gccagggaaa atgccccttc ggcaaagata
    67141 cggcgtaaca agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg
    67201 ccaaagattc tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc
    67261 atcgagctga cggcggccag cgattttaat aaattcatcg gtgttctaat ccagacccac
    67321 gggcaaaacg cctgcaaggc aggcgctgaa agaggcgaat agtctacctg gattatggtg
    67381 aattgctacc gccagatgtt acagggttta ttcactcatc gcatcgcgcc agatcttgtc
    67441 aacgatacgt tgcgccagca ctgcctgttc gccggcggtt tgcagaactg tctgattttg
    67501 cacacattca atgaagtgcc gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg
    67561 ccaaccagga atcggtttat gcactacgcc ctgcccgcgc tcctcacgcc attcgcgcat
    67621 atccgtaatg tcgatgagcg caccgtcagt cacggcctgc acggtttcac gctgacttcc
    67681 ggcacggcga tgcatacagg tggtgatctg caaaggatcg gccgaaaaat ggtgctcggc
    67741 aaacagcatt tcgccagcgt cgttagtcag tagcgtaccg ccatccagag aggctttgcc
    67801 gcccgacaac cacagcgcgg tatccaccac atgcagataa tcatccagca acgtgaaata
    67861 aagatcgtgt ggcccgacac tattgctacg atgtttatcc attcttagcg aagctgcggt
    67921 agcgagttgc gtttttaact caccgtagag tggtgcgaaa cgacggttaa aaccgaccat
    67981 caacgtcagt tttttccgcg ccgccagttc caccagccgt tcagcatcgc gcagattttc
    68041 tgccagcggt ttatcgacac agacatgtac acccgcattg agtaacgtac tgaccacgtc
    68101 aaagtggctg gcggtgctgg aatgcacaaa aaccgcatcg caactggcgg caaggctgga
    68161 taacgaatcg gcataaggaa tgcgccagct ttcacaaatt ggcagggctt tcgcgcgcgt
    68221 aggcgaccag gctccttgta acatccagtc agacgctgcc gccagcaccg gtaaccacgc
    68281 tttttgcgca atgccgccta atcccacaac gccgatacgt aattttttca ctttaatctc
    68341 ccagatgggc cagcaacgaa tcaagacgct gtttcagttc tgccacttcg atttccaggg
    68401 cttcgacgcg ggcctgtaaa tcaccatcaa ccgcgttcga catatccgtt accgccggct
    68461 gatcctctac ctcaccactg aaaagatgca tgtagcggct ttcgcgttta cccggttcgc
    68521 gggccagacg caccacaaaa ggaccatctt cgcgatttgc cagttgttcc agcgtcgact
    68581 ccacttccgc catatcgctg aattcataca ttcgcgcggc gcggctgcgc agttcacccg
    68641 gcgtctgggc accacgcaat aacaacgtgg tgattaacgc cacttccgct gcgctcagtt
    68701 tcagatcgcc aaattctgaa ttacaaaaac gttgttcata tttggtgacc cgattaccaa
    68761 agccactcac cgtgcgcaga taatggcgtt tgaccagatt atccagttgc tcctgcactt
    68821 cagattcact cagattcatg accggttcac ggttcgtttt ctgattacag gccgtgacta
    68881 caccattgac tgagagcggg tattgctccg gcgtggtcac ctgcttttcc agcaagcaac
    68941 caatcacgcg agcttcaagg gcagtaagtt gatatttcat gagattctcc ttagcggccg
    69001 ggcgtccagt ctggggtagt taatgccgtc agtacgtgat cgcgccattg tccatcaatc
    69061 aacagatagt ctttcgcata gccttctttt tcaaaaccca gtcgcgccag taaatcaccg
    69121 ctgcgtttat tgtgcggcat ataattagcc ataatgcgat gaatatgttg ggtgcgctgc
    69181 atataacgaa tggctgcggt cagggcttca aacatgagtc ctttgccctg ccatttttgc
    69241 ccaatcgaat aaccgagata gcaggcatga aaagagccac gaacaacatt ggaaaaattg
    69301 gcaacgccaa taatctcttt ttcgtccggg tcgaataagc caaagtagaa agctgaacct
    69361 tgtttatgaa attcgttaat catccccagc ctggcctgcc agcctgatgg ataacagtgg
    69421 ctttcgtcgc gcactggctc ccagggcttg aggaaatggc gattctctgc gtaataatcc
    69481 gcaagacgcc aggcatcacg atcatgcacc agacgcacga ccagtcggtc tgtggttaag
    69541 cgcacttttg gcacgttact gcgatagcca aacattctat acctactcct tcccgtaacg
    69601 tctttaatac gctgtaagta ttactatacc tgcgcaacca gaccctgtga aaagtccaaa
    69661 atgccatttt ctgcattatt cacatatttg ataaaaactc tctatcaaag cgacgttttg
    69721 ataaaaaaat attgtccccg aagtgatggg aaaagtctta gcggcaatgc agaatagaaa
    69781 gtgctcatct tttatttccc gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg
    69841 ggtaaatatt tcctgctcat cgataatatg ctggtcgtgc tggggttctt tgttgtcttc
    69901 ccgctgatct ctatccgctt cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt
    69961 gctctcggtc tacgccagtt tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc
    70021 gaccgctttg gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc cgccggattc
    70081 gccacaatgg gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct gctctcggga
    70141 ctcggcggca cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca
    70201 caacaacgtt gtcgtttttt ctcgctgttg atgatgcagg acagtgccgg tgcggtcatt
    70261 ggcgcattgt tggggagctg gctgttgcaa tacgactttc gcctggtctg cgccacaggg
    70321 gccgttttgt ttgtgctgtg tgcggcgttc aatgcgtggt tgttaccggc atggaaactc
    70381 tccaccgtac gcacgcccgt tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt
    70441 gtcacctatg tcctgacgct ggcgggttac tacatgctgg ctgtacaagt gatgctgatg
    70501 ctgccaatta tggtcaacga cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc
    70561 attgaagcgt gtctgtcgtt aacgttgctc taccctatcg cccgctggag tgaaaagcat
    70621 tttcgtctgg aacaccggtt gatggctggg ctgttgataa tgtcattaag catgatgccg
    70681 gtgggcatgg tcagcggtct gcaacaactt ttcaccctga tttgtctgtt ttatatcggg
    70741 tcgatcattg ccgagcctgc gcgtgaaacc ttaagtgctt tgctggcgga cgcaagagct
    70801 cgcggcagct atatggggtt tagccgtctg ggtctggcga ttggcggcgc tattggttat
    70861 atcggtggtg gctggctgtt tgacctgggc aaatcggcgc accagccaga gcttccgtgg
    70921 atgatgctgg gaattattgg catcttcact ttccttgcgc tgggttggca gtttagtcag
    70981 aaacgcgcaa cgcgtcgttt gctggaacgc gacgcctgat ttgatactcc cccctgactc
    71041 ctccatactg aaagtgaact gacagccaaa tggaggagtc acgtgaagct atacatttac
    71101 gatcactgcc cttactgcct caaagcccgc atgattttcg gcctgaaaaa tatccccgtc
    71161 gaattacatg ttctgctcaa cgacgacgca gaaacaccca cccggatggt cggtcaaaaa
    71221 caggttccca ttctgcaaaa agatgacagc cgctatatgc cagaaagcat ggatatcgtt
    71281 cactatgtcg ataaactcga cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt
    71341 gaagagtggc tgcgcaaggt caatggctac gccaacaaac tgctgttgcc gcgttttgcc
    71401 aaatcggcat ttgatgagtt ttctactccc gccgcgcgca aatatttcgt cgacaagaaa
    71461 gaggccagcg cgggtaattt tgccgacctg ctggcccact ctgacggtct gattaagaat
    71521 atcagcgatg atttgcgtgc gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc
    71581 gaactttcgg aagatgatat tcagctattt ccgctactgc gtaatctgac gctggtagcc
    71641 ggaatcaact ggccaagccg cgttgctgat taccgcgata atatggcgaa acagacacaa
    71701 atcaatttgt tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc
    71761 gggttgccgc cgttttcagt tttttctgct ggcgagttta tcgctggtga cacatgctgc
    71821 gttgtgtcag gatattgacg cagatttcat gtcgaggact accatgaaca agtttttatt
    71881 tgccgctgca ttgatcgtca gtggcctgct cgttggctgt aatcaactca cccaatacac
    71941 catcaccgaa caagaaatta accagtcgct tgcgaaacat aataatttct caaaagatat
    72001 cggtttaccc ggcgtggctg acgcccatat tgttctgaca aacttgatca gccaaattgg
    72061 tcgcgaagag ccgaataagg ttaccctaac cggagacgcc aatctggaca tgaactccct
    72121 gttcggtagt cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa
    72181 agaaaaaggt gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga
    72241 aaaaatgcaa acggtgatgc aaacgttgct tccctatttg aaccaggcat tacgcaatta
    72301 ctttaaccag caacctgctt acgtcctgcg cgaagatggc agtcagggcg aagcaatggc
    72361 gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga
    72421 ttaatgacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt
    72481 atcctttgtg tccggcaaaa acattccttc agccggagca tagagattaa tgactgcacc
    72541 atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc gcgatggcga
    72601 catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta tcgtaatgcc
    72661 caatctggct ccgcccgtga ccacagttga ggctgccgtg gcgtatcgcc aacgtattct
    72721 tgacgccgta cctgccgggc acgatttcac cccattgata acctgttatt taacagattc
    72781 gctggatcct aatgagctgg agcgcggatt taatgaaggc gtgttcaccg ctgcaaaact
    72841 ttacccggca aacgcaacca ctaactccag ccacggcgtg acgtcaattg acgcaatcat
    72901 gccggtactt gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac
    72961 acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc
    73021 tctgcgccag cgcctgactg cgctgaaagt cgtttttgag cacatcacca ccaaagatgc
    73081 tgccgactat gtccgtgacg gaaatgaacg gctggctgcc accatcactc cgcagcatct
    73141 gatgtttaac cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc
    73201 catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctggtcgcca gcggttttaa
    73261 tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag agagcagttg
    73321 cggctgcgcg ggctgcttca acgccccaac cgcgctgggc agttacgcta ccgtctttga
    73381 agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc cgcagttcta
    73441 tgggttgccg gtcaacgaca cattcatcga actggtacgt gaagagcaac aggttgctga
    73501 aagcatcgca ctgactgatg acaccctggt gccattcctc gccggggaaa cggtacgctg
    73561 gtccgttaaa caataaaaat cgtagcttcc tgttgtcatt aggttattta acctgtataa
    73621 ataaccagta tattcaacag ggggctatta tgcgaattga agtcaccata gcgaaaactt
    73681 ctccattgcc agctggggct attgacgccc tggctggcga actttcccgc cgtattcagt
    73741 atgcgtttcc tgataatgaa ggccacgtat cggtacgtta tgccgcagcg aataatttat
    73801 cggttattgg cgcaacaaaa gaagataaac agcgcattag cgaaattctc caggaaacgt
    73861 gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg
    73921 ggtcgccccg gcttttttat tttacttacg aattttgagt acaaaatttc tacacttcct
    73981 caaaataatt gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat
    74041 aaatcttaaa agcagaaatt tatctgttcc cccctttaaa aattcggtga atacccttac
    74101 ttattggtat actgaagttg cttcagataa aaacgcattg aacctcgaat aacgttgtct
    74161 agtaacacga attagggggc catgatggaa aaaaataatg aagtcattca gactcatccg
    74221 ctcgtagggt gggacatcag caccgttgat agctatgatg cgctgatgtt gcgtttgcac
    74281 taccagaccc caaataagtc cgagcaggaa gggactgaag ttggtcaaac gctctggtta
    74341 accactgatg ttgccagaca atttatttcg atattagaag caggaatcgc caaaattgaa
    74401 tccggtgatt tccaggtaaa cgagtatcgg cgtcattaac atgacatgac catccaccca
    74461 aaaggcacct caccggtgcc tttaccattt ctgggttgta tatctgcatt cagttaatta
    74521 ctctgcttaa accatgagag cagagagaaa aaaatgaaat acgatctcat cattattggc
    74581 agcggttccg taggcgctgc cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta
    74641 atgaccgacg cccatatgcc accgcatcaa cacggcagcc accacggcga tacgcgatta
    74701 attcgccatg cttatggtga aggcgaaaag tatgtcccgc tggtcctccg cgcgcaaacg
    74761 ctgtgggatg aactttcccg ccacaacgaa gatgatccca tttttgtacg ctctggtgtc
    74821 attaacctcg gcccggctga ctccgcattt ctcgccaacg tcgcccacag cgccgaacag
    74881 tggcaactca acgttgaaaa gctcgatgcg caagggatta tggcccgctg gccagaaata
    74941 cgcgtcccgg acaactacat cggcttattt gagactgatt ccggtttttt gcgcagcgaa
    75001 ctggcgatta aaacctggat ccaactggcg aaggaagcgg gctgtgcgca actgttcaac
    75061 tgcccggtca ccgcaattcg tcatgacgat gatggcgtaa ctattgaaac ggttgacggt
    75121 gagtatcagg cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga cctgctcccg
    75181 gagcagcctg tccagcccgt acgtaaagta tttgcctggt atcaggccga tggccgctat
    75241 agcgtgaaga ataaattccc ggcgtttacc ggtgaactgc ccaatggcga tcaatattat
    75301 ggttttccgg cagaaaacga cgcgttgaag attggcaaac ataacggagg ccaggttatc
    75361 cattcagcgg atgaacgtgt tccgtttgcg gaagtggtca gcgatggttc ggaagccttc
    75421 ccgttcttgc gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc
    75481 tatgataatt cgcctgacga agattttatt atcgataccc tacccggcca cgataataca
    75541 ctgctcatta ccggcctgag tgggcacggt tttaaatttg cgtcagtttt aggggaaata
    75601 gctgccgatt ttgcgcaaga caaaaaaagc gattttgatt tgacgccatt caggctttcc
    75661 cgcttccaat aatcatcatt acggcctcca gaatgaggcc gtttttacag gagtatttaa
    75721 tgcgtcggct gttgcactat ctca
//
LOCUS       DAZPBM010000002        67922 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPBM010000002 DAZPBM010000000
VERSION     DAZPBM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67922
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.002"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..424
                     /locus_tag="ACHUZQ_000076"
                     /pseudo
     CDS             1..424
                     /locus_tag="ACHUZQ_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
     gene            811..1443
                     /locus_tag="ACHUZQ_000077"
                     /pseudo
     CDS             811..1443
                     /locus_tag="ACHUZQ_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1546..2048
                     /locus_tag="ACHUZQ_000078"
                     /pseudo
     CDS             1546..2048
                     /locus_tag="ACHUZQ_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(2115..3572)
                     /gene="nuoN"
                     /locus_tag="ACHUZQ_000079"
     CDS             complement(2115..3572)
                     /gene="nuoN"
                     /locus_tag="ACHUZQ_000079"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1023894.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3579..5108)
                     /gene="nuoM"
                     /locus_tag="ACHUZQ_000080"
     CDS             complement(3579..5108)
                     /gene="nuoM"
                     /locus_tag="ACHUZQ_000080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1023895.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5272..7113)
                     /gene="nuoL"
                     /locus_tag="ACHUZQ_000081"
     CDS             complement(5272..7113)
                     /gene="nuoL"
                     /locus_tag="ACHUZQ_000081"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1023896.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7110..7412)
                     /gene="nuoK"
                     /locus_tag="ACHUZQ_000082"
     CDS             complement(7110..7412)
                     /gene="nuoK"
                     /locus_tag="ACHUZQ_000082"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1023897.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7409..7963)
                     /gene="nuoJ"
                     /locus_tag="ACHUZQ_000083"
     CDS             complement(7409..7963)
                     /gene="nuoJ"
                     /locus_tag="ACHUZQ_000083"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1023898.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7975..8517)
                     /gene="nuoI"
                     /locus_tag="ACHUZQ_000084"
     CDS             complement(7975..8517)
                     /gene="nuoI"
                     /locus_tag="ACHUZQ_000084"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1023899.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8532..9509)
                     /gene="nuoH"
                     /locus_tag="ACHUZQ_000085"
     CDS             complement(8532..9509)
                     /gene="nuoH"
                     /locus_tag="ACHUZQ_000085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1023900.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9506..12232)
                     /gene="nuoG"
                     /locus_tag="ACHUZQ_000086"
     CDS             complement(9506..12232)
                     /gene="nuoG"
                     /locus_tag="ACHUZQ_000086"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1023901.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12285..13622)
                     /gene="nuoF"
                     /locus_tag="ACHUZQ_000087"
     CDS             complement(12285..13622)
                     /gene="nuoF"
                     /locus_tag="ACHUZQ_000087"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1023902.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13619..14119)
                     /gene="nuoE"
                     /locus_tag="ACHUZQ_000088"
     CDS             complement(13619..14119)
                     /gene="nuoE"
                     /locus_tag="ACHUZQ_000088"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1023903.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14122..15924)
                     /gene="nuoC"
                     /locus_tag="ACHUZQ_000089"
     CDS             complement(14122..15924)
                     /gene="nuoC"
                     /locus_tag="ACHUZQ_000089"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1023904.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16018..16680)
                     /gene="nuoB"
                     /locus_tag="ACHUZQ_000090"
     CDS             complement(16018..16680)
                     /gene="nuoB"
                     /locus_tag="ACHUZQ_000090"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1023905.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16696..17139)
                     /gene="nuoA"
                     /locus_tag="ACHUZQ_000091"
     CDS             complement(16696..17139)
                     /gene="nuoA"
                     /locus_tag="ACHUZQ_000091"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1023906.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17768..18706)
                     /gene="lrhA"
                     /locus_tag="ACHUZQ_000092"
     CDS             complement(17768..18706)
                     /gene="lrhA"
                     /locus_tag="ACHUZQ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1023907.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19626..20843
                     /gene="alaA"
                     /locus_tag="ACHUZQ_000093"
     CDS             19626..20843
                     /gene="alaA"
                     /locus_tag="ACHUZQ_000093"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1023908.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20927..21526
                     /gene="yfbR"
                     /locus_tag="ACHUZQ_000094"
     CDS             20927..21526
                     /gene="yfbR"
                     /locus_tag="ACHUZQ_000094"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1023909.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21585..23417)
                     /locus_tag="ACHUZQ_000095"
     CDS             complement(21585..23417)
                     /locus_tag="ACHUZQ_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1023910.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23504..24152)
                     /gene="hxpA"
                     /locus_tag="ACHUZQ_000096"
                     /pseudo
     CDS             complement(23504..24152)
                     /gene="hxpA"
                     /locus_tag="ACHUZQ_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(24163..24657)
                     /locus_tag="ACHUZQ_000097"
     CDS             complement(24163..24657)
                     /locus_tag="ACHUZQ_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1023911.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24740..25195)
                     /gene="yfbV"
                     /locus_tag="ACHUZQ_000098"
     CDS             complement(24740..25195)
                     /gene="yfbV"
                     /locus_tag="ACHUZQ_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1023912.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25533..26735
                     /gene="ackA"
                     /locus_tag="ACHUZQ_000099"
     CDS             25533..26735
                     /gene="ackA"
                     /locus_tag="ACHUZQ_000099"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1023913.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26810..28954
                     /gene="pta"
                     /locus_tag="ACHUZQ_000100"
     CDS             26810..28954
                     /gene="pta"
                     /locus_tag="ACHUZQ_000100"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1023914.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29144..30664
                     /gene="yfcC"
                     /locus_tag="ACHUZQ_000101"
     CDS             29144..30664
                     /gene="yfcC"
                     /locus_tag="ACHUZQ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1023915.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30697..31239)
                     /gene="yfcD"
                     /locus_tag="ACHUZQ_000102"
     CDS             complement(30697..31239)
                     /gene="yfcD"
                     /locus_tag="ACHUZQ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1023916.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31297..31848)
                     /gene="yfcE"
                     /locus_tag="ACHUZQ_000103"
     CDS             complement(31297..31848)
                     /gene="yfcE"
                     /locus_tag="ACHUZQ_000103"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1023917.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31904..32548)
                     /gene="yfcF"
                     /locus_tag="ACHUZQ_000104"
     CDS             complement(31904..32548)
                     /gene="yfcF"
                     /locus_tag="ACHUZQ_000104"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1023918.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32684..33331
                     /gene="yfcG"
                     /locus_tag="ACHUZQ_000105"
     CDS             32684..33331
                     /gene="yfcG"
                     /locus_tag="ACHUZQ_000105"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1023919.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33388..33750
                     /gene="folX"
                     /locus_tag="ACHUZQ_000106"
     CDS             33388..33750
                     /gene="folX"
                     /locus_tag="ACHUZQ_000106"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1023920.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33771..34664
                     /locus_tag="ACHUZQ_000107"
     CDS             33771..34664
                     /locus_tag="ACHUZQ_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1023921.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34712..35602)
                     /gene="rpnB"
                     /locus_tag="ACHUZQ_000108"
     CDS             complement(34712..35602)
                     /gene="rpnB"
                     /locus_tag="ACHUZQ_000108"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1023922.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35799..36572)
                     /gene="hisP"
                     /locus_tag="ACHUZQ_000109"
     CDS             complement(35799..36572)
                     /gene="hisP"
                     /locus_tag="ACHUZQ_000109"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1023923.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36580..37296)
                     /locus_tag="ACHUZQ_000110"
     CDS             complement(36580..37296)
                     /locus_tag="ACHUZQ_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1023924.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37293..37979)
                     /gene="hisQ"
                     /locus_tag="ACHUZQ_000111"
     CDS             complement(37293..37979)
                     /gene="hisQ"
                     /locus_tag="ACHUZQ_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1023925.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38069..38851)
                     /gene="hisJ"
                     /locus_tag="ACHUZQ_000112"
     CDS             complement(38069..38851)
                     /gene="hisJ"
                     /locus_tag="ACHUZQ_000112"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1023926.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39072..39854)
                     /gene="argT"
                     /locus_tag="ACHUZQ_000113"
     CDS             complement(39072..39854)
                     /gene="argT"
                     /locus_tag="ACHUZQ_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1023927.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40120..40689)
                     /gene="ubiX"
                     /locus_tag="ACHUZQ_000114"
     CDS             complement(40120..40689)
                     /gene="ubiX"
                     /locus_tag="ACHUZQ_000114"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1023928.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40784..42301)
                     /gene="purF"
                     /locus_tag="ACHUZQ_000115"
     CDS             complement(40784..42301)
                     /gene="purF"
                     /locus_tag="ACHUZQ_000115"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1023929.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42338..42826)
                     /gene="cvpA"
                     /locus_tag="ACHUZQ_000116"
     CDS             complement(42338..42826)
                     /gene="cvpA"
                     /locus_tag="ACHUZQ_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1023930.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43085..43747)
                     /gene="dedD"
                     /locus_tag="ACHUZQ_000117"
     CDS             complement(43085..43747)
                     /gene="dedD"
                     /locus_tag="ACHUZQ_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1023931.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43737..45005)
                     /gene="folC"
                     /locus_tag="ACHUZQ_000118"
     CDS             complement(43737..45005)
                     /gene="folC"
                     /locus_tag="ACHUZQ_000118"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1023932.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45075..45989)
                     /gene="accD"
                     /locus_tag="ACHUZQ_000119"
     CDS             complement(45075..45989)
                     /gene="accD"
                     /locus_tag="ACHUZQ_000119"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1023933.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46145..46804)
                     /locus_tag="ACHUZQ_000120"
     CDS             complement(46145..46804)
                     /locus_tag="ACHUZQ_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1023934.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46887..47699)
                     /gene="truA"
                     /locus_tag="ACHUZQ_000121"
     CDS             complement(46887..47699)
                     /gene="truA"
                     /locus_tag="ACHUZQ_000121"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1023935.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47699..48712)
                     /locus_tag="ACHUZQ_000122"
     CDS             complement(47699..48712)
                     /locus_tag="ACHUZQ_000122"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1023936.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48778..49914)
                     /gene="pdxB"
                     /locus_tag="ACHUZQ_000123"
     CDS             complement(48778..49914)
                     /gene="pdxB"
                     /locus_tag="ACHUZQ_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1023937.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50013..51008
                     /gene="flk"
                     /locus_tag="ACHUZQ_000124"
     CDS             50013..51008
                     /gene="flk"
                     /locus_tag="ACHUZQ_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1023938.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(51005..52183)
                     /locus_tag="ACHUZQ_000125"
     CDS             complement(51005..52183)
                     /locus_tag="ACHUZQ_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1023939.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52458..53678)
                     /gene="fabB"
                     /locus_tag="ACHUZQ_000126"
     CDS             complement(52458..53678)
                     /gene="fabB"
                     /locus_tag="ACHUZQ_000126"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1023940.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53837..55843
                     /gene="mnmC"
                     /locus_tag="ACHUZQ_000127"
     CDS             53837..55843
                     /gene="mnmC"
                     /locus_tag="ACHUZQ_000127"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1023941.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55964..56242)
                     /locus_tag="ACHUZQ_000128"
     CDS             complement(55964..56242)
                     /locus_tag="ACHUZQ_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1023942.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(56276..56824)
                     /gene="epmC"
                     /locus_tag="ACHUZQ_000129"
     CDS             complement(56276..56824)
                     /gene="epmC"
                     /locus_tag="ACHUZQ_000129"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1023943.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56824..57633)
                     /locus_tag="ACHUZQ_000130"
     CDS             complement(56824..57633)
                     /locus_tag="ACHUZQ_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR1023944.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57633..58457)
                     /gene="mepA"
                     /locus_tag="ACHUZQ_000131"
     CDS             complement(57633..58457)
                     /gene="mepA"
                     /locus_tag="ACHUZQ_000131"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1023945.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58461..59546)
                     /gene="aroC"
                     /locus_tag="ACHUZQ_000132"
     CDS             complement(58461..59546)
                     /gene="aroC"
                     /locus_tag="ACHUZQ_000132"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1023946.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59581..60513)
                     /gene="prmB"
                     /locus_tag="ACHUZQ_000133"
     CDS             complement(59581..60513)
                     /gene="prmB"
                     /locus_tag="ACHUZQ_000133"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1023947.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            60679..61230
                     /gene="smrB"
                     /locus_tag="ACHUZQ_000134"
     CDS             60679..61230
                     /gene="smrB"
                     /locus_tag="ACHUZQ_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1023948.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61352..62224)
                     /locus_tag="ACHUZQ_000135"
     CDS             complement(61352..62224)
                     /locus_tag="ACHUZQ_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1023949.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(62211..62735)
                     /locus_tag="ACHUZQ_000136"
     CDS             complement(62211..62735)
                     /locus_tag="ACHUZQ_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1023950.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62732..63202)
                     /locus_tag="ACHUZQ_000137"
     CDS             complement(62732..63202)
                     /locus_tag="ACHUZQ_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1023951.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(63199..63747)
                     /locus_tag="ACHUZQ_000138"
     CDS             complement(63199..63747)
                     /locus_tag="ACHUZQ_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1023952.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63722..64473)
                     /locus_tag="ACHUZQ_000139"
                     /pseudo
     CDS             complement(63722..64473)
                     /locus_tag="ACHUZQ_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64493..67135)
                     /gene="yfcU"
                     /locus_tag="ACHUZQ_000140"
                     /pseudo
     CDS             complement(64493..67135)
                     /gene="yfcU"
                     /locus_tag="ACHUZQ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(67217..67780)
                     /locus_tag="ACHUZQ_000141"
     CDS             complement(67217..67780)
                     /locus_tag="ACHUZQ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1023953.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16998 a        18334 c        16853 g        15737 t
ORIGIN      
        1 tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggata
       61 aatgtcgaat aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc
      121 cgttcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga
      181 agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta
      241 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt tttaacatcg
      301 actggtaaac aggaattgtt atttagataa atatggcgat gcaaatatca atgattatta
      361 tagagactta gaaataaaac actcacaacg caaaaataga gcatcaggaa aaagagtaag
      421 ttaacatcat tgttaattaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt
      481 ttgctaaaca gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcttcggg
      541 tatttccagc gtatctcggc acgcgattgc tggtctgttg atgtagtgga ttgttgttgc
      601 tgacttttct taccctcaga gttctgcccc ctgtcaactc aatctgccaa taaataacat
      661 aattatattt ttccttatat gaattccttt ttaaaatagc gaagccactg taaaagatac
      721 tttttgtata gatctaacaa ccgtaataaa acaaacactc ataactcatt ataaataatg
      781 ataaaataac gtcttttatt gataatttat atatggaaaa aaattccttt tgttatattg
      841 tttctgattt cactccctct gattattttt accaaccctg ggtcactgca ttatcgccaa
      901 cgccccgaca cgtcagtcct gagcaattag aaaaaacagt tcgttatctt acacaaactg
      961 tgcatccacg taatgccgac aatattgata atctgaatag gtccgtagag tatataaaag
     1021 agttctttgt cagtagcggt gccagagtta ccgcgcagag cgtccccgat tggctattac
     1081 gactctgcgc ctggtagcca ggattaccct tacccgcaat gtcctggctt tatcccgatc
     1141 ggggggactt tattgcctgg tcggcagaat acaggatatc aacgccgttc gtcaggtaaa
     1201 aacggcattg ttatcatccc gggatttatc tgtttattct atgaatgccc catggtttat
     1261 tcccggtatt gatttctctg accacctgaa ttattggcaa cacgatattc ccgccgtaat
     1321 gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg atacagcaga
     1381 cagattgaat tatcagaaaa tggctcagat ggtgttataa ccttgttaca caacagtaaa
     1441 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1501 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggt
     1561 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1621 ttgatggata atatacatga cacgctttca ggattacgcc gactggatat tgataaacgc
     1681 tgggattttt tacactttgg gctgacaggc acccctgctt ttgatcccgc gaagaatgat
     1741 ccgctgagtc gtgccgtgct tggtgaacac agcctcgaag atggcataga tggcttcctg
     1801 ggattgacct ggaatcagga gttagccgcg acaatcgacc gactggaatc actggatcga
     1861 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1921 gtgacctttt cagaagagtt agaggggcaa ctctttgctt cgatcatgct gatatggaaa
     1981 agctgattag cgcgtatcga cgtatgctgc gacaagggaa tcatgcgctg acggttattg
     2041 ttgggtgatg gtgtcagggc accaaaaaaa accgccgaac atgtcggcgg ctttctgact
     2101 tacaaactga tagcttacat cagcggcatt gctaaccgca caatgctaat cagcggttgt
     2161 ggccatacac ccagcaccag taccaacagt gcagagatca gcaccacgat accgcctgcg
     2221 ctgtactgcc agtttgacgg tgcatcgcga cccggttgtt ccggggcgtg caggtacagg
     2281 ctcaccgcca cgcgcaggta gtagtaaagg ccgcttgccg aaccgacaac cacggcgccc
     2341 accagccacc acaagtgtgc ctggacaccg accgccagca cgtagaactt accaataaag
     2401 cccagcgtca tcggaatacc ggccagagac agcatcatca ccgtcatcac cgccgcgagg
     2461 atcgggcgat gccagaacag tccgcggtaa gagtacaggg aatcagcatc cgggccacga
     2521 tacgggctgg acatcaggct gaccacgccg aacgcgccga ggctgctaaa cagataaccg
     2581 gccaggtaaa cccctaccgc ttccatcgac atctcgccag tttgcagcgc aatcagcgcc
     2641 accagcagat agccgaggtg agagatagat gagtaaccga gcagacgttt gatattggtc
     2701 tggctcagcg ccatcaggtt accgaagatg atggaggcaa aggcgataat cgccagcacc
     2761 acgcgaatcg cttcgctgtc acccatcggt gcgtagagga acagacgcat caccacaccg
     2821 aagatagcga ttttgctcgc cgtcgccagg aaagtggaaa ccggcgcagg cgcgccctgg
     2881 tatacgtctg gcgtccacag gtggaacggc accagagaga gtttgaagcc gaggccaaca
     2941 atcatcaggc cgaaacctgc cagcaacagc ggctcgttga gcataccgtc gccaaggttt
     3001 ttacccaacg cgacaaacga caggtcgcca gactgcgcat acaccagcgc cataccaaac
     3061 agcaggaaag aagacgctgc ggcagaaagg atggtgtatt tgatactggc ttccagtgaa
     3121 cgtttctggc ggaaagcgta accgaccagg ccaaacagcg gcaaagagat cagttcgata
     3181 ccgaggaaca gagacgccag atggttggca ttcgccagca ggatcccgcc cagcgcggca
     3241 attaacacca acaggtagaa ctcgtccttg ttgtcgttat agccttcaag ccacgggtag
     3301 gcgaaagtac aggtggcgag gctcgccaac aataccagcc cggtgtaaag catggcgaaa
     3361 ccatcaacgc gcatcagcgg cgtaacgtcc atagcgcctg cctggccgac aaaccagagc
     3421 gaaaccagcg ccgcgttaag cccaataacc gagagcgtag cgttgaggaa atgattgcgt
     3481 cgccacgcaa tggagagcat cacaaccacc accgtcaagc cgacgatcag caacggtagc
     3541 agtgcgatca ggttttgtgg agttattgtc atggcgattt acggccttgt agtagtaacg
     3601 gaattaacaa accactgctg gatattgcca atcgcggagt gcgaggtatc cagaatcggc
     3661 tgcggataga agccctgcag taccagcagc accaccagca acaggatcat aaacagctca
     3721 cgcagcgaca tccctggcag ttcctggctg gcaatctcgc ttttcgcttt accgaagtaa
     3781 gcgcgatgca acatcgccag cgaataaaca gatgcaaaga ccagcccaaa ggtagagata
     3841 acggtaatca ccgggacaac ctggaagctg ccgaacagaa tcataaattc gccgacgaag
     3901 ttaccggtgc caggcatccc aagcgttgcc accgcaaaga acagcgacag tgctggcagc
     3961 catttcatct tgctccacag accgcccatc atgcgcatgt cgcgggtatg gatacgttca
     4021 taaagctgac cacaaagaat aaacagaccc gccgccgaca agccgtgcgc aatcatctgg
     4081 attaccgcgc cctggtaggc caactggctg ccggtgtaga tagcaatcag cacgaagccc
     4141 atgtgggaaa ccgaggtgta ggcgatcaga cgtttgatat cggtctgggc gaaggccatc
     4201 cacgcaccgt agaagatgcc gataacaccc agccacatag caattggcgc gaactctgcc
     4261 gacgcgttcg ggaacagcgg cagggagaaa cgcagcaaac cgtaagcggc agttttcagc
     4321 aagatccccg cgaggtcaac ggaaccggcg gtcggagcct gggagtgcgc atccggcagc
     4381 cagccatgca gcggaaccac cggcattttg actgcgaagg cgatgaagaa gcccagcatt
     4441 aacaggtatt ccgcaccact ggacattggc gtattcagca gctcttcata gttgaaggtc
     4501 cagacgccgg tcgcattgta gtgaacaaaa accagcgcca ggatggcaat caacatcacc
     4561 agaccactcg cctgggtgta aatgaagaac ttggttgccg ccgtgatacg cgttttaccg
     4621 tcagaggctt tatgccccca cagtgcgatc aggaagtaca tcggcaccag catcatttcc
     4681 cagaagaaga agaacaggaa catgtcgatg gcaaggaaca cgccgataac gccgcccagg
     4741 atccacatca ggttgaggtg gaagaagccc tgatattttt cgatctcttt ccacgaacat
     4801 agtaccgcca gcacaccgag cagaccggtc agcacgacca tcagcagcga cagcccgtca
     4861 atggcgagat gaatagagat acccaaacgc gggatccacg gcatgtcgaa ttcagactgc
     4921 cactgcggaa ttccggcgga ttgcgtcagt gaataaccgc cctgcaacca cagttgcagc
     4981 gacagcgcca gcgtcaatcc catggtgatc agcgcgatcc agcgtggcac cttgacgcca
     5041 aagcgttcgg tctgccagca caggaagccg ccaataaagg gaattaatat tagccagggt
     5101 agtaacatgg cgatctttat tccttgtaaa agtcccgtca ggaccggatt ttcaacgaat
     5161 tctcacgaca gaaaattctc ttcggactgg gggtagtgtc ggatgcaccg cgaaggcggc
     5221 ttatccgacc tacgttctta tatgcccagg ggcaatccca caatccttaa ctcaacgcag
     5281 taccatcaac agtgccagca ccacgaccgc accgatgctc atggatgcca cataccagcg
     5341 cagatagccg ttctcactta acagcagacc tttacctgca aagcgggaaa ggacagccgg
     5401 aatgttcatc attgagttca gcggatcgcg tttcagcaac caggcaatac ccaggaacgg
     5461 cctgacgaac actttgtcgt acagccagtc aaagccccag gcgttgtacc accaggtact
     5521 cagcaggcgg cccggcgcac tgttggcgat ggaggtcacc agagtacgtt tacccagcca
     5581 cagccaagct gccagcagaa tgccgacaat cgcgaccacg ccagaggtaa tttccagggt
     5641 caacatgctg ccgtgcgcca gttccgtcgt ttgcggaagc acgccctgca gcggcggtac
     5701 aatcagtgcg ccaacgaagg tggaaaggat cagcagcaca atcagcggca ggctgtgagt
     5761 tacccctttc acggcgtgag cgtgaatttg ttcttttccg tggaagacga tgaaaatcat
     5821 acggaaggtg tagagcgagg tcataaacgc accgaccaga cctgccacca tcagattgat
     5881 atgaccattc gccatcgcac ccgcgaggat ctcatcctta ctgaagaagc ccgcagtgac
     5941 cagcggcagt gccgacagtg ctgcgccgcc caccaggaag cagagataaa ccagcggaat
     6001 agatttacgc agaccgccca tcttgaagat gttctgttcg tgatggcagg ccagaatgac
     6061 ggaaccggat gccaggaaca gcagcgcttt aaagaacgcg tgagtcatca agtggaaaat
     6121 cgccgcatcc catgcctgca cgccaagcgc gaggaacatg tagccaatct ggctcatggt
     6181 agagtaagcg agaacacgtt tgatgtcggt ctgtaccagc gcggcaaaac cggccagcag
     6241 cagcgtaacc gccccgacaa tgcccaccag atgcagaatt tccggcgtca tcaggaacag
     6301 gccgtgggta cgggcgatca ggtagacacc cgcggttacc atggttgcgg cgtggatcag
     6361 cgcggagaca ggcgtcgggc ccgccatcgc gtcggcaagc catgtctgca acggcaactg
     6421 cgcagattta ccgaccgcac cgcccagcag catcagcgtc gcccacatca gcatgttatt
     6481 gccgtcagca aagtgcgctg gtgccagttc caccatttcg cggaagttca gggtgcccag
     6541 ttcgttgtaa agaatgaaca gtgcgaaagc gaggaaaacg tcacccacac gggtcacgac
     6601 gaacgctttc attgccgctg cgccattctt cggatcggtg taatagaaac cgatcagcag
     6661 ataggagcac aggcccacgc cttcccagcc gaggtacatc agcagcaggt tgtcggcaag
     6721 caccagaact accatgctgg cgatgaacag gttggtgtag gcgaagaagc gagagtagcc
     6781 ctcttcaccg cgcatgtacc aggaggcgta catgtgaata aggaaaccca caccagtgac
     6841 caccgagagc atggtcagcg acaggccgtc cagcaccagg ttaaaaccga tgttaaagtc
     6901 gcctaccgac atccacgtcc acagcggctg gctgtatgcc tgctcgccgt tagcgaagaa
     6961 atcaacgccg ataaaggcgg ttaccagcgc cgccaggccc acagagccaa cgccgacgat
     7021 cgccgaaacg ttttcagacc agcgcccacg ggagaacgcc agcaggacga agccaatcaa
     7081 tggcaaaata atggttaagg caagcatgtt catccgcgca tctcacttac tgaatcgatg
     7141 ttcaggttct ggcgacgacg gtgaagttgc agcagcagcg caaggccgat actcgcttct
     7201 gccgccgcga ggctgatggc gagaatgtac atcacctgac cgtcggtctg accccagtag
     7261 cttccggcga caacaaaggc cagcgcggag gcgttaatca tgatttccag accaatcagc
     7321 ataaacagca gattgcgacg gataaccaga ccggttaagc caagaatgaa taagattgcc
     7381 gcgaggatca gtccatgttg taaggggatc atgcgtgctc ctccgttttt cttttcgcgc
     7441 tgtcgtcttt acgattgctc agcacttcac ccgcacgctc ttcacgaccg acgtggaagg
     7501 ccacaaccag acctgcgagc agcagcatag aagccagttc caccgccagt acgtaaggcc
     7561 cgaacagcgt aatacccact gctttagcac tgattggcgt accgtcgata ccctgatcgt
     7621 taacaccgag gatggcgtaa acaatcacca ccagcatgat ggccgacaaa attgccggac
     7681 caatccacac ctgcggtttc agccactggc gttcctgttc gatttctgaa ccgcccaggt
     7741 tgagcatcat caccacgaac acgaacagca ccataatggc acccgcgtag acgataattt
     7801 ccagcgcacc ggcgaagtaa gcgcccagtg agaagaacac cccggaaatc gccagcagcg
     7861 aaataatcag gtacagcagt gcgtgtaccg gattggtatg ggtgatcact cgcaaggtcg
     7921 caagtatggc tatcaggcca cagatataaa aagcgaactc cattgcccct ctccttacgg
     7981 taacaggctc ttgacgtcga taggcttggc ttcgttctct gcttcgccct tatctttgcc
     8041 gtcgattgcc atacctgcca tccggtagaa gttatattcc gggtatttgc ccggaccgga
     8101 gatcagcaga tcctcttttt cgtaaaccag atcctggcgc ttgtattccc ccatttcgaa
     8161 atccggggtt aactgaatcg ccgtggtcgg acaggcttct tcgcacagac cacagaaaat
     8221 gcagcgtgag aagttgatgc ggaaaaattc cggataccag cgaccgtctt tggtttctgc
     8281 tttttgcagc gagatacagc cgaccgggca ggctaccgcg cagaggttac aggctacgca
     8341 acgctcttcg ccgtccgggt cgcgggtcag aacgatacga ccacgataac ggggcggcag
     8401 atagatcggc tcttccgggt acattcgcgt ttcgcgtttg gcgaacgcgt gcaggccgat
     8461 catccagata ctacgaacct gggtgccgaa acctactaac aattctttta aggtcatggt
     8521 cttattgccc cttattgcgc ctgccagaga atgacagccg ccgttaccag caagttgatc
     8581 agcgtcagcg gcaggcagat tttccagccg aaggacatta cctggtcata acgcggacgc
     8641 ggtaacgacg cacgaatcaa aatgaacatc atcataaaga acgcggtttt cagcgcgaac
     8701 cagatgaatg gcggtaacaa cgggccttgc cagccaccga agaacagcgt caccatcaat
     8761 gcagagatgg tcacaatccc gatgtactca cccacgaaga acagaccgaa cttcataccg
     8821 gaatattcaa tgtggtaacc atccgccagt tcctgctcgg cttccggctg gtcaaacggg
     8881 tgacggtgac ataccgccac gcccgcgatg gcaaaggtaa taaaaccaaa gaattgcggg
     8941 ataacgttcc acacatgcgc ctggctgttg acgatgtcgg tcatgttgaa tgaaccggcc
     9001 tgcgccacca cgcccatcag ggaaagcccg aggaacactt cgtagctcag ggtctgcgca
     9061 gaagcacgca tcgcacccag caacgagtat ttgttgttac ttgaccagcc cgcaaacagc
     9121 accgcgtaaa ccgccagacc tgccatcatc aggaagaaca aaatcccgat gttcaggtcg
     9181 gcaaccaccc aacccggact gactggcaca atcgcaaagg ccagcagcag cgaggtaaag
     9241 gcaatcatcg gtgccagggt aaagatgacg cgatccgaga atttcgggat ccagtcttct
     9301 ttaaagaaca ttttgatcat gtccgcaacc agctggagcg aaccgcccca gccaacacgg
     9361 ttaggtccgt aacggttctg gaacagaccc agcaggcgac gttcgccaaa gctcatgaat
     9421 gccccgcagg tgacaaccac cagcaggatc accaccgctt tgaggatggt cagcaggatc
     9481 tcaatcagtt ccggtgatat ccaactcatt gttgtgcctc cttgagatcc tcaagatgcg
     9541 cgccagccag caccggagca ataccggaca tacccatcgg caagcccacc tgccctgccg
     9601 tcagcccttc agcgatttca accggcagcg tgaccgtatt gccatcgtaa ctaaaggaga
     9661 cgtgtgtacc tgcgttcaca cctaacttcg cggcatccgc tgggttgagt ttgatgtacg
     9721 gctgcggcat acggctctgg aagaccggag cacgctgtga caattcatcg ctgccaaaca
     9781 ggtggtaata cggcgcgata cgccatttcc cgtcctgcgg ctggaagcgt gccggtacgc
     9841 tggtgaagta atccagacca ttttcgctgg tttcaaacag acgcacgccc ggatcgccaa
     9901 agcgcagttt gccgcccact tcgtcctgga atttgttcca cgcctgcggg gagttccagc
     9961 ccggcgccca ggcaaacggc acttgcgaac ggtgcgcagt cggctggttg ttaccttcca
    10021 tcgagaaggt gaacatggtg tcaatatcct gcggctgacg cggctcatga acgctgatat
    10081 tggcgcgcat ggcggtacga ccgctgtaac ggtgcggttc acgggccagt ttctgcccac
    10141 gaatacggaa tgtcgcatcc ggcgcagcat ctttgatacc tgccagttcc gggattttcg
    10201 ccacaacagc gtcaatcaca tggtcgagct gcgtccagtc cacttcacgg ctcagcaggg
    10261 tgctgtgcag cgagtgtaac cagcgccagc tttccagcat gacagttttg ctgtcgtaat
    10321 aagcaggatc gtaaacctgg aagaaacgtt gggcgcggcc ttcgttgttg atcaccgtac
    10381 cgtcgctttc agcaaaactg gcagcagaaa gtaccagatg ggcgttttcc ataatcgctg
    10441 tgcgttgatg atcaaccacc atcaccagcg gcgctttagc cagcgcagca ttcacgcggg
    10501 tagcggaagc gtgacgatgc agatcgtttt ccagcaccac caccgcgtcg gcgcgtccgg
    10561 tttccagttc ggtcaacgct tcttcaagcg aaccgccacc cataatgccc agccccatgc
    10621 tgttgacgga acgggcaatc atggtgatac caacgtcagc gccgcgacct ttcagggctt
    10681 tcgcgacgtt agccgcagcc tgaatcacct ctatgctacc ggcgttcgtc ccggagataa
    10741 tcaacggttt cttcgcacct gccagtgcct gcacgatgac gtcgattttg ctttgcagct
    10801 caggttcgat accgtcaacc gctggcgcag agttatccag cgcatgggcg atggcaaaac
    10861 ctaaacgcgc ctgatcttca accggtgcgc ggtaagtcca cgccgcgata tcatccagac
    10921 gggtgtcatc aacgttggta acaaacagcg gatgcttcgc acgttgaccg atgttgagga
    10981 ttgccgcaat ctgccagtca gccactttct gtgctgccgc catttcgcgc gctttacctt
    11041 tcacagcctg acgcactgcc agcgcgacgc gcgcgccggt ctgggtaacg tcttcgccca
    11101 gcaccagtac cgcatcgtaa gattcgattt cgcgcagagc cggagtataa atgccgcctt
    11161 cacgcagcac tttcagcgcc agttgcagac gttcctgctc accgtgagcg ataccggtgt
    11221 agaagttttc ttcgcccacc agttcacgca gcgcaaagtt gctttccacg ctggcacgcg
    11281 gagaaccaat accgatcact ttcttcgact gacgcagaat atctgccgcg ccctgcattg
    11341 cctgttcggc gttgagggta atgaaatcat cgccacgacg ctgtactggc tgacgcggac
    11401 gatccttcag gttgacgtaa ccgtaaccga aacgaccacg gtcgcagagg aagtagtggt
    11461 ttaccgtacc gttgtaacgg ttttcgatac gacgcagttc gccgtaacgt tcaccggggc
    11521 tgatgttaca gccgatggaa cattgctggc agatgctcgg cgcaaactgc atatcccatt
    11581 tacggttgta acgctcggag tgcgttttgt cggtaaatac gccggtcggg caaatttcga
    11641 ccaggttacc ggaaaattcg ctttccagcg tgccgtcttc cgggcgaccg aagtagacgt
    11701 tgtcgtgcgc accgtaaacg cccagatctg taccgtcagc gtaatctttg tagtaacgca
    11761 cacagcggta gcaggcgatg cagcggttca tttcgtgaga gatgaatggc cccaaatcct
    11821 gattacggtg ggtacgtttg gtgaaacggt agcgacggaa gctgtgtccg gtcatcacag
    11881 tcatatcctg aagatggcag ttaccgcctt cttcacatac cggacagtcg tgcgggtggt
    11941 tggtcattaa ccactcgacc acactttcac ggaactgttt cgcttcttcg tcgtcaatgg
    12001 aaataaaggt gccatcggaa gccggtgtca tacaggacat caccaggcga ccacgcgtgt
    12061 cttccgcgtt ttggtattgc ttcaccgcac actggcggca agcaccgacg cttcccagcg
    12121 ccggatgcca gcaaaagtaa ggaatatcaa ggcccagaga cagacaagct tccagcaggt
    12181 tgtccgctcc gttgacctcg tattctttgc cgtctacatg aattgtagcc attagcatgc
    12241 ttccagtttt ctcggtcagg gaccgagcgt taatcgaaat tcggttacca gcgctctttc
    12301 agcaggttcg gctgaatccc attaatcaaa tgggtattgc tgaacggctg tttgattccc
    12361 gcctcaaatt cttcgcggaa atatttgatg gcgctctgta acggctccac tgcaccaggt
    12421 gcgtgggcac agaaagtttt acccgggcct aagaatcgac acagttgctc aagtgtttcg
    12481 atatcgcccg gctggccttc gccacgctcc agcgcacgca gaattttcac gctccacggc
    12541 agaccgtcgc ggcacggcgt acaccagccg caggactcac gggcgaaaaa ctcttccagg
    12601 ttacgcacca gcgacaccat gttgatctca tggtcaaccg ccatcgccag cgccgtaccc
    12661 agacggctgc ccgctttacc gatactttcg aattccatcg gcagatcaag gtgcgcttcg
    12721 gtcaggaagt cagtccccgc gccgcctggc tgccaggctt taaatttcag accatcacgc
    12781 ataccaccgg cgtaatcttc gaggatctcg cgtgcggtgg tgccgaacgg cagttcccac
    12841 agtcccggat ttttcacccg accggagaag cccatcagct tggtgccagc atctttactt
    12901 ttcgagatgt tctgatacca ctccacgccg ttagcgagga tcgccggaac gttacacagg
    12961 gtttcgacgt tgttgacaca ggtcggtttg ccccatacgc cggaggttgc cgggaagggg
    13021 ggtttcgagc gtgggttagc acgacgccct tccagggagt tgattaacgc tgtttcttcc
    13081 ccgcagatgt agcgccctgc cccggtatgg acgaacagtt cgaagtcgaa acctgttccc
    13141 ataatgtttt tgccaagcaa gcccgcttca gtggcttcgg caatggcgcg gcgcagattt
    13201 actgccgctt cgatatattc gccacgcagg aagatgtagc cacggtaagc tttcagcgca
    13261 aacgcggaga tgagcatacc ttccaccagc aggtgcggca gttgctccat caacaggcgg
    13321 tctttatagg tgcccggctc catttcatcg gcgttacaca gcaggtaacg gatgttcatg
    13381 gattcgtctt tcggcatcag gctccacttc aggccagtcg aaaagcccgc tccgccgcgc
    13441 cctttcagac cagcgtcttt tacctgatta acgatttcgt ccggagacag cccggtcagc
    13501 gccttacgcg ccccttcgta accgtttttg ctgcggtatt cgtccagcca cactggctgt
    13561 ttgtcatcgc gcagacgcca ggtcagcgga tgcgtttcgg gagtacggat aatgtttttc
    13621 atttataccg ctccagcagt tcagggatcg cttccggggt cagatgcgcg tgagtgtcct
    13681 catcgatcat catgtttggc cctttatcac agttccccag gcagcaagtt ggcagcagcg
    13741 taaagcggcc atcaaatgtc gtttgccctg gtttgatgtt cagctttttc tcgagcgccg
    13801 cctgaatacc ctgatacccg ttgatatgac agaccacgct gtcgcaataa cggatcacat
    13861 ggcgaccaac cggctggcgg aagatctgac tgtagaacgt tgccacacct tcgacgtcgc
    13921 ttgccggaat acccagcaca tcggcgatcg cgtggatcgc accatccggc acccagccac
    13981 gctgcttctg aacgattttc agcgcttcaa tggacgccgc acgcgggtct tcgtagtggt
    14041 gcatctcgtg ctcaatcgct tcacgctctg ccgcactcag ctcaaaagcc tcggtttgtg
    14101 gttgttgatt ctcgtgcata attagcggtc cacatctgac ataacaaaat cgatactgcc
    14161 cagataaaca atcaggtcag acaccaggct gccgcggatc gccgccggaa tttgctgcaa
    14221 atgcgcataa ctcggcgtgc ggatacgagt gcgataactc atggtgctac cgtcgctggt
    14281 caggtagtaa ctgttgatcc ccttggtcgc ctcaatcatc tggaaagatt cattggcagg
    14341 catcaccggc ccccacgaca cttgcaggaa gtgggtgatc agggtttcga tatgttgcag
    14401 cgtgcgctct ttcggcggcg gcgtggtcag cgggtgatcc gctttgaacg ggccttccgg
    14461 catgttgttg aggcactgct caagaatgcg cagactctgg cgcagctctt ccactttcag
    14521 cattacgcgg gtgtagcagt cagaaacacc accaccaacc gggatttcaa agtcgaagtt
    14581 ttcatagcca gaataaggac gcgccttacg cacgtcgaag tcgatcccgg tagcacgcag
    14641 gcccgcgcca gtggtgcccc actccagcgc ctctttcgcg ccataggcgg caacgccctg
    14701 ggaacgacct ttcagaatgg tattttgcag cgccgctttc tcgtaagacg ccagacgttt
    14761 cggcatccag tcgaggaact cacgcagcag gcgatcccag ccgcgcggca ggtcgtgcgc
    14821 tacgccgcca atacggaacc acgccgggtg catacggaaa cccgtgatcg cttccaccag
    14881 atcgtaaatt ttctgacgat cggtaaaggc gaagaacacg ggcgtcattg cgccgacgtc
    14941 ctgaataaag gtcgagatgt acagcaggtg gctgttgata cggaacagtt cggagagcat
    15001 aacgcgaatg acgttaacgc gatccggcac ggtgatcccg gccagtttct ctaccgccag
    15061 cacgtaaggc atttcgttaa cgcagccgcc gaggtattca atacggtcgg tatacggaat
    15121 gtagctgtgc caggactggc gctcgcccat tttctccgca ccacggtggt ggtagccgat
    15181 gtctggtacg cagtcgacaa tctcttcgcc gtcgagttgt aaaacgatac ggaaagcccc
    15241 gtgcgccgac gggtggttcg gaccgaggtt gaggaacatg aagtcctcgt tttcggtgcc
    15301 gcgcttcatc ccccactctt ccggtttgaa ggtcagggct tccatctcca gatcctgttt
    15361 ggctttggtc agctcaaacg gcgagaattc ggtagcgcgc gccgggtaat ctttacgcag
    15421 cgggtgacct ttccaggttt gcggcatcat gatgcggcgc aggttcgggt gaccgtcgaa
    15481 agtaatgcca aacagatccc aggtttcacg ctcataccag ttagcgttcg ggaacagttt
    15541 ggtgaaggtc ggtacgtgca ggtcgttttc tgccagcgcc accttcagca tgatgtcgcg
    15601 gttacgatcg atagaaatca gatggtagaa aacggaaaaa tccgcggcag gtaacccttc
    15661 gcggtgtgtg cgcagacgtt cgtccatgcc gtgtaagtca aacagcatga cgtaaggttt
    15721 cggcagtttc tttaagaaat cgccaacttc cagtaattgt tcacgcttga tccacacaac
    15781 gggaaccccg gtgcgagtcg cctgaacagt aaaggcatcc ggcccaaaac ggttgcgcag
    15841 ttcgccaatc accggatcat caagatgatc acgggtctgc caggcgggtt cttgcgcggt
    15901 taagtcggtc atattgttca ccattgcaaa tggtccgtgg tgactgtcgg cgcagtaagc
    15961 ttcgcgaaat agatttagga ataagcgaag aaaaatccct ttgccgacag gcgcaaatta
    16021 aatctcgtca ggtgtacgca ggttggttac ggcaatgcgt tcaccgcgct tgcgttcgcg
    16081 ctctgattgc atattggcgc gataaacgcc ctgatcgcca accacccagg agagcggacg
    16141 acgttctttg ccgatagatt cctgcaacag catcagtgcc tgcatgtacg cttcaggacg
    16201 cggcgggcag cccgggatat acacatcaac cgggatgaat ttatcgacgc cctgcacaac
    16261 ggaataaata tcgtacatac caccagagtt ggcacaggca cccattgaga taacccattt
    16321 tggttccagc atctggtcat acagacgctg aataaccggt gccattttgg taaagcaggt
    16381 tcctgcaacc accatcaggt cagcctgacg cggcgaagca cgcaatactt ctgcgccaaa
    16441 acgcgccacg tcatgcaccg cggtaaacga agtcaccatc tcaacgtaac agcaggaaag
    16501 accgaagtta tacggccaaa ttgagttttt acgaccccag ttaaccatgt cattgagctt
    16561 gcccataaac acgtttttgt taacttcttg ctccagaggg tcggttacga tctcctgctt
    16621 ttgcaggggg taacggtcgt tctcaccgtt gggatctatg cgggtgagcg tataatccat
    16681 cttaatgcct cgcggttagc gttgacgatt agcgatactg ttcgtttccg ggttcatacg
    16741 ctcgcggcgt gaacgcgcgg gcgtccagtc cagcgcgcca atacgcacca gataaaccag
    16801 acctgccagt aacacaaaaa taaaaattgc agcttccaca aagcctaccc agccgctttc
    16861 gcggatagag gttgaccatg cgaacagata cagcgcttca acgtcgaaga taacgaagaa
    16921 catggccacc agataaaact tggcggacag gcgtaagcgg gcggagccga ccgagtcgat
    16981 accggattca aacggcacgt ttttcgacct cgcgcgtgcg cgaccgccta aaaaccaacc
    17041 gcctaccagc atcaggcaac acaggccaat ggcaacgata agaaagatag cgaatgccca
    17101 gtgatgagcg atgacttcag tggatgttga catactcatt gcttactcat caaaagtagc
    17161 gccagattca ctgctcttca cggcagatgg acgccacatc gattcatggg gaggaataaa
    17221 aaaaacctta caatcactgt agaaattctt ttatacagct aattgatgtg gtcttttact
    17281 cctttctata accttttgtc aactttaaca aaagtttctt cacattagtt tacataatat
    17341 caacaccatt agcatttaat gccctttcac gccagatcct tgacgactcc aggataatta
    17401 gatgttgttg aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag
    17461 gaatttgtgt cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt
    17521 gtcattccat aacaaaaacg cagcaacaaa tttacgtatt tttaacatca ttgtagcagg
    17581 tgattttttt caggcgatta tttgtgcgtt cgggacgtga atctctggtg attgaaaaat
    17641 gaacagtttt gtacgttctg cactatgcga ggaaggattt ttactaaaaa aaagccgctg
    17701 gggtttaaaa cacccccagc ggctcgtttt tacactattg tctcaggaat tatctatcgt
    17761 ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc atccccttcc ggagcagaca
    17821 ttgggctgta ttgccacgga ttatggtagc tctccatcgc ctgatagatc acctgggcca
    17881 gctcattatt actggacggg tcatagcaca gcaaatattc ggtatcaggt agcggcggta
    17941 agccatctac tccactcagc acacgcaggt ccgggctcat catttcaatc ggccttgccg
    18001 tcacgccaag accggctttc actgccgcac gaacggccgg aagcgtcgag gcgacataag
    18061 ccagtcgcca tggaatatct gctttattaa gcgtcgccag caccatatcg cgaaacggac
    18121 ttggatcatc cagcaataca agagggatcg gctcgccttt ttgcaatacg tattccgctg
    18181 cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc cttaaacgct gaaggacgat
    18241 gggtggttac catcaaatcc acttcctggg attccagcat ttctgccata taggcattac
    18301 gtttaacgcg gacatccagc gctaatttcg gataaaccga actcacgcga tttaacagga
    18361 aaggtaagat cgtatcggca gattcatctg aagcaccgat agttaacacg ccctgaagat
    18421 tactgaacat taatgatgag caggcctcat cattaaaacg cagtattttc ctggcgtaac
    18481 caagaagttg aatgccatgt tcagttaaca gtttgttgcg accgtgacga gcgaacagtt
    18541 ctttcccaac gagttgttcc agacgctgca tttgctgact tacggcggac tgagtacgac
    18601 acacagcggc agctgcggca gcaaaagtgt tcagatcggc aacagcaaca aatgttctca
    18661 gcagatcgag gtcgaggtta attatcggac gatttgcact tatcatatat tatcacttac
    18721 tggcggctca tactgagctg gttaatgctg tgcacacaca aacaagcaat tccatttgta
    18781 atgtgcctcc ctggcagttt catccggaac ccggacgaaa gtaaaaatgc atatgagttg
    18841 cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg aagtagatca cagaatatat
    18901 cttcagggat cgcatatcta ttaagttact cactcttttc tatttatgac atgcgcgtgt
    18961 ttgtataaat gtaaatgtga gtccttgttc cactctcgtg cagcatcgct ggtcatacgc
    19021 gaacacgtac aaacagcaat ggtgaggcgg catcaagagc agggatccgt catttatgcg
    19081 agcattttac cccaaaaacc ttttatttat aagggtcatt gcgaattatc tgatgcaaag
    19141 ttatgttatg ttaggcaaag taatcttcta ttattaataa gcacatcaaa acctttttga
    19201 atattaaata ataattaatt agcatcatcc tcattcatta attccgctta acaatagttt
    19261 cacaccttgc ccccattgcg acacctcgga aatcatcacg ctgttaaact ctccgtaatg
    19321 cggaaatatc atcatcgcgt ttcattagtg aattcttaaa tgaggcattt tcacacaatt
    19381 atcttacaga taaaaaaacc agacttgcaa ttaagaatca gaacaattca ctatataaca
    19441 ttgcatgcaa agcatataca cctcattatt ttgtcattat taagttatta acagcacaat
    19501 cgagccttcc cctctggcaa aatcttattc tgcagacctt caaaacaccg tcctggggga
    19561 gtacattgtt ctaagctgac ttccacggca gggagtggcg ataacagcaa aaaaggtcaa
    19621 gattcatgtc ccccattgaa aaatccagca aattagagaa tgtctgttat gacatccgtg
    19681 gtccggtgct gaaagaagca aaacgcctgg aagaagaagg taacaaggta ctgaaactga
    19741 acatcggcaa cccagcccca ttcggttttg acgcgccaga tgaaatcctc gttgacgtga
    19801 tacgcaacct gcctaccgct caagggtatt gcgattccaa agggctttac tccgcgcgta
    19861 aagccatcat gcagcactac caggctcgtg gcatgcgtga tgttaccgtg gaagatattt
    19921 acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg ctgaacagcg
    19981 gggacgaaat gttggttcct gcaccagatt acccactctg gaccgcggcg gtttcgcttt
    20041 ccagcggtaa agcggtgcat tatctttgcg atgaatcctc tgactggttc ccggacctcg
    20101 atgatattcg cgctaaaatt acgcctcgta cgcgtgggat cgttattatc aacccaaata
    20161 acccaaccgg cgcggtatat tccaaagagc ttttaatgga gattgtggag attgcacgtc
    20221 agcataatct cattatcttc gccgatgaaa tttatgacaa aattctctac gacgatgctg
    20281 agcatcactc aattgcgcca ctggcacctg acctgctgac cattaccttt aacggactgt
    20341 cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac gggccgaaaa
    20401 aacacgccaa aggctacatc gaaggtctgg aaatgctggc ttcaatgcgc ctgtgtgcta
    20461 acgttcctgc gcaacacgcc attcagaccg cgctgggtgg ttatcagagc atcagtgaat
    20521 ttattacccc tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa ctgatcaacg
    20581 atattccggg cgtttcctgc gtgaaacctc gtggtgcgct gtatatgttc ccgaaaatcg
    20641 acgccaaacg ctttaacatt cacgacgatc agaaaatggt gctggatttc ctgttgcagg
    20701 aaaaagttct gttggtgcaa gggacggcat tcaactggcc gtggccggat cacttccgca
    20761 ttgtcacgct accgcgtgtc gatgatatcg agctgtcttt gagcaagttc gcgcgtttcc
    20821 tttctggtta tcatcagctg taatcttaat ttcactgccg gagattgcat ccggcagcgt
    20881 tatcccgcca caatgacctg atgatgtcat catacgtaag gtcactatga aacagagcca
    20941 tttttttgcc catctctccc gcctgaaact cattaaccgc tggccgctaa tgcgcaacgt
    21001 gcggacggaa aatgtgtccg aacacagttt gcaggtagcg atggtcgccc atgcgctggc
    21061 agctatcaaa aatcgaaaat ttggcggtaa tgtcaacgcc gaacgtatcg ctttactggc
    21121 gatgtaccac gatgcctcag aagtgctcac cggcgatctc cctactccgg tgaaatactt
    21181 caattcgcaa atcgctcagg agtacaaggc tattgaaaaa atcgctcagc aaaaactggt
    21241 cgatatggtt ccggaagagc tgcgggatat ctttgcgccg ttaattgacg agcatgcata
    21301 tagcgatgaa gaaaaatcgc tggtgaaaca ggcagatgca ctgtgtgcat atctgaaatg
    21361 tctggaagaa ctcgcggccg gaaataatga attcttgctg gcaaaaacgc gactggaagc
    21421 gacgcttgaa gcgcgtcgca gccaggagat ggactacttc atggaagtat ttgttcccag
    21481 cttccatctt tcgctcgatg agattagcca ggattcaccg ctgtaagcag ccggagtctg
    21541 cgtcgcatca ggcaataagc gccggatgcg acatcaggct cttgtcaaaa cggaaacagc
    21601 atcgggatca tcaccacaca aaccgccatc acgataatgg tgaacggtac ccccaacttc
    21661 acaaagtcac tgaagctgta atttcccgga cctaaaacca gtgtgttaac aggtgaagaa
    21721 accggtgtca taaaggcggc ggatgctgcc atcgccacga ccatcgcgaa tggataaggc
    21781 gacaccccca tcgttttggc agcagccagc gcaatcggag ccatcaacac cgccgtcgcg
    21841 gtattagaga taaatagccc aataaccgcc gacaagacaa acaaacaccc cagcatcata
    21901 tgtggcccgt aaccaccgcc aatgtccatc agccctttca ccgccagcgc gacacctccc
    21961 gttttctgta atgccacagc aaacggcatc atcccaacga tcaaaataat gctcggccag
    22021 tgaatggatt tataggcgct ttcagcatct atacagcgga atttccccat cagcaggcag
    22081 gcgatgatag cggcaacagg attaggaatt tcatctgtca gcattaacgc caccattagc
    22141 accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg gcgatgcttc actcacctct
    22201 tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc gatcagtttc
    22261 cagttaccca caaccaggat gatatcgccc agcagcagag gctcatccgc cagcgaacct
    22321 tccagcgcca cgccattgcg ctttagcccc accacattca gtccgtagcg ggtacgaaaa
    22381 ccaatttcgc gcaccgattt accaatcagt tctgactcag gaattaatga aatctctgcc
    22441 atgcccacat caagggcctg gtcagaaaaa tactcgccgc gcagtaccat cggctccagc
    22501 aattgctcac tacaaaattg ccggagatcg acatcagccg cagacatatc aataagcaaa
    22561 acgtcacgcg cgcgaaattc agaaacccca ttaacgttca cgataacgcg acgaaaacgc
    22621 cgccagcgtt caacaccgat gacgttagcg ccataacgct cacgtaattt gagatcatcc
    22681 agccgttgac caatcattgg cgatccgggg cgaatagcca gacgtcgcgc acgcccggtc
    22741 agtcgatatt cacggataag atcgcgaaag gttcgacgcg tccagccttc gcgctgcggg
    22801 gtctgggtat cccctttcag catgaaacgc atcactaaca tatacaaaat acccagcacc
    22861 agcacgacca ggccaatagg tgttacgcta aagaaactga agccgtgata gccttcacgc
    22921 agcaattcac tgttgactac caggttcggc ggcgtcgcca ccagcgtcat catgccgctt
    22981 atcagcccgg caaaactcaa cggcatcatc agacgtgacg gcgaagtttg catacgcatg
    23041 gcaacgctta acaccacggg aataaagata gcgacaacgc cggttgaact cataaacgcg
    23101 ccaagccccg cgacggtcag catcaacaaa accaacattt tgatttcact gttgcccgca
    23161 actttgacca gccatgttcc cattacggtg gcaacaccgg tacggaccaa accatcgcca
    23221 ataataaaca aggcggcaat caggacaacg ttaggatcag aaaagccgga aaatacttcg
    23281 gggactgtca gcgttccgct taatgcaaac gcgacaataa caaacaaagc gaccgcatcc
    23341 atacgcactc tgcccgtcgc aaacaagaca atggcaacgg ccagtaatga aagaacccaa
    23401 atcaattcac cgttcacaac ttatccttgt taattgaggg agatgtcttg attctgccat
    23461 aaaaaagccc cgacgagacg gggctaaatc atgatcaggt gtttcactga ataataacat
    23521 cgccatttgg ctgtttggtc acagtaattt gctccagact gtggaggacc aaatcgacct
    23581 cattcaggcg cggggtatct gccggagcgt taaccgcaat gacatgacaa cccgccgcca
    23641 ggccagaaag cacgccagcg gagcatcttc caccaccaca cactcctgcg gcgcaagccc
    23701 cagcagctgc gcgcctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc
    23761 agcggttaca aacacctctg gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac
    23821 cggcatggag ccagaagtca caatggccca cggaatacct gctttattca aatgactgag
    23881 taaggcgatt gcccccggaa gcgggtaata ccttcggttt ccgtggcctc gatgtgctcc
    23941 agacgcgtaa actcggcggc aatatcagcc tcggatttgc ccgccataaa atggcgcaga
    24001 gaggtgatcg cctgtttacc gtgaatgaaa gccagcacct cttccggcgc taacccatga
    24061 cgtctggccc agttgctcca cgcccgttct accgcaggca gggaatccac cagcgttcca
    24121 tcaagatcaa acagaaaacc tttgcaccgc acgcgggcct cctcaggcat tgataatttg
    24181 attaatttcg ttggcgctca aatggtactg acgcgggcag gcatgccaca cattaagcat
    24241 gcgctgatat ttttcccaca ttggcgtctg ggcgttaaaa ccgtgagttc cggcgtcaaa
    24301 atgggtatag cgcccttcca cattaaccat aaagcggaca taaccgaggt aacgtgcttc
    24361 agtggcggcg tcaaagccga ggaaggtgac acgacgttca tcgatggatt gctggtcctg
    24421 caaattagac caggaaacat gcaacgcatg atacatctcc ataatgtcga tgatagtgcg
    24481 gcaggtttct tctttcagct cgccaaactc gcgatccaat tcacgcatct gtaatccgta
    24541 acctcgctca ataattgttt gcaggcgacg gtaacgttca gcatttgccg gatcgagcat
    24601 agtcatcatc ttgtactgat tagacaaaat aagacgttgc gcgttggtca tttccattgt
    24661 tgactcctgt atcactctac tacggtgaaa aaaaagaagg ctgagtatgc cttcttttat
    24721 atgcgtaatc aggggtcaat tacaaatcat caaggaaagt tttatccagt tgtttgaagg
    24781 cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt gccttcaacg ggtgccaaca
    24841 cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa ccagttaagg attgcagggg
    24901 gtaatggcgt tacagaacgc ttgcccagcc accacaatcc ctgcatgggt aaacttaagg
    24961 cgaacagtgc agtggcaact gccggcccaa gctgaccgcc cagggcaatc tgccagcaga
    25021 gagtaaatac ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc ttgataacgc
    25081 gattttcgac aaagaccggg gcaaggcgtt tttccagtgg ccacgtcttt gagtaatgct
    25141 gtccgcggcg aaacaagcta aaaaaattaa cagaacgatt atccggcgtt gacatgcttc
    25201 acctcaactt cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa
    25261 cgttcaaaac attttgtctt ccatacccac tatcaggtat cctttagcag cctgaaggcc
    25321 taagtagtac atattcattg agtcgtcaaa ttcatataca ttatgccatt ggctgaaaat
    25381 tacgcaaaat ggcatagact caagatattt cttccatcat gcaaaaaaaa tttgcagtgc
    25441 atgatgttaa tcataaatgt cggtgtcatc atgcgctacg ctctatggct ccctgacgtt
    25501 tttttagcca cgtatcaatt ataggtactt ccatgtcgag taagttagta ctggttctga
    25561 actgcggtag ttcttcactg aaatttgcca tcatcgatgc agtaaatggt gaagagtacc
    25621 tttctggttt agccgaatgt ttccacctgc ctgaagcacg tatcaaatgg aaaatggacg
    25681 gcaataaaca ggaagcggct ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta
    25741 tcgttaatac tattctggca caaaaaccag aactgtctgc gcagctgact gctatcggtc
    25801 accgtatcgt acacggcggc gaaaagtata ccagctccgt agtgatcgat gagtctgtta
    25861 ttcagggtat caaagatgca gcttcttttg caccgctgca caacccggct cacctgatcg
    25921 gtatcgaaga agctctgaaa tctttcccac agctgaaaga caaaaacgtt gctgtatttg
    25981 acaccgcgtt ccaccagact atgccggaag agtcttacct ctacgccctg ccttacaacc
    26041 tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa
    26101 cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga actgaacatc atcacctgcc
    26161 acctgggcaa cggtggttcc gtttctgcta tccgcaacgg taaatgcgtt gacacctcta
    26221 tgggcctgac cccgctggaa ggtctggtca tgggtacccg ttctggtgat atcgatccgg
    26281 cgatcatctt ccacctgcac gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc
    26341 tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac cagcgactgc cgctatgttg
    26401 aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc
    26461 tggcgaaata catcggtgcc tacactgcgc tgatggatgg tcgtctggac gctgttgtat
    26521 tcactggtgg tatcggtgaa aatgccgcga tggttcgtga actgtctctg ggcaaactgg
    26581 gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg
    26641 gtttcatcaa caaagaaggt acccgtcctg cggtggttat cccaaccaac gaagaactgg
    26701 ttatcgcgca agacgcgagc cgcctgactg cctgatttca caccgccagc tcagctggcg
    26761 gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat
    26821 tattatgctg atccctaccg gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat
    26881 ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg
    26941 taccggtggc gatgcgcccg atcagactac gactatcgtg cgtgcgaact cttccaccac
    27001 gacggccgct gaaccgctga aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa
    27061 agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt
    27121 cgttctggtt gaaggtctgg tcccgacacg taagcaccag tttgcccagt ctctgaacta
    27181 cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt atgtctcagg gcactgacac
    27241 cccggaacag ctgaaagagc gtatcgaact gacccgcaac agcttcggcg gtgccaaaaa
    27301 caccaacatc accggcgtta tcgttaacaa actgaacgca ccggttgatg aacagggtcg
    27361 tactcgcccg gatctgtccg agattttcga cgactcttcc aaagctaaag taaacaatgt
    27421 tgatccggcg aagctgcaag aatccagccc tctgccggtt ctcggcgctg tgccgtggag
    27481 ctttgacctg atcgcgactc gtgcgatcga tatggctcgc cacctgaatg cgaccatcat
    27541 caacgaaggc gacatcaata ctcgccgcgt taaatccgtc actttctgcg cacgcagcat
    27601 tccgcacatg ctggagcact tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc
    27661 tgacgtgctg gtggccgctt gcctggcagc catgaacggc gtagaaatcg gtgccctgct
    27721 gctgactggc ggctacgaaa tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc
    27781 taccggcctg ccggtattta tggtgaacac caacacctgg cagacctctc tgagcctgca
    27841 gagcttcaac ctggaagttc cggttgacga tcacgaacgt atcgagaaag ttcaggaata
    27901 cgttgctaac tacatcaacg ctgactggat cgaatctctg actgccactt ctgagcgcag
    27961 ccgtcgtctg tctccgcctg cgttccgtta tcagctgact gaacttgcgc gcaaagcggg
    28021 caaacgtatc gtactgccgg aaggtgacga accgcgtacc gttaaagcag ccgctatctg
    28081 tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt
    28141 tgcagcgtct cagggtgtag aactgggtgc agggattgaa atcgttgatc cagaagtggt
    28201 tcgcgaaagc tatgttggtc gtctggtcga actgcgtaag aacaaaggca tgaccgaaac
    28261 cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt acgctgatgc tggaacagga
    28321 tgaagttgat ggtctggttt ccggtgctgt tcacactacc gcaaacacca tccgtccgcc
    28381 gctgcagctg atcaaaactg caccgggcag ctccctggta tcttccgtgt tcttcatgct
    28441 gctgccggaa caggtttacg tttacggtga ctgtgcgatc aacccggatc cgaccgctga
    28501 acagctggca gaaatcgcga ttcagtccgc tgattccgct gcggccttcg gtatcgaacc
    28561 gcgcgttgct atgctctcct actccaccgg tacttctggt gcaggtagcg acgtagaaaa
    28621 agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct gacctgatga tcgacggtcc
    28681 gctgcagtac gacgctgcgg taatggctga cgttgcgaaa tccaaagcgc cgaactctcc
    28741 ggttgcaggt cgcgctaccg tgtttatctt cccggatctg aacaccggta acaccaccta
    28801 caaagcggta cagcgttctg ccgacctgat ctccatcggg ccgatgctgc agggtatgcg
    28861 caagccggtt aacgacctgt cccgtggcgc actggttgac gatatcgtct acaccatcgc
    28921 gctgactgcg attcagtctg cacagcagca gtaacctcgt catcatccgc agctttgcgc
    28981 tgcggatatc tgaaccggaa ataatcacta tttccggttt tttattctct taatctgcat
    29041 taatcctttc tgattatctt gcttaactgc gctgcatcaa tgaattgcgc catttcactt
    29101 tacatactta ccactttgtt ttgtgcaagg gaatatttgc gctatgtccg caatcactga
    29161 atccaaacca acaagaagat gggcaatgcc cgatacgttg gtgattatct tttttgttgc
    29221 tattttaacc agccttgcca cctgggtagt tccggtgggg atgtttgaca gtcaggaagt
    29281 gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc gtagatccac actcatttcg
    29341 cattctgact aacgaagcag gcgaacctga gtatcaccgc gtacagctgt tcacgacggg
    29401 cgatgaacgc ccgggcctga tgaacttccc gtttgaagga ttaacctcag gatcgaaata
    29461 cgggacagct gttggcatca tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt
    29521 gatgcgtaca ggaaccattg ataacggtat cctggcgctt attcgccata cccgcgggaa
    29581 tgaaattctc tttattcctg cgctgtttat tctgttttca cttggcggcg cggtatttgg
    29641 tatgggagaa gaggccgtcg cctttgccat tatcatcgca ccgctaatgg tccggctggg
    29701 ctatgacagt attaccaccg tcctggtgac ctatattgcc acgcaaatcg gttttgccag
    29761 ttcgtggatg aacccgtttt gtgtggtcgt tgctcagggg attgccggcg ttccggtgct
    29821 ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc actatgattg gcctgatctt
    29881 taccatggtg tacgcctcac gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc
    29941 cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa cgtccgttta cctttggtga
    30001 ctggctggta ttgattgtcc tgaccgccgt aatggtctgg gtgatttggg gcgtgatcgt
    30061 taatgcctgg tttattccag aaattgccag ccagttcttc accatgggtc tggtgattgg
    30121 catcatcggc gttgttttcc gccttaacgg catgacggtt aataccatgg cttcatcctt
    30181 taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg gtgggtttcg ccaaagggat
    30241 tttgctgctg gtcggtaatg gtgaagcggg tgatgccagc gtgttaaata ccatcctcaa
    30301 cagcattgcc aatgccatta gcggtctgga caacgcggtc gcggcctggt ttatgttgct
    30361 cttccaggcg gtatttaatt tcttcgtgac gtccggttct ggtcaggcgg cgttaaccat
    30421 gccgttactg gcaccgcttg gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc
    30481 attccagttt ggtgatggct tcagtcacat catttaccca acctcagctt cgttaatggc
    30541 aactctcggt gtgtgcaggg tggacttccg taactggctg aaggtgggcg cgaccctact
    30601 tggactgctg tttattatgt ccagcgtcgt agtgatcggc gctcagttga tgggctacca
    30661 ctaaaaatgt taagagccgc aattgcggct cttttttcat tctgccgttt cagtctctac
    30721 ggcttcattt ttggcattgc gcttcatcca caacgccagc gctttcagcg agtctggagt
    30781 gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc cagcaaactt cactgacttc
    30841 atcttcctgt agggcgaagg gaccgtgaga gacgcagctg aacaacgcgc cccagacacg
    30901 gcaattttta tcttcgaaat agaactgccc gtgctcggca aaggggacac cggcaatgcc
    30961 caactcttct tccgcttcgc gacgcgcgga ttccagcagt tgctcatcgg cctgaaccac
    31021 accgcctgcg gtcgcatcta acatgccggg taaaaagtct tttgtctcgg tacgacgctg
    31081 gaccagaatt ttgcccatgc catcatgcac gacgatgtaa gttgcacgat gacgcagaca
    31141 ctgtgcccgc atttgttccc ggctggcttg tgcaatgact tcgttctctt cattgacaat
    31201 atccacccat tcagtacttg ccaaacgacg ctgttccatt atcaggaaac cttctttttc
    31261 tggcgctctt acggcgcatt ttgaattgtg ggtaaattac ggattaatcg cgacctgcgc
    31321 aatgatactt tgatcattga gtgcgattac actaagtacg tcattatcca gcatgccata
    31381 actcgccgga ttaccgcctt tcggaatact caccgagccg gggttgaagt ggaaaatctc
    31441 cccccgctgt tctgccactg gcagatgggt atgaccgtac accagcacat cgttctggtt
    31501 taaagcaggt agattttccg ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt
    31561 ttccagtaat acctgttgcc acggcgcggt tatcgggaaa tgcagcagca tttgatccac
    31621 ttcgctgtcg cagttgccgc gcacagcgat aaccttatgt gccacttcat taagccgttc
    31681 agcgactttg gctggcgcgt aaccctccgg taaagcatta cgcgggccgt gattcaacac
    31741 gtcgccaagg atcaccagcc actgggcacc gctttgggca aacaactcca gaacacgttc
    31801 cgtcgccggt aacgacccat gaatgtccga tgcaaacatc agtttcatca ctcactcctc
    31861 gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg ctatcagcca gattgcttcg
    31921 ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca ctggaatgtc gcataatcca
    31981 ccaggcgttc tggcacctca tcgccatgta gtaccaggcg gttaatcatt agcgccagat
    32041 cagtatcagc aatgcaccat tcaccaaata aattcggctg acccagtgct aacaaatgtt
    32101 ctgccatcgc gaacagtttc tctgcactgg cttttccctc ggccgttagt ggcgcttttt
    32161 tcgcccccgc aaagacaaca tccgtcggac gctcttcgcg gatgggcatc agatcgctgc
    32221 gcagccaggc ctgaatccgt cgtgcacgcg cacgattttc taaatcaagc ggataaatac
    32281 gttcccaggt cggtggcgca aatcgatctt ccagatactc cgcaatggca gaagattcac
    32341 tcaactcaaa atcatcgatt tgtaataacg gcacacggcg tgtctgaccg taaccttgcc
    32401 acgtcggctt caaatgttca ccgctgtcga ggtcgatggt cttgatatga aacgacaggc
    32461 ctttttcctg caacgccacc caggcggata acacataagg ggagaaaaag tgggcatctg
    32521 accaaagcgt gatagcgggt ttactcatta tgtcctcggc aataaagggg gctggctatc
    32581 aaactataga gcctttttgt cactgtcacg tgacgaatac tcacgtaagt cgccgcgctt
    32641 tctatacttg tggtgttcag atgattaaac aaccggagct gcaatgatcg atctctattt
    32701 cgccccgaca cccaatggtc acaaaattac gctgtttctc gaagaagcag agcttgatta
    32761 tcgcttgatt aaggtagacc tggggaaagg cggtcagttt cgtccggaat ttttgcgcat
    32821 ttcgcccaac aacaaaattc cggcaattgt tgatcattct cctgccgatg gcggcgaacc
    32881 gctaagcctc tttgagtctg gtgccatttt gttgtatctg gctgagaaaa caggactctt
    32941 tttgagtcat gaaacgcgtg agcgcgccgc cacattacag tggttattct ggcaggtagg
    33001 cggactgggg ccgatgcttg ggcaaaatca tcattttaat cacgcagccc cccaaaccat
    33061 tccttacgct attgaacgtt atcaggttga aactcagcgt ctttaccatg tactaaacaa
    33121 gcggctggaa aactcgccct ggctgggagg cgagaactac agcattgcgg atattgcctg
    33181 ctggccgtgg gttaatgcct ggactcgcca gcgaattgac ctcgcaatgt atccggcagt
    33241 caagaactgg catgagcgga tccgttcgcg ccctgccacc gggcaggcac tgctaaaagc
    33301 acaactcggt gatgagcgtt cggatagtta acagaaacag gttctcgtgt attatttcat
    33361 cctaagtaaa acaacggaga acctgcaatg gcacaacctg ccgctattat tcgtataaag
    33421 aaccttcgtt tacgtacgtt tatcggaatt aaggaagaag aaattaacaa ccgtcaggat
    33481 attgttatca atgtgacgat ccactacccc gccgataaag cgcgcactag cgaagatatc
    33541 aacgatgcgc tgaattatcg caccgtaacg aaaaacatta ttcagcatgt agagaataac
    33601 cgtttctctt tgttggaaaa attaactcag gatgtgctcg atatcgcacg tgaacatcac
    33661 tgggtgacgt atgctgaagt ggagatcgat aaactgcacg cgctgcgcta cgccgattcg
    33721 gtatccatga ccttaagctg gcagcgttaa ccgccatatc gggaggctgc atgaacatag
    33781 tgatcaccgg agggacggga ttaattggtc gccatttgat tccgcgtttg ctggagctgg
    33841 gccatcaaat cacggtagtg acgcgtaacc cacagaaagc cagttccgtt ctcggccctc
    33901 gggtgacgct atggcaaggg ctagccgatc aaagcaacct caacggcgtt gatgcggtaa
    33961 tcaacctggc cggagaaccg attgctgata aacgctggac tcacgagcaa aaagagcgtc
    34021 tctgccaaag ccgctggaat atcacgcaaa aactggtcga tttgattaat gccagcgaca
    34081 cgccgccgtc ggtactcatt tccggctcgg caacgggcta ttatggcgac ttaggtgaag
    34141 tggtggttac cgaagaggaa ccgccgcata acgaatttac tcataaactc tgcgcccgct
    34201 gggaagagat tgcctgccgg gcgcaaagtg acaaaacgcg agtgtgcctg ctgcgaaccg
    34261 gcgtggtact ggcaccggac ggcggtattc tcggtaaaat gctgccaccg tttcgtcttg
    34321 gcctgggcgg gccgattggt tccggtcggc agtatctggc ctggattcat attgatgata
    34381 tggtcaacgg cattctctgg ctgctggata acgacctgcg cgggccattt aatatggttt
    34441 cgccctaccc ggtacgcaat gaacaatttg cccatgcgct cggtcaagcg ctgcatcgcc
    34501 cggccatttt gcgcgtcccc gcaaccgcca ttcggctgtt aatgggcgaa tcttcaatac
    34561 tggtattagg tggacaacgc gcgctgccta aacggctgga agaagcgggt tttgcgtttc
    34621 gctggtacga tttagaagag gcgctggcgg atgtcgttcg ctgatgtggt ttacagcaaa
    34681 catccgccag ttaactcccg gtgttacagg attagtggct ttgcgcgata agatcgtctg
    34741 gtgaaagtcg ggtcaccatc ataactaact ctctgtctaa acctctatcc agcatctcct
    34801 gagcaatacg cagggcttct tcgtgtttgc cctgcattgc gccttcttca cgtaatctgt
    34861 cagcaatggt catcagtttc tccttttctt gtggtgcgcg ttctgttatc tcaccaataa
    34921 atgcacggaa acgctgggca tcccctgttt gtaatacgta attaaacagg gcttttagct
    34981 gtctgtcatt agtgttccct gtaactagca gcgaaacaat ttggtcgact aatcccaaca
    35041 gatcgcgctg acgaatatgt ttctgaatta actccaacag cgccattttg cggtgttgca
    35101 taatctcgtc atccggcacc acggtaatat ccaccaacgg aaacgctgat gaatatattt
    35161 tgcgggctat agcaggctcg gcaaattcat caagccagca gagtgaataa ggataaggac
    35221 ttctgcaacc atgataaaac agcatcggga tcaccaatgg aagctcttta tagcccgcat
    35281 caagatggtt ttgcattgcc gcaatggaat aacgcatcat gcgaaaagcc attaattctt
    35341 ccagcttact ttggtgctct atcactacat aaatataacc cactccctcc tgcgttttca
    35401 cagaccacaa gaggtcggaa taatattgcc gcaggtcttc atcaataaaa ctgtttgatt
    35461 ctagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg cgcgggaaga tgaatatcaa
    35521 taaaatcccg cgcggtgtct ggatggcgta aaaaagattt aaataccgca tcatgcggcg
    35581 tggaagttgt cgatattgtc atggcgttcc gtcacccctc aaatttaaga tgacgcgaca
    35641 ataaccacga ttaacaccca atgcagcagg cagtttttat ctttggacag cgaattcaga
    35701 agtattttta gcggactgca acttttacat ttcagcggaa gctgactcgc aaattcccca
    35761 tgaaaatact ccggcagcgt tagccaccgg agaggaaatt atttcagcga tcccttaagg
    35821 aactgttgca gacgagggct ttgcgggttg ccaaataact gctccggcgc gccctcttct
    35881 tctattttcc cctgatggag gaaaatgacg tgagtagaaa catggcgagc aaagcccatt
    35941 tcgtgagtca ctaccaccat ggttttcccc tcttctgcca gttgctgcat aatacgtaac
    36001 acttcgccta ccagttcagg atcgagcgcc gaggtaggtt catcaaacag taaaacttcc
    36061 ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt gctgaccgcc ggaaagatgc
    36121 accggatatt tcccctgcgc acgttcgtct atcccgactt tcgccagata cttcaccgcc
    36181 cgctcgcgcg cttcctgctt gctcaggccc aacacctgaa tcggcgcttc catgacgttt
    36241 tccagcaccg tcatatggct ccagagattg aagtgctgga ataccatcgt caggcgtgtg
    36301 cgcagtaagc gcagttgatt tttatcggcg actttgagtt gaccgtcttt gtcgcgcacc
    36361 agattgatcg tctggccatt gaccacgatc gacccttcac tcggtttttc gaggaagtta
    36421 atgcagcgca gaaaggtact tttccccgat cccgacgatc cgatgatgct tattacatct
    36481 ccggcattcg cttgcagtga gacccctttc agcacttcat gttcgccgta gcgtttgtgc
    36541 aaatcgataa cgtttaattt attctcggac atcgtattct cagtgcgttg aagaaggttt
    36601 cacatgctgc aaccagcgtt tttccgctct gcgaaagagg ctgatcagga cataagagat
    36661 gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa ggttgatacg tggcggcgtt
    36721 aatatcgcgg gctattttca gcagatccgg cacagtggcc gtaaacgcca gcgcagtaga
    36781 gtgcagcatc aggatcactt cgttgctgta tgccggtaac gcaatacgca gcgcagaagg
    36841 caaaataatg caacgataca ttttaaaagt cgagaagcca taggctctgg cggcttcaat
    36901 ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc tcggtagtgt aagcgcaggt
    36961 gttaagcgtc agcgccagca cggtacagtt caggccactg cggaagaaag cgttaaggaa
    37021 ttcggttccc ttaacaatct caagcgtgta catgccggaa tagaacacca gcaactgaac
    37081 atacagcggc gtaccgcgaa aaatataggt aaataaccag attggaaact ggatgtattt
    37141 attactggag acacgaccaa tcgccagaaa cagcgccagg actccgccta tcactaccga
    37201 caaaataagc agccacagag tgatcgccac accagtaaag cgataaccgt cggtccacag
    37261 cagcggtttc cagtattcat gtaagatttc gatcacaggt cagccctctt cacacccacg
    37321 gagtagcggc gctcaaggaa cagcagcaca ccattggaaa cagtggtgaa aaccaggtaa
    37381 atcacgccac agacgatggc gaaatagaac ggttcccagg tacttttgcc tgccagttgg
    37441 gtggctttga ccacatcttc caggccgagt aacgaaacca gtgcggtaga tttgaggatc
    37501 acctgccagt tgttgccaat gcctggcagg gcgtaacgca tcatcgccgg aaacatgatc
    37561 cgccgaaaca cttgcccacg agtaaaaccg aacgccgtcg ccgcctctat atgtcctttc
    37621 ggcactgcca taaaagcacc acggaacgtt tcggtaaaat aagcaccgta gataaaaccg
    37681 agagtgataa taccagcgac catcggatcg atatcaatct gcccgacgcc catcgcctcc
    37741 gtcaccgtgt ttagcgcaat ctgcaaaccg tagaaaatca gcagcatcaa cactaagtcc
    37801 ggcacgccac gaatcagcgt ggtgtaccct tcgaaaatca gccccgaaag ccgattttgc
    37861 gagagcttac cgccagcgcc aattaaaccg atgattacag cgagcactac agagctgata
    37921 gccagctcca gcgtgacgag cgcaccctgt aaaataacac ctgaaaaccc atacaacatg
    37981 ctgcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt ctccctccgg ttacccgaag
    38041 ggagcgggct ttctttcacc gatggcgatt agccaccata aacatcaaaa tcgaagtact
    38101 ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc ggcaaaggct ttgttcagtg
    38161 cttcgcgcag ttcgttatct tctttacgca ggcccatgcc ggtccctacg ccaaacagtt
    38221 tttcatcttt aacagacggg ccaccgaatt tgtaatcttt accgacaggt tgtttgagga
    38281 aaccttcgct ggcagcgacc tcatcctgga acgcggcatc aatacgtccg gcagtcaggt
    38341 cagaataaat gttgtcctgc ccctgatacg agacgatttc aatgcctttt ggtgcccaat
    38401 gttcattacc gaacgtctcc tgggtggtgc cctgcaatac gcctacccgt ttgcctttca
    38461 gcgactcgac tgtcggctga atgtcagaat ttttcgccac caccaaacga gaatcggcag
    38521 cgtacagttt gtcggtgaag gctatttctt gctgacgttt ttccgtaatg gaaagcgatg
    38581 acatgatggc gtcaatcttc ttcgctttta aggacgggat taacgcatcc agcggatttt
    38641 cgacaaacgt acattgcgta ttgatgcgtt tgcataattc ctttgccaga tcgatatcga
    38701 agccaaccag ttcgccttgt gaattttttg attcaaatgg cgcataggtc gggtcggtac
    38761 cgatgcggat gttttgcgga atcgcagcaa atgccgcagt tgcgctggag aaggccagaa
    38821 ccagagagag cgatagcacc agttttttca taatgtcctc aactgacagt ctttttatag
    38881 ggaatattta caggtttgtt acacttatcg tgccataaaa ttgaccatca aggcaaaata
    38941 ttccgccccg ttagacgtat tatttttcat taaaaatgct taagtatgca tttcaatgca
    39001 ccacgatagt gcaacaaacc tagcgtgcac catgatgatc attccatcag gtacagcttc
    39061 ccagcgacgt atcagtcacc gtagacatta aagtcgaaat actttttcgc catcttgtcg
    39121 taggtgccgt cctgacgcag ctcgccaagc gccttattga aggcagccgt cagttcagca
    39181 tcatctttac gtagccctac accggtgcca tcaccgaagt attttttgtc ttttactgat
    39241 gagccagcaa aggcgaaagc tttaccagca ggttgcttga ggaatccttc gctggcagca
    39301 acttcatctt gtaacgcagc atccagacgt cctgcagcca gatcggaata gaccaaatcc
    39361 tggttggcat aggccaccac atccacgcct ttgctacgcc aggtctcgtt agcgtaagct
    39421 tcctgggttg atccctgcag cacaccaaca tgtttacctt tcagtgaatc cagcgttggc
    39481 tgaatcggtg aacctttggc agcaatcaaa cgagaatctg cggcgtacag cttgtcggag
    39541 aaggcaatct cctgctgacg tttatcggta atggaaagcg acgaaataat ggcgtcgatt
    39601 tttttcgctt tcagtgaggg gatcagcgcg tcaaagtcac tggcaaccca ggtacatttc
    39661 acctgcatcc gtttgcacat ctcgttaccg agatcgatat caaagccaac aaaatcacct
    39721 ttagcatctt tcgacgagaa cggcgcatag gtggtatcgg ttccgatacg taccgtctcc
    39781 ggtagcgccg catagctgga agccgctgcg gagagaccga ctaacaaaga cagagcgaga
    39841 atcgacttct tcatacataa ccctcaagtg aaaatggctt ttttatgttg tgtattgtgt
    39901 tgtgtgtttg caggctcttt catgcaggtc ttatgccatc ttgacgacaa cagtaacatt
    39961 caacgttaaa tatgttaata agacgttgca ttattgtcct gaagttgaag atagcaggta
    40021 tagcggttga atcgcagcgt ttcgattgaa tggcagaaac aaaatgtcga ggatttgatc
    40081 gcggttgcaa aattgccctg aaacagggca acagcggagt tatgcgccct gccagcgggc
    40141 aaagagatct tcaggaaggg ttattgcaaa ctggtcaaga acacgattaa ccgtctgatt
    40201 tatcacatca tcaagggatt gcgggcgatg ataaaacgcc ggaacgggag gcataatcac
    40261 cgcaccgatt tctgccgcct gagtcattaa acgcagatgg cctaagtgca atggtgtttc
    40321 acgcacgcag agcaccaacg ggcgacgctc tttcagcacc acatctgccg cacgggtcag
    40381 taagccatca gtatagctat ggacaatgcc ggaaagggtt ttgattgaac agggtaaaat
    40441 caccatcccc agcgtctgga aagaaccgga agagatgctg gcggaaatat cgcgcgcatc
    40501 gtgcgtgaca tcagctaatg cctgcacttc gcgcagggaa aaatccgttt cgagggataa
    40561 ggtctggcgc gccgcctggc tcatcaccag atgcgtttcg atatctgtga catcgcgcag
    40621 aacctgtaat aagcgcacgc cataaatcgc gccgctggca ccgctgatgc ctacaatgag
    40681 tcgtttcata aatagttgcc ctgtcagact tgcaggcaga ctttgcagta tttcggcggg
    40741 agttgcaagt cagggtgcca gaccggcacc ctcagcgaag gcatcatcct tcgttatgca
    40801 tttcgagatt ttccacttcg ttctgacgtt gcactgcttt ggcgtcatca ttacgtaacg
    40861 tatcgaggaa atcgaggtag ccctgatcaa catctttggt gacgtagacg ccgttgaaca
    40921 ccgagcattc aaactgctgg atatccggat tttcagcgcg aacggcgtcg atcagatcgt
    40981 tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg gcgaatttca tcaacttcgc
    41041 gaccgtgagc gatcagttcc gtggcgctcg gcatatcaat accataaacg ttcgggaagc
    41101 gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc tccggcttcg cgtgccatct
    41161 cgataatctg ctcagaagtg gtgccacgga cgatggagtc gtcgaccagc aggacgtttt
    41221 tatcgcggaa ctcggcgcgg ttggcattca gtttacggcg cacggactta cgacgcagct
    41281 gctggcccgg catgataaag gtgcggccaa catagcggtt tttaacgaag ccctggcggt
    41341 acggtttgcc cagaatacga gcaatttcca gcgcgatatc acacgaggtt tctgggatcg
    41401 ggatcaccac gtcgatatcc agatcttccc attcgcgggc aattttctcg cccagtttcg
    41461 tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc gatgaacgag tccgggcgag
    41521 caaagtatac atactcaaac aggcacggat tgctgaccgg attgtcagca cattgacggg
    41581 taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc cggcgcgacg tcacgcagga
    41641 aatcaaagcc cagcgtatcg agcgctacgc tttcggaagc gaccatatat tctgtacggt
    41701 tctcgtcaat atcacgtttt cccagtacca gcggacgaat cccgtttgga tcgcggaaag
    41761 caaccatacc gtggccgata atcatcgcca cacaggcata tgcgccgcgg attaagcggt
    41821 ttgtggcagc aatggcagcg aaaatattgt cggcttccag cgggtagtgg cggaagttgt
    41881 ccagctcgct agcgaagata ttaagcagaa tttccgagtc ggaagtggtg ttgatgtggc
    41941 ggcgtttttc ttcaaacagt tttttacgca actcgtgagc gttggtcaga ttgccgttgt
    42001 gggcaagcgt aatgccatac ggggagttaa cgtaaaacgg ctgcgcttca gaggcgctgg
    42061 agctgccagc cgtagggtaa cgcacatgac caatgcccat attgccctgc aaacgctgca
    42121 tatggcgagc ttcaaataca tcgctcacca gcccgttcgc tttacgcaaa cggaagcagt
    42181 tattggcatc tatggtgatg atgccggcgg catcctgacc gcgatgctga agcaccgtta
    42241 aggcatcata aatcgactgg ttaaccggca taacaccggc gataccgaca ataccgcaca
    42301 tacgtctttt tcctcgttaa gccacatctc agagcactta cgctctgggc aagaaacttg
    42361 acgagctttg cagataatca aaaaaccatc tgatgataaa actgaactgc gggatcagct
    42421 gtgatttgct ccagtcttcg cttttcgaca ccccggtaaa ggagtcgaga aagaagagaa
    42481 tggcggcaac aatcaacaca ccgcgcaacg caccgaaaca gacgcccagc acccgatcgg
    42541 tgcctgacaa ccccgttttc tccaccaact ggcctatcac gaagttcacg atagcaccaa
    42601 caatcagggt agcgataaac agtaccgcga tggcaattcc atttcgaacc agttcgtctt
    42661 caaagcccgt aaaccagact gacaggtaag tgtagtaatg actggcaaca aagaaagcac
    42721 aaccccatgt caccagcgat aacgcttcac gaacaaagcc gcggatcagg ctaaccagag
    42781 aggaaaaagc aatcaccgcg attatggcgt aatcaatcca gaccatatgt gtcccacgat
    42841 tttacgccct gtcatcctgt tcggggcgca ttctaacaga aaaagaaaac gtttgcgtag
    42901 ggatttcctt cccacgcatc aataaaaatg gcgctgaaaa aatattcaac gccatcgact
    42961 ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc ggcgtaaacg ccttatccga
    43021 cctacggttc taacactgcc caggcctgat aagacgcgcc agcgtcgcat caggcaagac
    43081 tgtattaatt cggcgtatag cccattacca cgccacttaa gccagaaagt tgcttcaact
    43141 cacccagcga acctttcagc ttatctttcg aggcatccgg cccaaccaga atacgggtaa
    43201 ttttaccctg cactggcgtg gatggcgacg tataaacccg ataaccggca ccgcgcagct
    43261 taccgacaat ctcattcact ttatcggcat ttttcagcgc acccagttgc acaacatagg
    43321 ctttacccgt tggcgctgct ttttcttcca cgaccggctt cggttctggc ttcggcgcag
    43381 gtggtgcttc aaccttcggt ttaggcggct ccaccggttt cggcttcggt ggggcgaccg
    43441 gtgcaggttc cggttcaaac tcggtgttat tggctgcaat agtggccgga tcgagcgacg
    43501 gtgctgcggc atcacctgcc cgcacctctt ccgctgcgcc ttccggcggc tgcgtcggca
    43561 acgcctgggt ggcggctggc atcatatcag gctcatcgcg atcgcccgct tttggaacca
    43621 gcgggatagc cgcaaactca tcctgataat gttttttctg cccgtccagc agccctggaa
    43681 gtacaatcac ccccagcgcc accagcacga tcgtgcccac taaccgattc tgaaacttac
    43741 ttgccaccgc ttctcctcgc gtcaatcact tccatgacat gtgcgaccgt gtggaaagaa
    43801 ccacacacca gcacggtgtc ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc
    43861 gcaacgctat caaatgattt gccgttaccc aaatgctcaa gcagttgttc tgccgtggca
    43921 ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa ccacgctttt caaccaggcc
    43981 agagttccgg caatatcttt atcatgtagc ataccgataa ccgccagcac gcgcccgttt
    44041 ttcggtagcg ctttcatacg cccggtgaga tattccgccg catgtggatt atgcgcgaca
    44101 tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac gtcccggcaa aattgcgctg
    44161 gcaatcccgt cgcgaatggc attttcactg acttccagcc cgctggcacg cagtgccgcc
    44221 agcgctgttg cggcattcgg ttgcgggaca agcggcaacg gcagattttc cagcgtgccg
    44281 tgagcatcgc taaacgccca gtcatgatcg gtgacggaat agttccactc aacgccccga
    44341 cgttgtaaca gtgcaccttt ttcctgcgcc acatcagcaa tggtagaagg catttccggc
    44401 tcaccgacaa ttgccggttt tgcgctgcga aagatgcctg ctttctcgcg accaatactt
    44461 tcgcgatctg gtcccagcca gtcggtatga tccagcgcaa tgctggttac tacagccaca
    44521 tcggcatcga caatattggt tgcgtccaga cgaccgccca gccctacttc cagaatcact
    44581 acgtcaagtt gtgcctgttt gaacagccac aacgccgaca gcgtaccgta ctcgaaatag
    44641 gtcagggaaa tatcaccgcg tgccgattca atctccgcaa aagaggcggt gtgggccgat
    44701 tccggcaatt cctgcccctg cacacgtacg cgctcggtat aacgcaccag atgcggtgaa
    44761 ctgtagacgc ccactttgta ccctgccgcc atcagaatcg actccagcgt acggcaggtg
    44821 gtgcctttgc cattcgtacc cgcaacggta aacacaaatg gcgctggttt caggacgcca
    44881 agacgcgccg cgacctggct cacgcgctca aggccgagat cgatagtttt actgtgcagg
    44941 ttttccagat aagaaagcca cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata
    45001 atcatggtat ccgctgattc gttacggtga gaatagcaaa agggcagagc cagtggccct
    45061 gcccttatca gttatcaggc ctcaggttcc tgatccggta ccgggggtac cactacgcct
    45121 tcacgcggcg cttcaggatt cggcgctggc agattcatca acttcgccag aatgctcgcc
    45181 agtttcaggc gcatttccgg acgatggacg atcatgtcga tggcgccttt ctcgatcagg
    45241 aattcactgc gctggaatcc tggcggcagt ttttcgcgaa cggtctgttc gataacacgc
    45301 ggaccggcaa agccaattaa cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg
    45361 aaacttgcag aaacaccgcc catcgtcggg tcggtcagca cggagatgta cggcaagccg
    45421 cgctcctgca tttttgccag tgccgcagag gttttcgcca tctgcatcag cgacatcagt
    45481 gcttcctgca tacgtgcgcc accagaggcg gagaagcaga tcagcgggca gttatcttcc
    45541 agcgcctgct caacggcacg cacgaaacgt gcacccacaa cagaccccat tgaaccgccc
    45601 ataaaggcga actcgaatgc cgcagcgaca accggcattc catacagagt gcctttcatc
    45661 accaccagcg catctttttc gccggtttct ttctgcgcag aagccagacg gtctttatac
    45721 ttcttggagt cacgaaactt cagcacatct ttcggctcaa gctcgctacc cagctccaca
    45781 aggcttcctt catctaacag gctatgcagg cgattacgcg ctgtcatacg catgtgatgg
    45841 tcacacttcg gacagacctc aagattacgt tccagctcag cgcggtataa aacctgaccg
    45901 cagctatcac acttagtcca caccccttca ggaatgctcg ccttgcgggt gggagtaatg
    45961 ttgcttttaa ttcgttcaat ccagctcatt agggaccttt ctgtctgaac ctggttcgat
    46021 gccagtttta tctttgggga cgcataatgc catttttgcc cccaacagac catgaatgtt
    46081 gcacattaaa acataacagc ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta
    46141 ctttttattt tgcggcgcgt gccgcagcgc gtttgtgacg aataaattcg aaaaccccag
    46201 gcaatattga aacaaaaata atgccaacaa tcatcagctt aaggttgctc tgaatgaagg
    46261 ggagcgtgcc gaagaaataa cccgcgtagg taaaaatcag tacccacaac agtgcgccga
    46321 tcacgttata agcggcgaaa tgacggtacg acatgtggcc cattcccgca acaaacggcg
    46381 cgaacgttct gacgatcggt acaaaacggg cgagaataat cgttttgccg ccatgtctct
    46441 cgtaaaactg atgagtctta tcaagataac tgcgacgaaa gattttcgaa tccgggttac
    46501 tgaacagttt gtcaccaaat aaccgtccaa tggtatagtt tactgcatca ccgacaatag
    46561 cggcaatcaa cataagcacc accatcatat ggacattgag atcgttagtt tccagcgacg
    46621 ccaatgcacc agcaacaaac agcagcgaat caccaggcag gaatggcgtt accaccagcc
    46681 ccgtttcgca gaacagaatc aaaaacagaa tggcataaac ccagacgccg tactccgcga
    46741 ccagctctgc cagatgcacg tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt
    46801 ccatatttgc ctatgccttg tactcgtcat ctttcaggct gtaactacgt tggctacagc
    46861 ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa atagcgggcc cattggcggt
    46921 tttggaagat cataccggtc agggtaatcc accgcgacca gatacagccc ttccgctttt
    46981 gccgttgctg ccgccagcgt tctgtccttt gccgccagca actctgctat ccatctctcc
    47041 ggctggttgt gggcaccgac ttccatcagg ctgccgacaa tattcctgac catatgatgt
    47101 acaaaggcat tcgctttgat atctaccacc acataaggac cgtgacgcgt gacattaata
    47161 tgcataacgt tgcgccacgg ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa
    47221 tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg
    47281 taaaaatggg ttaccccttt actcaatacc gccgggcgca gccgatgatt gtagatgatg
    47341 tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa aatcatcagg tacagcttta
    47401 acccaacgca cggcgatgtc accaggtaaa ttcgcattta cgcccaaggt ccacgccgcg
    47461 tctttgcgct gcgcggttgt ttcgaaatgc accacctgcc cggtaccgtg tacccctgcg
    47521 tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg ccacctggga gagcgccttt
    47581 tccagcttct cctgcacact gcggacttca ttctgccgtt gccagccgta atacttactg
    47641 ccgtcgtact caatgcccag cgcaatttta taaactggca gttgttgctg gtcggacatt
    47701 agtacagata ctcctgcacc agtttctcgg cgatttttac tgccatcagc gcgccgccaa
    47761 agcgaacgtt atcggccacc gaccagaact ggacttgctc cggcataccg tagtcattac
    47821 gcacgcagcc aacagaaaga tgcggcgtac ccgaagcatc ccctacctga gtcgggaatt
    47881 cgttctcttc agagagcaca atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg
    47941 ccgccagcgg acgcagtgct tcaaagttga ccatctgggc atgaccgtag aataccggtg
    48001 cctggacgac gcttgccgaa atcatcagcc cttcgtcctg caggattttg cgtacttcgt
    48061 caacgatacg acgttcttca cgcacgctac cttcgctatc cggcaacaac ggcagcatat
    48121 tgaacgccag ttgacgaccg aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt
    48181 tcgcactctg ccccgctaac gcatcgaccg cttttttgcc ctgggcggag gctgaaatca
    48241 ggctggtaac actgatacgc gacaaaccgc cctgatcgat taacggtttc agtgccgcca
    48301 gcagctggct ggtcagactg tctggtacgg cgatgacatt ccggttgcgg taatcggtca
    48361 gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc gggttcgaga gcaaacagtc
    48421 cgctgctgtc gatcaccagg caacctgagt tggtcgcttc ttcaacccag gcagcggtag
    48481 cttctttgcc tgcgacaaaa aatgccagct gcgcctgcgt ccagtcgaat tcagcggcat
    48541 cctgcacagt gattgtttta ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg
    48601 ccagtgcata aatttcccca accgggaact gacgttcagc cagcgtttca agcagggctt
    48661 cgcccacagc gccagttgcg cccaggacgg caatgttcca gccttcagac atggtggttt
    48721 actccagaaa tagcaaagct ccctgccaat gttacagcag agagcatgaa gaagagatta
    48781 acgtgccgga tgatgaacgg cgttaaaacc cagtttacac agcaatgatg ccgcactggc
    48841 gtcatcacaa attacataca gagatgacca ttcacggcgc tcaagatagt ttttgcgcag
    48901 tttatcgaac tcacccggta tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac
    48961 atcatacacc aaatgcacca gccttttcag cgtcggttga tcgagcgggc catgcagcgt
    49021 aatgcgacca aactctggcg cgggcagtaa tgtatccagc gcaacgtgct gttcatgccc
    49081 aataaacttg ctataggctt caaacacttg cgtagtaccg cgtgctttac cttccagggt
    49141 ataacctgcg atatgcggcg tgccgatatc cacttttgtc agcaactcca cgttaagttc
    49201 cggttcgcct tcccagacat ccagcactac gcttaacttc tggccttcat tcaggcaggt
    49261 cagcaacgca gtattatcga cgactgcgcc acggcaggcg ttaatcagaa tcgctcccgg
    49321 cttcaggcta cggatcagtt tttcatccgc cagatgcagc gttttgtacg gcccatcttt
    49381 gaagagtggc gtatggaaag tcagaatatc cgcatgctga actaattcat ccagcgagcg
    49441 gaaatcaccc tcatccccac ggtcagcgcg aggcggatcg caaagcaatg ttttaatccc
    49501 taacgcttcc agtcgcgcct gcaatcgacg tccaacgtta ccaacgccca cgatccccac
    49561 cgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc atcagtagtg aggagaaaac
    49621 atattccacc accgcaatcg cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg
    49681 cttcaaccat gcttcatcga catggtcggt ccccgcagtg gcagtgccaa caaatttaat
    49741 gggttttcct gccagcaaag attcattcac tttcgtgacc gaacgcacca tcagcgcatc
    49801 cgcgtctgcc agttgagcga cggggattgg acgcccggga accgcggtca cctcacccaa
    49861 acggctaaat aagtcgcggg cataaggcat attttcatca acaaggattt tcacgtttgt
    49921 gttacctgta tgagacgaga gttaaccgga caagtgtgcc ataatctcgc ggccaggcat
    49981 acttgcgaag atttcaggta taaggatacg taatgataca acctatttcc ggccctcctc
    50041 ctgggcaacc accaggtcag ggagataacc tgccgtctgg cgcgggcaat cagcctttat
    50101 ccagtcagca acgtacttcg ctggaaagct taatgacgaa agtgacctca ctgacgcaac
    50161 aacaaagagc agaactgtgg gcgggtatca ggcacgatat tggtctgtcg ggagattcac
    50221 cgctgctttc gcgtcacttc cctgccgctg agcataatct ggcgcaacgt ctgctggccg
    50281 cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt aggggagtat ttacgtctgg
    50341 ggaataatcg tcaggcggtc acggattata tccgtcataa ctttggtcag acgccgctga
    50401 atcagctctc accggagcaa ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg
    50461 ttattccgca accacagcag cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca
    50521 atgcgctaaa acagctggtg accaaacttg cggcggcaac gggggaaccc agcaaacaga
    50581 tctggcaatc gatgctggaa ctttccgggg tgaacgatgg cgagttaatt ccagcgaaac
    50641 tgtttaacca tctggtgacc tggttgcagg cgcgccagac gttaagccag caaaatacgc
    50701 cgacgctgga atcactacag atggcgctaa aacaaccttt agatgccagt gaactggcag
    50761 cgttatcggc gtatatccag caaaaatatg gtctttctgc gcaatcatcg ctttcttctg
    50821 cccaggccga ggatattctt aatcagcttt atcaacggcg ggttaaaggg attgatccgc
    50881 gtgatatgca accgctgctt aatccttttc caccgatgat ggacacgttg caaaatatgg
    50941 caacgcgtcc cgcgctgtgg atactgttag tcgcgattat cctgatgctg gtctggctgg
    51001 ttcgttagcc ccgacgaaat gacagtatcg tgacaataat tcccagcacc gcagagatcg
    51061 ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc cagcattccc gccagcggcc
    51121 cggagacgcc gagggcgata tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa
    51181 cttgtgaggg gacgcgttta accacctcca cgcccagcgc aggaaagata agcgaacatc
    51241 cggctccggt taacgccgcg cccgctaatg cgacccacgc acctggggct tgccagagca
    51301 gcaacaagcc caccgtttct acaagcagag agacaatcgc cactttcacg ccgccaaaac
    51361 ggtccggcat ccagccaaac atgacgcgca tcacgacaaa tgcgccgcca aactcggtaa
    51421 gagtaaagcc cgccatcgcc catcctttgc tggcaaagta gagcgaaacg aaagtcccga
    51481 taaccgcaaa accaacgcct tgtagtgcca gccctaaccc tggtttccag ataaacccga
    51541 caacgctcca cagcgatgga cgttctcccg ccagggccgg tactttgcgc actgtgccgt
    51601 tacaggccca cgccagtaag ggtaatacca ttgtggtgat cgccagtgcg gcaaaaccgt
    51661 aatggctatg aatcaacagg ccaagcggag caccaacagc gagggcaccg taaatcgcca
    51721 ttccattcca tgacatcact ttgccagagt gttttggccc tacgatgcct aacccccagg
    51781 tcagagcgcc tgtcagtaac tggctttcac caaacccaag aatcaaacgc ccgatgacca
    51841 acagggcaaa tttgaacggt gcggagacag gcaaaatcgc cgccagcagc aacgcgccgc
    51901 cagccagacc acaagctaac atcccctgaa gcgccgaacg ttttgcgcca tattgatcgg
    51961 ccagtcgccc ggcatagcca cgcgtcagca ccgtagccag aaactgaatc ccgacggcaa
    52021 tgccgaccat ggtattgcca tagcccagtt catgatgaac aaacagcggg ataaccggca
    52081 acggcagccc tacggtcatg taggtgagaa aaaccgcaaa agcgatgcgg aagagcgaaa
    52141 aattggcaga agatcgtgtt tcggtttggc ttacagcagt catgcattac tccagaatgc
    52201 agcgcaaggc gaggagtatc cccgtctcat ctctctggtt tcagggttac ggtgcgttgg
    52261 caggatttaa cgcgtacgtc ttttcagaag gaaatcgaca aagcgggaag tttgcctgga
    52321 actggtggcg attgtcaatg atgtgaaaaa gggaaccatc aggttccctt ttgcgttagt
    52381 gccggaggcc gcattggcgc gtaacgtcgg atgcgacgct ggcgcgtctt atccgaccta
    52441 ctccggccta ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg
    52501 ccgaagccga agctgttaga cataacagtg gtcagttcgc gatcggtcgt ttcggtcacg
    52561 atgttcagac ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag
    52621 ccgtgttcca gcatcagcag agagtagata gcttcctgca cacccgcagc gcccagtgag
    52681 tgaccggtca tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg
    52741 atagctgcca gctctttcac gtcgccaacc ggagtcgaag taccgtgaga gttcaggtaa
    52801 tcgattgggg tatcaacgcc atgcatcgcc atctgcatac aacgtactgc gccttcgcca
    52861 gacggagcaa ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag
    52921 atgtgagcac cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg
    52981 ccagcgataa cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg
    53041 tcgttgtatt tagtagacag cgcacccatc gcgtcgaatt cgcaggccat ttcccagcac
    53101 agctcttcgc cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact
    53161 gcgttaccga tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca
    53221 tgaattttaa acggcgtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc
    53281 acatacgggc caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaatacc
    53341 tggaaacgcg gggaaccgcc gccagaacct gcaatcaggc caacgcgcgg gttattctgg
    53401 taagcttccg gagaaaggcc cgcatctgcg attgcctgct ccatagaaag gaatgcataa
    53461 atggatgcgt cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt
    53521 tttacgttgc cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg
    53581 atccctgaac gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg
    53641 gaaacaatgc ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac
    53701 atagagtaag tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc
    53761 catttaatag agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt
    53821 aagacgagta acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc
    53881 tgagggtaca cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct
    53941 ggaagagacg cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca
    54001 tccacatcct ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac
    54061 gctatggcag gcatttgatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt
    54121 acatttcatt agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca
    54181 acactggccg gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt
    54241 gcccggttgc catcgtttat tgctcaatga aggccgcgtg acgctggatt tatggtttgg
    54301 cgatattaac gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg
    54361 gtttctggac ggctttgcgc cagctaaaaa cccggatatg tggacgcaaa atctgtttaa
    54421 cgccatggca aggttggcgc gtccaggcgg cacgctggcg acatttacgt ctgccggttt
    54481 tgtccgccgc ggtttgcagg aagccggatt cacgatgcaa aaacgtaagg gctttgggcg
    54541 caaacgggaa atgctttgtg gggtgatgga acagacatta ccactcccct gctccacgcc
    54601 gtggtttaat cgtacgggca gcagcaaacg ggaagtagcg attatcggcg gtggtattgc
    54661 cagcgcgttg ttgtcgctgg cgttattacg gcgcggctgg caggtagcgc tttattgcgc
    54721 ggatgaagcc cccgcgctgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt
    54781 aagcaaacac gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg
    54841 gttttacgac caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg
    54901 ctgggatgag aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga
    54961 actggctgta gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg
    55021 cagcggcatt acttatccgc aaggtggttg gctgtgccca gcagaactga cccgtaatgt
    55081 gctggaactg gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt
    55141 atcccgtaag gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag
    55201 cgtagtggta ctggcgaacg ggcatcaaat cagccgtttc agccaaacgt cgactctccc
    55261 ggtgtattcg gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct
    55321 gaagcaggtg ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca
    55381 ttgtattggt gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca
    55441 gcagcagaat cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt
    55501 tgatgtcagt gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc
    55561 aatggtaggc aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga
    55621 gcagaaagat gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt
    55681 aggttctcgc ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag
    55741 cgacgaaccg attccgatgg atgccagtac gctgacggcg ttaaacccga atcggttatg
    55801 ggtgcggaaa ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt
    55861 tgccggatgc ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac
    55921 gcgataagcg tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct
    55981 gctgaaacag gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac
    56041 cggcaccaat ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt
    56101 ggtcatcacc actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac
    56161 tggcaaacaa ctcatcatca ctggcatggt caaccatgcc gtcgattaat gccagaatgc
    56221 gtgattcaaa ctccgcgatc atcttctttc ctcattgtaa aacgtggcac cggcttcagt
    56281 tgagcgcttc cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt
    56341 gtagtgcttt aatgaagcgt gccggacgct cgggaatgcc atttgtcaga taatccatca
    56401 cctgcgcatg tacccgccgt tgaaacacaa cacgatccgg ttcgaagtcg ccttccagat
    56461 tgtcgcaact gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgagtgcct
    56521 gcggcttcac ttcaacatct tcaaactggc tttgcgtttg ggcatcacgc ccatccgggc
    56581 aataccagta gccgaaatca accagctcac ggcgcgcttt tccggcaata caccagtgcg
    56641 aaatctcatg aattgcgctg gcataaaagc cgtgggcaaa gacaattcgg ttatacggta
    56701 cttccgcatc agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa
    56761 attcatcggc aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt
    56821 tcattagttc atccccaacc agtggaggat ctcctgtccg tggctatcgt aaagtaattt
    56881 ggcactcatc accgccgaga cgataacaat catcgggcgg atcagctttt gtcctttgct
    56941 caacaccagc cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc
    57001 cgtcgcccaa atcactttgc cgccgagaat aaacagcagc agaccgccaa tgtttgacgt
    57061 tgcgttgagt aatttggcgt gagccgtggc tttggcgagg ttgaatccgc acagcgtaac
    57121 gaaggccagc gcgtaaaacg atccggcagc cgggccaaag aatccgtcat aaaaaccgac
    57181 gcagccaccg gcaattaacg cgaagggtaa accgtacatc cggcgctggc gatcttcttc
    57241 acccagcttt ggcatcagca agaaatagag gccgatacaa atcaccagaa tgggcaaaat
    57301 ctgccgtaag acatcagcct gaacgtactg taccaacagt gcgccgctca ttgagccgac
    57361 aaaggtcatg gcaatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa
    57421 gtagatagtg gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcgtt
    57481 agcgggagac attcccgcag ccatcaatgc cggaatggtg agtaacccac cgccaccggc
    57541 aatcgagtcg ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg
    57601 ggaaaccata aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc
    57661 tggcaggaag gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt
    57721 agaggtgcaa accagctttg cagttctgcc ccgcaaccat cgcctgatgg cggtaaaggt
    57781 tgatcttcac actccagact atcggcaggg caacgtaatc gtacatgcat atgcgcgcga
    57841 tgctggaacc agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa
    57901 agttgttgtt taatcgccgg attaacaaaa atgcgcgtga cgtctttgtc ctgggcagcg
    57961 agtttgatca agctgaaaat ttctggcttc cacagcgtgg agacaacgtg tttaccgtcg
    58021 cgggaaacta agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc
    58081 ggcagttgca gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg
    58141 aaacgcccac cagcgggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg
    58201 ctcacctggc tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga
    58261 cgctgatcgg tacgcatgac ctgataatgt tcggactgta tcggcaacgt gtcagcgccg
    58321 acaatacagc cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt
    58381 attttttgcc acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca
    58441 atcgcggttt tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc
    58501 gcccgctgcc gtaacaggtg atccattaaa acgatcgcca gcatcgcttc agcgatcggc
    58561 actgcacgga tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg
    58621 ccaaagcggt taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata
    58681 tgagcaatga tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc
    58741 tggaaaccgt ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca
    58801 aaaccgtcgc caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg
    58861 tcggcatcca ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca
    58921 acggtgactt tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct
    58981 aacgcgtcga ttttgtccgg gtccgggcag aagaacggat tttgttcaac cagcgaccag
    59041 tctttgattt ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca
    59101 aatttctcgg cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg
    59161 cgggcggaag aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg
    59221 taatcagcat ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg
    59281 tcagtgtttt cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg
    59341 gagagaattt tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg
    59401 cgacgacggt cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca
    59461 ccatcgacga tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag
    59521 agttgtccaa ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac
    59581 ttaatcttta taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat
    59641 aaacacacca tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg
    59701 ttccataaga tgtaccatgc tgttgccgac ttcacaaatc aacacgccat catcagcaag
    59761 gtaatctgcc gcgtttccaa gaatgcgacg cgtcagttta aggccgtcgg tgccagatgc
    59821 caggcccagt tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc
    59881 gacatacggc gggttagtga caatcagatc gtactgcact ttcggcaaat cacggaacag
    59941 atcggaacga atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc
    60001 aaccgccagc gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata
    60061 agcacaggca atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg
    60121 cttgctaatg agtccggcaa atttattgtt gatcagttca ccaatcggcg agcgcggcac
    60181 cagcacgcgt tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta
    60241 agccaccgga atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc
    60301 gctggaggtc agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga
    60361 tggtaacacc agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat
    60421 atttgccgcg ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc
    60481 atttactgct tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata
    60541 atttcggcgg ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg
    60601 aggaaaaata cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt
    60661 agaaatagag acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg
    60721 tttcgccagt tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg
    60781 cagcgtaaaa aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc
    60841 agccattatt tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat
    60901 gttcgcccgg atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg
    60961 gaattgtttt tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg
    61021 ttgattgccg cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg
    61081 aagcatattt tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca
    61141 tttcaccagg caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg
    61201 gaagagtggt tgccgccgga gttgccctga atgctggcag caaatgccgg agcagcgtaa
    61261 acgccttatc cggcctacaa atatgtgata attcaataaa ttgcaattca cttcgtaggc
    61321 ctgataagac gcatcagagc aatactacga actacagttc attcaataca tactgaaaac
    61381 gacacgtcga atcaatcggt tgcccactac tggtattcac attcaccaca atatcgtgta
    61441 gtactttatc cgttaccgga tgacgagttg gcgtaaccgt taccgttgat ttatcacaac
    61501 cgtaagaggt aaaggttaac aattctgata ccgcgcggca gtatgctcct ctgccatcat
    61561 tatcagaaaa attactgcca tcacagagtc tgccgccttc catcaggtag gtcgaaagcg
    61621 tggtggtcac tgttgacccc gtggactgaa ttcgtaacgc cttattatgg ctgacataat
    61681 tttcactcca ggagatatcg tatgaagtga ccggtatcgt tgggcatatt gtgttatgca
    61741 ctgtaggggc cgtatagctt ccaaatccgg gtaaatctgt ataggaataa aaggatgcga
    61801 tccaagggca accataatca tcagatgcaa cggtaatttt aaacgtcata tatgcacaac
    61861 gaaaaatttt cgtatctatg ccagggcaat ttgagttatt aattgtatca aagtcagtca
    61921 gtaccaaagt tggagctgaa cccgacccag accaggaaat aacccgcaac gtatcgcctt
    61981 tatacgcaaa agaatcatat ttcccatatg taacctcctt tggcgttatc accgtaacat
    62041 tgaaaacact gctcgctaca gcaccggtta ccttgtccat ggcataataa actctcacac
    62101 tgtcaaaact cgcactcata agtttatctc cagcataact cttagatgcg ccgacaacac
    62161 tcagaacgag cagcattagg ctcgcgaaaa ttatggtcca ttttttcatt tcagaaatac
    62221 tcaaaggttg ccgtcactgt ggcactaaag tctccggacg ttacatctcg cccgctcttt
    62281 gcctgtaacc aggtattaaa atttaggctg ttatttccct gattcagcgg gagcgtgaca
    62341 cctgttgtat tgttgattgg caccgatgtg cctgccgcag tttcaatacc aatcccagcg
    62401 ccggatgctg atgacgagga gtcaaaggcc agaaagccgg gtagagcact atcttctgtt
    62461 cctgtcaacg ttactttgac attaaacaat gtagaactat gacaatcttt caattgaaac
    62521 accaatggca ctcgctctga ctgcccaagg ttcattaaat cgctggcggc aatggcagga
    62581 aaatgtactt ctgccaactg gctaccatta atcactggcg tacacgattt actgattaaa
    62641 ttgccggtaa agtgcaaatt atctcctgct attgtggttc cactgcccag caacatcagg
    62701 ctactaataa aaaacaacgt acgctttttc ttcattggta atctacctgt aacgtggccc
    62761 aggcctcaaa gtccgcctct ggcagaacga caccactctt ttttactggc acagccgtta
    62821 acaccggtaa atcggtttca ttcaccataa gtggcgtatt gatcgtaaaa ctctgccctg
    62881 cctgtttgag ctgaatgcct aaaccagtga tattgctgct tactgcagca ttatcaaaat
    62941 cgcttagtgt gccactccag ctcaaggtca tttccattaa tgggtcgcga accgccgtat
    63001 cgcaggtaat ggaatacggc acggtttgct cataattgct gccattaatg tcatcaataa
    63061 gcacatcctg aaaagttatt tcaatggttt gagcgttgct aatcgtgcag ttaggcggct
    63121 gcactaaata accgtgaaaa gagacgtttg agagtgccat cgagcaaaag ccccacagca
    63181 aaataagcga gatccgtttc attggtaatc caccacaatt gtggcactgg cgttaaattc
    63241 agctgccgct aacgttgtac cactctgttt caccggcaca gcttccagaa cgggaactcc
    63301 cgaggaaaaa ttaaatggca accatgagcc agaggttaag tcgagtattg tgtgatcact
    63361 acttttctga acccgaatac caaacccagg aatacccgtg ctgataactg tttcaccgtt
    63421 aatcacgacc gttgtcgcct gcatctgtag acgcaggtca tccattgcca gattgttaca
    63481 aacaagcgag tagtcaatgg gacgtttata atcaacccca ttgatcttat taatgaatac
    63541 attaccaaac tcaactgccg caccatttac cgtacaagga ggaggggcat cgacaagcac
    63601 ccgtaaattc atacttactg acttactcgc tgcaactgca aagccgctac caacacaaca
    63661 tatcagtaac ccagttaccg cccagacaga acaagatcct ttcatactgg ctctcccggc
    63721 ctcagccttt gcggctctgc tcttcatcga cctggcaact gttaccgttg caagtgaaaa
    63781 agagcggcat ccgagcgcca tagtcgttga cataggtcag taccggcacg ctacccatct
    63841 tcacattcag cggtgcagag gttttcggcg acatcactat tggcgagaaa ccggctgcag
    63901 gattgccatt tttctgtgtg ctggcattac tgaaaatgac gtaatacggc gtcgggttat
    63961 tgacggtaaa ttcctgaccg ttgcgggtta aagtaacctt atattgccag gggtttttca
    64021 tgctgacgtt ctccagcgcc ttcggtcgcc agaacaactt aatacgcgtt tgtagtgcta
    64081 tttgcagcgt gttggctttg tttgattttg gcgggatctc acgcacgttg aagtagaaca
    64141 ggctttcacg atcggcaggc aatttgttga tatccggcat tccctgcact tttacctgcc
    64201 cgttcatcat tgaatcgatg cgttgcaccg gtggcagaac agttaacggt gaactgatct
    64261 tgttaccctt ttcatcttcg atccagcttt gggccagata aggcagtttc ggatcgttat
    64321 tgcgcaacgt cacgctgatt gatttgtcgc tttcattaaa tatcaaacgg gtacgatctg
    64381 gcgttacaga agcctgggcg acaaactagc agcgctaagc agtaacgcta gcgtcgtagc
    64441 acccaatttg gcagagcaaa gtaaatcaga catggatcat tccttaatca cgttattggt
    64501 tagctgaaac agaaacaggg ggattggtgg gtgtcacctg ttgcgtctgc tcctgaatga
    64561 caggctttat gtcatcagga acaaccggcg cgacgttgcc tttatgctgg catggcagta
    64621 acaagccgtt aaacagatcg gcaggcagcg ggtccggcag gttgatatcg caatgcgcaa
    64681 caccactcca gaacacactc atgtgttcgc caggtttcac cccagccaga taaacattgc
    64741 cgtcatcatc gacaaggccc actgtctgct cgttatcatt ttttacttct gcgccaaacg
    64801 gaggatggct gccgtcttgt aaacgcagca ccgccatcgc tttttgacca ctgatgacgg
    64861 caaatttgcg gtagccaatc gccccttcag ttagcgtggc ttgtaccacc gactgggttg
    64921 cttcggcgtt ttccggcagt ttgttgaggt cgatatacgc ctgattgcgg taatagttat
    64981 tgacgtcaga aacgacggct ttaccaaaca tattggtata aacagcagca ccgttacctt
    65041 ctaccggaac atcggcaacg ccatcggcat caatcaacaa gcgtgtcccg cccatattct
    65101 gggtacggtg aagtgcgcca ccgtgggcag taagcgttgc gccgccctgt aacgacaagc
    65161 ccgcagaggt gtattgccct tcatggtagt tcgcactgag gtcaacctgc gccagcgaac
    65221 catcatggct gtaatagccg tcaacgctgg tgtgtttgtc actggtgccg acgttcaact
    65281 gatagtgagt cgcgtcatcg acacggctga aataaccgac ctgactgctg tccgtcccac
    65341 tgccatagtt accgttatag ctaacggtac tgttgtcacc ccacggcata ctgagcgaaa
    65401 tgtacatgcc tttgtcggcc tggttgtcat actcgtaacg gtagccagtc atcgagatgc
    65461 tgacattacg aatactgccc atattgaaat agtgcgagag catgatgttg tagtttgtct
    65521 gctcctcgcg atcccagtag gtatggcggg tatagttgag ataaaccgaa acacccgcat
    65581 cgcggaagtt ctggttataa gtggcagtgt acatctcttt gtcgttgccc gtgcgtacca
    65641 ttccgctgtc actggcatcc agatactcgc tcatggtcat aaagttctct tccgagaagc
    65701 gatatccagc aaaagtaacg cggctgttga gctggtcaaa gtctttggaa taactcacac
    65761 ggaaggagtt accgtccagc gaacctttgc cataagcggt gtctttatcc agtttggtgt
    65821 gcgagtgagt aacatcaaac gcgaccgcgc cgaatgtaga caaatcgcga ccgacgccaa
    65881 gcgccgcaga ctgatagttt tcatctccca gtgcgccgcc atataacgac cagccgttag
    65941 cgatccccca ggaagcttca gcaccagaaa aaaattcacc ctcgacatgg tgtccccact
    66001 cttgcggacg gcccatcatg atcttatagc gaacctgacc tggacgagtg aggtatggca
    66061 tcaaggcggt gctgatgtca tattcctgca cctggccgtt ctgttcttca atgcgaatat
    66121 gcaacgtacc ggagacagaa tcaccaagat cctgaatacg aaacggtcca gccggaacct
    66181 gcgtttcgta aatcacacgc cccatctggc tgacggtcac ttttgctgtg gtgtgtgcca
    66241 cgccggaaat gtctggcgcg tagccgcgca gattgggagg caacatttga tcgtcagtac
    66301 tgacactgcc accaacatag ttaaaaccat caaaaatatc ggatctgagg taatcctcgc
    66361 ccagcgccag tttggctttt aatgatggta acgcccgcta ggcatagtag cgactccact
    66421 cccatttttt ttgagtttca tcgccgctga attcatcgtc atcattactg cgagtatgtt
    66481 gatagttagt ctgccagtca gcacgcatac gccacggccc caggttaacc ccgaccgtcc
    66541 cgttgccgct gatctcgtta ctatcatcac cgccattttc ttcgtgccgg gtttgtgcgt
    66601 tgatgctgta gtccgcgacg atcccggaga tgccgtcatc ccaacgtgaa ggcggatccc
    66661 agtcgggata agtatattcg aggtaagcct gtggcagtga aatgactaat gcggactggc
    66721 ttaaatcagc cttaatttcc atgccttcca gttgaccgga tttcaggcat ttagcatcgt
    66781 ggctccattg cagatttttc gccacgtctt cttttaaacc aaactgcgct accagttccg
    66841 gtgtcagaca agcatagctt ttgctcgcgt catcttcacc agcataccag taaatatcgt
    66901 actcttccgc caatggctgt ttatttagtt gaacctgtaa attgtatttt ccgggctcaa
    66961 catatccctg gctggaaaaa cgcttcagat caatttttgt gtcgcctttt aattccagaa
    67021 aacgggaatc aaactgaatg tcgtcttcag cccagacact gctataactc cctgacattg
    67081 ccagcgcaat gcaccacgga agaatccgta atcgaaaaag agaatggtca ggcatacacg
    67141 tttctatccg tttttattct tgttcggtca aaatccattt aaatccccca tagcagtgtc
    67201 gctatggggg tatgccttac aggtaagtaa tctggaaggt ggtatttgct tcgaaattac
    67261 ccagatctgg cgcatcagct gcgccgacca gccaggcttt aaagttcagt gtctgcttcg
    67321 ctttgccttt attggtagaa gtgtctttca ctattttctg gtcaataccc atgccgcttt
    67381 tgtaagaggt acccagagag tcaccgatcg ccaggctaac attgttaaat gccgcacagg
    67441 tatcggtatt gaaaatggtg taataattgc cgctatttgc agaagaaacg gtaccagtaa
    67501 aggtagtggt cattgtttcc tgagtatcaa atacgcaatc ttgcagacga atctggaaac
    67561 ttttcggcgt ggtggtgccg ttatttttca gtttttcggc accgatatca cccatatcaa
    67621 cttccagttt gtgatcgtcc ggaacgatag aacacactga gtcttcaatg gtgccgtaaa
    67681 aacgtgcagt accgttgtta cccgcagcga ttgttgccgt agaggtgaac acgcccatca
    67741 ccattgccgc agcaatagct gttttaacaa acttactcat tatttgtcct taaactataa
    67801 ataattaaaa atataaacat tgcaatacat tgattcagtc aataaccaat gtttataccc
    67861 agccattgaa tacggcatgc gatccaaaaa gaacaggtcg tagaaaattg tattcgttgg
    67921 tg
//
LOCUS       DAZPBM010000003        60957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPBM010000003 DAZPBM010000000
VERSION     DAZPBM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.003"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHUZQ_000142"
     CDS             <1..468
                     /locus_tag="ACHUZQ_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1023954.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHUZQ_000143"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHUZQ_000143"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1023955.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHUZQ_000144"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHUZQ_000144"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1023956.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUZQ_000145"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUZQ_000145"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1023957.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHUZQ_000146"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHUZQ_000146"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1023958.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHUZQ_000147"
     CDS             complement(4084..5313)
                     /locus_tag="ACHUZQ_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1023959.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHUZQ_000148"
     CDS             complement(5377..5817)
                     /locus_tag="ACHUZQ_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1023960.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHUZQ_000149"
     CDS             complement(5922..6692)
                     /locus_tag="ACHUZQ_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1023961.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHUZQ_000150"
     CDS             complement(6689..7615)
                     /locus_tag="ACHUZQ_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1023962.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHUZQ_000151"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHUZQ_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1023963.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUZQ_000152"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUZQ_000152"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1023964.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUZQ_000153"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUZQ_000153"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1023965.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUZQ_000154"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUZQ_000154"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1023966.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHUZQ_000155"
     CDS             13322..13669
                     /locus_tag="ACHUZQ_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1023967.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHUZQ_000156"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHUZQ_000156"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1023968.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHUZQ_000157"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHUZQ_000157"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1023969.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUZQ_000158"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUZQ_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1023970.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUZQ_000159"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUZQ_000159"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1023971.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHUZQ_000160"
     CDS             18978..20735
                     /locus_tag="ACHUZQ_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR1023972.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUZQ_000161"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHUZQ_000161"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1023973.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUZQ_000162"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHUZQ_000162"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1023974.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUZQ_000163"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHUZQ_000163"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1023975.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUZQ_000164"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHUZQ_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1023976.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="ACHUZQ_000165"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="ACHUZQ_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1023977.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHUZQ_000166"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHUZQ_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1023978.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHUZQ_000167"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHUZQ_000167"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1023979.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="ACHUZQ_000168"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="ACHUZQ_000168"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1023980.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHUZQ_000169"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHUZQ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1023981.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34428
                     /gene="gspE"
                     /locus_tag="ACHUZQ_000170"
     CDS             33043..34428
                     /gene="gspE"
                     /locus_tag="ACHUZQ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR1023982.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34418..35620
                     /gene="hofC"
                     /locus_tag="ACHUZQ_000171"
     CDS             34418..35620
                     /gene="hofC"
                     /locus_tag="ACHUZQ_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1023983.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHUZQ_000172"
     CDS             complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHUZQ_000172"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1023984.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36854..36898)
                     /locus_tag="ACHUZQ_000173"
     CDS             complement(36854..36898)
                     /locus_tag="ACHUZQ_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1023985.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36923..37543
                     /gene="coaE"
                     /locus_tag="ACHUZQ_000174"
     CDS             36923..37543
                     /gene="coaE"
                     /locus_tag="ACHUZQ_000174"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1023986.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37543..38286
                     /gene="zapD"
                     /locus_tag="ACHUZQ_000175"
     CDS             37543..38286
                     /gene="zapD"
                     /locus_tag="ACHUZQ_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1023987.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38296..38493
                     /gene="yacG"
                     /locus_tag="ACHUZQ_000176"
     CDS             38296..38493
                     /gene="yacG"
                     /locus_tag="ACHUZQ_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1023988.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHUZQ_000177"
     CDS             complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHUZQ_000177"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1023989.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHUZQ_000178"
     CDS             complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHUZQ_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1023990.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHUZQ_000179"
     CDS             complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHUZQ_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1023991.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHUZQ_000180"
     CDS             complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHUZQ_000180"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1023992.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHUZQ_000181"
     CDS             complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHUZQ_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1023993.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHUZQ_000182"
     CDS             complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHUZQ_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1023994.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHUZQ_000183"
     CDS             complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHUZQ_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1023995.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHUZQ_000184"
     CDS             complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHUZQ_000184"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1023996.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHUZQ_000185"
     CDS             complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHUZQ_000185"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1023997.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHUZQ_000186"
     CDS             complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHUZQ_000186"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1023998.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHUZQ_000187"
     CDS             complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHUZQ_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1023999.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHUZQ_000188"
     CDS             complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHUZQ_000188"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1024000.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHUZQ_000189"
     CDS             complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHUZQ_000189"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1024001.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHUZQ_000190"
     CDS             complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHUZQ_000190"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1024002.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHUZQ_000191"
     CDS             complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHUZQ_000191"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1024003.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHUZQ_000192"
     CDS             complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHUZQ_000192"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1024004.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHUZQ_000193"
     CDS             complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHUZQ_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1024005.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHUZQ_000194"
     CDS             complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHUZQ_000194"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1024006.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHUZQ_000195"
     CDS             complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHUZQ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1024007.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16851 c        15001 g        14509 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacta gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaagc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat taaagccgcg
    33961 caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta
    34021 cgtttacagc aaatgggagt cgcccgctgg atgctctcat cagcgcttac gctggtaata
    34081 gcccagcgtc tggtacgtaa actttgccca cattgtcgcc agcagcaagg ggagcccatc
    34141 catattccag tcaatgtatg gccgtcgccg ctgccccact ggcaggcacc cggttgtgta
    34201 cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg
    34261 gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga
    34321 caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc
    34381 acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg
    34441 ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg
    34501 tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat
    34561 cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc
    34621 gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca
    34681 tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat
    34741 tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat
    34801 gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca
    34861 gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat
    34921 cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt
    34981 tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac
    35041 gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc
    35101 gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct
    35161 gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac
    35221 gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag
    35281 agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag
    35341 taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt
    35401 gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc
    35461 ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc
    35521 gacgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc
    35581 aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca
    35641 ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata
    35701 aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa
    35761 cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta
    35821 acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca
    35881 gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga
    35941 ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg
    36001 ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg
    36061 cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg
    36121 cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca
    36181 gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg
    36241 gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa
    36301 ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta
    36361 gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca
    36421 gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca
    36481 gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata
    36541 tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga
    36601 cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg
    36661 tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg
    36721 gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc
    36781 aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg
    36841 agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga
    36901 tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag
    36961 tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga
    37021 tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca
    37081 ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg
    37141 gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca
    37201 gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc
    37261 attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag
    37321 cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga
    37381 acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat
    37441 tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta
    37501 tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt
    37561 tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag
    37621 caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat
    37681 gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa
    37741 cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc
    37801 cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg
    37861 cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc
    37921 atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc
    37981 caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag
    38041 gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagt
    38101 ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg
    38161 ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt
    38221 atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt
    38281 tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg
    38341 gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc
    38401 gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa
    38461 agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct
    38521 gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac
    38581 gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa
    38641 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    38701 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    38761 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    38821 aagttcacgc actaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa
    38881 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    38941 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    39001 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    39061 cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc
    39121 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    39181 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    39241 tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac
    39301 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    39361 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgccgc taggtgctct
    39421 ttccacagag agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca
    39481 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca
    39541 cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg
    39601 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt
    39661 gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg
    39721 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    39781 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg
    39841 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    39901 tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata
    39961 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    40021 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg
    40081 atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    40141 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    40201 ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga
    40261 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg
    40321 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    40381 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    40441 tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga
    40501 cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg
    40561 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    40621 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    40681 gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    40741 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga
    40801 gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac
    40861 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    40921 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    40981 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    41041 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    41101 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    41161 tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    41221 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    41281 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    41341 gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa
    41401 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    41461 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    41521 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    41581 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    41641 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    41701 cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa
    41761 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg
    41821 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    41881 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    41941 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    42001 ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt
    42061 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt
    42121 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    42181 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    42241 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    42301 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    42361 acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa
    42421 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    42481 atgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    42541 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    42601 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    42661 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    42721 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    42781 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    42841 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    42901 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    42961 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    43021 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    43081 acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    43141 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    43201 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    43261 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    43321 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    43381 gggcgtaacg tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    43441 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    43501 gaagtgtact atacatccgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    43561 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    43621 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    43681 cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    43741 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    43801 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    43861 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    43921 gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag
    43981 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    44041 agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac
    44101 gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac
    44161 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    44221 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    44281 ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac
    44341 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    44401 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa
    44461 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    44521 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    44581 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg
    44641 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    44701 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    44761 tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    44821 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    44881 ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc
    44941 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    45001 agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc
    45061 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    45121 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    45181 cagactccgt ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    45241 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    45301 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    45361 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    45421 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc
    45481 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    45541 cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    45601 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    45661 cagcacacga tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    45721 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    45781 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    45841 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    45901 tttatccata ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    45961 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    46021 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    46081 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    46141 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    46201 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    46261 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    46321 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    46381 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    46441 gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca
    46501 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    46561 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    46621 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    46681 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    46741 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    46801 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca
    46861 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    46921 acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    46981 ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc
    47041 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    47101 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    47161 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    47221 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    47281 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    47341 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    47401 taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc
    47461 gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    47521 agatgccatc actccgctgc cggtataagg caagcccatc agctcaagca tgccctgcag
    47581 cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga
    47641 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc
    47701 acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt
    47761 cccgcccaac aggaccgcaa ttttatcagt catgttgttc ttcctccgga gtttgcggct
    47821 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    47881 gaatcaggtc gttaccgatt aataccggtg ccagcatctc ggctacctgc gcagggtccg
    47941 gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    48001 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    48061 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    48121 gctgaaacag cattactagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg
    48181 cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    48241 cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga
    48301 agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa
    48361 ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg
    48421 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    48481 ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg
    48541 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    48601 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    48661 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    48721 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    48781 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    48841 agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc
    48901 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    48961 cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca
    49021 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    49081 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    49141 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    49201 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    49261 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    49321 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    49381 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    49441 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    49501 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    49561 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    49621 cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg
    49681 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca
    49741 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    49801 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    49861 ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc
    49921 attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    49981 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    50041 cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt
    50101 gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc
    50161 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    50221 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    50281 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    50341 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    50401 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    50461 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    50521 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    50581 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc
    50641 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    50701 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    50761 agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc
    50821 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    50881 gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc
    50941 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    51001 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    51061 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    51121 cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca
    51181 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc
    51241 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    51301 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga
    51361 tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    51421 attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt
    51481 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    51541 ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    51601 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    51661 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca
    51721 tatcgcccag ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt
    51781 gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca
    51841 aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat
    51901 cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg
    51961 tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca
    52021 ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc
    52081 gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct
    52141 gcccggaaag tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg
    52201 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    52261 gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt
    52321 tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc
    52381 ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag
    52441 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca
    52501 gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    52561 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    52621 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    52681 cagcggcagc gcttaaggat ggatgcgcct gtgcaatacc gggactggcg acaatcagat
    52741 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta
    52801 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    52861 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt
    52921 taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag
    52981 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    53041 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    53101 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    53161 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    53221 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    53281 cccgactatc gccgtgcaga caatgaccag ttcccctgcg tgtcgcagat acggtatatg
    53341 caagtagctg gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc
    53401 gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt
    53461 gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat
    53521 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta
    53581 cagggcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    53641 caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc
    53701 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    53761 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    53821 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    53881 caaatgagct atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    53941 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    54001 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    54061 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    54121 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    54181 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    54241 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    54301 cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt
    54361 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    54421 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcattaagc
    54481 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    54541 cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta
    54601 ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag
    54661 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    54721 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    54781 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    54841 gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca
    54901 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg
    54961 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    55021 gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc
    55081 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    55141 attaactgcg gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca
    55201 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    55261 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca
    55321 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    55381 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    55441 caatctggta atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct
    55501 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    55561 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat
    55621 cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    55681 tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca
    55741 ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    55801 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    55861 cggcggtttt cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga
    55921 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc
    55981 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta
    56041 accagcgtcc gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg
    56101 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    56161 aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat
    56221 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    56281 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct
    56341 ccaccagccc cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg
    56401 ggatcacttt cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa
    56461 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    56521 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    56581 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    56641 cgtcggtcgc ctcatctttc gctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    56701 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    56761 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    56821 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    56881 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    56941 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    57001 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    57061 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    57121 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    57181 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggatccc
    57241 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    57301 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    57361 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    57421 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    57481 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    57541 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    57601 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    57661 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    57721 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    57781 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    57841 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    57901 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    57961 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    58021 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    58081 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    58141 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    58201 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    58261 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    58321 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    58381 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    58441 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    58501 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    58561 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    58621 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    58681 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    58741 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    58801 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    58861 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    58921 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    58981 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    59041 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    59101 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    59161 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    59221 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    59281 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    59341 gatgtttaaa cttatctttc accggcgttg caacagccac gacttccgcc agttctttgg
    59401 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    59461 aacgctcttc accataggtt ttcaataccc aagcgatatc ggcttcttct gcggtttgta
    59521 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    59581 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    59641 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    59701 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    59761 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    59821 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    59881 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    59941 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    60001 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    60061 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    60121 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    60181 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    60241 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    60301 tgaccgacag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    60361 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    60421 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    60481 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    60541 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    60601 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    60661 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    60721 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    60781 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    60841 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    60901 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAZPBM010000004        59077 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPBM010000004 DAZPBM010000000
VERSION     DAZPBM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.004"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(109..1056)
                     /gene="dusC"
                     /locus_tag="ACHUZQ_000196"
     CDS             complement(109..1056)
                     /gene="dusC"
                     /locus_tag="ACHUZQ_000196"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1024008.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1295..1693
                     /locus_tag="ACHUZQ_000197"
     CDS             1295..1693
                     /locus_tag="ACHUZQ_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1024009.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1690..2385
                     /locus_tag="ACHUZQ_000198"
     CDS             1690..2385
                     /locus_tag="ACHUZQ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1024010.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2515..3399
                     /gene="cdd"
                     /locus_tag="ACHUZQ_000199"
     CDS             2515..3399
                     /gene="cdd"
                     /locus_tag="ACHUZQ_000199"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1024011.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3549..4268
                     /gene="sanA"
                     /locus_tag="ACHUZQ_000200"
     CDS             3549..4268
                     /gene="sanA"
                     /locus_tag="ACHUZQ_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1024012.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4271..4510
                     /locus_tag="ACHUZQ_000201"
     CDS             4271..4510
                     /locus_tag="ACHUZQ_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1024013.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4704..5942
                     /gene="preT"
                     /locus_tag="ACHUZQ_000202"
                     /pseudo
     CDS             4704..5942
                     /gene="preT"
                     /locus_tag="ACHUZQ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            5936..7171
                     /gene="preA"
                     /locus_tag="ACHUZQ_000203"
     CDS             5936..7171
                     /gene="preA"
                     /locus_tag="ACHUZQ_000203"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR1024014.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7298..8308)
                     /gene="mglC"
                     /locus_tag="ACHUZQ_000204"
     CDS             complement(7298..8308)
                     /gene="mglC"
                     /locus_tag="ACHUZQ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1024015.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8324..9844)
                     /gene="mglA"
                     /locus_tag="ACHUZQ_000205"
     CDS             complement(8324..9844)
                     /gene="mglA"
                     /locus_tag="ACHUZQ_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1024016.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(9905..10903)
                     /gene="mglB"
                     /locus_tag="ACHUZQ_000206"
     CDS             complement(9905..10903)
                     /gene="mglB"
                     /locus_tag="ACHUZQ_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1024017.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11183..12181)
                     /gene="galS"
                     /locus_tag="ACHUZQ_000207"
     CDS             complement(11183..12181)
                     /gene="galS"
                     /locus_tag="ACHUZQ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1024018.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(12365..13522)
                     /gene="yeiB"
                     /locus_tag="ACHUZQ_000208"
     CDS             complement(12365..13522)
                     /gene="yeiB"
                     /locus_tag="ACHUZQ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1024019.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13539..14207)
                     /gene="folE"
                     /locus_tag="ACHUZQ_000209"
     CDS             complement(13539..14207)
                     /gene="folE"
                     /locus_tag="ACHUZQ_000209"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1024020.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14465..15301
                     /gene="yeiG"
                     /locus_tag="ACHUZQ_000210"
     CDS             14465..15301
                     /gene="yeiG"
                     /locus_tag="ACHUZQ_000210"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1024021.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15333..17324)
                     /gene="cirA"
                     /locus_tag="ACHUZQ_000211"
     CDS             complement(15333..17324)
                     /gene="cirA"
                     /locus_tag="ACHUZQ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR1024022.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(17616..19085)
                     /gene="lysP"
                     /locus_tag="ACHUZQ_000212"
     CDS             complement(17616..19085)
                     /gene="lysP"
                     /locus_tag="ACHUZQ_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1024023.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19290..20171)
                     /gene="yieE"
                     /locus_tag="ACHUZQ_000213"
     CDS             complement(19290..20171)
                     /gene="yieE"
                     /locus_tag="ACHUZQ_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1024024.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20270..21319
                     /locus_tag="ACHUZQ_000214"
     CDS             20270..21319
                     /locus_tag="ACHUZQ_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1024025.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21393..22250
                     /gene="nfo"
                     /locus_tag="ACHUZQ_000215"
     CDS             21393..22250
                     /gene="nfo"
                     /locus_tag="ACHUZQ_000215"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1024026.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22254..23342
                     /locus_tag="ACHUZQ_000216"
     CDS             22254..23342
                     /locus_tag="ACHUZQ_000216"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1024027.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23398..24648)
                     /locus_tag="ACHUZQ_000217"
     CDS             complement(23398..24648)
                     /locus_tag="ACHUZQ_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1024028.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24748..25689)
                     /gene="rihB"
                     /locus_tag="ACHUZQ_000218"
     CDS             complement(24748..25689)
                     /gene="rihB"
                     /locus_tag="ACHUZQ_000218"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1024029.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25819..26517
                     /locus_tag="ACHUZQ_000219"
     CDS             25819..26517
                     /locus_tag="ACHUZQ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR1024030.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26584..27834)
                     /locus_tag="ACHUZQ_000220"
     CDS             complement(26584..27834)
                     /locus_tag="ACHUZQ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1024031.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27928..28866)
                     /gene="psuG"
                     /locus_tag="ACHUZQ_000221"
     CDS             complement(27928..28866)
                     /gene="psuG"
                     /locus_tag="ACHUZQ_000221"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1024032.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28854..29795)
                     /locus_tag="ACHUZQ_000222"
     CDS             complement(28854..29795)
                     /locus_tag="ACHUZQ_000222"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1024033.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(30253..31944)
                     /gene="fruA"
                     /locus_tag="ACHUZQ_000223"
     CDS             complement(30253..31944)
                     /gene="fruA"
                     /locus_tag="ACHUZQ_000223"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1024034.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(31961..32899)
                     /gene="fruK"
                     /locus_tag="ACHUZQ_000224"
     CDS             complement(31961..32899)
                     /gene="fruK"
                     /locus_tag="ACHUZQ_000224"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1024035.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32899..34029)
                     /gene="fruB"
                     /locus_tag="ACHUZQ_000225"
     CDS             complement(32899..34029)
                     /gene="fruB"
                     /locus_tag="ACHUZQ_000225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1024036.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34397..35578
                     /gene="setB"
                     /locus_tag="ACHUZQ_000226"
     CDS             34397..35578
                     /gene="setB"
                     /locus_tag="ACHUZQ_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1024037.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35575..35829)
                     /locus_tag="ACHUZQ_000227"
     CDS             complement(35575..35829)
                     /locus_tag="ACHUZQ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1024038.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            35984..36556
                     /gene="yeiP"
                     /locus_tag="ACHUZQ_000228"
     CDS             35984..36556
                     /gene="yeiP"
                     /locus_tag="ACHUZQ_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1024039.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36779..38245
                     /locus_tag="ACHUZQ_000229"
     CDS             36779..38245
                     /locus_tag="ACHUZQ_000229"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1024040.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38363..39349
                     /gene="yeiR"
                     /locus_tag="ACHUZQ_000230"
     CDS             38363..39349
                     /gene="yeiR"
                     /locus_tag="ACHUZQ_000230"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1024041.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39388..40101
                     /gene="lpxT"
                     /locus_tag="ACHUZQ_000231"
     CDS             39388..40101
                     /gene="lpxT"
                     /locus_tag="ACHUZQ_000231"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1024042.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40513..41079
                     /gene="mepS"
                     /locus_tag="ACHUZQ_000232"
     CDS             40513..41079
                     /gene="mepS"
                     /locus_tag="ACHUZQ_000232"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1024043.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41260..42816
                     /locus_tag="ACHUZQ_000233"
     CDS             41260..42816
                     /locus_tag="ACHUZQ_000233"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1024044.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            42898..44712
                     /locus_tag="ACHUZQ_000234"
     CDS             42898..44712
                     /locus_tag="ACHUZQ_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1024045.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44713..45807
                     /locus_tag="ACHUZQ_000235"
     CDS             44713..45807
                     /locus_tag="ACHUZQ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1024046.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45807..46832
                     /locus_tag="ACHUZQ_000236"
     CDS             45807..46832
                     /locus_tag="ACHUZQ_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1024047.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46834..48423
                     /gene="yejF"
                     /locus_tag="ACHUZQ_000237"
     CDS             46834..48423
                     /gene="yejF"
                     /locus_tag="ACHUZQ_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1024048.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(48427..48771)
                     /locus_tag="ACHUZQ_000238"
     CDS             complement(48427..48771)
                     /locus_tag="ACHUZQ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1024049.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(49104..50161)
                     /locus_tag="ACHUZQ_000239"
                     /pseudo
     CDS             complement(49104..50161)
                     /locus_tag="ACHUZQ_000239"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(50252..50947)
                     /gene="rsuA"
                     /locus_tag="ACHUZQ_000240"
     CDS             complement(50252..50947)
                     /gene="rsuA"
                     /locus_tag="ACHUZQ_000240"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1024050.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            51097..52857
                     /locus_tag="ACHUZQ_000241"
     CDS             51097..52857
                     /locus_tag="ACHUZQ_000241"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1024051.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            52982..53266
                     /gene="rplY"
                     /locus_tag="ACHUZQ_000242"
     CDS             52982..53266
                     /gene="rplY"
                     /locus_tag="ACHUZQ_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1024052.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(53405..54412)
                     /gene="yejK"
                     /locus_tag="ACHUZQ_000243"
     CDS             complement(53405..54412)
                     /gene="yejK"
                     /locus_tag="ACHUZQ_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1024053.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54594..54821
                     /locus_tag="ACHUZQ_000244"
     CDS             54594..54821
                     /locus_tag="ACHUZQ_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1024054.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54841..56601
                     /gene="yejM"
                     /locus_tag="ACHUZQ_000245"
     CDS             54841..56601
                     /gene="yejM"
                     /locus_tag="ACHUZQ_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1024055.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56676..56752
                     /locus_tag="ACHUZQ_000246"
     tRNA            56676..56752
                     /locus_tag="ACHUZQ_000246"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56710..56712,aa:Pro,seq:ggg)
     gene            complement(56855..59077)
                     /gene="yejO"
                     /locus_tag="ACHUZQ_000247"
                     /pseudo
     CDS             complement(56855..59077)
                     /gene="yejO"
                     /locus_tag="ACHUZQ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        14542 a        15418 c        14412 g        14705 t
ORIGIN      
        1 gctgccccgg ccttcaggga acgcctcggc aattttgacg ccaccaaaca accgtgcggc
       61 ctattgataa agagctaaca cattgtcaaa aaacatcact atggtttttt agagtttctc
      121 gatatcaatt gcctgaatag cccttgcaat atcaggggaa ttattcaaca cccgaacatg
      181 ctgaaataat tccgttgctt catcgtattc tttacgcaaa taactcaacc actgtttaat
      241 ccgcgcaacg tgatataacc cggtatcgcc ctgcttttcc agacgggtat atttttgcag
      301 caaagcaacc acctccggcc acggcattcg cggttcgtta tattttacta cccggctcag
      361 gttgggaata ttgagcgccc cgcgaccaat catcactgcg tcgcagccgc tgatcgcaat
      421 gcattgttgc gcgctctgcc agtcccagat ttcgccgttg gcaatcaccg gaatattcag
      481 ccgctggcga atctcgccaa tcgcctgcca gtcaatatgc tccgcgcggt aaccctgctc
      541 ttttgtccgt ccatgcacca ccagctccgt agcaccagcc tgttgaaccg catcggcgat
      601 ttcaaatttc ttctcaccgc tgtcccagcc cagacgcact ttcacgctga cgggcaaatg
      661 cgccggtaca gcttcacgca tcgcttttgc accctggtag atgagttcag gatctttgag
      721 taacgtcgcc ccgccgccgc tgccgttaac cgctttcgac gggcagccgc aattgagatc
      781 cacgccccag gaacctaatt ccaccgcacg ggcggcgttc tctgccagcc attgtgggaa
      841 ctgacctaac aactgaacgc gcaccagcgt accggatggt gtccggctgg cgttttgtag
      901 ctcagggcaa atgcgatgaa agacttttac cggcagcagt tgatccacca cgcggacaaa
      961 ctcggtgatg cacagatcgt agtcgttaac ttcggtcagc aattctcgca ccagagagtc
     1021 aagcacaccc tccatcggag ccagtaacac acgcatatct tcacccggaa aaaaatgagg
     1081 cgctatgtta gcgcctatgg tcaacggatt aaaggtctgc aaagagggat tgctgcaaac
     1141 tgtaatccct ctctatgctt gatgtatgcc tgagttcgtt gcgcattgcg taatgccggg
     1201 gagcgaaaaa ataagcgctg atttcttaat gtgatcggta gcacgtttta cgaattaatt
     1261 gtatgatgaa tccatctcat ctggggtgtt gattatgagt aagacactga acattatctg
     1321 gcaatattta cgcgctttcg tcctgattta tgcctgcctg tatgcaggca ttttcattgc
     1381 ttccctgcta ccggtaacca ttccgggcag catcatcggg atgctgatcc tgtttgtcct
     1441 gctggccttg caaattcttc cggcaaaatg ggtcaatccg gggtgctacg tactgattcg
     1501 ctatatggcg ctattgtttg tgccgattgg cgtaggcgtc atgcaatatt ttgatttgct
     1561 ccgcgcacag tttggcccgg tagtggtttc ctgtgcagtc agtacgctgg tggtttttct
     1621 ggtggtgagc tggagttcac aactggtaca cggtgaacgt aaagtcgtag gtcagaaagg
     1681 atcagaagaa tgatggcgaa tatctggtgg tcattaccgc tgacgttgat tgtctttttt
     1741 gccgcccgca aactggcggc acggtataag tttcccctgc ttaacccgtt gctggtagca
     1801 atggtggtga tcattccttt tttaatgctg accggcatct cttacgacag ctactttaaa
     1861 ggtagcgaag tgctaaacga tctgctgcaa ccggcggtcg tcgcgctggc ctatccttta
     1921 tatgagcagc tacaccagat ccgcgcgcgc tggaaatcga tcatcaccat ctgttttatc
     1981 ggcagcgtgg ttgcgatggt gacggggact tccgtggcat tgttgatggg cgcttcaccg
     2041 gaaatcgccg cgtcaatcct gccgaaatca gtcaccacgc ctattgcaat ggcggttggc
     2101 ggcagtattg gcggtattcc ggcaatcagc gcagtttgcg tgattttcgt cggcatcctc
     2161 ggcgcggtat ttggccatac attgcttaat gcgatgcgta ttcgtaccaa agctgcgcgc
     2221 ggtctggcaa tggggacagc ctcgcacgcc ctcggtacgg cgcgctgcgc cgagctggat
     2281 tatcaggaag gtgcatttag ttcgctggcg ctggtgttat gcgggataat tacttcgctg
     2341 atcgcaccgt tccttttccc gattattctg gcagtaatgg gctaaaattt gcgatgcgtc
     2401 gcgcattttt gatgtaagtt tcacgcgttg cataattaat gagattcaga tcacatataa
     2461 agccataacg ggttcgtaaa ctgttatccc attacatgat tatgaggcaa cgccatgcat
     2521 ccacgttttc aaaccgcttt tgcccaactt gcggataact tgcaatctgc actggaacct
     2581 attctggcag acaagtactt ccccgctttg ttgaccgggg agcaagtctc atcgctgaag
     2641 agcgcaacgg ggctggacga agacgcgctg gcattcgcac tacttccact ggcggcggcc
     2701 tgtgcgcgta cgccattgtc gaattttaat gttggcgcaa ttgcgcgcgg tgtgagcgga
     2761 acctggtatt tcggtgccaa tatggaattt attggtgcaa caatgcagca aaccgttcat
     2821 gccgaacaaa gcgcgatcag ccacgcctgg ttgagtggtg aaaaagcgct tgcagccatc
     2881 accgttaact atacgccttg tggtcactgc cgtcagttta tgaatgaact gaacagcggt
     2941 ctggatctgc gtattcatct gccgggccgc gaggcacacg cgctgcgtga ctatctgccg
     3001 gatgcctttg ggccgaaaga tctggagatt aaaacgctgc tgatggacga acaggatcac
     3061 ggctatgcgc tgacgggtga tgcgctttct caggcagcga ttgcggcggc aaaccgttcg
     3121 cacatgcctt acagtaagtc gccaagcggt gtcgcgctgg aatgtaaaga cggtcgtatt
     3181 ttcagcggca gctacgctga aaacgccgca ttcaacccga ctctgccacc gttgcagggg
     3241 gcgttaattc tgttgaatct caagggttat gattacccgg atatccagcg cgcggttctg
     3301 gcagaaaaag ccgatgcgcc gttgattcag tgggatgcca cctccgcaac gctgaaagct
     3361 ctcggctgtc acagtatcga ccgcgtgctt ctcgcttaag cctggtgccg gatgcggcgt
     3421 gaacgccttg tccggcttgc cagccctctc ctggtgtcga aattcccggc aaacagtttg
     3481 ccgtttcttg cgcaaaacca gcgggtaaag tagcctgatg gaaattttcc ttagatcgag
     3541 tctcctgcat gttaaagcgc gtgttcctca gcctgttagt cctgatcggc ttgctgctgt
     3601 tgactgtgct cggcctcgat cgctggatga gctggaaaac cgcgccttat atctacgacg
     3661 aattgcagga tctcccctac cgccaggtcg gcgtggtgct cggcacagca aaatattatc
     3721 gtactggcgt aattaatcag tattatcgct accgcattca aggagcgatt aatgcctata
     3781 acagcggtaa ggtaaattat ctattactga gcggcgataa cgcattgcaa agttataatg
     3841 agccgatgac catgcgcaaa gatttaatcg ctgctggtgt cgacccatca gatattgttc
     3901 tcgattacgc aggctttcgt acgctggatt ccatcgtgcg tacacgcaaa gtgttcgata
     3961 ctaatgattt cattattatc acccaacgtt tccactgtga gcgggcatta tttattgcgc
     4021 tgcatatggg gattcaggct cagtgttatg ccgtaccgtc accgaaagat atgctgtcag
     4081 tacgtattcg cgaatttgcc gcccgtttcg gtgcgctggc tgacctttat atttttagac
     4141 gtgaaccgcg ttttttaggg ccgctggtcc ctattccggc tatgcaccag gtgccggaag
     4201 atgcgcaggg gtatcccgcc gtcacacccg aacagttact tgaattacaa aagaaacaag
     4261 gaaagtagtt atggatgtac agcagttttt tgtcgttgcc gtttttttcc ttatcccgat
     4321 attttgtttc cgcgaagcat ggaaaggctg gcgcgcaggc gcgattgata aacgggttaa
     4381 aaatgcaccg gaaccggtgt atgtctggcg agcaaaaaat cccggactct ttttcgctta
     4441 tatggtggca tatatcggct tcggaatttt atctatcggc atgattgttt atcttatttt
     4501 ctatcgttaa tattttctct tttctgttaa ttattgagaa taattattac ttcactcaat
     4561 aagctgcgga tataacattc tgaaccgcag ctatttgtga atcctttcac agtttaaatt
     4621 tccccacacg cttagcctta atatcagcac gcaatattta tttagcgttc gccttaatta
     4681 ctcatagcat taaggaagat cacatgccgc aacaaaatta tctggatgaa ctcactccgg
     4741 cttttacgcc tttactggcg attaaagaag cctctcgctg tttattatgt catgacgctc
     4801 cctgtagtca ggcttgcccg gcacagaccg atccggggaa atttattcgc tcaatctact
     4861 ttcgtaattt taaaggcgct gccgagacaa ttcgcgaaaa taatgccctc ggtgccgttt
     4921 gtgccagagt gtgcccgacg gagaaattat gccaaagcgg ttgtacccgt gccggtgttg
     4981 atgctcccat tgatatcggc cgcttacagc gttttgttac tgattttgaa caacaaaccg
     5041 ggatggaaat ttatcagccc ggtactaaaa cgctcggcaa agtcgcgatt attggcgcag
     5101 gtcctgccgg attgcaggcc agtgtgacac tgacaaacca gggttatgac gtcacgatct
     5161 atgagaaaga agcacaaccc ggtggctgat tgcgtaacgg cattccgcaa ttccggttac
     5221 cgcaatcagt actggatgca gagatcgccc gtattgaaaa aatgggcgtg accattaagt
     5281 gcaacaacga agtcggtaac acactcaccc ttgagcagct gaaagcagaa aaccgcgcgg
     5341 tactggtcac cgtggggtta tcaagcggtt ccgggctacc gctgtttgag catagtgacg
     5401 ttgagattgc cgtcgacttc ttgcaacgtg cacgacaggc gcaaggcgat atcagcattc
     5461 cacaaagcgc attaattatc ggcggcggtg atgtcgcgat ggacgtagcc agcacgctga
     5521 aagttctcgg ctgtcaggcg gtaacttgcg tagcgcgtga agagttagat gagtttccgg
     5581 caagcgaaaa agagtttacc agcgcccggg agctgggcgt ttcgatcatc gatggattca
     5641 cgccagtagc cgtggaaggc aataaagtca cgtttaagca tgtacggtta tcgggcgaac
     5701 tgacgatggc ggcagataaa attattctcg ccgtcggtca gcatgccaga ctagatgcct
     5761 ttgcggagtt agagccgcag cgtaacacca tcaaaacaca aaattaccag acccgcgacc
     5821 cgcaagtctt tgctgctggc gatattgttg agggagacaa aaccgtggtc tatgccgtga
     5881 aaaccgggaa agaagccgcc gaggcgattc atcactattt agagggagct tgctcatgtt
     5941 aacgaaagat ctttcgatta ctttttgcgg cgtgaagttt cccaacccgt tctgcctctc
     6001 ttcttcgccg gtaggcaact gctatgagat gtgtgccaaa gcctacgaca caggttgggg
     6061 cggtgtggtg tttaaaacga tcggcttttt tatcgccaac gaagtctcgc cgcgttttga
     6121 tcatctggtg aaagaagata ccggttttat cggcttcaaa aatatggagc agattgccga
     6181 gcatccgctg gaggagaatc tggccgccct gcgtcggctg aaagaagatt acccggacaa
     6241 agtattgatc gcttcgatca tgggggaaaa tgagcagcaa tgggaggagc tggcgcgcct
     6301 ggtgcaagaa gctggcgcgg atatgatcga gtgtaacttc tcctgtccgc aaatgacttc
     6361 tcatgcgatg ggtagcgatg tcgggcaaag cccggagctg gtagaaaaat attgtcgggc
     6421 agtgaaacgg ggttccacgc tgccaatgct ggcgaagatg acgccgaata tcggtgatat
     6481 gtgcgaagtg gcgctggcgg cgaagcgcgg cggcgcagat ggcattgcgg cgattaacac
     6541 cgttaaatcc atcaccaata tcgatcttaa tcagaaaatc ggtatgccga tcgttaacgg
     6601 aaaatcaagt atttccggat attccggtaa agcggtaaaa ccgatcgccc tgcgcttcat
     6661 tcagcaaatg cgcacccatc cagaactgcg cgatttccca atcagcggta tcggcggcat
     6721 tgaaacctgg gaggatgcgg cggagttttt attgctcggc gcagcaacgt tacaggtcac
     6781 caccggcatt atgcagtacg gctatcgcat cgtagaagat atggcgagcg ggttatcgca
     6841 ttatctcgcc gatcagggat ttgattcgtt gcaggagatg gtaggtctgg cgaataacaa
     6901 tattgtcccg gcggaagatt tagaccgcag ttatattgtc tatccccgta tcaatcttga
     6961 caaatgtgtt ggctgtggac gctgttatat ttcctgttac gacggcggtc accaggcgat
     7021 ggaatggagc gagaaaaccc gcacaccgca ttgtaatacc gagaaatgtg tgggttgtct
     7081 gctttgtggt cacgtctgcc cggtgggttg tattgagctc ggggaagtga agtttaagaa
     7141 aggcgagaaa gaacacccgg taacgttgta gaacccgcgt cacatccggc atttggttcc
     7201 tgttgccgga tgcggcgtga acgccttatc cggcctacaa aaccacacag atcatgatgt
     7261 tatgtgcgtt tatgaccgaa tgcggaccac attcacatca tttcttacgc gcgtatttca
     7321 gtgaatccag cgctacggcg aagataataa tcgccccttt gatgatgtac tgccagtatg
     7381 ggtttacgcc gatatacgtc aggccatagt tgatgacggt aaaaataatc accccggtca
     7441 ccacgccaat caccgtcccc acaccgccgc tgaacgatac accgcctacc acgcacgccg
     7501 cgatagcatc cagctcatac ataaagccga ggttgttggt ggcagagccg atacgtccgg
     7561 cttctaacat cccgccaaag gcatagaaca cgccagacaa cgcgtagatc atcagcaggt
     7621 tcaggccgac gttgacacca gatacttttg ccgcttccgg gttaccgcca atggcaaaaa
     7681 tgttcttacc gaagcgggtt ttgttccaca acacccagac gaacgccacc gcaatcaacg
     7741 cgtagaaggt gatgtaagag agacggaaac tccccagcgc gacaaagccc tgagcaaagg
     7801 tagagaagcc actgtcaaaa ccagaaattg gcgacgcccc gacaaagtcg taatagagcg
     7861 agttgatgcc atagacgatg atcatcgtgc cgagcgtggt aatgaacggc gtcacgttga
     7921 gataagcgat aatcagaccg ttgatcaaac cgatcaccgc accaatggca cagacaatca
     7981 gaataaccag cgcaatcggc atcgtcgcca tttccgggaa cactttgttg gcgttatcca
     8041 tggactgcaa taatgtcgca gccaccactg ccgccagccc tacctgacga ccagcagaaa
     8101 gatcggtccc ctgggtgaca attaaccctg ccacaccgag cgcgataata atacgcaccg
     8161 atgactgggt gagaatatta cttaagttca acagacttaa aaatgttggg tcctggaaaa
     8221 taataatcgc cagcaaaact aaaagaacga cgtaaatacc gccctctttc aggtaagtaa
     8281 gaaaactttt cttatttaac gcactcatgg gaagccccta atcttaaagg tgcaaagacg
     8341 caagacgcag aatttcgttt tgcgttgttg tttttgtatc gacaattccg gaaacgagac
     8401 cattgctcat gaccagaata cggtctgtta tccctaacaa ctcaggcatt tcagaggaga
     8461 taataataat ccccttgcct ttcttcgcca gttcggcaat taactgataa atttcaaact
     8521 tcgccccgac atcaataccg cgcgtcggtt catcgagcat taatatttct ggttgcgtta
     8581 gtagccagcg accaataatc accttttgct gattaccacc ggagagcgaa ccaatttgcg
     8641 tccgatgacc cggcgttttt acccgcatcg aatcaatcac ccactgggta tcgcttttca
     8701 tccgcgagtt atccagtaaa ccaactttat ttttgtagtt gcgaatattg gaaattaacg
     8761 agttaaaacc aatatccaga taggcataaa ttcccgttga gcggcgctcc tcagttacca
     8821 gtgcaaatcc gtggtttatg gcttcgttgg cattatggtt attgatctgt ttgccgtgca
     8881 acgtaatggt gccagccgat ttctcgcgaa taccaaataa cgtctcaaca atatcggtac
     8941 gtttcgcccc caccagcccg gcaataccga ggatctcccc tttatgcaga tcaaacgaga
     9001 catcgcgaat cgacggctgg cgcagtgacg ttaggttacg cacctcgagg atgacttcgc
     9061 ccggcttgtt ttctttgtca ggaaaacgct gattaagaga acgcccaacc atcatggcga
     9121 tgatcttgtc catcgtcagt cctgccagcg gttcggtggc gatccactga ccgtcgcgca
     9181 atacggtaac ttcatcacat aactggaaga tttcttccat tttatgcgag atataaacaa
     9241 taccgcagcc gcgctctttt aatttacgaa taatagtgaa cagatgattg acctcttttt
     9301 cggttaacga agaagtcggt tcatccataa tcacaatttt cgcgttatag gaaaacgctt
     9361 tggcgatttc gatcatctgc atttgcgaaa cggataatgt gccgacgcgc gcacgcggat
     9421 cgatatcaat atccagttca tcaaaaatcg ctttggtttc gcggtacatt ttgtcctgat
     9481 cgacaaacat gcctttggtg ggatatcgcc ccagccacat gttatccatc accgaacgtt
     9541 gtaataccag gtttaactcc tggtgtacca tcgaaatacc attttccagt gcctctttgg
     9601 cggaatggaa atcgatctct ttaccctgga ataaaatggt gccggagtct ttttgataaa
     9661 taccaaacag gcattttaat aatgtcgatt ttcctgcacc gttttccccc attaatgcat
     9721 ggatagaatg tggccggaat tttaaattaa cgttatcaag tgccttaaca ccaggaaagg
     9781 acttgttgat accgctcatt tccaacaagt attccccgga agacggagtc gttgagctga
     9841 ccatataatt ttaccttgtt ggccatacaa taagggcgca gtaaacgact gcgcccaatc
     9901 agtcttattt cttgctgaat tcagccaggt tgtctttatc tacgccaaca taaggtacgc
     9961 ggaccacttt gttgtcgatt ttccagttgg tgccatcagc cgcacctttg ccatcggcca
    10021 ggtttttcgc cagatcaaag gtcgctttcg cctggttgtt agcatcgttc agtactgtgc
    10081 ccgccagtgc accggatttc accagcgcca gcgcttctgg cagcgcatcg acgccaaaca
    10141 ccggaatgct ggacttgttg tgtgctttca gtgcttctac cgcgcccatt gccatcgcat
    10201 cgttgttggc gataaccact tcgattttgt tggcgttcgg gccagacagc caggcgtcca
    10261 tcttatcttt cgcctgagcg gtgtcccaca ttgcggtatc taactgtaac tgttcagttt
    10321 tgatgccttt atcgttcaat tctttaatca cgtaagtggt acgtgcttct gcatccggat
    10381 ggcccggttc acctttcagc agtacgaact gaatctgacc gtctttgttc agatcccaac
    10441 cctgattcgc cgcccagtgt ttagcaatca aatcgccctg aataatgccg gactctttgg
    10501 agtcagtgcc aacgtagtag gctttgtcgt agctatccag cgccttacga gacggttctt
    10561 tgttgaagaa aaccaccggc acgttttgcc cacgcgcttt ctcaatcacc gtacccgcag
    10621 ctgccgggtc aaccaggttg attgccagtg ccttcacccc tttcgccagc aatacgtcga
    10681 tctgatcgtt ctgcttggac tggtcattct gagaatcatt catcagcagc tgaacatctg
    10741 gcgcggcttt cgcatcttgc tcaatagcct tgcgcactac agacataaag ttatcgtcgt
    10801 acttatagat tgttacacca atgcgagtat cagcagcgtg tgcagcggca ccgaataaca
    10861 tgctggccat cacagcagac agggttaaca ccttcttatt catggtatct ccggtttttc
    10921 ttatgcaggg tagtgcttga gataaatgct cggcggggca gtagagttaa tgaagtgtta
    10981 ctgaacgccg aagctcactt ttttaaattc gttcttccat gcccggtaac gctccagaaa
    11041 acggctttaa ttgttgttat gacgctatta cctcggtgaa agtgattatt cactgttaca
    11101 tacgggttac aacgttaaaa cggtgcaatc atagctatca cattgttaag atactgtgaa
    11161 atcactcaca gattgaaagc ggttacatcg cctgatttgt tgagttagtg atcgccgccg
    11221 cattctggcg cgttgcgaca gaatggcgac gcactaacgt cggcataaaa cagtggctgg
    11281 cacgaggatc aatattgcct gctgccccct gcaaggccag ttcggtggct aatttagcca
    11341 ttgaagcaat gggataacgc acggtcgtta attgcgggtc ggtataacgg gcgatgggaa
    11401 tatcatcgaa accgatgatt gagagatgta acggaatcgc aatgccatta tcttttaatg
    11461 ctgtcagtgc gccagcggcc atattgtcgt tataagcaaa tatagcggta agttgtagat
    11521 tgcgccccag cagttcaacc atcgccgcct caccgcccgg catgtccggc gtaccagtgc
    11581 caatccagct ttccggcgga ataatatcct gctctttcaa cgcactcatc cagcctgctt
    11641 tacgcatggc gtcatcttca atgccgtgac tggaagaaag ataaccaata cgttgatgac
    11701 cgttattcag caacatgcgc gtcgccattc gggcaccgct gagattatcc aggcaaacgc
    11761 aacgatgggc gtaccccggc acaacgcggt tgattaacac cataccggga atgttatcca
    11821 taaattgcgc cagttcatcg tcactcaatg cttttgagtg aacaatcaac gcattacaac
    11881 gctggcgaat taacacctca atggcgtgac gctctttttc cgcttcatga tagctattgc
    11941 cgattagcac gtatttctga tgctgctgag cgactagatc caccgctttt accagcgcgc
    12001 cgaaaaacgc atcagaaacg tccatcacca ccacgccaat ggtgtcgcta acctgagttg
    12061 ccagcgcctg ggcattggcg tttggccgat aatccagctc actcacggct ttcattactg
    12121 cttcacgcgt gtcggcactg accagcgtgc tgttattgag cacccgggaa accgttgcca
    12181 cagagacgcc agcctaacgc gctacatcac gaatggtgat catattcact acctgttatg
    12241 agattgcagt aaatgactgc ttgctggcgg ctattttgtc agcactgaat acaggacttc
    12301 gtgaatcgag tcacagcaat ggaaacggtt acagccgttt tgttaacgaa tgtgatccag
    12361 atcgttatct tgatgtttta gatattgccg ttccggcagc acgcaaagtt aactgacgcc
    12421 agagccattc caccggcccc tggcggaagt aacgcagcca gataacagag aagaggatat
    12481 tcgccagcca taccggaata acaaacgcca gcagctccag gcggtcaaaa tgcataaaca
    12541 aaccgaggtg gtaaaaaagc atggtacaaa tcagcgtttg caatagatag ttggtcagcg
    12601 ccatccgtcc gacgcaggcg atcgcaagca ccagcttaaa gcggctcaat tgcggccaga
    12661 agccataaaa cagcgacgca tagccaatcg cctgaaacgg cgcactcagt tcccgcggca
    12721 tttgaagtaa gaaggcacac cagcgatacg cccagtccag ctgccattgc agggcgatgg
    12781 caggaaggtt aatggtcacc ccaatcgcca ccagcacaaa accagtacga cgatagtgac
    12841 gtaagctgaa ctgccctttc agccagccgc tgcgcatcag cgccgcacca atgagcatca
    12901 tccctgccag ttgccagcca tactgtgcgc ccagtgccag taaactgttg ccaacaccat
    12961 cggcacgatt actgatcgct tcaacgccgc cgtgaagctt ccagtatttt tcatacaaaa
    13021 tagccgatgc atccggcgtc caggtgcggc tggtctggct gtcggaaatc agccccaata
    13081 acagcaaaac gccaagcccc accagataaa gcatgacgcc tgtattaaac aggcttttta
    13141 ccgatggcgc atcgcgcacc agccgccagc agattaagcc caccagcccg taagccagca
    13201 gaatatcgcc gtcccagaac aataaaccgt gaataaaacc cagcaagact aacagcgtta
    13261 accgcgactg gatccagcgt ctgccacggg gcagcaacat ttgcaggccc gcaccaaaca
    13321 gcagcgcaaa aagcgtgagg aatttcacct ggccgatgag atcgagaaat gcccaggtcc
    13381 atgcatcctg cggcgtaata gcgccgtacc aggcgggatt gagataagcc gcctttggta
    13441 gcccaaaggc gctgatgttt aatagcagga tccccagaat ggcgacgccg cgaacaaaat
    13501 cgagcgttac gttgcgctcc atggttcctg ccttttaatc agttgtgatg acgcacagcg
    13561 cgcagaaact cgtggcgcgt attctgactg gatttgaaca atccaccaag agaggtcgtt
    13621 gttgtggcac tggttgcatc gcggatgcca cgcgctttca cgcagtaatg caccgcatca
    13681 atggagacag ccacgttatt ggtgcccagc agcgtttgta gcgcaataag aatttgctgc
    13741 gtcagacgtt cctgcacctg cggacgctgg gcaaagaact gcacgatacg gttaattttt
    13801 gacagaccga tcaccgaatc tttcgggata taagccaccg tcgctttgcc atcgatggta
    13861 acaaaatggt gttcacaggt actggtcaga gtgatatcgc gcacggtgac catttcatcg
    13921 accttcattt tgttttcaat gagggtgatt ttcgggaagt tggcgtaatc cagaccggag
    13981 aaaatttcat cgacatacat tttagcgatg cgatgcggcg tttccatcaa actgtcatca
    14041 gccaggtcga gattcagcag ctgcatgatt tcggtcatat gaccagcaat aaggcttttg
    14101 cgcgtttcgt tatccatttc atgcacgggc gggcgcagcg gtgtttccag tcctcgcgca
    14161 actaacgctt catgaaccag ggccgcttct ttactgagtg atggcattta tgatttctcc
    14221 tgcaggtgtg acgcctccgc cctgcgtggg ggcaaagtta ttaagctgat ttacagcctg
    14281 attattgtgc gtgaggcggc gcacataatc cagtattcac agcgataatt attgtaattg
    14341 ccgctgactt tcatcagcag atgttaaaac atcgttatgc aaatacggaa gtgaaagtta
    14401 ctcacagcac attgaataaa cggtatgatg aagaaattgc aaacaacaca acaaggagcc
    14461 acgcatggaa atgctcgaag agcaccgctg ttttgaaggc tggcagcaac gctggcgaca
    14521 cgactccagt accttaaact gcccgatgac gttcagtatc tttctcccac cccctcgtga
    14581 tcacaccccg ccaccagtgc tgtactggct ttccggatta acctgcaatg acgagaactt
    14641 caccaccaag gcgggtgccc agcgcgtggc ggcagaactg gggattgtac tggtgatgcc
    14701 agacaccagc ccgcgcggcg aaaaggttgc caacgacgat ggctacgatt taggccaggg
    14761 cgcaggcttt tatcttaatg ccacgcaacc gccgtgggcg acgcattacc ggatgtatga
    14821 ttatctgcgc gatgaattac cggcgctggt tcagtcgcaa tttaatgtca gcgaccgctg
    14881 cgccattagc ggtcactcaa tgggtggtca cggtgcgctg attatggcgc tgaaaaatcc
    14941 gggtaaatac accagcgttt cggcctttgc gccaattgtg aatccgtgca gcgtcccgtg
    15001 gggaatcaaa gcgtttagca cctatttagg tgaggacaaa aatgcatggc tggaatggga
    15061 tagttgcgcg ctgatgtatg ccagtaacgc gcaggatgcg atcccgacgc ttatcgatca
    15121 gggcgataat gatcagtttc ttgccgacca gttgcaacct gcggtattgg cagaagccgc
    15181 gcgccagaaa gcgtggccga tgacgctgcg tattcagccg ggatatgatc acagttacta
    15241 cttcatcgcc tcttttatag aggatcacct gcgcttccat gcgcagtatt tactgaagtg
    15301 aaagtccgcc cggttcgccg ggcatcttct catcagaagc gataatccac tgccataaag
    15361 taacgacgtc cgtcttcgtt atagctgtag tcgtcacgac tgagatcctt gtcgccaagg
    15421 ttcagcacgc ctgcgcgcag tttgacgtct ttagtcacct gccaggccgc gccggtattc
    15481 cagatggtat aaccgcccgg tgttttagcc gtcgcgctgt cggcgcgttt ctgcccggta
    15541 tagtgaccag aaacatagaa tgaccagtct tccagcgcca gcggtttcca gtccagcgta
    15601 ccgttagcag tatggaacgg cagatcggat agcggtttgt tttcgccgtt gctgacatca
    15661 cgaccatcgt tgtaggtgta gttgatcgac agtttccatt catcgttgaa cggaattttc
    15721 agttcggttt ccacgccctg aatacgagct ttgttaacgt tgtagtagct aaataccggt
    15781 atgcgccgtc cgttagcgcc cgtctcaaaa ccaacaaagt tttggtagcc cggtgcagcg
    15841 ttgacgtcag acgtacggct gatgctgata cgatctttca catcgttacg gaaaacggta
    15901 acgctggatt caaccccttc cagccagcct tcttcaccca tgtagtaaag ccccagctcc
    15961 caactttcgc tggtttctgg tttcagatcc gggctaccca caatcttaca tgcgccacgg
    16021 caggaattgc tcgtccagtc agggctaagt tgcaacagag aaggtgcttt aaatgccgtc
    16081 gcccagcccc ctttcaccgt tacggtgtcg gtggcgttat aaaccaggta ggcacgcgga
    16141 ctccagtgtt caccgtaggt ttcgtgatcg tccatacgca cgccggtcgt cagcgccagc
    16201 ggctcgaaga tccgccattc atcttccaca aacagcgcgt actggctggc agacgttttg
    16261 gagctggttc ccccggtcag gttcaccgca tcgctaagtt tgtcgtgacg ccattcaccg
    16321 ccaaccgtga gaaactgatt aatcgccgtc agcggcaacg tgtatttgcc gtcgaccgta
    16381 ttgctttcgg aagttatcgg gctgctgttg ccagggtttt tgttctcgac tttctcaccg
    16441 tagtatttca gttcgctggt gccgtaatcc caacgcccat tatggctgac ggagtagttc
    16501 tggcgttcca ggcggttttt gtccagcgag tcggaatcac gatcctgacg gtcgaaaccg
    16561 tatccggcag taaaatcgtg attttgattc ggtgtccagg caaattcgac attgccgtcg
    16621 cggctggaga atccttcaat acgcggcgtt tctccggtat cggtggtcgt tgagttttgc
    16681 gggtcatcct tttcacgttt tgccaggctg ccgtaagctt tcattcccag cacaccatca
    16741 attaatggtc cactggtaaa gaactgaccg ttataggtat caccgcgatc gcgatgttcc
    16801 tgaatggtgg tatcgacggt aacggtaccc gaccatttct gaccgatttt tttggtgatg
    16861 atattcacta caccgccgag cgcatcggaa ccgtacagcg acgacatcgg gccacggacc
    16921 acttcaatac gttcgatgga atcgaccggg atccagttca gatcgaaatc attgtggcgg
    16981 aagacggcat tgcgggagtt cacgcgttta ccgtcgacga gaatcagggt atagctgctg
    17041 tccagaccac gaatactaac gcccttacgg ttatcccctt cgttcgtcag ttgtacgcca
    17101 ggcacttctt tgaggacatc cttcagattc tgtaccggtt ttcgctgcag atcttcctgg
    17161 gtaatgacgc tgatactggc gggtgcatct ttaaggtttt gttcaacgga agatgcagtg
    17221 acaaccatcg tttcgccatc gtcatcgacc gctaacacag gccatacaca agaaatagcg
    17281 gacaaacaca gcccgacccg tacgaaaggg ttcaacctaa acattccata tctccatgag
    17341 gtaactacga aaataaaatg ggtatcgctc acatcttctt tacgtcccct tgcgtacggc
    17401 agcatcgcgg tggacttatt tgtatgcagg tgatcatccc agaaagccgg accagcctct
    17461 gatttgtcag ccttttttga ttgtagcgta acgataatgc aaacgataac agttatcaat
    17521 ccaaatgtta aattttatat ccgcatgatt tgtcagggaa atggaattga aaaagccctc
    17581 tcggttgaga gggcttagca aggaagggag gaaacttatt tcttatcgtt ctgcgggaac
    17641 ttcatttcgc tgtagcgtac gaagtgagtt cctttaatca gcttgtagcc gaaccaaata
    17701 atcaggaaca gcgggatacc aatatacgtt gccgctacgc cgccccagtc aatggtatct
    17761 ttcaggaacg cttcgtagtt ctggcccaaa gtgataatca gacacagaat gaatgcgaag
    17821 atcggcccca gtgggaagaa acctgaacgg tacggcagat cgttaatgtc gtgtccctgc
    17881 aatacgtaac cgcgacggaa gcgatagtgg ctaatggcaa tccccagcca ggcgataaaa
    17941 cccgtcatcc cggaggtgtt cagcagccac aggtataccg tctggttgcc aaacatggag
    18001 gtcaggaagc acaaaccggc aatcaccgtc gtcgcataca gcgcattacg cggcacgcca
    18061 ccacgcgaca atttagcgaa aatgcgcggc gctttaccgt cacacgccag ggtgtacagc
    18121 atacgagtag acgcatacat accggagtta cctgctgaca gcaccgccgt cagaataact
    18181 gcgttcatca ccgccgccgc agagagcaga cccgcgtgct ggaacaccaa ggtgaacgga
    18241 ctgacgctga tgtctttaac atcgttacgc agcaggctcg gatcggtata cggaataatc
    18301 aggctgataa tcaggatcgc gaacacatag aacaacagga ttcgccagaa tacctgacgt
    18361 accgcgcgtg gaatgttttt cgccggatct tcggactcgc ctgcagcaat accgatcagc
    18421 tcggttccct ggaaagagaa gccgacaatc atagctacgc cgatcatcgc cgcaaaacca
    18481 ccagcaaacg gcgcttcgcc gattgtccag ttgctccagc ccgcaggctg cgcgcctttg
    18541 aagataccga taatcatcag cacgccaacg atgataaaga caataactgt cgtgactttg
    18601 atcagtgaaa accagtattc cgcttcacca aagccacgaa ctgagatgta gttcagcagg
    18661 aagataacgc cgaggaacaa cgcactccag atccagcccg gtgtatccgg gaaccaccag
    18721 ctcatgacca gctgtgctgc aaccaagtca acggcaatag tcaccgccca gttgtaccag
    18781 tagttccagc ccagcgcgaa gccaaagcct tcttcaacat agttctgacc gtaagtggca
    18841 aacgaaccgg aaaccggcat atatgcagcc aattcaccga gactggtcat caggaagtaa
    18901 accatcaggc caatcagcat atacgagagc aatgccccgc ccggacctgc ctgagaaatc
    18961 gttgcgccag aggcaacaaa aagacctgta ccgatggaac cgccaatggc aatcatcgtc
    19021 aggtgacgcg cctttaattc acggcgtaag cccggcgctt ctgtagtttt agtttcggaa
    19081 cccatacaaa aatgctatcc atcttaaaaa tgaggcgcga ttgtagcaga cgattggcaa
    19141 tccttccggc agaaatacac ggttataaga caccttcatg atcgcccagg gattataagt
    19201 aaagcagcca atctcttttc tggcgaatgc atgctgaggg cgctgcgtca cgaaatcgac
    19261 gcagcaccag catgttcttg tacagcacct taacgcggta cattcgcggg atcgcaatag
    19321 tccagaaagc gccgtagcgc gttggaaagg tgtttttgcc gatgatgtat acgccacaac
    19381 gtacgcatca ggcgcgggag agggaccgca acttcactta atgtgcctgc ctgcaattga
    19441 tcttcaatca cacgtcgcga caggcaacta attcccaacc catgacgcac cgcatgtttg
    19501 attgcctcgg agttacctaa ttccatcgcc atctcaaact tcggtaaatg tgacagcaac
    19561 agataatcga caatctcccg cgtgccggaa ccgcgttcac gcaggatcca cggcgcagcg
    19621 gccagctgtt ctaaggtgac cggaccacga gccaacggcg aagtcggcgc ggcgaaaacc
    19681 accagctcgt cttccagcca cggttcagaa atgatttcag tgctgtggca cggtccttca
    19741 ataaagccaa tatcaacgcg gaaatccagc accgcttgca tcacgtcctg gctgttccca
    19801 acgctaagtt caatcggcaa ctgcggataa tcatggcgat aacgggcgat aactgcaggc
    19861 agaatgtagt taccgatggt actactggca tagatacgaa tcgcgccgtt gtcttcgcga
    19921 aacagttgtt cgatttcaac cgcctgttca agcaatgcca acgcacgcgg atagagcagc
    19981 cgcccgtgtt cattaacaac cagtcttttc cccacgcgat caaacagttg cacgccaagc
    20041 tgcccttcca ggtcggtcaa ggctgcgctc actgctgatt gcgacaacgc cagcatcacc
    20101 gacgcctggg tggttgatcc acttttcaat acttctgcaa aaacttccaa ctgccggagg
    20161 gtgatgtgca tagtcgctta ccacttataa agattaatta taaatatata atcaatttta
    20221 tttttaaacc agttagtcgt aaccttatgc ccggttaaag gagagggtta tgacgaatat
    20281 caccttacag aaacaacatc gtacactgtg gcattttatt ccggggttag ccctgagtgc
    20341 agttatcacc ggggtcgccc tgtggggtgg ttccattccc gcggttgcgg gtgccgggtt
    20401 tagtgccctc accctcgcaa tcttgttggg gatggtttta ggcaacacca tctatccgca
    20461 catctggaaa agctgtgacg gtggtgtgct gtttgccaag caatatttat tacgtctggg
    20521 tatcattctt tatggcttcc gtctgacgtt ctcgcaaatt gccgatgtcg gtatcagtgg
    20581 gatcatcatt gacgtgttga cgctgtccag taccttcctg ctggcttgct tcctggggca
    20641 gaaagtgttt ggtctggata agcacaccag ctggttgatc ggtgccggta gcagtatctg
    20701 tggtgctgcc gcggtactgg cgactgagtc ggtagtgaaa gcggaagcca gtaaagtaac
    20761 cgtggctgtt gcaaccgttg ttatcttcgg gaccgtcgcg attttcctct acccggcgat
    20821 atatccgctg atgtctcaat ggtttagtcc ggaaactttc ggtatctaca ttggttctac
    20881 tgtgcacgaa gtggcgcagg tggtggcggc aggtcatgcc atcagcccgg atgcggaaaa
    20941 cgcagcagtt atttccaaaa tgctgcgcgt gatgatgctg gctccgttcc tcatcctgct
    21001 ggcggcgcgt gttaaacagc tgtctggggc gaacagcggc gagaaaagca aaatcactat
    21061 tccgtggttt gccatcttgt tcatcgtcgt tgccatcttt aactcgttcc acctgttacc
    21121 gcagagcgtg gtgaacatgc tggtaacgct ggataccttc ctgctggcaa tggcgatggc
    21181 ggcgctgggt ctgaccactc acgtcagcgc gctgaaaaaa gctggggcga aacctctgct
    21241 gatggcactg gtactgtttg cctggctgat tgttggtggt ggtgcgatta actatgtgat
    21301 tcaaagcgtc atcgcataaa ccactacatc ttgctcctgt taacccgcta tcattaccgt
    21361 tttcctccag cgggtttaac aggagtcctc gcatgaaata cattggagcg cacgttagtg
    21421 ctgctggcgg tctggcaaat gccgcaattc gcgccgccga aatcgacgca accgcgtttg
    21481 ccttgttcac caaaaaccaa cgtcagtggc gtgccgcacc gctcacgacg caaaccatcg
    21541 atgaattcaa agccgcctgt gaaaaatatc actacacatc ggcgcaaatt cttccccacg
    21601 acagttatct gattaacctc ggacatccgg tcactgaagc tctggaaaaa tcgcgcgatg
    21661 cctttataga tgaaatgcag cgttgcgaac aactggggct ttctttgctc aacttccacc
    21721 ctggcagcca tctgatgcag atttcagaag aggattgcct tgcgcgtatt gccgaatcca
    21781 tcaacattgc gctggataaa actcaaggtg taacagcggt gattgaaaac accgccggtc
    21841 agggcagtaa cttagggttt aaattcgaac atctcgcggc gattatcgac ggcgtggaag
    21901 ataaatcccg cgtcggcgtc tgcattgata cctgccatgc tttcgctgcc gggtatgatt
    21961 tgcgtactcc agccgaatgc gaaaaaacgt tcgctgattt tgcccgtatt gtcggcttta
    22021 agtatctgcg cgggatgcac cttaacgatg cgaaaagtac ctttggcagc cgcgttgacc
    22081 gccatcatag cctcggtgaa ggcaatatcg gtcatgatgc gttccgctgg atcatgcaag
    22141 acgaccgttt cgacggcatt ccgctgatcc ttgaaaccat caacccggat atctgggcag
    22201 aagagatcgc ctggctgaaa gcgcaacaaa ctgaaaaagc ggtggcctga tcgatgaacg
    22261 acagggaaaa gcagattctt aaaattttac ggcgtaaccc gctgattcaa cagaatgaaa
    22321 tcgccgatat tctgcaaatc agccgctcac gtgttgccgc tcatattatg gatttgatgc
    22381 gcaagggttt gattaaaggc aaaggatata tccttaccga gcaggattac tgtgtggttg
    22441 tgggtgcaat caatatggat attcgcggaa tggcggatat ccgttacccg caagcagcct
    22501 ctcatccagg ttctgttcac tgttccgcag gcggcgtcgg acgtaatatc gcgcataacc
    22561 tggctctgct tggtcgggat gtgcatttaa tatcagctat tggcaacgat ttttacggcg
    22621 agacattact ggaagaaacg cgccgcgcgg gcgtgaacgt ttcaaactgc attcgtctgc
    22681 atggacacag cactgcaact tatctcgcta tcgctaataa gcaggaagaa actatcctgg
    22741 caatcaatga cacacatatt ctgcaacagc taacgcctca actgctcaat acttcacggg
    22801 acttgattcg ccatgctggt gtggtgctgg ctgattgtaa tctcacacct gaagccctgg
    22861 agtgggtctt taccattgct gatgaaattc cgatgtttgt cgataccgtt tccgaattta
    22921 aagcgaacaa agtaaaaaac tggtacagcc ggatccacac tctgaaacca acacaaaatg
    22981 aactggaaat tttgtggggg caaccgatta aagacgacaa cgatcgcatc agagctgtaa
    23041 attcgctgca tcaacaaggt gttaaacgca tttttgttta tctgaaagat gaatctgtat
    23101 tttgcagcga taaggatgga gaacaatttt tgctgaccgc gccagcgcat acgacagtag
    23161 acagttttgg tgctgacgat ggttttatgg cgggcctggt atatagcttt cttgaaggat
    23221 acagtttccg cgacagcgcc cgttttgcgg tagcctgcgc ggcaatttcg cgcgccagcg
    23281 gcagcttaaa caaccctacc ctgtctgccg ataacgcgct ttcattagtg ccaatggtgt
    23341 aacaatgttg ccggattcga cgcttgaccg catcttatcc ggctcataaa ttgcgcacta
    23401 cgctaaacca ataaagaatc ctgcaatagt agcactcatc aggttagaaa gtgtcgccgc
    23461 cgccagcgcg cgtaaaccaa gttgggcgat ttccggcgca cggtgtggcg caaccgcaga
    23521 aaacgccccc accaccaccc cgatagaacc aaagttagcg aaaccgcaca acgcgaaaga
    23581 aataatcgcc acggttttag catccagagt gccacccgtt tgcagatatg gcgagaaatt
    23641 gagataagcg acaaattcat tgatcgccag cttctgccca atcaaactcc cggcaagatt
    23701 tgcatcactc cagtcaaccc ccatcaccca cgccaatggg gccaacaggt aacctaaaat
    23761 cgactccagc gaagcatgtt caaaaccaaa ccagccgcca acaccgccga taataccgtt
    23821 aatcaacgca attattgcaa caaatgccat aacaactgtc gctacaccgg cggcgatttt
    23881 cagcccggtc attgcccctg tcgcagcggc ttcaataatg ctttttggcg gtgtttcggt
    23941 gaaagagagg ttattaaaag aaacctgtga agattccgta gccgggctta acaggcgggc
    24001 aaacaagatc ccgccaggga tcgccattaa cgatgccgcc agcaaatatt ccacaggtac
    24061 gcccagtgcg gcgtaaccaa tcattgtcga accagcaatc gaggccatgc cactacaaat
    24121 cgctgtaaat aattcattgc gattcagacg atcgataaag ggtttgacga tcgccggaat
    24181 ttcgttttgc ccgaggaaaa tggtggtgac cgcgacgaat gactcgatct tgctgatatt
    24241 taaagctttc tggaagatac cgccgagaat gcgaattaag atccccatca caccgatgta
    24301 gtagagaata ctcaccagtg cagtgacgaa gataattgcc ggtaataccc tgaaaccaaa
    24361 gataaatcct gcgccatcaa ataaggtatc cattttcggt ccgaccagag aaccgaagat
    24421 aaatgcgcta cctgcgtcgc tgtacgccat cactttgtgg acgccaaaag cgactttttc
    24481 tgcgacccaa cgccctggcg gtaaccaaag cataatcccg ccaatcacga cctgtaacac
    24541 taacgccgcg ccaacggtac gcaggctgat cttcttcttg tttactgaca gtaaaaacgc
    24601 aattgccagc agtaccacca ttcccagaac acttctcatg acatccatag tgattatctc
    24661 ttcatgtcat tttgggcgac ggctttctgc ccatcagaaa tagccgggcc tgcatcttgc
    24721 gatactgtcc cggcttgtta tcaacactta atgggttttt atgtagccgc gtacgcactc
    24781 ttcgactaat ccccagaacc agtcggtatc aatagtgatg ccgactttag tattggcggg
    24841 cttgccaaga acgcccagct cgtcgcagac ggtacgccca tagcaagggc cactgttgac
    24901 gtccacttcg acgtacatct cctgggtttt aatgccatca gggttaatca gataaccgat
    24961 gcaggtggcg tcgtgcaccg ggccgccagc caggccgtag ttttcgaact gcgttttgag
    25021 agtgaagttc atgatgtcgc tgaacatctc tccggcaggg ccgcctgccc tttccattcg
    25081 tgcaattacg tccggggtgc aaacggtctg gttggtaaga tcgaggccca tcatcactaa
    25141 tggaacgccg gaggtgaaca ccacgcgtgc ggcttccggg tcggcaaaga tattgaactc
    25201 ggcagatggg gtgaagttgc ctgtaccgta agcgccgccc attagcacaa tttcacggat
    25261 tttgggcagg attgcgggtt gcatacgcat tgccaccgcg atatttgaaa gcggaccaac
    25321 cggcaccaga gtgatatcgc catcgcttgc cataagggta tcaatgatgt atttcaccgc
    25381 gtgggtactt tctgcctggc gggtcagcgg ctcgaatacc gggccatcca gtccggtttc
    25441 accgtgaata ttatcggcaa cgatttgttg acgcataatg ggttgcggca tccccgcata
    25501 aaccggaaca ttaatctcca gtttctggca aacattcagg ccattaatta atgttttatc
    25561 aagcgtctga ttacccgcaa caatggtgat acctaataaa tcaatcgccg gatgtttcgc
    25621 cgccatcatt atagcaatag catcatcatg acccggatca caatccagaa taatttttct
    25681 cttttccatt gtttatttcc tctgtttcca gttgcgttat tttttctaca gcaacgaaat
    25741 ttcgatcggg ccgatgattg aatcttaaca acagcgtacg aatgctaaat atgagaaatc
    25801 tcatagcgga taaacatcgt gaaagaaatc cacaataatg atcttaagca gcaattgatg
    25861 agtgaatccg cgtttaagga ttgcttttta acggatgttt cagccgatac gcggctgttt
    25921 cattttttag cgcgtgacta cattgtgcag gaagggcaac agccgtcctg gctgttttac
    25981 ctgacgcgag gccgcgccag gctttacgcc acgctagcta atggtcgcgt gtcgctgatc
    26041 gatttctttg ccgccccctg ttttattggc gagattgagt taatcgacaa agaccatgaa
    26101 ccgcgtgcgg tgcaggctat tgaagagtgt tggtgccttg cgctccctat gaaacattac
    26161 cgcccgctgt tattaaacga cacgctattt ttacgaaaac tctgcgtcac cttaagtcat
    26221 aaaaattatc gtaatattgt ttctttaact cagaatcaat catttccgtt agtgaatcgc
    26281 ctggcagcat ttatattact ctcgcaggaa ggtgatcttt atcacgaaaa gcatacgcaa
    26341 gcggcagagt atttaggcgt ttcttatcga catcttttat atgttctcgc gcaattcatt
    26401 cacgacggtt tattaattaa aagcaagaaa gggtatctaa ttaaaaacag aaagcagttg
    26461 tcaggactgg cgctggagat ggacccggag aataaattct ccgggatgat gcagtaaaaa
    26521 ttattggcaa tagcacgatt gccggatgca acgcttaata aggcgtacat ccggcaatga
    26581 aactcaagct aaaccaataa agaacccggc aatggtcgcg ctcatcaggt tggaaagcgt
    26641 cgccgccgcc agcgcccgta aaccaagctg ggcgatttcc ggcgcgcgtt ttggcgaaat
    26701 agccgaaaac gctccaacga caacaccgat agaaccaaag tttgcaaacc cacagagggc
    26761 gaacgagata atggcaatgg tcttgacatc cagcgtgccg ctcgtttgca gatagggcga
    26821 gaaattgaga taagcaacaa actcattaat cgccagtttc tgcccaatca aacttccggc
    26881 aagatttgca tcactccagt ccacccccat aatccatgcc aacggggcca gcacatagcc
    26941 gaaaatgctt tccagagaga cattggcaaa accaaaccag ccgccaactc caccaataat
    27001 gccgttgatc agcgcaatga tcgctacaaa tgccattacc accgtcgcca caccggcggc
    27061 gattttcagc ccggtcattg caccgttcgc cgcagcttca ataatgcttt ttggcggtgt
    27121 ttcgctaaac gacagatttt caaacgtaac ttgcgaaggt tccgtcgccg gactaagaat
    27181 acgtgcaaac aggataccgc caggaatagc catcagcgaa gcggctaaca aatagtcaat
    27241 cggcacgccc attccggcat agccaatcat catcgaaccc gcaatagacg ccatgccgct
    27301 acaaatagcc gtaaatagct cgttgcgatt catgcgatca ataaaaggtt taacgatcgc
    27361 cgggatctca ttttgcccga ggaaaatagt ggtgactgcg acaaaagatt cgattttgct
    27421 gatgttgagg gctttctgaa aaatactgcc aagaatacga atcagcagcc ccattacgcc
    27481 aatgtagtac agcaagctga taagcgcggt aacgaaaata atcgccggga gtacgcgaaa
    27541 ggcgaagata aagcccgcac cgtcaaacag aacatccatt ttgggaccga ccagcgaacc
    27601 aaaaataaag gcacttcctg cgtcactgta ggacatcact ttatggacgc ctaatgccgc
    27661 ctgctctact gcccattttc ccgggggaaa atagagcatg atgccaccaa tggcaatttg
    27721 cagcagcagc gcagcgccaa ccgtgcgcaa gctgatgctc tttttattta ccgacaacac
    27781 aaaggcaatt gccagcaata ccaccatccc cgcaacactt ctcattatat ccataatgat
    27841 tttcccttca tgccagtaaa cccggcgtca gcgccaggtt ttggtatgtt tgatgagtac
    27901 gggcgacggc tttctgcccg ccagaaatta acccacgaga cgctgatatt ctttggcaat
    27961 ttcgctcgcc agaatggcgt tattaaagac cagttgaata ttggatttca ggctatcacc
    28021 gccggttagc tccgcaacac gcgccagcag gaacggtgta ctctctttcc caataacacc
    28081 ctgttcttcg gcttcagcaa cggcttgatc aattgctgca ttgatggttt cttctggcat
    28141 cgcaaactgt tccgggatcg ggttcgctac caccaggccg ccgttcaaac cggtttgcca
    28201 cttcactgcc atcgcgcgag caatttcgcg ggcactgtca agacgaatgc tgacttcaaa
    28261 tgggctggtt cggcagaaaa acgcaggcag cgcttttgtc tgataaccaa ttaacggtac
    28321 accgaaggtt tctaaatatt ctgttgttaa tcctaaatcg agaatagatt ttgcccccgc
    28381 acaaacaaca gtgacattgg tatttgccag ttcctgtaaa tcggcagaaa tatcgaaggt
    28441 atgttccgct ccgcgatgca caccaccaat acctccggtt gcaaaaactt taatcccagc
    28501 cagtgctgca ataatcatgg ttgatgcaac ggtagtcgcg ccatttttcc ctgcagcaac
    28561 gacaaaaggt aaatcacgac gactaacttt cgtcacgtta tgtccttcac ggcccagcaa
    28621 ttcgatttct tctttactta agcccacttt catcacaccg ccaataatgg cgatagtggc
    28681 tggtactgcg ccctgtttac gaattgtttc ttctacttca attgcggttt gggcattttg
    28741 tgggaatggc attccgtgag aaattatggt cgattccagc gcaacaaccg gttttttatt
    28801 ttttaaagca tcctgtactt cagcggatat ttgtaataat tcaggggaca gagttaattc
    28861 agacattctg tgttctccac taacgatcta acattggcaa ttgataattc agggttgttg
    28921 gtatattcac aggcaagtgc cattgacgaa catccctgcg caaatcgaac agaatcgata
    28981 aacggcatac cgtctaccca acaggaggcc agccccgcca tcatggcgtc gcctgcccca
    29041 gtaacattga tgacattcgt tttaataggg gccgaccacc cactttcacc gttgatatca
    29101 ctgtagtaaa cgccatcccc tcccatgctc agcaccaagc ggttcaggcc gtgttgatga
    29161 aaccaggcgg cgacctttgc gacatcttca cgtccggaaa gtgcaattcc gctcaacgtt
    29221 tcggcttcaa gacggttagg ttttaacgta tggattttgc tcagatgatc gcggattttg
    29281 acgcatttcc atgccgatac cggatcaata aatacaggcg tttccccgct attttccaga
    29341 acccagacca gcgcctcttc actaaggttg caatccgcga caataacctt tgcaccgcga
    29401 ataaactcct gatgctgggc aagaaattct gcagaaatac agtcactaat actcatatca
    29461 tttatcgcga caagcatttc tcctgtatta tcgagcagtg ataaatagct ggatgtattc
    29521 tcacccggaa caattaagca tttatcgaca taaacaccgg attgattagt ttgcgccagt
    29581 aatgattgcc cataaaaatc accacctact gcacttaaaa gccaggcatt tttccccagt
    29641 aacgccagat tatgagcgat attccttccc acgccacctg gggtaaattt aatttttccg
    29701 ggattcgaat ccgcataatt caatggatga tgtgaatacc cagccacatc aatattcgcc
    29761 gaaccaataa ttactatgta gtccttttcg cgcatagcac gccccctggc aataaattgc
    29821 caacgaagaa attgggtaaa atagccatta cagtcaataa gactattaga gcacgtgttc
    29881 agatttaaac acatgttcat aataagcata tttaacgtaa tgtaaatcga tcagaaaaaa
    29941 cgaaatcggc cagatatgaa ggcggtcaca aagtttgtct atttatgcag caacaaaaga
    30001 gaaagttgtg atgtgtaaca cacaatatgg caggcaagaa aaagggcaga gatgtttctg
    30061 ccctccagat tgttgacaac gcgcgctttg ttcatcctgg atgtggcgta aacgccttat
    30121 ccgacctaca aaagcgcaaa aaattcaata tattgcaggg ttcatgtagg tctgataagc
    30181 atagcgcatc aggcaatttt gcgtttgtca tcatcggggc agagatgtct catccccgat
    30241 aatgaagata aattatgcgt ttttttctac tatttgtgtt tccgggcgtt tcaggaaggc
    30301 ataggccaaa cccgccacca gcgtaccggc aataattgct accaggtaac ccaataccgg
    30361 cgtaatagcg ccagggatca gcagaacaaa cagaccaccg tgtggtgcca tcagtttcgc
    30421 accaatcgcc attgagattg cgccagtcag cgccccacca acgatacagc acggtagcac
    30481 acgcatcgga tcacgagcag caaacggaat tgcaccttcc gaaataaagc acagaccaag
    30541 taccagagcg gctttgccac cttcctgctg cgctttgtcg aatttgcgac gcgccaccat
    30601 tgttgccaga cccattgcca gcggtggcac catacctgcc gccataatcg ccgccatcgg
    30661 gccataggtt tgagtactca gcagacccac accgaatgcg tacgctgctt tgtttaccgg
    30721 accgcccatg tcagtacaca tcatgccacc gaggatcgcc cccagcagaa ccgcattcgc
    30781 agtccccatg gtctgcagcc agtgagtcaa cccatcgaga atgccagcaa ccggtttacc
    30841 gattaggtag atcatcgcca gaccgaccac cagactggaa attagcggaa tgatcaggat
    30901 cggtttcagc gcctccatac tctgtggcag tttcaattgc gtactgatta acttcgcaat
    30961 gtaaccagcc aggaagccag caattatacc gccaataaag ccagaaccgg tgctgaccgc
    31021 cagcataccg ccaatcagac ccggtgtgag gcccggacga tcggcaatgg agaaggcaat
    31081 ataacctgcc agtaccggca ccatcagcgc aaaggctgaa ccaccaccaa tctgcatcag
    31141 cgccgctgcc aacgtacccg gctctttaaa cgcttcgata ccaaaagcaa aagaaagcgc
    31201 gatacacaga ccacctgcaa caaccatcgg cagcatgtaa gagacgcccg tcagcaagtg
    31261 acggtaagcg cctgcactct ctttcttacc ttcagtggtc gctgtttgag ctttgcccgc
    31321 tggttcatac ggcgttgctt cagcaaccgc tttatccagt tcctgcgcag ttttcttcag
    31381 cgccagaccg gtagaggtgc gatacatcgg tttaccagca aatttcgcca gatccacttc
    31441 gatatctgcc gccacaatca ccagatccgc tgctgcgact tcttcagggg tgattgcatt
    31501 gcccgcgcca acagaaccac gggtttcaac tttcacccac cagccacgtt ttttcgcttc
    31561 ggtttcaatg gcttcagccg ccataaaggt gtgtgctacg ccagtcgggc aagcagtcac
    31621 cgcaactacg cgtttcggac cgctggcagc aaccggtgct gtcgcagcga ccggcgcagt
    31681 gtaaggtttc gcatggcctt tggcttcact caggaacagc tcagggtgcg caactgcccg
    31741 ggaaatatca cccagccaga catttttacc gttcagcgcg ctgtcattcg ggatggaatc
    31801 accgagaaca atcgccattt ctgcgtcgtt cggattgtcg atgatttcca gttttgcttt
    31861 tcgcgccgcc gcgcccagca gggtcttcgc catataggcg cgtgcctgac cgagattagc
    31921 gtcaataatc agcagcgttt tcattatgcc tctcctgctg tcagttaaaa ggttgtaagt
    31981 cgacgcgcgc catcattgcg gccaactgcg gacgatcggt aatacccaca ttgctttgac
    32041 ttaccgccag ggctgcaaca gctgtcgcca gacgcagtgt gtgttcactg gattcacgca
    32101 tcagcaagcc ataaatcagg ccaccaacca tagaatcccc tgcgccaacg gtgcttacga
    32161 catcgactga cggtggtttg gcgatccatt cgccggaggc attaacccaa agcgcgcctt
    32221 cggcacccag tgaaataaca acatgcgcaa taccttgttc acgtagcgca tgtgcagctt
    32281 caatcacatc tttcatttca ggcagtttac ggcctgccca gatttccaac tcgcggcggt
    32341 taggtttcac cagccacggt gccgctttca aacctgctac taacgcttca cggctactat
    32401 caaagataat gcaaggacac tgactacgca ggcgagtcat ccagtcggtg aacgcttccg
    32461 ggctgacgcc tgacggtaag cttccgctga cacagaccat atcgaactga ccgagccagc
    32521 tcagagaatc agtcacaaag cgttcccagt cggcgagggt gacttcaaaa cccgagaagt
    32581 tgaagtcggt cacttcgccg tctttttccg tcagcttaac gttaattcgg gtgcgcccct
    32641 gtacaacctg gaaacggttg gcaatgccca gctcgctgaa cagttgctga aaaccatcct
    32701 gattgtcttt acccaggaag ccgccaacgg tgacatcaat tcccaggtct tttaatactt
    32761 tggccacgtt gatgccttta cccgccgcat gcagaccggt ggttttcacc aggttcactt
    32821 cgccgcgttc aatttccggg cagaaaccaa caaggtcata agccggatta agggtgatcg
    32881 tagcaacacg tctgctcatt atgcgccctc cccaagacca gcagcgatag cgtcgccaat
    32941 ggctttcagc gcctgttcag catctgcacc ctgggcggta aagcgtaggc gatgaccttt
    33001 cttaacgcca agtgccacaa ctttcatcag actacgtccg tttgccggtt tgccggtgcc
    33061 atcaaggttt gtcacggtaa tatcactgtt aaattgttta atggtattga ccagcatggt
    33121 acctggacga gcatgcaggc cgtgttcatt gcgcaccaca aactccgcgc ttaacacgtc
    33181 gtcggtcggc gcatcatcgc tggtcagcag cgccagcaac gttgccgcat ccgctttcag
    33241 caagcggtca gctttattgt cgagcaataa atcagcgaga cgcttaagaa ccgcgatggg
    33301 ctgatcgtca ttcatcgcca cactcaccag catggctgcc gtttcgccgt ccacatcaaa
    33361 agcatttgcc gcacggctta ccgcaatcgc gctacgcaga ttgccttcgg cgctatcgct
    33421 cagccagata ccctgtccga gattcagcgg ttgttcattg atggctttgg tgacgaaagt
    33481 ggcgtcaact gcccccacct ctttcagacg cgcagcgttc agcgcctgaa gagtcagcag
    33541 atcgctggcg acgatatcca gcgtcagcat ttcgttgtcg agcttcagct gctcactctg
    33601 cttttcgccc atcagtaatg cgcgaagttc ttctgctgtt gttgctgact tcagttgttc
    33661 agcaacggaa tcatcgctca gtacgtgggt cagctggcgt agcaggccca gatgttcatc
    33721 cgagctggca gcaataccga ttgccacgta cgctacctga ccgtcacccc aggtgacgcc
    33781 ttccgggaac tgaaatacct gaacgccggt tttcagcacc tgatcgcggg tgtcggtagt
    33841 gccgtgtgga atagcaatac cattgccgag gaacgttgag gtttgctgtt cgcgcgccag
    33901 catgccattg acgtagcctt ctgctacatt accggcctgc accagcgccg cagcgacctg
    33961 gcgaatcgcc tcttctttgt ctccggcctt ttcgcccgga tggatgtcct gtacggataa
    34021 ctggaacata gttctcctct cttgctgaat tgaaacgatt cagcctctat gagaaaaaaa
    34081 gcgccaacct ggcttagggt taaagacaag atcgcgctga aacgtttcaa gaaagcataa
    34141 tacttctgtt tcagcacgca aggaaagccg taaagttatg agcaaaagtt tgatgtgctg
    34201 cacaattttt tgtcattttt cctaattgct gatgggaaaa tccgttatca gcaatttcat
    34261 ttcagcagcg tcagcagcca atcagcaaca gtgaaatggc attttgatta ttcgtggcgc
    34321 ttttgatttc gctgtctgtt tattttctga caagcagcgt aaactccgcg tcttcctctt
    34381 ccagtgatcg acctgcatgc ataactcccc cgcagtctcc agcgcgaaat cgtttgacct
    34441 gacctcgacg gcgtttttaa tcgttgcctt tctcaccggt attgcgggtg ctctgcaaac
    34501 cccgacactc agtattttcc ttaccgatga agtacatgcc cgtccggcga tggtgggatt
    34561 cttctttacc ggcagcgctg tcattgggat tctggtaagt cagtttctcg ctgggcgctc
    34621 tgataagcgc ggcgatcgca aatcgctgat tgtcttttgc tgcctgttag gcgtgctggc
    34681 ctgcaccctt tttgcctgga atcgcaacta ctttgttttg ctattcgttg gcgtctttct
    34741 tagcagcttt ggctcgaccg ctaacccgca aatgtttgcc cttgcccgtg aacatgccga
    34801 caaaaccgga cgtgaggcgg tgatgttcag ctctttttta cgcgctcagg tttcactggc
    34861 atgggtcatt ggcccaccgc tggcttatgc gttagcgatg ggtttcagct ttacggtaat
    34921 gtatctgagc gcagcggtag cgtttattgt ttgcggcgtg atggtgtggc tgtttttacc
    34981 gtcgatgcaa aaagagcttc cgctggcgac cggcacggtt gaagcgccgc gccgtaaccg
    35041 tcgcgatacg ctactgctgt ttgtcatttg tacattgatg tggggctcga acagcctgta
    35101 catcatcaac atgccgctat ttattatcaa cgaactgcat cttcccgaga aactggccga
    35161 tgtgatgatg gggaccgccg ccgggctgga aatcccgacg atgttgattg ccggatattt
    35221 cgccaaacgt ctgggtaagc gtttcttaat gcgcgttgct gccgtgggtg gcgtctgttt
    35281 ttacgcagga atgctgatgg cgcattcacc tgtcattctg ttgggcttgc agctgctaaa
    35341 tgctattttt attggcattc tgggcggcat cgggatgctc tattttcagg atctgatgcc
    35401 cggtcaggcg ggttcagcca ccacgctcta taccaacact tcgcgcgtgg gctggatcat
    35461 cgcaggatca gtggcgggca tcgtcgccga aatctggaat tatcacgctg tgttctggtt
    35521 tgcgatggtg atgattatcg ccactctgtt ttgcttactg cggattaaag atgtttaagg
    35581 cgcggtcagc atttccagat cgataagcca ggtcatggcc tgctgacgcg agttaccgca
    35641 catttcagcg ctggcttgta agcctgcaca gactttcggt cgcagtggcg aggtgaaaat
    35701 tttacagcgt tgctgttcat caagctgaat gcagggcgta ttggcgggct tgccatctgg
    35761 cataccggga atagggctgg aaattgaagg ggcagtgcaa cacgccccgc aacccggacg
    35821 gcattccata cgttgttctc ttaaggttaa ccaccggatt gatgagcgcg cacagtacca
    35881 ccttttttgc accagcaaaa gtgcgaatac cacttgcccg aaaggcccgt cgcgagtact
    35941 ttgtcgcgat atttttgaca ttttcgacta caggaatttt tcgatgccaa gagcgaacga
    36001 aattaaaaaa ggtatggtac tgaattacaa cggcaaactg ctgctggtta aggatattga
    36061 tattcagtcg cccactgccc gcggtgccgc tacgctgtac aaaatgcgtt tttctgatgt
    36121 ccgtaccggg ctgaaagtag aagagcgttt caaaggtgat gatatcgttg acaccgtgac
    36181 gctgacccgc cgttacgttg acttctccta tgtcgatggc aacgaatatg tctttatgga
    36241 taaagaagac tataccccgt ataccttcac caaagatcag attgaagaag agttgctgtt
    36301 tatgccagag ggcggcatgc cggacatgca ggtgctgacc tgggacggtc aactgctggc
    36361 gcttgagctg ccgcagaccg ttgatctgga aatcgttgaa accgcaccag gcatcaaagg
    36421 ggcatctgcc agcgcccgta acaaaccggc gacattgagc actggtctgg tgattcaggt
    36481 accagaatac ttaagcccgg gcgaaaaaat tcgtatccat atcgaagaac gccgttatat
    36541 ggggcgtgct gactaacttc agccgcatgc agaaaaggga tagctcaggc tgtccctttt
    36601 ttaatttatt ataccaatct tctattttgc gcttcctgaa caccttacgc cctcaatttt
    36661 cactcgttga ttgatcgccc tcacacttca tcgcattaac aatccagacc aatttcaatt
    36721 gctgtcatat aactttacac tgtcattgtt aatttatcgt tactaagacg tgactcctat
    36781 gaagacaatt gcctccgtta cgctcccgca tcatgtacac gctccacgct acgatcgcca
    36841 gcagttgcaa tcacgtatcg ttcattttgg ctttggcgcc tttcaccgcg ctcatcaggc
    36901 gttactgacc gatcgtgtgc tgaatgccca gggcggcgac tgggggatct gtgaaatcag
    36961 cttgttcagc ggtgatcaac tgatgagcca gctccgcgca cagaaccatt tatataccgt
    37021 gctggagaaa ggtgcggacg gcaatcaggt gataattgtc ggtgccgttc acgaatgcct
    37081 taatgcaaaa ctggattcct tagcggcaat tattgagaaa ttttgcgagc cacaggtggc
    37141 aattgtttcc ctgacgatta ccgaaaaagg ctattgtatt gacccggcca ccggtgcact
    37201 cgacaccagt aatccgcgga ttattcacga tctacaaacc cctgaagaac ctcactccgc
    37261 accgggtatt ctcgtcgaag cactgaaacg ccgccgtgag cgcggcctta caccgtttac
    37321 cgtgctctcc tgcgacaata ttcccgacaa tggccatgtg gtgaaaaacg cggtgctggg
    37381 aatggcagaa aaatgttcgc cagaactcgc cgggtggata aaagaacacg tcagttttcc
    37441 gggaaccatg gtcgaccgca ttgttccggc tgcaaccgac gaatcactgg cagaaatcag
    37501 ccagcatctg ggggtaaatg atccctgcgc gattagctgc gaaccgttta tccagtgggt
    37561 ggttgaagat aacttcatcg ctgggcgtcc tgcctgggaa gtcgcaggtg tacaaatggt
    37621 gaatgatgtc ctgccatggg aagagatgaa actgcgtatg cttaatggca gccactcttt
    37681 tctcgcttat ctgggttacc tctcaggatt cgtccatatc agtgattgca tgcaggatcg
    37741 cgtatttcgc catgctgcca gaacattaat gctggatgag caagcgccga cactgcaaat
    37801 taaagatgtc gatttaacac aatatgcgga taagttaatt gcacgttttg ctaatccgtc
    37861 gctgaaacat aagacctggc aaatcgcgat ggatggcagc cagaaattac cgcaacgtat
    37921 gctggcaggt attcgcatac atctggggcg tgaaacggac tggtcgttgc tggcattagg
    37981 cgttgctggc tggatgcgtt acgtcagcgg cgttgatgat gccggaaatg ccattgatgt
    38041 tcgcgatccg cttagcgata aaattcgcga acttgttgcg ggcagcagca gtgaacaacg
    38101 cgtaaccgcc ctgctttccc tgcgtgaagt tttcggtgat gatctgccag ataacccgca
    38161 ttttgtgcag gccatcgaac aagcctggca acaaatcgta caattcggcg cacatcaggc
    38221 gctattaaac accctcaaaa tttaacgatt tctgcggtta aagcggatga agctcacctt
    38281 cgtccgctct ccccttctct tttctgcctt ttttagccag gattaacgct cagttaactt
    38341 accagagtat tgcggagccg ttgtgaccag gaccaacctc atcaccggtt ttctcggcag
    38401 cgggaaaacc acgtcgattc ttcatctgtt agcccataaa gatcccaacg aaaaatgggc
    38461 ggtactggtt aatgaatttg gggaagtcgg aattgatggt gctttgctcg ccgatagcgg
    38521 cgcattgctg aaagagatcc ccggcggctg catgtgctgc gttaatggtt tacccatgca
    38581 ggtagggttg aataccttac tgcgtcaggg aaaaccagac cgcttgttga tagagccgac
    38641 cgggctgggc catccgaaac agatcctcga tctgttaacc gcaccagtct atgaactgtg
    38701 gatagatctg cgcgccacct tgtgcattct cgatccacgc ctgctgctgg acgaaaaaag
    38761 cgccagcaat gaaaacttcc gtgaccagct ggctgccgca gacatcattg tcgccaataa
    38821 atccgaccgt gcgacgcccg aaagtgagca agcgctacag cgttggtggc agcaaaatgg
    38881 tggcgatcga caattaattc acagtgagca tgggaaagtt gacggtcatc ttctggattt
    38941 gccgcgtcgc aatttagccg agttgcccgc cagcgccgcg cattctcatc agcatgtcgt
    39001 gaaaaaaggg ttagcagcgt taagcctgcc agagcatcaa cgctggcgtc gcagtctgaa
    39061 cagcgggcaa ggatatcagg cctgcggctg gatattcgac gctgatacgg tattcgacac
    39121 cattggcatt ctggaatggg cgcgacttgc accggtggaa cgcgtcaaag gcgtgctgcg
    39181 tattcccgaa gggctggtac gaatcaaccg tcagggcgat gacctgcaca ttgaaacgca
    39241 aaacgttgcg ccaccggaca gccgtattga gctgatttcc agcagcgaag ctgactggaa
    39301 tgctttgcag agcgcgctgt tgaagcttcg tttagcgact accgcgtaag gttgcctgcg
    39361 tttttcagta agataattag agaaaatatg attaaaaatt tgccgcaaat agtgttgttg
    39421 aatattgtcg gcctcgcgct gtttctttcc tggtatattc ccgttaatca tggattctgg
    39481 ttgccgattg atgcggatat tttttatttc tttaatcaga aactggtcga aagtaaggcc
    39541 tttttgtggc tggttgcatt gaccaacaat cgcgctttcg acggttgttc tctgctggcg
    39601 atgggaatgt tgatgctgag tttctggctg aaagaaaacg cccctggcag acgacgtatc
    39661 gtgattatgg gtctggtcat gctattaact gcagtggtat taaaccagct gggtcaggca
    39721 ttaattcctg taaaacgggc cagcccaaca ttgactttta ccgatattaa ccgcgtcagc
    39781 gaactgctct ctgttcccac gaaagatgcc tcacgagata gctttcccgg cgatcacggc
    39841 atgatgctgc ttattttttc ggcattcatg tggcgttatt tcggcaaagt tgcaggcctt
    39901 atcgccctta ttatttttgt ggtttttgca tttcccagag taatgattgg cgcacactgg
    39961 tttactgaca tcattgtcgg ttcgatgacc gtgatattga tcggtttgcc ctgggtgttg
    40021 ctgacgccat taagtgatcg attaatcacc ttttttgaca aatcactacc gggaaaaaac
    40081 aaacatttcc aaaacaaata actcacagta attaacatca tcaggcttat ttttatagtg
    40141 aggataatcc tgatgatgcg caccgtgctt tcatccatcg cgcgcaaaaa tcattctcta
    40201 agtaaatgaa tggattgcat gcgtttcact caatcgtact ttaattgacc aatcccgctt
    40261 attaactttc tgtatcactt tttcttataa aaaatcatgt aaaaccgctc gccaagaccg
    40321 caccaatcgg gtaatctcga actcgttttg cctcggcggt agattatcct cacagcatat
    40381 aattttgtgc gttagtccac agatttggcc ttaaggaatt gtttcaacat gcccaggtaa
    40441 ttagtctcgt gccgcttggc atttttttat aacgatattt gtcgttaagg acttcaaggg
    40501 aaaacaaaca acatggtcaa atctcaaccg attttgagat atatcttgcg cgggatcccc
    40561 gcgattgcag tagcggttct gctttctgca tgtagtgcaa ataacaccgc aaagaatatg
    40621 catcctgaga cacgtgcagt gggtagtgaa acatcatcac tgcaagcttc tcaggatgaa
    40681 tttgaaaacc tggttcgtaa tgtcgacgta aaatcgcgaa ttatggatca gtatgctgac
    40741 tggaaaggcg tacgttatcg tctgggcggc agcactaaaa aaggtatcga ttgttctggt
    40801 tttgtacaac gtacattccg tgagcaattt ggcttagaac ttccgcgttc gacttacgaa
    40861 cagcaggaaa tgggtaaatc tgtttcccgc agtaatttgc gtacgggtga tttagttctg
    40921 ttccgtgccg gttcaacggg acgccatgtc ggtatttata tcggcaacaa tcagtttgtc
    40981 catgcttcca ccagcagtgg tgttattatt tccagcatga atgaaccgta ctggaagaag
    41041 cgttacaacg aagcacgccg ggttctcagc cgcagctaat aaaccgtttg gatgcaatcc
    41101 cttggctatc ctgacgagtt aactgaaagc actgcttagg cagtgctttt ttgttttcat
    41161 tcatcagaga aaatgatgtt tccgcgtctt gatccaggct atagtccggt cattgttatc
    41221 ttttaaatgt tgtcgtaatt tcaggaaatt aacggaatca tgttcatacg cgctcccaat
    41281 tctggacgta agctcctgct tacctgcatt gtagcaggcg tgatgattgc gatactggtt
    41341 agctgccttc agtttttagt ggcctggcat aagcacgaag tcaaatacga cacactgatt
    41401 atcgacgtac aaaagtatct cgatacctat tttgccgacc tgaaatccac tactgaccgg
    41461 cttcagccgc tgaccttaga tacctgccag caggctaacc ccgaactgac cgctcgcgcg
    41521 gcgtttagca tgaatgtccg tacgtttgtg ctggtgaaag ataaaaaaac attctgttca
    41581 tctgcgactg gtgagatgga cattccactc aatgaattga ttccggcgct cgacattaat
    41641 aaaaacgtcg atatggcgat cttacccggc acgccgatgg tgccgaacaa acccgcaatc
    41701 gtcatctggt atcgcaaccc tttgctgaaa aatagcggtg tgtttgccgc tctgaatctc
    41761 aacctgacgc cttctctctt ttatagttca cggcaggaag attacgatgg cgtcgccctc
    41821 attattggca atactgcgct atctaccttt tcttcacgtt tgatgaacgt taatgaatta
    41881 accgacatgc cggtccgtga aactaaaatt gcgggcattc ctctgaccgt tcggctttat
    41941 gcggatgact ggacatggaa cgatgtgtgg tacgcatttt tactgggcgg catgagtgga
    42001 actgtcgttg gcctgctctg ctattacctg atgagcgtac gtatgcgccc cggcagagaa
    42061 atcatgaccg ccatcaagcg cgaacaattt tacgtggcgt atcaaccggt ggtggataca
    42121 caagctttgc gagtaacggg cctggaagta ctgctacgct ggcggcatcc tgtcgcggga
    42181 gaaattcccc cggatgcctt cattaacttt gccgaatcgc aaaagatgat tgtgccgctg
    42241 actcagcacc tgtttgagtt aattgcccgc gatgccgcag aattagaaaa agtgctgcca
    42301 gtaggcgtca aatttggtat taacattgcg ccggaccatc tgcacagcga aagctttaaa
    42361 gcagatatcc agaaactgct cacttccctg cccgcacacc atttccagat tgtgctggaa
    42421 attaccgagc gcgatatgct gaaagagcaa gaagccacac aactcttcgc ctggctgcac
    42481 tcggtcggcg tagaaattgc tattgatgac ttcggcaccg ggcacagcgc gcttatctat
    42541 cttgagcgtt ttacgctcga ttatctgaaa attgaccgtg gatttatcaa cgccatcggt
    42601 acggaaacga tcacttcacc cgtacttgac gcggtgctga cgctggcgaa acgcctcaat
    42661 atgctgacgg ttgctgaagg ggtcgaaacg ccggaacagg cgcgatggct aagcgaacgc
    42721 ggcgttaatt tcatgcaagg ctactggatt agtcgccctt taccgctgga cgattttgtc
    42781 cgctggctga agaaaccgta tacgccgcag tggtaaggtg tgcttacgtc ccttattatt
    42841 catagtgaaa gcatgccgga ttgcggctaa tgatgagtaa aaggaaatcc attgcagatg
    42901 attgtgcgca tactgctgct gtttatcgct ctgttcacct ttggtgtgca ggcgcaggct
    42961 atcaaggaaa gctatgcctt tgccgtgctg ggcgaacccc ggtacgcgtt taatttcaac
    43021 cattttgatt atgtgaaccc cgccgcgcca aaaggtgggc agataacgtt gtcagccctc
    43081 ggcaccttcg ataatttcaa ccgctatgca ctgcgcggca acccgggcgc acgcaccgag
    43141 cagctgtacg acacgctatt tacgacttcc gatgacgaac caggcagtta ttacccgctg
    43201 attgctgaaa gcgcacgcta tgctgacgat tattcctggg tggaggtcgc tattaatccg
    43261 cgcgcccgtt ttcatgatgg ttcgcccatt actgcccgcg atgtagagtt tacttttcaa
    43321 aaatttatga ccgaaggcgt gccgcaattt cgtctggtct acaaaggcac caccgtcaaa
    43381 gccattgcac cgttaaccgt gcgcattgag ttagctaaac ccagcaaaga agatatgctg
    43441 agtctgtttt cgctgccgat atttccagaa aagtactgga aggatcacaa acttagcgac
    43501 ccgctcgcca cgcctccgct tgccagtggt ccgtaccgcg ttacgtcctg gaaaatgggg
    43561 caaaatattg tctattcccg cgtgaaagat tactgggcag caaacttacc ggtaaaccgt
    43621 ggacgctgga atttcgacac cattcgctac gattattacc tcgatgataa tgtcgccttt
    43681 gaagcgttta aagcaggtgc ctttgatttg cgtatggaaa acgacgctaa aaactgggcc
    43741 acgcgttata ccggtaaaaa tttcgataaa aaatacatca tcaaagatga gcaaaagaac
    43801 gaatcagccc aggatacgcg ctggctggcg tttaatatcc aacgtccggt attcagcgat
    43861 cgccgggtcc gggaagcaat cactctcgcc tttgactttg aatggatgaa caaggcgttg
    43921 ttttacaacg cctggagtcg cacgaacagt tattttcaga ataccgaata cgcggccaga
    43981 aattaccccg acgccgcgga gctggtgctt ctggcaccaa tgaaaaaaga tctaccgcca
    44041 gaagtcttca cacaaatcta ccagccgccg gtatctaaag gcgatggcta cgatcgtgac
    44101 aacctgttaa aagccgacaa acttctcaac gaaacgggct gggtgctgaa gggtcagcaa
    44161 cgcgttaatg ccacaacggg tcagccgctc agctttgaat tattgcttcc cgcaagcagc
    44221 aatagtcagt gggtattgcc gttccagcac agcctgcaac ggctgggtat caatatggac
    44281 attcgcaagg tggataactc gcaaatcact aaccgcatgc gcagtcgcga ctatgacatg
    44341 atgccgcgcc tatggcgggc gatgccgtgg cccagttccg atttacagat ttcctggtca
    44401 tcggaatata tcaattccac ttataatgcc cccggcgtgc aaagcccggt tatcgactcg
    44461 ctgatcaacc aaattattgc cgcgcaggga aataaagaaa aattactgcc gttggggcga
    44521 gcactggatc gcgtattaac gtggaattat tacatgctgc caatgtggta catggcggaa
    44581 gaccgtctcg cctggtggga taaattctcc caacccgctg tacgccctgt ttacagcctg
    44641 ggtatcgata cctggtggta tgacgttaat aaagcggcca aactgccgtc agccagacaa
    44701 cagggagagt agatgggcgc ttacctgatt cgccgtctgt tgctggtgat cccaacgtta
    44761 tgggctatta tcactatcaa ctttttcatc gtgcaaattg cgcctggcgg tccggtcgat
    44821 caggccatcg ccgccattga gtttggtaat gccggagtat tacccggcgc aggcggtgaa
    44881 ggtgttcgtg ccagccatgc gcaaacgggt gtcggcaata tcagcgacag taattaccgt
    44941 ggcggacgcg gattagaccc agaagtgatc gctgagatca ctcatcgcta cggtttcgat
    45001 aagccgatcc acgaacgtta cttcaaaatg ctctgggact acatccgctt tgattttggt
    45061 gatagcctgt ttcgcagcgc ctcggtgctg acgctgatta aagacagtct gccggtttcc
    45121 atcaccctcg gattgtggag cacgctgatt atctatctgg tgtcgattcc gttaggcatt
    45181 cgcaaagctg tttataatgg gagtcgcttt gacgtctgga gtagcgcatt tatcatcatc
    45241 ggctacgcca ttccggcctt tttgtttgcc atcctgctga ttgtcttctt cgcgggcggc
    45301 agctatttcg acctgttccc tctacgcggc ctggtttccg ctaactttga ttcgctgccg
    45361 tggtatcaga aaatcaccga ttatctgtgg catatcacgc taccggtgct ggcgacagtg
    45421 attggtggct ttgcggcgct gaccatgctg accaaaaact cattccttga tgaagtgcgc
    45481 aagcaatacg tggtgaccgc gcgtgcgaaa ggggtaagtg agaaaaacat tctctggaaa
    45541 catgtgttcc gcaacgccat gctgctggtg attgccggtt ttccggcgac gtttatcagc
    45601 atgtttttta ccggctcgct gctgattgag gtgatgtttt cactcaatgg tcttggctta
    45661 ctgggctacg aagcgaccgt ctcgcgcgat tatcctgtaa tgtttggtac cttgtatatt
    45721 ttcaccctga ttggcctgct gctgaatatt gtcagtgata tcagctatac gctggttgat
    45781 ccgcgtatag attttgaggg acgttaatgt cgcgactcag ccccgtcaat caggcccgtt
    45841 gggcgcgttt tcgtcataac cgtcgcggct actggtcgtt atggattttc ctcgttttgt
    45901 ttggtttgag tttgtgttct gaacttatcg ctaacgataa accgttgctg gtgcgttatg
    45961 acggcagttg gtatttcccg ttgttgaaaa actacagcga aagcgatttt ggcggcccgc
    46021 tggcaagtca ggctgattat caggacccgt ggctgaaaca acggctggaa aataacggct
    46081 gggtactgtg ggcaccgatt cgctttggtg ctaccagtat caactttgct accgataagc
    46141 ccttcccttc tccaccctcc cggcaaaact ggctgggaac ggatgccaac ggcggcgatg
    46201 tgctggcacg cattctctat ggcacgcgga tctcggttct gtttggcctg atgctgactc
    46261 tctgttccag cgtgatgggc gtgctggcgg gggcgctaca aggctattac ggcggtaaag
    46321 tcgatctctg gggacaacgc tttattgaag tatggtcggg gatgccgacg ctgtttttga
    46381 ttattttact ttccagcgtc gtacagccta acttctggtg gctgctggca attactgtct
    46441 tgtttggctg gatgagtctg gtcggcgtgg tgcgggcgga gtttttacgt actcgtaatt
    46501 tcgactacat tcgtgcggca caggcgcttg gcgtcagcga tcgcagtatc atcctgcgtc
    46561 atatgttgcc taatgccatg gtcgcgaccc tcaccttttt accgtttatt ttatgtagtt
    46621 cgataaccac cctgacctcg ctcgatttcc tcggcttcgg tctaccgctc ggttcaccgt
    46681 cactcggtga actgctgtta caagggaaaa ataaccttca ggccccgtgg cttgggatca
    46741 ccgccttctt gtcggtggcg atattattgt ctttgctgat ctttattggt gaagccgtcc
    46801 gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc aaactctgtt agcgattgaa
    46861 aatttgtcgg tgggttttcg ccatcagcaa accgtacgta cagtagtcaa tgatgtttca
    46921 ctacagattg aggctggcga aacgctggcg ctggtgggtg agtcaggttc aggcaaaagc
    46981 gttaccgcgc tgtcaatttt acgcctgctc ccttccccgc cggttgaata tctctccggc
    47041 gatattcgtt ttcatggcga atcgctgctt catgccagcg atcaaacgtt gcgcggtgta
    47101 cgcggtaata agatcgccat gatttttcag gaaccgatgg tgtcgttaaa tccattgcat
    47161 accctggaaa aacagcttta tgaagtgctt tcactccacc gcgggatgcg tcgggaagcg
    47221 gctcgtggcg aaattcttaa ctgccttgat cgcgtcggta tccgccaggc ggcaaaacgg
    47281 ctgacagatt atccgcatca gctctccggc ggcgaacgcc agcgggtgat gattgcgatg
    47341 gcgctgttaa cgcgaccgga attattaatt gccgatgaac cgaccaccgc actggacgtc
    47401 tctgtccagg cgcagatttt acagctgttg cgcgaactgc aaggcgagct gaatatgggc
    47461 atgctgttta ttactcataa cctcagcatt gtcagaaaac tggcccaccg cgtggcggta
    47521 atgcaaaacg gtcgctgtgt cgagcaaaat aacgccgcta cgctatttgc ctcacccact
    47581 catccttaca cacaaaagct actcaacagt gaaccgtcag gcgatccggt accgttgcca
    47641 gaacctgcct caacgttgct ggatgttgaa cagcttcagg ttgccttccc cattcgcaaa
    47701 gggattttga agcgcattgt ggatcataat gtggtggtga aaaacatcag ttttacgcta
    47761 cgggcgggtg aaacactggg tttagtgggc gagtctggtt ccgggaaaag cacgacggga
    47821 ctggcgctgc tgcgactgat tacctctcag ggcagcatcg tctttgacgg tcagccactg
    47881 caagatttaa atcgccgcca gctgttacct actcgtcatc gtattcaggt ggtatttcag
    47941 gatccaaact cctcgctcaa cccacgactc aacgttttgc agattattga ggaaggctta
    48001 cgggttcacc agccgacgct ttctgccgca caacgcgaac aacaagtgat agccgtgatg
    48061 catgaagtgg gattagatcc tgaaacacgc caccgttatc cggcggagtt ctctggtggt
    48121 cagcgacaac gtattgcgat tgccagggca ttaattctta agccctcact gatcatactt
    48181 gatgaaccga catcatcact cgacaaaacg gtacaggcgc aaatattgac gctattgaaa
    48241 tcattgcaac aaaagcatca actggcctat ttgtttatca gccacgattt gcacgttgtc
    48301 cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg aagtagtgga acaaggaccg
    48361 tgcgcgcgcg tgtttgccgc accgcagcag gagtatacgc gtcagctact ggcgttgagc
    48421 tgacgcttaa aaaggattgt agtttgaaaa gggttcggca attgccacac caaaattttt
    48481 caatcggcat gttgcagcaa actcgtcctg gcgatttaca aacaggcacg gctcaccttc
    48541 acactccagc accgaacatg gaacttcgat gtcgcttaac gcctggctta gtttatacat
    48601 caccgaccat gcattatcac catccgggct aagcaattta agcgccacca ggctgttgtc
    48661 accgcacggt ccattttgcg gaatcggttc aaccttcgaa cctgcgaaac cagcagacca
    48721 gcgaaagttc tgcggtagac gatggacgat tgagagttgt aatgaagtca cttattttcc
    48781 ccggaagcac atttacttca caatttattt acatcaattt taacacatca tcaacaatcc
    48841 gtctttatac agatcgtaaa gattgatgcc tcgcctgcat ggcctctatg gctctgattt
    48901 aagtaatatc tgcgggcgtg gtcacgtttg cggggctata tgtacccgtt tctgcgatct
    48961 aactcaacct ttttaactac aatgatgtga ctttttacat aaattgattt tacataaaat
    49021 aaacatatat cgggggaatg atagatttgt ggttgacatg catcaacaaa ggaagccttt
    49081 tagcttcctc gttgtgcaat agatcaccgt tttttcggtc gactggcgta cagacagaag
    49141 agaatggagc tggttgcgca gaatgcaatt gaccaaatca tcggccatgc agagttaaag
    49201 gtcgcaagag aaagcaatgc gccaacaatt gcccctatgc caaaacggaa ggttcctgcc
    49261 agcgaagatg ccgttcccgc catatgggga aactcatcaa gaatgaccgc catcgcattg
    49321 gatgacacca tagacacgca gcccacaaac gccgcaacgc caaccaccag cgaccagaat
    49381 cccagcccca gcagcgcact gatgaccatc cacgctgcca taataaattg tatccacaac
    49441 cccgagcgga acatatttaa cgcgccaatg cggcggacga agcggctgtt aaagatggtc
    49501 atcacgaaca gaaaaacaat gtttagcgca aagtaataac caaagttttc cggtgcaacg
    49561 tggttgattt caatgtagac aaacggtccg gcgctcagga acgagaacat tccggcaaag
    49621 ctgaaaccgc tggccagcat ataactcagg acacgcttat gacggaacag cgccgcaaag
    49681 ttaccaatag tggtacgaat gtgaaaaggc tggcgaggat ccagaagatg taatgccagc
    49741 tcagccacac cagcacccag ccgccaacta tcggtgccat cagcggtgca atggtcgtca
    49801 ccagcatgac aaacgacatc atccgcgaga actcttcttt cgggtaaata tcacgcatca
    49861 aagcgttgat aaccacgctg gccgcagccg cagccagccc gtggaagaaa cgcatcacaa
    49921 tcagctgatc gatggtttgt gccaacgcac acgccaccgc ggcggcggca aacaccagcg
    49981 taccgccaag aaccaccggc ttacgcccga agctgtctgc catcggcccg tagattaact
    50041 gccccaacgc aaagcccaga atataagtac tcagggtcat ctgcgtactg cccgccggta
    50101 cgccaaactg cgctgaaatt accggtagcg cgggcagata catatcaatc gacagcggca
    50161 tcaacatggc cagcaggcca aggataaaaa caatagcaaa cgacgaatgc tgtcgggtgg
    50221 tcacaacggg ctcctgaaag tcattgaaaa gttagacgac gctggcaatt tcttcttcag
    50281 ttaacggacg atattcaccg ggggctaaat cagcatccag cgtaatgccg ccaatacgtt
    50341 cacgatgcag ctcaaccaca tggttaccca cggcggcgaa catgcgtttc acctgatgat
    50401 aacgcccttc gctgatggtc agacgaacct gcgttggggt aatcacttcc agcaccgccg
    50461 gcttagtgag atctttttcg ttatgcagct gcacgccttt agcaaattgt tctgccgtat
    50521 cgtcagctac gggtgatacc agcgtcacca gataggtctt ctcgcaatga tggcgcggag
    50581 aagtaatgcg gtgcgaccac tgaccatcat ctgtcattag taccagaccg gtggtatcga
    50641 tatccagccg ccctgcggcg tgcagtttcc acgctaccgg ttcatctaga aaatagagca
    50701 ccgttgggtg atcagggtcg tccgtggagc aaacataacc ctgaggctta ttgagcatga
    50761 agtaacgtgg accgtgttgc tgcgccagcg ggttgccatc gtaagcgaca tcatgttcag
    50821 gaagcagttt gaacgctgca ttacggacga tttcgccatc gacggtgaca cgattgccgc
    50881 ggatttcacg cccggcaata gcacggctaa cgccgagttg ctgtgcgata aatttatcaa
    50941 gtcgcatgtg tgtgattttg cctgtaaaaa acggaggtcg ggtaatgacc cgaatatctg
    51001 aactgttgtc tgcccagtat agcggtctaa taacatccct caagggaaaa agatccgtgg
    51061 catactatta gcagaataat ctacctacgc gagaccatga tttttacact tcgcccatat
    51121 cagcaagaag ccgtggatgc cacgctcaac cattttcgtc gtcataaaac ccctgccgtt
    51181 atcgtgctgc ccaccggtgc aggtaaaagc ctggtgatag cggaactggc acggctggct
    51241 cgtggtcgcg tactggtgct ggcacacgtt aaagaactgg tggcgcaaaa ccatgcaaag
    51301 tatcaggcgc tggggctgga agccgatatt tttgccgccg ggctgaagcg taaagagagc
    51361 cacggtaaag tggtatttgg cagtgtgcag tcggtcgccc gtaatattga tgcctttcag
    51421 ggtgaatttt cgctgttgat tgtcgatgaa tgtcaccgta ttggtgacga tgaagagagc
    51481 cagtatcagc aaatcctcac tcacctgaca aaagtgaatc cccacttacg cctgctgggg
    51541 ctgactgcca cgccttttcg attgggcaaa ggttggatct accagttcca ttatcacggc
    51601 atggtacgca gcgatgagaa agcccttttc cgtgactgca tttatgagct gcccctgcgt
    51661 tatatgatta aacacagcta tctgacgccg ccagaacgac tggatatgcc agtagtgcaa
    51721 tatgatttca gccgcttgca ggcacagagc aacgggctgt tcagcgaagc cgatctcaac
    51781 cgtgagctga aaaaacagca acgtattacc ccgcacatca tcagccagat tatggagttc
    51841 gccgaaaagc gtaaaggagt gatgattttc gccgcgacgg tcgaacacgc aaaagagatt
    51901 gtgggattac tgcctgccga agatgcagca ctgattactg gcgacacccc cggcgctgag
    51961 cgcgatgtgt taattgaaaa ttttaaagcc cagcgttttc gctatctggt caacgtcgcg
    52021 gtactgacca ccggatttga cgccccgcac gtcgatctta tcgccattct gcgccctacc
    52081 gaatcagtga gtctttacca acaaattgtc gggcgcggtc tgcgtctcgc tccgggcaag
    52141 actgattgtt taattcttga ttatgcgggt aatcctcacg atctctacgc gccggaagtt
    52201 ggtacaccga aaggcaaaag tgacaacgtt ccggtacagg ttttctgccc tgcctgcggt
    52261 tttgccaaca ccttttgggg gaaaacgacc gccgacggga cattgattga acactttggt
    52321 cgccgctgtc agggatggtt tgaagatgac gacggtcatc gcgaacagtg tgacttccgt
    52381 ttccgtttta aaaattgccc gcaatgtaac gcagaaaatg atattgccgc tcgccgctgc
    52441 cgcgaatgtg acacggtact ggttgatccg gatgatatgt taaaagcggc gctacgactg
    52501 aaagatgcgc tggtattacg ctgtagcggc atgtctttgc agcatgggca cgacgagaaa
    52561 ggcgaatggt tgaaaatcac ctattacgat gaagacggtg cggatgtgag tgagcgtttc
    52621 cgtctgcaaa cacctgccca gcgtaccgcc ttcgagcagc tttttatccg cccgcatacg
    52681 cgcacaccgg gcatcccgct gcgctggatc accgccgccg atatcctcgc ccagcaagcc
    52741 ttattgcgac ccccggattt tgtcgtcgcc cgcatgaaag gccagtactg gcaggtgcgt
    52801 gaaaaagtgt tcgattacga aggtcgtttt cgtcgggcgc acgaattacg cggttaatgg
    52861 tagttttgat tgatgtataa ggcgattgag tatagaatct cacccgcttt tgcatacgca
    52921 aagcagatca cttacctgtt gctgggtcgc ctgtagcagg attaatttaa gagagaaaga
    52981 aatgtttact atcaacgcag aagtacgtaa agagcagggt aagggtgcga gccgccgcct
    53041 gcgtgccgct aacaagttcc cggcaatcat ctacggtggc aaagaagcgc cgctggctat
    53101 cgagctggat cacgacaaag tcatgaacat gcaagctaaa gctgaattct acagcgaagt
    53161 tctgaccatc gttgttgacg gtaaagaaat caaagttaaa gctcaggacg tacagcgtca
    53221 cccgtacaaa ccgaagctgc agcacatcga cttcgttcgc gcttaattgc tgaataagtt
    53281 gtaaaaaacc ccgctccggc ggggtttttt gtatctgcag attatgcctg attacggtat
    53341 tgctattttt tgcaggccag ataaggcgtt cacgccgcat ctggcattca gaacacaaaa
    53401 ccgattaatt gccgccagac gtccggcgct gcaattggtc gcgcaaattc ggcggcgtgc
    53461 ctttaatggt cagggtatcg gtcgccggct cccagaagat gcgttcacct aacagcatcg
    53521 catcaaagtt aatagtcaag ccaccgccgc taccggcaaa cttcgtcaac tgacgcagtg
    53581 tgctccggtc tgccgggaag ctctcttcca gttcgtagcc tttttcagcc gcaaactccg
    53641 tgaagctcac ttcgcttacg cctgccagct ctttcgacag cgattccagt tcgatctctt
    53701 cgcctgcctg cagctgctca ttacagtagc tataaacctg ctggcgcacg ttctgtcgct
    53761 ctgctttatc cagttgtgct tcggcagtga aatcatcaac cgcttgcaac agcccacggt
    53821 tttgcgcttt cgtgttcaat ccttcgctgg ccccgaggaa atccataaag aaatccgcca
    53881 ctttgcgccc tactcgccct ttcaggaaag tgagatagcg ggtggactct ggattggttt
    53941 cccattcggt taaatcgata cgcgcaacaa tatccgcatg attgatatca agataatggg
    54001 ttgggttgat gtcgagattt tcgttaacac gcatactgct caggttgctc agaactgcca
    54061 ccagcagata ctccaccgcc agataacggt agtggcaaaa cagcacgaat ccaccgtcag
    54121 caaaaggata tttcgctaat tcgtcacgca ggcgaccagt tgccgcgcgg ctaaatgcca
    54181 gaaagtcctc ttcgccctga cgctgtaagc gcagcgtctg cgccagttca ctctcttcgc
    54241 taaacaagcc gtaagcttta tttttggcgc tatagacccg atgcagttct gccaccatct
    54301 caacgacggt ttctgtcggt tccagcaatg aatcgcgcaa caccagctca aggttctgct
    54361 catcacgctt gataagctgg tgcagagcaa tctggttgat atccagactc atgataaact
    54421 ctcctttaag accgggcggt attcaaccac cgccggttaa gcgacgcaat aaaagatggc
    54481 ggtcatttta cagaaggcag cgtaacgaga atgatttaac gcataaaaaa gcagaaaaaa
    54541 aaccgttgct acggtaatat gttgcccttt catcaactaa ctgatttcga tttatgccac
    54601 aaatttcccg ctacagtgat gaacaggttg aacaattgct cgcagagctg ctcaacgtac
    54661 tggaaaaaca taaggctccg accgaccttt ccctgatggt gttaggcaac atggtcacta
    54721 accttatcaa caccagcatt gccccggccc aacgccaggc aattgccaac tcttttgccc
    54781 gcgccttaca gtcctctatc aacgaagaca aagcgcacta agggaaacag ataacaggtt
    54841 atggtaactc atcgtcagcg ctaccgtgaa aaagtctccc agatggtcag ttgggggcac
    54901 tggtttgcac tgttcaatat tctgctttcg ctcgtcattg gcagccgtta cctgtttatc
    54961 gccgactggc cgacaacgct tgctggtcgc atttattcct acgtaagcat tatcggccat
    55021 ttcagcttcc tggtgttcgc cacctacttg ctgatcctct tcccgctgac ctttatcgtc
    55081 ggctcccaga ggctgatgag gtttttgtcc gtcattctgg caacggcggg aatgacgcta
    55141 ttactgatcg atagcgaagt ctttactcgt ttccatctcc atcttaatcc catcgtctgg
    55201 cagttggtta tcaacccaga cgaaaatgag atggcgcgcg actggcagct gatgttcatc
    55261 agcgtgccgg ttattttatt gcttgaactg gtgtttgcga cgtggagctg gcaaaagctg
    55321 cgcagcctga cgcgtcgtcg acgcttcgcg cgcccgctgg ccgcattctt atttatcgcc
    55381 tttatcgcct cgcatgtggt gtatatctgg gccgatgcca acttctatcg cccgatcacc
    55441 atgcagcgcg ctaacctgcc gctttcgtac ccgatgacgg cgcgacgttt tcttgagaag
    55501 catggtctgc ttgatgcgca ggagtatcaa cgccgtctta ttgagcaagg taatccagac
    55561 gccgtttccg ttcagtatcc gttaagcgaa ctgcgctatc gcgatatggg caccgggcag
    55621 aatgtgctgt tgattactgt cgatggcctg aactactcac gcttcgagaa gcagatgcct
    55681 gcgctggcag gttttgctga gcaaaatatt tcgttcacgc gccatatgag ctccggcaac
    55741 actacagaca acggcatctt tggcctgttc tatggtatct cgccgagcta tatggacggc
    55801 attctgtcga cccgtacgcc tgcggcatta attactgcgc ttaatcagca aggctatcag
    55861 ctggggttat tctcatcaga tggctttacc agcccgctgt atcgccaggc attgttgtca
    55921 gatttctcga tgccgagcgt acgcacccaa tccgacgagc agaccgccac gcagtggatc
    55981 aactggctgg gccactacgc acaagaagat aaccgctggt tctcgtgggt ttctttcaat
    56041 ggcactaaca ttgacgacag caatcagcag gcatttgcac ggaaatatag ccgggcggca
    56101 ggcaatgtcg atgaccagat caaccgcgtg ctcaatgcac tgcgtgattc tggcaaactg
    56161 gacaatacgg ttgtgattat cactgccggt cggggtattc cgctgagcga agacgaagaa
    56221 acctttgact ggtcccacgg tcatctgcag gtgccattag tgattcactg gccaggcacg
    56281 ccggcgcagc gtattaatgc gctgactgat cataccgatc tgatgacgac gctgatgcaa
    56341 cgcctgctac atgtcagcac acctgccagc gaatattcgc aaggtcagga tttgttcaac
    56401 cctcaacgcc gtcattactg ggttactgca gcggataacg atacgctggc aattaccacc
    56461 ccgaaaaaga cgctggtgct gaacaataac ggtaaatacc gcacttacaa cttacgtggt
    56521 gaaagagtga aagatgaaaa accacagtta agtttgttat tgcaagtgct gacagacgag
    56581 aagcgtttta tcgctaactg attaattata aatcagttag cgaaatatct tacttgcaat
    56641 cggtgtggaa aacggtagta ttagcagcca cgagtcggca cgtagcgcag cctggtagcg
    56701 caccgtcatg gggtgtcggg ggtcggaggt tcaaatcctc tcgtgccgac caaaaatccc
    56761 aagaaaaaac caacctttac ggttggtttt tttatatctg caattaattt gataaacaga
    56821 ccgtgacaca tcacagcctg tttattttct gttatcagaa cgtccagacc actcccgcct
    56881 gagtattcca cggcgattct acccctgcgc cattgccata gctgactgac aaatgaccgc
    56941 ttaacgtcgg ggtaaacgat gaccttatcc cagcctgata aacgccacgg gttccagcca
    57001 catcattatt aaatttgcca tcgtcattaa ctttcacatg gttagaatcg gcctattcct
    57061 gacgcacggc ggctttcagc cagggttcca acgtcgtacc gttttgcagg tccatgtgat
    57121 agcttaccgc cgttcccgct tcagcgcgta atatccgggt atttcccaca tccgcgcgca
    57181 tgccgtttga taacgtgtag tcctgaccat ctgtggtaaa gccggtaaag gcgagatagg
    57241 ggctaacact ccacaatccg tcaacccaac ggaacccgct ctcaacatga gcacccgcgc
    57301 cgttactatt gtaatcgcca aacgctgttg ccccattact catcttgcca tggatggtgt
    57361 tggcaaaacg gtcaactttc accaccccat caacataggc accgttctga tgctcccaac
    57421 cggcataagc ccccagggtg tagctatcaa tattgccttt gccgccgcga tcaaaaccaa
    57481 tatcagaatg agagtaatca aagaacaagc cgcgaattgt gctgctttct tcacgggaga
    57541 aacggctatc gataccgagc gtcaggcccg tcaatgtttg ctcaaagcca gctcccgcat
    57601 cagtggtcac gttgttgcgg gtgttaattg ccgaactcca catcgccgta tcgtaattaa
    57661 cgccttttac gctaccaaga cgctcacgca cggtgtccag ttctgcatca aataccagcg
    57721 gttgtgctgc tgccatgttc agcacatcag tggttgaagg ggtaatttgc gcgcgattct
    57781 ctgcgaggct ccagctatgg ttgccattat ccagcaaggt atattcatac gtaccgatat
    57841 caacaacgcc tccggcattg cccaacgtga atgcagcatc accgccgccc gtcgttacca
    57901 gtgtaaggct atctcctgct gccgggctgg cccccgtgtc cgtcacgaat attttgaaat
    57961 cacctgttgc ctgaccgatg acgttgagct gatcgctctg atgattagcc atatcggtac
    58021 gcatataaaa ttgccgtttc ccgacagggt attggtggtc aacgtaatga aattttccgc
    58081 acttgccgtc acagatgaac gagtcactgg ctcagccatc ataatcacat ttgcgccgga
    58141 catcgcaagg ttggctaagt tgaggcgagt gttttggttc gtcgtagtga tatcggcaat
    58201 gatggtccat gcgctatttt cgagcgaaac gtccgcttgg ctggtatcca cggcaccatg
    58261 cgttctaaaa gaagcgccct cagaaaccac taccttaccc accaacatag cgggtgcata
    58321 atttattttc ggcttcatga cctcaagaat gccatcattc cctctgactg acacgttaac
    58381 cattgtgcca gcccagacat tagcgcggcc attgttgata accatatttt cagagatggc
    58441 tttagaggaa tacgtgatac ttccattctg atctgatcga ccgagatcat atacagcatt
    58501 tgcctgcact ttcgtaccag tatcctttcc cagcgattgc atagtggcat gctcatcaag
    58561 gatagtgtca atggcctgag tgtcctccat aacaatgagc ccggagccat catccagttc
    58621 ataatttttt gacacaccat cctttatact aaattggcct ttactgtttg taccactcac
    58681 ttccagcgca ttcgttgaga caattaattt cccgcctgct tttttatcaa cgccagttgc
    58741 agtcccgcca tccataacct ccagtaagcc gccactatcc acagtggtat tataagcgaa
    58801 gtcattttct tcaactcgca aacttccgcc gttttccaac agcatattgt tagcaatacc
    58861 attcttgatt tcgaactgac cgagacggtt tgttccgaat acctccatga cccgcgtggt
    58921 tgttttaata gcaccgcctg ctttctgatc taccgcaaat gctaatcccc cgtctttaac
    58981 ctccagtaat cctccctttt cgatagcggc aaagacggca atcccatctt tttcaacaat
    59041 taagcgcccg cctttaatcg tgggatcatt cgccgcg
//
LOCUS       DAZPBM010000005        58905 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPBM010000005 DAZPBM010000000
VERSION     DAZPBM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.005"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(119..1477)
                     /gene="plaP"
                     /locus_tag="ACHUZQ_000248"
     CDS             complement(119..1477)
                     /gene="plaP"
                     /locus_tag="ACHUZQ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1024056.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1467..1529)
                     /gene="yoeI"
                     /locus_tag="ACHUZQ_000249"
     CDS             complement(1467..1529)
                     /gene="yoeI"
                     /locus_tag="ACHUZQ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1024057.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1744..2673)
                     /locus_tag="ACHUZQ_000250"
     CDS             complement(1744..2673)
                     /locus_tag="ACHUZQ_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1024058.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2719..3543)
                     /locus_tag="ACHUZQ_000251"
     CDS             complement(2719..3543)
                     /locus_tag="ACHUZQ_000251"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1024059.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3626..3880)
                     /gene="yoeB"
                     /locus_tag="ACHUZQ_000252"
     CDS             complement(3626..3880)
                     /gene="yoeB"
                     /locus_tag="ACHUZQ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1024060.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3877..4128)
                     /gene="yefM"
                     /locus_tag="ACHUZQ_000253"
     CDS             complement(3877..4128)
                     /gene="yefM"
                     /locus_tag="ACHUZQ_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1024061.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4411..4461
                     /gene="hisL"
                     /locus_tag="ACHUZQ_000254"
     CDS             4411..4461
                     /gene="hisL"
                     /locus_tag="ACHUZQ_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR1024062.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4437..4560
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4607..5506
                     /gene="hisG"
                     /locus_tag="ACHUZQ_000255"
     CDS             4607..5506
                     /gene="hisG"
                     /locus_tag="ACHUZQ_000255"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1024063.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5512..6816
                     /gene="hisD"
                     /locus_tag="ACHUZQ_000256"
     CDS             5512..6816
                     /gene="hisD"
                     /locus_tag="ACHUZQ_000256"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1024064.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6813..7883
                     /gene="hisC"
                     /locus_tag="ACHUZQ_000257"
     CDS             6813..7883
                     /gene="hisC"
                     /locus_tag="ACHUZQ_000257"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1024065.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7883..8950
                     /gene="hisB"
                     /locus_tag="ACHUZQ_000258"
     CDS             7883..8950
                     /gene="hisB"
                     /locus_tag="ACHUZQ_000258"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1024066.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8950..9540
                     /gene="hisH"
                     /locus_tag="ACHUZQ_000259"
     CDS             8950..9540
                     /gene="hisH"
                     /locus_tag="ACHUZQ_000259"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1024067.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9540..10277
                     /gene="hisA"
                     /locus_tag="ACHUZQ_000260"
     CDS             9540..10277
                     /gene="hisA"
                     /locus_tag="ACHUZQ_000260"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1024068.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10259..11035
                     /gene="hisF"
                     /locus_tag="ACHUZQ_000261"
     CDS             10259..11035
                     /gene="hisF"
                     /locus_tag="ACHUZQ_000261"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1024069.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11029..11640
                     /gene="hisIE"
                     /locus_tag="ACHUZQ_000262"
     CDS             11029..11640
                     /gene="hisIE"
                     /locus_tag="ACHUZQ_000262"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1024070.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11737..12714)
                     /gene="wzzB"
                     /locus_tag="ACHUZQ_000263"
     CDS             complement(11737..12714)
                     /gene="wzzB"
                     /locus_tag="ACHUZQ_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1024071.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12860..14026)
                     /gene="ugd"
                     /locus_tag="ACHUZQ_000264"
     CDS             complement(12860..14026)
                     /gene="ugd"
                     /locus_tag="ACHUZQ_000264"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR1024072.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14276..15682)
                     /gene="gndA"
                     /locus_tag="ACHUZQ_000265"
     CDS             complement(14276..15682)
                     /gene="gndA"
                     /locus_tag="ACHUZQ_000265"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1024073.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15846..17216)
                     /gene="cpsG"
                     /locus_tag="ACHUZQ_000266"
                     /gene_synonym="manB"
     CDS             complement(15846..17216)
                     /gene="cpsG"
                     /locus_tag="ACHUZQ_000266"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1024074.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(17321..18757)
                     /gene="cpsB"
                     /locus_tag="ACHUZQ_000267"
     CDS             complement(17321..18757)
                     /gene="cpsB"
                     /locus_tag="ACHUZQ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1024075.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(18760..19983)
                     /gene="wcaI"
                     /locus_tag="ACHUZQ_000268"
     CDS             complement(18760..19983)
                     /gene="wcaI"
                     /locus_tag="ACHUZQ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1024076.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(19980..20459)
                     /gene="gmm"
                     /locus_tag="ACHUZQ_000269"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(19980..20459)
                     /gene="gmm"
                     /locus_tag="ACHUZQ_000269"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1024077.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(20462..21427)
                     /gene="fcl"
                     /locus_tag="ACHUZQ_000270"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(20462..21427)
                     /gene="fcl"
                     /locus_tag="ACHUZQ_000270"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1024078.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(21430..22551)
                     /gene="gmd"
                     /locus_tag="ACHUZQ_000271"
     CDS             complement(21430..22551)
                     /gene="gmd"
                     /locus_tag="ACHUZQ_000271"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1024079.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(22577..23125)
                     /gene="wcaF"
                     /locus_tag="ACHUZQ_000272"
     CDS             complement(22577..23125)
                     /gene="wcaF"
                     /locus_tag="ACHUZQ_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1024080.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(23141..23887)
                     /gene="wcaE"
                     /locus_tag="ACHUZQ_000273"
     CDS             complement(23141..23887)
                     /gene="wcaE"
                     /locus_tag="ACHUZQ_000273"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1024081.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(23898..25115)
                     /gene="wcaD"
                     /locus_tag="ACHUZQ_000274"
     CDS             complement(23898..25115)
                     /gene="wcaD"
                     /locus_tag="ACHUZQ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR1024082.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(25090..26307)
                     /gene="wcaC"
                     /locus_tag="ACHUZQ_000275"
     CDS             complement(25090..26307)
                     /gene="wcaC"
                     /locus_tag="ACHUZQ_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1024083.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(26304..26792)
                     /gene="wcaB"
                     /locus_tag="ACHUZQ_000276"
     CDS             complement(26304..26792)
                     /gene="wcaB"
                     /locus_tag="ACHUZQ_000276"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1024084.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(26795..27634)
                     /gene="wcaA"
                     /locus_tag="ACHUZQ_000277"
     CDS             complement(26795..27634)
                     /gene="wcaA"
                     /locus_tag="ACHUZQ_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR1024085.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(27727..29889)
                     /gene="wzc"
                     /locus_tag="ACHUZQ_000278"
     CDS             complement(27727..29889)
                     /gene="wzc"
                     /locus_tag="ACHUZQ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1024086.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(29892..30335)
                     /gene="wzb"
                     /locus_tag="ACHUZQ_000279"
     CDS             complement(29892..30335)
                     /gene="wzb"
                     /locus_tag="ACHUZQ_000279"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1024087.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(30341..31480)
                     /gene="wza"
                     /locus_tag="ACHUZQ_000280"
     CDS             complement(30341..31480)
                     /gene="wza"
                     /locus_tag="ACHUZQ_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1024088.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            31789..31938
                     /locus_tag="ACHUZQ_000281"
     CDS             31789..31938
                     /locus_tag="ACHUZQ_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024089.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            32139..33722
                     /locus_tag="ACHUZQ_000282"
     CDS             32139..33722
                     /locus_tag="ACHUZQ_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1024090.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(33996..35849)
                     /gene="asmA"
                     /locus_tag="ACHUZQ_000283"
     CDS             complement(33996..35849)
                     /gene="asmA"
                     /locus_tag="ACHUZQ_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1024091.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(35871..36452)
                     /gene="dcd"
                     /locus_tag="ACHUZQ_000284"
     CDS             complement(35871..36452)
                     /gene="dcd"
                     /locus_tag="ACHUZQ_000284"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1024092.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(36544..37185)
                     /gene="udk"
                     /locus_tag="ACHUZQ_000285"
     CDS             complement(36544..37185)
                     /gene="udk"
                     /locus_tag="ACHUZQ_000285"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1024093.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            37503..40820
                     /locus_tag="ACHUZQ_000286"
     CDS             37503..40820
                     /locus_tag="ACHUZQ_000286"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1024094.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(40930..41778)
                     /gene="alkA"
                     /locus_tag="ACHUZQ_000287"
     CDS             complement(40930..41778)
                     /gene="alkA"
                     /locus_tag="ACHUZQ_000287"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR1024095.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            41912..43264
                     /gene="yegD"
                     /locus_tag="ACHUZQ_000288"
     CDS             41912..43264
                     /gene="yegD"
                     /locus_tag="ACHUZQ_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1024096.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(43277..45217)
                     /gene="yegI"
                     /locus_tag="ACHUZQ_000289"
     CDS             complement(43277..45217)
                     /gene="yegI"
                     /locus_tag="ACHUZQ_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR1024097.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(45214..45975)
                     /gene="pphC"
                     /locus_tag="ACHUZQ_000290"
     CDS             complement(45214..45975)
                     /gene="pphC"
                     /locus_tag="ACHUZQ_000290"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR1024098.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIHQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(45972..46631)
                     /locus_tag="ACHUZQ_000291"
     CDS             complement(45972..46631)
                     /locus_tag="ACHUZQ_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1024099.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(46852..46911)
                     /gene="ibsA"
                     /locus_tag="ACHUZQ_000292"
     CDS             complement(46852..46911)
                     /gene="ibsA"
                     /locus_tag="ACHUZQ_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR1024100.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(47184..47240)
                     /locus_tag="ACHUZQ_000293"
     CDS             complement(47184..47240)
                     /locus_tag="ACHUZQ_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1024101.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(47511..47567)
                     /locus_tag="ACHUZQ_000294"
     CDS             complement(47511..47567)
                     /locus_tag="ACHUZQ_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1024102.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            47846..49087
                     /gene="mdtA"
                     /locus_tag="ACHUZQ_000295"
     CDS             47846..49087
                     /gene="mdtA"
                     /locus_tag="ACHUZQ_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR1024103.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            49087..52209
                     /gene="mdtB"
                     /locus_tag="ACHUZQ_000296"
     CDS             49087..52209
                     /gene="mdtB"
                     /locus_tag="ACHUZQ_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR1024104.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            52210..55287
                     /gene="mdtC"
                     /locus_tag="ACHUZQ_000297"
     CDS             52210..55287
                     /gene="mdtC"
                     /locus_tag="ACHUZQ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1024105.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            55288..56703
                     /locus_tag="ACHUZQ_000298"
     CDS             55288..56703
                     /locus_tag="ACHUZQ_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1024106.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            56700..58103
                     /gene="baeS"
                     /locus_tag="ACHUZQ_000299"
     CDS             56700..58103
                     /gene="baeS"
                     /locus_tag="ACHUZQ_000299"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1024107.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            58100..58822
                     /gene="baeR"
                     /locus_tag="ACHUZQ_000300"
     CDS             58100..58822
                     /gene="baeR"
                     /locus_tag="ACHUZQ_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1024108.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        13847 a        15673 c        15202 g        14183 t
ORIGIN      
        1 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaaa
       61 aataaaaaaa ccggagccaa atattcattc gactccggtt gattattaga aaagatgatt
      121 atgcaacgtc ttcgtactgc ggaactgggt tacggaagct cttggtgacg caagccaggt
      181 aaatcagacc gatagcagcc caaatcagac ccagaaccat tgagctttct tccaggttaa
      241 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
      301 ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
      361 cgaaggtgaa cgccaccaac gcaccaaagt taatcagcgc tgtagccatt accaggtcga
      421 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
      481 atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga cccattacgt
      541 acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg gtaatggtgg
      601 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
      661 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
      721 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
      781 cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag ccggtaaagg
      841 aaaagcacag gattgtcgcc ccggtaatca tcggaataac atgcgcatcg ccagaccaga
      901 acggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
      961 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgaagttcg
     1021 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     1081 acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     1141 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     1201 tcgggctaat ggatttctgg gcgtaagtat atgcagagcc agcagaagga tagcggcgaa
     1261 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     1321 tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac agcgtcatcg
     1381 gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     1441 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt tacggtgata
     1501 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     1561 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     1621 gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt tgacagcggc
     1681 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aagcttgtca taataatttt
     1741 tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     1801 tgctaaaatt tcatcccgcc accagccgct ggcaagccct gcggtctgtt cgttccagcc
     1861 aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     1921 atcgagaaaa cgttgcacca gataacgggg taaatagccg cagccgaggc cgctaatttg
     1981 cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     2041 tgaggccgta gaagcgcctg cctgcgcagt gtcccccacc acaatcgccc gatatcgctt
     2101 aatgatacga cggtttaacg gctcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     2161 aaagacttgt tccagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     2221 atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     2281 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     2341 cagtggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     2401 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     2461 ccgccccttc tctaataaca tttttccggt acgggtgaat ttagcgcggt gaccgctacg
     2521 atcaagtaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     2581 cggcgtcttg tagagtttag ccgacgccgc cgcaaaactg ccttcttttt ctaaggcatc
     2641 gaggatcatc aacacatcca gcagttgttt catacacgcc cccttgcagt gcatgcggcg
     2701 tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat actggtattc
     2761 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     2821 actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg gatagaaaac
     2881 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     2941 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     3001 accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgtcccg gtccgaccag
     3061 ccccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     3121 ttcttctaat actcgtccac tgttggtcac cggattacgc ggggtggttt ctttcaccgt
     3181 gccttgcgcg tcgccataga cagacgttga gctggtaaaa ataatgcgag gaatacgatg
     3241 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     3301 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccatcaggg catccagatc
     3361 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     3421 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     3481 tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg cgaccttttt
     3541 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     3601 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     3661 aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     3721 ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc gttctgcggg
     3781 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     3841 gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt ccttttctgt
     3901 tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt tagcggggga
     3961 gcgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca tcagaacaca
     4021 agcctctcca ttctgacgag taataaggat cggggcatga tcttcaacgg ctttcatcat
     4081 tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt caatctcctc
     4141 ttttgtacag tttattgtac aatgatgggc gttaattaac tatttattaa ttagtttgta
     4201 gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc tatttttggt gccgtcagct
     4261 tagaggacag tcctcttagc cccctccttt ccccgctcat tcattaaaca aatccattgc
     4321 cataaaatat ataaaaaagc ccttgctttc taacgtgaaa gtggtttagg ttaaaagaca
     4381 tcagttgaat aaacattcac agagactttt atgacacgcg ttcaatttaa acaccaccat
     4441 catcaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat tcagatcttc
     4501 cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg gcttttttat
     4561 tgcgcggttg ataacggttc agacaggttt aaagaggaat aacaaaatga cagacaacac
     4621 tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac gcgaattact
     4681 ggcgcgctgt ggcattaaaa ttaatcttca cacccagcgc ctgatcgcga tggcagaaaa
     4741 catgccgatt gatattctgc gcgtgcgtga cgacgacatt cccggtctgg taatggatgg
     4801 cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc ttaaccgccg
     4861 cgcccagggt gaagatccgc gctactttac cttgcgtcgt ctggatttcg gcggctgccg
     4921 cctttcgctg gcaacgccgg ttgatgaagc ctgggacggc ccgctctcct taaacggtaa
     4981 acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga aaggcatctc
     5041 ttttaaatcc tgcttactga acggttctgt tgaagtcgcc ccgcgtgccg gactggcgga
     5101 tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gcaaacggcc tgcgcgaagt
     5161 cgaagttatc taccgctcga aagcctgcct tatccagcgc gatggtgaaa tggaagaatc
     5221 caaacagcag ctgatcgaca aactgctgac ccgtattcag ggtgtgatcc aggcgcgcga
     5281 atcaaaatac atcatgatgc acgcaccgac cgaacgtctg gatgaagtca tcgccctgct
     5341 gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gaccaacaac gcgtagcgat
     5401 gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga aagcgctggg
     5461 tgccagttca attctggtcc tgccgattga gaagatgatg gagtgatcgc catgagcttt
     5521 aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct gttaatgcgc
     5581 ccggcgatct ccgcttctga aagcattacc cgcactgtta acgatattct cgataacgtg
     5641 aaagcacgcg gtgatgacgc cctgcgggaa tacagcgcga agtttgataa aaccacggtt
     5701 aacgcgctga aggtgtctgc tgaggagatt gccgccgcta gcgaacgcct gagcgacgag
     5761 ctaaaacagg cgatggcggt ggcagtaaaa aatattgaaa ccttccacac tgcgcaaaaa
     5821 ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt gccagcaggt cacgcgcccg
     5881 gtcgcttcag ttgggttgta tattcctggc ggctccgccc cgctcttctc aacggtatta
     5941 atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg ctcaccgccg
     6001 ccgattgccg atgagatcct ttatgcggcg cagctgtgcg gtgtgaagga cgtgtttaac
     6061 gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt gccgaaagtg
     6121 gacaaaatct tcgggccggg taacgccttt gtcaccgaag cgaaacgtca ggtgagccag
     6181 cgtctggacg gtgcggctat cgatatgccc gcaggcccgt cggaagtgtt ggtcattgcc
     6241 gacagcggcg caacgccgga tttcgtggct tctgatttgc tttctcaggc tgaacacggc
     6301 ccggactcac aggtgatttt actgacgcct gacgctgata tggcgcatca agttgccgaa
     6361 gccgtcgaac gccagttagc agaactgccg cgtgccgaaa ccgcccgcca ggcactgaac
     6421 gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tagagatctc caaccagtac
     6481 ggcccggagc acctgatcat ccagacccgc aacgcccgcg atctggtcga tggcatcacc
     6541 agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggtga ttacgcctcc
     6601 ggcaccaacc acgttctgcc gacttacggt tacaccgcca cctgttccag cctcgggctg
     6661 gcagatttcc agaagcgtat gaccgtgcag gaactgtcga aagaaggttt ctccgcgctg
     6721 gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga ccgcccataa aaatgccgtt
     6781 actttgcgtg ttaacgccct taaggagcaa gcatgagcac cgtgactatt accgatttag
     6841 cgcgtgaaaa cgtccgcaac ctgacgccgt atcagtcggc gcgtcgtctg ggcggtaatg
     6901 gcgacgtctg gctgaacgcc aacgaatacc ccactgccgt ggagtttcag cttactcagc
     6961 aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt gattgaaaat tacgcgcaat
     7021 atgcaggcgt aaaaccggaa caggtgctgg tcagtcgtgg cgcggacgaa ggtattgaac
     7081 tgctgattcg cgctttttgc gaaccgggta aagacgccat cctctactgc ccgccaacgt
     7141 acggcatgta cagcgtcagc gccgaaacga ttggcgtcga gtgccgcaca gtgccgacgc
     7201 aggacaactg gcaactggac ttacagggca tttccgacaa gctggacggc gtaaaagtgg
     7261 tctatgtttg cagccccaac aacccgacag ggcaactgat caatccgcag gattttcgca
     7321 ctctgctgga gctaacccgc ggaaaagcca ttgtggttgc cgatgaagcc tatatcgagt
     7381 tttgcccgca ggcatcgctg gctggctggc tgacggaata tccgcacctg gctattttgc
     7441 gcacactgtc gaaagctttt gctctggcgg gccttcgttg cggatttacg ctggcaaacg
     7501 aagaagtcat caacctgctg atgaaagtga tcgcccccta cccgctctcg acgccggttg
     7561 ccgacattgc ggcccaggcg ttaagcccgc agggaatcgt cgctatgcgc gaacgagtga
     7621 cgcaaattat tgcagaacgc gaatacctga ttgccgcact gaaagagatc ccctgcgtag
     7681 agcaggtttt cgactccgca accaactaca ttctggcgcg ctttaaagcc tccagcgcag
     7741 tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa caaccctctt
     7801 taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc gtcattgacg
     7861 ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc gcgatggaac
     7921 cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac tcgcctttga
     7981 accaggcgtg atcccggaac tgctgaagct gcaaaaagcg ggctacaaac tggtaatgat
     8041 caccaatcag gatggtctgg gaacacaaag tttcccgcag gcggatttcg atggcccgca
     8101 caacctgatg atgcagatct tcacctcgca aggcgtacag tttgatgaag tgctgatttg
     8161 tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac tggtggaacg
     8221 ttatctggct gagcaagcga tggatcgcgc taacagttat gtgattggcg atcgcgcgac
     8281 cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc gcgaaatcct
     8341 gaactggcca atgattggcg agcagctcac taaacgagac cgttacgctc acgtagtgcg
     8401 caacaccaaa gagacacaga ttgacgttca ggtgtggctg gatcgcgaag gtggcagcaa
     8461 gattaacacc ggtgttggct tctttgatca catgctggat cagatcgcca cccacggtgg
     8521 tttccgtatg gaaatcaacg tcaaaggcga cctctatatc gacgatcacc acaccgtcga
     8581 agataccggc ctggcgctgg gcgaagcgtt aaaaatggct cttggcgaca aacgcggtat
     8641 ttgccgcttt ggttttgtgc taccgatgga cgaatgcctt gcccgctgcg cgctggatat
     8701 ctctggtcgc ccgcacctgg aatacaaagc cgagtttacc taccagcgcg tgggcgatct
     8761 cagcaccgag atgatcgagc acttcttccg ttcgctctca tacaccatgg gcgtgacgct
     8821 acacctgaaa accaaaggta aaaacgatca ccaccgtgta gagagcctgt tcaaagcctt
     8881 tggtcgcacc ctgcgccaag ccatccgcgt ggaaggcgac accctgccct cgtcgaaagg
     8941 agtgctgtaa tgaacgtggt gatccttgat accggctgcg ccaatctgaa ctcggtgaag
     9001 tcagccattg cgcgccacgg ttatgaacca aaagtcagcc gtgacccgga cgtggtgttg
     9061 ctggccgata aactattttt acccggcgtt ggcactgcgc aagcagcgat ggatcaggtg
     9121 cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtact gggcatctgc
     9181 ttagggatgc aactgctggg tcggcgcagc gaagagagca acggcgtcga cttgctgggc
     9241 atcattgacg aagacgtgcc gaaaatgacc gactttggtc tgccgctgcc gcatatgggc
     9301 tggaatcgcg tttacccgca ggcaggcaac cgcctgtttc aggggattga agacggcgcg
     9361 tacttttact ttgttcacag ttacgcaatg ccggtcaatc cgtggaccat tgcccaatgt
     9421 aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg cgtgcagttc
     9481 cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct ggagatgtga
     9541 tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat cagggcgatt
     9601 acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat tacgccgcgc
     9661 agggtgccga agtgctgcat ctggtggatc tgaccggggc aaaagatccg gcaaaacgtc
     9721 aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt tccggtgcag gttggcggcg
     9781 gtgtgcgtag cgaagaagac gtggcggcgt tactggaagc gggcgttgcg cgcgtggtgg
     9841 ttggctccac cgcggtgaaa tcaccagaaa gggtgaaagg ctggtttgaa cgtttcggtg
     9901 ccgatgcctt agtgctggcg ctggatgtcc gtattgacga gcaaggcaac aagcaggtgg
     9961 cggtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg gaaacctatc
    10021 tgcctgtcgg cctgaaacat gtgctgtgta ccgatatctc gcgcgacggc acgctggcag
    10081 gctctaacgt ctctttatat gaagaagtgt gcgccagata cccgcaggtg gcatttcagt
    10141 cctccggcgg tattggcgac attaacgatg ttgctgccct gcgaggcact ggcgtgcgcg
    10201 gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag gccatcgcat
    10261 gctggcaaaa cgcataatcc catgtctcga cgttcgtgat ggtcaggtgg tgaaaggcgt
    10321 acagtttcgc aaccatgaaa ttattggcga tattgtaccg ctggcaaaac gctacgccga
    10381 agaaggtgca gacgaactgg tgttctacga tatcaccgct tccagcgatg gccgtgtggt
    10441 agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgttct gcgtggcggg
    10501 tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg ataaaatttc
    10561 catcaactcc cctgcgctgg cagacccaac cttaattact cgcctggcgg atcgctttgg
    10621 cgtgcaatgc attgtggtcg gcattgatac ctggtacgac ggcgaaaccg gtaaatatca
    10681 tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc actcagtggg aaacactcga
    10741 ctgggtacag gaagtgcaaa aacgtggagc cggagaaatc gtcctcaaca tgatgaatca
    10801 ggacggcgtg cgtaacggtt acgatctcga acaactgaaa aaagtgcgtg aagtttgcca
    10861 cgtcccgcta attgcctccg gtggcgcggg caccatggaa cacttcctcg aagccttccg
    10921 cgatgccgac gttgacggcg cgctggctgc ttccgtattc cacaaacaaa taatcaatat
    10981 tggtgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat gttaacagaa
    11041 caacaacgcc gcgaactgga ctgggaaaaa accgacggac taatgccggt aattgtgcaa
    11101 cacgcggtat ccggcgaagt gttaatgctg ggctatatga acccggaagc cttagacaaa
    11161 accatcgaaa gcggcaaagt caccttcttc tcgcgcacta aacagcgact gtggaccaaa
    11221 ggcgaaacgt cgggcaattt cctcaacgta gtaaatattg cgccggactg cgacaacgac
    11281 acgctactgg tactggcgaa tcctatcgga ccaacctgcc acaaaggcac cagcagctgc
    11341 ttcggcgaca ccgctcacca atggctgttc ctgtatcaac tggaacaact gctcgccgaa
    11401 cgcaaatctg ccgacccgga aacctcctat accgccaaac tgtatgccag cggcaccaaa
    11461 cgcattgcgc agaaagtggg cgaagaaggc gtagaaaccg cactggcagc gacggtacat
    11521 gaccgctttg agctgaccaa cgaggcatcg gacttgatgt atcacctgct ggtgctgttg
    11581 caggatcagg atctggattt aacgacggta attgagaacc tgcgtaaacg gcatcagtga
    11641 gttgcagggt aagcggatgc gatgttgttg tcgcatccgg caaaaaaaaa gggcaaggtg
    11701 tcaccaccct gcccgttttc tttaaagctg caaagattac ttcgcgttat aattacgcag
    11761 agcgttacgc cccagcacaa tccccgcgcc aaccatgccg cccagcagca ccgccagaat
    11821 caaggtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt tcatcacata
    11881 acggtaagca tgaatatcaa gatcatcaac ctttaagttt tcaatatcca acaggttttg
    11941 acgtgtctga tagtagtttg gtgagaacac caacggacgg gtcgcctcat gcttaatcat
    12001 cgactccagc gcttcgctcc ccaaaaggaa caacgtatct tgtgtcacat cttcagtctg
    12061 ctgaacctgt ggctttgcca cctgcgcctg attcgcatac tgcaacgctt cctgaatctg
    12121 acggatacgc agatctttct gctcctgcgc gactacttcc tgcgttctta aagagtcctg
    12181 caagtttttc cgtcccagag caatgttgtc tttgagatcc ttttctagct cttgattcac
    12241 tttatcatca acttgctgaa tgtattgggc caacttcatt tgtgctccct ctgcagtttg
    12301 cccaacataa gaaacagtca atggtaattg ctggttctta acagaaggtt cgatggtaag
    12361 tttttctggt tcttcctgat tatccagcgt ttctgctaat gcagagaagg cagaactgaa
    12421 gcgaccaatt aacgtctcct gtaaatccga tactttcggt gcagcctgac catagataac
    12481 attcatggca ttgttatagc cagcaatttg ccccacgtcg ggctgagtga taattgctgt
    12541 tgacgtccat ttctccttcg ctactgccaa atatccaata gccagggcaa tagccacaat
    12601 gacggaaatg atgattgtca tcttgccacg ccacaactgc actagtaaat caatcaaatc
    12661 aatctgttcc gggtcatggt tttgcccaga aacattatta ttttctactc tcatcattac
    12721 cctaactgac ggaaatttcc aagtccgcat agtttagcga taatcctagt gaatgctata
    12781 ggataagtga taaaaataat gaattattgg ggaggtaaca aaagacaatc agggcgtaaa
    12841 tagccctgat aacaagatgt tagtcgctgc caaagagatc gcgggtgtat accttatctg
    12901 ccacatcttt aagctcttct gccattcggt tagagataat gacgtcggct tgttgtttga
    12961 agatggcgag atcacgttcc agccgagagt tgaagaatga gtcttctttc atcactggct
    13021 cgtagatgat cacttcaacg cctttcgcct tgatacgctt cataatcccc tgaatagaag
    13081 acgcacggaa gttatctgaa ccgctcttca taatcagacg ataaataccc accacttgcg
    13141 gtttgcgtga caaaatggca tcggcaataa aatccttacg cgtgcggttt gcatcgacaa
    13201 ttgccgagat caggttattc ggcacagact gatagttcgc cagtaactgc ttggtatctt
    13261 tcggcaagca ataaccacca taaccaaacg acgggttgtt gtaatggttg ccaatacgcg
    13321 gatcgagaca aacgccttcg ataatctggc gagtattcag acctaaactt tctgcgtagc
    13381 tatccagttc gttaaagtat gccacgcgca tcgctaggta ggtgtttgca aaaagtttaa
    13441 tcgcttctgc ttcggtcgaa tcggtgaaca gggtcgggat attttgctta atcgcgcctt
    13501 cctgtaacaa cgcagcaaaa cgttctgcgc gttctgaacg ctcaccgatg acaatacgtg
    13561 aaggatggag attatcgtaa agggctttac cttcacggag aaattccgga gagaaaataa
    13621 tattttcagt acgatatttc ttatgcatcg cttcggtaaa accaacggga accgttgatt
    13681 tgatgaccat aaccgcataa ggatttatct caactacgtc tttaattact gattctacac
    13741 tggatgtatt gaaataatta gttttaggat catagtcggt tggagtggcg atgatgacat
    13801 aatcagcatc ccggtaggct tcatttttat ctaatgtggc attaaagtgt attttatctg
    13861 attgcaaaaa ctgctgaatt tccttatcaa caataggaga tatccgatca ttcagcatag
    13921 caacgcgtga cggtaaaata tctaatgcca caacctcatg attttgtgcg attagaagcc
    13981 cgtttgacaa gcctacatag ccagtaccgg aaatggtgat tttcatttca tgctctcaga
    14041 attaacttaa ctgtgaatca tgatgttttt agcatcctga taagagctaa atgttttaac
    14101 gctacgttat tagctggtca acgctacgaa aaacaattca gattttcttt ataagaatag
    14161 ctgatattta ttaagttaat attaagcagt gaaatttagt ctataagata aatggcaaaa
    14221 aaaaagcccg gtgcaatata cgccgggcct caattttact gttggttaaa tcagattaat
    14281 ccagccattc agtatggaac acaccttctt tatcaatgcg cttataagta tgtgcaccga
    14341 aatagtcacg ctgtgcctgg atcaggttcg caggcagaac agcggcacgg tagctatcgt
    14401 aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtact gcataagcaa
    14461 cgacatcacg cagcgcctgc tggtagtcat cggcaatttg cttgaagtac ggagccagca
    14521 gcaggttagc gatctgcgga ttttcggcat atgcatcggt gattttctgc aggaactgcg
    14581 cacggatgat gcagccagca cggaaaatct tcgcgatttc gccgtagttc agatcccagt
    14641 tgtattcttc agacgcagca cgcagctgag agaagccctg agcgtaagaa acgattttgc
    14701 ccagatacag cgcacggcga actttttcga tgaactcagc cttgtcgcct gctgactgag
    14761 cttgcggacc agagagaact ttagatgcgg caacgcgctg atctttcaga gaagagatat
    14821 aacgtgcaaa cacagactcg gtaatcagcg acagcggttc tccgagatcc agcgcgctct
    14881 ggctggtcca tttaccggta cctttgttag ccgcttcatc caggatcaca tcaaccaggt
    14941 agttaccgtc ttcatctttt ttagtgaaga tatctttggt gatgtcgatc aggtagctgc
    15001 tcagttcacc gttattccac tcggtaaagg tctgtgccag ttcttcgtta gagagattca
    15061 ggccgccttt caggagagaa taggcttcag caatcagttg catatcaccg tattcaatac
    15121 cgttgtgaac catcttcaca taatggcctg cgccatcagc accgatatag gtaacgcacg
    15181 gttcgccatc ttcagcaacg gcggcgattt tggtcaggat cggcgcaacc agttcatagg
    15241 cttctttctg gccaccaggc atgatggacg gccctttcag cgccccctct tctccgccgg
    15301 aaacaccggt accgatgaag ttaaagcctt cggcagaaag ctcacggtta cgacgaatgg
    15361 tgtcctggaa gaaggtgtta ccaccatcga tgatgatgtc gcctttatcg agatatggtt
    15421 tgagcgaatc aatagcagca tccgtgcctg cacctgcttt caccattaac aggatgcgac
    15481 gaggcgtttc cagagattca acaaactctt tcaccgtata gtaaggaacc agtttcttgc
    15541 ctggattttc ggcaatcact tcttccgtct tttcacggga acggttgaaa atagagacgg
    15601 tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc catcactgcc ataccgacta
    15661 cgccgatctg ttgctttgac attgtttact cctgtcaggg ggtggtcacc gccgagtgtg
    15721 cggcttaaat gtggtataga tgttaactca ctcatagaga tggtgaatag cattaccttt
    15781 gctataccac actgaatatc tcaccttttc cgtcttttaa aacttattta tatgaaatta
    15841 ctgaattact tgttcagtaa ctcaagaata agttttgtct tttcttccat cagcggcaca
    15901 tcaccgcgag attccacatt caaccgcacc accggttcgg tgttggaggt gcgcaggtta
    15961 aagcgccagt cggcaaaggt catgctgatg ccatcggtgc gatccaccgc cagcgcctcg
    16021 cggctaaaat gctgttccac gcggttaatc gcctcaacgg gttgcgccag tttgctgttg
    16081 atctcaccgc ttgccggaaa cgctgccatc cggtcgcgca ccagttcgcc cagcgttttt
    16141 cctttcaggc acaccagttc ggcgaccagc agccacggga tcatgccgct gtcgcagtaa
    16201 gcgaaatcac ggaagtaatg gtgggcgctc atttcaccac cgtagatggc gtcttccttg
    16261 cgcatccgtt ctttaataaa ggcgtgtccg gttttcgaca tcaccggcgt gccgcctgcg
    16321 gcagtcacca catcaacggt gttccaggag agacgcggat cgtggatgat cttcgcgccg
    16381 ggattttttt cgaggaatgc ttctgccagc aggccgacaa tgtagtagcc ctcaataaac
    16441 tgcccttttt cgtcaaacag gaagcagcgg tcaaaatcgc catcaaaagc aatgcccata
    16501 tccgcaccgt gtttgatgac cgcattgcgg gtgtcgtcgc ggcattccgg cagtagcggg
    16561 ttaggaatac cgttggggaa attgccgtcc ggcgtgttgt gcactttgat taattccacg
    16621 ggtgcgccaa gggctttaaa gcgggcttcg atggcgtcca ccaccggacc cgctgcgcca
    16681 ttcccggagt taatcaccag cttgagcggc gtgaggtttt tgacattgat ataaccgaac
    16741 aggtgatcaa cgtaagcgtc acgcagattg atttgctgat agcgaccgcg tttggtttca
    16801 tcaacgggag gaaagtcgtt ggcttctgcc agacgctgga catcgcgcag tccggtatca
    16861 ccgctgatcg ggcgagcccc ttcgcgcacc agtttcatgc cgttgtaatc catcgggtta
    16921 tggctggcgg taacttcaat gccgccatcc acgccgagat ggaacgtggc gaaatagatc
    16981 tcttcggtgc cggacatgcc aatatccagc acatcgacgc ccgcatcctg taaacctctc
    17041 gccagcgcca gttttaaggt ttcgctggtg aggcggacat caccgcctaa cacaatggtt
    17101 ttcggtttga gaaattcgcc ataggcgcga ccaatgcgcc aggcgatatc ttcattcagt
    17161 tcttcgccta atttcccgcg aatatcatag gctttaaagc aggttaattt tttcatgtcg
    17221 ttaccctttt tcaggcaata gttggccctg accgaaacgg ccaattttgt ttttgtcgtt
    17281 atttacatct cggtttcgca ttcattgcct gatgcgacgt ttacacccgt ccgtagcgat
    17341 ccgcgaaacg caccacatca tcctcttcga gataagagcc ggagcgcact tcaattaaat
    17401 cgagcggaat tttccccggg ttttccaggc aatgcatcgc ccccagcgga atataaatgg
    17461 actcgttttc accaagcagt ttgatatcac catcaatggt gacttttgcc gttcccgcga
    17521 caaccaccca gtgttccgcg cggtgatggt gcatctgtac cgacaagccc tcgcccggtt
    17581 tcacggtgat gcgtttcacc tggtagcggt cgcccgcgtc gatagagtca tatttgcccc
    17641 acgggcgata gacttcgcga tgcacccgat gctcatggcg accatcggct ttgatctgct
    17701 cgaccacttt tttcacatcc tgcaccgcgt tacggtcggc aatcagcacc gcatctttgg
    17761 tctgcaccac taccagatct ttcaccccga cggtggttac gaggccagat tcggcgtaca
    17821 catagctgtt ttcagttttg tgattaatca catcgccgtg gcaaacgttg ccctcggcgg
    17881 tgtgggcgct aatttcccat aatgaggacc aggagccgac atcgctccag cccgcatcca
    17941 tcggcaccac aacggcatct gccgtacgtt ccatgaccgc gtaatccacc gactcttccg
    18001 ggcaggcgag aaacgcttct tcatccacac gaataaaatc gagatccgga tcgacggcgc
    18061 tcatcgcttt ttcacaggca tcgagaatat ccggacgata ttttttcagt tcttcgagat
    18121 agcgtccggc gcggaacagg aacatgccgc tgttccagta atattcgccg cttgccacat
    18181 aggcctgggc ggtttccaga ttcggttttt cgacaaactg cgccacttca aaggccaccg
    18241 tatcctgctc acccgcagac acttcaccgc gacgaatata gccataaccg gtttctggta
    18301 gatccggcac aatgccgaag gtcaccagct tgcccgcttc ggcatatggc atggcattac
    18361 gcacggcggc acggaacgcg tcttcatcgg caatcacatg atccgccgcc aataccagca
    18421 ttaacgggtc gctctccggg ctatgacgtt ttgccgccag cgccgccagc gcaatggcag
    18481 gtgccgtgtt tcgccctgcc ggttcgagaa taatgttctc ggtaagtttg ttcagttgac
    18541 gcagctgttc cgcgacaata aagcggtgct gctcattgca aatcaccacc gggctttcgc
    18601 actccacgcc gttcaggcgg cagatggtgg tttgcagcat ggtgagatcg cctttcaggc
    18661 ataaaaactg cttggggtaa agtacgcggg aaagcggcca taagcggcta ccggagccac
    18721 ctgccatcac aactggatag agtttcgact gcgccataat tatccccgaa tatcatttat
    18781 aaattgacgt aacacgttct ctttatcgag tgtgcgttcg gcatattcac gtgccaccgt
    18841 gttgtgtttg ggcagcagga gcgcctgacg gatccccgcc accagcgcct cgaccgattc
    18901 cggttctacg caaaccgcaa tgcccggaaa ggtttcgcaa agctgcccca gttctgtgtg
    18961 ggcctctgcg gtaatcaccg cgttaccgcc taccgccaga atattggtca gtttcgacgg
    19021 caatacggca tctgccgcgc cgcgtttttg caccaccaga tggcaatcgc ccatcttcag
    19081 cagtgcgggt aaagcgtcat acgattgcag cgggaaaaat tgcatgttgc gcagtccacg
    19141 ctgctgcgcc attttttcca gccgcgcttt gccgccgccc tgcccgacaa tggcaaaaat
    19201 cagcggttca tcgcgcagac gatcggcagc ttcaataacg ttttccagcc cctgcttttc
    19261 accaatattg ccggagtaaa gaatgatttt tttgttatcc ggcaggtcaa gctggttacg
    19321 aagggcgtca acatcggcat ctgcaacatg ctgaaaacgg gcaatttccg accagttggg
    19381 gaagaagatg acgttttccg ccgccacgcc tttttcgatg gctttattca tcatcgaacg
    19441 cgaaatcgtg gagacgttat cgacgttatg cagtccgcta cgttcgaacg ccgttgccag
    19501 ctgtgccact ttgctgcctt tgccttttcc ggcaaggccc agccccagca tggcgtccac
    19561 ttcgtaatcc tgaatatgca gcacggtacg cgcaccagaa agtttcgcca gcaggcgcat
    19621 tcccggcgtg caaaacagcg ttggcaccac gccgataatg cgatccggct tccagcgacg
    19681 ttgcgccatc agaggaaaga aactgctgac ggcaaaactg ccgagatgta acaggcgttt
    19741 cagggtgctc ggctgttttg gcacgtacag cgggcagcgc cacaccgtgg cggccccctc
    19801 ttctcgtttg taccgccagg cggaatagtt ctcgcccacc tgccactgcg ggtagtaagg
    19861 cggtgcggta ataacccgca cctcatgacc ttgtgccgcc agccattcca ccatctcgcc
    19921 ggtgtatttg ccgataccgg ttaactccgg cgagtagtta atgccgtaga ccagtatttt
    19981 cataatccgg gtactccggc acgcttctca gcgagaaaat aggcgcggct gttagcgtga
    20041 acatcgttac tggcgagcag cgcgtccggc gtcagccagc ggtaatcgtc atgctgctca
    20101 tccggcagta tcagctcttc ttcagcgact ctgaagcgaa aaccgagcac cacatagtga
    20161 gtggtgaaat ccgtgccgga gaagttatcg tcataaaagt gctgccagac accgtaaaac
    20221 tggcctgctg ttatcggcag acgcagcccc agttccgcca tcgtcagccg ctcgaatgcg
    20281 gcttccagcg tttcgtcttt ctgcacgcgc cctcccggca caaaccagta accctgcgcc
    20341 gggcggttgg ttcttttgcc aagcagaaac tcaccgcgac tgttctcgac aataaagtcg
    20401 agagagacaa gcggggtgga gcgcactacc gtggcaaagt cttcctgacg taaaaacatc
    20461 attacccccg aaagcggtct tgattctcaa ggaaccactg gtaagtgctg gcaagccccg
    20521 cttccagtga gatttcgtga taccagccaa gctgatgcag gcgcgtcaca tccagcagtt
    20581 tgcgcggcgt accatccggt ttgctggcat caaaaaccac ccgacctttg taacccacca
    20641 ctttggcgat ggtttgcgcc agctcgcgga tagtgcagtc aacgcccgtg ccgacgttaa
    20701 tgtgcgacag catcggctgg gtgttctcca gccagacttc gtgcgccagc tccatgacat
    20761 gaatgctcgc cgccgccata tcatcgacgt gcaggaattc acgcatcggt gtaccgctgc
    20821 cccataccac cacgtccggt gcattctgtg ccgtcgcctc gtggaagcga cgcagcaatg
    20881 cagggatcac atgcgaatta ctcgggtgga agttgtcatg cgggccatac aggttggtcg
    20941 gcatgactga gcggtaatcg cgtccgtact ggcggttgta tgattcgcac agtttgatcc
    21001 cggcgatttt ggcaatggcg taaggctcgt tggtcggctc cagcgtgccc tgcaataact
    21061 cgctttctgc catcggctgt tttgccagtt tcgggtagat acaggacgac ccaagaaaca
    21121 gcagtttgtt cacgtcgttc tgatgcgcgg cgtgaatgat gttgctctca atcatcatgt
    21181 tctggtagat gaaatccgcc ggataggtgt tgttggcaac aatgccgccc actttcgccg
    21241 ccgccagata gacctggtca atgcgttcgc tggcaaagaa atcatgcacc gcgcggctgt
    21301 ccagcaggtt cagctcgtcg cgggtgcgta ataccagttc cacatcaccg cgctgttcga
    21361 gctgccgcct gatggcagaa ccgaccatcc cacgatggcc agcgataaaa atgcgttgtt
    21421 tactcatgct tatgactcca gcgcgatcgc cacgtcgtag ccgtgagatt tcagcagaga
    21481 gtgttttttc gccgcttcga ggtcattagc caccatttca gacaccatct ctctgagggt
    21541 gatttccggt ttccagccca gtttttcgtg cgctttggtc gggtcgccga gcagcgtttc
    21601 aacttcagcc ggacggaagt aacgcggatc aacggcgata atcacatcac ccggtttaac
    21661 gcccggcgcg tcatgcccgg tgacggaaac cacaatgccc ttctcttcaa cacccgtgcc
    21721 ttcaaagcgc agtttgatgc ccagctgtgc cgctgccatt tccacgaact gacgcacgga
    21781 gtactgaacg ccggtcgcga taacgaaatc ttccggctgt tcctgttgca gcatcatcca
    21841 ctgcattttt acgtagtctt tggcgtggcc ccagtcacgc agggaatcca tattgccgag
    21901 gtacaggcac gactccagcc cctgggcgat gttggcgatt gcgcgggtga ttttgcgggt
    21961 aacgaaggtt tcgccgcggc gcggggattc atggttgaag agaattccgt tacaagcgta
    22021 catgccgtag gattcgcggt agttaacggt gatccagtag gcgtacagtt tggcgaccgc
    22081 atacggagat cgcgggtaga acggcgtggt ttctttctgc ggaatttcct gcaccagacc
    22141 atacagttca gaggtggaag cctgatagaa acgagttttc ttttccagac cgaggaagcg
    22201 gatcgcctcc agcaggcgca gcgtacccat cgcgtcgacg tcagcggtat attctggtga
    22261 ctcaaaagag accgcaacgt ggctcattgc gcccaggttg tacacttcat ccggctgtac
    22321 ttcacgcaaa atgcgcgtca ggttagaggt atcactcagg tcgccataat gcagatggaa
    22381 tttcgggttg caggtgtgcg gatcctgata aatgtgatcc acgcgctcgg tgttgaatga
    22441 cgatgcgcga cgcttaatac catgcacctc gtaacctttt tccagcagaa actctgccag
    22501 gtaagaaccg tcttgtccgg ttacaccggt gatgagagcg acttttgaca tgtcttattc
    22561 ctctgtattt ttgaatttat tcagtttcaa cgcgttcgcg tatcaccact gcgggattcc
    22621 cccggcaaac cacatttgcc ggaagcgatt taaaaacact gcttcgtgca cccacgacgg
    22681 tgccgtcgcc gattgtgacg cctggggcaa caaagacatc ggttgccagc cagcatttct
    22741 cgccaatcac aataggcgtg gcgttaatgg tgaaatgttg acttgcatgg tcgtggctac
    22801 cggtgcataa ataacttttt tgcgatatca ccgaatgtgc gccaatggtt atttcaccga
    22861 gggtatataa attgacgtca tcgccgaccc acgcgtaatc acctaaggtt aatttccacg
    22921 gataggtaat ttttactgac ggacgaataa ctacgttttt tcctattttt gctccgaata
    22981 aacgtaataa aaaagcccgc cagcgataca atacttgtgg cgaccaggca aatattgttg
    23041 cctgtactgc ccaccataat tgcactttaa tagcgttgcc gccccggaac cctttcggca
    23101 ccgagaaacc gcttaaatct tgcattggtt ttccttatat tcagactttg ttatataagg
    23161 ctttcgtctt tgaggtagta cgttggcgta aatgccagga taattcagcc caaaagccag
    23221 gcacatgtaa tatttgtcgt tggacttttt tcgcgtcagc acacaattcc atattattgg
    23281 tggtagatac cccacccatg gaaaattcag acaccaggcc attgagtttt ttaaatgcat
    23341 aaccggcttt atacattttg gctgccagcg cgtagtcgga agaaacttta tattccaggt
    23401 cataacgcca ttttttcaag ccggatacag ggaaaaatat cgcctgatga ctggcgggca
    23461 ggctgtgata aatataccag cccggtttgg cgctacgttt aattttatgc ccgtcgccaa
    23521 aatccagcag cgcatcgccg gtgatcatca tgttatcttt ttgcattttt aacttacgga
    23581 caaaatttgc ggcatcctga tgaaaaatat cgcccgaatt gagaaacaac gcgaacttgc
    23641 cttgtgccat cgtaatgcct ttgttcatgg cgtcgtagat accgttatct ggctcgctga
    23701 caaagcgtag gttatagata ccattgagat tttccagata ctcacgggtg ccgtcattgg
    23761 aaccgccatc gacaacaatc cattcgaagc tgatatcttc cgcctgcgcc agatgcgcca
    23821 gcgaggcatg tgttttgact atcccttcga ggttacgaaa cgcgacagtg attatgctaa
    23881 gcaacatgtt cttattctta cctcgtaata tttaacgctt ttcgcaaaat aaatggacag
    23941 acgattaaaa atgcatattc cgggctaaat atcgaaccgg taaaaaacag cgataccgga
    24001 gtaaaaagat ataattgcac gcgaaaattg cggttatcac caaaagcgtt gatcatcatt
    24061 tttatcactt tccccatgta ccacagcgat aaaaacactg cgaaccagga aaaataaata
    24121 atcagcagat acaaaccatt gtctatggtt tttccgacat ccgcaccgtt aaatattccg
    24181 aatgatgcga cgtattcata aagtgagcca aatctgacta caccgtcaat atgggtcaaa
    24241 gaataaccga ccatcaccag cggaccgaca atacgataat acgatgacga tccttccgta
    24301 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aaccaggaat
    24361 acagccagag aaatcaatgc caacggtaac tttttcttaa tcgcctcttt attcagatat
    24421 tgaaatgccc actccagcaa ataaaacagg ataaaagtca taacccctga aaacgatccg
    24481 gataatatta tccctgcgag aatcatagca tcggttttag gcgttttgat accaaactgt
    24541 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
    24601 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagta catagctgtt gctgtaaatc
    24661 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
    24721 tccaccgccg ccagcgccac caccagcccg atcaccagat aaaagaaacg taatatcttg
    24781 cgatggttgt gtggcgaaat cgttttaaag cgaatactcc acaccatgcc aataatgatc
    24841 acaatataga caaacagcat ggttgaagtg acgtatttac tggcatccag cgactgacca
    24901 aacagatagt taaacgccgt gagtcccgca ccaatcccta aggcaatcat caatttttta
    24961 acgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
    25021 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagcggcagc
    25081 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
    25141 gtaggtggcg cggctgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
    25201 gatttccggt ttgcttaact gcaccagttg cagcacgtct tcttcgtcga cggttttacc
    25261 gccggatttt tgcaacactt cccgtgccgc gtcgctatgg gtggcaatca ccggcacgcc
    25321 aattgataac gcctcacaca aaatcagcgg gtagttatcg acgcgagaac tgaataccag
    25381 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
    25441 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
    25501 gcccagcgcc atcatctcac gcaccagttg ctggttagtc ttgccgtcgt aacgcaagtc
    25561 atgcgccacc accgcgattt tcggcttgcc ctgggtttcg cgcaccggag gcaagtccgc
    25621 cagaatcgct tcggttgcca tatcaatacc attattgata atccggcaac gccctggacc
    25681 gtacaggcta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
    25741 cgccagcatc tcacggaata actggcgttt gcccgccacc agctggtgtg cgcgatcaat
    25801 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
    25861 gcaaccgtcg gtaaaggcgc agcgtccggt aacactccag tggtcgtgca gcgtccagac
    25921 cagagtgacg tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctcttaag
    25981 atttagccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
    26041 aatggtgcga tataactcat tgaaattgcc aaacagatcg cgattaaaca gacgaaacag
    26101 ggcaatattc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
    26161 atggctgacg ctctctttgc cgcctttgcc gtaaccgtag acaaaatgtg acgccagccc
    26221 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
    26281 tcgcacatta aattgcagaa tattcattta attaccttca ctcgcgcttt ttctcccacc
    26341 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
    26401 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
    26461 atgtgtggac atgccatgtt atcggcacca cgattgccga tagtgacgcc gtggcgaatg
    26521 gtgaaatcat cccccgctac cacgttttta ttgatcacta cggcgtaacc gtgatggata
    26581 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
    26641 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
    26701 cgccacaccg aacaaaaatg agcaacacga taggcaagaa ccatgcagca cgggcgtaaa
    26761 ctccagctgt tggcacgcag atcttccagc atctttagcg cccccgaata ccatcagcca
    26821 gacgtttgcc gttacgcacc gacagcagcg tcagcaaagt gcgccaggtc atacgtttat
    26881 tgcgaatctg gtagagggta aacagctgat attttttgct tgcacggtca aatttatctt
    26941 tgtgctttcg gtaaaagtgg aagtacccgg agaatttttt cggtgacgag gtgatctgca
    27001 tttcaccgtg attgatatgc aggatctgcg tcgcttcttc cactttccac ggttcgccgt
    27061 actccaccac catccgcagg aagatgtcgt aatcctgtgc tgctttcagt tcggtatcga
    27121 acaaacactc tttgaaacgc catgcccaag taaagacctg attgccaatg atattgcgtt
    27181 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctt gccggttggg
    27241 aataaacttc tccctggcat acgtagtcgt tagcgtacaa aaaggcgtga gtgaccagtt
    27301 gctgtttatg ggcgaggaag acgctcagac ggttgggtgt ccattcgtca tcgtcatcga
    27361 tcccggtaat gtattcccct tgcgccagca taatcgcctg gttacgtacc gcgcacgccc
    27421 cgctgttaat gtcgttgtga atgtaggtga tacgcggatc gttgagggca gtgacgtact
    27481 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
    27541 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggtcg
    27601 gcatatagat tgaaatcagc ggattctctt tcataggttg ttctcccggc ttatgagctg
    27661 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
    27721 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
    27781 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
    27841 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcatagc gcgccaccat
    27901 taacgtggtt ccagcgtgac gcccgacaat tgctgcatcg gtcactgcca gaatcggcgg
    27961 cgtatcaatc aacaccaggt cgtagttttt actcgcccag ctcaccaatt cggcaaagcg
    28021 ttcgctcatc aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
    28081 tttggcaata gaggtcggtt tagctgcggt agtaatatcg ccctggccaa tcagaatttc
    28141 cgacaggcca ttgacgttat tggtacccaa cagctcgtgg gtgtagcctt tgcgcatatc
    28201 gcagtcgatc aacaacaccc gtttattggt ctgactgatc accgccgcca ggttggcgca
    28261 gacaaaggtt ttaccgattg acgggctcac ccctgtcatc atcaacacat tgttctgcgc
    28321 ctgcatcatc gcgaagtgca gactggtacg cagactgcgg atggcttcaa tcgccagatc
    28381 ggttggattc cccaccgcca gtagctggct ctgtttatag cgtttaatcc ctttgatggt
    28441 tttgacgcta tcgcgcgctt tctgccattc cgacagcggg atgctggcat agacgctgat
    28501 accatgttct tccagcacct gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
    28561 cacacccacg atagagagca tcaggccgag gataattgcg ccgaggataa tcagtccttt
    28621 cttcggtttc agcacgccag gctgggtgat tgccgggtca acaatgcgca catcgccgac
    28681 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
    28741 ctgaccagac tcgacatcgc gggtcaggcg cacaatctcc tgctgggttt tcggcatcgc
    28801 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
    28861 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
    28921 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttccag
    28981 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
    29041 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
    29101 cgccgatttg cgctcaatat tttgttcctg atagttacgg gcgatgctgt taagaatgtc
    29161 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
    29221 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
    29281 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
    29341 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
    29401 ggtgtagttt ttattgtcca gcacattaag cgtaaacacc tggcccgcca tctctttcgg
    29461 gcggttaaag gtagtcactt tcaccgtctc gttctggcgt cccatcagtc gctcccagcc
    29521 cgccccgaaa atcgggaacg tgtttttgct cactgcaata tcgaggtcga gatcgtccac
    29581 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
    29641 tttgttggct aatgccgaac caatgtcctg cactaacgaa ttgccgctgt tttgctcgat
    29701 ttgtaccagt gcgtcggcac tataaatcgg cgttgcgaag aaggtgtaaa ccacggcaca
    29761 gagggcaaat acagcggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
    29821 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
    29881 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
    29941 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgcgatacgg atcggggatt
    30001 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc aggtgccatc
    30061 tcgcataagc gttcgatatg gcgcttttcc atggtcaaaa tcaggtcata gttgcgacac
    30121 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
    30181 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
    30241 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccgtcgggga acggcaaata
    30301 ttgccgacac agacaactaa tatgttgtta aacatgacga ttaccagtta tgaatgtcgc
    30361 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg caacaactga ttgatcagac
    30421 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
    30481 attctgtcgc catcaccagc gacgtggcat cggacatatc cagctggtag atattggcaa
    30541 tcttgccatt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
    30601 tggaggtggt catgtcgata ccttcagcat tacccagggc ttcagtcagg gtcatgccgc
    30661 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
    30721 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
    30781 cgccgttttg catcagcgcc tgcaaagaaa tgcgttcttc acgaccattg tgtgttagca
    30841 ctacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
    30901 tagtcagtgg cacgttggtg atcgcctgtt gaccggattt attcacctga ccggagatat
    30961 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
    31021 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
    31081 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
    31141 tggagctgcg gtactgaccg gctggcgtgg tgagttccgg gtgatcccag acggtgacat
    31201 tcagaacgtc gcccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
    31261 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
    31321 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
    31381 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
    31441 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
    31501 agagtaatta tcctgtgcac tattaatagc aatatcgcca tgcacattta ccttgcagtt
    31561 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
    31621 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
    31681 tagccaccaa tacactgatt tagcttaatt tttcaaaccc tctctgcata caatcgttga
    31741 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
    31801 tattggctct ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
    31861 acaattcggc aattggctat ttttaagaat tatattaagt gtcattcaat atggttttta
    31921 ggagtttctt taggttgaca atatttaata ttgcgcctcc acatgcgata tttcttaaat
    31981 aatgttttat tattaccact tttaattcag ggataatgtg aggttattac cctcaaataa
    32041 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
    32101 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
    32161 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
    32221 tggtctttat tgccatcctc gccgaaaaac taccgccgag gcagcgcgac cgcgcacggg
    32281 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctggcgtca atctcctggc
    32341 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
    32401 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
    32461 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
    32521 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
    32581 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
    32641 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacg atcgttattc
    32701 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
    32761 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcgctca
    32821 atcagttggc tatctttaac cggcgacgtt ttctttccgc taaccagacg ctgcgccagc
    32881 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
    32941 ccgaaaccgc gtccatgctg gtggatcatg gtaaccagca gatctttaat ccgcaggaac
    33001 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtcagcagc attatgacgt
    33061 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
    33121 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
    33181 tcggtgttgt tcacgttatc gacctgctac aacagtctct gcgcggcgaa ccgctcaacc
    33241 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttacctgccc
    33301 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
    33361 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgtcggtaac ttaccgaacg
    33421 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
    33481 cgaatggtca tatgccgctg gaagatctgg tgcaatatgt gccgctgccg ctggatgaaa
    33541 aacgtgaata tcacaccatt gccgggctgt tgatggaata tttacagcgt attccaaaac
    33601 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
    33661 gcgtgcagaa ggtgcagatt attccgctgc ataaggatgg cgagttggag tacgaggtgt
    33721 gatggtatcg gtcattttac ccaatagccg aaaggaataa gatgagggaa ccgcgccagc
    33781 ggcgatttac cgccgggagc ccgggttgcc agggtggtgg cgattgagcc accctggcac
    33841 gttcaccggc ttgagcgttc catagtagcg aggaacatca agtgaacgga acagccttcg
    33901 atcttacata ttcagaatat aatgatgccg ccacgaacgc ctgatgcgac gctttacgcg
    33961 tcttatcagg cctacaggac tggtcacctg gtaacctaca tcttctccag caacttcttc
    34021 acatccttgc cattgcggga atctttattc cgctcggccc agtcattcag gcgacgtttc
    34081 gcttcgtcct gtagatgttt gcgcagtaac tgatccactt gcaggctgta attgagttgc
    34141 tgccagttgc cataaacccg cagcggtact ggcgtttctt tcaggaaatc aatcagttta
    34201 ctttccccgt tccagccacc cacgacccga atatcaaact gggtatcgca ggtttgatct
    34261 gccagattca acatgccttc ccctgtcagc gccagcacag gcgattgacc ttgcatgtcg
    34321 tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc aagccgcgtc
    34381 acgttatcaa agttttcagc ggccttcaca tcaccgccat tacgctctac cgcttgctga
    34441 atcatctgct ggaagttcat cccttccatg cgcgtgtcgg tcatttcgac atgtgcctgt
    34501 ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa atcaccagcc
    34561 agtgacattt ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat ggtaccaatc
    34621 tcaacgtttt ccagccgtgg ctggaagttt atccgcggat ttattgatgt cgcgtccagc
    34681 gtgcccggca gtgaaacctg tccaccgtta agtttgccct gcagttgagt aatttccagc
    34741 aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc gcgccagcgc
    34801 acgttactgg cctgcaacaa aatatcagcc gtaaagcctt gcagtccctg ataggccggt
    34861 tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg attggctctg cccctgctgc
    34921 gcggcaccgt tttcaccatt cgcggtttca ttaagcggga tgaggttgtc aagattcagt
    34981 tgcgggaatt gcagtctcag ctgccattcc ggtttctctg tcagcgtgac ctgtgcttgc
    35041 ccgctcagcg tactgtcatt ggcggtcaag ctgatttggt taaatgaaag gcgtttatgt
    35101 gactcctgcc actgggcctg gaaactcccc tgcccctgaa taccttgttt tggtaaatcg
    35161 gcaccctgca actgccagtt aatttgttca atagccgccg ttaaatcatg cggataatca
    35221 gaagcatcca ccgtaccgtt aagggatatt gtgagatcgc gctgatcgcg attaacccgc
    35281 ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag gcggatattg
    35341 cggattgtca cctgctcgtc atcttcatgc tggaacacca gcacgctgtc cgccacctta
    35401 agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa ggtattgtcg
    35461 cgcggtgcaa ccggagcgtc ttcactgcgc accgcttccg tctgcggcgt cagttggatc
    35521 actgcccctt ttagcatcac ctgcttaacg ctcagttgat gactcagtag tggtaaaagc
    35581 gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc ctgggcggtg
    35641 agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg cagtggcccg
    35701 tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata gtcgcggaaa
    35761 tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac gaccaggagt
    35821 atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa ttagtcttta
    35881 tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc acggcggttg
    35941 taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca gcgcaccaat taacatgccc
    36001 ggacgcagcg ccagcggcag cttaccggag ttgtagaact ccagcacaat gcaaccagac
    36061 cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg cgccaaagag
    36121 gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc cagcgtcacc
    36181 gccagcgcca gctctcctgg gtgaagatag aacgcctcgc cctcgtcgag aacgatctca
    36241 tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct cagatcgata
    36301 aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgtac atccaccgtc
    36361 gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc ttcatcaagc
    36421 caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcgc gcatcactcc
    36481 cttaacgcca attacgtcaa gggcatacca ggttatcact gaacggtaca caatttatca
    36541 agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga tgcggttttt
    36601 cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga attgcaggaa
    36661 catcgggcgc acggtttttt gatattgcgc catcacggaa tccattgaac gcccacgctc
    36721 gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat caacgaaaat
    36781 ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc aacaaaatgc cttcgagaat
    36841 gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt caacatagct
    36901 gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct caagcagcag
    36961 gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt ccatcgacag
    37021 atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt catcaccgac
    37081 ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg aagccgatgc
    37141 gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt tagcgacctg
    37201 attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt atagggattt
    37261 catccgtctg ataccagtcg aatagcgttg ccgcgcgctc agagttaatt gttgacaaag
    37321 aattcccggt ggcaaattac gttgatcagt tttatgtaag gtaaaaaatg ttatacgcag
    37381 ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa aatgtaaaac
    37441 ttttgtacta gcataaacac agaaacgaat actggcgacc tggtcttgcg gataaagcgg
    37501 taatgagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg cttcacctgg
    37561 tcagtttagg tttagtctcg tttatcttta cccttttctc gcttgagctt tcgcagtttg
    37621 gcacccaact cgccccactg tggttcccga cgtccatcat gatggtggcg ttttatcgcc
    37681 atgccgggcg catgtggccg ggaattgcgc tgagctgttc gctgggaaat atcgccgcat
    37741 ccatcctgct tttttccacc agctcgctga acatgacctg gacgaccatc aatattgttg
    37801 aagccgtggt cggggcagtg ctgctacgta aattgctgcc gtggtataac cccttgcaaa
    37861 atctggctga ctggctgcgt ctggcactcg gcagcgccat tgttccgcct ctgttggggg
    37921 gtgttctggt tgtcctgctg acgcccggag acgatcctct cagggcattt ttgatatggg
    37981 tactgtcaga atccatcggc gctctggcac tggtgccgct gggattgtta tttaaaccac
    38041 actatctgct gcgccatcgc aacccacggt tgctttttga gtcgctgctc acgttagcca
    38101 tcacactgac gttaagctgg ctttcgatgc tgtatctgcc gtggcctttt actttcatta
    38161 ttgtgctgtt gatgtggagc gccgtgcgcc tgccacgaat ggaagccttt ttgatcttcc
    38221 ttaccacggt gatgatggtg tcgctgatga tggccgcgga tccctccctg cttgctacgc
    38281 cgcgtacgta cctgatgagc catatgccgt ggctaccgtt tttgctgatc ctgctgcccg
    38341 ccaacatcat gaccatggtg atgtatgcct ttcgtgcgga acgcaaacac atttccgaaa
    38401 gcgaaacccg ctttcggaac gcgatggaat attccgctat cggtatggcg ttagtgggca
    38461 ccgagggaca atggctgcaa accaacaaag cgctctgcca gtttctcggg tacagtcagg
    38521 aagagctgcg cggactcacc tttcagcaac tgacctggcc ggaggatctc aataaagatc
    38581 tccaacaggt tgaaaagctg ataagcggtg aaataaacac ctattcaatg gaaaaacgct
    38641 actacaaccg caatggcgat gttgtctggg cgttgcttgc cgtctcactg gtgcgccaca
    38701 cggatggcac gccgctctat tttatcgctc agattgaaga cattaacgag ctaaaacgca
    38761 ccgaacaggt caatcagcaa ctgatggagc gcatcacgct ggctaacgaa gcgggcggga
    38821 ttggcatctg ggagtgggag ttgaagccga atgtttttag ctgggataag cggatgttcg
    38881 agctgtatga aattcctcca catatcaaac cgaactggca ggtgtggtac gagtgcgtgc
    38941 tgccggaaga tcgccagcat gccgaaaaag tgattcgtga ttcgttgcaa tcacgctcgc
    39001 cctttaaact ggaatttcgc attaccgtaa aagacggtat tcgccatatc cgcgccctcg
    39061 ccaaccgggt actgaataaa gaaggcgaag tcgaacgtct cctcggcatc aatatggata
    39121 tgaccgaagt gaaacagctt aacgaggcat tgtttcagga aaaagagcgc ctgcacatta
    39181 cgcttgattc catcggtgaa gccgtggtct gtattgatat ggcgatgaaa attaccttta
    39241 tgaatccagt ggcggagaag atgagcggct ggacgcagga agaagcgtta ggtgttccgc
    39301 tcctgacggt gttgcatatt acttttggcg acaacggacc attaatggag aacatttaca
    39361 gtgccgacac ctcacgttcc gcaatcgaac aagatgtggt gttgcactgt cggagcggcg
    39421 gcagttacga cgtgcattac agtattacgc cgttaagtac tctggacggc agcaatattg
    39481 gttcggttct ggtgattcag gacgtcaccg aatcacgcaa aatgctgcgc cagctgagct
    39541 acagcgcctc ccatgatgca ctgacgcatc tcgccaaccg cgccagtttt gagaaacaac
    39601 tgcgtatcct gctgcaaacg gtaaacagta cgcatcagcg acatgccctg gtgtttatcg
    39661 atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca tgcggcgggc gacgctttgc
    39721 tgcgcgaact ggcgtcgttg atgctgagta tgctgcgctc cagtgacgtg ctggcgcggc
    39781 tcggcggtga tgaatttggt ctgctgctgc cagactgcaa tgttgaaagc gctcgtttta
    39841 tcgctacacg tattatcagt gctgtgaatg actatcactt tatatgggaa ggccgggtac
    39901 atcgggtagg tgccagcgcc gggattacct tgatcgatga caacaatcat caggcggcag
    39961 aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa aaatggtgga cggggccggg
    40021 tgacggttta cgaaccacag caagctgccg cacatagcga acgggcggcg atgtcgcttg
    40081 atgaacagtg gcggatgatt aaagagaatc agttgatgat gatcgcccac ggtgtcgctt
    40141 cgccgcggat cccggaagcg cgtaatttgt ggctgatttc gcttaagctc tggagttgcg
    40201 aaggcgaaat tattgatgaa caaacatttc gtcgtagctt cagcgatccg gcacttagcc
    40261 atgctcttga ccgacgggta ttccacgaat ttttccagca ggccgccaaa gcggttgcca
    40321 gtaaaggcat aagcatcgcc ctcccccttt ccgttgccgg tttgagtagc gccacgctgg
    40381 tgaatgatct gcttgagcag ctggaaaata gccctctacc accacggtta ttacatctga
    40441 ttattccggc tgaagcgatt ttagatcacg cagaaagcgt gcaaaaactg cagctggcgg
    40501 gatgtcggat agtgctcagc caggtgggcc gcgatctgca aatcttcaac tcgctgaaag
    40561 cgaatatggc agattacctg ctacttgatg gtgagttatg cgccaacgtg cagggtaatt
    40621 tgatggatga gatgctgatt acgattattc aggggcacgc tcagcgactc gggatgaaaa
    40681 ccatcgccgg gccagtcgtt ttacccttag tgatggatac gctttctggc atcggcgtcg
    40741 atctgattta tggtgatgtg attgccgatg cccaaccgct ggatttgctg gtgaatagca
    40801 gttatttcgc gattaactga gagagcgttt gcccattgcc tgatgcgacg ctaacgcgtc
    40861 ttatcatgcc tacaaatcgc tcattcccca gaccggataa ggcgctcgca ccgcatccgg
    40921 cgaccaacgt catgcttcgt ctggttgcca gccttccgta taccagatat gcaacagcgc
    40981 ataagaacgc cagggcttcc agcgctcggc ataacggcgg atttgtgccg gtgtcattcc
    41041 cggaaatcgc cgtttaatca gataatcatc cggcagaaaa acatctttcg cctgccagcc
    41101 acgcaaagca aaataattcg ccgtccagcg cccgataccc ggaaaagttt gcagcgtttt
    41161 catcgcctgc tccacatcgc ccggaattgt cattggtaag ctgccctcca gcgccgcatt
    41221 tgccagatga atcagtgcct ctgcccgttt caacggcatg cctaacgctt ttaatgcctg
    41281 cgggtcggct gctgccagcc gctgaggcgt ggggaagcag atatactccg gaaaatcatc
    41341 cagccgttcg ccataaagct gtaccacttt ggcagtcaat tttgccgcca tcgccacgct
    41401 caccagttgg cctaaaatcg cccgcacacc ctgctcaaaa gcatcaatac agccgggtaa
    41461 acgcaatccg ggccgccccg cgcctaactt tcccaacgca ccgttaacaa tctgcgggtt
    41521 acattgcaga tcaagcaggc ggctcatttt cgccagacac tctgcggcaa caggttctaa
    41581 acctgcactt aaatttatgt gcagagtatg gcgggctata tccggaatag cagtcactac
    41641 gccgcgatat tcgcccaccg ccagactacg ggcataataa ttgtccgcga ccgtttccac
    41701 accgctcacc gcacgggcgg cgagaaatcc caacatccac gaccagtcat acggcggctg
    41761 ccagttcagg gtatacatcg catctccttg ttaatccgct ttcagcataa acgttattca
    41821 gacgttacgc tttgctttca tattccggtt gtcgcgacag caacatttcg ctaaagtcac
    41881 gccccttctt caccggcatg gggattaatt cgtgtttatt ggttttgatt acggtacagc
    41941 aaactgttca gtggcggtca tgcgtgacgg taaaccgcaa ttactgaaaa tggaaaacga
    42001 cagcacgctg ctgccttcaa tgctttgcgc gccaacgcgt gaatcggtaa gcgaatggct
    42061 gtaccgccat catgatgttc cggcagacga cgatgaaacg caggcgctgt tacgtcgggc
    42121 gattcgttat aaccgcgaag aagatatcga tgttacggcg aaaagcgtgc agttcggtct
    42181 ttcctcactg gcacagtaca ttgatgatcc agaagaagtg tggtttgtga aatcaccaaa
    42241 atcgttcctc ggtgccagcg gcttaaaacc gcagcaggta gcgctgtttg aggatctggt
    42301 ctgcgcaatg atgttgcaca ttcgccagca ggcgcaggcg cagctgccag aagcgattac
    42361 tcaggcggtg attggtcgcc cgatcaactt ccaggggctg ggcggcgatg aagcaaacgc
    42421 ccaggcgcaa gagattctgg aacgcgcggc gaagcgtgcc ggattcaggg acgtggtatt
    42481 ccagtacgag ccgatcgcgg ctgggctgga ttacgaagcc accttgcagg aagaaaaacg
    42541 ggtgctggtg gtggatatcg gcggtggtac gactgactgt tcattgctgc tgatggggcc
    42601 gcagtggcgt tcgcgtctcg atcgtgaagc cagtctgctg ggtcacagtg gttgtcgtat
    42661 tggcggtaac gatctggata tcgccctggc gtttaaaaac ctgatgccac tgctgggtat
    42721 gggtggcgaa accgaaaaag gcatcgccct gccgatcctg ccgtggtgga atgcggttgc
    42781 catcaacgac gtacctgcgc agagtgattt ctacagtagt gccaacggtc gtctgcttaa
    42841 cgatctggta cgcgatgccc gcgaaccgga aaaagtggcc ctgttacaga aagtctggcg
    42901 tcagcgttta agctatcgcc tggtgcgtag cgcagaagag tgcaaaattg ctctttcaag
    42961 cgtagcggaa acccgcgcct cactgccgtt tatcagcaat gaactggcta cgctgattag
    43021 ccagcgaggg ctggaaagcg ccctcagcca gccactggcg cggattctgg aacaggtgca
    43081 actggccctg gataacgccc aggaaaaacc ggacgttatc tacctgaccg gcggtagcgc
    43141 ccgttctccg cagattaaaa aagcgctggc agaacagttg ccgggcattc cgattgcagg
    43201 cggcgatgac tttggctccg tcaccgccgg gctggcacgc tgggcggaag tggtgtttcg
    43261 ttaattgaaa aatggctcag cagcggctta aatctgcctg cgcctgagcc agtttttctg
    43321 ctgcctgacg taacggttcc atcaacggca tggtccgtgc tggagcatga aggcggaatc
    43381 gctgcaactc tgtcgcgccg actgtcaacg ccgattccag ccgatgtcgt ttggcggtca
    43441 ttttcgccat taccgcttgt ctgtctgctg gcgttatcgc ttcattcggc ctgaacacaa
    43501 aacggcgttc acagttcgct ttccagtcga taaccgcctg ggtcagatgg tcgccaaacc
    43561 ctttcacttg cttaacgcca cgacgggtaa catctgcggc tgtttcaata ccaaaagacc
    43621 gtaacgccgc tttacgcgca gggccaacgc cgggaataga ggcaacatca ataaaaaatc
    43681 cctccagaaa cttctgcttc tgccgttccc ttgcagtgtc atgaagtgct gccagagcgc
    43741 gtttctcttc ttccggtaac ccgagaattt cgtccttcat tttttcgagc atcgtccgtt
    43801 tggcgataaa accttccagg ccgcctacct gctggatctg gcggactaaa tggtcatagt
    43861 ccattttcgc acgattaaat gcctcacggc gttgctgaac ttctgctttg tacgctttgc
    43921 ttgtcaaact gccaataatc cagatagccg ccagcacagg aaccaaaata atataacgcg
    43981 gttctgcctg aaggccgcaa agcagcagcg ataatgctga cagtgcgatc tcaagcagaa
    44041 tgatgtattc gcgccgtaac aggcctaaag gaagcggcct gccagtcggt tggaaatgat
    44101 cgggtaatgg cagttgcaat gctggcggtg ctactgacgc catcaccatc gcccagactt
    44161 ttgccagcac aaaatccccg ccggtggtaa tgacctcttc gccgagatca ataaaataga
    44221 taacaccttg attatccagc gcacaccatg ggcagtcagc caaatgaccg ggataaacat
    44281 gcattgccga aacggtacat ttctttaact gttggcgtag agaatccagt gctgataccc
    44341 acgctttagc cgtcggacgc ccggttgcca cgccactttc cgtaaacgcc tgctgaaaca
    44401 tggcttcaac atcactcggt aacatcgata gcggaatcga tcgtggcggc ggttttaaac
    44461 cacgtcgctg attatctgac gcgtaggcat aacggaaatg ggtaatatcc gtctccagcg
    44521 cattacccgc atcagagata agcggcacac cagaataagg atgccggcca ccaaacaaga
    44581 cgtgaaaaat cagcaacgca aggccaaaat tatcgtgatt ttctgtgcgt tcaaagccga
    44641 caaatgacga caaggtttgt agctctggcg gcgtaaaatg cgacacgccg acttcgcata
    44701 aatgcagtgt gccattggcg ttaatctgaa aggagtcgct atcaatcaac accactttgc
    44761 tgtcgcgacc taccataaaa ctgttctggt ttacgtcacc cacgacgtgc ccgtgctcgt
    44821 gaaccgtagc aaaagatgaa gcaatattgc gcgcaacata gagtagaaaa tcccacgcac
    44881 aatgagggta acgttgacga cgatgtgccg ggctatagat catatgaatc ggttctttac
    44941 cagaaacttt tggcatcata aaaccgataa cttttccgcc tcgtccaccg tgaagcgttg
    45001 cctgcggcca ggcgacataa ttcaacaatt gcgcgtcagc tgtcgcagcc ataaaggcaa
    45061 gtttgtcctg ttttaaggcg ggtggcggcg tgtgataaat cttggcgacg ctatcgacaa
    45121 actcattgat atcataaacc gcgccttcac cgcctttgcc cagttcacgg cctggcgtaa
    45181 cgcactcacc agtagcagta tataaagtgg gtttcatggt aaccacagcg ccagtgccaa
    45241 cgttttatca tcatccgtac gctcgtttac cgccgggctg gacaaaaact gctttaacaa
    45301 ttcaggtaat aaatcgagtt gttcctgcgt tgctgacgcc agtccattga aaaacggggt
    45361 aaagaaaggc acatgaggag aattatccag catatttaac gccagccgct gaataccatc
    45421 cgtaaatgct gcaactttat gcgcacgccc agtgctggtg aaagtttcca gtctggaaac
    45481 ggcatcttcg tcggtaatga agtgcgtcat gttggcatat tcacccacca tcggcgtgag
    45541 aggcaattgc agaccgtgac ccaaatctac caccacgccg ccatcgccaa tctgcataat
    45601 caaggtgccg tcaggcgatg aaatcaatcc cagaaacgtg caggcaaaat cgcgaactgc
    45661 taatgctttg gcttctgcct cggcaaaaag tcgttgatga atagtcagta ccatatttgt
    45721 ggcgaggaca tcattaagcc ccaattcacc gtcctgtact ttttgcacca taaacgccat
    45781 cgcttcattg acggcgagcg tcgcgccttc tcctccctgc gaaacgctac ctgcgccatc
    45841 agcaaggaac atcaccagta atggttgctg gtcgtttaac cagacaacct gcatctggca
    45901 ggcatcctga caaggcaaat cagcactaat gtgcgatgtt ccaacagcgg atgcataaac
    45961 cagacgccag ctcacactga cgtccagcct tttggcgctt ccagcacaac ttccgttccc
    46021 ggcgtggagc gggaaaccga acgcagcgaa ctggataacc agctaaacag ttcacggaat
    46081 tgtaatccct gcagtggcaa aggctgacga acgctgattt gtgccagagt cttcatatcc
    46141 gccccctgaa caccgatgga aaagaaggca aacttcttat cttcttcccc ctgaaacact
    46201 ttgttggcgg cagcctgcca ctcatcggtt ggagcgccat cggtgatcag gaaaatccac
    46261 gggcgataat acgaaatacc atttgcgcgg tactcacgtt tcctctcttc aaccatatcc
    46321 agggctttgg taatggcggc ccccataggc gtatccccct gggcaaacag gatgggcggg
    46381 aaaaagttag ctgcgctggt aaagggttgt tccacatgca ccgggccgaa cgtcacaatg
    46441 ccgagttcca ctctttttaa agccagcgga tcagcaagca gttcatcacg aaaggtaacc
    46501 aatccggcgt taagttcatt aataggtctg ccgctcatcg agccagaaac atccagcagc
    46561 aaaatgcagg ggcaacgtgg ttccgggtta ctggcaaaat cgctggtggc aaacgtgatt
    46621 tgttcgctca taagaaatcc tttcttagag aattatcgag gtccggtatc ataccgattg
    46681 caataatgaa ataagagcaa acgaagaaac tttcagaatt aggcccgtcg gcgcagaaaa
    46741 ttttgctatt cgcctcgcaa gaacggagag ccgtcgattg acactaaggg cggagtgaca
    46801 taatttcagg agtgagggtt agggagaggt ttccccctcc ccctggtgtt cttagtaagc
    46861 ctggaagcta atcactaaga gtatcaccag tatgatgacg tgcttcatca taaccctttc
    46921 cttattaaaa gccctcttct ccgggagagg gtttcccgtt tcagcgtccc gctgaaatcg
    46981 tcggcttacc tcctttcgcc ctgaatgcag tctatcgcta acgcgtgaac aaaatagttt
    47041 catctgtgat tattgcgtaa cgactcgcaa agaatatttg ctggcaggat cgcagactac
    47101 aaagcctgcg gattgacaat cttatcgtga aggcataatt tcaggagtga gggtagagcg
    47161 gggtttcccc cgccctggta gtcttagtaa gcggggaagc ttatgactaa gagcaccacg
    47221 atgatgagta gcttcatcat gaccctttcc ttattaaggc cccttcctcg ggaggggctt
    47281 tcccgtttca gcgtcccgct gaaatcgtcg gcttacctcc tttcgccctg tatgcagtct
    47341 atcgctgact tgcggataaa atagtttcca gtgtgattat tgcgtaacgt ctcgcaaaga
    47401 atatttgctg gcaggatcgc agactgcaaa gcctgcagat tgacaatctt atcgtgaagg
    47461 catactttca ggagtgaggg tagagcgggg tttcccccgc cctggtagcc ttagtaagct
    47521 ggccagctaa tgactaagag caccacgatg atgagtagct tcatcatgac cctttcctta
    47581 tttatggccc cttcctcggg aggggctttc ccgtttcagc gtcccgctga aatcggcggc
    47641 ttacctcctt tcgccatgca agcagtctat cgctaacgcg tagataaaat agtttcctgt
    47701 gttattactg gatgcgtgct cgcaaatgtg cccgtcattc agacgattcc cgacagtgtt
    47761 tcataattcc tccatttttc tcccttattg gctggctaca ctagtatcat tccgcgaaac
    47821 gtttcaggaa gagaaactct taacgatgaa aggcagttat aaatcccgtt gggtaatcgt
    47881 ggtggttatc gccgccatcg ccgcattctg gttctggcaa ggccgcaatg actcccagag
    47941 tgcagcccca ggggcgacga aacaagcgca gcaatcgcca gcgggtggtc gccgtggtat
    48001 gcgttccggc ccattagccc cggttcaggc ggcgaccgcc gtagaacagg cagttccgcg
    48061 ttacctcacc gggcttggca ccattatcgc cgctaatact gttacggtgc gcagccgcgt
    48121 ggacggtcaa ctgatggcgt tacatttcca ggaaggccag caggtcaaag caggcgattt
    48181 actggcagaa attgacccca gccagttcaa agttgcatta gcacaaaccc agggccaact
    48241 ggcaaaagat aaagccacgc ttgctaacgc ccgccgtgat ctggcgcgtt atcagcaact
    48301 ggcaaaaacc aatctcgtct cccgtcagga gctggatgcc caacaggcgc tggtcagtga
    48361 aaccgaaggc accattaagg ctgatgaagc aagcgtcgcc agcgcgcagt tgcaactcga
    48421 ctggagccgc atcaccgcac cagtcgatgg tcgcgttggt ctcaagcagg ttgatgttgg
    48481 taaccaaatc tccagtggtg ataccaccgg aattgtggtg atcacccaga cgcatcctat
    48541 cgatttagtc tttaccctgc cggaaagcga tatcgctact gtagtgcagg cgcaaaaagc
    48601 cggaaaaccg ctggtggtag aagcctggga tcgcaccaat tcgaagaaat taagtgaagg
    48661 cacgctgtta agcctcgata accaaatcga tgccacaacc ggtacgatta aagtgaaagc
    48721 acgctttaat aatcaggatg atgcgctgtt tcccaatcag tttgttaacg cgcgcatgtt
    48781 agtcgacacc gaacaaaacg ccgtagtgat cccaacagcc gccctgcaaa tgggcaatga
    48841 aggccatttt gtctgggtgc tgaatagcga aaacaaggtc agcaaacatc tggtgacgcc
    48901 gggcattcag gacagtcaga aagtggtgat ccgtgcaggt atttctgcgg gcgatcgcgt
    48961 ggtgacagac ggcattgatc gcctgaccga aggggcgaaa gtggaagtgg tggaagccca
    49021 gagcgccacc actccggaag agaaagccac cagccgcgaa tacgcgaaaa aaggagcacg
    49081 ctcctgatgc aggtgttacc cccgagcagc acaggcggcc cgtcgcgcct gtttattatg
    49141 cgtcctgtcg ccaccacgct gctgatggtg gcgatcttac tcgccgggat tatcggttat
    49201 cgcgccctgc ccgtttcggc gctgccggaa gtggactatc cgaccattca ggtggtcacg
    49261 ctctacccag gtgccagccc ggatgtcatg acctctgccg ttaccgcgcc gctcgaacgc
    49321 cagttcgggc agatgtctgg cctgaaacag atgtcgtcgc aaagttccgg cggtgcgtca
    49381 gttatcactt tgcagttcca gctaacatta ccgctcgatg tcgccgagca ggaagtgcag
    49441 gccgcgatta acgctgcgac caacttgttg ccgagcgatc tgcctaaccc gccggtttac
    49501 agcaaagtga acccggcaga tccgccgatc atgacgctcg ccgtcacctc aaccgccatg
    49561 ccgatgacgc aagtggaaga tatggtggaa acccgcgtcg cgcagaaaat ctcgcagatt
    49621 tccggcgtcg gcctggtgac gctttccggc ggtcagcgtc cggctgttcg cgtcaaactt
    49681 aacgctcagg cgattgccgc cctcggcctg accagcgaaa ccgtgcgcac cgccattacc
    49741 ggcgctaacg ttaactcggc aaaaggtagc ctcgacggcc cttcccgtgc agtcacgctt
    49801 tccgcgaacg accagatgca atccgccgaa gagtatcgcc agctaatcat cgcctaccag
    49861 aacggcgcgc caattcgtct gggcgatgtc gcaactgtag agcaaggtgc agaaaacagc
    49921 tggctcggcg cgtgggcgaa caaagaacag gccattgtga tgaatgttca gcgccagccc
    49981 ggtgctaaca ttatctccac cgccgacagc attcggcaga tgctgccaca gctcactgag
    50041 agtctgccga aatcggtgaa ggtgacagtg ctttccgatc gcaccaccaa tatccgcgca
    50101 tccgtcgatg atactcagtt tgaattgatg atggctatcg cgctggtagt catgattatc
    50161 tacctgtttt tgcgcaatat tccggcgacc atcattcccg gtgttgctgt accgctgtcg
    50221 ttaatcggca ctttcgcggt tatggtgttt ctcgattttt caatcaataa cctgacactg
    50281 atggcgttaa ctatcgccac cggattcgtg gtcgatgacg ccatcgtggt gatcgaaaac
    50341 atttcccgct atatcgaaaa aggcgaaaaa ccgttggcgg cggcgctcaa gggcgcaggt
    50401 gaaatcggct ttaccattat ctcgctgacc ttctcactga ttgcggtgtt gatcccactg
    50461 ctgtttatgg gcgatatcgt cgggcgactg ttccgcgaat ttgctattac cctggcggta
    50521 gcgattttga tctcagcggt ggtgtcgctg accctgacac cgatgatgtg cgcgcggatg
    50581 ctcagccagg agtcgttgcg taaacagaac cgcttctccc gtgcctcgga aaaaatgttc
    50641 gacaggataa tcgccgccta tggtcgtgga ctggcgaaag tgctgaatca tccgtggctg
    50701 accttaagcg tggcactcag cacgctgctg cttagcgtgc tgctgtgggt gttcattccg
    50761 aaaggtttct tcccggtaca ggacaatggc attattcagg gcactttgca ggcaccgcaa
    50821 tccagctcct ttaccaatat ggcccagcga caacgccagg tcgcggacgt gattttgcag
    50881 gatccggcag tgcaaagcct gacctcattt gttggcgttg atggcactaa cccgtcgctg
    50941 aatagtgcac gtttacaaat caacctcaaa ccgttggatg aacgtgatga tcgggtgcaa
    51001 aaagtcatcg cccgtctgca aacggcggta gataaagtgc cgggcgtcga tctcttcctg
    51061 caaccaacgc aggatctgac tattgatact caggtcagcc gcacccagta ccagtttacc
    51121 ttgcaggcca cctcactgga tgcgctcagt acctgggtgc cacagttgat ggaaaaactc
    51181 cagcaactgc cacagctttc tgatgtctcc agcgactggc aggaccaagg gctggtggcg
    51241 tatgtcaatg ttgatcgcga cagcgccagc cgtctgggga tcagcatggc ggatgtcgat
    51301 aacgccctgt acaacgcgtt tggtcagcga ctgatttcca ctatttatac tcaggctaac
    51361 cagtatcgcg tagtgctgga gcacaacacc gaaattaccc cgggcctcgc ggcgctggat
    51421 accattcgcc tgaccagtag cgacggcggc gtggtgccgt taagctcaat tgccaaagtt
    51481 gaacagcgtt ttgcgccgct ttccatcaac catctggatc agttcccggt aacgaccatc
    51541 tcctttaacg tgccggataa ctattcgctg ggcgatgcgg tgcaggagat tatggacacc
    51601 gaaaagacgc tgaatctgcc ggtggatatc accacgcaat tccagggcag caccctcgcc
    51661 ttccagtcgg cgctgggcag cactgtctgg ctgattgtcg cggcggtggt ggcgatgtat
    51721 atcgtgctcg gcattctgta cgagagcttt attcacccga tcaccattct ctcgacgcta
    51781 cccaccgcag gggtcggcgc gctgctggca ttaatgattg ccggtagcga actggatgtg
    51841 attgcgatta tcggcattat tttgctgatc ggtattgtga agaagaacgc catcatgatg
    51901 atcgacttcg cgctggctgc tgagcgcgag caaggcatgt cgccgcgcga tgcaatctac
    51961 caggcttgtc tgttgcgttt tcgtccgatc ctgatgacca ccctggcggc tctgcttggc
    52021 gcgctgccgc tgatgttgag taccggggtc ggcgcggaac tgcgtcgtcc gttaggtatc
    52081 ggcatggtcg gcggtctgat tgtcagccag gtgctgacgc tgttcaccac gccggtgatt
    52141 tatctgctgt tcgaccgcct ggcattgtgg accaaaagtc gctttgctcg ccatgaagag
    52201 gaggcgtaag tgaagttttt tgccctcttc atttaccgcc cggtggcgac gattttactg
    52261 tcggtcgcca ttaccctgtg cggcatactg ggcttccgta tgctgccggt cgccccgctg
    52321 ccgcaggtcg attttccggt gattatggtc agcgcctcgc tgcccggtgc gtcaccagaa
    52381 acaatggcgt cttccgttgc cacgccgctg gagcgctcgc ttgggcgcat tgccggggtc
    52441 agcgaaatga cctccagcag ttcgctcggc agcacacgta ttattttgca gtttgatttt
    52501 gaccgggaca tcaacggcgc agcgcgtgat gtgcaggcgg cgatcaacgc tgcacaaagt
    52561 ttgctgccca gtgggatgcc cagccgtccg acctatcgca aagcgaaccc gtcggatgcg
    52621 ccaattatga tcctcacgct gacatccgat acttattcgc agggtgaact gtacgatttc
    52681 gcctcgacgc agctggctcc gacgatttcg caaatcgacg gtgttggtga tgtcgatgtc
    52741 ggaggcagct cactgcccgc cgtacgcgtc gggctgaatc cgcaggcgct gtttaatcag
    52801 ggcgtgtcgc tggacgacgt acgcaccgcc atcagcaatg ccaacgtgcg taaaccgcag
    52861 ggcgcgctgg aagatggcac tcaccgctgg cagatccaga ccaatgatga gctaaaaacc
    52921 gccgctgaat atcagccgtt gattattcac tacaacaacg gcggcgcggt tcgtctgggc
    52981 gatgtggcga cggtgaccga ctcagtgcag gatgtgcgca acgccgggat gaccaacgcc
    53041 aaaccggcta ttttactgat gatccgcaaa ctgccggaag ccaatattat ccagacggtt
    53101 gacagcatcc gggcaaaatt accggagttg caggaaacca ttccggcggc gattgatctg
    53161 caaattgccc aggatcgctc ccccaccatt cgcgcctcgc tggaagaagt cgagcaaacg
    53221 ctgattatct cggtggcgct ggtgattctg gtggtgtttt tattcctgcg ctcgggtcgc
    53281 gccactatta ttcccgccgt ttcggtgccg gtttcgctga ttggtacgtt tgcggcgatg
    53341 tacctgtgcg gattcagtct caataacctt tcgttaatgg cgctcaccat cgctactggt
    53401 ttcgtggtgg atgacgccat cgtggtgctg gaaaacattg cacgtcatct ggaagcggga
    53461 atgaaaccgt tgcaagccgc actgcaaggt actcgcgaag tcggttttac ggtgctgtcg
    53521 atgagtctgt cactggtggc ggtgttcctg ccgctgctgt tgatgggcgg attgccgggc
    53581 cgactgttac gcgagtttgc cgtgacgctt tctgtcgcca ttggtatttc gttgctggtt
    53641 tctctgacat taacgccaat gatgtgtggc tggatgctga aagccagcaa gccgcgcgag
    53701 caaaagcgac tgcgtggttt tggtcgcatg ttggtagccc tgcaacaagg ctacggcaag
    53761 tcactgaaat gggtgctcaa tcatacccgt ctggtgggcg tggtgctgct tggcaccatt
    53821 gcgctgaata tctggctgta tatctcgatc ccgaaaacct tcttcccgga gcaggacact
    53881 ggcgtgttga tgggcgggat tcaggcggat cagagtattt cgtttcaggc gatgcgcggt
    53941 aagttgcagg atttcatgaa aattatccgt gacgatccgg cagtggataa tgtcaccggc
    54001 tttacaggcg gttcgcgagt gaacagcggg atgatgttta tcaccctcaa gccacgcgac
    54061 gaacgcagcg aaacggcgca gcaaattatc gaccgtctgc gcgtaaaact ggcgaaagaa
    54121 ccgggggcga atctgttcct gatggcggta caggatattc gcgttggtgg gcgtcagtcg
    54181 aacgccagct accagtacac gttgttatcc gacgacctgg cggcactgcg cgaatgggag
    54241 ccgaaaatcc gcaaaaaact ggcgacgttg ccggaactgg cggacgtgaa ctccgatcag
    54301 caggataacg gcgcggagat gaatctggtt tacgaccgcg acaccatggc acggctggga
    54361 atcgacgtgc aggccgccaa cagcctgtta aataacgcct tcggtcagcg gcaaatctcg
    54421 accatttacc agccgatgaa ccagtataaa gtggtgatgg aagtggatcc gcgctatacc
    54481 caggacatca gtgcgctgga aaaaatgttc gttatcaata acgaaggcaa agcgatcccg
    54541 ctgtcgtatt tcgctaaatg gcaaccggcg aacgctccgc tgtcggtgaa tcatcaggga
    54601 ttatcggcgg cctcgaccat ctcgttcaac ctgccgaccg gaaaatcgct ctcggacgcc
    54661 agtgcggcga tcgatcgcgc aatgacccag cttggtgtgc cttcgacggt gcgcggcagt
    54721 tttgccggaa cagcgcaggt gttccaggag acgatgaact cacaggtgat cctgattatc
    54781 gccgccatcg ccacggtgta tatcgtgctg ggtatccttt acgagagtta cgtacaccca
    54841 ctgacgattc tctccaccct gccctcggcg ggcgttggtg cgctgctggc gctggagctg
    54901 ttcaatgccc cgttcagcct aatagccctg atagggatca tgctattaat cggcatcgta
    54961 aagaaaaacg ccattatgat ggtcgatttt gcgctcgaag cccaacggca cggtaacctg
    55021 acgccgcagg aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgact
    55081 accctggcgg cgctgtttgg cgcgctgccg ctggtgttgt cgggcggtga cggctcggag
    55141 ctgcggcacc ccctggggat caccattgtc ggtggactgg taatgagcca gcttctgacg
    55201 ctgtatacca cgccggtggt gtatctcttt ttcgaccgtc tgcggctgcg tttttcgcgt
    55261 aaacctaaac aaacggtaac cgagtaaatg acagaacttc ccgacagcac ccgctggcaa
    55321 ttgtggattg tggctttcgg cttctttatg cagtcgctgg acaccaccat cgttaacacc
    55381 gcccttccct caatggcgca aagcctcggg gaaagtccgt tgcatatgca catggtcatt
    55441 gtctcttatg tgctgaccgt ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa
    55501 gtcggcgtgc gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt
    55561 tgcgcgcttt ccggcacgct gaacgaactg ttgctggcac gcgcgttaca gggcgttggc
    55621 ggtgcgatga tggtgccggt cggcaggttg acggtgatga aaatcgtacc gcgcgagcaa
    55681 tatatggcgg cgatgacctt tgtcacgtta cccggtcagg tcggtccgct gctcggtccg
    55741 gcgctcggcg gtctgctggt ggagtacgcg tcgtggcact ggatcttttt gatcaacatt
    55801 ccggtgggga ttatcggtgc gatcgccaca ttgctgttaa tgccgaacta caccatgcag
    55861 acgcggcgct ttgatctttc cggcttttta ttgctggcgg ttggcatggc tgtattaacc
    55921 ctggcgctgg acggcagtaa aggtacaggt ttatcgccgc tggcgattgc tggcctggtc
    55981 gcagttggcg tggtggcact ggtgctttat ctgctgcacg ccagaaataa caaccgcgcc
    56041 ctgttcagtc tgaaactgtt ccgcactcgt accttttcgc tgggcctggc ggggagcttt
    56101 gccggacgta ttggcagtgg catgttgccc tttatgacac cggttttcct gcaaattggc
    56161 ctcggtttct cgccgtttca tgccggactg atgatgatcc cgatggtact tggcagcatg
    56221 ggaatgaagc gaattgtggt tcaggtggtg aatcgctttg gttatcgtcg ggtactggta
    56281 gcgaccacgc tgggtctgtc gctgatcacc ctgttgttta tgactaccgc tctgttgggc
    56341 tggtactacg ttttgccgtt cgtcctgttt ttacaaggga tggtcaactc gacccgtttc
    56401 tcctccatga acaccctgac gctgaaagat ctcccggaca atctggcgag cagcggcaac
    56461 agtctgctgt cgatgattat gcaattgtcg atgagtatcg gcgtcaccat cgccgggctg
    56521 ttgctgggac tttttggttc gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc
    56581 tttatgtaca cctggcttag catggcgttt atcatcgccc ttccggcgtt catctttgcc
    56641 agagtgccga acgatacgca tcaaaatgta gctatttcgc ggcgaaaaag gagcgcgcaa
    56701 tgaagttctg gcgacctggt attaccggca aactgtttct ggcgattttc gccacctgca
    56761 ttgttttgct gatcagtatg cactgggcgg tgcgtatcag ttttgagcgt ggctttattg
    56821 attacatcaa gcatggtaat gaacagcgat tacaactgtt aagtgatgcg cttggcgagc
    56881 agtatgcgca gcatggcaac tggcgcttcc tgcgcaacaa tgatcgcttt gtctttcaga
    56941 tcctgcgttc atttgaacac gataattcgg aagataaacc cggcccgggt atgccaccgc
    57001 acggctggcg tacccagttc tgggtggttg atcaaaacaa caaagtgctg gttggtccgc
    57061 gagcgccgat tccacctgac ggtacacggc gacccattct ggtcaacggt gcggaagttg
    57121 gcgcggtgat cgcctccccc gttgagcggt taacgcgcaa tactgatatc aatttcgata
    57181 aacaacagcg gcaaaccagc tggttgattg tcgccctggc aacgttactc gcggcacttg
    57241 ccacttttct gctggcgcgc ggtttactgg caccggtaaa acgacttgtc gatggcacgc
    57301 acaaactggc ggcgggcgat ttcactaccc gcgtaacgcc caccagtgaa gatgaactgg
    57361 gcaaactggc gcaagacttc aaccagcttg ccagcacact ggagaaaaac cagcaaatgc
    57421 ggcgcgattt tatggccgat atttctcacg aactgcgtac gccattagcg gtgctgcgcg
    57481 gtgaactgga agctattcag gatggcgtgc gcaaattcac gccggagacg gtggcgtctt
    57541 tacaggcgga ggtcggtaca ctgaccaaac tggttgacga tctccatcag ttgtcgatgt
    57601 ctgatgaagg cgccctcgcc tatcaaaaag caccggtaga tttgatccca ctgctggaag
    57661 tggcgggcgg cgcatttcgc gaacgattcg ccagccgtgg cctgaaactg caattttccc
    57721 tgccagacag tattaccgta tttggcgatc gcgaccgttt aatgcagtta ttcaataatt
    57781 tacttgaaaa cagcctgcgc tacactgaca gcggcggcag cctgaaaatc tctgccgagc
    57841 agcacgacaa aacggtgcgc ctgacctttg ccgacagtgc gccaggtgtc agtgacgagc
    57901 agctacaaaa attatttgaa cgtttttatc gcaccgaagg ttcccgcaac cgcgccagcg
    57961 gtggttccgg gctggggctg gcgatttgcc tgaacattgt tgaagcacat aatggccgca
    58021 ttattgctgc ccattcgcct tttggcgggg taagcattac agtagagtta ccgctggaac
    58081 gggatttaca gagagaagta tgaccgagtt accaatcgac gaaaacacac cgcgtatttt
    58141 gatcgtggaa gatgaaccga agctggggca gttgctcatt gattatctgc gtgctgcgag
    58201 ctatgcgccg acgcttatca gccacggcga tcaggtactg ccgtatgtgc gccagacacc
    58261 accggatctg atcctgttgg atctgatgct ccctggcacc gatggcttga cgctgtgccg
    58321 ggaaattcgt cgtttttctg acattcctat cgtgatggtg acggcaaaaa tcgaagagat
    58381 cgaccgcctg ctcggactgg agattggcgc agatgactac atctgcaagc cgtacagccc
    58441 acgggaagtg gtagcgcgcg tcaaaaccat tttgcgccgt tgcaaaccgc agcgcgaatt
    58501 gcagcaacag gatgccgaaa gcccgttgat tatcgacgaa ggtcgttttc aggcttcatg
    58561 gcgcggtaaa atgcttgacc tgacgcctgc ggaatttcgt ctgttgaaaa cgctctctca
    58621 tgaaccagga aaagtgttct cccgcgagca attgctcaat catctttatg acgactaccg
    58681 cgtagtaacc gaccgcacca ttgacagcca cattaaaaac ctgcgccgca agctggaatc
    58741 tctcgacgcc gaacagtcat ttatccgcgc cgtttacggc gttggttacc gctgggaagc
    58801 cgacgcctgc cgcatcgttt agttttagcg acatgacttt gttagccgga gatggcgttc
    58861 agaccagatc cggcaacatt atcccacgca tggtcagcaa actga
//
LOCUS       DAZPBM010000006        54953 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPBM010000006 DAZPBM010000000
VERSION     DAZPBM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.006"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHUZQ_000301"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHUZQ_000301"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1024109.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHUZQ_000302"
     CDS             complement(1672..2667)
                     /locus_tag="ACHUZQ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1024110.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHUZQ_000303"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHUZQ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUZQ_000304"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUZQ_000304"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1024111.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUZQ_000305"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUZQ_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1024112.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUZQ_000306"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUZQ_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1024113.1"
                     /translation="MEQINSNKKQSSRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUZQ_000307"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUZQ_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1024114.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUZQ_000308"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUZQ_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1024115.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUZQ_000309"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUZQ_000309"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1024116.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUZQ_000310"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUZQ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1024117.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUZQ_000311"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUZQ_000311"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1024118.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUZQ_000312"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUZQ_000312"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1024119.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHUZQ_000313"
     CDS             complement(17699..18322)
                     /locus_tag="ACHUZQ_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1024120.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHUZQ_000314"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHUZQ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR1024121.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACHUZQ_000315"
     CDS             complement(18971..19213)
                     /locus_tag="ACHUZQ_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1024122.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHUZQ_000316"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHUZQ_000316"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1024123.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHUZQ_000317"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHUZQ_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1024124.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHUZQ_000318"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHUZQ_000318"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1024125.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22593..24290
                     /gene="ypdA"
                     /locus_tag="ACHUZQ_000319"
     CDS             22593..24290
                     /gene="ypdA"
                     /locus_tag="ACHUZQ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR1024126.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24305..25039
                     /gene="ypdB"
                     /locus_tag="ACHUZQ_000320"
     CDS             24305..25039
                     /gene="ypdB"
                     /locus_tag="ACHUZQ_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1024127.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25052..25909
                     /locus_tag="ACHUZQ_000321"
     CDS             25052..25909
                     /locus_tag="ACHUZQ_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1024128.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="ACHUZQ_000322"
                     /pseudo
     CDS             complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="ACHUZQ_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="ACHUZQ_000323"
     CDS             complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="ACHUZQ_000323"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1024129.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="ACHUZQ_000324"
                     /pseudo
     CDS             complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="ACHUZQ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30569..31816)
                     /locus_tag="ACHUZQ_000325"
     CDS             complement(30569..31816)
                     /locus_tag="ACHUZQ_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR1024130.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31838..32164)
                     /locus_tag="ACHUZQ_000326"
     CDS             complement(31838..32164)
                     /locus_tag="ACHUZQ_000326"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1024131.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32384..33349)
                     /gene="glk"
                     /locus_tag="ACHUZQ_000327"
     CDS             complement(32384..33349)
                     /gene="glk"
                     /locus_tag="ACHUZQ_000327"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1024132.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            33553..34809
                     /locus_tag="ACHUZQ_000328"
     CDS             33553..34809
                     /locus_tag="ACHUZQ_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1024133.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34924..35250
                     /gene="ypeC"
                     /locus_tag="ACHUZQ_000329"
     CDS             34924..35250
                     /gene="ypeC"
                     /locus_tag="ACHUZQ_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1024134.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35391..36629)
                     /locus_tag="ACHUZQ_000330"
     CDS             complement(35391..36629)
                     /locus_tag="ACHUZQ_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1024135.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36965..38167
                     /gene="nupC"
                     /locus_tag="ACHUZQ_000331"
     CDS             36965..38167
                     /gene="nupC"
                     /locus_tag="ACHUZQ_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1024136.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38217..40405)
                     /locus_tag="ACHUZQ_000332"
                     /pseudo
     CDS             complement(38217..40405)
                     /locus_tag="ACHUZQ_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(40614..40689)
                     /locus_tag="ACHUZQ_000333"
     tRNA            complement(40614..40689)
                     /locus_tag="ACHUZQ_000333"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40654..40656),aa:Ala,seq:ggc)
     gene            complement(40729..40804)
                     /locus_tag="ACHUZQ_000334"
     tRNA            complement(40729..40804)
                     /locus_tag="ACHUZQ_000334"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40769..40771),aa:Ala,seq:ggc)
     gene            41025..41384
                     /locus_tag="ACHUZQ_000335"
     CDS             41025..41384
                     /locus_tag="ACHUZQ_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1024137.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41386..41778
                     /locus_tag="ACHUZQ_000336"
     CDS             41386..41778
                     /locus_tag="ACHUZQ_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1024138.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="ACHUZQ_000337"
     CDS             complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="ACHUZQ_000337"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1024139.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43504..43579
                     /locus_tag="ACHUZQ_000338"
     tRNA            43504..43579
                     /locus_tag="ACHUZQ_000338"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43537..43539,aa:Val,seq:tac)
     gene            43624..43699
                     /locus_tag="ACHUZQ_000339"
     tRNA            43624..43699
                     /locus_tag="ACHUZQ_000339"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43657..43659,aa:Val,seq:tac)
     gene            43746..43821
                     /locus_tag="ACHUZQ_000340"
     tRNA            43746..43821
                     /locus_tag="ACHUZQ_000340"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43779..43781,aa:Val,seq:tac)
     gene            43826..43901
                     /locus_tag="ACHUZQ_000341"
     tRNA            43826..43901
                     /locus_tag="ACHUZQ_000341"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43859..43861,aa:Lys,seq:ttt)
     gene            43932..44057
                     /locus_tag="ACHUZQ_000342"
     ncRNA           43932..44057
                     /ncRNA_class="other"
                     /locus_tag="ACHUZQ_000342"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            44177..44518
                     /locus_tag="ACHUZQ_000343"
     CDS             44177..44518
                     /locus_tag="ACHUZQ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1024140.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(44509..45435)
                     /locus_tag="ACHUZQ_000344"
     CDS             complement(44509..45435)
                     /locus_tag="ACHUZQ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1024141.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            45525..46523
                     /locus_tag="ACHUZQ_000345"
     CDS             45525..46523
                     /locus_tag="ACHUZQ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1024142.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46520..46738)
                     /locus_tag="ACHUZQ_000346"
     CDS             complement(46520..46738)
                     /locus_tag="ACHUZQ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1024143.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46740..48755)
                     /gene="ligA"
                     /locus_tag="ACHUZQ_000347"
     CDS             complement(46740..48755)
                     /gene="ligA"
                     /locus_tag="ACHUZQ_000347"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1024144.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48826..49812)
                     /gene="zipA"
                     /locus_tag="ACHUZQ_000348"
     CDS             complement(48826..49812)
                     /gene="zipA"
                     /locus_tag="ACHUZQ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1024145.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            50042..50803
                     /gene="cysZ"
                     /locus_tag="ACHUZQ_000349"
     CDS             50042..50803
                     /gene="cysZ"
                     /locus_tag="ACHUZQ_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1024146.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50988..51959
                     /gene="cysK"
                     /locus_tag="ACHUZQ_000350"
     CDS             50988..51959
                     /gene="cysK"
                     /locus_tag="ACHUZQ_000350"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1024147.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52343..52600
                     /gene="ptsH"
                     /locus_tag="ACHUZQ_000351"
     CDS             52343..52600
                     /gene="ptsH"
                     /locus_tag="ACHUZQ_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1024148.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52645..54372
                     /gene="ptsI"
                     /locus_tag="ACHUZQ_000352"
     CDS             52645..54372
                     /gene="ptsI"
                     /locus_tag="ACHUZQ_000352"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1024149.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54413..54922
                     /gene="crr"
                     /locus_tag="ACHUZQ_000353"
     CDS             54413..54922
                     /gene="crr"
                     /locus_tag="ACHUZQ_000353"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1024150.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT        14332 a        13804 c        12950 g        13867 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgctag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aataactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22441 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22501 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22561 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22621 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22681 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22741 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22801 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22861 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22921 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22981 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga
    23041 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    23101 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc
    23161 tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
    23221 tggatatcgc caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca
    23281 aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg
    23341 atcatgtgct ggcctatgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca
    23401 ttagcccgac tacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg
    23461 aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg gagaaagggg
    23521 tcgtgaccgg aacgctgaaa atttactact gccacgcgca tcagatcacc tcgtcattac
    23581 aggaaatggc ggtcggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg
    23641 agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
    23701 cccattttct gtttaacgct ctgaacgcta tttcatcgtc aatccgtctg aatccggata
    23761 ccgctcgcca gttgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
    23821 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
    23881 agcaggcccg ctttggtgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt
    23941 gctgcattcc cagcctgctg atccagccgt tggtggagaa cgccattgtc cacggtattc
    24001 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcgga aatcgggtac
    24061 gcattgcggt gcgagatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
    24121 cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat
    24181 tgtatggcga ggggctgcat atccgccgcc tggagccggg gacggaaatt gcgttttaca
    24241 ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga gccggagtga
    24301 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
    24361 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
    24421 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
    24481 ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
    24541 catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
    24601 tctcaaaccg tatcaggagt cacgtattac cgggatgctg caaaaactgg aagcggcctg
    24661 gcaacaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga atgacaccat
    24721 taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga
    24781 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
    24841 cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt
    24901 ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca cctacattct
    24961 gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga aagaatttcg
    25021 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
    25081 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
    25141 ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg attttccccg
    25201 tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc agcagcgaat
    25261 ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga tcaacaacaa
    25321 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
    25381 tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca gcctgcaacg
    25441 cggtgaaggt gaagcgatgc ttaccgccct cacccatctt agccgttcgc cgctggagca
    25501 gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg tggtgaatat
    25561 gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt gtaactgggt
    25621 tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac
    25681 gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta tcgagtatgt
    25741 gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc tgtcaattca
    25801 tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg ttttccggcg
    25861 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
    25921 taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa acaacgactg
    25981 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
    26041 cccttcgtta gcacccagcg tcagcatgat caccagttca acttcgccca tttctgactg
    26101 ccagtcaacc ggtttcgcca gccgggcaat gctgatactg gaatgacgga tccactgaga
    26161 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct cttcccgctg
    26221 ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc cgaggttgcc
    26281 gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa agatattctc
    26341 cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat
    26401 ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
    26461 cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac tcagctcatc
    26521 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
    26581 gcctacccat ttgccccgct gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa
    26641 cgatggcgta atcgggttat atagcggcga tacgcgcggg ttattacgat cgaccgcata
    26701 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cggagaagtg
    26761 gtcgatgatg tagcacaccg acggcacttc caccatgatc ccaagcgtaa tcgtctctgc
    26821 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ctttcaccca
    26881 taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc cgaaactggc
    26941 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaatacg
    27001 taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat aaggaatgct
    27061 tttatcgccg ccgatgtcca tcgtgcggaa gataatcggc ttgtcgcccg ccgccagtag
    27121 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcgc gatccatata
    27181 gagcatttcg gtacggaaca gcccgacacc ttccgcgccg ttggcaaacg cgcctggcgc
    27241 ttccagagcg gtgccgatat tcgccgcaat atcaatacgc ttgttatcac gggaataggc
    27301 cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg tctgcgcgac
    27361 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc
    27421 aagcactgca ggttgcccgg cataacgggc aatcgcatcc agcggcaagc cactcagtac
    27481 cgggatcgcc gaggcacggg ccagaatcag tgtatgcgag gtgcgcccgg ttttctccag
    27541 aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg ttaaatcttc
    27601 agccaccaga atggtcggtt tttccagcac cagattgttg cgcggcttca gttccggcta
    27661 ggtgatatgc agcaactgtt cgctgatatc gcgaatgtcg ctgacccgct cgcgcagata
    27721 atcgctggca gaagcagata gtttggcgca aacctgctcc atattgctga tgatcgccgc
    27781 ccccagtccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa attcatcatc
    27841 ctgaatcagc gacaaatgag cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
    27901 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
    27961 cgcactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca gggtcagcgt
    28021 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt taaggcggct
    28081 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtcggctgaa cgctgtcgct
    28141 gtcgataaag cgcaccagga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
    28201 gccgctaatg ttcaggctgc aactgtcatt aaataaggtg ccagttccaa tcaacgccag
    28261 cgagctttta gcatcggcct ttgcgttctg gcgatggttg ataaaggtga tttcgctttg
    28321 ccactggctg cactgttctt taagttccca cgccggacga gcatgtagac cgttaggcaa
    28381 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
    28441 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagttg
    28501 ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc gcgcaatgtc cgtggcgggt
    28561 ggcaggcccc attaccactg tgggtacgcc agtatcggtt aagtgcaccg ctccaccgtc
    28621 cgtgccgccg ttactgaaca tatccgcctg caacggaacg ccaatttctg ccgacacggt
    28681 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
    28741 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    28801 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtagcag tttgcccgcc
    28861 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gcagttcggc
    28921 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcggt catcaaaggc
    28981 tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc
    29041 gcctggacga attcccgcct gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg
    29101 catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    29161 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca cgttgccaac
    29221 cggcagcaca tcaattgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    29281 catatgcgca cagatcatca ccttcggacc tgtcgattca ttgaggcgga tcagcaccga
    29341 tcccagacca tcaaatcgta cttctttttg caggcgatcc gcttcttcca gtaggatctg
    29401 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
    29461 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    29521 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctggcaaa
    29581 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt atcccgcggt
    29641 gaaaagcgtg gatcttcgtg tacttcaata ccgatagcat gaccggtgtt gtgaccaaaa
    29701 tagtcgccaa atcctgcctc ggtaatcacc cggcgcgcgg cgtcgtcaac ctgttagcag
    29761 cgtacgccag ggcgaatcgc ggagattgcc gccagctgtg cttgcaggac aatctgatag
    29821 acgttaaaca gcgggtgaga ttcggcaatt accccttcgc cattcaccag caaggtgcgc
    29881 gtcatatcag agcagtaccc ctgatagagc gcaccaaaat caagagtgac aaactcgcct
    29941 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccacgcca gccgctggcg
    30001 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    30061 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    30121 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    30181 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    30241 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    30301 atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag
    30361 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    30421 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    30481 tgccgtgagg agagaagcac tgcatccagt tgttgcgcct taagccagtc gcgcagcgaa
    30541 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttacctttac
    30601 ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc cccagcgcga
    30661 tccccgccat atagacgccc aggttagtga ccagcggcca tgcccaaata gcggattccg
    30721 ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaatc gcgccgacca
    30781 tatacgacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    30841 tccccataaa ggcaaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    30901 ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag acccagacct atcggcggaa
    30961 taacgatagc gatagaacgt gcagtgaccg gtaatacgtg gtcagtggta aaactgaagg
    31021 caacaaaacc tgcggctttg ttgatcgggc caccaaggtc gattgcggtc gccgcggcga
    31081 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcacg gtacggatac
    31141 cgccgttgat ccagccgcca aagggggtaa tgacgtagta catcgccagc atgacaaaaa
    31201 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    31261 ggattttctg gttcatccac ttcacaaggt aacccgcgac gatagaaata atcagcgcac
    31321 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    31381 tcagcagttg cgtcggctgg gtagacatca agccgccgat aaaaccagcc gggaacgcca
    31441 gtttgccacc gatagagttc gccacaaaag cagcaaacat cggaatggca aaaccaaaca
    31501 acacgccgcc gaaggactgc gacagccagg caaatttcag taaggagagg tcgaaaccgg
    31561 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    31621 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    31681 tgcgtgaaat ccctaacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    31741 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    31801 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    31861 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    31921 cataaacgtc gcgtgattcg aaacgctcat tatcttccgg ggtaactgcc acggagtgga
    31981 tgatgatggt tgcttcggcg atatcctgcg ccgtcaggcg attctggata ccgtccgcgc
    32041 cctgggtttc aattttcact tcataacctg cttctaccgc cgcttcttcc agcgcctgag
    32101 cggccataaa ggtgtgagcc aggcccattg ggcaggcaca taaggcaatt agtttcttac
    32161 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    32221 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    32281 tgacgcaggt cggccttgtg agggggacac aaagaaaaat tgaattaact gtatgattta
    32341 aaagattatc gggagagtta cctcccgata taacagaaag gatttacaga atgtgcccta
    32401 aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat
    32461 acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga
    32521 aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgccagca atataaaccc
    32581 caccaaaggt tcccagcgtt aatgccagat tgccgccaaa acggcccata atgacgcaaa
    32641 acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat
    32701 cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat
    32761 tcaccagccc aggaccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca
    32821 atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt
    32881 cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg
    32941 ttccggcacc gtaaaccgca ataggtttac cttcgaccgg ttctgcgcca ccaaactgaa
    33001 tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa
    33061 tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat
    33121 ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt
    33181 tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat
    33241 caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa
    33301 gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc
    33361 tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac
    33421 gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact ttatcgtttc
    33481 agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat
    33541 aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc
    33601 gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta
    33661 ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct
    33721 atctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc
    33781 agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accagttccg
    33841 ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc
    33901 ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct
    33961 cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc
    34021 ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc
    34081 agtagtgaag ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt
    34141 gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga
    34201 cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca
    34261 gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat
    34321 ccggtgctcg tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga
    34381 ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc
    34441 agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc
    34501 gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg
    34561 ttgatgctgc atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt
    34621 ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc
    34681 gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg
    34741 ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat
    34801 aatgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct
    34861 ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata
    34921 acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca
    34981 aatgcaggtg aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat
    35041 tacggtaatt actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat
    35101 gaatggcgca aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag
    35161 cgtaaagcat atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga
    35221 aaaggccgcg gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga
    35281 tgcgacgcta gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata
    35341 aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa ctacaatccc
    35401 agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag
    35461 cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg
    35521 aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata
    35581 accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg
    35641 actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc
    35701 cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag
    35761 accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg
    35821 gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga
    35881 ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt
    35941 tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc
    36001 ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa
    36061 ctcgggatca ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg
    36121 acaatgtaag ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagtggtttt
    36181 tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc
    36241 tgcaacaacg agacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc
    36301 aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc
    36361 ggatagtgat cacgaatctg ctccgccaga ttttttccgg tggcaatccc tagtttggca
    36421 gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga
    36481 tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca
    36541 atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg
    36601 ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg
    36661 tttcatgcta tgataaacga gaatgattat caaattcatt taaatggatt gtggtgattt
    36721 ctctgataga ccaggattat gactacgaaa agattgatga cttgaatgtt atggttatgt
    36781 ttaatgttag cacatttaca taactttcag cttccataca caacatagca gaaatgtatg
    36841 acagatcact atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt
    36901 tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc
    36961 aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact
    37021 gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat
    37081 cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg
    37141 cttctccgaa atgttcgaaa aactgctcag atttgccaac gaagggacta acttcgtctt
    37201 tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt
    37261 ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg
    37321 cgcaattggt ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc
    37381 cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag atatcctcgg
    37441 caaaatctcc cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat
    37501 gtccatcgtt ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt
    37561 actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc
    37621 cagtgaagaa aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct
    37681 gggtgaatac attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg
    37741 ctttatcgcc ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg
    37801 ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat
    37861 gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc
    37921 caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc
    37981 tgaaggcatc atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat
    38041 cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct
    38101 gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt
    38161 gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa
    38221 tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta
    38281 tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc
    38341 gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata
    38401 tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg
    38461 tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc
    38521 tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca
    38581 agaatgaccg cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgcccggca
    38641 atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg
    38701 tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc
    38761 gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg
    38821 tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc
    38881 tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc
    38941 acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga
    39001 tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt
    39061 tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg
    39121 ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt
    39181 tccggcccgc tcagcactaa cagcagttca ctacccggca attgatataa cttttcgttt
    39241 tcctgcatca acggcagcag cgtacggtaa attgatcgga tacagtgaac gcgcatcatt
    39301 aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaactcttgc
    39361 ccgcctcttg ctccggcgct tgttccaacg cacggaagtt gggcaggagc gttaacggat
    39421 cggtcagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    39481 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc aagaataaat gccagcgaat
    39541 attcggtttc caccccttgc aaaaagttct ggttgtaatt cagaaggcaa agcgttgaaa
    39601 cagcccaggt gagatttaaa aacggatagc gaagcttacc gaccccgagg gtaaagatga
    39661 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    39721 gaagtagaag cacgcttaac gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc
    39781 ccaacgacgg cgcgatatcc ctgcgccaca atatctgcgc aaagtgggga cttacaatca
    39841 tgcgagtgag atagtagaaa agcatgttgt aaataagcac agcggtgaaa aggcttagca
    39901 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    39961 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    40021 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    40081 ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg ctaccgagca
    40141 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    40201 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    40261 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    40321 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    40381 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    40441 acacaaataa taagtttatt cgcatcgcga agcaaacagg gggccagcct gttgccgatt
    40501 tttcatccaa tgatgctttc accaggcggc actaacccgg acgtagactg tattacaaaa
    40561 gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa atttggtgga
    40621 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    40681 tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa
    40741 gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag
    40801 ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt
    40861 gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt
    40921 ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta
    40981 taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc aaggagagga
    41041 tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt aataaatggg
    41101 tacgcaagga gggctggaca acgtcaccaa aacctggcgt ccagggtggc aaagcccgac
    41161 tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc
    41221 agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg gtgacactgg
    41281 cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga
    41341 ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc
    41401 aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac
    41461 cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt
    41521 gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc
    41581 cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc tcctggagca
    41641 cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga cattgagcag
    41701 ggaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat
    41761 attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt ttatcttttt
    41821 acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt tgttgatacg
    41881 ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg gagactgccc
    41941 cgccccggtt acggcgacac gcagcggcat accaacttta cccataccca cttccagctc
    42001 atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcggc
    42061 cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat gttttttcgc
    42121 cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc
    42181 tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct gcgggccgtt
    42241 acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg
    42301 cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact
    42361 ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac gagtgaagat
    42421 ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg cttctggcaa
    42481 ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg acagtttttt
    42541 accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc
    42601 cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg
    42661 ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc
    42721 ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg gaccacggat
    42781 ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa cacacggttc
    42841 atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc
    42901 tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata
    42961 agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt
    43021 ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat ccataatggc
    43081 ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc
    43141 accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg
    43201 cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt
    43261 ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac
    43321 tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg ttttcgttga
    43381 caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga cgaacgaaca
    43441 atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcgg ctccacacag
    43501 cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43561 atcccgtcat cacccaccaa ctactttatg tagtctccgc cgtgtagcaa gaaattgaga
    43621 agtgggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43681 atcccgtcat cacccaccaa tttctcgcca gctaaatttc ttgtaaaaat gtgaagtacc
    43741 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    43801 cgatcccgtc atcacccacc acttcgggtc gttagctcag ttggtagagc agttgacttt
    43861 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa
    43921 aggcgctttt ttgctatctg cgatactcaa agattcgaac ctgcagcagg tttgagttga
    43981 gcgcagcgaa acaacggagc cgctcgcggc gacggcccga agggcgagcg aagcgagtca
    44041 tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc tatctgcgat
    44101 ttgtaagatt gatttttctt ctcaagttcc ttgttcttct gccgatatta cctcgtacag
    44161 tgacgaggac tgaacaatgg ccactatctc ttcaacttca attccttcca tacaaacaca
    44221 gagtagtaac agggcatcac aaggtagcga tgttgcttca aaaatcgcgc ggataagtca
    44281 gcaaatcatc aaactcactc aacaaattaa agagattgta gacactagcg gcagtgctga
    44341 agacaaacaa aaacaggccg aattgattca acagcaaatc accctactgg aaacacaact
    44401 ggcgcagttg cagagacagc aggcagaaaa ggcgcaggag aaagagcagc gtttgtctct
    44461 gaatgtatcc cttctaaatc ctgttgaaaa tacgactcat atcgatatct atatctgata
    44521 caacggagcc ccttcccaac cagccattag cgcattgccc tggtctcgta ccacgtccca
    44581 taacgcttca gcggcggtgg aaagtgaacg gttcttcctg cgcaccagca tcaactggcg
    44641 ttcaacaacc ggcgtaatgc gctttaccac cagtgggcta ccttcaggca atggtagcgc
    44701 cagtgcggga agaatactga tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg
    44761 atgtccaatc tcctgcacaa tattcgcctg aataccattg cgcgccagcg ctgcgtcaat
    44821 cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat ttcgctccct gtaatgcctg
    44881 ccagggtaca taatcttcca cggccaaagc gctatcgcga tggcaaagca gaaagaaagg
    44941 ctcggaaaga atcgcttcac attgcagatc gcccactggg cctggatcga tgacgatgca
    45001 aaaatcgaca tctccctggc gaatactttc catcacccac tgctgcggac gatcgtgcag
    45061 gacaaactga atatctggat agcggcgatg gctttcagca atgcattgcg gaataagatg
    45121 cgcggaaatg gtctggctgg cagcgacccg aacttttccg ctcagttgtt gccccatacg
    45181 cccggtatcg cgcaacgtgc tgttaagttc atccagcagt cgctcaagac gcaaagccaa
    45241 ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc
    45301 agtatgattt tccagctcct tcacactgtg acttactgcc gactggctca agccgatacg
    45361 ctctcctgca cgactaaaac ttttctcctg cgctactgtg acgaaaacct ttaattgttt
    45421 taaagaataa ttcatctgtt ttcttcatga atggatgcaa taaatcaatt ttatttctca
    45481 aactggaaga agcacaatag aaccatcgat catctggagt ctttatgaaa ctttttcgta
    45541 tcctcgatcc tttcacctta accctgatca cggtggtgtt gctggcctct ttctttccgg
    45601 ccagaggcga tttcgtcccc ttctttgaaa atctgaccac cgcagctatt gccctgctgt
    45661 tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc
    45721 gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt
    45781 ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta ctccggtttt ctctacttgt
    45841 gcattctccc ggctaccgtg cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg
    45901 tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct ggggattttc ctttcaccat
    45961 tgctagttgg tctggtgatg aatgttcacg gtgcaggggg cagccttgag caggtcggta
    46021 aaattatgct gcaactgctg ctgccgtttg tgttggggca tctttcccgg ccgtggattg
    46081 gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt
    46141 tggtggttta tacagcgttc agcgaagccg tcgttaatgg tatctggcat aaagttggct
    46201 ggggatcatt gctgtttatc gtggtggtca gctgcgttct tctggctatc gtgattgtag
    46261 ttaacgtctt tatggcacgc cgactgggct tcaataaggc agatgaaatt actatcgtct
    46321 tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca
    46381 catcggtgat cggtatgatg gtgctgcccc tgatgatttt ccatcagatc caattgatgg
    46441 tctgtgcggt gctggcgcgt cgatacaaac gccagaccga acagttacag gcgcagcagg
    46501 aaagcagcgc cgataaagct taaagcggac gcttcagggg ctggaccagt tgcgtcagcc
    46561 cctcggtttt aatcagcagc gtgatttgca ttagctcacc gagcttccct gccgggaact
    46621 catctttgcg ggcaaaccac agcagatact cctccggcag gtcgattaag cgacgccctt
    46681 tgtatttgcc aaacggcatt atcgtattgg ctatttcaat cagctgctct ttttccatct
    46741 cagctaccca gcaaacgcag catttccgct tcgtcgatga cttcaatgcc cagttcctgc
    46801 gccttcgcca gtttagatcc tgcagcttca cccgctatca ccagatcggt tttcttcgac
    46861 acgctgcccg cgactttcgc gcccagttcg accagtcgag ctttagcgtc atcacgcgac
    46921 atctggctta agctgcccgt aagcaccacg gttttaccag caaacgggct gtcaatctct
    46981 tccgcgttga taacgatcgg cgcaggccag tgaacacctt ccgccaacag ctcgctgatg
    47041 acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca
    47101 acatcaggca ccttttgcag ctcttcaatc gaagcggctt ccagcacttc cagcgtgccg
    47161 aaatatgccg ccagacctgc tgcggtggcc tcgccgactt cacggatgcc aagtgcatag
    47221 aggaagcgag caaaggtggt ttctttcgct ttttccagcg cgttaaccac gttttgtgcc
    47281 gattttggcc ccatacgctc cagtccggtc agtttgcctg cggtgagttt gaacagatct
    47341 gccggagtgt ggacatattc tttttcaacc agctgatcga tgattttgtc gcccattccg
    47401 tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg
    47461 caaatcaggc caccggtaca gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa
    47521 ccacataccg gacaatgcgt cgggaataca acctcacggg tatcttccgg gcgttcagaa
    47581 agcacgacgt taaccacctg cgggatcacg tcgccagcgc ggcgaatcac cactttatcg
    47641 ccaatgcgta aaccaagacg ttcgatttca tccgcattgt gtaaggttgc gttactcacc
    47701 agcacgccag caacatggac aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc
    47761 ccaacctgaa actcgacgtc acgcacaaag gtcatctgct cctgcgccgg gaatttaaac
    47821 gctaccgccc agcgcggggc acgcgcgaca aagccaagct gctcctgctg ttccagtgag
    47881 ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg tcgggcggtc ttcttccact
    47941 ttgtgataga acgccagcac ttcttccgcc gattcacaaa gcgttacccg atcgctgacc
    48001 ggcaaccccc actttttaaa ttgcagtaaa cggccaagat gagtatccgg cagctcgcca
    48061 ccttccagaa caccaacgcc atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc
    48121 ggatcaagct gacgcagtga accagctgcc gcattacgtg ggttagcaaa aactttcccg
    48181 cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg cctgcggcag gaacacttca
    48241 ccacgcactt ccagacgcgc cgggatattc tctccgtgca gcttcagcgg aatggcgcga
    48301 atagtacgca cattagacgt gatatcttcc ccggtggtgc catcgccacg ggtcgcggca
    48361 ctgactaaaa cgccattttc atacagaata ctgacggcaa ggccatccag cttcagctca
    48421 cagcaccagg tgactttctc gttgtttttc agacggtcct gcacacgttt gttgaaagca
    48481 agaaagcttt cttcatcaaa aacgttatcc agtgacagca ttggtacttc atggcgtatc
    48541 tggctgaaag ccgccagcgg cgcagcacct acacgttggg taggcgaatc aggcgtaatc
    48601 agttctggat gtttggtttc cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg
    48661 tcgggaattt ccggcgcatc catcacatga taaagatatt catgatggcg aagcgtcgtt
    48721 cgcagttctg tcagttgttg ttcgattgat tccatatcgc accatcaatg ctaaaaaccc
    48781 ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc
    48841 tttgacttcg cggatgatgt cctggtactc gcgcaatttc tgcggagtca tcatacggcg
    48901 ctgatcgtca agcacgacac cgcccacttc atcggcaata tgctgcgcag attgcagcat
    48961 cagcttgaag ttctgcagct cgtcaccgta agacggtacc tgcataaaga tagtgacacc
    49021 cggagtagtg aaatccttca tttcaggatc aaaggttccc ggtttcacca tattcgccag
    49081 gctgaataac gccgggccgc tgccatccgg gctaagatga cgatggtaaa tattcatatc
    49141 gccaaaaatg aagcccgctt gttgaatgct gttaagaagc agttcaccgt ttagctcgct
    49201 accgtgatgc gccgcgacgt tcatgataat caccgcttct ttgcgcttcg gtttatccat
    49261 aactggagcc ggttccgcta caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg
    49321 gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg
    49381 tggcgaaact ggctgctgca acggctgttc aggctgcggc tgataggcag gctgctgcat
    49441 cggctgcgct ggacgcggag catgttgcgg cggtacctgc gcttcaggcg gctgctggat
    49501 cggttgacgc ggctgcgcag acgcataagg cggttggtac tggtgttgcg gcgacggacg
    49561 agcagcctca tgctcctgag cgttagccgg ggcatgattc acgcggtgaa cacgaacctc
    49621 accaacgccc tcatcatctt cgacatcctc gtcataagaa tcgtcgtcac gttttgactt
    49681 cattcgtttt aatggccgat cgcggaacat agaagatcgt tctttacggc tggtccagaa
    49741 accatgtacc agtaaagcga ttatggcgat cgcgccaaca atgattaata tcagacgcaa
    49801 atcctgcatc attatattct ctgttgttct aacaccttgc caccacggca aacatttact
    49861 cactaagagt atttgccgat tacctcaagt gcaagtgcac tattaacttt cacagcacaa
    49921 agatagatga aatcgtgctt tttgctgttt tttcgaacat atcctaactg tccattgcgc
    49981 aattacccgg taaaatacgc agaattttcc tgggattggt caaaaggagc tcatcctgat
    50041 tatggtttca tcattcacat ctgccccacg cagcggtttt tactattttg cgcaaggctg
    50101 gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt ttaccgctgc tggtcaatat
    50161 tttgttgatg gggggcgcat tctggtggct ctttacacag ctcgatgtct ggatcccgac
    50221 tctcatgagt tacgttccgg actggctaca atggctgagt tatctgttgt ggcctctggc
    50281 tgtcatctct gtgctgttag tgtttggcta tttcttctcc acgattgcta actggattgc
    50341 cgctccgttt aacggtttat tggctgaaca actggaagca cgattgactg gcgctacacc
    50401 gccagatacc gggattttcg gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg
    50461 gcaaaaattt gcctggtatc tgccgcgcgc aattgtatta ctaattcttt acttcatccc
    50521 tggtattggg caaaccgtcg cgccggtact gtggttcctg tttagcgcct ggatgttagc
    50581 catccagtat tgcgattacc ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac
    50641 cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt gctttaacca gcctgtttac
    50701 gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat
    50761 gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg taacaatcta ccggttattt
    50821 tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc ccttattcca tcttgcatgt
    50881 cattatttcc cttctgtata tagatatgct aaatccttac ttccgcatat tctctgagcg
    50941 ggtatgctac ctgttgtatc ccaatttcat acagttaagg acaggccatg agtaagattt
    51001 ttgaagataa ctcgctgact atcggtcaca cgccgctggt tcgcctgaat cgcatcggta
    51061 acggacgcat tctggcgaag gtggaatctc gtaaccccag cttcagcgtt aagtgccgta
    51121 tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac
    51181 tggttgaacc gaccagcggt aataccggga ttgcactggc ctatgttgct gccgctcgcg
    51241 gttacaaact caccctgacc atgccagaaa ccatgagtat tgaacgccgc aagctgctga
    51301 aagcgttagg tgcaaaccta gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc
    51361 aaaaagcaga agaaattgtc gccagcaatc cagagaaata cctgctgctg caacaattca
    51421 gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg tccggagatc tgggaagata
    51481 ccgacggtca ggttgatgta tttattgctg gcgttgggac tggcggtacg ctgactggcg
    51541 tcagccgcta cattaaaggc accaaaggca agaccgatct tatctctgtc gccgttgagc
    51601 caaccgattc tccagttatc gcccaggcgc tggcaggtga agagattaaa cctggcccgc
    51661 ataaaattca gggtattggc gctggtttta tcccggctaa cctcgatctc aagctggtcg
    51721 ataaagtcat tggcatcacc aatgaagaag ccatttctac cgcccgtcgt ctaatggaag
    51781 aagaaggtat tcttgcaggt atctcttctg gagcagctgt tgccgcggcg ttgaaactac
    51841 aagaagatga aagctttacc aataagaata ttgtggttat tctaccatca tcgggtgagc
    51901 gttatttaag caccgcattg tttgccgatc tcttcactga gaaagaattg caacagtaat
    51961 gccagcttgt taaaaatgcg taaaaaagca cctttttagg tgcttttttg tggcctgctt
    52021 caaactttcg cccctcctgg cattgattca gcctgtcgga actggtattt aaccagacta
    52081 attattttga tgcgcgaaat taatcgttac aggaaaagcc aaagctgaat cgattttatg
    52141 atttggttca attcttcctt taacggcata atgtttaatg acgtacgaaa cgtcagcggt
    52201 caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg
    52261 gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga ctttagttcc acaacactaa
    52321 acctataagt tggggaaata caatgttcca gcaagaagtt accattaccg ctccgaacgg
    52381 tctgcacacc cgccctgctg cccagtttgt aaaagaagct aagggcttca cttctgaaat
    52441 tactgtgact tccaacggca aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct
    52501 gggcctgact caaggtaccg ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc
    52561 ggttgaacat ctggttaaac tgatggcgga actcgagtaa tttcccgggt tcttttaaaa
    52621 atcagtcaca agtaaggtag ggttatgatt tcaggcattt tagcatcccc gggtatcgct
    52681 ttcggtaaag ctctgcttct gaaagaagac gaaattgtca ttgaccggaa aaaaatttct
    52741 gccgaccagg ttgatcagga agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc
    52801 cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc
    52861 tttgaagggc atattatgct gctcgaagat gaggagctgg agcaggaaat catagccctg
    52921 attaaagata aacacatgac agctgacgca gctgctcatg aagttatcga aggtcaggct
    52981 tctgccctgg aagagctgga tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat
    53041 atcggtaagc gcctgctgcg caacatcctg ggcctgaaga ttatcgacct gagcgccatt
    53101 caggatgaag tcattctggt tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac
    53161 ctgaagaagg tgctgggttt catcaccgac gcgggtggcc gtacttccca cacctctatc
    53221 atggcgcgtt ctctggaact gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg
    53281 aaaaatgacg actatctgat tctggatgcc gtaaataatc aggtttacgt caatccaacc
    53341 aacgaagtta ttgataaaat gcgcgctgtt caggagcaag tggcttctga aaaagcagag
    53401 cttgctaaac tgaaagatct gccagctatt acgctggacg gtcaccaggt agaagtatgc
    53461 gctaacattg gtacggttcg tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt
    53521 ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa
    53581 cagtttgctg cttacaaagc agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt
    53641 accatggaca tcggcggcga caaagagctg ccatacatga acttcccgaa agaagagaac
    53701 ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc gtaaagagat cctgcgcgat
    53761 cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat tgcgcattat gttcccgatg
    53821 atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa
    53881 ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca
    53941 ccggctgccg caacaattgc acgtcattta gccaaagaag ttgatttctt tagtatcggc
    54001 accaatgatt taacgcagta cactctggca gttgaccgtg gtaatgatat gatttcacac
    54061 ctttaccagc caatgtcacc gtccgtgctg aacttgatca agcaagttat tgatgcttct
    54121 catgctgaag gcaaatggac tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca
    54181 cttctgttgc tggggatggg tctggacgaa ttctctatga gcgccatttc tatcccgcgc
    54241 attaagaaga ttatccgtaa cacgaacttc gaagatgcga aggtgttagc agagcaggct
    54301 cttgctcaac cgacaacgga cgagttaatg acgctggtta acaagttcat tgaagaaaaa
    54361 acaatctgct aatccacgag atgcggccca atttactgct taggagaaga tcatgggttt
    54421 gttcgataaa ctgaaatctc tggtttccga cgacaagaag gataccggaa ctattgagat
    54481 cattgctccg ctctctggcg agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc
    54541 ggaaaaaatc gttggtgatg gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc
    54601 tgtagacggt accattggta aaatctttga aaccaatcac gcgttctcta tcgaatctga
    54661 tagcggcgtt gaactgttcg tccacttcgg tatcgacacc gttgaactga aaggcgaagg
    54721 cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt
    54781 tgatctgccg ctgctggaag agaaagccaa gtctaccctg actccggttg ttatctccaa
    54841 catggacgaa atcaaagaac tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc
    54901 ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa atggcgccca tcg
//
LOCUS       DAZPBM010000007        52786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPBM010000007 DAZPBM010000000
VERSION     DAZPBM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.007"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(22..648)
                     /gene="upp"
                     /locus_tag="ACHUZQ_000354"
     CDS             complement(22..648)
                     /gene="upp"
                     /locus_tag="ACHUZQ_000354"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1024151.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            973..2010
                     /gene="purM"
                     /locus_tag="ACHUZQ_000355"
     CDS             973..2010
                     /gene="purM"
                     /locus_tag="ACHUZQ_000355"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1024152.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2010..2648
                     /gene="purN"
                     /locus_tag="ACHUZQ_000356"
     CDS             2010..2648
                     /gene="purN"
                     /locus_tag="ACHUZQ_000356"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1024153.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2819..4885
                     /gene="ppk1"
                     /locus_tag="ACHUZQ_000357"
     CDS             2819..4885
                     /gene="ppk1"
                     /locus_tag="ACHUZQ_000357"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1024154.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4890..6431
                     /gene="ppx"
                     /locus_tag="ACHUZQ_000358"
     CDS             4890..6431
                     /gene="ppx"
                     /locus_tag="ACHUZQ_000358"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1024155.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6470..8713)
                     /gene="pdeF"
                     /locus_tag="ACHUZQ_000359"
     CDS             complement(6470..8713)
                     /gene="pdeF"
                     /locus_tag="ACHUZQ_000359"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1024156.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(8895..9047)
                     /locus_tag="ACHUZQ_000360"
     CDS             complement(8895..9047)
                     /locus_tag="ACHUZQ_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024157.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            9065..9256
                     /locus_tag="ACHUZQ_000361"
     CDS             9065..9256
                     /locus_tag="ACHUZQ_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1024158.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            9567..10085
                     /locus_tag="ACHUZQ_000362"
     CDS             9567..10085
                     /locus_tag="ACHUZQ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1024159.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            10101..10640
                     /locus_tag="ACHUZQ_000363"
     CDS             10101..10640
                     /locus_tag="ACHUZQ_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1024160.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(10735..12312)
                     /gene="guaA"
                     /locus_tag="ACHUZQ_000364"
     CDS             complement(10735..12312)
                     /gene="guaA"
                     /locus_tag="ACHUZQ_000364"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1024161.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(12381..13847)
                     /gene="guaB"
                     /locus_tag="ACHUZQ_000365"
     CDS             complement(12381..13847)
                     /gene="guaB"
                     /locus_tag="ACHUZQ_000365"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1024162.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14009..15379
                     /gene="xseA"
                     /locus_tag="ACHUZQ_000366"
     CDS             14009..15379
                     /gene="xseA"
                     /locus_tag="ACHUZQ_000366"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1024163.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(15376..15591)
                     /locus_tag="ACHUZQ_000367"
     CDS             complement(15376..15591)
                     /locus_tag="ACHUZQ_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1024164.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(15660..17132)
                     /gene="der"
                     /locus_tag="ACHUZQ_000368"
     CDS             complement(15660..17132)
                     /gene="der"
                     /locus_tag="ACHUZQ_000368"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1024165.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(17250..18428)
                     /gene="bamB"
                     /locus_tag="ACHUZQ_000369"
     CDS             complement(17250..18428)
                     /gene="bamB"
                     /locus_tag="ACHUZQ_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1024166.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(18439..19059)
                     /gene="yfgM"
                     /locus_tag="ACHUZQ_000370"
     CDS             complement(18439..19059)
                     /gene="yfgM"
                     /locus_tag="ACHUZQ_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1024167.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(19077..20351)
                     /gene="hisS"
                     /locus_tag="ACHUZQ_000371"
     CDS             complement(19077..20351)
                     /gene="hisS"
                     /locus_tag="ACHUZQ_000371"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1024168.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(20462..21580)
                     /gene="ispG"
                     /locus_tag="ACHUZQ_000372"
                     /gene_synonym="gcpE"
     CDS             complement(20462..21580)
                     /gene="ispG"
                     /locus_tag="ACHUZQ_000372"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1024169.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(21607..22620)
                     /gene="rodZ"
                     /locus_tag="ACHUZQ_000373"
     CDS             complement(21607..22620)
                     /gene="rodZ"
                     /locus_tag="ACHUZQ_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1024170.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(22905..24059)
                     /locus_tag="ACHUZQ_000374"
     CDS             complement(22905..24059)
                     /locus_tag="ACHUZQ_000374"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1024171.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(24209..24640)
                     /gene="ndk"
                     /locus_tag="ACHUZQ_000375"
     CDS             complement(24209..24640)
                     /gene="ndk"
                     /locus_tag="ACHUZQ_000375"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1024172.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(24789..27101)
                     /gene="pbpC"
                     /locus_tag="ACHUZQ_000376"
     CDS             complement(24789..27101)
                     /gene="pbpC"
                     /locus_tag="ACHUZQ_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1024173.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(27102..32063)
                     /gene="yfhM"
                     /locus_tag="ACHUZQ_000377"
     CDS             complement(27102..32063)
                     /gene="yfhM"
                     /locus_tag="ACHUZQ_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR1024174.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            32270..33115
                     /gene="sseA"
                     /locus_tag="ACHUZQ_000378"
     CDS             32270..33115
                     /gene="sseA"
                     /locus_tag="ACHUZQ_000378"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR1024175.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            33451..33576
                     /gene="timP"
                     /locus_tag="ACHUZQ_000379"
     CDS             33451..33576
                     /gene="timP"
                     /locus_tag="ACHUZQ_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1024176.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(33608..34384)
                     /gene="sseB"
                     /locus_tag="ACHUZQ_000380"
     CDS             complement(33608..34384)
                     /gene="sseB"
                     /locus_tag="ACHUZQ_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1024177.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(34526..35809)
                     /gene="pepB"
                     /locus_tag="ACHUZQ_000381"
     CDS             complement(34526..35809)
                     /gene="pepB"
                     /locus_tag="ACHUZQ_000381"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1024178.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(35987..36187)
                     /gene="iscX"
                     /locus_tag="ACHUZQ_000382"
     CDS             complement(35987..36187)
                     /gene="iscX"
                     /locus_tag="ACHUZQ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1024179.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(36199..36534)
                     /gene="fdx"
                     /locus_tag="ACHUZQ_000383"
     CDS             complement(36199..36534)
                     /gene="fdx"
                     /locus_tag="ACHUZQ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1024180.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(36536..38386)
                     /gene="hscA"
                     /locus_tag="ACHUZQ_000384"
     CDS             complement(36536..38386)
                     /gene="hscA"
                     /locus_tag="ACHUZQ_000384"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1024181.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(38403..38918)
                     /gene="hscB"
                     /locus_tag="ACHUZQ_000385"
     CDS             complement(38403..38918)
                     /gene="hscB"
                     /locus_tag="ACHUZQ_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1024182.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(39014..39337)
                     /gene="iscA"
                     /locus_tag="ACHUZQ_000386"
     CDS             complement(39014..39337)
                     /gene="iscA"
                     /locus_tag="ACHUZQ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1024183.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(39354..39740)
                     /gene="iscU"
                     /locus_tag="ACHUZQ_000387"
     CDS             complement(39354..39740)
                     /gene="iscU"
                     /locus_tag="ACHUZQ_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1024184.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(39768..40982)
                     /gene="iscS"
                     /locus_tag="ACHUZQ_000388"
     CDS             complement(39768..40982)
                     /gene="iscS"
                     /locus_tag="ACHUZQ_000388"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1024185.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(41223..41711)
                     /gene="iscR"
                     /locus_tag="ACHUZQ_000389"
     CDS             complement(41223..41711)
                     /gene="iscR"
                     /locus_tag="ACHUZQ_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1024186.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(42012..42749)
                     /gene="trmJ"
                     /locus_tag="ACHUZQ_000390"
     CDS             complement(42012..42749)
                     /gene="trmJ"
                     /locus_tag="ACHUZQ_000390"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1024187.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            42868..43671
                     /gene="suhB"
                     /locus_tag="ACHUZQ_000391"
     CDS             42868..43671
                     /gene="suhB"
                     /locus_tag="ACHUZQ_000391"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1024188.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            43816..44670
                     /locus_tag="ACHUZQ_000392"
     CDS             43816..44670
                     /locus_tag="ACHUZQ_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1024189.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            44861..46141
                     /gene="csiE"
                     /locus_tag="ACHUZQ_000393"
     CDS             44861..46141
                     /gene="csiE"
                     /locus_tag="ACHUZQ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1024190.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(46133..47272)
                     /locus_tag="ACHUZQ_000394"
     CDS             complement(46133..47272)
                     /locus_tag="ACHUZQ_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1024191.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(47432..48322)
                     /gene="hcaR"
                     /locus_tag="ACHUZQ_000395"
     CDS             complement(47432..48322)
                     /gene="hcaR"
                     /locus_tag="ACHUZQ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1024192.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            48458..49819
                     /gene="hcaE"
                     /locus_tag="ACHUZQ_000396"
     CDS             48458..49819
                     /gene="hcaE"
                     /locus_tag="ACHUZQ_000396"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1024193.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            49816..50334
                     /gene="hcaF"
                     /locus_tag="ACHUZQ_000397"
     CDS             49816..50334
                     /gene="hcaF"
                     /locus_tag="ACHUZQ_000397"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1024194.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            50334..50654
                     /locus_tag="ACHUZQ_000398"
     CDS             50334..50654
                     /locus_tag="ACHUZQ_000398"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1024195.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            50651..51463
                     /gene="hcaB"
                     /locus_tag="ACHUZQ_000399"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             50651..51463
                     /gene="hcaB"
                     /locus_tag="ACHUZQ_000399"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1024196.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            51473..52675
                     /gene="hcaD"
                     /locus_tag="ACHUZQ_000400"
     CDS             51473..52675
                     /gene="hcaD"
                     /locus_tag="ACHUZQ_000400"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1024197.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        12743 a        14267 c        13157 g        12619 t
ORIGIN      
        1 ggctttaatt atttttattc tttatttcgt accaaagatt ttgtcaccgg catcgccgag
       61 gcccggaata atgtatccgt gctcgttcag tccctggtca atcgatgcgg tatacagttc
      121 gacgtccggg tgcgcttttt ccagcgcagc gataccttct ggcgcagcta ccagcaccag
      181 aactttgatg ctgctgcagc ccgctttttt cagcaggtcg atggtcgcga taacggaacc
      241 accggttgcc agcattgggt caacgatcag cgccatacgc tcatcgatgt tagaaaccag
      301 tttctggaag tacggtaccg gctccagcgt ttcttcatta cggtacatac cgacaacgct
      361 gatgcgcgcg ctcggaacat tttccagcac accgtccatc atccccagac cggcacgcag
      421 aattggcaca acggtaattt tcttaccttt gatctggtcg atttctaccg ggccgttcca
      481 gccttcgata gttacttttt ccgtttcgag gtcggcggtc gcttcgtaag tcagcaggct
      541 acccacttcg gaagcgagtt cgcgaaagcg cttggtgctg atatcttgct cacgcatcag
      601 tcccagcttg tgtttgacga gtgggtgttt gacttccacg atcttcatac tctttctcct
      661 ttgaggggca gccacaaaaa aaatcgacgg attatacctc ctttcttcaa ggcggcaata
      721 ttcttttcgt tgactttagt caaaatgata acggtttgag ataaagttat tttatattca
      781 aatggttatg aaagaagatt attccatccg aaaactaacc tttgccctgg cacaagtctt
      841 ctttcgccgc gcgtctgggg aaaagacgtg caaaaaggtt gtgcaaagca gtctcgaaaa
      901 cgtttgcttt ccctgttaga attgcgccga attttatttt tctaccgcaa gtaacgcgtg
      961 gggacccaag cagtgaccga taaaacctct cttagctaca aagatgccgg tgttgatatt
     1021 gacgcgggta atgctctggt tggaagaatc aaaggcgtag tgaagaaaac gcgtcgtccg
     1081 gaagtgatgg gcggtctggg cggcttcggt gcgctgtgtg cattgccgca aaaatatcgt
     1141 gaacccgtgc tggtttccgg cactgacggc gtaggtacca agctgcgtct ggcaatggac
     1201 ttaaaacgtc acgacaccat tggtattgat ctggtcgcca tgtgcgttaa tgacctggtg
     1261 gtgcaaggtg cagaaccgct gtttttcctc gactattacg caaccggaaa actggatgtt
     1321 gataccgctt cagcggtgat cagcggtatc gcggaaggat gtctgcaatc gggctgttca
     1381 ctggtgggtg gcgaaacggc agaaatgccg gggatgtatc acggtgagga ttacgatgtc
     1441 gcgggtttct gcgttggcgt ggtagaaaaa tcagaaatca tcgacggctc taaagtcagc
     1501 gacggcgatg tgctgattgc actcggttcc agcggtccgc actcgaacgg ctattcgctg
     1561 gtgcgcaaaa ttcttgaagt cagcggttgt gatccgcaaa ccaccgaact tgatggtaag
     1621 ccattagccg atcatctgct ggcaccgacc cgcatttacg tgaagtcagt gctggagttg
     1681 attgaaaagg tcgatgtgca tgccattgcg cacctgaccg gcggcggctt ctgggaaaac
     1741 attccgcgcg tattgccaga taatactcag gcagtgattg atgaatcttc ctggcagtgg
     1801 ccggaagtgt tcaactggct gcaaacggct ggtaacgttg agcgccatga aatgtatcgc
     1861 accttcaact gcggcgtcgg gatgattatc gccctgcctg ctccggaagt ggacaaagcc
     1921 ctcgccctgc taaatgccaa cggtgaaaac gcgtggaaaa tcggtatcat caaagcctct
     1981 gattccgaac aacgcgtggt tatcgaataa tgaatattgt ggtgcttatt tccggcaacg
     2041 gaagtaattt acaggcaatt attgacgcct gtaaaaccaa caaaattaaa ggcaccatac
     2101 gggcagtttt cagcaataag gccgacgcgt tcggccttga acgcgcccgc caggcgggta
     2161 ttgcaacgca tacgctcatc gccagcgcgt ttgacagtcg tgaagcctat gaccgggagt
     2221 tgattcatga aatcgacatg tacgcacccg atgtggtcgt gctggctggt tttatgcgca
     2281 ttctcagccc ggcgtttgtc tcccactatg ccgggcgttt gctgaacatt cacccttctc
     2341 tgctgccgaa atatcccgga ttacacaccc atcgtcaggc gctggaaaat ggtgatgaag
     2401 agcacggtac atcggtgcat ttcgtcaccg atgaactgga cggtggcccg gttattttac
     2461 aggcgaaagt cccggtattt gctggtgata cggaagatga catcaccgcc cgcgtgcaaa
     2521 cccaggaaca cgctatttat ccactggtga ttagctggtt tgccgatggt cgtctgaaaa
     2581 tgcacgaaaa cgccgcgtgg ctggatggtc aacgtctgcc gccgcagggc tacgctgccg
     2641 acgagtaatg ccccgtagtt aaagcgccag ctctgccgct ggcgtttttc aattcacctg
     2701 taaatcgcaa gctccagcag tttttttccc ccttttctgg catagttgga catctgccaa
     2761 tattgctcgc cataatatcc aggcagtgtc ccgtgaataa aacggagtaa aagtggtaat
     2821 gggtcaggaa aagctataca tcgaaaaaga gctcagttgg ttatcgttca atgaacgcgt
     2881 gcttcaggaa gcggcggaca aatctaaccc gctgattgaa aggatgcgtt tcctggggat
     2941 ctattccaat aaccttgatg agttctataa agtccgcttc gctgaactga agcgacgcat
     3001 cattattagc gaagaacaag gctccaactc tcattcccgc catttactgg gcaaaattca
     3061 gtcccgggtg ctgaaagccg atcaggaatt cgacggcctc tacaacgagc tgctgctgga
     3121 gatggcgcgt aaccagatct tcctgattaa tgaacgccag ctctccgtca atcaacaaaa
     3181 ctggctgcgt cattatttta agcagtatct gcgtcagcac attacgccga ttttaatcaa
     3241 tcctgacact gacttagtgc agttcctgaa agatgattac acctatctgg cggtggaaat
     3301 tatccgtggc gataccatcc gttacgcgct gctggagatc ccatcagata aagtgccgcg
     3361 ctttgtgaat ttaccgccag aagcaccgcg tcgacgcaag ccgatgattc ttctggataa
     3421 cattctgcgt tactgccttg atgatatttt caaaggcttc tttgattatg acgcgctgaa
     3481 tgcctattca atgaagatga cccgcgatgc cgaatacgat ttagtgcatg agatggaagc
     3541 cagcctgatg gagttgatgt cttccagtct caagcagcgt ttaactgctg agccggtgcg
     3601 ttttgtttat cagcgcgata tgcccaatgc gctggttgaa gtgttacgcg aaaaactgac
     3661 tatttcccgc tacgactcca tcgtccccgg cggtcgttat cataatttta aagactttat
     3721 taatttcccc aatgtcggca aagccaatct ggtgaacaaa ccactgccgc gtttacgcca
     3781 tatttggttt gataaagccc agttccgcaa tggttttgat gccattcgcg aacgcgatgt
     3841 gttgctctat tatccttatc acacctttga gcatgtgctg gaactgctgc gtcaggcttc
     3901 gttcgacccg agcgtactgg cgattaaaat taacatttac cgtgtggcaa aagattcacg
     3961 tatcatcgac tcgatgatcc acgctgcgca caacggcaag aaagtgaccg tggtggttga
     4021 gttacaggcg cgtttcgacg aagaagccaa cattcactgg gcgaagcgcc tgaccgaagc
     4081 aggcgtgcac gttatcttct ctgcgccggg gctgaaaatt cacgccaaac tgttcctgat
     4141 ttcacgtaaa gaaaacggtg aagtggtccg ttacgcacac atcgggaccg ggaactttaa
     4201 cgaaaaaacc gcgcgtcttt atactgacta ttcgttgctg accgcagatg cgcgtatcac
     4261 caacgaagta cggcgggtat ttaactttat tgaaaaccca taccgcccgg tgacatttga
     4321 ttatttaatg gtgtcaccgc aaaactctcg ccgtctgtta tatgaaatgg tagaccgcga
     4381 aatcgccaac gcgcagcaag ggctgcccag tggtatcacc ctgaagctaa ataaccttgt
     4441 cgataaaggc ctggttgatc gtctgtatgc ggcctccagc tccggcgtac cggttaatct
     4501 gctggttcgc ggaatgtgtt cgctgatccc caatctggaa ggcattagcg acaacattcg
     4561 tgccatcagt attgttgacc gttaccttga acatgaccgg gtttatattt ttgaaaatgg
     4621 cggcgataaa aaggtctacc tttcttccgc cgactggatg acgcgcaata ttgattatcg
     4681 tattgaagtg gcgacaccgc tgctcgatcc gcgcctgaag cagcgggtgc tggacatcat
     4741 cgacatattg ttcagcgata cggtcaaagc tcgttatatc gataaagaac tcagtaatcg
     4801 ctacgttccc cgcggcaatc gccgcaaagt acgggcgcag ttggcgattt atgactacat
     4861 caaatcactc gaacaacctg aataacccta tgccaataca cgataaatcc cctcgtccgc
     4921 aggagtttgc tgcggtcgat cttggttcaa acagttttca catggtcata gcccgtgtgg
     4981 tagatggtgc catgcagatt attggccgcc tgaaacagcg ggtgcatctg gcggacggcc
     5041 tggggccaga taatatgttg agtgaagagg caatgacgcg cggtttgaac tgtctgtcgc
     5101 tgtttgccga acggctacaa gggttttctc ctgccagcgt ctgtatagtc ggcacccata
     5161 cgctgcgtca ggcgctgaac gccactgact ttctgaaacg cgcggaaaag gtcattccct
     5221 acccgattga aattatttcc ggtaatgaag aagcccgtct gatttttatg ggcgtggaac
     5281 atacccaacc ggagaaaggt cgcaaactgg ttattgatat tggcggcgga tctacggaac
     5341 tggtgattgg tgaaaatttc gaaccgattc tcgttgaaag ccgccggatg ggttgtgtca
     5401 gctttgccca gctttacttc cctggcgggg tcatcaataa agagaatttt cagcgcgctc
     5461 gtatggcggc agcacaaaaa ctggaaactt taacctggca attccgtatt cagggctgga
     5521 acgtggcaat gggcgcttcc ggtaccataa aagccgccca tgaagtgtta atggaaatgg
     5581 gcgagaaaga cgggataatt accccggaac gtctggaaaa actggtaaaa gaagttttac
     5641 gtcaccgtaa tttcgcatcg ctgagtttac cgggtctttc cgaagagcgg aaaacagtct
     5701 tcgttcctgg actggcgatt ttatgcggtg tgtttgatgc tttagccatc cgtgaactgc
     5761 gcctttctga cggggcgtta cgcgaaggcg tactgtacga aatggaagga cgtttccgtc
     5821 atcaggatgt gcgtagtcgc accgccagca gcctcgccaa ccagtatcac atcgacagcg
     5881 aacaggcccg acgggtgctg gataccacta tgcaaatgta cgaacagtgg cgggaacagc
     5941 aaccgaagct ggcgcatccg caactggagg cgctactgcg atgggccgcc atgctgcatg
     6001 aggtcgggtt gaatatcaac cacagcggtt tgcatcgcca ctccgcttat attctgcaaa
     6061 acagtgactt gccgggtttt aatcaggaac agcagctgat gatggcgaca ctggtgcgct
     6121 atcaccgtaa agcgattaaa ctcgacgatc tgccgcgctt taccttgttt aagaagaaac
     6181 agttcctgcc actgattcag ctattgcgcc ttggcgtatt actcaacaat caacgtcagg
     6241 caaccaccac accgccaaca ttgacactga ttactgatga cagtcactgg acactgcgtt
     6301 tcccgcatga ctggtttagt cagaatgcgt tggtactgct tgatctggaa aaggagcaag
     6361 aatactggga aggcgtggct ggctggcggt tgaaaattga agaagaaagt acacctgaaa
     6421 tcgccgctta atgctttgcg ggccgacgag aaatgtcggc ccgcattatt caggcacttt
     6481 cgcgaatggg ttcgatttca ttcaacgtat caattaacgg ctgcggctta ccaataagat
     6541 aaccctgcat ataatcgatc cccaaagaga gcaccgcctc gcggatctct tcgttttcaa
     6601 cgtactctgc cactacccgc attttcttca ttcgcgccag atggcaaatc gacgccacaa
     6661 tctgataatc cagactattt gacacaatat tgcggataaa gctgccgtca attttaagca
     6721 gatcggcatt cacattttta agccgtgcat agctggcgta gccggtgccg aaatcatcaa
     6781 tcgcaatctg gcagcctaat tcctgaagat gctgcaaggt gatttgcgcc tgcttaacat
     6841 tggtcagagc attactttcg gtgacttcaa aaataagttg ccacgcttca atctgatatt
     6901 tagccagcaa ctgactgact tcaacaggaa aacgagcctg acataccgag gttggagaca
     6961 gattaatagc aaaacggtga gcgggcatct tcgctctgtt ttcagccata aattgcagcg
     7021 tatgctcaat gacccacatg tcgatactcg acgataaacc aaattcgtgc gcgaccggca
     7081 agaagctatc ggggacgatc agttcatcat tctcaccttt catgcgcaga agaatttcat
     7141 ggtaaacatc accacgcata ccggtaatcg gctgggccat caggaaaaaa tggttgtgtt
     7201 ccagcgcctg ctgtagtcga ttcatcatcg cgactttatc tttcaattcg cgttgcaaat
     7261 acattgcccc gcgacgctgc atattttccg gggcgttggt cacgatggaa agttcggcga
     7321 ccgtatttag ctctcccagc agcaggtaga tatgattcac tggcgagcgc acatagcagt
     7381 aactgacgcc aatctgcggt tgcatcggca tcccatccca aaagaaacga aattgcttga
     7441 gatgactatc cagtgcggta atgcgctcct ggtgcgattc tgtattcagt cgcagcgcga
     7501 gatcgttacc cgaaagctga taaacatctt cacccggttc cagcaagggt gacagccagt
     7561 gagaaagttt ttgcttgtat tgaatccgca gcatgatgcc atagttctta accagcattt
     7621 ccatgccagg gatgcgtaaa taacaaagcg cagaccaggg agcatcacgc aacgcgcgat
     7681 tcagggcgcg aacatttggc agatgaacca ccgggtccat atacgcaagc cgctgaatgc
     7741 gtctgactac cgctctctga cgggttgcca gtacagccat ataattgaca ataaaagaga
     7801 ataccagata actggaggag gttatggtca gctgcgtggt atagccagga taaatgggaa
     7861 tgtaattttg atagctgtgg atgctgatca tcaacacgac cgcccagagc agcgaaatca
     7921 gcttataacc atagcgcatc gctccccaca tcatcagggg cagcaataat gacaaggtat
     7981 aattggtgct aaaaattgtg cttttttcat ttaacggcat gcataacagc aacaataaag
     8041 cacctaatgc cagtagccag agcgcgaact cttttttggt gactttggca tcaacctgct
     8101 gttttaattg cgaataatag ctacgtaaat aaaatggatt tcgcactacc cgaatgatga
     8161 agtagcacag cgggacaccg atcagattac ccaccagtaa ggcctgataa ttgattaaag
     8221 tcccgaggtt aaaaggcatg acaccgacca gattttctct gctcgccagt aatcctacaa
     8281 atgcagcaaa ctggaaaagt atcagaaaca gcgttgcagg aaacacaatc tgccagaata
     8341 tacgctggga aattaaacgg gtatcaccat gtgaaacgtt gttacgccgg ggagtaaaga
     8401 ccctgtaacc gccccaacag agaatgataa tgataataaa attagccgtt aatgatagcg
     8461 tttcgtaaaa acccagctct gcatatttgc ggacaaaaat ccctaaggcg atccctggca
     8521 atgccgccca gctgaaaaac atcatcatgc tgatcatcag tgccagaggc agataaaaga
     8581 gaaaaacctc accggaagaa atatgcgcga aagtattaat gtgggcgaaa atgggcaaga
     8641 ttaaagaagg caggaacagc ggaagccccc accatttatc acgcattttt atataagttg
     8701 cattcagttt catagatgct cagcagaatc ccccacatcc tgaaggaggt gtattcagac
     8761 aggcatccca cctgacttcg aatgatgatt attcatcact atagagagca ttgattctaa
     8821 gtgtcatatg aaagttccaa ttgatatata tcaaacaaaa taaccctgat taatgaatta
     8881 ttacgtttat catgttaatt catcattatt acatcatcat tgtaaataat taaattaact
     8941 tccataacat taaaatatgt atccactgac gtttttttac ataacgaaga attgaccatt
     9001 ttgtcctgtt gtgccttaat gtaagtaccg tccacagcgt gggacatact tcaaggaacc
     9061 ttttgtgagt caggcaacca gtatgcgaaa acgacaccga tttaacagtc gcatgacccg
     9121 tatcgtactg ctcatcagct ttatcttctt ctttggccgt tttatctact cgtccgtcgg
     9181 tgcctggcag caccatcaga gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc
     9241 accggtacaa cgttagcggt taccttcttc actttcacag aacataacgg cacttcgctg
     9301 tcggatgctt ttgctctttg ggattatcaa agcggcagat attctttcat cttaaatttt
     9361 acgtctttgt cctgactgat gtttatcctg tttggctgcg gaataaatat aaaattaata
     9421 tatatgttgt aatgatatat ttttataaat tattccctgc gtgaatttta ataaatttaa
     9481 tctatccctt tatacgcaat acatttactt tcctcttttg atgatcttaa atgtcttatt
     9541 tttcgtaatg tgtataacaa ggaatagtga tgaaatttaa aaaatgtctt ctgcctgtgg
     9601 caatgttagc gtcattcact ctggcaggat gccagtcaaa tgctgacgat catgccgccg
     9661 atgtttatca aaccgatcaa ctgaatacca aacaagaaac taaaaccgtt aatattattt
     9721 ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa taaacggaac gcacaagcct
     9781 tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg ccacaacgtc ggttctggca
     9841 gcaattccgg aacgacggca ggggcagttg gcggcggagc tgtaggcgcg gcagcgggtt
     9901 ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt aacatataag gaaggcacca
     9961 aagtgtatac ctccacccag gtgggtaaag agtgccagtt tacgacaggt ttagccgttg
    10021 ttattaccac aacgtataac gaaacgcgta ttcagccaaa tactaaatgt cctgaaaaga
    10081 gctaataatc aggaggagtc atgaagaaag tttttctttg cgccatctta gcctccttaa
    10141 gctatccggc tatcgcctca tcattgcagg atcaactctc tgctgtcgca gaagcggaac
    10201 agcaaggtaa aaatgaagag caaaggcagc atgacgaatg ggtcgcggag cgcaacaggg
    10261 aaatccagca agagaagcaa cgtcgcgcaa atgcccaggc cgccgctaac aaaagagcgg
    10321 caacggcagc ggcaaataag aaagctcgtc aggataaact ggacgccgaa gccactgcgg
    10381 acaaaaaacg cgatcaaagt tatgaagatg agctacgcag cttagagatt cagaaacaaa
    10441 aactgacgct ggcgaaagaa gaagcccgcg ttaagcgaga aaacgaattt atcgatcagg
    10501 aactgaagca caaagctgcg caaaccgatg tggtgcaatc tgaagctgac gccaacagaa
    10561 atatgactga aggcggtcgc gatctgatga aaagcgtggg caaagcagaa gagaacaaat
    10621 cggacagctg gtttaattaa tcgatgttag taacttcaag cctatgattc ttgaagataa
    10681 aaaaccctct gtagtaacag agggttttgt tcattcatag tgcagggatt aaaatcattc
    10741 ccactcaatg gtagctggcg gcttgccgct gatgtcatac accacgcggg aaataccgtt
    10801 cacttcattg ataatgcggt tggaaacgcg accgaggaaa tcgtacggca gacgcgccca
    10861 gtgtgcggtc ataaagtcga tggtttcgac agcacgcaga gagacaaccc agtcatactt
    10921 acgaccatcg cccattacgc caacggaacg taccggcagg aacacagtga acgcctggct
    10981 gactttgtcg tacaggtccg ctttacgcag ttcttcaatg aagatggcgt cagcacggcg
    11041 cagcaggtca cagtactctt tcttcacttc acccagcaca cgaacgccaa ggcctggtcc
    11101 cgggaatggg tgacggtaca acatgtcgta cggcaggccc agctccagac caatcttacg
    11161 cacttcgtct ttgaacagct ctttcagcgg ttcaaccagg cccatcttca tctctttcgg
    11221 caggccgccc acgttgtggt gagatttgat gacgtgtgct ttaccggttg cagacgccgc
    11281 agattcgata acgtcagggt agatggtgcc ctgcgccagc cacttcacgt cttccagttt
    11341 cagcgcttct tcatcgaata cttcaacgaa aacgcgaccg atgattttac gttttgcttc
    11401 cggatcgttt tcgccagcca gcgctgacag gaagcgatct tctgccggaa cgtgaacaat
    11461 gttcagacca aagtgatcgc caaacatatc cagaacctgc tctgcttcgt tgagacgcag
    11521 caggccgttg tcgacgaata cgcaagtcag gtttttaccg atagcgcggt gcagtagcat
    11581 tgcggttacg gaggaatcca cgccaccaga gaggccgagg atgactttat cgtcgcctac
    11641 ctgctcacgg atgcgggcta cagcatcgtc gataattttc gctggcgtcc acagggcttc
    11701 acactggcag atatcacgca caaaacgctc cagcatgcgc ataccctggc gggtgtgagt
    11761 cacttccggg tggaactgta cgccatagaa gcgtttttct tcgttagcca taatggcaaa
    11821 cgggcagctt tcggtgctgg ctacggtgac gaagtcggac gggatagcgg taactttatc
    11881 gccgtggctc atccagacat cgagcagcgg tttaccgtct gcggtcagcg catcttcgat
    11941 accgcgaacc agtgcactgt cgtttacgac ttcaacctgc gcgtagccaa attcacgttc
    12001 gttagaggct tcaacgtgac cgcccaactg cattgccatg gtctgcatgc catagcaaac
    12061 gccgaatacc ggtacgcctg cttcaaagac atactgcggc gcacgcgggc tgttttcttc
    12121 agtagtactt tccgggccgc cggaaagaat aatgccgctt ggattgaagt cacgaatttg
    12181 tgcttctgtc acatcccacg cccacagttc gcagtaaaca cccagctcac gcacgcggcg
    12241 cgcaaccagt tgagtgtact gagaaccgaa gtccagaatg aggatgcgat gcttatgaat
    12301 gttttccgtc attgacgctt attccgaggc aagtgaaaca gataatataa atcgcccgac
    12361 atgaagtcgg gcgaagagaa tcaggagccc agacggtagt tcggggactc tttagtaatg
    12421 gtcacgtcgt gaacgtggct ttcctgaatg cccgcaccgc tgatacgtac aaactccgct
    12481 ttagtacgca gttcgtcgat agtaccacag ccggtcagac ccatacagga gcgcaggccg
    12541 cccatctgct ggtgaatgat ctctttcagg cgacctttat aagctacgcg accttcgata
    12601 ccttccggca ccagtttgtc ggcagcgtta tcgctctgga aataacggtc agaggaacct
    12661 ttggacatcg cgcccaggga acccatacca cggtaagatt tgtaagaacg gccctggtag
    12721 agttcgattt cacccggaga ttcttcagta cccgccagca tggaacctac catcaccgcg
    12781 cttgcgccag cggcgatagc tttggcgatg tcgccggaga agcgaatacc accatctgcg
    12841 ataaccggaa taccggtgcc ttccagggct tcaactgcgt cagcaacggc agtgatctgc
    12901 ggaacaccta cgccagtaac gatacgagtc gtacagatag aaccagggcc gataccgact
    12961 ttaaccgcgc tgcaaccagc ttctgccaaa gcgcgtgcac ctgcagctgt tgccacgttg
    13021 ccgccgatga tttgcagatc cggatattta gcacgggttt cacggatacg ctgcaaaacg
    13081 ccttctgagt gaccgtggga ggagtcgatc agcagaacgt caacgccagc ggcaaccagc
    13141 gcgtcaacac gctcttcgtt acccgcacct gcgccaactg cagcaccaac acgcagacgg
    13201 ccttgctcgt ctttacaggc gttcggttta cgttccgctt tctggaagtc tttcacggtg
    13261 atcatgccga tcaggtggaa ttcgtcatca accaccagcg ctttttcaac gcgtttttcg
    13321 tgcatttttg ccagcaccac ttcacgggct tcaccttcac gcacggtgac cagacgctct
    13381 ttcggcgtca tgtaaacgct aaccggctgg ttcaggtcgg taacaaaacg cacgtcacga
    13441 ccggtgataa tacccaccag ttcgttttct tcggtaacga ccggatagcc cgcaaaaccg
    13501 ttacgctcgg tcagttcttt cacttcgcgc agcgtcgtgg ttggcagaac agtctgcgga
    13561 tcagtcacca caccagattc gtgttttttc acacggcgaa cttcttctgc ctggcgttca
    13621 atggacatgt ttttgtggat aaagccgata ccgccttcct gagccagagc aatagccagg
    13681 cgcgcttccg ttacggtatc cattgctgcg gaaagcatag ggatattcag acgaatagtt
    13741 ttcgtcagct gggtgctgag gtcagcagta ttcggcagaa cggtagaatg agcaggaacg
    13801 aggagaacgt cgtcaaacgt cagagcttct ttagcgatac gtagcatggg caatatctcg
    13861 accagagtgg ttaataaata ttgccgcggc attatacaga gcgtaaccga ttgcatctac
    13921 ccctttttgc aaaaaatgct tgctatcccc gaagggcggg ttactatcga ctgaataacc
    13981 tgctgattta gaatttgatc tcgctcacat gttaccttct caatcccctg caatttttac
    14041 cgttagtcgc ctgaatcaaa cggttcgtct gctgcttgag catgagatgg gacaggtttg
    14101 gatcagcggc gaaatctcta atttcacaca accggcttcc ggtcactggt actttacact
    14161 caaagacgac accgcccagg tacgctgcgc gatgttccgc aacagcaacc gccgggtgac
    14221 cttccgtcca cagcatggac aacaagtttt agttcgcgcc aatattacgc tctacgagcc
    14281 gcgcggtgac taccagataa tcgttgagag tatgcagccg gccggtgaag ggctgctgca
    14341 acagaagtac gaacagctca aagcgaagtt gcaggctgaa ggtttgttcg atcagcaata
    14401 caaaaaacca cttccctccc ctgcgcattg cgttggtgtg atcacctcaa aaaccggtgc
    14461 tgcgctacat gatattttgc atgtgttaaa acgtcgcgat ccttctctac cggtgatcat
    14521 ctaccccacc gccgttcagg gcgatgacgc accggggcaa attgttcgcg ccattgaact
    14581 ggcgaatcag cgcaatgagt gcgacgtgtt gatcgttggg cgcggcggcg gttcgctgga
    14641 agatttatgg agttttaacg acgaacgcgt agcgcgggcg atttttgcca gccgcattcc
    14701 ggtcgtcagc gccgtcgggc atgagacgga tgtgaccatt gccgattttg ttgccgatct
    14761 gcgtgcgcca acgccgtctg ccgccgctga agtagtgagc cgtaatcagc aagagttact
    14821 gcgccaggtg caatcgaccc gtcaacggct ggagatggcg atggattatt atctcgccaa
    14881 ccgcacgcgt cgctttacgc agatccatca ccgattacag caacagcatc cgcagctccg
    14941 gctggcacgc cagcaaacca tgcttgagcg cctgcaaaag cggatgagct ttgcgctgga
    15001 aaatcaactt aagcgtaccg ggcaacagca gcagcggtta acacagcggc tgaatcagca
    15061 aaatccacag ccgaagattc atcgcgcgca aacgcgcatt cagcaactgg aatatcgttt
    15121 agcagaaacc ctgcgcgtac agcttagcgc cacgcgtgaa cgtttcggta atgcagtaac
    15181 gcacctcgaa gccgtaagcc cactgtcaac gctggcgcgt ggatacagcg ttactactgc
    15241 tactgacggc aatgtactga aaaaagtgaa gcaagttaaa gcgggtgaaa tgctaaccac
    15301 acgtctggaa gacggctgga tagaaagtga agttaaaaac atccagccag taaaaaaatc
    15361 gcgtaaaaag gtgcattaag ccagcacgaa ttcaacgcgt tttttcgata tcaagccatg
    15421 cccgtgctga cagaagtaat ctactgcacc gcaggctttt aacacctgca acggttgatg
    15481 gcagtctgga caaagagctt tcatttcaat aatttccccg caggatgggc aacgcgcatg
    15541 gccgttatcc tgatcaagca catgctgaca ttgtggacaa tgcagttcca tatgaattcc
    15601 tccggtagcc atccctctac attcatagag ggaatggcag ataaaatact tacggataat
    15661 tatttatttt tcttgatgtg cttcatcaga cgcttacgtt tacgcatctg ggttggcgtc
    15721 agggtgttac gcttgttcgc atacgggttt tccccttctt tgaactgaat acgaatcggc
    15781 gatcccatta cgtccagcga tttgcggaag tagttcatca agtagcgctt gtaggaatca
    15841 ggcaggtctt tcacctgatt accgtgaatc accacaatcg gcgggttata accaccagcg
    15901 tgggcatatt tcagcttcac acgacgaccg cgtaccagcg gcggttggtg atcttcaaca
    15961 gccatcgtca tgatgcgcgt cagcatagag gtccccacac gacgggtgga gctgtcatac
    16021 gcttcacgta ctgattcaaa caagttacca acaccactgc cgtgcaaggc agagataaag
    16081 tgcacacgag caaaatcgat aaagcccaga cggaagtcca gcgtttcttt cacctgctct
    16141 ttcacttcct ggctcaggcc atcccactta ttcaccacaa tgacaagtga gcgcccacta
    16201 ttgagaataa agcccagcag cgagagatcc tgatcggaaa taccttcgcg cgcatcaatc
    16261 actaacatca ccacgttggt atcttcaatg gcctgcaacg ttttgattac ggagaatttc
    16321 tctacagcat cggtgatttt gccgcgttta cgtacgccag cggtgtcaat gagcacatac
    16381 tcacgtccat cgcgttccat tggaatgtag atactgtcac gcgtcgtgcc aggcatgtca
    16441 taaacaacaa cgcgctcttc accaagaata cggttagtga gtgtagactt acctacgttc
    16501 ggacgaccca caatcgccag tttgatcggc agactttgcg ggtcgaagtc gtcttcctct
    16561 tcttcttcgc cgttctcttc cgcttcaaat tgcgcccagt attcagcgtc ttcgtcgact
    16621 tcctcttgcg gtgcgagatc ttccatccac ggcagcagca cacgctccag cagacttaat
    16681 acgccacgac cgtgagatgc ggcgatcggg tagatttcac ctaaaccaag cgagtagaaa
    16741 tcaaccactg cctgatcggg atccagaccg tcagttttgt ttgccaccag gaaggtcggt
    16801 ttttcacgag agcgcagatg tttggcaatc gcttcatctg ccggcatcaa gcccgcgcgc
    16861 gcatccacca taaacagtac gacgtccgct tcttcaatcg ccagcagcga ctgttccgcc
    16921 atgcgggttt ctacaccgtc ttctgtgcca tcaatcccgc cggtatcaat acagataaac
    16981 tcacggcctt caatttccgc acgaccgtac ttacggtcac gagtcagacc cgggaaatcc
    17041 gcaaccagcg catctcgggt gcgagttaga cggttaaata acgtggattt tcctacgtta
    17101 gggcgcccga caagcgcgac cacaggtacc atgtttaaag cctcattttt ataaatcatc
    17161 agacaacgca cgctatattc gcgtcgttgt taaaaacagg aaaacggccc ctgtccagga
    17221 gccgttttca aagtgaacga cagagacgat taacgtgtaa tagagtacac ggttccgtct
    17281 tttgcctgga tcagcagttt gccgtcagcg gcaaccggtt cagtctggaa accggaacta
    17341 tcaacttttt gctgggcaac gaaacgacca tcttcgacgt taatccagtg cagataacct
    17401 tcactgtcac ctaccaccag gttgccatta tacagcaccg gcgaagtcag caggcgatgc
    17461 agcagatcgc tttgtgtcca cagcgtaacg ccgccatcaa tggtcaacgc catcacccgg
    17521 tcattttgat cgaccagata gatgcgattg ccgtcgacga tgaaatcatt caccgaaccc
    17581 agttcgcgtt tccacataat ctgaccactg cgcagatcaa gcgccgtcag gttaccatta
    17641 taggccagag cgaaaacaac gccgttaaca acgacgggag tcgtgtcaac atcgctcaga
    17701 cggtcaattt cggtagaacc ggtcgcctgg gaaatacgct gctgccaaat catctggccc
    17761 tgttccatca gcactgcgct gacgcgacca ttatcgcccc ccacgacggc cgcaccaaaa
    17821 gccgttgccg gcgcagactc gccacgcaaa gagagcgaag gcatatcgag gttaactgtc
    17881 catttgacag cgccgtcagc ttcgttcagc gcttgtaact gaccgttact agtgtggatt
    17941 aacaccagac cgtcgctgac caccgggcga gaaagtgctt cacccgcgac tttagtttgc
    18001 catgccacag taccatcgct ggtattcagc gcgtaaacct gcgccttttc gctgccaatg
    18061 tagacatgcc caccagacac ggtcacaccg ccagaaagta atgcaggctc tttagagaac
    18121 cagccatctt tctcggccag gctgacagac cagatttctt tgccatcatc cgcattcagc
    18181 gcttttacca aaccagcgcg gtccgctgca tagacaacgt tgtccgccag tgccggatga
    18241 agattggaat agaagttgcc aatgccgcta ccaacggaag tgctccacgc cgtggtcggc
    18301 gtaaactggt tttcaacggt tggcaatggg gacatcttta ccacatcttc ttcgctgtta
    18361 aacagcgaac agccgcttaa aagggtaacg gaaagcagtc ctggcagcag taatttacgc
    18421 aattgcatcg ggtccctctc agatggacaa attattaatt ttcatctgca tcatttcgct
    18481 cagtgccgga gtaacatcgc ttttcacgcc tgcttcccat gcactacgcg caccttgctt
    18541 atcacctttg ctcaacaatg ctttaccacg caggtcggca acaatggcag cccacccttc
    18601 acctttgatg gtatcaaggg ttttcagcgc ggcatcagcc tgcttgagct gtacctgaac
    18661 gcgagcaaga cgcagattta tcacggcttt gagattttca tcactcgtgt ctgccagccc
    18721 ctgttgtaac tgggcggcag ctttctctag ttcatttttg tcaacaaatt gctgcgcaag
    18781 ttccaaagaa gccagcgcac cataagtatt tttattttca gcagcaaatt tttccgccgc
    18841 cgggatgcta tccggtttgc cttcgctcac tgcggtaacc gcattttgat aggcaagaga
    18901 agcggagcgt gcagaatcaa cctgatggct gttccagtag cgccagccaa tcagtgcgcc
    18961 aacgcccaaa atcaccccaa cagccagtgc tttgccattt tcagcaaaaa agcgtttaac
    19021 cgcttctacc tggtcgtttt cgttctcgta aatttccacg ctgtccttct ccttccttaa
    19081 cccagtaacg tgcgcaaatg cgcggctacg ctatcctgcg caactgccgt ttgctcacca
    19141 gagcgcaaat ccttcactac tgctgtgccg ttagccactt cagactcacc cagcaccaca
    19201 gcaacgcggg caccccattt atcagcacgg gcaaactgtt tcttaaagtt gccgccgccg
    19261 tggttggtca tcaatttcac gcccggtaat tcatcacgca gacgctcagc taatgccata
    19321 gccgcagatt gtgtatcagc acctgaagcc accaggtata tatcgacaac aggatcggct
    19381 ttaaattccg gattaacggc ctgtactaac aatacaagac gttcgaggcc catcgcaaaa
    19441 ccgactgccg gtgttgcacg accgcccagt tgttccacca gaccgtcata acgaccgcct
    19501 gcacacacgg tgccctggga gccgaggctg ttagtcaccc actcgaaaac ggtacggtta
    19561 tagtaatcca gaccacgcac cagacgctgg tttacggtgt aagcgatccc cgcgctctca
    19621 agcagtttgc acagaccggc aaaatgctca cgagattcct cgtccagata atcacctaat
    19681 gccggagcgt cgttgagaag cgcctgcact tccggatttt ttgaatccag cacgcgcagc
    19741 gggttagtgt acatgcggcg tttgcagtct tcgtccagct tttctttatg ctgctcaagg
    19801 aatgccacca gcgcatcgcg gtaattggcg cgtgcttcca gcgaaccaat agagttcagc
    19861 tcaagagtta cgtgttcgaa gatacccagc gcacgccacc agcgggcagt gagcataatc
    19921 agttcagcgt cgatatccgg accttgcaga ccgaaaactt cgcagcccaa ctgatggaac
    19981 tgacgataac gccctttctg cggacgctcg tgacggaaca tcggcccgat ataccacaga
    20041 cgctgttcct gattgtacag aagaccatgc tcgatgccgg cgcgtacaca gcccgccgtc
    20101 ccttcagggc gcagagtcag gctgtcgcca ttgcgatcct caaaggtgta catctctttt
    20161 tcaaccacgt cggtgacttc accaatcgca cgtttgaata gcggggtctg ctctacaatc
    20221 ggcaagcgga tttcactgta accgtagctg ccgagcacgt ttttcagtgt gccttcaatg
    20281 cgctgccaga tggccgtttc gccaggcagg taatcgttca tgccgcgaat ggcttgaatg
    20341 ttttttgcca cgtttattct ctttctgaat ataaaaatga accctcaacg cttccctcaa
    20401 tgtttcggga gccatgcggg ttcaatcata cacgggaagc gaggcgcttc ccatcacgtt
    20461 attatttttc aacctgctga acgtcaattc gacgcgcttc gtccagctga ctggctttcg
    20521 cacgaatgcg tgcttccagc tggtcgatca tatcgttgtt gtccagacgg tctttgcgca
    20581 cgccatcttc atagaggccg cttttcttgt tgccgccggt gacgccgagt gtagaaacca
    20641 gcgcctcacc tgggccattc accacgcagc cgataatcga aacgtccatc ggagtgatga
    20701 tatcttccag gcgttgctcc agcgcgttaa ccgtaccgat aacatcaaat tcctgacgcg
    20761 aacaggtcgg gcaggcgatg aagttaatcc ctcgcgaacg gatacgcagc gatttcaaaa
    20821 tatcgaaacc gactttgatc tcttcgaccg gatcggccgc cagcgatacg cgcagcgtgt
    20881 cgccgatgcc ttccgacagc agcagaccta aaccaatggc ggattttact gccccgctgc
    20941 gtgcaccacc ggcttcggtg atccccagat gcagcggctg atcgatctgt tttgccagca
    21001 aacgataaga ctcaacagcg aggaagacgt cagacgcttt cacgctgact ttgaactgat
    21061 cgaagttcag gcgatcgaga tgatcaacat gacgcatggc ggattccagc aacgcctgcg
    21121 gcgtcggttc gccatacttt tcttgcagat ctttttccag cgatccggcg ttaacgccaa
    21181 tacggatcgg aatgttttta tcgcgcgcac agtcaaccac catgcgaata cgctcttcat
    21241 taccgatatt gccagggtta atacgcagac aatcgacgcc gtattccgct actttcagcg
    21301 caatgcgata gtcgaagtgg atgtcagcca ccagcggcac gttaacccgc tgtttgatga
    21361 gtttgaacgc ttctgccgcg tccatcgtcg gtacggagac acggacgata tcagcgccaa
    21421 cgcgttccag cgccttgatt tgattgaccg ttgcttcgac atctgtcgta cgcgtattgg
    21481 tcatggactg tacggcgatg ggagcaccat cgccaatcgg cacattccca acgtaaatac
    21541 gtgttgattt tctacgttga attggagcct ggttatgcat gaaaaatctc ccgcgttacc
    21601 cgtctgttac tgcgccggtg attgttcggc attgagggtc agacgcgcaa cctggttagt
    21661 tctgataaaa cgactcagat cgacaggttt cccttgatac tggatctgta ctgcggctgg
    21721 cgcaccaatt ttcagtttat acggtgcctg gccggttaaa ttcaaattac cgtctttacg
    21781 ctgcatcccg ctaaacaatt ttttaccggt agcatcagtg acctccagcc agcaatcggc
    21841 agtaaagttc atcaccagcg cattcggatc agccgccggc gtggtcacgc cagcctgatc
    21901 ggttggcaac ggcgcagcac catctggcgt tgttgctgcc gttggtaccg gggtcgcggc
    21961 ggtatcaaca tttgcctgcg aaggcgaaac aaccgcattc tgttgcggat caacagctgg
    22021 tgctggcgca gttacggcag gtgtttgcgt gttggttgcg gtagtatcca cagacgctgg
    22081 cggcgtggac gttgtagccg ggtctgtagt tgtcgacgta tttaacggga cactctgccc
    22141 ctgctcgcta ttactgctca gttctgccga agattgatcg gccatagtgg tgatctcttc
    22201 ctgctgagct ttgtggtctt gccaccacca ggcaccgctc aggccgataa ccacaaacaa
    22261 caccagccag gtgaaggtca tcagccagcc atcacgtttt ttgcggcgtt taccgaggga
    22321 aaaactctgc attggcgcaa cttttgcagc ccgtagtgga gcctgctttt ccagccctgg
    22381 cagcagttct tcttctggaa tatgtaccag acgcgcataa gagcggatat atccgcgcag
    22441 gaatgttgaa gcaagatcgg cgggtgcctt atcttcttca atgtcgcgta ccgtggaaac
    22501 cttcaggcaa agtcgctcgg caacggcctg ctgactaagt cctagttgtt cgcgagcatt
    22561 acgcaggcga gcgccggtag taagtgcttc attttggtcg tgcgtggctt cagtattcat
    22621 tcgctgcagg tacgtttaaa tgagaattga gatgccggtg aaccatcatg ctcacccaca
    22681 ccgcgaaaca tccgttaagt taaccgttat catacagtat aagaccgtct gtgtgctcgt
    22741 gacaaagccc acattcacta aggctaactt actgttgcat cgttacatac tgccttaaag
    22801 tcagcaaaaa cgcaccgtta atattgacca gacaattgca acttaatact tcattcataa
    22861 aacattacgc cacggtacat aaagtaaccg tggcgtaatg gctatcagac cgctttaatg
    22921 tcgatggctt caccctgcat ccgtttacgc agggtacgtt tcgtacggtc gataacatcg
    22981 cccgccaact gaccacaggc agcatcaata tcatcaccac gagttttacg cacaatagtg
    23041 gtgaaaccgt agctcatcag cacttttgag aaacggtcga tacggctgtt cgagctgcgt
    23101 ccatacggcg cacccgggaa cgggttccac gggatcaggt tgatcttaca cggcgtatct
    23161 ttcagcagtt ccgccagttg gtgcgcgtgt tcagtgccgt cgttaacgtg gtcaagcatc
    23221 acgtattcaa tagtgactcg gccctgattg gcgttggatt tctccagata acggcgcacc
    23281 gcagcaagga acgtttcgat attgtacttt ttgttgatcg gcacaatttc gtcacgaatt
    23341 tcgtcgttcg gcgcgtgcag ggaaattgcc agtgcaacgt cgatcatatc gcccagttta
    23401 tccagcgccg gaactacacc ggaagtagaa agcgtgacgc gacgtttaga caggccaaaa
    23461 ccgaaatcat caagcatgat ttccatcgcc ggaacgacgt tgttcaggtt gagcagcggc
    23521 tcgcccatgc ccatcatcac tacgttagtg attggccgct gaccggtgac ttttgctgcg
    23581 ccgacgattt tcgccgcccg ccacacctgg ccgataattt ccgacacccg caggttgcgg
    23641 ttaaagccct gctgggcggt ggaacagaat ttacactcca gcgcacaccc cacctgcgaa
    23701 gagacgcaga gcgtggcacg gtcgtcttcc gggatataca ccgtttcgac gcgctgatcg
    23761 ccaacggcga tcgcccattt aatggtgccg tcggatgaac gctgttcttc aaccacttcc
    23821 ggtgcgcgga tctccgccac ctctttcagt ttgccgcgca acactttgtt gatgtcggtc
    23881 atctcatcaa agttgtcgca gcaatagtga tacatccact tcatcacctg atcggcgcgg
    23941 aagggttttt cacctaaatc tttaaaaaac tcccgcatct gctgacggtt gagatccagc
    24001 aggttgattt ttccatcttt cgtggtgacg ttttcaggtg tgactaattg ttcagacata
    24061 tgctattccg gcctcgttat tacacgttat ggcccctgga gggttgaaaa aagaaacgcc
    24121 ccggtgagcg gctgctcgtc cgggggcgct gcattgtaca aattctggcg cacggatgcc
    24181 acgtttgcac gcggcattta cgaaattatt aacgggtgcg cgggcacact tcgccttcgc
    24241 cgaagaaata agcgatttcg cgagcggcag attcgacgga atcagaaccg tgggtaccgt
    24301 tttcggtcag gctgtcagcg taatcagcgc gcagagtacc ggccagtgcg tttgccggat
    24361 tggtcgcgcc cagcagatcg cggtgacgct gaacggcgtt ttcaccttcc agcacggaaa
    24421 ccacgatcgg gccagaggtc atgaattcaa ccagaccatc aaagaacggt tttccatcgt
    24481 gttcagcata aaagccacga gcctgttcaa cggtcaggtg cagcattttg gtgccgacaa
    24541 ttttgaaccc tgcagcttca aagcgcgcaa agatattacc aatgacgttt tttgctaccg
    24601 cgttcggttt gatgatggaa aaagtacgtt caatagccat ttttacctct gtaaattgtt
    24661 ctgttgttgt ctgtaccagc gtacgagatg gcgcggatta taatgagcaa cagggccgtt
    24721 gactattgat gaaggtaaca tttttttaaa ataaaatgag ttttagcaac aatcagggac
    24781 aaaacagact attgcatgac aaatttcact gtcgcgattt gccccacgtc atccatcacc
    24841 agcaattgat aatcaccttt atccgtcaaa tgcagggtca cgttgcgccc gcgttcagtt
    24901 aacggttcgc cattcagaaa ccaccagcgt tcacctgccc ctccacttga ttgcaacggc
    24961 aaagtcgctt ccgctgcgcc cggtaaacgt ttaataatcg cgccatcgcg aacgcctgtc
    25021 agctgcagag ggagctgggc atcgtgaccg tatggcgggc aaattgtcga ggccggtggt
    25081 aagcgcacag cgcgacgttc tgatgcgggc agccagggtt ccagcggtag cggccagaca
    25141 tttaccattt cttgcttcgc ctgcgggcag tcagcggcga cgcgctgccc tttgtcatcc
    25201 agccagatgg ggaagcgaat gccattaatc ccttcctgct ctgacagtaa cagcgttggc
    25261 ggctggctgc catccagcag ccaggttgcc aggcggcggc gacagttacc gtcaccttcc
    25321 ggcaaagact gtccgcccgg ccagcagata acgccacgag tgactgagtt cgggcgcggg
    25381 tcttccggca gattcgcact gcgcgacagt aagatattat tgacctgatt caacaatggt
    25441 acggcactgg caaagccaaa ctgaccaaca acgggcgtgc cgtcaggtct gccagtccag
    25501 atcccaatga catagcgagc gttaacccca atcgcccagg cgtcacgata gccatagctg
    25561 gtgcccgttt tccatgccag tggggcgacg cgcggcaagg cactatccgg caagggttgc
    25621 gcttcatcag ccataatccg gcgaatgatc cacgccgccc ccgacgacat taaaggccgt
    25681 tcaagcagcg gatcgtcagg ctgtaagcgc aatttgcctg ccttgccgtg gcgagcaaac
    25741 gcggtatacg ctgccgccat atcttccagt tttgcaccag cgccgccgag aatgagtgaa
    25801 agattcggcg cagcgccgtt gggcaaatat aacggcaatc caacattgcg taactttgcc
    25861 gcaaaccgtt tcggtccata ggcttccagc acctgcacag caggtaagtt cagcgagcgc
    25921 accagcgcct cgctcatgct gatcgggcca tgaaaaccgc tatcaaagtt acctggtcga
    25981 taatcaccgg tgcgccgggg gacgtcttgc agcagtgatg ccgggtggat caagccttca
    26041 tccagcgcca gaccataaac aaacggtttg agcaccgatc ctggcgatcg gatcgcattg
    26101 accatatcaa catgaccaaa gcgtgaatca tcgttgagat caaccgatcc cacccagccg
    26161 cgaacacgca tatcggtatg atcaaccacg atcatcgcca gtgagctgcg cggtggcaac
    26221 cgccctttcc agttttgcgc cagttcttcc agacgtcgtt gaagaccggc atccagcgta
    26281 gtaacgattt tgtcgctttt gcttttaccg agcatcatgc gcgaaaacag cggtgccagt
    26341 tgcggcattt gtcggggtgc cagccagatg ggttcttccc ttgactcttt tacctgctca
    26401 cgggaccaca caccttgcgc agccatccgt tcgagcactt tattacgcgc ggcttcggca
    26461 cgctccggcc aacgatccgg gcgaagacgg ctgggcgctt gcggcaaaac cgccagcatt
    26521 gccgcctcgg aatagcttaa attcgcaggc gactttccga gataagccca acttgccgca
    26581 ccgatcccct gcaacgtacc gccaaacgga gcgcggttaa gatacaaggt cagaatttca
    26641 cgcttagaca gatgccattc cagttgcaac gcgcgccaga gctggcgaat tttgccgcca
    26701 aatgttttgg ggtgaggatc aagcagacga gcaacctgca tagtgagcgt gctgccaccg
    26761 gaaataaccc gtcccgaagt gagatcttgc catgctgcgc gcgccaccga gaatggattc
    26821 acccccggat gcttccagaa ccagcgatct tcataattga tcagcgcttc aaggtaacgt
    26881 ggagaaacat cttcgattgt taccggataa cgccagatgc cgtcagcatc ggcgaagcgc
    26941 cagagcggcg taccatcctg cgccacgacc actcgtgcgg gattgacttc atgcagcggt
    27001 agaggccaga gtttatctgc tccccacgct gcaagaaaaa ggagaaatgg cgcagctgcc
    27061 agcgttatcc agcagccgcg tttggttaac aagcgaggca tttacggtcg gacaatcagc
    27121 agatcttcag ccgcgccggt cgcccgccat tggggaacat acattgattc caccatcggt
    27181 tgcggtacct gatacgttcc cggcgtcacc gcccgcgcca gatacaccag cgttaccggt
    27241 tggtattcat caacggcaac cgccgccaca aagcgatcgt cacggaactc aatgtgctta
    27301 atactcgcct gctgcatctg gttcagtaag ttctgcactt cgccaccact ttgctccagg
    27361 ctggcgctac cgttcgccag attctggttt tccagttcca gacccacagg cagcagatcc
    27421 acgactaacg catccggcac gctgttactg gcttttacct gcaaccacac cagcaccaga
    27481 tcgccgctac gtaacgagtc cagcgatttg ctcttaccat cagtaccaag aatatgacgc
    27541 tcgatttgca gcacattgtt cgccggtaaa ggtgcggatt gcggataacc gctggcatcc
    27601 atacgcaacc ataacggctg atcgccactg ttgctcacct gcaaggtgac aagttgatcg
    27661 ctattcagat tgctgttttg cgctttctcg cctgtcagcg gctcagctga gaaagaggtt
    27721 tgcgcctgcc atttacccgg taaatcctga atcgtacggg cagccaggaa caacgcgtta
    27781 ctttcctgcg tcgatagcca gcgttcacca aacgcctgct gcgaaagtgt gttcagcaaa
    27841 gtgtactgct catcgggtag cagtttattt tcttccagca aggagagcat taacgcgttg
    27901 tcgcgcagtg gactaccgta atcacccagc catatccgct catcactatt tctcggcgtt
    27961 ttcagcgcca gcgcaatcgc ttcttcacca cgcgtcgcat cacccatggt tttcagcgca
    28021 atgccaagtt gcagcagcgg taaaccagaa gcggcatctg cacgatgctc ccagatttca
    28081 cgcagcgcac ccagtggtgc tttttgctga cgggccagca ccagcgcagc gtaagactga
    28141 acggcgaatt tactggcttt gagattatcc gcgtacggga tcgacatcat gcccggatct
    28201 tgtaaatagc gcagcagacg ctcattaccc cggttaatgg cgtctgtcgg cacgctgtaa
    28261 ccctgttcgc ctgcgcggac caggaaatcc atcacgtaag ccgtcagcca gtactcttcg
    28321 tcaccgtttt tatcccacag cgcaaagccg ccgttatcac gttgcatttg cagcaaacgg
    28381 gaaatgccga tatcgaccga tgcacggcgt ttctcatcac tgtcgccttt gatgcccaac
    28441 gcctgcagtt gggctgcgtt ggtataaagt gacgggaaca ggccgctggc ggtttgctca
    28501 agacagccgt acggatacgc ttttaactct ttgatataac gtgcaatgtt cagcggtggt
    28561 ttgccgctca acaacaattg cccttccagc gtaacaggcg agaagttttg caatccatcc
    28621 gccggaatcg cccatgtctc accaggctgt aacgccgtac cgtaattaac cgtttgtgcc
    28681 gggaacgccg gacggacgcc gattttccac tgcttatgct gatcggcaac ggtttcaccc
    28741 ggtaacgcta acccgctaat ggtggcctga atttctccat cgccataacc cggcaatgct
    28801 cgcaccggga taaacagcgt agtacgcaca cctggcgcta attcaacggc tgcgggtgaa
    28861 tcgctgacca gttcaagcaa accactggcg gtcagggcaa cgttcagttt ttgcggttta
    28921 tcggtaagat tagtgatatc cagcgtcaga cgcgaggtat cgccactcgc cataaagcgc
    28981 ggcatgttca gttcagcaat caccggtgcg gcaactatca ctttgctttc gttgctaccg
    29041 aaatcatctg ccgtccaggc ttgcgccatg acgcgcaatt caccgttaaa atcgccaatc
    29101 ggcagtgtaa ccgagccttc gccctgttcg ttgagcgtta ccgacagcgc ctgctgcgcg
    29161 acaatattga cgtgattgac cggcggttta ccaccacgtt tcagctcatc accatcgcca
    29221 ccgaaacgca gagctgccag acgcccctga ccttcaataa cctgaccgta aatatcgtaa
    29281 atgtctgcgc catagcgttt ctgaccaaag aacgcctgcc acggatctgg cgtaacgtag
    29341 tcagtaatat tcagcacacc actatcaacg gcagacacca gcacattcac ctgtttaggc
    29401 ttctcgccat ttttagtgct ggctttaatt ttcacggtta atggttgatt gggacgcatt
    29461 tttgctggtg tttccagcgc cagatcgagg cgacggtttt catcaccaag cggcagatgc
    29521 aacacaccaa ccgcgcgttt tggcgtcgcg gagcgagatt tatcgccagg acgcaccacc
    29581 agcgtactca aataaagatc atgacgattc caggttttat cgaccggaat cgtcagatcc
    29641 agcccttgag ccggaacatc aatctcttgc caccacagcg gcccttcact ggactcgacc
    29701 atcgcataac ctttacccgc cgttggcgcg gcaatatgca acttaatggt atcgccgggg
    29761 cgataactgg ctttatctag tttcagcgtg acacggtcgg gtcgcactgc gccgctcccg
    29821 tcgctgttgt cctgccagct atagccagcc caaaaacgca cgctactgac cgcttcattc
    29881 ggcgctttga cttccagacg ataagcgccc cactctaccg ggaaactgac cttaccggtt
    29941 tcatccgcct gaagatccag agtttgttca ttttcgatca gatctttttg atcaaactgt
    30001 gactgccagc cttcatcttc tgaccagttc cagtagtaat cgcggcgttc gcgaatcagg
    30061 cgcacctgca agcccgacac ggcttttttc acgccttgcg catcgctata aacgatgtca
    30121 aaagcggcgt tactgccttc atcaacaatg ggctgtttca ccgtgctgtc agtgcgataa
    30181 tcgtaaaccg atttcaaggc gaactgcgga cggagccccg gcaatgcatc ggcaggccag
    30241 atagcctgct cagcgcggcg cgtcaccggg cgaccgcccg attccagcaa actgccctgg
    30301 aaaataacct gcaatgggga atgcgtttcc ttccactggc tttctgtaga aacttcgccg
    30361 cgccctttat catccagcgt caactgaact tcatccagcg tgcgggaaag gttttcggca
    30421 gctatatcgc cgaattcaaa accaggtaag gctgacacag cctcacgcag tgggcgcagg
    30481 aaaagttgcc cttgcaaagt attaccatta gcaggcgcac cgtacaggta gtatcccacc
    30541 acggagaatt tcacttcatc attcggcgta agcggggttt tctcaccggt cagattcagc
    30601 gccatacgct ctggcataaa atcttcgacg tggaaatccc acatccggta ctggttatcg
    30661 cccgtgttgg cgcgaatatg ccacataccg gttgccgcat tgctatcgag tggccaggta
    30721 aagtggtaga ggccattctc cggctgacta acgacgttcc tgagtacctg cccgtcaggt
    30781 ttaatcacgt ctaacttgat gggttgatcg ggcaatgctt taccgtctgc atcgcgcagt
    30841 aaaccattga ggattaccgt ttcacctggg cgataaagat cgcgtgggcc aaacatgaaa
    30901 aactgtttgc tatagcctgg cgcgccagca atgttaaatt ctgctaagtc cagcgccgga
    30961 agttttaaat cgagtagcgt tgtctgaccg tctttacgtg ccagcagtaa tgccgcattt
    31021 ttatcatttt ccagctgcac atgcccctga gcgtcactgg ttgcctgagt cagagtctgt
    31081 cctttctcat ttaataaaga gacttcaatt ccttgctggg ccgcgccgtt ttccagactt
    31141 tgggtaaaga tatccagacg attgtgataa cggtgagctg aaacgccgat atcacttaac
    31201 gtaaacagcg tcgcgggatt actgtaatcg taacgtccag cctgattcat cacagccaga
    31261 tacacgcccg cctgctgaag cggtttgata tcgcccagcg gcagcaataa tttttcacgg
    31321 gtgttacgcg caggattgag atcaaaccgt ccggtgtaga ccagatccgc catctgcagc
    31381 agtttgtctg actgccagtt cgccagcgaa ttgcggtatt cccattggct aatgaatgct
    31441 ggcagagatt ctggcttaac gcggaagaag ttaacatcga cattattaac gttgagcgcc
    31501 attaccggca gcccttcaac gactttgcca ggcagcagcg aaccacggct ggcaaaaccg
    31561 acgctgggtt ggatgtcgcg ggtagttata gttttttcgt aatctttact gaaggttgcg
    31621 ttgttgagtg ctttgacctc cttgccaata gtaacgatca aatcacgttt cggttcgagg
    31681 tggcgtaaac gtagctcttt aagattatct gacagctccc aggcaccatc cactttgccg
    31741 ctttttttat cgacgacatg aataacgcgt gagaaatcct gatccgggtc gagagggatg
    31801 gagaacgtca gcaccagcgt ggctgcacca tcaagttgga cttcagagag atccagcaat
    31861 gtcagcacct taccggcact ctgttgggcc agtttttgtc tctccggcac ggagagtttt
    31921 gctgaactcg cgttttctga agaggccgct ttagtaactt cagaaggcgc atcttttttc
    31981 accgctgttg gcgcgttatc gttgttgtcg caccctgcca gcgccagcat tagcatgcag
    32041 gcggctacgc gtaacttttt catttttcat ccctgccaca atggcccgtt agcaacgtcg
    32101 aataattatt atgcgtgaga atttacgtta tgtaattcag tatcacgccc atacaatccg
    32161 aattttagtg agaattgtgg ctcagacagc caaatcagcc tctttcgctt gtcgcttttc
    32221 actaaacatc cgacaattta gcctaacccc ggcaaaaatg gagatgccta tgtccacgac
    32281 atggtttgta ggagccgact ggctcgccga acatattgat gacccggaaa ttcagattat
    32341 cgatgcccgc atggcgtcgc ctggacagga agatcgtaac gttgctcagg agtatctgaa
    32401 tggacatatt cccggcgcag tgttttttga tatcgaagcg ctttctgatc acacttcccc
    32461 gcttccgcac atgctgccgc gcccggaaac gttcgccgtg gcgatgcgtg aattaggcgt
    32521 taaccaggat aagcacctga ttgtctatga cgaaggtaat cttttctcag ccccacgagc
    32581 atggtggatg ctgcgcacct ttggtgtaga gaaagtgtcg attctggggg gtggacttgc
    32641 aggctggcag cgcgatgatc tgctgttaga agaaggtgca gtagagctgc cggaaggaga
    32701 gtttaacgcc gcgtttaatc ctgaagccgt ggtgaaagta accgatgtat tattggcaag
    32761 ccatgaaaat acggcgcaaa ttattgatgc ccgcccggct gcacgtttta acgcagaagt
    32821 tgatgaacct cgcccaggtt tacgtcgcgg acatattccc ggagcactga atgttccgtg
    32881 gacggaactg gtgcgcgaag gcgaactaaa aacgaccgat gaactggatg cgatattttt
    32941 tggtcgcggc gtcagctacg acaaaccaat tatcgtcagc tgcggctctg gtgtaacggc
    33001 agccgtggtt ttgttagcac tcgcgacgct ggatgtgcca aacgtgaaac tgtacgacgg
    33061 cgcatggagt gaatggggcg cgcgggcaga tttaccggtt gagccagtga aataagtatt
    33121 ttacaggcaa taaaaaaccg ccgaatttgg cggtttttta ttgctggtct ggttcgcggc
    33181 ctttccagca ggttgtatta ccgtagtaat gcaagcgcgt ctcagcggag acaatactcg
    33241 ccagtaactc tctttttgtc aagcaaaaga gagttattat tgttctgtta gtgtattatc
    33301 cactgcggcc ctttccgccg tctcgcaagc gggcgctggc ttaaggaaag gatgttccgc
    33361 gaccgttaga gcaagcgcgt ctcagcgctt gctatcgcgg caatatcgcc agtggtgctg
    33421 tcgtgatgcg gtcttcgcat ggaccgcaca atgaagatac ggtgcttctg tatcgtactt
    33481 attgtttctg gtgcgctgtt ttcccaagta aataataacc ggagtctctc cggcgacaat
    33541 ttactggtgg ttaacaacct tcagagcagc aagtaagccc gaatgccgcc ctttagggcg
    33601 gcatatttta gattatccgg ttttgtttaa agtcacgcaa aaaaccaccc cagcgacgtt
    33661 catagaatgg cacaatatgt tcggtaataa agtggctaat tcctttttcc ccttttttca
    33721 cctgacaaat atcgattggt tcatcgccag gtaatgtatc ggtcgctaca cttcccgtcg
    33781 cctgaataat ttcttcgata tcaccatcgg cttcaatgcc aataagtaaa ttaggctgtg
    33841 cctcttcgtt ctctttaatt gaacaaataa aagcacgctt caccggctta atggttttaa
    33901 ataaggtggt gagtgaatca atcatttgtg ctggcggctc tgcgacttcc gataatatca
    33961 gcgattcacc gccttccagg atttcctggc tgctcagcgg atttccctct tcaccaatca
    34021 acaaactgat ttcacgcggc ataaattctt taccggtcgg cagtttggca ttaaggaaga
    34081 gcgtttcgcc aagggtcatc tcaaacaatg tgcgaacggg cattacgaca aatgcctgtt
    34141 cgtcttcaac cgcctgttga agcgcttcta acgaggtgaa aaaaggaatg acgctggtgc
    34201 cgtcttcttt ttcccagtgc tgtaaatcaa gcgcactatc ttcaaccaca gcctcgccct
    34261 gcgccgccgt accaggcacc cagacggtgg attccagtag agtacggaaa aaggccgggc
    34321 ggtgcgccgg ttcggttgct gctttttcca gcaggtcttc aagttcgttt tttgtttcgg
    34381 acataagaac cacaattcat tcaacgttcg gggcgcaaat gcctgatgcg ctacgcttat
    34441 caggcctaca agggattcgc aatttgttga atttgcagaa tttgtaggcc ggataaggcg
    34501 tttacgccgc atccggcata ttagtttacg ccgttaacag attagctatc gtgcgcacac
    34561 caagtcccgt agcgcccgca gaccactgtt caaccggcgc tttacggtaa gtcgccgagc
    34621 agtcgatatg cagccagcct tgctgatagt tctcaacaaa gtgcgacagg aagcccgccg
    34681 ccgtgctcgc gcctgccgga tacgccgcgc ttccggtatt gttcagttcg gcaaagttag
    34741 acggcagctg gctgcggtgg aattccgcca gcggcagacg ccagaatggt tcgttctctt
    34801 gtgaggcact cgccagcaaa cgaccggcaa gcgcatcgtc aaaactgaac agcgcgtgat
    34861 aatcattacc cagcgcagtt ttcgccgccc cggtgagggt cgccgcatca atgatcattt
    34921 ccggtttctg cgcactggca tcaatcagac catcggcaag caccagacgc ccttcggcat
    34981 cagtgttcat cacttcaact tttttaccgt tgcgataggt gatgatatcg cccagcttga
    35041 acgcattgcc gctaatcagg ttatccgcac agcagaggaa cagcttcacg cgcttgttca
    35101 gtccgcgcgt aatggcaaat gccagcgccc cggtaaccgt tgccgcgccg cccatgtccg
    35161 acttcatcga gtccataaac gcagtctgtt tgatgctgta gccgccggag tcaaaagtga
    35221 tacctttacc taccaggcac gcgtacactg gcgcttcttt atcgccagtt gggttgtaat
    35281 ccagcgccag caataccgga gaacgttctg aaccgcgtcc gacggtgtgc agccccatat
    35341 aaccttgctc acgcagatct tcgcctttgg tgatccgata agtcacacga tcgcccgcga
    35401 cgttgctgat cagatcaaca gcacgctgtg ccagttgcga tggtcccaat tcttctgccg
    35461 gtgcgttgat ggtgtcacgc acccagtcga tgatcatcag gcggttatcc agttcctggc
    35521 gctgggcatc gtccagatcc ggccactcca ctttacgcgt gcctttcggg gctttgtaac
    35581 cttgccagaa tgcccagcag cgatccgcat cccagccttc accgcttaac tgaacatgct
    35641 tgatgcccag accgtcaatc ttgcgcgccg cacgctggat cagccccaga tcgtctgccc
    35701 cgttcaaatg cagggtaatg ccgtcattat taatgctgta agttgctttt tctccccagc
    35761 gcgcgtcggc aggttgggta gagagggtaa tcttcatcgc ttctgtcatt ttagttatcc
    35821 ttctttgtaa aagggccgcc agttggcagc cccaggattt ttccgctaaa tgattcgcgt
    35881 tgcagaaagg cggcaagtga gtgaagcccc aggagcatag ataactatgt gactggggtg
    35941 aacgagcgca gccaacgcat ctgcggcgtg aagcatgacg cggaaattat tcggcctcgt
    36001 ccagccagac taacaaaatc gcttcgagga ttttctcgtt ggatgcctgc gggtcgtcgt
    36061 cgaaatcttc cagatcgcaa atccactgat gcatatcggt gaatcgaacc gttttcggat
    36121 caagatcagg atacgcatcg tacagtgctt cgccaatttc gcggctatcg gtccacttaa
    36181 gtcccatact aacctctgtt aatgctcacg cgcatggttg atagtgtaac gcgggatttc
    36241 gactactaaa tcttcgtcgg taacgcgtgc ctggcagctt aaacggcttt cgggctccag
    36301 tccccaggct ttgtccagca tgtcgtcttc ctgctctgag ctttctggca gcgagtcaaa
    36361 accttcacga acgatgcagt ggcaggtggt gcaagcacag gatttttcac aggcgtgttc
    36421 aatctcgata ccgttacgca gagctgcgtc gagaatggtt tcaccgctat tagcttccag
    36481 aacagcgcca tcaggacaga gatcctgatg aggcaaaata acaatctttg gcatattaaa
    36541 cctcgtccac ggaatggcct ttcagcgcac gacgaaccga ctggtccatg cggcgagcgg
    36601 cgaaatcctg ggtttgtttg tctacgtttt taatcgcttg ttcgatggcg tcaacgtcat
    36661 cgccctgcgc cacttcactc aggtgagcgg cagcatcgtc aatgacctga cgttctgcgg
    36721 cgcttaacag cgcggcatca gcagccagcg cgccgtgcag actttccagc acacgcgccg
    36781 cttctacttt ttgttctgcc agcattcggg cttttacgtc ctgctcggca tagctcattg
    36841 agtctttgat catcgaagcg atttcgctat cggtcagacc gtaagacggt ttgacctgaa
    36901 tagacgcctc aacgccggtg gatttctcca tcgccgtcac gctcaaaaga ccgtcggcat
    36961 cgacctggaa cgtcacgcga atatgcgcac caccagccgg tagcgccgga ataccacgca
    37021 gcgcaaaacg cgccagtgag cggcagtcct gcaccagttc gcgctcaccc tgcattacat
    37081 ggatagacat cgccgtctga ccatctttaa aggtggtgaa atcctgagcg cgggccaccg
    37141 gaatagtggt attacgcgga atcactttct ccaccaggcc gcccatcgtt tcgaggccca
    37201 gcgacagtgg gatcacatca agcagcagca tttcgctgtc tggcttgtta cccaccagaa
    37261 tatccgcctg aatcgccgcg ccaatagcga cgactttatc cgggtcgatg gaagtcagcg
    37321 gtggacgacc gaaaaattcg cctacccgtt cacgcaccag cggcacgcga gtagaaccgc
    37381 ccaccatcac cacttccagc acttcgtcag cttctacacc cgcgtcttta agcgcgcgac
    37441 gacaagccag taaggttcgt tttaccagtg gcgcgatcag ttcattgaat tgttcacggc
    37501 tgatttcgcc ctgccagccc gcaacgttaa cggtcacgga gtccgcatcg ctcagcgcga
    37561 ttttggctgc aatggcggca tccagcagtt cacgctgaac gcggttatcg ctacgatcag
    37621 gaatgcccgc ctgctcgcga atgtaatccg ccagcagatg gtcgaaatca tcgccgccga
    37681 gcgcggaatc accgccggtt gccagcactt caaacacgcc gcgacttaag cgcagaatgg
    37741 aaatatcaaa cgtcccgcca ccgaggtcat aaacagcgat cacgccttcc tgaccggaat
    37801 ccagcccgta ggcgatagcc gcagcggtcg gttcgttaag taagcgcaga acatgcaacc
    37861 ccgccagacg cgccgcgtct ttggtgccct gacgctgggc atcgtcaaag tacgccggaa
    37921 cggtgataac tacaccatcc agctcgcctg ccagggcttc agttgcccgc gccgccagtg
    37981 ctttgaggat gtccgcagaa acgcgcaccg ggttcagcag ccccgccgcc gtttcaatca
    38041 tcggcaggcc gttttcgctg gcctggaatt gataaggcag atgcggatag cgttgctgga
    38101 tatcagccag cgagcgcccc atcaggcgtt taacagaact aatggtgttg gcggtgtcaa
    38161 gcgctgcgtt agtacgcgcg tcataaccca ccgaatgccc ttgctgttga tagtgaacaa
    38221 cagatggcag caggtgacgg ccttcatggt cggctaacgt ttcggcctga ccgctgcgca
    38281 ctgtcgccac cagcgagttg gttgtgccca ggtcaatacc agccgccaga cgacgctgat
    38341 gcggcgcagc actcaaacca ggttcactaa tttgtaataa ggccatgttt agcttccaga
    38401 aattaaaaat cgagcagttt ttcttcgagt tgttcggcac tgctccgcag tttatcgaga
    38461 aaacgcagct tacgcacggt atccgccgcc gcgtcccacg cctcgttgtc taactgttca
    38521 accatcaact gatggcgggt atcaaacatc tttttcacac gtttgataaa gctttccagc
    38581 cgcgcttcat ctttcgcctg ttcgatctcg tccagctctt cgcgcagctc caactgttcc
    38641 atcaggaacg cggtgtcgcg cacagtatgc tgctcgctgg cgagatcaaa accgtgtaaa
    38701 gaaagcaaat attccgcgcg cattaacgga tgacgcagcg tttgccaggc ctggttaatg
    38761 gttgcagatt gctgtacggc ggcgagttgt tccgcctggc ttccgctggc gaatttatca
    38821 ggatgatact gacgttgtag atcctgaaaa cgcaggctca gcgcctgggt atcgagttga
    38881 taacgggcag gcaagccaaa gaaggtgaag taatccataa cattctcagg gctgcgatcc
    38941 gccgcgataa cggcgaatca ggtaaaacaa accccacgcg caggcgacca cggtggggtt
    39001 atcggtatgc gcatcaaacg tggaagcttt cgccgcaacc acactcatct ttgacgttcg
    39061 ggttggtgaa tttaaaccct tcgttcaggc cttcttttac gaagtccagc tgcgtaccgt
    39121 ccagaaattg caggcttttg ccatcgacca cgactttcac gcctttgtct tcaaacacga
    39181 tgtcttccgg cgtcggttcg tcaacaaatt ccagtacata agccatacct gaacatccgg
    39241 aggttctcac gcccagacgc aggccaaacc ctttgccgcg gttagccaga aaggtattta
    39301 ctcgcgctgc tgcactgtcg ctcagtgtaa tcgacataac caaacctcaa ctcttatttt
    39361 gcttcacgtt tgcttttata gtccgcaatg gcggctttga tcgcgtcttc tgccagaata
    39421 gaacagtgaa ttttcaccgg cggcagttca agttcttcag caatatcggt atttttgatc
    39481 gcctgcgctt cgtcgagaga cttccctttc acccattcgg tgaccaggga gctggaagcg
    39541 atagcagaac cgcagccgta agttttaaaa cgcgcgtctt caatgatacc ttcatcgttg
    39601 actttaatct gcaacttcat cacgtcgcca caggccggtg cccccaccat gccgctgccg
    39661 acgttctcgt cgttgttgtc aaaggaaccc acgttacgcg gattctcgta atggtcgata
    39721 actttttcgc tgtaagccat tataaattct cctgattccg ataccgatta atgatgagcc
    39781 cattcgatgc tgttcagatc cacgccctgc ttgtacattt cccacagcgg agaaaggtca
    39841 cgcagacgac cgatggattt acgaactaac tcgatggtgt agtcgatctc ttcttcagta
    39901 gtaaaacgac ctaaagagaa acggatagag ctatgtgcca gctcgtcgtt cagccccagc
    39961 gcgcgcagca cgtaggacgg ttcgaggctt gctgacgtac aggcggaacc tgaagaaact
    40021 gcgaggtctt tcagcgccat aatcagcgac tcaccttcaa cgtagttgaa gctgacgttg
    40081 agaatgttcg gcgcaccgtg ttccaggtca ccgttcaggt aaacttcttc gatatctttg
    40141 atgccgttcc acagacggtt acgcaggccg cgcagacgtt ccatctcggt cgccatctct
    40201 tcttttgcga tgcgataggc ctcgcccatt ccgacgatct ggtgaacagg cagagtgccg
    40261 gaacgcatac cgcgctcgtg accgccgccg tgcatttgcg cttcgatgcg tacgcgcggt
    40321 ttacgacgta catacagcgc accgatacct ttcgggccat agattttgtg accggagaaa
    40381 gacatcaggt caactttcaa ctggctcagg tcgataggca gtttgcccac gctctgggtt
    40441 gcatcaacgt gatagataat gccacgagca cggcacattt cgccgatagc cgcgatatcc
    40501 tgcaccacgc cgatttcgtt atttacgtgc atgatggaaa caaggatggt gtcatcacgc
    40561 atcgctgctt caagttcttt cagatcgata atgccgttac gctgcggtgc caggtaggtg
    40621 acttcaaaac cttcgcgctc cagctgacgg caggtatcca gtaccgcttt gtgttcggtt
    40681 ttgctggtga tgatgtgctt gccttttttc tgataaaagt tggctgcacc tttgatcgcc
    40741 aggttgtcag attcggttgc accagaggta aagacgattt cacgcggatc agcgccgacc
    40801 agatcggcaa tctgattacg ggcgatatct accgcttctt cagcctgcca gccgaaacgg
    40861 tgagaacggg aggccgggtt accaaaggtt ccgtccatcg tcataaactg catcattttc
    40921 tcggcaacac gcgggtccac cggcgtggtt gcggagtagt cgagataaat cggtaatttc
    40981 attgctctat aaactccgta catcactcaa tgcaaggaac caggctaccg gctggatgta
    41041 cgaccgtgtt tacggagcgc gatacgcccc ggcctgattc taatatcttg ttatctttaa
    41101 cctaactgtt tcgggtttca gcaagttgac aaacttacga atctccggga gcttacataa
    41161 gtaagtgacc ggagtgagta tgtgcagtca acgcagatgc aacacgaaac aggacggtta
    41221 aattaagcgc gtaacttaac gtcgatcgcg tcttgtatgc gggtgcgtgg cgcgtcgtga
    41281 gtatgctgac gaccagacac atccagcact tcctggttat taaccagttc gcctaaagta
    41341 atgttgttga gaaaaccggt gagacggtcg ctcaaatcac gccacagcgc gtgggtcagg
    41401 catttatcgc cgccctggca accgccttta ccctgacaac gggtagcatc tacagattcg
    41461 tcaacggcgc taatcacttc gccaacggcg atgctgctgg catctttgcc taacagataa
    41521 ccaccgcctg gtccacgtac gctggaaacc agaccatttt tacgcagacg ggaaaacagt
    41581 tgttccagat aagaaaggga aattccctga cgttcggaaa tatcagccaa cggtaccggg
    41641 cccgcttcag agttgagcgc aacgtcaagc attgcggtca cggcatagcg ccctttagat
    41701 gtcagtctca tgtcttactt cacctcaaac tcgcccctgc ccggggtttt ttattgtaaa
    41761 agtgggggta ttgcatagca gggtcaagtc tgacattccc gagtaaattg gtcaactatt
    41821 tacttgactg atttagtcgg gtatttaacc ttcagtgcca tttttttatc gtggcgtttg
    41881 tacctgttgt ccttccagac catgggcggg ttgagggcaa ttattcgcat tagcgctggc
    41941 ctcttcccct caccctaacc ctctccccga aggggcgagg ggaccgtccg tgcatttatc
    42001 tggcttagcc ctcaggcctt attaccttta ttctgctgct ctatagaagc cagaatcccg
    42061 cgcaggatat tcaattcctg gctttccggg cgcgcacggg taaacagacg gcgcaattta
    42121 ttcatcacct gccccggatg gttttcacgg ataaaaccgg ttgccagcag cgtttgttcc
    42181 agatgaccat aaaaacgctc cagatcatcg accagcggat acggcgtctc ttcatgctcg
    42241 acctgctcgc cattttcctg agttgccagc caggccatac gcacttcata ggcaataacc
    42301 tgaaccgcca ttgccaagtt cagcgaactg tattccgggt ttgccgcaat cgcgacatga
    42361 taatggcatt tctgcaactc ttcattggtc aagccgacgc gctcacgacc aaacaccagc
    42421 gccaccgggg tatttgccgc ttcagcgacg cttttcagac cgcattcgcg cgggtcgagc
    42481 atcggccacg gcagcgtgcg ggagcgtgcg ctggtgccca ccaccaggct acaaccggct
    42541 aacgcttcgt cgagcgtatc gacgatgtga gcattaccaa tcacatcgct ggcccctgct
    42601 gccagggcaa tcgcctggga gtcgggtttc accagtggat taaccagcca cagattggtt
    42661 aatcccattg ttttcatggc acgggcaaca gaacccatat tgccggtgtg tgacgtctcc
    42721 accagcacaa ttcgaatatt ttgcagcatt gtctttcttc atctaaagat tattcacgca
    42781 tcttatcata aaacgaagac agatgccgat ctcgctgcta tactctgcgc cgttttcccg
    42841 ttctttaaca tccagtgaga gagaccgatg catccgatgc tgaacatcgc cgtgcgcgca
    42901 gcgcgcaagg cgggtaattt aattgccaaa aactatgaaa ccccggacgc tgtagaagcg
    42961 agccagaaag gcagtaacga tttcgtgacc aacgtagata aagctgccga agcggtgatt
    43021 atcgacacga ttcgtaaatc ttactcacag cacaccatca tcaccgaaga aagcggtgaa
    43081 cttgaaggta ctgatcagga tgttcaatgg gttatcgatc cactggatgg cactaccaac
    43141 tttatcaaac gtctgccgca cttcgcggta tctatcgccg ttcgtatcaa aggccgcacc
    43201 gaagttgctg tggtatacga tcctatgcgt aacgaactgt tcaccgccac tcgcggtcag
    43261 ggcgcacagc tgaacggcta ccgtctgcgc ggtagcaccg ctcgcgatct cgacggtacc
    43321 attctggcga ccggcttccc gttcaaagca aaacagtacg ccactaccta catcaacatc
    43381 gtcggcaaac tgttcaacga atgtgcagac ttccgtcgta ccggttctgc ggcgctggat
    43441 ctggcttacg tcgctgcggg tcgtgttgac ggtttctttg aaatcggtct gcgtccgtgg
    43501 gatttcgcgg caggcgagct gctggttcgt gaagcaggcg gcatcgtcag cgacttcacc
    43561 ggtggtcata actacatgct gaccggtaac atcgttgctg gtaacccgcg cgttgtgaaa
    43621 gccatgctgg cgaacatgcg tgacgagtta agcgacgctc tgaagcgtta atgactcagg
    43681 cgggtgatgt cactcacccg ccctcgcctt tcaggcgcta ttctgaaata cttcctcacc
    43741 gctttacttt ctttcgtcac tctcccacca tctttccccg attaatggat gataaagaag
    43801 tcacaaggaa aggttatggc gctgccagtg aacaaacgcg ttctcaaaat tctgtttatt
    43861 ctctttgttg ttgccttctg cgtttattta gtgccgcgcg ttgccatcaa cttcttctat
    43921 tatcctgacg ataaaattta cggtcccgac ccctggtcgg cggaatccgt cgaatttacg
    43981 gctaaggacg gtactcgtct gcaaggctgg tttattcctt cttcgacggg ccctgctgac
    44041 aatgccatcg caaccatcat tcatgctcac ggcaatgccg gaaatatgtc cgcccactgg
    44101 ccgctggtca gttggttacc cgagcgtaat ttcaacgttt ttatgtttga ttatcgcggg
    44161 tttggtaaat caaaaggcac gccgtcccag gccggattgc tggacgatac gcaaagtgcc
    44221 atcaatgtgg tgcgccatcg cagtgatgta aacccacaac ggctggtgct gttcgggcag
    44281 agtattggcg gggcgaatat tcttgctgtt attggtcagg atgatcgtga aggcatacgt
    44341 gcggtgatcc tcgactccac atttgcctct tatgcaacca tcgccaatca gatgatcccc
    44401 ggcagtggct acttacttga tgagagttac agcggcgaaa attatatcgc cagcgtcagc
    44461 ccgatcccgc ttttactcat tcacggtaaa gctgatcacg ttatcccatg gcagcacagc
    44521 gaaaagttgt atagcctggc aaaagagaca aaacggttga tcctaatccc ggatggcgaa
    44581 cacattgatg ctttttccga tcgtcacggc gatgtttatc gcgaacagat ggtggacttt
    44641 atccttagcg cgttgaatcc gcagaactaa cccatgatcg ctagcacgat aatcattcac
    44701 aaaaccacct taagacatgc taatccactg gtcagaacag tttaagatga gaaaaattct
    44761 gtgacgcttg ccaacatttc tgatgattag cattcccttc gccatctccc tgagcaaact
    44821 ttagctattc ttatcaatta tgcttatggg agatctacag atgatgccta cgcttgctcc
    44881 accatctgtc ctttcggctc cccagcgccg ctgccagatc ttgctgacgc tctttcagcc
    44941 ggggttgacc gccaccatgg caaccttcag cgagcttaat ggtgtggatg atgatattgc
    45001 cagtcttgat atcagcgaaa caggacggga gatcctgcgc tatcatcaac tcacactgac
    45061 gactggttat gacggtagct accgggttga aggtacagtg cttaaccaac gtttgtgttt
    45121 atttcactgg ctacgacgtg gtttccgtct gtgtccgtca tttattacca gccaattcac
    45181 ccccgccctg aagagtgaac tgaagcggcg cggaattgcg cgtaactttt acgacgatac
    45241 caatctacaa gcgttagtga atctctgctc ccgacggctg caaaaacgct ttgaaacgcg
    45301 cgatattcat ttcctgtgtc tgtatctgca atattgtttg ctgcaacacc acgctggaat
    45361 tacgcctcag tttaatccgc tccaacgtcg ctgggcggaa tcctgccttg aatttcaggt
    45421 agcgcaggaa attggacgcc actggcagcg tcgggcgctc cagcctgtac cgcctgatga
    45481 gccactgttt atggcattac ttttttccat gttgcgggtt cccgatccat tgcgtgatgc
    45541 gcatcagcga gacagacaat tgcgtcagtc tatcaaacgt ctggtaaacc attttcgtga
    45601 gctgggaaat gttcgttttt atgatgaaca ggggttatgc gatcagcttt atacccacct
    45661 cgcccaggcg ttaaatcgca gtttgtttgc catcggtatt gataataccc tgccggaaga
    45721 gttcgccaga ctgtacccac gcctggtgcg caccacccgc gcggcgctgg ccggatttga
    45781 aagtgaatac ggcgtccatc tttctgatga ggaaagcggt ctggtcgcgg tgattttcgg
    45841 tgcctggcta atgcaggaaa acgatctgca tgaaaaacag attattctac tgaccgggaa
    45901 tgatagcgag cgagaagcgc agattgagca gcagttacgc gaactaacgt tactgccgct
    45961 caacattaag catatgtcgg taaaggcatt tttgcagaca ggcgctccgc gtggcgcggc
    46021 actgattatt gcgccttata ccatgccatt accgctcttt tcaccaccgc tgatctatac
    46081 ggacctgacg ctgacaacac atcaacagga gcagatccgc aaaatgctcg aatcagcatg
    46141 agggaacaac tttcgggcgc aaaaacattg ccggaagcgc caccagcgcc attacccaga
    46201 acacgccgtg gcccagatat tgatacagga aaccggcgaa aacggtcatg atagcgatac
    46261 tgccgcccat cgcgacggca gagtaaaccg cctgtaaacg gatgacttcg ctaccctggc
    46321 gaacagcaat atagcgcatg gcggccaggt ggcagaccgt gaaggtgccg caatgcaaaa
    46381 tttgcaccac tatcaaccac ggcaacgccg tagttgctcc cataatgccc cagcgcacta
    46441 cgccgcaaat cgccgagatc aacagcatat cgcgagcact acaacggcgg aaaagtttat
    46501 tactcagcgc aaagataatg acttccgcca ccacgcccag cgaccacaaa taccccaccg
    46561 ccgaggccga gtagccagct gcctgccagt aaatggcgct aaaaccgtaa taggccgaat
    46621 gtgccccctg caataaacaa acgcaggcca gaaagcgcca gttctggcga accagcgcca
    46681 accacgcaga ccaaccggtg ctctcctgct ggcgacttgc cccttgtggc tgaatcgtcg
    46741 gacggatgag aaagccgagc agcatggatg ccacgcccaa cgtcaacagc gcgaggatca
    46801 cccgataatc aaacatagtg accagtttgc ccgtcagcgc cgagccaatg acaaacgcca
    46861 ccgagcccca cagtcgcact ttgccgtaat caagcgggaa ctgcttttgc cacgtattcg
    46921 ccagtgcatc ggtcaacggt accagcggtg agaaaaagag gttaaagcca atcatcacca
    46981 gcatcagcca cgctacgtgc gcccccgccc agaaggcgac agcaaagaga agtgtcagca
    47041 gtgccagcac gcgcaaggcg gaaatcaggc gggaaggatc gctgacgcgg ggcgcgatga
    47101 gcaaactccc gagaaaacgg gcaaccagac ctgcccccaa taacaggccg atggtttctg
    47161 gcgttaaacc aatcccttta agccagacgc tccagaaagg tagaaaaatg ccgtaactaa
    47221 aaaagtatgt gaaatagccg agcgccaacc agcgcgtgga ttgcaaaacc atgagtccct
    47281 cccgtcagga ggcgttagtc tggggttaat cgaatgttgg tgcaagttga aaaataagaa
    47341 tattcgagcc tgcttcccga tgtctgtaac gccagttcac aagcactaaa aaactgcgat
    47401 ccgcgacgca tgttgcaaaa ataatgcgta attatgccgt tacgcttgcc aaacgttcct
    47461 gcacaatggc gatgaaatcg cgtaatgccg gcttcatctc ccctttcttc cacgccatca
    47521 gtaaagcaat ggaaggcaca tttccggcaa tgggacgaaa aacaacctgc ccggtattaa
    47581 aattattcat ataaccgggt atcaaagtga cgcccagccc catgcccacc agattcatgg
    47641 tcaccagaat attcgttgcc acctggacga tatttggctg gctgttttct tgcgcaaacc
    47701 acgctttaac gatcggcgca agcgaaccgg aatacgccgg atcggtactg acgaaattca
    47761 cgccatccag ttgcgcggcg gtgatctctt tttcatgtgc taaagggtga tcaaccggta
    47821 acaccaccac taatggttcg tcaaaaagct ccagataatc aatctccggg ctataaacag
    47881 gatggcgcat taagccgacg tcgagttcac cacggcgaat tttttcctcc tgttgcgtgg
    47941 tgattaaact caccagctca atcaaggtgt ctggctgtct gagacgaaac attggtaata
    48001 cttttggcaa taaattcact tccgccgatg gcacaaagcc aatggttaat tgtctgtctt
    48061 cctgaacaat tttccgcgcc cgtaatttgg cattttccgc ttgttcgagg attgccagcg
    48121 cgtcctggag aaaacattct cccgccgccg tcaacgcgac tttgcgctta tccctcacca
    48181 gtaacggaac accgacacag ttttcaagat cgcggatctg gctgcttaac gaaggctgtg
    48241 aggtatgcag tttttccgcc gcacgggtaa agttcagtgc ctgcgccact gcgacgaaat
    48301 agcgtaaatg ccgtagttcc atcaccttcc ccttgttatc gaaaaaacgt ctcagctggt
    48361 agaaattaac tatttcacat attagcaacc aaccagcaac atccttatga cacaaaaata
    48421 gaaggtcaat acatcttatc tttcaggatt aaaaaatatg accacaccct cagatttaaa
    48481 catttaccaa ctgattgata cccaaaatgg tcgggtcact ccgcgtattt ataccgaccc
    48541 agatatttac caactggagc ttgaacgtat tttcggccgt tgctggttat ttctcgccca
    48601 cgaaagccag atcccaaaac ctggtgattt ctttaacacc tacatggggg aagatgcggt
    48661 tgtcgtagtg cgtcagaaag acggcagcat taaggcgttt ctcaaccaat gccgccaccg
    48721 ggccatgcgt gtgagttatg cagattgcgg caacactcgc gcctttacct gtccgtatca
    48781 cggctggtct tatggcatta acggcgagtt gatcgatgta ccgctggaac ctcgcgccta
    48841 cccacaaggg ttgtgtaaat cccactgggg actaaacgaa gttccttgtg tggagagtta
    48901 taaagggctg atttttggca actgggatac cagcgcaccg ggcctgcgtg attacctggg
    48961 tgacattgcc tggtatctgg atggcatgct ggatcgtcgc gaaggcggca ccgaaattgt
    49021 cggcggcgta caaaagtggg tgatcaactg taactggaaa ttcccggcag agcagttcgc
    49081 cagtgaccag tatcatgctc tgttcagcca tgcttctgcc gttcaggtat taggggcgaa
    49141 agatgatggc agcgataagc gcctcggtga tggacaaacc gcccgcccgg tgtgggaaac
    49201 cgccaaagat gcgctgcaat ttggtcagga cggtcacggt agcggtttct tctttactga
    49261 aaaaccggat gctaatgtct gggtcgatgg cgcagtttca agctattacc gcgaaaccta
    49321 tgccgaagca gaacaacgtt taggtgaagt tcgcgccctg cgcctggcgg gtcataacaa
    49381 tattttcccc acgctttcat ggctcaacgg cactgccacg ctccgcgtct ggcatccgcg
    49441 cggccctgat caagttgaag tgtgggcgtt ctgtattact gacaaagccg cctccgatga
    49501 agttaaagcc gcttttgaaa acagcgccac tcgtgctttt ggtcctgctg gttttctcga
    49561 gcaggatgac tcggagaact ggtgtgaaat ccagaaattg cttaaaggcc accgcgcccg
    49621 caacagcaaa ctgtgtctag aaatggggct tggtcaggaa aagcgccgcg acgacggcat
    49681 tcctggcatt actaactata tcttttcaga aactgccgct cgtggaatgt accaacgctg
    49741 ggccgatctt ctgagtagcg aaagctggca ggaagtgctc gataaaaccg ccgcttacca
    49801 gcaggaggtg atgaaatgag tgcgcaagtt tcactagagt tacatcaccg cattagccag
    49861 tttctctttc acgaagccag cttactggac gactggaaat ttcgtgactg gctggagcag
    49921 ctcgacaaag agattcgtta caccatgcgc accacagtta acgcgcaaac acgcgaccgc
    49981 cgcaaaggcg tccagccacc gacaacctgg atttttaatg acaccaaaga ccagctggag
    50041 cggcgaatcg cccgtctgga aacgggcatg gcctgggcag aagagccgcc gtcacgcacc
    50101 cgtcacttaa tcagcaactg ccagataagc gaaaccgaca tcccaaacgt atttgctgtg
    50161 cgggtaaatt atctgcttta tcgggcacaa aaagagcgcg atgaaacatt ctatgttgga
    50221 acgcgtttcg acaaagttcg ccgtctggaa gatgacaact ggcgcttgct ggaacgggat
    50281 atcgtcctgg atcaagcggt aatcacttcc cataacctga gtgtactgtt ctgatgaatc
    50341 gaatttatgc gtgtcccgtt gcggatgtgc cggagggtga ggctctccgg atcgatacct
    50401 cgcccgtcat cgccctgttc aacgttggcg gcgagtttta tgccattaac gatcgttgca
    50461 gccatggtaa tgcgtcgatg tcagaagggt atctggaaga tgacgccacg gtggagtgcc
    50521 cgctacacgc cgccagtttt tgcctgaaaa cggggaaagc gttatgcctg cccgccaccg
    50581 atccgctcac cacttatcca gtacacgttg aaggtggtga cattttcatc gacttaccgg
    50641 aggcgcagcc atgagcgatc tgcataacga gtccattttt attaccggcg gcggatcggg
    50701 attagggctg gcgctggtcg agcgatttat cgaagaaggc gcgcaagttg ccacgctgga
    50761 actgtcggcg gcaaaagtcg ccagtctgcg tcagcgattt ggcgaacata ttctggcggt
    50821 ggaaggtaac gtgacctgtt atgccgatta tcaacgcgcg gtcgatcaga tcctgactcg
    50881 ttccggcaag ctggattgtt ttatcggcaa tgcaggcatc tgggatcaca atgcctcact
    50941 ggttaatact cccgcagaga cgctcgaaac cggcttccac gagctgttta acgtcaacgt
    51001 actcggttac ctgctgggcg caaaagcctg cgctccggcg ttaatcgcca gtgaaggcag
    51061 catgattttc acactgtcaa atgccgccag gtatcctggc ggcggtggcc cgctgtacac
    51121 cgccagtaaa catgccgcaa ccggacttat tcgccaactg gcttatgaac tggcaccgaa
    51181 agtgcgggtg aatggcgtcg gcccgtgtgg tatggccagc gacctgcgcg gcccacaggc
    51241 gctcgggcaa agtgaaacct cgataatgca gtctctgacg ccggagaaaa ttgccgccat
    51301 tttaccgctg caatttttcc cgcaaccggc ggattttacg gggccgtatg tgatgttggc
    51361 atcgcggcgc aataatcgcg cattaagcgg tgtgatgatc aacgctgatg cgggtttagc
    51421 gattcgcggc attcgccacg tagcggctgg gctggatctt taaggaagca cgatgaaaga
    51481 aaaaacgatc attattgtcg gtggcgggca agcggcggca atggctgcgg cctcgctacg
    51541 ccagcaaggg ttcaccggtg agctgcatct gttttccgat gagcgacatc ttccttatga
    51601 acgacctcca ctctcgaaat ccatgttgct ggaagattcc ccgcagttac agcaggtgtt
    51661 acccgctaac tggtggcagg aaaacaatgt tcatctgcat tccggtgtaa ccatcaaaac
    51721 gctgggtcgc gacacacgag agttagtgtt aaccaacggc gaaagctggc actgggatca
    51781 gctttttata gcaatcggcg cggcagctcg accgctgccg ttgcttgatg cactgggaga
    51841 acgctgcttt accctacgcc atgccggtga tgccgccaga ctgcgagaag ttctgcagcc
    51901 cgaacggtca gtcgtgattg tcggtgccgg aactattggt ctggaactgg ctgccagcgc
    51961 cacgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca tgggccgtaa
    52021 tgcaccaccg cccgtgcaac gctatctttt acagcgccac cagcaggctg gtgtgcgcat
    52081 tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac tgacgctgca
    52141 aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca gcgccaacga
    52201 gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg atgaggcttg
    52261 ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc gtcttgataa
    52321 tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc aaattgccgc
    52381 tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct ggagcgatca
    52441 gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc tttgtcgtgg
    52501 caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc ttatcggtgc
    52561 ggtcacgctg aatcaggggc gtgagattcg cccaattcgc aaatggatcc agagcggcaa
    52621 aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac tgtaaccagg
    52681 ataattagcg aatatctcaa cgcctggggc gtggcgaggt gcaagagtgt gtattacgtt
    52741 taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgt
//
LOCUS       DAZPBM010000008        50302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPBM010000008 DAZPBM010000000
VERSION     DAZPBM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.008"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="ACHUZQ_000401"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="ACHUZQ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1024198.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHUZQ_000402"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHUZQ_000402"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1024199.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHUZQ_000403"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHUZQ_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1024200.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="ACHUZQ_000404"
     CDS             4216..4620
                     /locus_tag="ACHUZQ_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1024201.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="ACHUZQ_000405"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="ACHUZQ_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1024202.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="ACHUZQ_000406"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="ACHUZQ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1024203.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHUZQ_000407"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHUZQ_000407"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1024204.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHUZQ_000408"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHUZQ_000408"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1024205.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9472
                     /locus_tag="ACHUZQ_000409"
                     /pseudo
     CDS             8043..9472
                     /locus_tag="ACHUZQ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9542..10318
                     /gene="yaaA"
                     /locus_tag="ACHUZQ_000410"
     CDS             9542..10318
                     /gene="yaaA"
                     /locus_tag="ACHUZQ_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1024206.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10471..10767)
                     /locus_tag="ACHUZQ_000411"
     CDS             complement(10471..10767)
                     /locus_tag="ACHUZQ_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1024207.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="ACHUZQ_000412"
     CDS             complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="ACHUZQ_000412"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1024208.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="ACHUZQ_000413"
     CDS             complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="ACHUZQ_000413"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1024209.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="ACHUZQ_000414"
     CDS             complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="ACHUZQ_000414"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1024210.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15691..15810)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="ACHUZQ_000415"
     CDS             complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="ACHUZQ_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1024211.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16025..16711)
                     /locus_tag="ACHUZQ_000416"
     CDS             complement(16025..16711)
                     /locus_tag="ACHUZQ_000416"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1024212.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17111..17251)
                     /gene="yjjY"
                     /locus_tag="ACHUZQ_000417"
     CDS             complement(17111..17251)
                     /gene="yjjY"
                     /locus_tag="ACHUZQ_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1024213.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17347..18063
                     /gene="arcA"
                     /locus_tag="ACHUZQ_000418"
     CDS             17347..18063
                     /gene="arcA"
                     /locus_tag="ACHUZQ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1024214.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18123..19475)
                     /gene="creD"
                     /locus_tag="ACHUZQ_000419"
     CDS             complement(18123..19475)
                     /gene="creD"
                     /locus_tag="ACHUZQ_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1024215.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19533..20957)
                     /gene="creC"
                     /locus_tag="ACHUZQ_000420"
     CDS             complement(19533..20957)
                     /gene="creC"
                     /locus_tag="ACHUZQ_000420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1024216.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20957..21646)
                     /gene="creB"
                     /locus_tag="ACHUZQ_000421"
     CDS             complement(20957..21646)
                     /gene="creB"
                     /locus_tag="ACHUZQ_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1024217.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21659..22132)
                     /gene="creA"
                     /locus_tag="ACHUZQ_000422"
     CDS             complement(21659..22132)
                     /gene="creA"
                     /locus_tag="ACHUZQ_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1024218.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22343..23212
                     /gene="robA"
                     /locus_tag="ACHUZQ_000423"
     CDS             22343..23212
                     /gene="robA"
                     /locus_tag="ACHUZQ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1024219.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="ACHUZQ_000424"
     CDS             complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="ACHUZQ_000424"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1024220.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23908..24429
                     /gene="yjjX"
                     /locus_tag="ACHUZQ_000425"
     CDS             23908..24429
                     /gene="yjjX"
                     /locus_tag="ACHUZQ_000425"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR1024221.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="ACHUZQ_000426"
     CDS             complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="ACHUZQ_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1024222.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="ACHUZQ_000427"
     CDS             complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="ACHUZQ_000427"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1024223.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27078..28745
                     /gene="ettA"
                     /locus_tag="ACHUZQ_000428"
     CDS             27078..28745
                     /gene="ettA"
                     /locus_tag="ACHUZQ_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1024224.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28906..29013)
                     /gene="nadS"
                     /locus_tag="ACHUZQ_000429"
                     /pseudo
     CDS             complement(28906..29013)
                     /gene="nadS"
                     /locus_tag="ACHUZQ_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="ACHUZQ_000430"
     CDS             complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="ACHUZQ_000430"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1024225.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30305..31687)
                     /gene="radA"
                     /locus_tag="ACHUZQ_000431"
     CDS             complement(30305..31687)
                     /gene="radA"
                     /locus_tag="ACHUZQ_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1024226.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31736..32704)
                     /gene="serB"
                     /locus_tag="ACHUZQ_000432"
     CDS             complement(31736..32704)
                     /gene="serB"
                     /locus_tag="ACHUZQ_000432"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1024227.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32810..33454
                     /locus_tag="ACHUZQ_000433"
     CDS             32810..33454
                     /locus_tag="ACHUZQ_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1024228.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33482..34498
                     /gene="lplA"
                     /locus_tag="ACHUZQ_000434"
     CDS             33482..34498
                     /gene="lplA"
                     /locus_tag="ACHUZQ_000434"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR1024229.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34530..34793
                     /locus_tag="ACHUZQ_000435"
                     /pseudo
     CDS             34530..34793
                     /locus_tag="ACHUZQ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="ACHUZQ_000436"
     CDS             complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="ACHUZQ_000436"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1024230.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="ACHUZQ_000437"
     CDS             complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="ACHUZQ_000437"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1024231.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="ACHUZQ_000438"
     CDS             complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="ACHUZQ_000438"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1024232.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="ACHUZQ_000439"
     CDS             complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="ACHUZQ_000439"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1024233.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39514..41064
                     /locus_tag="ACHUZQ_000440"
     CDS             39514..41064
                     /locus_tag="ACHUZQ_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1024234.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41036..41899
                     /locus_tag="ACHUZQ_000441"
     CDS             41036..41899
                     /locus_tag="ACHUZQ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1024235.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(42415..43197)
                     /locus_tag="ACHUZQ_000442"
     CDS             complement(42415..43197)
                     /locus_tag="ACHUZQ_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1024236.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(43194..44267)
                     /locus_tag="ACHUZQ_000443"
     CDS             complement(43194..44267)
                     /locus_tag="ACHUZQ_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1024237.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(44389..44550)
                     /locus_tag="ACHUZQ_000444"
     CDS             complement(44389..44550)
                     /locus_tag="ACHUZQ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1024238.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44677..45282)
                     /gene="osmY"
                     /locus_tag="ACHUZQ_000445"
     CDS             complement(44677..45282)
                     /gene="osmY"
                     /locus_tag="ACHUZQ_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1024239.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45675..47264)
                     /gene="prfC"
                     /locus_tag="ACHUZQ_000446"
     CDS             complement(45675..47264)
                     /gene="prfC"
                     /locus_tag="ACHUZQ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1024240.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(47355..48032)
                     /gene="yjjG"
                     /locus_tag="ACHUZQ_000447"
     CDS             complement(47355..48032)
                     /gene="yjjG"
                     /locus_tag="ACHUZQ_000447"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1024241.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(48047..48493)
                     /gene="rimI"
                     /locus_tag="ACHUZQ_000448"
     CDS             complement(48047..48493)
                     /gene="rimI"
                     /locus_tag="ACHUZQ_000448"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1024242.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(48462..48875)
                     /gene="holD"
                     /locus_tag="ACHUZQ_000449"
     CDS             complement(48462..48875)
                     /gene="holD"
                     /locus_tag="ACHUZQ_000449"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1024243.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            48978..50009
                     /gene="rsmC"
                     /locus_tag="ACHUZQ_000450"
     CDS             48978..50009
                     /gene="rsmC"
                     /locus_tag="ACHUZQ_000450"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1024244.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        11838 a        13627 c        12709 g        12128 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagcg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcgatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 atattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt
     8341 ttatcggcat ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg
     8401 acgtcaatgg acagtttcgt ggaggaccgg catggtatat ggcgcgcggg ctggggatgc
     8461 gttggatggg cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg
     8521 gagttcaggc gaacgccgtt gcccgagccc tgagttttct tttgattttc ccccgctggt
     8581 gacaggcatt attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt
     8641 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtgc tgaccagcat
     8701 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
     8761 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
     8821 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa
     8881 cgcggcagcg gcagcggcgt cctggcctcc gcatccggca gcgcaaggga ttgtccagat
     8941 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
     9001 ggcgggtaac gacacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
     9061 gcgggtgcta atgggttcct ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc
     9121 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
     9181 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
     9241 caccttgcta agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
     9301 tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga
     9361 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
     9421 tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
     9481 atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga
     9541 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
     9601 gcgctatacg ctgccggagc tgttagacaa ttcccagcac ttgatccatg aagcgcggaa
     9661 actgacgcct ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa
     9721 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggctat
     9781 tttggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaactttca gcgaagacga
     9841 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc
     9901 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg
     9961 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
    10021 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
    10081 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
    10141 atttaagata atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
    10201 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    10261 tgatgaagct tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    10321 ttgcagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt
    10381 caatatattg cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg
    10441 aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    10501 gccgtgatga tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg
    10561 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    10621 acctccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg
    10681 gactaacgta atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc
    10741 gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    10801 cgatatccgg agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct
    10861 aagttaccgc atttattgag aatttctcct ttttccctgt cgtttttctc cataatttct
    10921 tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt
    10981 ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga
    11041 aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    11101 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    11161 ttcgcctgga ttcaactggt cacacagcgc acgatacgct acggcagcgt gcggctccga
    11221 ggtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    11281 tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    11341 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    11401 tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    11461 aaaacgtttc accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa
    11521 gtttccgctt ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac
    11581 tgcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagccga
    11641 gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg
    11701 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca acgtacagaa
    11761 cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt
    11821 cggcaaaccg tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag
    11881 aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    11941 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    12001 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    12061 ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    12121 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcat gcggaaaaaa
    12181 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcgtt
    12241 gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    12301 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    12361 ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg aacaaggtcg ggccggagcc
    12421 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    12481 aggcagtaac cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag
    12541 ctcaggctga cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat
    12601 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    12661 aattcccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    12721 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    12781 gtaatgaatg ctgccggaga tacgtccttc cagctcgccc atcaaagcca gcaaacgggt
    12841 gtcattaagt ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccaccgaaca
    12901 ggcgctggag cctaagcccg agccgatcgg catattcttt tccagagtca tcgccactgg
    12961 aatttgcttg cccagctcca ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    13021 tggttctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc
    13081 cgcctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc
    13141 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    13201 gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca ccagcagcgg
    13261 taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc
    13321 tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttcccatcca
    13381 cttcggcaat cttcacgcgg cagacaccat cttcatcaat attgccaaca tagcgcaaaa
    13441 cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    13501 gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc agcacaggtt
    13561 caatttcaat atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagtt
    13621 tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta taacccattt
    13681 cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    13741 aagaaagcga acctgaaaga atgccggaga acttcaccaa ttcatcacct gcattgagca
    13801 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    13861 tacgccgcga tttttccgcc gcataacgca actgatgata gtaatccatc gacgaggtgt
    13921 tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg gcatattgat
    13981 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    14041 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgtgca agttcttcct
    14101 gccagttttc cagattaagg ccatgcacac tagtgagcag agccttcgag ttggcaacgc
    14161 cgcagacacg taagtcgata tgtttattct tcagccagct ttgctgacgc ttcagttgct
    14221 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    14281 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    14341 cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc
    14401 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    14461 ccaccgagat aatggccagc cgttccatca ccgccagcgg ctccagtaag ccttctttca
    14521 gttccaggta gaactcttcc tgcattgccc gttcagcccg cacacagtcg ctttgcggaa
    14581 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    14641 cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac cattcctttc atccccgggc
    14701 cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgc
    14761 cttcatcacg actggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa
    14821 tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    14881 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat
    14941 cgggcacctg acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac
    15001 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa
    15061 gtaccaccag ttcgcctttt tcattaccgg cggtgaagcc tgccatcagc accatgtgat
    15121 cagccggaat acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt
    15181 cgaggtaatg ccccactgcc agtagttttt cgaccggatc gataacggta acgttgtgac
    15241 cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca
    15301 gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact aatgccatgc aggacatgtt
    15361 ttatttgggc aaattcctgg tcgacgaaag ttttcaattg cgccagcggg aatcccggct
    15421 gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    15481 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt
    15541 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    15601 cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    15661 gcatggttgt tacctcgtta cctttggtcg aaaaaaaaag cccgcactgt caggtgcggg
    15721 cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg
    15781 tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta atttttatct
    15841 gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa taaaatttta
    15901 atttactcat ggcaggtaac cagttcagaa gctgctatca gacactcttt ttttaatcca
    15961 cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact tactaaggcg
    16021 ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac attgccgtgt
    16081 ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc gaccagtttt atgtcatctg
    16141 ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc agttgatgtt
    16201 ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat tgatagcaat
    16261 agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc ggcacaccag
    16321 taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca
    16381 ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcggcacc agttctactg
    16441 gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca gtgaaatcga
    16501 catcgtgtaa cgattcagcc agtgttggga aaactttaat attatcaata acatcaccag
    16561 atccatgtgc gacccagcgg gtggctggct ccaggtgtgc ctgactatcg acaatccgca
    16621 gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt tctgctctgg
    16681 cgggtgcgac caggataatc gttatacgca tattgccact cttcttgacc aaataaccgc
    16741 gaacagggtg atcactgtca acttattacg cggtgcgaat ttacaaattc ttaacgtaag
    16801 tcgcagaaaa agccctttac ttagcttaaa aaaggctaaa ctatttcctg actgtactaa
    16861 cggtttaatt gttaaaaaat gctacatatc cttctgttta cttaggataa ttttataaaa
    16921 aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa ctagttggat
    16981 tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca aactgttaac
    17041 gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt gtccggcccc
    17101 tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt gacactgtcg
    17161 ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg atggaaagtg
    17221 catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa tttgcagcat
    17281 gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc aatttaggta
    17341 gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac acgcaacacg
    17401 ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga tggcgcggaa
    17461 atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat caatctgccg
    17521 ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg
    17581 ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat
    17641 gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg caacctgctg
    17701 tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga aagctacaag
    17761 ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga tggcgagcag
    17821 tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa
    17881 attcagtccc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa accacacgac
    17941 cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac gccggatacg
    18001 ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga tctggaagat
    18061 taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
    18121 ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
    18181 ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc
    18241 accaccagca cactggttcc caacaacagc gcgctatcga cagagttgag cagtccccac
    18301 atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc
    18361 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
    18421 gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
    18481 gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
    18541 acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc
    18601 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcgag gaagttttcc
    18661 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
    18721 tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggcaa aaaatcacct
    18781 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg cccggcaggc
    18841 accacagaaa gatcgccggt accgcttaaa ttcagggcca tattcagctt caggttctgc
    18901 ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
    18961 gtgccgggtt caatggtcag cgccgttccg ttaacttcag gcgctttcac cacaccaata
    19021 ccacgcgcat ccccgacgct aatcacaata aatggcttgc caaaggtgat atttggcgcg
    19081 tcgagttcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
    19141 acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc atcaaccatc
    19201 aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt tttatcctct
    19261 tcctgtaccg tataaagctc ggtcacagga atagcgataa gcggcccaac aagtttttgc
    19321 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
    19381 acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacagg
    19441 gtggtcattt tccagaacag ggaggatttc aacatagtct tctcctttgc aatggcagca
    19501 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgaagc
    19561 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
    19621 gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
    19681 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
    19741 tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt tatacgaccg
    19801 ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg atccagtaac
    19861 gccggttcag cagcaacatt aacctcggtg ggcgtaacat gtagagtgat gtttttttct
    19921 gccaactgca cggtgcgcgc ttcgctgacg cggcggaata atgccgccac atcaacagca
    19981 gtcaaaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagt
    20041 gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
    20101 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
    20161 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
    20221 atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc gggaagagga
    20281 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat ggagcggttg
    20341 atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc ccaacaaaat ggcgctggcc
    20401 cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt cggtttgcct
    20461 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcacc
    20521 gaactttcgg gatcggcagg attttgcaac gtgctacgcg caccatactg accacgcaac
    20581 gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg
    20641 aacaatactt tgccctgcgc atcggtcata tagacatgat attcattgcg cactttgtta
    20701 atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
    20761 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt
    20821 gcggtatcga ttaacgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
    20881 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc cagcagcaac
    20941 cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga tgagtattaa
    21001 tcggagaaag atcggggttg atggcgcgca gcttggcacg caacgtttta atgtgggtat
    21061 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt agttgctggc
    21121 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
    21181 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
    21241 gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc agtaaggtgc
    21301 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
    21361 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
    21421 acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata tcaggcaaac
    21481 caacatcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc accggcaagc
    21541 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
    21601 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
    21661 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
    21721 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
    21781 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
    21841 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatagccg
    21901 catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt ttcgcccggc
    21961 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
    22021 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
    22081 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
    22141 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
    22201 caaaatctca atacttttat ttccgtcaag ccctaaaaca tactctacta agaaaaaaac
    22261 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
    22321 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
    22381 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
    22441 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
    22501 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
    22561 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
    22621 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
    22681 tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat ttgtcaccct
    22741 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
    22801 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
    22861 cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
    22921 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
    22981 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
    23041 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaacct
    23101 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
    23161 agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt aacgctgcag
    23221 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa cgacccagcc
    23281 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
    23341 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac atcccagtgc
    23401 aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
    23461 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
    23521 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
    23581 atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
    23641 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
    23701 gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
    23761 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
    23821 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
    23881 acgttaattt tctgagtaat gctgattatg caccaagtta tctgtgcgac caccaatccc
    23941 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
    24001 attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag tgaggaaacg
    24061 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
    24121 tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt ggtgattgaa
    24181 aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc ggtgattctg
    24241 cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
    24301 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc
    24361 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag
    24421 gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac gatgcctgat
    24481 gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc
    24541 cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt
    24601 cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc cacgcaacag
    24661 ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag
    24721 gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg
    24781 taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat
    24841 aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta ctcgctaaag
    24901 agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc cgtggcgctc
    24961 atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc cagcacgttc
    25021 ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata
    25081 cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct
    25141 gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta actggtgcca
    25201 atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag
    25261 aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc gctggcccct
    25321 accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag
    25381 ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg
    25441 aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc
    25501 caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt tgacttgctc
    25561 ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta catcaactcg
    25621 cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg cggcgcttta
    25681 tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg gtagaaacca
    25741 cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc gcgttccagc
    25801 aataaatcca cctgccagta acgccattca tctttctctt ttgcttccat cggcagacgc
    25861 gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg cacacggcgt
    25921 tcaatcaacg acgtggattg cgagcgcata atgacgtcat cgcgccattt cgcctgctcg
    25981 tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga
    26041 tcttcattaa gctgctgcac ctgggcaagc gacgggatca tcagccgtgc attttcagca
    26101 tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa atcggtcgct
    26161 ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc
    26221 gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggccagtg
    26281 ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct
    26341 gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc
    26401 cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag
    26461 tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca
    26521 cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga
    26581 ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcaga
    26641 tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc
    26701 atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa
    26761 cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag
    26821 acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct
    26881 aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt
    26941 gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact
    27001 gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc acgataaaca
    27061 tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg
    27121 ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa
    27181 ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt atggcgggca
    27241 ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttacctgc
    27301 cgcaggagcc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt
    27361 ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg
    27421 atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc
    27481 acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg
    27541 actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta gcgttgtgcc
    27601 gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg
    27661 ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc gtggtggcga
    27721 ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg
    27781 gtgaaggtat tccgtgggaa ggcaactact cctcctggct ggagcagaaa gatcagcgcc
    27841 tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt
    27901 gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg
    27961 aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg tttattccac
    28021 ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggtg
    28081 atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc gtcgggatca
    28141 tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt caggaacagc
    28201 cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcgtcggtt gatcagttcc
    28261 gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta
    28321 tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag
    28381 gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc
    28441 tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc
    28501 tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga
    28561 tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc
    28621 aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa gagtacaaga
    28681 aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag cgtattgcga
    28741 agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca aaccaagaaa
    28801 tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc atcaggcatt
    28861 gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaacag ccttagtatc
    28921 cgcaatttcg tagcgggtca cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc
    28981 ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc ctttgagccg
    29041 tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac gcagcgcagg
    29101 aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc
    29161 atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa
    29221 ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca
    29281 tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg
    29341 gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac tgcaaaatca
    29401 atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa tgcgatctca
    29461 tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact ggtggtattg
    29521 aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc accaaggatc
    29581 gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca gtagcggaac
    29641 gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg atcgaccagc
    29701 accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag
    29761 atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca
    29821 tcccagccgt gcggatacgg ctccatgccc tcttcgttga aagcatgaat gcgaatattt
    29881 ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt gggctgctgc
    29941 gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata
    30001 tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcagc
    30061 gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa ttcaagcccc
    30121 aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt ggcattcagt
    30181 aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg tagcgtgcag
    30241 ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct
    30301 ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct
    30361 gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac
    30421 cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg atctcccctg
    30481 ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca cgcaggctgg
    30541 aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc acgccgccga
    30601 ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg tgcaagacag
    30661 ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc ggattcgcca
    30721 tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca
    30781 ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa attgccgaag
    30841 ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg
    30901 cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca tcaccatcca
    30961 gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc gagccatctt
    31021 tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag cgcgtcagat
    31081 aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca tccgccatat
    31141 gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg gcaatcaggc
    31201 agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc
    31261 gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac agcgttttca
    31321 tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc gcacctgggt
    31381 taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg cggtcgaact
    31441 ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag agtttctgga
    31501 cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc gccaccgttg
    31561 gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc
    31621 cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt
    31681 ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg agcaattact
    31741 tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg
    31801 tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc agccctgctg
    31861 ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc gccagcggga
    31921 tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg tctacgatgt
    31981 cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg
    32041 cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg ccggaggcaa
    32101 tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag cctggcatca
    32161 gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca
    32221 cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttactt
    32281 ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat tcaatctgga
    32341 tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc gggattttcc
    32401 ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt gcggtgagtg
    32461 aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatcg
    32521 ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca cgaccataca
    32581 gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg
    32641 aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag
    32701 gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt
    32761 atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac
    32821 aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg ccttgttagt
    32881 ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca
    32941 gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat
    33001 gcgtaccgac tcaccagatg aaaaacgcat tcaggcgatc ctcgatcagt taacggatga
    33061 aagccgcatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg cccgttcagg
    33121 cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcag gcggctattt
    33181 taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct atctgcgcct
    33241 gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat
    33301 tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc gcacgctgct
    33361 acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt
    33421 gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt
    33481 tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt
    33541 ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa
    33601 tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg
    33661 gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg cggtgttcca
    33721 cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaaccat
    33781 ctctacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg
    33841 acgtaacgat ctggtggtga aaacagtcga aggcgaccgc aaagtctcag gctcggccta
    33901 tcgcgaaacc aaagatcgtg gcttccacca cggcactttg ctgctcaatg ccgaccttag
    33961 ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag gcattacctc
    34021 agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc atgagcaggt
    34081 ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag aagcggaaat
    34141 catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg cccgtcagag
    34201 tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg atgaacgctt
    34261 tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca cccgcgccca
    34321 ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc gactgcaagg
    34381 ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc
    34441 ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg taaggtaact
    34501 cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag gagcgggaga
    34561 ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt atcaggcata
    34621 aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct ctaaaataga
    34681 tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatgggagaa atttatggtt
    34741 gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa tgaatagatt
    34801 cgtgaataga tacttgaatg tattttattt tttatccata tgaaaaataa tagtaaatct
    34861 ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc ccactccggc
    34921 ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca gcagaacgga
    34981 ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg
    35041 ctcgtgagtg cggatgtggt cagacacagt gcagatggtc agggctttcg cgccaaattc
    35101 tgcagcgacg ccgtagatac cagccgcttc catttccacg ccgaggatgc cgtatttttc
    35161 catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga acaggttacc
    35221 aacgcgagca tcaatgccca gtgctttagc cgcgtctacc gcgttacgca ccatgtcaaa
    35281 gtcagcgata gcggcaaagt catgatcttt aaaacggatg cggttaactt tggaatcggt
    35341 gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa ctgcgccaca
    35401 ggaacccacg cggataattt tcttcacgcc gaaatcggtg atcagttctt tggtgtagat
    35461 ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc ctttgtaagt
    35521 accggtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt
    35581 ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc
    35641 gcccatttct gcattaatgt gtggggtagc cattgtttta tccttatcaa catgttttca
    35701 ccctctccct gagggagagg gccggggtga gggcatcaag ccgcacccat tatcagaaca
    35761 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    35821 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggccg taaaccagta
    35881 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcacaa ccgtggtcag
    35941 cagtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    36001 cgaacagctc cagacccaca gcataaccgg cgacgtcgcg acggtggccc caggaagagt
    36061 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    36121 tggtggcgtc aaacagcgcg tccaggccag ttgctttcac tttcttggtg ataccgcagt
    36181 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg accgtgtttt tcatcaacca
    36241 gtttctgcag cacggtcggt gctggcggct caacagccag gtcgtgacgg ttaccggtac
    36301 gctggaagtt accggctttg tcaccgataa acggacgagc gataacacga ccgatgttgt
    36361 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccaggc
    36421 caaaggtttc ttcgtggcag gcaatctgga acacagagtc agcggaggta tagaaaatcg
    36481 gcttgccggt tttcatgtgc tcttcgccca gctgatccag aatgaccgta ccggaagagt
    36541 ggcagttacc gaggtaaccc ggcagattag cgcgttcaac cagtttatcc agcagctctt
    36601 gcgggaagct gttttcgtga tcggagaaat atccccactc aaatagaacc gggacaccgg
    36661 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    36721 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    36781 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcgga cctttacgac
    36841 cgttatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    36901 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    36961 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    37021 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gctttatcgg
    37081 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    37141 cgtggataac cgccagcgga cgctgaccgt ctacctggtc gcccaaacgc gccatatcag
    37201 taaagccgac gctgtaatca atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    37261 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    37321 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    37381 ttgggccttt ttgtgccgct accatacgac caaagacttc tgctgcttta ccgttgtcca
    37441 gcactgcttg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagataa
    37501 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    37561 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    37621 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    37681 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    37741 tcacgtccat caccagcgcg tccagacctt ccgcaagttt cttcgccaga atagaggcgg
    37801 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    37861 ccgccggagc cagtgagctg gtctgaccga taatcgccac gccgacgtct ttaataattt
    37921 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    37981 gcgtaccgcc agtatgaccg aggccgcgac cagagatcat cggaatatag ccgccgcagg
    38041 ctgcgaccat cggccccaac atcagcgaag tcacatcgcc gacgccacca gtggaatgtt
    38101 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    38161 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    38221 tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc gcgaatgccg ttgataaaga
    38281 aacgaatttc ttcatcgctc agtgcatgac catcacgttt tttacgaata atttcttgtg
    38341 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    38401 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    38461 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcaccgtgg cccagcgctt
    38521 tcaacaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tctgcccagt
    38581 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    38641 cgccgcccgc tggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    38701 tcatgatgcg cgcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    38761 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    38821 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    38881 tacaggcttt caccaggtca aaaccaacct gctcgttacc cgccatcagc gcgcggtacg
    38941 ggaacacaac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    39001 cgatttcgat gtcatcgtta ccgtgtggga agttggttac cgtagcgatt cggatttccg
    39061 gggtgccctg ctctttcagc gttttgcgag caatcgggat aaagcgagga tagatacaga
    39121 tagcggcggt attgccgacc ggggttttgg cctgatgaca cagagcaatt actttctcgt
    39181 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    39241 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    39301 tgttagtatt ctaacatcta ctccgcaaca cacttcgata tacatcacaa ttaaggaaat
    39361 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    39421 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    39481 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    39541 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    39601 tttctggcac tggaagcaga aaacaatctg ccttatccgc agcttcctgc cgaagcccgc
    39661 cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    39721 cgctatgtct tacccgatta cgcccgtttt ctggcgaacg gttccgaatg gctggagctg
    39781 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    39841 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccatat
    39901 gttagaattc taacacaaga cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    39961 gacagaaccc tgccagacgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    40021 acccgcgcga tcttacgtgc agacgcagag ttgaagcagg tttcaccgaa cctgaccttt
    40081 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    40141 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    40201 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    40261 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    40321 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc
    40381 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    40441 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    40501 tgtaagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    40561 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    40621 caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg
    40681 ggcgcgcgtt tgccgtatgg tgatgagcca gatccgatca cccatctgca aactgtcgcc
    40741 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc
    40801 aaacgtaatc cgcaggcgct ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    40861 gaatttactg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    40921 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    40981 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    41041 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    41101 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    41161 gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    41221 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtt tgcgagcagt gtgatacctg
    41281 cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa tccatgagcg tggacgaagt
    41341 gcttagccat gtccgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    41401 agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    41461 actgcgccac ctcacctgcc tggtggacag taacggcatg ttgagcgaaa ccggctggga
    41521 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcgtggg ggagcgaatg
    41581 tcatcaacac ttaaccggac gcgataatca gcagattaag cgcagcatct gtttgcttgc
    41641 agagcgcggc aagctggcgg aactgcgttt gctggtaatt ccagaccagg tggattattt
    41701 gcatcacatc gatgagctgg cgacgtttat caagagactt ggcgatgttc cggttcgcct
    41761 gaatgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    41821 ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc ggggtgagcc ggttgatatt
    41881 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    41941 atgataagac gcgacaagcg tcgcatcagg cattatgcgc cgactgccgg atgcggcgtg
    42001 aacgccttat ccggcctacg gttcggcaca gacttgtagg catgataaga cgcgtcagcg
    42061 tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg aacgccttat ccggcctacg
    42121 gttcggcaca gacttgtagg catgataaga cgcgtcaagc gtcgcatcag gcattgtgcg
    42181 ccaactgccg gatgcggcgt gaacgcctta tccggcctac ggttcggcac aaacctgtag
    42241 gcatgataag acgcgtcaag cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg
    42301 tgaacgcctt atccggccta cggttcggca caaacctgta ggcatgataa gacgcgttaa
    42361 gcgtcgcatc aggcattatg cgccgactgc cggatgctgc ctacgaaggg taggctaccc
    42421 actaacgcta aacaacgcat acgtattatt aagcaacact tcggcaatct catcttctgg
    42481 ttccgggcgc aactcgcaaa gcacgtcaaa cacccgcaca gcctgttccg gtcggttagg
    42541 ctgcccctga aagccgttga gcggcatatc cggcgcgtcg gtttccagca acaacgatgc
    42601 cagcggtaat tttgcgatga catcgcgggt tttactggcg cgtggatagg tgatagtacc
    42661 gcctacgccg attttgtagc ccagctgtac aaaccgctcg gcctgttgca ggctgccgga
    42721 aaaaccgtga accacgccag tgcacggtaa atcgtgacgt ttaagatgca tcgccagttt
    42781 gtcgtgtata cgccgggaat gaaggatcac cggcagatcg tagcgtttcg ccagtttcag
    42841 ctgttcgtcg agtaaccact gctgcctctc aaattgcgga tcgtcgccga agagatccag
    42901 accgatctcc cccaccgcca ccaccttcgc cggacgcctt tccagcgcct gctgtagctg
    42961 atcaagagac acatcgctat gtttttccaa catccccgga tgcaacccca atgcggcata
    43021 cagcggttga tatttttccg ctaacgcctg cacgcgggca aaattttccg cctctgtcgc
    43081 cggaacaatg atcttgccta cgcccgcttg tgccgcgcgt tgcaggctgg cctcttcatc
    43141 gccactaaac ggcgggaaat caaaatggca gtgggtatcg ataaaacggc aaatcaagcc
    43201 agatcctcat tgttaaatgt cgggtcgttc gcctgcggag catcgctcac ttcggcaacc
    43261 agcgtatcgt tggcgactgg cgcaggcgga ataacgatcg attccggcgt caccactggc
    43321 accagatggc gggtaagcgg cgctttttca gttaatagct tgccaaccgt ggcgaggaaa
    43381 taacgaccgc ataaacgccc aagtttatag tcttcacgca gcgccggaat ccgactgcca
    43441 agcgcgatac tatgtaatgg cttcggcgga taaatttcga atatccgcag cttgcccggt
    43501 ggtttctcaa taaattgctg aatgtcacga tagctggttt catgatgctg caccagattg
    43561 accagcggct gcaggctact gtcacccagc cagcgttcca tgcgtttgaa ccactgcggc
    43621 gtgtagtaca tttgtgacgg cacagtgcga atgacgacca acgttttagc gccctgcctt
    43681 gccgcctctt taaccggaat cgcatcactg atcccgccat ccaggtagtt aatgccttcc
    43741 agcgacactc cgctacgata aaagccaggt atcgcactgg aggcgcgaat cacatccagc
    43801 cagttttgtt tggttggtaa aaagtaatta ggcgcgtagt catcctgacg acaggcgcac
    43861 atataaaacg atttgccgct gtcaaacaac cgcgcggcgg tatccatttg caacggcatc
    43921 tggcttgcgg tggcctccac cagccagtcg agatcgataa gatttcctcc acggacaaag
    43981 cgcaatggat cgaaaaattc gcgttttgtg gtatagcgca tgatgacttt gcgcgcgtaa
    44041 ccgggctgat tgcagataaa cgccgagagg ttctgcgccc cggcagatgt gccgagataa
    44101 agatcgaaag gattaaactg cgcgcgcata aactcatcca gcacgccagc cgtgaaaatt
    44161 ccacgctgtc cgccgccttc gcacaccaga gctattcgtc caggctggaa cggccttagc
    44221 gacaacggcg caatattacc aagcgttaca ggtattcgct gccccacctc tgccttcctg
    44281 tttttgttgt gaaagaatga caaagtaacg caatttatta ttcgctaaaa ccgcaaaagc
    44341 cagtccgctg gactggcttt ggataaaatt ttcttaatgg tctgaaatct agggtcgttt
    44401 tcggcccatg aacaaactca ccaggaacag aataatcccg acgacaaaga caattttagc
    44461 tgcgcctgca gcggtaccgg ccagaccacc aaacccaagt gcggcggcga ttaacgcgat
    44521 aaccagaaat atgatgcccc aacgaaacat aagcgtctcc tttaccatag ttaatgtcac
    44581 cgcttgatat gagcgtgaaa atcactcaat gcgatatttt tagtgtggtg cacatcgcgc
    44641 ctcccgacaa agttcgggag gacgaattac gacgaattac ttagttttca gatcattttt
    44701 aacgcttttc acaccatcta ccgctttggc gatactttca gcacggtcgc tctgtgcctg
    44761 ggaatcgacg gtaccggaga gctgaaccac gccgtcggtg gtttcaactt tcacatgacg
    44821 ggaagggacg atatcgtccg ccagcagttt ggctttgatt tcactggtgg tggcggtgtc
    44881 acccgcgtag cccttcacag agccttcttt agcgtcgcga acgtgcagtt tgtcgctgac
    44941 agaggtcacc ccttcaacgc ctttcgccac tttcactgcc tcttcggcct gagcctggct
    45001 ttcaacgaaa ccgctcaggg tcacgacttt ttgatcggtt ttgacagaga tatcggtgct
    45061 cttgatgttg tcatgatcca ccagcgccgc cttcactttc gcggtgatgg cgctgtcatc
    45121 catgaaatta ccgactttat tcatagagct atcgactttt tgccctgcgc tttcattggt
    45181 agtctgtgcg ttgttttccg cgtaggcaga gccggtcgcg acggcagagg tcaacattac
    45241 agccagcaga gttttcgaaa tcttcagtct tgtcatagtc atcgatttat tcctgtatgt
    45301 ttgctcgtaa tttgagcctg gcaacacgag gttgcattgc tgaataggga gagacttcac
    45361 cctctacaga agtcaatggt cgccatcaca aaagcgatgg gtgatgaata acgaccatta
    45421 cagcctctga atcagttatt aatatcggta gaatgacaat cgacggcttt agatactgat
    45481 atctacgcat tgaacggtat ttaacgccgt cagaaatgtc attactttgt taaatataga
    45541 tcacaatttt gaaaccgctc gggatatcag cgaaaacata agcaaaagtg aatgttttaa
    45601 gaacattccg taagcggcta ataaggaagg gaaattgaca gggcgcagcg gctaccgcgc
    45661 cctggaggca agaattaatg ctcgcgggtc tggtggaact gaacgtcggg ataacgttcc
    45721 tgcgccaggc gcaggttgac catgctggta gcgatgtaag cgaggttatc gccgccatca
    45781 agcgccagtt ggctttcgtt cttacgcttg aactcttcga atttcttcgc gtccgcacat
    45841 tctacccagc gggcagtggc aacgttgact gattcatata ctgcttcaac gctgtattcg
    45901 ctcttcaggc gcgataccac cacatcaaac tgcagcacac caacagcacc aacgatcaaa
    45961 tcgttgttgg agatcggacg gaacacctgc accgcgccct cttcggaaag ctgtaccagc
    46021 cctttgagca gctgtttttg cttcagcgga tctttcaggc ggatacgacg gaacagttct
    46081 ggtgcgaagt tcggaatacc ggtgaacttc atcatctcac cctgggtaaa ggtgtcgccg
    46141 atctgaatag tgccgtggtt gtgcaggccg aggatatcgc ccggatacgc ttcttcaacg
    46201 tgcgaacggt cgcccgccat aaaggtcagc gcgtcggaga taaccacatc tttcgcagta
    46261 cgcacctggc gcagcttcat gcctttttca tatttaccgg atactacgcg cataaacgcc
    46321 acgcggtcgc ggtgtttcgg gtccatgttg gcctgaattt taaatacgaa gccggtaaat
    46381 ttgtcttcgc tcgcctctac ggtacgggta tcagtctgac gcggcatcgg cgcaggagcc
    46441 cactccacca ggccatccag catatgatca acgccgaagt tacccagcgc agtaccgaag
    46501 aataccggag tgatttcgcc cgcaaggaac aattctttgt cgaactcgtt agacgcgcct
    46561 ttcaccagtt ccagttcgtc acgtagctgc tgtgccagat cttcaccaac cgcagcgtcg
    46621 agatccgggt tattcagccc tttaacaata cggacttcct gaatcgtgtg gcctttaccg
    46681 ctctgataga gataggtttc gtctttataa aggtggtaaa cgcctttaaa cagcttgccg
    46741 cagccaatcg gccaggtgat cggtgcgcag ccgattttca gctcgttctc aacttcatcg
    46801 agcagttcca tcgggtcgcg gatatcacgg tcaagtttgt tcataaaggt gaggatcggc
    46861 gtgtcgcgca gacgggtaac ttccatcagc ttacgggtac gatcttcaac accttttgca
    46921 gcgtcgataa ccatcaggca gcagtccacc gccgtcaggg tacgataggt atcttccgag
    46981 aagtcttcgt gccccggggt gtcgagcagg ttaaccaggc aatcgtgata cggaaactgc
    47041 atcacagacg tagtaatgga gatcccacgc tgcttttcca tctccatcca gtccgactta
    47101 gcgtgctggt tggaaccacg gccttttact gtaccggcgg tctgaatggc ctgtccgaac
    47161 agcagcacct tctcggtgat ggtagtctta ccggcatccg ggtgagaaat aatggcaaaa
    47221 gtgcggcgct tcgccacctc ttgcaaataa ggagacaacg tcataattct ttcttcttga
    47281 gtaagcggca tcgcgccgcg cttgttgaaa acgaaaaatt gcggctattt tacccatcaa
    47341 cgggggggag gcaatcagtg tttacacagg agctgctcca gttcgtgcaa cgaagaaacg
    47401 gtccaggtgg gcgcgatgcc ttctggttgc tcgcgattgt gcgcattcag ccagcaggtc
    47461 gcaagcccgg cgttgatgcc accgagaata tcggactcgg cagtgtcgcc aaccatcagc
    47521 acgcgtgaac ggtcaggatt gcccgcctgt tccagcgcat aatcgaaaat tttcttattc
    47581 ggtttggcaa cgccaacttc ttcggaaatc accagcaaat cgaagtaatc acgcaggccc
    47641 gtgcgttcca gacgcacttg ctgcaaggcg ctaaagccgt tggtgatgat gccgattttg
    47701 gcgttgccac gaatggcgtt aagcagagaa accgcgcccg gcagcggcgt gcagatttcc
    47761 gccatcgcat taataaaggc atcgttaagc aagcctgctt caactttcag ccgttcggcc
    47821 cagctctcaa aacgcccgtg ctgaagctgt aatgaagtga tcgcgccgtt ttgataatcc
    47881 acccacagtg gcttgttaac ggcctgatag tcctgaaaat cttcagcggt aaaggtgacg
    47941 ctgtaatcaa gaaacatccg ctgcaggccg gtgaatgagt caaaggtaaa cagcgtttca
    48001 tcggcatcaa agaaaatcca gtcccacttc attattccac cttgtcttac atactgattg
    48061 gcaacgccat gatgatggcg tcttcgcgac cgtccgtggt ggggtagtaa ttgcggcgaa
    48121 tcgtcgcctc gttaaagcct aaactttcgt acagggcaat ggcggcagca tttgaggcac
    48181 ggacttccag ccatagtgtc gccacgccgc gtttttccag ttcgtcgatc agatgttcca
    48241 gcagcaccct tcccaatccc tgacgctgat agtcaggatc gaccgcaata ttgaacaatg
    48301 tagcttcatc cagcaccact tgcgtaatcg caaacgccgc cattttgccg ttttgcgtta
    48361 actgaaagtt gagataacgc tcgccctggt tgctggcaaa ggttttttca ctccacggaa
    48421 aggcgtgggc gcgttgttca atgtggtaag ccgctggtaa atcagtcgtt tcgagggaag
    48481 aaatcgtgtt catatgtgca aatctgttgc cataacgcgg cgcgtgccgt tgggtttgcc
    48541 cgtaattcgg tgagcgccgg tgatgccacc tgagcgcctt ccagtgatag cggttcgtca
    48601 gtacccaacc gccagctgtt gcagcgactg ccttgcggca gcatcgcgat tttttctggc
    48661 gtcagttgca gcacttgatc ggggctgacg gttaatgcgc gcagaacatc gctcactaaa
    48721 ggatcggtca gggcgggaag atcgtttgcc accatcacca gacggacgtg tgccgggatc
    48781 gcaatggcaa tctccccctg caacgcgcca gggcgacgca gcgaccactg ggtaatgccc
    48841 agttgctgta actgccagtc tcgtcgggat gtcatagcga atcgctcctg ttgtcagggg
    48901 cgcaaatata gcaaattcgt cgataccgcg ccaacatatg gctataatcg ccgccagtat
    48961 caattgagga gcattccatg tctgcattta ccccggcaag tgaagtcttg ctgcgtcaca
    49021 gtgatgattt cgaacaaagc cgtattctgt ttgccggaga cttacaggat gacctgcccg
    49081 cgcgtttaga taccgcggcc agccgtgctc atacccagca attccaccac tggcaggtat
    49141 taagccgcca gatgggggat aacgcccgtt tcagtctggt cgccacggtg gatgacgtcg
    49201 cagattgcga tacgctgatt tactactggc cgaagaacaa accggaagcc cagttccagt
    49261 tgatgaattt actttctctg ctgccagtgg ggacagatat ttttgtcgtt ggcgagaacc
    49321 gcagcggcgt gcgcagcgcc gagcagatgc tggcagatta tgcgccgttg aataaagtcg
    49381 acagcgctcg tcgctgtggc ctctattttg gtcgtctgga aaaacagccg gtatttgatg
    49441 ccgataaatt ctggggcgaa tacagcgtcg atggcctgac ggtcaaaacg ctgcctggcg
    49501 tgtttagccg cgacggtctg gatgtcggta gccagttgct gctctcgacg ttaactccgc
    49561 acacgaaagg taaagtgctg gatgtcggct gtggcgcggg ggtgctttca gttgcctttg
    49621 cgcgccattc gccgaaaatt cgtctcacct tgtgcgatgt ctctgcgcca gcggtagaag
    49681 ccagccgcgc aacacttgcg gccaactgtg ttgaaggtga agtctttgcc agcaacgtct
    49741 tttccgaggt gaaaggtcgt tttgatatga tcatctccaa cccgccgttc cacgatggga
    49801 tgcaaaccag cctggatgcg gcgcaaacgc tgattcgcgg cgcggtgcgt catcttaata
    49861 gcggcggcga gctgcgaatt gtagcgaacg ccttcctgcc ttacccggac gtgctggatg
    49921 agacatttgg cttccatgaa gtgattgcgc aaaccgggcg tttcaaggtg tatcgcgcca
    49981 ttatgacccg ccaggcgaag aaaggttaat tatctcgccg aataccgtgt cggatgcggc
    50041 gtgaacgcct tatccgacct acaaaacctg cacgttagcc cttcgtaggc cagataagac
    50101 gcgccagcgt cgcatctggc atctgccccc gcatgcctac caaacctgca cgctagtttc
    50161 ctgatggaca tttttccagc aattacacct ccggcgataa ttaactattg acgaaaagct
    50221 gaaaaccact agaatgcgcc tccgtggtag caattctttt taagaattga tggtatgcga
    50281 aggtggcgga attggtagac gc
//
LOCUS       DAZPBM010000009        49317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPBM010000009 DAZPBM010000000
VERSION     DAZPBM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.009"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            288..1382
                     /gene="gcvT"
                     /locus_tag="ACHUZQ_000451"
     CDS             288..1382
                     /gene="gcvT"
                     /locus_tag="ACHUZQ_000451"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1024245.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            1406..1795
                     /gene="gcvH"
                     /locus_tag="ACHUZQ_000452"
     CDS             1406..1795
                     /gene="gcvH"
                     /locus_tag="ACHUZQ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1024246.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1914..4787
                     /gene="gcvP"
                     /locus_tag="ACHUZQ_000453"
     CDS             1914..4787
                     /gene="gcvP"
                     /locus_tag="ACHUZQ_000453"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1024247.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5053..5796
                     /locus_tag="ACHUZQ_000454"
     CDS             5053..5796
                     /locus_tag="ACHUZQ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1024248.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5853..7286)
                     /gene="bglA"
                     /locus_tag="ACHUZQ_000455"
     CDS             complement(5853..7286)
                     /gene="bglA"
                     /locus_tag="ACHUZQ_000455"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1024249.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            7331..7642
                     /gene="yqfB"
                     /locus_tag="ACHUZQ_000456"
     CDS             7331..7642
                     /gene="yqfB"
                     /locus_tag="ACHUZQ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR1024250.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7806..8465
                     /locus_tag="ACHUZQ_000457"
     CDS             7806..8465
                     /locus_tag="ACHUZQ_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1024251.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8661..9641)
                     /gene="ygfZ"
                     /locus_tag="ACHUZQ_000458"
     CDS             complement(8661..9641)
                     /gene="ygfZ"
                     /locus_tag="ACHUZQ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1024252.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9884..10150
                     /gene="sdhE"
                     /locus_tag="ACHUZQ_000459"
     CDS             9884..10150
                     /gene="sdhE"
                     /locus_tag="ACHUZQ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1024253.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10131..10538
                     /locus_tag="ACHUZQ_000460"
     CDS             10131..10538
                     /locus_tag="ACHUZQ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1024254.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10578..11099)
                     /gene="fldB"
                     /locus_tag="ACHUZQ_000461"
     CDS             complement(10578..11099)
                     /gene="fldB"
                     /locus_tag="ACHUZQ_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1024255.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11211..12107
                     /gene="xerD"
                     /locus_tag="ACHUZQ_000462"
     CDS             11211..12107
                     /gene="xerD"
                     /locus_tag="ACHUZQ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1024256.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12132..12842
                     /gene="dsbC"
                     /locus_tag="ACHUZQ_000463"
     CDS             12132..12842
                     /gene="dsbC"
                     /locus_tag="ACHUZQ_000463"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1024257.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12848..14581
                     /gene="recJ"
                     /locus_tag="ACHUZQ_000464"
     CDS             12848..14581
                     /gene="recJ"
                     /locus_tag="ACHUZQ_000464"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1024258.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14672..15770
                     /gene="prfB"
                     /locus_tag="ACHUZQ_000465"
     CDS             join(14672..14746,14748..15770)
                     /gene="prfB"
                     /locus_tag="ACHUZQ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1024259.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15780..17297
                     /gene="lysS"
                     /locus_tag="ACHUZQ_000466"
     CDS             15780..17297
                     /gene="lysS"
                     /locus_tag="ACHUZQ_000466"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1024260.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17340..17888)
                     /gene="idi"
                     /locus_tag="ACHUZQ_000467"
     CDS             complement(17340..17888)
                     /gene="idi"
                     /locus_tag="ACHUZQ_000467"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1024261.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17943..18014
                     /locus_tag="ACHUZQ_000468"
     CDS             17943..18014
                     /locus_tag="ACHUZQ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024262.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(18011..18136)
                     /gene="yqfG"
                     /locus_tag="ACHUZQ_000469"
     CDS             complement(18011..18136)
                     /gene="yqfG"
                     /locus_tag="ACHUZQ_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1024263.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18138..19216)
                     /locus_tag="ACHUZQ_000470"
                     /pseudo
     CDS             complement(18138..19216)
                     /locus_tag="ACHUZQ_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            19652..21571
                     /gene="ygfT"
                     /locus_tag="ACHUZQ_000471"
     CDS             19652..21571
                     /gene="ygfT"
                     /locus_tag="ACHUZQ_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR1024264.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21571..22059
                     /locus_tag="ACHUZQ_000472"
     CDS             21571..22059
                     /locus_tag="ACHUZQ_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1024265.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22128..22478)
                     /gene="ghxQ"
                     /locus_tag="ACHUZQ_000473"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(22128..22478)
                     /gene="ghxQ"
                     /locus_tag="ACHUZQ_000473"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(22514..22828)
                     /locus_tag="ACHUZQ_000474"
     CDS             complement(22514..22828)
                     /locus_tag="ACHUZQ_000474"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1024266.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(22761..25700)
                     /locus_tag="ACHUZQ_000475"
     CDS             complement(22761..25700)
                     /locus_tag="ACHUZQ_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR1024267.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(25697..26476)
                     /gene="ygfM"
                     /locus_tag="ACHUZQ_000476"
     CDS             complement(25697..26476)
                     /gene="ygfM"
                     /locus_tag="ACHUZQ_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR1024268.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(26527..27855)
                     /gene="ssnA"
                     /locus_tag="ACHUZQ_000477"
     CDS             complement(26527..27855)
                     /gene="ssnA"
                     /locus_tag="ACHUZQ_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR1024269.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(27858..30956)
                     /gene="ygfK"
                     /locus_tag="ACHUZQ_000478"
     CDS             complement(27858..30956)
                     /gene="ygfK"
                     /locus_tag="ACHUZQ_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR1024270.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(31278..31856)
                     /gene="mocA"
                     /locus_tag="ACHUZQ_000479"
     CDS             complement(31278..31856)
                     /gene="mocA"
                     /locus_tag="ACHUZQ_000479"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR1024271.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            31959..32729
                     /gene="yqeC"
                     /locus_tag="ACHUZQ_000480"
     CDS             31959..32729
                     /gene="yqeC"
                     /locus_tag="ACHUZQ_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR1024272.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            32777..34402
                     /gene="yqeB"
                     /locus_tag="ACHUZQ_000481"
     CDS             32777..34402
                     /gene="yqeB"
                     /locus_tag="ACHUZQ_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR1024273.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(34443..35375)
                     /gene="arcC"
                     /locus_tag="ACHUZQ_000482"
     CDS             complement(34443..35375)
                     /gene="arcC"
                     /locus_tag="ACHUZQ_000482"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1024274.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(35423..36808)
                     /gene="hyuA"
                     /locus_tag="ACHUZQ_000483"
     CDS             complement(35423..36808)
                     /gene="hyuA"
                     /locus_tag="ACHUZQ_000483"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR1024275.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(36861..38072)
                     /locus_tag="ACHUZQ_000484"
     CDS             complement(36861..38072)
                     /locus_tag="ACHUZQ_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR1024276.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(38130..39326)
                     /gene="dpaL"
                     /locus_tag="ACHUZQ_000485"
     CDS             complement(38130..39326)
                     /gene="dpaL"
                     /locus_tag="ACHUZQ_000485"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR1024277.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(39384..40571)
                     /gene="ygeW"
                     /locus_tag="ACHUZQ_000486"
     CDS             complement(39384..40571)
                     /gene="ygeW"
                     /locus_tag="ACHUZQ_000486"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR1024278.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            41050..42828
                     /locus_tag="ACHUZQ_000487"
     CDS             41050..42828
                     /locus_tag="ACHUZQ_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR1024279.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(42868..43347)
                     /gene="xdhC"
                     /locus_tag="ACHUZQ_000488"
     CDS             complement(42868..43347)
                     /gene="xdhC"
                     /locus_tag="ACHUZQ_000488"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1024280.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(43344..44222)
                     /gene="xdhB"
                     /locus_tag="ACHUZQ_000489"
     CDS             complement(43344..44222)
                     /gene="xdhB"
                     /locus_tag="ACHUZQ_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1024281.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(44233..46530)
                     /gene="xdhA"
                     /locus_tag="ACHUZQ_000490"
     CDS             complement(44233..46530)
                     /gene="xdhA"
                     /locus_tag="ACHUZQ_000490"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR1024282.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            46945..47700
                     /gene="actS"
                     /locus_tag="ACHUZQ_000491"
     CDS             46945..47700
                     /gene="actS"
                     /locus_tag="ACHUZQ_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1024283.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            47779..47848
                     /locus_tag="ACHUZQ_000492"
     tRNA            47779..47848
                     /locus_tag="ACHUZQ_000492"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47811..47813,aa:Gly,seq:ccc)
     gene            48053..>49317
                     /locus_tag="ACHUZQ_000493"
     CDS             48053..>49317
                     /locus_tag="ACHUZQ_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR1024284.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        11965 a        12178 c        13008 g        12166 t
ORIGIN      
        1 ttttagctct taatattgcc ttattcaaat tagctttcta atggcatcat ctttatatag
       61 aaactggtgt gttttttggt tttttattct gtcgcgattt tggcgttttt taaccataag
      121 ctaatgtgat gatcaatttt accttatggt taacagtctg tttcggtggt aagttcaggc
      181 aaaagagaac gattgcgttg gggaccggga gtggctccga tgctgggttt cgtggtgata
      241 atttcaccat gaaaagttgt cagccccgct tattcaatga ggacaagatg gcacaacaga
      301 ctcctttgta cgaacaacac acgctttgcg gcgctcgcat ggtggatttc cacggctgga
      361 tgatgccgct gcattacggt tcgcaaatcg acgaacatca tgcggtacgt accgatgccg
      421 gaatgtttga tgtgtcacat atgaccatcg tcgatctccg cggcagccgc acccgggagt
      481 ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac caaaagcggc aaagcccttt
      541 actcggggat gttgaatgcc tctggcggtg tgatagatga cctcatcgtc tactacttta
      601 ctgaagattt cttccgcctc gttgttaact ccgccacccg cgaaaaagac ctctcctgga
      661 ttacccaaca cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac ctttccatga
      721 ttgccgtgca agggccgaat gcgcaggcaa aagctgccac actgtttaat gacgcccagc
      781 gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca ggcgggcgat ctgtttattg
      841 ccaccactgg ttataccggt gaagcgggct atgaaattgc gctgcccaat gaaaaagcgg
      901 ccgatttctg gcgtgcgctg gtggaagcgg gtgttaagcc atgtggcttg ggcgcgcgtg
      961 acacgctgcg tctggaagcg ggcatgaatc tttatggtca ggagatggac gaaactattt
     1021 ctcctttagc cgccaacatg ggctggacta tcgcctggga accggcagat cgtgacttta
     1081 tcggtcgtga agccctggaa gtgcagcgtg agcatggtac agaaaaactg gttggtctgg
     1141 tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt acgctttacc gatgcgcagg
     1201 gcaaccagca tgaaggcatt atcaccagcg gtactttctc cccgacgctg ggttacagca
     1261 ttgcgctggc gcgcgtgctg gaaggtattg gcgaaacggc gattgtgcaa attcgcaacc
     1321 gtgaaatgtc ggttaaagtg acaaaacctg tttttgtgcg taacggcaaa gccgtcgcgt
     1381 gatttacttt tttggagatt gattgatgag caacgtacca gcagaactga aatacagcaa
     1441 agaacacgaa tggctgcgta aagaagccga cggcacttac accgttggta ttaccgaaca
     1501 tgctcaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg gcgcaacggt
     1561 tagcgcgggc gatgactgcg cggttgccga atcagtaaaa gcggcgtcag acatttatgc
     1621 gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc cggaactggt
     1681 gaacagcgaa ccgtatgcag gtggctggat ctttaaaatc aaagccagcg atgaaagcga
     1741 actggaatca ctgctggatg cgaccgcata cgaagcattg ttagaagacg agtaacggct
     1801 ttattcctct tctgcgggag aggatcaggg tgaggaaaat ttatgcctca ccctcactct
     1861 cttcgtaagg agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac
     1921 agacgttaag ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg
     1981 ccgcgcaaca gcaagaaatg ctgaatgccg ttggcgcaca atcgttaaac gcgctgaccg
     2041 gccagattgt accgaaagat attcagcttg cgaccccgcc gcaggttggc gcaccggcga
     2101 ccgaatacgc cgcactggca gaactcaagg ctattgccag tcgcaataaa cgcttcacgt
     2161 cttacatcgg catgggttac accgccgtgc agctaccgcc ggttatcctg cgtaacatgc
     2221 tggaaaatcc gggctggtat accgcgtaca ctccgtatca acctgaagtc tcccagggcc
     2281 gccttgaagc actgctcaac ttccagcagg taacgctgga tttgactgga ctggatatgg
     2341 cctctgcttc tcttctggac gaggccaccg ctgccgccga agcaatggcg atggcgaaac
     2401 gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc ttccgatgtg catccgcaaa
     2461 cgctggatgt ggtccgtact cgtgccgaaa cctttggttt tgaagtgatt gtcgatgacg
     2521 cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct gttacagcag gtaggcacta
     2581 ccggtgaaat tcacgactac actgcgctta ttagcgaact gaaatcacgc aaaattgtgg
     2641 tcagcgttgc cgccgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg
     2701 cggatattgt ttttggttcg gcgcaacgct tcggcgtgcc gatgggctac ggtggcccac
     2761 acgcggcatt ctttgcggcg aaagatgaat acaaacgctc aatgccgggc cgtattatcg
     2821 gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc
     2881 aacatattcg tcgtgagaaa gcgaactcca acatttgtac ttcccaggta ctgctggcaa
     2941 acatcgccag cctgtatgcc gtttatcacg gcccggttgg cctgaaacgt atcgctaacc
     3001 gcattcaccg tctgaccgat attctggcgg cgggcctgca acaaaaaggt ctgaaactgc
     3061 gccatgcgca ctatttcgac accttgtgtg tggaagtggc cgacaaagcg ggcgtactga
     3121 cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcc gtggggatca
     3181 cccttgatga aaccaccacg cgtgaaaacg taatgcagct tttcagcgtg ctgctgggcg
     3241 ataaccacgg gctggagatc gacacgctgg acaaagacgt ggctcacgac agccgctcta
     3301 tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctacc
     3361 acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga
     3421 atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga
     3481 tcccaatcac ctggccggaa tttgccgaac tgcacccgtt ttgcccgccg gaacaggctg
     3541 aaggttatca gcagatgatt gcgcagctgg ctgactggct ggtgaaactg accggttacg
     3601 acgccgtttg tatgcagccg aactccggcg cacagggcga atacgcgggc ctgctggcga
     3661 ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt
     3721 ctgcgcacgg aactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg
     3781 cgtgtgataa aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg
     3841 gcgataacct ctcctgtatc atggtgactt atccttctac ccacggcgtg tatgaagaaa
     3901 cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg
     3961 cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt tattggcgcg gacgtttcgc
     4021 acctcaacct gcataaaact ttctgcattc cgcacggcgg tggtggtccg ggtatgggac
     4081 cgatcggtgt gaaagcgcat ctggcaccgt ttgtaccggg tcatagcgtg gtgcaaatcg
     4141 aaggcatgtt aacccgtcag ggcgcagttt ctgcggcacc gttcggtagc gcctctatcc
     4201 tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc
     4261 aggtggcaat cctcaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc
     4321 tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga
     4381 aagaagaaac cggcatcagc gagctggata ttgccaagcg cctgatcgac tacggtttcc
     4441 acgcgccgac gatgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg
     4501 aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaattg
     4561 accaggtgaa agccgctgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca
     4621 ttcagagcga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcggtattcc
     4681 cggcaggcgt ggcagacaaa tactggccga cggtgaaacg tctggatgat gtttacggcg
     4741 accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga
     4801 ctattttcta aaggcgcttc ggcgcctttt tagtcagatg acaaagtaca aaagtgctca
     4861 gacagtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc cggcactggc
     4921 gcgaatattt accctcaccc cggccctctc cctgaaaggg cgaggggaaa agcgtgccaa
     4981 cattgaagat tgagccagtt tgttagcaat ctcaaagata cgtcaacgaa ttaatttttc
     5041 tcggaaaaac aaatggctat agcacttgtg accgatggca gtcgcggcat cgggcgggca
     5101 actgcattac tgttggcgaa agaagagtat acggtggcgg ttaattatca acaaaatctc
     5161 catgcggcgc aggaagtggt gaacttaatc acgcaagccg gtggcaaggc attcgtgctc
     5221 caggcggata tcagcgacga aaatcaggtc atcgcaatgt ttacagcaat cgatcagcac
     5281 gatgaaccgc tagcagcgct ggtcaataac gccgggatct tgtttaccca gtgcaccgtt
     5341 gaaaacctta ccgcagagcg aatcaaccga gtactttcca ccaacatgac gggatatttt
     5401 ctctgctgcc gcgaggcggt aaaacgcatg gcgcttaaaa atggtggcag tggcggcgct
     5461 atcgtcaatg tctcttcggt ggcctcacgg ttgggttcgc caggggaata tgttgattac
     5521 gcggcatcga aaggggcgat tgatacgtta accaccggac tatcgctgga agtcgccgcg
     5581 cagggggtcc gcgttaactg cgtgcggcca gggtttattt ttaccgaaat gcacgccagc
     5641 ggcggcgagc ctggacgcgt cgatcgcgtt aagtcgaaca tccccatgca gcgtggtgga
     5701 caggcagaag aggtcgcgca ggccattgtc tggctactaa gtgataaagc ctcttacgtc
     5761 acgggaagtt ttatcgattt ggcgggcggg aaataaaaca gggaagttgt ctgaccggat
     5821 gcaacaagta ttgcatccgg tacttcatcg acttaaagct tctcgccgtt gctggtaatc
     5881 acctctttgt accagttaaa gctcttctta cgtgaacgcg acatatcgcc agtaccgtcg
     5941 tcatgtttat tcacatagat aaagccgtag cgtttgctgt actgcccggt ggtgaacgat
     6001 acgcagtcga tgcagcccca cggtgtgtag cccatcagat ccacgccatc gtaagtcacc
     6061 gcttttttca tctcttcgat atgggcgcgc aggtagtcaa tgcggtagtc gtcgttgatg
     6121 ctgccatctt cttccacttt gtcgtaagcg ccaaaaccgt tttcgacaat aaacagcggc
     6181 ctctgataac gctcatacag ttcgcaaagg gcatagcgca gacctactgg atcaatctgc
     6241 cagccccagt cagacgcttt aacatacggg ttcggtacgc tgccttcgaa accagagatc
     6301 gcatcgccgg tgccgccttc ggccttcact gcgttggtca tgtaatagct gaaaccaaga
     6361 taatcgcagg tgccttcgcg cagtacatcg agatcgccgt cttccatttt gatgttaaat
     6421 ccgcgacgct cccactcgtt caacacatag gacgggtaat agccgcgtag ctgcacatcg
     6481 gtaaagacgt agcgttcgcg catcgactcc tgagcgaaca tcacatcgtc cgggttacag
     6541 cagtagggat agagcggcac catcgccagc atacagccga ctttcatctc cgggttaatg
     6601 cgacgcgcag ctttcaccgc cagggcgctg gcgacaaact ggtgatgcag cacctgatac
     6661 atcgtctctt ccgggttctc atgctcggta taaaccacgc cggagcagca gtaaccgaac
     6721 agcggtgcac gccagttacg ctggttgtta atttcgttga aggtcatcca gtatttgact
     6781 ttatgcttat agcgttcaaa caccacttcc gcgaaacgga caaagaaatc aaccacttta
     6841 cggttggtcc agctaccgta ttgctgcacc agatgcagcg gcatttcaaa gtgggagagg
     6901 gtgatcaccg gttcgatgtt gtatttcagc agttcatcga acatatcatc gtagaacttc
     6961 agcccttctt cgtttggctg agcttcatcg ccttttggaa aaatgcgcgt ccaggctatg
     7021 gaagtccgaa aacatttgaa gcccatttcg gcaaatagct tgatgtcttc cttgtagtga
     7081 ccataaaaat caacggcttc atggtttgga tagtatttgc ctggcaagac ttctttggtg
     7141 atttcgcgcg gcacgccgtg tgcgccaccg gtcagaacgt cacaaatgct cggccctttg
     7201 ccgcctttgt tccagccgcc ttcgacctga tgagcggcaa ctgcgccgcc ccataagaaa
     7261 tctttctgta aggtgagttt tttcactatc ataggctcca gatgagtcaa ttcttacagg
     7321 aaatagtacc atgcagccaa acgacatcac tttttttcaa cgtttccagg atgacattct
     7381 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
     7441 tgtgcttcgt gtcgggcgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
     7501 aacctcaacc gtaacgctgg atacgctgac ggaaaaacat gcagagcagg aaaatatgac
     7561 cctgactgaa ctgataaagg tcattgccga catctatccc ggtcagacac agttttatgt
     7621 gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
     7681 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
     7741 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctccggagt
     7801 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
     7861 acagcgtcag tcacggcatt gggttggtgt ttggtatcgt tgggctggtg ttgctactgg
     7921 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg
     7981 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
     8041 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
     8101 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
     8161 tctggagcct ggcattgctg ggtattctgt ttaaactgac catcgcgcac cgattcaaaa
     8221 ttttatctct ggtgacctat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
     8281 tggcagttaa gctcgcggcg ggcagcgtta ccttattggc ggtaggcggc gtggtttatt
     8341 cgctcggagt gattttctat gtctgcaaac gcattcccta caatcatgcc atctggcacg
     8401 gcttcgtgct cggcggcagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
     8461 cgtaatgggt atgcctttgt aggcctgaaa agacgcgtca gcgtcgcatc aggcatcgga
     8521 gcacaactgc cggattcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt
     8581 gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat
     8641 gcctgatgcg ataaagagat ttactcttcg agcgaatacg gcagcggctc aatatgcaat
     8701 gtattcgcat cgtcgcgtac gcggaagatg ctatccggtt ccatatcgtt attcatgacg
     8761 acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga
     8821 cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctggcg
     8881 ctacctgcca gcaaccagag cgcacgttta ttggcaccac ggaattttgc tcgcgccacc
     8941 atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg
     9001 gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa acccgcttca
     9061 atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg
     9121 gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtattca
     9181 aaccatagca aagtggtcgc gccttcttta actacctgtt tttctttcga aggcagttca
     9241 ctaaagatat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacatgc
     9301 tcgtcgtctg gcgcgatggt cactttagag aacaccgcat attttttcag ttcagtcagc
     9361 tgcggttcac gcacgctgcg ccgttcaatc cacgcaaagc catcgccgtc gcggaacaga
     9421 cgtaaattgc tccacatttt gcctttggcg tcgcaatggg cggcgagcag gtgctgatct
     9481 tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg
     9541 ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca
     9601 gaagccgtag gctgacgggg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct
     9661 taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta
     9721 cgcgatctgt gccttctgcg aggcgacttc caaaaaaatg aagccattga attgttgtga
     9781 attaatttcg tgatcgcgat ggtgtttact gatattcccg taaaagaagg cgaaaaacac
     9841 gttacaatag cggggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa
     9901 agcccgcatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc
     9961 gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct
    10021 gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga
    10081 tgcagaactg gaaatgatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt
    10141 ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc
    10201 tggttgccgc tgttatttta ctcatgccct ggccactcag ttacaccccg ttatggatgg
    10261 tgttactttc gctggtggtg tttgattgcg ttcgcagcca gcggcgtatt aatgctcgcc
    10321 agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca
    10381 tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg
    10441 gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt
    10501 tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg
    10561 ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac
    10621 cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc
    10681 acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt
    10741 ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc
    10801 gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaacaatt
    10861 ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt
    10921 tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc
    10981 ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt
    11041 ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata
    11101 acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc
    11161 acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg
    11221 atctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa
    11281 atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg
    11341 ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg
    11401 agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc
    11461 agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga
    11521 aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg
    11581 cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg
    11641 ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc
    11701 agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtaccg ttaggtgaag
    11761 aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg
    11821 tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct
    11881 ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc
    11941 atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg
    12001 tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta
    12061 ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa
    12121 cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggtt
    12181 ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca
    12241 gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggcgtgt
    12301 tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagcggca
    12361 cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag
    12421 agatgatcgt ttataaagcg ctgcaggaaa aacacgtcat caccgtgttt actgatatta
    12481 cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca
    12541 ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga
    12601 aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa
    12661 gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc
    12721 ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc
    12781 agccgccgaa agagatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat
    12841 aattcgcgtg aaacaacaga tacaacttcg tcgccgtgca gtcgatgaaa cggcagactt
    12901 gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggcg tgcgcagtgc
    12961 gcaagaactg gaacgcagtg ttaaagggat gctgccctgg cagcaactga gcggcgtcga
    13021 aaaggccgtt gagatccttt acaacgcctt tcgcgaagga acgcggatta ttgtggtcgg
    13081 tgatttcgac gccgacggtg ccaccagcac ggctctaagc gtgctggcga tgcgctcgct
    13141 tggttgcagc aatatcgact acctggttcc aaaccgtttc gaagacggtt acggcttaag
    13201 cccggaagtg gtcgatcagg cccatgcccg tggtgcgcag ttaattgtca cggtggataa
    13261 cggtatttcc tcccatgcgg gggttgaaca cgctcgctcg ttgggcattc cggttattgt
    13321 taccgatcac catttgccgg gcgaaacatt acccgcggcg gaagcgatca ttaaccctaa
    13381 cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct
    13441 gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc
    13501 aattcctaac ctggcagaac tgctggatct ggtagcgctg gggacagtgg cggacgtcgt
    13561 accgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg
    13621 aaagtgccgt ccggggatta aagcgctgct tgaagtggca aaccgtgatg cacaaaaact
    13681 cgccgccagc gatttaggtt ttgcgctggg gccacgtctc aatgctgccg gacgactgga
    13741 cgatatgtcc gtcggtgtgg cgctcttgct gtgcgacaac atcggcgaag cgcgcgtgct
    13801 ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt
    13861 tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct
    13921 ggcaatgtat caccccgaat ggcatcaggg ggttgtcggt attctggctt cccgtattaa
    13981 agagcgtttt caccgtccgg ttatcgcctt tgcgcccgcg ggtgatggta cgctgaaagg
    14041 ttctggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact
    14101 ctaccctggc atgatgctga agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga
    14161 agcggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctgga
    14221 cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac
    14281 catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc
    14341 gctgtttgac ggtcatttcc ggctgctgca acagcggctg gtgggcgaac gtcatttgaa
    14401 agtcatggtc gaaccggtcg gcggcggtcc gctgctggat ggtattgctt ttaatgtcga
    14461 taccgccctc tggccggata acggcgtgcg cgaagtgcaa ctggcttaca agctcgatat
    14521 caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta
    14581 gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca
    14641 ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg
    14701 cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca
    14761 agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaatgaac
    14821 ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc
    14881 tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag
    14941 ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac
    15001 tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgctacc
    15061 tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc
    15121 gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg
    15181 aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg
    15241 gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taagagcccg tttgactccg
    15301 gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata
    15361 ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg
    15421 gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga
    15481 tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc
    15541 agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga
    15601 tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg
    15661 actcccgcat taaagatctg cgtaccgggg tagaaactcg caacacgcag gccgtgctgg
    15721 acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca
    15781 tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa
    15841 cgcgtcgtga gaagctggcg aacctgcgcg agcaggggat tgccttcccg aacgatttcc
    15901 gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac
    15961 tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg
    16021 gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc
    16081 gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca
    16141 tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta
    16201 ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc
    16261 aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc
    16321 gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc
    16381 gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc
    16441 gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg
    16501 aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta
    16561 acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct
    16621 acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc
    16681 tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg
    16741 gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg
    16801 acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc
    16861 acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag
    16921 aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg
    16981 cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc
    17041 gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc
    17101 tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt
    17161 acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc
    17221 gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga
    17281 tgcgtccggt aaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt
    17341 taattgtgct gcgcgaaagc agacaacaat tttcttgctt cactattggc tgcctgcatc
    17401 accatccacg gactgaacgc ccacggtgtg gcatcaatac cgtgtaatac atctgctaaa
    17461 tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt
    17521 gcggcaaata ccggacacac ttcattttcc acaatgccat tcggatcggt ggcgcgatag
    17581 cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa
    17641 cggcggatca ccgcgtcttc gttgctttct cccagttgtg ggtgcccaca aaccgagtta
    17701 gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgtcct
    17761 ttggcattaa acagccaact ggagaacgcg agatgtaagc gggtgtctgc cgtgtgtgcg
    17821 gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc
    17881 gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg
    17941 aaatgattaa ccaagtgagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg
    18001 taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa
    18061 cacagtcaga aatgacagga atagagagag taaaaagcaa cagcagactg aatataacgc
    18121 gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    18181 cacatacgta ctgtcctgac ttttaaatct ttatcagaga cggattcctt caccaggtca
    18241 gcatgatgct ggtagccatt aaagaagaca ttcagcacaa cggcgctaag ggttgcgagc
    18301 ataatgccgc tatgcagcaa cggttgcagt acggccggta acttagaaaa gaaatcgtga
    18361 gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag gttgtaacgg
    18421 ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac catgccgaac
    18481 atcaccagcc cagcaccgcc cagcacaaat tgcggaatgg aggcgaccag caccgccatt
    18541 tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac ccagcggcta
    18601 taaatccttt cgcatagcga gtaatgagca agataaaaat taagacagca aaggagatac
    18661 ctaaataaac gggattacca tattgcggat tccctttacc tccggcggcc cagtcaatac
    18721 ccacctgaat gatgctaagc ccaatagaag taataaccac accggtaacc agtggcggga
    18781 ataaaggcat caagcgacca ataaatggcg ctaataatgt ggtgataaaa cccgcggcga
    18841 tagtggcacc aaatatcccc agcaggccga tatccgggtt catcccaatg gctatcattg
    18901 gtgttacagc agcaaaggtc accgacataa tcaccggcag gcggatcccc ataaagcggc
    18961 cgataccgat acgttgcaat aatgtgacga tcccgcagca aaagagatcc gagctaatga
    19021 gcatcgcaat agcatctttt gagaggccca gtcggtcacc aatcataaga ggaacagcga
    19081 ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag tatgatcagc tttcctggtg
    19141 ataatatgcg gtcaacctca tcagttgggc ggtcttgccc agaagatgag ggaagttggg
    19201 aatctatggc gctcatcagt gtttctcctt tcgcccgcca gagtaaaaag caaccgttta
    19261 cgttaatgaa actgtctaaa cacatgctcg tggagtgcca gtttttgcga ggaaggggag
    19321 ttagagacag gaattgcgga ggcaatcaca taatcgtgct taaaccacga aaatgacaag
    19381 agaacctgac aatggaatta tctgtaattt tctcatattg atatttcatt aatcattctg
    19441 gcatgtcgca ctctagcatt taatcgtttt tatctggata gcgctctttt gatcggcaat
    19501 gttgaaataa tgaaagcggc atagattgag gggcaataat ttgaactcag tcgcaaaatt
    19561 ataattaaaa cggtcatgct acctaatata tgcccgttta ttgcatcgcg aaatttgaga
    19621 agacataact cattaaatga aagggatgca aatgaataag tttatcgctg ctgaagctgc
    19681 ggaatgtata ggctgtcatg cttgtgaaat tgcctgtgcg gtggcacaca atcaagaaaa
    19741 ctggccgctg agtcacagtg actttcgacc gcgtatccac gttgtaggga aaggccaggc
    19801 tgcgaatccg gtggcctgcc atcactgcaa caatgcccct tgtgttacgg cttgtccggt
    19861 taatgctctg actttccagt ccgatagcgt acaactggac gagcaaaaat gtattggctg
    19921 taaaagatgc gcaatcgctt gcccctttgg cgtcgttgag atggtcgata cgattgcgca
    19981 gaaatgcgac ctttgtaacc agcgcagttc cggcacgcaa gcctgtattg aagtctgccc
    20041 aacacaggcg ttacgactga tggacgataa agggttacag tggataaagg tggctcgcca
    20101 gcgcaaaacg gcagcaggaa aagcgtcatc agacgctcag ccatctcgca gtgcagcgtt
    20161 gctccccgtt aactcgcgta aaggcgcaga taaaatttca gcgagtgaac ggaaaaccca
    20221 ctttggcgaa atctattgcg ggctggatcc actgcaagcg acttatgaga gtgaccgctg
    20281 tgtttattgt gccgaaaaag ctaactgcaa ctggcattgc ccgctgcata acgctattcc
    20341 ggattacatc cgtctggtac aggaaggaaa gattattgaa gcggcagaac tttgccacca
    20401 gaccagttcc ttaccagaaa tctgcggcag ggtatgccca caggaccgtc tttgtgaagg
    20461 cgcatgtact ttgaaagatc actctggcgc agtctctatc ggtaatctgg aacgctacat
    20521 caccgatacc gcgctggcga tgggctggcg tcccgatgtc agcaaagttg ttccccgtat
    20581 cgaaaaagtg gcggtgattg gcgctgggcc agcagggtta gggtgtgctg atattctggc
    20641 gcgcgcggga gttcaggttg atgtctttga tcgccatcca gaaattggcg gtatgctgac
    20701 ttttggcatt cctcctttca aactcgataa aacggtatta agccagcggc gagagatatt
    20761 caccgcaatg ggaatcgact ttcatcttaa ctgtgaaatt ggccgcgata ttacctttag
    20821 cgatttaact tctgaatatg atgcagtttt catcggcgtg gggacttacg ggatgatgcg
    20881 agcagatctg ccgcatgaag atgcgcccgg tgtcattcag gctctaccgt tcctgactgc
    20941 ccatacccgc cagctcatgg gattgccgga gtctgaagag tatccgctga cggacgtgga
    21001 aggtaagcga gtcgtggtat tgggcggtgg cgatacgaca atggattgtt tgcggacttc
    21061 cattcgcctc aatgctgcca gcgtgacctg cgcgtatcgt cgtgatgaag tcagtatgcc
    21121 gggctcgcgc aaagaggtgg ttaatgcgcg cgaggaaggt gtcgagtttc agttcaatgt
    21181 tcaaccgcaa tatatcgctt gtgacgaaga tgggcgttta actgcggtgg gcctgattcg
    21241 taccgccatg ggtgagccgg ggccggatgg tcgccgtcgt cctcgtccgg tagcgggttc
    21301 agagtttgaa ttgcccgccg atgttctgat tatggccttt ggtttccagg cgcatgccat
    21361 gccgtggttg cagggcagcg gaattaaact cgataaatgg ggcctgattc aaaccggtga
    21421 cgtcgggtat ttacctaccc agacgcatct gaaaaaagtc tttgctggtg gtgatgcagt
    21481 tcatggcgcg gatctggttg tcactgcaat ggccgcagga aggcaggcgg cgcgcgatat
    21541 gttaactctg tttgatacga aggcatcgtg atgaaatcgt taattatcgt taatccggct
    21601 gactgtattg gctgccgtac ttgtgaagta gcctgtgtgg tcgctcaccc ttcagaacag
    21661 gagttgaatg ccgatgtctt tttaccccgg ctgaaggtac agcgactgga cagcatcagt
    21721 gcgccggtga tgtgccatca gtgtgaaaac gccccttgtg ttggcgcttg ccccgtgggg
    21781 gcgctgacga tgggcgagca ggtggtgcag accaattctg cccgttgtat tggctgtcag
    21841 agttgcgtca gtgcgtgccc gtttggaatg atcaccattc agtcgttgcc gggggatacc
    21901 cgacaacaaa ttgtgaaatg cgatctctgt gaacagcggg aagaggggcc agcctgtgtt
    21961 gaatcttgcc cgacacaggc gctgcagttg cttactgaaa gagaactcag gcgagtccgc
    22021 cagcagcgta ttgttgccag cggtgagaat cctctgtgaa agaaatctcc gcgctgaaag
    22081 gcgcggagaa tctattagat tgcccaacca cccgcgtaaa atgcgaccag tgcgacagga
    22141 acgctaattt gttgcccgag taccagactg aatgcggtaa atgccgtcaa ggaaatcgca
    22201 caaccaatcg ccattggcaa attggcctac aaccccatca gcaacgagcc gaagcccgcg
    22261 accagacagg tggcaacaaa cacagctgcg ggaggaaaac ctgctttgcc cagcattccc
    22321 ggaacgacga taacggaata aaccatggcc agaaaggtcg ttaagccagc cagtacttcc
    22381 tgacgaacgg tactgccacg agcggtaatt ttaaaataat tatccagcgc gccctgtggc
    22441 tttggtgcgt ccggtgtttg taggatgtct ccagacataa tgatgtcctc acgaggatgt
    22501 taaagtttta ttattagttg cgttcgtaca ccagacgacc atcaacgtag gtgcggtaga
    22561 tcgaacggtc atcgcccaac gtcatcatca cgaacaattt gtcgactaaa gaaacagagt
    22621 tgtcatagcg cagctgctgt agcggagtgg cggtgggttc catcaccacg aaatcagcct
    22681 ctttgccagg taaaaagttg ccaatcaaat cgtcaaggcc cagagatttc gctccgccga
    22741 gcgtggccag gtaaaacgct tcatatgccg agaggcgata gccttgtaat tgcaacactt
    22801 tgtaggcttc gttcagcgtt tgcagcatgt tgaaagttgt tccggcaccg atatccgttc
    22861 ccatgcccac tttgactttc ttctgcccag atgccgcatg catcgtgaat tgcggtagca
    22921 atcgccggag ctgcgccatt tacaccgatt tccgagatcg atttcgcccc gaacgggccg
    22981 actttatcgt cgctcggcac cagcacagcg cggaaatcac gcggaatgtc accaattttc
    23041 ggtgcgccgt aactgcgtaa atcacgcgtt aacgggtgac cttcggcgtc ataaatgatc
    23101 tcttcgctca tactgtggcc gatagctcgc agggtagcac cgtagatttg tcccaatgct
    23161 aattccgggt tgaccggtgt accgcagtcc agcaaggcgt agaatttatc caggcggatt
    23221 tcacccgtac gcgtgttgac ggcaacttca gcgaagtttg cgccatacgg gaaggcgaaa
    23281 tcaggcgtga tataactgcc agttcccacc agtgaaccaa agccgatgcc ggtttcgcct
    23341 ttatgggcaa tatccccgaa actaacttcg cctttcttgc cgcgcacgac gcccggcgtt
    23401 gccagttgaa catctgccac tgcctcacct aacatttgcg cgccgtggaa cagaattttc
    23461 tcccgtagat tttccgctgc caaacgcgcc gcgttacccg agaagcaagt accggacgag
    23521 gcatatgcgc ctttatcaaa caacgcatga tcggtatcac cggagataac atgcacgtcc
    23581 tgcggtgggc agtgcagcac ttctgctgcc agtttcgtca ctacagtatc cagaccagta
    23641 ccaatatccg caccgccaga atgaacgata aaggtgccgt ccgattccag tttgatcatg
    23701 cagttagcct gatcgatatc cgggataccc gatttctgca tgataatggc gacgccgcga
    23761 ccgatgtgcc agtcaccatt ttgcggtttc ggtgacgacc attggatcat ctcgcgaccc
    23821 tgacgcagga tctcttccag tgcacagctg gcggcggaag gaacggaggt cggcgcttta
    23881 ccttcaccga ttgcaccgag aattttcagc tcttgccctt cgtgtacccg gttacgttcg
    23941 ataatttcca gttggtcgat ctgtaactgt tcagccagtt ccgctaatgc catggtgata
    24001 gcgaagttac ctttcggtgc gccataaccc tgataagcac catttgggca aatgttgctg
    24061 tagtaggtgg tgacctggaa atcgacgtta tcgcacggat ataacggcag cgacagcgcc
    24121 ggtccgttac acggtacggt gagtgagtgg ttgccgtaag ggccagtgtt ggcgcggaaa
    24181 tccatcttca ctgccgtcag gcgaccatct tttttcgctc ccagtttgac ggtgactttc
    24241 gcgacgtgac gagaggtgtt agcaataaac tcttcttcac gggtgtagcg gaatagtacc
    24301 ggacgcccgg tcacgcaggt tgcccaggcg cacacttctt ccagcaggat gtcctgtttg
    24361 gaaccaaaac cgccgccaac tcgctcttta atgacatgaa ctttatgctg tttcatgccc
    24421 acgaggcgcg cgacctggcg gcgtaagtgc catggtacct gggtggaggc gtggataacc
    24481 agacgatcgc cgtccatccg ggtaaagcag atatgtgttt cagtcgggca ctgctgtgcc
    24541 tgcgttgagt tataggttcg ctcaatgatc acatcggcat cggcaaagcc tttgtccata
    24601 tcgccaatat gaccatgaat actggcggcg atatttttgc gagggcgaga accgatcggg
    24661 aagttgatga tcatatgctc gccgcgctgg gctgcatggc tgttatcgtc ttccagagta
    24721 tctggcgcac cagcaacata caccaccggt tcatcgtgca cgacaggcgc atcttccgcc
    24781 attgcttcgt cgatcgacat taccggctta agcacttcat attcaacgtc gatgagcttc
    24841 agtgcttcga gcgcaatttc ttcactttct gcgacgaccg cagcaacacg atcgccgacg
    24901 tgacgcattt tcttgccgaa catacggcgg tcaagcggtg acggttccgg tgcgctctga
    24961 ccccccgggg tatagtagat atcagggcaa ttcaggtgag taataacgtg aacgacgccc
    25021 ggtaaggctt cagctttgct gacatccaga tgagtaatca gtgcgtgagc gtgtgggcta
    25081 cgtaacattt taatgacgca ggcgtcagcc gttacgcagt cttcaacata gcagggtttc
    25141 gcctgcacca ttttcgcggc atcagtttta ggataatgct tgccaatgac gtcaaggtcg
    25201 tcacggaaag tcggagcgat atcaatggtg gcctgcggat tatttttacg tgcaaccgcc
    25261 agttcaatga cctgatagta ttgttgccag cctgcatcgc ggctgaacaa accagaaagc
    25321 gcgtcgtcga tctcttcgcg ggtaggtgcg gcgatgcgat cgagaagatc ggtgataatc
    25381 agagctgcag ctggatcgtt ataaccagac tgcaccacgc caacatcaac cattgcctgt
    25441 tgaaccagac ttaactcgtt ccatttaccc agagattctg cggtacgaat atctgccttc
    25501 tctaactgtg cggcaataag caaggacgcg ttaacgatat taccgttaaa gattattgcg
    25561 tcagaaccgg caaacccgaa gccatcatca ctgttgcgta cagagtgcat tcccatgtta
    25621 aacaacagct tttgcacgtt ttcgcctgga ttaacggtta gctcctgagg cgcgccattt
    25681 aaagtaaagt ggatgatcat acggcttcct ccccagcctg ttggcagtcg gcatacaggt
    25741 cggctaccag aactcccgtg atatagcgtt tataggccac gctgccccgc aaatcttcct
    25801 gcgggaaaat ggcgttggcg acagcctgtt caagtgcatt gccttccaga ttttgttttt
    25861 cgacatcgtg cagacgcagt gctttactgg cgacgccatc cagcgcaatt cgcataccgt
    25921 cgtggtctgt cattgcaacg gctgccgtca cgacggttaa acctgcctga gaacggctaa
    25981 ttttgcgggt cgcacaggtg cgatacggat ctttaatgat aatttcggtt agcaggcgat
    26041 cgcatgggca ggccaggtag tcctcgattg acagcgtttc gccgttgcca aaaaccagtt
    26101 cagcatccag tgctagcagg acgggaagca gcactgactc ttcctgccgg gcggcgattt
    26161 caccaccaat ggtcgactga ttacgaacat ggcgtgagta aacaaaaccg agggcttcac
    26221 gcagtgctgc aggaataaag cgcgcatcac gcagtggctg caagcgagac attgcaccaa
    26281 tccgcagtgc accgttatcc cagtcaaccc agtccagttc cagatcctgt aaggaaatgg
    26341 caatcttttt atcggtacgg gttggtgtag cattgagttt gctgcccccg gcgaaccaga
    26401 cggcttcatc ctggtagcgg cgcttcagtt ccagcgcctg ttcgacagag tcgggcctga
    26461 aaaattgttc aatcatcttc tttccttatt taaccctctt ccctgcggga agagggcatc
    26521 tgtcatttat gccagcgcat ccatccgacg ccacatactg gcagcggctt ttctggcttg
    26581 tgcataaatg gaatcgcaat cgaagttaaa ctgacggtct tcatagacca tcacaccatt
    26641 gaccatcacg ctgtgaacgc tgcctgagcc cataccgaaa gcgatatgcc cggcgatatt
    26701 gtctgccagc agcggcgtcg gcgagttgta atcgcaaatg gtcaaatcag ctttgtaacc
    26761 ggcttccaga agcccaaatt tcgcgccaaa gttgcggctc atcagttcgt taccgttagt
    26821 cagggctttg gcaaaactgt caggccacag cggaccaccc gcatcgcgat gtttaaagaa
    26881 ggcaaatttc atctcttcaa acatgtccga accaatgccg tccgttccca atgccaggtt
    26941 gcggatgtcg ctaaggtgat ggttgtagcc gacatggttg ttcatgtttg aacgggcgtt
    27001 atgcaccagg aacgcatcgc gctgattgag tagggcgatg tcatctttcg acaagtacag
    27061 cccatgagcg accagcgttt tgctgtcgat gaggtcgaat tgcgccagtc gtgccagcag
    27121 gtctttgccg taccagtggt gactgtagga aacgtcgtaa aggtcttccg cagcgtgaat
    27181 atgcaaacca cggcctgtgg ctttcacggc ttcacgcagc atctccagac cggcatccgg
    27241 cacggtaaac ggcgcgtgag caccgatatg tgcttccacc agatacggct cgcttgtcgc
    27301 tttcttcgcc tcatcaatca aacgggcgaa acggatgttt tcttctacac cttcctgcaa
    27361 ctctttgatg ccgttgttac ggtcagtagt ttcaaaacag gtcatcgcgc gcaggccaac
    27421 ttttaaaaat gcgtcgcgca atgtggagag cgacccgccg atatacgccg gcgaggcatg
    27481 gtgatcgata accgatgtac atccgctctt aatcgcttcc agggaacaaa tcagtccgct
    27541 gtaatagagc gactcttcat caagggcgcg atcgagccgc caccagagat ttttcagcgt
    27601 tgagatgaaa tccgggcaag gggcgatgtt tgccataatt ccgcgggaaa gctccgagta
    27661 aaaatggttg tgcgagcaga caattcccgg catcacaatc cgaccatgca tctctttgta
    27721 gctggcatcg gggtagcgtt gcgtcagggc atcgccgata gcgacaatca cgtcattttc
    27781 gatagcgata tcaacgcctt cctgcacttt cgccgggtgt aactgcactg cggtgacatt
    27841 cttcagaatc aacatgatta cacctccacg cggcccagca gataatgatg atgctggtgg
    27901 acatggctga tgatgcggca catatcgttc agctccggtg gtacgttgtt aaactgacct
    27961 tcgctgtcga tgtttaacac ccagctttgg ttattcagac gtactcgtac ccggcaatct
    28021 tccacaagga agcctggggt gctgctgtta tcaaagtctt gcgccaggct gaagacggtg
    28081 attttgtctt cgtatggttt accgttccac ggacagaact gagcgcagtt gccgcattcg
    28141 ttacagtaag cgtcgaggtg cagcgtctgg aatctattct ggaagccagg gactgcaatg
    28201 gagacgttgg cgcggttcgg gcagacatcc acacacttgt tgcaaacgta gttacattcg
    28261 aggcagcgag cggcttcctg ggcgacaaac gcgtcacgat cgtcactgtt caccagcgtg
    28321 atagagatat cgcctttacg ttgatagatt tccgctggat tgacgttgtt ccagtattta
    28381 tcgttctggt gggaacggat attttcccga ctaaggatgg catcggtcgc ccgacgcgcg
    28441 gttccgacag cagcgacaat ggaggatggt ccgcgctgta cgtcgccgat cataaagacg
    28501 tcagtcagac gagtttcgcc attatggtcg acgtctggcc agccgttttt gtccagcggc
    28561 acgcccatcg cattcagggc ttcagtatcc tgctgttcac caatggcggt gatcaggctg
    28621 tctacatgca gtgttactgt ttcgttggtt tcaaccggac ggcgacgacc tttctcatcc
    28681 ggttcgccaa gcgacataac gcgcaaggtt aaggtgccat cagcatcgaa acgttccgga
    28741 ttattcagga aacggaactc tacgccgtcg tgcaacgctt cttcatactc ttcgcgccat
    28801 gcgggcatct cttgtagtga acgacggtaa acgatcgttg ctttttctac gcctggaacg
    28861 cgtaacgccg cacgagcgca gtccattgcg gtgttacccg ccccgacaac gaccacatgt
    28921 ttgcccagct tgagcgctgt acccttgttg tattcacgca ggaaggggag tgatttccag
    28981 acattttggt tgtcgcccgc cagtttcaca ccgctatttt tatcagtgcc ggtggcaatc
    29041 agaacatagt ggaagccctg attttttaac tgttcaacgg ttaaatcggg tgagcagcca
    29101 tactcaaatt tcacgccgtg agcggcaaca aaatcgatat cgtgctgaat taactctgca
    29161 ggaatacgga actgaggaat gatatttttc accacgccgc cagcattggc ttcgcgttca
    29221 aacagcgtaa ccggatggcc cgctctggca aggaagtaac ctgctgccag acccgccgga
    29281 cctgcaccaa tcacggcaac cggatggcgt gaaccagaac cggcgggttt gtgccagcgt
    29341 tgcttatatt catcccaacc tttttccagc gcgacttttt tcagttcgcg gatattcagc
    29401 gcactgtcgt aatccaggcg ggtacagttg tattggcact ggtgatcgca aatatgaccg
    29461 gtaatggcag gtagggcgtt gcgttggtag atgagttcca gcgcgtcggc atagcggtgt
    29521 tcgccaagca gacggatgta ttccggaata tcttgcttga tagcgcaggc agtaacacag
    29581 ggggcaacgt agcaatcggt cagcggcagg tcttctgcca cttcaatacg ctcttctggc
    29641 ttccagtgtt tctgggtgta ttccatggtt aacgcgtctg ctgccagtct gttcagtcgt
    29701 tcgacgtcaa catggtcaag tccccaggcg tcggagcctt ccagctcgcg catgcaggca
    29761 cttaagcgca gatagccgcc aggtttcagc aggtcggttg ccatagtaat agggcgaata
    29821 cctgtatcaa aaatatcgcg gatagtcagc tgactggcac caccggaata agaaatgggc
    29881 agtttgccgt caaaggcgcg agagagaact gctgcaacat tgatggagag cgggaacagc
    29941 gcacggcctg acatatacat ctcttcacca ggcagtgcgc ctttattgtt gatggtgccg
    30001 agagtgttag tcagttttac gccaaagccg agtgattttt cttttgccag taccatcagg
    30061 cgctccagca tttccagtgc ttgcgtcagc ttgaggtcgt gatcaaatga ctcttctttt
    30121 aagcctatgt aaccgaaacc gcagacatcg aggatctcac gaacacgcgc gtaccccagt
    30181 aaggtcgggt taagtttgac aaaggtgttg agcccttttt cttccagcat gtagcggcaa
    30241 atggcttcga tttcatgcgg aggacagccg tgcatggtgg agagggtgac gccctgcacc
    30301 atactggtgg ggatgcgagc gggtaaggct tgcaagtttt cgcgtttttc ctgcaatccg
    30361 tggcgggcta aaaatgcgtc atcctggagt aatttattca gcgtatcgcg atattgagcg
    30421 aatttcggat ggtcagatgc gtccatcata ttgtcgatga actgttgcat cggcggttgc
    30481 ttaatacctt cgaggttgta accgacgctc atattaaaga tgaacgattt accggaatca
    30541 gaaggctgta acatcgcttc gagaaggtgc agggcaaacc aggctttgag gtattcatcc
    30601 caggctttaa gcaaggtaaa ttcggtagac cattcggtgt taaagcattc gtcttcggca
    30661 tcaatacagg gcttttccag ctccaggcgg tcaagaattt ggacggtttt tagttcgatg
    30721 aagcgtccgc cagtcagcca ggaagtgaca atattttgcg cgagctgcgt gtgcgggcca
    30781 gcggcagggc cgacgggagt ggcacaggtt tcaccgaaga cgctaacagt tttacctttt
    30841 acaggtgagt aaaactgttg ctcgggaata ccaaagattg agcgttgttg ttggtattca
    30901 tcaaatatgc gcgtcaaaag ttcctcaaac ggaatgggac gcataatatc ccccataacc
    30961 ctctccttag cttatacaga tatcattgag tgaccggtgt tgagaaatcc ggtggtttag
    31021 ggggatagag caacaatcgc accactcttg ccaggtttgg cgggaaatat aaaaaaatgt
    31081 gaggtggctc gaaacttaaa ggctttttgt gaatgaactc acaggtgcca aatgagaatt
    31141 aaaatcactc ctgaaaatta ttattgtcag cccatttatc actttttctc ttttctcaaa
    31201 atcagtgatt ccttatcatt atgataaaga gtgctggaga gattaaatat ttgaacgcaa
    31261 tcgtttaact acattttcta aattttagta tacctttttt gcgctgtaat aaagtcgtcc
    31321 ggagtatcaa tatctaaaat tatttctgca ttttctattt cgacggaata atgatctccc
    31381 ataaggagtg cctgacgcat attggtgaca ttgggtcgct ggattgcctg catcaggcat
    31441 ggttttgaca ctaaaatcgg atggccgggg atgccattat ggagtggcag tattgcgcca
    31501 tcatttcgta acgaccagat ttttctaaaa atatcgatgg tgagggttgg catgtcaccg
    31561 tgggtgagaa aacaatgttc tgtttgcact gcgggtactg cggccttgac ggatgttagt
    31621 aaaccctgcg cataatctgg gttgtgaata atagtgatat tgctctggtt cgcataacgt
    31681 tcgtgcagtt cattaccacg atagccggtg actaaaataa ttcggctaca aaactgcaac
    31741 gcatttttga tacttgtatc aagaattgtt ccctgttccc aggggaacat cattttccat
    31801 tgccccattc ttgatgataa tccggcagcg gtaattatac agtcgatggc tgacatagtg
    31861 gttccattat ttataggggc taataacgcc tgataattgc gttacttcct gatggattaa
    31921 gttggggata atttatccca gagaggtcat aaagactcgt gaaaagtata gttgacccat
    31981 cggcattagt cattgattta ggtgcgcaga aacgtcccac tgtgatttct gttgttggtg
    32041 ctggggggaa aaccagcttg cttttttggc tggcagaact gctccaggct agcggcaggc
    32101 gtgtgttaat cacgacgacg acacatatgt ttatgccaac gtctcactgg cccgtggttt
    32161 tctgtcgtga tcccgccatg cttcctcatg cgtctcttac atcccccatt tcattttgtt
    32221 ttcacagctg gaaagcgaac cagggaaaag tgcagggatt tacgccagaa gcgattgatg
    32281 cactggtgca acgaccagag tgtgacgtaa ttctcattga agcagatggc tcgcgtggaa
    32341 tgccgttaaa agcgcctgat gagcacgaac cttgcatacc caaaagcagt tgctgcgtga
    32401 ttgctgtgat gggaggacat actttgggcg cgaaagtgag cacagaaaat gtccatcgct
    32461 ggtcgcagtt tgctgatatt accgggttaa cacctgatgc aaccttgcaa ctgagcgatc
    32521 tcgttgcgct ggttcgccac cctcaggggg cgtttaaaaa cgtaccgcaa ggttgtcggc
    32581 gagtctggtt cattaaccgt ttttctcaat gtgagaatgc gattgcacaa agcgagctcc
    32641 ttcaaccgct gcaacaacac gacgtagagg caatctggct gggcgatata caagaacatc
    32701 ctgcaatcgc gcgcagattt gtgaattagc ggcccgggta ttccgggtta ctgaaaggat
    32761 atccattgag gttggtatga atattttcac agaggctgca aaactcgaag agcaaaattg
    32821 tccgtttgcg atggcgcaaa ttgttgatag ccgaggctcg actccccgcc attctgcaca
    32881 aatgttagtg cgcgccgatg gttctatcgt cggtacaatt ggtggcggaa tggttgagtg
    32941 gaaggtgatt gaagagtcac ttcaggcatt gcaggaacgt aagccgcgat tattccatgg
    33001 acgtatggct cgtaacggtg cggatgctgt cgggtcagat tgtggaggtg caatgtcagt
    33061 atttatcagc gtccatggta tgcgtccacg tctggtgttg attggcgcgg ggcatgtcaa
    33121 ccgggcgata gcccagagtg cggcgctatt aggatttgat attgccgttg ccgatattta
    33181 tcgcgaaagc ctcaatcctg aactattccc accatcaacc acgcttctcc atgctgagtc
    33241 gtttggtgcg gcagtggaag aactggatat tcgccctgat aattttgtcc tgattgccac
    33301 gaataatcag gatcgtgaag ccctcgacaa actcattgaa cagcccattg catggttggg
    33361 gttgctggca agtcgccgca aggttcagct tttcctgcgt caattgcgtg agaaaggcgt
    33421 ggctgaagaa catattgccc gtttacatgc gcccgttggt tacaacatag gtgcggaaac
    33481 gccgcaggag atcgccatca gcgtgctggc agaaatatta cgggtgaaaa ataacgcgcc
    33541 gggtgggctg atgatgaaac cttctcatcc ttccggacac cagctggtgg tgattcgcgg
    33601 tgcgggagat atcgccagtg gtgtggcgct acgtctgtat catgcgggtt ttaaagtgat
    33661 catgctggaa gtggaaaaac cgacagtgat tcgttgtacc gtggcgtttg cccaggccgt
    33721 gttcgatggc gaaatgacgg tcgaaggcgt cactgctcgc ctggcaacca gctctgcgga
    33781 agcgatgaaa cttaccgaac gcggattcat ccctgtgatg gtagatcccg cctgttcatt
    33841 gcttgatgaa ctgaaaccgc tttgcgtggt ggacgctatt ctggcgaaac agaatttggg
    33901 aacgcgggca gatatggcac cagtaacaat cgcgcttggg ccgggcttta ctgcagggaa
    33961 ggattgtcat gcggtaattg aaacaaatcg cgggcactgg ctcggtcagg tgatttactc
    34021 tggttgtgcg caggagaata ccgatgttcc tggcaatatt atggggcata ccacccgacg
    34081 ggtgatccgt gctcctgctg caggcattat gcgatccaac gtgaaattag gcgatctggt
    34141 gaaagagggc gatgtgattg cctggattgg tgagcatgaa attaaagcac cgttgacggg
    34201 gatggtgcgt ggcttgttga acgacggcct ggcagtggtt ggtggtttta aaattggtga
    34261 tatcgatcct cgtggtgaaa ccgctgattt caccagcgtt tctgataaag cccgggcgat
    34321 tggcggcggc gtacttgagg cgttaatgat gttgatgcat cagggcgtga aagcgacaaa
    34381 agaagtgctg gaagtggctt aaatgaaaat ggccggatgg tattacccac ccggccttaa
    34441 aattactgac aaatcaccgt tccggttttg ccttcaatcc cttctttcgc tttgctcaat
    34501 acggtaatca gtgcttcacg acctgcgcga gagcgagcaa acgaggcggc agcttccact
    34561 ttaggcagca tagaaccttt cgcaaaatga ccttcttcga taaagcgttc agcatcactt
    34621 aatgacaggc gatcgagcca ctgttcattc tctttaccga agttaatggc cactttttct
    34681 accgcagtca gaatgatcag catatcggca tcaatcattt ctgctaaacg ggcgctggcc
    34741 cagtctttat cgataaccgc gctggcacca cgcagatggt taccttcacg aataacaggg
    34801 ataccaccac cgccaacggc gatcaccacc tggcctgcat ctaccagagc tttaaccgtt
    34861 tctttttcaa tgatatcaac cggttttggc gaggcaacaa cacggcgata gccgcgacca
    34921 gcatcttctt tcagagtgta accctgtttt gtcagttgct ctgcttcttg ctcggtaaag
    34981 aacgaaccga tcggcttggt cgggttgagg aatgccggat cgttagcgtc gacttcgacc
    35041 tgagtaacca gcgttgctac aggtttatta atgccccgag aaagcagttc ttctcttaac
    35101 gcgttttgta gatcgtaacc aatataaccc tggcttaagg caacacagac agacatcggc
    35161 agcattggcg agtgtgcttc agtttttgcg gcagcttcaa aggcttggtt aatcatgccc
    35221 acctgcggcc cgttaccatg agtgacgata acttcatgtc cttgggcaat taaatcaaca
    35281 attgcctgag aagtaatttt taccgctttc atttgtccgg ccaggtcatc gcccagcgca
    35341 ttcccgccca gggcgagaac aattttctta ctcatttttc ctcactcatc attcttgatt
    35401 gtgtatagat ccctgacagt ggttagagca cgggagggac aaacggtttg cgtcgcagga
    35461 aacgacctcg cccggctttg cctgtaaagg tgccgtcaca gaaaatcgtt tcaccacggg
    35521 ataaggttct gacaatcgcg ccctgacagg taaaaccctc ccatggcgag tagtcggcgt
    35581 tgtcgtggag atggcgatgc tgaatttgtt ggctctgacg tgggtcgata atcaccacgt
    35641 cgccatcgga accgggcgct aataatcctt tttgcggcca caggccaaac agcctggcgg
    35701 gcattgcgct ggttaattca acaaagcgtt ccggtgttat acgtcccgtc atcacgccac
    35761 tggaaaacag taactgcatg cggttttcca caccgggtaa gccatttggg cagcgactga
    35821 aatcgccttt agaaatttgc aggcgttgag ccatcgagaa ggtgcagtga tcggtcgcca
    35881 ccacgtcaat cgcaccatcg ctgatgccac accacagttt gtcctgctcg cgtacgttac
    35941 gcagcggtgg gctaagaatg aacttcatgc catcttctgt atcgtaactg cgttcgtcca
    36001 acaggagata ttgtgggcag gtttcaaccc agactggctg gtgattcgca cgggcaagac
    36061 gcagataatc cagacctaag ccgttagaca ggtgcacgat atagagcggg gcgttaccgg
    36121 caatttgtgc caggttaatc atgcgggcga tggcttccgc ttcgcattcc agagggcgac
    36181 tcaaggcatg atagcgcggc gcggttaacc ctgcggcgat aaattccgcc cgcttgctgg
    36241 cgatagccgc atcattttcc gggtgcacgg tggtcagcgc gccggattca tgcaagcggc
    36301 gtaatgcctg caaaacctcg tcatcgttga gtttgtattg ataggttaaa tagagtttaa
    36361 aactgctcag tccttcctcg accatcatcg ggatttcgtc gaggattgcg tgattaatgt
    36421 gctggatcac accgtgaaag ctgtaatcga tgaccgcttt atgggcggca taaccacgat
    36481 aaacctccag ttgatggcgt aaccgacagc cgtttgggcc aaatcccata tggtcaataa
    36541 tggttgttgt accgccacac gcagctgcgc gggtaccggt aaaaaaatca tcacaactgc
    36601 gcgcgatgcc gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt
    36661 aacagccagt ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact
    36721 ggcgaacaat cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga
    36781 cagtgccgtt tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga
    36841 ggggaataag tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac
    36901 atcgcggcac aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca
    36961 ggttctttac ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg
    37021 ccgttagttg agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag
    37081 gcattcacca gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag
    37141 cattcggttg ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca
    37201 accgcgttag ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag
    37261 gtttcgcccc aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa
    37321 cggcttgggg atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg
    37381 tcataaccca gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa
    37441 atggcgttgt caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata
    37501 cgaatttcca tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg
    37561 accacaaatt ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg
    37621 cagtcttctt cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg
    37681 tctttaatga ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta
    37741 ccgcgaccac cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt
    37801 ttgatgttgc caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg
    37861 tggccgatat aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact
    37921 ttttccatct cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga
    37981 atagcaacca tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca
    38041 agaatcagtt tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg
    38101 ttttgcaggg agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag
    38161 acaacttcgc ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg
    38221 gcatctttgt ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc
    38281 gctgcgagaa cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg
    38341 ccgtacggat tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg
    38401 aattgggtgg cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc
    38461 aggcccgcca tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg
    38521 gagcgataaa tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg
    38581 ctgtagacgt cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc
    38641 agcagaacgt gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc
    38701 agggttgcgt agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg
    38761 tcctgtacca cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg
    38821 tcatagttca tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt
    38881 tcctgagcag aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc
    38941 cacgccacac cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg
    39001 atggcatttt tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac
    39061 aataattgag cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt
    39121 tttgattcgt ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc
    39181 gcacaaagcg gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg
    39241 gcctggcttt gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata
    39301 tcaatcttca atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc
    39361 gaccagcaga tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc
    39421 tcgagcagtg cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa
    39481 ggcttccagc tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc
    39541 acttcacctt ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg
    39601 gcttcgccat cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc
    39661 gcgttctgtg ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc
    39721 agcaattcag tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg
    39781 tctgcgtctt tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct
    39841 ttagcattgt ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg
    39901 gccaaggtga catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag
    39961 agcggtttgc catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg
    40021 ttttccagtg aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg
    40081 tggtcaatat cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg
    40141 taaccgtcat caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac
    40201 atatcgtcgc gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc
    40261 acggtttcgc cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg
    40321 agcaggttaa gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg
    40381 gaaataccta atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt
    40441 aatgctgcgg caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac
    40501 aaaaaatctt tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta
    40561 acagttttca tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt
    40621 gacttgccaa agtgattggt gttttcatag ctatacctgg gactaacaat aagtgtgcca
    40681 gaattatccg cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa
    40741 attgatgcca gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt
    40801 tatttatttg ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt
    40861 tgataaactc accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat
    40921 tgcattagaa aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat
    40981 atcatcatga tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa
    41041 ggtaaagcta tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt
    41101 cagcgttttg ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa
    41161 aacttgtgtc gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc
    41221 ggcaactcac gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag
    41281 tctcgcttcg atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca
    41341 tttctgggta cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc
    41401 gttacccacg agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt
    41461 cgccatatat ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac
    41521 atcagtaaaa tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat
    41581 gatgaaagtc gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa
    41641 aataatatta ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact
    41701 catggacata tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa
    41761 ttgcataaca ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt
    41821 ttttctgtag caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt
    41881 gttatgcggc aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg
    41941 gtggttggtg aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc
    42001 ggaagacgga ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg
    42061 gaaagcgaac tgttcggtta tgttaaaggc gcatttacta gtgcttctgc caacggtaaa
    42121 acagggttga ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg
    42181 ccattaatgt tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt
    42241 ggtgccagta gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg
    42301 gcccagttca ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc
    42361 ccgataactc tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac
    42421 tttttacatc tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc
    42481 gtcgaaatat tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg
    42541 gaatatctgg ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca
    42601 aatctgctga ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac
    42661 ctgtcactcg atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc
    42721 gaggcgcttt cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt
    42781 gctacgctct atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc
    42841 cggcctgaat tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt
    42901 tacaatcatc tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc
    42961 cgtaatggtt aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag
    43021 gccaggcgta caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg
    43081 agaaagtttt ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca
    43141 ggcggcaagg tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc
    43201 acattcaccc acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc
    43261 cgagagcggc gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc
    43321 gatggtaatt gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg
    43381 ctaatcactt ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc
    43441 catgaagaac gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc
    43501 aggtttaatg gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc
    43561 gcggcaacac caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt
    43621 gcggcgcagc caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg
    43681 ctgcccgcgt gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc
    43741 tcaagagaca ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg
    43801 cgcggggagt ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg
    43861 ctggtggcac cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca
    43921 gcaatggacg tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct
    43981 attagctggg taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta
    44041 attccccgca gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga
    44101 tggtggagct gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca
    44161 gccagcaggt tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac
    44221 atgatgttat cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt
    44281 cagcggcagt gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc
    44341 ggcaacagga attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc
    44401 attgatttcg acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta
    44461 atccagcaga ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa
    44521 tagcgcccag ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg
    44581 attaagaata tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag
    44641 cgcaatatcg accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt
    44701 tttgatggag ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat
    44761 cgccaaatct tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc
    44821 ttttatcagg gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat
    44881 tttctctttt aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg
    44941 tgaggcaaat gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg
    45001 aacgtcgctg accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc
    45061 accctgaccg atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag
    45121 aaggcgcgcg cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca
    45181 ggcgacgcca acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt
    45241 ttcccattca aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat
    45301 acgtttgccc gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc
    45361 aacaggatca atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac
    45421 gacttgtggc gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt
    45481 ctttgaactg taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc
    45541 agaagcgatg gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact
    45601 ataacctttc aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg
    45661 gcgggtacgg gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc
    45721 gccaagcttg ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc
    45781 aaaaccgcca ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc
    45841 ctgaccaacc acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg
    45901 cgagtcatcc tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac
    45961 gggggtctga tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt
    46021 acccgtcgac atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc
    46081 ttctgccagc gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac
    46141 caattgcgcc gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc
    46201 accatgatga cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc
    46261 gtcaagtgtc caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt
    46321 aaaaatcgcc agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata
    46381 accatgtgcg atagggctac gtacatattt cgcataacac atgcccgcca taacataatc
    46441 gtcagtatat cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc
    46501 acccgtagcg gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc
    46561 ttatggcaaa gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta
    46621 ggggaggtag gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct
    46681 gaataggggc agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg
    46741 attattacgc atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag
    46801 cgaatatttt gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac
    46861 cacgtttttc cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc
    46921 ttgtttttta agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt
    46981 atcgtgatgt tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc
    47041 gatacaggaa cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt
    47101 atttcgcgca ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc
    47161 ccttacacca ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt
    47221 acacgtaaat caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg
    47281 gtaccgaaat catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg
    47341 aaagttatca tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct
    47401 ccacggggta cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag
    47461 ctgcgtggct acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac
    47521 gcccataatg acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc
    47581 gccactatgg ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt
    47641 gcaacggcaa ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga
    47701 tggcgaatta atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa
    47761 tgccttacgc atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct
    47821 atacgagggt tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa
    47881 ttcaatcgct tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa
    47941 aagcgtttac gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt
    48001 tgttaaataa gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg
    48061 gaaatctgca ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac
    48121 aggggcagaa gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac
    48181 agataaaagt tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca
    48241 ctcagccaaa ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt
    48301 gttaacatta acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt
    48361 tattcttgct tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt
    48421 ctggctgagg gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt
    48481 agccgcaaaa aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa
    48541 gcggatgcag gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa
    48601 tgctgtgcat atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg
    48661 caagccgcag ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct
    48721 tatacaagca atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt
    48781 aaaatcggtt gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga
    48841 ttccggagca tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc
    48901 tggactggaa cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt
    48961 actacgcgaa gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca
    49021 cctcatagat aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc
    49081 aggttatctg gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat
    49141 ttatcctgca aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt
    49201 taaaaaacta gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc
    49261 aaatttttgg ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaag
//
LOCUS       DAZPBM010000010        48783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPBM010000010 DAZPBM010000000
VERSION     DAZPBM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.010"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHUZQ_000494"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHUZQ_000494"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1024285.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3121)
                     /locus_tag="ACHUZQ_000495"
     CDS             complement(1496..3121)
                     /locus_tag="ACHUZQ_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1024286.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3580..3666)
                     /locus_tag="ACHUZQ_000496"
     tRNA            complement(3580..3666)
                     /locus_tag="ACHUZQ_000496"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:gag)
     gene            complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHUZQ_000497"
     CDS             complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHUZQ_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1024287.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHUZQ_000498"
     CDS             complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHUZQ_000498"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1024288.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHUZQ_000499"
     CDS             complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHUZQ_000499"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1024289.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHUZQ_000500"
     CDS             complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHUZQ_000500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1024290.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHUZQ_000501"
     CDS             complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHUZQ_000501"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1024291.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHUZQ_000502"
     CDS             9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHUZQ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1024292.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHUZQ_000503"
     CDS             complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHUZQ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1024293.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10471..11904
                     /gene="dacB"
                     /locus_tag="ACHUZQ_000504"
     CDS             10471..11904
                     /gene="dacB"
                     /locus_tag="ACHUZQ_000504"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1024294.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11959..12066
                     /locus_tag="ACHUZQ_000505"
                     /pseudo
     CDS             11959..12066
                     /locus_tag="ACHUZQ_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHUZQ_000506"
     CDS             complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHUZQ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1024295.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13278..14243)
                     /locus_tag="ACHUZQ_000507"
     CDS             complement(13278..14243)
                     /locus_tag="ACHUZQ_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1024296.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHUZQ_000508"
     CDS             complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHUZQ_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1024297.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHUZQ_000509"
     CDS             complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHUZQ_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1024298.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15218..16189
                     /gene="ispB"
                     /locus_tag="ACHUZQ_000510"
     CDS             15218..16189
                     /gene="ispB"
                     /locus_tag="ACHUZQ_000510"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1024299.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHUZQ_000511"
     CDS             16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHUZQ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1024300.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHUZQ_000512"
     CDS             complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHUZQ_000512"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1024301.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHUZQ_000513"
     CDS             complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHUZQ_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1024302.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHUZQ_000514"
     CDS             complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHUZQ_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1024303.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHUZQ_000515"
     CDS             complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHUZQ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1024304.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHUZQ_000516"
     CDS             complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHUZQ_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1024305.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHUZQ_000517"
     CDS             complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHUZQ_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1024306.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHUZQ_000518"
     CDS             complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHUZQ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1024307.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21783..22760
                     /locus_tag="ACHUZQ_000519"
     CDS             21783..22760
                     /locus_tag="ACHUZQ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1024308.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHUZQ_000520"
     CDS             22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHUZQ_000520"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1024309.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHUZQ_000521"
     CDS             23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHUZQ_000521"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1024310.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24344..24919
                     /gene="lptC"
                     /locus_tag="ACHUZQ_000522"
     CDS             24344..24919
                     /gene="lptC"
                     /locus_tag="ACHUZQ_000522"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1024311.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24888..25445
                     /gene="lptA"
                     /locus_tag="ACHUZQ_000523"
     CDS             24888..25445
                     /gene="lptA"
                     /locus_tag="ACHUZQ_000523"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1024312.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25452..26177
                     /gene="lptB"
                     /locus_tag="ACHUZQ_000524"
     CDS             25452..26177
                     /gene="lptB"
                     /locus_tag="ACHUZQ_000524"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1024313.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHUZQ_000525"
     CDS             26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHUZQ_000525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1024314.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27681..27968
                     /gene="hpf"
                     /locus_tag="ACHUZQ_000526"
     CDS             27681..27968
                     /gene="hpf"
                     /locus_tag="ACHUZQ_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1024315.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHUZQ_000527"
     CDS             28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHUZQ_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1024316.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHUZQ_000528"
     CDS             28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHUZQ_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1024317.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29474..29746
                     /gene="npr"
                     /locus_tag="ACHUZQ_000529"
     CDS             29474..29746
                     /gene="npr"
                     /locus_tag="ACHUZQ_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1024318.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHUZQ_000530"
     CDS             29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHUZQ_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1024319.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHUZQ_000531"
     CDS             complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHUZQ_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR1024320.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHUZQ_000532"
     CDS             complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHUZQ_000532"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1024321.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHUZQ_000533"
     CDS             complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHUZQ_000533"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1024322.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34629..35558)
                     /locus_tag="ACHUZQ_000534"
     CDS             complement(34629..35558)
                     /locus_tag="ACHUZQ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1024323.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36233..40693
                     /gene="gltB"
                     /locus_tag="ACHUZQ_000535"
     CDS             36233..40693
                     /gene="gltB"
                     /locus_tag="ACHUZQ_000535"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1024324.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFCQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40706..42124
                     /gene="gltD"
                     /locus_tag="ACHUZQ_000536"
     CDS             40706..42124
                     /gene="gltD"
                     /locus_tag="ACHUZQ_000536"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1024325.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42308..42712
                     /locus_tag="ACHUZQ_000537"
                     /pseudo
     CDS             42308..42712
                     /locus_tag="ACHUZQ_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42804..43109
                     /locus_tag="ACHUZQ_000538"
     CDS             42804..43109
                     /locus_tag="ACHUZQ_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1024326.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHUZQ_000539"
     CDS             complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHUZQ_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1024327.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHUZQ_000540"
     CDS             complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHUZQ_000540"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1024328.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44518..45207)
                     /locus_tag="ACHUZQ_000541"
     CDS             complement(44518..45207)
                     /locus_tag="ACHUZQ_000541"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1024329.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHUZQ_000542"
     CDS             complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHUZQ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1024330.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHUZQ_000543"
     CDS             complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHUZQ_000543"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1024331.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHUZQ_000544"
     CDS             complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHUZQ_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1024332.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        12143 a        13125 c        12060 g        11455 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
     3121 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
     3181 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
     3241 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
     3301 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
     3361 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
     3421 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
     3481 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
     3541 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
     3601 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
     3661 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
     3721 ggctgagttt gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg
     3781 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
     3841 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
     3901 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
     3961 ccaacaaggc caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac
     4021 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
     4081 ctttcccact gaagtgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
     4141 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
     4201 aggcgacaat cgccgccttt ttagccagtt atttaccgtt ttaaacggct tttactgcat
     4261 cggcgatacg gtgtgcaaat tcagttacct gcgcttcgtc ttcaccttct accatcacgc
     4321 gaattaacgg ttcggtgccg gatttacgca acagcacacg tccacggcta cccagcgccg
     4381 cttcaacttc ggcggtcaca gctttaactg actcatgctc aagcggatcg ccgctacctg
     4441 cggtgtaacg cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
     4501 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
     4561 caccggtagt ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc
     4621 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
     4681 gaattcctaa ctgtttcaac gccagctcaa gccccatgtt actcatcaat gtacccacag
     4741 caccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
     4801 caccatcgac tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga
     4861 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
     4921 tagcccccac ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt
     4981 tcgcccccag ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat
     5041 ccaccacaat cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
     5101 cgatatagcg acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa
     5161 cacagctgat ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt
     5221 tggtgccgtc gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag
     5281 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
     5341 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
     5401 actccagcat atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt
     5461 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
     5521 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
     5581 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
     5641 ttgacgtcat gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
     5701 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
     5761 cgtgacatac ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
     5821 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
     5881 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
     5941 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
     6001 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
     6061 agggagcgga tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
     6121 ggtttggatg tatctaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
     6181 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
     6241 tcgccaccaa cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc
     6301 gcatctatca gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg
     6361 atccccatta cgtgaggatg gctaaggtcc agtgaagtgc cctgggcaaa gagtttcatg
     6421 gtgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
     6481 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
     6541 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
     6601 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
     6661 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
     6721 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
     6781 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
     6841 tcgctttcgc tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg
     6901 gacccaattt ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa
     6961 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg
     7021 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
     7081 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
     7141 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
     7201 cgtggtaagc cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
     7261 ccatcatgat tttatctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
     7321 cacgagcagc gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac
     7381 caggagtacc acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca
     7441 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac
     7501 ggtcgaaacg gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg
     7561 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
     7621 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
     7681 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
     7741 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
     7801 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcagcgtt ttaccggtac
     7861 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
     7921 tcggctcgcg cagatattca accagttcag caacttcttc ttttgcttcg tcgcagcccg
     7981 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
     8041 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaca
     8101 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
     8161 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
     8221 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
     8281 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
     8341 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
     8401 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
     8461 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
     8521 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
     8581 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
     8641 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
     8701 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
     8761 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
     8821 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
     8881 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
     8941 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
     9001 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
     9061 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
     9121 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
     9181 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
     9241 tatgggagat ggcggtagaa tgacccattt tcaatcccaa cgtaagcaaa aaatacgatg
     9301 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
     9361 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
     9421 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg
     9481 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
     9541 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
     9601 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
     9661 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
     9721 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
     9781 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
     9841 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
     9901 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
     9961 aaaataacgc gcccattgtt aggcattttg gtgacatcaa tcacctgcgc gtttgacagc
    10021 ttggcttcga tgtctttgat acggccttcg cagaagccct gctgttcacg agctgcgtgg
    10081 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    10141 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    10201 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    10261 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    10321 aaaataccga cccaggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    10381 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    10441 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    10501 accagctgta tagcgttcag tgttcaggcc gcaaatgttg atgagtacat tactcaactc
    10561 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    10621 gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg
    10681 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc
    10741 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    10801 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    10861 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    10921 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgccgc catagttgac
    10981 cgcaactgtt tctccgtctc gctctacagt gcccaaaaac ccggtgatat ggcctttata
    11041 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt
    11101 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    11161 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    11221 ggagccagtt atgccggtgc aattctgaaa gatgagttaa aacaggcggg tatcacctgg
    11281 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    11341 cagtcggccc cgctgcacga tctgcttaag attatgctga aaaagtcgga taacatgatc
    11401 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtacctgg tacatggcgg
    11461 gccgggtcgg acgctgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    11521 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    11581 atgcaggtgc tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    11641 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    11701 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    11761 attaccacag cgagcgggca acgaatggcg tttgtgcaat atctttctgg ctatgcagta
    11821 gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    11881 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggcttcag
    11941 attattgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccggccta
    12001 caaaatcgtg caaattcaac atattgcaat tctcttgtag gcctgataag cgtagcgcat
    12061 caggctgatt tggcgtttat catcagtgat taacgcttgt aaatgaactc aacgccttct
    12121 tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat cctcttcagc aatctcttca
    12181 agctgctggc gatgataatc gtcccacatg aattcgactt tctctggctg tttcgcttct
    12241 tcagcctgca cgaccgggtt ttcaaggata aaggtcatca catcccagca gagatctttc
    12301 acgcccagtc cgctcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca
    12361 gcaatcgctt tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac
    12421 actaaccaac gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata
    12481 atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac
    12541 aggacgcggc aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca
    12601 gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt
    12661 accacaccca gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc
    12721 gccgataccg cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg
    12781 tcagccagca gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt
    12841 ttctgccgtg gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag
    12901 ccgcccttag caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca
    12961 gtaccctggt cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc
    13021 ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca
    13081 aaacgataat cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca
    13141 tcaccaccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc
    13201 acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc
    13261 attttattct ccataaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta
    13321 cgtaatccgc cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca
    13381 aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc
    13441 agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc
    13501 acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta
    13561 ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcca
    13621 ggctttgcca gagggaagag cgcaattgta cataaagtgt acagtaaaaa caggatctgc
    13681 ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc
    13741 gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt aaatatctcg
    13801 accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc
    13861 tgagtgctgc gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc
    13921 gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac
    13981 aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc
    14041 ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc accaaggcca
    14101 atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc
    14161 tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg
    14221 ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt
    14281 cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg
    14341 ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg
    14401 tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg
    14461 tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg
    14521 atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct
    14581 gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc
    14641 tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatggccct
    14701 gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac
    14761 cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga
    14821 cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga
    14881 tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac
    14941 tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga
    15001 gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa
    15061 gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc
    15121 cattttttca gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca
    15181 cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga
    15241 gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga
    15301 cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg
    15361 tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac
    15421 cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga
    15481 tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag
    15541 cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc
    15601 actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct
    15661 gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta
    15721 tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg
    15781 tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca
    15841 gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta aaaatgtcgg
    15901 cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac
    15961 accagaacag gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct
    16021 ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc
    16081 cgaggaagaa gcagacaaag ccatcgcagc attacaggtg ctcccggaca ccccttggcg
    16141 agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt
    16201 catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct
    16261 ataacacctg aaaaaaaggg cgatgaatct ttatatattc tgaatattca cactctttac
    16321 aggaactttt tagagcaata ggccatcagg agtatagtga tgcccgacag aagaagtgtt
    16381 ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg
    16441 catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggagtct
    16501 cgcagaaatg gtttgagttc ctcaacgctg gcaaacgcat tatcgcgccc atggccgaaa
    16561 ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc
    16621 taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact
    16681 aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac
    16741 gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg
    16801 cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata
    16861 cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca
    16921 gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc atgcgcgccc
    16981 atacggctaa gctccggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat
    17041 ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat
    17101 gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg
    17161 ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg
    17221 gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc
    17281 gccaccagga aagtaccggt ttcgatacga tccggtagca cgcgatagac accgccgcct
    17341 aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc
    17401 agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata
    17461 atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg
    17521 actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa
    17581 ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc
    17641 ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct
    17701 accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg
    17761 cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca
    17821 cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagttttgg gacgttctgg
    17881 atctctaccg gttcttccgc cagtagagcg gcaaaaagga taggcagagc agcattttta
    17941 gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc
    18001 atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa
    18061 aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca
    18121 gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt
    18181 ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca
    18241 tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca
    18301 ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa
    18361 gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc
    18421 aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc
    18481 agaacatcag caggcaaatt atacaatttc gcgagggtat acactttgtc gtttaccccc
    18541 tgaagcgtca cgttgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc
    18601 agtccacccg tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc
    18661 gcttcctcac gcatttccca aagcggtagc agaacgtcct gatccagctc tccggataac
    18721 gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca
    18781 gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg atacctttgg
    18841 tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa
    18901 tcatgtcgta agcctgccag tttcccgtct gggagttttt acgccactgg aagtccagac
    18961 gcaccggcgg acggccattc gggtcaataa tggtaacgcg aataggcaca atggttttat
    19021 cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct
    19081 gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag
    19141 gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt
    19201 atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg
    19261 gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttataag
    19321 gattggtctg gtctgcctcg gttgccgcac tcagaggtgc aatcaccagc aaagcgacca
    19381 tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt
    19441 cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat
    19501 cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag
    19561 tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac
    19621 cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac
    19681 tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg
    19741 ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca atactgaccg
    19801 gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt
    19861 cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg
    19921 ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc
    19981 ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca
    20041 acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta
    20101 aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc
    20161 agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg
    20221 cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc
    20281 gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc
    20341 ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt
    20401 tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc
    20461 agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg
    20521 gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca
    20581 ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt
    20641 ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac
    20701 gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt
    20761 ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac
    20821 aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa
    20881 cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc
    20941 aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct
    21001 gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa
    21061 aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg
    21121 ccgcgccatc ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac
    21181 ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggtagtt gggtatgttc
    21241 gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg
    21301 aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat
    21361 attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg
    21421 gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg
    21481 cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg
    21541 catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt
    21601 tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg
    21661 tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat
    21721 ccgttacggc tttctgaaaa tcttcagcgg accgacgagt atacctgaag aaaggacgtt
    21781 agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg
    21841 accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca
    21901 tcggcatgac ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg
    21961 cgtctctgca cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca
    22021 atattttgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattccgatg
    22081 ttctgcgtcg tgaattaccc ttaatgttgt tggtcagcgt agtggccggt tccgtactct
    22141 atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc
    22201 tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca
    22261 gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg
    22321 gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc
    22381 tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa
    22441 ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg
    22501 ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg
    22561 cgctgataac gccaggagag attgagccat tggcgtacag tcgtgactac agcgtgatgt
    22621 tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg
    22681 gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat
    22741 cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt
    22801 tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag
    22861 cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa
    22921 gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa aatggccgca
    22981 acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt
    23041 gatttaggca tggtcacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc
    23101 agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc
    23161 accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta
    23221 gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt
    23281 atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt
    23341 gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt
    23401 atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcgttg
    23461 ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt
    23521 gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt
    23581 cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc tggcattctg
    23641 gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat
    23701 ggcgaccatt tactcggagt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa
    23761 agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc
    23821 ctgtcagcgc cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg
    23881 atggcgtact gtcagatggc ctgatttata tgggcaataa tggcgaagag ctgaaagcgt
    23941 tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta
    24001 tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc
    24061 acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg gaaaaactgg
    24121 cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg
    24181 aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg
    24241 attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac
    24301 tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg
    24361 ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcattg atatggccga
    24421 aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca
    24481 tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca
    24541 cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt
    24601 tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga
    24661 ccggatgctc tatttatatg gacacgttga agtcaacgca cttgtgccag actctcaact
    24721 tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga
    24781 agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa
    24841 cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa
    24901 caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg
    24961 catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc
    25021 ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca
    25081 tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag
    25141 tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg
    25201 ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg
    25261 gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg
    25321 taaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc accgttctgg
    25381 tgccgtcgca gctgcaggac aaaaacaaca aaggccagac cccggcacag aagaagggta
    25441 attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc
    25501 gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc
    25561 caaacggtgc cggtaagacc accactttct acatggttgt aggcattgtg ccgcgcgatg
    25621 cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc
    25681 gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc agcgtttacg
    25741 ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc
    25801 gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt
    25861 cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga
    25921 aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta
    25981 aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt gctgatcact gaccacaacg
    26041 tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg
    26101 cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg
    26161 aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct
    26221 gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct
    26281 ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca
    26341 ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg
    26401 cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc
    26461 ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg
    26521 caccagcggt gactacattg acgatgagct gccggtctat cagggcgaaa cgacgcagac
    26581 cttgcaggat tacctgatgt ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc
    26641 tattgctacc tctatcgtcg atgccgttga tgacatcggt tatctgactg tcccgctgga
    26701 agatattctc gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg aagccgtcct
    26761 taagcggatc caacggtttg atccggtcgg tgtagcggca aaagatctgc gtgactgcct
    26821 gctaatccaa ctctcccaat tcgataaaac cacgccgtgg ctggaagagg ccagactgat
    26881 cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac
    26941 gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg
    27001 ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg
    27061 taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat
    27121 caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat
    27181 ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac
    27241 gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggtga
    27301 agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc
    27361 gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact
    27421 gaagtatttc ttttccagtc acatcaatac cgagggcggc ggcgaagctt cctccacggc
    27481 gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga
    27541 cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc
    27601 gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc
    27661 caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg
    27721 aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa
    27781 tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac
    27841 tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca
    27901 ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac
    27961 aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc
    28021 catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg
    28081 aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta
    28141 cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg
    28201 cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa
    28261 aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag
    28321 atactctgcg cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca
    28381 tcgacaacca accggtggac ttactttttg ccctgctggt gtcggcagac caaactaaaa
    28441 cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc
    28501 gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta
    28561 ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt
    28621 acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg
    28681 cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc
    28741 tggctcgaac cctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata
    28801 tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac
    28861 cgcaactcct gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc
    28921 gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa
    28981 gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt
    29041 ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac
    29101 tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg
    29161 tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag
    29221 gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct
    29281 accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct
    29341 acttgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc
    29401 aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg
    29461 aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca
    29521 tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt
    29581 acgtaatgac gaaggcaccg aggccgaagc caacagcgtt attgcgctgc tgatgttgga
    29641 ttctgccaaa ggacggcaga ttgaagttga agcgaccggc ccacaggaag aggaagcact
    29701 ggccgccgtt atcgccctct ttaactctgg ttttgatgaa gattaatctt catcactttg
    29761 acatacaact cccttcaaac tccccccgac aataagaaaa tcacgtactg aaatcgttct
    29821 caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac
    29881 cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa
    29941 aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag
    30001 acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca
    30061 tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg
    30121 tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg
    30181 cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaaactct ccattacgct
    30241 gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact
    30301 gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca
    30361 gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat
    30421 tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca
    30481 agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga
    30541 tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg
    30601 gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg
    30661 acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac
    30721 agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg
    30781 ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac
    30841 cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag
    30901 cccttttcgt acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga
    30961 aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc
    31021 aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac
    31081 cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta
    31141 acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg
    31201 aacaggcgca acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat
    31261 taacagcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca
    31321 gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc
    31381 tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca
    31441 tcgacaggac agggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata
    31501 tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca
    31561 atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat
    31621 tcacggtcaa cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt cttcgccgcg
    31681 ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga
    31741 cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt ttccgtcatc
    31801 gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc
    31861 gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa
    31921 ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt
    31981 tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa
    32041 ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac
    32101 agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg
    32161 ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac
    32221 ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc
    32281 accgacgtta tcttcccagg caggaaggtc aggagactga atttgctgac ccagctgttg
    32341 cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat
    32401 gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat
    32461 cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg
    32521 ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt
    32581 actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac
    32641 agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt
    32701 cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tctcgcggat aacgcttcgt
    32761 cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca gtaacaccag
    32821 gtcgtattcg cccggtttaa acatctctag cgccgccttg ccggtcatgg cgacatcaac
    32881 gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc
    32941 caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac
    33001 ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc
    33061 gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc
    33121 aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc
    33181 gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg
    33241 cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt
    33301 gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac
    33361 ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt gttgcgcctg
    33421 caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc
    33481 aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc
    33541 cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg cggtgagttc
    33601 ggtatccagc agaatgcggc tcagaccgac gataccgttc agcggtgtac gcaattcgtg
    33661 actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata
    33721 ccgcttacgc tcggtaatgt ctcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg
    33781 gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag
    33841 ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac
    33901 ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc
    33961 gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt
    34021 acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg
    34081 ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt
    34141 ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat
    34201 attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg
    34261 caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa
    34321 gtagaccgcc cacggcgtaa tcaacaaacc aaagaagata gaacgaataa catcaatgct
    34381 ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac
    34441 gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac
    34501 ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc
    34561 ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc
    34621 tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca
    34681 tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacaga
    34741 ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt
    34801 ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc
    34861 aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg caacggatgt
    34921 agctttatac cgtcaacacc ggtttccact acccgctcca gcgtttgcag acattcagcc
    34981 agcccttcgc ctggcagacc gacaattaaa tgcgagcaca ccttcagccc gcgctggcgt
    35041 gccagctgcg tcgtgcgctg ataacaggcg aaatcatgac cgcgattaat gcgatgtagc
    35101 gttttgtcgt gcacggtttg cagccccagc tccagccaca cttcgtagcc ctggtcttta
    35161 tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgcgt gccgacacac
    35221 aaaccgacaa tattggcctg gctcaccgcc tgttgataca tcgaacgcag cacctgaact
    35281 tcggcaaagg tgctggtata cgcctggaaa taggcgaggt aacgtttcgc gcgattaatt
    35341 aaattcgcct gatgcgccag ctgttcggca atggaacgat gctgctgcgc ttcgtcggca
    35401 aacgaggcaa cattacagaa ggtgcagccg ccgcgcccga tggtgccgtc gcgattcggg
    35461 caactgaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga
    35521 tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga
    35581 aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc
    35641 ggcccgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca
    35701 taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa
    35761 aacagtagca tgaaacgtca ttaccaatta atgcagtata aaatgctggt tttctcgtca
    35821 attcaaggca ggataagggt taacacgcct ttatgacagt caggaattga ctgtttctct
    35881 aacgacttcc cttttagcct taaagataaa atctatttta atttcagcca tttaataaag
    35941 aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt
    36001 tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt
    36061 atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg
    36121 gcgaatgcga ggcgcgcgta tgacacgcaa cccccgtcgc cacgctcttt ctgtacccgt
    36181 gcgcagcggt tcggtagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta
    36241 cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga
    36301 acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg
    36361 cgcgattctc gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga
    36421 tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcgaaaa actacgccgt
    36481 cgggatgcta ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga
    36541 agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga
    36601 aggcgtactg ggtgaaatcg ccctctcctc tctgccccgc attgagcaaa tttttgtgaa
    36661 cgccccggca ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg
    36721 cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctat cgaatctggt
    36781 gaacatctat aaaggtctgt gtatgccggc ggatctgccg cgcttttatc tggatcttgc
    36841 ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca ctaacaccgt
    36901 accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg aaatcaacac
    36961 catcactggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgcttat
    37021 ccctgatctg cacgacgccg caccgttcgt caacgaaacc ggctctgact ccagttcgat
    37081 ggataacatg ctggaactcc tgctggcagg cgggatggat atcatccgcg ccatgcgtct
    37141 gttagtgcca cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt
    37201 tgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg tgatgtccga
    37261 cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc gctacgtcat
    37321 caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt accagcctga
    37381 cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggttatcg acacccgcag
    37441 tgggcatatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc atccatataa
    37501 agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga
    37561 agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta
    37621 cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt
    37681 cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta
    37741 cgactacttc tgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga
    37801 agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc
    37861 agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa
    37921 acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga
    37981 cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat
    38041 ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct
    38101 gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct
    38161 gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc
    38221 cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg
    38281 ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc tcaactaccg
    38341 taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc
    38401 ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct
    38461 gtgcttccag ggggcggtca gccgcattgg tggggcaagc tttgaagact tccagcagga
    38521 tctgctgaat ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc agggcggcct
    38581 gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct
    38641 gcaacaagcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga agctggttaa
    38701 tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt
    38761 caacattgct gatgttgaac cggcaagcga actgtttaaa cgcttcgata ccgctgcgat
    38821 gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat
    38881 cggcggtaac tcaaactccg gtgaaggcgg tgaagatccg gcgcgctacg gcaccaacaa
    38941 agtgtcgcgc atcaagcagg tggcttccgg tcgctttggg gttactccgg cgtatctggt
    39001 caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca
    39061 gttgccgggt gataaagtca ctccttacat cgccaaactg cgctattcgg tgcccggagt
    39121 gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact tagcgcagct
    39181 cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga
    39241 accgggcgta ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat
    39301 cgcaggctat gacggtggca ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg
    39361 cccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca
    39421 taagatccgt ttgcaggtcg atggcggcct gaaaaccggt gttgatatca tcaaagcggc
    39481 gattctcggc gcagaaagct tcggcttcgg cactggccca atggtcgcgc tcggctgtaa
    39541 atatctgcgt atttgccacc tgaacaactg cgcaacgggt gtggcaactc aggatgacaa
    39601 actgcgtaag aatcactatc acggcctgcc gttcaaggtg acgaattact ttgagtttat
    39661 cgcccgtgaa acccgcgagc tgatggcaca gctgggggta acgcggctgg tggatctgat
    39721 tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc
    39781 gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt aaggcactct actgcaccga
    39841 aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc
    39901 gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat atccgcaata ccgatcgctc
    39961 cgtgggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc
    40021 cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg tatggaacgc
    40081 gggcggcgtg gaactgtacc tgaccgggga tgccaacgac tatgtcggta aaggcatggc
    40141 gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag
    40201 tattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg
    40261 cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag aaggcattgg
    40321 cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta aaaccggcgt
    40381 taacttcggt gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt
    40441 ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca
    40501 tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag catactggct ctcagcgcgg
    40561 tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa
    40621 gtccagtgat gtaaaagcac tgctgggtca tcgtagtcgt agcgcagcag agttgcgcgt
    40681 gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca
    40741 gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta
    40801 cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa
    40861 cccatactgc gagtggaaat gtccggtaca caactacatc ccgaactggc tgaagctcgc
    40921 caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga
    40981 agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga
    41041 tgagtttggc gcggtgacca tcggcaacat tgagcgttat atcaatgata aagcgttcga
    41101 aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat
    41161 cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc
    41221 cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt
    41281 caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga
    41341 attcaaactc aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta
    41401 cgatgccgtc ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga
    41461 agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat
    41521 gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt
    41581 ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa
    41641 gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt
    41701 gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac tggggattga
    41761 agtgaacggt aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc
    41821 ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc
    41881 agatgcagtg atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca
    41941 cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg acaacgcctt
    42001 ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt
    42061 ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt
    42121 ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct
    42181 ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt aatacgctca tctggattgt
    42241 taccggtaga tgcccggcgt atagctgatt ccattattca acgcagcgcc atataaaggg
    42301 acagataatg gaatcgctct cagagagaac ctcaacaggc taccagcaaa tccacgacgg
    42361 tattattcac ctggtcgata gcgcccggac ggaaacggta cgtagcgtta acgcgctgat
    42421 gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc
    42481 aagggctgcg tatggtgcgc agctaatcaa gcgactatca aaggatttaa gtctaaggta
    42541 taagcgtggt ttctctgcaa aaaacttacg ccaaatgagg cttttttacc tcttttttca
    42601 acatgttgaa attcgccaga cagtgtctgg cgaattaaca ccattgggaa ttccgcagac
    42661 accatctgca gaattcccat cggcaaagat ttggcagacg ctgtctgcca aattgagagg
    42721 ctgtttgata tttccacagc ttttgtattg catctcctat tttcggtttg tatattcatg
    42781 caaataaacg aaggaaagtt gccgtgattt acattgaaca gcttgaatta atccataaaa
    42841 gtggtgatgt gttgtatcca gtaaaaataa ccagaaaatc atcaggaaaa actgccttcc
    42901 accttgttcc attcggctta aataagactc acgaccttct agaggtggaa gatgcttcag
    42961 aagcaattcg gctggtgatt gatgaacgtc attccattcg ctgcagtaca cttacagcaa
    43021 ctattacgaa taaaaagggc aagcgaatta agcggacagg tatatacagt atcaagggtg
    43081 ttaacataaa agaatacaac gtcaggtaat ggcacagagc ggtccgtcag ttcaggtttg
    43141 gctctgtgcc aaagatgggt caggtcacac ctgatctaat aaatttaagc cattaaatca
    43201 gccttaacct tcaccaccac ctttttaatc tcacccggct cgccaacaac gcatcccggc
    43261 ttatgcggct ctcctagcat aaacacggcg aacattcccg gtttcaggat gatggcttgc
    43321 tcgttttcaa tggtgctgca aagctggtaa tcatcctcat ggtggaactc ttcacactga
    43381 cgcgcagtgc ctgccatgcc aaacagaatc cgttcttcac cgtttaataa cagctggata
    43441 tcaatgtatt gctcgtgcag ctccgctttt ttctcaacgg gcgattgagt gttaaacgtc
    43501 atgacattca taaaaatatt gtcgccctgt aattcgtaac gacccggcgc tttttcttgc
    43561 ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac
    43621 ggtaatgacc gtacttcacc catcatcata atttttctcc ctgggccaac agcgctgccc
    43681 caagtaaacc tgcatcatgg cggtagtgcg ccgccagtaa atcaacatga aataccgctg
    43741 gctcctgcgc cagatacgtt tccaccaacg ccagataccc ttctgccaga ccaacgctgc
    43801 caccgaccac cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcca
    43861 gcacatgtgc ggagcggtga atcagttgct gcgcctgctc gtcgccctgc ccggcgcgtg
    43921 tgaaaatagt tctcgcatcc gcgcccatga gttcaccttg cgctgccgct gcaataccgc
    43981 gaccagaagc aatcgcttcc acgcaacctg tgcgtccaca gccgcagact gggccgtgtg
    44041 gatcggcaag cgtatgcccg atatgccccg ccagaccgcc agggccggta agcagtttac
    44101 cgccgctcac tacaccgccg ccgacgccag tggaaacggt gataaacacc atctcggtta
    44161 catcgccttc cagcgcctga tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa
    44221 tggtcggcaa attggtaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac
    44281 cgagattatg cggattcagc gccagcaaac tgccgtcgcg aataattccg gttgaagcga
    44341 tggcaacccg ctgcgcatga gcttgcaacg gagagactaa tgcggataag gcatcacgca
    44401 aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccgt
    44461 cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agtgtggtca
    44521 tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat
    44581 ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg
    44641 cgtgttgtaa cgcccttcgg caatcacccg acatccggct tcgctcaacg ttttcaccag
    44701 cgccagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat
    44761 aatttcggcg cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat
    44821 cgccagtaaa ccgtgatgat gtatacgtgc cagcagcgtt tcaacaggca ccggacgcgg
    44881 gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc
    44941 ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat
    45001 aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt cgatgcgaat
    45061 ggcaaccgcg cccgcctgct ctgccgctaa tgccatggct gcgacgattt cgggtttatc
    45121 gagcgggctg tcaggaatcg gctggcagga gacaatcagg ccaccgttag cagcgatttt
    45181 ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacatag
    45241 accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgct gccaaatggc
    45301 acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc
    45361 agccaacgct gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc
    45421 aggctgaacg agagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg
    45481 ccgatgattg gggccagtgc gccacccaat gcgccaacgt tgtaggtaaa gcccaggccc
    45541 gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc
    45601 ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg
    45661 ccaatcgcaa ataccggaat aatcagcagc tgcgaggcca gcaggctaca aacgtacgct
    45721 ttgcgggttc ccagccagtc accgaggaag cctcctacgc agcatcccac ggccgcgcca
    45781 aagccactaa agaacagcac attggcgaca gtatgcgggt tataagccag atcggttttc
    45841 agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc
    45901 accatcagca ttacgcccgt tggccagcgt ttgcctgtac tctgcaccat aaagctgata
    45961 aagattgcgg cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg
    46021 gcgaagcaga accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga
    46081 tgttcaccac ggtagagaat atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc
    46141 tctttccagt cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc
    46201 aaaatgccga taaagaacag cgcacgccaa ccccagaccg gaaccaccag gctatagacc
    46261 tgagcggcaa cgacggcccc cacagagaag cctgaaatca aaaaaccact ggctttgtta
    46321 cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc
    46381 atccccatgc cgatgaccag acgagcgata aacatggtga tgtagcctgg cgcaaagccg
    46441 caggccagcg tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg
    46501 tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg
    46561 gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt
    46621 accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa
    46681 aatgcgcgcc attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta
    46741 ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtatgaa aattgtaggg
    46801 tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaaa caactcaccc
    46861 gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt tttcatctac
    46921 cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat gcaggacagt
    46981 tttcaggccg cggaatacgc cagttttgat cagtaaatca atgactttgt tgcactcagt
    47041 ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacga tcccctgata
    47101 tcgccagccc atgatgttgt aagtactgcc gataccacca tcagcgcctg ccagcaagcc
    47161 ggaagcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt cacgacggat
    47221 ctgttccatc tgatagagat cgccagaagt ctgtttcagc gcaccgacgc caggcaacgt
    47281 aaccagggtg ttgatctgat ccagggtcag ttttacccca ctcagggctg gaatgttgta
    47341 cacaaccatc ggcaaaccat ccgctgaatc aataattgcc cgatagtgat cgcagtgttc
    47401 ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga agccataacg
    47461 tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga cgtgggcgat
    47521 gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt cacgctcgga
    47581 aaggctttgt acaaaggcct cgccggtcga accacccacg tataaaccgt cgatgccctg
    47641 ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt gttgttggtc
    47701 aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa atacctctga
    47761 agtgatactt gtctgataaa cgatatacct ttatacctgt tataccagat caattaagca
    47821 acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat tatttctttt
    47881 tgttggtggt ctgaccgaaa gcgtgccagg tagcagagac gctgttgaga tgcgattgca
    47941 acgcacgatc ggcttcgtca ggatcatgac ggcggatcgc atcaacgatc gcaatatgct
    48001 gttgatagct aacgttgtta tgctcgtgca gagcctgatc agccacggtt ggacgtgcgg
    48061 cgatcagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta ccagggatct
    48121 ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg ttatccagcg
    48181 actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg gtggcatgtt
    48241 cagccgcata gcgcaccaga ctggattcaa agaacagacg taattgttcg aaatgggcaa
    48301 tcccaccggg atgagaaagg aaatctttcg ccatgccgga aaactcaccg atgatagtgt
    48361 ccgcagaagg acgcgagacg cgagcgcgtt cgccgttgtt tatttgcacc agacctttgc
    48421 gttttaacgc tgccagcgct tcgcgcacag aaggacgccc gacgttaaag aacgccatca
    48481 gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg atcatctgtt
    48541 ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg ctacgcaagt
    48601 tgcgaccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca
    48661 ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga ttgaaagtag
    48721 gggaaaatgg caggtttttc tctttgtgcc tcatcattac cataattaac ggaataatta
    48781 act
//
LOCUS       DAZPBM010000011        46795 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPBM010000011 DAZPBM010000000
VERSION     DAZPBM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.011"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(16..867)
                     /gene="pdxK"
                     /locus_tag="ACHUZQ_000545"
                     /gene_synonym="thiJ"
     CDS             complement(16..867)
                     /gene="pdxK"
                     /locus_tag="ACHUZQ_000545"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1024333.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            972..1340
                     /locus_tag="ACHUZQ_000546"
     CDS             972..1340
                     /locus_tag="ACHUZQ_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1024334.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1343..2254)
                     /gene="cysM"
                     /locus_tag="ACHUZQ_000547"
     CDS             complement(1343..2254)
                     /gene="cysM"
                     /locus_tag="ACHUZQ_000547"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1024335.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2388..3485)
                     /gene="cysA"
                     /locus_tag="ACHUZQ_000548"
     CDS             complement(2388..3485)
                     /gene="cysA"
                     /locus_tag="ACHUZQ_000548"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1024336.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3475..4350)
                     /gene="cysW"
                     /locus_tag="ACHUZQ_000549"
     CDS             complement(3475..4350)
                     /gene="cysW"
                     /locus_tag="ACHUZQ_000549"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1024337.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4350..5183)
                     /gene="cysT"
                     /locus_tag="ACHUZQ_000550"
     CDS             complement(4350..5183)
                     /gene="cysT"
                     /locus_tag="ACHUZQ_000550"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1024338.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5183..6199)
                     /gene="cysP"
                     /locus_tag="ACHUZQ_000551"
     CDS             complement(5183..6199)
                     /gene="cysP"
                     /locus_tag="ACHUZQ_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1024339.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6370..7161)
                     /gene="ucpA"
                     /locus_tag="ACHUZQ_000552"
     CDS             complement(6370..7161)
                     /gene="ucpA"
                     /locus_tag="ACHUZQ_000552"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1024340.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7290..8147)
                     /gene="murR"
                     /locus_tag="ACHUZQ_000553"
     CDS             complement(7290..8147)
                     /gene="murR"
                     /locus_tag="ACHUZQ_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR1024341.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8311..9207
                     /gene="murQ"
                     /locus_tag="ACHUZQ_000554"
     CDS             8311..9207
                     /gene="murQ"
                     /locus_tag="ACHUZQ_000554"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1024342.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9211..10635
                     /gene="murP"
                     /locus_tag="ACHUZQ_000555"
                     /pseudo
     CDS             9211..10635
                     /gene="murP"
                     /locus_tag="ACHUZQ_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10640..11944
                     /gene="pbp4b"
                     /locus_tag="ACHUZQ_000556"
     CDS             10640..11944
                     /gene="pbp4b"
                     /locus_tag="ACHUZQ_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR1024343.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12002..12901)
                     /gene="yfeX"
                     /locus_tag="ACHUZQ_000557"
     CDS             complement(12002..12901)
                     /gene="yfeX"
                     /locus_tag="ACHUZQ_000557"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1024344.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12997..13572)
                     /locus_tag="ACHUZQ_000558"
     CDS             complement(12997..13572)
                     /locus_tag="ACHUZQ_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1024345.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13633..14082)
                     /locus_tag="ACHUZQ_000559"
     CDS             complement(13633..14082)
                     /locus_tag="ACHUZQ_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1024346.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14069..14494)
                     /locus_tag="ACHUZQ_000560"
     CDS             complement(14069..14494)
                     /locus_tag="ACHUZQ_000560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1024347.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14708..15577
                     /gene="amiA"
                     /locus_tag="ACHUZQ_000561"
     CDS             14708..15577
                     /gene="amiA"
                     /locus_tag="ACHUZQ_000561"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1024348.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15581..16480
                     /gene="hemF"
                     /locus_tag="ACHUZQ_000562"
     CDS             15581..16480
                     /gene="hemF"
                     /locus_tag="ACHUZQ_000562"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1024349.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16486..17538)
                     /gene="eutR"
                     /locus_tag="ACHUZQ_000563"
     CDS             complement(16486..17538)
                     /gene="eutR"
                     /locus_tag="ACHUZQ_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR1024350.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17584..18084)
                     /gene="eutK"
                     /locus_tag="ACHUZQ_000564"
     CDS             complement(17584..18084)
                     /gene="eutK"
                     /locus_tag="ACHUZQ_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1024351.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18097..18756)
                     /gene="eutL"
                     /locus_tag="ACHUZQ_000565"
     CDS             complement(18097..18756)
                     /gene="eutL"
                     /locus_tag="ACHUZQ_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1024352.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18766..19653)
                     /gene="eutC"
                     /locus_tag="ACHUZQ_000566"
     CDS             complement(18766..19653)
                     /gene="eutC"
                     /locus_tag="ACHUZQ_000566"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR1024353.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19674..21035)
                     /gene="eutB"
                     /locus_tag="ACHUZQ_000567"
     CDS             complement(19674..21035)
                     /gene="eutB"
                     /locus_tag="ACHUZQ_000567"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR1024354.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(21047..22450)
                     /gene="eutA"
                     /locus_tag="ACHUZQ_000568"
     CDS             complement(21047..22450)
                     /gene="eutA"
                     /locus_tag="ACHUZQ_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1024355.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22447..23673)
                     /gene="eutH"
                     /locus_tag="ACHUZQ_000569"
     CDS             complement(22447..23673)
                     /gene="eutH"
                     /locus_tag="ACHUZQ_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR1024356.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23990..25177)
                     /gene="eutG"
                     /locus_tag="ACHUZQ_000570"
     CDS             complement(23990..25177)
                     /gene="eutG"
                     /locus_tag="ACHUZQ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR1024357.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25167..26003)
                     /gene="eutJ"
                     /locus_tag="ACHUZQ_000571"
     CDS             complement(25167..26003)
                     /gene="eutJ"
                     /locus_tag="ACHUZQ_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR1024358.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26014..27417)
                     /locus_tag="ACHUZQ_000572"
     CDS             complement(26014..27417)
                     /locus_tag="ACHUZQ_000572"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR1024359.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27429..27716)
                     /gene="eutN"
                     /locus_tag="ACHUZQ_000573"
     CDS             complement(27429..27716)
                     /gene="eutN"
                     /locus_tag="ACHUZQ_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR1024360.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27823..28116)
                     /gene="eutM"
                     /locus_tag="ACHUZQ_000574"
     CDS             complement(27823..28116)
                     /gene="eutM"
                     /locus_tag="ACHUZQ_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR1024361.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28155..29171)
                     /gene="pta"
                     /locus_tag="ACHUZQ_000575"
     CDS             complement(28155..29171)
                     /gene="pta"
                     /locus_tag="ACHUZQ_000575"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1024362.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29168..29971)
                     /gene="eutT"
                     /locus_tag="ACHUZQ_000576"
     CDS             complement(29168..29971)
                     /gene="eutT"
                     /locus_tag="ACHUZQ_000576"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR1024363.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29968..30669)
                     /gene="eutQ"
                     /locus_tag="ACHUZQ_000577"
     CDS             complement(29968..30669)
                     /gene="eutQ"
                     /locus_tag="ACHUZQ_000577"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR1024364.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30644..31123)
                     /gene="eutP"
                     /locus_tag="ACHUZQ_000578"
     CDS             complement(30644..31123)
                     /gene="eutP"
                     /locus_tag="ACHUZQ_000578"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1024365.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31136..31471)
                     /gene="eutS"
                     /locus_tag="ACHUZQ_000579"
     CDS             complement(31136..31471)
                     /gene="eutS"
                     /locus_tag="ACHUZQ_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1024366.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHUZQ_000580"
     CDS             complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHUZQ_000580"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1024367.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34333..35283
                     /gene="tal"
                     /locus_tag="ACHUZQ_000581"
     CDS             34333..35283
                     /gene="tal"
                     /locus_tag="ACHUZQ_000581"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1024368.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35303..37306
                     /gene="tkt"
                     /locus_tag="ACHUZQ_000582"
     CDS             35303..37306
                     /gene="tkt"
                     /locus_tag="ACHUZQ_000582"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1024369.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37402..38445)
                     /locus_tag="ACHUZQ_000583"
     CDS             complement(37402..38445)
                     /locus_tag="ACHUZQ_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1024370.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHUZQ_000584"
                     /pseudo
     CDS             complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHUZQ_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHUZQ_000585"
     CDS             complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHUZQ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1024371.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41399..43099
                     /gene="narQ"
                     /locus_tag="ACHUZQ_000586"
     CDS             41399..43099
                     /gene="narQ"
                     /locus_tag="ACHUZQ_000586"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR1024372.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43263..46376
                     /gene="acrD"
                     /locus_tag="ACHUZQ_000587"
     CDS             43263..46376
                     /gene="acrD"
                     /locus_tag="ACHUZQ_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1024373.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHUZQ_000588"
     CDS             complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHUZQ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1024374.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10716 a        13154 c        12120 g        10805 t
ORIGIN      
        1 catcggcgcc attttttatg cttccgccag cggcggcaaa atcaattcat cgctctcatg
       61 ctgctgggtg tagcgcatca cttccagtac gcgcaacccc gcacggtgca ccgcatcgtt
      121 taacgccttc cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc
      181 tttcaggtcg gtttttaccc gtgaatggga aatgacattc acgctgtcgg cactgaccac
      241 caccacctgc atctcctgat tttcttcatt accggaggcg ctggtaatca ccacccattt
      301 taatgtgtct gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatt
      361 tttaccggtc aggatttcca gctcaaagat attgggggta attccttgcg ccagcggcag
      421 taaatattgt cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc
      481 gccaatcacc ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag
      541 ccactcggca aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcacg
      601 aagttggcgc agcgcatcac gctcctgaag cgcacgcaaa tagccgctaa accattcgtc
      661 cggaatcgca ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg
      721 cacggcaaag acattcaagc cgttctgttt tatagcaggc accgcaatgc tgttgcctac
      781 gctgccgtac actacctgcg actgcacggc gacgatatcc gcctgcagcg ccctactctt
      841 atcgttaaac aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg
      901 tattccaacc gaaactttac ctgattctgg cagtcaaatc ggctatcaca aaacaaggat
      961 aaggtaattc aatgaagaaa atcatttgtc tggtcattac actactaatg acactccccg
     1021 cttacgcgaa gttaactgtt catgaagaag cccgcatcaa cgccatgctg gagggattag
     1081 cacagaaaaa ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg
     1141 tttctcatct gcgtctgaag ctcggcaata cccgtaaccg cattgacact gccgagcagt
     1201 ttattgataa ggttgcttcg tcgtcatcga ttaccgggaa gccgtatatt gtgaagatcc
     1261 ccggtaagag cgatgagaac gcacagcctt ttttacatgc cttaattgcg cagacggata
     1321 aaacggtgcc tgcgcaataa tcttaaatcc ccgccccctg actaaaatgc tcttccccaa
     1381 acaccccggt ggaaaggtag cgatcgccac gatcgcagat gatcgctacc accaccgcac
     1441 cagggttagc ttttgccacc cgcagtgctc cggcaaccgc gccgccggag ctgacgccac
     1501 agaatattcc ttcccgcact gccagttcgc gcatggtgtt ttctgcatcg cgctgatgaa
     1561 tatccagcac ctcatccacc agagaagcgt tgaaaatccc cggcagatat tccgcaggcc
     1621 agcggcgaat gccgggaata ctgctgcctt cttccggctg caggccaaca atcgttactg
     1681 gtttggattg ttcgcgcata aagcgtgaaa cgccggtgat agtgccggtt gttcccatgc
     1741 tggagacaaa atgggtaatg cgcccgccgg tttgctgcca gatttccggc ccggtagtgg
     1801 tgtaatgggc gtaagggtta tcgggattat tgaactgatc gagcagcttt ccttcgccac
     1861 gattcgccat ctccagcgcc agatcgcgcg caccttccat gccctgctct ttggtgacaa
     1921 gaatcagttc cgcaccataa gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg
     1981 gcatcagcaa tttcatgcga tagcctttca gcgcggcaat cattgccagc gcaatgccgg
     2041 tgttaccact ggtggcttcg attaagacat cacccggttt aatttccccg cgcttttccg
     2101 cctcgacgat catcgaaagt gccgcacgat ctttcaccga acctgccggg ttattgcctt
     2161 ccagttttaa ccacacttca ctgccgttat ccggccccat tcgctgcaac ttcaccagag
     2221 gcgtattgcc tattgtttgt tctaatgtac tcacgatctc tatccatacg tggtgttgcc
     2281 tgatgcgacg cttgcgcgtc ttatcaggtc tacaggttac aaaccttgcc attaaaaagc
     2341 ccggatcgcg ggaagcgcct ccgggcgttt aacattcact caacctatca ggcgctttgt
     2401 gcgagagcaa gttcctcatc gcgggtttcg atacgctcgt cgccgttata cagccgcgca
     2461 tgttgcagac caacgaataa acgctcgcca cgctgcgggg catcgtcgcc atgcatcacg
     2521 accgtcagcg gttcgttgta ccaccccagc ggctgcacca ctaattgggt gtagtgacct
     2581 ttcgggctgg cttccagtac ctgtaccggc agcggcgaat cgagactggt acggcggctg
     2641 atatccactt cccacgggcg caggaagaga tccaccggcc cctgataagc aggtgtgtag
     2701 cccagcggcc agcgatgtgc gccaacatgg aactgcccgc cgcgaatggt tccctgcagg
     2761 cggttcactt cgcccataaa ttcgagcaca aaacgggtcg ctggttcgcg ccatacctgc
     2821 tccggcgcat cagcctgttc aatattgccc tggctcatca ccactacacg atcagctact
     2881 tccgtcgctt cttcctggtc gtgggtcacg aacacgctgg tgaatttcag ctcttcatgc
     2941 agttgacgca gccagcgacg cagctcttta cgcacctgcg catccagcgc gccaaacggt
     3001 tcatcaagca gcagaatttg cggttctaca gccagcgcgc gcgccagcgc cacgcgctgt
     3061 ttctggccgc cggaaagctg tgccggataa cgatccgcca gatgagcaag ctgcaccatc
     3121 tccagcaatt ttgtcacttt cgctttgatg gctgcggcat tcgggcgatc gcgacgcggc
     3181 agcaccgtca ggccaaaagc gatattgtcg aataccgtca tatggcggaa cagcgcgtaa
     3241 tgctggaaca cgaaaccgac cttgcgatcg cgcgcgtgca ggcggctgac gtcggtgccg
     3301 tggaagcgaa tatgcccgct ggtttgatgc tccagcccgg cgataatgcg cagcagcgtg
     3361 gttttcccgg aaccggacgg ccccagcaac gcgaccatct gacctgaagg aatatccagt
     3421 gagatatcgt tcagcacctg ggtgcgacca aacgacttct taatattggc aatctcaatg
     3481 ctcatgatgt tcctcctgct gtgcgcgttt ttcctgattc tccaggcgcc actgcaacat
     3541 actttttaaa aacagggtga taatcgccat cagcgttaac agtgccgcag cggtaaagga
     3601 gccgacggtg ttgtagtcct gctccagcaa ttcaatctgt aacggcagcg acagggtttc
     3661 gccgcgaatc gagccggaaa ccaccgacac cgcgccaaac tcgccaattg cgcgggcgtt
     3721 ggtcaacacc acgccataaa gcagcgccca gcgaatgttc ggcaatgtga cgcggcgaaa
     3781 catctgccag ccagacgcgc caagcaaaat cgccgcttcg tcttcctggc tgccctggct
     3841 taacatcacc ggcaccagct cgcgcaccac aaacggacac gtcacaaaga tggtgaccag
     3901 caccattccg ggccaggaga acataatttg cagattatgt tcatccagcc agccgcccag
     3961 cgggccgtta gagccgtaga acagcagata caccagaccg gcaaccaccg gcgatacggc
     4021 aaacggaatg tccagtagcg tcagcagtaa ctggcgtcca gggaagttaa agcgcgtcac
     4081 cagccaggcc agcagaatgc cgaacaccag gtttaccggt acggcaatca gcgcgatcat
     4141 caccgtcagc cagatggcgt gcagcatgtc cggatcggcc agattctgta aaaccggcat
     4201 cagccctttg ctgaacgcct gtacgaagat ataaatcatc ggcaccagca ggatgaacgc
     4261 cgataccagc atcccgatgc caatcagaaa ccatttgccc cagttaatcg ggcgcgcgtc
     4321 ataacgcttc aattgggtaa cttccgccat taatgaccta ccacacgccg accaaagcga
     4381 ctttgcagag tgttaattga gaacagcagc agcagagatg ccgcgaggat caccgaagca
     4441 atcgcgctcg ctgccgggta atcaaactcc tgtaagcgca caaaaatcat cagcgacgtc
     4501 acttccgtct tccacgcgat atttccggcg ataaaaatca ccgcgccaaa ttcaccaaga
     4561 ctacgggtaa acgacagcgc cacgcccgcc accagcgccg gagaaagttc cggcagcacc
     4621 actttgcaga aactctgcca gcgcgttgcg ccaagcgttt ctgccgcttc ttcatattcc
     4681 gggcctaact cttccagcac cggctgcacg gtacgtacca caaacggaat gctggtaaag
     4741 gccatcgcca ccgcaatccc cagccaggta taggtgactt tgatatcaaa cttcgccagc
     4801 cattcaccgt aaaaaccgtt tacagaaaag agcgaggcta gcgttaaacc ggcgactgcc
     4861 gttggcagcg caaagggtaa atccatcagc gcatcaagca gcgtgcggcc cggaaagcga
     4921 tagcgggtta agatccacgc catcagcagg ccaaaaacgc cgttaaaaat cgatgccaca
     4981 aacgctgaca gcagcgttac tttgtaggcc gctactacct gcgggttggt gatcacctcc
     5041 cagtactgcg cccagctcat ctgagccagc tgcatcacca gcgcggagag cggcagcagc
     5101 aaaatcaggc acacaaacag cagactggtg ccgaggctta aggtaaagcc cggcagcacg
     5161 cgtcttgatg aaactgcaaa cattaattac gccccgccgc taacagcttg tctaactcgc
     5221 cgccgctggt gaagtgggtt ttcatcactt ccggccagga gccaaatttg tcttccacgc
     5281 ggaacagctc ggtctgcggg aatttgtctt tcagtttatc catgacttcc gggttattca
     5341 cgcggtagta atagtcggtg atgatggttt gcgcctgcgg gctgtagagc cagttaaggt
     5401 aggctttggc ggctttttcc gtaccgttgg cctgcacgtt tttatcaacc cacgccaccg
     5461 ggaattccgc cagaatgttg gttttcggaa tcaccacttc aaaaccctgc gcttcatact
     5521 gtttacggat gttgttcact tccgactcga agctgatcag cacatcgccc aggccgcgct
     5581 cggcaaaagt ggtggtcgcg ccacgaccgc cagtatcgaa cacttcaacg tttttcagga
     5641 actgggtcat aaactgttcg gttttggctt tatcgccacc gtcagcttta tccgctgcgc
     5701 cccatgccgc cagataggtg taacgcgcgt tacccgacgt tttcgggttc gggaaaatca
     5761 gcttcacatc ggagcgcacc aggtcgttcc agtcgtggat attcttcggg ttgcccttac
     5821 gcaccaggaa gcccatggtg gagtagaatg gcgagctatt atttggcagg cgcgtttgcc
     5881 agtccgccgg gatcagcttg cctttgtcat gcaggatttg tacgtcagtc acctggttat
     5941 aagtgacaac gtctgctttt aagccctgca aaattgccag cgcctgtttt gatgacccgg
     6001 catgagattg ttttattgtc agtttgtcgc cgccgttttc tttggtccat tgttgctcaa
     6061 acggcgggtt cagggcggca aacagctcgc gggagacgtc ataagaactg ttcagcagtt
     6121 ccgttgcctg tacatggccc gccagcagca gagaagcgac cagcgcgagt gagttctttt
     6181 tcagtaagtt aacggccatt gcgcaccctt ataaatttaa tgactttcta atagccatca
     6241 tatttataac ggacgtgaaa ggagtaacgg ttttatatac cgtttggtga tttggaagtt
     6301 gaaaagggaa taagaaccgg gtttaaaacg aaatcctccc cacaaatgca gggaggaaac
     6361 agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc catcaatcac
     6421 attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg ccagttcgcc
     6481 gacttccagc ggatcggcga ggcgacgcag cgggattgct ttcgccattt cagtcagcac
     6541 cgactctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg gtgtgcgcac
     6601 gtatcccggg caaatggcgt taacgcgaat accagactgc gcgtactcca ccgccagcga
     6661 ttttgtcagg ccaacaatcg ccgctttcgt taaggcgtac gccgtttcgc caggatcggc
     6721 caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc gggcaatcat
     6781 ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatgt caatatggaa
     6841 atcgcgatcg tcatcgctca tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt
     6901 caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg cggctaccga
     6961 cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac cacacagttc
     7021 gtccgccagc ttttcgatct caggggagat atccagcaag attaggtttg cgccatgacg
     7081 cgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt
     7141 cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg ttacactcca
     7201 ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac taattcagga
     7261 aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag ctcactgctg
     7321 cgctgaatca ttttcaatga ctccacatca ttgagttgca caagaccaac aaacagtaaa
     7381 tcggtaacag aattttgcgc cgtgcgggta gacatcgacg agctacgcca ttcggtttca
     7441 ccggaaacgg tatcgagcgt gaaatgcgcc agccgccgca gcggagagtc agccagagaa
     7501 gtaatggcaa tcaccgttgc cccctgcttt cgtgccgctt ctgcgcacaa gacaatctct
     7561 tttttactgc cgctgtaaga gatagcgatt tgtacatcgc cttttttcaa cgcctgggaa
     7621 acggtggctt gtacgtgtgt gtcggcttca taggccacgc gatagccaat tttcatcagt
     7681 ttgaacgaca gatcgcgccc caccagcgcc gacccgccca ggcccgttat ctggataaac
     7741 ggtgctttac tgatgacctc aatgattttt tgcagccgag cgtagtcgaa caacgcacag
     7801 gtttgctcca gcgccagctc tttttcgcga ttcagtttgc gggcaatcac ttccagcgaa
     7861 tcatcactgg taatcgaact gtgcaggtgc agcgccgtgg cattcgtttt ctcccggctg
     7921 gcactgtatt cgccaattaa cgccatacgc aattcagtaa atccttgcgc gccgagcttc
     7981 tgggcaaatt tcacaatgct cgactgactg atgccgagct gtttcgccat cttgcgcgaa
     8041 gagacggatt tcagctcact cacgttggcc cgcagaaagt cagcaatttt ttgctcgttc
     8101 tcggtaaatt cactttccgc attgcgtatt ttcgtcaggt acaacatctt ccactctccc
     8161 gggctggcag caaaaattcc gcttaagttc ttatgacgct cttcacactc tgcaagtgta
     8221 gactcaatga ttcctttaag tctgtcaatt cgggatatat gattccatac atctgcaatt
     8281 atttgatcgt aaatagtaag gtcaccaccg atgcaacttg aaaagatgat tactgaaggc
     8341 tcgaacaccg cctcggctga aattgaccgc gtatcgacgc tggaaatgtg ccggattatc
     8401 aacgatgaag ataaaaccgt accgcttgcc gttgagcgcg tcctgccgga tatcgccgcg
     8461 gcgatcgatg ttatccacgc ccaggtaagc ggcggcggtc gtctgattta tctcggtgcg
     8521 ggaacatccg gtcgtctggg gattctggat gccagcgaat gtccgcccac ctacggcgtg
     8581 aaaccgggtc tggtggttgg tttgattgct ggcggcgaat atgccattca gcacgcggtg
     8641 gaaggcacgg aagatagccg agaaggcggc attaacgatc tgaaaaatat aaatttaacg
     8701 gcacaggatg tggtggtcgg tattgccgcc agcggtcgta cgccgtatgt gattgccgga
     8761 ctggaatacg cccgccagct cggttgccgc acagtgggaa tttcctgtaa tccggggagt
     8821 gccgtttcca ccaccgccga gtttgccatt acaccgattg taggtgccga agtggtcact
     8881 ggttcttcgc gaatgaaagc aggcacagcg cagaaactgg tgctcaatat gctttccacc
     8941 gggctgatga ttaaatccgg caaagtgttc ggcaacctga tggtcgatgt ggtcgccacc
     9001 aacgaaaaac tgcatgtgcg ccaggtcaat atcgttaaaa acgccaccgg atgtaatgcc
     9061 gaacaagcgg aagcggcgtt aattgcctgc gagcgcaact gtaaaacggc cattgtgatg
     9121 gtgctgaaaa atctcgatgc cgcagaagct aaaaaacgcc tggatcaaca cggcggcttt
     9181 attcgtcagg ttttagacaa ggaataaccc atggccaaag agatcagcag tgaacttctg
     9241 aacaccattc ttacccgtgt cggcggactg ggaaatatcg ccagttgtgg taactgtatg
     9301 acgcgcctgc gtctgggtgt acatgacagt tcactggttg atcccaatat caaaacgctg
     9361 gaaggcgtga agggcgtcat tttgaccagc gaccaggtgc aggtcgtttt tgggcctggt
     9421 aaagcgcatc gtgccgcaaa agcgatgagc gagctgctgg gcgaggcacc ggtacaggat
     9481 gccgcagaaa tcgccgccca gaacaaacgt cagttaaaag ccaaacaaac ctccggcgtg
     9541 caacaatttc tcgccaaatt cgccaccatc ttcacgccgc tgatccccgg ttttattgcc
     9601 gccggtctgc tgctggggat agcgacgtta attgccacgg tgatgcacgt tccggcagat
     9661 gctcagggca cgctacccga cgcgctgaat tttatgaagg tgttcagcaa aggtttgttc
     9721 actttcctgg tgattctggt gggctataac gccgcccagg catttggcgg cacgggcgtc
     9781 aatggcgcaa ttattgccgc actctttttg ctcggttata accccgctgc aaccaccggt
     9841 tactacgccg gttttcacga tttctttggt ctgcccatcg atccgcgcgg caatattatc
     9901 ggcgtgctga ttgccgcgtg ggcctgcgcc cgcattgaag gcatggtgcg ccgctttatg
     9961 tcggacgatc tcgacatgct gctgacctcg ttaatcaccc tgctgatcac cgctacgctt
    10021 gcgtacctga tcattatgcc gctgggtggc tggttattcg aaggtatgtc atggctgttt
    10081 atgcacctga acagtaatcc gttcggttgt gcggttttag ccgggctgtt cctgatcgcc
    10141 gtggtatttg gcgtgcatca gggctttatt cctgtttacc tcgcgttaat ggacagccag
    10201 ggattcaaca gcttatttcc catcctttca atggcaggcg cgggccaggt gggcgcggcg
    10261 ctggcactct actggcgggc gcaaccgcac agtgcgctac gcagtcaggt acgcggggcg
    10321 attattcccg gcctgctggg cgttggtgaa ccgctgattt acggtgtcac cctgccccgc
    10381 atgaagccgt ttgttaccgc ctgtttaggc ggcgcggcgg gcggtttgtt tatcggcctg
    10441 atagcctgat ggggtctgcc gatgggctta aacagcgcct ttgggccgtc tggtctggta
    10501 gcgctgccgc tgatgacttc cgcacaaggc atccttccgg ccatggcggt ttatgctggc
    10561 gggattctgg tggcatgggt ttgcgggttt attttcacca cgctctttgg ctgccgtaac
    10621 gtcaatctgg actgatatca tgaaacggac aatgctctat ctttctctgc tggctgttag
    10681 ttgtagcgtc agcgccgcaa aataccctgt tctgacagaa agctcgccag agaaagcagg
    10741 gtttaacgtc gaacggctta accagatgga tcgctggatt agccagcaaa ttgatgtcgg
    10801 ttatcccggc gtaaacctgc tgatcattaa agataatcag attgtgtatc gcaaagcctg
    10861 gggagcggcg aaaaagtacg atggcagcgt gttgatggaa cagcctgtca aagccaccac
    10921 cgggacgctg tatgacctgg cctcaaacac caaaatgtac gccacgaact tcgccctgca
    10981 aaagctgatg agcgaaggca aactgcatcc tgacgatcgg attgcgaaat atattccggg
    11041 gtttgccgat agtccaaatg acaccattaa aggcaaaaac accctgcgga tttctgacct
    11101 gctgcatcac agcggcggtt tcccggcaga tccgcaatac ccgaataaag cggtcgcggg
    11161 cgcgttatat tcccaggata aaggccagac gctggagatg atcaagcgca cgccgctgga
    11221 atatcagccc ggcagcaaac atatctacag cgatgtcgat tatatgctgc ttggatttat
    11281 cgtcgagtcg gttaccggtc agccgcttga ccgctatgtt gaagagtcga tttatcgccc
    11341 gctcggcctg acacatacgg tatttaaccc gctgctgaaa ggctttaaac cgcaacaaat
    11401 tgccgccacg gaattgaacg gcaatacccg cgatggcgtg atccatttcc cgaatatccg
    11461 cacctccact ctctggggtc aggtgcacga tgaaaaagcc ttttattcga tgggcggcgt
    11521 ttccgggcac gcaggtttgt tttccaatac cggcgatatt gcggtgttaa tgcaaacgat
    11581 gctgaacggc gggggctatg gcgatgtgca gctgttcagt gcggaaacgg tgaagatgtt
    11641 caccaccagc tctaaggaag atgccacttt tggcctcggc tggcgcgtga atggtaatgc
    11701 caccatgacg ccgacgtttg gcacgctggc aagcccgcaa acctacggtc acactggctg
    11761 gaccgggaca gtaaccgtta tcgatccggt caatcatatg gcgattgtga tgttaagcaa
    11821 caagccccat tcgccagttg ccgatccaca aaaaaatccc aatatgttcg aaagcggtca
    11881 gttaccgatt gcaacttatg gttgggtagt tgatcaggtg tatgcggcgt tgaagcaaaa
    11941 gtaataaaaa ggccagtcag ttttgttact ggctggcctt taatcatcga atcagaaacg
    12001 cttacaacgc cattaacttg tccagcgacg gcgcgaagta atagccgccg gttaccggtt
    12061 tggtgaaacg caacatcgca tcacgcttac catcggtatc gccaaacatg ctcagcagtt
    12121 gctgctcaat gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg tgagtgccac
    12181 tggcggtgcc gtacggcagg ctctggcgaa caatcttcag ccctttgcca tcttctttca
    12241 gatcaacgcg ggtgaggtga gaggtttccg gacgttcgtc gccgtcaatc tcttcgttgg
    12301 cctctttggt gcgcccgatc atcatctcct gatcgtgaac gctcatccgg ttgagctgct
    12361 tcaggttgtg ctcccaacgc tgcacaaaca catagctgcc gccggcatcc acgccgtctt
    12421 tgataaccgc cacttcgcga cgcgtctctt cacccgccgg gttttctgta ccgtcaacaa
    12481 agccgctcag gtcacgctct tccacccaac ggaagccgtg gatctcttct ttcacttcaa
    12541 tgcagtcacc aaaggcttcc atcgccgcct gggcgacaga gaagtttacg tcgtgacgca
    12601 gagaaagaat gtggatcaac acatcgaact gggtggtcgg cgcaaggcct ttaccgtagc
    12661 ccggaaaatc tttcagctct tctgccccaa cgccgccgct cagagcgcgc caggtgttgt
    12721 taccaaaggc aaccaccgca ccaagatgcg cgtccgggaa tttcgcttca aaagttgcca
    12781 gtttgtcggc aaatgtttta ctggccgcac gcagggcgtc aacttcccct ttcacgttgg
    12841 cttcgatcca aatcgccgcg cggcaatgtt ctggcaaaat gccactctga acctgagaca
    12901 ttgttcctcc tgaaaataag aatgccacgc aatccgtggc attgttgagc gctattttac
    12961 gtgttttttc gcggcggcgg tttgttcaga cgcaaattaa cgccgccaga taattttact
    13021 gactttccag tttttcaggg tatcgtccga aggcattaac ccttcaggac cacgccattc
    13081 cccgctgaac tggtagctaa tatgttgact gccttcggct ttacattcga cggcacgatt
    13141 atcatcacca tcagcttttt ggcaattgcc aaatgctttg ctgtaaaggt cgctaaacgg
    13201 tgtaccgatt ttaacaccgg tgtcagcagg aatatcgcta tccagcacgt caatgcgact
    13261 gatcgtgcct tgatcgccgt taatcaccat cgccacgttg tcgcctttca tcacttcaaa
    13321 aaagcgcacc acgttgccgt tcgcggtttt cattccgctg cgcaggcgat aatcgccatc
    13381 aagcgcatca gcaatggctt gttcttgcag tggtgtggac gccgttaatt cacccacgcc
    13441 ctgctcgctc actttggtgg acgatccaaa ccagttccac gggttagcgg ccgaccagtt
    13501 aactgacgac atcgtggaac agccggtcag catcaacggc atagcgcata acattaaacg
    13561 cagcgatttc atgtcacttc ctttggttat tcaataacgt tgcttggagt gcaaattcac
    13621 caaaaagtgc cgttattctt tttcttcatt aaaacaagcg cgtaaacgtc gattggtcag
    13681 cagccagatt aacgccacaa tatccgccac taccagcgcc agaccgatac cgctaacgga
    13741 ttcaccgttc agccacagcc acggttgcca gcaaagtaaa accacctgcg ccaacagcaa
    13801 cagaaaatag agcacatgcc aagtgcgagg aaatgtagcc cgccgaccgc tcaacaaaaa
    13861 cgccagcacc gccggaatgc ctggaatcag ccccagccag aaattatcgt gatcgggata
    13921 aaacagattt agcagcgcag taccctgctc gcgcgacgca ccggcaatga caaacagcac
    13981 ccaggttcgc gcctgaagca atagcacaag ccagaagagc aagggtaaac gcaggcgtcc
    14041 gtgcgcatca taatggacag gatggaactc agtactcttc atcttcaatc aaacgcttac
    14101 ccagactcag cacgtcggcg tgttcatatc ccaggcgttc atacattccg agcaccatgt
    14161 cgttatcttc cggcacattg atctgaattt tcgggcagcc acgagcaatc agctttttct
    14221 ccagccgatt aagcaacgca ttggcaatcc cacgcccacg aaactctgga tgcacgccaa
    14281 gataataagc agacccgcga tgcccgtcat aaccgcccat caccgttccg accacttcac
    14341 cgtttacctc agcgaccaaa aacaaactga cgtcatggtt catcttacgc tcgatgtcca
    14401 tttccggatc gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg atgacctctt
    14461 cgaaatcttc ctggcgaaat acgcgtatct ccatggtatt cgttaccttt ttgcgggtta
    14521 aaaggctgat tatggcgtga acggtcgaat tagccaatat ctgacgaaaa tcggttgaaa
    14581 aagtggcata atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat tctcaacatc
    14641 aaaccgtcgt aacggattac gcgatacgat ataacatctg gaactttatt attacaactc
    14701 aggccgtatg agcactttta aaccactaaa aacactcact tcgcgccgcc aggtgctgaa
    14761 agccggtttg gctgccctga cgttgccagg aatgtcgcaa gccatcgcca aagacgaacc
    14821 tttaaaaacc agcaacggac acagcaagcc gaaagccaaa aaatctggcg gcaaacgtgt
    14881 cgttgttctc gatccaggcc acggcggaat tgataccgga gcgatcggac gcaacggttc
    14941 gaaagaaaaa catgtggtgc tggcgattgc taaaaacgtc cgttccattt tgcgtaatca
    15001 tgggattgat gcgcgtttaa cgcgttctgg cgatacgttt atcccacttt acgatcgcgt
    15061 tgaaatcgcc cataaacatg gcgcagatct gtttatgtca attcatgccg atggctttac
    15121 caacccgaaa gctgccggtg cttcggtatt tgccctctct aaccgtgggg caagtagcgc
    15181 aatggcgaaa tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg gtaaaaaggc
    15241 gactgacaag gatcacctat tgcagcaagt gctgtttgat ctggtgcaaa ccgatactat
    15301 taaaaacagc ctgacgcttg gctcgcatat tctgaaaaag attaagccgg tgcataaact
    15361 gcacagccgc aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt cggttccttc
    15421 ggtgctggtg gaaacctcgt ttatcaccaa cccggaagaa gagcggctgt taggcacggc
    15481 ggcgtttcgt cagaaaatcg ccacagcgat tgctgaaggc gtgatcagtt atttccactg
    15541 gttcgacaac cagaaagcac attcgaaaaa gcgataagtt atgaaacccg acgcacacca
    15601 ggttaaacag tttctgctca accttcagga tacgatttgt cagcagctga ccgccgtcga
    15661 tggcgcagaa tttgtcgaag atagttggca gcgcgaagct ggcggcggcg ggcgtagtcg
    15721 ggtgttgcgt aatggtggtg ttttcgaaca ggcaggcgtc aacttttctc acgtccacgg
    15781 cgaggcgatg cctgcttccg ccaccgctca tcgcccggaa cttgccgggc gcagtttcga
    15841 ggcgatgggc gtttcactgg tagtgcatcc gcataacccg tatgttccca ccagccacgc
    15901 gaatgtgcgt ttttttattg ccgaaaaacc gggtgcagag cccgtctggt ggtttggcgg
    15961 cggcttcgat ttaacccctt tctatggttt tgaagaagac gccattcact ggcaccgcac
    16021 cgcccgtgac ctgtgcctgc catttggtga agacgtttat ccccgttaca aaaagtggtg
    16081 cgacgattac ttctacctca aacatcgcaa cgaacagcgc ggtattggcg ggctgttctt
    16141 tgatgatctg aacacgccag atttcgacca ctgttttgcc tttatgcagg cggtaggcaa
    16201 aggctacacc gacgcttatt taccaattgt agagcgacgt aaagcgatgg cctacggcga
    16261 gcgcgagcgc aattttcagc tctaccgtcg cggtcgttat gtcgagttca atctggtctg
    16321 ggatcgcggc actctgtttg gcctgcaaac tggcgggcgc accgagtcga tcctgatgtc
    16381 aatgccgcca ctggtacgct gggagtatga ttatcagcca aaagatggca gcccagaagc
    16441 ggcgttaagt gagtttatta aggtcaggga ttgggtgtaa ctccctcacc cccattcccg
    16501 catccgctga tgcaacgtca acgacggctt ctcggcaaac agctgctggt aatccgtggc
    16561 aaattgcccc agatgccaga atccccactg catggcggcg tcttttaccg ttgtgctttg
    16621 cgaccacggg cttatcagtt cgcggcgtac ggcgttcagg cgaatgcgtt tcagccacgc
    16681 gtttgggcca atgcctaaaa tagcgtgaaa cgcgttttgt agcgtgcggc gactgacatg
    16741 cagttgatta cacaagtcca gcaccgtcac cagctcagac atattttcca gcacatattc
    16801 acgggcgcgg gaaagtaatc tacggtaact ctgatgactg atgctttccg ccgtcaccat
    16861 tggttgcgcg tcttccagca tcgcccccat cgccattagc aaattatccc ccagcacttt
    16921 tcgcactgca ggctgatgga gattttccgg gttctcgcaa aacgtcgcca gcgcctgttg
    16981 gacaaagccc cacagcgcgg ctttatgctg ctctttcact tccagcgccg actggctacg
    17041 cagcatatgt aatacccgat ccggattgtg caaaaagtta gcctgacggg tgatgacatc
    17101 ttcagacagc actacgccca ggatcgtata atcgtccggc gtacttaatt caaactcggt
    17161 tccaccgggg cgcgtcgcta tttctgcgct tcccagacat tgcgaaccga taaatccctg
    17221 ctcaccgcgc gtcgccggaa tgccaaacca gaacgagttc ggccagacca ggcacgactg
    17281 acgcagcgcc agaccggtgt attcacgaaa aacctgaata tcatcgagca gaatttccgt
    17341 aaattcacca tgaaacttgc ccggatgcag ctgatcgtaa atctgctgcc aggcggtaat
    17401 cgtcaaagca tgttcataga catccgttgt ctgtcgttga tgaacattat ccacttcgac
    17461 cttcggcgtg agcttcaggt tttcgggtaa gggttcatga taaagatggt gcaaattggc
    17521 tgtacgggtc tttttcatga tgttaatgcc gggtgttgta ggacacccgg cacctccgac
    17581 aggttaatgg ggcttgagac gataacgact actgcgttta cgtaacgtcc cggcagaaaa
    17641 gagctgttcg agcgcatttc tggctttttc cagcggccag ccaaagtggg cagccacttc
    17701 tcctgccgtc attccctgac gtactgatgt taacagcgcc aacaactcgt cagcagattc
    17761 cgcgacgata actggcgcgt cgggttcctt tacgggttgc ttcggctggc ggttaaagcc
    17821 agtaaccagc cactgggtgt catcgtctgg ccgaccgatc tccttgcggc tgatgacacg
    17881 cccggtacgc atcgcggcag cacaacctgc gtccagcgcc gcacgacacg ccgccagatc
    17941 gccttccacc accagcgtta agcgaccagg gtcaagcact tcgtgactga gcagacgaac
    18001 gttagctgct ttgagcatgg catccgctgc atctatcgcg gcgaccatgc cgtccacttc
    18061 cagcaatccc agtgcattga tcatcggcaa cctccgttac gcacgctgga ttggattacg
    18121 cgcgatttcc agcactgcat cggtaaaggc gttacaggct gctttacacg cggcctggct
    18181 accggttaaa aatgctgccg agtagttggt ttcagacggt ggcgggacat aggtcaccag
    18241 ctgtacgtcg gcagatttca acgctgcatc aatgccgtag gtcgcttcca gcggcggtgc
    18301 caccagatac gccatcggat cgccaagcgt gatcccggcg gttgatgaaa gataagaacc
    18361 ggtacgcgaa actacatgtg ccaggaatgc ggtgttttct gcgtcattag cccactggaa
    18421 agcagcgcca ttttcaatat gcgcaaccat cgcatccaga ccagcacgca cttccgccgg
    18481 gtttggccca ccaagcataa tcagcacttc accggcagtc ggtgacggtc cgtgtgctgc
    18541 tcccgcatac agcgagcggc catataccac ttcgaccatc gcctgcttgg tcgcttcgtc
    18601 agccgcaatg taggtgacgt catcggaatc agcagaaatc agcccgaggc tacgaatatg
    18661 cggcggcaat tttagttcac gcgcaaattc agcgttaacg gaggcaatca cccgcatggc
    18721 ggttaccgac ggtcgaatca aatctaaagc tggcatgatg cctccttatc gggtcatgtt
    18781 gatgccggat gctttctgct ccagcatacg tttcgccaaa tccacaatga cggctgcggc
    18841 ttcaaccggc ggcgtgccgc cctggtgaat gttagagatg caggtgcgat cggcctcaac
    18901 ggtggtcgcc atacgcggcg agtaaacggc gtaacaggag aggctttctg actgacccaa
    18961 ccccggacgt tcccccacca gcaggatcac cactttcgcg ccgaggatct caccgatctg
    19021 atcttcaatc ttcacgcgac cataacgaac aaagaatggc gtgccgactt tcagcccggc
    19081 ctgtttcagg cccgccatca gcggcggcag gatctcttcg tagttcacgg tgatggcatc
    19141 agttgacagg ccatcagaaa taacgacctg tacgtccgga ttagcaacac actgtgcttt
    19201 cagcgcttca acagcttctg cacacaggcg gcggcccata tccgggcgag tcaggtagag
    19261 atttttgtcg ctaatttcgg agcgtacttc cagcaagccc tgcgctttca cccactcttc
    19321 cggcacttct ttcagtacgg tatctttcga acgagagtga tcggccagga aacgcagcag
    19381 cgcctgcgta cgtggacgcg gaccggtacg accggtacaa acgcgggcca cggtgctgcg
    19441 gcgcagttct gttaatacgt ctgcgcgatg cgggttttca acaccaatcc acgcttttgc
    19501 ttccgcagaa cctaaatcca gcgcgcagct ttccgaggtc accggtgccg cacagttagt
    19561 ggtggcgcac tttgcttctg acggcgcggg ggccgcttgt cccattgacg ccatcacgct
    19621 gcgtacaatt tcttcaatct gtttttgatc catgatgtgt tatctccgcg tcatcagaag
    19681 aacagtgacg gatcgcccgc ccgtttggtc aggcgaccgt ttgccataat gcccatgctt
    19741 tccagccagc gttcaaactc cggtgacggg cgcaggttga gtaactgacg cacagtggca
    19801 gtgtcgtgga atgcggtggt ctgatagttg agcatgatgt catcgcccag cggcatcccc
    19861 atgatgtagt tgcagcctgc ggtggcgagc aggatcatca ggttttcgtt gaggttctgg
    19921 tcagcgtcag cgtggttggt gtaacagcag tcacagccca tagagatgcc gctcagcttg
    19981 cccataaagt gatcttctaa gccagcacgg ataatctggc ggtcgttgta gagatactcc
    20041 ggcccaataa agccgaccac ggtgttgacg ataaacggat cgtaatgacg cgccagccca
    20101 tagttacgtg cttccatcgt cacctggtct gcgccgaagt tagcgccagc agacagcgcc
    20161 gaaccttgcc cggtttcgaa gtagaggcag ttttccccgg cgatacggtt gaactcagca
    20221 cccactgcgc gcgcttcgtc gagcatcgcc agttccacgc caaactcttt cagccctttt
    20281 tcgctgccac agatactctg gaaaatcagt ccgccaggcg cgccgcgacg aatcgcttcg
    20341 atctgggtgg tgacgtgcgc cagtacgcag ccctgagttg ggatgttgaa tttgtcgatc
    20401 acgccgtaaa tggtgtccaa cacgcggctt aagttttcca cgtcgtcagt taccgggtta
    20461 acgccgatca ccgcatcgcc caccccgaag gaaagccctt cgtagatttg tgcagcgata
    20521 ctttgcacgt cgtcacgggt gtcatttggc tgcaaacggg cgctgaaggt gcccggaata
    20581 ccgatggtgg tattggcctt tttgattacc ggcattttct tcgcgccgta gatcaggtcc
    20641 gcgttggagc aaatcttcgc taccgccgcg accacttccg aggtcagccc tttgcgggta
    20701 aaggcaatgt cgtccacgct ggtttcatcg ctcagcacat actcacgcag ttcgctgatg
    20761 ctcctgtttt taatctggtt gtaggccgtt tcgttaacat cgtcctgaat cagccgcgtc
    20821 acgcagtcat cttcataggc aatcaccgga ttattgcgga tgtccgctac ggtcatttcc
    20881 gacaacacct gctttgccgc cacgcgctcc tgtgagcttg ccgctgcaac gcccgccagc
    20941 acatcccccg aacgcagttc gttggcttta gccagcacct cttttacatc cttaaactga
    21001 tatacattgc cgaacaatgt ggtctttagt ttcataagtc gttccctcag gaaggaaatg
    21061 cgagtgattt caccgtcacc ggcacaaccg atccgccaaa aagaggcgta ccaatgtcga
    21121 tatagtcccc cgcgcggaca atcacttcgt caatgactgc caacgggagt tgttgtagct
    21181 gtgggcgcaa caacatgccc agagctttac caaagtcctg cccggccacc accagcaggg
    21241 gatgcggatt tggaaaacgc gcgacgaaat cgaccagcgc gttgatgacc gtcagtaccg
    21301 cggcgtaacg cacaggcagc gaggcgggaa gcgccagcac gtacgcgtca gttttgggat
    21361 caagatccag ctgaagcagc gcctgttgcc aggcactcac cagatccgtt tcatcaatcg
    21421 ggatcgccac cggcaaattg cgcagcggca gttgtacgcc ctcaagccag attgtgctgc
    21481 cagagagcga aagggtatgc gcgcccgcgc caatcaccgt ggcgcgcacg gtttgcgccg
    21541 gaaactgtac gttcatctca cgcaggcgcg gatggtcatg cagcgccgtt gccagcagcg
    21601 ggccaatatc ggcaaaacag aacgggtcgg cgggctggtg gcgataacat tcgcccacgc
    21661 cgccagaaag cgtaatgatt tcgggcgtaa cacctgcggg cagcaaaccg gtttgcatca
    21721 atgcctgcgc gagcggcgag agcgttccgt caatcacttc gacaatcagt tctgccatcc
    21781 gccgggtaac ctgcaccagc tgcgcgccgg tcagcgaacg ggcgtcggtg cctgcgccga
    21841 agcactcatc cacaatcatc tgccccggtt tatgagcgta aaccacgcgc ccctggctgt
    21901 cggtttccag caggcgacca ccgacgttga ggcaggcagt gccgctgatt tttccggcat
    21961 cgaacagggc gtagttcgca gtgccaccgc cgatgtcaat attcagtacc cgacacagcc
    22021 gttgttcaga aagggtttgt gccccggcac cgtgaccggc aatgacggat tcgaggtgcg
    22081 gcccggcgct ggcaacgaca aaatcgccca gcgactggga gagcaccatc accgccggac
    22141 gagcattgcg ggttttcgcg ctttcaccgg tgatgatgat ggcaccagaa tcaacgcttt
    22201 ccggcgcaat gcccgcagcc tgatattgct cgaggattaa ggttttcagt tccgcttctt
    22261 ttaaaccacc ctgtttatcg acaggggtaa agaacaccgg gctttgccag ctaatttcgc
    22321 gtttaatgaa ttcgtagcgc ggcacctgcg acaccgccgc acggttaacc agctccaggt
    22381 gggagaaaat cacctgggtg gtggtggtgc cgatatcgat accgacgctc aatagctggc
    22441 gagtgttcac gattgtgcct ccgcttcggt tttagccgtg gtcgcgtctt cttttggcac
    22501 cagcatcatc gccacgccaa tcgccgttac gccgccgatc aacttgccga caatcatcgg
    22561 gaagatcatg gcgttcatgt tggcagcggc gaagcctaag tggtcgccca gggcgaaagc
    22621 agcggaaacg gcgaaggcgc agttgatgac tttgccgcgg gtatccatct gcttcatcat
    22681 gccgaacatc gggatgttgt tggcaagcgt tgccaccatg ccggctgccg cgatgttgtt
    22741 catattcagc actttaccga cgctcatcag cggtttttca aaccagcggg tcagcagcag
    22801 caccattgga tacgccccta acagaacgca ggagatagaa ccgataactt caatggcgcg
    22861 catcacttca ccgggtttat cgccaggggc cataaagata gggtccagac cggggatcag
    22921 ttcccagcca agcagaaact taactaccgc ggcagcaaga ccgagggtga tcaatgcaac
    22981 gaggaatttg gcgaagatct ggaagccgtt gatcattttt tccgggatga atttcagccc
    23041 cagcgccacc agaatcgcaa caatgatcac cgggatcatg ttcatcagga tcagggcgaa
    23101 agtgaattcc accggctgac cgttgatctg cacaccggag tacatagcaa ccagaccacc
    23161 agcaatacag ccaatcggaa tggtcacaat gcccgccagc acgccgagcg ccagataacg
    23221 acggtcagaa ggttcgataa tgccgagcgc caccggaatg gaaaacacaa tcgttggccc
    23281 catcatcgac ccaagaatta acccagagta tagccacgct gctacgtcgc cgcccgccag
    23341 ctctttggcg aggaagaagc cgcccatatc gcacgccagc agcgttccgg cgaacattga
    23401 cgggtttgcg ccgagcattt cgtaaaccgg gataattacc ggcccgagta cgtgagccag
    23461 taccggtgcc agcgcggtca taccgaccat cgccagaccc agagcgccca ttgccataaa
    23521 gccttcttcg aactgaccgc ctgatccttc gatactttta ccgaacttac cgaggaaacg
    23581 agcagaaccg ccgaactgcg acaggatcct gtctacggca gctatcagca taaagaacat
    23641 catgatgtac atgatgattt cgttaattcc catcgccttt actccctgtt agttgttatt
    23701 tattggcgga tgcggcgtaa acgccttatc cgccctacat gtgcaatccc gaaggccgga
    23761 tcagacgcgg caagcgtcgc atccggcaat tgcaccgcgc cactggcgga tgcggcgtga
    23821 acgccttatc cgccctacat gtgcaatccc gtaggccgga taagacgcgg caagcatcgc
    23881 atccggcaat tgcaccgcgc cactggcgga tgcggcgtga acgccttatc cgccctacat
    23941 gtgcaatccc gtaggctgga taagacgcgg caagcgtcgc atccggcatt tattgcgccg
    24001 ccgcgtacag tccgacaatc tgttccaggc tggcggtacg cgggttactg cgcagacaaa
    24061 tatcttccag cgcggcctgc gcccatgcgc cgtaatgcgc agatgtcgca ccaacatcgc
    24121 ccagtcgttt accaatccca acttccgcaa tcagctcact taccgcgtta atagcgtcac
    24181 gatcgtcgga ttttttagtt cgcagtgccc gaccaatctg actaaagcgt tcacgacaaa
    24241 ccatccggtt aaattccatc accgttggca gcaacatggc gttcgccaga ccgtgtggaa
    24301 tatgcagcgc cgcgcccggc tgatgcgcca tcgcgtggca caacccaaga cccgcactgg
    24361 aaaacgccat tcccgccata catgaagcca gcaacatgct ctcgcgcgcg gcaaggtcgt
    24421 gaccgtagcc caccgctttc ggcagcgatt tgccaatcat cgcaatggca ccaatcgcca
    24481 gactgtcggt aaacggtgta gcgttcaggg cgctgtatgc ttcaatggca tgggttaacg
    24541 catcaatgcc ggtcatcgcc gtgacatgcg acggcacacc ttcggtcaac gcggcgtcga
    24601 gaatcgccac atccggcatc agcgaggcat gggctaacac ctgcttgcgc ccgctcaccg
    24661 cgtcgataat caccgttaca ttggtggttt cagagccggt tccggcggtc gttggaatgg
    24721 caatcagcgg caagcgcggt tgcagaacgc tggtttctga catctctgcc agcgtgctat
    24781 ccgggttcgt caccagcaac gccacggctt tcgccgcatc cagcaccgag ccgccgccaa
    24841 atgcgatcac cccatcacag cctgactcac gcaactgcgc cacggctgca cacacgtcgg
    24901 tgatgcacgg ttcgcccacc ggacatggcc agagcgtcat ggcgatgcct ttaacagcca
    24961 ggctgcgcgt cagcccggcg gtcatccctg cctgatgcaa aaagctgtct gccatcacga
    25021 acagatgttt cagcccacgc gtttgcgctt gctgtccgca actgctcacc gagcccggac
    25081 cgcaaagcgt caccggtgga acgctaaatg tttttacccg ttgcagattc agggtatcga
    25141 acgcctgaaa gagcgcggtc tgcaattcat tttgcatata gcccctccgc tttctctcgc
    25201 ccactactgg cgatcgccag cggggtcata aacaaactgt gctgcggtaa atgcacctgt
    25261 aacgccggga attgtttgcg aaacagttcc gccacgcccg gttgcataca agagccgccc
    25321 gccagccata aatcagtaat gccctgccct tcaatatgcc gggcgacaat gtccgccatt
    25381 ttttcgtaca ccggtttcac cgtaggccaa atctcttcac cgtgaccgcg cttgtactgc
    25441 tccgcctctt ccagcgaaat acggcgattt ccggcgagag tcagagagat gtgatgtccg
    25501 ccggttgctt catccgccga gtacgtcacc ttgccctttt tcacgatggc aatgccggta
    25561 gtgccgccgc cgatatccac tacaccggcg ttgtccagtt gcagcagatc cgcaactgcg
    25621 gttggctcat ccagcacatg gctcacttcc agtccagcag actccagcac gttgatggaa
    25681 atacgcgggt ccgtacctgg aggaaatgaa gtcgccgcat ggctaaaacg gcgaccgaat
    25741 tgctgctcga gcgtgtcgag atggcgacga acaatggtga cagcgccgaa gaaatcccag
    25801 acgatgccgt cgcggaccac atcggcccag tcgaggcata ccgccaccga ctgaccgtcg
    25861 cggtcgacaa ccatcgacac cacatcgcag gtgcccagat ccacccccag ccacagcgga
    25921 gattccgtcg cggcgggcgt ctggttacac agcgttgccg ccgtttgcag acgtggggtg
    25981 agccattgtt cgtcgtgcgc catctgttac tccttaaaca atgcgaaacg catcgactaa
    26041 tacacagcga cgcagacgga caaacgtacg cacgctagtt accccttcac cggttggcgt
    26101 ggtgatggtc atggtggtcc agccttcccc gcccagcccc agcccggcaa tgcgcggtcc
    26161 gttcttaacg aaaatgctgg tatcaatagc attcgccatc tggttcatgt tttcgatgtt
    26221 gcgcgagtgc attgccgccg tgtggtggca accgccttcc agtttcaccg ccagcgcaat
    26281 ggcatccgcc acgttggcga cgcgcacgac gggcagcacc ggcatcatca gttcggtcac
    26341 ggcaaacgga tgttctgcag tggtttccac aaacagcaaa cgcgtttctt gcggcacgtt
    26401 caggccgatt gccgccgcga ttttcgccgc atcgcgacca acccagtcac ggctgacggt
    26461 gccttttccg cgctcgtcga tatttttcag caacaccggt tgcagctgct gcgcctgttc
    26521 tgcggtcagt ttcaccgcgt gctggccttc catcagacgc atcagttcat cggctacgct
    26581 atcaacaaca atcagtacct tttcgtcggc acaaatgatg ttgttatcga aagaagcgcc
    26641 tttgacgatg gactgagcgg cacgggcgag gtcggcggtt tcatccacca ctaccggcgg
    26701 gttgccagcg cctgcggcaa tcagacgttt attggtgtgt ttacgcgccg cttctactac
    26761 cgcttcgccg ccggtcacca ccagaaggcc gatgcccgga aacttgaaca aacgctgcgc
    26821 ggtttcgata tccggatttg ccacagtaac cagtaagttt tccggcccgc ctgcggcaac
    26881 aatcgcctgg ttgagcagcg tgatcgcccg ctgggagact tttttcgccg ccggatgcgg
    26941 ggcaaaaatg acgctgttgc ccgcggcaat caggctgatg gcgttattaa ttacggttgc
    27001 cgccgggtta gtggacggag ttaccgaagc caccacgccc caaggcgcgt tttcaattaa
    27061 agtcaggccg ttgtcgccgg tcagcacctg cggagagaga cactcaacgc ctggcgtgcc
    27121 gcgcgcctga gcgacgtttt tggcaaattt atcttcaacg cggcccatgc cggtttcact
    27181 gacggcaagt tccgctaaat ctctggcgtg tttttcgcct gcctcacgaa tggcagcaat
    27241 ggctaactgg cgcattgcca cgctttttaa cccttgctgg gcgactttgg ctgccgcaac
    27301 ggcgtcatcc agggacgcga aaacgcccat ctcatgaacg gcggcgggcg gcgtgtcact
    27361 gctttgcatt ttcagcagta ccgctttcac cacctgttca atatcctgtt gattcatgat
    27421 gttctgcctt atttgtggaa aattacctga ccgccagaca ccacctcatc gacaatgcca
    27481 atcacgcaca gatcgaccgg tgacgtttcg cttttatgcg cctggcgggc ggaactgcca
    27541 ctcaccagta acacccactc cccggttccc gcgccaatat tgtcgatggc gacggcgcat
    27601 tgcccgtcag gattaccttg tggatcaatc atttccacca tcagcaattt gtcatgcgcc
    27661 agtccgtgat ggcgtacggt acaaacaatt tgtccagtga cgactgccag tttcataccc
    27721 gcctccgtgg cgtatttcag gtaaaagctc cccctaccct ccgcagaagg taaaatgaaa
    27781 aaggagagag cgtgacgccc gaatcgacgt cacacagggt gattacaggt tgctgctatc
    27841 gcctttcagg ccgatcggga agacttcttc caggtcgccg tgcggacgcg ggataacgtg
    27901 tacagatacc agctcgccga tacgctgtgc cgctgcggca cctgcatcgg tcgcggcttt
    27961 acaggctgca acatcaccac gaaccatggc agtacacagg ccgccgccaa tctgcttaac
    28021 accaaccagc ttgacgcgcg cagctttaac catcgcgtca gaagcctcaa tcagtgcaac
    28081 caggccccgg gtttcgatca ttcctaatgc ttccattgtg ctttcctctt tatcagggtc
    28141 cagaacggga ccgttcattc aaccagtgtt tgtaaactgc tttcgcggtt cacttctgtc
    28201 tgacgcggca cagctgccac cagcgccagc tcgataattt cctgcacgct acaaccacga
    28261 gagagatcgt gcatcggcgc ggcaagtcct tgtatcaatg gcccgacggc acgatatccg
    28321 ccgagtcgtt gtgcgatttt gtagccaata tttccggctt ccagcgacgg aaaaaccatc
    28381 acattggctt tgccctgtag cgggctggca ggcgcttttt gcgccgccac ttccggcacg
    28441 aaggcggcgt caaactgcaa ctcgccatca accaccagct ttggtgcgcg ctcacggacg
    28501 atttctgtcg cctgctggac gttggcaacg caggggtgac gggcgctacc gttactggaa
    28561 aacgacagca tcgccacgcg cggctcttct ccggtgaggg cgcgccaggt ttcggcactg
    28621 gcaagcgcga tatccgccag ttgcgccgcc gtcggctgtg gcaccacgct gcaatcggca
    28681 aagcccaacg ccggaccgct gtactgtggc agcatcagga aaatggatga gagcgtttta
    28741 cagcctggct gcaagccgat aatacgtaat ccggcacgca gcacattcgc cgtggaagag
    28801 aggttgcccg cgatacagac atccgcttta ccggcgctga ccattgctgc ggcgaacatc
    28861 agcggatcgg taagtttttc cagcgcatcc ggcggcgttt tttcgcccgc gcgggccagc
    28921 cagcgatgag caaattcttc ccgcattgcg aggttgccat gcggatcaat cacctgtagc
    28981 ccgtccatcg ccacgccgtg actgagcgca aactgacgaa gttcaaacgg attagcgacc
    29041 agaatgggcg ttgccagacc ttgttgatgt aaatattgcg cagctttcag cacacgttga
    29101 tctaacgcat ccggaaaaac cactctggcg ggcgctcgca acgccagttc acgacaacgt
    29161 tcaataatca ttgcgtttcc cccagtcgtt gttggatctg cttcaccgtt agcggcggct
    29221 tcgttacgct caggatcatc attacgtaga ccgtgctcga aaggcggttc agcgcctgca
    29281 agatgtccgg gcgtaatact tcaaagctgc gggtgataaa cacctgcgcc gctacggttt
    29341 cggtttcccg cactttggta cgtagcagat tgagcaacgc cgcatcgcgc ccgtggctgg
    29401 cttccggcac cagatgatcg tggtcgagat agcgcagcgg ctggtgagaa agtcgatgca
    29461 gatcttcgtc agaaagcccg acaatcgcct ggcagcccag cggttccccc agcgcatcgg
    29521 cgcgcataat gttgccaaga cgcgaacgga tatccgccag ccacggctgc cacggttccg
    29581 ccagttcaat ttgcagccac actgccagcg caatggtgct gtcgagaacc gcgcgaaaac
    29641 ccagacgcgg atcgcttttg gcgaccattt tttccgccga caggtgagtc agcgtatccg
    29701 gcttttttgc caccggctgg cggcacagtt cgcagcacgc ctgcggatgt tcatcgctac
    29761 tggtcagccc atgaacgggc tgcggctgct gctgttcatc gtcaacaaac aggcggccct
    29821 gctcgtcaat aaacttgatg cgcagatggc ggctttccag caactcccgg gcagagggcg
    29881 tcaggcgact gtccgcaggg agatggatct ctgcgccttc gctgagcgta tggttcgctc
    29941 ttagccatgc ttcggtgatg aaatctttca tagggattgc cagttagccg gccaggcgac
    30001 atacaggaat ttcacgctgg atgtcgtacc aaattcgatg ctggagcctt tcgggataaa
    30061 catcacgtcg cctgctttgg cgatcatggt ttcgccttcg tggcgaacat ggagctcccc
    30121 ttccagcacc atgtcgattt cgtcgtagtt cagggtccac gggaagaatg cgttctccca
    30181 ctgcataaaa ccggcggcca tgctgctgcc gtcgtctccg gtcacaagat cagtcaagcc
    30241 cacgcaatgc ggttgtgcgc catcaaaacg accaaacttg acgctgctgc catcaatcac
    30301 tttgatgccg cctttgccgg tcaccgattt gaagctcggc tgcattgcgc cctgctccag
    30361 cgactgtttt tccttcatca ctttttccat cagctgtgcg accaggcttt cggtaaactg
    30421 gccttccggc agctgggcaa tgatggtttc gcggatgcgc tggctttcgg ttttgtcggc
    30481 tggcacgctg gcgggcacgg acgccgttac cgggatcgat tcatcacatt cggtgatggt
    30541 aaaacccagt agatccgcca ctttacgggc ttccggggta ataatgctgg cgcgcagaac
    30601 cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga atatcattcg ctgtgatgag
    30661 ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc agttgctgca
    30721 cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaaaccc gtctcaagca
    30781 ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg atgacggcga
    30841 tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt tccgggtcat
    30901 ttgcgccatg gacatagatc agcatgtcta catcctgtag cgtggtaatt aaggcgtgat
    30961 accagcgggg atggctaaaa tattcacccg gcgtatcaat atcgccctta tcattaaatt
    31021 ccacggcctg tgtttttctg gcgagggtat aattcccctg caacgcatta aataacgttg
    31081 tttttcctgc cccgacgcta cccacaaacg caattcgttt catgaatgcc tcgtattaac
    31141 ttttggtcat ttcacaaagc gtataattta ataagcgacc taaaccgctg acggtttgtg
    31201 ataacgcttc ttctactgcg cccaccgagc cataaatcac caatgcaccg ctgaagcggt
    31261 cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa atcaccgacg atcatcgccg
    31321 tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg ccgattttct
    31381 tcgccagttc ctcaccggga tgcgcgatga gatgcgccag tgtgacctgt ttgccgggca
    31441 caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa actttcgtgt
    31501 atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt taaaaggtga
    31561 ggtagatcac taagaatgga gagatattcg ctgtggtgca taaactttca tatgcaacgc
    31621 atgaatataa ataaaataat atcgagatat tatatagact gctttttttt attattcgca
    31681 aactcagtaa taagctttac taatatattg cagccaaacc gcttaccctg aatattcagg
    31741 gtaagcgtga gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag
    31801 cgccaccatg ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt
    31861 cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa
    31921 gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt
    31981 caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgccg catcgccgtg
    32041 catttcacca tcaatcatca gttctggcgc acgttccctg accagttcca gcgcctgacg
    32101 cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac
    32161 gcgcggttca ataccgaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag
    32221 ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg
    32281 cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt
    32341 tttcaccacg ctaaaatgtt catgataatc acccaccgta ccgcaaatca ttgcatcggc
    32401 ttcgccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg
    32461 cgcctgttcc tgagtgacgc cgcgacgctt catgatctgg aagtattcgg tccagtactc
    32521 tttaaagcgc ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg
    32581 caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt
    32641 cgccagtccc agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc
    32701 cggcagaact acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag
    32761 gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg
    32821 acgcgtcgcc acgcccgact ccatcgccgc tttcgcgacc gcaggagcga tcttaacgat
    32881 caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgatc
    32941 gccatacgct gaagccacca cttcgctctg ttccgcatgg gccagttctg caatcgcgcg
    33001 taccgccgcc agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg
    33061 gaagatgaac gggaagcaaa ggacgttgtt gacctggttt ggatagtcag aacgaccggt
    33121 gcagatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg
    33181 attcgccagc gccagaatca ttggtgcccg agccattttc ttcaccattt cctgggtcag
    33241 cactttcggg ccggaacagc ccaggaaaat atccgcacct tcaatcacat catcgagggt
    33301 acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatgt ttggctcacg
    33361 gccttgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccag
    33421 cgctaccagc agattcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg
    33481 cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat
    33541 gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt
    33601 ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgattc cgccgaaggt
    33661 cggttcgagc gcagcgacaa cttcaataaa tttgtccggg tcgagttcat caacttcaat
    33721 gtcaaataca tcaatcccgg cgaatttctt aaacagaacg cccttgcctt ccatcaccgg
    33781 tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac
    33841 cgccaccagg ttaccgcggg cggtatattt gtaggctttt aacgggtctt tttcgatttc
    33901 aagacaaggt gcggcaacgc cgggtgagta ggccagcgcc agatcgcgct gcgttgccag
    33961 aggcttggtt ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc
    34021 actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag
    34081 tgtgtgggca acaccccctg cccacaacgt ggcgcacatt attaccttgc cggagtctac
    34141 agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa
    34201 cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc
    34261 gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa
    34321 ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac
    34381 agcggcgata ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg
    34441 ctgttactca aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc
    34501 tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg
    34561 gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg gtcgcgtgtc aacagaagtt
    34621 gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac
    34681 ttgtatcagc aacaaggcgt tgagaaatca cgcattctga tcaagctggc ttcgacctgg
    34741 gaaggaattc gcgcggcaga agagctggaa aaagaaggta ttaactgcaa cctgacgctg
    34801 ctgttttctt ttgcacaggc acgggcctgt gcggaagcag gcgtttttct gatttcgccg
    34861 tttgtcgggc gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg
    34921 gaagaagatc cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac
    34981 tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc
    35041 ggctgcgatc gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttcg
    35101 ccagtggtac gtaaattaat cccaccttct cagacgttcc cacgcccagc tcccatgagc
    35161 gaagcggagt tccgttggga gcacaatcag tatgcgatgg cggtagaaaa actgtctgaa
    35221 ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta
    35281 taaaccagcc acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact
    35341 cagtatggat gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc
    35401 tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg
    35461 gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt
    35521 gctgcatctg accggttacg acctgccgct ggaagagctg aagaactttc gtcaactgca
    35581 ttcgaaaacc ccaggccacc cggagattgg ttatacgcca ggcgttgaaa ccaccaccgg
    35641 tccacttgga caaggtttgg cgaacgccgt cgggctggcg atagcagagc gtacactggc
    35701 ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg
    35761 cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg
    35821 actgggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaacaga
    35881 aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca
    35941 tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt
    36001 gaaagataag ccgtcgctga ttatctgtcg tacggtgatt ggctttggat cgccgaataa
    36061 agcaggtaag gaagaggcgc acggcgcacc gctgggggaa gaagaagtgg cgctggcacg
    36121 gcaaaaactg ggctggcacc atccgccatt tgagatccct aaagatattt atcacgcctg
    36181 ggatgcccgc gaaaaaggcg aaaaagcgca gcagaggtgg aatgagaagt ttgccgccta
    36241 taaaaaggct catccgcaac tggcagaaga gtttacccgt cggatgagcg gtggtttacc
    36301 gaaggactgg gagaaaacga ctcagaagta tatcaatgag ttgcaggcga atccggcgaa
    36361 aatcgccacc cgtaaggctt cgcaaaatac gcttaacgct tatggcccct tgctaccgga
    36421 gctgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt
    36481 ttcgctgaag gaagatccgg cgggcaacta cattcactac ggggtgcgtg aatttggcat
    36541 gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt
    36601 cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg
    36661 gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca
    36721 ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg
    36781 cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac
    36841 ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa
    36901 agagattact cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattattct
    36961 gattgccacc ggttcagaga tggaaatcac cctgcaagct gcggagaaat tagcaggaga
    37021 aggtcgcaat gttcgcgtgg tttccctgcc ctcgaccgat attttcgacg cccaggatga
    37081 ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg
    37141 tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg
    37201 ttatggggaa tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa
    37261 tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatagt gatcgccgga
    37321 tgctgattgc cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc
    37381 tacatgtccc cgccattttg tttaacgggt gatccacaac gtgggccagg cgtctggccc
    37441 atgccagtta tcgcaggtgg gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc
    37501 aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac
    37561 cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg
    37621 cccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga
    37681 cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctga tcatcatcag
    37741 cgctttatca tcagtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca
    37801 gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttgcgtt cttcgagtga
    37861 gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac
    37921 gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg
    37981 ctgagcatca ataaacaaca acgccgcttt taagccactc agagaaatgg tctgattgcc
    38041 gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc aaaaacgcgg tgatggttgc
    38101 cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac ttaacgccaa
    38161 cggctcacca tctaacagca gccgtggcgc tatctctcct tcagacgcat ccggcgactt
    38221 caacccgccg cgctcaatac gtaaaacggc atcggtatgc gccccggcgc tgcggctcag
    38281 ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acgcagaagt tttggttatt
    38341 acaggtgacc tgccagtcgg aaaacgcccg ttgcgctagt gccgcccaca ccaaagacgt
    38401 cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc
    38461 agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatca
    38521 gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgttatttt tcagtccatt
    38581 aaatgtgacg tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg
    38641 gttttgatca tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct
    38701 tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg
    38761 attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc
    38821 acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta
    38881 tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta
    38941 gcgacacgga actgacgaat cagaaccacg gtctttttct tcgcgttgta caggaggatc
    39001 gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataactta gccatctttg
    39061 cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct
    39121 ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt
    39181 acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc
    39241 cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag
    39301 atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg
    39361 gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga
    39421 ctccagccac ggcatcccgt gtggattgaa gccaaacgcc ataatcaccg catcggctgg
    39481 catgacaaat tcactgccct caatcggtac aagtcgccgg cgcccctggg catccggctc
    39541 gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag
    39601 cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc
    39661 tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc
    39721 gccgtggcgc agcgcggtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac
    39781 gacgttgagt ccggcggtat tgataaacgg ctcttccggt agctcttcga gtcccatcac
    39841 ctgtttagtg ttggcgatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt
    39901 gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg
    39961 ttccaaaagc gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat
    40021 ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga
    40081 aggaatgccg aaagtgagca agccaccaat ttctggatgg cgatcgtaca ccgtcaccgc
    40141 cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacccg caccgataat
    40201 cgccacccgc ttgtccactt tggtgacatg gcttaagtca ggacgccaac ctttcgccaa
    40261 cgcctgatct gaaatgtagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg
    40321 aatagtacag gcaccttcac acaaacggtc ctgcggacaa acgcgtccgg taatttccgg
    40381 cagggtgttg gtcaggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag
    40441 ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc
    40501 gccgcattta aggcagcgcg aggcttcccg ttgtgcctga tcggcacgga atggcagata
    40561 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
    40621 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
    40681 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
    40741 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
    40801 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
    40861 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
    40921 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc
    40981 gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
    41041 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
    41101 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
    41161 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
    41221 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat
    41281 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
    41341 gactaaattg cgttttagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
    41401 gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
    41461 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
    41521 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
    41581 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcatc
    41641 ggtattaacc aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    41701 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    41761 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    41821 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    41881 tttcacgctg gtctttttta ctttgcgccg tatacgccat caggtggttg ccccgctgaa
    41941 tcagctggtt accgccagtc aacgtattga acacgggcag ttcgactcgc cgccgctgga
    42001 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga
    42061 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    42121 cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcg caggcgctga acacaagcca
    42181 gattgatgtg cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga
    42241 atatctggag ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga
    42301 attgccgatg cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg
    42361 gcaaaatagt cacgtttcat catcagaacc gctgcttaac agcgtttcgt cgatgctggg
    42421 acgcggtttg tactttaatc aggcgcaaaa gcattttcag cagttattgt tgatggaaga
    42481 acgtgcaacc atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg
    42541 tatccagttg acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat
    42601 catggccgat ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac
    42661 tactttccgc ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga
    42721 tacgttacaa aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc
    42781 gctggatgcg caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc
    42841 gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa
    42901 tcacacggtt tatatccgtg ataacgggat tggtatcggt gaaccgaaag aacccgaagg
    42961 tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt
    43021 ttcacaacct tccggcggcg gcacgttagt gagtattagc tttcgctctg cggagggtga
    43081 ggaaagtcag ttgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag
    43141 tataaaaggg catgataatt tacattaact cctttttttc tccacgattg gctcgtacct
    43201 tgccgctaca gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt
    43261 taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt
    43321 gtctgacagg gaccctggcg attttttcat tgcccgttga acaatacccc gatctcgcgc
    43381 caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca
    43441 ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat
    43501 ctcagagcag tggcatcggt caggcatctg tcactttaag ttttaaagca ggcaccgatc
    43561 cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc
    43621 aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca
    43681 ttgctttcgt ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca
    43741 gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt
    43801 cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg
    43861 ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg
    43921 gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc
    43981 tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg
    44041 taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc
    44101 gcttcaatgg taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa
    44161 tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg
    44221 gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg
    44281 tggtgaaaac gctgctggaa gctatcgccc tggttttcct cgttatgtat ctgttcctgc
    44341 aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct
    44401 tctccgtact ttacgccttc ggttacagcg tcaacacctt aaccatgttc gcgatggtac
    44461 tggcgatcgg tctgctggtg gatgacgcaa tcgtggtggt ggaaaacgtc gaacgtatta
    44521 tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg
    44581 gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtaccaatg gccttcttcg
    44641 gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc
    44701 tgtcagtact ggtagcgatg atcctcactc cggctctgtg tgccacacta cttaagccac
    44761 tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta
    44821 accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgc agcctgcgct
    44881 ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga
    44941 cgtcgttctt accgctggaa gaccgtggca tgtttactac ctcggtacaa ttgcccagcg
    45001 gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc
    45061 atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggtaacg
    45121 ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga
    45181 ccggcacctc gtttgccatt attgagcgtg caacaaaggc gtttaacaag attaaagaag
    45241 ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg
    45301 atatggagtt gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc
    45361 agttgctggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg
    45421 acgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg
    45481 ctatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact
    45541 ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc
    45601 tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct
    45661 ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg tctggaacgc tataacggtt
    45721 attctgcggt tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg
    45781 atattatgga atcgttagtg aagcagctgc caaacggctt tggtctggag tggacggcga
    45841 tgtcgtatca ggagcgactt tccggcgcgc aggctccggc gctgtacgcc atttccttgc
    45901 tggtggtatt cctgtgtctg gctgcgttgt atgaaagctg gtcggtgccg ttctcggtaa
    45961 tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg
    46021 aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg
    46081 ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag
    46141 cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta
    46201 tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg
    46261 tgggtactgg cgtaatgggc gggatgattt cagccactat tctggctatt tacttcgtgc
    46321 cgctgttctt tgtgctggtg cgccgccgct tcccgctaaa gccgcgcccg gaataagcaa
    46381 taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca
    46441 gcgggttctt gctgtttata ctttgtctca ggaattactt acgaagcata acttcgataa
    46501 agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta
    46561 ttctacggaa acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct
    46621 ccggggggac aaatcttatg taaatactat ggtcctgcgg tgatgatttg tatgtgatac
    46681 acagcaacat ttcgagatat tcatacggca tctaatactg atttaattca ggttaaaata
    46741 cagacagata acaagatgaa tattcttaat gtttacgtta aaaatgttta atatt
//
LOCUS       DAZPBM010000012        46186 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPBM010000012 DAZPBM010000000
VERSION     DAZPBM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.012"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHUZQ_000589"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHUZQ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1024375.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHUZQ_000590"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHUZQ_000590"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1024376.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHUZQ_000591"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHUZQ_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1024377.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHUZQ_000592"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHUZQ_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1024378.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHUZQ_000593"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHUZQ_000593"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1024379.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHUZQ_000594"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHUZQ_000594"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1024380.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHUZQ_000595"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHUZQ_000595"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1024381.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHUZQ_000596"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHUZQ_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1024382.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHUZQ_000597"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHUZQ_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1024383.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHUZQ_000598"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHUZQ_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR1024384.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHUZQ_000599"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHUZQ_000599"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1024385.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDCTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHUZQ_000600"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHUZQ_000600"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1024386.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHUZQ_000601"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHUZQ_000601"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1024387.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHUZQ_000602"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHUZQ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1024388.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHUZQ_000603"
     CDS             complement(14282..14581)
                     /locus_tag="ACHUZQ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1024389.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="ACHUZQ_000604"
     CDS             complement(14793..15233)
                     /locus_tag="ACHUZQ_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1024390.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="ACHUZQ_000605"
     CDS             15334..15933
                     /locus_tag="ACHUZQ_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1024391.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHUZQ_000606"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHUZQ_000606"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1024392.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHUZQ_000607"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHUZQ_000607"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1024393.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHUZQ_000608"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHUZQ_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1024394.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHUZQ_000609"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHUZQ_000609"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1024395.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHUZQ_000610"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHUZQ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1024396.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHUZQ_000611"
     CDS             complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHUZQ_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR1024397.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21614..22777)
                     /locus_tag="ACHUZQ_000612"
     CDS             complement(21614..22777)
                     /locus_tag="ACHUZQ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR1024398.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22777..23256)
                     /locus_tag="ACHUZQ_000613"
     CDS             complement(22777..23256)
                     /locus_tag="ACHUZQ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1024399.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23271..25718)
                     /locus_tag="ACHUZQ_000614"
     CDS             complement(23271..25718)
                     /locus_tag="ACHUZQ_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1024400.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25711..25866)
                     /locus_tag="ACHUZQ_000615"
     CDS             complement(25711..25866)
                     /locus_tag="ACHUZQ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR1024401.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25863..26138)
                     /locus_tag="ACHUZQ_000616"
     CDS             complement(25863..26138)
                     /locus_tag="ACHUZQ_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1024402.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26195..26713)
                     /locus_tag="ACHUZQ_000617"
     CDS             complement(26195..26713)
                     /locus_tag="ACHUZQ_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR1024403.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26726..27916)
                     /locus_tag="ACHUZQ_000618"
     CDS             complement(26726..27916)
                     /locus_tag="ACHUZQ_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR1024404.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28327..29745
                     /locus_tag="ACHUZQ_000619"
     CDS             28327..29745
                     /locus_tag="ACHUZQ_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024405.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29860..30387)
                     /locus_tag="ACHUZQ_000620"
     CDS             complement(29860..30387)
                     /locus_tag="ACHUZQ_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1024406.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30391..32709)
                     /locus_tag="ACHUZQ_000621"
     CDS             complement(30391..32709)
                     /locus_tag="ACHUZQ_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1024407.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32720..33250)
                     /locus_tag="ACHUZQ_000622"
     CDS             complement(32720..33250)
                     /locus_tag="ACHUZQ_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR1024408.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33243..34151)
                     /locus_tag="ACHUZQ_000623"
     CDS             complement(33243..34151)
                     /locus_tag="ACHUZQ_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR1024409.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34156..34503)
                     /locus_tag="ACHUZQ_000624"
     CDS             complement(34156..34503)
                     /locus_tag="ACHUZQ_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR1024410.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34500..35135)
                     /locus_tag="ACHUZQ_000625"
     CDS             complement(34500..35135)
                     /locus_tag="ACHUZQ_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1024411.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35202..35654)
                     /locus_tag="ACHUZQ_000626"
     CDS             complement(35202..35654)
                     /locus_tag="ACHUZQ_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1024412.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35647..36114)
                     /locus_tag="ACHUZQ_000627"
     CDS             complement(35647..36114)
                     /locus_tag="ACHUZQ_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1024413.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHUZQ_000628"
     CDS             complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHUZQ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR1024414.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHUZQ_000629"
     CDS             complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHUZQ_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR1024415.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36635..37060)
                     /locus_tag="ACHUZQ_000630"
     CDS             complement(36635..37060)
                     /locus_tag="ACHUZQ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR1024416.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37075..37572)
                     /locus_tag="ACHUZQ_000631"
     CDS             complement(37075..37572)
                     /locus_tag="ACHUZQ_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR1024417.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37572..37853)
                     /locus_tag="ACHUZQ_000632"
     CDS             complement(37572..37853)
                     /locus_tag="ACHUZQ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1024418.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37857..38060)
                     /locus_tag="ACHUZQ_000633"
     CDS             complement(37857..38060)
                     /locus_tag="ACHUZQ_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR1024419.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38060..38569)
                     /locus_tag="ACHUZQ_000634"
     CDS             complement(38060..38569)
                     /locus_tag="ACHUZQ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1024420.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38669..39412)
                     /locus_tag="ACHUZQ_000635"
     CDS             complement(38669..39412)
                     /locus_tag="ACHUZQ_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR1024421.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39416..40488)
                     /locus_tag="ACHUZQ_000636"
                     /pseudo
     CDS             complement(39416..40488)
                     /locus_tag="ACHUZQ_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40547..41401)
                     /locus_tag="ACHUZQ_000637"
     CDS             complement(40547..41401)
                     /locus_tag="ACHUZQ_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1024422.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41575..43347
                     /locus_tag="ACHUZQ_000638"
     CDS             41575..43347
                     /locus_tag="ACHUZQ_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR1024423.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43347..44381
                     /locus_tag="ACHUZQ_000639"
     CDS             43347..44381
                     /locus_tag="ACHUZQ_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1024424.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44753..45895
                     /locus_tag="ACHUZQ_000640"
     CDS             44753..45895
                     /locus_tag="ACHUZQ_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR1024425.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45897..>46186
                     /locus_tag="ACHUZQ_000641"
     CDS             45897..>46186
                     /locus_tag="ACHUZQ_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFR1024426.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAICLIHETIYDRNVEFDKLRKNLRSE"
BASE COUNT        10893 a        12406 c        11844 g        11043 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattgca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agccaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccgcgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaccc ccacatcatg
    21181 tgggggaaga cagggatggt gtcacaaaaa agtacctaac ctgatgatat aaatggattt
    21241 atgtcaaccg ctgtccacat agcgtccaca tcgaccataa ataagcccct caactgaggg
    21301 gctatttttt tgatcacatc cacataattt gctgccctga cggcaacggg tgcggcctta
    21361 cggcgtggac ttctcccggc ttcacgatgt atcgctgtac cgactcataa gtgatgaacg
    21421 tggcgctgca attcacgttc tggcactggt gataacgctc ttttgtcgtg tcagtgatat
    21481 agcgacttgt acgcgcatgt gcggcatgct ggcataaagg acaatgaaac atcgcgagca
    21541 cctcttccgg ttttgttgat ggtgccattt tagttaattt atccttataa aacaaacaga
    21601 taaataaacc acgtcactca tcatcttctg tttcgtactc cacatcagaa agccttacct
    21661 caagctccaa ggacgtcgtg aagccgctat tattgagaca atgtgtcacc ttagtgatgg
    21721 tccagtcctg ctcgtctatg acgcgcttaa agcctgacac tttaaccggt gtttccgtgt
    21781 aaatatctgc acgaccggta gccaggctga tggagaactc cgctacaccc cgttgcagtt
    21841 tatcccactt cgcctgagcg gcgcgcatgg cctgcgcttt tgtggcatat accgtggtca
    21901 gggcaaaaac gttgtcagct tcaccggcca tgtattcacc ttcgcgcgct tccggtactt
    21961 ttggcgcttt cttctgcgtg accggtttcg ctttcgggtg ctccagtgcg cgcaggtgtt
    22021 tttctttctt tttgcgtttc agttttacct tctgcttttg cggcttcggg tctttggtgt
    22081 gtaaccactt tgccgttacg ccggtgtaag ctccacggtc agcaatcgca aaatgatgac
    22141 ggtcgccgtc gctgcgggtg atggtaatct gcgggatttt tttaccgctg gccgtcaccc
    22201 cctgccccgc tttgagaaac agcagttttc ccatttttac cgacacctcg ccgccgttgc
    22261 gttcagcaag acgggtcagg aatttcgcat cagactcctg cgactggtcg atgtgtggaa
    22321 tttttattcc ggccagtgac ggagcgacac tggcttccag cctgttacgg gaggctatcg
    22381 cctcaacaat cgcaccgagc gtaatgtcat gccatgagcc ttcacggcgg gaattgagcg
    22441 tcccgcgaaa atctgcactc cgggcgcgga tggtgaccac atccggcgcg ccccggtgtt
    22501 caacctcatc aacggtaaat ttccctttgc ataccagggc aaaacctttc cagccgatat
    22561 acaccgtcag gacagcgcca cgaaccggca gcccgacctg cccgtcggca tcgttcagtt
    22621 caatatcaag ctggtcagcc tcaaagcccc ggttatccgt cagggtcatg ctcatcagac
    22681 ggtcgctgat attgccggta atatccctgc tgtcgagcat cagcatgtaa tccggcgtca
    22741 gcgtactgcc tgcatcaaat gtcagcgcat ccagcattat cccgcccccg tcatacccgt
    22801 gaatttagtc gccatactgc cagccttacc gatgagcgat tccgcctgtt taccgatatc
    22861 gccataaagc gcggccagtg actcatcaac gcgggtgagc gacagcgtaa aatcaatttt
    22921 ccgtggtgtg ccgtctgcaa agaaaatact ccctgtttca ctcaccttgc tgatgacata
    22981 catgccgtaa atcatgccgg tgccatccag caacggccac gcccggcctt cctctgccat
    23041 cagtctgagc gtggtcatcg tcagctttcc gccagtcagt tcgggataaa gcacaccggc
    23101 aagtgtgatg ttctcctcac ctacaccgag aaactgatag gcatcccgtt taccgatacg
    23161 ggaatttgac ggccagcgat aatctgattc acgctgcatg gtctggtgtg gcagcgtctg
    23221 gcgcataaaa acaaacatac ctaacgcgag catcattttt cgtcacctcc ttaaccgtca
    23281 tgcatcatgc tggcacgggc gcgcgcacgt ttatcccgct cgtatttttc gagcgcatcc
    23341 tgtaactggc ggtcaagctg tgtccccggt gcagtaccac ccgtcaggct gatgtgatat
    23401 tcgtttttac tctggtccac ataagagcgg ccagccggtg ccgtgaccgg ctgataagcc
    23461 tgatagcctg cataagagct ggtcgccgga atataaccac cggtgccata cgtggcggca
    23521 tgagttctgg cggcggtctg gtcaagtgtg tctgactctt tgttgatgac cccgagcttt
    23581 tccagtaccc agtcaatgcc gctgcgcaat ttgttgaacg cattaagcgg cagcatcagc
    23641 gcgtcagcca gtgcctgccc gaacatgacg cccgtgtcac ggcaacggtt cagggtgtcc
    23701 tgggtggctt tgaccggggc aatcaggttt ttaaaccact gccacgcagc ctgtaacttt
    23761 tcgcccagcc agtcaaacac cggtttcagt ggcgtgaaca gctcccccac cggcgcaaat
    23821 gccgctttca gcccttcaac cacaccgcca aagaatgcgc tgacaggctc ccagtattta
    23881 cggataagca acgccccggc gacaatggcg gccaccacag ccacaaccgg ccagctaatc
    23941 gccccgatgg ccgtcataac ggcactacca accgtcgtga agattgcccc cattgcgcct
    24001 gctgccgcga tgatggcatt gatgccggtg ataaccggcc aggctacaag accaattgca
    24061 ccgatgatgc cagtcagcgc cagcgcgcca ccggcaatga ggccaatggt tgacgccagt
    24121 gatttgtttt tctgtaccca gccgtcgagt tttaacacat actttgtggc cgtctgcgtg
    24181 agcttacgta gcgcgccttc ctgctggtca aacaggtctg tccccaccgc ctcataagcg
    24241 gactgaaact ccttaaagtc accgccgagg ttgtcctgca tgatatttac cagctctgcg
    24301 gtcttcccgt ctgaggcttt aaacgcagcg gtcagtttgt ccagctttcc ggttgacgcg
    24361 gcagtcatca gcacagcggc ggctgagctg gcctcctccc cgaaaatggt tttcatgtat
    24421 tcggcctgct gggcagtacc gagccggttt ttctcaaagc tggcctgcat ttctttcaga
    24481 atggtaaata ctggtcgggt gtttccctta ctgtctgagg ttttcacgcc aagctctttg
    24541 agtgcatccc atgcttttcc agtcggtgcc tgcaggcgac ttaacacggc acggcttccc
    24601 gtccccgcca ttgagcctgt gatttttgca tcatgcagcg ccccgaccat tgcggcggtt
    24661 tcttcaatgc tgacaccggc attttttgcc actggtgcgg cataggtcag cgcatcgctc
    24721 atgccgtcaa aatcagcggc ggttttgttc atcgtcatgg agagaacatc cccgatatga
    24781 gcgaccttat cgtttgaaag ctgaaaggcg gatttcatcc ccatcagcag ggcggcgttt
    24841 tcttccattg tgcggcggtt cgccagcgcc atattcagcg tgaccggcgt tgccgcctga
    24901 atggcatcaa catccccacc cgctttcgca atgataatct gtgcaccggc cgcatcatcc
    24961 gctgaggcgg cggtgttgtc accgagctgg cgcgcctgct tgcggagtgc agccatttcg
    25021 gcggagtctt ttgccacacc gagcacagcc tgcaattctg agtttttctg cgcaaactca
    25081 taacctggca tcagcagctt aactccggct atcgttcccg cagcagcaat ccccacaccg
    25141 gcagcgccca ctgaggccat atttccggcc agttcctttc cggcctgata acgctgtttg
    25201 actgcgttaa gttttgcctg ttgcgcactg acacgcgcca gcgcgtcacg ctgccggtta
    25261 agctgggcgg tggtttcact gatacggttt ttcagtccct gctcatcatg tgcaagattg
    25321 cgggtattaa ttcccacagc ggccagttcc cgctgctggc gtttaacgga atctgtcagg
    25381 cggttatatt tcgcctgtaa gtcctccgcc gcacgctttg cggattccag cactttcgcc
    25441 tgagcacggg tcgggcgttc ggtgttttta aactgtgtgg caagggcttc ggcctcctgc
    25501 cgtgcctttt caagtgcatg accagtcacg gcgagctgtg cactggtctt gcggaatccc
    25561 tcaatacggg atgcgtgacc gttcagctcg cgcagtgatt tttgtgtttc ccggatatcc
    25621 cccgacagca acttactcgc tctgcggatg gatttaaacg ggcgggacgc ctggtcaaca
    25681 gccctgagca atacctgtaa ttttacattg ttactcattc gtgtttccgc ttcgcctgag
    25741 cgccttttcg cgccatgtga tgagttcggt caggctcatg ggatacagtt ctgatggcgg
    25801 ccagtgaaat atcactgcca catccgccat caggtcatcg accgacagat ttttcggaag
    25861 cgtcactgca ccgagttcgg cgacaaaaaa ccgaccacct taccggccag cgccacaagg
    25921 tcaggcagtt ccagcgcggc gacttcctgc tcggtcagca tcggtgccgt catgcgcggc
    25981 agcaccttaa tcagtgcatc gacttctgag tttgcgaccg cagccagact gacaccgcgc
    26041 agcgtccccg cactgggttt catcagcgtg acctgttcga taacctgctc accacgcttg
    26101 accggattgt ccagggtaat cacattttct ttgttcatgg ttttctcact tctgaatcgg
    26161 ggttaaccgg tcagccaggc tgaccggatg aaaattacag gccgatattg cggcggtgtt
    26221 gctccagccg gtcgacgccg ttcaccttct caatcatgtt gatggtgtcg atttcgacca
    26281 gctccttacc gtccatcgtc agccggaaat aggtgcagac cacggagatt ttcgactcgg
    26341 tgtcttctcc ctgtttaccc tcgccggtgt cgatttcttt ctgacggcca cgcatgacca
    26401 cctctacggc caccgtttcg ccggtatcgt cacgctggta agagcctgca aaacgaatcg
    26461 gcacggcatc cacaccggtt gcggcgtaaa gctcccagat aaccgaatcc gggaagcccc
    26521 cgagcgacca ctccattgac agcgcatcgt catcaaggcc gaggtctacc ggtgcgcttc
    26581 cgttcatccc cgcaccgcga tagttttcga gtttacgggt cagttttggc agcgtgacgg
    26641 actttgcaac gccctgatag ctgtagccgt tcagaaagac gttcattaac ttgagtttgc
    26701 gcggcattgc catcggtcag gctccttaat tgctgttaac cgaggtgacc agatttgcca
    26761 ggtatttatc ggtaatacgc tggcgcaggg tcaggttttc aagaggaggc accggggtat
    26821 agtcgtagtc gatatacagt tttccggcct tgagggtttc cgcatcgttg gattcttcgc
    26881 tgaaccagca ggtcgcatcc acgatatagc cgtttgtttt cagctcacgg aatttggcat
    26941 tgatgccgtc aacgatgtcg cgaatcagcg ttgcggtgat gggcttgtcc accgcccaca
    27001 tgtgcgcctc agccatcgtg tcggccagca cctgcgcggt gcgggtgtag ttttcaaaga
    27061 ggaacagcgg gtcatcagag caggtacggt taccccagaa gcggaaaccg tcacggcgaa
    27121 tcagcgttgt gacgcctgac tcgttaagca ggtcagcatc ggtgccggac tcctgcaaat
    27181 cccagaatac ggacgcgctg atgccggtaa caccgttcac cccgacgttg gacagcgttt
    27241 tatgccagcc ctgctcctgg tcgattttag cgcgcagacc cagcgcacgg gcggtggcat
    27301 acgcggtggc ggtggtactg gcgaccgtat cccatgcgag gaaatccggc cagatgacca
    27361 tcagctcacg ctggctgaaa ttctgacggt aggctttcac ctcggaaacg gtcttacagc
    27421 cccatgcgct gatatacccg aaagcgcgca gcttctgaca gactgatgcc agtgcaacag
    27481 ccacctcttt ggtgtccagt cccggcacgc cgagaatacg cggtttaaca ccggttaccg
    27541 actccgccgc cagcagggct ttcagtccgg tgtactgacc gttttcgtcg gtggtgccga
    27601 tgatattgga aacggtctgc gcgagtttcg tttcctcgtc gtcgccggtg ccgtcttcca
    27661 cacgcacgac aacggtgacc ggttttgact ggtcggcgat ggcctgcaac gatgccgcca
    27721 gcgtgccttt tttaccggcc tttgcaattg cgctctgcac attggtaatc agcaccggtt
    27781 tattgagggg gaaggtttcc gcatccgcat cgctggccgt gcagaccatg ccgacaatgg
    27841 cagtggatac ggtggaaatg acgcgggtgc cgtcgttaat ctccagcacc tgcacgccgt
    27901 gatgatagtc actcatccgt ttaactccgt ggttaatggg tgcaactatt ttctgttggg
    27961 cagtgcatga gacgctattt gacctggctg gtcagtggat gaaacaacag ataaagaaaa
    28021 tgcgggcaat tcgcccgcat gtcctgattt gtacacactc attttccgac tgacagttta
    28081 catagcccaa acgctatcaa atctgacagc ctgctatgag cgaaaagcgt taaatctcac
    28141 gatatctgct accgggaaaa atttcgatta tcttcgatgg gaactttgat aatgtagtaa
    28201 atgccatttt cagcgatcac ttttcaagcg tgtgtaaatg attaatgtta aaaaattgac
    28261 ttcactgaaa taaggttgct agtatttttg gagcaaccat taatgttttt tatttaagga
    28321 atgtaaatga catacgcact gattgataat tcaactctaa ctgcagtcca aagaatcgag
    28381 gggaaagtaa aaacaagatc gaaggactca gtagatacag acataatagc atttgagaac
    28441 tatatacagt ctattttgtt ctatgatcac gttatcgcgg ttgatgatta tattccagag
    28501 taccgtggga gtaggatttc aagtttcaga gagatattat ttcttgataa agcacaatat
    28561 gggttggatg aaattgaaag tgaagcaaaa aaaatatctg atgcattgaa acctaaaatt
    28621 cgtggtggaa catttgttaa tgacgatttt aaaaaactaa ttgaacttct tcagacacat
    28681 atcatatgta catgggacat tagttcaagt gtttatcatc taacactgaa gaacttatct
    28741 gatggaggta aggagtttga aaaatatggt aacattgctg ctgccatttt ttcagaactg
    28801 cgtgatgctg ctgagtctgg taatagaaca aatggcgatg ttgaacttct cgatcgattt
    28861 ggcaacccta ttcgcaaggg gtacaaagtt cccggagcaa aatgggggga tggcacatct
    28921 gctggtgaag catcagatgc aatcaaagca ttcgtggctt cgcttgtatg gctggctaat
    28981 cgggcagtat tttattctct cacagctaag taccttcaag cagatacttt tttatatcca
    29041 ttacgtcagg cgtatcaaca gacatattta agccaaaact gtcagtatgg ttttgactat
    29101 gctaaacata ttgtagaaaa cttttccact tcgttaagtc atgatctcat tgatattcat
    29161 tctggtggta aagctgtagc aacagcaact tcgcttcctt tattttcggc atggctcgca
    29221 aaagaaactg gtgatccaag ttgtattgtt gaagctgctt ataacataag ggacaacaac
    29281 gaatttgtaa gggcccgtga gaaaatgcga gaaataaagc gcttgtttga tgaaaatgaa
    29341 ttgcctcaag ctaataagtc agtaagagca ttgcttactg aagtatcgaa agaatcagat
    29401 tatattcgaa ctaagtatgg gctcaagact agacaaggtg tacctgtaac taagcttgta
    29461 catgtataca atactgttgc ggctattaat tcccttccga aactaccgac ctttaatttt
    29521 aaagttaaag ttcctgattt cttatatgat cttaaaaaac ccaaggggtt tcgtgctctc
    29581 tatcgaaatt tgactaatga tctttctact gtgtggtcgt taggagaagc tcgagatatt
    29641 cttggaagta aagttgtgat agataatcat gcaagaattt acagtccgaa gcatgaagaa
    29701 ccgcgtttca aaaactctca ttccccatat aagagcccga tgtagcttaa gaaaaagaca
    29761 cgcccacctt gtgcgtctac ttctggcaca gagcggactc tcagattagg ctttactctg
    29821 tgccatagat atgtaagctc acaccagagc tcatacaact tattgcggca tttccggcca
    29881 ttcaggattt gcaggatcca cacgactgac cagaacactg tagcgttccc atgcctccag
    29941 tcgactgcgc tcctcatctg ttgccatatt cagcctgaca gcgcgctcca gcggcaaaat
    30001 cacggattca gcatctgcaa gaagtctggc tttccgattt tctgcctgct gctgcaattc
    30061 ctctgccgta taaattcgtt taatcactgt acagtcctta aacatccagt tccctgaaat
    30121 gtccgcccgt cggttagcag taatatccgg cacttcaaca acacttaatc catccggtct
    30181 gatagctgtc acatcctttt ccacatagcg gataatatta tctttgtcgt acgctatttt
    30241 tatcgtgtca tcagcaaaat acttttgttc ttcgtaccag ttcttaccat cttctgaaaa
    30301 aaaccagaca acatcaaagt cctttgtcaa ttgatattgt tcaaccgttt ttggattacc
    30361 tgccgttata tttatcaaat gctgcataaa ttatacctgt gccacgttat accaagtccc
    30421 gttaatgtat ttctgcaccg gtctgtaata tacgccacca atgttatcgg cagagtttga
    30481 gccggtatcc tgaacaataa tgccggaata tacacacccg gacggtgcct gatgtgtcca
    30541 tgtcatacca ttgttcgcag gtttgtatgt ggcagcacca ccaagccgga tatcccggac
    30601 atagcgtgaa tcaaagttgc catagttaga tggtgatacc tgcccgttaa cagcaaaagt
    30661 gatgctgtta tctgtatttc tctgactgta aaaatgccag cctgcatcat cacctaattc
    30721 agccaccaca ggacgacttg agtttcccca caaattgaat gcggcttcct tcgtggatgt
    30781 attgctgctg ctgaccgtga actttttccc gctaccggca cgtactttgg tacttgagac
    30841 aatatcacct gtaacactca ggccatgccc cattgacact ccgccattgg cgttattgat
    30901 agtcagcggt cttaagccgt tccatgtccc aaatttatcg ccagaggccg tcagcattaa
    30961 atatgtgctg ccgccatcat tcctgataaa gaatccataa ttgccataag caatgcgcag
    31021 accattagca ctgagtgatg taatttcacc tcttgaacgg agaccataag cggagctgag
    31081 tgataattct tcctgagcgt cattgtttcc tgtcgcccag cgaattaccc cgccctgaac
    31141 tgtttcatgc cagatagtat ctccttctcc accacgaaac tttctgagat attttttgcc
    31201 gcctctggtg cctgaacata aggccgtaga catataggca ttctggcttc cgccatcctg
    31261 attaatcgtt ccggtcattg agtcgccctg acgattccag tcacgacgcc agccggggga
    31321 gtagccgtcc ccatgattta tgtaagtgaa ttgcgcgctg gttgtaccgc caccgcttga
    31381 tgttgttggc gtggtcactc ggatagtgat tgcagattta gttcccatga cctcgacgac
    31441 acaaccagcc aggtggatat caccacatcc ggtatccgta atgattttgt tatttgcata
    31501 tgaccaggag cctttgcaca tccagtacgg atgattaaat gcaccacggg aatccagcca
    31561 ttcaataaac tgagcggttg tccagtttcc ggcttcagtg ctcaaagcgc cgctataagc
    31621 acgacaggca ccgatatttt tcgtgaaggt atcctttccc ggaatatccg caccgttctg
    31681 atctttctga agacgttttt cagcattgtc ataggcagac ttcactgctt ttggcgttgc
    31741 ggccagcgtt tcagaatcgc tgttgatggc actacttagc tggacaagac cttttcgcgc
    31801 tgtggtggca tcctgtgcag tgtatttccc gttagcaagg tcatacgctg tcttaaccgc
    31861 ctttggtgtt gccgcaagcg tttcagaatc gctgttggtg gcgctactga gctggacaag
    31921 accttttcgc gctgtagtgg cgtcctgtgc agtgtatttc ccgttagcaa ggtcatacgc
    31981 tgtcttaacc gcctttggcg ttgccgcaag cgtttcagac gtgctgttgg tggcactact
    32041 gagctggaca agaccttttc gagctgtggt agcgtcctgt gcggtatatt tcccgttagc
    32101 aaggtcatag gcggccttta ccgctttcgg cgttgcggcc agtgtttcag acgtgctgtt
    32161 ggtcgcactg cttaactgag taaaaccttt tgcggtcagc gaggcgtccg ggtgacgtcg
    32221 tgactgttcg tgctctgcaa ttttgtcatc aacgtaatcc tgtgtcgcca tcaccgttgt
    32281 ggtgtcaatg gtcagctcca ccgaggccac actgctgacg atgatgacca tgcgacaggt
    32341 ctgcgaacgc cctgagcctt cggcaagagc tggcttataa ctttcggcca tgttcgccac
    32401 ggcaattaac gttcccgcat catcgtacag gccaagctca cgcatccaga aaccgcccac
    32461 ctccggcgga ataaccagct ctgcgataat ataattactg tttcgtttgt cctggctgat
    32521 tttgttcagc gtatgtcgcc agacttcgtg gataagcccg gtctgtccgg catccgggac
    32581 aggcaattta ccaccgccat ccccgacggc catcgtggta atgttgacct tccgccctcc
    32641 cggtgcggtt gccgctgcca gctttgctgc accggcagtg gtgataacgg ttctgaattt
    32701 tgtgctcatt attcctcact tatccggggt aaaccgtaat tacatcgccg tcgtaagcca
    32761 caccaccggc gaacaggtag ccgggaatgt cccgggtaat gttcaggcca ataagatgac
    32821 ggcttgcagg tttggcatca gcaataagcc gttccatttc ctgatacatt gcctctgtga
    32881 tgccgctttc cagtacacca atatcaagcc ggaaggtgcc gggcgggtca ctgttttccc
    32941 accactccgt cacgttgatg agatagccga gcggctccac cacacgccgg attgcgccga
    33001 tagtgccttt atgacagtgg atgaaatagg catcgcggat aacggcgcgt tttgtcgctt
    33061 ccggccactt ttcatcccac ctgtcgaccg aaaacgccca cgccagccac ggcagcagat
    33121 ttgccggaca ggtatccggg ttccacagtt cacgaatcct gaccggcgtt ttttcaattt
    33181 ccgcacaggc ttttgcggcg gcaacttcaa gcggtgatga gccggtcggc agcagtcgcg
    33241 aatcactcat ccgagcctcc ggtcacgacg cggtattcgg tacagaaaga cgcctgcgta
    33301 ctgttgagca cgatgtcggc cagcggtgca gtcagttcga cacgctgcac gccttccaca
    33361 tgcaaagcgg cataaatggc agacagacgg atgtcgcgcc ccagccggtg ctgtgccgtg
    33421 atgtacgctt ccagtttttt cacggcagca gcgcgtatgg gttcgctttc gggaccaggg
    33481 taaaggtaaa gcgtggcgtt tatctggtat tcaacgatgg cggcagactg cacggtcacg
    33541 cggtcggcca ccggcctgac gtcctcacca ttaagggcgt tacgcaccac cgccagcagg
    33601 tcttcggatg cgacaccgtt attttcacgt gacagcacag agatggtgac gcaggccgga
    33661 gacggactgg ttacagaaat atccgcgaca cgcccgtcgg cactgcgacc atgatactga
    33721 taggccccca ccgacccggc gacgcttaaa ccttcaaacg cctgctgaat acgcagacga
    33781 taatccgtgt cagattccat cactgccggt gtcggcggga gggtcgaatc atctgccggg
    33841 gtgataatca ggcgcgtggt gttgtaattg gcaccaatca catcaaggtc attaccggcg
    33901 gcacaggcca gcattaccgc ccgtgcggcc tcattcacac gctgacgcca gataagctca
    33961 cgataagcat tttcctccag cagtttgacg agaggctcgg attccagcgt cagggtacgg
    34021 gcgaccgcct cctgctggtc ttccgggtaa agggaaatca gtgtcgcctt gcgttcggca
    34081 agaatggttt caaagtccag ctcctcgacc acatccggtg cgggtagctg gttcaggtcg
    34141 ataatcggca tggtttcaac tcacagggat ggttaacgaa agtggctggc cggtgtcgtt
    34201 gtgctggccg gttaacgtga ccgtcattcg cccgtcaaag ctgcgcgccg tggtgacgga
    34261 tgacagggtg acgcgggctt cccatttcag cacggccatg taacaggcga ccttaatctg
    34321 caactcaagc gccggggtct gcggctggtc aatcattgac gccagcaacg agccgtaatc
    34381 acgacgcatc acccgtgagc cgaccggcgt gcgcaggata tcgccaatac tctggctgat
    34441 atgctcaagg tcagagacag tcaggccatc actgcgattc attccgagat aacgcgctgt
    34501 catagaggac tcccggttgt gccgccgctg tcgccggggt gtttatgggt atgcagtacc
    34561 ttcccgtttg atgagagttc accgccggtg tgttcaatgt tgccgcgcat cgtcccgccc
    34621 ttctgcactt ccagcgtgcc agtgatcagc ttgtttgtgc agaccacctc cggcgtgtcc
    34681 agagtgacgc gggttgatgc tttcaccgtg accaccggca ccgtggcagt aacagaatca
    34741 gaagccgtca cgctggccgt tttaattccg cttaccgtga gtgcactggt ttcaggttca
    34801 tactcaatca ccgccccgtc agggaaacgg atatgcaggg catccgccga cgcagacggc
    34861 gcggggttat caccggaata aatccccggc agaacgaacg ccgtgtcgag ttcaccgcct
    34921 acggccagaa tcagcacctg ttcccccacg gaaggtgccc accatgtgcg cgaacgtccg
    34981 gcgcgatggg tcagccactg aagccagtcg gtgcacatgc cgccggtctg cacacggcag
    35041 cgaccggcat taaggtcggt ttcgacgata atgccggtgc ggatcatgtt gcgcagtgcg
    35101 cgcgcgagtt cctgaatatt tgcgagagtg ttcataacgg gaaggatgcc gccgggtcat
    35161 accggcggca atgtgacgat gaggtgtcag gaatggcaca actaacggtc gaggtgagcc
    35221 aggataatct cttcaatcat ctgcacatcc tcaccggtaa agccgagcag gggacgcgcc
    35281 ggataatgaa ttttcttacc gtctttccgg gtttcttccg acagaccgaa ctgatgcaca
    35341 ctggctattt tcggtgactt cccgccgtaa aactccattg atgcctgttc agggctggcg
    35401 cggatatgca aaaaacgact ggtgataagt ttcgcaaaca tttttcgctt aacgcgaccg
    35461 gtcttttttc tggcgctctg ctgctggcgt ggtgcgtagg gtgtgccgtc cggggctttc
    35521 tgtgccatca cccgacgctg ctgactctga cgcagacgtt tcgccagttc ggcgctcagt
    35581 cgccgacgcc ctgacggtga cagcgactca ataagtccgg tcagcctgtc ttcaaaacgc
    35641 ttaaactcat tcatcccact tactcaccag ttcgccattg atataaagct ccatcgggcg
    35701 ggtgaccggc tccggcggcg gaggttccgg gatattcttc acatgcagcg cgccgtccac
    35761 ctcactgacc agcgtgcgct cggtcagcat caggctgatg ctgatatcaa agctgctgtc
    35821 attgttgatg tctgcataaa acgtgaagcc ctttttctgg ccttcgtcgg tggtcatgat
    35881 gtcgggctga ttttcccgca gccacgccag caccggcacg atgagcaggt caaaatcacc
    35941 ggtaaagtcg gtcacaatca cgttgagcgt gtaacgcttt tcgaatgaca gcgacgtcgc
    36001 cagtgtggag gcaatactcc cgttatccac gaatatccgc agcatctcgg ggcttgtttt
    36061 cagcaccgtg acggcatcag tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt
    36121 cgtcctgaca gtgtttaatc atttttacct ggctggcaca gcgtgccagc gcgttctcaa
    36181 gctgccggat atcagcactt aaatcgccgt tcgtctgcgg gtcactgccc ggcatcgggc
    36241 aaaggctcac tttcgggcag gcgttgtgga caatcactgg cgtctgcgca ggctgtgcgc
    36301 tggtgcaacc ggcgcacagc atcaggcagg tcagcaccgt accagcggcg aaaatcttcg
    36361 ttttcattaa gtaacctcgt gatggttttc tcgcgctgag cttcacgctt cgcggcgttc
    36421 tccagttcct gacgcagtgc cacctgcgcc agctcgtttt tgtctgccct ggtgagggca
    36481 acatgaagct ggtttttcag catggtgatg gtcgtctgct gttcactggc gacgttgttc
    36541 gccctgtcca gcaaggcgcg caggctggca tttttgtgtt tcaccagaaa cagaccggcc
    36601 accgccagcg ataacaacac aaccagcaca atcatcagtt ttgacatggt tcccgcccct
    36661 caagacgctg acggcaggcc gtacgtatca gccggaaaaa cagcgatgcc acgagataaa
    36721 tcagtgcggt aaaaatccac ccggcagcga ccagcgagat aaacgtcgcc accatcacca
    36781 tcagagccgc tgcccgtctg cgccacggca ccggctgcaa aaacagcgac gtgacaatct
    36841 tcacggccag cgattccggc ggcagctccc gcccgtagcg ttccagcaca tactccgcgg
    36901 catacacgcc gacaccaccg gcaatcacac agataaccgt cgccagaatc gcccatgcag
    36961 cgacaaaact gacggccacg ctctgcgggt aaatcaggga cagtgccagc atcagcgcca
    37021 gcgacacgtt cagcatcagt gaaagggata atttcttcat ggtgtttact ccgtttaagc
    37081 cggtacgccg ccagcggtgc gccagacggt gaccagtttt tccagtgaat gctcacgctg
    37141 accgtaaccg gcacccggca gggacgccca gatattgcga cagcgtgaaa tggcgcgctc
    37201 aatgcgtccc gcccggatgt catccagtgc accgcgttcg cggatcaact gaatggcgag
    37261 cctgtcctgt gacaacggac tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca
    37321 gaacaggtaa agctgctgat agcgaccgga ggccgtggat ttttcaccgc gacggttaaa
    37381 caccttcgcc ggtcggccat gtgcgaacgg gtggtcactg tagtcggtga agatttccgg
    37441 cttcccgtcc agtccggtga ctatcacgtc atagccccgg tttttcgtca gcggatgatt
    37501 cgccgtccct tcggacacgg ccagcatgtc gagaaaggcg gcgatattct gatgtgtgct
    37561 aattaccggc attacggttt ccccctgccc ttaaagcggc gctgaatggc aatctcaatc
    37621 acctgataac cggcgatacc cagcatggag ccgatgccgc acaccgcagg cagtgacagg
    37681 tcaggaaact gcaccagaac aacaccggca accatcgaga caaaaccacc gagcaacatg
    37741 cgcccgataa acagacgcgg agtgatgggt tcaccaccgg caagcacctt gccgacaaca
    37801 atcagcaccc caatcatgaa aagcgacagg acgctttttt cttctgctgt catgcgttac
    37861 tcccacagat tgacagtttc agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg
    37921 gtgccgtgtg gcagcaccgc ccccagttca gccagtcccg gatttgcggc gagcacggcc
    37981 tcgaccacgc cctcagtgcg cccgtaatac cgaacacaaa tggcgtcgag cgtgtcgccc
    38041 tgtagcgcaa aggtcttcat cagatttgac tcacgatgca gcgcggcttg tcctggatac
    38101 gcgccaccgc ccagcgcata tcccgccaca gctcatcaat ggtgctgtca atgctgtcgg
    38161 ccttcttgtc gcccttggca ctggcatcca cgccgcggta acgctcataa agcgacgcgg
    38221 tcgccatcgc acacacagcg cgctcgtagt aaaaaacttt gatgctttca ccgtcgatgt
    38281 cgtccgccgg gacgtcagcc agacgcgaaa aaccggcagc aattttctgt tcgcggtact
    38341 cgtacagctc cgcattcgtc tccgccatgc ctgacttgat ggcctcacgc agacgggcgg
    38401 gggcgacggt ctgctcaagg cgcatacgtt cccggacgcg cttcgggtca atatcgggaa
    38461 aaaagaacgt gtttttaatc accggctcgt cgcctgccgg ttgcgggata accaccgtac
    38521 cctcaccgga cacgggagcc tcctttcgcg gaataatcag cgtcatcatg actacctctg
    38581 aaaagtcggg cggtggacgc cggtgcagtg tcaggtgatt caccgtcact gaccggcgtg
    38641 ccgccctggc gcggggcgca ttcggttgtt aactggcttt ctttttcggg cgtccacgtt
    38701 ttgccggtgt cacgctccgg gtcttacgcg gagtgcgggt ggccgctttg ggctgcggct
    38761 ccggcttcgg tttcagctcc cgctccagtc gttcaatctc ttttttgacg cctgcctgac
    38821 agtcgagctg tgtcgcacgt tgcaggtgta ccagcgcacc tgcggcatca ccagcgtcac
    38881 gcagaaacag accggtgatt ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg
    38941 tggcggtcag ttcgagggtg tccgtcagca ggcggggatc cacagactca ccggcagcgt
    39001 gagcgcgcat ggccgcaagc gccacctcct cggtgaacat gtacggcggg gtgcggcggt
    39061 gtttacccgg catggtcaga ccgtacttca gggcataacg ggcaatctcc agcgcaccgg
    39121 caatatcgcc ggtatccaga cgccacagca tgaccgtcat cagaatgtca tcctgtgcac
    39181 ctttgccctg ctccagcacg ccgttcaccc acggcaacca gaacggcagc agttcgcgtt
    39241 ttttcgcggc cttcagctct tttgaataaa tcgctttcag tgtgcgctgg tctgcggcca
    39301 gcttgaccag catctgctca tagacagttg catgtcgcag cggggcggct tcccgctgcg
    39361 cggtcatcgc tgccgagacc cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg
    39421 ctcccggctc tgcggtcgct ttagccggtg tggagaaatc accgacctta attttttcca
    39481 ccagacaacc ggcggcgtag tcctccacca cgtaatcaat gttcattgac tcgtagttct
    39541 ccacgcggtc gagtttcggg ttttccacaa tcacgcggcg atggctgtca tccatgtagt
    39601 agatggacag gttttccagc ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc
    39661 gtaccgccgg caggttaccg atgcgtttct ggctgatgat gacgtcagcg gccagcatct
    39721 cgctgttatc ctgctccttg ttgacgatag ggaaatactt gtccgccagt agctgacgcc
    39781 ccacaatcac cacaaggtca gggtcttcct gataccaagg ttcaatcagg ttgttggtcg
    39841 catccatcac cagtgcatcg aggcttacat aatcaccgcc cttacccacg cggataacct
    39901 cagaggtggt gcggccttcc tcgtcagtga ccttgctcat cacgcgcgcc ggggcttcat
    39961 tcggtatttc tgcagccagc cgaccgccac atcctgcagc atcggattac tgttgcggtc
    40021 agaggtttcg gcacgcttca cgccgttaaa accggccatg atgaaatcaa gggactggcg
    40081 tttgataatg gcgttacgga tacggagctg gaaatcctga taacgcgccc acaggtcaag
    40141 cgttttgtag cggatataaa aatcgaagtt aacctggtcg cattcgtact tgtttgacgc
    40201 cagcttcgag aagtccttcg gctgacgctc ggtgccaccg gcggtgtcgg tggtgctggc
    40261 gatggagccg gtgacaccga tgccaatttt ttcccctttc atttcactga ccggcacaat
    40321 gttgatgcgg gtcagaaagt cagaggactc ctgcatggtg ttcatcaggg tctgggtgac
    40381 cgacggttca acggtgaatt ttttcgacac atcaccggcg tcgatgccgt tcagctcggc
    40441 aacacgggac aggtaagcat taaatttaaa gcgggtttcc tggcgcatag tttttcctga
    40501 aattaagggt taatcgtgaa ggttttcccg gactgactga cgccggtcag cagttcgtca
    40561 tcagggcgtc accgccacca ccggtggcct tgctgcggcg ctgctgggtc agactttcgg
    40621 tgtggtcgag actgtttttc aggcgggtga atgcctggct ggtttcatcc gccctgtcag
    40681 tcacctcctg cttaagtgca gaaaaggcgg tttccatctc agcgaggcgc tgctcagtgg
    40741 cgctcagttt ttcctgcaca tgttcagcaa cagcggtcac cgcttcatgc acgtcattca
    40801 gacgggcatc atcgctggcc tgtttgcggc caaaaatgga tttcaccttt tcggtcaggg
    40861 cggtgaacac ggtttcaggc aggtcttcaa attccagctc aacaggcgtt gccactgaaa
    40921 tcaggttttc agggcttaat ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga
    40981 attccaggta ttccgtgccg aggcttgccg ggtcatcggt gacggccaga cccaccagat
    41041 aacatttgcc ggtgttggca aagttcggct gaatttccat tgaggtgtag accttctgcg
    41101 cggccttgtt catcgcgata aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt
    41161 tgcctttcag cgccgaatca tcgtcaatct tttcggcctt cagttcggcc acatcgccat
    41221 aacgcttaaa aataccgtca ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac
    41281 catagacacg cgggtcaaag gtttcggcca tttcctgaat atcctgcgca ctgatgacac
    41341 gcccgtcaca ggtgtcaccc tcaacgccga tacgaaagaa ttttgagact ttttttgcca
    41401 ttgtcaggag tcctgaatag tgattagagg agtcacatgt cggcatcagt ttcccgacga
    41461 tgcgcatcct ccgccatcag tcccggatgg tttatcactg acacaacagc acattagcga
    41521 atcgcggggc gcgactcagt agccttgccg tgtattcatc acggcgaggt attcatgacc
    41581 atcaccacag acaccactct tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg
    41641 caggggtttt ccgtgccgca gattgccgcc atgttgcaga tgaaacgccc gacggtgcag
    41701 agctggaaac agcgcgacgg ctgggacagt gttgccccca tcagccgtgt cgaaatgagt
    41761 ctggaagcgc ggctgaccca gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag
    41821 gaaattgacc tgctcggacg ccagattgaa cgactggcac gggtaaaccg ctacagccag
    41881 accggcaacg aggcagacct taatccgaac atcgctaacc gcaacaaagg cgggcgtcgc
    41941 aaaccgaaaa agaatttttt cagtgacgag gccatcgaaa agctggagca gattttcttt
    42001 gagcagtctt tcgactatca gttgcactgg tatcgtgccg ggcttgagca ccgcatccgc
    42061 gatatcctga aatcccgcca gattggcgcg acgttttatt tttcccgcga ggcgctgctg
    42121 cgcgccctga aaaccggcca taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat
    42181 gtgttccgtg aatacatcat cgcctttgct cgtctggttg acgttgacct gaccggtgac
    42241 ccgattgtcc tgggcaataa cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc
    42301 gcacagagcc ataacggcga cctgtacgtc gacgagattt tctggatccc gaattttcag
    42361 gtactgcgta aggtggcatc aggtatggcc tcacagagtc acctgcgctc gacctatttc
    42421 tccaccccgt ccacgctggc gcacgacgcc tacccgttct ggtcgggtga actgttcaac
    42481 cggggacgcg ccagcgccgc cgaacgtgtg gaaatcgacg tcagtcataa cgcccttgcc
    42541 ggtggtcttc tctgtgcgga cggccagtgg cggcagattg tcaccattga ggacgccctg
    42601 aaaggcggct gcacattgtt cgacattgag cagctcaaac gcgaaaacag cgccgacgat
    42661 tttaaaaacc tgttcatgtg tgaatttgtt gacgacaagg tatcggtgtt cccgttcgag
    42721 gagctgcaac gctgcatggt cgacacgctg gaagaatggg aagactacgc gccgtttgcc
    42781 gcaaatccgt tcggctcccg cccggtatgg attggttacg acccgtcaca ccgtggcgac
    42841 agcgccggat gcgtggtgct ggcaccgccg gtggtggccg gtggcaaatt cagaatactt
    42901 gagcgtcacc agtggaaagg catggacttt gccacccagg cggagtccat ccgcaaactc
    42961 accgaaaaat acaacgtcga atacatcggt attgatgcca ccggcctcgg tgtcggcgtg
    43021 ttccagctcg tgcgctcgtt ctatcccgcc gcgcgcgata tccgctacac gccggaaatg
    43081 aaaaccgcaa tggtgctcaa ggccaaagac gtcatccgcc gtggctgtct ggaatatgac
    43141 gtcagcgcca ccgacatcac cagctcgttc atggctatcc gcaagaccat gaccagcagc
    43201 ggacgcagtg ccacctatga ggccagccgc agcgaggaag ccagccacgc cgacctcgcc
    43261 tgggcaacca tgcacgccct gttaaatgag ccactcaccg ccggtatcag cactccgctg
    43321 acatccacca ttctggagtt ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg
    43381 cggcaaaaac aatcaccgcc agcgccccga aaatggaagc attcaccttt ggtgagccgg
    43441 tgccggtact cgaccgccgt gacattctgg attacgtcga gtgcatcagt aacggcagat
    43501 ggtatgagcc gccggtcagc tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc
    43561 acagctcacc gatttacgtc aaacgtaata ttctggcttc aacgtttatc ccgcacccgt
    43621 ggctttccca gcaggatttc agccgttttg tgctggattt tctggtgttc ggtaatgcgt
    43681 ttctggaaaa gcgttacagc accaccggta aggtcatcag actggaaacc tcaccggcca
    43741 aatatacccg tcgtggcgtg gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc
    43801 cgacagcctt cgcgcccggc tccgtgtttc acctgctgga gcctgatatt aatcaggagc
    43861 tgtacggcct gccggaatat ctcagcgccc ttaactctgc atggctgaat gagtcggcca
    43921 cgctgttccg ccgcaagtat tacgaaaacg gcgcacatgc cggatacatc atgtacgtca
    43981 ccgatgccgt gcaggatcgc aacgatatcg aaatgcttcg cgaaaacatg gttaagtcga
    44041 aaggccgcaa taactttaaa aatctgtttc tctatgcccc acaggggaaa gccgacggca
    44101 ttaaaattat cccgctcagt gaagtggcaa cgaaggacga tttttttaat atcaaaaaag
    44161 ccagcgccgc tgacctgctg gacgcgcacc gcatcccctt tcagttgatg gggggcaagc
    44221 cggagaacgt cgggtcgctg ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg
    44281 agcttatccc gttacaggac aggatccgcg agataaacgg ctggctcggt caggaggtca
    44341 tccgctttaa aaactactca ctggacactg acaacggctg aacatcgccg cctgcgggcg
    44401 gcttttttac accccgccat cacgccctca cacgctcatc accgcacaaa aaatcccgca
    44461 gacacaccaa cgcccaacgg gcagactaag cgccgtcacg acgcgctgag acgctggaaa
    44521 ataaaatcag caccaccgcc agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg
    44581 gggcggtttt aatgcagttg catgaacact ccaagagcac gccagcgcta gtgataacta
    44641 acaataaaag cgacttctga cgcatgcaaa tccatgcaca tgatggcatt tttcatgatc
    44701 tatgcggtat gctatccgct tcaattttta acacttgtta agggaactag acatggacat
    44761 tattgattta aaatctcaac tagaaaatga agataatgcc aagaaaagag cagagttgct
    44821 tgctcagcta gaggtgataa agcaaaacgc tgaagagcaa attcgtgctg agcaaaagga
    44881 tgtggatttt gagaccaaag aatttaccgt tgagttatta gttaataagt atcataacgg
    44941 attagaggat gatactaacg aactctttgt tcctgattat caaagggatt ttgtttggag
    45001 tgaaaaaaga caatcaaaat tgatagagtc tttgattttg ggttttccta taccatatat
    45061 atttatagca gatgttctct ccgaagatcc cgatttagat gggcgaattg agattgttga
    45121 tggctcacaa cgagttagga ctatacacgc ttttatccaa aatgaattag ttttacaaga
    45181 cctgaaatct ttagacgtcc ttaatggttt caaattccaa gatttaccat tatcacgtca
    45241 acgtcgtttc atgagaattc ctgtccgcgt tatagaattg agttctaaat gcaatgaaga
    45301 aacccgtaga gatctttttg agcgtattaa ctctggcagc gatatattga aagatatgga
    45361 agtccgtaaa ggatcagaac ttggttcaac aaatttatat acacaggtta ttaagccatg
    45421 ctcaacaata cctaaattta aagaattagc acctttatct cagactaaag aaaaacgaga
    45481 tgaacggtta gaatttacat taagattctt tgcctacgta gataagtaca agaactttga
    45541 tcattccgta cgagattttc taaatgacta tatgtcagaa aatcgagata tagacgaaac
    45601 taaacaaaac tcaatgaaac aagagtttgc taatgttcta tcttttgttg aaaactattt
    45661 cccagcagga ttcaagaaaa ctattactgc aaaatctaca cctcgagtta gattcgaatc
    45721 tctcgctgtt ggcgcagcac ttgcattacg tgaagtccct gaccttgttc ccaagaactt
    45781 agattggcta agctcagaag aattcaaacg attgaccact tccgacggag ctaatagtcg
    45841 cgtgaaggta attgaacgaa ttgaatatgt tcgggataaa ttattagagg attaacatgg
    45901 atcgttttcg tgaggatttt gacgaaaggt caggagagat cttggcctat ttggatcttt
    45961 tgaaatttat tgagtatgcc ggtgcagaac tgatatcagc tgatgataaa gaacataagt
    46021 tctccattac tgctcaatca cgaaaaactc taaagggagc agtatatata ctgttatata
    46081 acctcattga atcaacaatg agagaagcta tctgccttat tcatgagact atttatgata
    46141 gaaacgtaga atttgacaaa ctcaggaaaa atctcaggtc agaaat
//
LOCUS       DAZPBM010000013        46027 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPBM010000013 DAZPBM010000000
VERSION     DAZPBM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46027
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.013"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(69..929)
                     /locus_tag="ACHUZQ_000642"
     CDS             complement(69..929)
                     /locus_tag="ACHUZQ_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024427.1"
                     /translation="MKELQKINSIMERLIALELKLADCGKLCAEKQNELYREFVTIVS
                     ELMPIVNNCLMTSRFFNVDATTSRKFTVKIQERVSGSVTREMAEQFVAMSNKTKRKKA
                     LLVTVNQLSGRVDFQVRQGGRVLVNDAFVGRCFSPYSMKYDVDADDQDISISWKSETA
                     GIVLTAELLSDDKNDGQHHLDEKNHISEICMKPRSGMTLLSRTLAAGFSATEKRVLLV
                     VPEDADIRPLNGCDVISAKTLHTRIMPVPDVVIIDDIEKCKSVSSTATIKRILRCSTS
                     AVVFQKQLHN"
     gene            complement(1210..1521)
                     /locus_tag="ACHUZQ_000643"
     CDS             complement(1210..1521)
                     /locus_tag="ACHUZQ_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFR1024428.1"
                     /translation="MKIYIAGPMTGYENFNRDAFNKEADRLSRHGHSVLNPATLPNGL
                     TQREYMDICFAMLRCADAILMLPGWKASAGATAEYHYAYKMEMPVFTTLNYPPACSSA
                     V"
     gene            complement(1563..2030)
                     /locus_tag="ACHUZQ_000644"
     CDS             complement(1563..2030)
                     /locus_tag="ACHUZQ_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1024429.1"
                     /translation="MSRVTAIISALVIGIIVCLSWAVNHYRDNAIAYKEQRDKNAREL
                     KQATATITDMQQRQRAADALDAKYTKELANAKAENDALRRKLDNGGRVLVKGKCPVPA
                     SAKTSSASGMGNDATVELSSVAGRNVLGIRDGIIRDQTALRTLQEYINTQCLN"
     gene            complement(2027..2524)
                     /gene="rrrD"
                     /locus_tag="ACHUZQ_000645"
     CDS             complement(2027..2524)
                     /gene="rrrD"
                     /locus_tag="ACHUZQ_000645"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR1024430.1"
                     /translation="MQSSLRKAVTAAIGGGAIAIASMLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCYGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2524..2796)
                     /locus_tag="ACHUZQ_000646"
     CDS             complement(2524..2796)
                     /locus_tag="ACHUZQ_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024431.1"
                     /translation="MKMDERYSSASYGSAGLAAFFASLSLQDWGFIIGVAFSIILGVL
                     TYRLNKREQMKRTKILQDILDKTNTNNLSATAMVIGELGKRAPKEI"
     gene            complement(2892..3968)
                     /locus_tag="ACHUZQ_000647"
     CDS             complement(2892..3968)
                     /locus_tag="ACHUZQ_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1024432.1"
                     /translation="MFKTTNIHGAQLVCADSLQFIKTIPDNSVNLIATDPPYFGVKAN
                     AWDNQWDSDADFLGWIDEFLAEFWRILAPNGSLYMFTGSRRASKIELLTRDRFNVLNH
                     IIWAKPSGMWRGCHKESLRAYFPATERILFAEHYGASGYAKGQSGYASKCADLRKNIF
                     SPLIDAFSLARRQLDISAADINSATGKQMCSHWFSYSQWRLPSLIDFNKLCELFRRKA
                     DSLGVPCPYPFNVDYSEHEKRYSDLKLHYEEVKKQYDDLKAQYESLRRPFHVTADVPY
                     TDVWEFPPVQYYPGKHPCEKPAAMMEHIIKSSSRPGDIVADFFMGSGSTIKAALKLGR
                     QAIGIEIEGERYLQTVDEVKKLFE"
     gene            complement(4236..4463)
                     /locus_tag="ACHUZQ_000648"
     CDS             complement(4236..4463)
                     /locus_tag="ACHUZQ_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024433.1"
                     /translation="MNINEIRGNMTEAALSVECVMRGYPRISLKELSEACFLSQAAVE
                     FIIEQMICFGVAKRSGSGRYSLTDEYKQATF"
     gene            complement(4477..4698)
                     /locus_tag="ACHUZQ_000649"
     CDS             complement(4477..4698)
                     /locus_tag="ACHUZQ_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024434.1"
                     /translation="MNYQGNEKMRGDVAEIANELYELWQKVERFEKEYGFNSTNLTDR
                     LAGRLIGTMGPKLAELNRFMADVDFQFQD"
     gene            4862..5173
                     /locus_tag="ACHUZQ_000650"
     CDS             4862..5173
                     /locus_tag="ACHUZQ_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1024435.1"
                     /translation="MFNVITHPAALDELLELPDDLRGRMTRLIERLEKEGNKLKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPVKAIEIARTRLKEM
                     N"
     gene            5173..5460
                     /locus_tag="ACHUZQ_000651"
     CDS             5173..5460
                     /locus_tag="ACHUZQ_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1024436.1"
                     /translation="MKAKGIPFNEVKEKLLNTPEAIKGYEEADKELEMVEMLYEMRER
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLSKYAQACGATININAVY"
     gene            complement(5504..5707)
                     /locus_tag="ACHUZQ_000652"
     CDS             complement(5504..5707)
                     /locus_tag="ACHUZQ_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024437.1"
                     /translation="MTEASVSTLEELGSNLKDIDAVLDLVSVALASPEASLHLGEISR
                     LVSMSREIAQNCQQAIAVECLHH"
     gene            complement(5964..6290)
                     /locus_tag="ACHUZQ_000653"
     CDS             complement(5964..6290)
                     /locus_tag="ACHUZQ_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024438.1"
                     /translation="MALTAAERQRRRRERLKKEGTSRRDWILEPDELRMLGEICVLRR
                     PGRVPYSENEVIGLLIRKNYKELQKQLSTTCPRCGQKMPVSECIFDGEGSCHLTTTRL
                     KLVLKV"
     gene            complement(6306..6980)
                     /locus_tag="ACHUZQ_000654"
     CDS             complement(6306..6980)
                     /locus_tag="ACHUZQ_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1024439.1"
                     /translation="MKFSKFSELVNRILLNRILSNNHSDRRDMDVTIVVHSPGSIGST
                     PSVEVQSIHAGFDWDSGKVLIFPAQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHK
                     EQLEKLSIELESAKNHIAELEAKPVSQTYKLTFEQWLEQQHDKIDVDCGCVSTEMLMH
                     WIRVAYEAGNSPVTPDGHIQFSVSLPAACGGDKYFIDGVFQPLRYERDCEKAVVAAGG
                     VVNWVK"
     gene            complement(6977..7267)
                     /locus_tag="ACHUZQ_000655"
     CDS             complement(6977..7267)
                     /locus_tag="ACHUZQ_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFR1024440.1"
                     /translation="MNIDTTITIDTALNTGLALLGWFYIMFSAGKWAASVFLKQWGKR
                     RKQEKRQKALEAFYDAFELSRIEPGTTAKIATKGDLMIVMFRQERAEKGESA"
     gene            complement(7267..7686)
                     /locus_tag="ACHUZQ_000656"
     CDS             complement(7267..7686)
                     /locus_tag="ACHUZQ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1024441.1"
                     /translation="MAKAFTPEQREELNKQIVELVRLNGRGTVRQLADETGISRCAVS
                     RLSRELAASGDLYISGSGIFLSAQARKDWQNARKKLSRVKPKKSVVVDPDLIWSLPDG
                     EIRRYDRRLNMICHDCRKSEVMQRVLAFYQGNFQEVA"
     gene            complement(7686..8243)
                     /locus_tag="ACHUZQ_000657"
     CDS             complement(7686..8243)
                     /locus_tag="ACHUZQ_000657"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFR1024442.1"
                     /translation="MIDLETMGTNTCAPIVSIGAVFFSPESEELGPTFYVPVNLRSSM
                     LNGGIADGDTILWWLKQSKEARAAICTNDALDIKDALFELSHFITCHACNLKKLKVWG
                     NGATFDNVILRGAYERVGLACPWEYFNDQDVRTIVNLGQFIGFNPKKDMPFDGERHNA
                     LADAIHQAKYVSAIFSRLVKGQGES"
     gene            complement(8309..8482)
                     /locus_tag="ACHUZQ_000658"
     CDS             complement(8309..8482)
                     /locus_tag="ACHUZQ_000658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024443.1"
                     /translation="MTHDEAERLSDTYRRRGKKVLVVRSDFLGDGYCVYVHLPESERT
                     PKPSRTYQQKIWV"
     gene            complement(8746..9456)
                     /locus_tag="ACHUZQ_000659"
     CDS             complement(8746..9456)
                     /locus_tag="ACHUZQ_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR1024444.1"
                     /translation="MELEYIRSCVSTALADVHYRQRGILEVQLEQMRLGRSGRFNNKP
                     VRSISVGDDNSYEVSVPAEPVRFHQGKTFKQSSMLLTDIDFQSASWRRAIGQLNNEES
                     AWLYYCYGCKPDYNNDVIVCQWLWLDFLVAHSGGGFKKMKASTKKAMRKLIYYAAQQV
                     KSELTCAEAVDEREQDRHLSFLLNISIDSWRQDYKERWLLIKSRCLSLNRTALLNAAE
                     KRSEIIKRHRAGSAILPL"
     gene            complement(9446..9667)
                     /locus_tag="ACHUZQ_000660"
     CDS             complement(9446..9667)
                     /locus_tag="ACHUZQ_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1024445.1"
                     /translation="MKPEELIRHFGSVEKAAAGVGVTPGAVYQWLTAGEIPSLRQSDI
                     EVRTAYKLKSDFTTRRVKQSIKESRKRGA"
     gene            9759..10355
                     /locus_tag="ACHUZQ_000661"
     CDS             9759..10355
                     /locus_tag="ACHUZQ_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1024446.1"
                     /translation="MKTRGERLKARRLEMKLTLREVAEAVDISIAGVQNLERGDVMPS
                     LEIGIALAKCLRKPVHWVLYGTEYDPDRIPVIGTTESGPDEEWKPGQVPNTERFLPFI
                     SQRNTVYALTISNLTQGNYQPGDFLLVDSALELVPGEDVLVRDTAGNITIQRLARFDD
                     ENYYLDGTNSQRAIFRKSDLEFVHQIVGTIKSFMVEGR"
     gene            10601..14596
                     /locus_tag="ACHUZQ_000662"
     CDS             10601..14596
                     /locus_tag="ACHUZQ_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein, CyRepA1 family"
                     /protein_id="HFR1024447.1"
                     /translation="MTLNDFYADRFGSDPFSLIEAARDELTELAQMAGINWPACSDNI
                     QLNPRGGVERYSSYNKTSPEALEKSLKGRVEIYSRLEHSKGIDYPFINFVHKGSDAGS
                     WSGFSFLFSEYRREQQRNNATVVAQPEEERERIERQAEARRRREEQKRINDLKNNQMD
                     HERLLGWLAFHSAWEHAQAEDGSWPYAIKKGIRDVFSSCDVRRVTSHDSAKWSRGPTT
                     YMAIPLAHLDGRYDGRIVGWQRIDKQGGKYQTSAVTSGDFVGACFVIGELKGAQNIAV
                     TEGFATGASIWLATKKDKRFDAVVVAVSANNMVHVVEQLVNMYPAAKITCALDNDRKS
                     ATEGKGNTGLRTGYEILSKFNDVRCVYPTFEDDPQMECSDFNDLHKLRGLRETARQLF
                     ATANRLKTGTDLLSLTLNRLKTIKRDNRRTFAKELLRAVDIGMLTCPVPNSPSDLFNM
                     FCIVLREMGLENIYRATVKDHIARRLNKKCRIAQAPRSFSERITDPNKRPQHITYKRF
                     ETSVMTDDVLKYVQSLQGIVIVRAGMGSGKSTGLLRPLMHSSERGISVAHRVSLIGGL
                     WEMMTEGKGVKADILHYQDPGYQEMAPYASKLTICINSIVKNCWQPLMRQHDYFGFDE
                     ATQGLRAVLSGRAMENPVAVFNTLIDALARTEQHPIMVDADANDLLVDLAELAMKRRE
                     ELGLPAWLQIHVIELPVDVRNRETGEPIRVFYTEKDRIMTEVVKAVSDGEKIMLATDS
                     STFAEDVTATLRMHFPGKKFLCVNQKNKPEPEVEAFTNQPKKMVKKYDGLIYSPSISS
                     GVSIEQKHFDRHFGMFCGEVVPSDAVQMLRRDRTAKEFIIGFDKARAKRETDPQKIER
                     AFAQALLATAGMNGELTDVVFDGDRISMGVANTDFTRMKIKAAAIEATARNDYASNMI
                     CIMHSDGYKVSPLATDYAANDIGKDLRKEARDIVWEQTLDLHLNTETPQESEREELLK
                     KRALTLEEQAKLVRWDIENELKLPVEEGTLKFYFDGARDKVRRYETMLLDEVTARRYD
                     REEAAINFTYAYRQAGQWQYFVVTAMTREQADDVFKTKFPGIIDHKVKATPTVEVGAR
                     GFYALKSATLRQYFIDCGINPETMTGEATQERLKFARDNLMTAERRDLLNNVLHIGGF
                     MTPKGKPKVPEALFKNICDSLGLKTGKRRGRDGDKRPTLRFVEPESAAFMLDIIVQRQ
                     KDGLTLKTRKAEKTAIEMDRDLDLNIYMDDKTRSTNEQVQNAPHSVISEALAVLPVAV
                     PESWAMSALSSEELATMSSWSSASIAMTFASLYLTEFMDHLSSNELRRLREYITGNSS
                     CSYEEQGAFYG"
     gene            14785..15111
                     /locus_tag="ACHUZQ_000663"
     CDS             14785..15111
                     /locus_tag="ACHUZQ_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024448.1"
                     /translation="MSTTAFLDKLDYRQLQFCRDECERRIRAIQEEEKKVAWAVTDRG
                     LIFGWFRTEDYLKAVELLARKAAERWERADKENPETKYELNLYIEGNRLPLSEYNALF
                     ADGEWG"
     gene            15114..15509
                     /locus_tag="ACHUZQ_000664"
     CDS             15114..15509
                     /locus_tag="ACHUZQ_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024449.1"
                     /translation="MAVQSYKYESPLDKGYIRIKLSRYQHKAIFKVRKIRILDAYAYY
                     YNGENVVVEHFLARWFVALIFVPLLLIGTFVDGFPSTWREIKKGLFPRKYGRFSQDSW
                     RVIPGKHSVDEQPIVDFWLNRMADAKKVD"
     gene            15537..15824
                     /locus_tag="ACHUZQ_000665"
     CDS             15537..15824
                     /locus_tag="ACHUZQ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024450.1"
                     /translation="MKSFIFVLCFAFSAAVYAQEDSISVINSSADLACKNNPDAASKE
                     SCKELLLSTAAFASENAKFYSAFCRGYVKDEDKEKCDDAGKLVRYFQSLNQ"
     gene            16201..16365
                     /locus_tag="ACHUZQ_000666"
     CDS             16201..16365
                     /locus_tag="ACHUZQ_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR1024451.1"
                     /translation="MFKFKFAAIVRTDKKSHIHHLSTIASSEREARRQFASRFVLVLS
                     ARIPVREVAA"
     gene            16362..16595
                     /locus_tag="ACHUZQ_000667"
     CDS             16362..16595
                     /locus_tag="ACHUZQ_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024452.1"
                     /translation="MNQVKMNTVGLLESLDERLAQVYALVSSAHRTISSCEASIYLSE
                     AGKLLQIACELTHEAQGYSASLSSQLLHDKEAE"
     gene            16592..16756
                     /locus_tag="ACHUZQ_000668"
     CDS             16592..16756
                     /locus_tag="ACHUZQ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFR1024453.1"
                     /translation="MSALAVFSFQEEHQVRVVMINGEPWFVASDVCMAAGIDSTAIRK
                     LDEDEKGQAR"
     gene            complement(16802..18697)
                     /locus_tag="ACHUZQ_000669"
     CDS             complement(16802..18697)
                     /locus_tag="ACHUZQ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="telomere resolvase"
                     /protein_id="HFR1024454.1"
                     /translation="MSKVKIGELINSLVSDVESIDNSDRPQGEKTKKIKAVALKFKNA
                     LFNDKRKFRGKGLEKRITANTFNAYMSRARKRFDDKLHHSFEKNIHRLSEKYPLYNEE
                     LLSWLSLPAADIRQRMSALQAKLKLILPLSEDLSNIRIGDNKSHNKLLKLSNKYKEWQ
                     FAISDLTSDNWKDKRDYLYKLLQQGSALLEELNQLKVNHEILYHLQLSPAERASIQQR
                     WADVLHNKKRNVVVIDYPKYMQAIYDIINSPATLNNLNTRSGMAPLAFALAALSGRRM
                     IEIMYQGTFTVSGKYTVDFLGQAKKRTSDDITRKIYTLCEAKTFVYLINVLRSCPAAS
                     DFDDVVKGYGDNDTRSENGRINAILAKAFNPWVKSFFKDDRRVYKDSRAIYARIAYEM
                     FFRVDPRWKNVDEDVFFMEILGHDDENTQLHYKQFKLANFSKTWRPNTGNENTRLEAL
                     QQLDDEMPGFARGDAGVRLHNTVKQLVEHDPSIKITNSTLRAFKFSPTMISRYLEFAA
                     DALGQFVGENGQWQLKIDSPEIVMPDEEELEAIPEQTDELSEDDDLDDDEIEIEEEEE
                     EESVEEQQPPAPSSPVSKPVFKPVRNIGDGSYVVEFHYNGQNYAWSGSANSPISAMQL
                     AWLTYFG"
     gene            19345..20511
                     /gene="sopA"
                     /locus_tag="ACHUZQ_000670"
     CDS             19345..20511
                     /gene="sopA"
                     /locus_tag="ACHUZQ_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFR1024455.1"
                     /translation="MSLKKLLQDCVTRGQEMTQAIAIAQFGDDSPEARRITRRWGITE
                     VADLIGVTPQAIRDAEKSGRLPAPDFEMRGRIERRAGYTIDQINSMRAVFGNPNQRPQ
                     DKNPVALAVMSHKGGVYKTSSAVHQAQWLALQGHRVLLVEGNDPQGTASMYHGYVPDL
                     HIHAEDTLLPFYLGERDNAEYAIKPTCWPGLDIIPSCLALHRIETDLMQYHAQGKLPH
                     PPHLMLRAAIESVWDNYDVIVIDSAPNLGTGTINVVCAADVIVVATPAELFDYASVLQ
                     FFTMLLDLLESVDLGGFEPTVRLLLTKYSLTTGNQSRWMEEQIRNTWGSMVLRQVVRV
                     TDEVGKGQIKMRTVFEQAANQRSTLNAWRNAVSIWDPVCREIFDDLIKPRWENE"
     gene            20511..21476
                     /locus_tag="ACHUZQ_000671"
     CDS             20511..21476
                     /locus_tag="ACHUZQ_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFR1024456.1"
                     /translation="MKQRSLLKNAPDINRIMSNNHHAPEHQPVSPMVGDLKRQLSSLT
                     GNSITLPVCGRNVNFKLETIPADKVEMATMVWLGNERDQDLLNESSLADLIPSFLTSG
                     QQNPAFARKVSGIVEVADGSRRRKAAILTGCDYRVLIGDLDDEQMHWLSQIGNDYRPI
                     SAYERGKRYLRKLNDFDGNVKALAEAENIDRNIITRCINTAGLPKDILAIFNHPGELS
                     ARAGDSLFKVYKKNMAAMSNAAHHLLAIKKNGEDLEASRIIQILSDSVLVGDEEKTKD
                     EKKYGEGITARYKGNFVTIKIDSRKISKEILSRIELLLEEQSTDN"
     gene            complement(21509..22084)
                     /locus_tag="ACHUZQ_000672"
     CDS             complement(21509..22084)
                     /locus_tag="ACHUZQ_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1024457.1"
                     /translation="MAFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     NSLLEAWKKYRVLLNRVDTSLSPDIEWPSQP"
     gene            complement(22084..25482)
                     /locus_tag="ACHUZQ_000673"
     CDS             complement(22084..25482)
                     /locus_tag="ACHUZQ_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1024458.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHAADAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAAQSASAAADSKTAAASSASAASTNAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVDMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(25547..26146)
                     /locus_tag="ACHUZQ_000674"
     CDS             complement(25547..26146)
                     /locus_tag="ACHUZQ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1024459.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKRHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(26213..29611)
                     /locus_tag="ACHUZQ_000675"
     CDS             complement(26213..29611)
                     /locus_tag="ACHUZQ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR1024460.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASSNIKPGYDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLNFYKGLINKTHLGKELLENFELTEDNASRLEEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(29672..30244)
                     /locus_tag="ACHUZQ_000676"
     CDS             complement(29672..30244)
                     /locus_tag="ACHUZQ_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1024461.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(30241..30984)
                     /locus_tag="ACHUZQ_000677"
     CDS             complement(30241..30984)
                     /locus_tag="ACHUZQ_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1024462.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            complement(30990..31688)
                     /locus_tag="ACHUZQ_000678"
     CDS             complement(30990..31688)
                     /locus_tag="ACHUZQ_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1024463.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(31688..32017)
                     /locus_tag="ACHUZQ_000679"
     CDS             complement(31688..32017)
                     /locus_tag="ACHUZQ_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1024464.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(32014..34575)
                     /locus_tag="ACHUZQ_000680"
     CDS             complement(32014..34575)
                     /locus_tag="ACHUZQ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1024465.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            complement(34568..35002)
                     /locus_tag="ACHUZQ_000681"
     CDS             complement(34568..35002)
                     /locus_tag="ACHUZQ_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1024466.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            complement(34984..35406)
                     /locus_tag="ACHUZQ_000682"
     CDS             complement(34984..35406)
                     /locus_tag="ACHUZQ_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1024467.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGSAEPVSAGKCSTVS"
     gene            complement(35422..36162)
                     /locus_tag="ACHUZQ_000683"
     CDS             complement(35422..36162)
                     /locus_tag="ACHUZQ_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1024468.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            complement(36170..36565)
                     /locus_tag="ACHUZQ_000684"
     CDS             complement(36170..36565)
                     /locus_tag="ACHUZQ_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1024469.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(36562..37140)
                     /locus_tag="ACHUZQ_000685"
     CDS             complement(36562..37140)
                     /locus_tag="ACHUZQ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1024470.1"
                     /translation="MVIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLPRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(37152..37505)
                     /locus_tag="ACHUZQ_000686"
     CDS             complement(37152..37505)
                     /locus_tag="ACHUZQ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1024471.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(37517..37912)
                     /locus_tag="ACHUZQ_000687"
     CDS             complement(37517..37912)
                     /locus_tag="ACHUZQ_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1024472.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDGT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(37954..38979)
                     /locus_tag="ACHUZQ_000688"
     CDS             complement(37954..38979)
                     /locus_tag="ACHUZQ_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1024473.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(39035..39367)
                     /locus_tag="ACHUZQ_000689"
     CDS             complement(39035..39367)
                     /locus_tag="ACHUZQ_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1024474.1"
                     /translation="MTSKEIFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(39377..40696)
                     /locus_tag="ACHUZQ_000690"
     CDS             complement(39377..40696)
                     /locus_tag="ACHUZQ_000690"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1024475.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPATLALSVNDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(40677..42278)
                     /locus_tag="ACHUZQ_000691"
     CDS             complement(40677..42278)
                     /locus_tag="ACHUZQ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1024476.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(42275..42481)
                     /locus_tag="ACHUZQ_000692"
     CDS             complement(42275..42481)
                     /locus_tag="ACHUZQ_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR1024477.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(42478..44403)
                     /locus_tag="ACHUZQ_000693"
     CDS             complement(42478..44403)
                     /locus_tag="ACHUZQ_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1024478.1"
                     /translation="MNISNSQVDRLRHFVRAGLRALFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPASVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDSAAT
                     NKKTLADYARALSGEDE"
     gene            complement(44378..44923)
                     /locus_tag="ACHUZQ_000694"
     CDS             complement(44378..44923)
                     /locus_tag="ACHUZQ_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR1024479.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRLASEADLHPGTLEFERHRLTRAQATAQELKNA
                     KESAEVVETAFCTFVLSRIAREISSILDGIPLSVQRRFPELDNRHIDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            complement(45063..45287)
                     /locus_tag="ACHUZQ_000695"
     CDS             complement(45063..45287)
                     /locus_tag="ACHUZQ_000695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024480.1"
                     /translation="MIRGFVPHARQLCAGVEETGHVFRSVRPWSHDAGLSDIEFIANK
                     PERLRVLSGDPAGYGAEGARVFAIYENFPV"
     gene            complement(45521..45970)
                     /locus_tag="ACHUZQ_000696"
     CDS             complement(45521..45970)
                     /locus_tag="ACHUZQ_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024481.1"
                     /translation="MQEEAKKILVDLLNNASDGIDSAIAFSQAQIPDVVHQLLVWNIV
                     DSLIKTLIAISTIPLVIWFMKKQCKKVEIGKFDNDGRSCDNGQPKYKPTMIWESDGRL
                     SAFVLPLVAVFILWLSFIISIVANMTWLKIWLAPKLYLIEYAASLIK"
BASE COUNT        11206 a        12292 c        10682 g        11847 t
ORIGIN      
        1 aaaaaaaaat atctcaggct ctctttctat aagcttttag ttgaggatgt tctgcaaaac
       61 aaaactggtt agttatgtaa ctgcttctgg aatacaacag ccgacgtgct acaacgtaaa
      121 attcgtttga ttgtggcagt tgaggaaaca gacttgcatt tctcaatatc atcaatgatg
      181 accacatccg gtacaggcat gatgcgggta tgcaatgttt ttgcactaat aacatcacag
      241 ccattcagtg gacgaatatc tgcatcttca ggcactacca gaagcacacg tttctccgtt
      301 gcagaaaagc cagcagcaag agttcgcgac aataaagtca ttccactacg tggcttcata
      361 caaatttcag aaatatgatt tttttcgtcc aaatgatgct ggccatcatt tttatcatca
      421 ctcaacaact ctgctgttaa tacaatccct gctgtctcag atttccagga aatgctgata
      481 tcctgatcat ctgcatcaac atcatatttc atcgagtaag gactaaagca acgtccaacg
      541 aacgcatcgt ttaccagtac acgcccaccc tgtctgacct gaaagtcaac gcgccctgaa
      601 agctgattta ctgttacaag caatgccttt tttctttttg ttttattact cattgccaca
      661 aactgttcag ccatctctct tgtcacacta ccagaaactc tttcttgaat tttcacagta
      721 aattttctgg atgtagtagc atcgacatta aaaaatctgg atgtcattag gcagttatta
      781 acaattggca tcagctcact tacgattgta acaaattccc ggtaaagctc attttgcttt
      841 tcagcacaca gttttccgca atcggccagc ttaagctcaa gagcaattaa tcgttccatt
      901 attgaattaa ttttttgcaa ctctttcatc aaaacctcct gacaaagata ccgtgcgacc
      961 acggtcgcac gctcctgaat acatgccctg tttcttccac cctcgcacag gactagcgag
     1021 catgagggac aaacccgcga atcattagcg cggtaaaaac ccggtgtgca tcgtttttga
     1081 ttatttccgc acactcgcgc agaggagttc cccgtcgggc tacggtcata gttaatgcgg
     1141 gaatacggcg acgatgcagc gcggaattta gcgagcgacc acgatcgcac acgagaaaca
     1201 aagagatttt tatactgcag aggaacaagc tggcgggtaa ttcagcgtag tgaataccgg
     1261 catctccatc ttgtatgcgt aatgatactc agcagttgct ccagcagacg ctttccagcc
     1321 aggaagcatc aaaatagcat ccgcacaacg aagcattgca aagcaaatat ccatgtattc
     1381 acgttgtgtc agaccattag gcaaagtggc tggattcaag acagagtgac catgtcgtga
     1441 caaacgatct gcctctttat taaaggcgtc acgattaaag ttctcatatc ccgtcattgg
     1501 cccggcaata taaattttca ttcactatcc aacaaattga gttcgttttt gatacagagg
     1561 gattaattca ggcattgcgt attgatgtat tcctgaagcg ttctcagtgc tgtttggtcg
     1621 cggataattc cgtcccggat accgagaacg tttcgtccag caactgaaga gagttcgacg
     1681 gtggcatcat tgcccatgcc ggaggcgctg gaggttttgg ctgaggctgg cacagggcat
     1741 tttcctttga cgagcacccg accaccatta tcaagcttac gccgaagagc atcattttca
     1801 gctttcgcat tggctaactc cttcgtgtat ttagcatcga gtgcatcagc agcacgctgg
     1861 cgctgctgca tgtcagtaat ggtggcggtc gcctgcttca gctctctggc gtttttgtcg
     1921 cgctgctctt tgtaggcgat ggcgttatca cggtaatgat taacagccca cgacaagcag
     1981 acgatgatgc cgataaccag agcggagata atcgcggtta ccctgctcat tgttgacccc
     2041 acaaacagac ttcacgctca atctcacgac gagtcatcag gcctttccat tgcttaccgc
     2101 cagcatatat ccagcgacgt agctgatcac atgcgccttt gatatcgccc tggtttattt
     2161 tgcgaagaag cgtcgatgtt ctgaaattac cagcgcccac gttgtaaacg aacgagtaaa
     2221 gagcgccgcg cgttgtttcc ggtatatcga ctttgatgta cggattaatt tgtctggcaa
     2281 cagtggcaag gtctttattc aggagagctt tgcattctgc ttcggtatac gttttaccga
     2341 gcatgatgtc ttttccagtg tgcccgtaac atacagtcca tacgccaaca atatctttgt
     2401 atggtatgta actgacacct tccagaccat cgttaccact tgggccagtg atgagcatag
     2461 acgctatggc aatagccccg ccaccaatag cagctgtgac agctttgcgt aatgatgact
     2521 gcatcatatt tccttcggtg ctctcttccc cagctctcca ataaccatcg ctgtagcaga
     2581 aagattattg gtattagttt tatccaatat gtcctgcaat atcttcgtgc gcttcatctg
     2641 ctcgcgttta ttgagccggt atgtaagcac accgaggata atgctgaacg cgacaccgat
     2701 aatgaagccc caatcctgca atgacaggct ggcaaagaac gcagcaagac cagcgctacc
     2761 ataagatgca ctgctgtatc gttcatccat cttcatatct cttacctcgc aatagttgct
     2821 gaggtttcgg cttggaaact atatggtgaa aataacccct accaaaaacg gcaggggaat
     2881 atattctcca gttactcaaa taactttttc acttcatcaa ctgtctgaag ataacgctcg
     2941 ccctcaatct caatacctat tgcctgacgt cctaacttca gcgcagcttt aatagttgag
     3001 cctgatccca taaagaaatc ggcaacgata tctccggggc gtgaactact ctttataata
     3061 tgttccatca tcgctgcagg tttttcacat gggtgcttgc caggataata ttgcacagga
     3121 ggaaactccc atacatcggt ataaggtaca tcagcagtaa catgaaatgg acggcgtaaa
     3181 ctttcatact gagccttcaa atcatcatac tgcttcttta cttcctcata atgcaatttc
     3241 agatcgctat aacgcttttc atgttctgaa taatcaacat taaaaggata tggacacggg
     3301 acaccgagtg aatctgcctt cctgcgaaat aattcgcata gtttattaaa atcaattaat
     3361 gatggaagcc gccactgcga ataagaaaac cagtgtgaac acatctgctt tcctgtcgct
     3421 gaattaatgt ctgcggctga tatatctaac tgacggcgag ccagcgaaaa agcatcaatt
     3481 agtggagaaa aaatattttt tcttaaatct gcacattttg aagcataacc tgactgacct
     3541 ttagcataac ctgacgcgcc gtaatgctct gcaaataata ttctttcagt agcaggaaaa
     3601 taagccctta aactttcttt atgacaaccg cgccacatac cactgggttt agcccaaatg
     3661 atatggttca gaacattgaa acggtcgcga gttaataatt caatttttga cgcacggcga
     3721 gagccggtaa acatatacag gctgccatta ggggccaata tccgccaaaa ttctgccaaa
     3781 aattcgtcaa tccaccccaa aaagtcagca tcgctatccc actggttatc ccatgcgtta
     3841 gcctttacgc cgaagtatgg tgggtctgtt gcaatcaaat tgaccgagtt atcagggatg
     3901 gttttgataa attgcagaga atctgcgcaa acgagttgtg cgccatgaat attagtggtt
     3961 ttgaacatag ctattatttt tttgcctggg taagctaacc cagcgatgcg catcgcgggt
     4021 gggctttagg ttcagcctat agctctggca tgggttgacc gcaggacaag ctgcaacttg
     4081 tcctgcgccc acttttcagg cacaaaaaaa ccgccataaa ggcggcttgt tggctattgg
     4141 gtacaaaaaa cccaacttaa caaaacaata actaaaaacc gtcattttgc caagcttttt
     4201 cgtttttgtg ctgtgcgacc gtggtcgcac agtttttaga aagttgcctg cttatattca
     4261 tcggtcaaag aatatcgacc agacccgcta cgctttgcaa ccccaaaaca gatcatctgt
     4321 tcgatgataa attcaacggc agcctgactc aaaaaacagg cttcgcttaa ctctttcaag
     4381 gaaatgcgtg gatatccacg cataacacat tccacactca gggccgcttc ggtcatattt
     4441 ccacgaattt cattaatatt cataacgcct ctctaatcaa tcttgaaact ggaaatcaac
     4501 atcagccata aagcggttca actcagccag ttttggcccc atagtcccaa ttaagcggcc
     4561 agccagtcgg tctgtcaggt tggtactgtt gaaaccatat tccttttcga aacgttcaac
     4621 cttctgccac aactcataca attcgttagc tatctccgca acgtcgccgc gcattttttc
     4681 gttaccttga taattcataa tatcctcgaa attcatatag ttagtgggtt atccccctca
     4741 acaacacgaa ctgtaactct gccaaaagaa cacatccagt gttattttca ctttttagtg
     4801 aaattttata tttgctttgt tttctcgttt tggtgtattt tgcgcgttag gaggctataa
     4861 tatgtttaac gtgataaccc accctgcagc actggatgaa ctgcttgaac taccagacga
     4921 tctgcgaggt cgcatgacca ggctaattga acgactggag aaagagggaa acaaacttaa
     4981 gatgccccat agccgtgtaa taggcggagg actctttgaa ctaagagtag gggataaaaa
     5041 catcgcaaga acgctatacg cctacgcgac tggtaacgaa atttatctat tgcatgcatt
     5101 tgtaaaaaag acacagaaaa caccagtaaa ggctatagaa atcgccagaa ctcgcttgaa
     5161 ggagatgaac tgatgaaagc aaaaggcatc ccgtttaacg aagtaaagga aaaactactc
     5221 aacactccag aagctatcaa aggatatgaa gaggccgata aagaactgga gatggtcgaa
     5281 atgttatacg aaatgaggga aagagcaggt ttaacgaagt cagctctggc agaacgaatg
     5341 ggattgcgcc cgtccgccat cagccgcctg gaaagcaatc ctttaggggc aagcatgaaa
     5401 actttatcaa aatatgccca agcatgtggc gcaacaatta atattaatgc cgtctattga
     5461 ttaatcactt ttcaccaccc cgtccagaag ggcggggtgt gtatcaatga tgtaaacact
     5521 ccacagcaat cgcttgttgg caattctgag caatctcccg cgacatacta acaagccgtg
     5581 aaatctcgcc aagatgcaag ctagcttcgg gagaagccag agcaacgcta accagatcca
     5641 aaacggcgtc gatgtctttt aggtttgatc cgagttcttc aagagttgat acagatgctt
     5701 cagtcattga atctaatacc atttaattaa aaaaggttat ttgatactgt ataaaattac
     5761 agggtaaaac tcaaatttta tagccaactt tcaatggatt aacagggtta atagctttgt
     5821 agtccgatac ttatgttctc ttcctacgcc tcactcaacc acctacagaa gcccatcatc
     5881 accataagaa aaactctttt cgcttcgttt tctcatcatt tcaaaaacta tgcctttttg
     5941 tttatttatc attccgtgac cagtcacact ttgagcacaa gtttcagcct cgtagttgta
     6001 aggtgacatg agccttcacc atcaaaaata cattctgaaa caggcatttt ctgaccacat
     6061 cgcgggcaag tagtagatag ctgcttctgc agctccttat agtttttcct gatcagcaga
     6121 ccgatgactt cattctctga atatggaact cgtcccggtc tacgcaatac acaaatttcc
     6181 cccaacatac gtagctcgtc aggttccaga atccagtccc gtcttgatgt gccttccttc
     6241 ttcaaccgtt ccctgcgtct cctttgccgt tctgcagctg tcagcgccat aaatcctcct
     6301 gaaaattact taacccaatt aactacaccg ccagcggcca caaccgcttt ttcacagtca
     6361 cgctcatatc tcaaaggttg aaatacacca tcaataaaat atttatctcc accacatgct
     6421 gcaggaagtg aaacagaaaa ctgaatatga ccatccggag ttaccggaga gttgccagcc
     6481 tcatacgcga cccttatcca gtgcataagc atttctgtac ttacacatcc acaatcaaca
     6541 tcaattttgt catgctgctg ttccagccat tgctcaaaag ttaacttgta agtttggctt
     6601 acaggtttgg cttccagttc tgctatgtga tttttggctg attccaactc aatcgacaat
     6661 ttttccaact gctctttatg cttcttgtat tcctgatatg cgtgccaaga ctgacctttg
     6721 cgcacactat ccgtgatatc agtaatctgt tctggtgtta gcgtggtcag tggctgtgct
     6781 gggaaaatca gcactttccc ggaatcccaa tcaaaaccag cgtgaattga ctgaacctca
     6841 actgaaggtg ttgaaccaat gctgccaggc gaatgaacaa cgatcgttac atccatatcg
     6901 cgacgatcgc tgtggttgtt ggacaaaata cgattcaaca aaatacgatt caccaactca
     6961 gaaaatttag aaaatttcat gctgattcac ctttctctgc tctctcctgt cggaacatca
     7021 ctatcatcag gtcgcctttt gtcgctatct tggctgttgt gcctggctca atgcggctaa
     7081 gttcaaatgc gtcatagaac gcctccaacg ctttctggcg tttttcctgt ttgcggcgct
     7141 ttccccactg ctttagaaaa acagaggccg cccacttccc cgcactgaac atgatgtaaa
     7201 accaaccgag aagtgccaga ccggtattta gggccgtatc gatcgttatc gtggtgtcga
     7261 tattcactac gccacctcct gaaaatttcc ctgataaaac gccaacacac gctgcataac
     7321 ttcgctcttc cgacaatcgt gacaaatcat gttcaagcgc ctgtcataac gacgtatttc
     7381 tccatcaggt aatgaccaga taaggtcagg atcaacaact accgatttct tcggctttac
     7441 tcttgatagt tttttacggg cgttttgcca gtccttgcgt gcttgtgcag acagaaatat
     7501 cccggagcca gagatataca aatcaccact ggcagcaagc tctcttgata aacgactaac
     7561 agcacaccgg ctaataccag tttcatccgc aagttgccta accgttcctc gtccattcag
     7621 gcgcacgagt tccacaattt gtttattcag ttcttccctc tgttctggtg taaaagcctt
     7681 tgccattacg attctccttg cccttttaca agacgggaga aaattgcgga tacatattta
     7741 gcctgatgaa tagcatcagc cagggcattg tgtcgttcgc catcaaatgg catatctttt
     7801 ttaggattaa aaccaataaa ctgaccaaga ttaacgattg ttcgaacatc ctgatcatta
     7861 aaatattccc acggacaggc taagccaacg cgctcataag caccgcgtaa aattacatta
     7921 tcgaacgttg ctccatttcc ccatactttc aactttttta aattacatgc atggcaagta
     7981 ataaagtgtg ataattcaaa aagtgcatcc ttaatatcaa gggcatcatt agtacaaata
     8041 gcggctctgg cttccttgct ctgtttcaac caccataaaa tggtatcacc atcggcgata
     8101 ccaccattca acatcgagct tctgagattc actggcacat aaaaagtagg acctaattcc
     8161 tcactttctg gagaaaaaaa cactgccccg atagaaacaa ttggagcaca tgtgttagta
     8221 cccattgttt caaggtcaat cattacatca gaccgtttcc tgtcgtaaat aatctgatta
     8281 gtcacgaata attctcctca acgtttatct atacccaaat tttctgttga tatgttctgg
     8341 atggttttgg cgttctttct gattcaggta aatgaacata aacgcaatat ccatcaccta
     8401 aaaaatcaga acgaacaacc agaactttct taccacgccg ccgataagta tctgaaagac
     8461 gctctgcttc atcatgcgtc atcggcccct gcttaaatgg cgttattttc attttcttgt
     8521 gcgaccacgg tcgcaccccc tctgatttcc aagtaacgct taatccaaag attttctata
     8581 tgcttattac ctggctgatt tgataaatac caatcggcaa ttacagactg cttattacta
     8641 tctggtcgtg tcctgtaacc acaagttgga caccaaataa catattcatt tcttaccccc
     8701 gaatacctta attctggctt cgacggtttc ctgaacataa cttgtttaca aaggcagaat
     8761 ggcacttcct gcccgatggc gtttgatgat ttcactgcgc ttctccgccg catttaataa
     8821 tgcggttcga tttaagctca gacatcgcga cttaattaaa agccagcgtt ctttataatc
     8881 ttgtctccag ctatcaatag atatattcag tagaaaactc agatgcctgt cctgttctct
     8941 ctcatcaact gcctcagcac acgtaagttc tgatttaacc tgttgtgccg cgtaataaat
     9001 caatttccgc atggcttttt tcgtggaggc cttcattttt ttaaagccgc cccccgaatg
     9061 agcaaccaga aaatcaagcc ataaccactg acaaacaatc acatcattgt tgtaatcagg
     9121 cttgcatccg tagcaataat aaagccaggc tgattcctcg ttattcaact gaccaatagc
     9181 cctgcgccag ctggcgctct gaaaatcaat atcagtgagc aacattgatg attgtttaaa
     9241 tgttttcccc tgatggaatc taaccggctc agcaggaacg gacacttcgt atgagttatc
     9301 atcccccaca ctgattgacc ggactggttt attgttaaac cgtcctgatc taccgagacg
     9361 catctgctca agctggacct ccagaatgcc acgctggcga taatgaacat cagccaatgc
     9421 cgtagagacg cagctgcgta tatactcaag ctccacgctt acgactctcc ttaatcgact
     9481 gctttacccg ccgagttgta aagtcactct tcagcttata agcagtacga acttcaatat
     9541 cgctttgccg caatgaagga atctctccag cggttaacca ttggtaaaca gctccgggtg
     9601 ttacacctac ccctgccgct gctttttcta cactgccaaa atggcgaata agttcttctg
     9661 gtttcatgtt gttattataa acaataaaca acaattaaag ccagctataa taaataaatt
     9721 ttatagccag ctataagaag atcatttatg attacagtat gaaaacacga ggcgaacgac
     9781 tgaaagcacg ccgtttagag atgaagctga cgttgcgaga agttgctgag gctgtcgata
     9841 tctctatagc aggcgtccaa aacttagaac gtggcgacgt aatgccgtca ctggagatag
     9901 gtattgccct ggctaagtgc ttacgcaagc cagtgcattg ggtgctatat gggacagaat
     9961 atgaccctga tcgcattcct gttattggca caactgaaag tgggccggat gaagagtgga
    10021 agccgggaca ggttccaaat actgagcgat tcctgccatt catcagccag cgcaatactg
    10081 tgtatgcgtt aactatcagc aatcttacgc agggaaacta tcagccaggc gattttctcc
    10141 tggtcgattc tgcgctggaa ctggtccccg gcgaggatgt tctggttcgt gatactgcag
    10201 gaaatatcac tatccagcgc ctggcccgtt ttgatgatga aaattactat ctggatggaa
    10261 caaactcaca acgcgccatc ttcaggaaaa gcgatcttga atttgttcac caaatagtcg
    10321 gaacgatcaa atcattcatg gttgagggta gatgacttaa tacggggttt attgtttgct
    10381 gtaaatctgg tttaatgcag ggcatatgtt ctgtgggttg actcaggtta cagcagcaga
    10441 aaaaagacga aaaaaaaccc gagtcggcaa actcgggtcc ttttcaggaa gcagccacag
    10501 taacgcgacg gattccgtca attcaagatg cgtttattgt ggctgctcct gcggattttt
    10561 tcaacccgaa aaaacataat tcgcatggaa tggctaaaaa atgacattaa acgatttcta
    10621 tgcggatcgc tttggcagcg atccgttctc gctaattgaa gcagcacggg atgaactaac
    10681 cgagctggca cagatggctg gtatcaactg gcctgcctgt tccgacaaca tccaactcaa
    10741 ccctcgcggg ggggttgagc gttactcttc atacaataaa acatctccag aagctctcga
    10801 aaagagcctg aaaggacgcg tagaaattta ctccaggctg gaacacagca aagggattga
    10861 ttacccgttc attaacttcg tccataaagg cagcgatgct ggctcatgga gcggtttctc
    10921 cttcctgttt tctgaatatc gccgtgagca acaacggaat aatgcgaccg tggtcgcaca
    10981 accagaagaa gaacgggaaa gaatagaacg tcaggccgaa gcacgtcgtc gccgtgaaga
    11041 acaaaaacga attaatgacc tgaaaaacaa ccaaatggac catgaacgtt tgctgggatg
    11101 gctggcattc cacagcgcgt gggaacatgc tcaggctgaa gacggctcat ggccatatgc
    11161 gattaaaaaa ggtattcgtg acgtttttag ttcatgtgat gtgcgtcgcg tgaccagtca
    11221 cgacagtgcg aaatggagcc gggggccaac cacatatatg gctatccctc tggctcacct
    11281 ggacggtcgt tatgatggtc gtattgtggg ttggcagcga atcgacaaac aaggtgggaa
    11341 ataccagacc agcgcagtaa ccagcggtga tttcgtcggt gcctgctttg ttatcggtga
    11401 gctgaaaggg gcgcaaaaca tcgctgttac agaaggtttt gctactggtg catccatctg
    11461 gcttgccacg aaaaaagata aacgctttga cgctgtggtt gtcgctgtat ccgctaacaa
    11521 catggttcat gttgtggaac aactggtaaa catgtacccg gcggcaaaaa tcacctgcgc
    11581 actggataat gaccggaaat ctgccacaga aggaaaaggg aataccggcc tgcggactgg
    11641 ctatgaaatc ttatcgaaat tcaacgatgt ccgctgcgtc tatccaactt ttgaagacga
    11701 tccgcaaatg gagtgcagtg acttcaacga cctgcacaaa ctacgtggac ttcgtgaaac
    11761 agcacgtcaa ctatttgcaa cagcaaaccg cctgaaaacc ggtactgatt tgctatctct
    11821 gacgctcaac cgcctgaaga caattaagcg ggataaccgt cgtacttttg ccaaagagct
    11881 gctgagagcg gtggatattg gcatgctaac ctgtccggtt cctaacagtc cgtcagattt
    11941 atttaacatg ttctgcatcg ttctgcgtga aatgggactg gaaaatatct atcgcgctac
    12001 ggtaaaagac cacattgccc gtcgcctgaa taaaaagtgc cgtattgccc aggctccgcg
    12061 ctctttcagc gaacgcataa ctgatcccaa taaacgccct caacatatca cctataagcg
    12121 ttttgaaact tcggtaatga ccgatgatgt cctgaagtac gtgcaatcac tacaggggat
    12181 agtcatcgtc cgtgctggta tgggttccgg caaatctact ggattgttac gaccgttaat
    12241 gcatagctca gaacgtggta tctctgttgc tcaccgtgtc tcccttattg gtggcctgtg
    12301 ggaaatgatg acagaaggaa aaggtgtaaa agccgatatc ctgcactatc aggaccctgg
    12361 ctatcaggaa atggctccat acgccagtaa actgaccatc tgcataaact cgattgttaa
    12421 aaactgctgg caaccgttga tgcgccagca cgactatttt ggctttgacg aggccacaca
    12481 agggctacgt gccgttcttt ccggtcgggc tatggaaaac ccggttgctg tattcaatac
    12541 gctaattgac gcgctggcca gaacggaaca acacccaatc atggtggatg ctgatgcgaa
    12601 cgatctactg gttgacctgg ctgaactggc tatgaaacgc cgtgaagaac taggtttacc
    12661 ggcatggctg cagatccacg taattgaatt acctgttgat gttcgcaacc gcgaaacagg
    12721 tgagccaatc cgggtttttt atacagaaaa agatcgcatc atgaccgagg tggtgaaagc
    12781 tgtatcggat ggcgaaaaaa tcatgttggc caccgacagc tccacctttg cagaagatgt
    12841 gacagccacc ctgagaatgc attttcctgg taagaaattt ctgtgtgtaa accagaaaaa
    12901 caaaccagag ccagaagttg aagcatttac caatcaaccg aaaaagatgg tcaaaaaata
    12961 cgacggcctg atttatagcc cgtctatttc atccggcgtg tcgattgagc aaaaacattt
    13021 tgaccgtcat ttcggcatgt tctgtggtga agttgtaccc agcgacgcag tgcaaatgct
    13081 acgccgcgac cgtacagcaa aagaatttat tatcggcttt gataaagctc gtgcaaaacg
    13141 tgaaactgac ccgcaaaaaa tagaacgagc attcgcccag gcattgcttg ctaccgcagg
    13201 tatgaacggt gaacttacgg atgtcgtttt cgatggcgac cgtatatcaa tgggggtggc
    13261 caataccgac tttaccagga tgaaaattaa agcagccgca attgaagcga ctgcgcgtaa
    13321 cgactacgcc agcaacatga tctgcatcat gcacagcgac ggttataagg tatcaccgct
    13381 ggccaccgat tatgctgcga atgatatcgg taaagatctg cgtaaagaag cgcgggatat
    13441 tgtgtgggaa cagacactgg atctgcacct caatacagaa actccacaag aatcagagcg
    13501 tgaagaactg ctaaaaaaac gcgcgctgac gcttgaagaa caggcaaaac ttgtccgctg
    13561 ggatattgaa aacgaactga aactaccggt tgaagaaggc acgctgaaat tctatttcga
    13621 tggtgctcga gacaaagttc gccgctatga aacaatgctt ttagatgagg taacagctcg
    13681 tcgttacgac cgggaagaag cagccatcaa ttttacctac gcataccgtc aggcgggaca
    13741 gtggcaatat tttgtagtga ccgcgatgac tcgcgagcag gctgatgatg tctttaaaac
    13801 taaattccct ggaataatcg atcacaaggt aaaagctact cctactgttg aagtcggggc
    13861 gcgcggtttc tatgcgctta aatcagcaac tctccggcag tactttatcg actgcggtat
    13921 caaccctgag actatgaccg gcgaggcgac tcaggagcgt ctcaaattcg ctcgagataa
    13981 cctcatgaca gccgaacgcc gtgacctgtt gaataacgtt ctgcacattg gtggttttat
    14041 gacgccaaaa gggaagccta aagtgccgga agcgttgttt aaaaacattt gcgactctct
    14101 tggccttaaa acaggtaaac gtcgcggcag agacggcgat aagcgtccaa ccttgcgttt
    14161 tgttgagcct gaatcagcgg catttatgct ggatattatt gttcagcgtc agaaagatgg
    14221 cctaacccta aaaacgcgta aagccgagaa gacagccatc gaaatggatc gcgatttgga
    14281 tctcaatata tatatggatg ataaaacgcg atccacaaac gagcaggttc aaaacgcccc
    14341 tcattcagta atttctgagg ctttggccgt gttgccagtg gctgtacctg agtcctgggc
    14401 gatgagtgcg ctttccagtg aggagttggc cactatgtca tcatggtcat cagccagcat
    14461 agctatgacg tttgcatccc tgtacctcac agagttcatg gatcaccttt ccagcaatga
    14521 gctgcggcgg ttacgtgaat acattacggg gaatagctca tgcagctacg aagagcaggg
    14581 cgctttctat gggtaatgag tggatattat ctttgtgtct cttgctttgt gtgttccagc
    14641 aatggttttg gttcatagac cgaaagtctc acaaggaaaa catgaaggcc aggaaggcga
    14701 tgtttcgtgg tgaacaattt gcgcagactt atcgtatgtg gaaacgtttc tatcgtgaat
    14761 tttcggtgaa cggggagtaa aaatatgtct actactgcat ttttggacaa acttgattac
    14821 cgtcagctgc agttctgccg ggatgaatgc gaacgacgca ttagggctat ccaggaggaa
    14881 gaaaaaaagg tcgcctgggc tgtaacagat cgtggcctta tctttggctg gttccgtacg
    14941 gaggattatc tgaaagcagt ggaattattg gcgcgtaagg ctgctgagcg ttgggaacga
    15001 gccgacaagg aaaacccgga gacgaagtat gaactgaatc tctacatcga gggcaaccgt
    15061 ctaccattgt cagagtataa cgctttgttt gctgatggtg agtggggtta aaaatggctg
    15121 ttcaatcata caaatacgaa tcccctttgg ataagggata cattcgtatc aagttaagtc
    15181 gttatcagca taaggctatt tttaaggtaa ggaaaattcg catattggat gcgtatgcgt
    15241 attactacaa cggtgaaaat gttgttgttg aacactttct tgcacgatgg tttgtagctc
    15301 tgatttttgt ccctctcctg ttaattggta cttttgtgga tggttttcct agtacatggc
    15361 gggagataaa gaaaggccta ttcccgcgta agtacggcag attttcgcaa gatagctggc
    15421 gagttatacc tggtaaacat tctgtagatg agcaaccgat tgttgatttc tggttgaatc
    15481 gtatggctga tgctaaaaaa gttgattgaa ttgtgatgac tgttacagga gataaagtga
    15541 agagttttat ttttgtttta tgttttgcct tttctgctgc ggtttatgcc caggaagata
    15601 gcatttctgt tattaactca tcagcggacc ttgcatgtaa aaataatccc gatgctgcat
    15661 caaaagagtc atgcaaagag ctgctccttt cgacggctgc ttttgcttct gaaaatgcaa
    15721 aattttattc agccttttgt cgcggctatg ttaaggacga agataaagaa aaatgtgatg
    15781 atgccggtaa gttagttagg tatttccaat ctcttaatca ataatggcct ttgattaacg
    15841 atcaacttct gatcactcgt tcaagcaaac tataaaaaca cctgtccccg tgactggtca
    15901 cggggtaaaa tgtctttgca aattattgac gtgcgcgtcg ttttgacggt aaagtttact
    15961 ccgctacggt aaatcccgta gccgggcgta ggaacccgag taaatgaagc gcacaacacg
    16021 cgccagcgtg tttttttgtg tgtaaaatct gcgtacacca aatttatggt ggctcagatg
    16081 gggcagcttt tagctggccg gtttcttcgt ttaccggtat tcctaccccc gtctgggcta
    16141 ccgccatata gagcgtagga actctggtgg tagcacgacc aattaaacgg agtgcatacc
    16201 atgttcaaat tcaaatttgc ggcaatcgtt cgcacagaca aaaagtcaca catccatcat
    16261 ctttccacca tcgcgtcatc tgaacgggaa gctcgtcgcc agttcgccag ccggtttgtc
    16321 ctggtattgt ctgcccgtat tccggtgcgc gaggtggctg catgaatcag gtaaaaatga
    16381 acactgttgg cctgctggaa tcgttggatg aacgtctggc tcaggtctat gcgctcgtat
    16441 catcagccca tcggacaatc tccagttgtg aggcgtcgat ttatctctca gaagcaggga
    16501 aactccttca aattgcgtgt gagctaacac acgaagcaca gggttattca gcatcgctct
    16561 cgtcgcaatt gctgcacgat aaggaggctg aatgagcgcg ttagcagtct tttcctttca
    16621 ggaagagcat caggttcgtg tagtgatgat taacggcgaa ccgtggtttg ttgcatctga
    16681 tgtgtgtatg gcggcgggta ttgattctac ggccatcaga aagttggatg aggacgaaaa
    16741 ggggcaggcg aggtagacca cacaacaagg caccgataat tcggtgcctt taccgtatgc
    16801 tttatccgaa atatgtcagc catgctaact gcattgctga tatagggctg tttgcactgc
    16861 cggaccaagc ataattctgg ccattataat gaaactcaac aacgtatgag ccatcaccaa
    16921 tatttctcac aggcttaaat accggcttag atacaggtga agaaggcgcg ggcggttgct
    16981 gttcttctac gctctcttcc tcttcctctt cctctatctc aatttcgtcg tcatccagat
    17041 cgtcatcctc tgataattca tccgtctgtt caggtattgc ttccagctct tcttcgtcag
    17101 gcataacgat ttctggagaa tctattttca gctgccattg tccattttcg ccaacaaatt
    17161 gccccagagc atcagcagca aattccaggt atcgactaat cattgtaggg ctaaatttga
    17221 atgctcttag agtgctgttc gtgattttta tagaggggtc atgctctacc agctgcttaa
    17281 ctgtgttatg cagacgaacc ccggcatctc ctctggcgaa tcctggcatt tcgtcatcaa
    17341 gctgctgcaa tgcttccagc ctggtatttt cgtttccagt atttggcctc catgtctttg
    17401 agaagttggc cagcttgaat tgcttatagt gtaattgagt gttttcatcg tcatggccta
    17461 aaatttccat aaagaaaaca tcctcatcta cgtttttcca acgaggatct acgcggaaaa
    17521 acatttcata cgcaatacgg gcataaatag cacgactatc tttataaaca cggcggtcat
    17581 ctttgaaaaa tgatttgacc cacgggttaa aagcctttgc taatatggca ttaatccgtc
    17641 cgttttcaga gcgtgtgtcg ttatctccat aacctttaac gacatcatca aaatcagatg
    17701 ctgctgggca tgagcgaagt acatttatta aataaacaaa tgtttttgct tcgcataatg
    17761 tatatatttt tctggttata tcatcagaag ttcttttttt tgcttggcct aaaaaatcaa
    17821 cggtatactt accagaaaca gtaaatgtac cttgatacat aatctcaatc attcgtcttc
    17881 ctgacaatgc agcaagtgca aatgccaaag gagccatacc tgaacgagtg tttagattat
    17941 ttaatgtagc agggctattt ataatatcgt aaatagcttg catatattta gggtaatcaa
    18001 tgaccaccac gttacgtttc ttattgtgca gaacgtccgc ccatcgttgc tggatagatg
    18061 cgcgttcggc agggcttagt tgaagatgat agagaatctc atggttaact tttagctgat
    18121 tcaactcttc aagtaaggca gatccttgct gtagtaattt atataaataa tcgcgcttat
    18181 ctttccagtt atcgctagtt aaatcagata tggcgaattg ccactcttta tatttatttg
    18241 ataattttaa taacttatta tgacttttgt tatcaccaat tctgatatta gataaatctt
    18301 ctgataaagg gagaattaat tttaattttg cctgtaaagc tgacattctc tggcgaatat
    18361 cggcagcagg tagagaaagc catgacaata gctcctcatt atataaagga tacttttcag
    18421 ataggcgatg tatatttttt tcaaagctat gatgaagttt atcatcaaac cgttttctgg
    18481 ccctgctcat ataggcattg aatgtgttgg ctgtaattct tttttctaat cctttccccc
    18541 taaactttcg cttatcatta aataatgcat ttttaaattt taatgcaaca gcctttattt
    18601 tttttgtttt ttccccttga ggacggtcag agttatctat tgactcaaca tcgctaacaa
    18661 gtgagtttat taattcaccg atttttactt tgctcatctt tcctctcctg attatcagct
    18721 ctgattatcg taacacaatt tgagaataag ataactaata acgcgttaac ttagccacaa
    18781 actaacagaa cataatgacg gataataaac aaaaacacaa tatcggaacg ttaaaatttg
    18841 ttccttgact ttgttctaaa aatcagcaca taatccccca ttatacgcgc gtataatggg
    18901 gaattatgtt tcgttatgtt cttgattggt gttttcattt gatgtaatgt tctcagtttg
    18961 cgtactgttt tatttaacat ttgtttttat tctttattta tgttctttat tgtgtttgtg
    19021 ttattacttg ttttttatgt tctttatttg tgttcttatg ttggtaaggt tgggagaact
    19081 aatagagaaa acgtatctca ttgatgattc ttatcatgtt tctctagttt tatatgattt
    19141 cctttcttaa gaaaggtcat aaatatcaat tagttatgat tatattgttg ttcgttttat
    19201 gcatttcggg aggggaaaaa aagatgattt agctcaaata ttcgcctttg cgtttttcgt
    19261 tttgctttgc cttttttatg caaatttgct agaaataagc aaagatttaa ttattaagca
    19321 aagttacaag ctggagatga cagtatgtct ttgaaaaagt tattgcaaga ttgtgttacg
    19381 agaggtcaag aaatgaccca agcaattgct atcgctcaat tcggtgatga tagtcctgaa
    19441 gctcggcgta tcacccgccg atggggtata actgaagtag cagatttaat tggtgtaact
    19501 cctcaagcaa ttagagatgc tgagaagagc gggcgtttac ctgcacctga ctttgaaatg
    19561 agaggaagaa ttgaacgtcg tgctggttat accatagatc aaatcaacag tatgcgagca
    19621 gtttttggca acccaaacca acgaccacaa gataaaaacc ctgttgcgct tgccgttatg
    19681 tcacacaaag gcggcgttta taaaacatcg tctgcggtac atcaagctca atggttagca
    19741 ttacaaggtc atcgtgttct tttggttgaa ggtaacgatc ctcaaggcac ggcctctatg
    19801 tatcacggtt atgttcctga tttacatatc catgccgagg atactttact gccattttac
    19861 ttaggagaac gcgataacgc tgaatatgct ataaagccaa catgttggcc tgggctagac
    19921 attatcccta gctgcctagc tttacatcgt atagaaacag atctcatgca atatcatgct
    19981 cagggtaaat tgcctcatcc tccacacctt atgctgcgcg cagctattga atcagtttgg
    20041 gataactacg atgttatcgt tatagatagt gctccgaacc tcggtacagg aaccattaac
    20101 gttgtttgtg ccgctgacgt tattgttgtt gctacacctg cagaattatt tgattacgct
    20161 tctgttctac agtttttcac aatgcttctc gatctattgg aaagcgttga cctaggtgga
    20221 tttgagccaa ccgttcgctt acttctgact aaatatagtc tcactacagg gaatcaatca
    20281 cgatggatgg aagaacaaat aagaaataca tggggatcaa tggtattacg tcaggttgta
    20341 cgcgttaccg atgaagtagg taaaggccag ataaaaatga gaacagtatt tgaacaagca
    20401 gcaaaccaac gttcaacatt gaatgcatgg agaaatgctg tttctatatg ggaccctgtt
    20461 tgccgagaaa tttttgatga tctaataaaa cctcgctggg agaacgaata atgaaacagc
    20521 gctctcttct aaaaaatgct ccagatatta atcgcattat gagtaataat catcatgccc
    20581 ctgaacatca gcccgtatca cctatggttg gtgatttaaa acgtcaactg agttcattaa
    20641 ccggtaacag cataactttg ccagtatgcg gacgtaatgt taactttaag ttagagacaa
    20701 ttcccgcaga taaagttgaa atggcaacaa tggtatggct aggtaatgaa cgcgatcaag
    20761 atttattaaa tgaatcctct ctagccgatt taatcccatc ttttctaacg tctggacaac
    20821 aaaatccagc tttcgccaga aaagtatctg gaatcgttga agtcgctgac ggctcacgta
    20881 gaagaaaagc agcgatatta acaggatgtg actatcgcgt cttaattggt gatctcgacg
    20941 atgaacagat gcattggtta tcccaaattg gtaacgatta taggcctatt agtgcatacg
    21001 aaagaggaaa gagatactta cgcaagctaa atgactttga tggtaacgtt aaagcattag
    21061 ccgaggctga aaatatagat agaaatatta tcactcgctg tattaataca gctggtctac
    21121 caaaagatat attagctatc ttcaatcatc caggagaact gtcagctcgt gctggagatt
    21181 ctttgtttaa ggtttacaaa aaaaacatgg ctgccatgag caatgctgct catcatcttc
    21241 tggcaattaa aaaaaacggc gaggatttag aagcttcacg gattatacaa atattatctg
    21301 actctgttct ggttggcgac gaggaaaaaa caaaagacga gaaaaaatat ggggaaggga
    21361 tcacggctag atataaaggt aattttgtaa ctataaaaat tgatagccgc aaaatatcta
    21421 aagaaatact aagcagaatt gagttgttac tagaggaaca atctactgat aattaagaac
    21481 tatcccctgc attgcagggg atattgattt atggttgact tggccactcg atatctggag
    21541 atagtgatgt atcaacacgg ttcagcaaca cccgatactt cttccaggct tccagcaacg
    21601 agttttcttc ctccgttgca atttccagat ctgcagcatc ctgaagcggc gcaatatgct
    21661 cactggctac ctgcatcagg ttgttttttg tttcttccgc ctcccggatc cggaacagtt
    21721 tttctgcttc cgtatccttc acccaggctg tgccgttcca cttctgaaac tccccttccg
    21781 gggataacca ggtgacattt tccggtaatg ggccaagttc agaaataaat aacgcgtcgc
    21841 cggaagccac gtcatagacg gtttttcccc gatggtcttc aacaagatgc cacgatgcct
    21901 catcactgtt gaaaacagcc acgaagccag ccggaatatc tggcggtgca atatcggtac
    21961 tgtttgctgg cagacctgta tgaggcggaa tgtatgcgtc accttcacca ataaattcat
    22021 tagttccggc cagcagatta taaattgtta tggtccgtgg ttgttcactc attctgaatg
    22081 ccattatgca agcctcacaa tatagttaaa tgcgatgttt ttgacggtgt tttccgcgtt
    22141 accagcagcg ttaacggtga tggtgtgtcc atgtgagcca atcgcaaccg agtgcgtatg
    22201 agcaccaata ccgacagtat gcgcgtgtgc acctgcagat gctgctgtgc cggacagtga
    22261 gtggctatga ttaccatctg tactggtatt agctaaccac cccgtagaca tatctactga
    22321 gccttgtaca ccccaggtat tttgacctga gcttgtataa ccatattgat aagtatcttt
    22381 aaaaacactg gggttaaatc gacggccatc tctatggctg tgattaccag ctgcattcgt
    22441 gctgccactt aaactatggg tatgcgcacc agtgttattc gtggatttag tgccgtaatc
    22501 aaacgacgat gtggttttcg tccccaaatc cgtactggat gcgctggcgc tgtgggtgtg
    22561 cgatttaatg ccgtcctgtt cctgagacaa tacggcccga ccactggcag gttttccctt
    22621 aatcgtccag ccacgcatat cagggatcac gcctgacgga taagcggctg caagtttcgg
    22681 gtaagcagat ttgtcaaaag tctgcccctg catcagggca taaccagacg gaacggtatc
    22741 tgatggccac gggattggtg cgccgactgg gtagctttct ggtggaagat ttttcgaggt
    22801 ataaacttct gcccagtctt cctcaaaacc ataaccgtct cttgaagaac ggtagaacag
    22861 accaccattt ctgtaatgcg ccttcatctg caaggtccgg caacttccga ctccggtata
    22921 gaagttaacc agaatatagc tgtcgccaga gcgggtgaca ttgtaagcgc ctgattcggc
    22981 attccaggga acgccaccat ccgcatcggc atatgtatcc gttgcccttc tggcaaaagc
    23041 agccacatgc gcggcggtta aagtaatatc tttggaacca tcaaactcaa caccagaaac
    23101 cagtcttggc gtttgcagct ttgttgctgt taatgcatta ccgttcagac ttgcggacag
    23161 tttggttcca ataaccagtt cgccggttgc gttatcaata gcaaacggtc ttaatgtatt
    23221 ccagccacca taaacatcac cttgattggt aagcagcagg taagttttag cgccatcatt
    23281 acgccataat gcaccatact ccccacctat cattcgaatc tgattaccac cacgcgctac
    23341 aatttcgtct gtggcaaaaa gttttttgca cgacaagtta tcgttaacga ttaacgaatg
    23401 agactcataa aaaccacgcc cactcttaaa atcaaggata acgtccgccg cgatacattc
    23461 agtcgctgga tttgttgccc caaacttata ggtcgtatca ttaacaacga gatcagcacc
    23521 aggtgcggat attgacaggc catcttcgat aaacgcaaaa acagggaaag cagcgccatc
    23581 aacatagaac acagagcgca aatcatcgcc cttattactc atcattattg agtggatggc
    23641 tcgttcattg ttttgatatt gccagaacat tccataagca taacgccccc tgtcagtcca
    23701 gccaccaggc ataacaaatc cgttaaactc gcagttattc atcggatcgc ctgcggttcg
    23761 cgttgccgtg gtgataatga cccttgatgc cagttcgctt actgagccag cagaacgcat
    23821 aacaacaaca gggtaatatt ttccagatgt tgcacctgca ggagcgttaa cccgcacata
    23881 acgcatacca cgcttatcag caaagtctgt tttactgacc gcgttaatgt tgttcaggaa
    23941 gcgtccctta tcgggtatat cagcgccgtt ctggtctttc tgcagacgtt tctctgcatt
    24001 gtcataggct gattttactg cctttggcgt tgccgccagc gtttcagacg tactgttggt
    24061 cgcactgctg agctgtacta tccccttttt cgtcgtactt gcatcctcaa gcgccacggc
    24121 ggatgcaata tcctctgccc gtttagctgc tgtctcggcg cgcgttgccg cggattccgc
    24181 cgtacttttg ctctgagctg ccgccgtcgc actgccagct gcctctgtcg ccttcgtgga
    24241 tgctgtcgtg gcgctgccct tcgctgctga cgcctgtctg gtcgcctcat cttttgaagc
    24301 agacgcagat gatgccgatg acgccgccga actggctgac gatgcggctg ccgtttttga
    24361 ggattctgcg ctggtttccg acgctttcgc gttcgtttcg gatgtcttcg ctgcggaagc
    24421 tgacctcgct gctgccgtgg cctgttcagt ggcttcgcca gccttcgttg tggctgttga
    24481 agcggatgat gcggcgcttt ctgccgactt tccggcagcg gtggcactgg ctgaggcctg
    24541 cccggcattt gttgacgcgg cgctggcaga tgaggcagcc gctgtttttg agtctgctgc
    24601 tgcggaggca ctctgtgctg ctgccgtctc agaagactta gcgttcgtct cggacgtttt
    24661 tgccgccttc gcggaattgc ctgccgctgt tgccgaggag gctgcactac tggcgctcga
    24721 ggctgcgctc gtttctgatg attttgccgc ctcttttgaa gccgacgcat ctctggctga
    24781 ggtggcagct tctgacgctt tcgtggtcgc ggtggatgca gaagtggcgg ctgattgttg
    24841 tgacgctgaa gcattcgttt ctgacgtttt cgccgcaccg gcactggtag ccgccgcgct
    24901 ttttgaggac tctgcagcgg cagcactttt tgatgcttca gtggcctttg ttgatgccgt
    24961 tcctgcgctg gaagacgctg actgagccga cgacgcggcc tgtccggctg acgtgctggc
    25021 tgcgcgtgct gagtccgcag catcagccgc atgggttgcc gcctcacggg ctgatgtgct
    25081 ggcatcactg gctgacttct tcgcggctgc cgtgttctgt gccaccgcgg acgcgttacg
    25141 cgccacctct tccaccatca gttcaaaacg gcgcagtgcc tccggacggg catcatcctc
    25201 cgtcatggca ccgagaaaat cattcagcgt accgggttgg gaatcttcat acacggtgat
    25261 ggtcccggca tgtgacggcg ggaatccttc caccaacaga ataacgctgt actgaccgta
    25321 ctcaacgtcc atgctgtaac gcccggcttc atccggattt tctgaggcca gcgtgttcac
    25381 caccaccgtg gtgctgttac gttttgcttt cagctggatt gtgcagttct gtaccggttt
    25441 tcctgtgcca tctttcagta cacctgaaat ctttactgcc atattcaccc cacaaaaaag
    25501 cccgcctgaa ccggcgggct gtcataacac tgtgttacct ggctaatcag aacttataac
    25561 cgacacccat gatgaaaccg tcagtgcgcc agtcgccact gccggagcct tcataagcaa
    25621 tatcaatggc cacggattcg gtcgggttaa actgcacgcc agctccccac gccagagacg
    25681 tgttgctgtg gcgaccgtca tcacttccgg tcagcacgtc gtgccttttt cccttgttgt
    25741 cagttacgcg gagataatcc ccggaaaaag tcgacacacg gctgtaagcc acgcctgcca
    25801 tcgcatacgc gctgaaccat tcattcacgc gtacagacgg ccccgccatc acgctgaacc
    25861 agcggttacg cacggaatct tcatgccagc gggtatcgct gtaatgggtg agctggcgat
    25921 tcttgtctcc tgcatagctg aacgacgtca ccatccccag tgtgtccgta aactcataac
    25981 ggtatttcac gttaatcccg ttcagatcat cactgccggg aacgttcgtc cgggcatgaa
    26041 gataccccgc gctcagcgtg gactgatgtt cagacgccca tgcaggcgca ccggatacgg
    26101 ccagacaaat ggctgcggac aaaatggcgg cataaagttt acgcataatt acctctcgct
    26161 tttctgcaat aaaaaaggcg tcatttctga cgcccgttct gggttataaa attcagctga
    26221 tactgatacc tgctgtggat tttttcatca ccacaaccag cagatcgctg atactggttg
    26281 ttggtgtcca gttattcgct cctgatgaag atacggtgaa tgtcagtgtc agcgtcccct
    26341 gtccggcagg catatctata actgaggaaa atacgccctg agcatccgtc gtggactgat
    26401 taaaaatctc ctgaccattg cgggtcactc ttaaccggca ggttgaatac cagtatgact
    26461 gttggttatt actgttgaaa ttctcatgct taccaccgcg gaataacact ggcggtatca
    26521 tgacctgccg gtcaaatttc tgatcatcac tgattcttac cgtgatggtg ccactggcat
    26581 aactgttcgt gcgggggaaa gacttgctga ccgttttgac aatatcacct tcaatctggt
    26641 tggctgacag tttcccctta atctgacagt tctcattaat cgtgacgttg ttgagcgtcc
    26701 cggagttcgc attcacactg ccactgatat ccgcattttt agcggtcagc tttccgtccg
    26761 gtgtcaggga aaaggccgga ggattgccgc cgctggtaat ggtgggggcc gtcaggcgct
    26821 tcaggaacac gtcgttcatg aatatctggt tgccctgcgc cacaaacatc ggcgtttcat
    26881 tcccgtttgc cgggtcaata aacgcgatac ggttagcggc aaccagaaac tggctcagtt
    26941 tgccttcctc cgtgtcctcc atgctgaggc caatacccgc gacataatgt ttgccgtctt
    27001 tggtctgctc aattttgacg ccccacatgg cattccactt atcgttggcg tccttccact
    27061 ctttcgaaaa ctcctccagt ctgctggcgt tatcctccgt cagctcaaag ttttccagca
    27121 gttcctttcc gagatgcgtt ttattgatca accctttata aaaattcaga taaccttccg
    27181 catcatcgct cgcccgaccg acggcctcca caaatgccga tttaccaacg gtattcacgc
    27241 tgcggatata aaagtaataa tcataacccg gcttaatatt gctactggca gctatccagt
    27301 acagcgccgt gccaagatag cgggctgtgg tttcaacctg cctgatatcc gcaatccgct
    27361 tttccgagaa ccagaactca aactgcaccg tcgggtcata tacagccagt ttcgggaccg
    27421 ctgttatctg aaaataccct ggtatcagtt caatagtgac aggcgctgcc ggtgcggcaa
    27481 tccggaacga taccgatgca ggatcgccct gctgccccca cgcatttgcc gcccggactg
    27541 tcagcctgta gttccccagc gccagttgcg tgaagcggta tgtggtttcc gtcgtccggg
    27601 ccgtgctgac cagccgctca ctgccgtcat ccgctgccac ggtcaggcga agcataaagc
    27661 tcacgccctt caccaccttc ggcgtgtccc agcgcgccag cacctgatat tccccgctgt
    27721 ctgcggtgac ttcggcagtc aggtgctgca ccgcgggcgg cgtgacgcca ttcaccgtgc
    27781 cgctctggtc gccgtcaaag tgcgccccgt tatccacgat ggcttctttt tcgggtacat
    27841 gctgcacggc ggtgatggca tacgtgccgt cgtcgttctc acggatactc acgcagcgga
    27901 acaggcgctg gcgcaacgtc ggcaacttca gcccccacac gctgtattct gcaacgccgt
    27961 caggaacacg gctcactttt accttcacgc cgtcggtgac ggactggacc tccacgctga
    28021 ccggattgcc acttccgtca accaggctta tcagcgtggt accggaggat ggcagcgtga
    28081 tttcacggtc gagcgtcagc gtccgggtct ggctgtttac cgccagcacg cgcccgccga
    28141 tgctgatacc ggcatagtca tcatcgcaga tttcaatgac atcgcccggt acatggcgaa
    28201 gcccttcggc acccacgctg aagtccacgg tctgcgtttc cagcagttcc gttttaatca
    28261 gccacagccc ggcgcggtgt gcctgccccc gactggtaca gccaaaggca tccatcttcg
    28321 tgacattacg accgtaacgg gcaatggcct gcgtgtcctc cacaagctct gttgccgttt
    28381 cccagccgtt atccgggtca atccagttca cctcaacggc attatggcgg tccttcaggg
    28441 cgctgaaact gtagcggaac ggcgcgccat catccggcat caccacatta ctgcggttat
    28501 aggtccacac cttatccgac ggtcggtcct gcacgaacgt cagcgtctgc ccgttccata
    28561 ccggcataca gcgcatcgcc gagcagaaat cactgagcac atcccacgcc ttgcgctgtg
    28621 tggtcagcca ggcattacag gtgatgcgcg gctccgtgct gccaaagccg tccggcaccg
    28681 actggtcaca gtactggccg atgacataca gcgcccattt atccacatct gccgcaccaa
    28741 gacgtttccc catgccatag cgcggatggg tcagcatatc ccacagacac caggccatgt
    28801 tgttgctgta tgccggtttt aacgttccgt cccagatacc gctgtattgc cgcgtctgcg
    28861 gattatagtt cgacggcacc tgcagaatgc gcccgcgaag atgataatta cggctcacct
    28921 gctggctgcc gaactgctcc gaatccacct gtacgccgac cagtgccgtg ttcgggtagc
    28981 actgtttcac atcgatgatt tcggtgtatg acgaccagag cgttttgttc tgcagctggt
    29041 ctgtggtgct gtccggcgtc attctgcgca tccggatatt aaacgggcgc ggcggcaggt
    29101 tatccaccac caccgaggcc agatactgcg aggtggtttt gcccttaatg gtgatgtctt
    29161 tttccgtcac ccagccaccg ttacgctgta tctgaaccag caggcgaact tcagacggat
    29221 tcctgtctcc ctttgaggtg gtttccacca gtgcctgtac accgaaggta aaacgcagtc
    29281 ggtcaatgtt tgccgacgtg atggtccggg tgatcggcgt gtcatatttc acttccgtac
    29341 ccagcaccgt ctcggagccg gaggattcaa atccctccgg cggtgactgc tcctgctcac
    29401 ctgcccggaa caccaccgtg acgccggaga tgttggtatt cccctcactg tccagcaccg
    29461 gcgtactgtt cagcagcacg ctttttaatc catccaccgg accttcaacc ggcccttcgc
    29521 tgatggcatc gatcacactc agcagctgcg tggacttcag gttgtccttc gcttcgcgcg
    29581 gggtatgccc cttactgctg cctttaccca ttcgtcatgc tccataaacg ataaaaccgc
    29641 ccggaggcga tttcacataa aacattttgc atcagcgacc aatcaccaca acctgaccac
    29701 cgtccccttc gtctgccgtg ctgatctcct gagataccac acgtgacccc acgcgcattt
    29761 caccatacag aacaggcata acattgccct gggcaaccat gttatccagt gaggagaaat
    29821 agatgttctg cttaccgtta tccgttgtct gtgtgcgggg agttctggct ttcggtgcca
    29881 gcatctgagc cacaccgccg agcaccatac tggcaccgag agaaaacaga atgccggtca
    29941 taccaccggc accaatggca gccccccatg ctgcaagggt ggctccggcg gtaaagaatg
    30001 atccggcaat ggcggctgct cccaggacaa tctggaatac accacctgac ttggccccgg
    30061 cgactctggg aacaatatga atcacagcgc cgtcaggcag agcctcatgt aactgcgccg
    30121 ttaatccgga cgtgctgaca tcctgcccgg caatacgcac ctgataccag ccgtcgctca
    30181 gcttctgacg aaacgcaggg agctgtgtgg ccatcgcccg gatggcttcg gcacccgttt
    30241 tcacacgaag gtcgatgcgg cggccaaatc gttgcaaatc cccgtaaagg cagatgcgtg
    30301 ccatgcccgg tgacgccaga gggagtgtgt gcgtcgctgc catttgtcgg tatacctctc
    30361 tcgtttgctc agttgttcag gaatatggtg cagcagctcg ccgtcaccac agtaaatggc
    30421 ggcatgattc ggcaccgatg aaccaaaaca gcacagcagc acatcgcccg gctgcgccgc
    30481 tgacaacggc acctgataca gccctgtggc ctccagatta tccagataga gattctgacc
    30541 gtgacgccac cagtcatcct cacgatgaaa atccggcatc tcaatccccg ccagatggta
    30601 agcatcccgg aacagcgtgt aacagtccgt caccccgtgc tcaaagcgcc gcccggtaag
    30661 atgtggcaca cagcggaact tgtgaatcgc cccccggcag accagccacc acggcaaatc
    30721 actctgcacc tgcagccgcc ggtcggcctc actcagccag ggcagaccac cggggtggct
    30781 gtggaccagt gccacaatct caccctgcat ctctgcctgc agccagtctt ccggcgacat
    30841 acggaaatac gcctccggct caccggagat attcacgcag gggaaatatc tttccccctc
    30901 cggcgttctt accacgaagc cgcacgactc cgctggagca catcgccggg cgtgcgccag
    30961 aatcgctgat tctgtctctg tcatgggatt tactgcgaaa gtttgttaat ggaaaggaag
    31021 ccgccaaagt tgccgacgtt attgcgaaac ttacagccac tcaggcattt gctgcattta
    31081 tccttcgtga tgtcggacgt tggctggtca tattcatccg cgaccgccgg accgtgataa
    31141 ccgcactcat cgccgcgata ggtccaggtg caggtgttgg ccagcatgat acgtcccgga
    31201 aaaacagcgc catctgtttc cgtcggcgtg gacagtacaa aagaggcact caccgcgctc
    31261 agttcgctgc actgctcaat gcgccagcgg ctgatcacct cctgctccgg atcggcgtca
    31321 ctgtttccgt tgacgaagtt caccgcatcc agaaaacggg cgtaaacctt acgccggacc
    31381 accgttccgc cgaccagact ctgtagatct tccgccatcc cggtgaccat accgtacagg
    31441 ttagaaacca tcagcgtggg gcgcgtactg gtgcctttgc cattcagttc aaaaccactc
    31501 ccctgaatgg gatacggctg atactgtcgc ccctgccagg tgaccggctc acctttttcg
    31561 ttctgctcat tacagaaaaa ataacgttct ccaccgacct ctgtcagatc gatttcccag
    31621 agcaccacgc tggccgactg ctccgcacgg gtgcattcat tcagtgtttc ctgccggata
    31681 tcctgcatca gttcaccacc tgttcaaact ctgcgctgaa ctcaacacgc agcatactga
    31741 cccgcgacga ccattttgcg caggtcacct ttatctgccg ccactcataa ggcggcgtcc
    31801 acagaaaggc tttccagccc ccgtgctctt ccagaaacga ctccagtacc atggcctcct
    31861 cacgggggac agaaagcgtc acgctgtacg ttttcaggtt ggcattcagc ccggcaggcg
    31921 ctcgctggga atagccatca ccaaagcgca cctttcttac agaaggggcc gaagccacat
    31981 ccataccggg tttcactttc cagcggaagg ttttcatcgt ccacctccgg agaacaggcc
    32041 accatcacgc atctgtgtct gaatttcatc acgggcaccc ttgcgggcca tgtcatacac
    32101 ggccttcatc atctgtggac ctggcagacc attcgtaccg tcgttctgaa tcaccacgtg
    32161 attgttctga ttaaaattaa tgccttcagc ccgccgcatc tgcgccggac ttccggcacc
    32221 gcccacataa ccaccttccg catagccgcg catcagccgg tagagattcc ccacgccaat
    32281 ccggctggtt gcctccttcg tgaagacaaa ttcaccacgg tgaacaatcc ccgctggctc
    32341 atatttgccg ccggttcccg taaatccccc ggttgcaaaa tggaatttcg ccgcagcggc
    32401 ctgaatggct gtaccacctg acgcggatgc gccgccacca acagccccgc caatggcgct
    32461 gccgatactc ccgacaatcc ccaccattgc ctgcttaagc agaatttctg tcatcatgga
    32521 cagcacggag cgggtgaagc tgcgccagtt ctgttcactg ccggtcagca tcgccgccat
    32581 attctgtgca ataccatcaa aggtctgcgt ggctgcactt ttaacctgtg acatactgtc
    32641 cgtggcgctc tcttcccact cactccagcc ggacttcagg cctgccatcc agctcccgcg
    32701 aagctggtct tcagccgccc aggtcttttt ctgctctgac atgacgttat tcagcgccag
    32761 aggattatcg ccatactgtt ccttcaggcg ctgttccgtg gcttcccgcg ctgcctgccg
    32821 gtcagtcagc ccccggtttt tcgcatcaat ggctgcccgt tttgcccgtt gctgctgtgc
    32881 gaatttatcc gcctgctgcg ccagcgcatt caggtgctcc tgatacgtaa ccttgtcacc
    32941 aagtgcagcc agctggcgtt tgtactccag cgtctcgtct ttatgcgcca gcagggattt
    33001 ctcctgtgcg gacagctggc gacgttgtgc cgcctcctcc agtaccgcga actgactttc
    33061 tgccttccac aaatcccggc gctgctggct gattttctca ttcgctccgg catgcttctc
    33121 cagcgtccgg agttctgcct gaagcgtcag cagggccgca tgagcactgt cttcctgacg
    33181 atcgcccgca tacaccttca cgccggactg tttcggcttt ttcagcgtcg cttcataatc
    33241 ctttttcgcc gccgccatca gcgtgttgta atctgcctgc aggatttttc cgtctttcag
    33301 tgccttgttc agttcttcct gacgggcggt atatttctcc agcggcgtct gcagccgttc
    33361 gtaagccttc tgcgcctctt cggtatattt cagccgtgac gcttcggtat cgctctgctg
    33421 ctgcgcattt ttgtcctgtt gacgctgttg atctaccctc ttttgggctg catcccgctc
    33481 caggcgtttt ttttcacgat catcccagta acgcgcccgt gcttcatcgt taacgaaata
    33541 atcatccttg cgcagattcc agatgtcgtc tgctttctta aacgcagcct ctgccttaat
    33601 cagcatctcc tgcgcggtat caggacgacc aatatccagc accgcatccc acatggattt
    33661 gaatgcacgt gcagtcctgt ctgcccaggt ctccagcgtg cccatgttct ctttcaggcg
    33721 gcgggtctgg tcatcaaacc ctttcgttgc ggcctcgttc gccgcctgca atgccccggc
    33781 ttcatcgccg gaacgctgca actgagcaac atacgcaatc tgctccgccg tcacgttatg
    33841 gaactggcgt gccatcgccg tcagccccga cgtcgggtct gtggtcagct tcccgaaggc
    33901 ttcagcgacc ttgtccacct ccacgccgga tgcagaggag aaacgcgcca cactctggct
    33961 gatggacgca atctgagcct caccgcttac ccccgcctta accagtgcgc tgagtgactc
    34021 gctggtctgg ttaaacgtca gccctgccgc ctgcccggct ctggacagga ccagcatacg
    34081 atctgccgtc agtcccgcct gattgccgga aaggaccagc gttttgttga aatcggacag
    34141 ggttgagttg ccctgatacc aggcatacgc cagcgcaccg gtcgccaccg ccagcgaggt
    34201 ggcccccacc atcggcaggg tgatcgcacc ggcaagcccc ctgaacatgg ggatcatccc
    34261 gccgaaggag tccttaacct gaccaccctg ttgcagcagg atcagccacg gactttgccc
    34321 gcctgcaagc tgcgtggcca cgtcggtgaa ctgtgcaggc agcatacgca tggcggcttt
    34381 atactgcccg acggaaatcc ccgctttctg tgcagccagc gcctgtcggc tcagcgactg
    34441 ttcaacaact gccgctgttt ttttcgcatc actttccgta ccggaaaaat gacgcctgac
    34501 tctggccatc tgctcgtcaa atctggccgc atccagactc aaatcaacga ccagatcgcc
    34561 taccggttca gccataccgg actcctcctg cgatcccttc tgatactgtc atcagcatta
    34621 cgtcatcctc cgtcatgtcc gccacatccg gggaagtagg gataacttca ttcccgtccg
    34681 ggccaaagcg gacacctccg gcaagccctg ccgctttctg catcagcaca tcatcttcag
    34741 gctcttcgtc agcctcacgc cggttcagca gactgaaatc cagcggatgc atatccggat
    34801 cgctgaaaaa caggctgagc acggtgtacg tcagcccgga aaagtgcata tccagcagaa
    34861 catcatgaaa ataatgggta ctgtaaaagc ggtgccagtc ggcatactcc gtggatgaca
    34921 tcccggcaag catggcacgc cagtcgggtc gccccatttc gcgcgccagt ttcagggcaa
    34981 aactcagctc accgtcgaac actttcccgc agaaacaggc tctgcagacc cggcgtcctc
    35041 tgcctgttca ggggcatcat tcaccacaaa ctcatacata ccggacagcc ggtacaccac
    35101 gttttcagca tgagaaattg cctccgtggg ccaggtggta agcacttcct gctcaatctg
    35161 tttaacggct tcattcatgg aaggctgctt tgtcttctgc ggatggttat gccacaggga
    35221 catcgccacc acaaaagcac cggttctgat ggcgtcttcc acagtaaact tccggttgct
    35281 gtctgactcc gcctgttctg cctgccgttt catcagggcg agatgctcaa tacgctgcag
    35341 ggctgacagt tcagaaagcg tgacggtcac accgttatgt tcaaatgatt cggttttcag
    35401 gaacatcgct gactctccgg attaactggc ggtgacggta atttctgcaa ccgcagcaaa
    35461 ctcaccatta ccggatacaa ccggaatgtt gaccttgcct gcagcaacac ctttcacggt
    35521 gatggtcata ccactgaccg acacggtggc ttttgtttta tccgctgaca ccgcacggaa
    35581 gctcttgtcg gttgcgcctt ccggctggaa tgccacggtc agcgtggtgc tctgcccttt
    35641 caccaccgaa gtgctggcag gcgtcacggt catgccggtt gccgctgtta ccgtgctgcg
    35701 atcttctgcc atcgacggac gtcccacgtt ggtgactttc accgtacggg tgatcacttc
    35761 cttcgccgtc accgccttac cgatactgct gacccagccg cggaacacat cgaccgtgcc
    35821 gttcgggaag cggattttat aggcacgggt atccccttca ttaaaccacg ccagcagcgc
    35881 ctgctgcccc tgctctccgg gcatccacgc cagcgtgaag ctggtatctc cggcagattt
    35941 ctgcccctgc ccggtcgcag tccagtccgc atcttcatca tcgagataac tgtcgtcata
    36001 ggactcagcg gtcagttcgc cgggcgtcag gtctttaact ttagccagac gcgaccagtc
    36061 aacgtctgaa agcgggttcg cataagggtc gccgctcccg ttataaaccc acagggtggt
    36121 tccggcacct ttcaccggta ttgctggatt tggtacaggc atatcgtcct cacatttcat
    36181 aggtaatgac atacgtcaga tcggctgaac tccacaggcc cgcatcatcg tcgcgccggt
    36241 agtcatagcc actggccacc atactggtga tcaaatctga cagtgccggg atatcgctca
    36301 tcaccggata aatccgggac tccatccacg catccagctc tgaatccggc acctgagcag
    36361 gcaggaaaac ttcgatatgc agctccgcct gccaggtatc gctgtccagc tcttcgcccg
    36421 tgtattcagc gccggtgaga taaacggcaa ctgccggaaa atccgcctca tcaaaaacag
    36481 cggggcgacc atcaaaaaac gtcgccccgg tgtcatgctt ctccagtgca tccagtacgg
    36541 ctgcacggag ttcagtatgt ttcatcgctt tattaccatc ctcagttgat gctgcagcgc
    36601 atagcccagc tctttcggaa gacgctcacg ccgtatccgc tcaatattct gtttaaacgc
    36661 cgtggtcagc ggcaccgcca tcggaatttt caccacatca atggggtaac gatttttccc
    36721 ggccacacgc tgcataacat gccagcggcc atttttcagt tgctgaataa acgcgccggg
    36781 aatacgacgg tttcccacca caagcacgct gccgccacct ttcagggctg aacgctgccc
    36841 ctttttacga cgcctgcgtc ggggcaggac aatccgcgcg ttacccagct ttattacggg
    36901 caaatccccc cggttaacct tgattctggc ctgcggattt ttgaccgtgg cccttttcag
    36961 cctggccctt tcctttacca gtttccggcg tacctttgtc tcacgggcaa cctgtgacgc
    37021 cgactgcgat atcgcggatg acgcaacgcg gttaatggcc attgcggcgg caccgggaac
    37081 cgccgttttg ctgatacggc tgaggttttc aacggcctgc tcaagacctt ttatgaccat
    37141 acatccccct ttcaacgccg acggttaacg gcaggcggaa cgccacgccc aagccagaga
    37201 tgacagcttc cgccatcatc cggcgatacc cggtctatcc agaagttttc ctcaccgatg
    37261 gtcagcgtgt ctccacgccg cagctgccgc acctcatcag tccggacaaa cagggacggg
    37321 ctggagcctt caacgcgcac gccctgtccg gcatagctga tattttcagg gtcatcaaaa
    37381 acaccacgta ttactgcgcc ggactgctca ccggatgtca tggtggctga cgttcccatg
    37441 tacccgcgta tcgtttcatc ggcgcgggca atggcagcat cgaacaggtt atcgaaatca
    37501 gccacagcgc ctcccgttat tgcattctgg ccaggccacg ttctgtcatt tcagctgcca
    37561 caccggcaga gacacggaac gccgttcccg gcagcacaaa tgccacagcc tcatcccgcg
    37621 tggcgtgaag tgcatcagta tgcagcgtca ccagtgccac aaccgtgacc agatcagccg
    37681 tatcagtcac ggtatccggc tgcgctgata caacctcatt ttcatgtccg gtcagcgcat
    37741 tttccgggct gacagatgtg tcctgaccgg cagcgtcatc cgtgccatca agctcctctt
    37801 ccagctctgc cacacggagt gccagttctt ctttcgtccc cgtcaggctg acatcacggt
    37861 tcagttgctc acccagcacc tgaagacggg caatcagttc atctttcgtc atggactcct
    37921 ccacagagag aaaatggccc cgaagggcca tgattacgcc agttgtacgg acacgaacgc
    37981 atcagggtca gccagcagca tcagcggtgc tgactgaatc atggtgaact cacgcgccgg
    38041 atcgccggtg gtcacccagt ttttcgggta gcgggcagag gcgttaatac cttcgcgctg
    38101 tgcgtccgca tcctgaatgc agccataggt gcgcagaccg cgtgcctgag tgttccccag
    38161 caccatcgtg ttgtccggaa ggaagttctt tttgacgtcg ttttccacat actgtccgga
    38221 atacacgacg atcgccgtat cgccatacat ccccttatag gacaccgctt cgcccaggtc
    38281 ttttaccgct gtctccagct cggaattaga gccgcgacgg gtatccagct tctccttgac
    38341 ggctttgaag gaacggaaca gcgcccagcc tttcggatca aacacgatga tattcaccac
    38401 accgctggcg ttcagcgcgt aggcttcgat atcgtcggtc gggtcatacg tggacttgtc
    38461 acgcttgctc cactccgtac cgccggactg cgtgatgttg ttcgccacac tgcggcccat
    38521 atccacttca accggatcga aggcttcacc ggtcatggtg tatttgccct taagcacggc
    38581 agaaacggcc tgcatctctt cgacctgagc aatggccagc tcttcgtctc gcatgttctg
    38641 caggatgatg cgacggcggc ggtaagccgg gtccgccaga ttctgtggat cttcatccgg
    38701 cagacgacgc agggtcatct gcggattcac ctcatgcttg ggtttgacat atcccggtgt
    38761 aaattcagag gtggagccgc cacgggagcg gataacctca ccggaaacaa tcggcgaaac
    38821 gtacagcgcc atgtttacca gtcccggaat ttgtgagaga tagactttct ccgtggtgaa
    38881 gggatagctt tcacggaaaa agagacgcag aaacagcgga tcaaacttaa atttctgctc
    38941 atttgccgcc agcagctggg cggttgtgta catcgacata aaaaaatccc gtaaaaaaag
    39001 ccgcacaggc ggcctttagt gatgaagggt caggttaaac gatgctgatt gccgttccgg
    39061 caaacgcggt ccgttttttc gtctcgtcac tggcagcctc cggccagagc acatcctcat
    39121 aacggaacgt gccggacttg tagaacgtca gcgtggtgct ggtctggtca gcagcaacag
    39181 ccagaattcc aacggcagca ccgtcggtgg tgccatccca cgcaaccagc ttacgggtgg
    39241 cggtgtccag catcagcgga gtcattgcag gcgctttcgc actcaatccg ccgggcgcgg
    39301 ttgccgtatg tgccgggtca ctgttgccca gcggctggta atgggtaaag atttctttgc
    39361 tcgtcataaa catcccttac actggtgtgt tcagcaaatc gttaacggca tcagatgccg
    39421 ggttacctgc agccagcggt gccggtgcac cctgcatcag acgatccagc gcagtgtcac
    39481 tgcgcgcctg tgcactctgt ggtgctgcgg ccagaatgcg gcgggccgtt tccacggtca
    39541 taccgggggt ttcggccagc acgcgtgcct gttcttcgcg tccgtgagcc tcctcacagt
    39601 tgaggatccc cataatgcgg ctgttttctg ccgcaaccgc tgcggtgatc tgcgcgttca
    39661 cgtccggctg cgcagcgctg gcgttctcgc cctccgtcgc ttgcaccacg ccagtaacgt
    39721 cagcctgcga agcagtggct gaaacagttg ttgattgagt ctctttggtc attcgccctc
    39781 ctgagagacg ggatttacgt gcatccagtg catcacgcat gacggtgatc gcatcggtac
    39841 tgttaacaag ttcatcagcc agtccggcat caatggcctc ctgaccgctg tacactgcag
    39901 cctcggtatc cagcacagcc tgcacggaca ggccggtata tgccgacacc ttctgtgcaa
    39961 acatccggcg ggttgcatcc atccgggact gcagtgtttc ccggacatca tccggtagat
    40021 ggctgtaggg gttgccatcc accttatggc tgccgctgta aatcagcgtg atttccacgc
    40081 cctgtttctc cagcgcagca ccgtaattac tgtgagccat catgacgccg atggagcctg
    40141 tccgggcggt ctgcgtgacc agacgccggg aggcggcact ggcaagcagc tgacctgcgc
    40201 tgcagttcat gtcattggcc agcgcccata ccggctttat gtcacgcaca cgggcgatga
    40261 tgtcagcgca gtcaaatgcc cctgccacca ttccgccggg cgtgtccata tccagcagaa
    40321 tgccgtccac catcgggtcg ctggcagcct gttgcagacg ggcgataatg ccgttgtaac
    40381 cggtcatccc cgaatacggc tgcagcgccc gcgtccggct gaccagcgtg ccggaaaccg
    40441 gcagcacggc gatgccgttc atgacctgat aactgcgggc ctgtcgtggt ccgtcatcat
    40501 taacggataa cgccagcgtc gcgggtgcct ctccggcagt caggctgtca ccggacaccg
    40561 catccgtcag gcggctgatc ccaagctggc ctgcaagcgc acaaaagaaa acccgcgcat
    40621 aggcgggttc aagcataagc ggctcattaa aggccatgct ggcaatatgc gggagattac
    40681 gcagctctgc tgtcactctt ctcctcctct gttgattgtc gcagtccgga ttcaaatgcc
    40741 gcagccgccc aggcgggcgg tttaagaccg gctgcacggc gctccatcgt ttcacggacc
    40801 tgctgggcaa aaatttcctg atagtcgtca ccgcgttttg cgcactcttt ctcgtaggta
    40861 ctcagtccgg cctctatcag catcaccgct tcctgtactt ctttcagacc atcgatggcc
    40921 atacgaccgg agcctatcca gtcacagttc ccccaggcac ttcgggcttc ctgaaaactg
    40981 aagcgcgctt ttgaaggtaa cgtcaccacg cggcgagcga tggcctcttc cagccagcac
    41041 agaaacatct ggctcgcctg acgggatgcg acgaattttc gccgccccat aaagtacgcc
    41101 cacgactcgt tcgcactggc ccgtgccgtg gagtagctca tctgagcgta attccgggaa
    41161 agctgctcat acgagacacc cagcccggca gcgatatacc gcaacagtga ctgctcaaac
    41221 acggagtagc cgttatccgt gtcctgagcc gtctgcaggt tcagtgagtc ccccggcatc
    41281 aggtgcggca cttttgcgcc tcccagccgg accggtgctg cggcgtaata cgcggcaatt
    41341 tcaccaatcc agccggtcag cctgtcccgc tgctccttac tgttcgcgcc aagaataaaa
    41401 tccatcgctg actgcgtatc cagctcactc tcgatggtgg cggcatacat cgccttcaca
    41461 atggcgctct gcagctgcgt gttctgcagc gtgtcgagca tcttcatctg ctccatcacg
    41521 ctgtaaaaca catttgcacc gcgggtctgc ccgtcctcca cgggttcaaa gacgtgaatg
    41581 aacgaagcac gaccgcccgg taactcacgg ggtatccatg tccatttctg cggcatccag
    41641 ccaggatagc cgtcctcact gacgtaatat cccagcgccg caccgctgtc attaatctgc
    41701 acaccggcac ggcagttccg gctgtctccg gtattgttcg ggttgctgat gcgcttcggg
    41761 ctgaccatcc ggaactgtgt ccggaaaagc cgcgacgaac tggtatccca ggtggcctga
    41821 acgaacagct caccgttaaa ggcgtgcatt gccacacctt cccgaatcat catggtaaac
    41881 gtgcgttttc gctcaacgtc aatgcagcag cagtcatcct cggcaaactc tttccatgcc
    41941 gcttcaacct cgcgggaaaa ggcacgggct tcttcctccc cgatgcccag atagcgccag
    42001 cttgggcgat gactgagtcg gaaaaaagac ccgacgatat gatcctgatg cagctggatg
    42061 gcgttggcgg catagccgtt attgcgtacc agatcgtctg cgcgggcatt gccacgggta
    42121 aagttgggca gcagggctgc atccacactt tcactcggtg ggttccacgc ccgcaactgc
    42181 ccaccaaatc cgctgccacc gccgtgataa ccggcatatt cacgcagcga tgtcatgccg
    42241 tccggcccca gaagggtggg aatggtggac gttttcatac ataaaatcct gcaggtcccc
    42301 tgcgtcgctg tgtcatgccg gtctgcactt ccagctccgc aatgtatttt ttcaggtcag
    42361 acacggaagt ggccgtaaac tccactctcc gtccgtcttt ctgtaccgtt gccacccgtt
    42421 ttcctgtcat caggtcatgc agtgccgcac gggcagcggc aagttcttcc tgtcgcgtca
    42481 ttcatcctct ccggataagg cacgggcgta atctgccagt gttttcttgt tggttgctgc
    42541 actatcctct tcctgcagac tcgccagcag cgcactgaga tccagctgcc agcgggaaat
    42601 actgatacgc agcgccgcca gcgcataaac gaagcagtcg agtgcttcat tgcgtcgctt
    42661 tttgctgtcc cacagtattt ttttcctgcc atccacccat ttttcgacct gctcttcagc
    42721 cgtcagctgc tgcgcttcgg tcagatcaaa aatatccggg ttattcggga agtgaacggc
    42781 accgggaagc ggttcatccc cttccggcgt cagtgtgaag cggttataaa tctgctcttt
    42841 cgcggtatcc gtaccgattt cggtaaggta aaccccgttt ttgtttcgct tacgtggcat
    42901 gctggccacc ggctttccgt agacggatgc ccctttaatg gggatcaccc ggaacagccc
    42961 atgttttttc gagcgttcat acacaatggt cgggtcaatc ccgccaatat cccagcagat
    43021 acgggatatc gacatttctg caccattccg gcgggtatag gttttattga tggcctcatc
    43081 cacacgcagc agcgtctgtt catcgtcgtg gcggcccata ataatctgcc ggtcaatcag
    43141 ccagctttcc tcacccggcc cccatcccca tacgcgcatt tcgtagcggt ccagctggga
    43201 gtcgataccg gcggtcaggt aagccacacg atcaggaacg ggcgctgaat aatgctcttt
    43261 ccgctctgcc atcacttcag catccggacg ttcgccgatt ttcgcttccc acgtctcacc
    43321 gagcgtggtg ttcacgaagg ttttacgttt tcccgtatcc cctttcgtct tcatccagtc
    43381 tttgacaatc tgcacccagg tggtgaacgg gctgtacgct gtccagatgt gaaaggtcac
    43441 actgtcaggc ggttcaatct cttcaccgga tgacgaaaac cagagaatgc catcacgggt
    43501 ccagatcccg gtcttttcgc agatataacg ggcatcagtg aagtccagct cctgctgacg
    43561 gatgacgcag gcattatgct cgcagagata aaacacgctg gcgggatcat ccggcgtcca
    43621 tttgaggcca aacggtgtct ctttgtcgcc aaatttaaga tactgctcct ccccgcagtg
    43681 cgggcaggca acatgaaaac gcataaaatg cggggattca ctggctgcac gctcaatctg
    43741 gcaggttcct ctcactttgg gcgtggagcc acggatggac tttggccaga ccgagccttc
    43801 aatacgcttg tcacccagga acgtcggaga gccttcctgt tcaatatcat catcaaaagc
    43861 agcaagttca tcataacccg ccacatccac cgacttttca cggtagtttt ttgccgcttt
    43921 accgcccagg caccagaaac cacgaccatt ggtgaaacgc ttcatggtga gcgtgttatc
    43981 ccggtgcttt ttgccatacc acggggccag cgccagcagc gacggaatat cacggatggt
    44041 cggctcgacg tgggttttca taaagttttc ggcatcacca tccgtcggca accagataag
    44101 ggtgttacgc tgcttatgct ctatgaagta ggcataaaca cccagcagca ttttggaata
    44161 accgacacgg gcagacttca ccacattcac ctcacggatg tagtcgctgc ccatcgcatt
    44221 catgatggcc cgctgaaagg gcagtgtttc ccagcgccct tcctggtatg cggattcttt
    44281 cgggagatag taattagcat ccgcccattc aacggcggtc tgtggctccg gcctgaacag
    44341 ggcacgaagc ccggcgcgga caaaatgccg cagcctgtca acctgactgt tcgatatatt
    44401 cactcagcaa ccccggtatc agttcatcca gcgcggctgc tttgttcatg gctttgatga
    44461 tatcccgttt caggaaatca atatgccggt tatccagctc aggaaaacgt cgctgcaccg
    44521 acagaggaat accgtcgaga atactggata tttcccttgc tatacgcgac agcacgaaag
    44581 tacagaatgc ggtttccacc acttcagccg attctttggc atttttcagt tcctgcgccg
    44641 tcgcctgagc acgagtcagg cgatggcgct caaattcaag tgttcctggg tgaagatctg
    44701 cctcgctggc cagccgcagt tcttcaacct cccggcgcag cttttcgttc tcaatttcag
    44761 catccctttc ggcataccat tttatgacgg cggcagaatc ataaagcacc tcattaccct
    44821 tgccaccgcc tcgcaaaacg ggcattccct gctcctgcca gttctgaatg gtacggatac
    44881 tcgcaccgaa aatatcagcc agctgctttt tgttgacttc cattgttcat tccacggaca
    44941 aaaacagaga aaggaaacga caaaggccaa aaagcccgtt ttcagcatct gtcgtttcct
    45001 ttcttttcag ggggtgtttt aaataaaaac atgaagttac ggcgaagaag aacggaaacg
    45061 ccttaaaccg gaaaattttc ataaatagcg aaaacccgcg cgccttccgc cccgtagcct
    45121 gccggatcgc cggaaaggac ccgcaggcgt tctggtttat tcgcaatgaa ttcaatgtct
    45181 gacagaccag cgtcgtgcga ccacggtcgc acgctcctga atacatgccc tgtttcttcc
    45241 acccccgcac agagctggcg agcatggggg acaaacccgc gaatcattag cgctgtaaaa
    45301 acccggtatg catcgttttt gattatttcc acacactcgc gcagaggagt tccccgtcag
    45361 gctacggtca tagttaatgc ggaaatacgg cgacgatgca gcgcataatg tgtcaggctt
    45421 gaataccttt atccgttaaa agggatatta gttaagttat cccgtgtagg gtataagcca
    45481 ttatcaaagc cactcagtag cgaatggctt ttgtaatggg ctatttaatt agtgatgctg
    45541 cgtattcgat tagatataac ttaggtgcca gccagatttt taaccatgtc atattggcta
    45601 ctatagaaat aataaaactt aaccatagaa taaaaactgc tactaatggt aagacaaaag
    45661 cacttaacct accgtcactt tcccaaatca tagtcggttt gtatttaggc tgtccattat
    45721 cacatgagcg tccgtcatta tcgaacttac caatttcaac ttttttgcac tgtttcttca
    45781 taaaccaaat aaccagcggg attgttgaaa tggctattaa tgttttaatc agactatcaa
    45841 ctatattcca taccagcaac tgatgaacaa catccggaat ctgagcctga ctgaatgcaa
    45901 tggcggaatc aattccatcg ctggcattgt tcaataaatc aacaagaatt ttctttgctt
    45961 cttcttgcat gtatatatcc tgtaagttgt aagcctgttc gaaaaaataa acaactacga
    46021 gagacag
//
LOCUS       DAZPBM010000014        44183 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPBM010000014 DAZPBM010000000
VERSION     DAZPBM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.014"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHUZQ_000697"
     CDS             complement(<1..559)
                     /locus_tag="ACHUZQ_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR1024482.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUZQ_000698"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHUZQ_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1024483.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUZQ_000699"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHUZQ_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1024484.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUZQ_000700"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHUZQ_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1024485.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHUZQ_000701"
     CDS             complement(4001..6322)
                     /locus_tag="ACHUZQ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1024486.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUZQ_000702"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHUZQ_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1024487.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUZQ_000703"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHUZQ_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1024488.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUZQ_000704"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHUZQ_000704"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1024489.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUZQ_000705"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHUZQ_000705"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1024490.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHUZQ_000706"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHUZQ_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUZQ_000707"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHUZQ_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1024491.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUZQ_000708"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHUZQ_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1024492.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUZQ_000709"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHUZQ_000709"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1024493.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUZQ_000710"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHUZQ_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1024494.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUZQ_000711"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHUZQ_000711"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1024495.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUZQ_000712"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHUZQ_000712"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1024496.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUZQ_000713"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHUZQ_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1024497.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUZQ_000714"
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHUZQ_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1024498.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUZQ_000715"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHUZQ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1024499.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUZQ_000716"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHUZQ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR1024500.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUZQ_000717"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHUZQ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1024501.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUZQ_000718"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHUZQ_000718"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1024502.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUZQ_000719"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHUZQ_000719"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1024503.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHUZQ_000720"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHUZQ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1024504.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHUZQ_000721"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHUZQ_000721"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1024505.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUZQ_000722"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHUZQ_000722"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1024506.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHUZQ_000723"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHUZQ_000723"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1024507.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUZQ_000724"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHUZQ_000724"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1024508.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUZQ_000725"
     CDS             31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHUZQ_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1024509.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="ACHUZQ_000726"
     CDS             32202..32564
                     /locus_tag="ACHUZQ_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1024510.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="ACHUZQ_000727"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="ACHUZQ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="ACHUZQ_000728"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="ACHUZQ_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="ACHUZQ_000729"
     CDS             35034..35912
                     /locus_tag="ACHUZQ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1024511.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="ACHUZQ_000730"
     CDS             35923..36276
                     /locus_tag="ACHUZQ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1024512.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="ACHUZQ_000731"
     CDS             36288..37592
                     /locus_tag="ACHUZQ_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1024513.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="ACHUZQ_000732"
     CDS             37604..38689
                     /locus_tag="ACHUZQ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1024514.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUZQ_000733"
                     /gene_synonym="yhfR"
     CDS             complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHUZQ_000733"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1024515.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUZQ_000734"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHUZQ_000734"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1024516.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUZQ_000735"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHUZQ_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUZQ_000736"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHUZQ_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1024517.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHUZQ_000737"
     CDS             complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHUZQ_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR1024518.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(44010..44177)
                     /locus_tag="ACHUZQ_000738"
     CDS             complement(44010..44177)
                     /locus_tag="ACHUZQ_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1024519.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT        10306 a        11209 c        12099 g        10569 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctagttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac aattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatag ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctcc tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaggc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggt gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaacccg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt cccatagcga tacctttttc ttgtccaata
    43741 cgttgtaggg gagagtttgc agtcatttaa aaggtaacat gacaatgcat gatgaatata
    43801 acaacatatg atgttatgta atgtggagga agatcacaaa atttcgcaca ggatcgcgct
    43861 gtggctaatg gatgtagtta tcaaattgaa tttaaagtga aaatattttt acgggcgggg
    43921 gcaagaagga catagaaaca aatacgccct cggaaaatcc agagggcgtc gggcaattaa
    43981 accggtgtta gccgatttct gtcagagact tactgtgcag taggaccgca accgccaatg
    44041 attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt cttatcacgg
    44101 ttttcaatat gaccagtaca tgcagttaac gtagccagta ctgcacctac cagtgcaact
    44161 ttaataaatt tgttcattta cat
//
LOCUS       DAZPBM010000015        43821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPBM010000015 DAZPBM010000000
VERSION     DAZPBM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.015"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            236..1138
                     /locus_tag="ACHUZQ_000739"
     CDS             236..1138
                     /locus_tag="ACHUZQ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1024520.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1285..2505
                     /locus_tag="ACHUZQ_000740"
     CDS             1285..2505
                     /locus_tag="ACHUZQ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1024521.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2478..3107
                     /locus_tag="ACHUZQ_000741"
     CDS             2478..3107
                     /locus_tag="ACHUZQ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1024522.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(3108..4268)
                     /gene="ybdL"
                     /locus_tag="ACHUZQ_000742"
     CDS             complement(3108..4268)
                     /gene="ybdL"
                     /locus_tag="ACHUZQ_000742"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1024523.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4377..5465
                     /gene="hcxA"
                     /locus_tag="ACHUZQ_000743"
     CDS             4377..5465
                     /gene="hcxA"
                     /locus_tag="ACHUZQ_000743"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1024524.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5475..5672)
                     /locus_tag="ACHUZQ_000744"
     CDS             complement(5475..5672)
                     /locus_tag="ACHUZQ_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1024525.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5854..7959)
                     /gene="cstA"
                     /locus_tag="ACHUZQ_000745"
     CDS             complement(5854..7959)
                     /gene="cstA"
                     /locus_tag="ACHUZQ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1024526.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(8140..8553)
                     /gene="entH"
                     /locus_tag="ACHUZQ_000746"
     CDS             complement(8140..8553)
                     /gene="entH"
                     /locus_tag="ACHUZQ_000746"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1024527.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8556..9302)
                     /gene="entA"
                     /locus_tag="ACHUZQ_000747"
     CDS             complement(8556..9302)
                     /gene="entA"
                     /locus_tag="ACHUZQ_000747"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1024528.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9302..10159)
                     /gene="entB"
                     /locus_tag="ACHUZQ_000748"
     CDS             complement(9302..10159)
                     /gene="entB"
                     /locus_tag="ACHUZQ_000748"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1024529.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10173..11783)
                     /gene="entE"
                     /locus_tag="ACHUZQ_000749"
     CDS             complement(10173..11783)
                     /gene="entE"
                     /locus_tag="ACHUZQ_000749"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1024530.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11793..12968)
                     /gene="entC"
                     /locus_tag="ACHUZQ_000750"
     CDS             complement(11793..12968)
                     /gene="entC"
                     /locus_tag="ACHUZQ_000750"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR1024531.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13343..14308
                     /gene="fepB"
                     /locus_tag="ACHUZQ_000751"
     CDS             13343..14308
                     /gene="fepB"
                     /locus_tag="ACHUZQ_000751"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1024532.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSVLALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(14389..15639)
                     /gene="entS"
                     /locus_tag="ACHUZQ_000752"
     CDS             complement(14389..15639)
                     /gene="entS"
                     /locus_tag="ACHUZQ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1024533.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15750..16754
                     /gene="fepD"
                     /locus_tag="ACHUZQ_000753"
     CDS             15750..16754
                     /gene="fepD"
                     /locus_tag="ACHUZQ_000753"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1024534.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16751..17743
                     /gene="fepG"
                     /locus_tag="ACHUZQ_000754"
     CDS             16751..17743
                     /gene="fepG"
                     /locus_tag="ACHUZQ_000754"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1024535.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17740..18555
                     /gene="fepC"
                     /locus_tag="ACHUZQ_000755"
     CDS             17740..18555
                     /gene="fepC"
                     /locus_tag="ACHUZQ_000755"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1024536.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18552..19685)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUZQ_000756"
     CDS             complement(18552..19685)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUZQ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1024537.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19901..23782)
                     /gene="entF"
                     /locus_tag="ACHUZQ_000757"
     CDS             complement(19901..23782)
                     /gene="entF"
                     /locus_tag="ACHUZQ_000757"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1024538.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(23779..23997)
                     /gene="ybdZ"
                     /locus_tag="ACHUZQ_000758"
     CDS             complement(23779..23997)
                     /gene="ybdZ"
                     /locus_tag="ACHUZQ_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1024539.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(24000..25202)
                     /gene="fes"
                     /locus_tag="ACHUZQ_000759"
     CDS             complement(24000..25202)
                     /gene="fes"
                     /locus_tag="ACHUZQ_000759"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1024540.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25445..27685
                     /gene="fepA"
                     /locus_tag="ACHUZQ_000760"
     CDS             25445..27685
                     /gene="fepA"
                     /locus_tag="ACHUZQ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1024541.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27860..28480
                     /gene="entD"
                     /locus_tag="ACHUZQ_000761"
     CDS             27860..28480
                     /gene="entD"
                     /locus_tag="ACHUZQ_000761"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1024542.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28602..28754)
                     /gene="hokE"
                     /locus_tag="ACHUZQ_000762"
     CDS             complement(28602..28754)
                     /gene="hokE"
                     /locus_tag="ACHUZQ_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1024543.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(29105..29257)
                     /gene="hokE"
                     /locus_tag="ACHUZQ_000763"
     CDS             complement(29105..29257)
                     /gene="hokE"
                     /locus_tag="ACHUZQ_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1024544.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            29226..29494
                     /locus_tag="ACHUZQ_000764"
                     /pseudo
     CDS             29226..29494
                     /locus_tag="ACHUZQ_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29697..30815
                     /locus_tag="ACHUZQ_000765"
     CDS             29697..30815
                     /locus_tag="ACHUZQ_000765"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1024545.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            30881..31129
                     /locus_tag="ACHUZQ_000766"
                     /pseudo
     CDS             30881..31129
                     /locus_tag="ACHUZQ_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            31194..31562
                     /locus_tag="ACHUZQ_000767"
     CDS             31194..31562
                     /locus_tag="ACHUZQ_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1024546.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31656..32309
                     /gene="nfsB"
                     /locus_tag="ACHUZQ_000768"
     CDS             31656..32309
                     /gene="nfsB"
                     /locus_tag="ACHUZQ_000768"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1024547.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            32417..33664
                     /gene="ybdG"
                     /locus_tag="ACHUZQ_000769"
     CDS             32417..33664
                     /gene="ybdG"
                     /locus_tag="ACHUZQ_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1024548.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33732..35108)
                     /gene="pheP"
                     /locus_tag="ACHUZQ_000770"
     CDS             complement(33732..35108)
                     /gene="pheP"
                     /locus_tag="ACHUZQ_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1024549.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(35210..38353)
                     /gene="cusA"
                     /locus_tag="ACHUZQ_000771"
     CDS             complement(35210..38353)
                     /gene="cusA"
                     /locus_tag="ACHUZQ_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1024550.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(38365..39588)
                     /gene="cusB"
                     /locus_tag="ACHUZQ_000772"
     CDS             complement(38365..39588)
                     /gene="cusB"
                     /locus_tag="ACHUZQ_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR1024551.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(39604..39936)
                     /gene="cusF"
                     /locus_tag="ACHUZQ_000773"
     CDS             complement(39604..39936)
                     /gene="cusF"
                     /locus_tag="ACHUZQ_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1024552.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39960..41342)
                     /gene="cusC"
                     /locus_tag="ACHUZQ_000774"
     CDS             complement(39960..41342)
                     /gene="cusC"
                     /locus_tag="ACHUZQ_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1024553.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            41499..42182
                     /gene="cusR"
                     /locus_tag="ACHUZQ_000775"
     CDS             41499..42182
                     /gene="cusR"
                     /locus_tag="ACHUZQ_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1024554.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            42172..43620
                     /gene="cusS"
                     /locus_tag="ACHUZQ_000776"
     CDS             42172..43620
                     /gene="cusS"
                     /locus_tag="ACHUZQ_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1024555.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10636 a        12022 c        11057 g        10106 t
ORIGIN      
        1 catcacccat tatgggaaat aaataaaagg taattcatgg atggttgaac tatatccctg
       61 cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg ctgtgctatt
      121 ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat acatacgcaa
      181 aaaacattga ttaagtgaat ataccatgga agaaaaatat aaaccggagt agtgtatggc
      241 caatctctac gacttgaaaa aattcgactt aaatcttctt gtcatatttg agtgtattta
      301 ccagcattta agcattagca aagctgctga atcactatat atcaccccat cagccgtgag
      361 ccagtcgtta caacgcttac gtgcgcaatt taatgaccct gtattcattc gctcaggaaa
      421 aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaaacctcaa
      481 aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat
      541 ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca
      601 agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga
      661 actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc
      721 cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta ataagcatcc
      781 gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat
      841 atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt ttatgaatcg
      901 caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg cagttacaga
      961 tttactggga atagtcccct atgaacttta taactcccac cgtgactttc ttaatttaaa
     1021 agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct ataataaatc
     1081 ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga gtttttgata
     1141 tataactaca gacagctact ataactttca tctatttatt cacagcgcga atgaaaaata
     1201 aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac tgttcaccct
     1261 gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc gctcaatatt
     1321 ctggaagctg cacgagagcg tattacctgg acactcaaca ccctaccccg cgtttgtgtc
     1381 tcattttctg gcggcaaaaa ctctggttta atgctgcatc tgacagccga acttgcccga
     1441 caaatgggca aaaaaatctg cgttttgttt atcgactggg aggcgcaatt ctcttgcacc
     1501 attaactatg ttcagtccct gcgcgagttg tacaccgatg tcatcgaaga gttttactgg
     1561 gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat accaacccga atggcagtgc
     1621 tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag atgcgataac cgatcctgac
     1681 tttttctgct tttaccagcc aggcatgacc ttcgaacaat ttgtacgtga gtttgccgaa
     1741 tggttttcac aaaaacgtcc ggtggcgatg atgatcggca tccgtgcgga tgagtcctac
     1801 aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgacga taaaccatgg
     1861 actacggccg caccaggcgg tcatagctgg tacatttacc ccatttacga ctggaaagtg
     1921 gctgatatct ggacctggta tgcaaatcat caaaatctct gcaacccact gtataaccta
     1981 atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt tggcccggag
     2041 caacgacaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc tgctatgtgc
     2101 gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa tcatttctat
     2161 gggcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc attattgctg
     2221 ctcaatagca tgccggaaaa aacagctgag cattaccgca ataaaatcgc catttatttg
     2281 cactggtatc agaaaaaagg catcgaggtt ccacaaaccc agcaagggga cattggcgcg
     2341 aaagatatcc cctcctggcg gcggatatgc aaagttttac tcaataacga ttactggtgt
     2401 cgggcattat catttagtcc tacgaaagcg aagaactatc agcgttataa cgaacggata
     2461 aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg attaacgcag gatttaacgc
     2521 aatttctcgc cagcttgccg gaagatgatc gcatcaaagc gattaatgaa atccgcatgg
     2581 cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg tgggttaaaa
     2641 acagccaact tatgcctaat gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac
     2701 tgctgcaaaa atccattgaa atagatggtt ttacccaacc cattgtggtc acacatacgg
     2761 ataaaaatgc catggaaatc gtggatggtt ttcaccgcca tgaaattggt aaaggctcat
     2821 catcattaaa attacgctta aaaggttatt tgccggtgat ctgtctggaa ggtacgcgta
     2881 atcaacgcat cgccgcgact attcgtcata atcgcgctcg tggacgccat caaatcaccg
     2941 ccatgtcgga aatcgtccgt gaacttagcc agttgggatg ggacgataat aaaattggca
     3001 aagagctggg aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac ggcctgcaag
     3061 agctgtttgc cgaccgtcaa tattcccgcg cctggacggt taaataacta aagctggcgc
     3121 aggcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag acgaatcagt
     3181 ttatgtggga agggatcggc gcaaaacacc gacagcggaa tcgccgctac gccgtgctcc
     3241 tgcgtcagcc actggcaaaa ctcaacatca tccagggtag aaaccgcgct gtaatccacc
     3301 agcaaaaagt atgtaccttc acacggtaaa atctccagcc ggctttcatt taaggcattt
     3361 accagaatat cgcgcttctg gcgataaaag tccggtaacg caagataatg ctcaggttct
     3421 gcacgtagca tatcagcaag cgccagctgt gccggggtat tcaccgaaaa ggtcagatac
     3481 tgatgtacct tgcgaatttc ggcgctgatg ggcgctggcg caacacaata acccactttc
     3541 cagccggtca tatgataggt cttgccaaat gaagaaaccg ccactgcccg ctcacgcagc
     3601 tgcgaatgcg ccagcacact ggcatggccc tgttgtgaaa agttgatgtg ctcgtagact
     3661 tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc ggcgaaatca
     3721 gcctgctgcc agacagttgc actggagtta tgcggagtgt tgaggatcac cagtctggtg
     3781 cgctcgctta ataatgcggc aaattcctgc cagtcaacgc gaaaatgcgg tggttgcagt
     3841 gccatacgct tcactattcc cccagaaagc gcgatggcgg gggcgtaact gtcatagctg
     3901 ggatcaaaac aaatcacttc atcgccattg cgcaccagtg cggtaatcgc cgcgtataac
     3961 gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg atagccatac
     4021 aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt catgggcgcg
     4081 tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata gcgcggacca
     4141 tcaaaatcag gaaagccttg cgacaggtta atcgcctggt gttgctgcgc cagcgcgctc
     4201 atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat cagagggtta
     4261 tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa cacgatgcta
     4321 gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa cacaacatgc
     4381 ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat ccaggaagtt
     4441 tcaatcacct gaacgatttt ttcactgatg aacaactttc tcgcgcggtg tggatctacg
     4501 gcaaacgcgc cattgctgcg gcgcaaacca aacttccgcc agcgtttgga cttccagggg
     4561 caaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa ctggcggctg
     4621 agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg cggcgcactg ctcgacaccg
     4681 cgaaagccct cgcccgccgt ctcggtctgc catttgttgc cgttccgacg atcgccgcta
     4741 cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag gcgctgcatt
     4801 atgagatttt cgacgacgcc aattttatgg tgctggtgga accagagatt atcctcaacg
     4861 cgccgcaaca atatctgctg gcggggatcg gtgacacgct ggcgaaatgg tatgaagcgg
     4921 tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcgactgggg atcaataatg
     4981 cgcaggccat tcgcgacgta ttgttaaaca gtagcgaaca ggcgctgagc gatcagcaaa
     5041 atcaacagtt aacgcaatca ttttgcgatg tggtggatgc tattattgct ggtggtggga
     5101 tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggctcatgcc gtgcataacg
     5161 gtctgactgt cctgccgcaa accgagaaat ttctccacgg caccaaagtc gcttatggca
     5221 ttctggtaca aagcgccttg ctaggtcagg atgatgtgct ggcgcaatta accggggcgt
     5281 atcagcgttt tcatctgccg accacgctgg cggagctgga aatggatatc aataatcatg
     5341 cggagatcga caaagtgatt acccacaccc tgcgtccggt ggaatacatt cattacctgc
     5401 cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaaagtggaa tcgtttaaag
     5461 cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc caccgtagcg cgcgtcctgc
     5521 cgctcacgga aaaactcttc gtaggtcatc ggcgtttgat cgggatggtt aacccgcata
     5581 tgttcgacat agttgtcgta atcaggcata ccaatcatca gcttcgccgc ctgacctaaa
     5641 tattttccgg cttttgccag tgaatcaaac atcttggcct ccgctaacca tgttgctgaa
     5701 agagtgccgg atgcggcgtg aacgccttat ccggcctaca aatgctatca acgcgctcgg
     5761 tcagtcccct cgccccatcg gggagagggt tagggtgagg aggaaccctc ggcactatgc
     5821 caacattcgc caacatcccc cctcactctg actttagtgt gcgccttttg cctgcgccac
     5881 gatctcctcg acattttccg gcattggctc atacggcgtt tctttcgccg ttggcttcgg
     5941 atctttcaat gccgccagtg ccgacttaat cgagaacagc gccagaacca ccacgaccac
     6001 cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt gcgccagctg
     6061 tgactcagta tactgcgacg gaatattgcc gctgtcgatc attgcctgga acttattagc
     6121 aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc ctgcggtcag
     6181 ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac gttgacgttt
     6241 catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca tctggttggc
     6301 aataccaaac agcggccaca gagtgttaat gccgcccagc ggatcgacca cgccctgatg
     6361 gaggaagtag ccccacgcca gtacgcacag cgctgttgcc agcaggttag caggcagtga
     6421 atcggtccgc ttcaggccag gagacaccac gcccagcaga tcctgcaaca taaagcgcgc
     6481 agcacgcgta cccgcatcta ccgccgtcag aataaacaga gcttcaaaca gaatggcgaa
     6541 gtgataccag aacgccacat ccatcatacc acccagcgca ccgtgcagga tgtaagccat
     6601 ccccaccgcc agcgtcggcg caccgcccgc acgggaaatg atcgactgtt cacccacttc
     6661 gctggcaatc tggttgagcg tatccggagt aatcgcaaag ccccagctac tcaccacctg
     6721 cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca tcgggctgtt
     6781 catggcgaag tacacgcccg gatcgatgat acaggcggaa accagtgcca taatcgccac
     6841 gaaggattcc attaacattc caccgtagcc gataaagcac gcctgccctt cgttcgccag
     6901 catcttcggc gtggtgccgg aagagatcag cgcatggaag cctgacaccg cgccacaggc
     6961 gatggtgata aacaggaacg ggaacagatt accggtccat accgggccag tgccatcgac
     7021 aaatttggtc agcgcaggca tggtcagcgt cggacgcata atcaaaatgc ctaccgccag
     7081 accaacgata gtcccgattt tcaggaaggt agagaggtag tcacgcgggg ccagcagtaa
     7141 ccacaccggc agcaccgccg ccacaaaacc gtagcccacc agcatccagg tcagctgcac
     7201 gccggtaaag tcaaagtacg gtgcccaggt cgggctttct gccacccagc cgccagagat
     7261 aatggcaaaa ataaggaaca ccaggccaat cactgacact tcgccaatac gccccggacg
     7321 cagatagcgc aggtagattc ccataaaaat cgccagcgga atggtgaacg caacggtata
     7381 cgttccccac gggctatgag tcagggcttt caccacgatc atcgccagta ctgcaaggat
     7441 aatgaccatg atcataaagc aggctaccag cgcaatcacc ccggcagttg gccccatctc
     7501 ttctttgacc agctcaccca gcgagcgacc gtcacggcgc gtagaaacaa acagcaccat
     7561 gaaatcctgc accgcaccgg cgagaaccac cccggcgagc agccagatca tccccggcag
     7621 gtagcccatt tgcgctgcca gcaccggccc caccagcggc cctgctccgg caatggccgc
     7681 aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc cgtcgttatg
     7741 gcgcaccgct ggcgtcatac gcgtcgggtc aaccgccagc acatttttgg cgatatacag
     7801 cccataaaaa cggtaagcga tcagatagat acagaccgac gccaccacaa tccacagcgc
     7861 gttgatctgt tccccacgat ttaaagcaat gtatcccaga gcaaatgctc ccattacaga
     7921 gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc gtgtgcgtgt
     7981 ccatagagat gttacatttt gtttctataa catctttgta cgatttaaca acacggtaac
     8041 catgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga tcactccgtt
     8101 aaccgagttt tcctgcaatc tcatttaatt ctgtcgggct catcccaaaa ctgccgtacc
     8161 cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgatgattt cccagctctg
     8221 attttgccgc ccaagatgca gcggctggca gacgccgcgt accttgcctt cagacaccgg
     8281 gcgatgatgg gtggcgttaa gttccgtacc caccacacac tgcccgtcac gggtcatcat
     8341 aaatccggcc atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc catgcagcag
     8401 gccaaacggc tgatgagtac gggtatcaac cggcatttcg gcttccagca catcatctcc
     8461 cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg tggcgttcag
     8521 ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt tgagccgcca
     8581 tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag gaacaaaatc
     8641 gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag tttaaactgc
     8701 tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag cgtgcgttgc
     8761 atatcggtgt cggtggagcc aggcgaaacc acattacagc gcacgccgct aaccgccagt
     8821 tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc ataagcactc
     8881 ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc cccgccccgc
     8941 tgacggcgaa actggttcat ggtttgctga aacaggttaa acgcaccgcc gacgttaacc
     9001 gcaaaagtct gctgccagtc ctctttactg agctgatcgg tcgcgcccat gcgcaaaatt
     9061 cccgccgcat tgaccagcac gtccagtcgc tccgtttcag ctaacagtcg ctgacatacc
     9121 tgcgcgacct gcgcagcatc ggcaacatcc atcacttcgg tcgcaaaggg atattgctcc
     9181 tgagcgaacg cttgatcaaa accggtaact ttcgctcccg cttcaacaaa cgccagcgcc
     9241 gtggcgtagc cgataccttt acctgcgccg gttacccaga catttttacc gctgaaatcc
     9301 attatttcac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg tttttcgcca
     9361 gcatgacaaa gtcgatatcg ccatgcactt tgcgccagcg cgccgccagc gccatcatgc
     9421 gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca tcggactcgt
     9481 ccagcaacgg caggataacc tcacgcagcg ccgctttcct ggcggggaca ggtgctggca
     9541 gtaatccttc agtcatcacc acccggccag accgcccggc cacatatttc agcgacatca
     9601 aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt ttaatatcgc
     9661 gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc ccggtaataa
     9721 tcagctggtt acgtccactc tctttgagca tttgctccag cggagaacga tgaaacgcgc
     9781 tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcaggcga tccaccacct
     9841 tttgctgttc cggcgaacgg gtcaggcccg gcccccacat atcattcaac agcgcccgat
     9901 cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg tgctgtttgc
     9961 agtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg cagttctcgc
    10021 cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca cgttgcggtt
    10081 caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt gcgtaagcct
    10141 gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga cgccagccac
    10201 tgacgtaatt gttttttatc gactttcccg accgccgtca gcggaagtga atccacacac
    10261 tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa acgacgcacc
    10321 tgcaccgcgc gcagcggctc ttttaccacc agataagcac agcttttttc gcccatcagc
    10381 tcatcttcca tgctcaccag tgcggcatag atcaccgccg gatggcgcag cagcaggttt
    10441 tcgatctctt cggcagcgat cttctcgccg ccacggttga tctgatcttt ctcgcgcccc
    10501 tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca gtaaaaaccg
    10561 ttggcatcaa aggcactggc attgtgctgc ggacttttgt aataaccgcg gaaggtgtac
    10621 ggcccgcgcg tcatcaggcg tccaacttcc ccttgcggca gtggatttcc ttcagcatca
    10681 gcaacccata cttcatcgtc cggacacatt gggtaaccct gggtatggat aattttctcc
    10741 gcgctatcat caagtcgggt gtagttcacc agcccttccg ccatgccaaa cacctgctgc
    10801 aactggcagc caatctcagc ggaaatacgc gccgcaaggg tggcagaaag acgtgcgccg
    10861 ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc agccagcgcc
    10921 tgcaaccaca ggctgactgc cggcggcacc agcgcggtaa cgttaacctg atgttttgca
    10981 atcaacggga agcaaagcgt ggcgctggga tcggccgcca gaacaaccgt tcctccggca
    11041 agaaagacgc ccagcgatcc tggcgaactc atagcgtagt tatgagccgc cgggatcgcg
    11101 cacaggtagc gtgtctgttg tgtgaactga caaatctcga cgctacgacg cacgctgtag
    11161 tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc gccggaaagc
    11221 tggaaatagg ccacttcatc agcaggtgat ggcgtagcag taaaaccgtc tgccggatgg
    11281 ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttga gcagttgcac cacgcgaatg
    11341 gaggaatgtt ctgtgacgaa agtattgagg aaatcatccc cgctaaacag cgcatgttgg
    11401 cgatcggcaa tcagtaacgc cggttcaatc tggctggcat aggcgttcag ttcactacgc
    11461 tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagtgc gaaaaaggta
    11521 atgtaaagtt cagcgacgtt acccagttgt accagcgcgg tttcaccggg tttaatgccc
    11581 tgacggcgta aactacacgc gaggttatcc gccgcctgat tcagctcccg gtaactcaac
    11641 tgtcgctcgc cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt cagaatgtcg
    11701 gtcagcggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa ctcttccggc
    11761 cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac gttcaacatg
    11821 gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc aggcacaatc
    11881 cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcgcagcg gatggtcacc
    11941 acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa cagttcgcgg
    12001 tcaaacggct ccagttcagc aataacctgg gtcgcggcct gatgcgggaa accgctcagc
    12061 gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga attcgcttta
    12121 ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg taatcaattg tggagaggag
    12181 ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg agtcaccagt
    12241 tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacccgcttc gcgatcgagc
    12301 acttcatccg gctgacgacg cgcggaaccg gctaacggaa tggagctaaa acgctcgccg
    12361 tctttacgta gcagcagttc cgggctggcc cccagcagga cgccaccatc agccagcggg
    12421 acatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa tacgccacta
    12481 tcaatggcgg cgtcagtggt gatatcaatc aatcgtgaca acaccacttt gtcgacctgc
    12541 ggcgtggcgg taagtgcggc ggcgcgggca accatctgtt caaacgtggt ttgttcagga
    12601 attgcctggc gttccaccac gttcagcgac tggctgcggg taaaacagtg ggctgaggtc
    12661 tgtttttcct gacgggagaa cgactgccag gattcgggaa tatacagcga cgaaggctga
    12721 cgtggatcga agggaatcgc cccgaccatc accggatttt tgatgccctg cgctttggca
    12781 tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc cccgttcaca
    12841 gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta cggcgacata
    12901 aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc agccagtgac
    12961 gtatccatat catcctccac aaaatgataa aggctttaat aatgattatc atttatattt
    13021 tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa cgcaatctgc
    13081 aactcggggt tgcatctggt tgtggttaca atgaaaatga gaagcattat tgatgaattc
    13141 gcataagcgc aatgtgatgt cctgcgccgt tttgccccct ctcccttcca gggtgagggc
    13201 tggggtgagg gttaatgttc gcaccagtgc tggctgttcc cctcacccta accctctccc
    13261 caaaggggcg aggggacgga ttgtgcgctt tgtcgaattt gtcattacgc ccttaacctt
    13321 attaataaca ggaagctgac ttgtgagact cgccccgctc taccgcaacg cccttctatt
    13381 aacaggactt ttgctttcag gaatagccgt agttcaggcc gccgactggc cgcgtcagat
    13441 tactgacagc cggggcactc ataccctgga aagccagccg cagcgtattg tttccaccag
    13501 cgtcaccctg accggatcac tgctggcgat tgatgctccg gtgatcgcca gcggcgcgac
    13561 cacgccgaat aaccgcgtcg cagatgacca gggcttttta cgccagtgga gcaaggtggc
    13621 gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcgccgaag ccgttgccgc
    13681 gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggtgc tggcgctgta
    13741 tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca aaagctggca
    13801 gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg cagagcggat
    13861 tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac cgccgcagcc
    13921 ggtcactgcc attgtctata ccgccgctgc acacagtgcc aatctctgga cgccggaatc
    13981 agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttgc ccgcagggtt
    14041 aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg gggaaaatct
    14101 ggctgctggg ttaaacggtg aatcgctatt cctgttcgcc ggtgatcaga aagacgccga
    14161 tgcgatttat gccaatccac tgctcgcgca cctgcctgca gtacaaaaca agcaggttta
    14221 tacgctggga accgagacgt tccgcctgga ttactacagc gccatgcaag tgctggacag
    14281 acttaattca ctgttaaaaa aagattgata aaacatacaa ttagagcact aataatcccc
    14341 tcgccccttt ggggagaggg ttagggtgag gggaaaatgc acctttagct aactgtcaga
    14401 cgctgtcacc tgcggcggcg tctggcgaaa acgtcgcaac tccaccagca ccagcaataa
    14461 caacacgccg ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat
    14521 catcgcgccc aaaccaccca acagcgccgc gcctatggca tcgcccgtca cgttctgcgc
    14581 cgtccacaaa ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt
    14641 atattgcagc aacgaactaa ccgcgctcag ccagccgaac agcgccagac aaaccacgcc
    14701 taaaatccac atcggcatca ggccaaacag accaatggcg aggaacgatc ccagcgtgga
    14761 gagcagcatc aataaccctg gtcgcgcact atgtgccagc ttcccgctgg ttaacgcacc
    14821 aatagccgca ccgagcggga tcgccgcata gagaaaacca atctgtgccg ccgacatctg
    14881 ccagttgtca gccagcgccg gatacagtac ccgcaccgcg ctcgccatcg tcaataaacc
    14941 acccagcaac gcaatccctc ctaccagcgg gctggcgagc agaaaacgaa atcctgccag
    15001 taatgatttc aacggatgct cgcgcggctg cggtggcggt ggcaacgccg gaaggcttaa
    15061 caacggtagc aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggctacgcc
    15121 gccggtcgcc agcaataaac cgccaatcat gggcgaaatc accgacccca gacgcacggt
    15181 caacatggtg atcgccccgg cctgcattaa gttttcacgc cctaccagtg caggtgttgc
    15241 cgccagtagc gccgtaacac caagtgatgc gaaaaaacca tcccataaac caagtaaata
    15301 gattgccagc aatgacggct ccggcagcag cgcgttcagg cacagtccaa tgaagccaat
    15361 gccacaggtg ccgcgcgcca gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac
    15421 accgccaacc atcaggccaa caaacatcgc gccgccggtc agcgtcaccg aaagccccac
    15481 ctgccaggta gaatgcgtca tcatctggat ctgcaccggc accgcgacgc cgagcaaacc
    15541 cagagacaca attgagataa aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt
    15601 caacaggctg aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat
    15661 attttaaata cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga
    15721 taattaattt cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca
    15781 ttgccgtgcc cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg
    15841 gggcaaaatc actccccgcg tcagtagtgc tggaggcctt ctcaggcacc tgccagagcg
    15901 ccgactgcac catcgtgctc gacgcgcgac tgccgcgcac ccttgccggt ttacttgcag
    15961 gcggtgcgct tgggcttgct ggggcgttaa tgcaaaccct cacccgaaat ccacttgccg
    16021 accccggctt gcttggcgtg aacgccggag ccagctttgc cattgtgctg ggtgcggcgc
    16081 tgtttggtta ctcatccgcg caggaacaac tggcgatggc cttcgccggg gcgctggtgg
    16141 cctcattgat tgttgccttt atcggcagtc agggcggcgg gcagttaagt ccggtgcgtt
    16201 taaccctggc gggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc
    16261 tgcttaatcc tgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc
    16321 gcaatctaca taccttaaaa gtggtgctga tcccggttct gatcgccgga gcaactgcgc
    16381 tattactgag tcgcgcgctg aacagtttga gcctcggcag tgacaccgcg acggcgctgg
    16441 gcagtcgcgt ggcgcgcaca cagttgattg gtctgctggc gattaccgtg ctttgtggta
    16501 gtgcgacggc ggtagttggc ccgattgcct ttatcggcct gatgatgccg cacatggcgc
    16561 gctggctggt aggtgccgat catcgctggt cgctgcccgt cacgctactt gctacccctg
    16621 ccctgctgct gtttgccgat atcatcgggc gggtgattgt gcccggcgaa ctgcgcgttt
    16681 ctgtggtcag tgcgtttatt ggtgcaccgg tgctgatctt cctcgtgcga cgtaaaacgc
    16741 gaggtggtgc atgatttacg tctctcgccg attactcatc acctgtttgc tgctgatttc
    16801 cgcctgtgtg gttgcaggta tctggggatt acgcagcggt gccgtcacgc tggaaatctc
    16861 gcaggtattc gccgcgttga tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga
    16921 atggcgttta ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg
    16981 cgcgattttt cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa
    17041 caccggggcg tggagcggcg tgctggtggc gatggtgctg tttggtcagg acctgacggc
    17101 tatcgcgctg gcagcaatgg tgggcggcat tgtcacttct ctgttggtct ggctgctcgc
    17161 ctggcgtaac ggtatcgaca cctttcggtt gattattatc ggcatcggcg ttcgcgccat
    17221 gctggtggcc tttaatactt ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc
    17281 gggtttgtgg aatgccggat cgctcaatgg cctgacgtgg gcaaaaactt cgccttccgc
    17341 acccatcatc atattgatgc tcattgccgc cgccttgctg gtgcggcgaa tgcgcttgct
    17401 ggagatgggc gatgataccg cctgcgcgct gggcgtcagc gtcgagcgtt cgcgtctgtt
    17461 aatgatgctg gttgcagtgg tgcttaccgc tgcggcaacg gcgcttgccg ggccgatttc
    17521 ctttattgct ttagtcgcgc cgcacattgc ccgacgcatt agcggcaccg ctcgctgggg
    17581 gctgacccag gcggcgctgt gcggagcgct gttactgctg gcggccgatc tctgcgccca
    17641 acaactgttt atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggcggtat
    17701 ttaccttatc gtcttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt
    17761 ttgcgcggcg aacagttaac cctgggatat ggcaaatata ccgttgcgga aaatctgact
    17821 gtagaaattc ctgatggtca cttcacggca attatcggac caaatggctg tggtaaatcc
    17881 acgttactgc gtaccttaag ccgcctgatg acgcctgctc acgggcatgt ctggctggat
    17941 ggcgagcaca ttcaacatta cgccagtaaa gaagttgcac gccggattgg tctgttggcg
    18001 caaaatgcta ccacgccggg cgatatcacc gtgcaggagc tggtggcgcg tggacgttat
    18061 ccgcatcaac cgctgtttac ccgctggcgc aaagaggatg aagaagcggt aacgaaagcg
    18121 atgcaggcca cgagaataac tcatctggca gatcaaagcg tggataccct ttctggcgga
    18181 caacgccagc gagcgtggat cgcgatggtg ctagcccagg aaacggcaat tatgctgctc
    18241 gacgaaccca cgacctggct ggatatcagt catcagattg atctactgga actgttaagc
    18301 gaactgaacc gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc
    18361 tgtcgttacg ccagccattt gattgcattg cgggaaggga aaattgttgc tcagggagcg
    18421 ccgaaggaga ttgtcactgc tgaactgatt gagcgcattt atggtctgcg atgcatgatc
    18481 attgacgatc cggtagccgg aacgccgctt gtggtgccgc tcggacgaac ggcaccttca
    18541 accgcaaata gttaaactaa gtggtctgcc atcatggcat cctgttttct ggatgccatc
    18601 gcatagcgca ataacacgct accacaagcc accatcccgc cgatcagcgc ggaaaggatc
    18661 acaataatcg ccttacccgg accgtctttt ttcactggca atgacgggct taactgatat
    18721 ttaaacggcg taaaattcac atcgttgaca tttgcttttg ttaattgctc gacaagatac
    18781 tgccgattac gtaattcacc gttcagttcc gcaacgtcag taaccgcttt ttcaatttcc
    18841 agtttgcgtt caataccgtc tgcgccgaga gaaatagaga aatcggggtc atctttaacg
    18901 gcctgaccat tactgtatac gggcttttta attcctgccg cgttggcaat gtcgagtgaa
    18961 taattgaggc gctgaatgtt tgcatcaagt tgatttttcg ttttaatgcg atcctgagcc
    19021 agtttttctt tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac
    19081 tctttcacca ccaacgcaga gatataatcg atatacccgc tcaaaacggt ctgcgcctct
    19141 ttactggttg gcgcggtaaa acttagcgtc caggaggtat acagtgacgg ttcatctttt
    19201 ttcttactgg cattgtcatc aaccgctttc attttttcgc tcaatgcaac aatagcgcga
    19261 tgcaaatcca gttcatcgat tttcgcctct tttaattggt ccatcacata aggtgatgaa
    19321 cgcaggtact cttccagcaa gctaacagac tgaaacttct tgataaacag gttaaatgca
    19381 ccgccacggt cgatattaac atcgagatca agaacgcgga gtttggtaaa ggttttctcc
    19441 aggtcctgcc actgaatggc ttcggcggga gtaataacgg ctgagctggt ccatttttgc
    19501 ggcaggatga aagagatcag taaacctgcg caggcaaatg caaaaacgac cgccatgacc
    19561 gtttttttgg cccgccataa aacttcgatt agattaagta aatcaatttc attattactg
    19621 ggcgacgcca gaggatactc agaaaaatga gcgtcacttc cctgtttaat attcaatgat
    19681 gacatggggt ataacctgaa aatgagtcca ataggggaaa atctccacgg cgctcacttt
    19741 accagttggt gagaattatc cgaagctgaa gtttgtaaat tccttccact gaatgcaata
    19801 ttcactctat ttaagagaaa tattcatcaa acctataaat caaacaaata aaaaacccat
    19861 aattattttc tgtaattatg ggtttataga taatattaat ttacctgttt agcgttgcgc
    19921 gaataatcgg cccaattttt acaaacgccc ctggagagat aatatccaca tgcgcacaat
    19981 cctgacgata gatatccagc tccgcaatcc acggcgacca ggcgcgttca ggactcatac
    20041 cttcctgaag cgtgcgttca gcaacaaaca acgtcgcttt accgtcaaac ggtacgctat
    20101 gagcagtcgt cagcaggcgc acagcatcag cgtagttgcc ttcaatggtg gtaaacaact
    20161 ccgttgaagt acttccctgc tgtgccgcca ggaaggcctc gcgctcgcgg ttaatctccg
    20221 ccagcacttc cgggtccaga ccattagctt ctttttcctg ccagttttgc gtttctggcg
    20281 gccaggtatc cagcaagcca agaaatgcca cctgttcgcc acgggcacgc agtcgcgccg
    20341 caataccctg cgccagcgta ccgccaaggg aataccccag caggtaataa gggccgtgcg
    20401 gttgttgctc aagtaacgtt gccagatgcg cttcgcagac ttcatccagg tttgtcgctg
    20461 tctgcatggg tccatgaggg cgcggcgact gaatgccaat aatcgaccat tgtggatcga
    20521 gataacgcga gagtacgcta aactgccagg caaaaccgga cgccggatgg aaacaaaaca
    20581 gcgtcgggcc attaccttca cgcaacggca gaatggtttc gaatcccatg cgccgggagc
    20641 tgtcctcttc accatcaata atcgttgcca gtttggcgac ggttgacgcg accatcacct
    20701 gccccggcgt cacctgacga gcaaactgtc gacttaactg cgctgccagt ttcattgcca
    20761 gtagcgaatg accgcccagc gcaaagaaat cagcatcggc atcctgcacg tcacaaccca
    20821 gcaacgacgc gaatgccgcg gcgataatcg tttcactgcc cgctttcggt gcacgtcccg
    20881 acgcttgtgt cttcagttca ggcaacggta aggctttgcg atccagcttg ccgttggcgc
    20941 taagaggtaa ctgtggaagt tgcagcagaa ccaccggtac catatgtggt ggcaatgttt
    21001 cacgaagctg cgcctgcaat gcgctggtat ccaacggcag gcccgattgc gacaccagat
    21061 agcccaccaa ctgacgcgca tcaccaccag tggctgccgc ctggttaatc acacaggcgt
    21121 gggtaacggc ttgttcgaca tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt
    21181 cgatacgctg cccgcgaatt tttagctgat cgtcactgcg cccgaggtac tccaccgcgc
    21241 cgttatccag ccagcgggca acgtctccgg tacggtacat ccgttcgcca ggagcaaaag
    21301 gatcggcaat aaagcggctg gcggtcagat cggggcgtcc gagatagccc tgcgccagtt
    21361 gaatgccggt aagatagaga tcacccgcca cacccggcgg caccggatgc atcatcgcat
    21421 caagaatacg cagccccatg ttccacaccg gataaccaat cggcacactg ctgccgcgca
    21481 cctgtgccag ttcctcgcca aaagccggat accagctgac atctaccgcc gcttccgtcg
    21541 ggccatacag attatgtaac ggcgcaccgg ttaactgttg ccattcacgg cataaatcag
    21601 ccggtaaggc ttcaccacta cagaaaacct gtttcaacgt cgcgcaactc tggcgagcgg
    21661 tttgcggagt cagcgaggca acaaatgccg ccagcatcga cggcacaaag tgcgtggtcg
    21721 ttacgccata ttcggcaaag aattgctgca tagcgagcgg gtcacggtgc gcttccggtt
    21781 cagccatcac cagttttgcc cctgcgataa acggccagaa aaactcccac accgagacat
    21841 caaaactgca cggcgttttt tgggcaacga catcttcgcc tgtaagcggg tagtgatttt
    21901 gcatccataa caggcggtta acgatagccg tctgcccgac cattacccct ttcggcctgc
    21961 cggtggagcc agaggtaaag atgatataag ccgtgtggtg cggttgtgaa agttgcagcg
    22021 gcgcactgcc ctggggggta agcggggcgt tatagcaaag gcttgttaaa ttgggaacat
    22081 cgctaaagcg cggcagttga tcgtcggtgg taattaacag cgacggacgc gcatcttcca
    22141 gcatcatttt caggcgatcg tccggatagc cggtatccag cggtagccag gccgcgccag
    22201 cttcaactat tgcatggagt gccagggtca aaaagaccga gcgcggtagt gccaccgcca
    22261 cgctgtcccc tggtttaacg ccgcgctcac gcagcagatt cgccagcgcc accacctgct
    22321 cgcgcatttc ccgatagctg aacaggtaac gcgcatctgc cagcgccgga gcatccggtg
    22381 tttttgccgc ttgttctgcc accagcgcgc tcagcgtggt ttctggaatc tcaacctgag
    22441 tggcgttgat ctgcgccagc tgcgcatact cacctggcag cataatatcg acatcgccgc
    22501 acaacagcgc cggatccgcg gcgaactggg caatcagcat tttcaggcgt tcagcatgct
    22561 ggattaacgt tggctcatcg taacgctgtt tattggcgag gatctcaata ctcaaatcac
    22621 cgtgttcatc cgggaacagg gccagttcaa ggtcattaac cggaccggtt gccagggtat
    22681 gggtttgcgc ctgaacacca ggaatatcca gttggtaatc aaatacctta atattgagta
    22741 ccggaccaaa cagcggttca tcacctgccg ctcgcccgct gtcacggaca atttgttcgg
    22801 catcgtaacg ttgatggcga cgcatttttt tcagttgagc cgccagtcgg gttgccagtt
    22861 ccggcagcgt ttcttgcgcc gcaatgtgaa tacccaacgg taaaacgttg agcacgggtc
    22921 cgctagccgt cagcgccgcc gagcccagtc gacgcataaa gataaatccg gcggcatagt
    22981 ccatgcgatt gcacaatcgc cccagccaca aggctgccag cgcaagggct aaatcggtac
    23041 gctgcacacc tgaaagttgc gtagccagct ggcggaattc cccgtcggta aattccagtt
    23101 tcaggcgcag aatatctgcc gaagcgctgc gccccggtaa aggtgccgga gaaagtgacg
    23161 cgggcggcgg cagttgacga cgctgttctg cccagaatgc cgcatcacgc tgccaggctt
    23221 cgctttcgcg gtattgctgg tactcttcca ctacatcagc gaaaggcgta aacggcgaag
    23281 caggcgttgg ttcgccacgc agccatgtgc agtaaatatt ggcgatctgg cgagtaatgg
    23341 ccgggaaact gaagccatcg accagcaaat ggtgataacg ctgataccag taccagcggt
    23401 tatccgcaac atgtatcagc tgatgaaaga ccagtggttt accgctatcg acgcgcaaat
    23461 cttgttgcaa atccgtcagc attaatgcct gcgcagtacc gtgcggatca atattggttc
    23521 gcaggtcgat aatttctggc tgttcgaata tcagcgcatc atcgacccac tgccagactt
    23581 cgccgttatc ttccgtaaaa cgcatccgca acgtatctgc ttgcgctagt ccggcaacca
    23641 ccgcgcgggc cagtaatggc gcatcaacat ctccagttaa ctcaacgtaa tgcgccacgc
    23701 tccaggcgga gggtaaatcc gacagttttt ctgccatcca gatgccgggc tgtgcggcga
    23761 ccaaaggtaa atgctggctc attgtgcctc ctgcaactgg gtgaaattgg ccggtgtcag
    23821 agtacgccag tggacttcca gccactgctg gcaggacgcc tgtgactgcg gctgacacac
    23881 aatgtcccag cctgccgata agaggcattg ttgcggccac agactgaatt gcccctgcgc
    23941 attgcgcaaa atgtaaaacg ctccctgcgg atcatcgaag ggattactga atgccatact
    24001 caactcctgt catggaaaag tggttgccag aggtcgatta acccctgcat caagccaccg
    24061 cgccaacaaa gcgcatcatg tccgccgtca acctgacgcc agaaaatgga ttcttttata
    24121 gggtgcaatt gcgcatacag cgcctgattg gcccgcatga tcatcggctc gcgaataccc
    24181 gcttccagca caatgcgcag accttcggcg ctaacttcac cagcttttag cttttcaagt
    24241 aacacgccct cttgctgccc gtcccgatgc ggccaccagt acgatcctga ctggcttaat
    24301 acacagccaa agcgttcagg ccagtgcagt ccggcataca gcgcggacag cccaccaaaa
    24361 ctctgcccgg cgaccacggt gcgatcggcg cgatcgctaa aaggggcaat agctttcacc
    24421 aggggtaata actcttgctg tactgccagc cagaaatccg cattacacgg cagttcgtgg
    24481 gcgcggtgcg tggtgtcgat agcgtcgatc aacacataca cggcgggagg aagttgctga
    24541 cgatgggtca gcgaagtcag cacaggccag acgggcatac tttgcgccca aaattcgcca
    24601 tcgagcaaaa ctgccagcgg acgttcttct gctgttacat cgccggtggt aaaaatccat
    24661 acacgacgtg aatttttcaa ccgttcactt ttccagataa tttctttggc tgctatttct
    24721 ggcacttgcg gacaatccca tcccggttgc agaggcgctt gcggcatttc gagtgcagaa
    24781 acagcgtgcc ctcgcccgcc tttccagctt tgtggattca gcggatcggc tattgcctgg
    24841 ggtaatagtt ttcgccagcc ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa
    24901 atgtcatcgc gttcggtggg aataaagcag tagctgccgc gccagttggc attgagttgt
    24961 gtcgtccact gccagacatc agtacctgca attcgctgca tcgactgggg ctggctgttc
    25021 tgatgatgat cggtcacacc agtgatgtag atccatacgc gctttatcgt cgagtattct
    25081 tcagaacctt ggggatcacg ccaccagaaa gtgacctcaa acatttcgtc attcagacgc
    25141 tgccattccg ggccatgttt cgactgccac cagctctcac ttcctacttt taacgccgtc
    25201 acaccataac cccatgttta ctgtgcaatt tttcattgat tgcagaaata tattgataat
    25261 attattgata accatttgca tttgcaatag cgtaatggcg cgccgtggga agcgcggaca
    25321 tttattaacc aactgcactg cgtgtctttc aggatcaaag gttttcgcgg tagcgggatg
    25381 cgtcgtgttg aggacgacca tgcccgacag ttgcaattcg tggcaaaaat gcaggaataa
    25441 aacaatgaac aagaagattc attccctggc cttgttggtc aatctgggga tttatggggt
    25501 agcgcaggca caagagccga ccgatactcc tgtttcacat gacgatacca ttgtcgttac
    25561 cgccgccgag cagaacttac aggcgcctgg cgtttcgacc atcaccgcag atgaaatccg
    25621 caaaaacccc gttgcccgcg atgtgtcgga gatcatccgt accatgccag gcgttaacct
    25681 gaccggtaac tccaccagtg gtcagcgtgg gaataaccga cagattgata ttcgcggtat
    25741 gggtccggaa aacacgctga ttttgattga cggcaagccg gtaagcagcc gtaactcggt
    25801 gcgtcagggc tggcgtggcg agcgcgatac ccgtggtgat acctcctggg tgccacctga
    25861 aatgattgaa cgtattgaag ttctgcgtgg tccggcagct gcgcgttatg gcaacggcgc
    25921 ggcgggcggc gtggttaaca tcattaccaa aaaaggcagc ggtgagtggc acggctcctg
    25981 ggacgcttat ttcaatgcac cagaacataa agaggaaggt gccactaaac gcaccaactt
    26041 cagcctgacc ggtccgctgg gcgacgaatt cagcttccgc ttgtatggca acctcgacaa
    26101 aacccaggct gacgcgtggg atatcaacca gggccatcag tccgcgcgtg ccggaacgta
    26161 tgccacgacg ttaccagccg ggcgcgaagg ggtgatcaac aaagatatta atggcgtggt
    26221 gcgctgggat ttcgcgccat tgcaatcgct ggaactggaa gcaggttaca gccgccaggg
    26281 caacctgtat gcgggcgaca cccagaacac caactccgat tcctataccc gctcgaaata
    26341 tggcgatgaa accaaccgtc tgtatcgcca gaactactcg ctgacctgga acggcggctg
    26401 ggataacgga gtgaccacca gcaactgggt gcagtatgaa cacacccgta actcgcgtat
    26461 tccggaaggt ctggcaggcg gtaccgaagg gaaatttaac gaaaaagcga cacaggattt
    26521 cgtcgatatc gatcttgatg acgtgatgct gcacagcgaa gttaacctgc cgattgattt
    26581 cctcgttaac caaacgctga cgctgggtac ggagtggaat cagcaacgga tgaaggactt
    26641 gagttccaat acccaggcgc tgaccggggc gaacaccggc ggcgctattg atggtgtgag
    26701 tgccaccgac cgtagcccgt attcaaaagc agaaattttc tcgctgtttg ccgaaaacaa
    26761 catggagctg actgacagca ccatcgtaac gccggggctg cgtttcgatc atcacagtat
    26821 tgtcggcaat aactggagcc cggcgctgaa catatcgcaa ggtttaggcg atgacttcac
    26881 gctcaaaatg ggcattgccc gtgcttataa agcaccgagc ctgtaccaga ctaacccgaa
    26941 ctacattctc tacagtaaag gtcagggctg ctatgccagc gcgggcggct gctatcagca
    27001 aggtaacgat gacctgaaag cagaaaccag catcaacaaa gagattggtc tggagttcaa
    27061 acgcgacggc tggctggcgg gcgtcacctg gttccgtaac gattatcgca ataagattga
    27121 agcgggctat gtggctgtag ggcaaaacgc agtcggcacc gatctctatc agtgggataa
    27181 cgtgccgaaa gcagtggttg aaggtctgga aggatcgtta aacgtaccag ttagcgaaat
    27241 ggtgatgtgg accaataaca tcacttatat gctgaagagt gaaaacaaaa ccacgggcga
    27301 ccgtttgtcg atcatcccgg agtacacgtt gaactcaacg ctgagctggc aggcgcggga
    27361 agatttgtcg atgcaaacga ccttcacctg gtacggtaag cagcagccga aaaaatacaa
    27421 ctacaaaggt cagccagcgg ttggcccgga aaccaaagaa atcagtcctt acagcattgt
    27481 gggtctgagc gcgacctggg atgtgacgaa gaatgtcagt ctgaccggcg gcgtggacaa
    27541 cctgttcgac aaacgtttgt ggcgtgcggg taatgcccag accacgggcg atctggcagg
    27601 ggccaactat atcgcgggtg ccggggcgta tacctataac gagccgggac gtacgtggta
    27661 catgagcgta aacactcact tctgatgcta acgtcagatt gttgacaaag tgcgcgtcgt
    27721 tcatgccgga tgcggcgtga acgccttatc cggcctacaa aaatcttgcc aattcaatat
    27781 attgcaggac catgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcatc
    27841 agtctcgaat atggtcgata tgaaaactac gcatacctcc ctcccctttg ccggacatac
    27901 gctgcatttt gttgagttcg atccggcgaa tttttgtgag caggatttac tctggctgcc
    27961 gcactacgca caattgcaac acgctggacg taaacgtaaa acagagcatt tagccggacg
    28021 gatcgctgct gtttatgcct tacgggaata tggctataaa tgtgtgcccg caatcggcga
    28081 gctacgccaa cctgtctggc ctgcggaggt atacggcagt attagccact gtgggactac
    28141 ggcattagcc gtggtatctc gtcaaccgat tggcattgat atagaagaaa ttttttctgt
    28201 acaaaccgca agagaattga cagacaatat tattacacca gcagaacacg agcgactcgc
    28261 ggactgtggt ttagcctttt ctctggcgct gacactggca ttttccgcca aagagagcgc
    28321 atttaaggca tcggagagac aaactgaagc aggttttctg gattatcaga taattagctg
    28381 gaataaacag caggtcatca ttcatcgtga gaatgagatg tttgctgtgc actggcagat
    28441 aaaagaaaaa atagtcataa ctctgtgcca acacgattaa ttgacaacat ctggtacgat
    28501 tcgcccgcag ccatcactga ctgcgggcga aagtgtaaag caggtgcctt accactctga
    28561 cctgacaacc ggatatgcag ggattgctcc ccgcacaacg gctacttctt cggttcgtaa
    28621 gcgagaacag ccttaaactc aatattacgt tcctttacgg tgaactcaca cagcgagtct
    28681 ccgaccagaa gcgtaaatcc cagcaccgtc aaacacagca ctatgactgc cacaagggca
    28741 tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat ccgagttgtt
    28801 gaggttcgga gtggcagccc caggattgat agaaatatct cctggggctt catctttcca
    28861 gacctcagag taaacctgaa accggaaagc ttcaggcacc cgccgttatc ttacccttcc
    28921 tcagcaaaat atctgtttat ttatacagta agtcctgttt cggacttctg ttggcaacac
    28981 tcgttattgc tcgtaaaacg aaatcgcccg cagccatcac tgaccgcggg cgaaagtgta
    29041 aagcaggtgc cctaccatcc tgacctgaca accggatatg cggggattac tccccgcaca
    29101 acggctactt cttcggttcg taagcgagaa cagccttaaa ctcaatatta cgttccttca
    29161 ccgtaaactc acacagcgag tctccgacca gaagcgtaaa tcccagcacc gtcaaacaca
    29221 gcactatgac tgccgcaagg gcatatttcg tcagcatgtt tatattgcct ccatgcgaag
    29281 aagggactac catccaaatt gttcgggttt ggagtggcag ccccagggtt gatagaaata
    29341 tctcctgggg cttcatcttt ccagacctca gagtaaacct gaaaccggaa agcttcaggc
    29401 acccgccgtt atcttaccct tcctcaacaa aatggctgtt tatttataca gtaaatcctg
    29461 tttcggactt ctcactcctc atttaatcaa ctgaagctga gcccgttttt tctgcatgcc
    29521 tcaatcgtca aaattcttcg ccatcagcca gctctgccat aacttgccac attgaaaaag
    29581 tagccaaatt gcttcagtca ccatcatcaa aaaaagaaac attccagaac ttccgcatct
    29641 cgcccagccg ctccgacttc tatactgaat agaaaacgcc aacataagag aaacctatgc
    29701 cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg gaaatgcagg
    29761 tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt gacgcggtta
    29821 aaaataagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg ctggagctgg
    29881 cgacggatgt ttgccgtgat atcaaccagg ctgccgggca attttcagcg atgcagaaag
    29941 tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc acacacccgt
    30001 ttcagaaatg gcagcgtcag gaggtatgcg ataacgagcg ctatcaacga acgctggaaa
    30061 actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat gttggctgcg
    30121 ccagtggcga tgacgccatt tatttgctgc acggcttgtc acggtttgtg ccgcacttta
    30181 tcgccctttc cgccgcgtcg ccatatatgc aaggaacgga tacgcgtttt gcctcctcac
    30241 gaccgaatat tttttccgcc tttcccgata atggcccgat gccgtgggtc agtaactggc
    30301 aacaatttga agccctgttt cgctgtttga gttacaccac gatgatcgac agcattaaag
    30361 atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt cgggtgatgg
    30421 ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag gctaccgccc
    30481 actggttact gacagaacgc ccgttcaaac atcaggagaa agattacctg ctgtataaat
    30541 tcaaccgttt ccaggcctgt cgctatgggc tggaaggcgt cattaccgat ccgcacactg
    30601 gcgatcgtcg accgctaacg gaagacacct tgcgattgct ggaaaaaatc gccccttccg
    30661 cacataaaat tggtgcatcg agcgcgattg aggccctgca tcgccaggtc gtcagcggtc
    30721 tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg tggctcgctg attgggctgg
    30781 tgaaaaagca ttgtgagatc tgggccgatg actaagtagg gaattgcctt acagaagaat
    30841 atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc ctttagaaac
    30901 cttgactacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc tgctgcccgc
    30961 ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc tgatggatct
    31021 tagctagctt tacactttat tgttttgtct gtggttttta gtgctgggcg ctattgagta
    31081 ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa cgtcgattat
    31141 cgccgtactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg tttatggata
    31201 agcaatcact gcacgaaacg gcgaaacgcc tggcccttga gttacccttt gtcgagcttt
    31261 gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaaaatt tttatgctgt
    31321 cgtcggagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa aaatccctgt
    31381 taaatcagca gatatacccg agcattaagc cagggtatca catgaataaa aagcactgga
    31441 tttcggtgta tcccggcgag gaaatctccg aagcgttact tcgcgatctg atcaacgatt
    31501 cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg cgtccaggct
    31561 aaagcggaaa tctatagcgc atttttctcg cttaccattt ctcgttgaac cttgtaatct
    31621 gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct gtcgccttaa
    31681 agcgtcattc cactaaggca tttgatgcca gtaaaaaact taccccggaa caggccgagc
    31741 agatcaaaac tctcctgcaa tacagcccat ccagcaccaa ctcccagccg tggcatttta
    31801 ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc ggtaattatg
    31861 tgttcaacga acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt gcaaaaaccg
    31921 cgatggacga tgcctggctg aagctggttg ttgaccagga agatgctgat ggccgctttg
    31981 ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgct gatatgcacc
    32041 gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc aacgtcggta
    32101 acttcctgct cggcgtggcg gctctgggtc tggacgcggt acccatcgaa ggttttgacg
    32161 ccgccatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt ctggtggttg
    32221 ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa tctcgtctgc
    32281 cgcaaaacat caccttaacc gaagtgtaat tctctcttgc cgggcatctg cccggctatt
    32341 tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata ggctgctgtt
    32401 gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag cgggtattga
    32461 gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga ccgccgtcgt
    32521 ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg ccatcgccag
    32581 ttcacggctt tggttgcaaa tcataaccca gaataaactc ttccaccgtt tagcttttac
    32641 cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag gcaccgaagc
    32701 ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc tgctttcagt
    32761 cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa ttcccggcgg catctcagtt
    32821 accgctgaaa gggatatttc aggggattaa actgatcggc gcgatcctgg tcggcatttt
    32881 gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc ttggtgcaat
    32941 ggctgccgtg ctgatgttgg tatttaaaga tccgattctt ggtctggtgg ccggtattca
    33001 gctttccgcg aacgatatgc tgaaactggg cgactggctg gagatgccga aatacggcgc
    33061 agatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact gggacaatac
    33121 cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact ggagcgggat
    33181 gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca ccagtattcg
    33241 ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg catttgttaa agccttattt
    33301 aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta cggagtcggt
    33361 attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga acgaatatct
    33421 gcgtaaccat ccgcggattc gtaaagatat gaccttaatg gtacgccaac tggctccagg
    33481 tgataacggt ttaccgctcg agatctacgc gtttactaac accgtggtgt ggctggaata
    33541 tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag agtttggtct
    33601 gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg catttaagca
    33661 gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt gtcggatgta
    33721 gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa tacaatccac
    33781 accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag cagcaaaatc
    33841 atgccgagga aggcaatgca gagatagttt ccgaacggat agagcagcgc cttaaactgt
    33901 gtttcacgcc cctgacgtcg catcgctgca cgaaaacgca gatgcgccag acagatcata
    33961 atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa cgctttttgc
    34021 ggcagcagat agttgattaa caccaccagc gaagtgatcg ctccggaaag catcagcgag
    34081 ttaatcggca caccgcgacg gctgacgcga gtcaaaaact tcggcgcatt accctgcaca
    34141 gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac tgacagcgat
    34201 gctaccagaa tgacgaagtt cagcgcagaa gctaccacgt tgctgtcgag attatggaaa
    34261 atcatcacaa acgggctact gttggatttc acttccaccc acggatagag cgccagtaaa
    34321 accaccagtg aaccgatgta aaacagcagg atgcgataca ccacctgatt taccgctttt
    34381 ggaatgcttt tatccggatc gcgcgcttca gcggcagtaa tcccaatcag ctccagaccg
    34441 ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccagcc ggtggcgaag
    34501 aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgtg accagaaaac
    34561 agcagccaca ggccaaagcc gatcataccg atgattgcca gcactttaat caacgcaaac
    34621 cagaactcgg tttcgccata taagcgcacg ttcaccaggt taacggcgtt gatgataata
    34681 aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg catatagatg
    34741 cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta gttccagcca
    34801 gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa tgaaccggat
    34861 accggctctt cgaccaccat ctcgccaagc tggcgcataa tcaggaaagc gatgatcccg
    34921 gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg accaatgcca
    34981 agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg acggttatgt
    35041 aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac ggttgacgcg
    35101 tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta acatttagtg
    35161 cccatcgtcg caagacacaa tccacacggt taaacggggt atcctgcttt tatttccgta
    35221 cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac agcgacagca
    35281 aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat ccggctcatc acctctgaac
    35341 cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc accgtcatcg
    35401 ctttcgggcg cacgcgcagt accgcgccgt gatacaacgc ctcatccagc ttctgctcac
    35461 tgaatgtttg cggattattc aacgacggct cggcctctat ggcgtgacgt aaatacatca
    35521 gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaaaccg gtgcccgttg
    35581 ccacagaaag atgaaagccc atccaccaca gcaaccagat gccgcccacc agcgcaaacg
    35641 gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaatgccaga tacaacagca
    35701 cgaagatgat catcagtgtc atcggcacca tgagtttcag cttatggttg gcgcgctcca
    35761 gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc actttttcag
    35821 ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg gcatcgatat
    35881 aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg gtagagactt
    35941 taacgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg atcggtagct
    36001 ggcgcagtgc ctgcgggcta tcgcgccagc tttgcggata acgcagatta atgggataac
    36061 gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa gtcacaaaca
    36121 actgcacatc cgccaccgtc ataccgtaac gcgcggcttt ttcacggtta atctcaacgt
    36181 tgatatagcg cccaccttcc agacgctcgg ctagagcaga agccacgctt ggcaccgttc
    36241 gcgctacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca gtgccggaaa
    36301 ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg ttacgaattg
    36361 gcggcaccca cagattcgcc agccccggca gccgcacggt gttatccagt tcctcaatga
    36421 ttttgtccat cgtcatgcca ggccgccact gatcctgcgg cttaagctgg atggtcgttt
    36481 caaccatctc aagcggagca gaatcggtgg cggtttccgc tttcccggtt ttgccaaata
    36541 cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc atactcgccg
    36601 cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg ccttcattga
    36661 tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc agcaccgaaa
    36721 gcgctgccac caacagcgtg gttttcggcc agtgcagtac tttcagcaat agaggatgat
    36781 aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt ttgccacgga
    36841 tccagtaccc catcaggata gggatcacta cgatcgccag cagcgccgcg cccgccatcg
    36901 cataggtttt agtgaacgcc aacgggccaa acagacgccc ttcctgccct tccagggtga
    36961 agatcgggat aaacgacaac gtgataatca gcagactgat aaatagcgcc ggacccactt
    37021 caacagatgc atcggtgatc acctgccagc gcgttttatt atccagcgtg gcgtcaggat
    37081 gctggtgctg ccactcttcc agccgtttat gtgcattttc gatcatgacg atagcggcat
    37141 cgaccatcgc cccgacggca atcgcaatgc cgcccaacga cataatattg gcattcagtc
    37201 cctggaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa ataatcgcca
    37261 ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc acgataaact
    37321 cttccagcaa cttgccgctg aggttgtcga tggcgcggtc aatgagctgg ctgcgatcgt
    37381 atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc agtttgtcct
    37441 tcacggaggc gatcacttct cgggcgtttt tgccggagcg caggatcacc accccgcccg
    37501 ccacttcgcc ttcgccattc agttcggcaa tgccccggcg catctccggg ccaatctgga
    37561 ccttcgcaac atcacgcaga taaacgggca cgccattttc actggctttt aaaacgatgt
    37621 gattaaagtc gtcgagcgtt tgcagatagc cgctggcgcg caccatatat tccgcttccg
    37681 ccagttcgat cgacgaaccg cccgcttcct ggtttgaagc atccagcgcg ctttttactt
    37741 cggctagact gatgccatat tgcgccaggc gctggggatc gataaccacc tgatactctt
    37801 tcaccacgcc gcccaccgac gccacttccg caacgtcagg gatggttttt agctcatatt
    37861 tgagaaacca gtcctgcaat gagcgtaaat cggccagatc gtgcttaccg ctgcgatcca
    37921 ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggtcccagc tcggcgctga
    37981 ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc gaacgcgccc
    38041 agtacggatc ggtaccatct tcgaaaatga catacacata agagtctcca aactgcgaga
    38101 aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc ggataagtca
    38161 cctgattttc aacgatttgc ggtgcctgac cgggatagct ggttttaata atcacctgca
    38221 catcggagag atccggcagc gcatccactg gcgtgttaat gatggtccag gtgccccaga
    38281 tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag cgacgaataa
    38341 tccattcaat cattggttat tccctcaatg cgcattggta gcactttcag agcgcatccg
    38401 ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc tggaaaccac
    38461 cttttcacct tccgccagac cagagtgtaa cgcggtgacg ccttgcgatg cctggaaaac
    38521 ggcaacgcgt ttcggtacaa agcgtccgtc ggcatcaacg gtaatcaccc gctgttcgtt
    38581 gccggtatca atcagcgcct gtgacggaat gagcagcatc ggttcgctgg cggtgttgag
    38641 ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga cttccagacg
    38701 cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt tgcggatggt
    38761 gagtgtttta tccggtcgcg ccggaacggt gagcgtaaac tgcgaggcat ctttcaccag
    38821 ccaggcgata gactccggga tcgcagcagt gacccacacc gggtccatac cctgaatttt
    38881 cgctaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg cggtgatcac
    38941 gccatcaatg ggtgctttga gcgtaaagcg agtctggatt ttttgcgtgg cgatcaggcg
    39001 gcgaatatcc gcctccggca ttcccgccag tcgcagccgc tcaagaatgc cttcagtctg
    39061 ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt ccacccagtc
    39121 aggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca cggtaagcgg
    39181 atacaccttg tcgataaacc cggcagcgcg agcctgcaca atggcatact gatactcgtt
    39241 gtaactgaca ttcgccggga aactctgggc aaaagtcagc ggtccgcgcg tgacggtagc
    39301 cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag acgcagaact
    39361 ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt tacctggttt
    39421 atcgaaccgc gtattggggt acatcgggtc gtaccagaat aagattttac gttctgcggt
    39481 cgacgttttt tctgcgggtg gttcagcctt tgcaaaccag gtaaaacctg ccgcagaaat
    39541 aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct cattaaacct
    39601 gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct ggacaaaatt
    39661 aaacgccact ttgtcgccgg ttttaatttc actcattttc gtctgcgggg tgatggtaaa
    39721 gcgcatagtc atctccggcc agttcacggc agcaatcgga tcgtgatgga tggtgatttt
    39781 tttgctttcc agatcaatac cctttaccac gccagtggcg ctaataacct gcggttgtac
    39841 ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa taacggtaaa
    39901 cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg agttaaaaat
    39961 tattgctgcc aaccgccccc taaggcggta tacaaagaaa tttcgttaac ctgacgggca
    40021 taattcaggt cgagtaaagt ttgtcgggtt gcaaataaag aacgctcggc atccagcact
    40081 tccagataac ttaccgcgcc gtgctgatat aatgcccgcg cccgttgcaa agtaatttgc
    40141 agcgacgcca gataacgctg ctgggcgctg atttgatcgt tcaggctttg acgtaatgcc
    40201 agcgcatctg ccacttcttt aaaggcgttc tggattttct gttcataatt cactaccgac
    40261 tgctgctggc gaatttcggc gatatccaga ttggcctggt tgcgtccggc attaaaaatg
    40321 gggatctcaa ttttaggaat aaaattccac atcccgctgc tagcgttaaa taatgaagac
    40381 agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa aaatgccgca
    40441 cgcgcagcac caatattagc attagccgcc attaacgcgt gttcagcttc cataatatcc
    40501 gggcgctgca ataagatttg cgacgacaag cccgccggta atttaacgct ttgcaggctg
    40561 ccgctgttta ctgtctgcgc ttgcggcagt ttgccgtagc ttcccaataa cagttgcaat
    40621 gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcactgcg ggtactttct
    40681 atcaccccgc gagcctgttc cagcgccaga acattgctgc taccggtcaa cagttgtttt
    40741 tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat ttgcaattgc
    40801 gcatacgcca gttgctgatt gaaatagctt tgcgcgacat tagaaaccag cagaatatgc
    40861 accgcgcgct gagcttcctc agtggctaaa taattttgtc gctcggcttc gctcatgttc
    40921 tttaagcgac cgaaaaaatc gaggtcaaag ctggcgttaa ggccggtcga aaactcccgt
    40981 gtcgtggcgg tattcccttt aagattaccg ctccagctgc cgctgccctc gccattgagc
    41041 tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gtgcctcctg cactttcagc
    41101 gtcgccatgc gcaaatcccg gttattcacc agcgcctcgc taatcagcgt cttcacctga
    41161 ttatcaacaa aaaaggtgcg ccagcccacg ttctggtagt tatctgctgc gttaaccaag
    41221 ccgttctggc tgagtgagaa ctgctgcggc acgggcattg ccgtacgctg ataatccggt
    41281 gccaatgaac aaccggttag cgcaagggcc acacaaaatg gcagaagttt acaaggagac
    41341 ataggctcat aatttctggt gattttatac cgccaacttt actcgccagg ctctgatttt
    41401 ccggtgacag gaaaatgaca aaattgtcat tttgccaata agcgattgcc atctgatccc
    41461 gctactctag aattgcccgg gcaacatgcg gaggaaaaat gaaactgttg attgtcgaag
    41521 atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt tttgtggtcg
    41581 atttggccga caacgggctg aatggctacc atctggcgat gaccggtgat tatgacctga
    41641 taatcctcga tattatgctg ccggacgtga acggctggga tatcgtgcgc atgttgcgct
    41701 ccgccaataa agggatgccg attctgttgc ttaccgcgct tggcaccatt gaacatcgcg
    41761 tcaaggggct ggagttgggg gccgatgact atctggtgaa gccgttcgct tttgctgaac
    41821 tgctggcgcg ggtgcgcact ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt
    41881 ttcaggttgc cgatctgatg gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc
    41941 gcatcactct gaccagtaaa gagtttactc tgctggagtt ttttctccgc catcagggcg
    42001 aagtgctgcc ccgctcgctt atcgcctcgc aggtgtggga catgaatttc gacagcgaca
    42061 ctaacgccat tgatgtggcg gtgaagcggc tacgcggcaa aatcgacaac gactttgagc
    42121 cgaagctgat tcagaccgtg cgcggcgtgg gctatatgct tgaggtgccg gatggtcagt
    42181 aagccatttc agcgcccgtt ttcgctggca acccgtctga ccttttttat cagcctggcc
    42241 accatcgcgg cgtttttcgc ctttgcatgg atcatgatcc actcagtgaa agtgcatttt
    42301 gccgagcagg atattaatga tttaaaagaa attagcgcca cccttgaacg ggtacttaat
    42361 catcctgacg agacgcaagc ccgacgctta atgacgctgg aagatatcgt cagtggttat
    42421 tccaacgtgt tgatttccct ggcagatagc cacggcaaaa cggtgtatca ctcccccggt
    42481 gcgccggata tccgcgagtt tacgcgtgac gccatacccg ataaagacgc tcagggtggc
    42541 gaggtgtatc tcctttccgg cccgacgatt atgatgccag gccacggtca cgggcatatg
    42601 gaacacagca actggcggat gattaacttg ccggttggcc cgttggtgga cggcaaaccg
    42661 atttatacgc tctacatcgc gctttcgatc gattttcatc ttcattacat aaatgatttg
    42721 atgaataaac ttattatgac cgcatctgtc atcagcatcc tgatcgtctt tatcgtgcta
    42781 ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc
    42841 acctcgaaag atctcgacgt tcgccttgac ccgcagaccg agcccattga gctggaacag
    42901 ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac ccgccagtcc
    42961 aatttctcag cggatatcgc tcacgaaatt cgcacgccga ttaccaatct catcacgcaa
    43021 acggaaatcg ccctcagcca gtctcgcagc cagaaggagc tggaagatgt gctctactct
    43081 aatctcgaag agctgacgcg aatggcgaaa atggtcagcg atatgctgtt tctcgctcag
    43141 gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga tgaagtcggc
    43201 aaagtgttcg attttttcga ggcgttagcg gaagatcgcg gcgtggagtt gcgatttgtt
    43261 ggcgacaaat gtcaggttgc gggcgatccg ctgatgctgc gtcgggcgtt aagcaacctg
    43321 ctttctaatg ccctgcgtta tacgccaacc agagagacaa ttgtagtgcg ctgtcagacg
    43381 gtcgatcacc aggtgcaagt taccgtcgaa aaccccggta cgcccattgc gcccgagcac
    43441 ttaccgcgat tgtttgaccg tttctaccgc gttgaccctt cccgccagcg aaaaggcgaa
    43501 ggtagcggca ttgggctggc gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt
    43561 gcagtaacgt cagatgcgcg ggggacaagg tttgtgatta tgctgccgga gagagagtga
    43621 ttattaagtt caaattcagg gcgtggtgca tcacctcctg gtattgttga tcaacaaatt
    43681 agagaaaaac ttattaaaca tatgaaatat cgaaggcttt tatttttcaa agtttcacga
    43741 gatgtttgta tatgaaactt agttaaatct gtgtttcttc caattgtaga tcacaatgcc
    43801 ctttctaaat tttcatcgtt a
//
LOCUS       DAZPBM010000016        43399 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPBM010000016 DAZPBM010000000
VERSION     DAZPBM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.016"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(2..94)
                     /locus_tag="ACHUZQ_000777"
     tRNA            complement(2..94)
                     /locus_tag="ACHUZQ_000777"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHUZQ_000778"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHUZQ_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1024556.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHUZQ_000779"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHUZQ_000779"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1024557.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHUZQ_000780"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHUZQ_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1024558.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4316..5377)
                     /gene="recA"
                     /locus_tag="ACHUZQ_000781"
     CDS             complement(4316..5377)
                     /gene="recA"
                     /locus_tag="ACHUZQ_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1024559.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="ACHUZQ_000782"
     CDS             complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="ACHUZQ_000782"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1024560.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="ACHUZQ_000783"
     CDS             complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="ACHUZQ_000783"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1024561.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7440..8003
                     /gene="srlA"
                     /locus_tag="ACHUZQ_000784"
                     /gene_synonym="gutA"
     CDS             7440..8003
                     /gene="srlA"
                     /locus_tag="ACHUZQ_000784"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR1024562.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8000..8959
                     /gene="srlE"
                     /locus_tag="ACHUZQ_000785"
                     /gene_synonym="gutE"
     CDS             8000..8959
                     /gene="srlE"
                     /locus_tag="ACHUZQ_000785"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1024563.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            8970..9341
                     /gene="srlB"
                     /locus_tag="ACHUZQ_000786"
                     /gene_synonym="gutB"
     CDS             8970..9341
                     /gene="srlB"
                     /locus_tag="ACHUZQ_000786"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1024564.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9345..10113
                     /gene="srlD"
                     /locus_tag="ACHUZQ_000787"
                     /pseudo
     CDS             9345..10113
                     /gene="srlD"
                     /locus_tag="ACHUZQ_000787"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10219..10578
                     /gene="gutM"
                     /locus_tag="ACHUZQ_000788"
     CDS             10219..10578
                     /gene="gutM"
                     /locus_tag="ACHUZQ_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1024565.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10645..11418
                     /gene="srlR"
                     /locus_tag="ACHUZQ_000789"
                     /gene_synonym="gutR"
     CDS             10645..11418
                     /gene="srlR"
                     /locus_tag="ACHUZQ_000789"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1024566.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11411..12376
                     /gene="gutQ"
                     /locus_tag="ACHUZQ_000790"
     CDS             11411..12376
                     /gene="gutQ"
                     /locus_tag="ACHUZQ_000790"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1024567.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12373..13887)
                     /gene="norR"
                     /locus_tag="ACHUZQ_000791"
     CDS             complement(12373..13887)
                     /gene="norR"
                     /locus_tag="ACHUZQ_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1024568.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14074..15513
                     /gene="norV"
                     /locus_tag="ACHUZQ_000792"
     CDS             14074..15513
                     /gene="norV"
                     /locus_tag="ACHUZQ_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1024569.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15510..16643
                     /gene="norW"
                     /locus_tag="ACHUZQ_000793"
     CDS             15510..16643
                     /gene="norW"
                     /locus_tag="ACHUZQ_000793"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1024570.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(16771..19023)
                     /gene="hypF"
                     /locus_tag="ACHUZQ_000794"
     CDS             complement(16771..19023)
                     /gene="hypF"
                     /locus_tag="ACHUZQ_000794"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1024571.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTISQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19176..19703)
                     /gene="hydN"
                     /locus_tag="ACHUZQ_000795"
     CDS             complement(19176..19703)
                     /gene="hydN"
                     /locus_tag="ACHUZQ_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1024572.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19851..20861)
                     /gene="ascG"
                     /locus_tag="ACHUZQ_000796"
     CDS             complement(19851..20861)
                     /gene="ascG"
                     /locus_tag="ACHUZQ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1024573.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21121..22578
                     /gene="ascF"
                     /locus_tag="ACHUZQ_000797"
     CDS             21121..22578
                     /gene="ascF"
                     /locus_tag="ACHUZQ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR1024574.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22587..24011
                     /gene="ascB"
                     /locus_tag="ACHUZQ_000798"
     CDS             22587..24011
                     /gene="ascB"
                     /locus_tag="ACHUZQ_000798"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR1024575.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24125..24544)
                     /locus_tag="ACHUZQ_000799"
     CDS             complement(24125..24544)
                     /locus_tag="ACHUZQ_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1024576.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(24541..24852)
                     /locus_tag="ACHUZQ_000800"
     CDS             complement(24541..24852)
                     /locus_tag="ACHUZQ_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR1024577.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25014..25787)
                     /locus_tag="ACHUZQ_000801"
     CDS             complement(25014..25787)
                     /locus_tag="ACHUZQ_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024578.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(25812..26282)
                     /gene="hycI"
                     /locus_tag="ACHUZQ_000802"
     CDS             complement(25812..26282)
                     /gene="hycI"
                     /locus_tag="ACHUZQ_000802"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR1024579.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26275..26685)
                     /gene="hycH"
                     /locus_tag="ACHUZQ_000803"
     CDS             complement(26275..26685)
                     /gene="hycH"
                     /locus_tag="ACHUZQ_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR1024580.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(26682..27449)
                     /gene="hycG"
                     /locus_tag="ACHUZQ_000804"
     CDS             complement(26682..27449)
                     /gene="hycG"
                     /locus_tag="ACHUZQ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR1024581.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(27449..27991)
                     /gene="hycF"
                     /locus_tag="ACHUZQ_000805"
     CDS             complement(27449..27991)
                     /gene="hycF"
                     /locus_tag="ACHUZQ_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR1024582.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28001..29698)
                     /gene="hycE"
                     /locus_tag="ACHUZQ_000806"
     CDS             complement(28001..29698)
                     /gene="hycE"
                     /locus_tag="ACHUZQ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1024583.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(29716..30639)
                     /gene="hycD"
                     /locus_tag="ACHUZQ_000807"
     CDS             complement(29716..30639)
                     /gene="hycD"
                     /locus_tag="ACHUZQ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1024584.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(30642..32468)
                     /gene="hycC"
                     /locus_tag="ACHUZQ_000808"
     CDS             complement(30642..32468)
                     /gene="hycC"
                     /locus_tag="ACHUZQ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR1024585.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(32465..33076)
                     /gene="hycB"
                     /locus_tag="ACHUZQ_000809"
     CDS             complement(32465..33076)
                     /gene="hycB"
                     /locus_tag="ACHUZQ_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1024586.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33201..33662)
                     /gene="hycA"
                     /locus_tag="ACHUZQ_000810"
     CDS             complement(33201..33662)
                     /gene="hycA"
                     /locus_tag="ACHUZQ_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1024587.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            33874..34224
                     /gene="hypA"
                     /locus_tag="ACHUZQ_000811"
     CDS             33874..34224
                     /gene="hypA"
                     /locus_tag="ACHUZQ_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1024588.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34228..35100
                     /gene="hypB"
                     /locus_tag="ACHUZQ_000812"
     CDS             34228..35100
                     /gene="hypB"
                     /locus_tag="ACHUZQ_000812"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1024589.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35091..35363
                     /gene="hypC"
                     /locus_tag="ACHUZQ_000813"
     CDS             35091..35363
                     /gene="hypC"
                     /locus_tag="ACHUZQ_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1024590.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            35363..36484
                     /gene="hypD"
                     /locus_tag="ACHUZQ_000814"
     CDS             35363..36484
                     /gene="hypD"
                     /locus_tag="ACHUZQ_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1024591.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            36481..37491
                     /gene="hypE"
                     /locus_tag="ACHUZQ_000815"
     CDS             36481..37491
                     /gene="hypE"
                     /locus_tag="ACHUZQ_000815"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1024592.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            37565..39642
                     /gene="flhA"
                     /locus_tag="ACHUZQ_000816"
                     /pseudo
     CDS             37565..39642
                     /gene="flhA"
                     /locus_tag="ACHUZQ_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(39679..40032)
                     /locus_tag="ACHUZQ_000817"
     CDS             complement(39679..40032)
                     /locus_tag="ACHUZQ_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1024593.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40109..40243)
                     /locus_tag="ACHUZQ_000818"
     CDS             complement(40109..40243)
                     /locus_tag="ACHUZQ_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024594.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            40319..42880
                     /gene="mutS"
                     /locus_tag="ACHUZQ_000819"
     CDS             40319..42880
                     /gene="mutS"
                     /locus_tag="ACHUZQ_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1024595.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            42986..43399
                     /locus_tag="ACHUZQ_000820"
                     /pseudo
     CDS             42986..43399
                     /locus_tag="ACHUZQ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT        10273 a        11917 c        11305 g         9904 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatccct acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gagtgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcacca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcagc caattcgtta gaggatcgag
     4201 gtgagagtaa tttttcacgc cagtgttggc ctgttcccct caccctaacc ctctccccaa
     4261 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4321 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4381 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4441 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4501 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4561 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4621 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4681 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4741 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4801 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4861 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4921 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     4981 gggcgtcaca gatttccagt gcctgctcgc cggtgtccgg ctgggagcac agcaggttat
     5041 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5101 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5161 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5221 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5281 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5341 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5401 ataccggata gtcaatatgt tctgttgaag caattatact gtatactcat acagtatcaa
     5461 gtgttttgta gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc
     5521 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5581 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5641 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5701 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5761 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5821 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5881 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     5941 tcactgtcag tcatcacaac ctcagtcagt caaaaattta tgccggacaa gatagcactt
     6001 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6061 tagccagagg gaagctcacg cccccctctt gcaaatagtt actgcacgcg cgccagcgcc
     6121 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt ataacgggta
     6181 atggtgtaga agttcggcag accgtaccag tactggtaac cggtaccgac atccagacgc
     6241 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgccgtggca
     6301 agctgcgaaa tgctgtacct ggttttgaag ccatttggca agcctggagc ctgaccgttt
     6361 gccattaccg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccaca
     6421 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6481 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6541 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcaacaggaa ggtttccagc
     6601 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6661 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6721 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6781 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6841 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6901 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     6961 cgatcgaaac cgtgtttgtt caccattctg tcgatgaact gctgggcatt cgggttatta
     7021 gcgaaatcgc cgcccatctg catcacattg tgctgaggct caagcaggaa gccgccagac
     7081 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7141 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7201 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7261 ctgcccgata cggactttac ataactcaac tcattcccct cgctatcctt ttattcaaac
     7321 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaatctttca
     7381 atctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7441 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7501 aggtgtttac cgggatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7561 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7621 ccggtaaccc tgtttcccgt tacctgctgc taccgtgcat tggcacgttt gtcttttgca
     7681 atccgatgac cttaagcctg ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7741 cggcagcctc ttatagctgc cactcaatga atggcctctt tccccatatc aaccctggcg
     7801 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7861 cactggcggt gagctatctg ctggttggtc tggtcaccaa cttctttcgc ggctgggtga
     7921 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     7981 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8041 cggcccgctt gagctggaag ccacgccggg caaaaaaatc gtctatatca ccgccggtac
     8101 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8161 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8221 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8281 caagtccagt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8341 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8401 tgacaccagc aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8461 tatgggttct gccgttgccg tgttgtttca atctggtcgt gacaccatcg acactgtatt
     8521 aaaaaccatt ctgccgttta tggcgttcgt ctcggcgctc attggcatca ttatggcttc
     8581 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8641 ggtcatgctg gcgctcatct gctccttccc gctgctttca cctttcctcg gcccaggcgc
     8701 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8761 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8821 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8881 tgtactggtg agccactttt taaccggcgc accgactgta ctgatcgcct ggtttgtctc
     8941 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9001 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9061 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9121 cactccatcc cggtttgcaa tttacgctcg ggcagcatcg ctatccggtg accgctgttg
     9181 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9241 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9301 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9361 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9421 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9481 ttaacgccga atatggtgaa ggaacagcgt atagttttgg tgctgacgcc accagcgagc
     9541 aaagcgtcct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9601 tctacagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9661 accgtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttctcgttt gatgatccgc
     9721 gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt gggcagcaaa
     9781 cacaactctg gctacagcgc agcgaaattt ggtggcgtcg ggctgactca atcactggcg
     9841 ctggatctgg cggagtacgg cattacggtg cattcactga tgctcggtaa cctgctgaaa
     9901 tcgcctatgt tccagtcact gttaccccaa tacgcgacca agctgggtat caaaccggat
     9961 caagtcgagc agtattacat cgacaaagta ccgctcaaac gcggctgcga ttatcaggat
    10021 gtgctgaata tgctgctgct ctatgccagt cctaaggcgt cgtactgcac cggacagtcg
    10081 atcaatgtca ccggcggtca ggtgatgttc taatcaacag cggagatccg ttaaggatct
    10141 ccgtgagact aatagaatgc ctgatgcgct acgctcatca ggcatacagg acttccgcca
    10201 ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt
    10261 gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt cgacacacta
    10321 tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc gctttaaacc acgggtcgtg
    10381 gtcgccatcg ccctggacga tcagcagcgc atcgtcgaca ccttgtttat gaaaggactg
    10441 accgtcttcg cacgaccgca aaaaattccc gcaattaccg gtatgcatgc gggtgattta
    10501 cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc attggcgctt
    10561 aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga
    10621 aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc cattctggag
    10681 tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt tgacaccaca
    10741 ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg caggaaccgt cattcgtact
    10801 tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca taaaacactc
    10861 atcaacaccc acaagaaaga gctgatagca gaagctgccg ttagttttat tcatgatggc
    10921 gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct gctctcgcgc
    10981 tttaataaca tcacggtgat gaccaacagt ctgcatatcg tcaatgcgct atctgaactg
    11041 gataacgaac aaactatcct gatgccaggc ggaacgtttc gtaaaaaatc ggcctcattt
    11101 cacggacagc tggcagagaa tgccttcgaa catttcagtt tcgataaatt gtttatgggc
    11161 accgacggca tcgatctcaa tgcgggcgta accaccttta acgaggttta taccgtcagt
    11221 aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc aaagtttggc
    11281 cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctgat taccgacgca
    11341 ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt gatcataacc
    11401 ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa tgctggagtt
    11461 gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg ccgccaatat
    11521 catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg
    11581 taagaaaatc gccgcaacac ttgccagtac cggcacatcg gctttttttg tccatccggc
    11641 agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc tgtttatctc
    11701 ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag ataaatctat
    11761 cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg caaaagcggt
    11821 gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga cctccagcac
    11881 cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg cgcgcggatt
    11941 taatgaagaa gattttgccc gctcccaccc agccggggca ctgggcgctc gcttgctgaa
    12001 taaagtgcat catctgatgc gccgtgacga tgccatccca caggtggcgt taaccgccag
    12061 cgtgatggat gcgatgctgg aactcagccg caccggtctg gggctggtgg cggtatgtga
    12121 cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct ggctggtagg
    12181 cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca ccacgttaca
    12241 ggcgcaaagt cgcgccatcg acgccaaaga gatcctgatg aagcgcaaaa tcactgccgc
    12301 accggtggtg gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg atttctatca
    12361 ggccgggatt atttaatcct tcattcccag acgtttcgcc agccgatgca ggttggcgac
    12421 gtcggtttcc agcatccgcg cgcaggcagc ccagttatga tgattttgtg ccagtgcctg
    12481 gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct taacaacggg
    12541 caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc
    12601 aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc gatgaatagc
    12661 atgttccagt tcgcgcacgt tccccggaaa acgatagtgt tgcagtaaat ttcgcgctcc
    12721 ggcacttaat accacgcggg agagccccag ccgcaaacga cactgctcgc agaaataccc
    12781 cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa gtggaaacac
    12841 gctcaggcga tgaaacaagt cagcgcgaaa tcgccctgcc atcacctctt cgcgtaagtc
    12901 gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat cgccaacgcg
    12961 ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca acgacaactc
    13021 gccgatctca tccagaaaca gagtgccgtt atccgccatt tcaaacttcc cgctgcggtt
    13081 actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt ccgccacact
    13141 ttccggcagt gcagcacagt tgagatagac cagcggattc accgcccgtg gcgaggcttc
    13201 atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga tcaggacgtt
    13261 gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc caggggacaa
    13321 gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg gcatcatatt
    13381 ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg ccagcgcagc
    13441 aatcagccgt agctcttcgt cgctgaaaac atcgaactga tcgggctgca tcccgtcgag
    13501 cgtcagtgcg ccgatcaggt tttgcccggc aaacaatggc agaccaacgc aggcgtgaac
    13561 cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt cgctgtctgc
    13621 gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat gcccttccag
    13681 cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg gaataaactg
    13741 ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac gtagcgtggt
    13801 gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga tggcgatatt
    13861 cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac tatagatatt
    13921 gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatgagc ataattttat
    13981 ttatagagta aaaacaatca gataaaaaac tggcacgtaa tctgcaatta gcaagacatc
    14041 tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa aaataacatt
    14101 cattgggttg gtcaacgtga ctgggaagtg cgtgattttc acggtacgga atataaaacg
    14161 ctacgcggca gcagttacaa cagctacctc atccgcgaag aaaaaaacgt gctgatcgac
    14221 accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga aatcgatctg
    14281 gcggatatcg attacatcgt gattaaccat gcagaagagg accacgctgg ggcgctgacc
    14341 gaactgatgg cgcaaattcc cgatacgccg atttactgta ctgccaacgc tatcgactcg
    14401 ataaatggtc atcatcatca tccggagtgg aattttaatg tggtgaaaac tggtgacacg
    14461 ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa cgccaatgct gcactggccg
    14521 gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga tgctttcggt
    14581 cagcactact gcgacgagca tctgttcaac gatgaagtgg atcagacgga gcttttcgag
    14641 cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt aacaccgaaa
    14701 attaccgaga tcctgggctt taacttacca gtcgatatga tagcgacctc tcacggcgtg
    14761 gtatggcgcg ataacccgac gcaaattgtc gagctgtacc tgaaatgggc ggctgattat
    14821 caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg catgatggct
    14881 gacgctatcg cccaggggat tgcggaaacc gacccacgcg tggcggtgaa aattttcaac
    14941 gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa aggcgtgctg
    15001 gtcggcactt cgacgatgaa taacgtgatg atgccgaaaa tcgccggtct ggtggaggag
    15061 atgaccggat tacgcttccg taacaaacgc gccagtgctt tcggctctca cggctggagc
    15121 ggcggtgcgg tggatcgtct ttccacgcgc ctgcaggatg cgggtttcga aatgtcgctt
    15181 agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgccg tgaacacggt
    15241 cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt gaatacggtg
    15301 gttaaagaag aaacctctgc caccacgaag tccgacctcg gcccacggat gcagtgcagc
    15361 gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt tgcgccagga
    15421 acgccgtgga gtgaagttcc ggataacttc ctctgcccgg aatgctctct cggcaaagac
    15481 gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc attggttcgg
    15541 gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccagt attccattaa
    15601 ccctgatcgc cgccgacagc atggatgagt acaacaaacc ggacctcagc catgttatca
    15661 gccaggggca acgtgccgac gaccttaccc gccagacggc gggtgaattt gccgggcagt
    15721 ttaatctgca cctgtttccg cacacctggg tgacggatat cgatgccgaa gcccatgtgg
    15781 tgaaaagcca gaataatcag tggcaatacg acaagctggt gctggcaact ggtgccagtg
    15841 cctttgtccc gccagtgcct gggcgtgagt taatgctgac gttaaatagt cagcaagagt
    15901 atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc ggcggtggtt
    15961 tgattggtag cgaactggcg atggattttt gtcgtgcagg caaagcggtc acgctaatcg
    16021 acaacgctgc cagtattctg gcgtcattaa tgccaccaga agtaagcagc cgcttgcagc
    16081 atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag gggctggaaa
    16141 aaacggattc tggcattctg gcaacgctgg accaccagcg tagtatcgaa gtggatgcgg
    16201 taattgccgc caccggactg cgcccggaaa cagccctggc acgacgcgcc gggctgacga
    16261 ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgacgat atttatgcgc
    16321 tgggcgattg cgcggaaatc aacggtcagg tactgccatt cctgcagccg attcaactta
    16381 gcgcgatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa ctaccggcga
    16441 tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa acccagcgtc
    16501 aggatttacg ctggcaaatt aataccgaac gccagggaat ggtggctcgc ggtgttgacg
    16561 atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag gcatttggat
    16621 tgttgaaaac attgccgatg taggtgggct actgtgccta aaatgtcgga tgcgacgctg
    16681 gcgcgtctta tccgacctac ggggacgcat gtgtaggccg gataaggcgt ttacgccgca
    16741 tccggcaatg gtgtccaaat gcaacacgtt ttatccgttc tggacttcac ccgctaacca
    16801 acgcgccgca gcaataaccc cctgccccag agacaaaccg ccatcacccg ccggtaaact
    16861 ctgtggaaag agcaatgtga aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg
    16921 gttatgaata accccgccgc taaataccag cgtagtgata ccacgcatcg tggcctgctc
    16981 acgcatcaac gcggcaaaac cctgcgccag cgcatcatga aacgcccacg cgcgttgatt
    17041 aaccggtgcc tgccagttca gccactgctg ccagaaagcg gcgagatcca gttgattgtc
    17101 caccagcggc atcgtcaccg gatgcgtcac tccgtggcat gaggctgcga gcgcctccag
    17161 agcacaagcc gcttcacctt cataacttaa cgtggctggc gcacagccca gtgccgccgc
    17221 cactgcatcg aacaaccgcc cacacgatga cgccagcggc gcgttaattc cacgctcaat
    17281 ggcccgcgcc agcacgctcc agttttgctg ttgcacactt gctgtttcag ggtaattctg
    17341 ccactccggc acaaagcgca ggcactgcgc cagcaggttt cgccacggct gcttcgctgc
    17401 caaatcgcca cccggaagcg ccactgcagg caagccgccc aggtgctcac attcgcgata
    17461 gttcacccgc aggcactcgc cgccccacaa agcgccgttc tcccccatac cgataccgtc
    17521 gagcgtcaaa gcaatgacat caccgccatc caacggccac tgatgctctg ccagacacgc
    17581 cgctgcatgg gcatgatgat gcagtaccgt ttgcgtcggc agattcattt cacgcgccca
    17641 ctggctggag acatagcccg gatgcgcgtc atgcacaacg tattgcgggg taaaatcgta
    17701 gatgttttgc atcaggcgta acgcttcgcg ccactgcatc tggatgccat cgtcacttaa
    17761 atcgcccaga tgctgactca acaccgcttg ttcaccgcgc accaggcaga aggtattttt
    17821 cagatccgcg ccgagacaca gcacaggcgg aacattttta aagcccggag gcaaagccag
    17881 cgcatccggc acataccccc gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga
    17941 atcatccatc cgctgcacga tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg
    18001 caaatccgcc agcgcctgtt cgttgctgat agctggtggt ttaccgctca ggttgccgga
    18061 ggtcatcacc agcgggcatt gcagttcctg taacagcaaa tgctggagcg ggttcgcagg
    18121 caacattacc ccgacttcgt taaggtcagg ggcgatatca tcacaaagct caggaacgta
    18181 ttttttatcc accagcacaa tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc
    18241 tggtaaaccg tcagccactg gcaacatgac cgccagcggt ttcgccgggc gatgtttgcg
    18301 cgcccgaagt gtcgccaccg cgttactgtt acgtgcatcg caggcaagat gaaatccgcc
    18361 aatccctttg atggcgacaa tgttgcccat ttttaactgt gcgatagccg cctgtaatgc
    18421 cgcctcttgt tctgcatgtt caccatgact tacccattca agatgtgggc cacactccgg
    18481 gcaggccacc ggctgggcgt ggaagcgacg atcgagcggg tcacggtact ctttgtcaca
    18541 agccggacac aacggaaacg ccgccatcac ggtaaacggg cggtcgtaag gcatggcgcg
    18601 aataatggtg aaacgcgggc cgcagtgggt acagttgata aacggataac gataacgccg
    18661 ttcgcctggg gtattcattt cggcaaggca agcagggcaa gtagcggcat ccgggacaat
    18721 ttgcgtattc atggcacctc ccgcgctctg gctgatggtg aactcagtgg gcagttgtga
    18781 ccagatgtac ggctcacgct cgacgctatc aatacgcgcc agcggcgggc agtgctgatg
    18841 caattgaaca agtaacgttt ccgggtcttc ccgcagccgg acttctacgc catcgccgtc
    18901 attacagaca tcgccgtgaa gatttaattg ctgtgccagc tgccagacaa acggacgaaa
    18961 accgacgccc tgcactttgc cacgaatacg cagttggaca ccgcaagatg tgttttttgc
    19021 cattgagtta ttcccgccat catgaattgc gtaacccgtc ctgccggaca cgactgcgtc
    19081 gtatccggca gtctcaggtc ggcgataccg ccagctccgt attctacgaa tatttccggg
    19141 aattcctttg atgccagaac agttctgtaa gatttttaga acatcagcgc cgtacggcgg
    19201 cgtttttctg cactcagttg ttcaagttta ttacgatcga cacaaatcag cgcatgagtc
    19261 gggcaagccg ccatacacgc cgggccgtct tcacgatggt tgcacaggtc acatttattg
    19321 gcttcggctt tgtcagcccg tacattcagc cccgcgccgc tgttgcgaat caccggacgt
    19381 accaccactt ccatcgcgcc atacgggcaa gccacaacgc aggttttgca accaatgcaa
    19441 cgttcctgca tcacatgaac aaacccttta tcacggctga tagcaccatt cgggcagacg
    19501 ttagcgcacg gtgcatcttc acactgacgg caaactgtcg ccgtggaaat gttcacacct
    19561 ttaatgacat ggatacgcgg taaaaatgtt tccggggtca gcgatgcaca gtcctgattt
    19621 tcctgatgag aaaccacgca cgccacttca caggtacggc aaccaataca tttactcgcg
    19681 tcagcaatga tgaaacggtt catcaaattc tccagcaatg acagttaatg cgccgataca
    19741 ttcacaaatc atgccagttt taattcactg ttatttaagg aaattaattt ctgtaatgca
    19801 ggaaaaaaga tgtcatcgac actagtgacg atgacatgtg atgacaatgt ttatcgcgaa
    19861 agagcaatga gtgagtcgcg gcggatcagt tttccgctga aggttttcgg cggtgagaaa
    19921 tccccgccat cgagcataaa aatcagccgt ccaataattt cctgaatcat ctcagtcacc
    19981 ggaattttta cgctggagag cgccggaacg atataggggg caattgcgat atcatcgaat
    20041 ccgataactg acacctgctc tggcaccgct acgccgcgct cgtgcaacgc tttcatcgca
    20101 cctatcgcca tatcgtcgtt actggcaact aacgcgctaa atttagcccc acgttcgagc
    20161 aacgtttcta ccccttcggc cccgctggcg ggcgtccatt taccgttagc gataagtttt
    20221 tcattgagcg caataccatg ctgcgccagc gcgtctttat acccagcaag acgttcaatg
    20281 ctggtggggg aatccatcga gccggtaagg aaagcaatct cctgatgtcc ggcgtttatc
    20341 aactctgcca cggcattaaa gctggtctgt ttatgatcgc accagacgct atggctgctg
    20401 tttttgcgca ggcggcgatt aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca
    20461 tcgatctcat ccacgcttaa aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc
    20521 agcagatact gaatcgcctg gcgctcttct tcggcgctgt gtttaccatc tgccaatagc
    20581 aactgccgcc ctttctcttc cgccattcgc gcggcatgaa agagtaattc actaaaataa
    20641 atgccgtggt aaagcgtgtt ggtcactacc aaccccagcg tctgagtact cttcgccgac
    20701 agattgcgcg ccagcaagtt tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga
    20761 tctttagtct cctggctgac gtagccatta cctgaaagca cgcgggaaac ggtcgctttt
    20821 gaaaccccgg cgcgcttcgc cacttccagc atcgtcgtca tcattttcat ccctttacac
    20881 gcaatcaacg cagtgtactg caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa
    20941 aattcaggtg accggtttca caaatataaa aaatgaacaa ttaactctct tgcttattta
    21001 gtgacaacta ttcatgattt tgtgaaaccg gtttcctaat tccgtttcag catcggcatt
    21061 tttccgttac gtcgactgat aacaactaca tctaccctac tgataacagg ataaaatccg
    21121 atggccaaaa attatgcggc gctggcacgc tcggtgatag cggcactggg cggcgttgat
    21181 aacatctcgg cggtcacgca ctgtatgacg cgtttgcgcg ttgttatcaa agatgaccag
    21241 cttatcgaca gcccgacgtt aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac
    21301 aaccagtgtc aggtgattat cggcaatacc gtttcacaag cctttcagga agtcgtcagc
    21361 ctgctgccgg gagatatgca gcccgcacag cccgtgggta aacccaaact cacgctgcgt
    21421 cgcattggtg cggggatcct cgatgcgctg atcggcacca tgtcaccgct gatcccggcg
    21481 attatcggcg gatcgatggt caaactgctg gcgatgatcc tcgagatgag cggcgtgctg
    21541 acaaaaggat cgccgacctt aaccattctg aatgtgattg gtgacggtgc tttcttcttc
    21601 ctgccgctga tggtcgcggc atctgccgcc atcaaattta aaaccaatat gtcgctggcg
    21661 attgccatcg cgggtgtgct ggtacatccg agttttattg aactgatggc gaaagcggcc
    21721 cagggtgaac atgtcgaatt tgccctgatt ccggtcaccg cggtgaaata cacctacaca
    21781 gtgatcccgg cgctggtgat gacctggtgc ctgtcatata tcgaacgctg ggtggatcgc
    21841 attacaccgg cggtgaccaa aaacttcctc aagccaatgc tgattgtgtt gattgccgca
    21901 ccgctggcaa tcctgctgat tggcccgatt ggtatctgga tcggtagcgc catttcggcg
    21961 ctggtttaca ccattcatgg ttatctgggc tggctttcag tcgccattat gggcgcgctg
    22021 tggcctctgc tggtaatgac cgggatgcac cgcgtcttta cgccaaccat cattcagacc
    22081 attgccgaaa ccggcaaaga agggatggtc atgccgtcag agatcggcgc taacctgtcg
    22141 ctgggcggtt catcactggc ggtggcgtgg aaaacgaaaa acccggaact gcgccagacg
    22201 gcgctggctg cggcggcatc agccattatg gcggggattt ccgaaccggc gttatacggc
    22261 gtggcgatcc gcctgaaacg tccgcttatc gccagtctta tcagcggttt tatttgcggc
    22321 gcggttgccg gtatggcggg gcttgccagc cactcaatgg cagcgccagg gttatttacc
    22381 agcgtgcaat tcttcgatcc ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg
    22441 gcgctggcgg tggtgctgtc gtttatcctc acactgttgc tcggctttga ggatattcct
    22501 gttgaggaag cggctgccga ggcgcgaaag catcagagcg tacaaccgac cgtcgccaaa
    22561 gaagtaagtc ttaattgagg atgaaaatgt cagtatttcc agaaggtttt ttatggggcg
    22621 gcgcgcttgc cgccaaccag tctgaaggtg cgttccgtga aggtggcaaa gggctgacca
    22681 ctgtcgatat gatcccacac ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac
    22741 gttttcagtt gcgcgatgac gagttttatc ccagccatga ggcgacggat ttttatcatc
    22801 gttataaaga agatatcgcc ctgatggcag agatgggatt caaggttttc cgtacctcaa
    22861 ttgcctggag ccgtctcttt ccgcagggcg atgaaatcac gcccaatcag cagggcattg
    22921 ctttttatcg ttctgtcttt gaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt
    22981 tgtgccactt cgatgtgccg atgcatctgg tcaccgaata tggctcctgg cgtaaccgca
    23041 agctggtgga gtttttcagc cgctacgccc ggacctgctt tgaagcattt gatggtctgg
    23101 tgaaatactg gcttaccttc aatgaaatca acattatgtt gcatagcccg ttctccggcg
    23161 cgggtctggt gtttgaagaa ggtgaaaatc aggatcaggt gaaatatcag gccgcgcatc
    23221 accagctggt tgccagtgcg ctagccacca aaatcgccca tgaggttaac ccgcaaaatc
    23281 aggtgggctg tatgctggcg ggcggtaact tctaccctta cagttgcaag ccggaagatg
    23341 tctgggcggc gctggagaaa gaccgggaaa acctgttttt tatcgatgtg caggcgcggg
    23401 gcacgtatcc ggcttactct gcccgcgtat tccgcgaaaa aggggtaacc atcgacaaag
    23461 caccgggcga tgatgaaatc ctgaaaaaca ccgtcgattt tgtctctttc agctattacg
    23521 cctcgcgctg cgcctcggcg gagatgaacg ccaacaatag cagtgcggcg aacgtagtga
    23581 aatcgctgcg taacccgtat ctgcaagtga gcgactgggg ctggggtatt gatccactcg
    23641 gtctgcgtat caccatgaac atgatgtacg accgttatca gaagccgctg tttctggtgg
    23701 aaaacggcct cggcgcaaaa gatgaacttg ctgccaatgg cgagattaac gatgactatc
    23761 gcatcagcta tttacgcgaa catatccgcg caatgggcga agcgattgca gatggcattc
    23821 cgctgatggg ctacaccacc tggggctgta ttgatttagt ctccgcctct acgggtgaaa
    23881 tgagcaaacg ctacggtttt gtctacgtag accgtgacga cgcaggcaac ggcacgctga
    23941 cgcgcacacg taagaaatcg ttctggtggt ataaaaaagt gattgccagt aatggggaag
    24001 atttagagta ggtaacagtg ccggatgcgg cgtgaacgcc ttatccggcc tacatgtcgc
    24061 acgaaccagg aaggcccgat aagcgcagcg catcgggcaa tctggggttt ggcatcagtc
    24121 aaaatcaaag aaaccattcc ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg
    24181 atatcgtcag ggatcgttgt ccagacaaaa tctgactgac cagggattta gagccgattt
    24241 cgttcttcaa atccgcatag gtcagattat gttgatcaat cagcgtgcga agtaacgcca
    24301 cgccaacagg cattgccgca acggctttgt cgaactctgc aaactcttgg ttgttgtttt
    24361 catattctgc aatgcggctc gccagaaagt cgataagtgg attggtgtca tcttcttcaa
    24421 tcaaatattc gaccagttcc agtgcttcac gataatcatc ttctgagtca ctacctccca
    24481 gaaaggggac tgcattgacc agttctcttg ttgctttaac agcgcgtgca gcattagcag
    24541 tcattctttg ttctctctgt agtaacgtgt cagtttgtca tactcggcgt gattagttat
    24601 gtgcttcaca aagaaccgct tattaacaaa gtttatgtag gcaatcaccc ttaggttgtt
    24661 cccacctaca tccagaacgt accatttgtt gcgatattta aaattgtcga ggttgggaaa
    24721 cgccgttcgc atctcttctg gcgtagaaaa atccgtttcc ttaataaccc gatacaatgc
    24781 ctgaagagcc agagcgtcgt taggatattt acgcgcagac tcttcaaatg gcgctttaga
    24841 aatgatgtgc atttttaagt tttaacttcc ttatagtaag taaacagcca catctgcgag
    24901 ttctatcctt atgtgttcgg aaactatagc tcatcaaatc caagttcaca atatgtaaac
    24961 acttgagcgc gatataatta cccccaaaat cctcatagcc actctgcaaa aagtcagttc
    25021 tggttcaccc catcaagaac atcccccttt atcaagcaac gctgcgtctg cgccagatct
    25081 tccgtttgcg ggtcgcgccc ggcgatgggt ttgaggacga gtttacggtt gggggtataa
    25141 gtaaaacgcg tgagcgcggg atagcttaac ttgccaaacc agccgtgaac acgctgccat
    25201 ttggcgtctt ttacctgata cagactggtt atccagtaac cggaatcaaa ctgcatatcc
    25261 agcaattgcg tgtgaccatt gccttgcaaa tccagcaaat catctacgcc agttttgctt
    25321 gcggtgtaga atcccctggg ttcaaagaca cagggacggc cttcgcgcgt gaacgtcata
    25381 agaattaaat gtgcataagg agccagacca ttaccggtac ttggtaacca gataaccaga
    25441 tcctgaatgc cgttacgatc aagatcggcg cgatagagtt gccctttgcc cgctatgttt
    25501 gccagcatac ccacaggcac agaccaggat ttaccggcgc gatctttacc gttcaattgc
    25561 cattcactat catcatttgg catcttttca agcgtcacgc cagcaacgcc cacttttcta
    25621 aaatgccagg tttgctcggc atcataaggc atggtcagga gaggtttcag cgcccgcttt
    25681 tgcgcgtatt cgctgtcact cattggcaga tattcggtaa agctggtggc ctgtgcaggc
    25741 agcactaagc tcaataaaaa agcgcacatc cctgttctga tattcatttc gctgaattcc
    25801 ctgatggaga attattcttc ttccaccgct aactgcgcga agccgccatt cccttcccag
    25861 ccttccagtc gttgataaac ggtttctacc gcatctttaa tcggctgagt catcgggtag
    25921 taaaagccga cgatatccgg ctggatgccg aggaaaatca cttcgccaat gtcttctttc
    25981 aactggtcga taagatagtt cagcggcatg ttatgggtag tcatcataaa catctcggcg
    26041 atatcatccg ggtcgatgat gcggatttcg ccggggttta gccccatatc agtagcgtca
    26101 acaatcagta accgcgtagg gcgcagttca cggatggcga cgatgtcgtt ttccggtgca
    26161 ctaccgccat caatcaccac ccagttacct ttcggcgcgg cggcgcactt ttccgccagc
    26221 agcgggccag cgccatcatc gcccatcatg ctattgccaa cacagagtaa aacgtcagtc
    26281 acgcagtctc ctcaccatca aatagatggc gttttcctga tgaatatcat gcagcatact
    26341 taacagcatt gtactccatt ccttctgctg cgaagtttgc gctgcccggg ctttatcgaa
    26401 tgcgttggcc agcatcggca catggttaat gtcgatgacg atctcaccat atttcggcac
    26461 gccttccatt ttgcggcggg cttcactgcc cgcctccaga gtggcaatcc atgccagata
    26521 ttcatcccac gggcaggtga gcgccgcttc caggcaatcg ataaccccaa ggtggtgacc
    26581 aatcgccagg ctgtaataga ccacctgctg cgcctcggcg ggcgtggcat cgttctcatc
    26641 aataaattta cggctcagtt gactgaacac caccttttca ctcatcggat acgcgcctct
    26701 tcaacaacat gattcagatg gctgacaatc tcgttcagac gcggatcgtt ttccgcttcc
    26761 agccagcgtg caacctgttc ttcgccctgc cctaactgtg tcaggaagtc atcggcaatc
    26821 tgacgaccgt aacgataacc cgccaggcgg cgtgcttcgc gatccacttt cacacgcagc
    26881 ggctgcacca tatcgccatg caggatttcc gcctgttgtt catcctgctc acccgggcca
    26941 cgggcgtgaa ttttctgctc cagcaggccg agcgccattg caaagccgta cagcgtggcg
    27001 gcaggcgttg gcgggcaacc aggaatgtag acatctaccg ggacgatttt atccgtaccg
    27061 ccccacacgc agtagagatc gtggaaaata ccaccgctgt taccgcaggc accgtaggag
    27121 atacaaattt tcgggtccgg cgcggactgc cacgcacgca gcgcagggga tcgcattgca
    27181 cgggtgaccg cgccggtaaa cagtaaaata tccgcatgac gcggtgaagg aacgacttta
    27241 atgccgaagc gttctgcatc aaacagcggc gaaagcgtgg cgaaaatttc gatttcgcag
    27301 ccgttgcagc cgccgcagtc cacgcgataa acgtaggcag aacgtttgat ttttttcagt
    27361 aacgacgcct tcatgctggc gatggattca tccaccgtca tcgggaccgg aatgccgttg
    27421 gcgtcacggg ggcctaataa attgctcatc agatggcctc tttcatatgg cgagtcagtt
    27481 caatacggtc ggacggcacc aggcatttct ggcgcttaca ttccgggcaa gtctcaaagc
    27541 tttcgcggtg gttttccgcg cggctgtcgc cgttgtgctt aagcagcgca atggcgtagt
    27601 cgatctcttt ctggacggcg aaaggacgat tgcagacgcg gcagttgcac agcgcgaagc
    27661 gggactgttg caggaagtct tctttcttcc acactgccag ttcgtactct tgcgacagtt
    27721 tgatcgccgc cgtcgggcaa acttcttcgc agcgtccaca gaagatgcag cgcccgagat
    27781 taaactccca cgccagctca ccggtggcga ggtcagtttc aaccgttaag gcgtttgacg
    27841 ggcaggcatt gacgcaggcc gcacagccga tgcactgctg cgggttctgc tccggcttac
    27901 cacggaagtt tttatcaacc gcaatcggct ccagcggata agacgaggtc gccgtgccgg
    27961 ttttgatgac ttttttgata aaggtaaaca tggcgattcc ttatttcagc ggcgagtttt
    28021 tacgctcaat gctgtaacgc tcgagttctt tgtacggcac cactttgctc tttttcttac
    28081 gcacatcgac cacggtcatg cggtcggtac aggagtagca agggtccaag ctaccgataa
    28141 tcagcggcgc atcggaaacg gtgttaccgc gcagcatgta gcgcagggtc ggccagttcg
    28201 cgtaggtcgc ggcacggcag cgccagcggt acagcttctg gttgtcgccg gtcatgctcc
    28261 agtggatatc gtcgccgcgc ggcgcttcgg caaagcccag cgcgaaacgg tgcggaatgt
    28321 aggtaaagcc ttccaccatc agcggaccgc ccggcaggtt atccagaccg tagtcgatca
    28381 tgttcagcgc ggtataaact tcgttgatac gcactttcag acgggaaata acgtcgcagc
    28441 cctgctcgct gtggacttcc attggcagca ggccataacc gacaaacggg tgatcggcgc
    28501 gagtatcgcg ggcgtgaccg ctggcgcgaa ccatcgggcc aacgttactg aaatcgcggg
    28561 ccatttccgg gtccagacga ccaatgccga cggtgcgctg ttccatgttc ggcgtactca
    28621 gcagcacatc caccagctcc tgcacttcac gacgcatctg ttgcgccagc tggcgggtct
    28681 ggatcatgtc atctttcaac agatcgcgac gaatcccgcc gatcaggttc aggccgtagg
    28741 ttttacgcgc accggtgagg atctctgcca ttttcatgga ggtttcacgc acgcggaaga
    28801 actgcataaa gccggagtca aagccggtaa agtggcaggc gagaccgagg ttgagcagat
    28861 gcgagtgcag gcgttccacc tccagcagaa tggcgcggat catctgcgca cgttctggca
    28921 cctgaatacc catcgcgttt tctaccgacg tggtgtaggc ggtgctgtgg gcaaagccgc
    28981 agatcccgca cacacggtca gagaggaagg tcacttcgtt gtaacccata cgggtttccg
    29041 ccagtttttc catgccgcga tgaacgtaga acagacggta gtcggcgtcg ataatgtttt
    29101 cgccatcgac gaacagacgg aagtggcccg gttcgtcaga agtgacgtgc agcggaccaa
    29161 tcggcacgac gttgtttttc ttgtcgccca gttcgttgat gaactcgtag gtttcagcat
    29221 cggtggtcgg tgccggacgc tgacgataat ccatgctgtc tttacgcagc ggataaagtt
    29281 catccggcag caccagacgg cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    29341 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    29401 ccggtttgtt ggcatcaact tcgacgcgaa ccgttaccca gcacttagtc cccttctcca
    29461 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaaa
    29521 gcaccgacaa ccagcccccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    29581 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    29641 ccggaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    29701 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    29761 cccagtccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    29821 cagcgcgata accaggacgc cgacgaccag tttgacgatg gcaatcacca gcgccagcag
    29881 cagcccaccg gcggtgaagg tttccatctg tccccacggg ataaatacgc cgacgaacat
    29941 ctgcagcacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctgcc
    30001 gctgtattca gagagcgggc cttcctgcag ctcctgctcg gcttccgcca ggtcgaacgg
    30061 cagtttgccc atttcgataa aggtggcgaa cgcacaggca caaagcgcca gtaccagcgg
    30121 gatgctctgg ctcagcggcc agtgataaac ggtgtcggtg atgttgctga tgttggtgga
    30181 accggcaacc tgtgcggcaa cccacagacc aagcagcagc atcggttcga ccagcacgcc
    30241 aagcatcgct tcacggctcg cgccgatagc ggtaaacggg ctaccggtat ccagaccaga
    30301 aatggcaaag aagaaacgcg cgatggcaaa gagatacagt aaggtgatta aatcacccag
    30361 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    30421 gcccaccatc acatacggcg tcaggcggaa cacccagccg gaggcatccg ggccaacgct
    30481 ctggcggccc agcagtttga aaatgtcacg atactcctgc aacacacccg gcccacgacg
    30541 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    30601 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    30661 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    30721 ccttcgcact gccagcccgg caccagcgac accggattca gccatttgcg cagtttcagc
    30781 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    30841 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    30901 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    30961 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    31021 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    31081 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    31141 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    31201 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    31261 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    31321 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    31381 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    31441 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    31501 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    31561 agccccagca ggataatgcc gatattttcc agggtgtggt aagccagcag gcgctggatg
    31621 ttgtgttcca tcaacgcata cagaccaccg acgaacgcgg tgatcatgcc gagcaccagc
    31681 agcgcgatcc cccaccacag cggtgcatta ccgcccagca gtgacagggt taacatgccc
    31741 agcaggccaa ttttcatgac taccgtagaa aacaacgcgg cagctggcgc agaggcgtta
    31801 gcatgtgcct gcggcaccca gccgtgcagc ggaataatcc cggccagcag gccaaagcca
    31861 atcacaccga gcagccagat atcggaaccg agcggcagct gttgcatacg catatccagt
    31921 aggcacagat ccagcgtgcc ataacgctgc cacagcagcc agcaagcaat caccagcagc
    31981 agagtgccaa gacgccccag cgcaaaccac agtttgccct ctttgctgtt gctggtgagg
    32041 aacaccgcac acaaggccat gacttcggcc attaccacga acatgccgag gttgctggcg
    32101 atgacggcgc agacggcggc agccatcaac atattgatct gcaagccgtt gcacttcacc
    32161 tgcgcgtggc gatgccagtc aatgttgtag aggctgacaa acagaccgca cagaccgagc
    32221 gtaatcagcc aaatcgcgtt aagcggagag atttgcacat cgtagcttac cagcgacagc
    32281 gcaccgctca cgccaaccgc gccagtcagt acagtgaagc ccgcggctgc cgtatacaga
    32341 ctaccaaccg cgccgccaat tccagctatc cagccactta acgctttttg aaaagaaaag
    32401 agaaatgcca gaacagcggc ggcgacaaac catgccacgc cgctattgat cagggaaatt
    32461 gcgctcattt agcctctcca ctttgagcct gctgaaacaa ggtgagatcg ccaaagtccg
    32521 tgttaaaggt cagctcacgc ttacgtttgc tgacgcgggc gatatcggtg ttatccacca
    32581 gatgcagggc tttagtcggg cacatccgca cgcaggccgg accttgttca tcaaaactgc
    32641 aaaggtcaca tttgacggcg atcgcgcgaa tacccggcac ccagtcaagc agcgtactga
    32701 tacgcgccgg agcaggcggt gccggtggcg ctttcggggt attggcgttt gccggaatat
    32761 ccagcggacg gctgccggaa aattcaattg cgccaaacgg gcaggcgatg ccgcacagtt
    32821 tgcagcttac gcacagactt tcattcaact gcacggcccc atcaatgcgg gtgatggcgt
    32881 taaccgggca aaccaccgca cagggtgcat cttcacagtg gtgacagagc tgcggcgcag
    32941 attctttttc attcagcatc actctcaggc gcggcattga ttgcaggccg tgctggcgat
    33001 gcgtctctga acaggcggcc tcacaagtgt ggcagccgat acagagcgtg gagtcaacaa
    33061 ttacaaaacg attcaccagg cattcctcag gtgattgtca tttttgacga aaacatgccg
    33121 atgaaatgtc attttcgaca ctcatcgaca cgcccatccc caaacaggcg taacgcctgc
    33181 aaaacgggca aagcctcagc tcatgctgcc gggctttgtc cctttaccag ttggcttaaa
    33241 ttcaccggca cattgttttc aatagcggtg tataagctgt cgtaaacgcc agcgattttt
    33301 tcgagatagt gttccagaac ctgttcgcga tcgcggatgt tgaccttgcc atcaatcatg
    33361 ccgtcaatgc tcagttgcgc ctgcgcaatc cgctgtttgt cttcgccatc ttttgtttcg
    33421 acgtaatact cgatggtgtg gctgttaaag ccatccgcca gggtgacgta aatacgaaaa
    33481 tgttcaaaaa ggacgaaaca gagttctttg tccggcgcgc agctcatggc gtgatgcagg
    33541 cgcgctttcg ataacgtgat cccctgaacc agcgaattgc agtagatgtg ccactggtcc
    33601 tgtaggcgac gatgccgctg tgcgatgtaa tcggctttct cgcttatttc ccaaatagtc
    33661 attgtcaggt tacccgttta acagagatgc cagctttaag cattttctat gccaactttt
    33721 aatttattgt tattaaatag atttttaagc taaagatgaa tttcgtcgcc gtgtcgacgt
    33781 gtcatttcga catcatcgac attattcacc gcagggataa tcaacactgg cacaattatt
    33841 gcttgtagct ggcaatagtt aatgggaggc gatatgcacg aaataaccct ctgccaacgg
    33901 gcactggaat tgatcgaaca gcaggccgca aaacacggcg caaaacgcgt aactggggtc
    33961 tggctcaaaa ttggcgcatt ttcttgtgtc gaaaccagct ctcttgcctt ttgttttgat
    34021 ctggtttgcc gcggcagcgt ggcggaaggt tgtaaactgc acctcgaaga acaagaggcc
    34081 gaatgctggt gtgaaacatg ccaacagtat gtgacgctac tgacccagcg cgtccgccgc
    34141 tgtccacagt gtcatggtga catgctacag attgtggcag acgacggttt acagattcgg
    34201 cggatagaaa tagaccagga gtgagcgatg tgtacaacat gcggttgcgg tgaaggcaac
    34261 ctgtatatcg agggtgatga acataaccct cattccgcgt ttcgtagcgc gccatttgcc
    34321 ccggcggcac gcccgaagat gaaaatcacc ggcattaaag cgcctgaatt tacccccagc
    34381 cagactgaag aaggcgacct gcattacggt catggcgaag cgggcactca cgcgccgggc
    34441 atgagccagc gtcggatgct ggaagtcgaa attgacgtgc tggacaaaaa taaccgtctg
    34501 gctgaacgca accgcgcgcg ctttgctgcc cgcaagcaac tggtgctcaa cctggtttcc
    34561 agccctggtt ccggtaaaac caccctgctg acggaaaccc taatgcgcct gaaagacagc
    34621 gttccgtgcg cggttattga aggtgaccag caaaccgtga acgatgccgc acgcattcgc
    34681 gctaccggca caccagcgat tcaggtgaac accggtaaag gctgccatct tgacgcacag
    34741 atgattgccg acgccgcacc gcgtctgcca ctggacgata acggtattct gtttatcgaa
    34801 aacgttggca acctcgtatg cccggccagc ttcgatctcg gtgaaaaaca caaagtggcg
    34861 gtgctttccg ttaccgaagg tgaagacaaa ccgctgaaat atccgcatat gtttgccgcc
    34921 gcctcgctga tgctgctcaa caaagttgac ctgttgccgt atctcaactt tgacgttgag
    34981 aagtgcatcg cctgcgcccg cgaagtcaat ccagaaattg aaatcatcct tatttccgcc
    35041 accagcggcg aagggatgga ccagtggctg aactggctgg agacacagcg atgtgcatag
    35101 gcgttcccgg ccagatccgc accattgacg gcaaccaggc aaaagtcgac gtctgcggca
    35161 ttcagcgcga tgtcgattta acgttagtcg gcagctgcga tgaaaacggt cagccgcgcg
    35221 tgggccagtg ggtactggta cacgttggct ttgccatgag cgtaattaat gaagccgaag
    35281 cacgcgacac tctcgacgcc ttacaaaaca tgtttgacgt tgagccggat gtcggcgcgc
    35341 tgttgtatgg cgaggaaaaa taatgcgttt tgttgatgaa tatcgcgcgc cggaacaggt
    35401 gatgcagtta attgagcatc tgcgcgaacg tgcttcacat ctctcttaca ccgccgaacg
    35461 ccctctgcgg attatggaag tgtgcggtgg tcatacccac gccattttta aattcggcct
    35521 cgaccagtta ctgcctgaaa acgttgagtt tatccacggt ccgggttgcc cggtgtgcgt
    35581 actgccgatg ggtagaatcg acacctgcgt ggagattgcc agccatccgg aagtcatctt
    35641 ctgtaccttt ggcgacgcca tgcgcgtgcc ggggaaacag ggatcgctgt tgcaggcaaa
    35701 agcacgcggt gccgatgtgc gcatcgttta ctcgccgatg gatgcgttga aactggcgca
    35761 ggagaatcca acccgtaaag tcgtattctt cggcttaggt tttgaaacca ccatgccgac
    35821 caccgccatc accctgcaac aggcgaaagc ccgcgatgtg cagaattttt acttcttctg
    35881 ccagcatatt acgcttatcc cgacgctgcg cagtttgctg gaacagccgg ataacggtat
    35941 cgacgcgttc ctcgcgccgg gccacgttag tatggttatc ggcactgatg cctataattt
    36001 tatcgccagc gattttcatc gtccgctggt ggtcgctggt ttcgaacccc ttgatctact
    36061 gcaaggcgtg gtcatgctgg tggagcagaa aatagcggcc cacagcaagg tagagaatca
    36121 gtatcgtcgg gtggtaccgg atgccggtaa cctgctggcg caacaggcaa ttgctgatgt
    36181 gttctgtgtc aacggcgaca gcgaatggcg cggcttaggc gtgattgaat cttctggcgt
    36241 gcacctgacg ccggattatc aacgattcga tgccgaagca catttccgcc cagcaccgca
    36301 gcaggtctgc gatgacccgc gcgcgcgttg tggtgaggta ttaacgggca aatgtaagcc
    36361 gcatcaatgc ccgctgtttg gtaacacctg taatcctcaa accgcatttg gtgcactgat
    36421 ggtctcctcc gaaggagcgt gcgccgcgtg gtatcagtat cgtcagcagg agaatgaagc
    36481 gtgaataata tccaactcgc ccacggcagc ggcggccagg cgatgcagca attaatcaac
    36541 agcctgttta tggaagcctt tgccaacccg tggctggcag agcaggaaga ccaggcacgt
    36601 cttgatctgg cgcagctggt agcagaaggc gaccgtctgg cgttctccac cgacagttac
    36661 gtcattgacc cgctgttctt tcctggcggt aatatcggca agctggcgat ttgcggcaca
    36721 gccaatgacg ttgcggtcag tggcgctatt ccgcgctatc tctcctgtgg ctttatcctc
    36781 gaagaaggat tgccgatgga gacactgaaa gccgtagtga ccagcatggc agaaaccgcg
    36841 cgcgcggcag gcattgccat cgttactggc gatactaaag tggtgcagcg cggcgcggca
    36901 gataaactgt ttatcaacac cgctggcatg ggcgcaattc cggcgaatat tcactggggc
    36961 gcacagacgc taaccgcagg cgatgtatta ctggttagcg gcacgctcgg ggaccacggg
    37021 gcgactatcc ttaacctgcg tgagcagctg gggctggatg gcgagctggt cagcgactgc
    37081 gcggtactga cgccgcttat tcagacgctg cgtgacattc ccggcgtgaa agcgctgcgt
    37141 gatgccaccc gtggtggtgt gaacgcggta gttcatgagt tcgcggcagc ctgcggttgt
    37201 ggtattgaaa tttcagaagt ggcactgccg gttaaacctg ccgtgcgcgg cgtttgcgaa
    37261 ctgctgggac tggacgccct taactttgcc aacgaaggca aactggtgat cgccgttgaa
    37321 cgcaacgcgg cagagcaagt gctggcagcg ttacattccc atccactggg gaaagacgcg
    37381 gcgctaattg gtgaagtggt ggaacgtaaa ggtgttcgtc ttgccggtct gtatggtgtg
    37441 aaacgaaccc tcgatttacc acacgccgaa cctcttccgc gtatatgcta ataaaattct
    37501 aaatctccta tagttagtca atgacctttt gcaccgcttt gcggtgcttt cctggaagaa
    37561 caaaatgtca tatacaccga tgagtgatct cggacagcaa gggttgttcg acatcactcg
    37621 gacactattg cagcagcccg atctggcctc gctgtgtgag gctctttcgc aactggtaaa
    37681 gcgttctgcg ctcgccgaca acgcggctat tgtgttgtgg caagcgcaga ctcaacgtgc
    37741 gtcttattac gcatcgcgtg aaaaagacac cccattaaat atgaagacga aactgttctg
    37801 gcacacggtc cggtacgccg cattttgtcg cgccctgata cgctgcattg cagttacgaa
    37861 gaattttgtg aaacctggcc gcagctggtc gcaggtgggc tataccccaa atttggtcac
    37921 tattgcctga tgccactggc ggcggaaggg catatttttg gtggctgtga atttattcgt
    37981 tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc tgcaaacatt tacgcagatc
    38041 gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca acaatgtcga ctatgagttg
    38101 ttatgccggg agcgcgataa cttccgcatc ctggttgcca ttaccaacgc ggtgctttcc
    38161 cgcctggata tggacgaact ggtcagcgaa gtcgccaaag aaatccatta ctatttcgac
    38221 attgacgata tcagtatcgt cttacgcagc caccgtaaaa acaaactcaa catctactcc
    38281 actcactatc ttgataaaca acatcccgcc cacgaacaga gcgaagtcga tgaagccgga
    38341 accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc tgatcaatct ccacgagcgg
    38401 gacgatttag ccccctatga acgcatgttg ttcgacacct agggcaacca gattcaaacc
    38461 ttgtgcctgt taccgctgat gtctggcgac accatgctgg gcgtgctgaa actggcgcaa
    38521 tgcgaagaga aagtgtttac cactaccaat ctgaatttgc tgcgccagat tgccgaacgt
    38581 gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa tccatcgtct gaaagaacgg
    38641 ctggttgatg aaaacctcgc cctgaccgag cagctcaaca atgttgatag tgaatttggc
    38701 gagattattg gccgcagcga agccatgtac agcgtgctta aacaagttga aatggtggcg
    38761 caaagtgaca gtaccgtgct gatcctcggt gaaactggca cgggtaaaga gctgattgcc
    38821 cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca tggtcaaaat gaactgcgcg
    38881 gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc atgagcgtgg ggcttttacc
    38941 ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg ataaaagctc tctgttcctc
    39001 gacgaagtgg gcgatatgcc actggagtta cagccgaagt tgctgcgtgt attgcaggaa
    39061 caggagtttg aacgtctcgg cagcaacaaa atcattcaga cggacgtacg tttaattgcc
    39121 gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag agttccatag cgatctctat
    39181 taccgcctga acgtattccc gattcacctg ccgccactac gcgagcgtcc ggaagatatt
    39241 ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc gtctggggcg caatatcgac
    39301 agcattcctg ccgagacgct gcgcaccttg agcaacatgg agtggccggg taacgtacgc
    39361 gaactggaaa acgtcattga gcgcgcggta ttgctaacac gcggtaacgt gctgcagctg
    39421 tcattgccag atattgtttt accggaacct gaaacgccgc ctgccgcaac ggttgtcgcc
    39481 caggagggcg aagatgaata tcagttgatt gtgcgcgtgc tgaaagaaac caacggcgtg
    39541 gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga aacgcacgac cctgctgtca
    39601 cggatgaagc ggctgggaat tgataaatcg gcattgattt aactgcaaat tgccggacag
    39661 atctgcctgt ccggcatact attcacgagg ttttttcgga cgatattttt ccggcagttc
    39721 tggtaccgga cgcttgtcat cgatgagatg acgcacggtt aagatcggat gacgccacag
    39781 cattctcggc ccggcccaac gcataatctg tttcatctct tcacgctttg caggctggta
    39841 acagtgcacc ggacactgct tacaggctgg tttctcttcg ccgaacacac atttatccag
    39901 ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc ggctccgctg acgcctgcgg
    39961 gcatttcgct tgataaagat cgatcatttt tttaatcgtc agtttttcac gagagatacg
    40021 cttgccggac atgctgcctc catactctta aggtgtattt atattacaac ttaatttgaa
    40081 aggggactat ggctctgaag gaggaggttt attcaaccga cgcaattttg cgtacttgct
    40141 tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc cggaaccagc atcaagaact
    40201 cgccgttcgc ttcttcccct gaaatgatta actccggtat catgtgcgcc ttatgtgatt
    40261 acaacgaaaa taaaaaccat cacaccccat ttaatatcag ggaaccggac ataaccccat
    40321 gagtgcaata gaaaatttcg acgcccatac gcccatgatg cagcagtatc tcaggctgaa
    40381 agcccagcat cccgagatcc tgctgtttta ccggatgggt gatttttatg aactgtttta
    40441 tgacgacgca aaacgcgcgt cgcaactgct ggatatttca ctgaccaaac gcggtgcttc
    40501 ggcgggagag ccgatcccga tggcggggat tccctaccat gcggtggaaa actatctcgc
    40561 caaactggtg aatcagggag agtccgttgc catctgcgaa caaattggcg atccggcgac
    40621 cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt acgccaggca ccatcagcga
    40681 tgaagccctg ttacaggagc gtcaggacaa cctgctggcg gctatctggc aggacagcaa
    40741 aggtttcggc tacgcgacgc tggatatcag ctccgggcgt tttcgcctga gcgaaccggc
    40801 cgaccgcgaa acgatggcgg cagagctgca acgcactaat ccggcggaac tgctgtatgc
    40861 agaagatttc gccgagatgt cgctgattga aggccgtcgc ggcctgcgcc gtcgcccgct
    40921 gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat ctgcaatttg gcacccgcga
    40981 tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt tgtgctgccg gttgtctgtt
    41041 gcagtatgcg aaagataccc aacgcacgac cctgccgcat attcgttcta tcaccatgga
    41101 acgtgagcag gacagcatca ttatggatgc cgcgacacgt cgtaacctgg aaattactca
    41161 gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg ctcgactgca ccgtcacgcc
    41221 gatgggtagt cgtatgctga aacgctggct gcatatgcca gtgcgcgata cccgcgtgtt
    41281 gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc accgccgagt tgcagccggt
    41341 actgcgtcag gtcggcgacc tggaacgtat tctggcacgt ctggctttac gaactgctcg
    41401 cccacgcgat ctggcccgta tgcgccacgc tttccagcaa ctgccggagc tgcgtgcgca
    41461 gttagaaact gtcgatagtg caccggtaca ggcgctacgt gagaagatgg gcgagtttgc
    41521 cgagctgcgc gatctgctgg agcgagcaat catcgacaca ccgccggtgc tggtacgcga
    41581 cggtggtgtt atcgcatcgg gctataacga agagctggat gagtggcgcg cgctggctga
    41641 cggcgcgacc gattatctgg agcgtctgga agtccgcgag cgtgaacgta ccggcctgga
    41701 cacgctgaaa gttggcttta atgcggtgca cggctactac attcaaatca gccgtgggca
    41761 aagccatctg gcacccatca actacatgcg tcgccagacg ctgaaaaacg ccgagcgcta
    41821 catcattcca gagctaaaag agtacgaaga taaagttctc acctcaaaag gcaaagcact
    41881 ggcactggaa aaacagcttt atgaagagtt gttcgacctg ctgttgccgc atctggaagc
    41941 gttgcaacag agcgcgagcg cgctggcgga actcgacgtg ctggtgaacc tggcggaacg
    42001 ggcctatacc ctgaactaca cctgcccgac cttcattgat aaaccgggca ttcgcattac
    42061 cgaaggtcgc catccggtag ttgaacaagt actgaatgag ccatttatcg ctaacccgct
    42121 gaatctgtcg ccgcagcgcc gtatgttgat catcaccggt ccgaacatgg gcggtaaaag
    42181 tacctatatg cgccagaccg cactgattgc gctgatggcc tacatcggca gctatgtacc
    42241 ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt acccgcgtag gcgcggcaga
    42301 tgacctggct tccgggcgct caacctttat ggtggagatg actgaaaccg ccaatatttt
    42361 acataacgcc accgaataca gtctggtgtt aatggatgag atcgggcgtg gaacgtccac
    42421 ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat ctggcgaata agattaaggc
    42481 attgacgctg tttgccaccc actatttcga gctgacccag ctaccggaga aaatggaagg
    42541 cgtcgccaac gtacatctcg atgcgctgga gcacggcgac actatcgcct ttatgcacag
    42601 cgtgcaggac ggcgcggcga gcaaaagcta cggtctggcg gttgctgctc tggcgggcgt
    42661 accgaaagag gttattaagc gcgcacggca aaaactgcgt gagctggaaa gcatttcgcc
    42721 gaacgccgcc gcgacgcaag tggatggtac gcagatgtct ttgctgtcag tgccagaaga
    42781 aacttcgcct gcggtcgaag cactggaaaa tctcgacccg gattcactga ctccgcgtca
    42841 ggcgctggag tggatttatc gcttgaagag tctggtgtaa taacaattcc cgatagtctt
    42901 ttgctatcgg gaatattaac gacaactgac gaatcaaata aaaataccct gtataatagg
    42961 aaagcttatt ttacagggta aaaccatgcc atctacacgc tatcaaaaaa tcaatgccca
    43021 tcactatcgc catatatggg tcgttggtga tattcatggt gaatatcagt tattacaatc
    43081 ccgttaacat caactctctt tttaccctga aaccgactta cttatttcca caggcgataa
    43141 tattgatcgt gggccgaaga gtcttaacgt gctgcgcctg ctaaaccaac cctggtttac
    43201 gtcggttaaa ggcaaccacg aagcgatggc gttggatgca ttcgaaactg gcgatggaaa
    43261 tatgtggctt gccagcggtg gtgactggtt tttcgattta aatgattcag agcaacaaga
    43321 ggcaatagat ctgttgctga aattccatca ccttccacat attattgaaa tcattaatga
    43381 caacataaaa tatgccatc
//
LOCUS       DAZPBM010000017        42323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPBM010000017 DAZPBM010000000
VERSION     DAZPBM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.017"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1043
                     /locus_tag="ACHUZQ_000821"
     CDS             <1..1043
                     /locus_tag="ACHUZQ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1024596.1"
                     /translation="DKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVL
                     YALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYNLLNTRDMILNELHQH
                     VFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDGNKDSRINKNVIVLAG
                     NHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVLTSHHGIIRNEENECY
                     CLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRSNAVASTPVYENYFNN
                     TTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDK
                     KGVPGLSCFQPQ"
     gene            1305..2927
                     /locus_tag="ACHUZQ_000822"
     CDS             1305..2927
                     /locus_tag="ACHUZQ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1024597.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTINDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3117..3230
                     /gene="yhiY"
                     /locus_tag="ACHUZQ_000823"
     CDS             3117..3230
                     /gene="yhiY"
                     /locus_tag="ACHUZQ_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1024598.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            3293..4360
                     /locus_tag="ACHUZQ_000824"
     CDS             3293..4360
                     /locus_tag="ACHUZQ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1024599.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4357..7092
                     /gene="rbbA"
                     /locus_tag="ACHUZQ_000825"
     CDS             4357..7092
                     /gene="rbbA"
                     /locus_tag="ACHUZQ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1024600.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7092..8216
                     /locus_tag="ACHUZQ_000826"
     CDS             7092..8216
                     /locus_tag="ACHUZQ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1024601.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8289..8561
                     /locus_tag="ACHUZQ_000827"
     CDS             8289..8561
                     /locus_tag="ACHUZQ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1024602.1"
                     /translation="MRTKVQALRKKKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIK
                     EGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            8558..8917
                     /locus_tag="ACHUZQ_000828"
     CDS             8558..8917
                     /locus_tag="ACHUZQ_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1024603.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(9037..9438)
                     /gene="nikR"
                     /locus_tag="ACHUZQ_000829"
     CDS             complement(9037..9438)
                     /gene="nikR"
                     /locus_tag="ACHUZQ_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1024604.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(9444..10250)
                     /gene="nikE"
                     /locus_tag="ACHUZQ_000830"
     CDS             complement(9444..10250)
                     /gene="nikE"
                     /locus_tag="ACHUZQ_000830"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1024605.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(10247..11011)
                     /gene="nikD"
                     /locus_tag="ACHUZQ_000831"
     CDS             complement(10247..11011)
                     /gene="nikD"
                     /locus_tag="ACHUZQ_000831"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1024606.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(11011..11844)
                     /gene="nikC"
                     /locus_tag="ACHUZQ_000832"
     CDS             complement(11011..11844)
                     /gene="nikC"
                     /locus_tag="ACHUZQ_000832"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1024607.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11841..12785)
                     /gene="nikB"
                     /locus_tag="ACHUZQ_000833"
     CDS             complement(11841..12785)
                     /gene="nikB"
                     /locus_tag="ACHUZQ_000833"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1024608.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12785..14359)
                     /gene="nikA"
                     /locus_tag="ACHUZQ_000834"
     CDS             complement(12785..14359)
                     /gene="nikA"
                     /locus_tag="ACHUZQ_000834"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1024609.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(14470..15057)
                     /gene="acpT"
                     /locus_tag="ACHUZQ_000835"
     CDS             complement(14470..15057)
                     /gene="acpT"
                     /locus_tag="ACHUZQ_000835"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1024610.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(15112..16161)
                     /locus_tag="ACHUZQ_000836"
     CDS             complement(15112..16161)
                     /locus_tag="ACHUZQ_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1024611.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            16293..17510
                     /locus_tag="ACHUZQ_000837"
     CDS             16293..17510
                     /locus_tag="ACHUZQ_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1024612.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(17514..18071)
                     /gene="dcrB"
                     /locus_tag="ACHUZQ_000838"
     CDS             complement(17514..18071)
                     /gene="dcrB"
                     /locus_tag="ACHUZQ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1024613.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(18144..18809)
                     /locus_tag="ACHUZQ_000839"
     CDS             complement(18144..18809)
                     /locus_tag="ACHUZQ_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1024614.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            19030..19275
                     /gene="tusA"
                     /locus_tag="ACHUZQ_000840"
     CDS             19030..19275
                     /gene="tusA"
                     /locus_tag="ACHUZQ_000840"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1024615.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(19377..21575)
                     /gene="zntA"
                     /locus_tag="ACHUZQ_000841"
     CDS             complement(19377..21575)
                     /gene="zntA"
                     /locus_tag="ACHUZQ_000841"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1024616.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(21649..22275)
                     /locus_tag="ACHUZQ_000842"
     CDS             complement(21649..22275)
                     /locus_tag="ACHUZQ_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1024617.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            22416..22775
                     /locus_tag="ACHUZQ_000843"
     CDS             22416..22775
                     /locus_tag="ACHUZQ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1024618.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22778..23047)
                     /locus_tag="ACHUZQ_000844"
     CDS             complement(22778..23047)
                     /locus_tag="ACHUZQ_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1024619.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(23037..23633)
                     /gene="rsmD"
                     /locus_tag="ACHUZQ_000845"
     CDS             complement(23037..23633)
                     /gene="rsmD"
                     /locus_tag="ACHUZQ_000845"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1024620.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23783..25279
                     /gene="ftsY"
                     /locus_tag="ACHUZQ_000846"
     CDS             23783..25279
                     /gene="ftsY"
                     /locus_tag="ACHUZQ_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1024621.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            25282..25950
                     /gene="ftsE"
                     /locus_tag="ACHUZQ_000847"
     CDS             25282..25950
                     /gene="ftsE"
                     /locus_tag="ACHUZQ_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1024622.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            25943..27001
                     /gene="ftsX"
                     /locus_tag="ACHUZQ_000848"
     CDS             25943..27001
                     /gene="ftsX"
                     /locus_tag="ACHUZQ_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1024623.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            27246..28100
                     /gene="rpoH"
                     /locus_tag="ACHUZQ_000849"
     CDS             27246..28100
                     /gene="rpoH"
                     /locus_tag="ACHUZQ_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1024624.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            28371..29474
                     /gene="livJ"
                     /locus_tag="ACHUZQ_000850"
     CDS             28371..29474
                     /gene="livJ"
                     /locus_tag="ACHUZQ_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1024625.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(29673..30056)
                     /gene="panM"
                     /locus_tag="ACHUZQ_000851"
     CDS             complement(29673..30056)
                     /gene="panM"
                     /locus_tag="ACHUZQ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1024626.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            30480..31589
                     /gene="livK"
                     /locus_tag="ACHUZQ_000852"
     CDS             30480..31589
                     /gene="livK"
                     /locus_tag="ACHUZQ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1024627.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            31637..32563
                     /gene="livH"
                     /locus_tag="ACHUZQ_000853"
     CDS             31637..32563
                     /gene="livH"
                     /locus_tag="ACHUZQ_000853"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1024628.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            32560..33837
                     /gene="livM"
                     /locus_tag="ACHUZQ_000854"
     CDS             32560..33837
                     /gene="livM"
                     /locus_tag="ACHUZQ_000854"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1024629.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            33834..34601
                     /gene="livG"
                     /locus_tag="ACHUZQ_000855"
     CDS             33834..34601
                     /gene="livG"
                     /locus_tag="ACHUZQ_000855"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1024630.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            34603..35316
                     /gene="livF"
                     /locus_tag="ACHUZQ_000856"
     CDS             34603..35316
                     /gene="livF"
                     /locus_tag="ACHUZQ_000856"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1024631.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            35600..36664
                     /gene="ugpB"
                     /locus_tag="ACHUZQ_000857"
                     /pseudo
     CDS             35600..36664
                     /gene="ugpB"
                     /locus_tag="ACHUZQ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            36762..37649
                     /gene="ugpA"
                     /locus_tag="ACHUZQ_000858"
     CDS             36762..37649
                     /gene="ugpA"
                     /locus_tag="ACHUZQ_000858"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1024632.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            37646..38491
                     /gene="ugpE"
                     /locus_tag="ACHUZQ_000859"
     CDS             37646..38491
                     /gene="ugpE"
                     /locus_tag="ACHUZQ_000859"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1024633.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            38493..39563
                     /gene="ugpC"
                     /locus_tag="ACHUZQ_000860"
     CDS             38493..39563
                     /gene="ugpC"
                     /locus_tag="ACHUZQ_000860"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR1024634.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            39560..40303
                     /gene="ugpQ"
                     /locus_tag="ACHUZQ_000861"
     CDS             39560..40303
                     /gene="ugpQ"
                     /locus_tag="ACHUZQ_000861"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1024635.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(40290..40730)
                     /locus_tag="ACHUZQ_000862"
     CDS             complement(40290..40730)
                     /locus_tag="ACHUZQ_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1024636.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            40850..>42323
                     /gene="ggt"
                     /locus_tag="ACHUZQ_000863"
     CDS             40850..>42323
                     /gene="ggt"
                     /locus_tag="ACHUZQ_000863"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1024637.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        10045 a        10735 c        11660 g         9883 t
ORIGIN      
        1 aggataagtt tgatagctat gttaaatctg tgttttctaa aatagacttt tataaacaat
       61 gtggtgtgat taacccacaa aatgccaaca cggcttattt tggcgataca gatggaagag
      121 taggcgctgt actttatgca ttgctcgttt cagggcatat aggcatcagg gaaaagggtt
      181 ggagtttatt atgtgaatta ttaaaacacg aaaacatggc ttcatctgca tatatacata
      241 aaaataataa attgctctac aatttattaa atacgagaga catgatattg aatgagttgc
      301 atcaacatgt ttttttaaag gacgatgcga taaccccttg tatcttcctt ggggatcata
      361 caggcgatag atttagtact atttttggtg ataaatatat ccttactttg ctaaattcta
      421 tgcgaaatat ggatggtaat aaagatagcc gtattaataa aaatgtcatc gttctggctg
      481 gaaatcatga aatcaatttt aatggtaact atactgcacg actagaaaat tataaactct
      541 ctgccggtga tacgtataac ttaattaaaa cgctcgatgt ttgtcattat gactcagaaa
      601 cgaaagtttt aaccagtcat cacggaataa taagaaatga agaaaatgaa tgttattgtc
      661 tcggtgcttt gcaggttcca tttaatcaga tgaaagaccc aattgatcct gaggaattag
      721 caaatatatt taataaaaaa cataaacaac acatggatga tcgtcttatc catctaatta
      781 gaagcaatgc tgtagcatcc acacctgtat atgaaaatta ttttaataac actactgctt
      841 tcagaccaaa acctgaagat atcttcaaat gtggacagac gctcaaaaaa actaaacaga
      901 aatatggcca ttatggatta ggggtagatc agcatcaaaa aattgataat tataccatgg
      961 ggctaaattc ctggaagata gcacctaacg aacgtggtga taaaaaaggt gtcccagggc
     1021 ttagttgttt tcaacctcaa taaaacttca tgagtgctgt ggtatctctt tttaattttg
     1081 ccagtatttc ataggttttc ttaatcgatt gtagaggcat gttctgatga cgcctctaca
     1141 atctttaggg actttctctg ctatgagata agaaatttca tattatgctt taaggctgaa
     1201 tgatttgtgc aaaatataat tattttccca tcgaacatga ggtgtatgtc ataagattaa
     1261 atgttaacta tgtattcctc gcggtaaata atatgaggta aaagatgaaa attggaactg
     1321 tggcaggcac taacgacagt acaacgacaa tcacaacaaa tgatatggtg caagagcatg
     1381 ttaccaactt cactaaagaa ctgtttgggt atattgcaaa tggtataggt gatgatatat
     1441 caagtattgc caggacaatg ctcggtgagg ttgtggaaaa aatagatgat tggcaaatag
     1501 aacggttcca acaatctatt caagatgaca aaatttcatt taccatacaa acgaatcatt
     1561 cagaaaaata tagtatgcta tcgggcatgc gcgctcatat tctacgtagg aataactgtt
     1621 atcagtttat tgtgacaatt aatagtaaaa attacggttg ccctcttgat aataccgata
     1681 ttaattggtg cagcattgtt tatttactta ataatatgac aataaatgat aatgctaatg
     1741 atgttgcagt tactgagtcg tacaaaccca tctggaactg gaaaatatcg caatacaatg
     1801 tatttgatat taaattcgaa acaatgataa aaccccagtt cgctgacaga acctatttct
     1861 ccaattgttc acctgttgat cccacaagta ccagaccgac ctattttggc gataccgatg
     1921 gctcagtcgg tgcagttttg tacgcgcttt ttgcaacggg acatttggga atcatggctg
     1981 aaggagaaaa tttccttagc cagttgctta atatagaaga tgaagttctc aacgtattgc
     2041 taagggagaa ctttaatgag caacttgaca ctaatgttaa tataataatc agtatcctga
     2101 acagaagaga cattgtttta gaaagtcttc agccttattt ggttattaat aaagacgcag
     2161 tcacgccatg tacgtttctg ggcgaccaga ctggcgatcg atttagtaat atctgtggcg
     2221 ataaatttat catcgattta ttaaaacgca taatgagtat caatgaaaac gttcatgtat
     2281 tagctggaaa tcatgaaacg aattgcaatg gtaactatat gcagaacttc acgcgcatga
     2341 aaccacttga tgaggatacc tactctggga taaaagatta tcctgtatgt ttctatgacc
     2401 ccaaatacaa aataatggca aatcatcatg ggataacttt tgatgatcag cgaaaacgtt
     2461 acattatagg accgataaca gtctctagtg atgaaatgac caatgctctc gatccggtcg
     2521 aattagcggc aattatcaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
     2581 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
     2641 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
     2701 cacataacgg taatggtgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
     2761 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
     2821 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
     2881 atggacttat gcctttgatc agaaggcgca caatgttaca gctctgaacc atagacaaca
     2941 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
     3001 ttcctgcaac tgaacaggcg tgaaacgcta tccaacagga tggataccgt tttgtcagcc
     3061 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
     3121 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
     3181 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
     3241 gcggcagttg taacacgcgt cgtttgcttg cagggattat ggcgggtcga ttatggataa
     3301 gagtaagcgc catctggcgt ggtgggttgt cgggttactg gtggtggctg ccgtcgtggc
     3361 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     3421 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     3481 gaaagaaggc cagtttgttc gcgaaggcga agtgctggcg aagatggata ctcgcgtgtt
     3541 gcaggagcag cgactggaag ccatcgcgca aatcaaagaa gcacaaagtg ccgttgctgc
     3601 cgcgcaggct ttgctggagc aacggcaaag cgaaactcgt gccgcgcagt cgctggttaa
     3661 tcaacgccag tccgaactgg actccgtggc taaacgtcat acgcgttccc gctcgctcgc
     3721 ccaacgaggg gctatttctg cgcaacagct ggatgacgat cgcgccgccg ctgagagcgc
     3781 ccgagctgcg ctggaatcgg caaaagctca ggtatcggct tctaaagcgg ctatagaagc
     3841 ggcccgtacc aatatcattc aggcccaaac tcgcgtcgaa gccgcgcaag ccactgaacg
     3901 gcgtattgcc gcagatatcg atgacagtga actgaaagcc ccgcgtgacg gacgcgtgca
     3961 gtatcgggtt gctgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     4021 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctgaa
     4081 acttggcggt gaagcccggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     4141 catcagtttt gtcgccagtg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     4201 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     4261 tctggaatat gtcaaaaccg gtttaccggg cgtagcgtgg gtgcgggtga atgaagaact
     4321 tccgtggcct gacgacctcg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     4381 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaaa cgttgcgctg
     4441 aacaatatta ctctcgatat tccggcccgc tgtatggtcg ggctgattgg tccggatggc
     4501 gtcgggaagt cgagcttatt gtcgttgatt tccggtgccc gcgtcattga gcagggcaat
     4561 gtgatggtgc tgggcggcga tatgcgcgac ccgaggcatc gccgcgacgt ctgcccgcgc
     4621 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     4681 aacgtcgatt ttttcgctcg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     4741 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     4801 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     4861 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgctccca gttctgggat
     4921 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     4981 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     5041 actggcagcg ccgaagagct acggcagcaa acgcaaagcg ctacgctgga agaagcattt
     5101 ataaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     5161 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     5221 ttcgttgccg ttgatcacgt taatttccgc attccacgcg gggagatttt tggttttctt
     5281 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc tcaccggact gctgcccgcc
     5341 agcgaaggtg aggcgtggct gttcgggcaa ccggttgatc caaaagatat cgatacccgc
     5401 cgtcgggtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     5461 aaccttgagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcaagagtg
     5521 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     5581 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     5641 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     5701 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctctac ccactttatg
     5761 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     5821 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     5881 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     5941 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     6001 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     6061 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     6121 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     6181 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     6241 cgtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     6301 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     6361 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     6421 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgcta tcgctataac
     6481 ccggacgtaa aaagcctgcc agcgattgtt ccggcggtga tcccgcttct gctgatgatg
     6541 atcccgtcaa tgctaagcgc ccttagcgtg gtgcgggaaa aagagctagg gtcgattatc
     6601 aacctttacg tgacccccac cacccgtagc gaatttttgc ttggcaaaca gctgccgtac
     6661 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     6721 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     6781 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     6841 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     6901 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     6961 actatcgccc gcggaacgtt ctcgaaagcg ctggatctga ctgatttgtg gcaacttttt
     7021 atcccgttgc tgatagccat cccgctggtg atgggcttaa gcatcctgct gctgaaaaaa
     7081 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     7141 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     7201 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     7261 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     7321 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     7381 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     7441 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     7501 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     7561 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa cctcgatccc gcgtggtttg
     7621 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     7681 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     7741 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     7801 gattatcgct ggtgctgatg gcgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     7861 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     7921 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     7981 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     8041 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     8101 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     8161 cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     8221 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     8281 gacatgccat gagaaccaag gtacaggctt tacggaagaa aaaaaataca ttggatcaaa
     8341 tatttaaaac tcctgttcct caagggatca agtggtctga tatagagtca ctggttaaag
     8401 cattaggcgg agaaattaag gaaggaagag gttcgcgttg taagttcata ctaaatatga
     8461 gcgttgcgtg tttccatcgg cctcatccgt cgccagatac cgataaaggc gctgtagaaa
     8521 gcgtgcgtga ctggttacta agtatagggg taaaaccatg atcaaactga agacgccaaa
     8581 ctctatggag attgccgggc aaccagctgt tattacctac gtgccagaac tgaatgcatt
     8641 tcgtggcaaa ttcctgggct tgtcaggtta ttgtgatttt gtctctgaca gcattcaggg
     8701 gctgcaaaaa gaaggggaac tttccctgcg agagtatctt gaggattgta aagctgcggg
     8761 tattgagccg tatgcgcgta cagaaaagat taagaccttt acgttacgct atccagaatc
     8821 gctgagtgaa cgccttaata atgcggctgc gcagcaacaa gtctctgtta atacctatat
     8881 tattgaaaca ctgaatgagc gtttgaatca tttataaatt ttagcggcgt acattacgca
     8941 gaatatctgc ctttacagga tctatctatg caactgaact taattttata aattaagttc
     9001 agttaatatc ttttctttcc tcgtttcaga aataactcaa tcttccttcg gcaagcactg
     9061 caaatgccca tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc
     9121 gcccatatca cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg
     9181 cagcgtggcg acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa
     9241 gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg
     9301 ctgagtggcc tcctgggcca gggcgctacg cagaatatcg cggatagctt cggaacggtt
     9361 gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt
     9421 gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat
     9481 gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct
     9541 cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag
     9601 cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt
     9661 agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct
     9721 tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg
     9781 agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc
     9841 gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg
     9901 cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag
     9961 tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc
    10021 gccagcggtt cgccacgcca gctaatattc ccctgcgcgg gcgattctaa acccaccagc
    10081 aaccgcgcga gggtactttt cccgcagccg ctgcgcccca gcagggcgac ggtttcgccg
    10141 cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt tccgttaaat
    10201 ccaccgtggg catagtgatg ggaaaggccg gagacgttaa gtaaagtcat gatgccagct
    10261 ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgttaccgca tgtttggggg
    10321 cgttaaacag cgtttctaca tcgccctgtt cgacaatttt accttgtgac atcaccgcca
    10381 cgtcatccgc cagacgcgcc acaacgccca tatcatgggt gaccagcagc attcccggcg
    10441 cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga
    10501 ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa
    10561 tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg
    10621 cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt
    10681 tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgtagc ggattaaagg
    10741 cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg cagggataaa
    10801 ccgctttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc
    10861 ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca
    10921 cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg
    10981 cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg
    11041 atccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag
    11101 cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt
    11161 aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc
    11221 cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat
    11281 caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga
    11341 cgccagcaca aactcgcgtt ggcgtagtga aatcaccaag ctgcgcacca tgcgtgcata
    11401 ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac
    11461 gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg
    11521 catggtggcc tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc
    11581 taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgc
    11641 catcagccgc gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc
    11701 cggcgaaagc aggcgcgacg gcaaatcaat cgcctggggg tcatacggca gccaccactg
    11761 gctggtgagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca
    11821 gcgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc
    11881 agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc
    11941 agcgtaaagc actgaatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc
    12001 ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccaat cagttcgccg
    12061 atgtgcatcc ccacggcggt gatcatcggc agcgaggcat tgcgcagaat atggcggcgt
    12121 tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccaag tgacgtgacg ctgaccggcg
    12181 acgtccagca tactggcgcg cagcagtcgc gcgttaatcg ccagcgacat aaaggcaatg
    12241 gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc
    12301 agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg
    12361 ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac ggtcgcgatg gcgcgccgcc
    12421 cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc
    12481 agcgtggcgg gcaggaagtt cagcatatcg tccagcaccg ggcgttggct ggcgaatgag
    12541 ataccaaagt caagatgcag cgccttccac aaccaggtac cgtactggac gtacagcggc
    12601 tgatccagcc cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta
    12661 gataaacgca gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg
    12721 atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc
    12781 aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt
    12841 aggggatatt acccagctcc ggttttgata ccaccatcat cgagatgtag ctgataggca
    12901 gataaaccgc ctcgtcatgc agacgggtca aaatgtcgcg atacagcgcc tgacgttgcg
    12961 tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggc ttgtcggcta
    13021 atccttgctg tgcctggaag tcagcgtgcg acggtacgcg cattgaactc aggaaggcgt
    13081 gtggatcgta tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct
    13141 gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc
    13201 gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg
    13261 acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg
    13321 tccaaccggc tttttccagc aactctttcg ctttctgcgg atcgtactgg cgcggtttca
    13381 ggccgagatt agcgtaaggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc
    13441 catataacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta
    13501 ctgccagctc gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg
    13561 gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg
    13621 gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc
    13681 gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc
    13741 agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc
    13801 acggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg
    13861 agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg
    13921 cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt
    13981 ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg
    14041 ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga
    14101 aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg
    14161 aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt
    14221 taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtagtgattt
    14281 catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc
    14341 ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat
    14401 gacgatttta agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg
    14461 gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg
    14521 ggtgggggtg cagatcgcaa ggcttaaatt ttctagctga caatggctga ctgacagcga
    14581 ggagtgatag gtgctatcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac
    14641 gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac
    14701 ggcgtccatt tcagcgtgtt ccccgaggct gaacacggcg ttcgccagcc agcgccagtt
    14761 ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcgc tcaacagcag
    14821 agcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcgtttccg gcgcgaatgc
    14881 aggtttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa
    14941 tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc
    15001 cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc
    15061 agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg
    15121 taaccgactt ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt
    15181 ggtttccatc cagattttac acacactggt taatggcacc gaaagtagca tccctaccgg
    15241 gccgagcagc catccccaaa ttaacaatga aagaaatacc accatggtgg acatccccag
    15301 gcgatggccc atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa
    15361 caatgcgccg accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg
    15421 cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac
    15481 tgcccacatc agcgcaaact gcacccccat cagctccagc cccagccaga cgatgacacc
    15541 tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg
    15601 gtgtaatcct gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac
    15661 gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat
    15721 tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat
    15781 ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa
    15841 tttaaaaagt ttgcgcgtca gctccttatt aaacttcggc agcatagaga taaattcgtt
    15901 aaacgatgcc gccagtacgc cgaccagcgc ggttagtgcg atcagcatca ccaccactac
    15961 aatcgtaatg gcaacggggc gttgtactcc ccgacgaata aaccaggtaa cgagcgggtt
    16021 gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg
    16081 aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt
    16141 atcgggttga gggatttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg
    16201 agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa
    16261 tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc
    16321 taaacggatt gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct
    16381 acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct
    16441 ttagcgcttt ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc
    16501 gtcctcatgc cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt
    16561 tatgcggctg ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc
    16621 ccgtcatcag cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt
    16681 ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc
    16741 gagtgatttc gtggaacggc attgtcaatt acggggcgat ggcgatgggt gcgccgttag
    16801 gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg
    16861 cgctggtggc cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac
    16921 cgctgccgtt tcgcgcggtg cttgggcgcg tctggctgta cggtatggca ctggcactgg
    16981 cttctgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt
    17041 gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt
    17101 tattccctaa cggcattaac cgtatcggcg gcttaaacgt ggcgatgatt tgctttagcg
    17161 ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaagtcg
    17221 gcgtcttact ggcgggggca gggttttcgc tggtgttccc ggcattgggc gtagtggcgg
    17281 taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg
    17341 atttatcgct tggcgtgacc ggaccactgg ctgggctggt gatgagctgg gcgggcgtac
    17401 cggtgattta tctggcggcg gcgggactgg tcgcaatcgc gttattactg acgtggcgat
    17461 taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga
    17521 ataaccagcg tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagct
    17581 ggcagagtaa tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag
    17641 tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc
    17701 agctcaatgg ctttgttggt taccacttgc agctgcggat cgcggctacg ttgctgatct
    17761 tccagacgct tcgccaacac cgccagatct tctttcggat catcgcccat gatgacgatg
    17821 actgctttct gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc
    17881 ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg
    17941 ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg
    18001 tctttatcat cacaggccgc aagccccata accagcaggc caattccgac atatttaacc
    18061 agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt catccatctt
    18121 atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct
    18181 gccagtcttt tcaacagaat attgagtaat acgccataca ttggcaggaa gaaaacgata
    18241 ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata
    18301 aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta
    18361 ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactctgacg caggcggtta
    18421 aaaacgtgca cgtcgaggat ctgtcccagc gcgtaggcca tgaaactggc ggtggcgata
    18481 cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccagga acccatatag
    18541 aatagcgacg agatgacgta ggagatcagc aacgccggga tcatcaccgc aaagatgata
    18601 cgacgggcca gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag gaaaataaac
    18661 gggaagctaa acgcgcccca ggtggtatgg aaacccaaaa tggagacggg aagctgaacc
    18721 agatagttac tggaggtgat caccagcaga tgaaataacg ataaccagaa caacgcctta
    18781 tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga
    18841 gggaacccaa tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt
    18901 tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc
    18961 gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat
    19021 gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg
    19081 cgctgcccgg aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcctggcgaa
    19141 acgttgctga ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc
    19201 tttatggaac acgaactggt tgcgaaagag acggatggac tgccttatcg ttatttgatt
    19261 cgtaaaggcg gttgatgggg aaagattggc ttcgatgccg ccttttcccc tcaccctaac
    19321 cctctcccca gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat
    19381 ctcctgcgca acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc
    19441 agcaccgcca gccacaggcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc
    19501 agccccagcg caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca
    19561 atcatttgca ccaggccgcg caggtggtta tgggttaatg ctgcatcagc ggtttccagc
    19621 gccacgtctg tgccactgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg
    19681 tcgttaatac catcaccgac catcgccagg ggcgcatgtt gattcagctt ggtcaccgct
    19741 ttgactttat cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt
    19801 gccgccgctg cacgtggatt atcgccggtg aggatcactc ctttgacccc cagcgcgttc
    19861 agttcactga tggcagttgc agcatcggcg cgcagggtat cctgcaatgc aatgataccc
    19921 agcacgtcat cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta
    19981 atcaaaccag caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca
    20041 ccgttaacct gcgcttcaat gccagacccg accagcgccc gctgtgattc ggcggtggga
    20101 atggcgagtt cagcaacctg tgcttcgcgt acgatggctt gcgccagtgg atgcgtcgcg
    20161 ccttgctcga ccgccgccgc cagtgtcagc agttcagatt cactaatacc cgttgccgga
    20221 tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc
    20281 tgagtaacac gacctagttg ttccagcgcc gctccgcctt taatcaacgc cccacgacgc
    20341 gctgccgccg ccagcccgga ggtaatcgcc gcaggcgttg agataactaa cgcacacggg
    20401 cagccaatca gcagcagcgt cagcccttta taaatccact cctgccagct ggcggcaaac
    20461 agcagcggtg gcaccagcgt caccagcaga gcgacggcca taatcgcggg cgtatagata
    20521 cggctgaaac ggtcgataaa ccgctcaatg ggggcgcgac gctcttcggc ttcttcgatc
    20581 agtttcagaa tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc
    20641 agacggtcta cgctggtggc tcctgcagga actttatcgc ccgtcgcgcg ctccaccgga
    20701 atggattctc cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg
    20761 tcggcaggca aacgcccacc tgcggcgact tcaatcacat cgccaggccg caggctgtta
    20821 atcgccacct cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc
    20881 attaacgcgc taacgccctg acgcgcgcgg ctggcggccc agccttccag tcgttcacca
    20941 atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa cagtgcacca
    21001 atagcggcta cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcagccgt
    21061 aatgcctgac gagcaatcgg gtacagccca accagcgtgg tcgcgataaa cgccagttgc
    21121 ccgaacggat gattgaactg ctccagaccc cagctgattg ccatcatcac gattagtgta
    21181 atcagcggca gattctcttt caggcgcgat gcttgtggct cgtcggcggc ctgctcatcg
    21241 cgcagggaat agcccgcttt ttgcaccgca gattcaactt gtgcacgaat gtcattgtcg
    21301 gcatcaacca ccagtttttc ggtggcgaac aacacctgca cctgattcac acctgcaagc
    21361 tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc actgactttc
    21421 cagctatagc gggtgccgga gacgttttca gagagcgttg gcgagctgga gcatgcgccg
    21481 tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga
    21541 ggggctttct tgccgtgatt gtcaggtgtc gacatggcat actccggtta agttttttct
    21601 cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag
    21661 tgagcggatg atgaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc
    21721 acggaagcgt cggcgatagt ggctccccag ccagacaaag ttactgataa acagcaacga
    21781 agcgccaaca aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc
    21841 cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc
    21901 cagccgcgtc cagataatcg ccagtaacag cgcacccaga accagcaaca ccagtggcag
    21961 aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga
    22021 gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc
    22081 gcccagcagt gaggcgcaca gccctgccag caccagatag ctaatggcgt cgaacatcgg
    22141 cgcttgccag gccagtaaca gcaggagaag aagggttaac ggtttaaaca cccagcgttg
    22201 ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat
    22261 aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac
    22321 ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt
    22381 atgcttattt ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta
    22441 ttgttatcat tggcttaatt gtcgtcgccg catcgtttcg ttttatgcaa cagcgacggg
    22501 aaaaagctga taatgatatg gctccgctcc agcaaaagct ggtggtggtg agcaacaagc
    22561 gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca
    22621 gtatgcgcta cgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc
    22681 tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag
    22741 gaacgcgctt tgttagcttt gttggcgaac aataaggtta ttttttaact ttaaatttct
    22801 tctgccagac cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg
    22861 tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca
    22921 tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt tagcgggcgc gggaagggat
    22981 gcaccagatt gaggattaaa aatccccaaa cgcagagcat taacaaacga ccaatattaa
    23041 tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc
    23101 actttttccc gatgtaacga ccagtttgcc ggaacggtgg gcagaccgtt ttcgacttcg
    23161 ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc
    23221 gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgtg
    23281 cctttttgcg ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt
    23341 agtgtcgcca gattcttaat taactgctga gaaactgcac gatccatctc aattaacgtg
    23401 gcccccgcag cgtagcgcga taacgcttcc agccccagcg cgccgctccc ggcgaagcaa
    23461 tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg
    23521 cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac
    23581 tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat
    23641 tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata
    23701 gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca
    23761 acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct
    23821 gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc
    23881 ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttcggaac aagccgttga
    23941 agagcagccg caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga
    24001 agtcactgaa caggttgttg aaagtgaaaa agcgcagcct gaagcggaag ttgttgcaca
    24061 gccggaaccg gtcgtagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc
    24121 gttgccggaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga
    24181 aaccgtagag attgtcaaag cggcggaaga agaagcggct aaagaagaaa tcaccgacga
    24241 agagctggaa gcacaggcgc tggcagccga agcagcagaa gaggcggtga tggtggttcc
    24301 tccggcagaa gaagagcagc cggtggaaga aatcgctcag gagcaggaaa aaccaaccaa
    24361 agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc
    24421 cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga
    24481 agagcagctt ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct
    24541 gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa
    24601 agaagagatg ggtgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac
    24661 gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaaccacga cgattggtaa
    24721 gctggcacgt cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt
    24781 ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat
    24841 tgcccagcat accggggcgg attccgcctc tgttatcttc gacgccattc aggcagctaa
    24901 agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca
    24961 cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc
    25021 gcatgaagtt atgctgacta ttgatgccag caccgggcag aacgcggtaa gccaggccaa
    25081 actgttccac gaagctgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc
    25141 gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg
    25201 tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact
    25261 ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt
    25321 gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg
    25381 accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg
    25441 cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa
    25501 gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac
    25561 cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat
    25621 attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac
    25681 ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg
    25741 aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg
    25801 gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca
    25861 acgcacgaca tcaacctgat ttcacggcgt tcccatcgca tgctcaccct gagcgatggt
    25921 cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt
    25981 tggtgggcgt ctggatcgct tccgtaaatc ggtcggcggc tcaggcgacg gcggtcgtaa
    26041 cgcaccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgcaaaacca acgttttcaa
    26101 cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc
    26161 cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat
    26221 ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt
    26281 ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc
    26341 cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg
    26401 taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt
    26461 ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtga
    26521 tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg
    26581 tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt
    26641 ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga
    26701 ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct
    26761 gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat
    26821 tctggtgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt
    26881 tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat
    26941 tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata
    27001 ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt
    27061 tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt
    27121 gcattgaact tgtggataaa atcactgtct gataaaacag tggatgataa cctcgttgct
    27181 cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga
    27241 tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct
    27301 acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg
    27361 aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc
    27421 ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga
    27481 ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg
    27541 tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc
    27601 gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc
    27661 tgcgtaaaac caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc
    27721 gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg
    27781 acatgacctt tgacctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc
    27841 tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag
    27901 agcaggcggc aaaccgtctg accgacgcga tgcaaggtct ggacgaacgc agccaggaca
    27961 tcatccgcgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc
    28021 gttacggtgt ttccgctgaa cgtgtacgcc agctggaaaa gaacgcgatg aaaaaactgc
    28081 gcgctgctat agaagcgtaa tttccgctat taagcagaga accctggatg cgagtccggg
    28141 gtttttgttt tttgggcctc tacaataatc aatttcccct ccggcaaaac gccaatcccc
    28201 acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa tcgggtatgt
    28261 tttagcagag tatgctgcta aagcacgggt agccatgcat aaaacgaaat aaagtgctga
    28321 acaacaacat cacaacacac gtaataacca gaagaatggg gattctcagg atgaacataa
    28381 agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag
    28441 aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc
    28501 aagagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag
    28561 gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg
    28621 tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat
    28681 caacgcagcc tgcgtctgac atctacgaag acgaaggcat tttaatgatc accccagcgg
    28741 caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg
    28801 actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta
    28861 ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg
    28921 gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag
    28981 atttctctac gctggtggcg cgtctgaaga aagagaatat cgacttcgtt tactacggcg
    29041 gttaccaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc
    29101 agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag
    29161 cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggca aacaaaccca
    29221 ttgttgacgc gatcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct
    29281 acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgaaatcg
    29341 ccaaatacct gaaagcgaac tccgtggata ccgtaatggg accgctgacg tgggatgaga
    29401 aaggcgatct gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcacggcga
    29461 cagatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag
    29521 tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctataag atccaaagaa
    29581 atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc
    29641 gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc
    29701 gccgtaaatc ccagtgcctg cataaacgct gtcatcacac cgcgatcttc cacgcctgca
    29761 tccgctatcc accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat
    29821 tgccccacac cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg
    29881 gtgccgctta aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag
    29941 atacggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc
    30001 aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt
    30061 aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc
    30121 tcttttttat ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa
    30181 acattagaga atgaaaaata cccagcataa tcccctgaat ggtagtgaat tattccgccc
    30241 tttgtgccgt tattttatgc tgacaaaggc acttttttct gtttatctat caataaattc
    30301 agaatattat ctgttcttaa tcgactgaaa aatagggatt ttaatcactg ttatcacaaa
    30361 atactgcgct aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt
    30421 ctgatttgtt aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta
    30481 tgaaacggaa tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg
    30541 ctatggctga cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg attgcccagt
    30601 ggggcgatat ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg
    30661 gaattaaggg cgacaagctg gttggcgtgg aatatgacga cgcctgcgac ccgaaacaag
    30721 ccgttgcggt cgccaacaaa atcgttaatg acggcattaa atacgttatt ggtcatctgt
    30781 gttcttcttc tacccaacct gcatcagata tctacgaaga cgaaggtatt ctgatgatct
    30841 cgccgggagc gaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg
    30901 ccgggctgga ctcttcccag gggccaacgg cggcaaaata cattcttgag acggtgaagc
    30961 cccagcgcat cgccatcatt cacgacaaac aacagtatgg cgaagggctg gcgcgttcgg
    31021 tgcaggacgg gctgaaagcg gctaacgcca acgtcgtctt cttcgacggt attaccgccg
    31081 gggagaaaga tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt
    31141 actacggcgg ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc
    31201 tgaaaactca gtttatgggg ccggaaggtg tgggtaacgc atcattgtcg aatattgctg
    31261 gcgatgctgc cgaaggcatg ttggtcacta tgccaaaacg ctatgaccag gatccggcaa
    31321 accagggcat cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct
    31381 ggatcaccta cgcggcggtg caatctctgg cgactgccct tgagcgtact ggcagcgatg
    31441 agccgctggc gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc
    31501 tgaactggga tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg
    31561 ccgatggttc atccacggca gccaagtgat catcccaccg cccgtgaaat gcgggcgggt
    31621 ttagaaaggt taccttatgt ctgagcagtt tttatatttc ttgcagcaga tgtttaacgg
    31681 cgtcacgctg ggcagtacct acgcgctgat agccatcggc tacaccatgg tttacggcat
    31741 tatcggcatg atcaacttcg cccacggcga ggtttacatg attggtagct atgtctcctt
    31801 tatgatcatc gccgcactga tgatgatggg cattgatacc ggctggctgc tggtggcggc
    31861 gggattcgtc ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtagc
    31921 ctatcgcccg gtgcgtaact ctaagcgcct gattgcgctc atctcggcaa tcggcatgtc
    31981 catcttcctg caaaactacg ttagcctgac cgaaggttcg cgtgacgtgg cgctgcccag
    32041 tctgtttaac ggtcagtggg tggtggggca tagcgaaaac ttctctgcct ctattaccac
    32101 catgcaggca gtgatctgga ttgttacttt cctcgccatg ctggcgctga cgattttcat
    32161 tcgttattcc cgtatggggc gcgcctgccg tgcctgcgcg gaagatctga aaatggcgag
    32221 cctgcttggc atcaacaccg accgggtgat tgctttgacc tttgttattg gcgcggcgat
    32281 ggcggcagtg gctggcgtac tgctcggtca gttctacggc gtcattaacc cctatatcgg
    32341 ctttatggcc gggatgaaag cctttaccgc tgcggtgctc ggtgggattg gcagcattcc
    32401 aggggcgatg attggcgggc tgatcctggg gattgcggag gcgctctctt ctgcctatct
    32461 gagtacggaa tataaagatg tggtctcatt cgccctgctg attctggtgc tgctggtgat
    32521 gccgaccggt attctgggtc gcccggaggt agagaaagta tgaaaccgat gcatattgca
    32581 atggcactgc tctctgccgc gatgttcttt gtcctcgctg gcgtctttat gggcgtgcaa
    32641 ctggagctgg atggcaccaa actggtggtc gacagcgctt cggatgtccg ttggcagtgg
    32701 gtgtttatcg gcacggcggt ggtctttttc ttccagcttt tgcgacctgc tttccagaaa
    32761 gggctaaaaa gcgtttccgg gccgaagttt attctgcccg cgattgatgg ctccacggtg
    32821 aagcagaaac tgttcctcgt ggcgctgctg gtgcttgcgg tgtcgtggcc gtttatggtg
    32881 tcacgtggga cggtggatat cgccactctg accatgatct acattatcct cggtctcggg
    32941 ctgaatgtgg ttgttggtct ttctggtctg ctggtgctgg ggtacggcgg tttttacgcc
    33001 atcggcgctt acacttttgc gctgctcaat cactattacg gtctggggtt ctggacctgt
    33061 ttgccgattg ccggactgat ggcagcggcg gcgggcttcc tgcttggttt tccggtgttg
    33121 cgtctgcgcg gtgattatct ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata
    33181 ttgctgctca ataacaccga aattaccggc ggcccgaacg gtatcagtca gatcccgaaa
    33241 ccgactttct tcggccttga gttcagccgt accgcccgtg aaggcggttg ggacacgttc
    33301 agtaatttct ttggcctgaa atacgatccc tccgatcgcg tcatcttcct gtatctggtg
    33361 gcgttgctgc tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg
    33421 gggcgtgcgt gggaagcgtt gcgtgaagat gagatcgcct gccgttcgct gggcttaagc
    33481 ccgcgtcgta tcaagctgac tgcctttacc atcagtgccg cgtttgccgg ttttgccgga
    33541 acgctgtttg ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg
    33601 gcgtttgtac tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg
    33661 gcggcaattt tgctggtggt atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg
    33721 ttaatgctcg gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc
    33781 atgacgcgcc cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc
    33841 agccattatt atctgtgaac ggcctgatga tgcgcttcgg cggcctgctg gcggtgaaca
    33901 acgtcaatct tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg
    33961 gaaaaactac ggtttttaac tgtctgaccg gattctacaa acccaccggc ggcaccattt
    34021 tactgcgcaa tcagcacctg gaaggtttac cggggcagca aattgcccgc atgggcgtgg
    34081 tgcgcacctt ccagcatgtg cgtctgttcc gtgaaatgac ggtaattgaa aacctgctgg
    34141 tggcgcagca tcaacaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt
    34201 tccgtcgcgc ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt
    34261 tgctggaaca cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg
    34321 agattgcccg ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag
    34381 gtcttaaccc aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc
    34441 acaacaccac tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc
    34501 gaatttatgt ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta
    34561 ataacccgga cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg
    34621 tcctttgaca aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg
    34681 catatcaatc agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccacc
    34741 ttgctcggca cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat
    34801 aaagacatta ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg
    34861 gaagggcgtc gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt
    34921 tttgccgaac gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gttcccacgt
    34981 ctgcatgagc gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg
    35041 gcgattggtc gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctt
    35101 ggtcttgcgc cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag
    35161 gggatgacta tcttcctcgt cgagcagaac gccaaccagg cgctaaagct ggcggatcgc
    35221 ggctacgtgc tggaaaacgg ccatgtggtg ctctccgata ctggtgatgc actactggcg
    35281 aatgaagcgg tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc
    35341 aaaagactcc tttgagacag gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag
    35401 gcctactggg tgagttgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt
    35461 ttacgccgca tccggcatga aacgatgagt aatctgtaga gtttgattca gaccttctat
    35521 ttttccgctt atccgtgccc catctcccat tttccctcac ccacgccgtc accgccttgt
    35581 catctttctg acaccttact tgcaggttta tgaagttggc accgccacca tgatggcatc
    35641 gaaagccatt aaaccggtat atgacgtgtt taaagaggca gggattcaat tcgatgagtc
    35701 gcagtttgtg ccgacggttt ccggttacta ctccgacagc aaaacgggcc acttactctc
    35761 ccagccattc aacagctcga ctcccgttct ctattacaac aaagacgcct tcaagaaagc
    35821 ggggttagac ccggaacagc cgccgaaaac ctggcaggat ctggcggact cttccgcaaa
    35881 actgaaagcc tccggtatga agtgcggcta cgccagcggc tggcagggct ggatccaact
    35941 ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg gctttgacgg
    36001 cacggacgcg gtgctggagt tcaacaagcc ggagcaggtg aaacacattg ccatgctcga
    36061 agagatgaac aagaagggcg acttcagcta cgtcggtcgt aaggatgaat ccaccgagaa
    36121 gttctataac ggtgattgcg cgatgaccac cgcctcttcc ggttctctcg ctaatattcg
    36181 tgagtacgcc aaatttaact atggcgtagg catgatgcct tacgacgccg atgcgaaaga
    36241 tgcaccgcaa aacgccatta tcggcggggc cagcctgtgg gtgatgcagg gcaaaaataa
    36301 agaaacgtat accggcgtgg cgaagttcct cgacttcctc gcgaagccag aaaacgctgc
    36361 cgagtggcat cagaaaaccg gttatctgcc aatcaccaaa gcagcgtatg acctgacccg
    36421 tgagcagggc ttttatgaga aaaacccagg ggcggatacc gcgacgcgtc agatgctgaa
    36481 taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc agatccgcgt
    36541 gattgtggat gaagagctgg agagcgtgtg gaccggtaag aagacaccac agcaggcact
    36601 ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat cgacgaagtc
    36661 ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg tgtaggccgg
    36721 ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca tcccgtccgg
    36781 tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc atcaccgtta
    36841 tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gcggcaaagc gtcgatccgt
    36901 ttggtttctc cagccagttt gtcggtctgg ataacttcgt cacgctgttc catgacagct
    36961 actatctaga ctccttctgg acgacgataa aattcagcac ctttgtcacc gtcagcggtt
    37021 tgctggtatc gctgttcttt gcggcgctgg tggagtacat cgtgcgcggc agccgtttct
    37081 atcaaacctt gatgctgctg ccgtatgccg tggctcccgc cgttgccgcc gtattgtgga
    37141 tcttcctgtt taaccccggt cgcgggctga tcacccattt tctcgctgag ttcggctacg
    37201 actggaacca cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt gcctcggtgt
    37261 ggaagcaaat cagctacaac tttctgttct tctatgccgc gctgcactcc attccccgtt
    37321 cgttgattga agccgcagct attgacggcg cagggccgat tcgccgcttc tttaagattg
    37381 ctctgccgct tatcgctccg gtgagtttct tcctgctggt ggtgaacctg gtgtacgcct
    37441 tcttcgacac cttcccggtg atcgacgccg ccacgtccgg cgggccggtt caggccacca
    37501 ctacgctgat ttataagatc taccgcgaag gctttaccgg actggatctg gcttcgtctg
    37561 ccgcgcagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg cagttccgct
    37621 atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga cgatattcag
    37681 ccataccatg ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg tggcgtttgt
    37741 cgcggcgacg ctggataaac aggccgtcta tgccgcgccg atgacgctca tccccggcac
    37801 acatctgctg gaaaatatcc acaacatctg ggttaacggg gtaggcacga atagcgcgcc
    37861 gttctggcgg atgttgctta acagctttgt gatggcgttc agcattacgc tcggcaaaat
    37921 taccgtctcg atgctctcgg catttgccat tgtctggttt cgttttccgc tacgtaacct
    37981 cttcttctgg atgattttta tcaccctgat gctgccggtt gaagtacgta tcttcccgac
    38041 ggtggaagtc atcgccaacc tgaaaatgct cgacagctac gccggtttaa cgctgccgct
    38101 gatggcctcg gcgaccgcca cgtttctgtt ccgccagttc tttatgaccc taccggatga
    38161 attggtggaa gccgcgcgga tcgacggcgc gtcaccaatg cgtttctttt gcgacatcgt
    38221 ttttccgttg tccaaaacta atctggcggc gctgtttgtg atcaccttta tctacggctg
    38281 gaatcagtat ttgtggccgt tgttgattat taccgacgtt gatctcggca ccaccgtggc
    38341 agggatcaaa gggatgatcg ctacaggcga aggcaccacg gaatggaact cggtaatggc
    38401 ggcgatgttg ttaacgctta tccctccggt ggtgattgtt ttagtgatgc agcgtgcctt
    38461 cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta caggcagtaa
    38521 ccaaaagctg ggatggcaaa acccaggtca ttaaaccgct gacccttgat gtggcggatg
    38581 gcgaatttat cgtgatggtc gggccgtctg gctgcgggaa atcgacgctg ctgcgcatgg
    38641 ttgccgggct ggagcgggtg acagaaggcg atatctggat caacgaccag cgcgtgaccg
    38701 aaatggagcc gaaagatcgc gggattgcga tggtgttcca gaattacgcg ctttatccgc
    38761 atatgagcgt agaggagaac atggcgtggg ggctgaaaat tcgcggcatg ggcaagcagc
    38821 aaattgccga gcgcgttaaa gaagcggcgc gcattctgga actggacggt ctgcttaagc
    38881 gtcgtccgcg tgagctatcc ggcggtcagc gccagcgtgt ggcgatgggc cgcgcgattg
    38941 tgcgcgatcc ggcggtgttc ttgtttgatg agccgctctc taacctcgat gccaagctgc
    39001 gcgtgcagat gcgtcttgaa ctgcaacagt tgcaccgtcg cctgaaaacg acttcactct
    39061 acgttactca cgatcaggtt gaagcgatga cgctcgccca gcgagtaatg gtgatgaacg
    39121 gtggcgttgc cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc gccagcctgt
    39181 ttgtggcgag ttttatcggc agcccggcga tgaacctgct gacaggccgc gtgaataatg
    39241 aaggcacgca tttcgaactg gacggcggta tcgtgctacc gctaaacggt ggctaccgtc
    39301 agtatgccgg gcgtaaaatg actctcggca ttcgcccgga acatatagcg ctaagctcgc
    39361 aggcagaagg tggtgtgccg ctggtgatgg acacgctgga gatcctcggc gcagataacc
    39421 tggcgcacgg acgctggggc gagcagaagc tggtggtgcg actggcgcat caggagcgcc
    39481 cgacggcagg cagcacgctg tggctgcatt tggcggaaaa tcagctgcat ctttttgatg
    39541 gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg ctcatcgtgg
    39601 cggcggtaag ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg caaaatacgg
    39661 tcataagatg atcgaatttg acgcgaagtt atcgaaagat ggcgagatct tcctgctcca
    39721 tgacgacaat ctcgaacgca ccagcaacgg ctggggcgtc gcgggtgaac tgaactggca
    39781 ggatttactg cgcgtggatg cgggcagttg gtacagcaaa gcgtttaaag gtgagccgct
    39841 gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg cgaatatcga
    39901 aatcaaaccc accactggca ctggaccatt aacgggcaaa atggtggcgc tggcggcacg
    39961 ccaactgtgg gccggtatga cgccgccgct gctgtcatcg tttgagattg atgctttaga
    40021 agctgcacaa caggcggcac cggaactgcc gcgtggttta ttgcttgatg agtggcgcga
    40081 cgactggcgc gaactgaccg cgcggctggg ctgcgtctct attcatctca atcataagtt
    40141 actcgataaa gcgcgagtga tgcagttgaa agacgccgga ttacggattc tggtttatac
    40201 cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt gcatctgtac
    40261 cgatgcgatt gacgtgattg gtccgaactt tacggcccaa tagttttcaa cggaatgtca
    40321 ggctgcggcg tgctgggctg attcagcatg tcgccgtttc tcctttccgg cagcatatgc
    40381 tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc gttggtgttg
    40441 ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa cacccgctga
    40501 gaattattgt ttatctggtt ttctaaatgc tgctgttgca actgagtttg cgttttcagt
    40561 tgctgattca gcatcccttt ttgctggatt tgctgagtct gcatctgggt ttgcatccgc
    40621 tgctggctgg gtatctgata ccccggctgg ttagggttgt tcagagtatt aatgggctgt
    40681 gcaaagccga caaacggcag gagtgccgta agaatcagaa gtcgtttcat cgcgtatcct
    40741 cctctgaaga tatcctttaa gtttactcgc ttcccgacaa aacgatgatt aattcagagt
    40801 tatataccag gcttagctgg ggttgcccct taatctctgg agaataacga tgataaaacc
    40861 gacgttttta cgccgggtgg ccattgctgc tctgctctca ggaagttgtt ttagcgccgc
    40921 cgccgcgcct cctgcgccgc ccgtctcgta tggtgtggag gaagatgtct tccacccggt
    40981 acgcgcgaaa cagggaatgg tagcgtctgt ggacgccact gccactcagg tgggggtgga
    41041 tatcctcaag gagggcggga atgccgttga tgccgccgtg gcggtgggct acgcgctggc
    41101 ggtaacgcat ccgcaggcag ggaatctggg cggtggtggt tttatgttaa tccgctcgaa
    41161 aaatggcaat accacggcta tcgatttccg cgaaatggca cccgccaaag cgacccgcga
    41221 tatgttcctc gatgatcagg gcaacccgga cagcaaaaaa tcactcactt cgcatctggc
    41281 ttccggcaca ccgggtacgg tagcaggttt ctcgctggcg ctggataaat acggcaccat
    41341 gccgctgaac aaagtcgtgc agcccgcgtt taaactggca cgcgatggtt ttatcgttaa
    41401 cgacgcgctg gctgacgatc tcaaaaccta cggtagcgaa gtgttgccga atcacgaaaa
    41461 cagtaaagct atcttctgga aagagggcga gccgctgaaa aagggcgaca cgctggtgca
    41521 ggcgaacctg gcaaagagcc tggagatgat tgctgaaaac ggcccggacg aattctataa
    41581 aggcacgatt gcggaacaga tcgcccagga gatgcagaaa aacggtggct tgatcactaa
    41641 agaagattta gcggcctata aagcggtcga acgcactccg ataagcggcg attatcgcgg
    41701 gtatcaggtt tactccatgc caccgccatc ctccggcggg atccatatcg tacaaatcct
    41761 caatattctg gaaaacttcg atatgaagaa atacggcttt ggcagcgccg atgcgatgca
    41821 aatcatggca gaagcggaga aatacgccta cgccgaccgc tcggaatatc ttggcgaccc
    41881 ggattttgtc aaagtcccgt ggcaggcact gaccaataaa gcctatgcca aatccattgc
    41941 cgatcaaatt gatatcaaca aagcgaagcc gtccagtgag attcgccctg gcaagcttgc
    42001 gccttatgag agtaatcaaa ctacccatta ctctgtagtg gataaagacg gtaacgcggt
    42061 ggcggtgacc tatacgctga acaccacctt cggtatgggc attgtcgcgg gcgagagcgg
    42121 tattctgctt aataatcaga tggatgattt ctccgccaaa ccgggcgtac cgaacattta
    42181 cgggctggtg ggcggtgatg ccaacgccgt cgggccgaat aaacgtccgc tgtcatcgat
    42241 gtcaccaacc attgtggtga aagacggtaa aacttggctg gttaccggta gtcctggcgg
    42301 gagtcggatt attaccaccg tgc
//
LOCUS       DAZPBM010000018        40244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPBM010000018 DAZPBM010000000
VERSION     DAZPBM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.018"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHUZQ_000864"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHUZQ_000864"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1024638.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUZQ_000865"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUZQ_000865"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1024639.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUZQ_000866"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUZQ_000866"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1024640.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUZQ_000867"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUZQ_000867"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1024641.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUZQ_000868"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUZQ_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1024642.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUZQ_000869"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUZQ_000869"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1024643.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUZQ_000870"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUZQ_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1024644.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUZQ_000871"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUZQ_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1024645.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHUZQ_000872"
     CDS             6537..6773
                     /locus_tag="ACHUZQ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1024646.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHUZQ_000873"
     CDS             6776..7633
                     /locus_tag="ACHUZQ_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1024647.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHUZQ_000874"
     CDS             7654..9645
                     /locus_tag="ACHUZQ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1024648.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUZQ_000875"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUZQ_000875"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1024649.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHUZQ_000876"
     CDS             complement(10248..11144)
                     /locus_tag="ACHUZQ_000876"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1024650.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHUZQ_000877"
     CDS             complement(11193..11432)
                     /locus_tag="ACHUZQ_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1024651.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUZQ_000878"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUZQ_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1024652.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUZQ_000879"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUZQ_000879"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1024653.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUZQ_000880"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUZQ_000880"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1024654.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUZQ_000881"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUZQ_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1024655.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHUZQ_000882"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHUZQ_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUZQ_000883"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUZQ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1024656.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUZQ_000884"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUZQ_000884"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1024657.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUZQ_000885"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUZQ_000885"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1024658.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22745)
                     /locus_tag="ACHUZQ_000886"
     CDS             complement(21570..22745)
                     /locus_tag="ACHUZQ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1024659.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22911..23000)
                     /gene="cydH"
                     /locus_tag="ACHUZQ_000887"
                     /gene_synonym="ynhF"
     CDS             complement(22911..23000)
                     /gene="cydH"
                     /locus_tag="ACHUZQ_000887"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1024660.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23299..24324
                     /gene="purR"
                     /locus_tag="ACHUZQ_000888"
     CDS             23299..24324
                     /gene="purR"
                     /locus_tag="ACHUZQ_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1024661.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24321..25253)
                     /gene="punR"
                     /locus_tag="ACHUZQ_000889"
     CDS             complement(24321..25253)
                     /gene="punR"
                     /locus_tag="ACHUZQ_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1024662.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25366..26577
                     /gene="punC"
                     /locus_tag="ACHUZQ_000890"
     CDS             25366..26577
                     /gene="punC"
                     /locus_tag="ACHUZQ_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1024663.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYCGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26868..28016
                     /gene="cfa"
                     /locus_tag="ACHUZQ_000891"
     CDS             26868..28016
                     /gene="cfa"
                     /locus_tag="ACHUZQ_000891"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1024664.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28056..28697)
                     /gene="ribE"
                     /locus_tag="ACHUZQ_000892"
     CDS             complement(28056..28697)
                     /gene="ribE"
                     /locus_tag="ACHUZQ_000892"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1024665.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28912..30285
                     /gene="mdtK"
                     /locus_tag="ACHUZQ_000893"
     CDS             28912..30285
                     /gene="mdtK"
                     /locus_tag="ACHUZQ_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1024666.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30326..31582)
                     /locus_tag="ACHUZQ_000894"
     CDS             complement(30326..31582)
                     /locus_tag="ACHUZQ_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1024667.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31890..31966
                     /locus_tag="ACHUZQ_000895"
     tRNA            31890..31966
                     /locus_tag="ACHUZQ_000895"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31924..31926,aa:Val,seq:gac)
     gene            31971..32047
                     /locus_tag="ACHUZQ_000896"
     tRNA            31971..32047
                     /locus_tag="ACHUZQ_000896"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32005..32007,aa:Val,seq:gac)
     gene            32155..32460
                     /locus_tag="ACHUZQ_000897"
     CDS             32155..32460
                     /locus_tag="ACHUZQ_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1024668.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32586..34190
                     /locus_tag="ACHUZQ_000898"
     CDS             32586..34190
                     /locus_tag="ACHUZQ_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR1024669.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34202..35014)
                     /gene="ydhT"
                     /locus_tag="ACHUZQ_000899"
     CDS             complement(34202..35014)
                     /gene="ydhT"
                     /locus_tag="ACHUZQ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1024670.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35018..35803)
                     /gene="phsC"
                     /locus_tag="ACHUZQ_000900"
     CDS             complement(35018..35803)
                     /gene="phsC"
                     /locus_tag="ACHUZQ_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1024671.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35800..36468)
                     /locus_tag="ACHUZQ_000901"
     CDS             complement(35800..36468)
                     /locus_tag="ACHUZQ_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1024672.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36532..37170)
                     /locus_tag="ACHUZQ_000902"
     CDS             complement(36532..37170)
                     /locus_tag="ACHUZQ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1024673.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37183..39285)
                     /locus_tag="ACHUZQ_000903"
     CDS             complement(37183..39285)
                     /locus_tag="ACHUZQ_000903"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR1024674.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="ACHUZQ_000904"
     CDS             complement(39306..39932)
                     /locus_tag="ACHUZQ_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1024675.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9674 a        10561 c         9905 g        10104 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagcg ccagcaacgg atagttaatt
    22741 ttcatgcctt atctccacct cttcgcgtca ttacgcgata ttcattaaag tggcgaaagc
    22801 atgacagcaa tcacaaaaaa atgaaaataa caaaaagaga aagcactttt gccattttgc
    22861 taacaaacag gaaggagatg cgagggagaa cgcgctccct cgagaggaaa tcagtgcagc
    22921 gcggcagtca aacccacggc tacgatcaaa ccgaggacga taatcgttgt taccagtgaa
    22981 aatttaaggt cggtgctcat caagttttct ccttttttat taccacacaa aaagtgatat
    23041 tacgcatttt tacacactgt gatgaaaaaa tctcccgtca tttataatga taagtgtttt
    23101 taccacttcc ccttttcgtc aagatcggcc aaaattccac gcttacacta tttgcgtact
    23161 ggccattgac cccttcctga cgctccgtgt cgtttttccg gcgtaccgca acacttttgt
    23221 tgtgcgtaag gtgtgtaaag gcaaacgttt accttgcgat tttgcaggag ctgaagttag
    23281 ggtctggagt gaaatggaat ggcaacaata aaagatgtag cgaaacgagc aaacgtttcc
    23341 actacaactg tgtcacacgt gatcaacaaa acacgtttcg tcgctgaaga aacgcgcaac
    23401 gccgtgtggg cagcgattaa agaattacac tactccccta gcgcggtggc gcgtagcctg
    23461 aaggttaacc acaccaagtc tatcggtttg ctggcgacca gcagcgaagc ggcctatttt
    23521 gccgagatca ttgaagcagt tgaaaaaaat tgcttccaga aaggttatac cctgattctg
    23581 ggcaatgcgt ggaacaatct tgagaaacag cgggcttatc tgtcgatgat ggcgcaaaaa
    23641 cgcgtcgatg gtctgctggt gatgtgttct gagtacccag agccgttgct ggcgatgctg
    23701 gaagagtatc gccatatccc aatggtggtg atggactggg gtgaagcaaa agctgacttc
    23761 accgatgcgg tcattgataa cgcgttcgaa ggcggctaca tggccgggcg ttatctgatt
    23821 gaacgcggtc accgcgaaat cggcgttatc cccggcccgc tggaacgtaa cactggcgca
    23881 ggccgccttg tcggttttat gaaggcgatg gaagaagcaa tgatcaaggt gccggaaagc
    23941 tggattgtgc agggtgactt tgaacctgaa tccggttatc gcgccatgca gcaaatcctg
    24001 tcgcagccgc atcgccctac tgccgtcttc tgtggtggcg atatcatggc aatgggcgca
    24061 ctttgtgctg ctgatgaaat ggggctgcgc gtcccgcagg atgtttcgct aattggttat
    24121 gataacgtgc gcaacgcccg ctattttacg ccggcgctga ccacaatcca tcagccaaaa
    24181 gattcgctgg gtgaaacagc gttcaacatg ctgttggatc gtatcgtcaa caaacgtgaa
    24241 gaaccgcagt ccattgaagt gcatccgcgc ttgattgaac gccgctccgt ggctgacggc
    24301 ccgttccgcg actatcgtcg ttaatcaccc gttgcgggag tctcttccgg ctcccgcagc
    24361 cactccttat tcagcgtctc actatcgccg agatactcaa gcaaccaggt taacgcaggc
    24421 gacatatcat tttgctgcca tgtcagacaa catgccgaat ccggaaaggg gttttccagt
    24481 tcaagtgcta cccacttccc ctcattaagc cacggtttgg cgaaatgtgt tggcaccatc
    24541 cctatgcata atcctgccga gatacaggtt gccgatgatt cccagtcagg cacgacaact
    24601 cttttttggt tatccagcaa ccaggtaata cgtttaggta gcgttcgcga ggtgtcttcg
    24661 cgcaccaacg acggccagtt gcgcaacgta tcatcgctga acgggccatc catcaacgcc
    24721 agcgggtgat ggctggcaac aacgcaactc cagcttagca tccccatatc ccggaaggca
    24781 taacgaccgc ctaccggaat cgcgcgtgtt gcgccaatcg ccagttccac gcgcccgtcg
    24841 gaaagcgcat cccagacacc gttgaacact tcctgaaaga caagaagttc gacatcatca
    24901 aaatggcgat aaaaatcaac gatcatctgc cgtgtacgtt ctggcctgac aatattatcc
    24961 actgcgatag ctaactgacc gcgccagccg ttcgctatct gctgacattg ctggcgggtg
    25021 atctgcattt ttttgacaac agagcgccct tctttgagaa accacgctcc agcagcggtc
    25081 agctccacat cacggtgccg tcgttcaaag agcggcaccg ccagccactc ttccagctga
    25141 cgcacggtat agctgaccgc agaaggaacg cgatgcagct cctgtgccgc agcgctaaaa
    25201 ctaccattac gcgctaccgc atcaacaact tcgagtgaat attctgacca catagtctgc
    25261 ctgcaaaatt tttgaaacca atcatcaaat attaccgttt cacaacacta atttcactcc
    25321 ctacactttg cggcggtgtt taattgagag atttagagaa tatacatgca acctgggaaa
    25381 agatttttag tctggctggc gggtttgagc gtactcggtt ttctggcaac cgatatgtat
    25441 ctgcctgctt tcgccgccat acaggccgac ctgcaaacgc ctgcgtctgc tgtcagtgcc
    25501 agccttagtc tgttccttgc cggttttgcc gcagcccagc ttctgtgggg gccgctctcc
    25561 gaccgttatg gtcgtaaacc ggtattatta atcggtatga caatttttgc gttaggtagt
    25621 ctggggatgc tgtgggtaga aaacgccgct acgctgctgg tattgcgttt tgtacaggct
    25681 gtgggtgtct gcgccgcggc ggttatctgg caagcgttag tgacggatta ttatccttca
    25741 cagaaagtta accgaatttt tgcgaccatc ataccgctgg tgggtctatc tccggccctg
    25801 gctcctctgt taggaagctg gctgctggtc catttttcct ggcaggcgat tttcgccacc
    25861 ctgtttgcca ttaccgtggt gctgattctg cctattttct ggctcaaacc cacgacgaag
    25921 gcccgtaaca atagtcagga tggtctgacc tttaccgacc tgctacgttc taaaacctat
    25981 tgcggcaacg tgctgatata cgcggcctgt tcagccagtt tttttgcatg gctgaccggt
    26041 tcaccgttca tccttagtga aatgggctac agcccggcag ttattggttt aagttatgtc
    26101 ccgcaaacta tcgcgtttct gattggtggt tatggctgtc gcgccgcgct gcagaaatgg
    26161 caaggcaagc agttattacc gtggttgctg gtgctgtttg ctgtcagcgt cattgcgacc
    26221 tgggctgcgg gcttcattag ccatgtgtcg ctggtcgaaa tcctgatccc attctgtgtg
    26281 atggcgattg ccaatggcgc gatctaccct attgttgtcg cccaggcgct gcgtcccttc
    26341 ccacacgcaa ctggtcgcgc cgcagcgttg cagaacactc tacaactggg tctgtgcttc
    26401 ctcgcaagtc tggtagtttc ctggcttatt agtatcagca cgccattgct caccaccacc
    26461 agcgtgatgt tatcaacagt agtgctggtc gcgctgggtt acatgatgca acgttgtgaa
    26521 gaagctggct gccagaatca tggcaatgcc gaagtcgctc atagcgaatc acactgatct
    26581 atatcgatat acttatactt aggctgctaa caaaattttg ttgtatgatt gaaattagcg
    26641 gcctatacta atttcgagtt gttaaagcta cgataaatat tatgttttta cggggacagg
    26701 atcgttcccg actcactatg gatggtcatt tcggcaaggg ttcctccttt ccctctgttc
    26761 tacgtcggat tatagactcg cggttttttc tgcgagattt ctcacaaagc ccaaaaagcg
    26821 tctacgctgt tttaaggttc tgatcaccga ccagtgatgg agaaactatg agttcatcgt
    26881 gtatagaaga agtcagtgta ccggatgaca actggtaccg tatcgccaac gaattactta
    26941 gccgtgccgg tatagccatt aacggttctg ccccggcgga tattcgtgtg aaaaaccccg
    27001 atttttttaa acgcgttctg caagaaggct ctttggggtt aggcgaaagt tatatggatg
    27061 gctggtggga atgtgaccga ctggatatgt tttttagcaa agtcttacgc gcaggtctcg
    27121 agaaccaact cccccatcat ttcaaagaca cgctgcgtat tgccggcgct cgtctcttca
    27181 atctgcagag taaaaaacgt gcctggatag tcggcaagga gcattacgat ttgggtaatg
    27241 acttgttcag ccgcatgctt gatcccttca tgcaatattc ctgcgcttac tggaaagatg
    27301 ccgataatct ggaatctgcc cagcaggcga agctcaaaat gatttgtgaa aaattgcagt
    27361 taaaacctgg gatgcgcgta ctggatattg gctgcggctg gggcggactg gcacactaca
    27421 tggcatctaa ttatgacgta agcgtggtgg gcgtcaccat ttctgccgaa cagcaaaaaa
    27481 tggctcagga acgctgtgaa ggcctggatg tcaccatttt gctgcaagat tatcgtgacc
    27541 tgaacgacca gtttgatcgt attgtttctg tggggatgtt cgagcacgtc ggaccgaaaa
    27601 attacgatac ctattttgcg gtggtggatc gtaatttgaa accggaaggc atattcctgc
    27661 tccatactat cggttcgaaa aaaaccgatc tgaatgttga tccctggatt aataaatata
    27721 tttttccgaa cggttgcctg ccctctgtac gccagattgc tcagtccagc gaaccccact
    27781 ttgtgatgga agactggcat aacttcggtg ctgattacga tactacgttg atggcgtggt
    27841 atgaacgatt cctcgccgca tggccagaaa ttgcggataa ctacagtgaa cgctttaaac
    27901 ggatgtttac ctattatctg aatgcctgcg caggtgcttt ccgcgcccgt gatattcagc
    27961 tctggcaggt cgtgttctca cgcggtgttg aaaacggcct tcgagtcgct cgctaaaggc
    28021 tattctatcg ccccctctcc gggggcgatt tcagatcagg cttctgtacc tggttgattc
    28081 atggcatttt ctcgtgccgc cagcacacgt tctaccgtat ctaccactgc ctgagtttgt
    28141 ggatcaattt caatgttgac gcgtgcgccg agttttttct tcccaagagt cgtgcgttcc
    28201 agagtttccg gaattaaatg gacgcaaaaa cgcgttggcg tgacttcgcc gacggtcagg
    28261 ctaataccgt cgatgccaat aaatcctttg tacaggatat atttcatcaa ctgactatcc
    28321 tggactttaa accagatctg gcgattattt tccgaggtta atattttcgc cacttcagcg
    28381 gtggtcataa tatgacctga cattaagtgt ccgccaattt catcgctgaa ttttgccgca
    28441 cgctcaacgt ttacccaatc ccccactttt aaatcgccaa gattggtaat gcgtaacgtt
    28501 tctttcatca ggtcaaaact gacatggttg ccgttaattt ccgtcacggt caggcagcaa
    28561 ccgttatgcg ccacggaagc acctgtttcc aggccgtcca gcatgtggtc gggtaactcc
    28621 accacatgcg tacgaaaatt tggtttctcg tcaatcgaca ccagttttgc ggtgccctgt
    28681 acaatccccg taaacatact tacaactcct gaaatcagtt aagacattct gttcagcaca
    28741 atagcaggtg gaaaacgccc ttaccagtga aggggtaaga atgactattt tttcactgga
    28801 gaattaataa atcctcgcta caatagactg aatttcccct gcttcttctt tttgctgccc
    28861 attcaggcgg ctttttagtc tctcatataa ctacaaataa aaggtgttca cgtgcagaag
    28921 tatatcagtg aagcgcgtct gttattagca ttagcaatcc cggtgattct cgcgcaaatc
    28981 gcccaaactg cgatgggttt tgtcgatacc gtgatggcgg gcggctatag tgccaccgac
    29041 atggcggcgg tcgctatcgg tacttctatc tggcttccgg cgatcctctt tggtcacgga
    29101 ctgctgctgg cattaacgcc ggttatcgcg caattaaatg gttccggtcg acgtgagcgc
    29161 attgcgcatc aggtgcgaca aggtttctgg ctggcaggtt ttgtttccgt tctcattatg
    29221 ctggtgctgt ggaatgcagg ttacattatc cgctccatgg aaaacatcga tccggctctg
    29281 gcggacaaag ccgtgggtta tctgcgtgcg ttgttgtggg gcgcgccagg ctatcttttc
    29341 ttccaggttg cccgtaacca gtgtgaaggt ctggcaaaaa ccaagccggg tatggtaatg
    29401 ggctttatcg gcctgctggt gaacattccg gtgaactata tctttattta tggtcatttc
    29461 ggtatgcctg agctcggtgg cgttggttgt ggcgtggcta ctgcggcggt gtattgggtt
    29521 atgttccttg ccatggtttc ttacattaaa cgcgcccgct ccatgcgcga tatccgtaac
    29581 aaaaaaggca ccgcaaaacc cgatcctgcg gttatgaaac gactgattca actcggtttg
    29641 ccgattgcgc tggcactatt ctttgaagtg acactgtttg ccgtcgtggc tctgttagtg
    29701 tctccgctcg gtattgttga tgtcgcagga caccagattg ccctgaactt tagttcacta
    29761 atgttcgtgc ttccaatgtc gctggcggca gcggtaacta tccgcgtagg ttatcgtctg
    29821 ggtcagggct caacgctgga tgcgcaaacc gctgctcgga ccgggcttat ggtgggtgtc
    29881 tgtatggcaa ccctgacggc cattttcacg gtttcactgc gggagcaaat cgccctgttg
    29941 tacaacgaca atcccgaggt tgtaacgctg gctgcgcatt tgatgttgct ggcggcggta
    30001 tatcagattt ctgactcaat ccaggtgatt ggcagtggga ttttgcgtgg ttataaagat
    30061 acgcgttcca ttttctatat cacctttaca gcttactggg tgctgggctt gccaagcggc
    30121 tatattctgg cactgaccga tctggtcgtt gaacctatgg ggccagcagg cttctggata
    30181 ggctttatta tcggcctgac atcggcagcc attatgatga tgctgcgtat gcggttcctg
    30241 caacgtatgc cgtcagccat cattctgcaa cgagcgtccc gctaataaag acaaggcgca
    30301 accttcacgg gttgcgcctg tgtttttacg caggctggag cgttgcgcca atcccgtctt
    30361 cgtctggctg taatttcaga gcgttacaca gagtttgccg actggcatct gttatctcaa
    30421 cgcgtatatc cccgttaact tcccctttca cattgatgaa gtcaggtgaa atttctttcg
    30481 cactggtttg caggcaaatc gctccctctc cgcgcaggcg tttcactttc agttgcacat
    30541 gcgttgccgc accagtgatc agccacagtt ttccgccatt cagattgatg ttaccaacaa
    30601 tgaaagtgac gctgtcacct tttaccgtac tgccgtcgag aatcaacaca ccgccctgct
    30661 gtacagtaat cgttgattgt tgcagctctg cgccataacc aataatcatt gaagcgccgt
    30721 ccagtaatgc aacactcccg gtcagttcaa ccggtgcgcg atcttcctct acgacaaaat
    30781 tttcgtccgc gctggtgggt gacaacagga tcactgttcc accttgtatt accgcatttt
    30841 ccagcgtacc ggcatgcact tcagcccgcc cccccacatt caccgacagg ttttgcgtct
    30901 taccggaact gtagagctga aggccatccg tccccagacg ataaatggat ccattctcaa
    30961 caatagtgtc agagtcttca ccaccgagat tattcatggt gccaccagca agcaacgtat
    31021 cactggcttt cccggccagc ataaaaacgc cgccctgatt aatctcacaa ttgctggctt
    31081 ctccacttgg atacaccaac aattcaccac cttcatctac cacgaccgct gaggttgtcc
    31141 cattaaccaa caggccgccc tcttgatcga gaatgatttt ttccgcgcga tgtccttcca
    31201 gtacacgcag gttaccgcca ttttccagca acaaaccgca ggcataaccc tgatcaacgc
    31261 tgaattcacc ttcgggatgg cggccattaa ccgtagcgag cgtagagaga ctaattgcgc
    31321 ctccctcgga ctgaacaata tctgtggcaa tggcaccgtc gctgaccgat agcgtgccat
    31381 tgttaccgta ctgtgtgcat ttggcgatcc caccggttgc cactttcaac cagccagccc
    31441 ccgtgaccac ggtattttcg gctcttccgc cagcttcaac aatcaactcg ccttcagtaa
    31501 gttgcgtcgc gccaatgacc tcgccggtct taacaatttg cacattcccg tcgctcatca
    31561 agtttttcgc atcagatccc atgatttatt cctttgctgc atctgtgtgc ctttattgct
    31621 acctaagtgt aaaggctacg gaggatttat ccacgacaga tttgagatgg tggcaaacaa
    31681 ttctgtttaa actctgatac acgaattatt gggttgtatc agatgtaaat gcgatcctga
    31741 ataaaaatcg cccttgcaaa tcaacaaaat atagccaatt tgcttaaaag ctctgcaaac
    31801 ggtcgaaata gcgcagaaaa ttacgttttg cctcttgcca cctttccact ctgccgctaa
    31861 tatttgtccc cgttgtcacc tacaacgttg cgttcatagc tcagttggtt agagcaccac
    31921 cttgacatgg tgggggtcgt tggttcgagt ccaattgaac gcaccatcct gcgtccgtag
    31981 ctcagttggt tagagcacca ccttgacatg gtgggggtcg gtggttcgag tccactcgga
    32041 cgcaccagat tttcttaatc tggtcttttc ctttttccct ctgtttattc tctgtatcca
    32101 atacgttaaa agatttacac tgtcttcgta tgcgttatca gaaggagaat cgctatggca
    32161 actttgttac aacttcattt tgcttttaat ggcccatttg gcgacgcaat ggctgagcag
    32221 cttaaaccac ttgctgagtc gattaatcag gaacctggtt ttctgtggaa ggtatggaca
    32281 gaaagtgaaa agaaccacga agccggtggg atctaccttt tcactgatga aaaaagcgcc
    32341 cttgcctatc tggaaaaaca tactgcccga ctgaaaaatc tcggcgttga ggaagttgtc
    32401 gccaaagttt tcgatgtcaa tgagccactt agtcaaatca atcaggcaaa actcgcctga
    32461 cagaatttaa tcaagggcgg ttagcgccct tttcatccct gtctgaaatt tctcaaattc
    32521 taaaaatctc aaccaaactt atctgataac actaaattcg aaagaatgcg tacaggtaag
    32581 taacaatgaa aaaaattgct attgtgggtg ccgggcctac ggggatctac accttattct
    32641 cgcttctaca gcaacaaact ccactttcta tttctatctt cgagcaggct gacgaggccg
    32701 gtgtcgggat gccatacagt gatgaggaaa actcaaaaat gatgctggca aatattgcca
    32761 gtattgaaat accgccgatt aattgtacgt atctcgaatg gctacaaaag caagaagcca
    32821 gccatctcca gcgttatggc gttaaaaaag aaaccttgca cgatcgtcag tttttaccgc
    32881 gaattctgct gggcgaatat ttccgcgatc aatttttacg attagtagac caggcacgac
    32941 agcaaaaatt tgcagtggct gtttatgaat catgccaggt taccgatctg caaattacaa
    33001 atgctggcgt catgctcgct acaaatcagg atttacccag cgagacgttt gatttagcgg
    33061 tgattgccac gggtcacgtc tggcctgatg aagaagaagc aaccagaacg tattttccca
    33121 gcccgtggtc aggcctgatg gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa
    33181 catccttgag cggactggat gcggcaatgg cagtggctat tcagcatggt tcgttcattg
    33241 aagatgataa acaacacgtc gtttttcacc gcgataacgc aagtgaaaag ctaaatatca
    33301 cgttgatgtc gcgcacgggt attttacccg aagccgattt ctattgccct attccctacg
    33361 agcccttaca catcgtcacc gatcaggcat taaatgctga gattcaaaaa ggcgaatatg
    33421 gccttttgga tcgggtattt agattgatag tagaggaaat caagtttgct gatccagact
    33481 ggagccaacg catagcctta gagagcctga atgtcgattc ctttgctcaa gcctggtttg
    33541 ccgagcgcaa acaacgcgac ccatttgact gggcagaaaa aaatctccag gaagtcgaac
    33601 gcaataaacg agaaaaacat actgttccct ggcgttatgt cattctgcgc ctgcatgaag
    33661 ccgtacagga aattgttcca catctgaatg aacacgacca taaacggttc agtaaaggcc
    33721 ttgcccgggt tttcatcgat aattatgcgg caatcccttc agagtctatt cgtcggctgc
    33781 tggccttacg tgaagcgggg atcattcata ttctcgccct cggtgaagac tacaaaatgg
    33841 aaattaatga gtcgcgcacc gtcctgaaaa cggaagacaa cagctactcg tttgacgttt
    33901 ttattgatgc ccgcggacag cgtccgctta aagtgaaaga tattcctttc cctgggctac
    33961 gcgagcaatt acagaaaaca ggggatgaaa tccctgatgt tggcgaagat tatacgttac
    34021 agcaacccga agatattcgt ggacgcgtag cgttcggcgc gttgccctgg ttgatgcacg
    34081 accagccttt cgttcaggga cttacggcat gtgcagaaat tggtgaggcg atggctcggg
    34141 cggtcgtaaa gcctgcatcc cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct
    34201 atcagcactc aacaaactga tgctgttcat taagacgata gcggactccg gttcgaatat
    34261 tgttttcacc ttcaatggcg acggcgaagc gaactctttc gccgtcatga tatgccagtg
    34321 cggcgcttcc ccctggcccg agaataattg agtcgaccac gcccgtgctc actaccgttg
    34381 aattttcacc gctggcaatg atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg
    34441 cgccaaagct ggcgatttgc accaggtcac cattactggc gacatgacac cgttcaccca
    34501 atgtacaaac gcgtactcgc attccggtat tcgcaatccg actactatct ccagcgctgc
    34561 ttatccgcga agaattaccg gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt
    34621 gactgttaaa gccgacactt ccaatccgca cgctataacc catactggca atttgcgcgg
    34681 catacccagc ggttacgatt cgggcgttat cctcacaaca gccgatgcga gcatgatcgt
    34741 cgctgcgggc aaactgactg caaaatggta tctctccggg gtctgtggcc agtttttgca
    34801 tagaggaaac ttcctgatgc gcgaagtttt catccgccag ccattttttc cacgcatact
    34861 caaccagact ctccgcccag tccgtatatc cttcctcaat cagtgcatga tgaatatcgg
    34921 cataactacc gccgcgtggg aaatggcgca ggaaccagcg atacatcacc gctccgatac
    34981 gccattctcg caggtctgct cgtgtaatga tcattcgtta gtgccggtga tagccatcga
    35041 ccatgctttt aaaggtttcg tgtggcgtac gccccgtggt gcaaagataa agatgtccga
    35101 agataaaaaa gaggcttata aatgccagag caaaatgcgc ctgcaataac cagtatctta
    35161 cgccaggaaa cacatctccc acggcttgcg gatagagaca caacagcccc gtcaatagta
    35221 acaacggcag caatccacac atgacaccaa cataggcgac ctgctgtaag ggattaaatt
    35281 tagactgtgt tgttgccggg aaaggatgtt cttccccctg cataatgcca aacaaataaa
    35341 atcgtgtttg ttttgccgct cgttccagcc acccctgacg acgaatgcga tagtggtgac
    35401 cattatcccc aacggcattg atcagcacaa agccgagcca gcatgccagt aacaaaaatc
    35461 cgcaaacttc atgcaccgca accagacttt taaccgcagt tgcgcccacc atcgcaaagt
    35521 gatttatcag cccacttgcc agcaacaata caaagagtaa cgcattcgac cagtgccata
    35581 atctgaccgc tttgctgtaa agataaacct tctcaccatg atcggtggct gatttcttca
    35641 cgccacgagc acgaagcaag gcgtgcagcc ccaacaccaa ccacatccca accagcaata
    35701 ctccggcaat gatcaaccac accggccaaa attcgggagt gaactgtggc acatagttcg
    35761 ctaactggct ctgaaactgt tcagcatgtt gcgacgggtt catacatttt cctttttaat
    35821 caaatgttga ccgaaccggc gatacagatg cggtttcccg gcaccaagta gctgatattg
    35881 atagtattta ttttcctgca accacgcctg aatttctggg ctatcttcgc gaccaaagat
    35941 caatgcgtgt tccggacagg cgctgacgca aatgggcgga aagccttttg ccagtcggga
    36001 ctcagcgcag aaatcacatt tatctgccac tttagtgaca gggttaaggt agcgaacctg
    36061 gtacggacaa gcgccaatgc aataactgca gccgatgcac tgcgattttt ctacccgcac
    36121 gatcccctgc tcatcacgcc acgacgcccc tgtcgggcat acgtcgatgc acggagcatc
    36181 ttcgcagtgc tgacatgact ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt
    36241 gactggaatg tgtgctatcg ataaacggct tccctgagcg gggacgtggt tagttttacg
    36301 gcatgcgcgg gcgcaaatat tacagccatt gcaccgtgac tcgtcatgaa tcatggcata
    36361 ccgaacttcc ttttcttgcc tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa
    36421 gattactgtt cccatcccca gaacaaattt gcgtcgagtg aaggacatct gattatcccc
    36481 taatgcagtg gctgcaactg tccaccctat ccatgacatc tccctgaaat ttcaggggtt
    36541 ctgaaactgc tctacctcga accattcgca taagccataa gccttgccat aggtcataaa
    36601 tcgctcgcta aacaagtaca acaccgccgt cgatgactcc acctgccgta tatcggcata
    36661 ttcaggtgca acatccatcg cggcgatcgc ggcttcaatc tgcgcttcct ggaagtaata
    36721 tggcgcttgc atcagcatgg cgactttgta aggacgagga tacgtctggc actcaaaacg
    36781 aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca acttgcaaag acatcgccgc
    36841 gtagttttca ctcatggcct gcgttgagta atagtaatca tcctgacttc ccgtcataca
    36901 agcgatatcc gcgtagtctc cgttctgctt aatgctttcc agcaaaactg acagttcctc
    36961 ttctgcaacc gaatagggtg gcgagagaaa cacggtgcgc gccaccagct gcccagccgc
    37021 agaatgctgg cgaatgaact cagccaggat ttcagcaggt tccggttcgg ttaccgtatc
    37081 aacggggtga cgcatcccct gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt
    37141 cgctaacggc aattcatcct gatggttcat cttacccatt ccttacgcag gcagtagatt
    37201 gtgtgctgcc agatcggcgg caatatcttc cagccccaga cgctgcaatg tttcgcgggt
    37261 tggacaacca agctgtgggt cccagcccat ttctttgtag aacatcgtga gcgaagcatg
    37321 catatcgtca cgatccattt tgtcagtacc ttcagtaaag accgggatct gcggatcctt
    37381 gtcgaaaacc caggaacaga taagatcgtg ttcattacgc atatctttgg tttgcatcag
    37441 ttttaccgtg taggcacgat gcaacgtaaa gatacgctct gcagctaaat ctaatttttc
    37501 ctgagtcatc tcttcgccgg tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc
    37561 ccggtaatta cggcttttca gcggcgaaac ggtcattggc cagacccagt tgcacagggt
    37621 gacggcgtta tgcaaacaga cccgcaacag cgaccacttt gcatatttaa tttttgcgtc
    37681 attgattggc gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa aaagttcttt
    37741 cgccacttca cgttgcagtt tcaatggcaa gccggaacca ataaagttga tatgggtatg
    37801 cgtcatgcag tcacggttga acatacagtt aacaatggaa ccgacctgcg ctgacgcttc
    37861 attggcatgg tgaaccggat agccaaacgg cgaccagagt ttatttttcg cgtagcccca
    37921 gtactcttca cccaaattcc agcgttctgc gatggcatat gaaccatcag ccaggtgact
    37981 cagctcaccc acacgatgcg ccagacggta gtaaaaatct ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg
    40141 ttatcaatta aaattacaaa gcaagaaatt ataagtgaac tgatatttat tatcaattga
    40201 aataaattta acttaagcga caattatttg tgacgttcat tgca
//
LOCUS       DAZPBM010000019        39964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPBM010000019 DAZPBM010000000
VERSION     DAZPBM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.019"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..115
                     /locus_tag="ACHUZQ_000905"
     CDS             <1..115
                     /locus_tag="ACHUZQ_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFR1024676.1"
                     /translation="SPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
     gene            complement(202..877)
                     /locus_tag="ACHUZQ_000906"
                     /pseudo
     CDS             complement(202..877)
                     /locus_tag="ACHUZQ_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1022..1630)
                     /locus_tag="ACHUZQ_000907"
     CDS             complement(1022..1630)
                     /locus_tag="ACHUZQ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1024677.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1814..2131)
                     /gene="metJ"
                     /locus_tag="ACHUZQ_000908"
     CDS             complement(1814..2131)
                     /gene="metJ"
                     /locus_tag="ACHUZQ_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1024678.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2408..3568
                     /gene="metB"
                     /locus_tag="ACHUZQ_000909"
     CDS             2408..3568
                     /gene="metB"
                     /locus_tag="ACHUZQ_000909"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1024679.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3571..6003
                     /gene="metL"
                     /locus_tag="ACHUZQ_000910"
     CDS             3571..6003
                     /gene="metL"
                     /locus_tag="ACHUZQ_000910"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1024680.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6142..6276)
                     /locus_tag="ACHUZQ_000911"
     CDS             complement(6142..6276)
                     /locus_tag="ACHUZQ_000911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024681.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6352..7242
                     /gene="metF"
                     /locus_tag="ACHUZQ_000912"
     CDS             6352..7242
                     /gene="metF"
                     /locus_tag="ACHUZQ_000912"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1024682.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7571..9751
                     /gene="katG"
                     /locus_tag="ACHUZQ_000913"
     CDS             7571..9751
                     /gene="katG"
                     /locus_tag="ACHUZQ_000913"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1024683.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9845..10750
                     /gene="yijE"
                     /locus_tag="ACHUZQ_000914"
     CDS             9845..10750
                     /gene="yijE"
                     /locus_tag="ACHUZQ_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1024684.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10777..11394)
                     /locus_tag="ACHUZQ_000915"
     CDS             complement(10777..11394)
                     /locus_tag="ACHUZQ_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR1024685.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11668..12771)
                     /gene="gldA"
                     /locus_tag="ACHUZQ_000916"
     CDS             complement(11668..12771)
                     /gene="gldA"
                     /locus_tag="ACHUZQ_000916"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR1024686.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12782..13444)
                     /gene="fsa"
                     /locus_tag="ACHUZQ_000917"
     CDS             complement(12782..13444)
                     /gene="fsa"
                     /locus_tag="ACHUZQ_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1024687.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13456..15957)
                     /gene="ptsP"
                     /locus_tag="ACHUZQ_000918"
     CDS             complement(13456..15957)
                     /gene="ptsP"
                     /locus_tag="ACHUZQ_000918"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1024688.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16266..17345
                     /locus_tag="ACHUZQ_000919"
     CDS             16266..17345
                     /locus_tag="ACHUZQ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1024689.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17360..17680
                     /locus_tag="ACHUZQ_000920"
                     /pseudo
     CDS             17360..17680
                     /locus_tag="ACHUZQ_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17731..20027
                     /locus_tag="ACHUZQ_000921"
                     /pseudo
     CDS             17731..20027
                     /locus_tag="ACHUZQ_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19993..20871
                     /locus_tag="ACHUZQ_000922"
     CDS             19993..20871
                     /locus_tag="ACHUZQ_000922"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1024690.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20873..21214
                     /locus_tag="ACHUZQ_000923"
     CDS             20873..21214
                     /locus_tag="ACHUZQ_000923"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1024691.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21201..22052)
                     /locus_tag="ACHUZQ_000924"
     CDS             complement(21201..22052)
                     /locus_tag="ACHUZQ_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1024692.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22267..24000)
                     /locus_tag="ACHUZQ_000925"
     CDS             complement(22267..24000)
                     /locus_tag="ACHUZQ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1024693.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24183..26834)
                     /gene="ppc"
                     /locus_tag="ACHUZQ_000926"
     CDS             complement(24183..26834)
                     /gene="ppc"
                     /locus_tag="ACHUZQ_000926"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1024694.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27186..28337)
                     /gene="argE"
                     /locus_tag="ACHUZQ_000927"
     CDS             complement(27186..28337)
                     /gene="argE"
                     /locus_tag="ACHUZQ_000927"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1024695.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28491..29495
                     /gene="argC"
                     /locus_tag="ACHUZQ_000928"
     CDS             28491..29495
                     /gene="argC"
                     /locus_tag="ACHUZQ_000928"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1024696.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29503..30279
                     /gene="argB"
                     /locus_tag="ACHUZQ_000929"
     CDS             29503..30279
                     /gene="argB"
                     /locus_tag="ACHUZQ_000929"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1024697.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30340..31713
                     /gene="argH"
                     /locus_tag="ACHUZQ_000930"
     CDS             30340..31713
                     /gene="argH"
                     /locus_tag="ACHUZQ_000930"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1024698.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31980..32897
                     /gene="oxyR"
                     /locus_tag="ACHUZQ_000931"
     CDS             31980..32897
                     /gene="oxyR"
                     /locus_tag="ACHUZQ_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1024699.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32880..34280)
                     /gene="sthA"
                     /locus_tag="ACHUZQ_000932"
     CDS             complement(32880..34280)
                     /gene="sthA"
                     /locus_tag="ACHUZQ_000932"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1024700.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34601..35251
                     /gene="fabR"
                     /locus_tag="ACHUZQ_000933"
     CDS             34601..35251
                     /gene="fabR"
                     /locus_tag="ACHUZQ_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1024701.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35251..35610
                     /locus_tag="ACHUZQ_000934"
     CDS             35251..35610
                     /locus_tag="ACHUZQ_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1024702.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35650..36750)
                     /gene="trmA"
                     /locus_tag="ACHUZQ_000935"
     CDS             complement(35650..36750)
                     /gene="trmA"
                     /locus_tag="ACHUZQ_000935"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1024703.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36864..37054
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37119..38963
                     /gene="btuB"
                     /locus_tag="ACHUZQ_000936"
     CDS             37119..38963
                     /gene="btuB"
                     /locus_tag="ACHUZQ_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1024704.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38908..39765
                     /gene="murI"
                     /locus_tag="ACHUZQ_000937"
     CDS             38908..39765
                     /gene="murI"
                     /locus_tag="ACHUZQ_000937"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1024705.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9130 a        10046 c        11021 g         9767 t
ORIGIN      
        1 aagcccaaca tcaacccaca atgacataat ggggggcggg ggaatgtcat atgcctatga
       61 atatgatgta aaatataata tatgtcattt gagaaaaaag agcagaatga ggtaaaaatc
      121 tttggctgtg tattcagtct ggtaatcatc caaggaaagc ttatcagaag ggtgatgact
      181 ccaattagta gaacatatat tttcgataaa agcaccgatg actttttcat gaagttctac
      241 agaacgcgag aaacagatgg ttctgcgggc cagacgtttg attcgggttc tcagggtcag
      301 attgttacgc tcaatgtgct gagtaaaaat cttaccagtc agatgcttct ccttcggtaa
      361 ctctctagcg taactacccc attcgtcagt ggtcaacatg ccgatggtaa aagggtaagc
      421 agcgcccgca attcacgaca ggtttcgtca gtccgggggc caaaagtgta ggccagaaca
      481 ccaccagttt tcgtgttgta cacgtaccag agccagtgct gtcgcgcttt actgccgaca
      541 aagctccatt gctgactaag tttgcagatg agtgccacat cagcatgagc gaccggggat
      601 gacgttattc ttcgtggcga gagtttttta aagttcggat gactgtatta atgccgattt
      661 tcagcgtacg ggcagtatcg cgaaccccgg caccgttgaa agtcatttca gcgatttggt
      721 ctttaactcc cggtttacgg ggctcgtaac agtaactgag ctgaaatacc cggtgacagt
      781 cacggccgcg gaatcgctca tgtctttttg ggttctgccc gtgacggtaa acctgaacgg
      841 actggcaacg ggggcaatga aggttaactg tgaccatgaa acaaccttaa aagcgagtat
      901 tatatcctta attcaactaa ttggaggcat caccgtttac cggagcgaca gcttgcggaa
      961 aggtcgtgca actacaaagc aacaaacaga ctacggcggg aattattttt ttcatcatcc
     1021 attaccacga aaacagcggc cactctttgg cgactgtggt gagttgtggc gcgtcgaaaa
     1081 cggcttgtgg tgaaactacc gtttcctcaa gcggaatatt tttgccgtag cgttctttga
     1141 gcttagcctg gaccagcggg ttgctgagat cgaactcttt caacttctgc tgctcgccca
     1201 cgcgcattcc cagcgcgttc tggtaaacct tccacaccac ttccgaacag tactggcgat
     1261 cgtcgctcca tgagaagctg aaatcatacg gtttaccgag ataacgtttt gccgtttgcg
     1321 ccagtttttg ctgttgttca acactcagtc cgccttcaac gcggcgaaca acgtatttgc
     1381 ccttttcacc atgggcgatc cactgcttga gcggggtgta tttcaccggg ccgaccgctt
     1441 caaaaacgta gggctttttg ttgcgtatca ccagcatacc ggtgtggcta taatcggaat
     1501 gggtcgccag ttggatcgct ttactttgcg atgagcgaga gatctgaaag atgatgtcgc
     1561 cggtttgtgg ctgccaggca aaggcaggta cagaaaccag caggctgagg atcagcagcc
     1621 tgtttttcat agttaaacgt ccatgtataa aaagcggtgg gtcgcagaca acgtgctcgt
     1681 tgtttatgcc ggatgcggcg tgaacgcctt atccggccta caagttcgtg caaattcaat
     1741 aaattgcaat atgacgtagg cctgataagc gtagcgcatc aggcgattcc actccgcgcc
     1801 gctctttttt gctttagtat tcccacgtct ccgggttaat ccccatctca cgcatgatct
     1861 cttttgccgc ttccgggatt tcgtcgctgc gctctttacg cagatcggca tcatccggca
     1921 aaggttgccc ggtaaaggca tgcagaaacg cttcgcacag cagctcgctg ttggtagcgt
     1981 gacgcaggtt gttcacctga cgacgcgtgc gttcatcggt gaggattttt aacaccttaa
     2041 gaggaatgga aaccgtaatc tttttgactt gttcactctt cttgccgtgc tcagcgtatg
     2101 ggctgatata ttcgccgctc cattcagcca tgagatactt aatcctcttc gtcaataaat
     2161 tgagaccaga ccacacaatt tatgtgggta ctgaccgtaa acccgcatag tttaccgtac
     2221 aggcgttacc gtgacatcgt gtaatgcacc tgtcggcgtg ataatgcata taattttaac
     2281 ggctatttgg gatttgctca atctatacgc aaagaagttt agatgtccag atgtattgac
     2341 gtccattaac acaatgttta ctctggtgcc tgacatttca ccgacaaagc ccaggaaact
     2401 tcatcacatg acgcgtaaac aggccaccat cgcagtgcgt agcgggttaa atgacgacga
     2461 acagtatggt tgcgttgtcc ctcccatcca tctttccagc acctataact ttaccggatt
     2521 taatgaaccg cgcgcgcatg attactcgcg tcgcggcaac ccaacgcgcg atgtggttca
     2581 gcgtgcgctg gcagaactgg aaggcggtgc tggcgcagta cttactaata ccggcatgtc
     2641 cgcgattcac ctggtaacga ccgtcttttt gaaacctggc gatctgctgg ttgcgccgca
     2701 cgactgctac ggcggtagct atcgcctgtt cgacagtctg gcgaaacgcg gttgctatcg
     2761 cgtgttgttt gttgatcaag gcgatgaaca ggcattacgg gcagcgctgg cagaaaaacc
     2821 caaactggta ctggtagaaa gcccaagtaa tccattgtta cgcgtcgtgg atattgcgaa
     2881 aatctgccat ctggcaaggg aagtcggggc ggtgagcgtg gtggataaca ccttcttaag
     2941 cccggcatta caaaatccgc tggcattagg tgccgatctg gtgttgcatt catgcacgaa
     3001 atatctgaac ggtcactctg acgtggtggc tggcgtggtg attgctaaag acccggacgt
     3061 tgtcactgaa cttgcctggt gggcaaacaa tattggcgtg acgggcggcg cgtttgacag
     3121 ctatctgctg ctacgcgggt tgcgaacgct ggtgccgcgt atggagctgg cgcagcgcaa
     3181 cgcgcaggcg attgtgaaat atctgcaaac ccagccgttg gtgaaaaaac tgtatcaccc
     3241 gtcgctgccg gaaaatcagg ggcatgaaat tgccgcgcgt cagcaaaaag gctttggcgc
     3301 aatgttgagt tttgaactgg atggcgatga gcagacgctg cgtcgtttcc tgagcgggct
     3361 gtcgttgttt acgctggcgg aatcattagg gggagtggaa agtttaatct ctcacgccgc
     3421 aaccatgaca catgcaggca tggcaccaga agcgcgtgct gccgtcggga tctccgagac
     3481 gctgctgcgt atctccaccg gtattgaaga tggcgaagat ttaattgccg acctggaaaa
     3541 tggcttccgg gctgcaaaca aggggtaaaa atgagtgtga ttgcgcaggc aggggcgaaa
     3601 ggtcgtcagc tgcataaatt tggtggcagt agtctggctg atgtgaagtg ttatttgcgt
     3661 gtcgcgggca ttatggcgga gtactctcag cctgacgata tgatggtggt ttccgccgcc
     3721 ggtagcacca ctaaccagtt gattaactgg ttgaaactaa gccagaccga tcgtctctct
     3781 gcgcatcagg ttcaacaaac gctgcgtcgc tatcagtgcg atctgattag cggtctgcta
     3841 cccgctgaag aagccgatag cctcattagc gcttttgtca gcgatctgga acgcctggcg
     3901 gcgctgctcg acagcggtat taacgacgca gtgtatgcgg aagtggtggg ccacggggaa
     3961 gtatggtcgg cacgcctgat gtctgcggta cttaatcaac aagggctgcc agcggcctgg
     4021 cttgatgccc gcgagttttt acgcgctgaa cgcgccgcac aaccgcaggt tgatgaaggg
     4081 ctttcttacc cgttgctgca acagctactg gtgcaacatc cgggcaaacg tctggtggtg
     4141 accgggttta tcagccgtaa caacgccggt gaaacggtgc tgctggggcg taacggttcc
     4201 gactattccg cgacacaaat cggtgcgctg gcgggtgttt ctcgtgtaac catctggagc
     4261 gacgtcgccg gggtatacag tgccgacccg cgtaaagtga aagatgcctg tctgctgccg
     4321 ttgctgcgtc tggatgaagc cagcgaactg gctcgtctgg cggctcccgt tcttcacgcc
     4381 cgtactttac agccggtttc tggcagcgaa atcgacctgc aactgcgctg tagctacacg
     4441 ccggatcaag gttccacgcg cattgaacgc gtgctggcct ccggtactgg tgcgcgtatt
     4501 gtcaccagcc acgatgatgt ctgtttgatt gagtttcagg tgcccgccag tcaggatttc
     4561 aaactggcgc ataaagagat cgaccaaatc ctgaaacgcg cgcaggtacg cccgctggcg
     4621 gttggcgtac ataacgatcg ccagttgctg caattttgct acacctcaga agtggccgac
     4681 agtgcgctga aaatcctcga cgaagcggga ttacctggcg aactgcgcct gcgtcagggg
     4741 ctggcgctgg tggcgatggt cggtgcaggc gtcacccgta acccgctgca ttgccaccgc
     4801 ttctggcagc aactgaaagg ccagccggtc gaatttacct ggcagtccga tgacggcatc
     4861 agcctggtgg cagtactgcg caccggcccg accgaaagcc tgattcaggg gctgcatcag
     4921 tccgtcttcc gcgcagaaaa acgcatcggc ctggtattgt tcggtaaggg caatatcggt
     4981 tcccgttggc tggaactgtt cgcccgtgag cagagcacgc tttcggcacg taccgacttt
     5041 gaatttgtgc tggcaggcgt ggtggacagc cgccgcagtc tgttgagcta tgacgggctg
     5101 gacgccagcc gcgcgttagc cttcttcaac gatgaagcgg ttgagcagga tgaagagtcg
     5161 ttgttcctgt ggatgcgcgc ccatccgtat gatgatttag tggtgctgga cgttaccgcc
     5221 agccagcagc ttgctgatca gtatcttgat ttcgccagcc acggtttcca cgtcatcagc
     5281 gccaacaaac tggcgggagc cagcgacagc aataaatatc gccagatcca cgacgccttc
     5341 gaaaaaaccg ggcgtcactg gctgtacaat gccaccgtcg gtgcgggctt accgatcaac
     5401 cacaccgtgc gcgatctgat cgacagcggc gatactattt tgtcgatcag cgggatcttc
     5461 tccggcacgc tctcctggtt gttcctgcaa ttcgacggta gcgtgccgtt taccgagctg
     5521 gtggatcagg cgtggcagca gggcttaacc gaacctgacc cgcgtgacga tctctctggc
     5581 aaagacgtga tgcgcaagct ggtgattctg gcgcgtgaag caggttacaa catcgaaccg
     5641 gatcaggtac gcgtggagtc gctggtgcct gcccattgcg aaggcggcag catcgaccat
     5701 ttctttgaaa atggcgatga acttaacgag cagatggtgc aacggctgga agcggcccgc
     5761 gaaatggggc tggtgctgcg ctacgtggcg cgtttcgatg ccaatggcaa agcgcgtgtg
     5821 ggcgtggaag cggtgcgtga agaccatccg ctggcatcac tgctgccgtg cgataacgtc
     5881 tttgccatcg aaagccgctg gtatcgcgat aatccgctgg tgatccgtgg gcctggcgct
     5941 gggcgcgacg tcaccgccgg ggcgattcag tcggatatca accggctggc acagttgttg
     6001 taatttcaga aatttaataa tgcccggtac tcatgttttc gggtttatgg tttctaatga
     6061 aatatattga attatcatag gattaggccg gattaagcgt ttacgacgaa tccggcaaga
     6121 agcaataagt acatggttag tttatatttg cagtccggtt tgctttgcat accggatttt
     6181 ctttttctta ccatcctgaa gttttttcat cttccctgat ttttcctcac catcattggt
     6241 catttttcgg ttgacgccct tcggcttttc cttcatcttt acatctggac gtctaaacgg
     6301 atagatgtgc acaacacaac atataactac aagcgattga tgaggtaagg tatgagcttt
     6361 tttcacgcca gccagcggga tgccctgaat cagagcctgg cagaagtcca ggggcagatt
     6421 aacgtttcgt tcgagttttt cccgccgcgt accagtgaaa tggagcagac cctgtggaac
     6481 tccatcgatc gccttagcag cctgaaaccg aagtttgtat cggtgaccta tggcgcgaac
     6541 tccggcgagc gcgaccgtac gcacagcatt attaaaggca ttaaagatcg cactggtctg
     6601 gaagcggcac cgcatcttac ttgcattgat gcgacgcccg acgagttgcg caccattgca
     6661 cgcgactact ggaataacgg tattcgtcat atcgtggcgc tgcgtggcga tctgccgccg
     6721 ggaagtggta agccagaaat gtatgcttct gacctggtga cgctattaaa agaagtggca
     6781 gatttcgata tctccgtggc ggcgtatccg gaagttcacc cggaagcaaa aagcgctcag
     6841 gcggatttgc ttaatctgaa acgcaaagtc gatgccggag ccaaccgcgc gattactcag
     6901 ttcttcttcg atgtcgaaag ctacctgcgt tttcgtgacc gctgtgtatc ggcgggcatt
     6961 gatgtggaaa ttattccggg aattttgccg gtatctaact ttaaacaggc gaagaaattt
     7021 gccgatatga ccaacgtgcg tattccggcg tggatggcgc aaatgttcga cggcctggat
     7081 gatgatgccg aaacccgcaa actggtgggc gcgaatattg ccatggatat ggtgaagatt
     7141 ttaagccgtg aaggagtgaa agatttccac ttctatacgc ttaaccgcgc tgaaatgagt
     7201 tacgcgattt gccatacgct gggtgttcga cctggtttat aaatagtgtg gcttttgtga
     7261 aaatcacaca gtgatcacaa attttaaaca gagcacaaaa tgctgcctcg aaatgagggc
     7321 gggaaaataa ggttgtcagc cttgttttct ccctcattac ttgaaggata tgaagctaaa
     7381 accctttttt ataaagcatt tgtccgaatt cggacataat caaaaaagct taattacgat
     7441 caatttgacc tacatctctt taaccaacaa tatgtaagat ctcaactatc gcatccgtgg
     7501 attaattcaa ttataacttc tctctaacgc tgtgtatcgt aacggtaaca ctgtagaggg
     7561 gagcacattg atgagcacgt cagacgatat ccataacacg acagccactg gcaaatgccc
     7621 gttccatcag ggcggtcacg accagagcgc gggggcgggc acaaccactc gcgactggtg
     7681 gccaaatcaa ctccgtgttg acctgttaaa ccaacattct aatcgttcta acccgctggg
     7741 tgaggacttt gactaccgca aagaattcag caaattagat tactacggcc tgaaaaaaga
     7801 tctgaaagcc ctgctgacag aatctcagcc gtggtggcca gccgactggg gcagctacgc
     7861 cggtctgttt attcgtatgg cctggcacgg cgcaggtact taccgttcaa tcgatggacg
     7921 cggcggcgcg ggtcgtggtc agcaacgttt tgcaccgctg aactcctggc cggataacgt
     7981 aagcctcgat aaagcgcgtc gcctgttgtg gccaatcaaa cagaaatatg gtcagaaaat
     8041 ctcctgggcc gacctgttta tcctcgcagg taacgtggcg ctggaaaact ccggcttccg
     8101 taccttcggt tttggtgccg gtcgtgaaga cgtctgggaa ccggatctgg atgttaactg
     8161 gggtgatgaa aaagcctggc tgactcaccg tcatccggaa gcgctggcga aagcaccgct
     8221 gggtgcaacc gagatgggcc tgatttacgt taacccggaa ggcccggatc acagcggcga
     8281 accgctttct gcggcagcag ctatccgcgc gaccttcggc aacatgggca tgaacgacga
     8341 agaaaccgtg gcgctgattg cgggtggtca tacgctgggt aaaacccacg gtgccggtcc
     8401 gacatcaaac gtaggtcctg atccagaagc tgcaccgatt gaagaacaag gtttaggctg
     8461 ggcgagcact tacggcagcg gcgttggcgc agatgccatt acctctggtc tggaagttgt
     8521 ctggacccag acgccgaccc agtggagcaa ctatttcttc gaaaacctgt tcaaatatga
     8581 gtgggtacag acccgcagtc cggctggcgc aatccagttc gaagcggtag acgcaccgga
     8641 aattatcccg gatccattcg atccgtcgaa gaaacgtaaa ccgacgatgc tggtgaccga
     8701 cctgacgctg cgttttgatc ctgagttcga gaagatctct cgtcgtttcc tcaacgatcc
     8761 gcaggcgttc aacgaagcct ttgcccgtgc ctggttcaaa ctgacgcaca gggatatggg
     8821 gccgaaatct cgctacatcg ggccggaagt gccgaaagaa gatctgatct ggcaagatcc
     8881 gctgccgcag ccgatctaca acccgaccga gcaggacatt atcgatctga aattcgcgat
     8941 tgcggattct ggtctgtctg ttagtgagcg ggtatcggtg gcctgggcat ctgcttctac
     9001 cttccgtggt ggcgacaaac gcggtggtgc caacggtgcg cgtctggcat taatgccgca
     9061 gcgcgactgg gatgtgaacg ccgcagccgt tcgtgctctg cctgttctgg agaaaatcca
     9121 gaaagagtct ggtaaagcct cgctggcgga tatcatcgtg ctggctggtg tggttggtgt
     9181 tgagaaagcc gcaagcgccg caggtttgag cattcatgta ccgtttgcgc cgggccgcgt
     9241 tgatgcgcgt caggatcaga ctgacattga gatgttcgag ctgctggagc caattgctga
     9301 cggtttccgt aactatcgcg ctcgtctgga tgtttccacc accgagtcac tgctgatcga
     9361 caaagcccag caactgaccc tgaccgcgcc ggaaatgacc gcgctggtgg gcgggatgcg
     9421 cgtactgggt gccaacttcg atggcagcaa aaacggcgtc ttcactgacc gcgttggcgt
     9481 attgagcaat gacttcttcg tgaacttgct ggatatgcgt tacgagtgga aagcgaccga
     9541 cgaatcgaaa gagctgtttg aaggccgtga ccgcgaaacc ggcgaagtga aatacactgc
     9601 cagccgtgcg gatctggtat ttggttctaa ctctgtcctg cgtgcggtgg ctgaagttta
     9661 cgccagcagc gatgctcacg agaagtttgt taaagacttc gtggcggcat gggtgaaagt
     9721 gatgaacctc gaccgtttcg acctgctgta atctgacctt cttcagcgac tgcctttcag
     9781 gcagtcgctg aagtttcttt accggcgtat agtgtccaca ggaaaactac acactggatc
     9841 tctcatgtct gccgcaggaa agagcaaccc actggcaatc agtggcctgg ttgtgctcac
     9901 acttatctgg agttatagct ggattttcat gaagcaagtc accagctaca tcggtgcctt
     9961 cgactttacc gccttacgct gcattttcgg cgctctcgtt ttattcatcg tccttttatt
    10021 acgtggtcgc ggaatgcgcc cgacaccgtt taaatacacc ttagccattg ccctgttaca
    10081 aacctgcggg atggttggcc tggcgcagtg ggcgctggtc agcggcggag cggggaaggt
    10141 ggcgatccta agctatacca tgccgttctg ggtggtgatt ttcgccgcgt tgtttctcgg
    10201 tgaacgcctg cgacgtgggc aatatttcgc gatactgatt gccgctttcg gcttattttt
    10261 ggtgttgcag ccgtggcaac tcgatttctc ttcgatgaaa agtgccatgc tggcaatcct
    10321 ctccggcgtc agttgggggg cgagcgcgat tgttgctaaa cgtctgtatg cccgtcatcc
    10381 gcgcgtggat ttattgtcgt taacatcctg gcagatgctc tatgcggcgc tggtgatgag
    10441 tgtggtcgct ttactggtgc cgcaacgtga aattgactgg cagcccaccg tgttctgggc
    10501 gctggcctac agtgcgattc tggcgacggc actggcgtgg agcttatggt tgtttgtatt
    10561 gaaaaacttg cctgccagta ttgccagctt aagcacactg gccgttcccg tttgcggcgt
    10621 actcttttcc tggtggctgc tcggcgagaa tccgggggcc gttgaaggta gcggtattgt
    10681 gctgattgtg atggcactgg cgctggtgag ccgtaagaaa aaagaagccg ttagtgtaaa
    10741 aaggatctga attttttctt catgtggggc gatcgcttat ttaacaaaat aacgataatg
    10801 ccccaccatg cgccagctaa acagcacatc ttcttcctgc gcacctgcgc caatgttatg
    10861 tatcaccagc ggcgtaccgt cgcgggcgaa gccatctgaa accaccccaa tatgtgccag
    10921 cccgttatct aatcgccagg agacaatatc gcccgcttga tagtcactgg ggttcttgct
    10981 gatggggcgt gttttatcgt ggtgggtaaa ccaggtttcc agattaggca cccgacggtg
    11041 atcgatgttg ctgtccgggc gctttaactg ccatttttgc gggtactcgg caaaattctt
    11101 cgccatatct tcgtgcacca gtttctgcaa atcgaccttc tggctgcgca atgcacggat
    11161 caccacatcg gaacataccc cgcgtttttg cgggacatca ccgccaggat aggtaagctg
    11221 catatacgcc ggatcgtaaa atagcgtgct gccgatttgc tgtctggcac cgtctgcgat
    11281 ggcaaggttg gtattggcct ggatttgtac cacggttggt ggaacggcgg gagattttaa
    11341 ggagtggctg gtaaatgccg ttagcaggct gagcagcgcc agtgaagctt tcatctggtt
    11401 cgtccgtgaa aataagagtc acagattcga ccttccggga taacgattgt tctgtagaga
    11461 aaatgtccag aagcggataa aaaactcctg aacaacgtgg gagtttcaga ttactggcac
    11521 agagtgccgg atgcggcgtg aacgccttat ctgtcctaaa aaacaagaaa attcaatata
    11581 ttgcagagat tgcgtaggcc tgataagcgt agcgcatcag gcaattttgc gttcaaactc
    11641 ccggacaagc cgggagtttg gagtaggtta ttcccactct tgcaggaaac gctgaccgta
    11701 ctggtcggct accagcagag cggcgtaaac ctgatctggc gtcgcgccgc caggcatgtt
    11761 gtggatggtt tcacattctg cacatgccgc ttcagccaca attcgcattt tcgccgggac
    11821 atcttcttta atatccagtt gagcgagagt tattggcaaa cctaccgcat ggctaagtgc
    11881 agctacggtt tcgatttcct caaccggtgc gttttccaga accagctgcg tcagcgtacc
    11941 aaatgccact ttttcaccgt gataatagtg atgcgcgtcc gggatagcgg tcaggccgtt
    12001 atgcactgcg tgcgccgcag ccagaccacc actttcaaaa ccaacaccgc tcaaataggt
    12061 gttcgcttca atcacgcgct ccagcgccgg agtcactaca tgctgttcgg cagcaagcat
    12121 cgctttttcg ccttcttcca gcagggtgtt gtagcacagt tcagccagtg ccagcgcagc
    12181 ctgggtgcac ttgccgcccg ccatggtggt cgcgccgcta cgagagcagg cacgcgcttc
    12241 aaaccaggtt gccagcgcat cgccgatacc cgccgctaac agacgtgcag gtgcgccagc
    12301 gacgattttg gtgtcgacaa tgaccatatt cgggttattt ggcaacagca gatagcggtc
    12361 aaactcaccc tcatcggtgt agataacaga caatgcgctg cacggtgcat cggtagaggc
    12421 gatagtcggt gcgatcgcta ccggaacacc catgaaatgt gccagtgctt tggctgtatc
    12481 gagggttttt ccgccaccga taccgagaat tgcgccacac tgcgcagtct ccgcgatgcc
    12541 acgcagacgg tcgatctcat tttgcgaaca tccaccgcca aacggcgcaa tttctactac
    12601 cagtccagca tctttaaagc ttttctcgac agtggattga gcaaaaccta aaacaaattt
    12661 gtcacccacc actaaccagc gttctgccag cggcttcagg tattcgccca gacgattaat
    12721 cacatcagcg ccctggatgt atttacccgg tgattgaata atgcggtcca taattgctcc
    12781 tttagagatg agtagtgcca aatgcggcat tccagtcgtg ttcgaacttc tctatagctg
    12841 actctaccgc aggggtgttg agcatttgtt gcgctacatc taagggcagg gtgatggatt
    12901 cacatcctgc cagtaaacag tccagcgcct gacgcggcgt tttaaagctg gctgccagca
    12961 ccatgctttc tggcgcgtgc atttctaaca gcgcttgcag ctcctgaacc gtacgaatgc
    13021 cgtctccgcc ctgggcatct acgcggttaa catacggagc aacgtatttt gcccctgcca
    13081 gcgcggccag taacccttgt gcagcactgt acactgcggt gccaagcgta gtaataccct
    13141 cttttttcag tattttaatt gctgccagac cttcggaagt caccgggatt ttcaccacaa
    13201 tacccggaat agcgtcgcgc aggcgcttcg cttcttccac catcccctgc gcgtcgcggc
    13261 tcatggtctg agcaaacaga atgccctcat caccaatcgc tttttgcaga cgcggcagca
    13321 cttcccatat ggactccttg ctggcagcga taatgctcgg gttagttgtc accccggcaa
    13381 tggggaatat gcgtgccaga cgttcgactt ctgcgacgtt agcggtgtcc agatacagtt
    13441 ccatgatgtt tccttttaca gttccagttc atgttgcagc aggctggcga tagcgtcggc
    13501 agaggcggcg ttaaccagcg cgttacggaa ttcttcgtgc atgatgcggc gagcgaggcg
    13561 cgagaaaatg cgcatgtgct gatcgcccgc agcgtgtttg ttcagggtta acatgatgat
    13621 gaattgcgct tcatcatcac cccagcgcac cggcgcttgc agacgcgcaa cgctgatggt
    13681 ggattgctca atgtgttctg atttgctgtg tggaatggca aaactaaagc ccagaccggt
    13741 agagaaaacg gcctcgcgcg cccacaagtc ggcttccagt ttacgcggat agcggcagcg
    13801 ccctgccagc agcaggttat cggtcatgcc tttgagcact tcttctttgc tgcgccagtc
    13861 gctttccagt gtgatgcact cggcggtgac cagcggtgcg tcttgttggg tcatgcggaa
    13921 ttgcgccagc aggtgctcca cttccagcga agtacggcag gccattgcct ggttgagcaa
    13981 cttgcggcac tcacggctat caagttgcgc catccgcgct ttcgccgccg gaattgatgg
    14041 tgcgctcatg ctgagttcat ccagccctaa gccgaccagc aacggcagca cggaaccttt
    14101 cgctcccagc tcaccgcaca gaccaatcca tttaccctgg cgatgcaccg cctgcacggc
    14161 gtaatcgagc gcccgcaaga atgccggatt caggctgttg tagtgacgag taaccttagc
    14221 gttatcgcga tccactgcca gcagatattg cgtcaggtca ttactaccaa tactaaagaa
    14281 atcaatctct tcgcagcatt gatcgatgat gaacatcacc gacggcactt ccagcataat
    14341 gccgagctgg atcttctcat caaacggaat gtgttcgtta cgtagttgct gtttggcttc
    14401 tgccagtttt tctttcaccc ataagatctc ttccattgag gagatcatcg ggatcatgat
    14461 tttcaggctg ccgtgagcgg aggcgcgcag gatcgagcgt agttgtgtgg taaataacga
    14521 cgcgtattct tcataaatac gcacggcgcg ataaccgagg aacgggtttg cctctgcggg
    14581 aatgtttaga taatcaacgg gtttgtcgcc gccaatgtcc atagtgcgca caataatgct
    14641 gcgtccgttg gcagactcca gcgcctgaca aaaaatgttg tacagctcgc tttcgcccgg
    14701 tgcactggtg cgatccatat agagcatttc agtgcggaac aaaccaacgc cttccgcacc
    14761 attgccgaat gcggcctgcg cttccacgga gtgagcgatg ttagcggcaa tttcaatgcg
    14821 gataccgtca gcggtacggg cttgttgggt cagccagaca cgctgttgct cacgcagggc
    14881 gtcctgtacg cgggcttctt gctgataata acgagctacg gcttctcctg gctcaaccac
    14941 aatcgccccg gcgttgccgt cgatataaat cgtttgatgc tgccacggag tgagggcatc
    15001 aatatccaca ccaaccagcg tcggaatgtt gaacgaacgg gcaaggatca ccgtatgtga
    15061 ggtggtgccg ccacttttca gcaacagacc tttgaggtga tttttatcca gttcgaggaa
    15121 ctggctgggg gtcagttcat cagccataca aatggcggac tgcgtcagtt tgcccggtgc
    15181 cgggaagcgt tgctcaccgt agatttgctg gagtaactgg aagcagacgt cgcgtacgtc
    15241 cagggcacgt tcttgcaggt agctgctgct ggaacgggcg aactcttcgc aaaagtgatt
    15301 cgcactggcg acaatcgctt cggcgcagct taatccctcg ctgacgcctg ccagtaagtg
    15361 ctgacgtaaa gatgtatcgc cagccaggga tcggtgagct tccagtatcg cgctggtagc
    15421 accgtcgcta tccagcagac gaaactcaat gtttttcagt accagcgtca ggccgttttc
    15481 cagtgtggat tgctcggcgt caacgtcttt ggctgcggga agattactca gcgcattgag
    15541 atctaaagaa gagatcggcg tcagaatgcc gcctgcacta ccgctgcaca cggtgcgggc
    15601 gcggataatt tgcggattca gattggtcag tgaaaccggc agtggttcca gttcgtcaga
    15661 tttaacttcc gccagcggcg catcgcagtg ggggaattca tcgcgcagcc attggcttaa
    15721 acgctggtgc gcttcctgtt cgtcggcccc ggaaatcagt aactggcagt tatcgcccgc
    15781 cagcgtatcg gtgccaatca gcgcaagggc gcttttggcg ttgcccttgc ggtcagtgtg
    15841 caggttatgc cactcaattt gtgatgaaaa agtattacac agcgtttcaa cgtggcttgc
    15901 cggacgcgca tgtacgccgt taggtagctc acaaataaat tccacaatca gggccattgc
    15961 ctctctcccg gtacggtttt ctgtttacag gataagagcg cacggcaacg gcctgccatg
    16021 tgacaaatct gccaaaagct ggacaaatgt aatgtaaccg tcaatttgcg acgcgtctca
    16081 caagacgccg ttttgcggca tgcttccggt ttatcgcaag ttatgaggcg gatcgcattt
    16141 ttgtactgat attacaaaag tccagtaaat ggccttttta tccactgttt gctccgcctg
    16201 cgattgccta ttgtttcctc catcaaaaat atggacatgg gctttcgcaa gcccgggagc
    16261 agcttatgaa tgagttggtg cagatcctga aaaatacccg tcagcattta atgacgggcg
    16321 tttcacacat gattcccttc gtggtatcgg gcggtatttt gctggcggtt tccgtcatgt
    16381 tgtatggcaa aggcgcagtg ccggatgccg tagccgatcc aaatctgaaa aaactgtttg
    16441 atatcggcgt tgcgggcttg acgctgatgg tgcctttcct cgccgcttac attggttatt
    16501 ccattgcaga acgttctgcg ctggctccat gcgctatcgg tacctgggtt ggtaacagct
    16561 ttggtgcggg cttctttggt gcactgatcg ccgggattat cggcggcatc gtggtgcatt
    16621 acctgaagaa aattccggtg cataaagttc tgcgctcggt gatgcctatc ttcattattc
    16681 ctatcgtcgg cacactgatt accgcaggca tcatgatgtg gggcttgggc gagcctgtag
    16741 gggcgttgac caacagcctg actcagtggc ttcaggggat gcagcagggc agcattgtta
    16801 tgctggcggt gatcatgggt ctgatgctgg cgttcgatat gggcggtccg gttaacaaag
    16861 tggcctatgc cttcatgctg atttgcgttg ctcagggtgt ttataccgtg gtggccatcg
    16921 ctgccgtggg tatttgtatc ccgccgctgg ggatggggct ggcgacgctg attggtcgta
    16981 aaaatttctc cgcagaagag cgcgaaaccg gcaaagcggc actggtgatg ggctgtgttg
    17041 gcgttactga aggggcgatt cctttcgccg ctgccgatcc gctgcgtgtc attccttcca
    17101 tcatggtcgg ttcagtttgt ggtgcagtaa ctgcggcgct ggtcggtgcg cagtgctatg
    17161 caggctgggg tggtctgatt gtgctgccgg tggttgaagg caagctgggt tatatcgcag
    17221 cagtggctgt cggagcagtg gtgacggctg tttgtgtgaa cgtgctgaaa agtctggcgc
    17281 gtaaaaatgg gtcttcgact gatgaaaaag aagacgacct ggatttggat tttgaaatta
    17341 attaattgag gaacgagcca tgacgaaaat tattgcagta accgcatgtc cttcaggtgt
    17401 tgcccatacc tatatggcag cagaggcgct ggaaagtgcc gcgaaagcga aaggctggga
    17461 agtgaaagta gaaacctagg ggtcaattgg tctggaaaat gaactgactg cggaagatgt
    17521 ggcgagcgct gatatggtga ttctgaccaa agatatcggc atcaagtttg aagagcgttt
    17581 tgctggcaag accatcgtgc gcgtcaacat cagcgatgca gtaaaacgtg ctgacgccat
    17641 tatgagcaaa attgaagctc acctggcgca aaccgcttaa gttcttctcc cgctcgcaag
    17701 ggcgggttcg ctttcccaca ggagttcctc atgacgaatc gtatctctcg cctcaaaact
    17761 gcactgtttg ccaatacccg tgaaatctcg ctggagcggg cgctgcttta taccgccagc
    17821 catcggcaaa ccgaaggcga accggtgata ttgcgccggg cgaaagcaac agcgtatatc
    17881 cttgaacatg ttgaaatttc gattcgtgat gaagaactga ttgccggtaa ccgcaccgta
    17941 aaaccgcgcg ccgggattat gtcgccggaa atggaccctt actggctgct gaaagagctg
    18001 gatcaattcc cgacgcgtcc gcaggaccgc tttgctatca gcgaagaaga taaacgtatc
    18061 taccgtgaag agttgttccc gtactgggaa aaacgttcga tgaaagattt catcaacggg
    18121 cagatgacag atgaagtaaa agccgcgacc aacacgcaga ttttcagcat caaccagacg
    18181 gataaaggcc aggggcacat tattattgat tacccacgcc tgttgaatca cggtctgggt
    18241 gagctggtgg cacagatgca gcaacattgt cagcaacagc cggagaatca cttttatcag
    18301 gccgcgttgt tactgctgga agcctcgcag aaacacattt tgcgttacgc cgaactggcg
    18361 gaaacgatgg cggcaaactg cacagatgca cagcgtcgcg aagaactgct gactattgcg
    18421 gagatctctc gccataacgc cgaacataag ccgcagacgt tctggcaggc gtgccagtta
    18481 ttctggtaca tgaacatcat tctgcaatac gaatccaacg ccagttcgct gtctttaggg
    18541 cgcttcgacc agtatatgtt gccgttctat caggcatctt taacccaggg cgaagatccg
    18601 gcgttcctga aagaactgct cgaatcttta tgggtgaaat gcaacgacat cgtgctgttg
    18661 cgctccacca gcagcgcgcg ttatttcgca ggtttcccga ccggctatac cgcactgctc
    18721 ggcgggttaa ccgagaacgg acgtagcgcg gtgaacgtgc tttcgttcct ttgccttgac
    18781 gcctatcaaa gcgtgcaatt accgcaaccg aacctcggcg tgcgcactaa cgccttgatc
    18841 gacacgccgt tcctgatgaa aaccgccgaa accattcgcc tcggcaccgg tattccgcaa
    18901 atctttaacg atgaagtggt ggtaccagcg ttcctcaacc gtggcgtttc gctggaagat
    18961 gcgcgcgact attccgtagt gggctgtgtg gaattatcta ttcccggcag aacctacggc
    19021 ttgcatgaca tcgcgatgtt taatctgctg aaagtgatgg aaatctgcct gcatgaaaat
    19081 gaaggcaatg ctgcgctgac ttatgaaggt ttactggagc agatccgcgc caagatcagc
    19141 cactacatca ccctgatggt tgagggcagc aatatttgtg atatcggcca tcgcgactgg
    19201 gcacctgtac cgctgctctc gtcttttatc agcgattgtc tggaaaaagg tcgcgatatt
    19261 accgatggcg gcgcgcgtta taacttctcc ggcgtacagg ggatcggtat cgccaacctg
    19321 agcgattctc tccatgcgtt gaaagggatg gtttttgagc aacagcgttt aagttttgac
    19381 gaattgctgt cggtattaaa agccaacttt gcaacgccag aaggcgaaaa agtccgtgct
    19441 cgcttaatta accgcttcga gaaatacggt aacgatatcg acgaggtgga taacatcagc
    19501 gccgaactgt tacgccacta ctgcaaagaa gtggaaaaat accagaaccc gcgcgcggtt
    19561 acttcacgcc gggatcgtat accgtttctg ctcacgtccc gttgggatcg gtggttggcg
    19621 cgacgccaga cggtcgtttt gccggggaac aactggcaga cggcggcttg tcacctatgc
    19681 tgggccagga cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag ctcgataata
    19741 cgctgctgtc taacggtacg ttgctgaacg tgaaattcac tccggcgacc ctggaaggtg
    19801 aagcgggatt acgcaaactg gccgacttct tacgggcgtt tacccagctt aagttgcagc
    19861 atattcagtt taacgtggtg aacgccgaca cgttgcggga agcgcaacag cgcccacaag
    19921 attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc gaactgtcga
    19981 aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt tgtggaaacg
    20041 cgccgcgatg atgtggcgcg cattttcaac attcagcgtt attcactgaa tgacggcgag
    20101 ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccgtg gtgtgctaat
    20161 ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg tctgcactgt
    20221 gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg gattggtcgc
    20281 gatatcagcc ttgacgctct ggaacgggaa gtgatgaaag atgacatttt ctttcgcacg
    20341 tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga gtttgctacc
    20401 cgttttttac agcgactgcg gctgtggggt gtgtcatgcg ccattgaaac tgccggagac
    20461 gcgccagcaa gcaagctgtt accgctggcg aaattgtgcg atgaagtgct gttcgattta
    20521 aaaattatgg acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc acgcgtgctg
    20581 gagaatctgc gtttgctggt gagtgagggt gtcaacgtga tcccgcgttt accgttgatc
    20641 cctggtttca cgctcagcag ggagaatatg cagcaggcgc tggatgtact gatcccgctg
    20701 aaaatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa ataccgcctg
    20761 ctggggaaaa catggtcgat gaaagaggtg cctgcgccgt cgtcagccga tgtgacaacg
    20821 atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta aaatggcata
    20881 cctggtggca gtaaccgcct gcgtaagcgg cgtggcgcat acttatatgg cggcggaacg
    20941 gctggaaaag ttgtgccagt tagagaagtg gggcgtcagc attgaaactc agggcgcgct
    21001 gggaacggag aatcgtttag cggacgagga tatccgtcgg gcggatgttg ctctgttgat
    21061 tacggatatc gagcttgccg gtgccgagcg atttgaacat tgccgctatg tgcaatgcag
    21121 catctacgca ttcctgcgtg agccgcagcg ggtaatgagc gcggtgcgca aagtgctttc
    21181 tgcgccgcag caaacccatc ttattctgga gtagtcggtt tttctgtcag ttggctgtgg
    21241 tactgccggc gatattccga cggcgagcgt tcggtgtttt tacgaaacag acggcagaag
    21301 tagttgctgt cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag gtcatagcct
    21361 ttcaataacg tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt aaaaccaatg
    21421 gcccccgttt tttgaaacag gtgcgagaga tagtttggtg aaatatagaa tgcctgtgcg
    21481 acagattcgc gggtaagcgc ggaggcatag cgttcgtcga tataatcgcg aatagcttca
    21541 aatagtgcct ggctgcgtga ggcggtctgg atttggctgc cgagcaaatc gcggcagtgg
    21601 ctaagcaggc tggctacgat aagccttgcc gtttgctgct cctgcggctg catttgcatt
    21661 tcattgagcg tttgtagcag aaaagaacct atacgtgggc cgcgtcgggc gacgtgttgc
    21721 ttcgccagat tttgatattg tttgccatcc cattgcacga cgctgaagcc gagttgctgt
    21781 ttgccaaaca gcacgctaaa ggtggtagcg ggtgttttcc attgtgggaa attccagcca
    21841 ccagcggcaa cgtacagcac atcgccggga actaacgttg ctccagcgcc ggtatcaaca
    21901 aactcacctt ccagcacaat ttccagccgt ggaaaatcga cctgatacgc aagatcgggc
    21961 accgggccgt tactgctggc aaagtaaatc tggcgcaggg acagcgggcc gttgatcagg
    22021 cgggagagca gatagctgac gtcgtgatac atgttattcc cttaggtatg tagacgggaa
    22081 aacagtaact ggcattatta aaaaaggcca cctgaaaagt gtggcctgag gggagttcga
    22141 tgtaaacatt gcggcagttg tattgccgga tgtggcgtaa acgccttatc cagcctaccg
    22201 ccgcgatctg taggccggat aagacgcgtc aagcgtcgca tccggcaaat agcgcctggc
    22261 tgataattac tgattaccta cctggtcgcc atacggcaga gtgtcgtaat cgatcagcgc
    22321 gtttttcttg tacgggttac caatccagcg ggtagtttct ttgaactgcg gattcaccac
    22381 tgaacgggtt ggatcgtaac cgtcgtaaga taagcccgcc aaatctgacc aggtgtggat
    22441 cagttccgcc aggctgtatt tacgatcgac atcctgtgag aaatcacggg gatgagtcgc
    22501 ttgccatttt tccgacgtcc acagcaggaa cggaatggtg tacatgtggc gcgtcgggtt
    22561 gtcttcgtta cgcccctgag ttttgtgcgg cggcgtgtcg taaacctctt caccgtggtc
    22621 agagaagtaa accaggaatc cgttcgggtc tgccgcttta aagtctttaa tcaggctggc
    22681 aaccacatga tcgttataca ggttagcgtt gtcataatcg ttatatgatt ccagctcttc
    22741 tgcgcttaat cctggcggaa catgatcggt attgccatca aacttgccct gattttccgg
    22801 gtagcggtat ttgtatttga tatgcgtacc cagcagatga acgatgatca gtttcttcgg
    22861 cgcagggtca ttcagcacat cctggaacgg cttcagcacg ttggtgtcgt attcacgcgc
    22921 actctgcgta cgttgctggt tcatgtagta ctgcttgtcg gtctggcgcg aaaataccgt
    22981 cagcatggta ttgcgggcgg tcatcgtctg ctggttggtg atccagaagg ttttataacc
    23041 cgcctgtttc atcatgttca tcagcgacgg ctgcgtcaga tacagatccg ggttcttttc
    23101 attggcaaag gtcagcgcct gttgcaggat ttcaatggtg tacggacgag acgtaactac
    23161 gttattaaac acggtcagat tcggatcggt tttatgcagc gcatccagct ccggcgtggt
    23221 ttcacgcgga taaccgtaca gactcatgcg tccgcgctgg gtcgactcgc caatcaccag
    23281 cactaaagtg cgcggttcgt tacccgattc atctttgaaa ttagccagtg gcggcaaggc
    23341 attattttca ttcagtaact ttgttagcga gtttagttgc tgacgatact gataatagcc
    23401 ggtcaggaat tgccacggtg cggcaggctc catacgcgag gccaggttat ccaacgtttt
    23461 ctcaaacggc ttgtttttga taaacgtatt catggcgatc ggatgcagaa tcaagccata
    23521 aagcagggca aaagagacaa cataacgcca cggctttgga atatagaccg ggcgcaggcg
    23581 tgtccacagc agaactgcca ccgccgtata ggccagcgcg ataagcacaa ttttcaggct
    23641 gaaatactgg cttaaatact cgctggcttc gttggtgttg gtttcgaaca tcacaaacag
    23701 aacgctctgc gagaactcct gcccgtagat gacgtagtag catagcgccg ccagagaggc
    23761 cgcccatagc accacgccga ttactgcggc aataatttta atccgcttcg gaaagaggaa
    23821 taccgggatc aaccacagcg agctgaataa cagcgagtcg cgaatgccgt tggtgccact
    23881 ataaccactg atgtaaataa tggcctgtag cagagtagag aaaaaccaaa agtagagcag
    23941 tgcccaaccc agggctttcc agctaaaaag gggtttagcc tggacttctg tggaatgcat
    24001 agtaagaacc tgtcttgaaa aaaatatcgc cgaatgtaac gacaattcct taaggatatc
    24061 tgaaggtata ttcagaattt gaataaaatg cagacagaaa tatattgaaa acgagggtgt
    24121 tagaacagaa gtatttcaga aaaccctcgc gcaaaagcac gagggtttgc agaagaggaa
    24181 gattagccgg tattacgcat acctgccgca atcccggcaa tagtgaccat taacgcttgt
    24241 tcgacgcgag gatccggttc ctggccttct ttttctgcct ggcgggagcg gtgcagcaac
    24301 tcggcctgca atacgttcag cgggtcggtg taaatattcc gtagctgaat agactctgca
    24361 atccacggca gatcggccat cagatgggaa tcgttggcaa tcgccagcac cactttgatg
    24421 tcttcttctt gcaggttgcg taactcttta cctaacggcc acagtgcttt gtctaccaga
    24481 cgttgatcat agtattccgc caaccacagg tctgctttgg cgaagaccat ctccagcatg
    24541 ccgagacgcg tcgagaagaa tggccaatcg cggcacatgg cttccagctc gctctgtttg
    24601 ccatcttcga ccactttttg cagcgccgta cctgcaccca gccaggcggg gagcatcagg
    24661 cggttttgcg tccaggcaaa aatccacggt atggcgcgca gcgactcgac gccgccggtt
    24721 gggcgacgtt tcgccggacg tgaacccaac ggcagtttgc ccaattcttg ttccggcgta
    24781 gcggagcgga agtaaggcac aaaatctttg ttttcacgta cgtagccacg gtagagatcg
    24841 caggagatga ctgacagttc atccataatg cgacgccagc tctctttcgg ctcaggtggt
    24901 ggcagcaggt tggcttccag aatcgccccg gtgtaaagcg acaggctgct gacggtgatt
    24961 tctggcagac catatttaaa gcggatcatc tcgccctgtt cagtcacgcg caggccacct
    25021 ttcaggcttc ctggcggttg tgacagcagc gccgcatggg caggtgcgcc gccgcgacca
    25081 atggaaccgc cgcgaccgtg gaacaacgtc agctcaatac ccgctttttc gcaggttttg
    25141 attaatgcat cctgtgcctg atattgcgcc caggaagctg ccatcacgcc cgcatctttt
    25201 gctgagtcgg aatagccaat catcaccatc tgtttgccct gaatcaggcc gcgataccag
    25261 tcgatattga gcagctgggt catgacatcg ttggcgttgt tcaggtcatc gagggtttca
    25321 aacagcggag caaccggcat cgcaaacccg atacccgctt ctttcagcag caggtggaca
    25381 gccagtacgt cggacggcgt tttcgccatc gagatcacgt aggcggcaat ggagccttgc
    25441 ggcgcttcgg caatcacctg gcaggtatcg agcacttcgc gcgtttcggc gcttggttgc
    25501 cagttgcgcg gcagaagcgg acgtttggag ttcagttcgc ggatcaggaa cgcctgtttg
    25561 tcggcctctg accagctttc gtagtcgccg ataccgaggt agcgggtcag ctcgcccagc
    25621 gcttcggtat ggcgggtgct ctcctgacgg atatcaatac ggaccagcgg tacgccgaaa
    25681 catttcacgc ggcgcagggt gtcgagcaga tcgccgttgg cgataatacc catgccacac
    25741 gcctgaagtg actggtagca agcgtagagc ggttcccaca gttcttcgtt ttgtgtcagc
    25801 agcccttctg gttttggcag ttcttcgcct ttcaggcgcg cttccagcca tgcctgtgtc
    25861 gccatcaggc gagaacgcag gtttttcatc agatagcgat acggttctgc ggcaccttct
    25921 tcgccaacca gcgccagcag ttcaggggtc gcttcaacca tcgacagttc agaaaccaga
    25981 acctgaatat ctttcaggaa cagatcggtg gctttccagc gactgagtag caggacgtgg
    26041 cgggtgatat cggcagtgac gttcgggttg ccgtcgcggt caccgcccat ccacgaagta
    26101 aaacggaccg gaacaaattc gacgggcagt ttgtagccga ggttctcttc cagttgttcg
    26161 ttcagttcgc gcaagtaatt cggtacgcct tgccacaggc tgttttctac tacggcaaag
    26221 ccccatttgg cttcatctac cgggcttgga cgcagcttac ggatttcatc agtatgccat
    26281 gactgggcga ttaactggcg caggcgacgc atcagctggt tgtgttcgta gtcagcgata
    26341 tctttgttat cgagctgttt taagcaggcg ttcacttcca ccattttgtg gatcagtgta
    26401 cgacgggtaa tttcggttgg gtgagccgtg aggaccagtt ccagcgacag cgattccact
    26461 gcttttttga tggtgtcttc gctcagttcc ggctggtttt tcagtttgcg cagggtgcgg
    26521 gcgatcactt ccgggttgct ggcagcttcg cctttcggcg aaatgctgtg gtattgctcg
    26581 gcggtgttgg ctaggttcag gaactgacta aacgcacgcg caacgggcag cagctcgtcg
    26641 ttcgacaaat tttgtaaggt ggtgagcaac tcctggcggt tagcatcatt gccagcgcgt
    26701 gaagatttcg acaacttacg gatagtttct acgcgttcaa gaatgtgttc tcccaacgca
    26761 tccttgatgg tttctcccag cactttgccg agcatactga cattactacg caatgcggaa
    26821 tattgttcgt tcatattacc ccagacaccc catcttatcg tttgatagcc ctgtatcctt
    26881 cacgtcgcat tggcgcgaat atgctcgggc tttgcttttc gtcgtctttt ataaagccac
    26941 gtaaaagcgg tgacgtcaaa tgctgcgaaa tcgcttcagc aaacgaataa atagcaggaa
    27001 tttacgccat taaattcacg acgctttaaa taagcgtaac ttatggaaat gttaaaaaat
    27061 cgccatacat aacaccaaag gtgtaggtcg gataagatgc gcaagcatcg catccgacat
    27121 tattgcggca ccggagtttg gcaacggtgc cggatgcggc gcgagcgcct tatccggcct
    27181 acgttttaat gccagcaaaa atggtgaatt acctggataa tcaactcacg ggtgggttta
    27241 ataaaccgcg tctccagata ttcatcaggc tgatgagctt gattgatgga acccggaccg
    27301 agcaccagcg tcgggcataa cgtttgaata aacggcgctt cggtacagta attcaccact
    27361 tcggtttttg ctccgagcaa tttctcaacc acttcaacca gttgatgatt cggtgggcat
    27421 tcatagccag ggatcggcgg atgcagctcg tcgaccgtca gacgacccgg ccagcgttcg
    27481 ctcaccggag ccaatgcatc gttgagcaaa ccattaagtt cattgagtgt catgccaggc
    27541 agcggacgaa tatccatatg caactcacag caagcgcaaa tacggttaga agcgtcgcca
    27601 ccgtgaatat gcccgaggtt gagcgtaggg tatggcacgg taaatgcttc gtagtgataa
    27661 cgttctttca ggttatcgcg caattgcaaa atatgcccga tggcgtcgtg cattagttcg
    27721 atagcgttaa ctccgcgtgc tggatcgctg gagtgccccg actggccctg aatacggatg
    27781 gcgttagaga tatgaccttt atgtgcgcgt accggttgta gtgacgtcgg ttcgccaatg
    27841 atggcgcaat ccgggcgcag ggcggtagtt tcggcaaaat aacgcgctcc ggccatactg
    27901 gtttcttcat cagcagtcgc cagaatgtag agcggttttt tcagtttcgt gacgtcgaca
    27961 tcgcgtagcg tatcaaggat aaacgcaaaa aagcctttca tgtcggcggt gcctaagccg
    28021 taaagcttgc cgtcatgctc cgtcagtgta aacggatcgc gcgtccagcg accgtcatca
    28081 aatggcaccg tatcggtatg ccccgccagc aacaagccgc cagccccctg tccgatactt
    28141 gccagcatat tgaatttgtt gcgagttcct ggcacaggct gcacttccac attgaagcct
    28201 aaatctttaa accagtccgc cagcagagtg attaaatctg cattgctttg atcgagtgcc
    28261 tcttccgtgg cgcttattga aggtgtggca atcagagcac ggtaaatctc gataaatggc
    28321 ggtaatttgt ttttcattgt tgacacacct ctggtcatga tagtatcaat attcatgcag
    28381 tatttatgaa taaaaataca ctaacgttga gcgtaataaa acccaccagc cgtaaggtga
    28441 atgttttacg tttaacctgg caaccagaca taagaaggtg aatagccccg atgttgaata
    28501 cgctgattgt gggtgccagc ggctacgctg gcgcagagct agtgacctat gtaaatcgcc
    28561 atccgcatat gaacataacc gctttgactg tttcagcgca aagcaatgat gcgggaaagt
    28621 taatctccga tttgcatccg cagctaaaag gcatcgttga gctgccgttg cagccgatgt
    28681 cggatatcag cgagtttagc ccaggggtgg acgtagtgtt tctcgccacc gcccatgaag
    28741 ttagccacga tttagcgccg cagtttcttg aagcgggctg cgtggtgttc gacctttccg
    28801 gtgcgtttcg tgttaacgac gccaccttct atgaaaaata ttacggcttt acccatcaat
    28861 acccggaact gctggaacag gcagcctacg gtctggcgga gtggtgcggt aataaattaa
    28921 aagaagcgaa tttgattgcg gtgccaggct gttatccgac ggcggcacag ctggcgctga
    28981 aaccgttgat tgatgccgat cttcttgacc tcaatcagtg gccggtgatc aacgccacca
    29041 gcggcgtgag cggtgcagga cgtaaggcag cgatttcaaa tagtttttgt gaagttagcc
    29101 tgcaaccgta tggcgtcttt actcatcgcc atcaaccaga gatcgccaca cacctcggtg
    29161 ctgacgttat cttcacccca catctgggca atttcccgcg cggcattctc gaaaccatta
    29221 cctgccgcct gaaatcgggt gtgacccagg cgcaagtcgc gcaagtgtta cagcaggcgt
    29281 atgcccataa accgctggtg cggctgtatg acaaaggcgt tccggcgctg aaaaatgtcg
    29341 ttgggctgcc attttgcgat atcgggtttg ccgttcaggg cgagcatctg attattgtgg
    29401 cgaccgaaga caacttactg aaaggcgcgg cggcacaagc ggtacagtgc gccaatattc
    29461 gtttcggcta tgcggaaacg cagtctctta tttaagggtg caatgatgaa tccattaatt
    29521 atcaaactgg gcggcgtact gctggatagt gaagaggcgc tggaacgtct gtttagcaca
    29581 ctggtgaatt atcgtgagtc acatcagcgt ccgctggtga ttgtgcacgg cggcggttgc
    29641 gtggtggatg agctgatgaa agggctgaat ctgccggtga aaaagaaaaa cggcctgcgg
    29701 gtgacgcctg ctgatcagat agacattatc accggagcac tggcgggaac ggcaaataaa
    29761 accctgttgg catgggcgaa gaaacatcag attgcggccg taggtttgtt tctcggtgac
    29821 ggcgacagcg tcaaagtgac ccagcttgat gaagagttag gtcatgttgg actggcgcag
    29881 ccaggttcgc ctaagcttat caactccttg ctggagaacg gttatctgcc ggtggtcagc
    29941 tccattggcg taacagacga agggcaactg atgaacgtca atgccgacca ggcggcaacg
    30001 gcgctggcgg caacgctggg cgcggatctg attttgctct ccgacgtcag cggcattctc
    30061 gacggcaaag ggcaacgcat tgccgaaatg accgccgcga aagcagaaca actgattgag
    30121 cagggcatta ttactgacgg catgatagtg aaagtgaacg cggcgctgga tgcggcccgc
    30181 acgctgggcc gtccggtaga tatcgcctcc tggcgtcatg cggagcagct tccggcactg
    30241 tttaacggta tgccgatggg tacgcggatt ttagcttaag ttttgttggc cggaggcgca
    30301 gctttccggc attgaatttc aaaataagga aacagagtta tggcactttg gggcgggcgt
    30361 tttacccagg cagcagatca acggttcaaa caattcaacg actcactgcg ctttgattac
    30421 cgtctggcgg agcaggatat tgttggctct gtggcctggt ccaaagccct ggtcacggta
    30481 ggcgtgttaa ccgcagaaga gcaggcgcaa ctggaagagg cgctgaacgt gctgctggaa
    30541 gatgttcgcg ccaggccaca acaaatcctt gaaagcgacg ccgaagatat ccatagctgg
    30601 gtggaaggca aactgatcga caaagtgggc cagttaggca aaaagctgca taccgggcgt
    30661 agtcgtaacg atcaggtagc gactgacctg aaactgtggt gcaaagatac cgttagcgag
    30721 ttactgacgg ctaaccgaca gctgcaatcg gcgctggtgg aaaccgcaca aaacaatcag
    30781 gacgcggtca tgccaggtta cactcacctg caacgcgccc agccggtgac gttcgcgcac
    30841 tggtgcctgg cctatgttga gatgctggcg cgtgatgaaa gccgtttgca ggatgcgctt
    30901 aagcgtctgg atgtcagccc attaggctgt ggcgcgctgg cgggaacggc ctatgaaatc
    30961 gaccgtgaac agttagcagg ctggctgggc tttgcttcgg cgacccgtaa cagtctggac
    31021 agcgtttctg accgtgacca tgtgttggaa ctgctgtcgg ctgccgctat cggcatggtg
    31081 catctgtcgc gttttgctga agatctgatt ttctttaaca ccggcgaagc ggggtttgtg
    31141 gagctttctg accgcgtgac ttccggttca tcattaatgc cgcagaagaa aaacccggat
    31201 gcgctggagc tgattcgcgg taaatgcggt cgggtgcagg gtgcgttaac cggcatgatg
    31261 atgacgctga aaggtttgcc gctggcttac aacaaagata tgcaggaaga caaagaaggt
    31321 ctgttcgacg cgctcgatac ctggctggac tgcctgcata tggcggcgct ggtgctggac
    31381 ggcattcagg tgaaacgtcc acgttgccag gaagcggctc agcagggtta cgccaacgcc
    31441 accgaactgg cggattacct ggtggcgaaa ggcgtacctt tccgcgaggc gcaccatatc
    31501 gtgggtgaag cggtggtgga agccattcgt cagggcaaac cgctggaaga tctgccgctc
    31561 gacgagttgc agaaattcag cccggtgatt ggcgaagatg tctatccgat tttgtcgctg
    31621 caatcgtgcc tcgacaagcg tgcggcaaaa ggcggcgtct caccgcagca ggtggcccaa
    31681 gcgattgctt ttgcgcaggc gcggttgggg taagaacatt tatatgtaca aatttgagcc
    31741 tggcttatcg ccgggctttt ttatggcaaa aaaaagcgga tcctggagat ccgcaaaagt
    31801 tcacgttggc tttagttatt cgagttgaga aactctcgaa acgggcagtg acttcaaggg
    31861 ttaaaagagg tgccgctccg tttctgtgag caattatcag tcagaatgct tgatagggat
    31921 aatcgttcat tgctattcta cctatcgcca tgaactatcg tggcgatgga ggatggataa
    31981 tgaatattcg tgatcttgag tacctggtgg cattggctga acaccgccat tttcggcgtg
    32041 cggcagattc ctgccacgtt agccagccga cgcttagcgg gcaaattcgt aagctggaag
    32101 atgagctggg cgtgatgttg ctggagcgga ccagccgtaa agtgttgttc acccaggcgg
    32161 gaatgctgct ggtggatcag gcgcgtaccg tgctgcgtga ggtgaaagtc cttaaagaga
    32221 tggcaagcca gcagggcgag acgatgtccg gaccgctgca tattggtttg attcccacgg
    32281 ttggaccgta cctgctaccg catattatcc cgatgctgca ccagaccttt ccaaagctgg
    32341 aaatgtatct gcatgaagca cagacccacc agttactggc gcaactggac agtggcaaac
    32401 tcgattgcgt gatcctcgcg ctggtgaaag agagcgaagc attcattgaa gtgccgttgt
    32461 ttgatgagcc aatgttgctg gctatctatg aagatcaccc gtgggcgaac cgcgaatgcg
    32521 taccgatggc cgatctggca ggggaaaaac tgctgatgct ggaagatggt cactgtttgc
    32581 gcgatcaggc aatgggtttc tgttttgaag ccggggcgga tgaagataca cacttccgcg
    32641 cgaccagcct ggagacgctg cgcaacatgg tggcggcagg tagcgggatc actttactgc
    32701 cagcgctggc tgtgccgccg gagcgcaaac gcgatggggt tgtttatctg ccgtgcatta
    32761 agccggaacc acgccgcact attggcctgg tttatcgtcc tggctcaccg ctgcgcagcc
    32821 gctatgagca gctggcagag gccatccgag caagaatgga tggctatttc gataaagtgt
    32881 taaaacaggc ggtttaaacc gtttaacgca gctacccgat aggcttccgc catggtcggg
    32941 tagttaaagg tggtgttgac gaagtactca atagtgttgc cgccaccttt ctgttccata
    33001 atcgcctgac cgatatgaat aatttcggca gcgcgctcgc caaagcagtg aatacccaga
    33061 atctcttttg tttcccgatg gaacaaaatt ttcagcgtgc ccacgttcat gccgacgatt
    33121 tgtgcgcgtg ccagatgttt aaactgggcg cggcccactt catatggcac tttcatcgcg
    33181 gtcagctgct gttcggtttt gcccacagag ctgatttccg ggatggtgta aatgccggta
    33241 gggatatctt caatcagatg cgcggtggct tcgcctttta ccagcgcctg cgcggcaatg
    33301 cgcccctggt cataggccgc tgatgccagg ctcggataac caatcacgtc gcccaccgcg
    33361 taaacgtgcg gctgtgcggt ctgatacatg ctgttgacct tcagctgtcc gcgactgtca
    33421 gtttccagcc caatgttctg taacgccagc gaatcggtat taccggtacg accgttggca
    33481 tagagcaggc agtcagcttt cagtttttta cccgacttca gatgcatgat cacaccgtcg
    33541 tcacagcctt cgatcttctc gtactcttcg ttgtgacgaa tcactacgcc actgttccag
    33601 aagtgatagg agagagaatc tgacatctct tgatcgagaa atgccagcag gcgatcgcgg
    33661 gtgttgatca gatccacttt tacatccata ccgcggaaga tcgacgcata ttcacagccg
    33721 atcactccag caccatagat aagtacatgg cgcggttcgt ggtgcatgct aagaattgag
    33781 tcgctgtcgt aaatgcgtgg atgggtgaaa tcaacatctg ttggatgata tggacgagag
    33841 ccgcaggcaa taacaaattt ttcagcggtt agtgtttcga cgctgccgtc cgggcaatcc
    33901 agcgccaacg tatgctcgtc aacaaagcga gcgttcccct gcaatatttc acagtgatta
    33961 cgttcgtaaa atccctgacg catgcgcgtt tgttgattaa tcacgttatc ggcatggtta
    34021 aggatatcgg caaaagaaga gcggagcagt cgcgaatggt cgctgtatag tgggttttga
    34081 ttgaattcta taatgcggct gacggcgtga cggagagctt tcgacgggat ggtgccccag
    34141 tgggtgcaac cgccgccaac attttgataa cgctcgataa ctgcgacgcg cacaccttgc
    34201 ttaaccaggc ccattgcagc gccttcgccg ccggggccgg aacctattac tatggcatcg
    34261 taatcgtagg aatgtggcat ggtagggctt acctgttctt atacataaaa gcaacagaat
    34321 ggtaacattc tatcgcgggt aagccaattg atccccgtca tttatctggc tatatcctga
    34381 gcggcctttg ctttgtctgt ttcttacttt tgccctgacg ttttattgga tttttatcga
    34441 cgatactctc cgtttaagcg gcaggtttcc gctgtacgta aaagaaccgg ccagtaaata
    34501 tgttttattg cgttaccgtt cattcacaat actggagcaa tccagtatgt tcattctctg
    34561 gtatagtgcc agcagtactt ttggcaagga ttcagacatc gtgatgggca taagagcgca
    34621 acaaaaagaa aaaacccgcc gttcgctggt ggaagccgca tttagccaat taagtgctga
    34681 acgcagcttc gccagcctga gtttgcgtga agtggcgcgt gaagcgggca ttgctcccac
    34741 ctctttttat cggcatttcc gcgacgtaga cgaactgggt ctgaccatgg ttgatgagag
    34801 cggtttaatg ctacgccaac tcatgcgcca ggcgcgtcag cgtatcgcca aaggcgggag
    34861 tgtgatccgc acctcggtct ccacatttat ggagttcatc ggtaataatc ctaacgcctt
    34921 ccggttatta ttgcgggaac gctccggcac ctccgctgcg tttcgtgccg ccgttgcgcg
    34981 tgaaattcag cacttcattg cggaacttgc ggactatctg gaactcgaaa accatatgcc
    35041 gcgtgcgttt actgaagcgc aagccgaagc aatggtgaca attgtcttca gtgcgggtgc
    35101 cgaggcgttg gacgtcggcg tcgaacaacg tcggcaatta gaagagcgac tggtactgca
    35161 actgcgaatg atttcgaaag gggcttatta ctggtatcgc cgtgaacaag agaaaaccgc
    35221 aattattccg ggaaatgtga aggacgagta atgaaacaag caaatcaaga tagaggtacg
    35281 ctgctgctgg cgttagttgc tggcttatcg attaatggta ctttcgcagc gctgtttagc
    35341 tccattgtgc cattttctgt attcccgatt atttccctgg tgctgacggt ttactgcctg
    35401 catcaacgtt atcttaatcg cactatgccg gtaggcttgc cgggtctggc agctgcctgt
    35461 tttattctcg gcgtactgct gtacagcacg gtagttcgtg cggaatatcc ggatatcggc
    35521 tctaacttct tcccggcagt actctccgtc attatggtgt tctggattgg cgcgaagatg
    35581 cgtaaccgta agcaggaagt tgctgagtaa tcggtattat gccggatgct ggcgcatccg
    35641 gcatgggttt tacttcgcgg tcagtaatac gccgcactcc atatggtgcg tgtaggggaa
    35701 ctgatcaaac agagccagac gttcgacctt gtgcgtctgg cttaatgttt ccagattctt
    35761 gcataacgtt tccgggttac aggagatgta caaaatacac ggatacgcct gcaccatttt
    35821 ctcggtttca ctgtccagac cgctgcgcgg tgggtcgaca aaaatggttt cgcactgata
    35881 actctttaag tcgatccctt gcaggcggtt aaactcgcgc acaccattca tcgcctgagt
    35941 aaattcttct gccgccatac gaataatttg tacgttatca atatggttag ctgcgatgtt
    36001 gtattgcgca gcagcaaccg acggcttagc gatttcggtg gctaataccc gatcaaaatt
    36061 gcgcgccagc gctaatgaaa agttaccgtt gccgcagtac agctccagta aatcgccttt
    36121 tgagcctttg gttacgtcca gcgcccattc cagcatctga atattcatcg ccgcgttcgg
    36181 ctgggtaaag ctgttttcta cctgacggta gatcatctct ttccctgcga ccggcagacg
    36241 ctcgtcgatg taatcttgat ccagctcgat tttggttttc gttgcccgac caatcagatg
    36301 cacattcaga ttctgcgcgc gcagtgcatc gcgcagggcc tccgcttcct gacgccactc
    36361 atcatccagc ttcttatggt atagcaggga aaccaccgcc tgattactca gcgtggtgag
    36421 gtaatcaatc tggaacaact tgtggcgcag aacgggatta ttacgcacac ccgcaatcat
    36481 cgccgtcatc aactggttga taagttcact ggcggcgggg aagctatcca cgcggatacg
    36541 gcttttggtt tgttgatcga aaatgatgtg atacaggtca tcgccatcgt gccagatgcg
    36601 gaactccgca cgcatccggt aatgactgac cggcgagcga aacacttccg gaaccaggtc
    36661 agaaaacggt gccatcatac tttgcaaacg taccactttt tcggctaact gcgcttcata
    36721 ctgttctgtt ggaaggtgtt cgggggtcat gatgtatcct gaaaaattaa agtacgcggc
    36781 gattgtaggg attgctcatc agatgtccag atcttgatga attcctattt gtgagctacg
    36841 tctggacagt aacttgttac aacctgtagc atccacttgt cggtcctgtg agttaatagg
    36901 gaatccagtg cgaatctgga gctgacgcgc agcggtaagg aaaggtgcga tgattgcgtt
    36961 atgcggacac tgccatccgg tgggaagtca tcatctctta gtatcttaga tacccctcca
    37021 agcccgaaga cctgccggcc aacgtcgcat ctggttctca tcatcgcgta atattgatga
    37081 aacctgcggc atccttcttc tattgtggat gctttacaat gattaaaaaa gcttcgctgc
    37141 tgacggcgtg ttctgtcaca gccttttccg cttgggcaca ggataccagc ccggatactc
    37201 tcgtcgttac tgctaaccgt tttgaacagc cgcgcagcac tgtgcttgca ccaaccaccg
    37261 ttgtgacccg tcaggatatc gaccgctggc agtcgacctc ggtcaatgat gtgctgcgcc
    37321 gtcttccggg cgtcgatatc acccaaaacg gcggttcagg tcagctctca tctattttta
    37381 ttcgcggtac aaatgccagt catgtgttgg tgttaattga tggcgtacgc ctgaatctgg
    37441 cgggggtgag tggttctgcc gaccttagcc agttccctat tgcgcttgtc cagcgtgttg
    37501 aatatatccg tgggccgcgc tccgctgttt atggttccga tgcaataggc ggggtggtga
    37561 atatcatcac gacgcgcgat gaacccggaa cggaaatttc agcagggtgg ggaagcaata
    37621 gttatcagaa ctatgatgtc tctacgcagc aacaactggg ggataagaca cgggtaacgc
    37681 tgttgggcga ttatgcccat actcatggtt atgatgttgt tgcctatggt aatacaggaa
    37741 cgcaagcgca gacagataac gatggttttt taagtaaaac gctttatggc gcgctggagc
    37801 ataactttac tgatgcctgg agcggctttg tgcgcggcta tggctatgat aaccgtacca
    37861 attatgacgc gtattattct cccggttcac cgttgctcga tacccgtaaa ctctatagcc
    37921 aaagttggga cgccgggctg cgctataacg gcgaactgat taaatcacaa ctcattacca
    37981 gctatagcca tagcaaagat tacaactacg atccccatta tggtcgttat gattcgtcgg
    38041 cgacgctcga tgagatgaag caatacaccg tccagtgggc aaacaatgtc atcgttggtc
    38101 acggtagtat tggtgcgggt gtcgactggc agaaacagac tacgacgccg ggtacaggtt
    38161 atgttgagga tggatatgat caacgtaata ccggcatcta tctgaccggg ctgcaacaag
    38221 tcggcgattt tacctttgaa ggcgcagcac gcagtgacga taactcacag tttggtcgtc
    38281 atggaacctg gcaaaccagc gccggttggg aattcatcga aggttatcgc ttcattgctt
    38341 cctacgggac atcttataag gcaccaaatc tggggcaact gtatggcttc tacggaaatc
    38401 cgaatctgga cccggagaaa agcaaacagt gggaaggcgc gtttgaaggc ttaaccgctg
    38461 gggtgaactg gcgtatttcc ggatatcgta acgatgtcag tgacttgatc gattatgatg
    38521 atcacaccct gaaatattac aacgaaggga aagcgcggat taagggcgtc gaggcgaccg
    38581 ccaattttga taccggacca ctgacgcata ctgtgagtta tgattatgtc gatgcgcgca
    38641 atgcgattac cgacacgccg ttgttacgcc gtgctaaaca gcaggtgaaa taccagctcg
    38701 actggcagtt gtatgacttc gactggggta ttacttatca gtatttaggc actcgctatg
    38761 ataaggatta ctcatcttat ccttatcaaa ccgttaaaat gggcggtgtg agcttgtggg
    38821 atcttgcggt tgcgtatccg gtcacctctc acctgacagt tcgtggtaaa atagccaacc
    38881 tgttcgacaa agattatgag acagtctatg gctaccaaac tgcaggacgg gaatacacct
    38941 tgtctggcag ctacaccttc tgaaccacgt cccaccgtgc tggtgtttga ctccggcgtc
    39001 ggtgggttgt cggtctatga cgagatccgg catctcttac cggatctcca ttacatttat
    39061 gctttcgata acgtcgcttt cccgtatggc gaaaaaagcg aagcgtttat tgttgagcga
    39121 gtggtggcaa ttgtcaccgc ggtgcaagaa cgttatcccc ttgcgctggc tgtggtcgct
    39181 tgcaacactg ccagtaccgt ttcacttcct gcattacgcg aaaagttcga cttcccggtt
    39241 gttggtgtcg tgccggcgat taaacctgct gcacgtctga cggcaaatgg cattgtcgga
    39301 ttactggcaa cccgcggaac agttaaacgt tcttatactc atgagctgat cgcgcgtttc
    39361 gctaatgaat gccagataga aatgctgggc tcggcagaga tggttgagtt ggctgaagcg
    39421 aagctacatg gcgaagatgt ttctctggat gcactaaaac gtatcctacg cccgtggtta
    39481 agaatgaaag agccgccaga taccgttgta ttgggttgca cccatttccc tctactacaa
    39541 gaagaactgt tacaagtgct gccagaggga acccggctgg tggattctgg cgcagcgatt
    39601 gctcgccgaa cggcctggtt gttagaacat gaagccccgg atgcaaaatc tgccgatgcg
    39661 aatattgcct tttgtatggc aatgacgcca gaagctgaac aattattgcc cgttttacag
    39721 cgttacggct tcgaaacgct cgaaaaactg gcagttttag gctgatttgg ttgaatgttg
    39781 cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc ggaataactc cctataatgc
    39841 gccaccactg acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga
    39901 atctcaacag agaaaagcaa aaaaaatgct tgactctgta gcgggaaagc gtattatgca
    39961 cacc
//
LOCUS       DAZPBM010000020        38790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPBM010000020 DAZPBM010000000
VERSION     DAZPBM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.020"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..265
                     /locus_tag="ACHUZQ_000938"
     CDS             <1..265
                     /locus_tag="ACHUZQ_000938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024706.1"
                     /translation="APVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAK
                     SLAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
     gene            219..1883
                     /gene="asnB"
                     /locus_tag="ACHUZQ_000939"
     CDS             219..1883
                     /gene="asnB"
                     /locus_tag="ACHUZQ_000939"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1024707.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2263..2339
                     /locus_tag="ACHUZQ_000940"
     tRNA            2263..2339
                     /locus_tag="ACHUZQ_000940"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2297..2299,aa:Met,seq:cat)
     gene            2348..2432
                     /locus_tag="ACHUZQ_000941"
     tRNA            2348..2432
                     /locus_tag="ACHUZQ_000941"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2382..2384,aa:Leu,seq:tag)
     gene            2456..2530
                     /locus_tag="ACHUZQ_000942"
     tRNA            2456..2530
                     /locus_tag="ACHUZQ_000942"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2488..2490,aa:Gln,seq:ttg)
     gene            2565..2639
                     /locus_tag="ACHUZQ_000943"
     tRNA            2565..2639
                     /locus_tag="ACHUZQ_000943"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2597..2599,aa:Gln,seq:ttg)
     gene            2655..2731
                     /locus_tag="ACHUZQ_000944"
     tRNA            2655..2731
                     /locus_tag="ACHUZQ_000944"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2689..2691,aa:Met,seq:cat)
     gene            2780..2854
                     /locus_tag="ACHUZQ_000945"
     tRNA            2780..2854
                     /locus_tag="ACHUZQ_000945"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2812..2814,aa:Gln,seq:ctg)
     gene            2891..2965
                     /locus_tag="ACHUZQ_000946"
     tRNA            2891..2965
                     /locus_tag="ACHUZQ_000946"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2923..2925,aa:Gln,seq:ctg)
     gene            complement(3119..4294)
                     /gene="ubiF"
                     /locus_tag="ACHUZQ_000947"
     CDS             complement(3119..4294)
                     /gene="ubiF"
                     /locus_tag="ACHUZQ_000947"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1024708.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4440..5864
                     /gene="miaB"
                     /locus_tag="ACHUZQ_000948"
     CDS             4440..5864
                     /gene="miaB"
                     /locus_tag="ACHUZQ_000948"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1024709.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6017..7057
                     /locus_tag="ACHUZQ_000949"
     CDS             6017..7057
                     /locus_tag="ACHUZQ_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1024710.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7054..7521
                     /gene="ybeY"
                     /locus_tag="ACHUZQ_000950"
     CDS             7054..7521
                     /gene="ybeY"
                     /locus_tag="ACHUZQ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1024711.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7611..8489
                     /gene="corC"
                     /locus_tag="ACHUZQ_000951"
     CDS             7611..8489
                     /gene="corC"
                     /locus_tag="ACHUZQ_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1024712.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8514..10052
                     /gene="lnt"
                     /locus_tag="ACHUZQ_000952"
     CDS             8514..10052
                     /gene="lnt"
                     /locus_tag="ACHUZQ_000952"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1024713.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10130..12007)
                     /locus_tag="ACHUZQ_000953"
     CDS             complement(10130..12007)
                     /locus_tag="ACHUZQ_000953"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR1024714.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12462..13370
                     /gene="gltI"
                     /locus_tag="ACHUZQ_000954"
     CDS             12462..13370
                     /gene="gltI"
                     /locus_tag="ACHUZQ_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1024715.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13540..14280
                     /gene="gltJ"
                     /locus_tag="ACHUZQ_000955"
     CDS             13540..14280
                     /gene="gltJ"
                     /locus_tag="ACHUZQ_000955"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1024716.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14280..14954
                     /gene="gltK"
                     /locus_tag="ACHUZQ_000956"
     CDS             14280..14954
                     /gene="gltK"
                     /locus_tag="ACHUZQ_000956"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1024717.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14954..15679
                     /gene="gltL"
                     /locus_tag="ACHUZQ_000957"
     CDS             14954..15679
                     /gene="gltL"
                     /locus_tag="ACHUZQ_000957"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1024718.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15797..16732
                     /gene="rihA"
                     /locus_tag="ACHUZQ_000958"
     CDS             15797..16732
                     /gene="rihA"
                     /locus_tag="ACHUZQ_000958"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1024719.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16840..18510
                     /gene="hscC"
                     /locus_tag="ACHUZQ_000959"
     CDS             16840..18510
                     /gene="hscC"
                     /locus_tag="ACHUZQ_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR1024720.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18570..20021)
                     /gene="djlC"
                     /locus_tag="ACHUZQ_000960"
     CDS             complement(18570..20021)
                     /gene="djlC"
                     /locus_tag="ACHUZQ_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1024721.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(20018..20725)
                     /locus_tag="ACHUZQ_000961"
     CDS             complement(20018..20725)
                     /locus_tag="ACHUZQ_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1024722.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20827..21381
                     /locus_tag="ACHUZQ_000962"
     CDS             20827..21381
                     /locus_tag="ACHUZQ_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1024723.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21391..22818)
                     /locus_tag="ACHUZQ_000963"
     CDS             complement(21391..22818)
                     /locus_tag="ACHUZQ_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1024724.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22815..23522)
                     /locus_tag="ACHUZQ_000964"
     CDS             complement(22815..23522)
                     /locus_tag="ACHUZQ_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1024725.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23686..24663
                     /gene="ybeQ"
                     /locus_tag="ACHUZQ_000965"
     CDS             23686..24663
                     /gene="ybeQ"
                     /locus_tag="ACHUZQ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1024726.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24733..25215)
                     /locus_tag="ACHUZQ_000966"
     CDS             complement(24733..25215)
                     /locus_tag="ACHUZQ_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1024727.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25450..28032
                     /gene="leuS"
                     /locus_tag="ACHUZQ_000967"
     CDS             25450..28032
                     /gene="leuS"
                     /locus_tag="ACHUZQ_000967"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1024728.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28047..28628
                     /gene="lptE"
                     /locus_tag="ACHUZQ_000968"
     CDS             28047..28628
                     /gene="lptE"
                     /locus_tag="ACHUZQ_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1024729.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28628..29659
                     /gene="holA"
                     /locus_tag="ACHUZQ_000969"
     CDS             28628..29659
                     /gene="holA"
                     /locus_tag="ACHUZQ_000969"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1024730.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29661..30302
                     /gene="nadD"
                     /locus_tag="ACHUZQ_000970"
     CDS             29661..30302
                     /gene="nadD"
                     /locus_tag="ACHUZQ_000970"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1024731.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30326..30937
                     /locus_tag="ACHUZQ_000971"
                     /pseudo
     CDS             30326..30937
                     /locus_tag="ACHUZQ_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31197..31514
                     /gene="rsfS"
                     /locus_tag="ACHUZQ_000972"
     CDS             31197..31514
                     /gene="rsfS"
                     /locus_tag="ACHUZQ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1024732.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31518..31985
                     /gene="rlmH"
                     /locus_tag="ACHUZQ_000973"
     CDS             31518..31985
                     /gene="rlmH"
                     /locus_tag="ACHUZQ_000973"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1024733.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            32016..33917
                     /gene="mrdA"
                     /locus_tag="ACHUZQ_000974"
     CDS             32016..33917
                     /gene="mrdA"
                     /locus_tag="ACHUZQ_000974"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1024734.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33920..35032
                     /gene="mrdB"
                     /locus_tag="ACHUZQ_000975"
                     /gene_synonym="rodA"
     CDS             33920..35032
                     /gene="mrdB"
                     /locus_tag="ACHUZQ_000975"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1024735.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35043..36131
                     /gene="rlpA"
                     /locus_tag="ACHUZQ_000976"
     CDS             35043..36131
                     /gene="rlpA"
                     /locus_tag="ACHUZQ_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1024736.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36270..37481
                     /gene="dacA"
                     /locus_tag="ACHUZQ_000977"
     CDS             36270..37481
                     /gene="dacA"
                     /locus_tag="ACHUZQ_000977"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1024737.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37591..37854
                     /gene="ybeD"
                     /locus_tag="ACHUZQ_000978"
     CDS             37591..37854
                     /gene="ybeD"
                     /locus_tag="ACHUZQ_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1024738.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37955..38596
                     /gene="lipB"
                     /locus_tag="ACHUZQ_000979"
     CDS             37955..38596
                     /gene="lipB"
                     /locus_tag="ACHUZQ_000979"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1024739.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         9696 a         9904 c         9791 g         9399 t
ORIGIN      
        1 ggcccccgta aaatataacc attcaataaa acccgctaaa aattatcgat tcatcaataa
       61 atacatcctc acaccagccc ctttttcacc aatcgacaat caccattacg ttttttattt
      121 tttcaccgcc aaatcgcttg cgcacagtgc tgctttgcgg cattttttta aacaagcaaa
      181 cacaacaagc aacaaatacc aggttaacgg agaaggttat gtgttcaatt tttggcgtat
      241 tcgatatcaa aacagacgca gttgagcagc gtaagaaagc actcgagctg tcacgcctga
      301 tgcgtcatcg tggcccggac tggtccggta tttatgccag cgataacgcc attctcgctc
      361 acgaacgtct gtcaattgtt gacgttaacg cgggggcgca acctctctac aaccaacaaa
      421 aaacccacgt actggcggta aacggtgaaa tctacaacca ccaggcattg cgcgctgaat
      481 atggcgatcg ttatcagttc cagacagggt ctgactgtga agtgatcctc gcgctgtatc
      541 aggaaaaagg gccggaattt ctcgacgact tgcagggcat gtttgccttt gccctgtacg
      601 acagcgaaaa agatgcctac ctgattggtc gcgaccatct ggggatcatc ccactgtata
      661 tggggtatga cgaacacggt cagctgtatg tggcctcaga aatgaaatcg ctggtgccag
      721 tttgccgcac gattaaagag ttcccggcgg ggagctattt gtggagccag gacggcgaaa
      781 tccgttctta ttatcatcgc gactggttcg actttgatgc agtgaaagat aacgtgaccg
      841 acaaaaacga gctgcgtcag gctctggaag attccgttaa aagccatctg atgtctgatg
      901 tgccttacgg tgtgctgctt tctggtggtc tggattcctc aattatttcc gctatcacca
      961 agaaatacgc agcccgtcgc gtggaagatc aggaacgctc tgaagcctgg tggccccagt
     1021 tacactcctt tgctgtaggt ctgccgggtt caccggatct taaagcagcc caggaagtgg
     1081 caaaccatct gggcacggtg catcacgaaa ttcacttcac tgtacaggaa ggtctggatg
     1141 ccatccgcga cgtgatttac cacatcgaaa cttatgatgt gaccactatt cgcgcttcaa
     1201 caccgatgta tttaatgtcg cgtaagatca aggcgatggg cattaaaatg gtgctgtccg
     1261 gtgaaggttc tgatgaagtg tttggcggtt atctttactt ccataaagcg cccaacgcta
     1321 aagaactgca tgaagagacg gtgcgtaaac tgctggccct gcatatgtat gactgcgcgc
     1381 gcgccaacaa agcgatgtca gcctggggcg tggaagcacg cgttccgttc ctcgacaaaa
     1441 aattccttga tgtggcgatg cgcattaacc cgcaggataa aatgtgcggt aacggcaaaa
     1501 tggaaaaaca catcctgcgt gaatgttttg agtcatacct gcccgcaagc gtggcctggc
     1561 ggcagaaaga gcagttctcc gatggcgtcg gttacagttg gatcgacacc ctgaaagaag
     1621 tggcggcgca gcaggtttct gatcagcaac tggaaactgc ccgcttccgc ttcccgtaca
     1681 acacgccaac ctcaaaagaa gcgtatctgt accgggagat ctttgaagaa ctgttcccgc
     1741 ttccgagcgc cgctgagtgc gtgccgggcg gcccgtccgt cgcgtgttct tccgctaaag
     1801 cgattgagtg ggatgaggcg ttcaagaaaa tggacgatcc gtctggtcgt gcggttggcg
     1861 ttcaccagtc ggcgtataag taagaaatgc gaaacagccc cggtgaaatc cggggctgtt
     1921 tcagttattg aggtgcttgt tgattctgat atgcagtaag gaaatgttcc gggtttgcca
     1981 catcactgtt tgacaactca tcactgtagg ccagtatttc atagattaat ttcccaccac
     2041 cattgttgaa ttccgaattt catcctgtat gacaaaagaa tggctgtagg gtggggaaaa
     2101 aacacaaagt aaacaataat tgacgaatat agcgccacgc tgttcgcaac ctaaccaaac
     2161 agtcactttc gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat
     2221 aatgcgcccc gcaacgccga taaggtatcg cgaaaaaaag atggctacgt agctcagttg
     2281 gttagagcac atcactcata atgatggggt cacaggttcg aatcccgtcg tagccaccat
     2341 cttttttgcg ggagtggcga aattggtaga cgcaccagat ttaggttctg gcgccgcaag
     2401 gtgtgcgagt tcaagtctcg cctcccgcac cattcaccag aaagcgttgt acggatgggg
     2461 tatcgccaag cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt
     2521 accccagcca tcttcttcga gtaagcggtt caccgaccgg ttattggggt atcgccaagc
     2581 ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta ccccagccat
     2641 cgaagaaaca atctggctac gtagctcagt tggttagagc acatcactca taatgatggg
     2701 gtcacaggtt cgaatcccgt cgtagccacc aaattctgaa tgtgtcgaat acgttcggca
     2761 aattcaaaaa ccaatttgtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg
     2821 gcattccgag gttcgaatcc tcgtacccca gccaatttat tcaagacgct tacatcgtaa
     2881 gtgcaccagt tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga
     2941 ggttcgaatc ctcgtacccc agccacatta aaaaagctcg cttcggcgag ttttttgctt
     3001 ttctgcgttc attcaatgtc gaatgcgatg ttgacacgtc ttatccttca atgtcggatg
     3061 cgacgctgcc gcgtcttatc cgacctacgg ttggcacgca tccggcaatg ttgtaaggct
     3121 acaaccctaa cgcatatttc agcgcctgac gtttcaacac gccagcacgc tctgccgcca
     3181 ttaacccgag attacgcaca aaacgcagcg gcggcagatt attactgaat ccggcataaa
     3241 acagatccat accactttgc ataatgaagt tatccgccat gcgccgcatc tggtaacgct
     3301 tgaggacagg ataactggcc cacgcttcac cgtagctgcg ggcattcacc agaacatcaa
     3361 tcagggcatc gacatcacga taaccaagat tcaccccctg ccccgccagc ggatggatgg
     3421 tatgcgcggc atcgcccacc agcgcaagcc ccggctgcac atactgcaac gcatgtcggc
     3481 gcgtcagcgg aaacgcacca gcggcaagcg gtgtaacgta acccagacgc gacgggaaat
     3541 gcttcgcgat ttccgcctgg aattgcgcca tattcatatt ctgcaactgg cgaatacgcg
     3601 ccggagagtc ataccacacc agcgatgccc agttatcaaa caacggcaga aacgcacgcg
     3661 gtccgtccgg ggtaaattgc tgccaggtgc tgtcgccggg atcgttctca cactgtacgc
     3721 taatcaacat gcacgactgc gcatactgcc aggcatgaac gccaattccc gccatctgcc
     3781 gcacctgaga atttgcgccg tcggcaccaa tcaccagttt cgcgcggatc gtttcaccgc
     3841 ccttcagttc cagctcctga agatcattat ctcgatgtaa tgcaatcagc gagcctggca
     3901 cacgtaacgt tactttcgga tgcgcttcca gcgcctgcca cagcgcctgt tgcaggacgg
     3961 tgttttccac catatagcca agcaacggta gcttaagttc agcggcgtca aacaccacat
     4021 gcgccgtttc ccactcccac gtttccagtc tgcggtaagg atggcaacgc atagcctgta
     4081 ctgcatccca gacccctaac cctttaagca atgataccga tgcggcgctg atcgccgaga
     4141 tccgcacgtc cggttggcta tcagcgacaa acggtgcagg ttctgcgtgc tcgatcaccg
     4201 ttaccgaaaa tccgtgctgt gccagcccca gcgccagcgc gccgccgacc attcctccgc
     4261 cgacaatggc aatttccgtt ggttgatttg tcatggtcaa tcatcctgtt agcaatatgc
     4321 tttaagttta caggattttt acccccaagg gctgataacg ctcatactgg tcacaacggc
     4381 agcaaagcat tacactatac gccctgcatt cctggctact atttcgcaag agcaagtcga
     4441 tgaccaaaaa actccatatt aaaacctggg gctgtcagat gaacgagtac gattcatcga
     4501 agatggccga tctgctggat gccacccacg gctatcaact gaccgacgtg gcggaagaag
     4561 cggatgtgct gctgctgaac acctgctcaa tccgcgagaa ggctcaggaa aaagtcttcc
     4621 atcagttggg tcgctggaaa ctgttaaaag agaagaatcc agacctgatt atcggcgtcg
     4681 gtggctgcgt ggcatcgcaa gaaggcgagc acattcgcca gcgcgcccac tatgtcgata
     4741 ttatttttgg gccgcaaacg ctgcaccgtc tgccggagat gatcaactcc gtgcgcggcg
     4801 accgcagccc ggttgtagat atcagcttcc cggaaatcga gaagtttgac cgtctaccgg
     4861 aaccgcgcgc cgaagggccg accgcgtttg tctccatcat ggaaggctgc aataaatatt
     4921 gcacctactg cgtggtgcct tacacccgtg gtgaagaggt aagccgtccg tctgacgata
     4981 ttctgtttga gattgcccag cttgcggctc agggcgtgcg tgaagtcaac ctgctcggtc
     5041 agaacgtgaa cgcctggcgt ggtgaaaact acgacggctc caccggatcg tttgccgatc
     5101 tgctgcgtct ggttgctgcg atcgacggga tcgatcgtat tcgctttacc accagccatc
     5161 cgatcgaatt caccgacgat atcatcgaag tgtatcgcga cacgccggag ctggtgagct
     5221 tcctgcatct gccggtacaa agcggttccg atcgcattct gaacctgatg ggccgtactc
     5281 acacggcgct ggagtacaaa gcgatcatcc gtaaactgcg tgcggcgcgt ccggatattc
     5341 agatcagttc tgacttcatt gttggcttcc ctggcgaaac caccgaagac ttcgagaaaa
     5401 cgatgaagct gattgccgac gtcaatttcg acatgagcta cagctttatc ttctctgcac
     5461 gtccgggtac accagccgcc gatatggttg atgatgttcc ggaagaagag aagaagcaac
     5521 gtctgtatat tctgcaagaa cgtatcaatc agcaagcgat ggcatggagc cgccggatgc
     5581 tcggcaccac ccagcgtatt ctggtagaag ggacttcgcg taagagcatc atggagcttt
     5641 ccgggcgtac cgaaaataac cgcgtggtca acttcgaggg cacaccggat atgatcggta
     5701 aattcgtcga tgtagaaatt accgacgtct acccgaactc tctgcgcggt aaagtggtgc
     5761 gtactgaaga tgaaatgggt ctgcgcgtgg cagaaacacc ggaatcggtg attgcccgta
     5821 cccgcaaaga aaacgacctt ggcgtgggtt attatcagcc gtaattctca ggcccgccgt
     5881 tccggtgggc cttgtttttt ctctctttat ccccatcttt atcgcaatgc ttgcgccttt
     5941 ttcctgtggc gtgaataatt tccttatagg ccggtgaatg ttcagcttcc ggcggcatac
     6001 agataagagg aacggtttga acatagacac tcgcgaaatc accctggagc cagcagacaa
     6061 cgcgcgtctg ttgagcctgt gcggcccgtt tgatgacaac atcaagcagc tcgaacgccg
     6121 tctcggcatc gagatcaatc gccgcgacaa ccactttaaa ctgaccggcc gtccgatttg
     6181 cgtcaccgct gcggcagaca ttctgcgtag cctgtatgtc gatacagccc cgatgcgcgg
     6241 tcagattcag gatatcgaac cggaacagat ccaccttgcg attaaagaag cgcgagtact
     6301 ggagcaaagt gcggagagcg tgccggagta cggcaaagcg gtcaatatca aaaccaaacg
     6361 cggcgtaatt aagccgcgca cgccaaacca ggcgcagtac atcgccaata ttctcgacca
     6421 tgacattacc ttcggcgttg gcccggcggg tacgggtaaa acctacctgg cagtggctgc
     6481 ggcagttgat gccctggagc gtcaggagat tcgccgtatt ctgctgactc gtccggcggt
     6541 cgaagccggt gagaaactgg gcttcctgcc tggcgattta agccagaaag tagacccgta
     6601 tctgcgccca ctgtacgacg cgctgtttga aatgctgggc tttgagaaag ttgagaaact
     6661 gattgagcgc aacgttattg aagtcgcgcc gctggcctat atgcgtggtc gtacgctgaa
     6721 cgacgcgttt atcattctcg atgagagcca gaacaccacc atcgaacaga tgaagatgtt
     6781 cctgacccgt atcggtttta actcaaaagc ggttatcacc ggcgacgtca cacagatcga
     6841 cctgccgcgt aatactaaat caggcttacg tcacgccatc gaagtgctgg ccgatgtcga
     6901 agagatcagc tttaacttct tccacagcga agacgtggtt cgtcaccctg tggtggcgcg
     6961 tatcgttaac gcctatgaag cctgggaaga agccgaacaa aaacgaaaag cggcgctggc
     7021 ggcagaacgc aagcgcgaag aacaggaaca aaaatgagtc aggtgatcct cgatttacaa
     7081 ctggcatgtg aagataattc cgggttaccg gaagagagcc agtttcaaac atggctgaat
     7141 gcggtgatcc cgcagtttca ggaagaatcg gaagtgacta ttcgcgtggt cgataccgcc
     7201 gaaagccaca gtctgaatct gacctatcgc ggtaaggata agccgaccaa cgtgctctcc
     7261 ttcccgtttg aagtgccgcc tggcatggaa atgtcgctac tgggcgatct ggttatctgc
     7321 cgtcaggtgg ttgagaagga agctcaggag caaggcaaac cactggaggc gcactgggcg
     7381 catatggtgg tgcacggcag tctgcatttg ttaggttacg atcacatcga agatgacgaa
     7441 gcagaagaaa tggaagccct cgaaacagag attatgcttg ctctgggcta tgaggatccg
     7501 tacattgccg agaaagaata atttgccagg catttgactg gcagacgtcc ccgccgttgg
     7561 tgattaacga cggcgttttg attaactaac atgagaaccc atacgacgcc atgagcgacg
     7621 acaattcaca cagtagtgac acgataagca acaagaaggg atttttctcc ctgttactca
     7681 gccaactttt ccacggtgaa ccgaaaaacc gtgacgaact gctggcgctg atccgtgatt
     7741 ccgggcagaa cgaccttatc gacgaagata cgcgcgatat gctcgaaggg gtgatggaca
     7801 tcgcagacca acgcgttcgc gacatcatga tcccccgctc ccagatgatt accctgaaac
     7861 gcaaccagac gctggacgaa tgccttgatg tcatcatcga gtccgcccac tcacgtttcc
     7921 cggtgattag cgaagacaaa gatcacattg aagggattct gatggcgaaa gacttgctgc
     7981 cgtttatgcg cagcgatgct gaagccttca gcatggacaa agtgttacgt caggcggttg
     8041 tcgttcctga aagtaagcgc gtagaccgga tgctgaaaga gtttcgctct cagcgttacc
     8101 acatggcgat cgtcattgac gaattcggtg gggtttccgg tctggtgacc attgaagaca
     8161 tcctggaact gattgttggt gagattgaag acgagtatga cgaagaagat gatatcgact
     8221 tccgtcagct gagtcgtcat acctggaccg tgcgtgcact ggcttccatt gaagacttca
     8281 acgaagcgtt cggcacccac tttagcgatg aagaggttga cactatcggt ggtctggtga
     8341 tgcaggcatt tgggcatctt ccggcgcgtg gcgaaactat cgacatcgac ggttaccagt
     8401 tcaaagtggc gatggccgac agtcggcgta ttattcaggt tcatgtcaaa atcccggatg
     8461 actcacccca gccgaagctg gatgaataaa accgaaactg gatagataac tacatggctt
     8521 ttgcctcatt aattgaacgc cagcgcattc gcctgctgct ggcgttatta ttcggtgcct
     8581 gcggaacgct ggccttctct ccttacgacg tctggcctgc ggcgattatt tcgttgatgg
     8641 ggcttcaggc gttgaccttt aaccgccgtc cactccagtc tgccgctatt ggcttttgct
     8701 ggggatttgg cctctttggc agcggtatta actgggtcta tgtcagcatc gcgacctttg
     8761 gcggaatgcc tggcccggtt aacatcttcc tggtggtgct gctggcggcg tatttgtcgc
     8821 tgtataccgg actgtttgct ggcgtgctgt cgcgtctgtg gccgaaaacc acctggctgc
     8881 gcgtggcgat tgccgcccct gctctctggc aagtgaccga gtttctgcgc ggttgggtac
     8941 tgacaggctt cccgtggtta cagttcggct atagccagat tgatggcccg ttaaaagggc
     9001 tggcaccgat aatgggcgtg gaagccatta acttcctgct gatgatggtt agtggcctgc
     9061 tggcactggc gttggtcaaa cgcaactggc gtccgctggt ggtggccgtc gtgctgtttg
     9121 ccctaccctt cccgctgcgt tacatccaat ggtttacccc gcaaccggag aaaaccattc
     9181 aggtttcgat ggttcagggc gatattccgc aatcactgaa atgggatgga gatcagcttc
     9241 ttaatacgct gaagatttac tacaacgcaa cggctccgct gatgggcaaa tcatcgttga
     9301 ttatctggcc ggagtcggcg ataaccgatc tggaaattaa tcagcaaccg ttcctcaaag
     9361 cactggacgg tgagttgcgc gataaaggta gctcgctggt aaccgggatt gtcgacgcgc
     9421 gtctcaataa gcagaaccgc tacgatacct acaacaccat catcacactg ggtaaaggtg
     9481 caccatacag ctacgaatca gccgatcgct ataacaaaaa ccatctagtg ccgtttggcg
     9541 agtttgtccc gctggagtcg attctgcgtc cgttagcacc gttctttgat ctgccgatgt
     9601 cgtcgttcag ccgtgggcca tatatccagc cgccgctgtc ggtaaatggt attgagctta
     9661 ctgcggctat ttgctacgag atcattctcg gcgagcaagt gcgcgataac ttccgcccgg
     9721 ataccgacta tctgctgact atctccaacg atgcgtggtt tggtaagtct attggtccat
     9781 ggcagcactt ccagatggcg cgaatgcgtg cgctggagct ggcgcgccca ctgttgcgca
     9841 gcaccaacaa cggcattacg gcggtgattg gcccgcaggg ggaggttcag gcgatgatcc
     9901 cgcagttcac ccgcgaggtg ttgaccacta atgtgacacc gaccaccgga ctcacaccgt
     9961 atgcacgtac cggcaactgg ccactgtggg tgctgacggc gctgtttggt tttgccgctg
    10021 tgttgatgag tctgcgagca cgtaaacgtt gatgtgcctg atgtatcctg catgagcaaa
    10081 acgtactttc tcattgagct ttggggcatg gttatcatgc ccctttttct tacggttcag
    10141 ggatacaagc cacattgcca tattggggat gacgaataat tgcctgcgga tcaagcgagc
    10201 gcgtcctgtc catttggatc aacccgttct tcgccatgac aaatattttt ctaccatccg
    10261 ctgaaaaacg aaggtgatta acgtagctgg caggataaac gccaataatc tgcccggcat
    10321 taatattgag caggatgaca taaccttgca ggccatatga caccaacgct aaatattgac
    10381 cgtccgggct aataccctca acggaataaa cgctcctgct acaccattgc gtcataatgc
    10441 tggcttgttg atcccctacg cttatacgtg aaatcccctg actatcggca aaaataacct
    10501 cgccgttttc aggtcgaaaa cgcatcgccg atatcgttcc acgcgcatca cctgcgggaa
    10561 agtcttcttt ctgatgcgtg atggtattga tccgcacgat gttacctctg cctgctcccg
    10621 gaaccagtaa tgttctttca tccggggaaa gtgccataac ccaggcgcct ggtttaccgc
    10681 tcgtccccgt cccgtcgtat ttctccagcg ggatatccgc cacatgctga taagtcacca
    10741 gatcaatcgc tgacacacta ttactggtgc gattcatcac ccaggctttt gtgccatcac
    10801 gcgagaggat aaggctgtca ggatgtgcac cggtggtaat cgactgaata atctcccctg
    10861 tcgccacgtt aatgaccgat aacgcattcg cttcaaatga agtcgcataa atcaactttt
    10921 catccgggct taatgccagc gatctgacgc cctgatcttt acagccacca caatgtttga
    10981 ctgacttttc tttcgctatg ggcgctgtaa aggtatgcag gattttcttt tgtccgaggt
    11041 cgaaaacaac aatgctgttc gtgttttcaa ttgcggcata caacatatta ccatcccgac
    11101 tgatcgccat atcatggata tcggcaatct cagcgagcaa atcaccactg gcctgttcac
    11161 gacttaccgg agcggataat gtttccctga gggcgttgcg ttcctccgct aactgctgct
    11221 ttttttgccg tcgctcttgt tcaatctcct gtggataaaa cgcttcacgc aggctttgcc
    11281 tgacctgtaa aactgactca tccatctgct gctgcgcaag ccagatagcc ccggtcagaa
    11341 gaagactgcc cgcaacaata atcccgcctt ccgtaacgag tcggttttgc ccagataaac
    11401 gcgcgctcag ccaacccaac gcgccacccg caatcaggcc gccaatgtgg caagcgttat
    11461 ctattcctgt ttgccgggca ccgtacaaca gtgtcagcgc caccataccc aacagaggaa
    11521 atactcgccg ctggttttta tggtatgtgc ccgtacctga agcaaactgt gttgctatcg
    11581 ctgcaccagc tattcccaga atcgccccgg atgccccaac gctgatgact atctgctcac
    11641 tttcgcgtaa cgcataacag gcgctgatga gcccaccaaa gacgccggag aataaccagg
    11701 taatcaggag tcgccatttc cccagaatct gttccgccac gcctcccacg accaggaatg
    11761 caaacatatt catcagcaga tgagaaacac tactgtgcag aaagatactg ctcaataagc
    11821 gccagggttc acccgacaaa gtaagaggcg caacgtttgc gccgagatgg ataagtgcgg
    11881 attgatcgac ctcttcataa ctcatccatt gtgcgagata acaaaatatc ccaatacaga
    11941 gtgcaaaaag gataagggtt attgcgggta attgaacatt taacggctta accgaagatg
    12001 cagacataag gtgtttcttc cttgaaaaga caggtaaaaa gcatttttcc cgtgtgcagt
    12061 tatcatcccg gcaaacgtcc catgccgaaa agataattct gtccgaaata tgactttttc
    12121 agcctaaaat tacaatcgta attaattcgc ccctaagtat tttttggcat gtaagttgct
    12181 ttatttactg tgtaagcgat gaaatcagcc gcgacgcaac caggttgcac caccacaaat
    12241 cagcggtagc accaaaaagg tgcggtttaa gaattttgct tcggaatggt gcggcgcatc
    12301 tcgcaaaaac aaacaataaa acagcaatat ctttacattt agccaaacta aatgttacct
    12361 aacaggcaca acactgcaca ataaagttgc agacgataac aacacaaaca ctcacaacgg
    12421 gtatccatgc gttcttaacg cagaagataa aggagttgga tatgcaatta cgtaaacctg
    12481 ccacagcaat cctcgccctg gcgctttccg caggactggc acaggcagat gacgccgccc
    12541 cggcagcagg cagtacgctg gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc
    12601 gtgaatcttc agtacctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc
    12661 aggattactc caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc
    12721 aggtaaaact gattccgatt acctcacaaa accgtattcc actgctgcaa aacggcactt
    12781 tcgattttga atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct
    12841 ctgatactat tttcgtggtc ggtacgcgcc tgctgaccaa aaagggcggc gatatcaaag
    12901 attttgccga cctgaaagac aaagccgtag tcgtcacttc cggcactacc tctgaagttt
    12961 tgctcaacaa actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc
    13021 acagtgactc tttccgcacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgatg
    13081 ccctgctggc cggtgaacgt gcgaaagcga agaaaccaga caactgggaa atcgtcggca
    13141 agccgcagtc tcaggaagcc tacggttgca tgttgcgtaa agacgatccg cagttcaaaa
    13201 agctgatgga tgacaccacc gcgcaggtgc agacctccgg tgaagcggaa aaatggtttg
    13261 ataaatggtt caaaaatccc attccgccga aaaacctgaa catgaatttc gaactgtcag
    13321 acgaaatgaa agcactgttc aaagaaccga atgacaaggc actgaactaa ttacaagaac
    13381 caggggcgga aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctga
    13441 tggtcgttcc ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag
    13501 cagttaacgc tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg
    13561 ggtatttttt tacaacaagc cccgttcggc aacaccacct atcttggttg gatctggagt
    13621 ggttttcagg tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt
    13681 tcatttttcg gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg
    13741 tatgtcgaac tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtatctggtg
    13801 atcccggagc tgttgccgga gaaaatcggc atgtggttta aggccgagct ggatcccaat
    13861 attcagtttt tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc
    13921 gaacaggtac gcgccgccat tcagtcgttg ccgcgtgggc aaaaaaatgc cgcgctggcg
    13981 atgggcctga cgctaccgca ggcttatcga tacgtactgc tgcctaatgc ttatcgcgtt
    14041 atcgtcccgc cgatgacttc cgagatgatg aacctggtga aaaactccgc catcgcctcg
    14101 actattggtc tggtggatat ggcggcgcag gcgggtaaat tgcttgatta ctcagcccac
    14161 gcctgggagt cgtttaccgc tattacgctg gcgtatgtgt tgattaacgc ggttatcatg
    14221 ctggtaatga cgctggttga acgtaaagtt cgcctgcctg gcaacatggg gggcaaataa
    14281 tgtacgagtt tgactggagt tccattgtcc cttccctgcc atatctgctc gacgggctgg
    14341 tgatcacgct gaaaatcacc gtcacggcag tcgtgattgg tattttgtgg ggcaccatgc
    14401 tggcggtgat gcgcttgtcc agctttgcgc ccgtagcgtg gtttgccaaa gcctacgtta
    14461 acgtattccg ctcgattcct ttagttatgg ttttgctgtg gttttacctg atcgtgccag
    14521 gttttctgca aaacgtgctg ggattatcac caaaaaatga tatccgttta atttcggcga
    14581 tggtggcgtt ttcgatgttt gaagcggcct actattcaga gattatccgt gctggtattc
    14641 aaagtatttc tcgtggtcag tcgagcgccg cgctggcgtt gggaatgact cactggcagt
    14701 cgatgaaact gattattctg ccgcaggcgt tccgcgcgat ggtgccgctt ctgctcactc
    14761 agggcatcgt actgttccag gatacctcac tggtgtatgt gttatctctg gccgatttct
    14821 tccgtaccgc ctcaaccatt ggtgagcgtg atggtacgca ggttgagatg atcctgtttg
    14881 ccggatttgt ttatttcgtt attagtctta gcgcgtcgtt gttggtcagc tacttgaaaa
    14941 gaaggacagc ataatgatta ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt
    15001 gctgaccgac tgctcaaccg aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc
    15061 tggttccggc aaatcaacgc tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg
    15121 tgaaatcacc gtcgatggta tcgtggttaa tgacaagaaa accgatctgg caaagctgcg
    15181 ttcccgcgtc gggatggtat tccagcattt cgagctgttc cctcatctgt cgattatcga
    15241 aaacctgacc ctggcgcagg tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa
    15301 agcattgaaa ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca
    15361 gctttccggc ggtcagcagc agcgtgtggc aatcgctcgc gcattgtgta tggatcctat
    15421 tgcgatgctg ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct
    15481 ggacgtgatg gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat
    15541 gggctttgcc cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga
    15601 agactcgccg aaagacgctt tcttcgatga tccgaaatcg gaccgcgcga aagacttcct
    15661 cgcgaaaatc ctgcattaat cactctggcg cgcataccca agtgcgcgcc atctcacgct
    15721 ttatcgtttc gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac
    15781 acaacaagga gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgacga
    15841 cgctatcgca atagttctcg ccctcgcctc accagagctt gatgtcaaag caattacgtc
    15901 ttccgccgga aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt
    15961 gcttaatcgc accgatattc cggtagcagg cggcgcggta aaaccgttaa tgcgtgagtt
    16021 gattatcgcg gacaatgtgc acggcgaaag cggtctggat ggcccggcat taccggaacc
    16081 gacgttcgca ccgcaaaact gtacggcggt agagctgatg gcgaaaacgc tgcgcgaaag
    16141 tgcggaacct gtcacgattg tgtctaccgg accgcaaact aacgttgcct tgctgctcaa
    16201 tagccatccg gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg
    16261 gcttggtaac tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga
    16321 aattgtcttc cagtcaggga tcccagtggt gatggccggt ctggatgtta ctcacaaagc
    16381 acaaatccac gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat
    16441 tgttgccgaa ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt
    16501 cggtgcaccg ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac
    16561 cactgttgag cgctgggttg gcgtggaaac acaggggaaa tatagccagg gtatgacggt
    16621 tgttgattat tattatctga ctggcaataa accgaatgcc actgtaatgg tcgatgttga
    16681 tcgtcagggc tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacacacgtc
    16741 attgattaag atcaccacgc gttacctggg tggtcttaat ctgtttatct gtttatctgt
    16801 ttatctgttt atctcttttt ctttcaagat ataaatgcaa tggataatgc agaactcgcc
    16861 attggtatcg atctcggcac taccaatagt ttaattgccg tctggaaaga cggtgccgcg
    16921 caattaattc caaataagtt cggtgaatat ttaacaccat ccataattag catggatgaa
    16981 aataatcata ttttagtcgg aaaaccggct gtatcacggc gtacttcgca tccggataaa
    17041 acggcagcgt tatttaaacg tgcaatgggc agtaatacca actggcggtt aggcagcgac
    17101 acatttaacg cgccagaact gtcctctttg gtattacgct cattaaaaga agatgccgaa
    17161 gaatttctgc aacgtccgat taaagatgtg gtgatctccg ttccggctta tttcagcgat
    17221 gaacaacgca agcatacccg tttagcagcg gagttagccg ggttaaatgc ggtacgctta
    17281 attaatgaac ccacagcagc tgcgatggca tatggcctgc atacgcaaca aaatacccgc
    17341 tcgctggttt ttgatctcgg tggcggcacg tttgacgtca cggtgctcga atacgccacg
    17401 ccggtgattg aagttcacgc ctccgctggc gataacttcc tcggtggcga agattttacc
    17461 catatgttgg tcgatgacgt tttaaagcgc gcggatgtcg ccaggaccac gcttaacgag
    17521 agtgaactgg cagccttgta cgcctgtgtg gaagcggcaa aatgtagcaa tcaatcgcca
    17581 ttgcacattc gctggcagta ccaggatgaa acgcgggagt gcgaatttta cgagaacgaa
    17641 ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag tgccgattga acaggcgttg
    17701 cgcgatgcac gtctgaagcc gagtcaaatc gacagtctgg tgctggttgg cggcgcgtca
    17761 caaatgccgc tggtgcagcg aatcgccgtg cgtctgtttg gcaaattacc gtatcaaagt
    17821 tacgacccga gcaccattgt cgcgctgggc gcagcaatcc aggccgcctg ccgcttacgc
    17881 agtgaagata ttgaagaggt gatcctcact gatatttgcc cttactcgtt gggcgttgaa
    17941 gttaaccgcc agggcgtttc cggcattttc tcgccaatta ttgaacgaaa caccacagta
    18001 cccgtgtcgc gtgtagaaac ttactcgacc atgcacccgg aacaggattc tattacggtt
    18061 aacgtctatc agggagaaaa ccacaaagtt aaaaataaca ttctggttga atccttcgat
    18121 gtgccgttga agaaaaccgg ggcttatcag tcgattgata ttcgctttag ttatgatatc
    18181 aacggcttgc ttgaagttga cgtgcttctg gaagatggca gcgttaagtc cagagtcatt
    18241 aatcatagcc cggtaacatt gagcgcgcag cagattgaag agagtcggac gcggttatcc
    18301 gcattgaaaa tttatccgcg cgatatgcta atcaaccgca cctttaaggc caaactggaa
    18361 gagttgtggg cgcgggcgct gggtgacgag cgagaagaga ttggccgggt gatcaccgat
    18421 tttgatgcgg cgttgcagtc aaacgatatg gcccgcgtcg atgaagttcg tcgacgggcg
    18481 agcgattatt tggctattga gatcccttaa acacggctgc cggatgcggc ataaacgctg
    18541 catccggcat cggcgcaaaa aacacatcct cagctaaaca acatcgcgat ttttccgagc
    18601 gcataaccta tcatcagcac cgttaagctg attttgataa tactggtaat gctctgtggt
    18661 ttttcatcct catcattttt ttcttcatgg ttttcaggtg ttggctcatc gacatcttca
    18721 tcctccggct cgagtttcac tttcgtcaac agcatctggg tgataatggc ctctaatgga
    18781 tgagcatctc caccttccag ccttgtatta agacgggcca gcatctcttg tttcgggaac
    18841 gcttccagca caacctgttc cgctacccga tacaaatcca gtaacggtga atcttgctct
    18901 gtctcgctac cgactctcga tttccaccac gcccatgctt tgcgcttgaa gaataacgac
    18961 tcaataaacg attgacctaa cggcgataat tttccgctat gtaaaatctc cgcccataac
    19021 agccgcgtcc gggcgctctc accaggaaca tatttcaacg gtttaccttg cgcatctacc
    19081 atgcttcgcg cttcgataac catcactaat aacgcgaaat aatccgggca atgacgcttg
    19141 ctccactgca acaacaaatt atcagcttgt gtagatgggt aactttccca gtacgcgcag
    19201 agatcggcaa gcaggctttc cccctcgccg cagtacaaac gttgtgcaca ctggtaatac
    19261 cgggcatctt cgctttctgg ttcttccgct tgccactgtt tggctacagg aatgagttcc
    19321 gccatacctg tttgcaccga gctgtaccag cgcaacagtt tgcgatgtaa ctttgcatca
    19381 tcaggaatga accacggtcc ttccatcgcc aaaaacgcag tgacatagtc aggatggtaa
    19441 cggcagcaac gttccagcgc gaagtaatat tcaacagtct ggttctgtaa cgcgacggga
    19501 tattccagaa ggctaaggaa atcgaaacaa tcaccagcct gaatggcctc aagaaaagcc
    19561 tccctttctt cctcatccat tccttcgctg tcattaaacg aatgccagtt caaacgctct
    19621 gccaacaaag aagcacaatt taatgaaaga tatcgcgctt ctatagcgat tgcacacagc
    19681 ggccagcgta attgatcgac atcctccatg ttccaggtat ttaattgctg aataaatttc
    19741 tgccaggcgg agggctgaaa acgatcactt tctgaatcca gtaatgtcct gaatgcacgt
    19801 agtatttcat gttcagcggt ggcatctttt tcttcatcat ctgcttcctc gacaggattt
    19861 accgccagtc gcagcgcctc ttcataggcc tggcgtaatg ctttaaaccc ctgcggatcg
    19921 gtttcgggat gacacaacgg taagcgagca agataagcct ggcggataat gtctatttgc
    19981 gtcgtgcttt caatttcgag gatttgccaa catgttttca taggtcgctc acatccttaa
    20041 gcgttaccgg gcattcaggc tggacagtta atggttgcca gggaagcgtg gcatagaaac
    20101 ggtcgcttcc cgctgccagc tcttcgaaca tccgactccc ggcgctatcg tatttgtaca
    20161 gggttagcgc ctcgcgtagc gaagcgttgt cttcgcactg agaagattgc cagtagagac
    20221 gccctagtga ataggcagaa aaatagtgca tccagctgtg atagtaatgg tgagctcgtg
    20281 catagacgcg cgattgcagc cataaactct cttcttccgt cagccagttg ttgagcacac
    20341 cgacccgtga cagaaacccc attcgaacat aatcccaggc tttgattccc ccttctccac
    20401 agcacatagc ggtttgggca acgaattggg tataaatcaa ccctctctcc gagccaccag
    20461 caattaatgc tttccactct tcaggggaag agcgttgcca tgtcagatat aagccagcta
    20521 aatcattggc atgaccatca tcagtcatct gataaatagt ctgaattaaa tcatcacgat
    20581 tatttattcc ccaggatgat gagagatcga attcttttat cccagtatac atttttggcg
    20641 agtcatgcgc gggtttgcaa ctctgattaa gtatttccat gggcgcagaa agcgcaaaaa
    20701 gaagatattg ttcttcctta ttcatttttt aataatccct taagtgcatt aactgactac
    20761 ttttttatta ttgatgagta taaacttgac gctttcaaaa taaaaagaaa atcgaagcat
    20821 tcacacatga ataaaaaatc aatgtatata ttcgcaattt ttatagttgc agcaattacc
    20881 tgtattagcc aacccaagaa aacgacgttg cgtgataaag ccatggtgaa ttatgccttt
    20941 gattatttaa gatcaccagg cagtctaccg ttaaccacgg cagccacgga gctttccgcg
    21001 attcatggtc actcaacatc gcaatatcgc cttggcgaat tttatcttca tggtagcgac
    21061 ggtaaaccac tggattatac acaggcgaga tactggtatg agcaatcagc agaacaggaa
    21121 aatccgcgcg cacaaagcaa actggggtgg atctacctga aaggcctggg agtcaaaccc
    21181 gatacccgta aagcaattct ctggtataag gaagcagctg aacaagggta tgctcatgct
    21241 caatatactt tgggtttgat ctacagaaat ggcactggta ttaatgttaa ccattatgaa
    21301 tctcaaaaat ggttaaaact ggccgccaaa caacattaca aaaatgcgga aagattactt
    21361 gccggacttc ccgcacatta acgtcgactg tcacccgaag agatggagta tttttcctat
    21421 caaaccggca aagataaaaa tgtagaaaat aattttgatg acgcccagac agcccagccc
    21481 gccatcgtca acgggcattg gctgcggttg cggctccggc tcgacggttt cctgaccggt
    21541 aaaattcgcc gcctgaatta ataatcgacg aataattaat gcttccagtg gacaacgatc
    21601 ttctgcatca aggcggctca caatcggcat taatgattct tctgtcggtt ggctggcctg
    21661 cactaattgt ttggcaacta aatataatgg ccataaggga tgttctgtat cacgcgtgag
    21721 tgagatccgc ggcttacgtc ctttgtgcaa aaccatctcg accagtgaac gccccagcgc
    21781 agagagtttt tcatcgctga gtatttcgta taaatgaaaa cgcgtacggg cgctgtcgcc
    21841 ggggacataa agtagctgtt taccctgatc gttaaccagt tcacgagctt caatcatcat
    21901 caccactaac gggtaataat cgacccgatg ctgacggcac cattgcaata ttaaagcatc
    21961 cgcctgggtg gagggatact cgcgccagta gtcacaaagt tctgccagca agctttcgcc
    22021 ttctccacaa tagacgcgtt gcgcgtattc ataataacgc gcatcttcat tatcaggttc
    22081 agcttcccgc cacgaccggg cgtaatccag aagttcgggg atatccaggc gacctgcgct
    22141 ataccagcgg agtaaatttc tgtgcagttt tgcatcatcg gggatgatca ctgcgcgatg
    22201 ttgcgctaaa tacaatgtca gccattctgg atgcgatgac caaatccgag cgaacatttg
    22261 gtaaaaatcg atggtgtcat tttgcacggc aacgggcaga tgcagaatgg tctggaagtt
    22321 aaaaacattc cccttcgcaa tggcatataa aaaggattcc agttcttctt cgtctatttc
    22381 cccgacgttt tcctcctgca accaccgcaa tctttctgct aataacacca cgcactcgaa
    22441 ggataaatgg gcagtgttca tggcgattgt gcacagcgac cagcgtaatt catcaatctc
    22501 ttccatcgag caataattta attgctgaat gaatcgctgc caggcggagg gcagaaaacg
    22561 ttcgctatca gaggcaagta acgcgcgaaa ggcgagcaga atttcatgtt ctgctacctc
    22621 atattcttct ggttgccaaa cagatttagc aggcgactgc gcaatccgga gcgcctcctc
    22681 atacgcctga cgaagttgtt taaaaccctg cggatcggtt tctggatgaa aggacggtaa
    22741 cagcgccaga taagcgcggc ggataatatc gacgtcagtc gtttcctcta tttcgaggat
    22801 cttccagcaa tttttcatag gtcgctcata tccttaagcg tttccgggca tgtggggtag
    22861 tcagccagtg gttgccagga caaggcaggg taatacgcat catcactggc aaataattga
    22921 taaaacatat cattgccgga atcgtcgtat tctttacgag ctaagaattc gcgtaataaa
    22981 ggcagatgat catcttcggg agactgccaa tattgccgac caaaggtata accggcaaag
    23041 tattgccgcc agttactata ataacgtaga gcccgtagat gaatacgcga ctggatccat
    23101 agactttctt cttcactcaa ccagttatta agtacgccca ttcgacttaa aaaacccatc
    23161 cggacataat cccatgcttt aattcctccc tcaccacagc attcggctgt gctggcgaca
    23221 aattgcgcat aagcctgacc ttgctcattt agttcagcaa gatagtcacg ccattcacat
    23281 ggcgaataac gaaaccagcg gtgataaaac cctgccaacc gagcggcatg accgttatcg
    23341 atcattcggt gaattgtgcg cagcaggtca tcacggtcat caattcccca tgaggaagaa
    23401 aggtcaaaaa agcatgtacc taaatacatt ttgggggatg aatgcgaagg taaacagcat
    23461 tcattgcgta tttccatcgg agtggaaagt gcaaacaata ttttttgcga ccccatgtcc
    23521 atataattta tccttaaaca ttcatttaat gaatgtaaac tacatttcga gactcagtta
    23581 tttatacttt tctgacgcaa cataatacgc ataaaataag gcttacgcaa aaatggaaca
    23641 cataaaaata taatatgttt tatctacaac cataaggaaa tgataatggt ttttacgtca
    23701 agttgctgcg ataatttatc aatagatgag attatcgaac gtgctgaaaa aggcgattgc
    23761 gaggctcaat atattgttgg gttttattat aatcgcgata gcgcaattga ctctccggac
    23821 gacgaaaagg ccttttactg gctgaagctg gccgctgagc aaggtcattg tgaagcacag
    23881 tattccttag ggcaaaagta taccgaggat aaaagccgtc ataaagataa tgagcaagcc
    23941 atcttctggc tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa cgcccttggc
    24001 tggacactgg atcgtggaga agcccccaat tataaagaag cggttgtctg gtatcagata
    24061 gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg ggtggatgta cagaaatggc
    24121 aacggagtcg caaaagacta tgcgctggca tttttctggt acaaacaagc tgcattacaa
    24181 ggccatagtg acgcgcaaaa caatctggcc gatctttatg aagacggaaa aggcgttgct
    24241 caaaacaaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg taatcggcac
    24301 gcccagtttc aaattgcatg ggattataac gctggcgaag gggtggacca ggactataag
    24361 caagcgatgt actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc ttacgtcaac
    24421 atcggttata tgtataaaca cggacaaggc gttgagaaag attatcaggc tgcctttgaa
    24481 tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct ggccattatg
    24541 tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct gtatcgtaaa
    24601 gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga agatttactg
    24661 tagagataaa aacaagggcc ggggaatttc attcggccca tattctcttt attgctgtgc
    24721 gaaatcagac tattacggct caaacgggcg tctctggaac tggtcatgac cacatttcgg
    24781 gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt tctcgcagac
    24841 cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat tgaggtcctg
    24901 gaaaacttcg cgccattcaa gctgcgtttt atcggtgata tctgccagct cctgccataa
    24961 gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc tctcttcata
    25021 gctcatggcg aactcttcca ggtcacgtct tacagctcgc gtcagctcat cgacctcggt
    25081 tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca gtgcgtcgat
    25141 atcacgttcg ccattgcgca ggcgttcgct cagtgacgcg accagttcac ggtaatattg
    25201 agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata atagacgttc
    25261 tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtat gaataatccg
    25321 ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt tgggctatgc
    25381 tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa aacaggacca
    25441 ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt acagcttcat
    25501 tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa gtattactgc
    25561 ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg taactacacc
    25621 atcggtgacg tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct gcagccgatc
    25681 ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa caccgctccg
    25741 gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat gctgggcttt
    25801 ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg ttgggaacag
    25861 aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc tgcggtcaac
    25921 tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg ctgctgctgg
    25981 cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa aatcactgct
    26041 tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga caccgttaaa
    26101 accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt caacgttaac
    26161 gactatgaca acatgctgac cgtttacact acccgcccgg acacctttat gggttgtacc
    26221 tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa taatcctgaa
    26281 ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga aatggcgacg
    26341 atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac gggcgaagaa
    26401 attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc agttatggcg
    26461 gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct gaacatcaaa
    26521 ccagttatcc tggctgctga cggctctgag ccagatcttt ctcagcaagc cctgactgaa
    26581 aaaggcgtgc tgttcaactc tggcgaattc aacggtcttg accatgaagc ggccttcaac
    26641 gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa ctaccgcctg
    26701 cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat ggtgacgctg
    26761 gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct gccggaagat
    26821 gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc aaaaactacc
    26881 gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat ggagtcctcc
    26941 tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga ttccgaagcg
    27001 gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc cattatgcac
    27061 ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt gaactctgac
    27121 gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt ctactatgtt
    27181 ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga acgtgacgag
    27241 aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta taccggcatg
    27301 agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt tgaacgttac
    27361 ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat gactctcgaa
    27421 tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg gaaactggtt
    27481 tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct gactgaagat
    27541 cagaaagcgc tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga tgatatcggc
    27601 cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa caaactggcg
    27661 aaagcaccaa ccgatggcga gcaggatcgc gctctgatgc aggaagcgct gctggccgtt
    27721 gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca ggaactgaaa
    27781 ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc gatggtggaa
    27841 gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat caccgttctg
    27901 gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct ggtagcaaaa
    27961 tatcttgatg gcgttaccgt gcgtaaagtg atttacgtac caggtaaact cctcaatctg
    28021 gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg ttatctctgg
    28081 cggtgttaat caccgccggg tgtggctggc atctgcgtga taccacgcag gttccttcca
    28141 ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc cgtgcagtgc
    28201 gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg cgtaaggacg
    28261 ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg gtattccgta
    28321 acggtcaaac agcagagtat cagatgatca tgacggtgaa tgcgaccgtg ttgatccccg
    28381 gccgtgatat ctacccgatt agcgccaaag tcttccgttc gttctttgat aacccgcaaa
    28441 tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac gaccgtgctg
    28501 ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt tccgacgaag
    28561 aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc accacgctgg
    28621 gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg aagggctgcg
    28681 cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc aggacgctgt
    28741 tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca ttgatcccaa
    28801 cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg ccagtcgaca
    28861 aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg aacaacttct
    28921 cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta ataaattaag
    28981 caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg tgcaggtgac
    29041 ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg caaaacagct
    29101 caacttagaa ctggatgacg cggcaaatca ggtgctctgc tactgttatg aaggtaacct
    29161 gctggcgctg gctcaggcac tggagcgttt atcgctgctc tggccagacg gcaaattgac
    29221 attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt ttcattgggt
    29281 tgatgctttg ttgatgggaa aaagtaagcg cgcattgcat attcttcagc aactgcgtct
    29341 ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactgt tgttactggt
    29401 taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata agcatcgggt
    29461 atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc agacgcagtt
    29521 acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag attacggtca
    29581 gtcagtgtgg gcagagctga aagggttatc tcttctgttg tgccataaac ccctggcgga
    29641 cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc acctttgatc
    29701 cggtgcacta tggtcatcta aaacccgtgg aaacgctggc gaatttgatt ggtctgacgc
    29761 gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa gcgaacagcg
    29821 tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt actcttgatg
    29881 aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa gagtggcggc
    29941 aggaacaagg accggacgtg ccgctggcgt ttattattgg tcaggattca ctgctgacct
    30001 ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc gtctgtcggc
    30061 gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg gaagatcatt
    30121 tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat ctggctgaaa
    30181 cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac ggtgaatcgt
    30241 gtgaggattt attgccggaa ccggtactga cttacattaa ccaacaaggc ttgtatcgct
    30301 gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg gtgaaacgca
    30361 agcgaatatc gatggtcttt acagcggtca tgcgcccacc cccctgaccg cgcgcggtat
    30421 tgagcaagcg caaaatctgc atacgctgct acatggtgtt tcctttgatc tggttttatg
    30481 cagtgaactg gaacgggcac agcataccgc acgactggtt ctcagtgacc gccagctccc
    30541 cgtgcaaatc atccctgaac tcaacgaaat gttttttggc gactgggaga tgcgacatca
    30601 tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg actggcagca
    30661 tgcaatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg aacgctttat
    30721 cgcaaggctt agtgaatttc agcactatca gaatatttta gtcgtcagcc atcagggtgt
    30781 actgagtctg ttaatcgccc gtttaattgg catgcctgcc gaggctatgt ggcattttcg
    30841 cgttgactag gggtgttgga gcgccattga tatcaaccaa aaattcgcga cgctacgcgt
    30901 cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt ttacgggtaa
    30961 gccgcaacga ccattgacag acccgggcag gctgatattc tccgcagcca gacttttttc
    31021 gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct gcggcgcggg
    31081 gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga aatcgcctct
    31141 ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg gaaaacttgc
    31201 agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa ggtcaggaca
    31261 tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga ctgcatgatc atctgtacgg
    31321 gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag tctcgcgcag
    31381 cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt gtcgtggatt
    31441 tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat gaactggaaa
    31501 aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa atgccggact
    31561 gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg cccttcgagc
    31621 tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc atactcgaca
    31681 aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc ctcgatattc
    31741 caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg aagctggatg
    31801 gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc tgtaaagcgg
    31861 cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg gttcgcgtgc
    31921 tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct tatcaccgtg
    31981 agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac tcttttcgcg
    32041 actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt ttggggattt
    32101 tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt cgctttaccg
    32161 actaccagac tcgctctaat gaaaaccgca ttaagctggt gcctatcgcg cccagccgcg
    32221 gcattatcta cgaccgtaat ggtatccctc tggccctcaa ccgcactatc taccagatag
    32281 aaatgatgcc agagaaagtc gataacgtgc agcaaacgct ggacgctttg cgcagcgtgg
    32341 ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt tcacaccgtt
    32401 tcacctctat tccggtgaaa actaacctga ccgaagtaca agtagctcgc tttgccgtca
    32461 atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac tatccttacg
    32521 gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa gacgtcgaac
    32581 gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc ggtaagctgg
    32641 gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa gaggttgaag
    32701 ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa gccggacacg
    32761 atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg ctggcgggta
    32821 gccgcgcagc tgtggttgtc accgatccgc gtacaggtgg ggtgctggcg ctggtttcca
    32881 cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat tattccgcct
    32941 tgttgaacgc cccgaataca ccgctggtga accgcgccac acagggggtt tatcctcccg
    33001 cgtctacagt taaaccctat gtggcggttt cggcattgag cgccggggtg atcacgcgca
    33061 atacgacgct gtttgaccca ggctggtggc aactgccagg ttcggaaaaa cgttatcgtg
    33121 actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa gaatctgcgg
    33181 ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc gaatggatgg
    33241 gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt tccggcaaca
    33301 tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag ggtgacacca
    33361 ttccggttgg tatcggtcag ggttactgga cagcgacccc aatccagatg agtaaggcac
    33421 tgatgatcct gattaatgac ggtatcgtga aggttcctca tttgctgatg agcaccgccg
    33481 aagacggcaa acaggtgcca tgggtacagc cgcatgaacc gcccgtcggc gatattcatt
    33541 ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc cctaacggta
    33601 cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatcc ggtaccgctc
    33661 aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc gagcgtttac
    33721 gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg gctgtcgcca
    33781 tgattctgga gaacggtggt gcgggtccgg cggttggtac actgatgcgc cagatcctcg
    33841 accacattat gctgggtgat aacaacaccg atctgcctgc ggaaaatcca gcggttgccg
    33901 cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct gggataaagt
    33961 ccatctcgat cccacaatgc tgctgatctt actggcattg ctggtttaca gcgccctggt
    34021 tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatcg gccaaatcgc
    34081 gatgggtcta gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt atgaaggctg
    34141 ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg ctttcggtgc
    34201 catctctaaa ggtgctcaac gctggctgga cctcggtatt gttcgttttc agccgtcgga
    34261 aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg acgtttgccc
    34321 gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca cgctgctggt
    34381 ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc tgtttgtact
    34441 gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg tggcggcgtt
    34501 cattccgatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg taatgatgct
    34561 cctagacccg gaatcagacc cactcggcgc gggctatcac attattcagt ctaaaattgc
    34621 tattggctcc ggcggattac gcggcaaagg ctggctgcac ggcactcagt cacagcttga
    34681 atttctcccc gaacgccata ccgactttat cttcgcggta ctggcggaag agctgggatt
    34741 agtgggcatt ctgattctgc tcgctctcta cattctgctg atcatgcgcg ggctgtggat
    34801 agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggtggcttaa tgctgatatt
    34861 attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg ttgtaggggt
    34921 tccgctccca ctggtcagtt atggaggatc ggcgctaatt gtgctgatgg ctgggttcgg
    34981 gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt aagaggtgcg
    35041 caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg gcatgtacaa
    35101 gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt aacggcccca
    35161 tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg gcaaatcagg
    35221 attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga tttagccagg
    35281 cgggactggc ggcaatctat gatgccgaac ccggcagtaa cctgacggcc tctggcgaag
    35341 ctttcgatcc gacacagctg acggcggccc atccaacgct tccgatcccc agctacgcca
    35401 gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat cgcggccctt
    35461 acggcaacga ccgcgttatt tcactttctc gcgcggcagc tgaccgtctt aacacgtcaa
    35521 acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg ctttctggtc
    35581 ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgccagca cctcccgatt
    35641 taagcggtgg cgcgggaaca agttcagtgt ctggcccgca gggtgacatt cttccggtca
    35701 gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt agcggtttcc
    35761 tcggcgctcc aacgacctta gcgcctggtg tactggaagg cagcgaaccg acgcctgcgc
    35821 cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca atggtgacac
    35881 cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg gccgtaagcg
    35941 atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc gtccccggtc
    36001 gcgtaactca aaatggcgcg gtctggcgga tccagcttgg cccattcgcc agcaaagccg
    36061 aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca tttattacta
    36121 ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga cgttaacaaa
    36181 gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg cactttttta
    36241 attccatcac ggatgtcgta gttcagacca tgaataccat tttttccgct cgtatcatga
    36301 agcgcctggc gctcaccacg gctctttgca cagcctttat ctctgctgca catgccgatg
    36361 acctgaatat caaaactatg atcccgggtg taccgcagat cgatgcggag tcctacatcc
    36421 tgattgacta taactccggc aaagtgctcg ccgaacagaa cgcagatgtc cgccgcgatc
    36481 ctgccagcct gaccaaaatg atgaccagtt acgttatcgg ccaggcaatg aaagccggta
    36541 aatttaaaga aactgattta gtcactatcg gcaacgacgc atgggccacc ggtaacccgg
    36601 tgtttaaagg ttcttcgctg atgttcctca aaccgggcat gcaggttccg gtttctcagc
    36661 tgatccgcgg tattaacctg caatcgggta acgatgcttg tgtcgccatg gccgattttg
    36721 ccgctggtag ccaggacgct tttgttggct tgatgaacag ctacgttaac gcactgggcc
    36781 tgaaaaatac ccacttccag acggtacatg gtctggatgc tgatggtcag tacagctccg
    36841 cgcgagatat ggcgctgatc ggccaggcat tgatccgtga cgtaccgaat gaatactcga
    36901 tctataaaga aaaagaattt acgtttaacg gtattcgcca gctgaaccgt aacggcctgt
    36961 tatgggataa cagcctgaat gtcgacggca tcaaaaccgg acacactgac aaagcaggtt
    37021 acaaccttgt tgcttctgcg actgaaggcc agatgcgctt gatttctgcg gtgatgggcg
    37081 gacgtacttt taaaggccgt gaagccgaaa gtaaaaaact gctaacctgg ggcttccgtt
    37141 tcttcgaaac cgttaaccca ctgaaagtag gtaaagagtt cgcctctgaa ccggtttggt
    37201 ttggtgattc tgatcgcgct tcgttagggg ttgataaaga cgtgtacctg accattccac
    37261 gtggtcgcat gaaagatctg aaagccagct atgtgctgaa cagcagtgaa ttgcatgcgc
    37321 cgctgcaaaa gaatcaggtc gtcggaacta tcaacttcca gcttgatggc aaaacgatcg
    37381 agcaacgccc gctggttgtg ttgcaagaaa tcccggaagg taacttcttc ggcaaaatca
    37441 ttgattacat taaattaatg ttccatcact ggtttggtta aaaattgaac acttgaaagt
    37501 gtaatttccg tccccatata ctaagcatca gtaaaaaact cccgccttct ggcgggagtt
    37561 gctatttaat tacgttacgc cggagctgac atgaaaacca aacttaacga actgcttgaa
    37621 ttccctactc cttttactta caaagttatg gggcaggcgt tacctgagct ggttgatcag
    37681 gtggttgaag tggtacagcg ccatgcgcca ggtgactaca ccccaacggt aaaaccaagc
    37741 agcaaaggca actaccactc ggtatctatc actatcaacg ccactcatat cgagcaggtt
    37801 gaaacactgt atgaagaact aggcaaaatc gatattgtcc gcatggttct gtaactcgct
    37861 tctccgttac ccggcccctg gtcgggtaac tcccctctcc tcgctgtgat atacttttcc
    37921 cccactttta ctcattctcc acggagatgc agttttgtat caggataaaa ttcttgtccg
    37981 ccagctcggt cttcagcctt acgagccaat ctcccaggct atgcatgaat tcaccgatac
    38041 ccgcgatgat agtacccttg atgaaatctg gctggtcgag cactatccgg tattcaccca
    38101 aggtcaggca ggaaaagcgg agcacatttt aatgccgggt gatattccgg tgatccagag
    38161 cgatcgcggt gggcaggtga cttatcacgg gccggggcaa caggtgatgt atgtgttgct
    38221 taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg agcaaacagt
    38281 ggtgaatacc ctggctgaac tgggtataga agcgcatcct cgggctgacg cgcctggtgt
    38341 ctatgtcggg gaaaagaaaa tttgctcact gggtttacgt attcgacgcg gttgttcatt
    38401 ccacggtctg gcattaaacg tcaatatgga tctttcacca tttttacgta ttaatccttg
    38461 tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg aaacccgaag cgacgactaa
    38521 taatattgca ccacgtttac tggaaaatat tttagcgcta ctaaacaatc ctgacttcga
    38581 atatattacc gcttaattgc atacctcaat ggcccaattt acactgggtc attactcata
    38641 atacctgcta atgctttaca tctccttcgc gtttgtatac tttctttatc acttgcagaa
    38701 atgcggtgct acgccttgct ccgtaactca ccccattgat taacctattg aaatagttga
    38761 ttatggtgag caaaagcgtt ccgttccaat
//
LOCUS       DAZPBM010000021        38069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPBM010000021 DAZPBM010000000
VERSION     DAZPBM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.021"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(334..477)
                     /gene="ypaB"
                     /locus_tag="ACHUZQ_000980"
     CDS             complement(334..477)
                     /gene="ypaB"
                     /locus_tag="ACHUZQ_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1024740.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            605..2890
                     /gene="nrdA"
                     /locus_tag="ACHUZQ_000981"
     CDS             605..2890
                     /gene="nrdA"
                     /locus_tag="ACHUZQ_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1024741.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3124..4254
                     /gene="nrdB"
                     /locus_tag="ACHUZQ_000982"
     CDS             3124..4254
                     /gene="nrdB"
                     /locus_tag="ACHUZQ_000982"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1024742.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4254..4508
                     /gene="yfaE"
                     /locus_tag="ACHUZQ_000983"
     CDS             4254..4508
                     /gene="yfaE"
                     /locus_tag="ACHUZQ_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1024743.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4562..5211)
                     /gene="inaA"
                     /locus_tag="ACHUZQ_000984"
                     /pseudo
     CDS             complement(4562..5211)
                     /gene="inaA"
                     /locus_tag="ACHUZQ_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(5291..5395)
                     /locus_tag="ACHUZQ_000985"
                     /pseudo
     CDS             complement(5291..5395)
                     /locus_tag="ACHUZQ_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5390..5632
                     /locus_tag="ACHUZQ_000986"
                     /pseudo
     CDS             5390..5632
                     /locus_tag="ACHUZQ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5674..6750)
                     /gene="glpQ"
                     /locus_tag="ACHUZQ_000987"
     CDS             complement(5674..6750)
                     /gene="glpQ"
                     /locus_tag="ACHUZQ_000987"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1024744.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6755..8113)
                     /gene="glpT"
                     /locus_tag="ACHUZQ_000988"
     CDS             complement(6755..8113)
                     /gene="glpT"
                     /locus_tag="ACHUZQ_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1024745.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8386..10014
                     /gene="glpA"
                     /locus_tag="ACHUZQ_000989"
     CDS             8386..10014
                     /gene="glpA"
                     /locus_tag="ACHUZQ_000989"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1024746.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10004..11263
                     /gene="glpB"
                     /locus_tag="ACHUZQ_000990"
     CDS             10004..11263
                     /gene="glpB"
                     /locus_tag="ACHUZQ_000990"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1024747.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11260..12450
                     /gene="glpC"
                     /locus_tag="ACHUZQ_000991"
     CDS             11260..12450
                     /gene="glpC"
                     /locus_tag="ACHUZQ_000991"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1024748.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12643..13545
                     /locus_tag="ACHUZQ_000992"
     CDS             12643..13545
                     /locus_tag="ACHUZQ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1024749.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13586..14389)
                     /gene="yfaU"
                     /locus_tag="ACHUZQ_000993"
     CDS             complement(13586..14389)
                     /gene="yfaU"
                     /locus_tag="ACHUZQ_000993"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR1024750.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14407..15696)
                     /locus_tag="ACHUZQ_000994"
     CDS             complement(14407..15696)
                     /locus_tag="ACHUZQ_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1024751.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(15753..16958)
                     /gene="rhmD"
                     /locus_tag="ACHUZQ_000995"
     CDS             complement(15753..16958)
                     /gene="rhmD"
                     /locus_tag="ACHUZQ_000995"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR1024752.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(16973..17755)
                     /locus_tag="ACHUZQ_000996"
     CDS             complement(16973..17755)
                     /locus_tag="ACHUZQ_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR1024753.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(17975..19177)
                     /locus_tag="ACHUZQ_000997"
     CDS             complement(17975..19177)
                     /locus_tag="ACHUZQ_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1024754.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(19277..19819)
                     /locus_tag="ACHUZQ_000998"
     CDS             complement(19277..19819)
                     /locus_tag="ACHUZQ_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1024755.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            20098..20523
                     /gene="nudI"
                     /locus_tag="ACHUZQ_000999"
     CDS             20098..20523
                     /gene="nudI"
                     /locus_tag="ACHUZQ_000999"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1024756.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(20562..21164)
                     /gene="ais"
                     /locus_tag="ACHUZQ_001000"
     CDS             complement(20562..21164)
                     /gene="ais"
                     /locus_tag="ACHUZQ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1024757.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            21472..22611
                     /gene="arnB"
                     /locus_tag="ACHUZQ_001001"
     CDS             21472..22611
                     /gene="arnB"
                     /locus_tag="ACHUZQ_001001"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1024758.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            22615..23583
                     /gene="arnC"
                     /locus_tag="ACHUZQ_001002"
     CDS             22615..23583
                     /gene="arnC"
                     /locus_tag="ACHUZQ_001002"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1024759.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            23583..25565
                     /gene="arnA"
                     /locus_tag="ACHUZQ_001003"
     CDS             23583..25565
                     /gene="arnA"
                     /locus_tag="ACHUZQ_001003"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR1024760.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            25562..26452
                     /gene="arnD"
                     /locus_tag="ACHUZQ_001004"
     CDS             25562..26452
                     /gene="arnD"
                     /locus_tag="ACHUZQ_001004"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1024761.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            26452..28104
                     /gene="arnT"
                     /locus_tag="ACHUZQ_001005"
     CDS             26452..28104
                     /gene="arnT"
                     /locus_tag="ACHUZQ_001005"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1024762.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            28101..28436
                     /gene="arnE"
                     /locus_tag="ACHUZQ_001006"
     CDS             28101..28436
                     /gene="arnE"
                     /locus_tag="ACHUZQ_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1024763.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            28436..28822
                     /gene="arnF"
                     /locus_tag="ACHUZQ_001007"
     CDS             28436..28822
                     /gene="arnF"
                     /locus_tag="ACHUZQ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1024764.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(28816..29082)
                     /gene="pmrD"
                     /locus_tag="ACHUZQ_001008"
     CDS             complement(28816..29082)
                     /gene="pmrD"
                     /locus_tag="ACHUZQ_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1024765.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(29192..30547)
                     /gene="menE"
                     /locus_tag="ACHUZQ_001009"
     CDS             complement(29192..30547)
                     /gene="menE"
                     /locus_tag="ACHUZQ_001009"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1024766.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(30544..31506)
                     /gene="menC"
                     /locus_tag="ACHUZQ_001010"
     CDS             complement(30544..31506)
                     /gene="menC"
                     /locus_tag="ACHUZQ_001010"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1024767.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(31506..32363)
                     /gene="menB"
                     /locus_tag="ACHUZQ_001011"
     CDS             complement(31506..32363)
                     /gene="menB"
                     /locus_tag="ACHUZQ_001011"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1024768.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(32378..33136)
                     /gene="menH"
                     /locus_tag="ACHUZQ_001012"
     CDS             complement(32378..33136)
                     /gene="menH"
                     /locus_tag="ACHUZQ_001012"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1024769.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(33133..34803)
                     /gene="menD"
                     /locus_tag="ACHUZQ_001013"
     CDS             complement(33133..34803)
                     /gene="menD"
                     /locus_tag="ACHUZQ_001013"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1024770.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(34892..36187)
                     /gene="menF"
                     /locus_tag="ACHUZQ_001014"
     CDS             complement(34892..36187)
                     /gene="menF"
                     /locus_tag="ACHUZQ_001014"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1024771.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(36266..36571)
                     /gene="elaB"
                     /locus_tag="ACHUZQ_001015"
     CDS             complement(36266..36571)
                     /gene="elaB"
                     /locus_tag="ACHUZQ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1024772.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(36626..37087)
                     /locus_tag="ACHUZQ_001016"
     CDS             complement(36626..37087)
                     /locus_tag="ACHUZQ_001016"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1024773.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            37152..38069
                     /gene="rbn"
                     /locus_tag="ACHUZQ_001017"
     CDS             37152..38069
                     /gene="rbn"
                     /locus_tag="ACHUZQ_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1024774.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         9300 a        10257 c         9467 g         9045 t
ORIGIN      
        1 gcaatggcta ttttgttatt atttgatgaa taatatcagt gcgtcataat tcaagttaat
       61 aaccttaagg gataccagtt atatttaaac taaattatag taatgaataa ttttcttata
      121 atataaggta aattaacaaa atggcttcgc atttaacaat aaccgaatag aaaacaacca
      181 tttcgacatc aacaatcgct tacattcgct tatatattga ccacaactga tacatcagat
      241 tatgtgatga ctcgtgctta gatcaatttt tgcaatcatt aacaaaaaga ttaataaacc
      301 atctatatca atttatccaa cctattctgc cgttcaagaa atcgccgaac agttattttt
      361 aacaaatttt tctcttccca ttgactttcc cggacacctt gtctgaccta aggtgcgcga
      421 aagccacttt ttccttcctg agttatccac aaagttatgc acttgcaaga gggtcatttt
      481 cacactatct tgcagtgaat cccaaacata ccccctatat atagtgttct aagcagcttc
      541 ccttactaca ggtagtctgc atgaaactat tgcggaaaga attccaaaaa caggtacgac
      601 atacatgaat cagaatctgc tggtgacaaa gcgcgacggt agcacagagc gcatcaatct
      661 cgacaaaatc catcgcgttc tggattgggc ggcagaagga ctgcataacg tttcgatttc
      721 ccaggtcgag ctgcgctccc acattcagtt ttatgacggt atcaagacct ctgacatcca
      781 cgaaaccatt atcaaggctg ccgcagacct gatctcccgt gatgcgccgg attatcagta
      841 tctcgccgcg cgcctggcga tcttccacct gcgtaaaaaa gcctacggcc agtttgagcc
      901 gcctgcgctg tacgaccacg tggtgaaaat ggtcgagatg ggcaaatacg ataatcatct
      961 gctggaagac tacacggaag aagagttcaa gcagatggac acgtttatcg atcacgaccg
     1021 tgatatgacc ttctcttatg ctgccgttaa gcagctggaa ggcaaatatc tggtacagaa
     1081 ccgcgtgacc ggcgaaatct atgagagcgc ccagttcctt tatattctgg ttgccgcgtg
     1141 cctgttctcg aactacccgc gtgaaacgcg cctgcaatat gtgaagcgtt tttacgacgc
     1201 ggtttccaca tttaaaattt cgctgccgac gccaatcatg tccggcgtgc gtaccccgac
     1261 tcgtcagttc agctcctgcg tactgatcga gtgcggtgac agcctggatt ccatcaacgc
     1321 cacctccagc gcgattgtta aatacgtttc ccagcgtgcc gggatcggca tcaacgccgg
     1381 gcgtattcgt gcgctgggta gcccgattcg cggtggtgaa gcgttccata ccggctgcat
     1441 tccgttctac aaacatttcc agacagcggt gaaatcctgc tctcagggcg gtgtgcgcgg
     1501 cggtgcggca acgctgttct acccgatgtg gcatctggaa gtggaaagcc tgctggtgtt
     1561 gaaaaacaac cgtggtgtgg aaggcaaccg cgtgcgtcat atggactacg gggtacaaat
     1621 caacaaactg atgtataccc gtctgctgaa aggtgaagat atcaccctgt tcagcccgtc
     1681 cgacgtaccg gggctgtacg acgcgttctt cgccgatcag gaagagtttg aacgtctgta
     1741 taccaaatat gagaaagacg acagcatccg caagcagcgt gtgaaagccg ttgagctgtt
     1801 ctcgctgatg atgcaggaac gtgcgtctac cggtcgtatc tatattcaga acgttgacca
     1861 ctgcaatacc catagcccgt ttgatccggc catcgcgcca gtgcgtcagt ctaacctgtg
     1921 cctggagata gccctgccga ccaaaccgct gaacgacgtc aacgacgaga acggtgaaat
     1981 cgcgctgtgt acgctgtctg ctttcaacct gggcgcaatt aataacctgg atgaactgga
     2041 agagctggca attctggcgg ttcgtgcact tgacgcgctg ctggattatc aggattaccc
     2101 gatcccggcc gccaaacgtg gagcgatggg tcgtcgtacg ctgggtattg gtgtgatcaa
     2161 cttcgcttac tacctggcga agcacggtaa acgctactcc gacggcagcg ccaacaacct
     2221 gacgcataaa accttcgaag ccattcagta ttacctgctg aaagcctcta atgagctggc
     2281 gaaagagcaa ggcgcgtgcc cgtggtttaa cgaaaccact tacgcgaaag ggatcctgcc
     2341 gatcgatacc tataagaaag atctggatac catcgctaat gagccgctgc attacgactg
     2401 ggaagcattg cgtgaatcaa tcaaaacgca cggcctgcgt aactctacgc tttctgccct
     2461 gatgccgtcc gagacctctt cgcagatctc taacgccact aacggtattg aaccgccgcg
     2521 cggttacgtc agcatcaaag cgtcgaaaga cggtattttg cgccaggtgg tgccggacta
     2581 cgagcacctg cacgacgcct atgagctgct gtgggaaatg ccgggtaacg atggctacct
     2641 gcaactggta ggtatcatgc agaaatttat cgatcagtcg atctctgcta acaccaacta
     2701 cgatccgtca cgtttcccgt caggaaaagt gccgatgcag cagttgctga aagacctgct
     2761 caccgcctac aaattcgggg tcaaaacgct gtattatcag aacacccgtg acggcgctga
     2821 agacgcgcag gacgatctgg taccgtcaat ccaggacgat ggctgcgaaa gcggcgcatg
     2881 taagatctga tattgagatg ccggatgcgg cgtaaacgcc ttatccggcc tacggctcgg
     2941 tttgtaggcc tgataagacg cgccagcgtc gcatcaggct ccgggtgccg gatgcagcgt
     3001 gaacgcctta tccggcctac ggctcggatt tgtaggcctg ataagacgcg ccagcgtcgc
     3061 atcaggcaca ggatgcggcg taaaatgcct tatccggcat taaactccca acaggacaca
     3121 ctcatggcat ataccacctt ttcacagacg aaaaatgatc agctcaaaga accgatgttc
     3181 tttggtcagc cggtcaacgt ggctcgctac gatcagcaaa aatatgacat cttcgaaaag
     3241 ctgatcgaaa agcagctctc tttcttctgg cgtccggaag aagttgacgt ctcccgcgac
     3301 cgtatagatt accaggcgct gccggagcac gaaaaacaca tctttatcag caacctgaaa
     3361 tatcagacgc tgctggattc cattcagggt cgtagcccga acgtggcgct attgccgctt
     3421 atttctattc cggaactgga aacctgggtc gaaacctggg cgttctcaga aacgattcat
     3481 tcccgttcct atactcatat cattcgtaat atcgttaacg atccgtctgt tgtgtttgac
     3541 gatatcgtca ccaacgagca gatccagaaa cgtgcggaag ggatctccag ctattacgat
     3601 gaactgatcg aaatgaccag ctactggcac cttctgggcg aaggcaccca caccgttaac
     3661 ggtaaaaccg taaccgttag cctgcgcgag ctgaagaaaa aactgtatct ctgcctgatg
     3721 agcgttaacg cgctggaagc gatccgcttc tacgtcagct ttgcttgttc ctttgccttt
     3781 gcagaacgcg aactgatgga aggtaacgcc aagattattc gcctgattgc ccgcgatgaa
     3841 gccctgcacc tgaccggtac tcagcatatg ctgaatctgc tgcgcagcgg cgcggatgat
     3901 ccggagatgg cggaaattgc cgaagaatgt aagcaggagt gctatgacct gtttgtccag
     3961 gcggcacagc aagagaaaga ctgggcggat tatctgttcc gtgacggttc gatgattggt
     4021 ctgaataaag atattctctg ccagtacgtt gaatacatca ccaatatccg catgcaggca
     4081 gtcggtttgg atctgccgtt ccagacgcgc tccaacccga tcccgtggat caacacctgg
     4141 ctggtgtctg ataacgtgca ggttgctccg caggaagtgg aagtcagttc ttatctggtc
     4201 gggcagattg acgcggaagt ggacaccgac gatttgagta acttccagct ctgatggccc
     4261 gcgttaccct gcgcatcact ggcacacaac tgctgtgcca ggatgaacac ccttcccttc
     4321 tggcggcgct ggaatcccac aatgttgcgg ttgagtacca gtgccgcgaa ggttactgcg
     4381 gctcctgtcg cacccgactg gtcgcaggtc aggtggactg gattgccgaa ccgttagcct
     4441 ttattcagcc gggggaaatt ttgccctgtt gttgccgggc aaaaggcgat attgaaatcg
     4501 agatgtgaat tggttgtagt gccagataca acgcttatac gtcttatctg gcctacaacg
     4561 attacatggc gtagtaatac gctttcacct gctcccagtc cgctttgggg attggctcca
     4621 gatattttcc agctggcgga agtcatgatt aatcgcttta ttgcgacgca agcggcgacg
     4681 acttttctcc aggtcaagaa aaccagcttc tgctttacct tctgttttca catagatatg
     4741 gcgaacataa caacagccat gctgacgatt aacgctatgc attttcttaa acgccagcgc
     4801 cactgctttc aacatggctt gccgtacttc gtcagaatag ggcgatactg catgccgggc
     4861 ataccagtca gcaatgctga tgaaccccgc catatcttca gtcaccagca acgcgcgcca
     4921 ttcaccctca attttcaccg cttcaccaaa aacgatcttc ggcacgatga caccggcccg
     4981 ttccagttct ttaattaccg caacctcacg gacaatcgtt ggtcggccga acggataacg
     5041 tacggaatga aacagatgat gcgtcatgcg ctttacatac agctttttgc cgttgcgctc
     5101 gacgcattgc accccgctca taccattacg gcgatagtta ggctcttcaa cccagtcgcc
     5161 ctctgttgcc caccagtgat taaattcgtc gtactttgct gaaactgcca taccctatcg
     5221 cctgtcattt ttattaacga caatgactat aggtggttac ctgaggaaaa tcttaatgaa
     5281 acgtgtcgta ttaatgaatt tcttttgccg ctactagtgt gacaccgctt ttgcccttca
     5341 tttgcagcgc atagattcca ccagccacga ccactaatag tgcgcccagc agataaaatg
     5401 tgaatttaat caggtggata cagcgatgaa ttttattcga cagggattag gcatcgcctt
     5461 attaccagag ttaacgctga aaaccattgc aggtgaattg tgttccgttc ctcacgaacc
     5521 aaccttctat cgacagattt cgttgttggc taaagaaaag ccggtagaag gcagtccact
     5581 gtttttacta caaatgtgca tggaacaatt agtggcgatt ggaaaaattt gatatgagac
     5641 aggatggcgc atgaacgcca tcctgcagta gcattactct ttattaagga attttactgc
     5701 cttatcaggg aaatcagtaa acagcccatt tacacccgct ttgttataca gagcatcata
     5761 taactgattc acatcagtag tgtattcagg cagtttatcc gaccgcacgg tataaggatg
     5821 cactaccagt ttattctgct gagcatcttg caccatgcca gtgagtttga tattacccgg
     5881 ctgcgatgtt tcctcaatca acatatggta atccggcccg ataccgtcgg catattccgc
     5941 gacttgcttc atggcacctg gcttaaacat ccagtcatag ctgtaattaa cccagcttcc
     6001 gtccggctgt ttctgctgcg tttcattcca gtcggtatag gcaatcagct gtaccagatt
     6061 gagatccatg cccattttag gttccagctc attcttaata cgcttcagct catcagcatc
     6121 aaaacattgc aaataaactt tatcgtcctt accggtgtaa ccatatttct tcagcacttc
     6181 cagcgttttt gccgcaatat ccttcccttc ctgatgatgg aaccacggcg ctttgatttc
     6241 tggatagata ccgatatttt tcccggtaga gtgatttaac ccctgaacaa attcaatctc
     6301 ttcttcaaag gtgtgccccc ggaagtcgga cttacccatt gggaaacgcc ccggataagt
     6361 ctgtactttt ttaccgtttt caatatcgaa accttcggta aatttcagcg acttaatttc
     6421 atccagcgtg aaatctatcg cgtagtaacg accgtctttg cgcgcccgat ccgggaaacg
     6481 atcggcaaca tcagtaacac gatcgagata atggtcatgc agaacaacca gatggtcgtc
     6541 tttggtcatc accaaatcct gttccagata atccgctccc tgcgcatacg ccatcgcttt
     6601 tgctggcagc gtatgctccg gcaaatatcc actggcaccg cgatgggcga tgactatttt
     6661 ttcgttgctg tccgccgcca ttgcactgct tcccatgact atagtgctca tcatgatcgc
     6721 catgctaagg tttttcagct tcaatttcat gccattagcc tccgttgcgt ttttgcagta
     6781 attgttcatg gcgacgtttt tcgccaatca tcacaacaat caacaagata accgccagaa
     6841 tgctgccgcc aatcattacc ataaagccgc catcccagcc gaagaagtcc acggtgtagc
     6901 caacaatcgc gctcgccgcc accgaaccgc ccaggtaacc aaacagcccg gtaaagcccg
     6961 ctgccgtacc tgccgctttt ttcggtgcca gttccagcgc atgcagaccg atcagcatca
     7021 caggaccgta gatcaggaag ccgataacaa tcatacaaat catatcgacg gttgggttac
     7081 ctgccgggtt catccagtaa acgatagtcg cgatggtcac cagtgtcata aagaaaacgc
     7141 cggttgcccc acggttgcca cggaagactt tatccgacat ccagccgcac agcagagtgc
     7201 ccggaatacc tgcatattca taaaggaagt aggcccagga ggatttatcc agcgcgaaat
     7261 gcttaacctc tttcagataa gtcggtgacc agtcgaggat gccgtaacgc agcagataaa
     7321 cgaacacgtt ggcgatggcg atataccaca gcagtttgtt cggcagtacg tactgcatga
     7381 agatttgctt cgccgtcagc tcctgttccg ctttttcgtt atagtcgtcc ggataatcat
     7441 ttttgtactc ttcgatcggc ggcaagccac aggattgcgg ggtatcgcgc atcatcgcaa
     7501 aggcgaataa tgccaccaga atggcgcaga aagcaggcat atagagcgcc gcatgccagt
     7561 cattgaacca ggccatcccc agcaggaaca gcagcggcgg aataccacca ccgacgttgt
     7621 gcgcacagtt ccacactgac acaatgccgc cacgttcttt ctgcgaccac cagtgcacca
     7681 tagtacgacc acacggcggc caccccatcc cctggaacca accgcagagg aacaacagta
     7741 caaacatcac cgcaatgctc gacgtcgccc atggcacaaa gcccataaac aacatcactg
     7801 ccgccgccag aatcaaacct gcgggcagga aaacgcgcgg attcgagcga tccgataccg
     7861 aacccatgat gaatttcgaa aatccataag caatcgagat ccccgaaagg gcaaaaccta
     7921 aatcaccgcg tgagaatccc tgctcaacca gataaggcat agcaagcgca aagttcttac
     7981 gaaccaaata gtaagccgca tagccaaaga atatccccag gaaaatttgc cagcgcaatc
     8041 gacgataagt cggatcgatc tccgcggcag gtaagcgcgc tttgtgtggc gctggtttaa
     8101 aaatactcaa cattgatagc ctccgtggcc cgtggtctta tttatgatta acagcctgat
     8161 tcagtgagag aacctgccgt ttcttgagtt gccgcgatgt taagaaaaca ttcataaatt
     8221 aaatgtgaat tgccgcacac attattaaat aagatttaca aaatgttcaa aatgacgcat
     8281 gaaatcacgt ttcactttcg aatcatgagc gaatatgcgc gaaatcaaac aattcatgtt
     8341 tttactatgg ctaaatggta aaaaacgaac ttcagaggga taacaatgaa aactcgcgac
     8401 tcgcaatcaa gtgacgtgat tatcattggc ggcggcgcaa cgggagccgg gattgcccgc
     8461 gactgtgccc tgcgcgggct gcgcgtgatt ttggttgagc gccacgacat cgcaaccggt
     8521 gccaccgggc gtaaccacgg cctgctacac agcggtgcgc gctatgcggt aaccgatgcg
     8581 gaatcggccc gcgaatgcat tagtgaaaac cagatcctga aacgcattgc acgtcactgc
     8641 gttgaaccaa ccaacggcct gtttattacc ctgccggaag atgacctctc cttccaggcc
     8701 acttttattc gcgcctgcga agaagcaggg atcagcgcag aagctataga cccgcagcaa
     8761 gcgcgcatta tcgaacctgc cgttaacccg gcattgattg gcgcggtgaa agttccggat
     8821 ggcaccgttg atccatttcg tctgaccgca gcaaacatgc tggatgccaa agaacacggt
     8881 gccgttatcc ttaccgctca tgaagtcacg ggactgattc gtgaaggcgc gacggtgtgc
     8941 ggtgttcgtg tacgtaacca tctcaccggc gaaactcagg cccttcatgc gcctgtcgtg
     9001 gttaatgccg ccgggatctg ggggcaacac attgccgaat atgccgatct gcgcattcgc
     9061 atgttcccgg cgaaaggatc gctgctgatc atggatcacc gcattaacca gcatgtgatc
     9121 aaccgctgcc gtaaaccttc cgacgccgat attctggtgc ctggcgatac catttcgctg
     9181 attggcacca cctctttacg tattgattac aacgagattg acgataatcg agtgacggca
     9241 gaagaggttg atattctgct gcgtgaaggg gaaaaactgg cccccgtgat ggcgaaaacg
     9301 cgcattttgc gggcctattc tggcgtgcgc ccgctggttg ccagcgatga cgacccgagc
     9361 ggacgtaacg tcagccgtgg catcgtgctg ctcgaccatg ctgaacgcga tggtctggac
     9421 ggatttatca ccatcaccgg tggcaaactg atgacctatc ggctgatggc tgaatgggct
     9481 accgacgccg tgtgccgcaa actgggcaac acgcgcccct gtacgaccgc cgatctggca
     9541 cttcctggtt cacaggaacc cgctgaagtt accttgcgta aagtcatctc cctgcctgcc
     9601 ccgctgcgcg gttctgcggt ttatcgtcat ggcgatcgca cgcctgcctg gctgagcgaa
     9661 ggtcgtctgc accgtagcct ggtatgtgag tgcgaagcgg taacagcggg tgaagtgcag
     9721 tacgcggtag aaaatttaaa cgttaatagc ctgctggatt tacgccgtcg tacccgtgtg
     9781 gggatgggta cctgccaggg cgaactctgc gcctgccgcg ctgccggact gctgcaacgt
     9841 tttaacgtca cgacgtccgc gcaatctatc gagcaacttt ccaccttcct taacgaacgc
     9901 tggaaaggcg tgcaacccat cgcctgggga gatgcactgc gcgaaagcga atttacccgc
     9961 tgggtttatc agggactgtg tggtctggag aaggagcaga aagatgcgct ttgatactgt
    10021 cattatgggc ggcggcctcg ccggattact ctgtggcctg caactgcaaa aacacggcct
    10081 gcgctgtgcc attgtcacgc gtggtcaaag cgcgctgcat ttctcatccg gttcgctgga
    10141 tttgctgagc catctgcctg atggtcaacc ggtgacagac attcacagtg gactggaatc
    10201 tttgcgtcag caggcaccag cccatcctta cagtcttctc gaaccacaac gcgtgctcga
    10261 tctcgcttgc caggcgcagg cattgatcgc tgaaagcggt gcgcaattgc agggcagcgt
    10321 ggaactggcc catcagcgcg ttaccccgct cggcactttg cgtgcaacct ggttaagttc
    10381 gccagaagtt cccgtctggc cgctacccgc gaagaagata tgtgtagtgg ggataagcgg
    10441 gctgatggat ttccaggcgc atcttgcggc ggcttcgttg cgcgaactgg atctttcggt
    10501 tgaaaccgca gaaatagagc tgccggaact ggatgtcctg cgcaataacg ccaccgaatt
    10561 tcgcgcggtg aatatcgccc gtttccttga taatgaagaa aactggccgc tgttacttga
    10621 tgcgcttatt cctgtcgcca atacctgcga aatgatcctg atgcccgcct gcttcggcct
    10681 ggccgatgac aaactgtggc gttggttgaa tgaaaaacta ccttgttcac tgatgctttt
    10741 gccaacgctg ccgccttccg tgctgggcat tcgtctgcaa aaccagttac agcgccagtt
    10801 tgtgcgccag ggtggcgtgt ggatgccggg cgatgaagtg aaaaaagtga cctgtaaaaa
    10861 tggcgtagtg aatgaaatct ggacccgcaa tcacgccgat attccgctac gtccacgttt
    10921 cgcggttctc gccagcggca gtttctttag tggcggactg gtagcggaac gtaacggcat
    10981 tcgcgagccg attctcggcc ttgatgtgct acaaaccgcc acgcggggtg aatggtataa
    11041 gggcgatttt tttgcgccgc aaccgtggca gcagttcggt gtaaccactg atgagacgct
    11101 acgcccgtcg caggcagggc aaaccattga gaacctgttt gccatcggtt cggtgctggg
    11161 cggatttgat cccatcgccc agggatgcgg cggcggtgtt tgtgccgtca gtgctttaca
    11221 tgccgctcaa cagattgccc aacgcgcagg aggccaacaa tgaacgacac cagcttcgaa
    11281 aactgcatta agtgcaccgt ctgcaccacc gcctgcccgg tgagccgggt gaatcccggt
    11341 tatccagggc caaaacaagc cgggccggat ggcgagcgtc tgcgtttgaa agatggcgca
    11401 ctgtatgacg aggcgctgaa atattgcatc aactgcaaac gttgtgaagt cgcctgcccg
    11461 tccgatgtga agattggcga tattatccag cgcgcgcggg caaaatatga caccacgcgc
    11521 ccgtcgctgc gtaattttgt gttgagtcat accgacctga tgggtagcgt ttccacgccg
    11581 ttcgcaccaa tcgtcaacac cgctacctcg ctgaaaccgg tgcggcagct gcttgatgcg
    11641 gcgttaaaaa tcgatcatcg tcgcacgcta ccgaaatact ccttcggcac gttccgtcgc
    11701 tggtatcgca gcatagcggc tcagcaagca caatataaag accaggtcgc tttctttcac
    11761 ggctgcttcg ttaactacaa ccatccgcag ttaggtaaag atttaattaa agtgctcaac
    11821 gcaatgggta tcggtgtaca actgctcagc aaagaaaaat gctgcggcgt accgctaatc
    11881 gccaacggct ttaccgataa agcacgcaaa caggcaatta cgaatgtaga gtcgatccgc
    11941 gaagctgtgg gagtaaaagg cattccggtg attgccacct cctcaacctg tacatttgcc
    12001 ctgcgcgacg aatacccgga agtgctgaat gtcgacaaca aaggcttgcg cgatcatatc
    12061 gaactggcaa cccgctggct gtggcgcaag ctggacgaag gcaaaacgtt accgctgaaa
    12121 ccgctgccgc tgaaagtggt ttatcacact ccgtgccata tggaaaaaat gggctggacg
    12181 ctctacaccc tggagctgtt gcgtaaaatc ccggggcttg agttaacggt gctggattcc
    12241 cagtgctgcg gtattgcggg tacttacggt ttcaaaaaag agaactaccc cacctcacaa
    12301 gccatcggcg caccactgtt ccgccagata gaagaaagcg gcgcagatct ggtggtcacc
    12361 gactgcgaaa cctgtaaatg gcagattgag atgtccacaa gtcttcgctg cgaacatccg
    12421 attacgctac tggcccaggc gctggcttaa actcctttct gatgcccggt aagcatgtgg
    12481 ttaccgggca tttttgcgta cacgattccg tgcccaatgt atgcgttgca acgcagtgaa
    12541 aattcctctg aaaacgtctc gcaaaggctg aaactggcag atgtcaaagg cctgggataa
    12601 ccgtaatgtc gcgtcatcat aaatatcagg tgacggacaa ccatgaccga atcaacaacc
    12661 tcctccccgc atgatgcggt atttaaaacc tttatgttca cacccgaaac cgcacgggat
    12721 tttctcgaaa tacatttgcc agaaccactg cgcaagcttt gcaacctgca aaccttacgc
    12781 ctggaaccca ctagttttat tgaaaaaagt ttacgcgctt actactcgga tgttttgtgg
    12841 tccgtggaaa ccagcgacgg tgacggctat atctactgcg tgattgaaca tcaaagctct
    12901 gcagaaaaga atatggcttt tcggctaatg cgctatgcca ctgccgccat gcagcgtcac
    12961 ctggataaag gctatgacag agttccgctg gtggtgccat tgctgtttta tcatggcgaa
    13021 acatcgccct acccgtactc acttaactgg ctggatgagt ttgacgatcc gcaacttgcc
    13081 cggcagttgt acaccgaagc ttttccgttg gtggatatca ccatcgtacc tgacgatgag
    13141 atcatgcaac atcggcgtat agctctgctt gaactgattc aaaagcatat tcgcgaccgc
    13201 gatttaatcg gtatggtcga caggatcacc acgcttttgg ttaaaggctt cactaatgac
    13261 agccagctac aaacactgtt taattatctg ctgcaatgcg gcgatacctc ccgtttcacc
    13321 cgttttattg aggagatttc cgaacgttca ccactacaaa aggagagatt gatgactatt
    13381 gctgagcggc tacggcagga aggacaccaa attggctggc aggaaggtat gcatgaacaa
    13441 gccattaaaa ttgctttgcg catgctggaa cagggcattg atcgtgacca ggtgctcgcg
    13501 gccacccagc taagcgaagc cgatctggca gcgaataacc actaattaac acaggcccac
    13561 agccgatccc catgggcctt tgatatcaat aactaccttt tatgcgtggc ccatttttgc
    13621 ctgatttaaa catcgccagt cgttgatcca gggcatcgct gtagagcatc gtgtcaacgc
    13681 caacagcgac aaagttcgct ccccacgcca ggcattgctg cgccatatca ggagccacag
    13741 ccagaaaacc agccgcttta cccgcagcac ggatccgccg aatgctggtt tcaataattc
    13801 gctgcacttc cgggtgcccg gcgttatccg ggtagcccaa cgacgcagaa agatccgctg
    13861 gtccaataaa cacgccatca atcccttcga cgtcgaggat ctcgtccagg ttatccagtg
    13921 ccgttttact ttccacctgc actaacagac aaagcgaatc gttgacttgc gccatgtaat
    13981 tctcaatgcg tccccagcgc gcagcccgtg ccacactggc cccaacacca cgctcaccgt
    14041 agggaggata gcgcgtggca gacgccacct gacgagcctg atcggcagta tcgaccatcg
    14101 ggatcaatag agtttgcgcg ccaatatcca gaacttgttt aatcagcgat ttactgcctt
    14161 ccaccggacg gatcacgggt tggctggcat agggcgctac cgcctgtagc tggtgataaa
    14221 gatcctgaat ggtgtttggc gcgtgctccc cgtcaatcag caaccagtca taaccggaag
    14281 tagcggcaat ttctgccata taagaagtag tcgagcttaa ccacagacca atttgcactt
    14341 cgcccttgcg taaacgttct ttaaagggat tggttaataa tgcgttcatc gtgttcctta
    14401 acctatttaa tgatgtgcca cgtcggtctg cgcaacagta cgatttacac gcagagtgaa
    14461 aataatcagc gaaccgatga ccgccaccgc tgccagtgtc agtaatcctg ccgcatcgct
    14521 ggcaaataac gtttctgctt tcacgcgcag gatcggggca ataaacccgc ccactgcacc
    14581 aaacaggttt acaaaaccaa tccccgcagc cagcgccgta ccggaaagca gttgtgtcgg
    14641 catcgtccag aacaccggct gcacggcaat aaacccgata gctgcaacac acagcgctac
    14701 gatcgccatt actggagaaa gcagcccgga cagaccaata ccaatgcccg ccgccagtaa
    14761 tgtcagcgct gcgacattac gccgttcgcc ggttttatcg gaatagcgcg gaataagcca
    14821 ggtcccaaac aaggccgcaa cccacggaat ggcggtgacc accgacgctg taaagcccac
    14881 ttttgttccc agcaatgccg caacctgggt cggcaggaag aaaattaatc cgtaaaccgc
    14941 cacctgaatg gtcaggtaga taatcgctag ttgccagact cggccattac gcagcgcatc
    15001 gctcagccga gaagtcactt tctgttgttc ttcacttgcc agttgattga taagcaacgt
    15061 tttttcttgt ttactcagaa aacgtgcctg ctccggtgtg tcatcaagcc aaaagaatgt
    15121 gaatacccca gcaccgactg ccaacaatcc ttcaatcaca aacatccaga accagccggg
    15181 atgccccata aatccatgca tctccaacag cgcgccggaa agcggtgatc ccagtgttaa
    15241 cgccagcggt gcgcccatat agaacagccc cataatgctg gcgcgattac gctgcggaaa
    15301 ccattgcgag gtgagataaa tcataccagg gaaaaatccg gcctccgcag caccaagcag
    15361 agtgcgaact atcagaaatt tcgcttcagt atcggcccat gccatggctg ccgaaagaaa
    15421 tccccacagc agtgttgtcg taccaatcca ggttctggcc cccagtttgc gcatcaaaag
    15481 attcgccgga acacccagaa acgcatatac cacaaagaaa atgcctgctc ccagcgcata
    15541 agcttcatta ctcaacccgg tatcaatctg gtaggtctgt ttggcaaaac cgatattcga
    15601 acggtcgaga aacgccagca catacagcgc caacataaac ggaattaaac gcacacggtt
    15661 tttcttcacc acggcgtcaa gcaaagcggt gctcataaaa agctccttag aatatgtggg
    15721 cgttaccgtt gtcacagcaa cgcccggagt gattagtggc tgtaggggcg tttcaaattg
    15781 cagtcacgat tgagttcgac gccaaaaccg ggtttatcaa gcactgattt atgaatacga
    15841 ccattcaccg gaaccggctc attgagcaga atcgggtcaa actgcggacg catcgttgaa
    15901 caatccgggc tggtcatcag gaattcgctg aatggcgtat tggtgaaggt gatcaccgca
    15961 tggtgagagt aaacagacga accgtgcggc accaccagtt gccccctgga tttggcgatt
    16021 gcggcaattt ccaccagcgt ggttaaacca ccgcaccagc caacatccgg ctgcataatg
    16081 tcgataccgg tttctgaaag cgtacgaaaa gattgcagtg tgccgtggtg ctcaccgctg
    16141 gtgaccatca ttccgactgg cgcgttgcgt ttcagttcgc gataaccttc atactgctgt
    16201 ggcggcaggc actcttcgat ccatttcagg ttatagggcg cgcaagcgtg ggccagtttg
    16261 gtggcatagt tcacgtcctg actcatccag cagtcgagca ttaaccagaa atcctcaccg
    16321 catttttcac gcatatccgc gaccataacg gcatctttgc ggatccccgc atcgccatca
    16381 tgtggccccc agtgcgtcgg cattttgcca ccgataaagc ccatctcttt tgccagatcc
    16441 ggacgcgcgc ctgtggcgta gaactgaatc tcatcacgaa ctgcgccgcc taaaagttta
    16501 taaaccggaa gcccgaccac tttgccgaac agatcccaca gagccagatc gacacaggaa
    16561 atcgtgttca tcaccaggcc accagagccg gagtaataca gggtggcatt gagcatttga
    16621 tcatggatca gtttgatatc actgacacat ttaccctcaa tgaaacggtt aagatgtttt
    16681 tcgacaataa aacagcccat ttcaccggct gtcgaaacag cgaatccggt ctggccgttt
    16741 tctgcttcga cttcaacaat caacgtgcca agaacgttaa tgccaaatga ctggcgtgac
    16801 tgctcgtaat cgcggtattt actcatcggg gtggcaatat gatcgtcaat ccagtgattc
    16861 gccccctggt cgtgataatc accgccgcca gcaccttttt ctgctgtcgc accgccagta
    16921 aaccaggcgc gaacctgttt gatttttggt agggtcatga tgttctccat tgttatgagg
    16981 cctgtaagtc aaagggactt ttccatccca acagacgtga aatatccctg gcgcaggcaa
    17041 tggccttgcc cgccagataa tcacggtatt cttcattgat ttgtaagcgg gtaccgacca
    17101 ccgagatcgc agcggtaagc tcgttattgg cgttaaacac cggcgcagcg acacaacgga
    17161 catcggggta atcttcgccg ttgtcatagc tccagccctg acggcgaata cgcgccagtt
    17221 cttcgtgaag ttgctgtgga tgagtaatcg ttgtgggtgt cgcctgctcc cagaccagcc
    17281 cttcgataat actttgctgt accgctgcag gttgccaggc aagcaggcat ttacctattc
    17341 cggagcgata aagcgaaagg ctttttcctt catgggaacg cacgctgata gtggctgatg
    17401 actccacttt taaaatgtaa taagcgctgc cgttatcaat aatccccagg tgacataaca
    17461 gcccggtggt atccatcagt tgcgtcaaac gcggtcgagc cagttcccgg agatccatct
    17521 tgcttaacgc atgaccagaa agctccacca gcctggtcca caaacaaaaa ttttcctggt
    17581 gatcaaggct aagaaaacgc tgacgcctga gttcattaag cagcaaatag gccgtgcttt
    17641 tagggattcc caatgtgtca atgatcgtcg cagcactaca ggggccaatg cgggcaatca
    17701 aattgagaat atcaatagcc cgtgtcaggg cgggaacttt gcttgattcc aacatactgg
    17761 actccagtct tagatactgg aatcagtctc gttgctcagg gggagtaaaa ttgtgaagcg
    17821 catcaaatcc cgctcctcgt ccagcggact ggagaaatta cgcacaaatt tgtgaatcag
    17881 tgcacggatt ttttcttatt gagtacaaca ggttggacaa gttttgccgg agggggaatg
    17941 tgccggtgca gtaaaccggc acagagtggc ggaatcagac agataaggtc atggactcaa
    18001 caacctcaat ccagccatgc tcactggcga tatcctggcc gtttaaccaa cggcgcagca
    18061 tattcagtgc catcattgcg cacacttcct gacggatagc caggctgtag cgtgtagtgc
    18121 tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc tagcgcaaag ttgagatgct
    18181 cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa atggttggcc cgccgttctg
    18241 taatccagtg cgcagtttgc gccagggttt cttcctgtga aggaaccact tcacacgcca
    18301 gcaatggagc acctgcgcga gaaagttgca aagccaataa accaccggtg aattgctcgc
    18361 tcaacgtcag gctgaactgg cggttttgca attcgcgact gatctgcgcg ggcagtcctt
    18421 cggtgccttc gaaaatcacg ctctgtccgg caacacgttt aacgtccagc cacagttttt
    18481 ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag ttcgatgatc ggcattgagg
    18541 agcgatagcc cattgttaca cccggcggca gttgtagagt gtccaggctt tgtgccagat
    18601 cgctttccga acgaccaaag gtagttaaac gcagacaaac cggcgactgt ggtaaagaaa
    18661 aacgctcgcg caggcgcggc aggatttcgt gctcgaccat caccttaaat tctgacggta
    18721 cgccgggagt aaagaacatc aggcaacgat taagccgcac ggcaaaacca caggcggtgc
    18781 ctaccgggtt attgataaat tcagcactgg caggcagctc cgcttgttta cggttgctcg
    18841 gtgccattac tcgtccacgt tcgtggaaat agcgttccat ctctttgagc caggcttcat
    18901 gcagcaccag gccttcacct tttgctgtcg cagcggcgag tgcacttaaa tcatcactgg
    18961 tcggtcccag cccgccgtta acgatcagca catcggcgtg ctgactacgt tcgcgaagaa
    19021 tggtgactaa gtcatcaagg ttatccccca ccgtattgcg gcgagataat ggcaacccct
    19081 gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc aacgatttgc ccgtgtaaca
    19141 cttcatcccc ggtggataac atttccactt ttaacatggt tatctcctgc ttttgttatc
    19201 gaatcactat aacgcaaagg gagataaggc gtagtaagaa actgaatggc gcgacagaat
    19261 gccgcgccgg aagggatcag aaactggcgt taaccccaac atacgggccg tcagccacgg
    19321 cgttgtcgcg gttaccgtct ttacccgaca gattcaggta gcgataaccc gcctcaatac
    19381 tgactggatg cataatggtg taacgcgcgc cagcattcgc ttcctcataa cttttaatac
    19441 cgctggagag tgaatccgga gagtagtaat actcgccaaa caaacggaag ctgttgccaa
    19501 ttttccactg caaaccacct cctaccgctg cggcataacc ttcatcgccg taattcgggt
    19561 tggtgtaaac gccttttccg ccaacggtcg ccattaacgg cccgagaggc agattcaacc
    19621 ccagccccac gccagcgacg tcaccgtcgt cgtcgttatg tgtccagtta ccgcttaacg
    19681 ccaggcccgt cgattcagtc ccaaaaccga caccaatatt ggtgtattct ttacccgcct
    19741 gaccgctgat gctcattgca ttgaccgatg cagaaaccaa cagcattccg gccagacctg
    19801 ctagcgcaat ttttttcatt atcccctttc ccacaagcaa ttaatcaaaa ttcccaaagc
    19861 tgcgggattg tactgcctgg cacagaggat tcaatgagcg aaaaaaggga tctagtcact
    19921 tattgtaact aaatacttct taaataatta aacgccgaaa aaataaacac actttaccga
    19981 tacacacaaa aataataata agtgcagtta tcatcgcaaa agagattact gtcactttca
    20041 catatttctg agttaccgta ttctcatggt ttctttttat ttataaggaa tacccctgtg
    20101 cgacaacgga ctattgtatg ccctttgatt caaaatgatg gtgcttattt gctgtgcaaa
    20161 atggccgacg atcgcggcgt tttccccggt caatgggcgc tttcgggtgg cggcgtggag
    20221 tctggcgaac gaattgaaga ggcactacgc cgcgaaattc gcgaagaact gggagaacag
    20281 ctgcttttga cagaaatcac gccgtggacc ttcagcgatg atattcgcac caagacgtat
    20341 gcagatggtc gcaaggaaga gatttatatg atttacctga tttttgactg cgtttctgcc
    20401 aaccgagaag tgaaaataaa cgaagagttt caggactacg cgtgggtaaa acctgaagat
    20461 ctggtgcatt atgatttaaa tgttgccacc cgaaaaacgt tacgtttgaa aggtcttctt
    20521 taacaatgat gacagccatc actggctgtc agattgaaaa tttagtggtt aacgaattcc
    20581 ccatccagat aaactttgcc tttttccaca tgcatgacta aaccatccag ataatcaggt
    20641 ttaaatgtcg catcacgctt atttttagca atatatgtca ggcaatgatt atgggtgaaa
    20701 ataacgatat ttttatcagg cgctttgctt tgtaagtcct taattgcact ataaatctca
    20761 ttaccgcact gaagaagtcg tttatctacc gtcagttttt tacccgcgga aaaccaggtc
    20821 gccgactgaa tggtccggac ggtattactg gaataaagat cgaaatcagg gatgtcagcg
    20881 ctaaaagcgt tgcccagttc acgggcatcc tgggtacctt taaccgtaat acctgtttta
    20941 tctgacaagc attgattggt tgaacgatcg caacgttcag catggcgaaa caaaacgaca
    21001 accgggtgct gctgtgccag tctggccaat gttttattgt cgatacgtgg caaaccattg
    21061 ctactccagg cggcatgagt acccagtccg gcaattgcag cgagcgccag taaaatgatg
    21121 aaatattttt ttgacttcaa tgatgagcgg caaaaagcta acacgtaaac tccacctata
    21181 gacaagcgca accagacaat taccgtgaaa ttgagcgaca tttctggcga taattagcag
    21241 ttggtgtaat attaaaaatc ctacgatgtc ggcaaaatgc ctcaaaattt tgccaaatgc
    21301 aaagcctaaa taagaaaaaa tataaaaatt tcaatattta cgtctaatat tagtttctta
    21361 aggttaagtt aatattctat ccttaaaatt tcgctccaaa tggcaaaata tacacaacac
    21421 tctttatagc aaatataagt ggacaggtat tcaatggcgg aaggaaaagc aatgtcagaa
    21481 tttttgcctt tttcgcgacc agcaatgggc gtggaggaac tcgctgcagt taaagaggtt
    21541 ctcgaatccg gttggatcac aaccggtccg aagaatcagg cgcttgagca agccttttgc
    21601 cagttgacgg gaaatcagca tgccatcgcg gtcagttcag ccaccgccgg aatgcatatc
    21661 acgctaatgg cgttggaaat tggcaagggc gatgaagtga ttacgccttc cctgacctgg
    21721 gtttcaaccc tcaatatgat ttccttgttg ggtgcaacgc cggtaatggt ggatgtcgac
    21781 cgcgatacgc tgatggtcac gcctgaagcg atcgagtcag ccattacgcc acgcactaaa
    21841 gccatcattc cggtgcatta tgccggtgcg ccagcagata ttgacgccat tcgcgccatt
    21901 ggcgaacgtt acggcatcgc agttatcgaa gatgctgccc atgccgtcgg tacgtattac
    21961 aaagggcgac atattggcgc aaaaggtacc gctatttttt catttcatgc cattaaaaat
    22021 attacctgtg cggaaggtgg cctgattgta actgataatg aaaaccttgc ccgccagcta
    22081 cggatgctga aatttcacgg tctgggtgtc gatgcctatg acagacaaac ctggggccgt
    22141 gcaccgcagg ctgaagtctt aacaccgggc tataagtaca atctgaccga tattaacgcc
    22201 gcgattgccc tgacacagtt agcaaaatta gagcacctca acacccgtcg gcgcgaaatt
    22261 gcccagcaat atcagcaagc actggcagct ctcccctttc agccattaag ccttcccgcc
    22321 tggccgcacg ttcacgcctg gcatctgttt attattcgtg tcgatgaaca acgttgtggt
    22381 atcagtcgcg atgcgttgat ggaagcgtta aaagaaagag gcattggtac cgggttacat
    22441 ttccgcgccg ctcacacaca aaaatattat cgcgagcgtt ttcccacgct gtcgttaccg
    22501 aataccgaat ggaatagcga acgcatctgt tctttgccgc tgttcccgga tatgactacc
    22561 gccgatgccg accgtgtcat cacagccctt cagcaactcg caggacaata agccatgttt
    22621 gaaatccacc ctgttaagaa agtctcggtg gttattcccg tttataacga gcaggaaagc
    22681 ttaccggaat taatcaggcg caccaccaca gcctgtgaat cgttggggaa agagtatgag
    22741 atcctgctga ttgatgacgg cagtagcgat aattccgcgc atatgctggt cgaagcctca
    22801 caagcggaga acagccatat tgtgtctatt ttgcttaacc gcaattacgg gcaacattca
    22861 gcgattatgg cgggattcag tcacgttact ggcgacttaa ttattaccct tgatgccgat
    22921 ctccagaatc cgccagaaga aatcccccgc ctggtggcaa aagccgatga aggttacgac
    22981 gtggtaggga ctgtacgcca gaaccgccag gacagctggt ttcgtaaaac agcttcgaag
    23041 atgattaaca ggcttattca gcgcaccacc ggcaaagcga tgggtgacta cggttgtatg
    23101 ctgcgcgcct atcgccgtca tattgtcgat gcgatgttgc actgccatga acgtagcacc
    23161 tttatcccga ttctggcgaa tatcttcgcc cgccgtgcca ttgaaattcc agtacatcat
    23221 gccgagcgtg agtttggtga atccaaatac agttttatgc gcctgattaa tttgatgtac
    23281 gacctggtga cctgccttac cacaacgccg ctacgtatgc tgagtctgct cggcagcatt
    23341 attgcgattg gaggttttag cattgcggtg ctgctggtga ttttacgcct gaccttcgga
    23401 ccacaatggg cggcagaagg cgtctttatg ctatttgccg tgctgtttac ttttattggc
    23461 gctcagttta tcggcatggg attgctcggt gaatatatcg gcaggatcta caccgatgtc
    23521 cgcgcccgcc cccgctattt tgttcagcaa gttatccgtc catccagcaa ggaaaatgaa
    23581 taatgaaaac cgtcgttttt gcctaccacg atatgggatg cctcggtatt gaagctctgc
    23641 tggctgccgg ttacgaaatt agcgccattt ttacccatac cgataatccc gctgaaaaag
    23701 ccttttatgg ttcggtggct cgtctggcgg cggaaagagg cattccagtt tatgcgccgg
    23761 ataacgttaa tcatccgctg tgggtggaac gcattgccca actctcacca gaggtgattt
    23821 tctcttttta ttatcgccat cttatttgcg acgagatttt gcagctcgct cccgcaggtg
    23881 catttaatct gcacggttcg ctgttaccaa aatatcgtgg tcgcgcgccg ctgaactggg
    23941 tgctggtgaa cggtgaaacg gaaacgggcg ttacattgca ccgaatggta aaacgtgccg
    24001 atgctggggc cattgtggcg caactgcgcg ttgccattgc gccagacgat atcgctatta
    24061 cgctgcatca taaattgtgc catgccgcgc gccagctact ggaacagaca ttacccgcca
    24121 ttaaacacgg taatattctg gaaattgccc agcgcgaaaa cgaagccacc tgttttggtc
    24181 acagaacgcc agatgacagt ttccttgaat ggcataaacc ggcatccgta ctgcacaaca
    24241 tggtacgtgc cgttgccgat ccgtggccgg gtgccttcag ctatgttggc aatcagaaat
    24301 tcaccgtctg gtcgtcgcgt gttcatcctc atgccagcaa agcacagccg gggagcgtga
    24361 tttctgttgc gccactgctg attgcctgtg gcgatggcgc gctggaaatc gtcaccggac
    24421 aggcgggcga cggcattact atgcagggct cgcaattagc gcagatgctg ggcctggtgc
    24481 aaggttcacg cttgaatagc cagcctgcct gcaccgcccg acgccgtacc cgggtactca
    24541 tcctcggggt gaatggcttt attggcaacc atctgacaga acgcctgctg cgcgaagatc
    24601 attatgaagt ttacggtctg gatattggca gcgatgcgat aagccgtttt ctgaatcatc
    24661 cgcattttca ctttgtcgaa ggcgatatca gtattcattc cgaatggatt gagtatcacg
    24721 tcaaaaaatg tgatgtcgtc ttgccgctgg tggcgatagc cacgccgatt gaatataccc
    24781 gcaacccgct gcgcgtattt gaactcgatt ttgaagagaa tctgcgcatt atccgctact
    24841 gcgttaagta ccgtaagcga atcatcttcc cgtcaacttc agaagtttat gggatgtgta
    24901 gcgataaata cttcgatgag gaccattcta atttaatcgt cggcccggtg aataaaccac
    24961 gctggattta ttcggtgtca aaacaattac ttgatcgggt gatctgggcc tatggcgaaa
    25021 aagagggttt acagttcacc ctcttccgcc cgtttaactg gatgggacca cgactggata
    25081 accttaatgc agcgcgaatt ggcagctccc gcgctattac gcaactcatt ctcaatctgg
    25141 tagaaggttc accaattaag ctaattgatg gcggaaaaca aaaacgctgc tttactgata
    25201 tccgggacgg tatcgaggcg ttgtaccgca ttatcgaaaa tgcgggaaat cgctgcgacg
    25261 gcgaaattat caacattggc aatcctgaga acgaagcgag cattgaggaa ctgggcgaga
    25321 tgctgctggc gagcttcgaa aaacatccgc tgcgccatca cttcccaccg tttgcgggct
    25381 ttcgtgttgt cgaaagtagc agctactacg gcaaaggata tcaggacgta gagcatcgta
    25441 aaccgagcat ccgcaatgcc cgccgctgcc tggactggga accgaaaatt gatatgcagg
    25501 aaaccatcga cgaaacgctg gatttcttcc tgcgcaccgt tgatcttacg gataaaccat
    25561 catgaccaaa gtcggcttac gcattgatgt cgataccttt cgtggcaccc gtgaaggcgt
    25621 gccacgtctg ctggaaatct tgagtaagca taatattcag gccagcattt ttttcagcgt
    25681 cggcccggac aatatgggcc gccatctctg gcgactggtg aagccacagt ttttgtggaa
    25741 gatgctgcgc tcaaacgcgg catcgcttta tggctgggat attttactgg caggtacggc
    25801 ctggccaggt aaagagattg gtcatgccaa tgccgatatc attcgtgaag cggctaaaca
    25861 tcacgaagtc ggcctgcacg cctgggatca ccatgcctgg caagcccgta gcggtaactg
    25921 ggatcggcaa acaatgatcg acgatattgc acgcggtctt cgcactctgg aagagattat
    25981 cggtcaaccg gtaacctgtt ctgccgctgc gggctggcgt gccgaccaga aggtgatcga
    26041 agcaaaagaa gcgttccatt tgcgctacaa cagcgattgt cgtggggcca tgccgttccg
    26101 tccactgctc gaatcaggaa accctggcac tgcgcaaatt ccggtgacct tacccacctg
    26161 ggatgaagtg attggtcggg atgtgaaagc agaagatttt aacggttggt tactcaaccg
    26221 catcctgcga gataaaggca cgccggttta taccattcat gcagaagttg aaggctgcgc
    26281 ttatcagcat aattttgtgg atctcctcaa acgcgcagct caggaaggcg tgacattttg
    26341 ccctttaagc gaactgttat cagagacgtt gccgctcgga caagttgttc gcggaaatat
    26401 tgccggacgt gaaggctggc tgggttgcca acaaattgcg ggtagtcgct gatgaaatcg
    26461 gtacgttacc ttatcggcct cttcgcgttt attgcctgct attacctgtt accgatcagc
    26521 acgcgtctgc tctggcaacc cgatgaaacg cgttatgcgg aaatcagtcg agaaatgctg
    26581 gcatccggcg actggattgt gccccatctg ttagggctac gttatttcga aaaacccatt
    26641 gccggatact ggattaacag cattgggcaa tggctatttg gcgcgaataa ctttggtgtg
    26701 cgggcaggcg ttatctttgc gaccctgtta actgccgcgc tggtgacctg gtttactctg
    26761 cgcttatggc gcgataaacg tctggctcta ctcgccacag taatttatct ctcattgttt
    26821 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg
    26881 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    26941 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    27001 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    27061 ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct cccctgggga
    27121 ctggcgatag cgcagcggga gcctgacttc tggcactatt ttttctgggt tgagcatatt
    27181 caacgctttg cactggatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    27241 atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta cacaggctgg
    27301 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    27361 tttttctccg tcgctaaagg taaattgccc acctatattc tttcctgctt tgcatctctg
    27421 gcaatgctga tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    27481 attaatggct ggattaacat cgcttttggc ttcactggca ttattgccac atttgtggtc
    27541 tccccgtggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    27601 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    27661 gaaaagacct ggtcttttgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    27721 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    27781 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    27841 gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    27901 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    27961 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    28021 gatatcaaca gtctcgccat tccgcccgca gatgccatcg atcgtcagga gcgtctggtg
    28081 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    28141 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac aaacggcgca
    28201 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    28261 ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta agtctgaatt
    28321 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    28381 ggtgtggggt ggcgttcatt attggcggca ttgtgatcct cgggagtacg gtgtaatggg
    28441 cctgatgtgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    28501 tgcggcgagt catctgccgc cgatgacgca cttatgggat tttatcgcgg ctctgttagc
    28561 ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    28621 ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    28681 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    28741 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    28801 cacgacaaaa caacgttact gagttttccc tgccacttta cactgccgtt cccactcatc
    28861 ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac gattaataca
    28921 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    28981 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    29041 atcttgttta ttgcagcacg attttttgac cagccattcc attgcattat cctgtttgct
    29101 aagagttttc acatcaattt acagattaac agataaacct attatcaacg gcaatggaat
    29161 ggcaaaattg cgaagtgaaa ctaatgattt cttaatcctg gcgttgcacc cactctttta
    29221 acgcctgacg tgaaatttta ataccgccgt ttttcagctc cggcggtaaa gttagccagc
    29281 gcaccggttg ttgaaaacga gccagcttat ctttcaccca ttcgccaaga tcaacggttt
    29341 gctggtcata ctccaccacc gctaccggtc gatgaccaaa ctccttgtcg gcaacgggga
    29401 cgataaacac ctgcaaaacc gcaggatgtg cagcaattac gcgctcgact tcttccggct
    29461 gaataccctc tccgccactg aagaacagat tgtctaaacg tccgacaatg gtcagcttgc
    29521 cattatgcat ctcaccgcga tcgcgcgtag cgtaccagcc ttcgtcatta accagtgaaa
    29581 ccagttgccc gttacgccag taaccttctg ccatactggc agcccgcagc cacacttcat
    29641 tattaacgat tttcacttcc cgacccggca gcggcgaacc aacgtctgcc aggccgtcgg
    29701 cttctttcgc acacaccgtg gaggcaaact cggtcagacc atagccgcaa aagcaacgaa
    29761 tcccctgctc gcgcgcctgt tccgtcaact cgaccgggat agccgcgccg ccaagtaaca
    29821 ccgctttcag ggaaacggaa ctgcggttaa ccagcaaacg ccagagttgt gttggcacca
    29881 gtgaagcgtg agtacagcct gccagcattt gctccaatgg ctgtttatca cgcaccgtca
    29941 tccgcgcacc agcgtataac cagcgccaca taattccctg accggagacg tgaaacagcg
    30001 gtaaagagag caaccagtca tcgtgatcgc caaacggaat cagcgataac acaccttgcg
    30061 cactggcaag atgggcctga taagtatgga cagcggcttt cggcaaaccg gtagaacccg
    30121 aggtcaacgt cattgagcac agacgcgtcg gctgccacgt agcggcatgt gcgccttcaa
    30181 cccgctgaat gtgcagcgac gttaatgccg gaaacgtgtt ttccccatcc ggcaccagcg
    30241 caaattgcag cgtcagattg ggcagcaatt cttcaagcaa cggttgcggc agctgagggt
    30301 tcacgggcaa cacccgcgcc ccgcattgca gtaacgccag ccaggcgagc agcgtttgcg
    30361 gcgtattcca cgcccgcaac atcacgccgc tgccctcaac cacaccctgc gccgcaaagc
    30421 ccgtagctaa ctcatcgacg cgagcacaaa gctcgcgcca gttgagttgc tcgtcattaa
    30481 gacgtaaggc gatggcttct ccccgcactt gccgccagtg acgccacggc cagtcagaga
    30541 agatcataac aactgctcca gtacatcagc gtccactaac ggcagtgggc tccccggcca
    30601 gcgacgaacc tgctgcgcct gcatcagatc cagcgtgtcc agtcccggaa tggtgtctgg
    30661 cgttaaccag gcagcaatcc gcgccagttg cgttagacct aagctcgact caatggaaga
    30721 actgatcacc gccgtcagcc ccagcgcatg cgccgcctgt acctgctcgc gtactttttc
    30781 cagactgccc gtgagcgtgg gtttgataac taccgcgcgc acgccctctt cagccacaaa
    30841 ggcaaaatcc ggctcgcgca ggctttcatc ccaggcaatg gcaatgccgg tttcacgggc
    30901 aaacgctcgc gaatcatcgc gggttttgca cggctcttcg agaaacgcga tgcggtggcg
    30961 ataatccggg ttaacatatt tggcaaactg ctgacctttc agcggtgtcc aggcgcgatt
    31021 tgcgtcaaga cgcaaatgca gatccggaat tgcctccagc aacagattaa ccaccatgcc
    31081 gtcgcgcacg gcttcgtaca atcccacttt gaccttcgcc actttctcgc ctggcatatc
    31141 agcaagtttg aggatcagat cgtcaggatc gccattacac agtggtgccg cacggtaatt
    31201 ggccgcctgc gggagcgtat ctgccagctc cgccaatgca cagcttacgc caaaggcaac
    31261 ggaaggcatc tgcggtagct cgccatcgcc tgccagccag ttatttaccc aagcaagcag
    31321 cacaatttgc gcctcttccc aggtttcctg actgaagccc ggcagtgggg agatctcccc
    31381 ccacccttcg cgctcgcctt cacgcaggca aacatacagc ccgtcgcggg tttttaaccg
    31441 cctgtcgcgc agaaccatcc ccgcgtccat ggggatctgc cagcggtata cctgcgcgct
    31501 acgcattacg gattccgttt aaatttgctg aaatcaggct gacgtttctg gttgaaagcg
    31561 ttgcgacctt cctgaccttc ttccgtcatg tagaacagca tggtggcgtt gcccgccagc
    31621 tcctgcagac ccgcctgacc gtcacagtcg gcgttcagcg cagctttcag gcagcgcagc
    31681 gccatcgggc tgttttgcag catttcgcgg caccaacgga cggtttcttt ttccagatcc
    31741 gccagcggta ccacggtgtt gacaaggccc atatccagcg cctgttttgc atcgtactga
    31801 cggcacagga accagatttc acgcgctttt ttctgtccga cgatgcgagc catgtaggaa
    31861 gcgccccagc cgccgtcgaa ggaaccgact ttcgggccag tctgaccgaa gatggcatta
    31921 tctgccgcga tagtcaggtc gcacatcatg tgcagaacgt gaccaccgcc gatggagtag
    31981 ccagccacca tcgcgacaac cggtttcgga caggtacgga tctgacgctg gaagtccagc
    32041 acgttcaggt gatgtacgcc ggaatcatct ttatagccgc cgtaatcacc acgcactttc
    32101 tggtcaccac cggagcagaa cgctttatcg cctgcgccag tcagaatgat cacgccgacg
    32161 ttgtcgtcat aacgcgcatc tgccagtgcc tggatcatct cttttaccgt cagaggacgg
    32221 aaggcattgc gcacctgcgg acgattaatg gtgatttttg cgataccgtc ggtggatttt
    32281 tcataacgaa tgtcctcaaa accttcggag cagtcgtgcc attcaaccgg tgcgtaaagc
    32341 attgcttcat caggataaat catattgtgt cctttagtca gaaacgcaag atctgcgcca
    32401 gacttgcgat aacgccagcg ggattttccc gatgcgcgtt atgtccggcg cgaggaatga
    32461 catggcagtc ggcagccagt tccgccgcca aggcgcggaa tttgctgtca cgttcaccac
    32521 ataaataata aaacgcaaat gtgcgggcgc taaggttagc acgtaaatca ggctggacgg
    32581 cgagagaagt cgcctccagc atggcggcaa gggttgcgcc attattgtta ctgcgcagcg
    32641 ccaccagctc ccggcgttga tcgtcattga gcgaggcaaa aacaggctgt tgataccagt
    32701 cggcaaatac cgccgttaac ggttctgtgc gaaaacgctg cgcccattgg cgatcggaac
    32761 gctgacgttc cgcgcgttgt tcagcatttt gcaacccagg atgcccgcct tcgacaacaa
    32821 ccccacaaag ccccggcatc ccctggcaag ccgccatcat cgccacccgt cccccaagcg
    32881 agtaccccac caaccagaag ttaaggatgt tgtaactaac cagggtttta cgcagtaagt
    32941 cggtgacatc atcaaatcca tcgacgctaa tcgccgccga accaccgtga cctgggagat
    33001 aaacatacaa ccgtgagtag tcggcaaacg cctcgcccac ttcttgccat tcgtggcaat
    33061 cgccggaaaa accgtgcaaa aacaccagcc agggtaaacc tggtttgccg tgttttgcct
    33121 gcgcgtgcag gatcataaat ggcttacctg cgccagaagt tgctggagcg tttgcgcacc
    33181 atcggtgtcg ttaaccacca tttcaattac cgtggtggtt ggcgtgcgcc aggcatcggc
    33241 aagtgccgtt tcaagttcct gccagttttg cggacgatga tatttcagct cgaacatcgc
    33301 ggcggcgtgc tcaaaatgga cgttttgcgg catcagatag aaacgctcgc gctcgctttt
    33361 cggcgttggc agcagcgaga aaatttgccc gccgttgttg ttcaccacaa ttaatatcag
    33421 cggcgcggaa acctgacgca ataacgccag cgcgttgaga tcgtaaagtg cggagagatc
    33481 gcccacaatc gccagcgtcg gtttgccgct tgcccgctga acgccggcgg cagtcgagag
    33541 cagcccgtcg ataccgctgg ccccacggtt gctgtacacc gggtaacctg ccggaagttg
    33601 cgaaagcgca tcaatcagac gtaccaccag gctgttaccg acaaacaatt gcccctgttc
    33661 aggcagatag tcgctgatgc gatgcgccag ttgcgcttcg ccaaacgcat cgcgacgggc
    33721 aataaccgcc tgcattgcct gttccgccag gcgcgggatt tcaacgcacc agggctggcg
    33781 tttttctgcc ggatgcagct ccagccagtc ggcaatattg gcaattaagc ggcgtccacg
    33841 atggtgcgcc ggatcaagcc gcccttcgat gtcatccaca atccagtact cttcgggttc
    33901 acagcttgcc tgccattgca ggagccgttt gcccgtcagg ctgcttccca gttgcaccac
    33961 aatttgcgcc tgttgcagtt cgctggtcgc tttggcattg cctaaccaga gatcggcaca
    34021 cggcagcggc tgtccggttt gcgacagcac gtcgccaatc agcggccagc caagagtttg
    34081 cgcccacagt gcaacttttt tgccctcttc cgcactcatg cgcccggcaa ccaccacgcc
    34141 gcgtttttgt cgccagaaga accagtcgcg ctgtttttca ctttccagac gaggcgcttc
    34201 acgcagccac ggtttgtcgt cctgccacca gtcgcccagc cgctgttgcc agctaagccc
    34261 ggtgtcgtcc atttcgccat acagcggttc ggcaaacggg cagttgatat ggactccacc
    34321 agaatgaagc gtaccgagag catggtcgat ggtagaaacc agccaacgtg cggggatatc
    34381 ctgggtcggg cgcggcaatg aaatactgtg cgtggggtga gaggcgaaca ttcccggctg
    34441 acgaatcgcc tgattcgcgc cgcagtcaat cagctccggc gggcgatcgg cggttaacag
    34501 gatcagtttt tctccggtta acccagcttc aatcagtgcc ggatagagat ttgctaccgc
    34561 cgtaccggag gtcacaatca ccgccaccgg ctgcttgctg actttcgcca gccccagcgc
    34621 cagatgcccc aacccacgct catcgaaatg ggtgtggtga atgaatgcgg aattctccgc
    34681 cgccgctaac gttaacggtg tagaacgcga gccaggggcg atacagatgt gtctgacgcc
    34741 gtgacgcgtt aatgcttcca gaatgaccgc cgcccagcgt cggttaaatg cgcttactga
    34801 catgagtttg tccggtatca atattgcggc taagtataag gagctacaaa aatagattat
    34861 tgatatgaat cggtaatgat gcgactcatt actattccat ctgtaataaa gtacgcagcc
    34921 ctgccgcttt gttgtcgatt tcctgccact cttgctcggg gtcggaacca cggacaatgc
    34981 ccgcgccagc atataatcgc acgacattgc cgctaatttt tgctgagcgc agggaaacgc
    35041 agaattcgct ttgctgtaat gagagatagc ccgcagaacc ggcgtaccat tctcgggtga
    35101 acggttcgtg acgggcgata aactgtcgcg ccagatcgcg cggtaagcca gcaactgctg
    35161 ccgttggctg caactgatgt aaacagatca cattatccgc tttgttgagt gaagtccaga
    35221 tacagcggcg aagatgctgc actttacgca gacgcagtac ctgcggcggt aaaacatcca
    35281 gcgtctgggt atcggcctgt aaccgttggc agatatcttc caccaccagc atattctcgc
    35341 gctggttttt atcatccgcc atcagccact cacctaactg ctgcgcctgc ttatcatcag
    35401 gatgatttgc taccgttccc gccagcgctt cagtacgcag cgctttgtca cgccgccgcc
    35461 ataaccgttc cggtgaagag ccaagaaaag cattttcgcc atcaaaggcc atgtaaaaat
    35521 ggtagcaatt cagattcagt cgacgactgg cagccatcat cgccgccgcg ttgaccggac
    35581 ttgcgaaatg caggtcagtt gcccgagcga gcaccacttt gtcgagctca ccttcggcga
    35641 tggttttcgt tgccagttcg attaattgcg tccagcccgt tttgtccggc cagtgttgtt
    35701 ctcgcgtggt ggttaaatgt aacccaggca agggcttgat actcaccagt gtggcgataa
    35761 attctcttgc cttaatcgca tcgtgctgaa gggagctttc gctgaatagc gtcagccgca
    35821 gcgtggcttt accgccacag cgtcgccatt ccaggcgtgg taaaagtaaa ttgccctgcg
    35881 acgggtcaaa cgcattcagc ccccaaatgc gtaagtcggc gtgttccggg tgctggcgaa
    35941 gaaaacgttg tgcctggtcc aacgacgtaa aacgggtaat cgcgcccagg acggcagctt
    36001 cttcatcacc attacgttgt tgccagtaga attgcgggta tgtttgctga ctggccagcc
    36061 agctcaaggc atcaaaagcg tctttgagag ggaaaggaat atcgataacc cgaatgccgg
    36121 gtgtcgccgg aatctcttgc gacaaatggc gcagtagatt ttccagcgcc gtagtaagtg
    36181 attgcacgcg gacctctccc tgttaaaagc ctcacattat acggggtact acaaaaaaaa
    36241 tgcagtaccc cggtgtaggg aggttttaac ggcgtgccag caacagtcct agtaccagcc
    36301 caacggccgc gcccacacca attccttgcc agggtttttc gtggacgtag tcatcagcac
    36361 gataaactgc ctgcttcgcc cgatagtaat aactgtctga agcctggcta acccgttttt
    36421 tcacatcatc cagcgctttt tctgcacgcg ctttcagctc aacatatttc tgatcggcgg
    36481 gatcgccaga ggagcggagc acctcttcca gtgtttcact aagcagcgtc aggtcgtcat
    36541 cgatacgtgt atcaccaaac tgattagaca ttctcgttct ccatttgcgt aaaacctgtc
    36601 actaactata gacaagggtt gttgattacg cctgaattac ctcgcgtgcc atcccaatgt
    36661 gtgggatacc atcttcttca tagacctccg tcacagggat aaaaccgaaa ctctggtaga
    36721 agttttgcag atgcgcctgc gccccgaggt aaacaggctt atcaggccag tgatgcgtgc
    36781 aggtgtcgag ggttttactc atcagttgct gccccacttt ttcgccacgc aacgcttcac
    36841 tgacaatcac ccgaccaata acgaccggct caagatcatc atcacttttc agaatcctcg
    36901 catatgccac cagttcatca tttttccaac cgagaatatg gcgattatcc cccgtcaaat
    36961 catcaccatc gatatcctga taagggcaat tctgctcgac aacaaacacc gcgcaacgca
    37021 gttgtaataa ggcataaagc tgggaaaccg ataattcaga gtgatgcaga tcttgccatt
    37081 caatcataat gctctccttt tagatgtatg ccttatacta gccggaatat tttttgaaac
    37141 gtgatgaact catggaatta atttttttag gtacttcagc cggtgtgcca acccgcacgc
    37201 gcaatgtcac ggcaatattg cttaacctgc aacatccgac ccagagcgga ctttggttgt
    37261 ttgactgcgg tgaaggcacc cagcatcagc tactgcatac cgcctttaac cctgggaaac
    37321 tggacaagat ttttatcagt caccttcatg gcgatcatct ctttggttta cccggcttgc
    37381 tgtgcagtcg ttctatgtca ggcattatcc aacccttaac gatttatggt ccgcaaggta
    37441 tccgtgaatt tgtggaaacc gcgctgcgga ttagcggctc atggaccgat tatccgctgg
    37501 aaattgtcga aattggcgct ggcgaaattc tcgatgatgg cctgcgcaaa gtgaccgctt
    37561 atccgatgga acacccactg gaatgttatg gctatcgtat tgaagaacat gataaaccgg
    37621 gtgcattaaa tgcccaggca ttaaaagctg ctggcgtgcc gcctggccca ctgtttcagg
    37681 aattaaaagc gggcaaaaca atcacgctgg aagatggaag gcagattaac ggcgcagatt
    37741 acttagctgc tccagtgcca ggtaaagcgc tcgctatttt cggcgatacc ggcccctgcg
    37801 atgccgcact tgacctggct aaaggtgtcg atgtcatggt gcacgaagcg acgctggata
    37861 taaccatgga agccaaagcc aatagtcgcg gccatagctc tacacgccag gctgcgacac
    37921 tagcccgtga ggctggagtc ggcaagctaa tcattaccca cgtcagctcg cgctatgatg
    37981 acaaaggttg tcaacacctg ttacgtgaat gcaggtcaat tttcccggcg actgaactgg
    38041 cgaatgattt caccgtgttt aacgtttaa
//
LOCUS       DAZPBM010000022        36886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPBM010000022 DAZPBM010000000
VERSION     DAZPBM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.022"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUZQ_001018"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUZQ_001018"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1024775.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHUZQ_001019"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHUZQ_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1024776.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUZQ_001020"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUZQ_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1024777.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUZQ_001021"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUZQ_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1024778.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUZQ_001022"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUZQ_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1024779.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHUZQ_001023"
     CDS             5280..5738
                     /locus_tag="ACHUZQ_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1024780.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHUZQ_001024"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHUZQ_001024"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1024781.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHUZQ_001025"
     tRNA            6790..6874
                     /locus_tag="ACHUZQ_001025"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHUZQ_001026"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHUZQ_001026"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUZQ_001027"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUZQ_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1024782.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUZQ_001028"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUZQ_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1024783.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUZQ_001029"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUZQ_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1024784.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHUZQ_001030"
     CDS             complement(10156..13899)
                     /locus_tag="ACHUZQ_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1024785.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUZQ_001031"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUZQ_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1024786.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="ACHUZQ_001032"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="ACHUZQ_001032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1024787.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="ACHUZQ_001033"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="ACHUZQ_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1024788.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUZQ_001034"
                     /gene_synonym="iatA"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUZQ_001034"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1024789.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUZQ_001035"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUZQ_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1024790.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUZQ_001036"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUZQ_001036"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1024791.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUZQ_001037"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUZQ_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1024792.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUZQ_001038"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUZQ_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1024793.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="ACHUZQ_001039"
     CDS             23294..23401
                     /locus_tag="ACHUZQ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1024794.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23829..23936
                     /locus_tag="ACHUZQ_001040"
     CDS             23829..23936
                     /locus_tag="ACHUZQ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1024795.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24085..24939)
                     /gene="kdsA"
                     /locus_tag="ACHUZQ_001041"
     CDS             complement(24085..24939)
                     /gene="kdsA"
                     /locus_tag="ACHUZQ_001041"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1024796.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24975..25784)
                     /gene="sirB1"
                     /locus_tag="ACHUZQ_001042"
     CDS             complement(24975..25784)
                     /gene="sirB1"
                     /locus_tag="ACHUZQ_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR1024797.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25788..26180)
                     /gene="sirB2"
                     /locus_tag="ACHUZQ_001043"
     CDS             complement(25788..26180)
                     /gene="sirB2"
                     /locus_tag="ACHUZQ_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1024798.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26177..27010)
                     /gene="prmC"
                     /locus_tag="ACHUZQ_001044"
     CDS             complement(26177..27010)
                     /gene="prmC"
                     /locus_tag="ACHUZQ_001044"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1024799.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27010..28092)
                     /gene="prfA"
                     /locus_tag="ACHUZQ_001045"
     CDS             complement(27010..28092)
                     /gene="prfA"
                     /locus_tag="ACHUZQ_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1024800.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28134..29390)
                     /gene="hemA"
                     /locus_tag="ACHUZQ_001046"
     CDS             complement(28134..29390)
                     /gene="hemA"
                     /locus_tag="ACHUZQ_001046"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1024801.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29604..30227
                     /gene="lolB"
                     /locus_tag="ACHUZQ_001047"
     CDS             29604..30227
                     /gene="lolB"
                     /locus_tag="ACHUZQ_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1024802.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30227..31078
                     /gene="ispE"
                     /locus_tag="ACHUZQ_001048"
     CDS             30227..31078
                     /gene="ispE"
                     /locus_tag="ACHUZQ_001048"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1024803.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31229..32176
                     /gene="prs"
                     /locus_tag="ACHUZQ_001049"
     CDS             31229..32176
                     /gene="prs"
                     /locus_tag="ACHUZQ_001049"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1024804.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            32300..33979
                     /gene="dauA"
                     /locus_tag="ACHUZQ_001050"
     CDS             32300..33979
                     /gene="dauA"
                     /locus_tag="ACHUZQ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1024805.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34034..34312)
                     /gene="ychH"
                     /locus_tag="ACHUZQ_001051"
     CDS             complement(34034..34312)
                     /gene="ychH"
                     /locus_tag="ACHUZQ_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1024806.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34589..35173
                     /gene="pth"
                     /locus_tag="ACHUZQ_001052"
     CDS             34589..35173
                     /gene="pth"
                     /locus_tag="ACHUZQ_001052"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1024807.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            35290..36381
                     /gene="ychF"
                     /locus_tag="ACHUZQ_001053"
     CDS             35290..36381
                     /gene="ychF"
                     /locus_tag="ACHUZQ_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1024808.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9126 a         9601 c         9029 g         9130 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctcctccaca agtaaatatt ggcaacccga
    23521 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
    23581 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgaga cgtagtatcc
    23641 acatcaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggctttatc cctggtggca
    23701 ttggttgctg gaaagagaaa acccccgcac gttgagaggt ttaacctgac aacagaacgg
    23761 gggctaatct tgaaccagaa caattcaaga atagccgcaa aatgttgtca ttacaacaag
    23821 gcggctatat gacgctcgcg cagtttgcca tgattttctg gcacgacctg gcagcaccga
    23881 tcctggcggg aattattacc gcagcgattg tcggctggtg gcgtaaccgg aagtaaggtg
    23941 aaaacgggcg tcaggctgcc gtgatggcaa tatgcgcccg aaaccggccg caggggtaac
    24001 agcctgcggc cttttttgta tctggcaccc atgaaaaaag tcttaacgca gaacgctaag
    24061 actttatttt tcaagcaaaa aagattactt gctggtatcc agttcttcga aacctttcac
    24121 cagatcatca atcgctttca tctgcttgag gaacggttcc agtttagcca gcggcagcgc
    24181 ggatggacca tcacatttcg catgttccgg atccggatgc gcttcaataa acagccccgc
    24241 cagacctacc gccataccgg ctcgtgccag ctcagccacc tgagcacgac gaccaccgga
    24301 agctgcgcca aacggatcgc ggcattgcag tgcgtgggtc acgtcgaaaa tcaccggcga
    24361 gttaccagac actttcttca taatgctgaa gcccagcata tcgacaacca ggttgtcata
    24421 gccgaagtta gcaccgcgat cgcaaagaat cactttttcg ttgccgcctt ctttgaattt
    24481 atcaacgata ttacccatct gtcccgggct gacaaactgt ggtttcttga cgttaattac
    24541 cgcaccggtt ttcgccatgg cttcaaccag gtcagtctgg cgagcaagaa acgccggcaa
    24601 ctgaatcaca tccacgacat cagcaacggg ctgtgcctga cttggttcgt gaacgtcggt
    24661 gataattttc acgccaaaag tctgcttcaa ctcctggaag attttcatcc cttcttccag
    24721 gcccggtcca cgataagagt ggatggagga gcggttggct ttgtcaaaag aggctttgaa
    24781 cacgtaaggg atacccagtt tctgggtcac ggttacgtag tgctcgcaaa tgcgcatcgc
    24841 cagatcgcga gattccagca cgttcatacc accaaacagt acgaacgaca ggtcatttgc
    24901 tacgttgatg tcgccaatgc taaccacttt ttgtttcata ggatcgcctt aatcttgagt
    24961 aaaatgtcga ttaattaatg cagcacaata tgtttatgcg cgatgttatt tatttgcgca
    25021 cggatcattt cgctgatcgg gtcttccgga cactgttcaa cgaaataact taaatcgttc
    25081 aacgcaacgt gttcgcaatc cagttgcgca taaatcaacc cgcgatcgcg aatttcatag
    25141 ggatcttccg ggttgaattg taataaagct tcgctggtgc gtaacgccag ctccatctga
    25201 ttttcttcca tcaacgaggc tttgagtgta tccaacaatt tgcggattac ctcaatgtta
    25261 tcagcttcat caaggtcttc ataaaacagt tccgccgacg ggctgatatt tccctttaac
    25321 catacgtcca gcatatgttc gcttaacgat tcaccgttaa aaggattaat cagccaaatt
    25381 tcgccatccg gacattcaat gcgcaatatc agctgcgtag ggaaaatcac cggcagcagc
    25441 ggcaaatcga gacgattcgc gacccataat aaaaccgcac ctaatgatac cgcactgccc
    25501 tgtcgattct ttaacacctg gtccagccat aatgcatcgg aaagacgata aacgccgcgt
    25561 gaggctttaa atccccagtc gccgtaaaac agcgcgatca gtttttccag ctgctcttct
    25621 aaaggcagaa gctggctgat ttcttccttc gccagactaa cgagacgctc cagttcgtcg
    25681 taaacatctt gcgagggaaa atcgcggcgg attgcttcgc aagccaggat catgccttcg
    25741 cacaatggcg ctttattaaa ttcgaaatca gctaacgatc tcatgactta ccccagtaac
    25801 ggtacttttg tggtggcgag tttaatgatg atgtacagca ccaccaacgc cagcgggaag
    25861 gcaataatgc gcgcctgctg actgtgcata cgacgataat cgagtgcaat aaaacccaaa
    25921 acgatataaa tgataactcc aaacagcttt tcagtcagcc actgtgcctg cccggaaaat
    25981 ggcaggatgt gcgctttagc catcaacgca atgccgctta acaataacac cgtatcgaca
    26041 accggcggca caatgcgcgt ccagcgagca aatgcctgtg gatgcttctg ataacgtagc
    26101 cagaaacgta aggttaatag cccaacagaa agcgcgatac taataagatg aacactaagc
    26161 agtgtagaaa aacttgtcat tgataatagc ggccgagcgt tacgcgctcg ttatcaccat
    26221 agtcacggca ggtttcgacg tcatgatacc ccgcgaggat aaatgcttgt cgcaccgctt
    26281 cgccctgctg ccagccatgt tccagaagca gaaagccgcc ggataccagc gcgttacgcg
    26341 actgttcgat gatatgcacg atgtctgcca ttccactgtc tgccgcaacc agcgcagtga
    26401 gcggctcaaa gcggacatcg ccttgttgaa gatgtgggtc ctgctcgtca atatacggcg
    26461 gattgctgac aatcatcgca aactgctgcc cggctagcgc gctaaaccag tcgctttgca
    26521 gaatgtggat atttttgatc gccagatgct gggcattacg ttgtgccagg gagacagcat
    26581 caggcatacg atctacagcg ataatttcgc agtccgggcg ctcgctagcc agcgcaagcg
    26641 caatcgcccc ggtacccgtc ccgagatcga gaatacggca aggttgttca ggcaaccgcg
    26701 ccagtgcctg ctccaccaga cactccgtat ccgggcgcgg aattaaggtc gctggcgaaa
    26761 caaataacgg caacgaccag aattctcgca ccccggttaa atgagcaatg ggttcaccat
    26821 cgcgacgacg tgtcagtagc gcatcaagtt gctgacattg ttcgtcagtc agctgcgttt
    26881 caccaaaggc cagaataaaa gtccgccccc tgccagtgac atactccaat aagatttcag
    26941 cgtcacggcg tgggctttcg ctcgcctgaa gttggcttat tgcttcacgt aaccagtatt
    27001 gatattccat tattcctgct cggacaacgc cgccagttgg tcggcctgat gttcctggat
    27061 aatcggttca atcagcatat ccagcttacc ttccatcact tcatccagac ggtagagcgt
    27121 caggttgatg cggtgatcgg taacgcgccc ctgcgggaag ttgtaagtac ggttacggtc
    27181 gctgcgatcg ccactcccca gcaggttacg acgggtagac gcttcggcct gttgccgttt
    27241 tgccatttct gcagcgtgga tacgagcacc aagtacagaa agtgctttag ctttgttttt
    27301 atgttgtgaa cgttcgtcct gacattcaac aacaatcccg gtcggcaagt gagtaatacg
    27361 aattgccgaa tcggtggtgt taacgtgctg accacccgcc cctgacgagc ggaaagtatc
    27421 aatgcgtaaa tctgctgggt tgatgtccgg cagttctgcg tcaggcagtt ctggcattac
    27481 cgcaacggta caagcagaag tatgaatacg accctgcgat tccgtagcag gaacacgctg
    27541 cacgcgatga ccgccggatt caaatttcag acgaccatac acaccatcac cgctaatttt
    27601 ggcgatgatc tctttataac caccatgttc accctcgctg gcgctcatga tttctacccg
    27661 ccagcggcgg gcttcggcat aacggctgta catacggaac agatcgcccg cgaacagcgc
    27721 cgcttcgtcg ccgccggttc cggctcggac ttcgaggaag gcgttacgtt cgtcatcagg
    27781 atcttttggc agtaacagaa cctgtaattg ctgttccagt tgctcgcttt tttctttagc
    27841 ttcgcgcagt tcatcctgcg ccatctcacg catttcagga tcatcgagca tcatctgtgc
    27901 ggtttcgata tcttcctgaa cctgttgcca gtcggtaaaa cagcgcgaaa catcacttaa
    27961 ctgcgcatat tcgcgtgata atgcgcgaaa acgttcctgg tcggcgatag tttgcgcgtc
    28021 acccagcaac gcctgaactt cttcatggcg ttcatgcagg gcttccagtt tggcaacgat
    28081 agaaggcttc ataggcgtaa atgcaccctg taaaaaaaga aaatgatgta ctgctactcc
    28141 agcccgaggc tgtcgcgcag aatattcagg cgttcgttat ccccgtcacg ggcggcctgt
    28201 tgaagtgatt tcgttggcgc atggatcaag cggttagtca gtttccatgc cagatcctgc
    28261 ataatggctt gcgcgtcgcc gccctgctca agggccgcta acgctttggc ggttaactca
    28321 tcgcgaacgt gctctgcctg gctgcgatac tcgcgaatgg tttcgctggc gctttgtgct
    28381 cgcagccacg ccataaattc gctggtttcc tgagcgacaa tagtttccgc ctcaaccgct
    28441 gcggctttac gctgcgccag gttgtgcgaa atgatgcttt gcagatcgtc cacactataa
    28501 agataagcat tcgccagttt gccaacttcc ggctcaacat cgcgcggaac ggcaatatcc
    28561 accaacagca ttggttggtt acgacgactt tttaatgcgc gctccaccat gcctttcccg
    28621 ataatcggta acgggctggc ggtggaactg atgatgatat cggcttcgcg cagacgttcg
    28681 tcgatctcac tcagggcaat cacttccgcg ccaacttcat ctgccagaat ttgggcacgt
    28741 tcgcgagtgc ggttggcgat aatcatcttc tgtactttat gttcgcgcag atgacgcgct
    28801 accagctcga tggtttcgcc cgcgcctacc agcaacactg tgaccgtaga gagcgattca
    28861 aagatctgcc gcgccagcgt acaagccgca aaagcgacag acacagcact ggcaccgata
    28921 tctgtttcag tgcgaacgcg tttcgctaca gagaaagatt tctggaacat gcgttccagt
    28981 tcgctggcct tcatatgacc tttttgcgaa tcggcaaacg cttttttaac ctgaccgagg
    29041 atctgcggct ccccaagaac cagtgaatcc aggccgctgg caacacgcat taaatggcta
    29101 accgcgtcgt tatcctgatg ccagtagagg cttttacgca gatcttcttc attaagattg
    29161 tgataatcgc aaagccagcg gattaacgcc tcttgcaggt tatcctgctc ttcaacgcta
    29221 agataaagtt ccgtgcggtt gcacgtcgac agcaccacgc cgccctgcac catcggctgc
    29281 gcaagcaggc tgtcaagcgc ctgatcgagc ttatccggcg aaaacgatac acgttctcgc
    29341 agcgatacag gtgccgtttt atggttgata ccgagtgcta aaagggtcat gtctgcggga
    29401 aataatacca acgttgatat ggttagtctg cttgcatcat acaggatgcg taagatcaat
    29461 aaaagagagc gccccctttt ggagtaattg ccgaaagccg ttagattctg gcaattaaga
    29521 caacttgaac atagacgata gcggacggta acgctagcat taagggttat aactgcaacg
    29581 tatctcaagg acttgtcatc actatgcccc tgcccgattt tcgtcttatc cgcctgctac
    29641 cgctggctgc tcttgtgctc actgcctgtt ccgttaccac gcccaaaggt cctggcaaaa
    29701 gcccggattc gccacaatgg cgtcagcatc agcaagacgt gcgcaatctt aatcagtatc
    29761 agactcgcgg cgcgttcgct tatatttctg accaacaaaa agtgtacgcc cgctttttct
    29821 ggcagcaaac cggccaggat cgctaccgtc tgctgctcac taacccattg ggcagcacgg
    29881 aactggagct gaatgctcaa ccgggtaacg tgcagttagt cgacaataaa ggtcagcgtt
    29941 ataccgccga tgacgccgaa gagatgattg gcaaattgac cggaatgcca attccgctca
    30001 acagcttgcg ccaatggatt ttaggtttac cgggtgatgc aaccgactac aaactggacg
    30061 accagtaccg cctgagcgaa attacctaca gccagaatgg caaaaactgg aaggttgttt
    30121 atggtggtta tgacaccaaa acgcaacctg cgatgccagc caatatggaa ctcaccgacg
    30181 gtggtcaacg catcaagtta aaaatggata actggatagt gaaataatgc ggacacagtg
    30241 gccctctccg gcaaaactta atctgttttt atacattacc ggtcagcgtg cggatggtta
    30301 ccacacgctg caaacgctgt ttcagtttct tgattacggc gacaccatca gcattgagct
    30361 tcgtgacgat ggggatattc gtctgttaac gcccgttgaa ggcgtggaac atgaagataa
    30421 cctgatcgtt cgcgcagcgc ggttgttgat gaaaactgcg gcagacagcg ggcgtcttcc
    30481 gacgggaagc ggtgcgaata tcagcattga caagcgtttg ccgatgggcg gcggtctcgg
    30541 cggtggttca tccaatgccg cgacggtcct ggtggcatta aatcatctct ggcaatgcgg
    30601 gctaagcatg gatgagctgg cggaaatggg gctgacgctg ggcgcagatg ttcctgtctt
    30661 tgttcggggg catgccgcgt ttgccgaagg cgttggtgaa atactaacgc cggtggatcc
    30721 gccagagaag tggtatctgg tggcgcaccc tggtgtaagt attccgacac cagtgatttt
    30781 taaagatcct gaactcccgc gcaatacgcc aaaaaggtca atagaaacgt tgctaaaatg
    30841 tgaattcagc aatgattgcg aggttatcgc aagaaaacgt tttcgcgagg ttgatgcggt
    30901 gctttcctgg ctgttagaat acgccccgtc gcgcctgact gggacagggg cctgtgtctt
    30961 tgctgaattt gatacagagt ctgaagcccg ccaggtgcta gagcaagccc cggaatggct
    31021 caatggcttt gtggcgaaag gtgttaatct ttccccattg cacagagcca tgctttaagc
    31081 cgggcaagct gagtttcggt gacaacgtca ccttgttcca gacgttgcat cgcgctcttt
    31141 aatacaccgc ctggaaagga tcatgcctgg cccgcacagt tttcggcaga ttctttccac
    31201 caatggacgc atgcctgagg ttcttctcgt gcctgatatg aagctttttg ctggtaacgc
    31261 caccccggaa ctagcacaac gtattgccaa ccgcctgtac acttcactcg gcgacgccgc
    31321 tgtaggtcgc tttagcgacg gcgaagtcag tgtacaaatc aacgaaaatg tacgcggtgg
    31381 tgatattttc atcatccagt ccacttgtgc ccctactaac gacaacctga tggaattagt
    31441 cgttatggtt gatgccctgc gtcgtgcttc cgcaggtcgt atcaccgctg ttatccccta
    31501 ctttggctat gcgcgccagg accgtcgcgt ccgttccgct cgtgtaccaa tcactgcgaa
    31561 agtggttgca gacttcctct ccagcgtcgg tgttgaccgt gtgctgacag tggatctgca
    31621 cgctgaacag attcagggtt tcttcgacgt tccggttgat aacgtatttg gtagcccgat
    31681 cctgctggaa gacatgctgc agctgaatct ggataaccca attgtggttt ctccggacat
    31741 cggcggcgtt gtgcgtgccc gcgctatcgc taagctgctg aacgataccg atatggcaat
    31801 catcgacaaa cgtcgtccgc gtgcgaacgt ttcccaggtg atgcatatca tcggtgacgt
    31861 tgcaggtcgt gactgcgtac tggtcgatga tatgatcgac actggcggta cgctgtgtaa
    31921 agctgctgaa gctctgaaag aacgtggtgc taaacgtgta tttgcgtacg cgactcaccc
    31981 gatcttctct ggcaacgcgg cgaacaacct gcgtaactct gtaattgatg aagtcgttgt
    32041 ctgcgatacc attccgctga gcgatgaaat caaatcactg ccgaacgtgc gtactctgac
    32101 cctgtcaggt atgctggccg aagcgattcg tcgtatcagc aacgaagaat cgatctctgc
    32161 catgttcgaa cactaatcga acccgctcaa agacccgctg cggcgggttt ttttgtctgt
    32221 aatatccatt tgtatgacct atgcctcctt cacctgccat ttagttgaca gatgatgcgc
    32281 tcacggatga aacattattg tgaacaaaat attttcctca catgtgatgc ctttccgcgc
    32341 tctgatcgac gcttgctgga aagaaaaata tactgccgca cggtttaccc gtgacctgat
    32401 tgccgggata accgtcggga ttattgctat cccgctggcg atggcgttgg ctattggtag
    32461 tggtgtggca ccccagtacg gtttatatac cgcagctgtt gcggggattg tcattgctct
    32521 gacgggtggg tcacgcttta gcgtttccgg tccgactgcg gcatttgtgg taattctcta
    32581 tcccgtgtcg caacagtttg gactggcagg actgctggtt gcgaccttgc tgtcggggat
    32641 ctttttgatt ctgatgggtc tggcacgctt tggtcgcctg attgagtata ttccggtttc
    32701 cgtcacctta ggtttcacct cgggtatcgg gatcaccatc ggtaccatgc agattaaaga
    32761 ttttctcggt ctgcaaatgg cccatgtccc ggaacattat ctacaaaaag tcggcgcatt
    32821 atttatggcg ctgccgacca ttaatgtggg tgatgctgcc attggcattg tgacgctagg
    32881 tattcttgtt ttttggccgc gtctgggcat tcgtttaccc ggtcaccttc cggccttgct
    32941 ggctggttgc gcggtgatgg ggattgttaa cctgctcggc ggacatgttg ctaccatcgg
    33001 ttcgcaattc cactacgtcc tggccgatgg ttctcagggt aacggtattc cgcaactgct
    33061 gccgcaactg gtgctgccgt gggatctgcc taattcagaa ttcacgctaa cctgggattc
    33121 tattcgcaca ctgctgcctg cggcattctc aatggcaatg ctcggcgcaa tcgaatctct
    33181 gctctgcgcc gtggtactgg atggtatgac cgggacgaaa cacaaggcga acagcgaact
    33241 ggttggacag ggactgggga atattatcgc tccgttcttt ggtggtatta ccgctacagc
    33301 tgccatcgcg cgttctgccg ctaacgtccg tgccggggca acttccccta tctcggcggt
    33361 gatccactct attctggtta ttcttgccct gctggtactg gcaccgctgc tctcctggct
    33421 gccgctttcc gctatggcag ccctgctgtt gatggtggcg tggaacatga gtgaagcgca
    33481 taaagtggtc gacttgctgc gtcatgcacc gaaagatgac atcattgtca tgctgctgtg
    33541 catgtcgctg accgtgctgt ttgatatggt tattgccatc agcgtgggga tcgtgctggc
    33601 atcgctgctg tttatgcgtc gtatcgcacg tatgactcgc ctggcaccgg tagtcgtaga
    33661 tgttccagac gatgttctgg tactgcgcgt tattggcccg ctgttttttg ctgctgctga
    33721 aggcttgttc acggacctgg agtcacgtct tgaaggcaaa cggattgtga ttctgaagtg
    33781 ggatgccgtt ccggtacttg atgctggtgg tcttgatgcg ttccagcgtt ttgtgaagcg
    33841 tctgcccgaa ggatgtgaac tgcgcgtgtg caacgtggaa ttccagccac tgcgcactat
    33901 ggctcgcgca ggcattcaac cgatcccggg acgcctcgcg ttcttcccga atcgtcgcgc
    33961 ggcgatggcg gatttataag tacgagattg accagtcagc gcaaactgac tggtcagcaa
    34021 actgcattat ttgttagcta tgacggcgat tatcgctacg gcggcaacgt ttgtcatagt
    34081 gacgaaccca ccagtaacgg tcgcacacct gttcatgccc gccaacacgg gcacccgcca
    34141 gccagagaat ggcaccgacg aaaatactta gcactgcacc atgtgcgaaa tactggggca
    34201 tattaaactg tggtaactgg ttgaggattg aataccccac gccgaccacc attaccacca
    34261 gccccaaccc catgagcacg ttaccgagta acgaagcgtt tttgcgtttc atatgtcacc
    34321 tccggaactt tctgggttgt aacagggaat acccctcttc cttatgtgta aagtatagac
    34381 aacaccctgt gggttatgtg cgggcgtgat cacaattaca acccttattt caacaaaact
    34441 ttacaaataa acgcctgaac accttgactt tctaatagtc cactcaggca attattacgt
    34501 tatctgcatt tgatacgcag ttttttgtca agcggggccg caaccagtaa actacgcgcc
    34561 agttatgtac acactcagga caaaaaacgt gacgattaaa ttgattgtcg gcctggcgaa
    34621 ccccggtgct gaatacgccg caacgcgaca taatgctggt gcctggttcg ttgacttact
    34681 ggcagagcgt ttgcgcgctc cgctgcgcga agaggctaaa ttctttggtt atacttcgcg
    34741 agtcactctt ggaggcgaag atgtccgcct gttagtcccg actacattta tgaatctcag
    34801 cggcaaagcc gttgcggcga tggccagttt tttccgcatt aatccggacg aaattctggt
    34861 ggcccacgac gaactggatc tgcctcctgg cgtcgccaaa tttaaattgg gcggtggcca
    34921 tggtggtcac aatggactga aagacatcat cagtaaattg ggtaataacc ctaactttca
    34981 ccgtttacgc atcggaatcg gtcatcctgg cgataaaaat aaagttgtcg gttttgtgtt
    35041 aggcaaaccg cctgttagtg aacagaagtt aattgatgaa gccatcgacg aagcggcgcg
    35101 ttgtactgaa atgtggttta cagatggctt gaccaaagca acgaaccgat tgcacgcctt
    35161 taaagcgcaa taagtcgttg tctgcggcat ttttgccgag tgccgtgtat aataggcaaa
    35221 gttatttcca tttctgcaat ctgttagcaa taacagattg attattaaga tattaaggtg
    35281 atttaaatca tgggattcaa atgcggtatc gtcggtttgc ccaacgtcgg gaaatctacc
    35341 ctgttcaacg cgctgaccaa agccggtatt gaagcggcca actttccatt ctgcaccatt
    35401 gagccgaaca caggcgtcgt accaatgcct gaccctcgcc tggatcaact ggctgaaatc
    35461 gtaaaaccgc aacgtacgct tcccacgacc atggaatttg tcgatatcgc cggtctggta
    35521 aaaggcgcat cgaaaggcga aggtctgggt aaccagttcc tgaccaacat ccgtgaaacc
    35581 gaagcgatcg gtcacgttgt tcgctgcttt gaaaatgaca acatcattca cgtttccggc
    35641 aaagttaacc cggctgacga tattgaagtt atcaacaccg aactggcgct ggcagacctc
    35701 gacacctgcg aacgtgcgat tcatcgcgta cagaagaaag ccaaaggtgg cgataaagac
    35761 gcgaaagctg agctggcggt cctagaaaaa tgcctgcccc agctggaaaa cgcaggtatg
    35821 ctgcgcgcgc tggatttaag cgctgaagag aaagcggcta ttcgttacct gagcttcctg
    35881 acgctgaaac caacaatgta catcgccaac gtcaacgaag acggttttga aaacaaccca
    35941 tatcttgacc aggtgcgtga aatcgcggcg aaagaaggtt ctgttgtggt tccggtttgt
    36001 gctgctgttg aagcagacat tgccgaactg gacgacgaag aacgtgacga gtttatgcag
    36061 gagcttgggc tggaagaacc gggcctgaac cgtgtgatcc gtgtcggtta taagctgctg
    36121 aacctgcaaa cttacttcac cgctggggtg aaagaagtgc gtgcatggac cattccggtt
    36181 ggagcaaccg cgccgcaggc agcgggcaaa atccatactg attttgaaaa aggctttatc
    36241 cgtgcacaaa ccatctcgtt tgaagatttc atcacttaca aaggtgaaca aggcgcgaaa
    36301 gaagcaggca aaatgcgtgc agaaggtaaa gattacatcg ttaaagatgg cgatgtgatg
    36361 aacttccttt tcaacgtcta attaatttat tgtctcatga ggtttcgcta aatctcatga
    36421 agagcgcaaa agtatataaa atccacgcaa ttgcgtggat ttttcatttc accgagtcct
    36481 aatcattctt gtaacatcct acacttttgt gcttattgcc tacagtgaac cacgagaata
    36541 attaaagaca tcagtgtcag atgacggata tctcattagt agtgatataa cgtaagtttt
    36601 tgttcaacta cacatcaccc ccttcacagc tgatcaatat tgatcacgct ccaatatgct
    36661 ggcgagaaaa ggacttacct gaagggtaat atcccgcaga taaagtcccc gaataacgtg
    36721 attacgtgcg atcctggcaa gatgaaaatg ataaccagta gcacaataac aacgataggc
    36781 cagaaaaccc attgaccaaa aatgaatatc gatgtttttt gctttttctt attagtaatg
    36841 caaaaatggc gcatattgtt tttgttatgc agatacatca agcgaa
//
LOCUS       DAZPBM010000023        36839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPBM010000023 DAZPBM010000000
VERSION     DAZPBM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.023"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHUZQ_001054"
     CDS             complement(<1..716)
                     /locus_tag="ACHUZQ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1024809.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUZQ_001055"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUZQ_001055"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1024810.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUZQ_001056"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUZQ_001056"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1024811.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHUZQ_001057"
     CDS             complement(3650..4705)
                     /locus_tag="ACHUZQ_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1024812.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHUZQ_001058"
     CDS             complement(4715..5503)
                     /locus_tag="ACHUZQ_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1024813.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHUZQ_001059"
     CDS             complement(5521..6573)
                     /locus_tag="ACHUZQ_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1024814.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="ACHUZQ_001060"
     CDS             complement(6604..7446)
                     /locus_tag="ACHUZQ_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1024815.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="ACHUZQ_001061"
     CDS             complement(7433..8314)
                     /locus_tag="ACHUZQ_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1024816.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="ACHUZQ_001062"
     CDS             complement(8335..9645)
                     /locus_tag="ACHUZQ_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1024817.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="ACHUZQ_001063"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="ACHUZQ_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHUZQ_001064"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHUZQ_001064"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1024818.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHUZQ_001065"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHUZQ_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1024819.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHUZQ_001066"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHUZQ_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1024820.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHUZQ_001067"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHUZQ_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1024821.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHUZQ_001068"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHUZQ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1024822.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="ACHUZQ_001069"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="ACHUZQ_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1024823.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHUZQ_001070"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHUZQ_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHUZQ_001071"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHUZQ_001071"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1024824.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGCTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHUZQ_001072"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHUZQ_001072"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1024825.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHUZQ_001073"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHUZQ_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1024826.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHUZQ_001074"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHUZQ_001074"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1024827.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="ACHUZQ_001075"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="ACHUZQ_001075"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1024828.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="ACHUZQ_001076"
     CDS             21883..22047
                     /locus_tag="ACHUZQ_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024829.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="ACHUZQ_001077"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="ACHUZQ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1024830.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="ACHUZQ_001078"
     CDS             23556..23801
                     /locus_tag="ACHUZQ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1024831.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="ACHUZQ_001079"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="ACHUZQ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1024832.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="ACHUZQ_001080"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="ACHUZQ_001080"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1024833.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="ACHUZQ_001081"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="ACHUZQ_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1024834.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="ACHUZQ_001082"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="ACHUZQ_001082"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1024835.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="ACHUZQ_001083"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="ACHUZQ_001083"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1024836.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="ACHUZQ_001084"
     CDS             29464..29817
                     /locus_tag="ACHUZQ_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1024837.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="ACHUZQ_001085"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="ACHUZQ_001085"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1024838.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="ACHUZQ_001086"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="ACHUZQ_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="ACHUZQ_001087"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="ACHUZQ_001087"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1024839.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="ACHUZQ_001088"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="ACHUZQ_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHUZQ_001089"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHUZQ_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHUZQ_001090"
     CDS             complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHUZQ_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1024840.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHUZQ_001091"
     CDS             36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHUZQ_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1024841.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
BASE COUNT         8538 a         9770 c         9443 g         9088 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cacccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgccccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagt
//
LOCUS       DAZPBM010000024        36751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPBM010000024 DAZPBM010000000
VERSION     DAZPBM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.024"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUZQ_001092"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUZQ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1024842.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHUZQ_001093"
     CDS             complement(280..873)
                     /locus_tag="ACHUZQ_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1024843.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUZQ_001094"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUZQ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1024844.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHUZQ_001095"
     CDS             1788..2183
                     /locus_tag="ACHUZQ_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1024845.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUZQ_001096"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUZQ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1024846.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUZQ_001097"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUZQ_001097"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1024847.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUZQ_001098"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUZQ_001098"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1024848.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUZQ_001099"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUZQ_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1024849.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUZQ_001100"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUZQ_001100"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1024850.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUZQ_001101"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUZQ_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1024851.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHUZQ_001102"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHUZQ_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1024852.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHUZQ_001103"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHUZQ_001103"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1024853.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHUZQ_001104"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHUZQ_001104"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1024854.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUZQ_001105"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUZQ_001105"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1024855.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGCEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUZQ_001106"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUZQ_001106"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1024856.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUZQ_001107"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUZQ_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR1024857.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUZQ_001108"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUZQ_001108"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1024858.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUZQ_001109"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUZQ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1024859.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUZQ_001110"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUZQ_001110"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1024860.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUZQ_001111"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUZQ_001111"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1024861.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUZQ_001112"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUZQ_001112"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR1024862.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUZQ_001113"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUZQ_001113"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1024863.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUZQ_001114"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUZQ_001114"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1024864.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVDYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUZQ_001115"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUZQ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR1024865.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUZQ_001116"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUZQ_001116"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1024866.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHUZQ_001117"
     CDS             complement(26207..26548)
                     /locus_tag="ACHUZQ_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1024867.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUZQ_001118"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUZQ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1024868.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUZQ_001119"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUZQ_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1024869.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUZQ_001120"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUZQ_001120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1024870.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHUZQ_001121"
     CDS             33227..34570
                     /locus_tag="ACHUZQ_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1024871.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34667..34873)
                     /locus_tag="ACHUZQ_001122"
     CDS             complement(34667..34873)
                     /locus_tag="ACHUZQ_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1024872.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35198..35752
                     /locus_tag="ACHUZQ_001123"
     CDS             35198..35752
                     /locus_tag="ACHUZQ_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1024873.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35842..>36751)
                     /gene="ytfI"
                     /locus_tag="ACHUZQ_001124"
     CDS             complement(35842..>36751)
                     /gene="ytfI"
                     /locus_tag="ACHUZQ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR1024874.1"
                     /translation="LPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWIS
                     NYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLRG
                     SGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQA
                     IGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLGF
                     ALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSN
                     ITKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         8900 a         9847 c         9136 g         8868 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtattgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggctgcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agtccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    34681 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    34741 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    34801 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    34861 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    34921 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    34981 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    35041 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    35101 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    35161 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    35221 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    35281 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    35341 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    35401 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    35461 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    35521 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    35581 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    35641 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    35701 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    35761 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    35821 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    35881 aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    35941 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    36001 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    36061 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    36121 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    36181 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    36241 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    36301 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    36361 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    36421 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccgttatc
    36481 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    36541 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    36601 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    36661 aaaaaaattg tttgaatgtg tttttgtata cgatgacttt acaaataact ccaccgcacc
    36721 aataaaatta ttgtgtctga tcctgggtaa c
//
LOCUS       DAZPBM010000025        34267 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPBM010000025 DAZPBM010000000
VERSION     DAZPBM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.025"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(10..1032)
                     /locus_tag="ACHUZQ_001125"
     CDS             complement(10..1032)
                     /locus_tag="ACHUZQ_001125"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR1024875.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDGQTDPNDILHRASKLNPINKK
                     RIIHSVPGGTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCYGFGNGR
                     FGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELGKAIGQS
                     IKNHLE"
     gene            complement(1119..3416)
                     /locus_tag="ACHUZQ_001126"
     CDS             complement(1119..3416)
                     /locus_tag="ACHUZQ_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR1024876.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(3406..3681)
                     /locus_tag="ACHUZQ_001127"
     CDS             complement(3406..3681)
                     /locus_tag="ACHUZQ_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR1024877.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(3678..3902)
                     /locus_tag="ACHUZQ_001128"
     CDS             complement(3678..3902)
                     /locus_tag="ACHUZQ_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR1024878.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3905..4204)
                     /locus_tag="ACHUZQ_001129"
     CDS             complement(3905..4204)
                     /locus_tag="ACHUZQ_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR1024879.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(4204..4428)
                     /locus_tag="ACHUZQ_001130"
     CDS             complement(4204..4428)
                     /locus_tag="ACHUZQ_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR1024880.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(4492..4992)
                     /locus_tag="ACHUZQ_001131"
     CDS             complement(4492..4992)
                     /locus_tag="ACHUZQ_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR1024881.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(5162..5434)
                     /locus_tag="ACHUZQ_001132"
     CDS             complement(5162..5434)
                     /locus_tag="ACHUZQ_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR1024882.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            5571..5864
                     /locus_tag="ACHUZQ_001133"
     CDS             5571..5864
                     /locus_tag="ACHUZQ_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1024883.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5934..6914
                     /locus_tag="ACHUZQ_001134"
     CDS             5934..6914
                     /locus_tag="ACHUZQ_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1024884.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(7100..7600)
                     /gene="cpxP"
                     /locus_tag="ACHUZQ_001135"
     CDS             complement(7100..7600)
                     /gene="cpxP"
                     /locus_tag="ACHUZQ_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1024885.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7750..8448
                     /gene="cpxR"
                     /locus_tag="ACHUZQ_001136"
     CDS             7750..8448
                     /gene="cpxR"
                     /locus_tag="ACHUZQ_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1024886.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8445..9818
                     /gene="cpxA"
                     /locus_tag="ACHUZQ_001137"
     CDS             8445..9818
                     /gene="cpxA"
                     /locus_tag="ACHUZQ_001137"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1024887.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9924..10598)
                     /gene="yiiM"
                     /locus_tag="ACHUZQ_001138"
     CDS             complement(9924..10598)
                     /gene="yiiM"
                     /locus_tag="ACHUZQ_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1024888.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10747..11730)
                     /gene="kdgT"
                     /locus_tag="ACHUZQ_001139"
     CDS             complement(10747..11730)
                     /gene="kdgT"
                     /locus_tag="ACHUZQ_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR1024889.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11990..12610)
                     /gene="sodA"
                     /locus_tag="ACHUZQ_001140"
     CDS             complement(11990..12610)
                     /gene="sodA"
                     /locus_tag="ACHUZQ_001140"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1024890.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12895..13929
                     /gene="rhaT"
                     /locus_tag="ACHUZQ_001141"
     CDS             12895..13929
                     /gene="rhaT"
                     /locus_tag="ACHUZQ_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR1024891.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13926..14774)
                     /gene="rhaR"
                     /locus_tag="ACHUZQ_001142"
     CDS             complement(13926..14774)
                     /gene="rhaR"
                     /locus_tag="ACHUZQ_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1024892.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14848..15684)
                     /gene="rhaS"
                     /locus_tag="ACHUZQ_001143"
     CDS             complement(14848..15684)
                     /gene="rhaS"
                     /locus_tag="ACHUZQ_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1024893.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15972..17441
                     /gene="rhaB"
                     /locus_tag="ACHUZQ_001144"
     CDS             15972..17441
                     /gene="rhaB"
                     /locus_tag="ACHUZQ_001144"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1024894.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            17438..18697
                     /gene="rhaA"
                     /locus_tag="ACHUZQ_001145"
     CDS             17438..18697
                     /gene="rhaA"
                     /locus_tag="ACHUZQ_001145"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1024895.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18939..19763
                     /gene="rhaD"
                     /locus_tag="ACHUZQ_001146"
     CDS             18939..19763
                     /gene="rhaD"
                     /locus_tag="ACHUZQ_001146"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1024896.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19773..20087
                     /gene="rhaM"
                     /locus_tag="ACHUZQ_001147"
     CDS             19773..20087
                     /gene="rhaM"
                     /locus_tag="ACHUZQ_001147"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1024897.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            20388..20834
                     /locus_tag="ACHUZQ_001148"
     CDS             20388..20834
                     /locus_tag="ACHUZQ_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1024898.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            20845..22296
                     /locus_tag="ACHUZQ_001149"
     CDS             20845..22296
                     /locus_tag="ACHUZQ_001149"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1024899.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            22286..23356
                     /locus_tag="ACHUZQ_001150"
     CDS             22286..23356
                     /locus_tag="ACHUZQ_001150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1024900.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            23356..25104
                     /locus_tag="ACHUZQ_001151"
     CDS             23356..25104
                     /locus_tag="ACHUZQ_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR1024901.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(25154..26194)
                     /locus_tag="ACHUZQ_001152"
     CDS             complement(25154..26194)
                     /locus_tag="ACHUZQ_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR1024902.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(26347..27180)
                     /gene="fdhD"
                     /locus_tag="ACHUZQ_001153"
     CDS             complement(26347..27180)
                     /gene="fdhD"
                     /locus_tag="ACHUZQ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1024903.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            27374..30424
                     /gene="fdnG"
                     /locus_tag="ACHUZQ_001154"
     CDS             27374..30424
                     /gene="fdnG"
                     /locus_tag="ACHUZQ_001154"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:27959..27961,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1024904.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            30437..31339
                     /gene="fdoH"
                     /locus_tag="ACHUZQ_001155"
     CDS             30437..31339
                     /gene="fdoH"
                     /locus_tag="ACHUZQ_001155"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1024905.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            31336..31971
                     /gene="fdoI"
                     /locus_tag="ACHUZQ_001156"
     CDS             31336..31971
                     /gene="fdoI"
                     /locus_tag="ACHUZQ_001156"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1024906.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            31968..32897
                     /gene="fdhE"
                     /locus_tag="ACHUZQ_001157"
     CDS             31968..32897
                     /gene="fdhE"
                     /locus_tag="ACHUZQ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1024907.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(33214..33456)
                     /gene="yiiF"
                     /locus_tag="ACHUZQ_001158"
     CDS             complement(33214..33456)
                     /gene="yiiF"
                     /locus_tag="ACHUZQ_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1024908.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(33831..33947)
                     /locus_tag="ACHUZQ_001159"
                     /pseudo
     CDS             complement(33831..33947)
                     /locus_tag="ACHUZQ_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8490 a         8803 c         8567 g         8407 t
ORIGIN      
        1 gccattttat cattctaaat ggttcttgat tgattgccca attgcttttc ctagttccac
       61 aggaacagca ttacctatca ttttcccaat acttcttata acaaacttgt ttttgtcttc
      121 tacaaaaata taatccatcg ggaaagtttg caataaagca gcctctctaa gagaaatcgc
      181 tctatgctgt tctggatgtc caaaacgccc attcccgaaa ccataacaaa gagttgtaat
      241 tgttggactt ggcttatccc aagacatgcg cccatataca ctagcatacc ctttaccgct
      301 ggatttcata tgacaagcgg caaccaactc tttcggccag tccttccatg taccaccagg
      361 aacagagtgt ataattcttt ttttattaat cggatttaat ttactggctc tatgtaatat
      421 atcatttgga tctgtttggc catcttctaa tggaggaaga tttcctattg catctctaac
      481 tgttttcaaa ataacatcgt cacgttttac taactcaatc gcccccaatt ttgaggccaa
      541 caatacatga cgggaacggt tttgtgggat tccatattcg acacaatcaa tcttagacgc
      601 ccaaacataa tagcccaact ttaacaatga attataaaaa tcattataaa ctttatgttt
      661 agttacatca ggaacgttct ccattgttac gatttctgga gatacttcgc gaattaaacg
      721 ttcgaactca taaagaagag gccatttttt atcatcagcc ttatcttttc cttgagtata
      781 ttttgaaaaa ggttggcaag gtgcgcatcc tgccaatact tttacagacg catcaccata
      841 aagcctcagc agttcctctt ttgttacttt agcgatatct tgctcaataa acttagattt
      901 attgtttctt tcatagggaa acctacattc cccctcaagg tcaatgcctg caacgacatc
      961 aagccccgca agctgcaaac catgagttaa tccgccagca ccacaaaaaa ggtctatcac
     1021 ctttacagtc atcactcact ccaaatctat acgatactca gtatacatcc ctttctacaa
     1081 aatctcgagg cctaacttat aaactcatat ttattgagtt aacgcttcac tgagtttgac
     1141 tctaatactg ttgagaatcc cgaccactca tcagcgagcg ggtaagtgaa ttttttcccg
     1201 tcataattta cggttgcccc acgcgccagc acctcaagct cccatcgctg cggcctgata
     1261 ccgttctgag caaggtcaac gcggatacgg gtaatttgca ttcgttccga ccgggtcagt
     1321 ctggccgatg gcgctatttc atgcggtttt aacgggcttc cgtttctttg ctgacggttt
     1381 ggtcttctca gtccgtgttt taatgcacct ctgagcgccc tcacaacctc cgggtcattc
     1441 cattcgataa caccgtcatc taccagatta agcactgctg cggcgtgctc agaaggtgtg
     1501 ggagccggta acgaagtatc accaccagtg agctttccac agttattgac aggactccga
     1561 ggcgcggcga tgccgctttt taaagtcaaa ggctcaacga ccggcacttt cggaacaatg
     1621 cgccagtccg tcgttctggt gatatgaata tgacgcgcgc cgagatgcgg cgcgtaaatg
     1681 ccgaccactc tctcgacttc ttcctcgtac tcgttaactt catccgacgg actacgggcg
     1741 accctgacag tctgacaatc gcgcgggaca tttgccccac cctgcgcgct gatatacagc
     1801 gcaaaatcac cactgtctgc ggcggcgcgt gcagcctcga cgcgctcgtc aaactcatca
     1861 gcaatgctga cgccgcgagg caatttgcgt agttcacggt aagcccccat tgtcggcagg
     1921 ccaaccgttt taaattgcgg aatgcgccac gttgacgccc atgcggtaac agccgcggca
     1981 gtatctttca gcggtctgcc gctatcgtta tcgagctgcc catccagtgc atagccgtcg
     2041 atattttttg agatgtattt cgcgatatac cccgcagcac cgccccgatt aagatgtttt
     2101 gcctgaaaac ggtttcgcgc agctcctctt tcgtcgccat cctctttgag cgcgtagcga
     2161 cgcatgattt cgataatctg gttacgctgg cgtggattac aaaaaagcat catatgccag
     2221 tgcggcgttc cgtcgtggtg tggctcgacg actcgcaaac cgtagacctg taaatcatta
     2281 tccttgaatg ccgtgcgcat caggctccag atacggcaga gataacgctg cgcatccttt
     2341 ggattaaatg cctcatcgtt ccagccgtga tttagctgga cggttttact ttcgcctttt
     2401 ccaacctgac gtgtcgggtg atactttgac ggcgcggtca gcgtgataaa catccccaca
     2461 tcaccttctg cggcggcgta acgctcaata ccggcaatgg tgttcatcag ctccatccgg
     2521 cgaatttcag gattagaaat actgcccatc accttactga taaggtcgat gcgctcgcct
     2581 gtttccctgt tttcgagatc acacgattta agaaattcca gatttgcctg gcggcgtgca
     2641 cgcacatcac gaatggcatg tttactggca taaggagaac ggtctttatt gacctccccg
     2701 acagcaatca gtaacgcctc atgccagcgc atacgctggc ctttaagctg actaatccac
     2761 cactcatcgt taaacagacg ggcaatggca gaatatgcct gcctcgtggt catctgtcct
     2821 ttacggtatt ttttccagta gagaggggaa atattgaaag cacgtgcagc gccagcaaca
     2881 tgaccataga ggtgagcctg cgcctcatcc gtaaacagcg attctttttc gccatgcgca
     2941 tccacccagg catcgctgag ttcctcatac atcatgaaaa gctgcgatga gatacgggcg
     3001 gcaaactttt tcagctcctt gtcattcatt cccggcaggc gcgcatagtg gtcacgctca
     3061 gccagaaaca gcatcgacgc gtcggtgttc atttcatggc gctgattcac acgctcaatg
     3121 cgcggccata aacgacgctg aaaagtggat gtgaggaaat aaaacccgtg caccgggctt
     3181 ttattgcgcc ggatgtagtc atagcgtgac gtaaacagcg agcgcaaaaa gtaaggcagg
     3241 cggttaatcg tggataaaac accttgcacc tgacgcatct cgtcacgtgt aaggggtctt
     3301 tcgcgcccga cagcctcgcg tggcgcgttc catgcataag caccggtaaa cgccttaccg
     3361 gtgcctgcag caaatgctga cggagggaca aaacgcccgg aggctttaac ggccatatga
     3421 gccaaaagcc tctgaacaac gcttgctgag ttgctcaacc tgcgcgttta aatcagcaaa
     3481 agactttgcg cttccggtca gaatatcgtg atgcatcagg ccggaaacga gctggcttaa
     3541 tttcgggtaa taaccaacca ccgccagcca ttcctgaccg gcgtttttac cgctttccgc
     3601 tctctttttc tcgtggagaa taaactggaa gctgtcactg gtaacgacat aacgttcgcc
     3661 aatttcaata cgaatactca tgccgttctc cggtaatgtt tgttttttgc ttcaaagact
     3721 gactgacagg aaacacaacg cgtggctgac ggataagccg cacgacgggc agcaggaatt
     3781 ggcgcgtcac actcttcgca aaccagcgca gaagcaccgc aatgttttac ccttgccgcg
     3841 ttaatctggc gctccagtaa ttcagcctgt tgttcctgaa taaaatctat gttgtccggc
     3901 attatcagct ccttttatcg ttaagtttcc tggatacatc agcgcaataa ctggcaagtt
     3961 ctgtcgttaa ttttgtcagt tcatccactg aggaaatttg cttgtggaat acagcgcgtt
     4021 taacaagtaa attgaccaca tcagacagga ggtttaattc attctgataa atcgcgataa
     4081 cagattcagt gatgtcgcgt ttttctttat caagacaaag ttgaataaga gacaagtcgc
     4141 catttttcat aacggcgatt tttaaggcgt tattcagtaa tacaactgaa tgagaacagg
     4201 acatcaaagc acctccccgc gagacaatcc gatattgtga aatttttccg actcctgact
     4261 gagcagctcg actatctcca cgcgggataa ctccgccttt gtgatatggc gaatcatggc
     4321 gtcaagatga gaagaaaagc gcgtcgcagc gtcggcctgt gcttcggttc tggcctgttg
     4381 cagcagtaat gcgtatttac cgcactgatt ttcagaaact gtatgcatga ctttctccag
     4441 gcaaaaagaa gccccgcaca attaagtgcg ttaaaaactc tggttaatta tttaatgcaa
     4501 atattgctct ggttttaccg acgtcagaat tgtcggtgca tactcaaaca gactgaatag
     4561 ttcacgtaat gcacggaata aagcatcacg ccagtaacat gactcttcat taattcgcca
     4621 gtatggctga ttgaattctt tttcagtcaa tccggcatgc ataaataaag tacgacgctg
     4681 actgactgtt aaaaaactaa tatatgcata ctcacttgcg ccaacctgac ggcgttttga
     4741 gaatgcccca cgcaattcat caattgcaca aaccagccgt tcacgttcga cgtcgttcat
     4801 ttcttcaaaa cgcatcgttg cgtgacgttg ttttaactgc gcatgaaagc aaactgttag
     4861 ccgttcgcgc tccatcatct gattataata atcacatgta tcctgccaac gagggacggc
     4921 aagatgctta ccaattatcc ggcgcatagc tgctggctgt ttttcgacga gattgagcgt
     4981 catcactgtc atttccagac cctccggctt ttcagaaagg tcagagcctt ttttaacgga
     5041 ctctgttttt tggtgcggat aatgattccc ttgcgaccct tcccgtgggt gatggtgaag
     5101 tcaatcgccc tagggctttc gttacgcaat aactgagcaa tacaacgcgg ctcattcata
     5161 atcacaaccc catccacaaa agccatgcat cacgctgttc aaccggtcgg ttataaaacg
     5221 cctcacgtac agcgcgatta aactcaggaa tgaaaaccca tttttcaccg gcacgagcct
     5281 tcggtttgca aggatcacgc aattcaataa ttggtaattt atttgccttc accatttcac
     5341 tgacggctgt ctttggcttc cctaataaat cagcaaattt atccacatga accgcatcaa
     5401 gcggatactg aatcacataa ttttcagcgt ccatatatgg taccctcata ggatccagcc
     5461 ctttctaaac cactcaaaac cgtttagacg ctggtttatt ctcaaatcaa tggaacctat
     5521 ataggttcca gttttgaggg aatttagtcc ctatataggc accatgtcaa atgaaattaa
     5581 gcgaaaagat taaggccttg cgtgaggctg aagggctaag ccaatcaaaa ttctgtgaaa
     5641 tcatagagtt accgctaagc acacttaaaa aatatgaagg aggaaacttt gaacccggtg
     5701 gcacagcttt gctaaaaatc actatgcatc ccacattcca aaaatatgct ctatggctta
     5761 tgacagataa aaccgcgccg gacgcaggac aaatcgcacc ggctctcgcg cacattgggc
     5821 cagagtcaac agagtccaac cactccgcga aaaggattgg ctaactctat ataaagatta
     5881 cattttcacc atttgctacc aagatggtga atacagcgcc ggagggcttt cttatggcaa
     5941 ttaagaagct cgatgatggt cgctatgaag tggacattag acctcgcggt cgcgacggaa
     6001 aacgcatccg caggaaattc gaaagaaaag ctgaagcact agcatttgag cgatacacaa
     6061 tcgccaatgc cagtcagaaa gaatggggag gccagcgagc agaccgccgg actttgagtg
     6121 agttgctgga catctggtgg aaatatcacg ggcaaaacca cgagcatgga acaaaagagt
     6181 ttaatcatct actcaaaacc atcagcggca taggtgatat accagtgagc aggatgagca
     6241 aaagggcttt gatggattat cgttccatgc gactacgtga tggtatcagt gccgcaacga
     6301 taaaccgtga catgtacaga ttatccggca tgttcacaaa attaattcaa ttggatgaat
     6361 tttccgggca acacccaatt cacggactgc cgccactggc ggaggccaac cctgaaatga
     6421 cgttcctgga aaaagcagaa atcgaaaaac tgttaaatgt tttggctggt gatgacttac
     6481 ttgtcgcgct tttatgtctg agcactggag gaagatggac gaaagttgcc acgctaaaac
     6541 cagcacagat tacaaattgc agggttacct tcctgaaaac caaaaacggt aaaaagcgaa
     6601 ccgtgccgat ttctgaggaa ctggagaaaa aagttaaaga ggaggccagc gccaaattat
     6661 tcaaagttga ttatgagaaa ttttgcggga ttttacgcag agtgaaacct gatataccac
     6721 ccaatcaggc aacccacatc ctgcggcata cattcgcaag ccatttcatg atgaatgggg
     6781 gcaatataat cgcactgcaa cagattctgg gacatgcgag cattcagcag acgatggcct
     6841 atgcgcacct tgcgcctgac tatctgcaaa atgccgtcgc tctgaatcca ctaaaaggcg
     6901 gagtgacgtt ataaatttcc ccctctgagt gtccacatag tgtccacact caagaaattt
     6961 tgaaaccgct ccaaaccctt acagctttcg ataagtaact gtttttactt ttgattgcat
     7021 gtaagtattt gataaaaaaa accccacatc atgtggggga agacagggat ggtgtctatg
     7081 gcaaggaaaa cagggtttac tactgggaac gtgagttgct actactcaat agcttcaacg
     7141 atgaactttt ttgccattgc gtcacgtcac gcaactgctc cattcgttgt tgatgtttct
     7201 cgtttaaaac cgcttgctgc tccggcgtta acaggcgata catttggttg cggacttttg
     7261 ccatctcaac ctgacgagca atttgctcat tcgccatttt ttctgcctgt gcgcgcacag
     7321 cgttttcatc aaaattttct gcggtgacaa ggcgatgcat tgtctccagt tcgctaacat
     7381 taacaggagg ctgttcgtgc cgggcctgtt gcataagatc tcgcatctgc tgacgctgat
     7441 gttcggttaa acttatgccg tcgaacatat ggctctgcgt actgcgctgc gtaagttctt
     7501 cacccggatg ccagttatcg cctgaaccga cttcagcagc gtggcttaat gaactgactg
     7561 ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat catttgctcc caaaatcttt
     7621 ctgtcgcgat tcaacgatag agagtttacg attcaggctg caaacatgcg tcagggggtg
     7681 taaaacaacg taaagtcatg gattagcgac gtctgatgac gtaatttctg cctcggaggt
     7741 atttaaacaa tgaataaaat cctgttagtt gatgatgacc gagagctgac ttccctatta
     7801 aaggagctgc tcgagatgga aggcttcaac gtgattgttg cccacgatgg ggaacaggcg
     7861 cttgatcttc tggacgacag cattgattta cttttgcttg acgtaatgat gccgaagaaa
     7921 aatggtatcg acacattaaa agcacttcgc cagacacacc agacgcctgt cattatgttg
     7981 acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg agctgggcgc agatgactat
     8041 ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta ttcgcgcgat cctgcgccgt
     8101 tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt caccgacact ggaagttgat
     8161 gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg acgggcaaac gctggagtta
     8221 accggtactg agtttaccct gctctatttg ctggcacagc atctgggtca ggtggtttcc
     8281 cgtgaacatt taagccagga agtgttgggc aaacgcctga cgcctttcga ccgcgctatt
     8341 gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc gtaaagatgg tcacccgtgg
     8401 tttaaaacct tgcgtggtcg cggctatctg atggtttctg cttcatgata ggcagcttaa
     8461 ccgcgcgcat cttcgccatc ttctggctga cgctggcgct ggtgttgatg ttggttttga
     8521 tgttacccaa gctcgattca cgccagatga ccgagcttct ggatagcgaa cagcgtcagg
     8581 ggctgatgat tgagcagcat gtcgaagcgg agctggcgaa cgatccgccc aacgatttaa
     8641 tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc accgccagga cagcgtttgt
     8701 tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg cagcgaaatg cagatcattc
     8761 gtaactttat tggtcaggcc gataacgccg atcatccgca gaagaaaaag tatggccgcg
     8821 tggaactggt cggtccgttc tccgtgcgtg atggcgaaga taattaccaa ctttatctga
     8881 ttcgtccggc cagcagttct caatccgatt tcattaactt actgtttgac cgcccgctat
     8941 tactgctgat tgtcaccatg ttggtcagta cgccgctgct gttgtggttg gcctggagtc
     9001 tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga agttgcccag ggaaacttac
     9061 gccagcaccc ggaactggaa gcggggccac aggaattcct tgccgcaggt gccagtttta
     9121 accagatggt caccgcgctg gagcgcatga tgacctctca gcagcgtctg ctttctgata
     9181 tctctcacga gctgcgcacc ccgctgacgc gtctgcaact gggtacggcg ttactgcgcc
     9241 gtcgtagcgg tgaaagcaag gaactcgagc gtattgaaac cgaagcgcaa cgtctggaca
     9301 gcatgatcaa cgatctgttg gtgatgtcac gtaatcagca aaaaaacgcg ctggttagcg
     9361 aaaccatcaa agccaaccag ttgtggagtg aagtgctgga taacgcggcg ttcgaagccg
     9421 agcaaatggg caagtcgttg acagttaact tcccgcctgg gccgtggccg ctgtacggca
     9481 atccgaacgc cctggaaagt gcgctggaaa acattgttcg taatgctctg cgttattccc
     9541 atacgaagat tgaagtgggc tttgcggtag ataaagacgg tatcaccatt acggtggacg
     9601 acgatggtcc tggcgttagc ccggaagatc gcgaacagat tttccgtccg ttctatcgga
     9661 ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt ggggctggcg attgttgaaa
     9721 ccgccattca gcagcatcgt ggctgggtga aggcagaaga cagcccgctg ggcggtttac
     9781 ggctggtgat ttggttgccg ctgtataagc ggagttaaac tccgcatttg taggcaggat
     9841 aaggcgttta cgccgcatcc ggcatttgag caggatgcct gatgcgacgc tgatagcgtc
     9901 ttatcaggcc tacactccgg gggttatttt ccccacaacc ggcgggaaaa atcttcgatc
     9961 ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc ttttcgataa cccggcggcg
    10021 gagagtaaac ggtgatactg atcgtcatca aacggcatat gccatgcgat ggcggcagcc
    10081 tcctgcacgg tcacatcgct gacacgggaa accaactcca gcggcgcgtc cgcagacact
    10141 ttccctggcg ctatcacgct atacaaccac cccaccttac ctgtgttttg catcaactgc
    10201 gctatatcgc tgatatcaaa atggtaattg agtttatagc aaggcgagcg cggctggctg
    10261 acctgaatta atgcctctcc ccagcggaaa atatcgccca tatgaacatt actttccgtg
    10321 agaccgtcgg ttgagagatt ttcgccaaac gcaggcgcaa caaacaactc cgcctgttcc
    10381 ggaaattccc gcacccagta gagatagtgc tcacgaggat aatgacacag cgctctgtct
    10441 ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt ccagccccag ctccgtcagc
    10501 atcaactcac catcaacctg gattttggca attgcgctgg gtttgccttc gggataagcc
    10561 tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct gttccctctc cacgagaaat
    10621 ttccccatta gaccatattt attttatgtc aggcgtgctg ttttcgcggt aagtatccag
    10681 gcaggaactc aatagcaaac cggacgaacg ttagttcatc cggctgtggg gattaaggaa
    10741 gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt tgactttacg
    10801 tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg ccgtcgctac
    10861 cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg caatcagcac
    10921 aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag tgccatcgcc
    10981 accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa ttactgccac
    11041 acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa tggtattgcc
    11101 cagcgcgaag gcaaagaatg gaatcagcgt ttgcactgct ttgctgaaaa attctcgtaa
    11161 ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg cgccgacgaa
    11221 aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg tcatgagcgg
    11281 cccggactcc aacgacatca acacaaatgc cccagcttct tctttcgtac cgtactgctg
    11341 catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca gcgccagcgt
    11401 tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc gcgaggcaat
    11461 tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg atttacgcag
    11521 taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc acaccgccag
    11581 aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt tccccgcccc
    11641 cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca tcccccccgg
    11701 gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata tttttaaggg
    11761 ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata gacattcatt
    11821 taatcaataa aagtgatcaa aaccacatca attgaaacgc tgttttattt ttatcggatc
    11881 attgttttta ttttgaaatt gatcataaaa aaaccaccgc cgttggcgat ggttcatttt
    11941 ttaagctgat atgcggccta tacgcctcat tgcagcaggc ggcaaatgat tattttttcg
    12001 ccgcaaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct ttaatgtagt
    12061 ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc aggcccagaa
    12121 tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc tggttagcag
    12181 tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg gaaccaaagc
    12241 gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg aagtcacgtt
    12301 cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct ttccagaaca
    12361 ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg tctgctggca
    12421 gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt
    12481 ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg tggatttcca
    12541 tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg gatggcaggg
    12601 tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag
    12661 ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg tacttttcgt
    12721 aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca cttaatgccg
    12781 tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac agcacgattt
    12841 ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa gaagatgagt
    12901 aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc agcctgtttt
    12961 tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc agtcggtggg
    13021 attgtttcgt ggattattct gccgtggacc atcagcgccc tgttactacc ggatttctgg
    13081 gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt cggcgctatg
    13141 tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcat gtcgatggga
    13201 attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc aattatcaac
    13261 ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttgct cggcgttctg
    13321 gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga gcgcaagatg
    13381 ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt gatgtgcggc
    13441 attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca tgaagccgct
    13501 gccgcacttg gcgtcgatcc actgtatgtc gctctgccaa gctatgttgt catcatgggc
    13561 ggcggcgcga tcattaacct cggtttctgt tttattcgtc tggcaaaagt gaaggatttg
    13621 tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt gttactctcg
    13681 gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctgggg ccacgcccgc
    13741 attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta tgtattgtgc
    13801 ggcggtatcg tcgggctggt gctgaaagag tggaacaata caggacgccg tccggtaacg
    13861 gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg catcggcatg
    13921 gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat cccggtttcc
    13981 cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt cgaaatatca
    14041 ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct gacctgtcgc
    14101 agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa aacgcgctca
    14161 ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag gctagccgcc
    14221 aaccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa cgaatcactg
    14281 gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc agccatttcg
    14341 ttagcaaacg acacatgctg actactttca tgctcaagct gaccgataac ctgccgcgcc
    14401 tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc gctaaatccc
    14461 ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata aataatattc
    14521 tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg aatataaaag
    14581 agatcgccac gggtaatgcg ataaggacga tcgttgagta catgcagacc attaccgcgc
    14641 cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc ttgcggataa
    14701 cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt gagaagtttt
    14761 aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt cgcaatatgg
    14821 cgtacaaata cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc cctggcgaat
    14881 atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt tactgtcgct
    14941 gaatccacag cgataggcga tgtcagtaac gctggcctcg ctgtggcgta gcagatgtcg
    15001 ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg tttgctgctt
    15061 aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg catcccaatt
    15121 cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac gtgatgcgct
    15181 gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt gcataaacaa
    15241 gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt ccatctgtgc
    15301 aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag acggatactg
    15361 cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa atcgatccgg
    15421 cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca gatgccgatc
    15481 atgagcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc cattaaacac
    15541 atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat gatgttcagg
    15601 aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttaccag acggaaaaaa
    15661 atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg gcgaaatagt
    15721 aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa aaaacgtcga
    15781 tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc accacaatga
    15841 agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc cattttcctg
    15901 tcagtaacga gaaggtcacg aattcaggcg ctttttagac tggtcgtaat gaaattcagc
    15961 aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca tccagtgggc
    16021 gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc gaaatccatc
    16081 gttttaacaa tggcctgcat agccagaacg gttatgtcac ctggaatgtg gatagcctgg
    16141 aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt atcgatagca
    16201 ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt cagcgtgtgg
    16261 gcctgcccgt tgcttatcgc gatagccgct ccaatggcct aatggcgcag gcacagcaac
    16321 aactcggcaa acgcgatatt tatcaacgta gcggcatcca gtttctgccc ttcaatacgc
    16381 tttatcaact gcgtgcgctg acggagcaac aacctgaact tattccacac attgctcacg
    16441 ctctgctgat gccggattac ttcagctatc gcctgaccgg caagatgaac tgggagtaca
    16501 ccaacgccac gaccacgcaa ctggtcaata tcaatagcga cgactgggac gagtcgctac
    16561 tggcgtggag cggggctaac aaagcctggt ttggtcgccc gacgcatccg ggtaatgtca
    16621 ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt gccagccatg
    16681 ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgctgct tatctctctt
    16741 ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat gacacggcgc
    16801 tggcagccaa cataaccaat gaaggcgggg cggaaggtcg ctatcgggtg ctgaaaaata
    16861 ttatgggctt atggctgctt cagcgagtgc ttcaggagcg gcaaatcaac gatctcccgg
    16921 cgcttatcgc cgcgacacag gcacttccgg cctgccgctt catcatcaat cccaatgacg
    16981 atcgctttat taatcctgag gcgatgtgca gcgaaattca ggctgcgtgt cgggaaatgg
    17041 cgcaaccgct cccggaaagt gatgctgaac tggcgcgctg cattttcgac agtctggcgc
    17101 tgctgtatgc cgatgtgttg catgagctgg cgcagctacg cggtgaagat ttctcgcaac
    17161 tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgt gccgatgcct
    17221 gcggtattcg ggtgatcgcc gggcctgttg aagcctcgac gctcggcaat atcggcatcc
    17281 agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc gtcagcacca
    17341 ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac tatgtggcgc
    17401 agcttcactc gacacgacag acaaaggagc tttgcgcatg accactcaac tggaacaggc
    17461 ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg aggcgctgcg
    17521 ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg tttccggttt
    17581 tgaaaacccg gaaggttcgc tgaccggtgg gattcaggct actggtaatt atccgggcaa
    17641 agcgcgtaat gccagtgagc tacgagccga tctggaacag gctatgcggc tgattccggg
    17701 gccgaaacgg cttaatttac atgccatcta tctggaatca gacacgccag tctcgcgcga
    17761 ccagatcaaa cctgagcact tcaaaaactg ggttgaatgg gcgaaagcca atcagctcgg
    17821 tctggatttt aacccctcct gtttctcgca tccgctaagc gccgatggct ttacgctctc
    17881 gcatcccgac gacagcattc gccagttctg gattgatcac tgcaaagcca gccgtcgcgt
    17941 ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct ggatcccgga
    18001 tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc tggcagcact
    18061 ggatgaggtg atcagcgaga agctggatcc ggcgcaccat atcgacgccg ttgagagcaa
    18121 attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt acatggggta
    18181 tgccaccagc cgccagactg cgctgtgcct ggacgccggg catttccacc cgactgaagt
    18241 gatttccgac aagatttccg ccgccatgct gtatgtaccg cagttgctgc tgcacgtcag
    18301 ccgtccggtt cgctgggaca gcgatcacgt ggtgctgctg gatgatgaaa cccaggcgat
    18361 tgccagtgag attgttcgtc acgatctgtt cgaccgggtg catatcggcc tcgacttctt
    18421 tgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata tgaaaaaagc
    18481 cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag cggcgggcga
    18541 ttacactgcg cgtctggcgc tactggaaga acagaaatcg ttgccgtggc aggcggtctg
    18601 ggaaatgtat tgccaacgtc acgatacgcc agcaggtagc gaatggctgg agagcgtgcg
    18661 ggcttatgag aaagcgattt tgagccagcg tgggtaaaca ctgccggatg cggcgcgagc
    18721 gccttatccg gcctacaggt cgaatgacca tgcgtgctgc caccctcacc ccggccctct
    18781 ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg aacaccgctc
    18841 cggacagtcc cctcgcccct acggggagag ggttagggtg aggggaacaa gccgcaccat
    18901 gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt acccagtcct
    18961 ggtttgtcca gggaatgatc aaagccacca ccgatgcctg gctgaaaggc tgggacgagc
    19021 gcaatggtgg taacctgacg ctgcgcctgg atgacgccga tatcgcgcct tatcacggca
    19081 actttcatgc acaaccgcgc tatattcccc tcagccaacc catgccttta ctggcaaata
    19141 cgccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctt gatcctgcag
    19201 ctaacttagg cgtcgtaaaa gtcgacagcg acggcgcggg ctaccatatt ctctgggggt
    19261 taaccaacga agccgttcca acttccgaac ttccggctca cttcctttcc cactgcgagc
    19321 gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc accaacctga
    19381 tcgccctcac ctatgtactt gaaaacgaca ccgcggtctt tactcgccaa ctgtgggaag
    19441 gcagcaccga gtgtctggtg gtattcccgg atggcgttgg cattctgccg tggatggtgc
    19501 ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat tcgctggtgt
    19561 tgtggccctt ccacggcgtc ttcggcagcg ggtcgacgtt ggatgaaaca ttcggtttaa
    19621 tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg ggcggcatga
    19681 aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc gttacaccac
    19741 tagctagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt gtcatgcagg
    19801 ttaaccccga cgcccacgaa gagtatcagc gtcggcataa ccccatctgg ccggaactgg
    19861 aagcagtgtt gaaatctcac ggtgcgcata actacgccat ttacctcgac aaagcgcgta
    19921 atctgctgtt tgccacggta gagattgaat ctgaagaacg gtggaatgcg gtagccagca
    19981 ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct aacccggata
    20041 acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg cccaatgtgc
    20101 cagagatgcc tccggcacaa tattttaaat ttcgatttga aaaattaaac atattacatc
    20161 tcccggtaag ttatatttcc ctgatacatt gtgagtaaat cacaaaaata atgaataacc
    20221 cattaatgat tcatgtggtt tatttaaata accattatgt gcattactcc gcaaatctga
    20281 cctttcactc tgtctcaaat tgtcattgtt taccgacata tcgcctctca tcacttaatc
    20341 gctccaccat tacaattgat taaaatatat tttatggagt ccgattaatg gcagctctta
    20401 ctgcaagctg tattgacctg aatattcagg gcaatggcgc ttattccgtt ctgaagcagt
    20461 tggcgacaat agcgttacaa aacggtttta tcaccgactc acaccagttc ctgcaaaccc
    20521 tgctgctgcg cgaaaaaatg cactctactg gatttggttc cggtgtcgcc gtgccgcacg
    20581 gtaaaagcgc ctgcgttaaa caaccgtttg tattattcgc ccgcaaagcg caggctattg
    20641 actggaaagc cagcgatggc gaagacgtca attgctggat ctgcctcggc gtgccgcaaa
    20701 gcggcgaaga ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt atccaccagg
    20761 attttattca tcaactgcaa cagggcgacg ccgaccaggt gcttgccttg ttaaatcaaa
    20821 ccctcagctc ataaggaagt ggcgatggag tcatccttat gtattgtcgc gatcaccaac
    20881 tgccccgccg ggatcgctca cacctacatg gtggcggaag ccctggaaca gaaagcccgt
    20941 tctctcggcc ataccataaa agtggaaact caagggtcca gtggcgttga aaaccgctta
    21001 tccagcgaag agattgccgc tgccgattac gtcattctcg ctaccgggcg tggcctgagc
    21061 ggtgatgatc gcgcgcggtt tgccgggaag aaagtttatg agattgccat ctcccaggcg
    21121 ttgaaaaata tcgaccagat tttcagcgaa ttaccgacaa actcgcagct ttttgccgca
    21181 gatagcggcg tgaagcttgg caaacaggaa gtgcaaagcg gcagcgtgat gagccacctg
    21241 atggcgggcg tctctgcggc gctgccgttt gtcatcggcg gtggtatcct ggtggcgctt
    21301 gccaacatgc tggtgcagtt cggtttgccg tataccgata tgtcgaaagg cgcgccgtcg
    21361 tttacctggg tagttgaatc catcggttac cttggcttca cctttatgat ccccatcatg
    21421 ggcgcttata ttgcctcgtc gattgccgat aaaccggctt tcgctccggc gtttctggtt
    21481 tgctatctgg cgaacgacaa agcgctgctc ggcacccagt cgggtgcggg attcctcggc
    21541 gcggtggtgc tggggctggc gattggctat ttcgtcttct ggttccgcaa agtgcgcctt
    21601 ggcaaagcat tacaaccgct gctcggttca atgctgatcc cgttcgttac tctgctggtt
    21661 ttcggcgtgc tcacttacta cgttatcggg ccggtgatgt ccgacctcat gggcgggctg
    21721 ctccacttcc tgaataccat tcctccatca atgaagtttg cggcagcgtt tctggtcggt
    21781 gcgatgctgg cgttcgatat gggcggcccc atcaataaaa ccgcctggtt cttctgcttc
    21841 tcactgctgg aaaaacacat ttatgactgg tacgccatcg tcggcgttgt cgcgttgatg
    21901 ccgcccgttg cggcgggtct tgcgacgttc attgcgccca aactgtttac ccgacaggaa
    21961 aaagaggctg ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga accggctata
    22021 ccttacgctc tggcagctcc gttgccgatg attaccgcca atacgctggc gggtggcatt
    22081 accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg cacctggttt gggtatcttt
    22141 gacccgctga tcggcctgat gtcgccggtg ggttcgtttt atctggtgct agcgattggc
    22201 ctcgcgctga acatttcatt cattatcgtc ctgaaaggat tgtggttacg ccgcaaagcg
    22261 aaagccgcgc aacaggagct tgtccatgaa cattgagtta ctgcaacagt tgtgcgaagc
    22321 cagcgccgtc agcggcgatg aacaggaagt tcgcgacatt ctgataaaca cgctggaacc
    22381 ttgcgttaat gagatcacct ttgatggtct gggcagcttt gttgcccgta aggggaataa
    22441 aggtccaaaa gttgccgttg tcgggcatat ggatgaagtc ggctttatgg tcacccacat
    22501 cgacgagagc ggttttctgc gctttaccac cattggcggc tggtggaatc agtcaatgct
    22561 caaccaccgg gtaaccatac gcacacacaa gggatttaaa atccctggtg tgattggttc
    22621 cgtcgcacct catgcgttaa cggaaaagca aaagcaacag ccgctgtcat ttgatgagat
    22681 gttcattgat attggcgcga acagtcgcga tgaggtggaa aaacgcggtg tggcgatggg
    22741 cgattttatt agcccggaag ccaattttgc ctgctggggc gaagataaag tggtcggcaa
    22801 ggcgctggat aaccgcatcg gctgcgcaat gatggctgaa ctattgcaga cggtgaataa
    22861 tcccgaaatt acgctgtatg gcgtcggcag cgtggaagaa gaagttgggc tacgcggggc
    22921 acaaacctcg gcggaacaca ttaaaccgga tgtggtgatt gtgctggata ccgctgtcgc
    22981 aggtgatgtt ccgggcattg ataacattaa atacccgctg aaactgggcc aggggccggg
    23041 gctgatgctg tttgacaagc gctacttccc caaccagaaa ctggtggcgg cgttaaaaag
    23101 ctgtgccgca cataacgatt taccgctgca attttccacc atgaaaaccg gtgcgacgga
    23161 tggcgggcgc tacaacgtga tgggcggcgg gcgtccggtt gttgcgctgt gcctgccaac
    23221 ccgctatctg cacgctaaca gcggcatgat ttcaaaagcc gattacgatg ctctgctcac
    23281 gttgatacgg gattttctga ccaccttaac cgcggagaaa gtcaacgcgt ttagccagtt
    23341 ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga ttgtcgatct gctggagcaa
    23401 cagccgcgca cgcctggtga gctggcgcaa cagactggcg tttcaggcag gaccatcctg
    23461 cgtgatattg actatctcaa ctttaccctt aacggcaaag cccgcatttt cgccagtggc
    23521 agtgcgggct atcagctgga aatcttcgag cgccgcagct tttttcagtt gctgcaaaag
    23581 cacgataacg acgatcggct gctggcgctg ttattactga atacctttac tccccgtgcg
    23641 caactcgcct cggcgcttaa tttgccagaa acctgggtag cagagcgtct gccccggtta
    23701 aaacagcgtt atgaacgcac ttgttgcctg gccagtcgcc ccggtttggg ccatttcatt
    23761 gatgagacag aagagaaacg cgttatcttg ctggcgaact tgctgcgcaa agatccgttt
    23821 ttaattccgc tggcgggcgt aacacgagac aattttcagc atttatccac ggcctgcgac
    23881 aaccaacacc gctggccgct catgcagggt gattatctct ccagcctgat tctggcgatt
    23941 tacgccctgc gtaatcaact gaccgatgag tggccgcaat atcccggtaa cgagataaaa
    24001 caaatcgttg aacagagcgg catgtttctt ggtgataacg ctgtaagaac cctgacaggt
    24061 ttgatagaga aacagcatca gcaagcgcag gtaatttcag ccgatcatgt gctggggttg
    24121 ctgcaaaggg tgccaggcat cgcgtcattg aatattattg atacgcagct ggttgagaat
    24181 attaccgggc atttattacg ttgccttgcc gcaccggtgt ggattgccga gcaccgccaa
    24241 agcagcatga ataacctgaa agccgcctgg cctgcggcct ttgatatgag tctgcacttt
    24301 attacgctac tgcgtgaaca gctcgatatt ccccttttcg atagcgatct gatcggtttg
    24361 tattttgcct gtgcgctgga acggcatcaa aacgaacgcc agccgatcat tttgctctcg
    24421 gaccagaacg cgattgccac tattaatcag ctcgccattg agcgcgatgt tttacattgt
    24481 cgggtgatta ttgcccgtag cttaagcgag cttattgcca ttcgcgaaga gattgagccg
    24541 ttattgatca ttaacaacag ccattattta ctggatgacg cggtaaataa ttacatcacc
    24601 gtaaaaaata tcattacggc tgccgatatc gaacaaataa aacactttct ggcgacggca
    24661 tttattcgcc aacagccgga acgctttttc tctgcccccg gaagttttca ttattcgaat
    24721 gtacgcggtg aaagctggca acatattacc cggcaaattt gtgcgcaatt agtggcacaa
    24781 caccatatta ccgccgatga agcgcaacgg atcatcgccc gcgaaggcga aggtgaaaac
    24841 ctgattgtta atcgcctcgc catcccacat tgctggagcg aacaggagcg acgttttcgt
    24901 ggatttttta ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat taaccatgtc
    24961 ttgatcgcct gcgccgccgc cgatgcgcgt catgagctaa aaatatttag ctatctggca
    25021 agcgtattgt gtcagcatcc ggcagaagtt attgccgggt taacaggata tgaggcattt
    25081 atggagttac ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac actgcattta
    25141 tcatcaaaaa cgtttaacgc aggctattat agtcatcaac catttcgttg tactcgcgta
    25201 acgctcgtgg aaatgagtct tcgaccatcc agcttgtgtt tgcatcctga agatgctctt
    25261 tatcccagtc agagtacggg actttatctc tgacgcggcg aacatatttt ttagtctcga
    25321 gttgatactg gtctgccgaa ttaatgaacg agaaattcat accgccttta tccgcttctt
    25381 tggcctgcgc taccagcgtt tccagttcag agactttctt cattgccgca tcaacatcaa
    25441 acttctcttg cgaaatcagg ttattaattt gtttcgctga aatcatgact gccagagagt
    25501 agtagtggaa tgttttccct tctctttctt caatattttt cagttccgca agctggcgct
    25561 tatcattaat ttcctgaatc gccgcatgat aagattctgc tacaggttca aaggattcca
    25621 gattttttaa gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg tctttataat
    25681 tttcctgggt gtaatattta tccatttcgt taatggtatt acccaacgcc acggcagcat
    25741 caatataact caccgccacg ccatcaatag gttgcagcgc aggcgttaac gccacagcac
    25801 tttttacgcc tttttcacac tcagcgaggt tggattcact aatgccgtag atgccataaa
    25861 cagtactctc tttaccggta ggcccctgtt taaaatcttt cagccagtca gcataacgcg
    25921 ccagactgtt ctgtacaggg atctgtaact tgttgtagca cttgatatag acatccattt
    25981 tttcgtcgaa aaccgcttgt tcatcgacca ccggttgcgc aggtgtttca ggtttagctt
    26041 ctgcttttgc aacgggagct tcaggagctg gtgcggcagg ttgactattg gcaggcgggg
    26101 gcgtttctgt ttcggctttt ttatcatcac aacccgtcag accgagggcc atgatggcga
    26161 ctataatagc ggaagataat aaattccttt tcatttaatt gccttataaa tatatagata
    26221 agagagtgaa cataataaat gaaaaaagaa aacgtcacta cacgcgtttt atttataaat
    26281 aaaataataa aattcacgaa atgaataagt cctgcaattt aatatatatc gcaggactta
    26341 ttttaattaa ttgctcaaac gctgcggatg ggtataaacc gttgcccgac ccggtttaca
    26401 aaaacctacc agtgtcagat tacagcgctc agccacttct acagcaagcg tggtcgcggc
    26461 agacaccgca aacaaaatct ctacgccgca catcgccgac ttttgcacca tttcataact
    26521 ggcacgactg gaaaccagta ccgcaccttg ctgccagctt tccccttctt gtgaccggcg
    26581 gcctaacagt ttgtccagcg ccacatggcg acccacgtct tcatgcccgc cgaccagttc
    26641 gccagatggc aacatccagg cagcggcgtg agtacaacca gtcagttgcc ccactggctg
    26701 aaaatcgttg agatgacgta atgcatcatc cagtttgttg agatcaaacg tctgggtgaa
    26761 cggtagcggc tgcaccggtt ttccgatgtc attaagttgc tccacgccgc atacgccaca
    26821 gcccgtacgt cccgccagcg cccggcggcg ctccttcaac cccataaagc ggcggctgga
    26881 aagctcaatt tgtacttcaa gaccattaca ggaaggaacg acatccatgc cgaagatatc
    26941 gcgcggactt tctattatcc cctcggaaag cgaaaaaccg agcgcaaagt actcaaggtc
    27001 tttgggcgac gccatcatca ccacatgcga aatgccgttg tagaccagcg caacgggaac
    27061 ttcttccgcg acctcatcta accgtgggtg ttgtaaatca tcacgccgcc ataactcaat
    27121 ttgacgaaca cctgtgatat ttgtcacatt ttcgatttct tttcgctgtg tttttttcac
    27181 tttctcttaa ccagataaga acacacacac caacattatg gtattctgtt acaaaccctt
    27241 cctggatgga gggaaattga gccaattctg gacctttgcg gccccttccg caaagaaaaa
    27301 taactcccac tccctgcaca cgcagcaagc gaatgtaaat gggacgtgac aatgtcgaaa
    27361 caaggagcaa tccatgcagg tcagcagaag gcagttcttt aagatctgcg ctggcggtat
    27421 ggcaggcacc acggcagcgg cactgggttt tgcacccagc gtagcactcg cggaaacccg
    27481 gcagtataaa ctgctgcgca cccgcgaaac ccgtaatacc tgcacctatt gttccgtagg
    27541 ctgtgggctg ttgatgtaca gcctcggtga cggagcaaaa aacgccaaag catctatctt
    27601 ccatatcgaa ggcgatccgg atcacccggt caaccgcggt gcactttgtc cgaaaggcgc
    27661 tggcctggtg gatttcatcc actccgaaag ccgtctgaag tttccggaat accgtgcgcc
    27721 aggttctgat aaatggcagc aaatcagttg ggaagaggcg tttgatcgca tcgccaaact
    27781 gatgaaagaa gaccgcgatg ctaactacat tgcgcaaaac gccgaaggcg tgactgttaa
    27841 ccgctggctc tccaccggga tgctgtgtgc ttccgcgtcg agtaacgaaa ccggctattt
    27901 aacgcaaaaa ttctcccgcg cgctgggtat gctcgcggtc gacaaccagg cgcgtgtctg
    27961 acacggacca acggtagcaa gtcttgctcc aacatttggt cgcggtgcga tgaccaacca
    28021 ctgggtcgac atcaagaacg ccaacctcgt cgtggtgatg ggcggtaacg ccgctgaagc
    28081 tcacccggtc gggttccgct gggcgatgga agccaaaatt cacaatggcg cgaagctgat
    28141 tgtgatcgat cctcgcttta cgcgtacggc ggcggtggct gactactatg cccctattcg
    28201 ttccggtact gacattgctt tcctgtcagg cgtattgctg tacctgctga acaatgaaaa
    28261 attcaaccgc gaatacaccg aagcctatac caacgccagc ctgatcgtgc gtgaggatta
    28321 cggctttgaa gatggcctgt tcaccggcta tgacgcggaa aaacgcaagt acgataaatc
    28381 ctcctggact tacgaactgg acgaaaacgg cttcgccaaa cgcgatacca cgctgcaaca
    28441 tccgcgctgc gtgtggaact tgctgaaaca gcacgtttcc cgctacacgc cagatgtggt
    28501 tgaaaacatc tgtggtacgc caaaagacgc gttcctgaaa gtctgcgaat acatcgcaga
    28561 aaccagtgct cacgataaaa ctgcctcgtt cctgtatgcc ctcggctgga cgcaacactc
    28621 cgttggtgcg caaaacattc gtacgatggc gatgatccag ctgctgctcg gcaatatggg
    28681 gatggcaggc ggcggcgtta acgccctgcg cggtcactcc aatattcagg ggctgacgga
    28741 cctggggctg ctgtcgcaga gcctgccagg ttacatgacg ctgccaagcg agaagcagac
    28801 cgatctgcaa acctacctta ccgccaacac gccaaaaccg ctgctggaag gccaggtaaa
    28861 ctactggggc aactacccga aattcttcgt ctctatgatg aaggccttct ttggtgataa
    28921 agcgacggcg gaaaatagct ggggctttga ctggttgccg aagtgggata aaggctacga
    28981 cgtcctgcaa tacttcgaga tgatgaaaga gggcaaggtc aatggctata tctgccaggg
    29041 ctttaaccct gttgcctcat tcccgaacaa aaacaaagtg atcggttgtc tgtcgaaact
    29101 gaagttcctc gtcaccatcg acccgctgaa cactgaaacc tctaacttct ggcagaacca
    29161 cggtgagctg aacgaagttg actcgtcgaa gatccagacc gaagtgttcc gtctgccatc
    29221 gacctgcttc gcagaagaga acggttcaat cgttaactca ggtcgctggt tgcagtggca
    29281 ctggaaaggt gcggacgccc cagggattgc gctgactgat ggcgagatcc tctccggtat
    29341 cttcctgcgc ttgcgcaaga tgtatgccga acagggtggc gcaaacccgg atcaggtgct
    29401 gaacatgacc tggaactacg ccattccgca tgagccgaag tcggaagaag tggcgatgga
    29461 gagcaacggt aaggcgctgg ccgatattac cgatccggca accggtgcgg ttatcgtcaa
    29521 gaaaggccaa caacttagct cgttcgccca attgcgcgat gacggtacaa cctcctgtgg
    29581 ctgctggatt ttcgccggta gctggacgcc ggaaggcaac cagatggcgc gtcgtgataa
    29641 cgccgatccg tctggcctcg gtaacacgct gggctgggca tgggcatggc cgcttaaccg
    29701 ccgcattctg tataaccgcg cctccgcaga tccgcagggt aacccgtggg atccgaagcg
    29761 tcagttgctg aaatgggacg gcactaagtg gaccggctgg gatattccgg actacagcgc
    29821 agcgcctccg ggcagcggcg tcgggccgtt tatcatgcag caggaaggca tggggcgtct
    29881 gtttgccctc gataagatgg cggaaggtcc gttcccggaa cactacgagc cgtttgaaac
    29941 gccgctggga actaacccgc tgcatccaaa cgttatctcg aacccggctg cgcgaatctt
    30001 taaagacgac gccgaagcat tgggtaaagc cgataagttc ccgtatgtcg gaaccaccta
    30061 tcgtctgacc gagcacttcc actactggac caaacacgcg ctgttgaacg cgattttgca
    30121 accagagcag tttgtggaaa tcggggagtc gctggcgaat aaacttggca ttgcccaggg
    30181 cgataccgtg aaagtctcct ccaaccgtgg ctatatcaaa gccaaagcgg tggtgaccaa
    30241 acgtattcgc acgctgaaag cgaacggcaa agatatcgat accatcggta ttccgattca
    30301 ctggggctat gaaggcgtag ctaaaaaagg ctttattgcc aatacgttaa cgccattcgt
    30361 tggtgatgcg aacacgcaga cgccggagtt taagtccttc cttgtgaatg tggaaaaggt
    30421 gtaacggaga cgacatatgg cttatcaatc gcaagatatc attcgtcgtt ccgcgactaa
    30481 cggtctgacc cccgcgcctc aggcgcggga cttccaggaa gaagtggcga aactcatcga
    30541 cgtcaccacc tgtatcggct gtaaagcctg tcaggtggcg tgttcagagt ggaacgacat
    30601 tcgcgatacc gtcggcaata acattggggt gtacgacaac cccaatgatt taagcgccaa
    30661 atcgtggacg gtgatgcgct tctcggaagt ggagcagaac gacaaactgg aatggctgat
    30721 ccgcaaagac ggctgtatgc actgttccga tccaggctgc ctgaaagcgt gcccggcgga
    30781 aggtgcaatt attcagtatg ccaacggtat cgtcgacttc cagtccgagc agtgcattgg
    30841 ctgcggttat tgcattgcgg gctgtccgtt cgacattccg cgcctcaacc cggaagacaa
    30901 ccgcgtctac aaatgtacgc tgtgcgttga ccgcgtggtg gttgggcaag aaccagcctg
    30961 cgtgaagacc tgcccaacgg gcgcgattca cttcggtacg aaagagtcga tgaaaacgct
    31021 ggcgagcgag cgcgttgctg agctgaaaac ccgcggttac gacaatgcgg gtctgtacga
    31081 tccggcaggc gtcggtggta cacacgtcat gtacgtgctg caccatgctg acaagccaaa
    31141 tctgtatcat ggcttgccgg agaacccgga aatcagcgaa accgtgaaat tctggaaagg
    31201 catctggaaa ccgctcgcgg ctgttggctt tgcggctacc tttgcggcca gtatcttcca
    31261 ctacgtgggt gtcggtccga accgtgcgga tgaggaagag aataatctgc acgaagagaa
    31321 agacgaggag cgcaaatgaa acgacgtgac accatcgtgc gctacaccgc gccggaacgt
    31381 atcaaccact ggatcaccgc cttctgcttc atcctggcgg cggtgagcgg gctgggcttt
    31441 ttgttcccgt ccttcaactg gttgatgcaa atcatgggca caccgcagct ggcgcgaatt
    31501 ctgcacccgt ttgtcggcgt ggttatgttt gcctcgttca tcatcatgtt tttccgttac
    31561 tggcatcaca acctaatcaa tcgggatgat atcttttggg cgaagaatat tcgtaagatc
    31621 gtcgtcaacg aggaagtagg tgacaccggg cgttataact tcggtcagaa atgcgttttc
    31681 tgggcggcga ttattttcct ggttctgctg ctggtgagcg gcgtgattat ctggcgtcct
    31741 tattttgcgc ctgctttctc aatcccggtg atccgattcg cgttaatgct gcattcattt
    31801 gccgcagtgg cgttaattgt ggttatcatg gtgcatatct acgccgccct ttgggtgaaa
    31861 ggcacgatta ccgccatggt ggaaggatgg gttaccagcg cctgggcgaa gaaacatcac
    31921 ccgcgctggt accgtgaggt ccgcaagaca acggaaaaga aagctgaatg agtattcgca
    31981 taatcccgca agatgagctg ggttcgagcg agaaacgtac ggcggatatg attccgccgt
    32041 tattgttccc tcggctcaag aatttataca accgccgcgc cgagcgtctg cgcgagctgg
    32101 cagaaaataa tccgctgggt gattacctgc gctttgctgc gcttatcgcc cacgcccagg
    32161 aagtggtgct gtacgaccat ccgctggaga tggatctgac cgcgcgcatt aaagaagcca
    32221 gcgcacaggg caagcccccg ctggatattc acgttctgcc gcgtgataag cactggcaaa
    32281 agctgctgat ggcgctaatt gctgagctga aacctgaaat gagcggcccg gcgctggcag
    32341 tgattgagaa tctggagaag gcatcgactc aggagctgga agatatggcc agcgcgctgt
    32401 ttgcctctga tttctcgtcc gtcagcagcg ataaagcgcc gtttatctgg gctgcgctgt
    32461 cgctctactg ggcgcagatg gccaatctga tccccggcaa agcccgcgct gaatacggcg
    32521 aacaacgtca atattgcccg gtatgtggct ctatgccggt gtccagcatg gtgcaaattg
    32581 gcaccactca gggtctgcgt tacctgcact gcaacctgtg tgaaaccgaa tggcacgtag
    32641 tgcgcgtaaa atgcagcaac tgcgagcaga gcggcaaact gcattactgg tcgctggatg
    32701 acgaacaggc cgcgattaaa gccgaaagct gcgatgactg cggcacttac ctgaagattc
    32761 tttatcagga aaaagaaccg aaagttgaag ccgtggcaga tgacctcgcc tctctggtac
    32821 tggacgcgcg aatggagcaa gaaggctacg cccgtagttc cattaacccg ttcctgtttc
    32881 cgggtgaagg agagtaatct ctgacgtttt ccgggtggcg caggtcgccc ggattacagc
    32941 tcatagggtg tgacgacacc atctctcgta tttcaagatc atgccgcaat tgtctgtttg
    33001 cctcgttctt ccctccaaca catcgataag gttatgggta tgtcactgat gacaagcgta
    33061 tgattgcctg atgcgctgcg cttatcaggc ctaccaataa acttcaatat attgaatttg
    33121 catgactttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca acgcgcacgt
    33181 tgtcagcaat ctgattgaca tcattttttc ccattaccat tcctcgcgca gcttacgctg
    33241 atattcgaca ccatcttcct cacacccttg ccagatgcca ggaacactgg ttcttgctgt
    33301 ttgcgattga tttatcaggt attgatctac cgcttccctt aataggtccg cacgaggaag
    33361 attacgctgc acctcgagat catcaagttg cttaatcacc tcattcgata aatcgagtaa
    33421 aattctgccc atatctatac ctgccagaga gttcataaat atctcgccaa tatcattttt
    33481 gaatctatgg agagaaaagt acccttgccg aatctttaaa gaaagctcat ttacgcatca
    33541 ttttttattt ttgcgaggcg ttttccagaa ataataagag cgttgcagtt gtttcattta
    33601 ttactcaaaa atggtttagt tatcgcccag gatgagttat gggtgctatg aatatattat
    33661 attcagggat atttatttgc ataatctgaa tggtgtagcc gaaaaacgtc agcatcctaa
    33721 cagcaccagg atttaaggtg aaattaatct ttcatcacag accggtcagt actgccacca
    33781 accggtcttt acttgctcat cgttataatt ttgttggtaa cagttcggga aagttgtttt
    33841 tctaaattaa caacagaata actttcttgt ttaatgagat taatattggc aaccattata
    33901 gcctccttcc cacggtttaa gagcaaacat acaacataat gattcataac atacagagca
    33961 gtaaatgaat gatactgaca atgatgccag cccattatct ttgcataatg tttgtgagct
    34021 atagatttta aaaatattta tgagaaaagt atggcaccag aggaggtttt gactatcaaa
    34081 aatcgctcaa acctgaacta agatgatgag ttaagcatta tctgctataa ttgccactca
    34141 gctctacaaa taatatttaa atgctcctga acagtgatgt agcaagacac gtatggtgcc
    34201 aacgaagaat aaaaatttat agagttctta ttcaatatta ccggcataga cagggaatga
    34261 tgtaaat
//
LOCUS       DAZPBM010000026        33529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPBM010000026 DAZPBM010000000
VERSION     DAZPBM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.026"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            60..761
                     /locus_tag="ACHUZQ_001160"
     CDS             60..761
                     /locus_tag="ACHUZQ_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1024909.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            786..3385
                     /locus_tag="ACHUZQ_001161"
                     /pseudo
     CDS             786..3385
                     /locus_tag="ACHUZQ_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3376..4446
                     /locus_tag="ACHUZQ_001162"
     CDS             3376..4446
                     /locus_tag="ACHUZQ_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1024910.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4458..5000
                     /locus_tag="ACHUZQ_001163"
     CDS             4458..5000
                     /locus_tag="ACHUZQ_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1024911.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5008..5523
                     /locus_tag="ACHUZQ_001164"
     CDS             5008..5523
                     /locus_tag="ACHUZQ_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1024912.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5489..6226
                     /locus_tag="ACHUZQ_001165"
     CDS             5489..6226
                     /locus_tag="ACHUZQ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1024913.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6337..7347
                     /gene="pyrD"
                     /locus_tag="ACHUZQ_001166"
     CDS             6337..7347
                     /gene="pyrD"
                     /locus_tag="ACHUZQ_001166"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1024914.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7521..8063
                     /gene="zapC"
                     /locus_tag="ACHUZQ_001167"
     CDS             7521..8063
                     /gene="zapC"
                     /locus_tag="ACHUZQ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1024915.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8060..9169)
                     /gene="ycbX"
                     /locus_tag="ACHUZQ_001168"
     CDS             complement(8060..9169)
                     /gene="ycbX"
                     /locus_tag="ACHUZQ_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1024916.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            9413..11521
                     /gene="rlmKL"
                     /locus_tag="ACHUZQ_001169"
     CDS             9413..11521
                     /gene="rlmKL"
                     /locus_tag="ACHUZQ_001169"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1024917.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11533..13440
                     /locus_tag="ACHUZQ_001170"
     CDS             11533..13440
                     /locus_tag="ACHUZQ_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1024918.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            13570..14823
                     /gene="pqiA"
                     /locus_tag="ACHUZQ_001171"
     CDS             13570..14823
                     /gene="pqiA"
                     /locus_tag="ACHUZQ_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1024919.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14828..16468
                     /gene="pqiB"
                     /locus_tag="ACHUZQ_001172"
     CDS             14828..16468
                     /gene="pqiB"
                     /locus_tag="ACHUZQ_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1024920.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16465..17028
                     /gene="pqiC"
                     /locus_tag="ACHUZQ_001173"
     CDS             16465..17028
                     /gene="pqiC"
                     /locus_tag="ACHUZQ_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1024921.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17284..17451
                     /gene="rmf"
                     /locus_tag="ACHUZQ_001174"
     CDS             17284..17451
                     /gene="rmf"
                     /locus_tag="ACHUZQ_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1024922.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17521..18039)
                     /gene="fabA"
                     /locus_tag="ACHUZQ_001175"
     CDS             complement(17521..18039)
                     /gene="fabA"
                     /locus_tag="ACHUZQ_001175"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1024923.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18108..19868)
                     /locus_tag="ACHUZQ_001176"
     CDS             complement(18108..19868)
                     /locus_tag="ACHUZQ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1024924.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20054..20506
                     /gene="matP"
                     /locus_tag="ACHUZQ_001177"
     CDS             20054..20506
                     /gene="matP"
                     /locus_tag="ACHUZQ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1024925.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20582..21622)
                     /gene="ompA"
                     /locus_tag="ACHUZQ_001178"
     CDS             complement(20582..21622)
                     /gene="ompA"
                     /locus_tag="ACHUZQ_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1024926.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(21977..22486)
                     /gene="sulA"
                     /locus_tag="ACHUZQ_001179"
     CDS             complement(21977..22486)
                     /gene="sulA"
                     /locus_tag="ACHUZQ_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1024927.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            22705..23334
                     /gene="sxy"
                     /locus_tag="ACHUZQ_001180"
     CDS             22705..23334
                     /gene="sxy"
                     /locus_tag="ACHUZQ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR1024928.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23297..25459)
                     /gene="yccS"
                     /locus_tag="ACHUZQ_001181"
     CDS             complement(23297..25459)
                     /gene="yccS"
                     /locus_tag="ACHUZQ_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1024929.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(25469..25915)
                     /locus_tag="ACHUZQ_001182"
     CDS             complement(25469..25915)
                     /locus_tag="ACHUZQ_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1024930.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26038..28092
                     /gene="helD"
                     /locus_tag="ACHUZQ_001183"
     CDS             26038..28092
                     /gene="helD"
                     /locus_tag="ACHUZQ_001183"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1024931.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28124..28582)
                     /gene="mgsA"
                     /locus_tag="ACHUZQ_001184"
     CDS             complement(28124..28582)
                     /gene="mgsA"
                     /locus_tag="ACHUZQ_001184"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1024932.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28678..29340)
                     /locus_tag="ACHUZQ_001185"
     CDS             complement(28678..29340)
                     /locus_tag="ACHUZQ_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1024933.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29513..29926
                     /locus_tag="ACHUZQ_001186"
     CDS             29513..29926
                     /locus_tag="ACHUZQ_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1024934.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29971..30288)
                     /gene="hspQ"
                     /locus_tag="ACHUZQ_001187"
     CDS             complement(29971..30288)
                     /gene="hspQ"
                     /locus_tag="ACHUZQ_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1024935.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30346..31536)
                     /gene="rlmI"
                     /locus_tag="ACHUZQ_001188"
                     /pseudo
     CDS             complement(30346..31536)
                     /gene="rlmI"
                     /locus_tag="ACHUZQ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            31631..31909
                     /gene="yccX"
                     /locus_tag="ACHUZQ_001189"
     CDS             31631..31909
                     /gene="yccX"
                     /locus_tag="ACHUZQ_001189"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1024936.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31906..32235)
                     /gene="tusE"
                     /locus_tag="ACHUZQ_001190"
     CDS             complement(31906..32235)
                     /gene="tusE"
                     /locus_tag="ACHUZQ_001190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1024937.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32326..32985)
                     /gene="yccA"
                     /locus_tag="ACHUZQ_001191"
     CDS             complement(32326..32985)
                     /gene="yccA"
                     /locus_tag="ACHUZQ_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1024938.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33192..33279)
                     /locus_tag="ACHUZQ_001192"
     tRNA            complement(33192..33279)
                     /locus_tag="ACHUZQ_001192"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33243..33245),aa:Ser,seq:tga)
BASE COUNT         8650 a         8013 c         8762 g         8104 t
ORIGIN      
        1 aattagtacc cctctagtgt tctggagggg atattcatat tttttaagag tgactattta
       61 tgaaaacttg cataacaaag ggaattgtga ccgtaagttt aacggcaata ttactcagct
      121 gctcatccgc atgggcggca ggtaaaggcg ggattggact tgcagcaaca cgtcttgttt
      181 attcagaagg tgaagagcaa atttcactgg gtgtacgtaa caccagtccg gatgttcctt
      241 atcttattca gtcatgggtg atgaccccag ataataaaaa atcagcagat tttattatta
      301 caccaccgct atttgtgctg aatccggcta atgagaatct gttacgcatt atgtacattg
      361 gtgcgccgtt ggcgaaagac agagaaaccc ttttcttcac tagcgtacgg gcagtccctt
      421 caacaacgaa gcgggaagag ggaaataccc tgaagattgc cacacaaagc gtcatcaaac
      481 ttttctggcg accaaaaggt ttagcgtatc ccttaggcga ggctccggcg aaactgcgtt
      541 gcacttcgtc agctgacatg gttacggtca gtaacccaac accttatttc attaccctga
      601 cagacctgaa aataggggga aaggtagtta aaaatcaaat gatttccccc tttgataaat
      661 accaattctc tctgccaaag ggggccaaaa atagcagcgt aacgtatcga accatcaatg
      721 attacggggc ggaaacgcca caactcaact gtaaatcgta agccgtcttc agttaagaga
      781 gcgagatgta tagaactcat cgacaacaca gcctgttaag ctctggtgga gtgccatcgt
      841 ttattggtgg gctggtggtg tttgtgtcgg cagcgttcaa tgcacaagct gaaacctggt
      901 tcgatcctgc ttttttcaaa gatgatccct caatggtggc cgatttgtct cgtttcgaaa
      961 aaggacaaaa aataacgcca ggggtttatc gtgtcgatat tgttctgaat cagacaattg
     1021 tagatacgcg caacgtcaat tttgttgaga taacgccaga gaaggggatt gccgcctgtt
     1081 tgacgactga aagcctggat gcaatgggtg ttaatactga tgcgtttccg gcttttaaac
     1141 aactggacaa acaagcgtgt gtgccattgg cggagattat tccggatgcc agcgtaacgt
     1201 ttaatgtgaa taaactccgt ctggaaattt cagtaccgca aatcgccatc aaaagtaatg
     1261 cccgtggtta tgtcccccct gaacgttggg atgaagggat caacgcgcta ttactgggat
     1321 attcgtttag cggggctaac agtattcata gcagcgcaga cagtgattct ggcgacagct
     1381 attttctgaa tttaaacagt ggcgttaatt taggcccatg gagattgcgc aacaattcaa
     1441 catggagtcg cagtagtggc caaaccgcag aatggaagaa tctcagcagc tatttgcagc
     1501 gggcggttat tccactaaaa ggcgaactga ccgtaggtga tgattatact gcaggcgatt
     1561 ttttcgatag tgtcagcttt cgtggtgtgc agctggcgtc agatgacaac atgctgccag
     1621 acagcctgaa agggtttgcg cctgtggtgc gtggtatcgc caaaagcaat gcccagataa
     1681 cgattaagca aaatggttac accatttacc aaacttatgt atcgcctggt gcttttgaaa
     1741 ttagtgatct ctattccacg tcgtcgagcg gtgatttgtt agttgaaatc aaagaagcgg
     1801 acggcagcgt caatagctac agcgtaccgt tttccagcgt gccattactc cagcgtcagg
     1861 ggcgaatcaa atacgcggtg acactggcga aatacagaac caatagtaat gaacagcagg
     1921 agagcaaatt tgcctaggcc acgttgcagt ggggcggacc gtggggaacg acatggtatg
     1981 gtggtggaca atatgctgaa tattaccgtg ccgccatgtt tggtctggga tttaaccttg
     2041 gcgatttcgg agcaatttcg ttcgatgcga cccaggcgaa gagtacgctg gcagaccaaa
     2101 gcgaacataa aggtcagtca tatcgttttc tgtatgccaa aacgctcaac caattgggca
     2161 ctaattttca attgatgggc tatcgctatt cgacgtcggg tttctacacc ctttccgaca
     2221 ccatgtataa acatatggat ggctacgaat ttaatgacgg tgatgatgaa gatacgccga
     2281 tgtggtcgcg ttattacaat ttgttttaca ccaaacgtgg caaactgcag gtcaatatct
     2341 cccagcaatt aggcgagtac ggttcgtttt atttaagtgg tagccagcaa acttactggc
     2401 ataccgatca acaggatcgg ctattacagt ttggctacaa cacgcaaatt aaagatctct
     2461 cgctgggggg ttcctggaac tacagtaagt cccgtggtca acctgatgct gatcaggtgt
     2521 ttgcactaaa tttttccctg ccgctcaatc tgttgctccc cagaagtaat gatagctata
     2581 ccaggaaaaa aattacgcct ggatgacctc taacaccagc atcgataacg aagggcacat
     2641 tacacaaaac ctgggtttaa cggagacact actcgatgac ggtaacctga gctacagcgt
     2701 gcaacaggga tataacagcg aggggaaaac ggctaatggt agcgccagca tggactacaa
     2761 aggggcgttt gcagatgccc gagtgggcta caactacagc gataacggca gtcaacaaca
     2821 actgaactac gctctttcag gcagtttagt tgcccattca cagggcatta ccctggggca
     2881 atcgctgggg gaaactaacg tgctgattgc agcaccaggc gcagagaata ctcgtgtggc
     2941 gaacagcacc gggctgaaaa ctgactggcg cggatatacc gttgtcccct atgccacttc
     3001 ttatcgggaa aatcgaatcg cacttgatgc ggcatcgtta aaacgtaacg tggatcttga
     3061 aaatgcagta gtcaacgtgg ttcccaccaa aggggcgttg gttctggcgg agttcaatgc
     3121 ccatgcggga gcaagggtat taatgaaaac atcaaagcag ggtataccgc tgcgttttgg
     3181 cgcgatagcg acgctggacg gcgtacagac taatagcggc attattgatg atgatggctc
     3241 gctctatatg tccggtttgc ccgctcaggg agcgataact gtacgctggg gcgaagctcc
     3301 cgatcaaatt tgtcatatca gttaccagct taccgaacaa caaattaact ctgcgattac
     3361 gcgaatggat gccatatgca gataatcttt ggagaaaaat gcgtgttatt actacgacta
     3421 ttttttgccg ccgtcttaat gctatggtgc gctcaaactg ctgcttatag cgggcagtgt
     3481 cataccactc aggggaatcc gtatattggc gtcaattttg gcgttaaaac cctggaggaa
     3541 gaagaaaata cggctggagt agttaaagac aaattttatc agtggaacga atcgaatgat
     3601 tattatgttt cctgtgattg cgataaagac aatgtcagaa gtggccgatg ggcattcgcc
     3661 gcggattcac cgttagtcta tttaggcgac aactggtaca aaattaatga ctatcttgcc
     3721 gccaaagttt tattgcaggt taaaggcagt tctcctactg cggttccttt cgaaaacgtg
     3781 ggcacagggg cagatacacg atggcatatt tgcgatcccg gcggtcaacg tttaggtggc
     3841 cagggggcta gcggtaatag cggtagcttt tccctgaaaa tattgcagcc gttcgttggt
     3901 tcggtcgtca ttcctcctat ggcgctggcg cgattatttg aatgctacaa catacccgca
     3961 ggtgattcct gcacgactac aggtacaccg gttttagtgt attacctgtc tggtacgatc
     4021 aattcacttg gctcatgttc cgtcaatgcc ggagaaacaa tcgaggtcga tctgggcgac
     4081 gcatttgcgg ctaactttcg tgttgtaggg cataagcctc ttggggccag aacggcagaa
     4141 cttgcaattc cagtcaggtg taacacggga aacgcggggt tagttaacgt caacctgagt
     4201 ctgacggcaa ccacagaccc cagctatccc caggcgatta agacgtcacg tcctggcgtg
     4261 ggcgtggtgg tgaccgatag ccagaacaac attatttccc ctgctggtgg aacattaccg
     4321 ctctctattc ctgatgatgc agacagtatc gcgcgaatga atgtctatcc agtcagcacg
     4381 acaggtgtac caccagaaac cgggcgattt gaagccacgg caacggtgag aataaatttt
     4441 gattaacgcg tgaacgtatg aagaaaaaaa cgatatttca gtgcgttatc ttgttcttta
     4501 gcattcttaa catccatgtc gggatggctg ggcctgaaca agttagtatg catatttatg
     4561 ggaatgtggt cgatcagggc tgtgatgtcg ccaccaaaag tgcattacaa aatattcata
     4621 ttggtgattt taatatcagt gattttcagg ccgcgaatac cgtaagcact gctgctgatt
     4681 tgaatatcga tatcaccggt tgtgccgcta gtattactgg cgcggacgtc ctttttagcg
     4741 gcgaggctga cacccttacg ccgacactgc tcaaactaac tgacacaggc ggaagcggtg
     4801 gtatggcaac ggggattgcc gtgcaaattc ttgatgcgca aagtcagcaa gaaatcccgc
     4861 tcaatcaggt ccagcctctt acgcccttaa aagccgggga taacacactc aaatatcaac
     4921 ttcgttataa gtccacaaag gcgggagcaa cgggcggtaa tgcgacggcg gttctctatt
     4981 ttgatctggt ttaccagtga aggagtgatg ttgaaacgga taatctggat tctgttctta
     5041 ctgggattaa cggggggctg tgagctattt gcccatgatg gcacggtcaa cattagcgga
     5101 tcgtttcgcc gtaatacatg cgtgctggca caggatagca agcaaatcaa tgtgcagtta
     5161 ggcgatgtct cgctgacacg tttttttcat gggaattatg gcccggagaa atctttcatc
     5221 attaatttgc aggattgcgg aacagatgtc agcacggttg acgtcacctt ttcaggaacc
     5281 cccgacggag tgcagagcga aatgctgtcc attgaaagtg gtacggatgc agccagcggg
     5341 ctggcaattg cgattctgga tgatgcgaag atattgattc cgcttaacca ggcgagtaaa
     5401 gactacagcc tacatagcgg taaggtgcca ttaacttttt atgcgcaatt gcgacctgtc
     5461 aatagcgatg tgcagtcggg taaagtgaat gccagcgcga catttgtact tcattatgac
     5521 taatacgtgg aacagattgg cgctcttgat tttcgccgtt ttatcgctgc tggtggcggg
     5581 tgaattgcgg gcaggagtgg tggtcggcgg aacgcgattt atctttccgg cagacagaga
     5641 atcgatatct attttactga ctaataccag tcaggaatcc tggcttatta acagtaaaat
     5701 caaccgccca acgcgttggg ccggggggga agcgtcgaca gtgccagcac cattactggc
     5761 cgctccgcca cttattctcc tgaagcccgg tactacaggc acgttgcgct tgctgagaac
     5821 ggaaagcgac atcttgcctg tggatcgcga aacgctattt gagttaagca ttgccagcgt
     5881 gccatccggc aaagttgaaa atcagagcgt aaaagtggcg atgcgctcgg tatttaaact
     5941 gttctggcga cccgaaggtt tgccgggcga cccgctggaa gcttatcaac aattacgctg
     6001 gacacggaat tcgcagggtg tacaactcac taacccaacg ccttactaca ttaacctgat
     6061 tcaggtgagc gtaaatggta aagcgttaag taatgcggga gttgtgccgc ctaaaagcca
     6121 gcgtcagaca agctggtgtc aggcaatcgc accgtgtcat gtcgcctggc gggcgataaa
     6181 tgattacggc gggttgagtg caaagaagga gcaaaatctg ccctgaaaca ggttcggaaa
     6241 acgtttgcgt tttttttgcc gcaggtcaat tcccttttgg tccgaactcg cacataatac
     6301 gcccccggtt tgcacaccgg gaatccagga gagttcatgt actacccctt cgttcgtaaa
     6361 gcccttttcc agctcgatcc agagcgcgct catgagttta cttttcagca attacgccgt
     6421 attacaggaa cgccgtttga agcactggtg cggcagaaag tgcctgcgaa acctgttaac
     6481 tgcatgggcc tgacgtttaa aaatccgctt ggtctggcag ccggtcttga taaagacggg
     6541 gagtgcattg acgcgttagg cgcgatggga tttggatcga tcgagatcgg taccgtcacg
     6601 ccacgtccac agccaggtaa tgacaagccg cgtctctttc gtctggtaga tgccgaaggt
     6661 ttgatcaacc gtatgggctt taataatctt ggcgttgata acctcgtaga gaacgtaaaa
     6721 aaggcccatt atgacggcgt cctgggtatt aacatcggca aaaataaaga tacgccagtg
     6781 gagcagggca aagatgacta tctgatttgt atggaaaaaa tctatgccta tgcgggatat
     6841 atcgccatca atatttcatc gccgaatacc ccaggattac gcacgctgca atatggtgaa
     6901 gcgctggatg atctcttaac cgcgattaaa aataagcaaa atgatttgca agcgatgcac
     6961 cataaatatg tgccgatcgc agtgaagatc gcgccggatc tttctgaaga agaattgatc
     7021 caggttgccg atagtttagt tcgccataat attgatggcg ttattgcaac caataccaca
     7081 ctcgatcgtt ctcttgttca gggaatgaaa aattgcgatc aaaccggtgg cttaagtggt
     7141 cgtccgcttc agttaaaaag caccgaaatt attcgccgct tgtcactgga attaaacggt
     7201 cgcttaccga tcatcggtgt tggcggcatc gactcggtta tcgctgcgcg tgaaaagatt
     7261 gctgcgggtg cctcactggt gcaaatttat tctggtttta tttttaaagg tccgccgctg
     7321 attaaagaaa tcgttaccca tatctaatta tttcttcgaa ttcctcttta taagataatc
     7381 agggctttat tttcagccct ggttgtttta tattcatcgc tgttgcttat ttagacattt
     7441 tgtactttta ttgttgaggt tattaagcga agcgacaatg gattgtgttg ctgcggtttt
     7501 atagggtagg ggagaggcag atgcgaatta aaccagacga taactggcgt tggtattacg
     7561 atgaagagca cgatcgaatg atgctcgatt tagccaatgg tatgctattt cgctcacgtt
     7621 ttgcgcgcaa gatgttgact ccagatgcct tttctcccgc aggcttttgc gttgacgacg
     7681 ccgcgctcta tttctctttt gaagaaaagt gccgcgattt taatttatct aaagaacaaa
     7741 aagccgaact ggtgctgaat gcactggtag caattcgcta tctcaaaccg caaatgccga
     7801 aaagctggca ttttgtttcc catggtgaaa tgtgggtgcc catgccgggc gatgcggcct
     7861 gcgtttggtt gagcgatact cacgagcagg tcaatttgct ggtcgttgaa tctggcgaaa
     7921 atgccgcact atgcctgctg gcacaaccct gcgttgttat tgcgggtcgc gcgatgcagt
     7981 tgggcgacgc cattaaaatc atgaacgaca ggctgaaacc gcaggttaat gttgacagct
     8041 tcagcctcga acaggcagtc taacgcgcca acttaagtgc agtcttcggt acacagctac
     8101 agcaaagaat ggtgccatca tcgcccattg ctgatttttt cagcggcgtg acttcgcctt
     8161 ctaaaagctg aacacggcaa cttccacaaa tgcccgcgcg gcaagaataa gggatacgaa
     8221 ttccctgatt ttctaattgc tccagcaaca cctgttggtt atttccacga aatgcctgtc
     8281 cctgccagtc aatatctaca tttgcgtccg gttgttgcgt gatgttggcg gtatcatcag
     8341 cggcagctgc gccgtaaatt tttgccggag ccgttgccag aatttccacc tcatcgccaa
     8401 cgcgaatcac gccgctatta cgggcaatta aattctgacc aaaatcgaca tcgccgttat
     8461 cctgggcagt gcggaaagat tgcaatgttt ttaatggttc gcctgccgga tgtttttgcc
     8521 ctttttctgg gctgacggtg gtgaaaatac agcggctaca aggtttaacc acatcaaaca
     8581 ccacatcacc aatgcgaatc actttccagc gatcttcttc ccaggctgac gcgccggaaa
     8641 ccaccagatt ggggcggaat tgctccattt ttacactggc cggacaacgt tgttggagat
     8701 cacgtaacga ggcttcgtta gcaagaaggt aaggatagcc atcagcaaat gacagaggta
     8761 cagtgttgtg gcgtttcacg cgccgggtca tttgtggccc cacccagcgt aattgcactt
     8821 cgcgggagaa aaatccactt agccatttgt tgatcgcgtc tggcgcaatt cgcgcggtaa
     8881 aatgtgtgcc ccaaacttcg gttggtgcgt cttgtgtggc gaaatcagca aaacgaacat
     8941 atgcgctact gccatctggt gcggtgagat gcaagccatc atgcacaggt gaaggagtaa
     9001 accgtaccat ctggggaaac tggcgagcgg taataaacgt accgtcaggt tccgtgatca
     9061 taaagatgcg atcgaaggcc agaccactga catcagccag agcatgtgta agaccaatgc
     9121 cgcgcatcga tttaacagga tgaataaaaa gccgggttaa tgtcgccacg gtgcggtcct
     9181 caaatgaaaa taagccctca actttatgac atgacgcgct tattagctat aatgcgcaac
     9241 aattttctta gcgcctgtcc cattaggcta ttttatttgt cagtttggcc ctgggttgtg
     9301 ctcgaaatcc tcacgtactt gtgtacgctc cggtttctcc gcgctgtcca tgtccagact
     9361 gactgcaaca attacgccta ctgcggtagg ctcttagagt aaaagtgacg atatgaattc
     9421 tctgtttgcc agtacggccc gtgggctgga agagctgtta aaaactgaac tggaaaacct
     9481 gggggccgtt gaatgccagg tggttcaggg tggggtccat ttcaagggcg acacacggct
     9541 tgtttaccag agcctgatgt ggagccgcct ggcctcgcgt attatgttgc cgctgggcga
     9601 gtgtaaggtt tacagcgatt tagacctcta tctcggtgtt caggcgatca actggacaga
     9661 gatgtttaat cttggcgcga ccttcgctgt ccacttcagt ggtttgaatg acaccatccg
     9721 caacagtcag tacggcgcga tgaaagtgaa agacgcgatc gtcgatgctt tcacgcggaa
     9781 aaatctgccg cgtccaaatg ttgatcgcga tgcgccggat atccgcgtta acgtctggct
     9841 gcataaagaa accgccagta tcgctcttga tctgagtggt gatggtttac atctgcgtgg
     9901 ctatcgcgat cgtgctggta ttgcgccgat caaagaaacc ctggcagccg ctattgtgat
     9961 gcgaaccggc tggcagccag gaacaccgct gctcgatccg atgtgtggtt ccggtacgtt
    10021 gctgattgaa gcggcgatgc tagcgaccga tcgcgcacca ggcttgcacc gtgggcgttg
    10081 gggctttagc ggctgggtgc agcatgatga agctatctgg caggaagtga aagcggaagc
    10141 gcaaactcgt gcccgtaaag gcctggctga gtatagctct catttttacg gttcggacag
    10201 cgacgcacgg gtgattcaac gtgcgcgcac taacgcccgt cttgcgggga ttggtgaact
    10261 gatcaccttt gaggtgaaag atgtcgcgca actgaccaat ccgctgccga aagggccgta
    10321 cggcacagtg ttgagcaacc cgccatacgg ggaacgtctg gacagcgaac cggcgctgat
    10381 tgcgctgcat agcctgctgg gtcggatcat gaaaaaccag tttggtggct ggaatctctc
    10441 tttgtttagt gcctcgccgg atctgctaag ctgcttgcag ctgcgtgcag acaaacagta
    10501 caaggcgaaa aacggcccgc tggactgcgt acagaaaaat taccacgttg ccgaaagcac
    10561 tccagacagc aaaccggcga tggtagcgga agactacgcc aaccgcctgc gtaagaacct
    10621 caaaaaattc gagaagtggg ctcgccagga aggaattgaa tgttaccgcc tgtatgacgc
    10681 cgatctaccg gaatataacg ttgccgttga ccgttatgcc gactgggtgg tggtgcagga
    10741 gtatgcgccg ccaaaaacta ttgatgctca caaagcgcgt cagcgtctgt tcgatattat
    10801 cgctgcaacc atttcggtac tggggattgc gccaaacaaa ctggtgctga aaacccgtga
    10861 acgccagaag ggcaaaaacc agtaccagaa actgggcgag aagggcgagt ttcttgaagt
    10921 taccgaatat aacgctcact tgtgggtgaa cctgacggat tatctcgata ctggtctgtt
    10981 cctcgatcac cgtatcgccc gccgtatgct cggtcagatg agcaaaggca aagatttcct
    11041 caacctgttc tcttataccg gtagcgccac cgtgcatgcg ggattaggcg gcgcgcgcag
    11101 caccaccacc gtggatatgt cgcgtactta tctggagtgg gcagaacgca acctgcgtct
    11161 gaatggcctg accgggcgtg cgcatcgcct gattcaggcc gattgcctgg cgtggctgcg
    11221 tgaggcaaat gaacagtttg atctgatctt tatcgatccg ccaaccttct ctaactcaaa
    11281 acgaatggaa gatgcgtttg atgttcagcg cgatcatatg gcgctgatga aagatttgaa
    11341 acgtctgctg cgtgcaggtg ggacgatcat gttctcgaac aacaaacgtg gcttccgtat
    11401 ggatctcgac ggcctggcaa aactgggact gaaagcacaa gaaattacgc aaaaaacgct
    11461 ctcccaggat ttcgcccgta accgccagat ccacaactgc tggctgatta ccgcagcctg
    11521 aaaggaatag taatgtcatt aatcagtatg catggcgcat ggctgtcgtt cagcgacgcg
    11581 ccgcttctcg ataacgcaga actgcatatc gaagataacg aacgtgtttg tctggtgggt
    11641 cgcaacggcg cgggcaaatc gacgttaatg aaaatcctca accgtgaaca agggctggat
    11701 gacggtcgca ttatttacga gcaagatttg attgtagctc gtctgcaaca ggatccgccg
    11761 cgtaacgttg agggtagcgt ttatgatttc gttgccgaag gcattgaaga acaagcggaa
    11821 tatctgaaac gctatcacga tatttcgcgc ctggtgatga acgacccgag cgagaaaaat
    11881 ctcaacgaac tggcgaaggt tcaggaacag ttggatcacc acaacctgtg gcagctggaa
    11941 aaccgcatca acgaagtgct ggcgcaactg gggttagatc ctaatgttgc gctgtcgtcg
    12001 ctttccggcg gctggttgcg taaagcggca ttaggacgcg cgctggtgag taatccgcgc
    12061 gtgctgttgc ttgatgaacc gacaaaccac ctggatattg aaaccatcga ctggctggaa
    12121 gggttcctta aaaccttcaa tgggacgatt attttcatct cccacgaccg ttcgtttatc
    12181 cgcaatatgg cgacgcgcat tgttgatctc gatcgcggca agctggtgac ctatccaggg
    12241 aattacgacc agtacctgct tgaaaaagaa gaagccctgc gcgtggaaga actacaaaat
    12301 gccgagttcg atcgcaaact ggcgcaggaa gaggtgtgga tccgccaggg gatcaaagca
    12361 cgccgtaccc gtaatgaagg ccgcgtacgc gccctgaaag caatgcgtcg cgagcgtggt
    12421 gaacgtcgcg aagtgatggg taccgcaaag atgcaggtgg aagaggccag ccgctccggt
    12481 aagatcgttt tcgaaatgga agacgtttgc taccaggttg acggtaagca actggtgaaa
    12541 gatttttcgg cccaggttct acgtggcgac aaaattgccc tgattggtcc gaatgggtgc
    12601 ggcaaaacca cgctgctgaa actgatgctc ggtcagcttc aagcggacag cgggcgtatt
    12661 cacgttggca ccaaactgga agtggcttat ttcgatcagc accgcgcgga actggatccc
    12721 gataaaacgg tgatggataa ccttgccgaa ggtaagcaag aggtgatggt taacggcaag
    12781 ccacgccacg tattgggcta tttgcaggac tttctgttcc atccgaaacg ggcgatgacg
    12841 ccggtacgtg cgctttctgg cggtgagcgg aaccgcttgc tgctggcgcg tttgttcctc
    12901 aaaccaagca acttattgat tcttgacgaa ccgaccaacg atcttgatgt cgaaacgctg
    12961 gaactgctgg aagaactgat cgacagctat cagggcacgg tattgctggt tagccacgat
    13021 cgtcagtttg tcgataacac cgttacagaa tgttggatct tcgaaggcgg cggtaaaatt
    13081 ggtcgttatg tcggcggtta tcatgatgcc cgtggtcagc aagagcagta tgtggcgctc
    13141 aaacagcctg cggtgaaaaa aaccgaagaa gccgccgcgg caaaagcaga aactgtaaaa
    13201 cgcagcagta gcaaactaag ctataaattg cagcgcgaac tggagcagct accgcaattg
    13261 ctcgaagatc tggaggcgaa gctggaagcc ctacagacgc aggtggcgga tgcttccttc
    13321 ttcagtcagc cacatgagca gacacaaaaa gtgctcgccg atatggctgc tgcagagcag
    13381 gagctggagc aagcctttga acgctgggag tatcttgaag cgttaaaaaa tggtggctga
    13441 tcgcaaaata gtcgatttaa acagagacag ctgacaacgg taaaattgcc tgatgaactg
    13501 ggtttatcag gcatgaaaac caacactttg ttagtcatct gtaaaatagc gcatcattaa
    13561 ggagtaccaa tgtgcgaaca tcatcatgcc gcgaagcaca tcctgtgctc gcagtgtgac
    13621 atgctggtgg cgttaccgcg ccttgagcat ggtcagaaag cggcatgtcc ccggtgtggc
    13681 acaacgttaa ccgtggcgtg ggatgcccct cggcagcgtc cgaccgccta tgcgttggct
    13741 gcactgttca tgctgttgct gtccaacttg tttccttttg tgaatatgaa cgttgcagga
    13801 gttaccagtg aaattacatt actggaaatt cccggcgtgc ttttttctga ggactacgcc
    13861 agcctcggca cctttttcct gttgtttgtg caactggttc ccgcgttttg tctgataacc
    13921 attctgttac tggtgaatcg cgcggaatta ccggtccgtt taaaagagca actggcacgg
    13981 gtgctttttc aactcaaaac ctggggaatg gcggagattt tcctcgctgg tgtgctggtc
    14041 agtttcgtta aactgatggc ttacggcagc attggggtag gcagcagctt tctcccctgg
    14101 tgtttatttt gtgtcctgca actgcgcgct tttcagtgcg ttgatcgtcg ctggttatgg
    14161 gacgacatcg ccccgatgcc agaactgcgc cagccgctaa aaccaggcgt cacggggata
    14221 cgtcaggggc tgcgttcgtg ctcctgttgt acggcaatcc ttcctgctga tgaacccgtg
    14281 tgcccgcgtt gtagtaccaa agggtacgtt cggcgtagaa acagcctgca gtggacactc
    14341 gcgctgcttg taacgtccat catgctgtat cttccggcta atattttgcc catcatggtg
    14401 acggatttat taggctcgaa gatgccgtcg acgattctcg ctggggtcat tctgttatgg
    14461 agcgaaggct cttatcccgt cgctgcggtg atctttctgg ccagtattat ggtgccaacg
    14521 ttaaagatga tcgccatcgc gtggctgtgt tgggatgcca aagggcatgg caagcgcgac
    14581 agtgaaagaa tgcatttgat ttatgaagtt gttgagtttg taggccgctg gtcgatgatt
    14641 gacgttttcg ttatcgcggt gctctcggcg ctggtgcgta tgggaggttt aatgagtatt
    14701 tatccggcaa tgggtgcatt aatgtttgct ttagtcgtca taatgacaat gttttctgct
    14761 atgacgtttg acccgcgttt gtcgtgggat cgtcaacctg aatcagagca tgaggagtcc
    14821 tgacagtatg gaatctaata atggggaagc caaaatccag aaagtgaaga actggtctcc
    14881 cgtgtggata tttcctatcg tcacggcgct cattggggcc tgggttcttt tttatcatta
    14941 cagccatcag ggactggaag tgaccctgat caccgcgaat gcggaaggaa ttgaaggtgg
    15001 caaaaccacc attaaaagcc gtagcgttga cgtcggcgtg gttgaaagcg ccacactggc
    15061 tgatgatttg acgcacgttg aaatcaaagt gcggctgaat tctggtatgg aaaaattgct
    15121 gcataaagac accgtctttt gggtggtgaa accgcagatt ggtcgcgaag ggattagcgg
    15181 cctgggaacg ctgctgtctg gagtttatat cgaactgcag ccaggcgcga aaggcagcaa
    15241 aatggataaa tacgatttgc tggactcgcc accgttggcc ccgcctgatg cgaaaggtat
    15301 ccgtgtgatt ctcgatagca aaaaagccgg gcagctctcg ccaggagatc cggtgctgtt
    15361 ccgtggctat cgggtaggtt cggttgaaac cagcaccttc gatacgcaaa aacgcaatat
    15421 cagttatcaa ctgttcatca atgcacctta tgaccgactg gtgaccagca atgttcgctt
    15481 ctggaaagat agtggcattg cggttgatct gacgtcagcg gggatgcgtg tggagatggg
    15541 ctcattgaca acgctgctta gtggcggtgt tagctttgat gtgccggaag gtctggattt
    15601 agggcagcca gtggcaccga aaacagcttt cgttttgtat gatgatcaga agagcattca
    15661 ggattcgttg tacaccgatc acattgatta tctgatgttc tttaaagatt cggtacgcgg
    15721 tctgcaaccg ggagctccgg tagaattccg gggtattcgc ctgggtaccg taagcaaagt
    15781 gccattcttt gcgccgaata tgcgtcagac atttaacgat gattaccgta ttccggtact
    15841 gattcgtatc gagccagagc ggctgaaaat gcagcttggc gaaaatgcgg atgttgttga
    15901 gcaccttggc gaattgttga aacgtggttt acgcggatcg ctgaaaaccg gaaacctggt
    15961 cactggtgca ctgtatgttg atcttgattt ctatccaaat acgcctgcaa taaccggtat
    16021 tcgtgaattt aatggttatc agattatccc gactgttagc ggcggcctgg cgcaaatcca
    16081 gcaacgactg atggaagcgt tggataagat caacaaactg ccattgaatc cgatgattga
    16141 acaggcaacc agtacgcttt ctgaaagtca gcgcacaatg aaaaacctgc aaacgacgct
    16201 ggatagcatg aacaagatcc tcgccagcca gtcgatgcag cagttgccga cggatatgca
    16261 gtcaacgttg cgtgaattga atcgcagcat gcagggcttc cagcctggct ccgcagccta
    16321 caacaagatg gtggcggata tgcagcgcct tgatcaggtg ttgcgagaac tgcaaccggt
    16381 gctgaaaacg ctcaatgaga agagtaacgc gctggtattt gaagcgaagg acaaaaaaga
    16441 tccagagccg aagagggcga aacaatgaaa aagtggctag tgacgattgc agcactgtgg
    16501 ctggccggat gcagctccgg cgaaattaat aaaaactatt accagttacc tgtggtgcag
    16561 agcggtacac aaagtaccgc cagccagggc aatcgtctgt tatgggtaga gcaggtcact
    16621 gttcctgact atctggcggg gaatggtgtg gtttatcaaa ccagtgatgt gaagtatgtg
    16681 attgccaaca acaacttgtg ggccagcccg ttggatcaac aattgcgcaa caccctggtt
    16741 gccaacctga gtacgcaact gcccggctgg gtggttgcct cccagcctct gggaagcgcc
    16801 caggacacgc tcaatgttac cgtaacggag tttaacggtc gctatgatgg caaggtcatt
    16861 gtcagtggtg agtggctgtt gaaccaccag ggacaactga tcaaacgtcc gttccgtctg
    16921 gaaggagtgc aaactcagga tggttacgat gagatggtta aagtactggc cagtgtctgg
    16981 agtcaggaag ccgcttctat tgcgcaagag ataaagcgtc taccttaatt ataaagattt
    17041 gtaaatataa ccgtctccgg tatgttgcct gaggcggttt ttttgtttct aacgtgcgga
    17101 aaaagttgtt cctctttaca ttttttgtac aaccgacata cccgtgtagc tcacaaatat
    17161 gacagtggcg tgaattttgc gcattgacgg cagttatgat tcgcggtatt gcttaactgt
    17221 gattgcacat ttagtaatca ctgttttctt ttccaccaga aaccagtatg agggaaacga
    17281 ggcatgaaga gacaaaaacg agatcgcctg gaacgggcac atcaacgtgg ttatcaggcc
    17341 ggcatcgccg gacgctcaaa agaaatgtgt ccctatcaga cgctgaatca aaggtcacaa
    17401 tggctgggag gctggcgaga agccatggcg gacagggtag taatggcctg attctgtctc
    17461 tttaaaaaga aacctccgca ttgcggaggt ttcgcctttt gatactctgt ctgattataa
    17521 tcagaaggca gaagtatcct ggaacagacc gactttcagg tcgctggcgg tatagatcaa
    17581 acgaccatca accagcactt ctccatccgc caggcccata atcagacgac ggttaacaat
    17641 gcgtttaaag tgaatacggt aggtcacttt tttcgctgtc ggcagtacct gaccagtgaa
    17701 tttcacttcg ccaacgccca gtgcgcggcc tttaccttcg ccgcccagcc agccgaggta
    17761 gaaccctacc agctgccaca ttgcgtccag gcccaggcat cccggcataa ccggatcgcc
    17821 aataaagtgg catccgaaga accacagatc cggattgata tccagttctg cttcaacata
    17881 ccctttgtcg aagttaccac ccgtttcggt cattttgacc acacggtcca tcatcagcat
    17941 attcggtgct ggcaattgcg ggcctttagc gccaaacagt tcaccgcgac cagaggcaag
    18001 aaggtcttct tttgtatagg attcgcgttt atctaccatg ttctctgtaa gccttatttt
    18061 attgaagcac gcaggatagc taacacgtgt acgctgaaca agtccgatca gttcggaata
    18121 aaccagttca gccaacgtaa tggccatgga aaacggtgac gtccttcctg ttgcgatgct
    18181 tgcgcgatac gttcctggat ggtttgcatc agcgtcgttt ggccttcgcc atcccacacc
    18241 agatttaata ataacggcag tgcgtcagtc acatcgtcta ctgcccagat ggtgaatttg
    18301 ccttcttcta ccgctttcac tagttcactg tgaagactta aatggcgaac gttagctgtg
    18361 gggataatga caccttgttt cccggttaac tcacgttgct ggcaaatagc aaagaagcct
    18421 tcgattttct catttaaacc accgaccggc tgggcgcgac cgaactgatc gactgaacct
    18481 gtgatagcga tactctgatt caccggcaca tcggcgaggg cgcttatcag ggcgcagagt
    18541 tcagccatcg aggcactatc tccatcaact tcactgtatg actgctcaaa tgtcagcgat
    18601 gctgagaagg ggatctgttg ctcaagctgt agttccgaca tcaggaacgc ttgcatgatc
    18661 atcatccctt tcgcatggat attgccgcca agctccgctt tgcgttcgat gtcggtgaat
    18721 tcaccatcgc caatatgcac aacgcagcta atgcgagaag gttcgccaaa agcgcgtgga
    18781 tgacccggaa attcaatgac cgaaagggcg ttaatttgcc cgatgcgttc gccttcggtt
    18841 tcaatcagga tttgctcctg aaggatctca tcctgcatac gttcagcgag gaaaccttcg
    18901 cgccattcac gctgctgcag cattaagttt agctgctcgc cggagaaggt gtcgccatca
    18961 cacagggagg cgacctcttt acactggcgg aggatccact gcgggctaag cggaagtgtt
    19021 tcttgttcac cggtgtagcg tgctgcttcg cggataagta ccggccaggc atccgctccc
    19081 ggtgcaggta agtgattatg tctggcggta aatgtcaccc agcgacacca ctgggttact
    19141 gactccgcat cgacaatctg cagagtatct tcaaattcgc tataaatagc ctgctctgaa
    19201 agctctggct ccatctcctg gaaatcagcc aatgattcgc gttcgcctac cagaatgact
    19261 ttcagcttca atggcatcga aggcacagag acggggagag ggcgcgactc atcaaacgca
    19321 acccagtcaa aacgctcgcg gttaacgata tttttcagcc gcatccacag cagaggttgc
    19381 gccagcagtg tacgcaaaga gatgatgaga ataccgccat ttgcctgatg caccagacca
    19441 ggctgcaggg taatgtcgcc attaaactgg cgcaggcagc caaagagttg ctccgcttct
    19501 acccagtcgg cagcgacaac ttgcgttaaa gtcgcaaaat tatcatctgc actcactgcg
    19561 tgacgtaagc ggatggagtg gccggaaacc tcatagtgac cgcccaccag ttggcctgca
    19621 tcgctttgta gcgtacgcgc ggcattggca ataagattca gatactcaga ctcttccggg
    19681 gccttcgcca gcataaaaga ggaggatgct cgcgtatgca gcaactgctc cagcgcaaat
    19741 tgcagccgcg gttgagtatc actgaataaa ggatcgtttt cgtcaatcaa atgtggctga
    19801 gcaaatattt cctgatagct atcggtatca ggaaccaggt cacgccatgc aagtttcgta
    19861 atggtcaaag ttgatgtttt ttagtctgtt gtcaaagccg cgattatacc gtaaccggca
    19921 ctacagcaca cgtagaaagc accgacaata ctccaggcat gggcgttaaa gctcacagga
    19981 tggagattct tttcttcact ggcctaaaaa gctgatattc tgtaaagagt tacacggtaa
    20041 cattgagatc gctatgaaat atcaacaact tgaaaatctt gaaagcggtt ggaaatggaa
    20101 gtatctggtg aagaagcatc gcgaagggga gttaatcacc cgttacatag aagccagtgc
    20161 cgcccaggaa gccgtcgatg tgttgctctc gctggaaaat gaacccgtgc tggtaaatgg
    20221 ctggattgac aagcatatga atccggaact ggtcaatcgc atgaagcaaa ccattcgggc
    20281 aagacgaaag cgccatttta atgcggaaca ccagcatacg cgcaaaaaat cgatcgatct
    20341 ggaatttatt gtctggcaac gtctggctgg tcttgcgcag cgtcgcggta aaacgctttc
    20401 tgaaacgatt gttcagctga ttgaagatgc ggaaaacaaa gagaaatacg cgaataaaat
    20461 gtcttctctg aagcaggatc tgcaggcatt gctgggtaag gaataactga cgaaagtcag
    20521 ttcaatttac taaaggcaaa aaaaaccccg cagcagcggg gtttttctac cagacgagaa
    20581 cttaagcctg cggctgagtt acaacgtctt tgataccttt aacttcgatc tctacgcgac
    20641 gatccggagc caggcagtcg atcagtgcag cacgctgttt cacgttgtca caggtgttgc
    20701 cagtaaccgg gttggattcg cccataccac gtgcggagat cttgtctgcc gggatacctt
    20761 tggagatcag gtaatcaaca acagactgag cacggcgctc ggacagaccc tggttgtaag
    20821 cgtcagaacc gatgcggtcg gtgtaaccca gaacaactac ggaaccgtct ttcggatcca
    20881 ggttgctcag ctggctgtac agctgatcca gagcagcctg accttccggt ttcagggttg
    20941 ctttgttgaa gttgaacaga acgtcagact tcagagtgaa gtgcttggtc tgtacttccg
    21001 gtgccggagc tggagccgga gcaactactg gagctgcttc gccctgaccg aaacggtagg
    21061 aaacccccag gctcagcatg ccgttgtccg gacgagtgcc gatggtgtgt gcgtcaccga
    21121 tgttgttggt ccactggtat tccagacggg tagcgatttc aggagtgatc gcgtactcaa
    21181 caccgccagc gaagaccgga gaaacgccgg tgtcgtggtt tttaccataa acgttggatt
    21241 tagtgtctgc acgccatacc ataccaccca gacgagtgta gatgtccagg tcgtcagtga
    21301 ttgggtaacc cagtttagcg gtcagttgaa cgccctgagc tttgtatgca ccgttttcaa
    21361 cgctgccttt gtacggcata cgacctaacc agtcgtaacc catttcaaag ccaacatacg
    21421 ggttaacctg gtaaccacca aaagcaccag cgcccagttg gttttcatgg gtcgggccat
    21481 tgttgttgat gaaaccagtg tcatggtact gggaccagcc cagtttagca ccagtgtacc
    21541 aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa accagccagt gccactgcaa
    21601 tcgcgatagc tgtctttttc attttttgcg cctcgttatc atccaaaata cgccatgaat
    21661 atctccaacg agataacacg gttaaatcct tcaccggggg atctgctcaa ttaactctac
    21721 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    21781 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta
    21841 atcttttttg tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct
    21901 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    21961 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    22021 tcgtggcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    22081 ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga
    22141 tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag
    22201 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    22261 cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt
    22321 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    22381 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    22441 cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc
    22501 ctgtgagtta ctgtatggat gtacagtaca tccagtgaca acaaagatca accctatttt
    22561 cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa acataaatta atcttgcccc
    22621 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    22681 tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    22741 caagaatacc tggcaacgtt gggcacaatt gaataccgat cattgtttgg cagttacagc
    22801 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    22861 tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag
    22921 tgtggccgat ccgttaccct caattactat cgggttgatg aaagtctatg gcgaaatcaa
    22981 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    23041 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    23101 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    23161 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    23221 ggtgccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    23281 gaatgggctg actctcttac gccgaaacag gagtttcctg cggaacttga gtaatctggc
    23341 gttgcagacg accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca
    23401 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta
    23461 atgcctcatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
    23521 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    23581 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    23641 cgcgaatttg cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct
    23701 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    23761 cctgatggta ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt
    23821 cgagcatgcg cggcagattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc
    23881 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt
    23941 cacccagtaa gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt
    24001 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc
    24061 cttccagtga tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac
    24121 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    24181 aacttgtcag caagatccaa tacccgtgat gcattccggt tatctgaatg atggcgtagc
    24241 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    24301 agtgacggct aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct
    24361 cattttcgtc attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt
    24421 gggcatcaat ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg
    24481 gtgcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    24541 aatgcggatc atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg
    24601 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    24661 gttcacgcaa tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg
    24721 cgacaaagta ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc
    24781 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag
    24841 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    24901 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    24961 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    25021 gcagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    25081 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    25141 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    25201 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    25261 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta
    25321 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    25381 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    25441 gacttagcat aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac
    25501 gcgcagcttg cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag
    25561 cggcaatttt aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga
    25621 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    25681 tcaataccgt accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga
    25741 catgaatagc ttcattgcca taaggcacca gagatagttt agtgatctcc cagcaggatc
    25801 gtgtcagcgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca
    25861 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    25921 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    25981 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    26041 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    26101 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    26161 caattactgg cgattcactg taagcgcggt ctggtatggg gcgagctgga atttgtactg
    26221 ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac
    26281 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt
    26341 gttttacgcc agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg
    26401 cgtgagcaaa cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg
    26461 ccggttaacc gactggaaga attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc
    26521 tggttgaaag atattgaaag cgctcggttg cagcataacc aggcgtatac cgaagccatg
    26581 cttaccgagt atgcggattt tttccgccag gtcgagtctt caccgctgaa tccggcgcag
    26641 gcccgggcag tcgttaatgg cgagcattct ctgttagtgc tggcaggtgc aggaagcgga
    26701 aaaacgtcgg tgctggtggc ccgtgcaggc tggttgctgg cgcgtggtga agcgtcccct
    26761 gagcaaattt tattgctggc gtttggtcgc aaagccgctg aagagatgga cgagcggatt
    26821 cgcgaacggc tacataccga agacattacc gcacgcacgt ttcatgcgct ggcgctgcat
    26881 attattcagc agggcagcaa aaaagttccg atagtcagca aactggaaaa tgataccgct
    26941 gcccgtcatg aactctttat tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag
    27001 gcgaagggct ggcggcaatg gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac
    27061 ttctgggatg atgaaaaatt acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt
    27121 ctgatgcgga tgcacggtgg tgcacaggca gaaatgattg ccagtgcacc cgaagagatt
    27181 cgcgatctgt tcagtaaacg tatcaagttg atggccccgt tattaaaagc ctggaaaggt
    27241 gcgctgaagg cagaaaacgc tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt
    27301 ctggagaaag gtcgctttat cagcccgtgg aagcatattc tggttgatga atttcaggat
    27361 atctcgccgc agcgggcagc gttgttagcg gcattacgca agcaaaacag tcagacgacg
    27421 ttgttcgctg ttggtgatga ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg
    27481 ctcaccaccg ctttccatga aaactttggt gaaggcgaac gttgtgattt agacacgact
    27541 taccgtttta acagtcgtat cggtaaggtg gcaaaccggt ttattcagca gaacccaggc
    27601 cagctgaaaa agccgctaaa cagcttaacc aatggagaca aaaaagccgt cacgttattg
    27661 gatgagagtc aacttgacgc tttgctggat aagctctctg gttatgccaa accggaagag
    27721 cgcattctga tcctggcgcg ttaccatcac atgaggcctg ccagcctgga aaaagcggca
    27781 acacgctggc cgaagttgca aatcgacttt atgaccattc atgccagcaa agggcaacag
    27841 gcggattacg tcatcatcgt tggcttgcag gagggaagtg atggttttcc ggctgcggcg
    27901 cgggagtcga ttatggaaga ggcgctactg ccaccggttg aggatttccc ggacgctgaa
    27961 gaacggcggt taatgtacgt ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt
    28021 aacaaagaga atccctctcc ctttgtggaa atactgaaaa atctggatgt gccggtggcg
    28081 agaaaaccgt aagaaacagg tggcgtttgc cacctgtgca atattacttc agacggtccg
    28141 cgagataacg ctgataatcg gggattagaa tatcgaccgc gtcgttgaaa tgcggcgact
    28201 ggattatgaa gtctgccgtt gccacgttgg tggcgaccgg aatgttccat accgtcgcca
    28261 gacgcagcaa ggctttcacg tcaggatcgt gcggcacggc atttagtgga tcccagaaga
    28321 aaatcaatac atcaattttc ccttctgaga tcaatgcgcc aacctgctgg tcacccccca
    28381 ttgggccact caacatcgcg ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg
    28441 tagtgcctgt tgcatacagt acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc
    28501 tcatcagcat ttgtttgcag tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta
    28561 aagtgcgagt cgtcagttcc ataatgtaca tccgtagtta actttcctac agattactgt
    28621 aagcacttat cgctgcaaga taaagaccga aaaagcctgc gcacaggcac aaaaatctca
    28681 ggaagatggt tgtttttccg cccactgcag gaaagtattt cgcgtttgtg ggtcagccag
    28741 tttaaaccaa tacttcagcc gttgttctgt gagcacctga gactgcggtg gaatagcatc
    28801 cagctcggaa acacccgaaa gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg
    28861 tagtgaagcg cgtttcgcgg atttgttata gcgttcaacc tcgacctcat aatcaatcgt
    28921 ttttgccgtt gaggtaatgg cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc
    28981 atccaacaat tcaaggcgcg gcgcggcatc aaaatggtta gcttcccgct cattctccag
    29041 gcgaggcagg cgaaaattga cctggttgat gagctgggta ttaaaactga ccaccagcgg
    29101 tggggagata tacagccgtt cttcactatt ggaaagatga atcgtcttct caacgcgaaa
    29161 cactaactga tgcggtccat tatccagttc aatgctgtcc gcaccgcgca gcagagagct
    29221 ggaaaccttc ttgccgtcga gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt
    29281 ggcaaaaacg gataccggca aacataatgc aattaaggtg gtcacgatgc cggttttcat
    29341 aggtagctcc tgtcaaaaga ccgcccgaat aataatgtgt caaaattttt ctttaatcat
    29401 acttactaac atatagacat atttcccgct tttgctctca ttcattcgta ttagctgcat
    29461 ggttggcatg tcggctttgt cgtacacttt gttaaacagt ctggaggaaa taatgaaaga
    29521 aaccgatatt gcaggcattt taacgtccac tcacaccatt gcgctggtgg gggcaagcga
    29581 caaacccgat cgtcccagct atcgcgtgat gaaatatcta ctcgaccagg ggtatcacgt
    29641 cattccggtt tcgccaaaag ttgccggcaa aacgctgctg ggccagcagg ggtatggcac
    29701 gctggcagac gttccggaaa aggtcgatat ggtcgatgtt ttccgtaatt cagaagcggc
    29761 gtggggcgtg gcacaggaag ctattgccat tggcgcgaaa accctctgga tgcaactggg
    29821 ggtgattaat gagcaagcgg cagtactggc acgggatgcc gggttgaatg tggtgatgga
    29881 tcgctgcccg gctattgaga tccctcgcct gggcctggcc aaataaaaaa tccccggaag
    29941 gcaaaaacct tccggggatt cgttcaggga tcagttacgc agacgcgggg cctggagttg
    30001 tttgcggatg gtctgcgcca gctcatccat ggatggctgt tcaggatgtt catcctgcaa
    30061 ttcgctactt agctgggctt cagccaggta ggtatgaacc ggtaggccgt tatcgtcctc
    30121 catcactacg tgataccacg gagcggcgcg aagctcgtca tttaccgcca gctcatcagg
    30181 cgacggttca gagagcgaat aaaccgggtc gatatccacg accactccga gataacctaa
    30241 cagggaatgg cggacctgct ggccgatacc gaatttgctg gcaatcatag tcacctcccg
    30301 ggaaatcttt acctttgata taagggtaaa attccacatt tcaagttaca tgacgcgaca
    30361 ggcaaaccct ttcagatata gcccttccgg ataggtagcg atcaccggat gatcggctgc
    30421 ctgacggaac tgctctataa attgtacatc acggccggca tcaattgcgg catccgcgat
    30481 gattttctga aataaatcgc tggtcatcag accggaacag gagaaagtca ggagaatacc
    30541 gccttcattc agcaactgaa tcgccagcat gttgatatct ttatagccac gacacgcgcc
    30601 catcaactgg cttttattct caacaaactt cggcgggtcc atcacgataa cgtcaaattt
    30661 ttcaccgcga tcgcgataag tacgcagcaa tttaaagaca tcatcacgga caaactcagc
    30721 cttgctcaga tccagtttgt tcagctcaac gttctgccgt gcaatatcca gcgcttccta
    30781 ggaggtatca acgctgacaa cctggctgca accgcccatc agtgccgata cggcgaaacc
    30841 accggtatag gagaaacagt tcagcacacg tttattttca acgtagcggc gggtagccag
    30901 gcggctgtct cgctggtcca ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag
    30961 cagcttcatg ccgtgttctt caatcggcag cagggcaggc ggcaactcgc cggtgacgag
    31021 gccctgggtc agctccatcc cttctttttt acgtaccgca acatcgctgc gatcgtaaat
    31081 cgcacattcc gggtacagcg tttgcagggc actaattaat gccgcgcgct gatattctgc
    31141 cccagcactc agcagttgca gcaccagaaa attaccgaaa cgatcgatag taatacccgg
    31201 caggccatca gattctccgg cgattaaacg atagctgtcg aggccatctt tttgcgccag
    31261 tcagtcacgc catttttgtg cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga
    31321 ctcagacggg tcaaacgtcc agacgcgcgc ccggatttgc gaagctggcg aataagcgcc
    31381 gcgtgctaac cattttccct gatgatcaac aatatcgatg gtttcaccga ggctggcttt
    31441 accttccatg cgggcaacgg ccccggaaaa gacccacgga tggcgacgaa gtaatgattt
    31501 ttcgcgccct ttggctaaca ctaaacgtac actcataatt tacttttctg tcgatgcaaa
    31561 gaaatgggcg tcattgtccg gagttttcca tggatttgca acgcgtccat taaggataag
    31621 gagagcagca atgtcgaaag tctgcataat tgcctgggtt tacgggcgag ttcagggcgt
    31681 aggatttcgc tacaccacac agtacgaagc gaaaagactg ggtttaaccg ggtacgccaa
    31741 aaatcttgac gatggcagcg tggaagtggt tgcctgcggt gaagaagggc aggtggaaaa
    31801 attaatgcag tggttaaaga gtggcggccc gcgttctgcg cgtgtagaac gggtgctcag
    31861 cgagccgcat catccctcgg gggaattaac cgattttcgt atccgttaaa tacattttac
    31921 cggttttggc aggccagcaa ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg
    31981 gtataaatag cggctgttac ctttttcttc gccaaattta ttcgccatcg cttttaccag
    32041 catacgaatc gccggagaag tattgaattc cagatagaaa tcacgcacaa aacgcaccac
    32101 ttcccagtgt tccggtgaca gcgaaatccc ttcgttctct gcaatcacca ctgccagcgg
    32161 ctcactccac tggctgcttt ctttgagata gccttcggta tccgtttcta tctctttacc
    32221 ttcgaagatc agcataatta ttactacctt aatcagactg gcggcagtgt aacaaaaaac
    32281 aaagccccgc ataaagcgag gctatgaaag tgttagcggg tgagattaat cgcggctggc
    32341 gaagcccaga atgctcagca ggctgacgaa gatgttgtac agcgaaacat acaggctaac
    32401 cgtggcacga atatagttcg tttcaccgcc atgaacgatg ttgctggttt caaacaaaat
    32461 agcgccagag gagatcagaa tgaagaccgc gctgatcgcc agatgcagag caggcagctg
    32521 caggaagata ttagcaacca taccaatcag caccaccaca atacccgcca tcagcatacc
    32581 gccgaggaac gacatatctt tgcgggtggt cagcacatat gcagagcagc agaagaacac
    32641 taacgccgtt ccgcccagtg ccatagcgat tacgtcaccc attccggcag acagataggt
    32701 gttcagaata ggtccgagga tataacccag aaaaccggta aaggcgaatg cggagataat
    32761 cccggtcggc ttattcgccg ttttataggt caggaacatc aaaccataca tacccaccag
    32821 cgtcagaatc agacccggag atggcagcat cagcaccgta ctggcagttg cggtaatcgc
    32881 cgaaaaggcc agcgtcaggc tcagcagaaa ataggtatta cgcagcacct tatgggtgct
    32941 aagcagtgat gtacggtcat gtgaagaact aacaatacga tccattgagt cactctctat
    33001 gacagatgta attaattaag cagcataatg ataatgcgta agggcaccca gaagttttac
    33061 ccatctttac gcatttgatc tggaacaggt ttaacagcgg attatcaggt cattaagcaa
    33121 atataacgcc ctgagaattt cgacaggcaa aagaaaaagg ggttagcatt tagctaaccc
    33181 cttatcttat ttggcggaag cgcagagatt cgaactctgg aaccctttcg ggtcgccggt
    33241 tttcaagacc ggtgccttca accgctcggc cacacttccg gaatgacgcg cactataaac
    33301 atcccgatgc ggcgtgtaaa cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg
    33361 cattgatagc gtgaaataac agcagattga tcatttcatc accatgaatt ccttctcttt
    33421 tactcgttta gcaaccggct aaacatcccc accgcccggc aaaaagaaaa ataggtccat
    33481 ttttatcgct aaaagataaa tccacacagt ttgtattgtt ttgtgcaaa
//
LOCUS       DAZPBM010000027        33506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPBM010000027 DAZPBM010000000
VERSION     DAZPBM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.027"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1014)
                     /gene="pilV"
                     /locus_tag="ACHUZQ_001193"
     CDS             complement(<1..1014)
                     /gene="pilV"
                     /locus_tag="ACHUZQ_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFR1024939.1"
                     /translation="MKKTDKGVSLLEVLLVIGIMVMVIPKVYENIENHLNNVRWQNAA
                     EHANTYNTAVRNYVADNASTLLAGSLPKTITPATLIQKGYLKSGFSESNFGQSYITGI
                     AKNSKTSRLEALTCSNGGQSLSEAGMRSVASMIEGLGGYINSSKQAIGAGGGWSDTPS
                     NYGLNCATGHIAMALVGADLQESDRLYRYSITNRPDLNRMHTAIDMNSNNLNNVGTLN
                     GNAAALSGDISARNGTFSGAISGNTATTNGDITSNNGWLVTKNSKGWMNSTYGGGWYM
                     SDSSWLRSVNNKGIYTGGQVKGGTVRADGRLYTGEYLQLEKTATAGASCSPNGLVGRD
                     STGA"
     gene            complement(1027..1662)
                     /locus_tag="ACHUZQ_001194"
     CDS             complement(1027..1662)
                     /locus_tag="ACHUZQ_001194"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFR1024940.1"
                     /translation="MLNSIFNTAILCCANIVCQCYAYNEVKFRLNKLNVSYKTGTEKY
                     YCLILTTLAVLYLSLNQLSLIQSIIVIVFYAFLTLMACIDLLSFLLPRLYTVTFIFSG
                     LLYQTWNNNILSGLFCAMLMFFIMLFVRLYFAYKNGTESFGMGDVLLIAGTGVWFPTP
                     EIACSIVFIAVIGGIIFFTLGGLNKQKKYIPFGPFLCGGMFVYSLVPGILF"
     gene            complement(1666..2148)
                     /locus_tag="ACHUZQ_001195"
     CDS             complement(1666..2148)
                     /locus_tag="ACHUZQ_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1024941.1"
                     /translation="MRSVLYILLSCISSLIFSLQPAYSATTTRFDACFAQAGQRYLID
                     PLLLKSIATVESSLIPDRINHNKEKGKILSTDYGLMQINSSHIPNLKKLGVIKDKSEL
                     IDNPCLNIQIGAWILATHFQKCGINWSCLGSYNAGFKESNEQKRIKYARYVYNKYMVR
                     "
     gene            complement(2215..2772)
                     /locus_tag="ACHUZQ_001196"
     CDS             complement(2215..2772)
                     /locus_tag="ACHUZQ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFR1024942.1"
                     /translation="MSSINILNMRSVFSSLSAHRKKEQDKGATLMEVLLVVGVIVVLA
                     ASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSLKFQGRYTDSNYIKTLYAQGLLPSDM
                     IADTTGASAKNPWGGSVTITTSSDKYSFNVVEASVPKKNCMAMVNALRSSSAISKINN
                     TSTSTVDAATVCSSDSNTLTFSTDS"
     gene            complement(2817..3926)
                     /locus_tag="ACHUZQ_001197"
     CDS             complement(2817..3926)
                     /locus_tag="ACHUZQ_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFR1024943.1"
                     /translation="MAIKDIFEKLNSIEIDFSGISHWLAKYTFRYSRISLYEDLSGML
                     NDGINVKRALTHLIDVETDYGKKTGSSATYYICCECLNALNNTGAISSALKKYIKEDE
                     YQLISSGEIRGDLAGGLVQAIKIVRSRSEMTMTLVMSMIYPSVLVFGCLANMYMVHDT
                     ITPVFLSLAPKERWTSSMRLLTDTSGFLIENGTYILCFCIVLFFLIRYSLARYTGPGR
                     QYLDYIPPWSLYKTFNGVSFLFNMASMLSIDIPVAKALERLEKNSTQNRWLLERIKEI
                     RRYVLLGQSLAMAMKSCGYDFPSKLCINKLLLHSENADNALMMSNYADKWLEEAKGKV
                     KSTGVWITVVSALIVFAFIVMMITALYDVSNVLQH"
     gene            complement(3917..5455)
                     /locus_tag="ACHUZQ_001198"
     CDS             complement(3917..5455)
                     /locus_tag="ACHUZQ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFR1024944.1"
                     /translation="MFFSDADYPGKDISDVVYVSISEKDDKPVQVYIADNSKGQRAVQ
                     AYIGTLNVKYPGQVNITSTTLDVIASRYQDSEKRSDSNSSQHLNSNQEKVISYLAKAN
                     KLGSSDLHITPGRDGSEFTYVEARVHGELEILDVIPRKEGLELLGAAYSGMSDVIKGT
                     QFDPAIPQDARIAENFLKPVNLFGARYSHYPCVGGVYAVFRLIKDDSEDIPTFEELGY
                     MPQQIQTIRRMLQRPEGIIVLSGPTGSGKSTTLRTASEAYLSTFGFNHNDNMRLPRKR
                     LFTIESPPEGRIPGAIQTAVRDSVDGWVDAIKSAMRLDPDAILNGEIRDHASALAAIK
                     ASMTGHLLLTTLHANDALNIIERLEMENIQARLIADPLLFIGLLSQRLVQKLCPYCKR
                     TYAEMADKLSTEERKLIEENCLPEQVRLRNLDGCEHCYKGVTGRTVIAEVVSPDARFF
                     QLYRQSGRLEAKSYWHYELGGITRNQHLLHLINHGLVDPLSAHFISPIDEDKYSMLPE
                     GTWR"
     gene            complement(5480..5974)
                     /gene="pilP"
                     /locus_tag="ACHUZQ_001199"
     CDS             complement(5480..5974)
                     /gene="pilP"
                     /locus_tag="ACHUZQ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFR1024945.1"
                     /translation="MLKIIKPTIISVCFFSSLAYATESESTPKDEIKYSSPTILQVEQ
                     IQAETVIYEAKLARQKAVSELRNLDLNGVASATPAITPSSGYSSLADTDIPAQRVAAK
                     SLRVIEIFGTPQQMSARISLLDGSVTDVKVGQSIPGTSYKVASITGNGVWIQNGTEKR
                     QLIL"
     gene            complement(5958..7280)
                     /gene="pilO2"
                     /locus_tag="ACHUZQ_001200"
     CDS             complement(5958..7280)
                     /gene="pilO2"
                     /locus_tag="ACHUZQ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFR1024946.1"
                     /translation="MEVTLSENNQNNRNFTSVIKNKRAFFSGLDWKTLPSEEKNARTF
                     ARKNDAEYFLSCQYQDSENETKTMVAFIRKEDLPTGASSFWSLALMIKPLIEPDGYAI
                     CELGDLYGFVSCVNNVLVNDVVGNKSQIMSALTTFLEFNETPDPGWKLYQPESWDISQ
                     ALPSLTLSALIDVKKPPKEAAFTRVSRKRQFMIYGGSAILAILLWNGITMYQEYREKE
                     AAAEAARIRLAKEMADKQAIQIAPPWQHLPEIKPFIDKCIDKWDALPLSIAGWRFDLA
                     ECSTSGNDGLLRTSYKELSGVTVEDFSTRIREIFQGTTTATFVLPEGSAGGFSLPVSF
                     DVSPDPITPDTLPQATDIQERLTTFAQKMRLKLTWQEIENTKTDEEGRPIILPWNEYE
                     LMIQTSTPPSILFANFHEPAVRFQYAGIKLEEGRLNYEIKGAFYVKNN"
     gene            complement(7319..8962)
                     /locus_tag="ACHUZQ_001201"
     CDS             complement(7319..8962)
                     /locus_tag="ACHUZQ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFR1024947.1"
                     /translation="MNKKLLCLLISSFLLSGCANFKKINANELAAERDAQTATEYAKK
                     IRNNPVIQDSTKQWINTTPIIEKQVKQSAPPCPIVINTRSDIELRQIAQRITQTCHIP
                     VRISADVWTYLNGTGTGSTQQMTGSIPAPDANGMVPLASVGSQPVKVSSGGTSLPELN
                     ISGLPALLQTVSSRLGISWKYDKGRITFFYLETRSFPITYMDSNVAYNSKVVSGTMSS
                     SGSTGSTSSGGMTGDASNTQTTTVEMKSSLYNDLKSEVSSMLTPGTGRMYLSTGSLTV
                     TDTPDVLDSVQEIVNKRNNEMSRQVVLNVEILSIKKSSQEQAGIDWNAVFNDGHLGLS
                     LGGSFGDAAENVITSGVSIVDGKLAGSKAFLKALSSQGSVSVVTQNSAVTKNLTPVPM
                     QIANQQGFIESVTTDSTANVGSSTSLNAATITTGFNMTLLPYIQPDSQNLQLLFSMSL
                     SDKPTFEVFESGGSKAQTPNVDLKTINQTIDLKSGQTVILSGFQQSNRKTSKQGVITP
                     SFFGLGGGVNSEDGDTILVVLITPNVLNSGSSAVIKNVN"
     gene            complement(9013..10971)
                     /locus_tag="ACHUZQ_001202"
     CDS             complement(9013..10971)
                     /locus_tag="ACHUZQ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFR1024948.1"
                     /translation="MDAKKTGGLILFLLLLLVGVLIASNYLGGYTALRYSSVDMSLLK
                     WDTFHSVISTFSGNPQYKKLVFMAWFGFSVPLIFFAIFMLIVVIGIMPKKVIYGDARL
                     ATDMDLSKSGFFPDKKSPYKHPPILIGKMFKGRYKKQFIYFAGQQFLILYAPTRSGKG
                     VGIVIPNCVNYPGSMVILDIKLENWFLSAGFRQKELGQECFLFAPAGYAETIDQAIKG
                     QIRSHRWNPLDCVSRSDLLRETDLAKIAAILIPASDDPIWSDSARNLFVGLGLYLLDK
                     ERFHLEQKAKGHNVPDVLVSISAILKTSVPDGGKDLAAWMGQEIENRSWISDKTKSFF
                     FKFMSAPDRTRGSIETNFSSPLSIFSNPITAEATNFSDFDIRDIRKKPMSIYLGLTPD
                     ALITHEKIVNLFFSLLVNENCRELPEHNPDLKYQCLILLDEFTSMGKSEVIERAVGFT
                     AGYNLRFMFILQNEGQGQKSDMYGQEGWTTFTENSAVVLYYPPKSKNALAKKISEEIG
                     VRDMKISKRSISSGGGKGGSSRTRNDDVIERPVLLPEEIVSLRDKKNKARNIAIREII
                     TSEFSRPFIANKIIWFEEPEFKRRVDIARNNHVDIPNLFTQEVMDEIAKIAEIYLPKA
                     GGKKVMVAGGNVITNPDLDNHDKTDVSE"
     gene            complement(10987..12042)
                     /gene="virB11"
                     /locus_tag="ACHUZQ_001203"
     CDS             complement(10987..12042)
                     /gene="virB11"
                     /locus_tag="ACHUZQ_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043684 - type IV secretion system
                     complex [Evidence IEA];
                     GO_process: GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HFR1024949.1"
                     /translation="MNNIRDTSNTSQPRDNSKTARRYLDMTGIQSVLNIEHVTEISVN
                     KPGTIWFEGKNGWESKDAPDATFDNLMTLAKTLTNLSKIKIPLSHDNPIASVVLPGGE
                     RGQIIIPPATENNSVVISIRKPSLTRFTIDDYVRTGRFDNVRIATKHEAILTERQRYL
                     YELSRRPDGQSKAQFLREAVKDRLNFLIVGGTGSGKTTIAKAIADIFPPERRYITVED
                     VPEMSLPLHPNHIRLFYKKNTVEAKEIIEACMRLKPDHIFLAELRGNEAWSYLEALNT
                     GHEGSISTIHANNTYASFSRLASIVKQSDVGMTVDMDLIMRTIKTSIDVILFFNHTRM
                     TELYYEPEEKNRFLSTM"
     gene            complement(12093..13232)
                     /locus_tag="ACHUZQ_001204"
     CDS             complement(12093..13232)
                     /locus_tag="ACHUZQ_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFR1024950.1"
                     /translation="MKNKDDEKNNDAGNRGIIEVKGKAAPKKILILIILLIAALFVII
                     ILFKVLSREQVVQQTPLEKSDETLVTNTNNGVSLTTMMKNIEEKEKLDAANRRKAQEE
                     QEQKQEDNAPSAPASQKADQTAVNVIANGTPQTASGDPNQPQPLPKSVRQLMGDTMVK
                     IDNQEPGEKNQERDDLQGSQYADGKVSPVLNRRYLLSAGTALSCVLKTKIVTSYPGIT
                     MCQLTRDVWSDNGEVLLARKGALLIGEQNKVMTQGVARVFVNWTTLKDENVNVRIGAL
                     GTDSLGASGLPAWVDNHFGQRFGGALLLSLLGDGLDILKNSTQQTGSNSNITYENTSD
                     ATKEMAKTTLDNTINIPPTAYINQGTVLSVIVPRNIDFSSVYELQ"
     gene            complement(13222..13923)
                     /locus_tag="ACHUZQ_001205"
     CDS             complement(13222..13923)
                     /locus_tag="ACHUZQ_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFR1024951.1"
                     /translation="MYGTPSERDGRIQTVDYNEQDVFNVRVKAGAQTTIKFGQDETIK
                     DVGIGDPEAWSVSVRDNTLFLRPKAEEPDTNVTVQTNKHIYPLYLISTTKQPTYILRF
                     NYPKPPSATVMTEKPFPCTDGGIINGHYQLKGDKSIFPYQVWDNGEFTCMRWTNKQEI
                     PVLYRVDADGNEHLVNGDRNKNTMVYYDVAENLRLRLGDQVADIRTSSIVNRPWNKKG
                     TSNGKTRVEKFSYEK"
     gene            complement(13989..14723)
                     /locus_tag="ACHUZQ_001206"
     CDS             complement(13989..14723)
                     /locus_tag="ACHUZQ_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virB8 family protein"
                     /protein_id="HFR1024952.1"
                     /translation="MENTNYYEHSKKEASKKKFEEKNEKKDYFKAIRDFERSEIEIIK
                     KKAKTFTILAIGEFVVICILGFAIASLAPLKTAVPFLVRVDNSTGYTDIAPQLSDAKE
                     SYQDVETKYFLSKYLINYEAYDWQTIQEQADTVKTMSSQKVFSAYDTMIRADSSPLNI
                     LKNNYKIKVQINSVILLRKDMAQVRFKKMVLDLSGKPAPGYRATEWISTISFDWDKDI
                     KTEKERLVNPLGLQVLSYQPDPEVIK"
     gene            complement(14889..17246)
                     /locus_tag="ACHUZQ_001207"
     CDS             complement(14889..17246)
                     /locus_tag="ACHUZQ_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFR1024953.1"
                     /translation="MHKIKLKNSDNMDVAREYPDYRFHITDSIIFTSDRKMLASLVVA
                     GIPFETENDNVLTNLFNSVKNFLIGLGKEGDLYLWTHLIKKRITIDGEWNFDNAFLSR
                     FSKKYLALFTSSAFYKSTWYLTFGIPYDDVDTGIERMNEMTQQAQKALQPFNASVLSV
                     HNTYISEVADYLSLLLNTEHNMIPLSSTPLSSSICDSEWYFGADVLELRNNESDSKKF
                     ATNYILKDFPIETTPGQWDFLLKQPYEFILTQSFIFESPTKTLKNIDSQLNKLQSAND
                     AAKTQQEELEAGKEAVAAGITLFGSLHCALTVFGDTPDQARSNGIKLSAEFITSGKGF
                     RFSRASLASPFVFFSHMPLNKRRPLDTRRTITNLACLMSFHNYSSGKKSGNPIGDGSA
                     IMPLKTVSDSIYWFNTHYSPPEKNVTGQKIAGHGMILGATGTGKTTFEAAASGFLQRF
                     DPLMFVVDFNRSTELFVRAYGGSYFTLQEGIYTGCNPWQLAAGPESPVWHRLLAFLKR
                     WTQVLARDNQGNPCSDEHGIELNAAVESIMRMPVEERRTALLLDIVSPELQTRLAKWC
                     DNGEYAWAVDSPRNTFNPLHHKKVGFDTTVVLDTKNGIHPACEPLLAVLFFYKEIMQR
                     GGNLMLSIIEEFWMPANFPMTQSMIKSALKAGRMKGEMMWLTSQSPEDAINCAIFAAL
                     VQQTATKILLPNPDAKWEGYKEIGLTEKEFEKLKELTKESRTMLIKQSGSSVFAKMDL
                     FGFDEFIPVLSGSETGLSIFDEIIAEKGDVAPDIWIPELLKRLNG"
     gene            complement(17252..17572)
                     /locus_tag="ACHUZQ_001208"
     CDS             complement(17252..17572)
                     /locus_tag="ACHUZQ_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HFR1024954.1"
                     /translation="MDKVNEHVSESFMTYNGFNRPALIAGIPLMLLLFTAFFAVLTGF
                     PAIFLWGIKGIIIPVICALFLFIVKLACENDSNALRVIRLNLMGLLLKIRHRDLIIGY
                     SSVR"
     gene            complement(17643..17933)
                     /locus_tag="ACHUZQ_001209"
     CDS             complement(17643..17933)
                     /locus_tag="ACHUZQ_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001708011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR1024955.1"
                     /translation="MKVKSTLQYFILAFFLSLTASVAYAGGLDTATNTMTELKTWAFS
                     FGGVCALCYLIYNVIMAFMEKKSWSDVGMALVYCSLAGGALYAGNWALELFK"
     gene            complement(17933..18517)
                     /locus_tag="ACHUZQ_001210"
     CDS             complement(17933..18517)
                     /locus_tag="ACHUZQ_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFR1024956.1"
                     /translation="MQLSAAVLAALISQCAPDVSPDTMNALIMTESGANPYVIANVSD
                     GTSKYFKDEKGAIEYAEKLTAENKRFSAGLTQIYSKNFPSLNLTNKTVFDPCTNIKAG
                     AAVLTDNYLRQKEGSSNQKILRALSLYYSGNESTGFIKEKKFNNTSYLERVIRNANNY
                     IVPSIREKTDESEINPPNDEEKTSPDWDVFGDFS"
     gene            complement(18538..18936)
                     /locus_tag="ACHUZQ_001211"
     CDS             complement(18538..18936)
                     /locus_tag="ACHUZQ_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024957.1"
                     /translation="MQDNDDVSELDIATREVLILRCNLIQLASEYIKINPLKNTRIYT
                     DLIHRSADIIKNIEQGNIQNKHNYLSQDVKHTLVLYDLYKSDFEQCPGAKSLIPVREK
                     IVSLKELLSGANELDSIVRRPRRKKRSSRI"
     gene            complement(19055..19492)
                     /gene="pilM"
                     /locus_tag="ACHUZQ_001212"
     CDS             complement(19055..19492)
                     /gene="pilM"
                     /locus_tag="ACHUZQ_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFR1024958.1"
                     /translation="MGWFFSLLLIFLSIGSYYLTDNNNQTIQRMKLLPAKQEAIEFVH
                     FANVINDYLYKYPDKRNSGGTLTSEQIGITPVYDIHHIIYGKRVYIWSADTEGLMSAL
                     QQQTKHSAMLGRVKNKKIVDNQGNDMGVPVPSSIPEEAIVLIN"
     gene            complement(19498..20733)
                     /locus_tag="ACHUZQ_001213"
     CDS             complement(19498..20733)
                     /locus_tag="ACHUZQ_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFR1024959.1"
                     /translation="MKKLIIIPTTLLLNACSHAPEPAQAKGGWYELNTTIEQVRADTY
                     YNGVILQQPVSQPNSTVQYSNDNYLKKEAINVTPAPRIAETVKPRSEQSATAPKNQGS
                     NNSFLKPTTNSITSSGEIKTAAKGATKQHSEQPALSPAKPISSNNHQTQTAQKTDQQT
                     KPRPEQPPLMSKSGFLKKNANNAVPATTLTGPSTPEKQIYFVRHDGKGSLQKALKEIV
                     PSDWTAEISPEVTKTFRRTISWQGNDQWPYVLDKMLRNYGLTVIKNTEKRHLLIDFQQ
                     QKGKTSQKAPVSPDKTPASPVRTPVSSDKLPQKSLTPVTVPQKQTEKLTWNLKKGSTL
                     RDGLTEWASSSPCGNSTWNLVWDTPVNYRIDAPLIFRGNFESALKDTLMLYLSADKPL
                     YAFRNIAQCVITIKDTQQE"
     gene            complement(20736..21035)
                     /locus_tag="ACHUZQ_001214"
     CDS             complement(20736..21035)
                     /locus_tag="ACHUZQ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFR1024960.1"
                     /translation="MKSIILSMVAASAIALSAPVYASDPCASVLCLYGKAIGSGGGSE
                     CKSAEKDFFNIIKKKKGSIRWSKTFDARKAFLNQCTTADPAAISKIMSKFGRSWG"
     gene            complement(21102..21737)
                     /locus_tag="ACHUZQ_001215"
     CDS             complement(21102..21737)
                     /locus_tag="ACHUZQ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024961.1"
                     /translation="MKSIILSMVAATALLSGCADSKTQPVPESPLTSNDHQITLKIDT
                     VRTDLPDSSPVCLNGMNIYESLSERHPGLFAKNQNVIFRSAFFDDGQAISSETIRKIM
                     QSTPCVESQTTSAGSWEPATELIPRQAISGDTTLAEKDRTAGFTMRIAPEIERGNFHL
                     LLTTTDPIRKQTVEQNILLKPDQPLIVSGFTGVNADSQSENRIVIITPHVR"
     gene            complement(21890..22144)
                     /locus_tag="ACHUZQ_001216"
     CDS             complement(21890..22144)
                     /locus_tag="ACHUZQ_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HFR1024962.1"
                     /translation="MNKSRNAIIASSIIFASLMLVGCKEKIYSVEYYSNNISEATKTL
                     EDCKKGTITDQNCDNARAALQQKQDSEYKKKVSEMRRRLD"
     gene            complement(22146..22787)
                     /locus_tag="ACHUZQ_001217"
     CDS             complement(22146..22787)
                     /locus_tag="ACHUZQ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR1024963.1"
                     /translation="MKDKVLSFLFAGIITITSPSIFASGIPVVDVAAIAKTVEEGLNR
                     AAEAARQLEQLKQQYEQTIRYAEEQKKRLEGFTDFSNGFDSASSYMKDSLSTITNSAK
                     SDLSSLRSQYDLSSNVAETQQKYDAILAKIKFYENFNNEMQERAKRLTTLQKEFASAD
                     TPQKKADLSNQLNTEKLTMELQLKQYDIAERQLENERKAQYETYVRQKRRDLS"
     gene            complement(22793..23788)
                     /locus_tag="ACHUZQ_001218"
     CDS             complement(22793..23788)
                     /locus_tag="ACHUZQ_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HFR1024964.1"
                     /translation="MNIVSEFLEKVTTIVESTAATNAAKVAQAISPTFFAAIALYVIY
                     LIYEITYAQRDVLMSEVTKNIGAFALVGAFTYSAPYYSQFVIPFVMHAGSDLSAAVTG
                     GSGTATSVDNLWNMLSVTLNDFKNNELDTLEWYSFTDQLYIYLIWGVGYVGGLLLIYY
                     TTVFLTLSTFMVGILLSAGILFICFSLFASTRNMFSAWVGSCLNYILLNLFYSISFSF
                     VISFVEQTVPSSGNITLTTVVYFFMVTVISIFLVEQVGTLCSTLTGGVGINGLTSAAN
                     GFGGKVASGFMRASGLRSFTNGFASKMPNPFFNAGSRSASALVQGLNRGSKLKAG"
     gene            complement(23792..24049)
                     /locus_tag="ACHUZQ_001219"
     CDS             complement(23792..24049)
                     /locus_tag="ACHUZQ_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024965.1"
                     /translation="MKKNDDLITLPSHNSHALDYIIKKAFTPRKTKTSTATHNVYSYN
                     KEESTLNFQKIGEAIFFGTDLVIKLKPNISASGELFICQKD"
     gene            complement(24046..24348)
                     /locus_tag="ACHUZQ_001220"
     CDS             complement(24046..24348)
                     /locus_tag="ACHUZQ_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024966.1"
                     /translation="MTQAKANLRKLRRARYVPPRTVKKNIANTERVVSKAITGCGVFR
                     DIPQVKINLIETRKILQIELALSIANLSEANPIEIRKKIKSIDRQLSSTSKIRTTL"
     gene            complement(24611..25267)
                     /locus_tag="ACHUZQ_001221"
     CDS             complement(24611..25267)
                     /locus_tag="ACHUZQ_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024967.1"
                     /translation="MRNANEGISLAAIVAKETTTGKWNGTGGGIGLGTGGIGLFIGGV
                     SGSKQEQTQRAKNFESPKEEEYSLLNVYGSIIKVLVFGVLFGLSARLFTFLSDTGSDL
                     AEPVTTTTATGKIQSILGGMAEMVSILGTVVPFLVIIIAMIWFLFFSSKREKEELTRV
                     EREAAKTKLRIDVYHRLRYVESDHVVFDPITGREVPAERACINKLIEALADESNNVRG
                     "
     gene            complement(25452..25895)
                     /locus_tag="ACHUZQ_001222"
     CDS             complement(25452..25895)
                     /locus_tag="ACHUZQ_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1024968.1"
                     /translation="MLWFTLFLLCIALEIITGTGWLLLISLGALSSAIVGFFLPISQE
                     ANICFFASISILASIIKFFYDKKHKKLDTLLVNTGHSRFKGKEFTLSDDIVNGKGQLL
                     IGDTFWPVETLHDENYPAGTRVIVTDMQGITLKVMTIEDKSESKE"
     gene            complement(25958..26911)
                     /locus_tag="ACHUZQ_001223"
     CDS             complement(25958..26911)
                     /locus_tag="ACHUZQ_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1024969.1"
                     /translation="MVTSIPLLLLILVALIFVKSAVKIVPQGNAWTVERFGKYTHTLS
                     PGLHFLIPFMDRIGQRINMMETVLDIPKQEVISKDNANVTIDAVCFVQVIDAAKAAYE
                     VDNLASAISNLVMTNIRTVVGGMNLDDMLSQRDSINSKLLTVVDYATDPWGIKVTRIE
                     IRDVKPPKELTEAMNAQMKAERTKRARILEAEGIRQSEILKAEGEKQSQILKAEGERQ
                     SAFLQSEARERQAEAEARATKLVSDAIAEGDVQSVNYFIAQKYTEALQAIGTASNSKL
                     VMMPLDSSSLVSSVAGISELLKNVSQDTPKVDMKQAQQYLS"
     gene            complement(26957..27151)
                     /locus_tag="ACHUZQ_001224"
     CDS             complement(26957..27151)
                     /locus_tag="ACHUZQ_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1024970.1"
                     /translation="MPKYQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAG
                     KCFSVDVHISDFICKEIHPQ"
     gene            complement(27144..27596)
                     /locus_tag="ACHUZQ_001225"
     CDS             complement(27144..27596)
                     /locus_tag="ACHUZQ_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFR1024971.1"
                     /translation="MARKLKINPVIPECVADLKGYPLYIIVAYWGLRNNKTLTTTDVS
                     RNFLITQQQSYDVLTYIYQEAQKQITATKRTLFNEHKRRISAFRILDIDKGILRTPKK
                     EIAYASYDNIPTVTMKSTRCEIQDSFTELRRWMCSRKTGDRVEVFLNA"
     gene            28728..29756
                     /gene="repA"
                     /locus_tag="ACHUZQ_001226"
     CDS             28728..29756
                     /gene="repA"
                     /locus_tag="ACHUZQ_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFR1024972.1"
                     /translation="MQLAVISKRHWSELSPEEQVRFWQDYEAGIESSFLVPQENKGGT
                     TKRRRGEHSTKPKCENPAWFRPDNYKALGGQLGHAYNRLVKKDPVTGQNTLRMHMSLH
                     PFYVLKRQSVGRKYKFRPEKQRLLDAIWVVLVSFCDRGLHTVGMSVSRLAEEISPKDS
                     KGDVIPETAVTVSRLSRLLAEQVSFGTLGTSEKTIWDRESRQRLPKYVWITETGWKML
                     GVDLVKLQEQQRKRLAESEIRLQLIKEGVIREGEEISVHSARKRWYAQRSLDAIKSRR
                     KKAAERKRANRLAKLPYDEQRNEIARFILKRMPPDEAYWCTKERLEQLVARDLRQLEL
                     ALTASPPH"
     gene            complement(29854..30225)
                     /locus_tag="ACHUZQ_001227"
     CDS             complement(29854..30225)
                     /locus_tag="ACHUZQ_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1024973.1"
                     /translation="MAEYLQRFFVGGLEGLCFSLGRSPYARREAVGGTFNILSGAALA
                     AASEGIFVIIHSCFKDKYFPCGEATQGIKEQGLRIYCGFDVLCLLLSEVIPLRVFVDN
                     RRRFYSDTLLRPSKNCQSGTK"
     gene            31107..31259
                     /locus_tag="ACHUZQ_001228"
                     /pseudo
     CDS             31107..31259
                     /locus_tag="ACHUZQ_001228"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            31204..31326
                     /locus_tag="ACHUZQ_001229"
     CDS             31204..31326
                     /locus_tag="ACHUZQ_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1024974.1"
                     /translation="MVCCTLLIFTYLTRNRLCEVRLKDGNREVTASLAYESNGK"
     gene            31476..32072
                     /locus_tag="ACHUZQ_001230"
     CDS             31476..32072
                     /locus_tag="ACHUZQ_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFR1024975.1"
                     /translation="MTAIIEASMQPKKTPVIVVKKRRVLVMPENPVVNEKPQEVQKSA
                     VNENKKVQKKDAVAEKTHKKQPQPWYLKKQITFPPKYPKEYFEKCFNKVRAVFPELWT
                     DEKKNLPLKSGILQDVEKYLADNPDVDLTIEEWNCAVQVMTFRWQYLQNCTVPGATRY
                     DLYGKPAGTVKKAHATYAQLVLDARKKASEKKQLKRKG"
     gene            complement(32090..32374)
                     /locus_tag="ACHUZQ_001231"
     CDS             complement(32090..32374)
                     /locus_tag="ACHUZQ_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFR1024976.1"
                     /translation="MALDLKTPVIPGQKTEQTNADTARFINEATRKKSGATKVTNVRL
                     GDVYEDILEREALRTGQTKTAIMKAALAAYEHGLDENGKNHWILQSARLK"
     gene            complement(32448..33113)
                     /locus_tag="ACHUZQ_001232"
     CDS             complement(32448..33113)
                     /locus_tag="ACHUZQ_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HFR1024977.1"
                     /translation="MGRALLVASDKGGVGKSTTATNLASYLVNNSRTVIILKTDKNND
                     ILSWNEKRKQNGLLPVPVYEEYGDVSEVIKRLKKICEVLIIDCPGHDSKEFRSALTVA
                     DILLTLVKPSSDFEAETLTHVTEKVRTAQQINPDLQPWVLFTRINTTKPRHRKAAIDL
                     DKLLRENPVWIQPLRTRISDLDIFEAACNEGAGVHDVRRASSLSTAKAQIELLAQELG
                     INP"
BASE COUNT         8929 a         7095 c         7316 g        10166 t
ORIGIN      
        1 cgcccctgta ctgtcgcggc ctacaagccc gttaggggag caggaagcgc cagccgttgc
       61 tgttttttca agctgtaaat actcgcctgt atagaggcgt ccatcagcgc gtacagttcc
      121 accttttacc tggccaccag tgtaaatccc tttattattc acgctgcgca accaggacga
      181 atcggacata taccagccgc caccataggt gctgttcatc cagcctttgc tgttttttgt
      241 gaccagccag ccattattcg aagtgatatc accgttggtt gtggctgtat ttcctgaaat
      301 tgcgccagag aacgtaccgt tacgtgcgct gatatcgccg gagagcgcgg cggcgttgcc
      361 gtttaaagtg ccgacgttgt tcagattgtt gctgttcata tcaatcgccg tatgcatcct
      421 gttaaggtcg ggacgattag ttatggagta acgatacaaa cggtcgcttt cctgcaaatc
      481 agcgccaaca agtgccattg caatatgccc ggtcgcgcag ttcagcccat agtttgaagg
      541 cgtgtcagac cagccgccac ccgcgccaat cgcctgcttg ctgctgttga tgtagccgcc
      601 aagcccttca atcatgcttg ctaccgagcg cattccggct tctgacaggg actgaccgcc
      661 attactacag gtcagggctt ccagacggga tgttttgctg ttctttgcaa taccggtaat
      721 gtagctttgt ccaaaattgg attcagaaaa accagatttc agatagcctt tctgaatgag
      781 tgttgctggc gtgattgttt tgggtaaaga gccagcaagt agtgttgatg cattgtcagc
      841 aacataatta cgaacagcgg tgttataggt atttgcgtgt tctgctgcat tctgccagcg
      901 aacattattc agatggtttt caatgttttc ataaacttta gggataacca tcaccattat
      961 gccgatgaca agtaatacct caaggagaga tacaccttta tctgtctttt tcattgctct
     1021 taatcctcag aataatattc ccggtacgag tgagtaaaca aacattccgc cacaaaggaa
     1081 cggaccgaat gggatatatt ttttttgctt gtttaatccg ccaagagtga agaaaataat
     1141 tccgccgata acggcaataa atacaattga acaggcaatc tcaggcgttg gaaaccatac
     1201 gccagttcct gcaataagta gcacatctcc catgccgaaa ctttccgttc cgtttttata
     1261 tgcaaagtaa agtcttacaa acagcataat gaagaacatc agcattgcac aaaacaaacc
     1321 agacaatata ttgttattcc atgtctgata taacaggccg gagaagataa atgtcacggt
     1381 ataaaggcgt ggtagtagaa atgaaagaag gtctatacag gccattaatg taaggaaagc
     1441 atagaaaaca atcactatta tggattgaat cagtgatagc tggtttaatg aaagatacag
     1501 aacggcaagc gttgtcagta tcagacagta atatttctct gtgccggttt tataagatac
     1561 attaagttta tttaaacgga attttacttc attgtaggcg tagcattgac agacgatatt
     1621 tgcacagcaa agtatcgccg tattaaatat gctgtttagc ataagttacc ttaccatgta
     1681 tttattgtag acatagcgtg cgtatttgat tcttttctgt tcgttacttt ctttgaaacc
     1741 agcgttataa ctgccaagac aactccagtt tatgccacat ttctgaaaat gtgttgccag
     1801 aatccacgcg ccaatttgta tattgagaca ggggttatca ataagctctg atttgtcttt
     1861 tattacgcca agttttttca ggttggggat gtgtgatgaa ttaatttgca tcagtccgta
     1921 atcggtgctt aatatttttc ctttttcttt gttgtgattt atccggtctg gtatgagtga
     1981 actttctaca gtggcaatgc ttttcagaag caggggatca atcagataac gttgcccggc
     2041 ttgtgcaaag caggcatcaa atcgggtcgt tgtcgctgaa tatgcgggtt gcaatgaaaa
     2101 gattaatgat gaaatacatg atagcaggat ataaagaaca gaacgcacaa tactcctcct
     2161 gtaaacatgg aaaaaataag cgcccgaagg cgcttatccg atgcacatga aaaatcagga
     2221 atcagtgctg aaggtcagcg tattgctgtc agatgagcaa acagttgccg catcgacagt
     2281 tgatgtggaa gtgttgttaa tcttggagat tgcgctggaa gaacgcaggg cgtttaccat
     2341 tgccatgcag tttttcttcg gtacgctggc ctcaacaaca ttgaaggagt atttatccga
     2401 agaggttgtg attgtaacgg agccgcccca cgggtttttc gcgcttgcgc cagttgtatc
     2461 tgcgatcata tcggatggca gtaatccttg cgcgtagaga gtcttaatat agttactgtc
     2521 agtataacgc ccctggaatt tcagtgattt catgttggca atcactgtca ggacattgtt
     2581 ttgttcattg gatgattgaa tgttggattg caccattgaa tagagtttat atgcacttgc
     2641 tgcaagtaca acaataacac caacaaccaa taacacttcc atcagtgttg cccctttgtc
     2701 ctgttctttt ttacggtgtg cagaaagaga agaaaaaaca gaacgcatat ttaaaatatt
     2761 aatagaagac ataataaata attccttatt tattgagagg ttaaaaacat tagggattag
     2821 tgttgcagga cgttactgac atcgtagaga gcagtaatca tcattacaat aaatgcgaaa
     2881 acaattaatg cggatactac agttatccag actcctgttg atttgacttt tccttttgct
     2941 tcctccagcc atttatcagc atagttagac atcatcaatg cattgtcagc attttcagaa
     3001 tgaagtaata gtttgttaat gcataatttc gatgggaagt cataaccgca acttttcatt
     3061 gccattgcaa gtgactgtcc aagtaaaaca tatctcctga tttcttttat tcgttcgagt
     3121 agccatctgt tttgagtgct gtttttctcc agcctttcca gtgcttttgc aacaggaata
     3181 tcaatactta acatggaggc catgttgaag agaaaactta caccattaaa tgttttataa
     3241 agtgaccagg ggggaatgta atcaagatac tgacggcccg gtcctgtata acgtgcaagc
     3301 gaatatctga tcaggaaaaa cagtacgatg cagaagcaga gaatatacgt tccgttttct
     3361 atcaggaaac cagacgtatc tgtcagtaat cgcatagaac ttgtccagcg ttctttcggt
     3421 gcgagagaaa ggaaaacagg tgttatggta tcgtggacca tatacatgtt tgccagacaa
     3481 ccaaaaacca gtacggatgg ataaatcatc gacataacaa gagtcattgt catttcagaa
     3541 cgacttctga caatttttat ggcctgaact aatcccccgg caaggtcgcc gcgaatttca
     3601 ccgctggata ttaattgata ttcatcctcc tttatatatt tttttaatgc tgaagatatt
     3661 gcccctgtgt tattcagtgc attaaggcac tcacaacaaa tatagtaggt tgcagaagac
     3721 cccgttttct ttccgtagtc tgtttctaca tcaatcaggt gtgtaagagc acgctttaca
     3781 tttatgccat catttaacat tccagataaa tcttcatata aacttattct ggaatacctg
     3841 aatgtgtatt ttgccagcca gtgggatatt ccagagaaat caatttctat cgaattgagt
     3901 ttctcaaata tatcttttat cgccatgtcc cctccgggag cattgagtat ttatcttcgt
     3961 caatggggga tatgaagtgt gcggacagcg gatctaccag accatgatta atcaggtgta
     4021 ataagtgctg attacgggta attcctccaa gttcatagtg ccagtatgat tttgcttcca
     4081 gacgcccgga ctgacgatat aactgaaaaa aacgtgcgtc gggtgagacg acttctgcta
     4141 taaccgtcct tcccgttacg cctttataac agtgctcgca accgtcaaga ttgcgcaacc
     4201 ggacctgttc cggcaggcaa ttttcctcaa tgagtttccg ttcttctgta ctcagtttgt
     4261 ctgccatttc agcataagtg cgcttacagt aaggacagag tttttgtacc agccgttgac
     4321 tcagcagacc gataaacaga agaggatctg caatcagcct ggcctgaata ttctccattt
     4381 caaggcgctc aataatattc agggcatcgt tggcgtgtaa ggtcgtcaac agaaggtggc
     4441 ctgtcatcga ggctttgatt gctgctaatg ccgatgcgtg atcacgaatt tcgccgttca
     4501 gtatcgcgtc tggatcaagt cgcatagcgg atttaatcgc gtcaacccag ccatcgacgg
     4561 aatccctgac tgccgtctgt attgcgccgg gaatgcggcc ctccggcggt gattcaatgg
     4621 tgaaaaggcg ttttcggggg agacgcatat tatcgttatg gttgaaacca aacgtgctca
     4681 gataggcttc tgaagccgtt ctcagagtcg ttgacttacc ggaccctgta gggccggata
     4741 aaacgataat tccttccggg cgttgtaaca ttcgcctgat tgtctggatt tgttgcggca
     4801 tgtagccaag ttcttcgaaa gtgggaatat cctcgctgtc atctttaatc aggcggaaga
     4861 ccgcgtacac accacccacg cacggataat gcgaatagcg cgccccaaaa agatttacag
     4921 gcttcagaaa attctctgcg atacgtgcat cctgtggtat tgcagggtca aactgcgtac
     4981 ctttgataac atcactcatt ccagaatagg cagcacccag caattccagc ccttctttac
     5041 gcgggatgac atccagaatt tccagttccc catgcacgcg tgcttcgaca taagtgaact
     5101 cggagccgtc ccgaccaggt gtgatgtgca aatcggaact tcccagtttg tttgctttcg
     5161 ccaggtagct aatgaccttt tcctggttgc tgtttaagtg ttgtgatgag tttgaatcag
     5221 accttttttc gctgtcctgg tagcgggatg cgataacatc aagagttgtc gaggttatat
     5281 ttacctgtcc gggatattta acattcagag tgccaatata agcctgaaca gcacgctgtc
     5341 ctttactgtt atcagcgata taaacctgaa caggtttatc gtctttttca gatatactga
     5401 cgtatacaac atcagaaata tcttttccgg gatagtctgc gtcagaaaaa aacattatgt
     5461 gtcgtctctt tcaatagcat taaagaataa gttggcgttt ctctgtacca ttctgaatcc
     5521 agacgccatt accagtgatt gatgccacct tataagaagt accaggaata ctttgcccga
     5581 ccttgacatc agttactgac ccgtccagga gagaaattcg tgctgacatt tgttgtggtg
     5641 taccaaatat ttcaataaca cgaagtgatt tagcggctac acgttgcgct ggtatatcgg
     5701 tatctgcaag agaagaatac ccggaagagg gcgttattgc tggcgttgcg gacgctacac
     5761 cattcaaatc aagattacgc aattcactga cagccttctg acgcgccagc tttgcttcgt
     5821 aaattaccgt ttcagcctga atctgctcta cctgtaaaat ggtcggagat gaatatttta
     5881 tttcgtcttt tggtgtgctt tcagattctg ttgcgtaagc aagtgaactg aaaaaacaga
     5941 cagaaataat cgttggttta attattttta acatagaatg cacctttaat ctcataattc
     6001 agtcttcctt cttccagttt tattcctgca tactggaagc gaacggcagg ttcatgaaag
     6061 ttagcgaaaa gtattgaagg cggggtgctg gtctgaatca ttaattcata ctcgttccac
     6121 gggagaatta ttggacggcc ttcttcatct gtttttgtgt tttcaatttc ctgccaggtg
     6181 agttttaacc tcattttttg tgcaaatgtt gtcaggcgtt cctgaatatc tgttgcctgt
     6241 ggcaatgtgt ccggggtaat tggatcaggc gaaacgtcaa aactgacagg aagagaaaat
     6301 cccccggcgc tgccttctgg caatacaaaa gttgctgtgg ttgtcccctg gaatatttct
     6361 cgtatccgtg tgctgaagtc ctcgaccgta acgccgctca gttccttata tgatgtgcgt
     6421 aacagcccgt cattaccgga tgtgctgcat tcagcaaggt caaaacgcca tccggcgatc
     6481 gataagggca gtgcatccca tttatcaatg catttatcaa taaatggttt tatttcaggt
     6541 aaatgttgcc acggtggggc aatctgtatt gcctgtttgt ctgccatttc ttttgccaga
     6601 cgaatacgtg cagcttctgc cgcagcttct ttttcccggt attcctggta catggtgatc
     6661 ccgttccaga gcagtatggc cagaatggca cttccgccat aaatcatgaa ctgacgtttt
     6721 cgggatacgc gagtaaacgc cgcctctttc ggcggttttt ttacgtcaat caacgcagac
     6781 agagtgagtg aggggagtgc ctggctgata tcccatgatt caggctggta tagtttccag
     6841 ccggggtctg gtgtttcatt aaactcaaga aatgttgtca gagcactcat gatctgactt
     6901 ttgttaccta caacatcgtt gacgagaacg ttattgacgc aggaaacaaa gccataaaga
     6961 tcaccgagtt cgcagatagc gtatccgtca ggttcgatga ggggttttat catcagtgcc
     7021 agtgaccaga atgaggatgc ccctgtggga agatcctctt ttctgataaa tgccaccatc
     7081 gttttggttt cattttctga atcctgatac tgacaggaga gaaaatattc agcgtcgttt
     7141 tttcttgcga atgtacgcgc attcttttct tctgaaggaa gcgttttcca gtccagaccg
     7201 gagaaaaaag cacgcttgtt tttaataacc gaagtgaaat tccggttatt ctgattattc
     7261 tcagacaaag tgacctccaa aaaaactccc tgtgtttttt cagggagttt tcagttaatt
     7321 agttcacatt ttttattact gcactggagc cggaattaag cacatttggt gtaatcaaaa
     7381 cgaccagaat cgtgtcgccg tcttcgctgt taacgccacc cccaagtcca aagaaggagg
     7441 gggttattac accttgctta ctggttttcc ggtttgattg ctggaatcca ctcagaatga
     7501 ctgtctggcc tgatttcagg tcaatagtct gattaattgt tttcaaatcc acattcggtg
     7561 tttgtgcttt actgccgcca ctttcgaaca cctcaaaagt aggtttgtca gacagactca
     7621 ttgagaacag caattgcagg ttttgtgaat ccggctgaat gtatggaagc agagtcatat
     7681 tgaatccggt cgttattgtt gccgcattca gtgacgtgga tgatcccaca ttggcggtag
     7741 aatcggttgt gacactttca atgaagccct gctgattggc aatttgcata gggacaggcg
     7801 tcaggttttt agtcacagcg gagttttgcg ttacgacact gacgctgcct tgtgacgaca
     7861 gggctttcag aaatgctttt gagccagcca gcttaccgtc cacgatggat acaccactgg
     7921 taataacatt ctcggcggca tcaccaaatg agccaccaag actcagacca agatgaccat
     7981 cattaaatac tgcgttccag tcgattcccg cctgttcctg gcttgatttc ttgatactca
     8041 ggatttcaac gttcagcaca acctgacgac tcatttcatt attgcgcttg ttcacaatct
     8101 cctgaacgct gtccagtaca tccggggtat ctgttactgt cagtgaacct gtggacagat
     8161 acatgcgccc tgtacctggt gtcagcattg aggaaacttc gcttttcagg tcgttgtaca
     8221 gcgatgattt catttctacg gttgtagttt gcgtatttga tgcgtcgcca gtcataccgc
     8281 cagaacttgt agagccggta ctgccactgg aagacattgt gccgctcaca acttttgagt
     8341 tgtatgccac attgctgtcc atatatgtaa tcgggaatga gcgcgtttcc agatagaaga
     8401 atgtgatacg acctttatcg tatttccagg atattcccag ccgtgaagat acggtttgca
     8461 gtagggcagg aagcccggag atattcagtt cagggagtga tgtaccgcca gaagagactt
     8521 ttactggctg actgcctact gacgccagag gaaccattcc atttgcatca ggtgctggaa
     8581 ttgagcctgt catttgttgt gttgagccag tgcctgtacc gttcagatag gtccagacat
     8641 cggcgctaat tcgtaccggg atatgacagg tttgtgtgat gcgttgtgcg atctggcgta
     8701 gctcaatatc tgaacgagta ttaatcacaa tcgggcaggg cggcgctgat tgctttacct
     8761 gcttttcaat aattggcgtt gtattaatcc attgttttgt tgaatcctgg ataaccgggt
     8821 tattacgaat ttttttcgcg tattcagtcg ctgtttgtgc gtctctttcg gcagcaagtt
     8881 cattagcatt gatttttttg aagtttgcac agcctgataa taaaaaagag gatattaata
     8941 aacaaagcaa ttttttattc atgtttaatt accttatgga atatgcaaaa tactatttgc
     9001 tggttgcgag gattattcag atacgtctgt tttatcgtga ttatcaaggt ctggattagt
     9061 aatcacattg cctccggcaa ccatgacttt ttttcctcca gctttaggga gataaatttc
     9121 tgcaattttt gctatttcat ccatgacttc ttgtgtaaac aggtttggaa tgtcaacatg
     9181 attgttacgg gcgatatcca cgcggcgttt aaactctggt tcttcaaacc agattatttt
     9241 attggcaata aatggtcggc taaattcact ggttataatt tcacggattg caatatttct
     9301 ggctttattc ttcttatccc ggagagacac aatttcttca ggaagaagaa ccgggcgctc
     9361 tattacatca tcattccgtg tacgtgaact tcctccctta ccaccaccac tggatattga
     9421 gcgtttggat attttcatat cacgcacccc aatctcttct gaaatctttt tagccagagc
     9481 attttttgat ttcggcggat agtaaagcac aacagcactg ttttcagtga atgtggtcca
     9541 tccttcctga ccgtacatat cgcttttttg cccttgtcct tcattctgaa gaatgaacat
     9601 aaagcgaagg ttataacctg ctgtaaaacc aacagcgcgt tcaatgacct ccgatttacc
     9661 cattgatgta aattcatcca ggagaataag acactggtat ttcaggtccg ggttgtgttc
     9721 cggtagttca cgacagtttt cattaaccag taatgagaag aaaaggttta caattttctc
     9781 atgagtgatt aaagcatccg gtgtcagacc aagataaatt gacatgggct ttttacgaat
     9841 atcccggata tcaaaatcac tgaaattcgt cgcctcagca gtaatagggt tgctgaagat
     9901 gctcagcggt gatgaaaagt ttgtttctat acttcccctt gtgcgatctg gtgctgacat
     9961 gaacttaaag aagaatgatt ttgttttatc gctaatccag ctacggtttt caatttcctg
    10021 tcccatccat gcggcgagat ccttgccgcc atcgggaacc gacgttttca gtatggcgct
    10081 gatggagaca agaacgtccg gtacattatg acctttggct ttctgttcca ggtgaaaacg
    10141 ctccttgtcc agcaggtaaa gtcctaaccc gacaaaaagg tttcgtgcag aatcagacca
    10201 tataggatca tcacttgccg ggataaggat tgcggcaatt tttgccaggt ccgtttcacg
    10261 aagtaaatca ctgcgactga cgcaatccag aggattccag cggtgagagc gtatctgtcc
    10321 tttaattgcc tggtcgatag tttccgcgta tccggcgggc gcaaagagaa agcattcttg
    10381 ccctaattct ttttgcctga atccggctga taaaaaccag ttttcaagtt tgatatccag
    10441 tatgaccata cttcctggat agtttacgca gttggggata acaatcccca cccctttacc
    10501 agaacgtgtc ggggcataaa gaattaaaaa ttgttgtccg gcgaaataaa taaattgttt
    10561 tttgtatctc cccttgaaca ttttaccgat gagtattggt ggatgtttgt aaggtgattt
    10621 tttatccggg aaaaaacctg atttactcag gtccatatca gttgcgagcc gggcgtcacc
    10681 gtagatgact tttttaggca taatcccaat cacgacaatt aacatgaata tggcaaagaa
    10741 tattaatgga acgctgaatc caaaccatgc cataaaaacg agttttttat attgtgggtt
    10801 gccagaaaat gttgagatta cagaatggaa tgtatcccac ttgagcaaag acatatcaac
    10861 ggatgaatat cttaatgctg tgtatccacc gagatagttt gaggctataa gcacacccac
    10921 aagaagcagt aacaggaaga gaattaaccc ccccgtcttt tttgcatcca tagttatctc
    10981 cagaatttac attgtgctca aaaagcgatt tttttcttcg ggttcgtaat acagttcagt
    11041 cattctggta tggttgaaaa aaagaatgac atcaattgat gtttttatcg tgcgcatgat
    11101 gaggtccata tcaacagtca taccaacatc agattgtttt acaatacttg ccagtcgtga
    11161 aaatgaggca taagtattat tagcatgaat ggtactgatt gagccttcat gccctgtatt
    11221 aagcgcctcc agataactcc atgcttcgtt acccctaagt tcagcaagaa agatgtgatc
    11281 tggctttaat cgcatacacg cttcaatgat ttccttcgct tcaacggtat tttttttgta
    11341 gaaaagccgg atgtggtttg gatgcagcgg cagagacatt tccggtacat cttcaacggt
    11401 tatataacgc cgttcaggtg gaaagatatc tgcaattgcc tttgctatag ttgtcttacc
    11461 ggagccagtc ccaccgacga ttaaaaaatt aagacggtct ttaactgcct ctcttaaaaa
    11521 ttgagcttta gattgaccat ctggacgccg ggataattca tacaggtagc gttgccgttc
    11581 agtcaggatg gcttcgtgtt tcgtcgcaat cctgacatta tcaaaacgcc ctgttcggac
    11641 ataatcatct atagtaaagc gcgttaatga tggcttacgg atagatatca caacgctgtt
    11701 attttctgtt gcaggtggaa tgatgatttg tccacgttcc ccaccgggaa gaactaccga
    11761 tgcaatggga ttatcatggc taagtggtat ttttatcttt gataagttcg tcagagtttt
    11821 ggcaagtgtc attaaattat caaaagtcgc gtctggcgca tctttagatt cccagccatt
    11881 tttcccttca aaccatattg ttcctggttt attcacagat atttctgtga catgttcaat
    11941 attaagaaca gactggatac ccgtcatatc cagatatcgt cttgcggttt tactgttatc
    12001 tcgtggttgt gatgtattac tggtatctct gatgttattc atatcaattg ctttactgaa
    12061 ttactgaatt actgaattac tgaattactg aattactgaa gttcatatac tgatgaaaag
    12121 tcgatgtttc gcggaacaat aacagacaat acagtcccct gattaatata tgccgtgggg
    12181 ggaatattta tcgtattgtc cagcgttgtt tttgccattt cttttgttgc gtcagatgtg
    12241 ttttcgtagg tgatattgct gtttgagcct gtttgttgcg tgctgttttt cagaatatct
    12301 aaaccatcac caagtaatga cagcagcaat gcaccaccaa aacgttgacc aaaatgatta
    12361 tcgacccatg caggaaggcc ggaagcgccc agactgtcag tgccaagtgc gccaatgcgc
    12421 acattgacat tttcatcttt gagcgttgtc cagttaacga acacacgggc tactccctgt
    12481 gtcatgactt tattctgctc cccgatcagt aatgctcctt tacgggcaag aagcacttcg
    12541 ccgttatccg accagacgtc tcgtgttaac tgacacattg tgataccggg ataagaggtt
    12601 acgattttcg ttttgagcac acaggataat gctgttcccg cagataacag atatcggcgg
    12661 ttcagcacag gggatacctt tccgtctgcg tactgactgc cctgtaaatc atctctttcc
    12721 tggttttttt cgcctggttc ctgattgtcg atcttcacca ttgtgtcgcc catcagttgt
    12781 cggacggatt ttggcaatgg ttgtggttgg tttggatctc cactggcggt ttggggagtg
    12841 ccgttggcta tcacattgac ggctgtttga tcggcctttt gtgatgctgg cgctgacggg
    12901 gcattgtctt cctgtttctg ttcttgctcc tcctgtgctt tcctgcggtt agcggcatcg
    12961 agtttttcct tttcttcgat gtttttcatc attgttgtca gtgaaacgcc attgttggta
    13021 tttgtgacca gtgtctcgtc agacttctca agaggtgttt gctgaacgac ctgttctcgt
    13081 gaaagaactt taaacaagat aatgatcaca aataacgcgg caatcagtaa tatgatgagg
    13141 attaatattt tttttggtgc tgctttacct tttacttcga taattccgcg atttcccgcg
    13201 tcattatttt tttcatcatc tttatttttc atagctgaac ttctctactc gtgtttttcc
    13261 gtttgacgta ccttttttat tccacggacg attaacgatt gatgatgttc tgatatctgc
    13321 aacctgatca cccaggcgca aacggagatt ttcagccacg tcatagtaaa ccattgtgtt
    13381 tttatttctg tctccgttaa ccagatgttc atttccatcg gcatcaacac gataaaggac
    13441 cggaatttcc tgtttattag tccatctcat gcatgtaaat tcgccattat cccatacctg
    13501 ataagggaaa attgatttgt cccccttgag ttgataatga ccattaatta ttccaccatc
    13561 agtgcatgga aatggctttt cagtcattac ggtagctgac ggtggtttcg gataattaaa
    13621 acgcagaata tacgtgggtt gtttcgtggt tgatatcaga taaagaggat atatatgctt
    13681 attggtctga acggtaacgt ttgtatccgg ttcttctgct tttggacgca gaaacagagt
    13741 gttgtccctg accgatactg accaggcttc tggatcacca attccgacat cttttattgt
    13801 ttcgtcttgt ccgaacttaa ttgttgtttg tgcgcctgct tttacgcgca cattaaatac
    13861 atcctgctcg ttataatcta cggtctgaat acgcccgtct cgttccgatg gtgtgccgta
    13921 catagctgct aaagcaccag gtacttgtag catcgacaaa agaactatcg cagtagtgcg
    13981 tttaagcatc attttataac ctctggatca ggttggtagc tcagaacttg cagtcccagc
    14041 ggattcacaa ggcgctcttt ttctgttttg atatctttat cccagtcaaa agaaatcgtc
    14101 gaaatccact ctgttgctct gtatccgggg gcgggtttac cagataaatc cagtaccatt
    14161 tttttgaaac gcacctgcgc catatccttc cttagaagga ttactgaatt aatctggacc
    14221 ttaattttgt agttgttttt cagaatgttc agtggtgagg aatcagcacg aatcatggtg
    14281 tcataagctg aaaacacctt ttgggaagac attgttttta ctgtatcggc ctgttcctgg
    14341 atagtctgcc agtcgtatgc ttcatagtta atgagatatt ttgacaggaa atatttagtc
    14401 tcaacatcct gataactttc cttagcatca gataattgag gggcaatatc agtatatcca
    14461 gtgctgttat ccactcgcac aaggaacggt acagcggttt ttaatggagc aagggaggct
    14521 attgcgaaac caagaatgca aattacaaca aattcaccaa tggcgagaat ggtaaatgtt
    14581 tttgcctttt tctttatgat ttcaatttca ctgcgctcaa agtcacgtat ggccttgaaa
    14641 taatcctttt tttcgttctt ttcttcaaat ttctttttgg aggcttcttt ttttgaatgt
    14701 tcataatagt ttgtgttttc cattaataat cacctgcttt gatctgttca attgctgtgt
    14761 ttaactcaaa ccatccccct tttttctgca caggattggg ggtgtgagaa catgcgctaa
    14821 ttaatagcgc ggaggcaatt atgaggggga ttttcatatt gttttccgtt ctgtataaaa
    14881 cagagaaatt aaccgttcag gcgtttcagg agttctggta tccagatatc aggagctaca
    14941 tctccttttt cagcaataat ctcatcaaaa atagacaggc cagtttcaga gccagaaagc
    15001 acggggataa actcatcgaa tccaaacaaa tccattttgg cgaaaacaga agagccagat
    15061 tgcttgatga gcatggtcct tgactccttc gtcagctctt tgagtttttc aaactctttt
    15121 tcagtaagcc caatttcttt atatccttcc cattttgcat cgggattggg caggagaatt
    15181 ttcgttgcgg tttgttgtac cagcgccgca aaaattgcgc agtttatggc gtcttccggg
    15241 gactgtgatg ttaaccacat catttcacct ttcatacgcc ccgctttcag tgcgctttta
    15301 atcatgcttt gtgtcatggg aaagtttgct ggcatccaga actcttcaat aatggagagc
    15361 atcagattgc cgccgcgttg catgatttct ttgtagaaaa acaggaccgc gagtaaaggt
    15421 tcacaggcag gatgaatacc gttttttgtg tccaggacaa cggttgtatc aaatcccact
    15481 tttttatgat gcagtggatt gaaggtattc cggggggaat ccaccgccca ggcatattcg
    15541 ccgttgtcgc accatttggc gagacgcgtc tgaagttcag gactgactat atcgagaaga
    15601 agggctgtcc ggcgttcttc gacaggcatt cgcataatgc tttccacggc agcgtttaat
    15661 tcaatgccat gttcatcaga acaggggttg ccctggttgt ctcgtgccag aacctgtgtc
    15721 cagcgtttca gaaatgcgag aagtctgtgc cagacgggag attccgggcc tgctgccagt
    15781 tgccacggat tgcagcctgt ataaatgcct tcctgcaacg tgaaatagct tcctccgtat
    15841 gcacggacaa aaagctcagt agaacggttg aaatccacaa caaacattaa agggtcaaag
    15901 cgttgaagaa aaccgctggc tgctgcttca aatgttgttt tccctgtgcc tgttgcgcca
    15961 agaatcattc catgcccggc aattttttgt ccggtaacat ttttttccgg tgggctgtaa
    16021 tgcgtattga accagtaaat gctgtcagac accgttttca acggcattat ggcggaccca
    16081 tcaccaatag gattgcctga ttttttcccg gaggaataat tgtgaaaaga catcagacag
    16141 gccaggttgg ttattgttct tcgggtatcc agtggacgcc tcttattcag cggcatatga
    16201 ctgaaaaaaa caaaaggtga tgcgagtgag gcgcgactga agcggaatcc cttacccgaa
    16261 gtaataaact cagcagagag ctttatacca ttgcttctgg cctgatctgg tgtatcaccg
    16321 aataccgtta atgcgcagtg tagtgaacca aacaacgtaa ttccggctgc gaccgcttct
    16381 ttaccagctt cgagttcttc ctgttgtgtt ttggctgcat cattagctga ttgcagtttg
    16441 tttaattgcg aatcaatgtt cttcagggtt tttgtgggtg attcaaaaat aaacgattga
    16501 gtaagaataa attcataggg ttgcttgaga agaaaatccc attgtccggg ggtcgtttct
    16561 atagggaaat ccttcaaaat ataattcgtt gcaaactttt tactatccga ttcgttgtta
    16621 cgcagttcaa gaacatcagc accaaaatac cattcagaat cacaaatact ggatgataag
    16681 ggagtggatg ataaaggaat catgttatgt tctgtattta gcaatagtga taaatagtct
    16741 gccacctcac taatataagt attgtgaact gataaaacag aagcgttaaa tggctgaagt
    16801 gctttttgcg cctgttgtgt catttcattc atgcgctcga ttccggtatc aacatcatcg
    16861 tagggaatcc caaatgttaa ataccatgtt gatttgtaga atgcgctgga tgtgaacaga
    16921 gcaaggtatt tttttgaaaa cctgcttaaa aaagcattgt caaaattcca ttctccatca
    16981 atggttattc tttttttgat aagatgagtc cataaataca ggtcgccttc ctttcctaac
    17041 ccaatcagga agtttttaac gctgttaaat aaatttgtaa gaacattatc gttctctgtc
    17101 tcaaatggaa tgcctgctac aacaagtgaa gccagcattt tcctgtcaga agtaaagata
    17161 atactgtctg ttatatggaa gcgataatcg ggatattcac gagcaacatc catattgtca
    17221 ctgtttttta atttgatttt gtgcatatat gttacctgac agatgaataa ccaataatta
    17281 aatctctgtg gcggattttt aacaaaagcc ccataaggtt taaacggatg actcttagtg
    17341 cgtttgagtc attttcacag gccagtttta ctataaaaag aaacagcgca catatgacag
    17401 gaataattat cccttttata ccccaaagga aaatagcggg gaagcctgtc agaacagcga
    17461 aaaaagccgt gaacaataac aacattaagg gtataccagc aattaacgca ggacggttaa
    17521 acccgttata agtcatgaat gactcactga catgttcatt tactttgtcc ataattcacc
    17581 atgaaaaaag ccgcaattca cacataaggt aaatatgcgg cacagttgta tatatggatt
    17641 acttatttga agagttccag cgcccagttc ccggcgtaaa gtgcaccgcc agcgagggaa
    17701 caatagacaa gcgccattcc tacatcagac cagcttttct tctccatgaa tgccatgatg
    17761 acgttataga tcaggtaaca caaagcacaa acaccaccaa agctaaatgc ccatgtctta
    17821 agttctgtca tggtatttgt tgcggtgtcg agtccacctg cataggcaac ggaggcagtt
    17881 agtgacagga aaaacgccag aataaaatac tgcaaagttg atttgacttt cattaactaa
    17941 aatctccaaa aacatcccag tccggtgatg ttttctcttc atcattcggg gggtttattt
    18001 ctgattcatc agttttttca cggattgatg gcacgatata gttattcgcg ttacgaataa
    18061 cccgttccag ataagaagtg ttgttaaatt tcttttcttt aataaatcct gttgattcgt
    18121 tacccgaata atacaaagat aaagcgcgga gaattttttg attactacta ccctcctttt
    18181 gtctcagata attgtccgtc aggactgccg ctcctgcttt tatatttgta cacgggtcaa
    18241 aaactgtttt atttgtcaga ttgagtgatg ggaaattttt gctgtatatc tgtgtcagac
    18301 ctgcactaaa ccttttattt tctgctgtta atttttctgc atattcaata gctccttttt
    18361 catctttaaa atacttactt gttccatcag aaacattcgc tattacatat ggattagccc
    18421 ctgattcagt cataatcagg gcattcatcg tatcgggtga tacatcaggg gcacactgac
    18481 ttatcagtgc tgccagaaca gccgctgata actgcataag ggatttatct cctgatatta
    18541 aattcgtgaa gaacgttttt tcctgcgggg gcgacgaacg atactgtcca gctcattagc
    18601 gccggaaaga agttctttca gcgagacaat tttttccctg acaggtataa gggattttgc
    18661 ccccggacat tgttcaaagt ctgatttgta caggtcgtat aaaacaagcg tatgttttac
    18721 atcctgtgac agataattat gtttattctg gatgttacct tgttcgatat ttttgataat
    18781 atctgctgaa cgatgaatta aatctgtgta gattcgggtg tttttaagag gatttatttt
    18841 tatatattca gaagcaagct gaataagatt gcatcgtaag ataaggactt cacgagtggc
    18901 aatatcaagc tcgctgacat catcattatc ctgcatgggt atctccggta aagattttct
    18961 ggactgaagt ccgcgccgta gcgatatatc cggcccacat aatgatgtgt cggatatgca
    19021 gactccagtt cagaatcccc ttgtcagggg atatttagtt aatgagtaca atagcctctt
    19081 ctggaatgga ggaaggaacg ggaacgccca tatcattccc ctgattatcg acaatcttct
    19141 tattttttac tcttcctaac attgctgaat gttttgtttg ctgctgcaaa gcagacatca
    19201 gaccttctgt atcggcagac cagatataaa ctcgcttacc gtaaataata tgatgaatat
    19261 cataaacggg tgttataccg atttgctcag atgttaacgt tcctcctgaa ttacgtttgt
    19321 ctggatattt ataaagatag tcattgataa catttgcgaa atggacaaat tcaattgctt
    19381 cctgttttgc aggaagcaac ttcattcgct gtatggtttg gttattgtta tctgtcagat
    19441 agtaactgcc tattgacagg aatatcagga gcaagctgaa gaaccaaccc atagcgatta
    19501 ctcctgttgt gtgtctttaa ttgtgattac gcattgtgca atattcctga atgcgtataa
    19561 cggtttatct gctgaaagat aaagcatcag cgtatctttt aatgcagatt caaagtttcc
    19621 ccggaaaata agaggagcat ctattctgta gttcacagga gtatcccata caagattcca
    19681 ggttgaatta ccacacggcg aacttgacgc ccattctgtg aggccatccc gcagcgtcga
    19741 acctttttta agattccagg tgagtttttc tgtctgtttt tgcggaacgg tgacaggcgt
    19801 tagcgatttt tgaggtaact tgtcagagga aacgggtgtc ctgacaggag atgcaggggt
    19861 tttgtcagga gatacaggtg ctttttgtga tgtttttcct ttctgttgtt gaaaatcaat
    19921 cagaagatgt cttttttcgg tgttcttaat taccgtcaga ccgtagttgc gaagcatttt
    19981 atccaggaca taaggccatt gatcgttgcc ctgccaggat atcgtgcgac ggaaagtttt
    20041 cgttacctcc ggggatattt cagccgtcca gtccgatggg acaatttctt ttaatgcttt
    20101 ttgcagcgag cctttaccat catggcgaac aaagtaaatt tgcttttcag gtgttgatgg
    20161 gcctgtaagc gtggtggccg gaacagcgtt gttagcgttt tttttcagaa aaccgctttt
    20221 tgacattagc ggtggttgtt caggacgcgg ttttgtctgc tgatcagtct tttgtgccgt
    20281 ttgtgtctga tggttgttgc tactgattgg ctttgcggga gagagtgctg gttgttcaga
    20341 atgctgttta gttgctcctt tggctgctgt ttttatctca ccagatgaag ttatgctgtt
    20401 tgttgtcggc ttcagaaaac tattattgct gccctggttt tttggcgctg tcgctgattg
    20461 ctcagaacgt ggttttacgg tttctgcaat acgaggcgcg ggagttacgt ttatggcttc
    20521 ctttttgaga taattatcat tgctgtattg aacagtgctg tttggctgtg acacgggctg
    20581 ctgaaggatc accccgttat agtatgtgtc tgctcttacc tgctcaattg tggtattcag
    20641 ttcataccaa cctccttttg cctgtgcggg ttcaggggca tgggaacacg cattcagcaa
    20701 taatgtggtg ggaataataa tgagcttttt catggttaac cccaggaacg tccgaactta
    20761 ctcatgattt ttgagatcgc cgcagggtct gcggtcgtgc attgattaag aaacgctttt
    20821 cgggcatcaa aggttttgct ccagcgaata gatccctttt tcttttttat gatattaaag
    20881 aagtcctttt cggcgctttt gcattcgctc ccgccgccgc taccgatagc tttaccgtac
    20941 agacaaagaa cggaagcaca cggatcagac gcatatacgg gggcagagag tgcgatagca
    21001 cttgcggcga ccatcgataa aatgatggat ttcatggtgt tcctccatat ttcagacaaa
    21061 agaaaacccg cacgaggcgg gtaatttaaa tatcagggga atcaacgcac gtgtggcgta
    21121 atgatgacaa tccggttctc actttggcta tcagcattaa ctccagtaaa accactcacg
    21181 ataaggggct ggtccggttt gagtaaaata ttctgctcga cagtctgttt ccgtatcggg
    21241 tcagtggttg tgagtagcag atggaaattt ccacgctcaa tctccggtgc tatccgcatt
    21301 gtgaagccag cagtccgatc tttttcagcc agtgtagtgt caccggatat tgcctgacgg
    21361 gggatcagtt ccgtggcagg ttcccagctt ccggcagatg ttgtttgtga ttcaacgcac
    21421 ggggtactct gcattatttt gcggattgtt tcggaggata tcgcctgacc gtcatcaaag
    21481 aatgcactac ggaaaatcac gttctgattt ttggcaaaca agcctggatg ccgctcgctc
    21541 agactttcgt atatattcat accattcaaa caaacaggag aactgtccgg caggtctgtt
    21601 cgcacggtat caattttcaa cgttatctgg tggtcatttg aggttaacgg tgactcaggt
    21661 actggttgtg ttttgctatc cgcgcagcct gacagtaatg cggtggcggc aaccatcgat
    21721 aaaatgatgg atttcatgga gttcctccat atttcaggca aaagaaaacc cgcacgaggc
    21781 gggtttttta tcatggcata agtttattta ttggagcgtc caagcattta actcatgatt
    21841 tcttttcgtt ttagatcata gagtgcttcg ttagcatttt atgtattcct taatcaaggc
    21901 gtcttctcat ttcactaact ttctttttat attcactatc ttgtttttgc tgaagtgcag
    21961 cacgagcatt atcgcagttt tgatcggtta ttgtaccttt cttgcaatct tctaaagttt
    22021 tagtggcttc actgatgttg tttgagtaat attctacaga atatattttt tctttacatc
    22081 ccactaacat taaactggcg aagataattg atgaggcgat aattgcattt cgtgacttat
    22141 tcatatcagc ttaaatctct tctcttttgg cgaacatatg tttcatattg agctttccgc
    22201 tcattctcta gttgccgttc agcaatatca tattgcttca actgtagctc cattgtcaat
    22261 ttttctgtat tcaattggtt agataagtca gctttttttt gcggagtatc tgctgacgcg
    22321 aactccttct gtaacgtggt taatctctta gccctttcct gcatttcatt gttgaaattt
    22381 tcatagaatt tgatcttagc caggatcgca tcatattttt gttgcgtctc tgcaacgttg
    22441 cttgataagt catattgcga tcgcaaagag ctaagatctg attttgcact atttgttatt
    22501 gttgatagag aatccttcat atagctggat gcgctatcaa agccattgct gaaatcagtg
    22561 aagccttcga gtcttttctt ctgttcttca gcgtatctta tggtctgttc atattgttgc
    22621 ttgagttgct caagctgtct tgccgcttca gcagcacgat ttagcccttc ttcaactgtt
    22681 ttagctattg ctgcaacgtc tactactgga ataccagatg caaagattga tggtgatgtt
    22741 atggtaatta ttccagcaaa gagaaatgat aatactttat ctttcatgtt aatcaccccg
    22801 ctttaagttt acttccacgg tttaatccct gaactaatgc tgatgcagaa cgacttcctg
    22861 cattaaagaa tggatttggc attttgctgg caaaaccatt agtgaaagat cggagtcctg
    22921 atgctcgcat aaaaccggat gcaaccttac ctccaaagcc attagcggct gatgtaaggc
    22981 catttatacc taccccgcca gtaagagttg agcagagagt acctacttgc tcaacaagga
    23041 atatagatat aacagtcacc ataaagaaat aaactactgt tgtgagagtt atatttccgc
    23101 ttgatgggac tgtttgctct acaaagctga tgacgaagct gaacgatata ctataaaaaa
    23161 gatttagcaa tatataattt aaacagctac ctacccatgc gctaaacata tttcttgtcg
    23221 aagcaaatag agaaaaacag ataaataata ttccggcgga taagagaatg ccaaccatga
    23281 atgttgatag agttagaaat acagtggtgt aataaataag cagcagcccg ccaacatatc
    23341 caacccccca aattagatat atgtatagtt gatctgtgaa gctataccac tccagagtat
    23401 ctaactcatt attcttaaaa tcatttaacg ttactgaaag catattccac aaattatcaa
    23461 cactggttgc agttcctgaa cctccagtaa cagcagcaga aaggtcggag ccagcgtgca
    23521 ttacaaaagg aataacaaac tggctgtaat aaggcgctga ataagtgaat gcaccaacca
    23581 gggcgaatgc accgatattc ttagtcacct cgctcatcag gacgtcacgt tgggcatacg
    23641 tgatttcata tattagataa ataacgtata aggctatagc agcaaagaat gtaggagata
    23701 ttgcctgtgc gaccttagcg gcgttcgtcg ccgctgtgga ttcaacaatg gttgttactt
    23761 tctcaagaaa ttctgaaaca atgttcatag cttagtcctt ctggcagatg aagagttcac
    23821 cagaggcact aatatttggc ttcagcttaa ttaccaggtc tgtgccgaaa aaaattgctt
    23881 ctccaatttt ttggaaattt aatgtggatt cttctttatt gtaactataa acgttgtggg
    23941 tcgctgtcga tgttttggtt ttgcgtggtg taaacgcttt tttaatgatg taatcgagag
    24001 cgtggctatt atgagatggt aaagtgatta aatcgtcgtt ttttttcata atgtagttct
    24061 gatttttgag gttgaagata attgtcggtc aatgcttttg attttttttc gtatctcaat
    24121 tggattcgcc tcagataagt tagcaatgga taaagccagt tcgatttgta aaattttcct
    24181 tgtttcgatg agattgattt ttacttgtgg tatatcacgg aagacaccac agccggtaat
    24241 tgcttttgaa acgacacgtt ctgtattcgc tatgtttttc tttactgttc gaggggggac
    24301 ataacgtgca cggcgaagtt ttctcagatt agcttttgct tgagtcatat ttgtatgccc
    24361 tgaataaata aatgctttct tcaaagcctg aataagcata aaccaggatt atggtggcta
    24421 aaggcaaaga aaaggtgaaa tgaataacgg cattcagaac tgaaccggtt aatgtatata
    24481 agacaaacgg tgctgaaagt atgaaagtac ccagcaggaa agaaaaaaca ttgcacgcca
    24541 tttttaataa aaatattttt attaggtgat gtttttttct gtacatttca tcgtatcctt
    24601 cagggggctg tcaccctctc acgttgttgc tttcatcagc aagtgcctca ataagtttat
    24661 taatacacgc ccgttctgcc ggaacctccc ggcctgtaat cgggtcaaaa acgacatgat
    24721 cgctttcaac gtaacgtaaa cggtgataga catctatacg gagttttgtt ttagcagctt
    24781 cacgttcaac tcgtgttaac tcttcttttt ctcgtttgct gctgaaaaac agaaaccaga
    24841 tcattgctat gataattact aaaaaaggga caacggtgcc taaaatgctt accatttctg
    24901 ccatgccacc cagaatagat tgaatctttc ctgtggctgt ggtggttgtt actggttcag
    24961 caagatcaga accagtgtct gataaaaatg taaataaacg ggcagataaa ccaaatagca
    25021 cgccaaaaac gagaactttt attatcgaac cgtatacatt taatagactg tattcttcct
    25081 ctttaggcga ttcaaagttt tttgcccttt gtgtctgctc ttgtttagaa ccagaaacac
    25141 ctccgatgaa taagccaata ccaccagtac ctaaaccaat acccccacca gtaccattcc
    25201 atttacctgt tgttgtttct ttagctacaa tagctgcaag agaaatccct tcatttgcgt
    25261 ttcgcataga attgtcctca tactattcct gtgacactta acaccatcaa taaaatatgt
    25321 aatgcagtaa tatttattag cgtttttttg tagacttcaa taaaatcttt atccagaagt
    25381 ctgataataa aaatacatag tgcattcatc gctgttcccg taagcggaat taacaacgaa
    25441 agaggcatgt tttattcctt gctttcactc ttgtcttcga tggtcattac tttaagagtt
    25501 attccttgca tgtctgtaac aataactctt gttcccgcag ggtagttttc atcgtgtaac
    25561 gtctcaacag gccagaatgt atcaccaatg agtaattgcc ccttgccgtt aacaatatcg
    25621 tcactgagcg taaattcttt tcctttaaaa cgtgaatgcc ctgtgttcac caggagagta
    25681 tctaatttct tatgtttttt gtcatagaag aacttaatta tcgacgccag tatgctgatt
    25741 gacgcaaaga agcatatatt ggcttcttgt gatatcggaa gaaaaaagcc cacaatggcg
    25801 cttgataatg caccaagact gattaatagt aaccatcctg ttccggttat aatttcaagt
    25861 gcaatacaca gaagaaacag tgtaaaccat aacatggcta atctccatga gataaggacg
    25921 gtgattaaaa caatattgtc accgtccgct atctttttta agacaaatat tgttgagcct
    25981 gtttcatatc aactttggga gtatcctgtg atacgttttt tagcagttca ctgatacctg
    26041 caaccgaact caccaggcta cttgaatcaa gcggcatcat aaccagttta ctgtttgatg
    26101 ctgtgccaat tgcctggagt gcttcagtgt atttctgcgc aataaagtaa ttaacagact
    26161 gaacatcacc ttcagcgatt gcatccgaca ccagttttgt tgcgcgagct tccgcttctg
    26221 cctgacgttc gcgtgcttca gactgaagaa atgctgattg ccgttcccct tccgctttca
    26281 gtatttgaga ctgtttttcc ccttctgcct tcagaatttc tgactggcgt ataccttccg
    26341 cttcaagaat ccgggcacgc ttagttcgtt ccgctttcat ttgcgcgttc atcgcttcag
    26401 tcagttcttt tggtggttta acatcccgaa tttcaatacg tgtgacctta attccccacg
    26461 ggtcagtcgc ataatcgaca accgttaaca gtttagaatt gatgctgtca cgttgagaaa
    26521 gcatatcgtc gagattcatt ccaccaacga cagttcggat attggtcata acaagatttg
    26581 aaatagctga tgcaagatta tcgacttcat aagctgcctt cgctgcatca atgacctgga
    26641 caaaacaaac agcatcgata gttacgtttg cattgtcttt cgaaataact tcctgtttag
    26701 gaatatccag gacggtttcc atcatgttga tccgttgacc tatgcggtcc ataaacggga
    26761 tcaggaagtg aagaccgggg gaaagcgtat gggtatattt accaaagcgt tccactgtcc
    26821 aggcattacc ttgtggcacg attttgacgg cagatttaac gaatatcagc gcaactaaaa
    26881 ttaataagag taacggaata gatgtaacta ttggcgtaat tatagcgtca atcatgatta
    26941 atcctctccc aattcatcac tgtggatgta tttctttgca aatgaaatct gaaatatgaa
    27001 catcaacaga aaaacacttt cctgcttctt tttcttttga gaagtttttt ctgcattctt
    27061 cagcggtcat agggaagtcg ctgtaatgag tccatttcac ggggccacca cctggttttt
    27121 tgatggttgc gtttatttgg tacttaggca ttcaaaaata cctccactcg atcacctgtc
    27181 tttcttgaac acatccagcg gcgcagttct gtaaaactat cctggatctc acaacgggta
    27241 cttttcattg tgacagttgg tatattgtcg tagctggcat atgcaatctc tttttttggg
    27301 gtgcgcaata tgcctttgtc aatatcaaga atcctgaacg cgctaattct tcttttgtgc
    27361 tcgttaaata gtgttcgttt agttgctgtt atctgcttct gtgcttcctg gtaaatgtag
    27421 gtaagcacat cataagattg ttgctgtgta ataaggaaat tacggctaac atccgttgtt
    27481 gtgagcgttt tattatttct taatccccaa taagccacga tgatatagag tggatatcct
    27541 ttcaaatctg ccacacattc aggaatgaca ggattaattt ttaacttacg cgccatattt
    27601 tctcccggtt gtttgtctga aatagaaaca tccccggaat acagcgtcat aagctaatta
    27661 tgaaaagttg cagccactca ctacaggctc tttatctgat tgttcatcat catgcatagc
    27721 taaaccccct tacctggagt ggagactggc aaatctggtg tacttctgcg ttgcagcctg
    27781 tacgacttgc tcagggagag agtgagtgag attaactcaa cgcgagttca ccaaataagg
    27841 gggtttaagt atgcagaatg atatttgcgg ataaagagtc gttacctgtg tagatggcgc
    27901 ggcacgtcac tctgttacgt agcccgttgc tgacagatgc cagcggtgct tgaaatgcac
    27961 gaataataac ctccttatgc cagggcttta ggcaatgacc gctgtccggg gttgttaaag
    28021 agcatgagat gacaagcgtt ctaacgggcg tcaaagagtg ctcattttcc aggcaatgag
    28081 cacaaaaaac gcgatgaacg agctttttac aatcatctgg atagtattgt gtgtgcgaca
    28141 tttaaaacct gtcaatgcca caagagcgat ctaatgatct ggataacacc taaaaaacag
    28201 aataatattc cgtttgttaa atgatgtacg atctaaaaat ctagataata ctgtttgtgt
    28261 ggtttattcg atcgtgtgag tgggtgtatg cagtcaaaaa aacttttttg aatatacaaa
    28321 attctgaatt tctgattttg taaggttttg tatggggaac gtcagtaaag aaggggcttc
    28381 agtagctgtt ctgaaaacga ttttgaagct gtcagagatg tgtgctgaat caaaaaggaa
    28441 acgagccagg cttgaacatc gttgatcgat tgcgcccatg ctgctaaatt tgcaggcaag
    28501 ttttccgcga tttgtaaatg cagaaaacga ggtgctgaaa ttttgttcaa gaacgtaaat
    28561 ttcagataaa gaaaaccccc gtaatctcgt gttttccagg cagcgaagat tagcgggggc
    28621 tgacatcaag gcagagcctt gagcttattt atttcaggta tgcttgttac ctgaagtgga
    28681 gtttaattcc atgagaagga aatatcaact cttacagtgc aagctaagtg cagcttgctg
    28741 tgattagcaa acgtcactgg tctgaactat caccggaaga gcaggtccgt ttctggcagg
    28801 actacgaagc gggaattgaa agctcattcc tggtccccca ggaaaacaaa ggcggaacaa
    28861 caaaacgtcg ccggggtgaa cattccacta agccaaagtg tgagaatcca gcctggttcc
    28921 gtcccgacaa ctataaggcg ctgggcggac agttgggcca cgcttacaat cgtctcgtta
    28981 aaaaagaccc ggtcactggt cagaacacac tcagaatgca catgtcgttg catccatttt
    29041 atgtactgaa acgtcagagt gtcggtcgca aatataaatt ccgaccggag aaacagcggc
    29101 tgcttgacgc aatatgggtt gtacttgtga gtttttgtga tcgtggattg cacactgtcg
    29161 gcatgtctgt ctcccgcctg gcagaagaaa taagcccaaa agacagtaag ggagacgtga
    29221 ttcctgaaac cgcagtcaca gtatcgcgcc tttcccgtct gctggcagag caggtttcct
    29281 ttggcacgtt gggaacgtca gaaaagacga tatgggatcg tgaatcccgc cagcgactgc
    29341 cgaaatacgt ctggatcacc gaaactggct ggaaaatgct gggagttgat ctggtgaaac
    29401 ttcaggagca gcaacgtaag cgccttgcag aaagtgaaat ccgtctgcaa ttgattaaag
    29461 aaggcgttat ccgcgagggc gaggaaatct ctgttcactc ggctcgtaaa cgctggtatg
    29521 cgcagcgttc tctcgatgcc atcaagtccc gacgtaagaa agcagcggag cgcaaacgtg
    29581 ctaatcgcct ggcaaaactg ccgtatgatg aacagcgaaa cgaaattgcg cggtttattc
    29641 tgaagcgtat gccgccggac gaagcgtact ggtgcactaa agaaagatta gagcaactgg
    29701 tagccagaga tctacgtcag cttgaactgg ctctgacagc ttcgccaccc cactagttta
    29761 gctgcctgat acaatcggca ctctcttcag ccccttcggg gcttttcgtg ttgcctgcat
    29821 ttcatgaaat tcctcatatt tgacgaccgt ttttcatttt gtcccgctct gacagttttt
    29881 tgatggccgc aatagggtat cggagtaaaa acgccttcgg ttatccacaa aaacccgcaa
    29941 aggaataact tcagaaagca gcaaacatag aacatcaaac ccgcaatata ttcttaaccc
    30001 ctgttcttta atcccctgcg ttgcttcgcc gcagggaaaa tatttatctt tgaaacaact
    30061 gtggataatt acaaaaatgc cttcgcttgc agcggctaac gccgcgccgc tcagaatatt
    30121 aaaagtacct cccaccgctt cgcggcgggc atatggactt ctgcctaatg aaaaacaaag
    30181 cccctccaac cctcctacaa aaaaacgctg caaatactca gccacagcgc ccaacccatc
    30241 actgaaaagc gccgccgccc ccgcccgaag ggcgggaaca acatcgcttt caataatgga
    30301 tgttgtaact aagaaatctc atcgctgtca gccttctggc tggaagtacc aaatacacgc
    30361 tcgtaagcgg ccctgacggt ccgctaacgc ggagatacgc cccgactgcg ggtaaaccct
    30421 tgtcgggacc actccgaccg cgcacaaaag ctatttcatg gctgaaagcg ggtatggctt
    30481 agcaggatgg ggatgggtaa ggtgaaacct atcaatcagt accggcttac gccgggcttc
    30541 ggcggtttag ctctgtgcca tataaaacaa cagagtgccg ccttacatgc cgctggcgcg
    30601 gcatcaaaaa gaaagccccc caataacggg gggaaacagc gttttgatca aaatgataat
    30661 gctagtgtgc ttcttgcgtg ccattccagg cacaaactaa atttaacgtt tcgttaacta
    30721 caaagaaatc cgatgagtta atcacttgcg aaataaaaat catatagtaa attactatat
    30781 gattttgcgt tgtttcggag aattaaaaaa tgcctttgat tgaatacatc aacaagttct
    30841 atcgggggaa tcaggcatcg ttcgccaggc tgaccggagt tcaacctgcg caggtaacac
    30901 agtggatcaa caaaggattc atcgtggtga atcacacact gtatagtcca cgccgaaagc
    30961 tgggaattta attcctggtc aatatataat gctgttggcg cttgaggctt tctgcctcat
    31021 ggcgaaaagg tggtttgtat cttgttgtgc ggcagaaaga agaaagcccc gtagttaatt
    31081 tttcattaac ccacgaggcg atcccaatgc ttcaacaaca tcaggatagc ctcttaccgc
    31141 gcgttacgca aggagaagaa ggccatgaaa cagccgaaaa acgccctgac ctggtgttta
    31201 ttaatcgtgt gctgcacatt gttaatattc acgtatctga cccggaatcg tttgtgcgag
    31261 gtccggctga aagatggaaa cagggaggtt acggcaagtc tggcttacga atccaacggt
    31321 aagtagcaac ccggaggcgg gcgaaagccc gcctttttgg ggatgatgtg gttctggcat
    31381 taagcgcctt tgcaaagggg gtattctgta cggatacccc ctttttccat gtattttcag
    31441 gatattaact gctgaaaatg gtataattag tcaatatgac agcaattatt gaggcgagta
    31501 tgcagccaaa aaaaacaccc gtgattgtgg tcaaaaaacg ccgcgttctg gtcatgccag
    31561 aaaatcctgt ggtgaatgaa aagccgcagg aagtacaaaa atcagcagtt aatgaaaaca
    31621 agaaagtaca gaagaaagat gctgtagcag agaaaacaca taaaaagcag cctcagccct
    31681 ggtatttgaa aaaacaaatc acttttcccc caaaatatcc gaaagaatat tttgaaaagt
    31741 gtttcaataa agttcgtgct gtttttcctg aactatggac agacgaaaaa aagaacttgc
    31801 ccctgaaaag cgggattctc caggacgttg agaaatacct ggcggataac ccggatgtgg
    31861 atctgacgat tgaagagtgg aattgtgcgg ttcaggtgat gacgttccgg tggcaatatc
    31921 ttcagaattg tactgtacca ggcgcaacac gttacgacct ttacgggaaa cctgctggta
    31981 cagtaaaaaa agcacacgca acttatgcgc aactggttct cgatgcccgt aagaaagcca
    32041 gcgagaagaa acaattaaaa cgtaagggat aaagccccga agggctttat catttcagcc
    32101 tggctgactg tagaatccag tgattttttc cgttttcgtc caggccgtgt tcgtaagctg
    32161 caagagccgc tttcattatt gctgtttttg tctggcctgt tcgcagtgct tcccgttcca
    32221 gaatatcttc gtaaacgtcc ccaagccgga cattggtcac tttagttgct cccgattttt
    32281 tgcgggtagc ttcattgatg aatcgggctg tgtctgcatt ggtttgttct gttttttgtc
    32341 ctggtattac cggagttttc aagtcaagag ccattatatc acctttcgtg tgttacatta
    32401 gctttactgt gtaaagttaa tgatgtaaag ctatcgtatt acatccatta gggattgatg
    32461 cctaactctt gcgccaaaag ctctatttgt gctttagctg tagaaaggct ggatgcgcga
    32521 cgtacatcat gaacgccagc gccttcatta caggccgctt caaaaatatc taaatctgaa
    32581 atacgtgtac gaagtggctg tatccacacc gggttctccc ggagtaactt gtcgagatca
    32641 atcgctgctt ttctgtggcg tggttttgtc gtgttaattc tcgtgaaaag tacccagggt
    32701 tgcaggtctg gattgatctg ttgagctgtg cgaacttttt ctgttacatg agtcagcgtt
    32761 tctgcttcga aatctgaaga cggcttaacg agagtaagca gaatgtcagc tacagttaag
    32821 gcactgcgaa attccttgct gtcatgccct ggacagtcaa tgatcagcac ttcacatatt
    32881 tttttcaggc gcttaatgac ttctgataca tcgccatact cttcataaac aggaaccggt
    32941 aataagccat tctgctttct tttttcgttc cagctaagaa tatcattgtt tttatcggtc
    33001 ttcagaatga tgaccgttcg tgaattattt accagataag aagccaggtt tgttgctgtg
    33061 gtgctttttc ctacaccgcc tttatctgat gcgacaagta atgctctccc catagaaacc
    33121 tcggatatac agtgtaaagg tttatttgtc tttacattgt attcattata gttacgttgt
    33181 aaagatgttt gtgtctttac aatgtgattg ttttttgtgc tttacattgt gtgtatgtta
    33241 cagggtaact tttcaggatg cgattttgcc tggtgtgttg ttgcgcccgt agctcgctga
    33301 cgctcacacg cgcagtacag cgccccgccc tgctgcaatg caccaggacg gcgtaaactc
    33361 gctggcgctc gcgctaactg cggcattctg cggcgcaaat cagcatgatc atggcctgtt
    33421 ggtctgttct ggctttgcag ggcgaaggga tggccctgtg catcctggta tgttcgtcag
    33481 cggctgcgcc gcgcttcggg gcgatg
//
LOCUS       DAZPBM010000028        32628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPBM010000028 DAZPBM010000000
VERSION     DAZPBM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.028"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            120..262
                     /locus_tag="ACHUZQ_001233"
                     /pseudo
     CDS             120..262
                     /locus_tag="ACHUZQ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="ACHUZQ_001234"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="ACHUZQ_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1024978.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="ACHUZQ_001235"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="ACHUZQ_001235"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1024979.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="ACHUZQ_001236"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="ACHUZQ_001236"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1024980.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="ACHUZQ_001237"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="ACHUZQ_001237"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1024981.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6652
                     /gene="hemX"
                     /locus_tag="ACHUZQ_001238"
     CDS             5417..6652
                     /gene="hemX"
                     /locus_tag="ACHUZQ_001238"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1024982.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQGDTPAAAPQGE"
     gene            6655..7851
                     /gene="hemY"
                     /locus_tag="ACHUZQ_001239"
     CDS             6655..7851
                     /gene="hemY"
                     /locus_tag="ACHUZQ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1024983.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8513..8650
                     /locus_tag="ACHUZQ_001240"
                     /pseudo
     CDS             8513..8650
                     /locus_tag="ACHUZQ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(8758..9993)
                     /locus_tag="ACHUZQ_001241"
     CDS             complement(8758..9993)
                     /locus_tag="ACHUZQ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1024984.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10140..10216)
                     /locus_tag="ACHUZQ_001242"
     tRNA            complement(10140..10216)
                     /locus_tag="ACHUZQ_001242"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10180..10182),aa:Pro,seq:tgg)
     gene            complement(10259..10345)
                     /locus_tag="ACHUZQ_001243"
     tRNA            complement(10259..10345)
                     /locus_tag="ACHUZQ_001243"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10309..10311),aa:Leu,seq:cag)
     gene            complement(10366..10441)
                     /locus_tag="ACHUZQ_001244"
     tRNA            complement(10366..10441)
                     /locus_tag="ACHUZQ_001244"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10406..10408),aa:His,seq:gtg)
     gene            complement(10500..10576)
                     /locus_tag="ACHUZQ_001245"
     tRNA            complement(10500..10576)
                     /locus_tag="ACHUZQ_001245"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10540..10542),aa:Arg,seq:ccg)
     gene            complement(10679..12064)
                     /locus_tag="ACHUZQ_001246"
     CDS             complement(10679..12064)
                     /locus_tag="ACHUZQ_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1024985.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12255..12995)
                     /gene="wecG"
                     /locus_tag="ACHUZQ_001247"
     CDS             complement(12255..12995)
                     /gene="wecG"
                     /locus_tag="ACHUZQ_001247"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1024986.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(12998..14350)
                     /gene="wzyE"
                     /locus_tag="ACHUZQ_001248"
     CDS             complement(12998..14350)
                     /gene="wzyE"
                     /locus_tag="ACHUZQ_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1024987.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14347..15426)
                     /gene="rffT"
                     /locus_tag="ACHUZQ_001249"
                     /gene_synonym="wecF"
     CDS             complement(14347..15426)
                     /gene="rffT"
                     /locus_tag="ACHUZQ_001249"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1024988.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15423..16673)
                     /gene="wzxE"
                     /locus_tag="ACHUZQ_001250"
     CDS             complement(15423..16673)
                     /gene="wzxE"
                     /locus_tag="ACHUZQ_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1024989.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16675..17805)
                     /gene="rffA"
                     /locus_tag="ACHUZQ_001251"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16675..17805)
                     /gene="rffA"
                     /locus_tag="ACHUZQ_001251"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1024990.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17810..18484)
                     /gene="rffC"
                     /locus_tag="ACHUZQ_001252"
                     /gene_synonym="wecD"
     CDS             complement(17810..18484)
                     /gene="rffC"
                     /locus_tag="ACHUZQ_001252"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1024991.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18462..19343)
                     /gene="rfbA"
                     /locus_tag="ACHUZQ_001253"
     CDS             complement(18462..19343)
                     /gene="rfbA"
                     /locus_tag="ACHUZQ_001253"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1024992.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19362..20429)
                     /gene="rffG"
                     /locus_tag="ACHUZQ_001254"
     CDS             complement(19362..20429)
                     /gene="rffG"
                     /locus_tag="ACHUZQ_001254"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1024993.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20429..21691)
                     /gene="wecC"
                     /locus_tag="ACHUZQ_001255"
     CDS             complement(20429..21691)
                     /gene="wecC"
                     /locus_tag="ACHUZQ_001255"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1024994.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21688..22818)
                     /gene="wecB"
                     /locus_tag="ACHUZQ_001256"
     CDS             complement(21688..22818)
                     /gene="wecB"
                     /locus_tag="ACHUZQ_001256"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1024995.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22874..23920)
                     /gene="wzzE"
                     /locus_tag="ACHUZQ_001257"
     CDS             complement(22874..23920)
                     /gene="wzzE"
                     /locus_tag="ACHUZQ_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1024996.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23932..25035)
                     /gene="wecA"
                     /locus_tag="ACHUZQ_001258"
     CDS             complement(23932..25035)
                     /gene="wecA"
                     /locus_tag="ACHUZQ_001258"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1024997.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25275..26534)
                     /gene="rho"
                     /locus_tag="ACHUZQ_001259"
     CDS             complement(25275..26534)
                     /gene="rho"
                     /locus_tag="ACHUZQ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1024998.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26619..26720)
                     /gene="rhoL"
                     /locus_tag="ACHUZQ_001260"
     CDS             complement(26619..26720)
                     /gene="rhoL"
                     /locus_tag="ACHUZQ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1024999.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26861..27190)
                     /gene="trxA"
                     /locus_tag="ACHUZQ_001261"
     CDS             complement(26861..27190)
                     /gene="trxA"
                     /locus_tag="ACHUZQ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1025000.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27321..28586
                     /gene="rhlB"
                     /locus_tag="ACHUZQ_001262"
     CDS             27321..28586
                     /gene="rhlB"
                     /locus_tag="ACHUZQ_001262"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1025001.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28720..30204
                     /gene="gppA"
                     /locus_tag="ACHUZQ_001263"
     CDS             28720..30204
                     /gene="gppA"
                     /locus_tag="ACHUZQ_001263"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1025002.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30251..32272)
                     /gene="rep"
                     /locus_tag="ACHUZQ_001264"
     CDS             complement(30251..32272)
                     /gene="rep"
                     /locus_tag="ACHUZQ_001264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1025003.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            32489..>32628
                     /locus_tag="ACHUZQ_001265"
     CDS             32489..>32628
                     /locus_tag="ACHUZQ_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025004.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         8228 a         8820 c         8185 g         7395 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg cacctgcacc
     6601 tgcacctgca ccgcaaggtg atactccggc agccgcgccg caaggagaat aaccatgcta
     6661 aaagtgttat tactctttgt tttgctgatt gcggggatag tggttggccc gatgattgcc
     6721 ggtcatcagg gttacgtgct gatccagact gacaactaca atatcgaaac cagcgtcacg
     6781 ggcctggcga ttattttgat tctggcgatg gtagtgctgt ttgccattga gtggctactg
     6841 cggcggatct tccgcacagg cgcacacacc cgtgggtggt ttgtcggacg taagcgtcgc
     6901 cgtgcccgta agcagaccga acaggcgctg ctgaaactgg cggaaggcga ttatcagcaa
     6961 gttgaaaagc tgatggcgaa aaatgccgat cacgcggaac aaccggtggt gaactatcta
     7021 ctggctgccg aagccgcgca acaacgtggt gatgaagcac gcgccaacca acatctggaa
     7081 cgcgcagcag agctggccgg caatgacacc attccggtag aaatcacccg tgtacgtctg
     7141 caactggccc gtaatgaaaa ccatgctgca cgccacggcg tggataagct gctggaagtt
     7201 acgccacgcc atccggaagt attacgtctg gcggaacagg cgtatatccg cacaggtgca
     7261 tggagttctc tgctggatat catcccatca atggcgaaag cccatgttgg tgatgaagaa
     7321 catcgtgcaa tgctggaaca acaggcatgg attggcctga tggatcaggc gcgtgccgat
     7381 aacggtagtg aaggtttgcg taactggtgg aaaaaccaaa gccggaaaac gcgtcatcag
     7441 gtagcgttgc aggtggcaat ggcggaacat cttattgaat gtgacgatca tgatactgcc
     7501 cagcaaatta tcatcgatgg cctgaaacgc cagtacgacg atcgcctact gctgccgatt
     7561 cctcgactga aaaccaacaa tccggaacag ctggaaaaag tgctgcgcca gcagatcaaa
     7621 aacgtcggcg atcgcccgct gttgtggagc acactgggcc agtcactgat gaagcacgga
     7681 gaatggcagg aagcatcgct cgccttccgc gcagcgctga aacaacgtcc ggacgcctac
     7741 gattacgcat ggcttgccga cgcgctggac agactgcaca agccggaaga agctgcagcg
     7801 atgcgtcgcg acggtttgat gttaacgttg cagaacaacc caccacagta gttccttctc
     7861 acccggaggc aagcacctcc gggacctttc tgatgcataa aaaaacgcct gctcttatta
     7921 cggagcaggc gttaaaacag gtctgtatga caacagtggg tgcttcactc aacgttgtgt
     7981 ccatggtgtc tgatgaggca taagcgacat ctgtcagtgg acgataagca ccgtaaacgg
     8041 ctctgcgtca ttccggagtt tatgaggcac taaggcgaac ataagagatg gaatgagcat
     8101 ctacccgttt attatgccac agagaatcgg gaaataacat cccttaacac ttgttatgag
     8161 ataattctgt aatcctcttt gcttcctgag tgaatattta acctgagctt gatcctacac
     8221 atacttatta tgaatgataa aattcattca attaataaca catatattaa ttgccgttaa
     8281 aactaaaaaa gcattaataa tcaacgcaat ataataaacc cgccttacat atcaactgcg
     8341 ccagaggtag gattgaaaac gctctcctga ttttccaatt cattttctgg ataaataaat
     8401 aatttatttt tgtcactatt atttatgtaa tcatcctgtc agggagaggg atctcaatta
     8461 tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt gcatggaatt
     8521 ttcgttttca cccaaacttc ttgttgttgc tgtcgccgcc gctcttcctc tcatggccag
     8581 cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc tttgccggat acgatcaccc
     8641 aaaccagtat ccacagaacg ctgcaattta ttgaatttgc acgatcatgt aggccggata
     8701 aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc acaaaatggc cgctttttta
     8761 cttattcacc accagcaaat gtgcctgcat aatgtcactg gccgggcgac cgtgcgccag
     8821 caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
     8881 acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt gtttcgggca
     8941 gcctccccag cacgctttta acacgttaca actgcgacac tgcgccggta attgcttaaa
     9001 tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa ttgtttgctg
     9061 atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt aaacgtcgcc
     9121 gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcacccgg
     9181 cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct tgccgacgtc
     9241 gcgtttgatc cagtggtcga atatcgccac cagaaattca ccaaattcat cggggcgcac
     9301 cgaccattcc gtcagttccc cctgaatatc accaggcgca tgcaatttca gtccatcgcg
     9361 ggcagctgtt tcatcagcca gacgctcgac caccggaata aactggatga attcgactcc
     9421 cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc gcgctggtgc ggttgacgca
     9481 gaccagcacg ttatagtcga catgatgttt ttgcagcagc gtcagggcac gcatcaccag
     9541 cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct cagccggacc
     9601 gtccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc atttatcatc
     9661 gagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac cgtatttcgc
     9721 ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt aacgttggtt cgccgccctg
     9781 ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac ggacatacgc
     9841 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg attgtttttc
     9901 gagataaaaa cagtagtcac agttcagatt acaatcggaa ccactcggtt tcgccatcac
     9961 atgaaaagca cgcgttggaa cctgttgcag catggtcact ccttgcctga agtcatcctt
    10021 caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc gtttattgcg
    10081 aagggagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg gttcaaaatt
    10141 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
    10201 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt taaagtcgtg
    10261 gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
    10321 cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg tggctaatgg
    10381 gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga gctatagcca
    10441 ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc gggactaaat
    10501 ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc gctctatcca
    10561 gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt cccacccctc
    10621 tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt tttgctgctt
    10681 attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta taaatcgccg
    10741 tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct tcattaaagt
    10801 acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc cacgggaaca
    10861 ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga aaacgcagct
    10921 ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca ctgtagacgt
    10981 agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca attagcagaa
    11041 tagcaataga tactgccaca cccgcaactg gtacgccgtg acgggaaact ttcgccatcg
    11101 ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa ctgtacatgc
    11161 cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg cccgccgctg
    11221 cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg ccaatttcat
    11281 tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc aggatccgcc
    11341 acagcacttt gcctacagca ctgcgcagcg tcacctgcgg attcttcgct tcaccggcag
    11401 taatgccaat cagctccacg ccctggtagg acgccaccac aatacacagc gcggtcagga
    11461 accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta aaaccaatcg
    11521 actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca atcatcacga
    11581 taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac aaccgcaccg
    11641 ccgccagatt cgccaacgcc accagcgcca ccgcgatcaa tgcaggtatc cactgcgcca
    11701 tctccgggaa ccagaactgg acataaacgc caatggcggt gatttctgag atccccaccg
    11761 ccatccacat aaaccagtaa gaccaggcgg tgagataacc aaaaaacggg ctcatataac
    11821 gatgcgcata aacggcgaac gaaccggtaa caggttcaag gaacaacatt tcgcccattg
    11881 aacgcatgat gaaaaagacg aacagcccgg cgatgatata ggccaacaat acggatggcc
    11941 cggcccattt cagggtactg gcggccccca taaacaggcc gacgccaata gtgcccccca
    12001 gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt ttgttatctg
    12061 ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc gttatgggta
    12121 catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag cattaaaaat
    12181 ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc cgtcgcttta
    12241 caaagagagg aaaatcagag gttgccggtg tagtgccagc gtaaataacg cagcaaacga
    12301 agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc cagtcccagc
    12361 gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta agtcccgcca
    12421 acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa gatctcctgc
    12481 tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac
    12541 agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat attcacattc
    12601 cattggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc gcccacaaga
    12661 aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag atcggcaccg
    12721 gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac aacgctgatg
    12781 ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcggc gttatcttca
    12841 atggtcagca ttttttcagc attaatggca accagcgttc cctgcttaag ctgcccgtca
    12901 gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg taagccacgc
    12961 agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga
    13021 gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag tacagcagtt
    13081 ttgcgattat cagacatgcg ccgaagacca cgataaaaaa gactacgcgt gagacaaacg
    13141 aatccagccc ttcacgcgcc agcacgatca tattgaagat cgccccaaag cagaaactgt
    13201 gcaatatcgc agccttatag cgattaggct cgcggttgcc cagctcatac agccagtcga
    13261 accatttgat gatcagacca accacgatcg ccccgagcgg gatgaacaac gcgccgccca
    13321 tcaccaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg ttattcagca
    13381 cttcccaggt aaagtagttg gctgagttca gcaccatact cgggcgaccc ggccacagcc
    13441 aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg aagtcgatgt
    13501 tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg ggagaaggtg tcgcgagtga
    13561 gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag cgttttagcg
    13621 ccatccagaa catgccaaca atccccagca cgcccgccgc cgccagcatc cacaacgaaa
    13681 tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg atattggcgc
    13741 gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg acgaggaaaa
    13801 acagccacgc tttgctgtcc tggcgcagaa agtagaccac cagcatcgcc gggatgaaaa
    13861 agtaaaagaa gcgttttaac gccacgccgg agacttcact ggagaagatc tgactgtagg
    13921 agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg cttaccagcg
    13981 cgatacccat caggatcacc cacgtaagat tggtctccac gcggttcatg gtaaacagcg
    14041 gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg acatagtaaa
    14101 ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac
    14161 caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa gccgaagaaa aaggtgagca
    14221 aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac tcaaaccagg
    14281 tcagcgtggc aataaacagc gtgcagagca gccagaccac aaacaggcca ctgaattgca
    14341 gcagactcat gcgacctccc cggcggcaat cgccaacgcc cgctgccagc cttgtagata
    14401 gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc gctgcgcttc
    14461 acgcacaata tcctcgttga gatcgtcggt agtaaacagc accggcaaat gctgttccgt
    14521 catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg cctgaatcag
    14581 taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccaa gatcgcactg
    14641 acgaagtagc gccagatagg cgtcaaattc cagtttttcg ctcaaaattt gcagattttc
    14701 ttcgctgaat aactccagcc ccgcctgacg aacttcctca atgtacgctt cgttattagg
    14761 cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat gaacggcgcg
    14821 caaggcagca atatggtcat tgctgcggtc accggagttc cccaccagaa tggtcatttt
    14881 cccttcacgt tgccgatcat tcgccatcgt attgagagaa gggtccatcc gcgtcgggaa
    14941 gtacagcagt tcgccccgca cctttggatg cgttttggca aaaaagctca aatcaccgcg
    15001 ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagcgggt aaaaaagctt
    15061 atatctcaag ccactggaaa gctcatacag gtctgccccc cagatatgcc agaaaaactg
    15121 gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga actgaccgtg
    15181 gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg atgaccgctt ccgccagcga
    15241 ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta agccgtcgtc
    15301 cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcggccagcg cgtcattgaa
    15361 aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt gaatcagtac
    15421 agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa atagacgata
    15481 taagttgcca tatatgcctg cgccgcgccc agtgcaccat gcgcagggat tagccagtgg
    15541 gcaaatacca tcaataaagt gaactggctg acttccgcca gaatataaaa ccgcagtgac
    15601 gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac atcacccact
    15661 aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag caaccagata
    15721 gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg ccactgccgg taagacgaat
    15781 ttcagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg cgacaacgtg
    15841 ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc ggaaatactg
    15901 ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgccag cagtttacgc
    15961 atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag cgtaaatttg
    16021 ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa cgggatgaca
    16081 ccacgtttga tcaacatgat ggcggcagga atcaccacca gcgcgggaat cagcgccaga
    16141 cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc gagaacgcca
    16201 atcaagctgc cgacaatcag cgataacgca ttacctgcgg catcgcgaaa gcctttcatc
    16261 agcgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac taaacgcacc
    16321 agcccctgat agtcggtatt accaaacagt ccctggctga ttggcgcagc tgccagcaca
    16381 aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt gccgaccacg
    16441 cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac accgttaaag
    16501 atgccagctc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt tgccgccagc
    16561 ccaagccccg ccggaccaaa tgacacagcc agcaacttac cgaccagtaa cccggcacca
    16621 atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga catatcagga
    16681 gaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca ggttgtagaa
    16741 cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt caccgtggaa
    16801 ctcaccaaag cgttcccccg cagggcaacc gtgcagcgga atgtaatgga acaccgccat
    16861 gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat cccgcagttt
    16921 aatgtagaac atatgcgcgt tctgcacgca gccatcggga atcgacggca gctcgatacg
    16981 cccggctttc gccagaggcg ctaacgcatc gtagtagttt tgccacagcg ccagacgttg
    17041 ctggttgata cgatccgctg cttccagttg cgcccacagg tacgcagctt gcagatcgga
    17101 catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac cacggaagaa
    17161 ctggctgcgg ttagtgccct tttcacggat gatctcggct cgttcgatta acgctttatc
    17221 gttaatcagc gtcgcgccgc cttcaccacc cgccgtatag tttttggttt catggaagct
    17281 aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg acatcacgcc
    17341 ctgagcggca tcttctacca caaacagatt atgctttttc gccaacgcca taatggtgtc
    17401 catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt tgtcggtgat
    17461 cgccgcttca atcagcgttt cgtcgatgtt catggtgtcc gggcgaacat ccacaaaaac
    17521 gatttttgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt agctcggcat
    17581 gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca gcgaagcggt
    17641 gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc actgctggca
    17701 gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg actgcatata
    17761 gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat cacctgtata
    17821 accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta agcgcggcgg
    17881 tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac gcccagttta
    17941 gcgccgtttg catcagctca gcacctgcac cgcgtccagc cagcaggcca attcgcgcat
    18001 ctgtcgcatt gagttcccgt aaagagacat aaccgcgaat accgccggac gccgcacgta
    18061 aaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa
    18121 agcgtccgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg
    18181 cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactaca gcaccactat
    18241 cactgacatt gttcacgggt agtgccaaat caacttcacc ttctaccagg gagaatccca
    18301 gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc tgcacccgtg
    18361 accacggcgc taacacgtct ggcgtcagga gcggtgcttc agacgtaatg cgcacgatgg
    18421 cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg gcgcggacgg
    18481 gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga actggcagca
    18541 cgcttcacac cctcgtcatc gagccagccg ttacgccagg cgatctcttc caggcaggca
    18601 atcttaaagc cctggcgttt ttctactgtc tgcacaaagg tactggcttc aatcaggctg
    18661 tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg
    18721 cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg ctccgacggc
    18781 ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc
    18841 cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa attgtcgtca
    18901 aactccacca cgccaaagcg ttccgggtcc atcacctgat agccaaatac cgtcgcccct
    18961 tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa gaagatgtta
    19021 tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat gataaaggcc
    19081 tgcgccagac cgtccgggct gggctgttcg gcgtattcca gctggatacc gaactcacta
    19141 ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat gatgagaatt
    19201 tcgcggatat cggccaacat cagcaccgac agcggatagt aaatcattgg cttatcgtaa
    19261 atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg
    19321 cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt cagacctaaa
    19381 cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt agccagatac
    19441 cactgcaccg ttttacgcat tccactttca aaggtttcct gtggcagcca gccaagttca
    19501 cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg gtcagcgaca
    19561 aaggtgatca agtcacgata gtgcaccacg ccgtgcggct tgttcggagc cagttcttcc
    19621 agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg accaccaata
    19681 ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg ggcgtgatct
    19741 tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag cggtttaccc
    19801 gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta aggaccgtag
    19861 ttattcgagc agttggtgat aagcgtcggc agaccgtagg tccgcagcca ggcgcgcacc
    19921 agatggtcgc tgctggcttt tgacgcggaa taagggctgc tcggcgcata cggcgtggtt
    19981 tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt ggagatatga
    20041 tgaaaacgga acgctgattt tttatcttcc gtcagcgtat tccagtaagc ccgcgccgct
    20101 tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg gccgtcaata
    20161 gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg atgctcagtg
    20221 aatacgcgtg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc aaagcgctcg
    20281 ctttgcgcga ccggtgccag cgacatcagg tttccggcgt aggtcagctt atcgaccact
    20341 accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc aataaacccg
    20401 gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca tcgacgacat
    20461 actgctgatg gacattgtcg ccattgataa ctttaaactg actatgatcg accagcatca
    20521 ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa agcccggtca
    20581 gttttttcgg caactggtgg atgttaggct caacaaccag agtttcgccg ctatgccact
    20641 gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca atattcggtt
    20701 taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg gtagccgcca
    20761 ggcaatcagc caccgccgct ttcacctgat cgataaccca gaacggtttg tgatcgttca
    20821 cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg ataaaccacg
    20881 gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata ttaacgcgag
    20941 ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg gcacaaatca
    21001 gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg gtgagcttac
    21061 acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa attttgtaca
    21121 gttcgctggc gcgggtcgaa caaaccggcg tcataccacc aatcacgcga tcgtttttaa
    21181 tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca atgttgacgt
    21241 ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg acgcatctct gctaaccatt
    21301 ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc agcgcgcctt
    21361 ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata tctggctcat
    21421 gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca actggcgtcg
    21481 tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc aaatcaggct
    21541 cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg atatcgacac
    21601 caatcacctg tttttgccgt gaggcaaacg ctgctgctgt aggcagcccg atgtaaccca
    21661 gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt ttaacgcttc
    21721 cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc tcatagtttg
    21781 atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct gcttatccgt
    21841 gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg tatcgcgcat
    21901 caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa
    21961 aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctggg gatcgatcag
    22021 aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt tcgggttgag
    22081 atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcaatgtctg ccaacgcgtg
    22141 gcagatttct tcaaagccac gaccgaaact ttcacgcctg tgaccggtca ccagaatcat
    22201 ctttttatcg gggtcgataa acgggtaatt tgccgccagt tctgaacgca gcgtatcgct
    22261 gctcatcacc tggtcacgca cccataacag tgcatcaata actgtattac cagtaatgaa
    22321 gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt cggttggaga
    22381 gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg gccacggcga
    22441 ataaagatcg cccgtgcgca gaccagcctc aacgtgacca acaggaatac gctgataaaa
    22501 cgccgccagg ctggttgcca gcgtcgtcgt tgtgtcgccg tgaaccagca cgacgtctgg
    22561 tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta tctctgtcag
    22621 gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa agagtttcag
    22681 cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct caaaaaaagg
    22741 atcttttgcc aacgcatgca ccaacggcgc catcttgatg gcttccgggc gcgtaccaaa
    22801 tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct ttgcgctcac
    22861 cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc ccgatcagcc
    22921 ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg cgttttaccg
    22981 gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccagg gttggaccaa
    23041 cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag gccggaccga
    23101 cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg aacatttctg
    23161 aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta tgctgctcag
    23221 caattttcag cgcctgttca atactgttca tccggcggtc gtagatggct ttcgccacct
    23281 cttcctgacg cttcacctgt gctttcatct ggatggtacg cgccgcccat gcgcctttca
    23341 gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac tgacgtaaca
    23401 ggttattagc gtcaggcgcg gtttcagcaa taagcttcac gctgtcattg accgcgcggg
    23461 taaagtctcc ggggataaac tggatgttgt taatcatttc atccagcaac gccgcatcgg
    23521 ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt ttgcagccag aactctctgc
    23581 gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc atgaccgatg
    23641 gttggtcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat tgctgctgcg
    23701 agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgccgtc gagctccact
    23761 cctgacgagc aaaaaaagta tacgccagcg cgattaacgc aaatgccagc cccatgccaa
    23821 taatccatag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc agttcatttt
    23881 cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca tttatttggt
    23941 taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct taataaagcg
    24001 agcaactttc caggcacgct taatgcaata tccatagagg aagaatgcta gcaaaaagag
    24061 caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc caatggaagc
    24121 gagcagtgcc gcagcaaggg taatcagcac aaacgcctga cgggaagtaa acccggcacg
    24181 catgatcaaa tggtgaatat gctgacggtc aggagagaat gggctcatgc ctttacgcag
    24241 gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc acaaagcggt
    24301 aaccgggctg atgggatggg ttttgccctg ggtcgtttcg agcaggatcc agataacggt
    24361 aaaaccaatc agcgtactgc ccgcatcacc cataaagact ttgtagcggc gacccaggat
    24421 accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc accagattgc
    24481 gaggctggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg agacgcagga
    24541 caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa tggccgccca
    24601 gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc aggagccaaa
    24661 gatataaccc aggctactga gataaagttt gccgaacacc atcataacaa tgccaacagc
    24721 ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca gcgcgccaat
    24781 gaaaacaagc acaccggcac aagcgagata gagagatgca tgcggaatat agtaatcgac
    24841 aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga gaggtatcaa
    24901 tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt ttgccacctt
    24961 acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac
    25021 tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt ataaccacga
    25081 agacctttat tcagaaggga aagtccgttc cgaacccacc agtcctgtgc aaaaattaac
    25141 catttgatat gcaaattgca taacacgcca gcaaattcca ggaattttcc taacatcgcc
    25201 agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg tggcgttttt
    25261 ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc
    25321 atggccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg gtgaatgatt
    25381 ttgcgcagga tccacatttt ctgcagttct tcctgagtag tgagcagttc ttctttacgg
    25441 gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat cttacgagag
    25501 aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt agataacttc gtccatttta
    25561 gaaccggtat cgataagcgc cgtcgcgata atagtcaggc tgccgccctc ttccacgtta
    25621 cgcgccgcac caaagaagcg tttcggacga tgcagggcgt tggcatccac accaccggtc
    25681 aacactttac ctgacgccgg aacaacggtg ttgtaagcgc gcgccagacg agtgatggag
    25741 tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttggcctt ctcgatcacc
    25801 atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc aacaacttca
    25861 ccttttacca gacgctgcat ctcggttact tcttccggac gttcgtcgat cagcagaacc
    25921 atcagcacac aatccgggtg gttgtaagca atgctctggg caatgttctg cagcagcatg
    25981 gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc gataggtgat
    26041 gccagatcca gtacgcgagc agttaaatct tcagtagaac cgttaccacg ttccatacgc
    26101 agacgagaat ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt gcgggcgttt
    26161 tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg ttcaccttct
    26221 ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa acggcggatt
    26281 tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc tgcggaacgg
    26341 aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat atcttcgcca
    26401 ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat gcgagccagg
    26461 ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt attctttaat
    26521 tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga acttaatgtt
    26581 gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat gtcttttcgc
    26641 tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag tgacgagcca
    26701 gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta caagattcaa
    26761 acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc acgccgggcg
    26821 tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg ttagcgtcga
    26881 ggaactcttt caactgacct ttagacagtg cgcccacttt ggttgccgcc acttcaccgt
    26941 ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca gtgccagggt
    27001 tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca gcgatttcat
    27061 ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa tcgacgagga
    27121 tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg tgaataattt
    27181 tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt tgttggtgta
    27241 acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa agcaatagta
    27301 agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag ttttccgact
    27361 tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa agggtttcat aactgtacgc
    27421 ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc
    27481 aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat cttctctctc
    27541 atcctgcgat tgccgatcgc aaggtgaatc aaccgcgtgc cttaattatg gcaccgacgc
    27601 gtgaacttgc cgtgcagatc catgccgacg cagaaccgct ggcggaagct actggcctga
    27661 agctgggtct ggcttacggt ggtgatggct acgacaaaca gctgaaagtg ctggaaagcg
    27721 gcgttgacat tctgattggc accacgggtc gtttaattga ctacgccaag cagaaccaca
    27781 ttaacctcgg tgccattcag gtagtggttc tggatgaagc cgatcgcatg tacgatctgg
    27841 gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac cagcgcctca
    27901 acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc gagcagatga
    27961 acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt attaaagaag
    28021 agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag
    28081 agtggccaga cagagcgatt attttcgcca ataccaaaca ccgttgtgaa gagatctggg
    28141 gccacctggc ggcagatggt catcgtgtcg gtttgttgac aggcgatgtc gcgcagaaaa
    28201 aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg gttgccaccg
    28261 acgttgccgc gcgtggtttg catattccgg cagtgacgca cgtctttaac tacgatttac
    28321 ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca ggcgcaagcg
    28381 gtcactctat cagcctggcg tgtgaagagt atgcattgaa tttgcctgct attgaaacct
    28441 atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg accgatctgc
    28501 caaaaccgct gcgcctcacg cgcccacgta caggcaatgg tccgcgtcgt actggcgctc
    28561 cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac tcaaaatcat tcaaactgta
    28621 tcaagatggc aagagaatga atcccgatga gcatactgta gtaagtgatt caggtgaaag
    28681 aatgcagcca acacagagac agattgaagg atgaagagta tgggttccac ctcgtcgctg
    28741 tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc tggttgtgcg cgaggtggct
    28801 ggaagcatcc agacgctgac gcgaataaaa cgcaaagtgc gtctggctgc tggcctgaat
    28861 agcgaaaatg ccctgtctaa tgaagcaatg gagcgcggtt ggcaatgtct gcgcctgttt
    28921 gctgaacgtc tgcaagatat ccctccctcg caaattcgcg ttgtcgctac ggcgacatta
    28981 cgtcttgccg tcaatgcggg tgattttatt gccaatgcgc aggaaatcct cggttgtccg
    29041 gtacaggtga tcagcggtga agaggaagca cgtctgattt atcagggcgt tgctcacaca
    29101 actggcggtg ccgatcagcg cctggtggtg gatataggcg gtgccagtac tgaactggta
    29161 accggcacgg gtgcacaaac cacctcgttg ttcagcctgt cgatgggctg cgtcacctgg
    29221 ctggaacgct attttgccga tcgtaatctg gggcaggaaa attttgatgc tgcagaaaaa
    29281 gcggcacgcg aagtgttacg tccggttgcc gatgaattac ggtatcacgg ctggaaagtg
    29341 tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa tcatgatggc acaggggatg
    29401 gatgaacgca ttaccctgga aaagttgcag caattgaaac agcgagccat tcattgcggt
    29461 cggctggagg aactggagat tgacgggctg acgctggaac gtgcgttagt gttcccgagt
    29521 ggtctggcga tcctgatcgc catttttacc gaactgaata ttcagtgtat gaccctggcg
    29581 ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc atctggccgt cgagcaggat
    29641 attcgcagcc gtacgctgcg taatattcag cgccgcttta tgatcgatat tgatcaggcg
    29701 cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg tggaaaagga atggcatctt
    29761 gaagcaataa gccgcgattt gctcatcagc gcctgtcagc ttcatgaaat cggcctgagc
    29821 gttgacttta aacaagcgcc gcaacacgct gcttatctgg tgcgtaatct ggatcttccc
    29881 ggttttaccc ccgcacagaa aaaattgctg gcgacgctac tgctcaacca gactaatccg
    29941 gtcgatctct catcgctgca tcagcaaaat gccgtaccgc cgcgcgtcgc agaacaactc
    30001 tgccgtttac tccgcctggc catcattttt gccagccgtc gccgtgacga tctcgtgcca
    30061 gagatgacat tacaggctaa ccatgaactg ttgaccttga cgcttccgca aggttggcta
    30121 acccaacatc cgctgggtaa agagattatt gctcaggaaa gccagtggca gagctatgtc
    30181 cactggccgc tggaagtgca ttaatgagta agtgccggat gcgatgctga cgcatctttt
    30241 ccggccttga ttatttccct cgttttgccg ccatcatcgc tttcagattc gccagatggc
    30301 tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt gcgctcctgt tcccaaatca
    30361 gatcatcctg cggcagctcc agcaaaaagc ggctcggctc cgggcgcacc agctcgccgt
    30421 actgacggcg ttctttacac agcgtaaagg tcaattcctt ctgggcgcgg gtaatgccga
    30481 cataggccag ccgccgctcc tcatcgatat tatcttcatc gatgctgctc tggtgcggca
    30541 aaaacccttc ttccataccg accatgtaga cataaggaaa ctccagccct tttgacgcgt
    30601 gcagagtcat cagttgcacc tgatccagct cttcttcact ctcaccacgc tccatcatgt
    30661 cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat cggctcatcc agttcgctgc
    30721 cttccagcat ctccgtcatc cagctaaaca gttggttgac gttcttcatg cgcatttcgg
    30781 cggctttcgg gctgggcgat gtttcgtaca gccaggattc ataatccatg ccgtggatca
    30841 gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg ctggatttct gccaaccagt
    30901 gagtaaagcg ggtcaatgct tcataaccac gtccgctaag cgtctgactc aggcccatat
    30961 caaagctggc ggtaaacatg cttttattgc gcgtcatcgc ccactcaccc agctttttca
    31021 gcgtcgccgg gccaatctct ctcttcggcg tgttaacgat acgcagaaat gcgctgtcat
    31081 cgtccgggtt agtcagcacg cgcagataag ccagcaagtc cttgatttca ggacgagaga
    31141 aaaacgacgt accaccagaa attttgtacg ggatgcggtt ttgcatcagg aatttttcaa
    31201 acactcgcga ctgatggtta ccgcgataaa gaatagcgta atctttgtac tgcgttttat
    31261 tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc ggcttcatgt tcttcgttat
    31321 tcgcgcttaa aactttaagc tctgtgccgt aacccagttc ggagaacaga cgcttctcaa
    31381 agacgtgcgg gttattggcg atcaggatgt tcgccgcttt caaaatacgt ccggaagagc
    31441 ggtagttctg ctcaagctta atcaccttca gcgccggaaa atcctgactc agcagcacca
    31501 ggttttgtgg acgtgcgccg cgccaggagt agatcgactg gtcgtcatcg cccaccacgg
    31561 taaagcgcgc gcggctgccc accagcagtt tcaccagttc atactggctg gtgttggtgt
    31621 cctgatactc atccaccagc agatagcgaa ttttgttctg ccagcgctcg cggacttctt
    31681 cattgcgttg cagcagcaac gtcggcaata aaatcagatc atcgaagtcg agaacattgc
    31741 acgctttcag gtgtgcatca tacagcccat aacaatgggc aaaaatacgg tcccgctcgc
    31801 caatcgcact ggctgccgcc tgggacggtg ttttgagatc gttcttccag ttagagatgg
    31861 tcgaaatcaa ctgttgcagg agaactttgt catcttcaat cagcccttcg gtcaactctt
    31921 tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa gttcgccttc atccccagcg
    31981 ccgcatactc acgcttgatg atatccagcc ccagcgtgtg gaaggttgag atcatcagcc
    32041 cacgcgcctc tttgcgaccc agcgtctgtc cgacacgctc tttcatctcg cgcgccgcct
    32101 tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata accgcaaccg cggatcagat
    32161 gggcgatttt attggtgatc acacgagttt taccggaacc cgcgcccgcc agcaccaggc
    32221 aggggccggt aacgaattcg acagcttgtt gttggccggg gtttagacgc ataggtgtat
    32281 tgctcaatct tcgaacgggg ggaggattgt agcagaatcg catttgaatg tgaccgcgtg
    32341 taagccgcta accgattggc tgcggatgtt taaatcagag agggaagacc gaaaccggag
    32401 ttcatcgtga atccattaga cttagaaaat atcgggtcgt aacgttgtgt tattactttt
    32461 tcagagttta ctaaggagag gtgtactcgt ggcgattaat ttcagtccta aggttggtga
    32521 aatactggat tgcaactttg gtaactatcc tgttagccag aatggacagt tcagcaccac
    32581 gtattacgat ggccgcattc ctcctgaaat gataaaaaac aggttagt
//
LOCUS       DAZPBM010000029        31117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPBM010000029 DAZPBM010000000
VERSION     DAZPBM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.029"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            19..714
                     /gene="araD"
                     /locus_tag="ACHUZQ_001266"
     CDS             19..714
                     /gene="araD"
                     /locus_tag="ACHUZQ_001266"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1025005.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            789..3140
                     /gene="polB"
                     /locus_tag="ACHUZQ_001267"
     CDS             789..3140
                     /gene="polB"
                     /locus_tag="ACHUZQ_001267"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1025006.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3304..6210
                     /gene="rapA"
                     /locus_tag="ACHUZQ_001268"
     CDS             3304..6210
                     /gene="rapA"
                     /locus_tag="ACHUZQ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1025007.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6222..6881
                     /gene="rluA"
                     /locus_tag="ACHUZQ_001269"
     CDS             6222..6881
                     /gene="rluA"
                     /locus_tag="ACHUZQ_001269"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1025008.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHUZQ_001270"
     CDS             complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHUZQ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1025009.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8068..10422
                     /gene="lptD"
                     /locus_tag="ACHUZQ_001271"
     CDS             8068..10422
                     /gene="lptD"
                     /locus_tag="ACHUZQ_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1025010.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10475..11761
                     /gene="surA"
                     /locus_tag="ACHUZQ_001272"
     CDS             10475..11761
                     /gene="surA"
                     /locus_tag="ACHUZQ_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1025011.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHUZQ_001273"
     CDS             11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHUZQ_001273"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1025012.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHUZQ_001274"
     CDS             12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHUZQ_001274"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1025013.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13571..13948
                     /gene="apaG"
                     /locus_tag="ACHUZQ_001275"
     CDS             13571..13948
                     /gene="apaG"
                     /locus_tag="ACHUZQ_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1025014.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13955..14797
                     /gene="apaH"
                     /locus_tag="ACHUZQ_001276"
     CDS             13955..14797
                     /gene="apaH"
                     /locus_tag="ACHUZQ_001276"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1025015.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHUZQ_001277"
     CDS             complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHUZQ_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1025016.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHUZQ_001278"
     CDS             complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHUZQ_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR1025017.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHUZQ_001279"
     CDS             complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHUZQ_001279"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1025018.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18039..19370)
                     /locus_tag="ACHUZQ_001280"
                     /pseudo
     CDS             complement(18039..19370)
                     /locus_tag="ACHUZQ_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHUZQ_001281"
     CDS             complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHUZQ_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1025019.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(19713..20999)
                     /locus_tag="ACHUZQ_001282"
     CDS             complement(19713..20999)
                     /locus_tag="ACHUZQ_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1025020.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMNYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21050..21991)
                     /locus_tag="ACHUZQ_001283"
     CDS             complement(21050..21991)
                     /locus_tag="ACHUZQ_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1025021.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22006..22776)
                     /locus_tag="ACHUZQ_001284"
     CDS             complement(22006..22776)
                     /locus_tag="ACHUZQ_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1025022.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            23249..24763
                     /gene="caiT"
                     /locus_tag="ACHUZQ_001285"
     CDS             23249..24763
                     /gene="caiT"
                     /locus_tag="ACHUZQ_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR1025023.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24794..25936
                     /gene="caiA"
                     /locus_tag="ACHUZQ_001286"
     CDS             24794..25936
                     /gene="caiA"
                     /locus_tag="ACHUZQ_001286"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1025024.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            26065..27282
                     /gene="caiB"
                     /locus_tag="ACHUZQ_001287"
                     /pseudo
     CDS             26065..27282
                     /gene="caiB"
                     /locus_tag="ACHUZQ_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            27356..28909
                     /gene="caiC"
                     /locus_tag="ACHUZQ_001288"
     CDS             27356..28909
                     /gene="caiC"
                     /locus_tag="ACHUZQ_001288"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1025025.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            29018..29803
                     /gene="caiD"
                     /locus_tag="ACHUZQ_001289"
     CDS             29018..29803
                     /gene="caiD"
                     /locus_tag="ACHUZQ_001289"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1025026.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            29809..30399
                     /gene="caiE"
                     /locus_tag="ACHUZQ_001290"
     CDS             29809..30399
                     /gene="caiE"
                     /locus_tag="ACHUZQ_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1025027.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="ACHUZQ_001291"
     CDS             complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="ACHUZQ_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1025028.1"
                     /translation="MCEGYVEKPPYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT         7541 a         8375 c         8150 g         7051 t
ORIGIN      
        1 gacctctcag gagtcaacat gttagaagat ctcaaacgcc aggtattaga agccaacctg
       61 gcgctgccaa aacacaacct ggtcacgctc acatggggca acgtcagcgc cgttgatcgc
      121 gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac
      181 gatatggtcg tggttagcat cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc
      241 tccgacacgc caactcaccg gctgctctat caggcattcc cctccattgg cggcattgtg
      301 catacgcact cgcgccacgc caccatctgg gcgcagtcgg gtcagtcgat tccagcaacc
      361 ggcaccaccc acgccgacta tttctacggc accattccct gcacccgcaa aatgaccgac
      421 gcagaaatca acggcgaata tgagtgggaa accggtaacg tcatcgtaga aacctttgaa
      481 aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg tccattccca cggcccgttt
      541 gcatggggca aaaatgccga agatgcggtg cataacgcca tcgtgctgga agaggtcgct
      601 tatatgggga tattctgccg tcagttagcg ccgcagttac cggatatgca gcaaacgctg
      661 ctggataaac actatctgcg taagcatggc gcgaaggcat attacgggca gtaatgactg
      721 tataaaacca cagccaatca aacgaaacca ggctatactc aagcctggtt ttttgatgga
      781 ttttcagcgt ggcgcaggca ggttttatct taacccgaca ctggcgggac accccgcaag
      841 ggacagaagt ctccttctgg ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac
      901 cgcaagagtc cgtggcgttt attcccgccg atcaggttcc ccgcgctcag catattttgc
      961 agggtaaaca aggctttcgc ctgacaccgc tggcgttaaa ggattttcac cgccagccgg
     1021 tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg
     1081 aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc agaacgctat ctgatggagc
     1141 ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca caatggcact atcgttaatg
     1201 cccgtctgaa accgcatccc gactatcgtc cgccgctcaa gtgggtttct atagatattg
     1261 aaaccacccg ccacggtgag ctgtactgca tcggcctgga aggctgcggg cagcgcatcg
     1321 tttatatgct ggggccggag aatggcgacg cctcctcgct tgatttcgaa ctggaatacg
     1381 tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg gtttgccaac tacgatcctg
     1441 atgtgatcat cggttggaac gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg
     1501 agcgttaccg tcttccgctg cgtcttgggc gcgataatag cgagctggag tggcgcgagc
     1561 acggctttaa aaacggcgtc ttttttgccc aggctaaagg tcggctaatt atcgacggta
     1621 tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt ctcgctggaa actgtcgctc
     1681 aggagctatt aggcgaagga aaatctatcg ataacccgtg ggatcgaatg gacgaaattg
     1741 accgccgttt cgccgaagat aaacctgcgc tggcaactta taacctgaaa gattgcgagc
     1801 tggtgacgca gatcttccac aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg
     1861 tgaacggcct gccggtggat cggcacggcg gttcggtggc agcgtttggt catctctatt
     1921 ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct cggcgaagtg ccgccgcaca
     1981 ccagccctgg cggctacgtg atggattcac ggccagggct ttatgattca gtgctggtgc
     2041 tggactataa aagcctgtac ccgtcgatca tccgcacctt tctgattgat cccgtcgggc
     2101 tggttgaagg catggcgcag cctgatccag agcacagtac cgaaggtttt ctcgatgcct
     2161 ggttctcacg ggagaaacat tgcctgccgg agattgtgac taacatctgg cacgggcgcg
     2221 atgaagccaa acgtcagggt aataaaccac tttcgcaggc gctgaagatc attatgaatg
     2281 ccttttatgg cgtgcttggc accaccgcct gccgcttttt cgatccgcgc ctggcatcgt
     2341 cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa agcattgatt gaagcgcagg
     2401 gctacgacgt tatctacgga gataccgact cgacttttgt ctggctaaaa ggtgcacatt
     2461 cggaagaaga ggcggcgaaa atcggtcgtg cactggtgca gcacgttaac gcctggtggg
     2521 cggaaacgct gcaaaaacaa cggctgacca gcgcattaga actggagtat gaaactcatt
     2581 tctgccgttt tcttatgcca accattcgcg gagctgatac cggcagtaaa aagcgctacg
     2641 ccgggctgat tcaggagggc gataaacagc ggatggtgtt taaagggctg gaaaccgtgc
     2701 gcaccgactg gacgccgctg gcccagcagt ttcagcagga gctatacctg cgcatcttcc
     2761 gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga caaactgatg gcgggtgaac
     2821 tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc gctgagcgag tatcagcgta
     2881 atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga agaaaaccaa aagcgtggtc
     2941 gccccttgca atatcagaat cgcggcacca ttaagtacgt atggaccacc aacggcccgg
     3001 agccgctgga ctaccaacgt tcaccactgg attacgaaca ctatctgacc cgccagctac
     3061 aacccgtggc ggagggaata ctccctttta ttgaggataa ttttgctaca cttatgaccg
     3121 ggcaacttgg gctattttga gcaaaaaaag agttcgccag ataccatttt gatgcgtgac
     3181 gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga ataacaccac
     3241 taccccataa gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca
     3301 catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt
     3361 ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa
     3421 aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat
     3481 actattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc
     3541 ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa
     3601 gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag
     3661 attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag
     3721 ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc
     3781 aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg
     3841 ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct
     3901 gctgaacgtg tgctaattat cgtcccggaa accttacagc atcagtggct ggtagaaatg
     3961 ctgcgccgtt tcaacctgcg ctttgcgcta tttgatgatg agcgttatgc cgaagctcag
     4021 cacgatgctt acaacccgtt cgacaccgaa cagctggtga tttgctcgct ggattttgcc
     4081 cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg aatgggacct gctggtggtc
     4141 gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt
     4201 gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg
     4261 gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt
     4321 gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg
     4381 ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat gatcggcgag
     4441 caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc
     4501 cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt
     4561 aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg
     4621 ctgccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt
     4681 gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt
     4741 gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc
     4801 agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg
     4861 gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg
     4921 attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta
     4981 ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc agttcgccag ccacatggtg
     5041 atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg tctggatcgt
     5101 atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac cgctcagtcg
     5161 gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg cccgaccgga
     5221 cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc accggttcaa
     5281 accgaaggct ttgacgatct gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca
     5341 cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc
     5401 caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc
     5461 atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg
     5521 ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc
     5581 accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt tattacctgg
     5641 gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac tggtagcagc
     5701 acgatttcac tattgaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt
     5761 tatgtcgttg aagcccaggc tccgaagcag ttgcagctca accgcttcct gccaccgacg
     5821 ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt agagtttgaa
     5881 acctttaacc gtcagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc
     5941 gtgcagcagg atgttcacgc tatccttcag ctgggtgaag cgcagatcga gaaatctgcc
     6001 cgtgcattga ttgatgccgc acgtaacgaa gccgacgaaa aactgtctgc cgagctgtct
     6061 cgtctggaag cgctgcgcgc agtgaacccg aacattcgtg acgacgaact gaccgccatt
     6121 gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggctggcg tctggatgcc
     6181 ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca
     6241 atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccacatt atggtggtta
     6301 acaagccgag cggtttgttg tctgtgccgg ggcgtctgga agagcacaaa gacagcgtga
     6361 tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta
     6421 ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt
     6481 tccgcgagcg cgagccgaaa aagcagtatg tagcccgcgt ctgggggcat ccatcccctg
     6541 cggaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag
     6601 tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgcggcgg
     6661 ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcag ctgcgtgtgc
     6721 atatgctggc gctgggtcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga
     6781 gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt
     6841 atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt
     6901 cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg
     6961 acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct
     7021 ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct
     7081 caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt
     7141 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
     7201 cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat
     7261 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc
     7321 gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
     7381 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
     7441 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt
     7501 ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
     7561 ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta
     7621 aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
     7681 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta
     7741 acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc
     7801 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
     7861 taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc
     7921 attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt
     7981 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
     8041 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
     8101 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
     8161 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
     8221 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
     8281 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
     8341 aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat
     8401 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
     8461 gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg
     8521 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
     8581 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
     8641 gaagaacaag ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
     8701 tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
     8761 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
     8821 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
     8881 tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat
     8941 ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg
     9001 ttgttctact ggcaacactc cggggtcatg gatcaagtgt ggcgtttcaa cgtcgactac
     9061 accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact
     9121 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
     9181 gtttcaacca agcagttcca ggtctttagc gagcagaaca ccagtagcta ctcggcagag
     9241 ccgcagttag acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
     9301 ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac
     9361 ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg
     9421 aagttgctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
     9481 accaagctgg acgaatccgt taaccgcgtt atgccgcaat tcaaagttga cggcaaaatg
     9541 gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc
     9601 gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct
     9661 ctactgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt
     9721 attgcctccg ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
     9781 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
     9841 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg ggcaggcgat
     9901 acttactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt
     9961 ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg
    10021 gtacagctga attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac
    10081 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagctgg
    10141 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
    10201 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt
    10261 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
    10321 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
    10381 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
    10441 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
    10501 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
    10561 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
    10621 aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc tgcgccacca
    10681 aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt
    10741 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
    10801 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg
    10861 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
    10921 catcaccatc ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga
    10981 cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacttc
    11041 tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg
    11101 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
    11161 ccagatgggc tggggccgta ttcaggagtt gccgggtatc ttcgcccagg cattaagcac
    11221 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
    11281 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
    11341 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgtgtga aactggaaca
    11401 gattgctgcc gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
    11461 tcaggatcca ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
    11521 cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
    11581 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
    11641 cgacgctgcg cagaaggatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
    11701 agcagcaagc tggatgcagg aacaacgcgc cagcgcctac gttaaaatcc tgagcaacta
    11761 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
    11821 ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc
    11881 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
    11941 cccaactccc ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta
    12001 cgcgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt ggtggagacg
    12061 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
    12121 gtgcataaag gcgtcattaa cgacgcaggt attccgttta ctggtcatac cgagtttttc
    12181 gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
    12241 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt
    12301 ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa
    12361 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
    12421 gaagagatag acaccattat tccggtgctc aacgagctgc gggcgcaggg gatgaaactc
    12481 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
    12541 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
    12601 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
    12661 cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc
    12721 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
    12781 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
    12841 ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga
    12901 ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
    12961 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
    13021 cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg
    13081 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
    13141 ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac
    13201 cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
    13261 cagtgctgga agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
    13321 tgcgcctggt tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc
    13381 gcatcaccac tgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
    13441 tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata
    13501 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
    13561 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
    13621 ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
    13681 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
    13741 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
    13801 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
    13861 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
    13921 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
    13981 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa
    14041 agataccctc tggctgacgg gcgatctggt cgcgcgcggg ccgggttcgc tggatgttct
    14101 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca
    14161 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
    14221 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca
    14281 aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg atcacgccgc agtgggatct
    14341 acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctatcgagtg actcctatcc
    14401 cttctttctc gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
    14461 gctgggaaga ctgcgtttta tcaccaacgc ttttacccgt atgcgttttt gcttcccgaa
    14521 cggtcaactg gatatgtaca gcaaagaatc gccggaagag gcccctgccc cactgaaacc
    14581 gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc
    14641 atcgctggag ggcaaaggta cgccggaagg tatatacgcg ctggataccg gctgctgctg
    14701 gggtggtaca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
    14761 ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata taggaaggcc
    14821 ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac aaaattaccg
    14881 ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcagcat cgtggaattc
    14941 gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac
    15001 ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt ggcaggaact gctcataaac
    15061 gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcatc
    15121 caccgacttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt
    15181 tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca taatcacggg
    15241 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc
    15301 gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat
    15361 ttcccgataa aaaaaattgt cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc
    15421 gaggatgatg ttgaggaaaa ttttatattc tgctggcgag tccacgctct ctccctggac
    15481 tcgccgcatt acaatgaaac aaaaacaaac agttagctgt aaagtgtgat ttacgtcact
    15541 ctttattagg atgagggttt cgtttccggt tcatccgcca tgttgccggt atgtttacct
    15601 tcttcggttc cctgccagcc atgtcgttga attaatgaca gatgttcgcg gtcctcggta
    15661 atgatctcac ttaacatcgc gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtcg
    15721 ttctcaacca ttgccatctc ttccaccatc tgaatattaa agcggcggaa cacatcggca
    15781 cgttctcgcg cttcatacgg ccccagacct aaactttcca gtgccagacg cccggttttc
    15841 agcgcacctt cgaaggtttc acgctccggc ttttcaacgc ctgcctgacg caaacgaatg
    15901 tagtggtcga catcgcgggc gcgggcaata atctgcaaat gcgggaagtg ttctttcacc
    15961 atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg cgttaatcag cacttccgct
    16021 ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg catcgccata aaacactttc
    16081 ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt gatcgagcac caccattttc
    16141 accccgctgg agagcagtaa acgtccggtg atctgcccaa aacgaccgaa tccggcaatg
    16201 attacccgcg gctgttcttc gtcgatctca tcagcttcac gcgcctcttc agtagaagat
    16261 tgctcaaggc gattgaggat caccagcaga atcggcgttg ccgccatcga cagcgccacc
    16321 gccagggtca gcgatttcgc ccactccggt tccagcacat tcgccatctg cgccgtgcca
    16381 aataccacaa aggcaaactc actgccctgc cctaacaaca ccgcaaacca acgacgctgt
    16441 ttatttggca cttgcaacgg tcgggcaatc agccacagca tggcgatttt gatgatgagg
    16501 aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa gcagcgtgcc aaagtctatc
    16561 gacatgccaa caccgatgaa aaacagcccc aacagcaaac ctttaaatgg ttcgatatcg
    16621 ctctccagcg catgacggta ttccgagctt gccagcagta cgcccgccag aaacgcgccc
    16681 atcgccatcg acaagccgac ctcttccagc agcaaaccaa agccaaacac gaggaataac
    16741 gccacggcac taaacacttc ccgcaagcca gagcgggcta caaaacgcag cgccggacgc
    16801 gtgacatagc gccccagcaa taccaccagc accagcgcac ccgccacttt taacgccgag
    16861 agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg ccagtagcgg aatcatcgcc
    16921 accagcggga tcgccgcgat atcctggaac agcagcaccg caaaggcact gcgacccatt
    16981 tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg caatcgccgt agaggagagc
    17041 gccagcgtca tgccgatcaa ttccgcgacc tgccagtgca gcccaagtaa catgcagaac
    17101 agccccagca ggccgccgca aatcaccatc tgcaatgcgc caccgccgaa cactgccgca
    17161 cgcagcttcc acagcctttg tggatcgagt tcgaggccga taataaacag catcagcacc
    17221 accccaatct cggcaaagtg cagaatagat tcggcatcgg tcaccagtcg cagcccccac
    17281 gggccaataa tgcagccggc gatcaggtag ccaagtaccg atcccagacc aagacgtacc
    17341 gcaatgggta caatcagcgc tgccgaaccg agataaatca gcgcctgaat cagcgtatgg
    17401 ctatccatga tgggcctcct gccattccag cagacgttgc ttatagtgac gcgcctgccc
    17461 ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt
    17521 cagcccgcag tagattgccg tcgcctgtag cggctgcgac agcacatcaa agcccggatg
    17581 cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc accgcccaca gcaaatattt
    17641 gccatgcagc gccttgccgc cgtgaccgta agcccagccg tgcgacaaaa ctttatcgat
    17701 ccaaagttta agaagcggag gaatgctgta ccactgcatc ggatgctgcc agacgatcag
    17761 atcggcgcga gacagcgcct cctgctcggc ggcaatatcg atattgaagt caggatagag
    17821 ttgataaaga gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt
    17881 attcgcatgg gaatgatgcg gatacggatg cgcataaatt ataagaatca taggtagccc
    17941 gcgtgattac gcctttgttt tcccacaaag tgtagtcata tattcacgcg gcttatagtc
    18001 aataatattg attcagaaaa gagagaaatc ttaacaattt accccattct ctgcccgcgg
    18061 atcgtcatat tgctggtttg cgccagtgac atccctcgag tctccggggc aaacgctacg
    18121 gaaatcaaca agccaaacag cgagataccc gcctccatta gcatcgtgtt actgataccg
    18181 taattattga taaagatcgg cagtgcccag gtcgaaacaa tggtgccaat acggcttaag
    18241 gacataatca cgcccacggc agaggcgcgg atatctgtcg ggaagagttc attaggatag
    18301 agccactgca aattacccgg cccgccagag aaaaaggcat acaccgcaaa ggccatcact
    18361 accagccaga tccccatatc cgggatcagc cccaaaaccg ccagcgccag cgtcatcatg
    18421 gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt ttaaccacag catcggcgga
    18481 atacagccga gcataaagaa caggctaatc accacgttcc ccagcgccgc gtttttgcca
    18541 acacccaatc ccaacaaacc aacgatttgc gggccaaagg tgtaaatggc gaacattggg
    18601 atcacctggc aggtccagat ggcggcaaca aacagaacaa aaggaaaatg gcggcgatta
    18661 aacagatcgc gaaaacgggt ttgctacggc tgctcttcat cgaaagccac cggttcgcca
    18721 aacagtttta tcatcatctc ttcgcactct tttactcgcc ctttgcgtaa taaccagcgg
    18781 ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca aacaggggat cgccgcgcta
    18841 cccagcatcc agcgccagcc gccttccaca tcataaagcc agtagccgac cagatcggcg
    18901 caggtcgcgc cgacatacca catggcggcg ataaagctga tggaaaatgc ccgctgacgg
    18961 gtactggaga actcggtgat cattgaggtg gcgatgggat aatctgcacc gatgacaatg
    19021 ccgataagta cccgcatcac caacagttcg acgggggatg aaacaaacat cgtcgccacc
    19081 gatatcacgc cgatggcgat gatatcaatg aggaacattt tgcgccgtcc tactttatcg
    19141 gaaatatagc caaacagcga tgtgccaacg aacagcccgg cgagcgttcc cgcgcccaac
    19201 aagccaatcc agtcagcgtc cagttttagc gccggagtca gttgctccag cgccacgcca
    19261 atcattacca gtacataacc atccagaaac ggaccaccgc ttccccacag caaaatgcgg
    19321 cggtgaattg aggagaattt gagatcgtca aagtttctgg acggttgcat gggttattcc
    19381 tgtttttttg caatgagagt cagtacggcg ttacgtatca aaccaacatc agccgtaacg
    19441 gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg accccagccc
    19501 cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca cactgccgtc
    19561 atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca gggcctgttt
    19621 atcggcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga atttattgac
    19681 gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc tttcatgcca
    19741 tccttgatca gattgatgaa gcccactttc ttgccgtggc ggaggatttt cttgcgcatc
    19801 agttccggcg cactgccgtc aatggtgaac aggtcacgcg ccacgcccac cgccagttcc
    19861 gggtagccgc taaacatgcg tgggttatca aggaaggccg gtagtttctg gtacatacgc
    19921 atatcgcgca gcgggccact ctcaagatgc tgacgatatt ccgccagttt ttgcttactg
    19981 aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg cggcttcccc ggcggcaatc
    20041 gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc ggcatcaccg
    20101 gcaatcaata cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc ttccggcact
    20161 acgtgagcgg aatattccac cagcttgccg cccgcgatca gcggtgccac tgccggatgc
    20221 tgtttgaaat cttccagcat ttgcggcacc gatttttttg cgtcatgcag atgatgcaga
    20281 ccacaaacca gccccagcga cagggtattt tcattggtat aaaggaagcc gccgcccatc
    20341 aggccatcgg tgggtgatcc cgcaaacaga caagccgcgc cctgattacc ctgcaactga
    20401 aaacggtctt cgattaccga cttcggtaac tcgatcagtt ccttcacgcc aaccgccaca
    20461 tccgtcggtt taacgcgttt tgccatcccc agcttttcgg caaggatgga gttcactcca
    20521 tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cggcttctac accgacgact
    20581 ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg cgcgcccgct
    20641 tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga gtaagaacgc
    20701 tgggatggcg aggtttcgtc accgttgcag tagttcatag tcatcgctga cttttccgtc
    20761 ataaacgcga gtttttcatg ggtgatcagg cgttctaccg gggcggattc ggcgaaacca
    20821 ggaataatgt gttccaggct gtgggcatag agacgcccgc cggtgacgtt cttggcacct
    20881 gcggaattgc cacgctcgat aactaacact tgcgcccctt cgcgggcgag caccagtgcg
    20941 gcaaccgaac cggcaagccc agcaccgacg atgatggcgt caaagatatc ttcggacata
    21001 agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat caacgcgcta
    21061 aagctgcggt cagcgccgga aggatcttca cggcgtcacc aacaatgccg taatccgcgt
    21121 actggaagat cggcgcattt ttatctttat tgatggcgaa aatggtttgc gatgcattag
    21181 caccaaccat gtgctggatc tgcccggaga tccccaccgc caggtacagt tcaggtttca
    21241 gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg ttttccgcca
    21301 ccggacgaga acaggccaac tccgcaccta tcgccttgca aagctgttct gccagcgcaa
    21361 tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagacgggct ttgtcgagat
    21421 cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca gccggagcct
    21481 gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc gcatcgaacg
    21541 tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca
    21601 gaccaccgta aaccatatgt ttcactgttg ctttaccgtc ctgtacgctg acggtgctgg
    21661 catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt aatttgccgc
    21721 gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg ctggcgaata gtgtcagcca
    21781 tgacaccggc gtaatcttcg atcatccgat cgtccggttt gccgtttaat ttccagacat
    21841 gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca aaggtgttga
    21901 tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa ggggtatcgc
    21961 tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa tgactttgcg
    22021 aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg
    22081 acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct ctgcgttaaa
    22141 accaatatcc gccgccgacc atacctggac gggctttttc gccgcgccga gaatggcttt
    22201 catcgaagga atttgtgggg aattgatatc agtggaaaca gcaacaaccg caggcagcgg
    22261 aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg tatctgccgt
    22321 cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt cgcccaccag
    22381 cagaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa
    22441 gcctgctttc tgggcggctg cagccagtgc gctcgccgtt tgttgcggca gtgcctgctc
    22501 gaactggtca tcaatcacca caatcagttc atccgggccg cgcgatagta catctttacg
    22561 ccctttggcg ttggtcaggg ctttaccgcc cacacttaag gccgtcacct gcgcctctgc
    22621 tgcctgttgc tttagctggc aagccgcttc aatagcgttc agatcgtatt ggcttatttt
    22681 ggcatcggct ttgctgaagt ctaatgaacc atcagcatta ttgaccgcaa tatcctgttc
    22741 atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa tcatgaagga
    22801 aacgtcgttc atatcggtgg tgttaattac agatatcacg ccctttaatt tggaaataaa
    22861 actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc aatattgtta
    22921 agttcctcac caatattgaa aacatggcgt agaaaaaaga aattttcaat attgctttat
    22981 ggatcaccaa tattgaaagt cagatgctat ttttttactc acaacacaac ataacaaact
    23041 gattaataat caaaattaac gagaaaagcg ccctctattc acccgaaaaa aaatgtgata
    23101 acaatcacag aatgcagctt attgaatacc cattatgagt tagccattaa cgcgtccacg
    23161 aggttaataa taattatatc aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa
    23221 atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga
    23281 accgaaggtt ttctttccgc cgttaataat cgtcggcata ctttgttggc ttacagtcag
    23341 agatctggat gcagcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg
    23401 gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct ggctggtgtt
    23461 tggcccgtat gccaaaaagc gtttaggtaa cgaaccacct gaatttagta ccgccagttg
    23521 gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg gatcgattga
    23581 gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga caggggcgaa
    23641 agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg ccacttacag
    23701 cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag tgattcgccc
    23761 cagctcgaca ctggtgccgc tggtaggtga aaaacacgcc aaagggttgt tcggcactat
    23821 cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc
    23881 cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata ccctgcaact
    23941 ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg cttgcggtct
    24001 gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg
    24061 ttgggtgttc attgtcagcg gtgccagctt catcatgaac tacttcaccg attcggtggg
    24121 gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg
    24181 cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttatg ctatccagat
    24241 gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat
    24301 ggtgctgggg ctgacagcgt caacctggat cctgtggact gtactcggta gtaacactct
    24361 gctgttgata gataaaaaca tcatcaacat tccaaatctg atcgaacagt acggtgtggc
    24421 gcgcgccatc attgaaacct gggccgctct gccactcagc accgccacca tgtggggctt
    24481 cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt ataccctggc
    24541 gatgtccact tgccgcgaag tacgcgatgg tgaagaacca cctctgctgg tgcgtatcgg
    24601 ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc
    24661 gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac
    24721 gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc ccaaaatatc
    24781 aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg tcgccggtat
    24841 ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg accgtgacag
    24901 cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca gtctgctgat
    24961 ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg tgtggatgga
    25021 gctgggacgt ctgggggcac caacctatgt gctgtaccag ttgccgggcg ggttcaacac
    25081 cttcctgcgc gaaggcacac aagagcagat cgacaaaatt atggctttcc gcggcaccgg
    25141 taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg tgggtagcct
    25201 gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta agtgttttat
    25261 taccagcagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga
    25321 caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa
    25381 acttgaaaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg acgacgtgga
    25441 actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga
    25501 gttcgaccat gaacgtttcc tggtagccct caccaactac ggtacggcga tgtgcgcttt
    25561 tgaagatgcg gcgcgctacg ccaatcagcg cgtgcagttt ggcgaggcta ttggtcgttt
    25621 ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat
    25681 gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg atgcagcgat
    25741 gtgcaaatac ttctgcgcca atgcggcatt tgaagttgtg gatagcgcaa tgcaggtgct
    25801 gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg atctgcgtgt
    25861 agaccgcgtc tccgggggat ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct
    25921 gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctaatgcgtc ttatcaggcc
    25981 tacgggagca cgaatgtagg ccggataaag cgtttacgcc gcatccggca gtcatgcgct
    26041 acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt cgggccgttg
    26101 gccggattgc gcgttgtctt ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg
    26161 ttcgcagaat ggggtgcgga agttatctgg atcgagaacg tcgcctaggc cgacaccatt
    26221 cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat
    26281 attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc
    26341 atcgaagcca gcaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg
    26401 cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta cggcaccgag
    26461 gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg ttacctgatt
    26521 cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga ttacttttct
    26581 ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga aaccggtaaa
    26641 ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca gtacttcatg
    26701 atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac
    26761 tacgccggtt gcggtctgta taaatgcgcc gacggctaca tcgtgatgga actggtgggc
    26821 attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact cggtacgccg
    26881 gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg cccactggta
    26941 gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa agaacgcttt
    27001 gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga aagcaatcca
    27061 cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg cacctgcaaa
    27121 gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg cggaatgccc
    27181 tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga aaacgacatt
    27241 caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg
    27301 cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag gtgcaatgga
    27361 tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg tttacggtca
    27421 taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata gttatcttga
    27481 gttaaatcag gagattaacc gcacgacaaa cctgttttat acgctgggga ttcgcaaagg
    27541 cgacaaggtt gctctacatc tcgacaactg cccggaattt atcttttgct ggttcgggct
    27601 ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttgcgcg aggaaagcgc
    27661 gtggatcctg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat tctatcctat
    27721 gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc tgacagatgt
    27781 ggcacttccc gctgatgatg gcgtgagttc gtttactcaa ctgaaaaatc aacaacctgc
    27841 caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc tcttcacctc
    27901 cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc gcttcgctgg
    27961 atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacctga cggtaatgcc
    28021 tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg ccggggccac
    28081 ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggtac agaagtaccg
    28141 cgccaccgtt accgaatgta ttccgatgat gatccgtacc ttgatggcgc agccgccttc
    28201 agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact tgtcggagca
    28261 ggaaaaagat gcgttttgtg aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac
    28321 ggaaaccatt gtgggcatta tcggcgatcg ccctggcgat aaacgacgct ggccgtcgat
    28381 tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc gcccgctccc
    28441 ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca tcttcaaaga
    28501 gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct ggctacatac
    28561 cggcgatacc ggatactgcg acgaagaggg ctttttttat ttcgtcgatc gccgctgcaa
    28621 tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata tcatcgccac
    28681 ccatccaaaa attcaggata tcgtggttgt gggtattaaa gattcgattc gcgatgaagc
    28741 catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg aatttttccg
    28801 cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga tcagaaaaga
    28861 tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa tgaaacagca
    28921 gggaactacc ctgccaacta ataatcacgc tataaaacaa tatgcatttt ttgcagggat
    28981 gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac
    29041 ccgcaatgga tcaattctgg aaattaccct tgatcgtcca aaagcgaatg ctattgatgc
    29101 aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg
    29161 tgtcgccatt attaccggtg ccggagagaa gttcttttcc gcgggctggg atttaaaagc
    29221 ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggatttg cgggattaac
    29281 cgaaattttc aatctcgaca aaccggttat cgcagctgtg aacggctatg cctttggcgg
    29341 cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg ccagcttcgc
    29401 cctgccggaa gccaagctgg gcatcgttcc tgacagcggc ggcgtgctgc gtctaccgaa
    29461 gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga
    29521 agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac tgatggataa
    29581 cgcccgcgaa ctggcgcagc agctggttaa cagcgccccg ctggcgattg cggcgctgaa
    29641 agagatcttc cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag
    29701 cggcgtgttg aaacactatc cgtcggttct gcattcggag gatgccattg aagggccgct
    29761 ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt gagctattac
    29821 gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc
    29881 ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc ctcactgcgt
    29941 ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg
    30001 catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcacggagcg
    30061 atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag cgtgattatg
    30121 gatggcgcgg tcattggcga agagagcatt gttgccgcca tgagctttgt caaagcaggc
    30181 tttcacggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt
    30241 agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga tcttgtcggg
    30301 cgctgtcatg catcgttaca tgaaacacag ccactgaggc aaatggagga aaatcgcccc
    30361 cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat gcggcgtaaa
    30421 cgccttatcc aactctgctg gagagcaacc gtaggccgga taagatgcgc cagcatcgca
    30481 tccggcgata gctgtcggat gcggcataaa cgccttatcc aacccggctg gagagcaacc
    30541 gtaggctgga taagatgcgc cagcattgca tccagcaacc aggctgcgtg gtatcaacga
    30601 aatgacatta acgacgcata ctctttgaca acatcatctg ccacttctgc tcgcggttaa
    30661 gttccgtgag cggaacggca ggaatacgcg gcgtctttct tacgccgact aatttatccc
    30721 ggctgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact tttaccagtc
    30781 gctggcactg gcatccccgt ccttccagct tattagggat catcttaact gcgcagctta
    30841 tttctgcgac ttccgacaga atataagtca gggtattaac cgccttgctg tgttcaatat
    30901 cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc atccattcgg
    30961 cgattaacaa gtagggtggt ttttcaacat atccttcaca catacgaact ccagtttcac
    31021 aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat attaaaattg
    31081 tggatatatc gatagtatgc aacttcaccg tcgataa
//
LOCUS       DAZPBM010000030        30986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPBM010000030 DAZPBM010000000
VERSION     DAZPBM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.030"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHUZQ_001292"
     CDS             59..1423
                     /locus_tag="ACHUZQ_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1025029.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUZQ_001293"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUZQ_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1025030.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHUZQ_001294"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHUZQ_001294"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1025031.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUZQ_001295"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUZQ_001295"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1025032.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUZQ_001296"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUZQ_001296"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1025033.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUZQ_001297"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUZQ_001297"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1025034.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUZQ_001298"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUZQ_001298"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1025035.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUZQ_001299"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUZQ_001299"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1025036.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUZQ_001300"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUZQ_001300"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1025037.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUZQ_001301"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUZQ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1025038.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUZQ_001302"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUZQ_001302"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1025039.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUZQ_001303"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUZQ_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1025040.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUZQ_001304"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUZQ_001304"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1025041.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUZQ_001305"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUZQ_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1025042.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUZQ_001306"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUZQ_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1025043.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHUZQ_001307"
     CDS             13749..14288
                     /locus_tag="ACHUZQ_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1025044.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUZQ_001308"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUZQ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1025045.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUZQ_001309"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUZQ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1025046.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUZQ_001310"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUZQ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1025047.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUZQ_001311"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUZQ_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1025048.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUZQ_001312"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUZQ_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1025049.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUZQ_001313"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUZQ_001313"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1025050.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUZQ_001314"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUZQ_001314"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1025051.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUZQ_001315"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUZQ_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR1025052.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUZQ_001316"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUZQ_001316"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1025053.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUZQ_001317"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUZQ_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1025054.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUZQ_001318"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUZQ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1025055.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUZQ_001319"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUZQ_001319"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1025056.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUZQ_001320"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUZQ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1025057.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUZQ_001321"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUZQ_001321"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1025058.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..>30986
                     /locus_tag="ACHUZQ_001322"
     CDS             30847..>30986
                     /locus_tag="ACHUZQ_001322"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1025059.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLL"
BASE COUNT         7623 a         8408 c         7828 g         7127 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgct
//
LOCUS       DAZPBM010000031        30921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPBM010000031 DAZPBM010000000
VERSION     DAZPBM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.031"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            519..1034
                     /locus_tag="ACHUZQ_001323"
     CDS             519..1034
                     /locus_tag="ACHUZQ_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1025060.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1252..1980
                     /gene="artP"
                     /locus_tag="ACHUZQ_001324"
     CDS             1252..1980
                     /gene="artP"
                     /locus_tag="ACHUZQ_001324"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1025061.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1998..2729
                     /gene="artI"
                     /locus_tag="ACHUZQ_001325"
     CDS             1998..2729
                     /gene="artI"
                     /locus_tag="ACHUZQ_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1025062.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2736..3452
                     /gene="artQ"
                     /locus_tag="ACHUZQ_001326"
     CDS             2736..3452
                     /gene="artQ"
                     /locus_tag="ACHUZQ_001326"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1025063.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3452..4120
                     /gene="artM"
                     /locus_tag="ACHUZQ_001327"
     CDS             3452..4120
                     /gene="artM"
                     /locus_tag="ACHUZQ_001327"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1025064.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4412..5143
                     /gene="artJ"
                     /locus_tag="ACHUZQ_001328"
     CDS             4412..5143
                     /gene="artJ"
                     /locus_tag="ACHUZQ_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1025065.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5318..6445)
                     /gene="rlmC"
                     /locus_tag="ACHUZQ_001329"
     CDS             complement(5318..6445)
                     /gene="rlmC"
                     /locus_tag="ACHUZQ_001329"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1025066.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6486..6974)
                     /locus_tag="ACHUZQ_001330"
     CDS             complement(6486..6974)
                     /locus_tag="ACHUZQ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1025067.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(7034..7879)
                     /gene="potI"
                     /locus_tag="ACHUZQ_001331"
     CDS             complement(7034..7879)
                     /gene="potI"
                     /locus_tag="ACHUZQ_001331"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1025068.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7876..8829)
                     /gene="potH"
                     /locus_tag="ACHUZQ_001332"
     CDS             complement(7876..8829)
                     /gene="potH"
                     /locus_tag="ACHUZQ_001332"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1025069.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8839..9972)
                     /gene="potG"
                     /locus_tag="ACHUZQ_001333"
     CDS             complement(8839..9972)
                     /gene="potG"
                     /locus_tag="ACHUZQ_001333"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1025070.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10067..11179)
                     /gene="potF"
                     /locus_tag="ACHUZQ_001334"
     CDS             complement(10067..11179)
                     /gene="potF"
                     /locus_tag="ACHUZQ_001334"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1025071.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11530..12006)
                     /locus_tag="ACHUZQ_001335"
     CDS             complement(11530..12006)
                     /locus_tag="ACHUZQ_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1025072.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12094..12996)
                     /gene="rimK"
                     /locus_tag="ACHUZQ_001336"
     CDS             complement(12094..12996)
                     /gene="rimK"
                     /locus_tag="ACHUZQ_001336"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1025073.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13057..13779)
                     /gene="nfsA"
                     /locus_tag="ACHUZQ_001337"
     CDS             complement(13057..13779)
                     /gene="nfsA"
                     /locus_tag="ACHUZQ_001337"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1025074.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13763..14050)
                     /locus_tag="ACHUZQ_001338"
     CDS             complement(13763..14050)
                     /locus_tag="ACHUZQ_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1025075.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14210..14467
                     /gene="grxA"
                     /locus_tag="ACHUZQ_001339"
     CDS             14210..14467
                     /gene="grxA"
                     /locus_tag="ACHUZQ_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1025076.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14497..14874)
                     /locus_tag="ACHUZQ_001340"
     CDS             complement(14497..14874)
                     /locus_tag="ACHUZQ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1025077.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            15144..16829
                     /locus_tag="ACHUZQ_001341"
     CDS             15144..16829
                     /locus_tag="ACHUZQ_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1025078.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(17004..17540)
                     /gene="rcdA"
                     /locus_tag="ACHUZQ_001342"
     CDS             complement(17004..17540)
                     /gene="rcdA"
                     /locus_tag="ACHUZQ_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1025079.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17624..18832
                     /locus_tag="ACHUZQ_001343"
     CDS             17624..18832
                     /locus_tag="ACHUZQ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1025080.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            18832..19647
                     /gene="ybjI"
                     /locus_tag="ACHUZQ_001344"
     CDS             18832..19647
                     /gene="ybjI"
                     /locus_tag="ACHUZQ_001344"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1025081.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19733..20017
                     /gene="ybjH"
                     /locus_tag="ACHUZQ_001345"
     CDS             19733..20017
                     /gene="ybjH"
                     /locus_tag="ACHUZQ_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1025082.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(20058..21285)
                     /gene="mdfA"
                     /locus_tag="ACHUZQ_001346"
                     /pseudo
     CDS             complement(20058..21285)
                     /gene="mdfA"
                     /locus_tag="ACHUZQ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            21570..22166
                     /gene="ybjG"
                     /locus_tag="ACHUZQ_001347"
     CDS             21570..22166
                     /gene="ybjG"
                     /locus_tag="ACHUZQ_001347"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1025083.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            22224..22982
                     /gene="deoR"
                     /locus_tag="ACHUZQ_001348"
     CDS             22224..22982
                     /gene="deoR"
                     /locus_tag="ACHUZQ_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1025084.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(23029..24231)
                     /gene="dacC"
                     /locus_tag="ACHUZQ_001349"
     CDS             complement(23029..24231)
                     /gene="dacC"
                     /locus_tag="ACHUZQ_001349"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1025085.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24478..25103
                     /gene="gstB"
                     /locus_tag="ACHUZQ_001350"
                     /pseudo
     CDS             24478..25103
                     /gene="gstB"
                     /locus_tag="ACHUZQ_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(25100..26215)
                     /gene="yliI"
                     /locus_tag="ACHUZQ_001351"
     CDS             complement(25100..26215)
                     /gene="yliI"
                     /locus_tag="ACHUZQ_001351"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR1025086.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26326..26709)
                     /gene="bssR"
                     /locus_tag="ACHUZQ_001352"
     CDS             complement(26326..26709)
                     /gene="bssR"
                     /locus_tag="ACHUZQ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1025087.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26922..28247
                     /gene="rimO"
                     /locus_tag="ACHUZQ_001353"
     CDS             26922..28247
                     /gene="rimO"
                     /locus_tag="ACHUZQ_001353"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1025088.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28294..29615)
                     /locus_tag="ACHUZQ_001354"
                     /pseudo
     CDS             complement(28294..29615)
                     /locus_tag="ACHUZQ_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(29623..>30921)
                     /locus_tag="ACHUZQ_001355"
     CDS             complement(29623..>30921)
                     /locus_tag="ACHUZQ_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR1025089.1"
                     /translation="NSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYL
                     AVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCREN
                     VQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMF
                     SEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWV
                     SAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSP
                     WQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISI
                     DDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGV
                     ESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7799 a         7971 c         7483 g         7668 t
ORIGIN      
        1 attcattagc ttaaattctt tacccacaat tgtagcataa ataatattat cgagagtcaa
       61 agctgggact atcaaatttc gctcattttc aaaaatacaa cgtaattttg gcaatcatct
      121 taatcgcagg atctcttatc gcatgtgaaa aattttgtaa tgattagaca ataaccgcgt
      181 tcatttatca cgcgttataa atgtgaaact ggcttcttgc agatgctctt aacctctaca
      241 ggaggattta taccaccatt tcagaactat catatttacc ttaaatgatc atttcagcca
      301 tataacacgc tcaacctcac aaatggttta cactgtctat tgtttgggta atttaagtaa
      361 gtcagctacg agcaaccttg agtggatagc cagcaatgga ctcaaaccca atcaggtgtt
      421 cagattgttt cagaattgta ttaaaaacaa tgtattaaat aatctgatag cgtttggtcg
      481 tagtgtgacg cagacagcaa gctaataagg aaatgattat gcgctaccgt tcactatctc
      541 tacttattcc atgtgcactg ctgctgagtg cctgtaccac cgtcacgcca gcttataaag
      601 ataacggtcc ccgcaccggt tcctgtgtcc agggggggcc tgacagcgtg gctcaacaat
      661 tctatgacta ccgcattcaa catcgcagca atgacattac cgccctgcgc ccctatttga
      721 gtgacaaact ggcaaccctt cttagcgatg ccagccgcga taatagtcat cgtgaattgt
      781 taagcagcga tccgttctcc agtcgaacca ccttgccaga tagcgcccac gttgccagtg
      841 catcgactat ccctaatcgt gatgcccgta acattccatt gcgcgtagat ctcaaacagg
      901 gtgaccaggg ttggcaggat gaagtgttaa tgattcagga aggccagtgt tgggtcatcg
      961 acgatgtgcg ctacttaggc ggcagcgttc atgccacggc gggtacgctc cgtcagtcca
     1021 tcgagaatcg ctaacgcctt gatcaaccaa taaaccccgt aaattgtccg gcatttcgtg
     1081 aggaatgccg acatttatgc tcgccgacca ccgcccccgt tattttgtgc tatgtttatt
     1141 gaataatgcg ctttgctttt aacttttaaa gcagaaatat tgcataatta ttctgtcaaa
     1201 ggtactatct tcgacctcaa tagctatcag actgccagta tacgagtgtc aatgagtatt
     1261 caattaaacg gcattaattg cttctacggc gcgcatcagg cgctgttcga tatcacgctg
     1321 gattgcccac agggcgaaac gctggtgtta cttggcccca gcggcgcggg taaaagctcg
     1381 ctgctgcgtg tactcaatct gcttgagatg ccacgctccg gtacgctcaa cattgcaggc
     1441 aaccatttcg atttcaccaa aacaccctct gacaaagcga ttcgcgattt gcgtcgtaac
     1501 gttggcatgg tgtttcagca atacaacctg tggccgcatc tgaccgtgca gcaaaacctg
     1561 attgaagcgc cctgccgtgt actggggttg agtaaagatc aggcgctggc ccgtgcagaa
     1621 aaactgctgg aacgtctgcg tctcaaacct tatagcgatc gttacccgct gcatctttct
     1681 ggtggtcagc agcagcgtgt tgctattgcc cgtgcgttga tgatggaacc gcaggtactg
     1741 ctgttcgatg aaccgaccgc cgcactggac ccggaaatta cggcacaaat cgtcagcatc
     1801 attcgtgagc tggcagaaac gaatattacc caggtgatcg tcacccacga agttgaagtg
     1861 gcgcgtaaaa ccgccagccg agtggtgtat atggaaaatg gtcatatcgt agaacaaggc
     1921 gacgcgagct gctttaccga gccgcaaacc gaagcattta aaaactatct ctctcactaa
     1981 gattcgggat aacaacaatg aaaaaagttc tgattgccgc gttaattgca ggttttagtc
     2041 tttccgccac agctgccgaa accattcgtt ttgctaccga agcatcctat cctccgtttg
     2101 aatcgattga tgcaaacaac cagatcgttg gttttgacgt cgacctggca caagcgctgt
     2161 gtaaagagat tgatgcaacc tgcactttct ctaaccaggc gtttgacagc ctgatcccaa
     2221 gcctgaaatt ccgtcgcgta gaagccgtga tggcgggcat ggatatcact ccggagcgtg
     2281 aaaagcaggt gctgtttacc accccgtact atgacaactc tgccctgttt gtgggtcagc
     2341 aaggcaaata caccagtgtt gatcagctga aaggcaaaaa agtcggcgta cagaacggga
     2401 cgacacacca gaaattcatt atggataagc acccggaaat cactaccgtt ccgtatgaca
     2461 gctaccagaa cgcaaaactg gatctgcaaa acgggcgtat cgacggcgtc ttcggtgaca
     2521 ccgcagtggt cactgagtgg ctgaaagata acccgaaact ggcggcggtg ggcgacaaag
     2581 tgaccgataa agattacttc ggcactggcc tcggcatcgc ggtacgtcag ggcaacactg
     2641 agctgcagca gaaactcaac actgcgctgg aaaaagtgaa gaaagatggc acttacgaaa
     2701 ccatctacaa caaatggttc cagaagtaat tcctgatgaa tgaatttttt cctttagcaa
     2761 gcgccgccgg gatgaccgtc ggccttgccg tttgtgcatt gattgtcggg ctggcgctgg
     2821 cgatgttctt tgcggtatgg gagtcggcaa aatggcgtcc tgtcgcgtgg gcaggttcag
     2881 cgctggtaac cattctgcgt ggcctgccag aaattctggt ggtgctgttt atctattttg
     2941 gctcctcgca gctgctgctg acgctttcgg atggcttcac tatcaatctt gggttcgtgc
     3001 agatcccagt gcagatggac attgagaact tcgacgttag cccgttcctt tgtggtgtca
     3061 tcgctctgtc actgctgtat gccgcctatg cctcgcaaac gcttcggggc gcgttgaaag
     3121 cggtgccggt gggtcagtgg gagtctggtc aggcgctggg gctgtcgaaa tcggctatct
     3181 ttttccgtct ggtgatgccg cagatgtggc gtcatgcgct gcctggcctc ggtaaccagt
     3241 ggctggtgct gctgaaagat accgcgctgg tcagtttgat tagtgtgaat gatttaatgc
     3301 tgcaaacaaa aagcatcgct actcgtaccc aggaaccatt tacctggtac attgtggcgg
     3361 cggcgattta cctggtgatc accctgctca gtcagtacat tctcaaacgc attgacctgc
     3421 gcgcgacacg ttttgagcgg aggcccagct aatgtttgag tatttacccg aactgatgaa
     3481 agggctacac accagcctga cgctaaccgt tgcctcgctg attgtggcac tgattctggc
     3541 attgattttt accatcatcc tgacgctgaa aacgccggtg ctggtgtggc tggtgcgggg
     3601 ttatatcacg ctgtttaccg gtacgccgct gctggtgcag atcttcctga tttattacgg
     3661 gccgggccag tttccgacgt tgcaggagta tccggcactg tggcatttgt tgtcagaacc
     3721 gtggttatgt gcgctgattg cgttatcgct gaacagtgcg gcgtatacca cgcagctgtt
     3781 ttacggtgca attcgtgcga tcccagaagg tcagtggcag tcctgtagcg ccctgggaat
     3841 gagcaaaaaa gatacgctgg cgctcctgct gccgtatgcc tttaaacgct cgctctcttc
     3901 ttattccaac gaagtggtgc tggtgttcaa aagtacctct ctggcttaca ccattacgct
     3961 gatggaagtg atgggataca gccagttgtt gtacggacgc acctacgatg taatggtgtt
     4021 cggtgcggca gggattattt acctggtcgt caacggcctg ctgacgctga tgatgcgtct
     4081 gatcgagcgc aaagcactgg cattcgaacg gcggaattaa tgtagctgcg tacattcgcc
     4141 gggggggttt gtaggtctga taagacgcgc cagcgtcgca tcagacatca gcacggtgcc
     4201 tgtgccggat gcggcgtgga cgccttatcc ggcctacata gtgcataaat tcttataatg
     4261 aagacggaca actcactaag ttatccgtct ttttttattt catttaaatt atttaatcat
     4321 atttattgca tataaattca cttgatggca ttgttatccc atgccgcaga cacggccaaa
     4381 aatcataaga ttgacagacg ggagttccat catgaaaaag ttagttcttg ccgctttact
     4441 tgcttccttt actttcggtg cttctgccgc agagaaaatc aattttggcg tttcagccac
     4501 ctatccaccc tttgaatcta taggtgctaa taatgagatt gtcggctttg atatcgatct
     4561 ggcaaaagcc ttgtgcaaac aaatgcaggc agaatgtact tttactaatc acgcgttcga
     4621 cagcctgatc ccgtcactga aattcagaaa atatgactcc gtaatctccg gtatggatat
     4681 cacgcccgag cgtagcaagc aggtatcgtt taccacgccc tactatgaaa actcagccgt
     4741 cgtgattgcc aaaaaagata cctacaaaac gtttgccgat ctgaaaggca aacgtattgg
     4801 gatggaaaac ggcaccacgc accagaaata tattcaggat cagcaccccg aagtgaaaac
     4861 tgtctcctat gacagttatc agaatgcctt tatcgatctg aaaaatggtc gtattgatgg
     4921 ggtatttggt gacacagcgg tggtaaacga atggctgaaa accaatccgc aactgggcgt
     4981 tgctactgaa aaagtgaccg atccgcaata cttcggcacc ggtctgggca tcgctgtacg
     5041 tccggataac aaagctctgc tggaaaaact gaataacgcg ctggcagcaa ttaaagctga
     5101 cggtacatat cagaaaatca atgaccagtg gttcccgcag taattgagac tgatgacaaa
     5161 agtaagattg cctgatgcgc tgcgcttatc tggcccacat caactctgca atgtattgat
     5221 ttagcatgat tttgtaggcc ggataaggcg tacacgccgc atccggcatg aacaaagcgc
     5281 acctgatcag caatctgaag ccgcacctgc ggctttttta ttgcttcacc agcagcgtca
     5341 tcacttcata gtgcgcggtg tgcgggaaca tatcaaaaag ctgtacccgt tcgatgcggt
     5401 aacctggcag ttcgcggata tctttcgcca tggtttgggc gttacagctg gagtagataa
     5461 taaaacgcgg tgccatcgtt gagagataat cacacagcgg tttaccaatg ccgcggcgcg
     5521 gcgggttaac cagcaccagc tccggcacat ccccctgagc ggtggcaaac tgagtggagt
     5581 ccagcgcctg aaattgcaaa cgcgttaagc ccagttcagc ggctgactgc tttgcacagg
     5641 caatggcctc tgatgcaatt tcgatcccgg ttaactgcat gtcaggcgtc gcgcagtgta
     5701 aaccaaagcc acccacaccg cagaacagat cccacatatg tttaactggc agctgccgta
     5761 cccagtcgcg cgcggtagcg tacagctggc tggcgaccgc cggattagtc tggaagaaac
     5821 tttgcggacg gatccacagc ggtacgtcat taaaacgctc tgccagcgcc tgttgttcag
     5881 tcaggtagat ctccgtctcc ccttccataa tcgccatatg taccggctga atattgacgg
     5941 taataacttt cagttgcggt agttgttcct gtaaccacgg cagcgcctta cgcagttgtg
     6001 ccagtttggt ttcagaacgc agtacaaagc gcagcatcat gccgccatcg ctctggcttt
     6061 cagtcagcag aatgtatttc agttcgccac gcttacgcgc cacgttgtag ggcgttaacc
     6121 ccgcacgggc gataaacggt tttagcgccg caaaaacggg cgcaaaagag gcaggataaa
     6181 gcgggcagtc acaaaggtct tccggcgtgc catcgcgatg cagcataccg agcagtggtt
     6241 tttcaacgct accactcacc accattttgg ctttattacg aaacccttgt tccgggcctg
     6301 acaccggcgc gcaccattcc tcaaccggaa aatcggcgag cagattttta agatcggcgg
     6361 ttttagcgga gagttgctct ggaatcggct gcgttatcca ctgacaggaa cgacagcgac
     6421 ccgcgtcgta aagtgcgcac tgcatacatt gaccttcaca tcatcagggg cgacgattat
     6481 acacattatt gcaactggaa gaaccgccga ctggtcgagg gaacgaacag cagcatcagg
     6541 atgagcatat ccggcagctt ctgcagcatc aggctatgga agatttcacg ttttgattca
     6601 ccgggaatgc tgaacagctc cggataaccg taccccagcg aagccgccca caagtaactt
     6661 gcggcggtga tttgtgtcag cagatatagc cagcgcgccc agcgacgccc tttcaccagt
     6721 gaaaacgcac accagatctc aatgaaaacc agcaccagac tgcttaaaaa gaccagcgtt
     6781 aaactccagg tttgtacgct gcgatgaatg aactcgccaa taccgcgcac gcccagcgta
     6841 ttgaaaatca tcagcacgtc gaggccacgg atcataataa tggcgagcgc cgccacctgc
     6901 accagcgcag gcacattcag gcgagcatga gatgaagatg ttttcttaaa aaatcccaac
     6961 gtttcgtctt ccatgaaaac aatgccgcgg catgcgcggc attatgtagc caggttggca
     7021 aattttagtg tcttcagcca tgtcttgcac gctggatatc gcgtatccgc tgtttttctg
     7081 cgcgagccat cagataccag gcgataaatc cgacaattcc gaccgcaccg agaattaacg
     7141 ttgcaagggc gttgatttcc ggattcactc ccatccgcac gctggaaaag accagcatcg
     7201 gtaacgtggt ggctcccggc ccggaaacaa agctggcgat caccagatca tcaagcgaca
     7261 aagtaaaagc cagtaaccag ccagaaatga tcgcgggcat gatcatcggt agcgtaatga
     7321 cgaaaaacac tttcagcggc gtcgcaccga gatccatcgc tgcttcttct atcgagcgat
     7381 ccagttcccg caatcttgaa gaaataacga ccgccacata agccgtacaa aaagtgacat
     7441 gcgccagcca gatggtgagc ataccgcggt ccgcaggcca gccaatggca tgagcaagcg
     7501 cgacgaataa taacaacagc gacaagcccg tgatgacatc tggcatcacc agcggcgcgg
     7561 tgatcataaa ggcaaaacca tttgatccgc gaaacctgcc aaaacgcacc agcaccaccg
     7621 ccgcaatagt cccgaggatc gccgccgccg ttgccgcaca ggcagcaatt gtcaggctta
     7681 aaccaaccgc actcatcatc gcgtcatcgc gcaataactc accataccag cgcgttgacc
     7741 agccggccca caccgtcacc agcttcgagc tgttaaacga atagataacc agcatcagca
     7801 ttggcgcgta gagaaaggta aagcccagca gcaaaatcac aatccgccag ggcgaacgaa
     7861 ctaccggtaa attattcatc cgtgttctcc cacgcttttt tgctggtgtt tgtggaacca
     7921 cattatcggc acaattagca gcagcaacat gatgatcgct accgccgagg ccaccggcca
     7981 gtcgcggtta ttgaaaaact cttgccataa cacgcgcccg atcatgatgc tgtccgggcc
     8041 accgagcagt tccgggatca caaactcgcc cacagccggg ataaacacca gcatcgatcc
     8101 ggcaataatc ccacctttag tcagcggcac gatcacagta aagaacgttt tcagcggtcg
     8161 tgcaccgaga tccagcgctg cttccaccag cgaataatca atacgaatca acgcggtata
     8221 aatcggcagt accataaacg gcacgtaagc gtaaacaatg ccgatataaa cggccagatt
     8281 ggtatgcaga atggtcagcg gttgatcgat aacccccagc cacagcagaa aattattcag
     8341 cacaccgttg ttttttaata ttcccatcca ggcataaacg cggatcagaa acgaggtcca
     8401 cgacggcagg atcaccagta gtaataaaat attacgggtc gaaggcttac tgtgcgccac
     8461 cgcccacgcc agcggatagc cgatcagtaa acagcaaaat gtcgaaatcg ccgccacctg
     8521 taacgactgg agataagcat caaaatagag cggatcgtcg gtcagttgca gaaaattacc
     8581 gagattaaga gtgatggaaa gttgcccgtc agcccactcc atcagttcgg tataaggtgg
     8641 aatagcgcgc gccatctctc ccaggcttat tttaaagaca atcagaaatg gcagcagaaa
     8701 cagcaagatc aaccagatat atagcaacgc aatgaccagt ttgcgcccat gcttcatttg
     8761 cagctgcgac aaccacagct taaatccgcc cggcggtttc gactgggcag caggttcaag
     8821 tgtactcatc tttgctcctt aaaccgtcag caccacacag ctgtccactt cccagcacaa
     8881 acgcacttcg tcgccccagg tcggtaaccc tttacgatga cgatgggcgt tttgtagctg
     8941 ggcgctgatc atctgcccac ttttcagtcg aacgtgatac accgaaagat cgccgagata
     9001 ggcaatgtgt atcacctccc ccaccgcgaa gttacaacca ttggcgggcg gctcttcgca
     9061 aagcatgatt ttttccgggc gcagcgctac gtgtaccggc acgttatcga ccactgaagc
     9121 atccgcatcg actttcagtg gatgcaccag ccccggcgaa tcaagcacca ggccatcttc
     9181 ctgacgctct ttgagtacac cttcaaagac attaaccgaa ccgataaatt cagcgctgta
     9241 gcgggtagtt ggatgctcgt agatctcttc cggctcgcca atctggacaa atttcccgcg
     9301 attcataatg gcgatacgcc ccgccatggt catcgcctct tcctgatcgt gggtgaccat
     9361 cacacaagtc acaccgacgc gctccagaat atccaccact tcaagctgca tccggtcacg
     9421 cagcttttta tccagcgcgc ccatcggctc atcgagcagt aatagtttcg ggcgtttggc
     9481 aaggcttcgg gccagggcca cacgctgtcg ctgaccaccg gaaagctgat gcggtttgcg
     9541 tttggcgaac tcctgcatgt gcaccaggcc gagcatctca tggacccgac tggcaatttc
     9601 cgctttcggt agtttgtcct gtttcaggcc aaaagcgatg ttctgttcca cggtcatatg
     9661 cggaaacagc gcgtaagact gaaacatcat attgatgggg cgcaggtaag gcggcacctg
     9721 tgacaaatcg acgccatcga gcattatctg cccggcagaa ggttgttcaa aacctgccag
     9781 catacgcagc agcgtggact tgccacagcc ggatgcgccc agcagcgcga agatttcacc
     9841 tttgtagatg gtcaggctga catcatcgac cgcatgctga ccatcgtagg atttggtgag
     9901 gttgcggatt tctaatagcg gcgtcagcgc cttacgggtt ttcgcctgcg ggcgagggat
     9961 agcgtcattc acggctgctc tccggcattg aatgtgtgca agcgcaccat gatggtgcga
    10021 attgccggct ggtgtggtgc gcccgcccgg catctaaggc tgcggattat tttccgctct
    10081 tcactttggt ccacgcgcgg gtgcgcacac ggtcgatttt cggatcctgt actttcagag
    10141 tgaatagctt cgcacgaacg tccgccggag gataaatacc cgggttctca cggacttccg
    10201 cactcaccag cggcgtggct gctttattgg cgttggcata gaacacatgg tcggaaatat
    10261 gcgctactac atccgggcgc agcaggtaat tcaggaactg ataggcttcg tctttgtttt
    10321 tggcatccgc aggcatggcg aatacatcaa agaacgccat cgccccttct tttggaatcg
    10381 agaacgagac attcactcca ttcttcgctt ccttcgcgcg gtttgacgcc tgccagacat
    10441 cacctgccca gccgatagcg acgcaaatat cgccgtttgc caggtcgtta atgtattgag
    10501 atgaatggaa ataacgaatg ttcgggcgca gctttaacag cagatcggtt gccggtccgg
    10561 tgtaatcatc cgctttagtg ctgttgggat ctttgccgag ataattcaac acggtagcaa
    10621 aaacttcttc tggcgcatcc aggaaagaga caccgcagct tttcagtttt tccagatttt
    10681 caggtttgag gatcaagtcc cagctatcga cgggcgcgtt ttcgcccagc acagctttaa
    10741 ctttatcaac gttatagcca atcccggtcg tcgcccacat atagggcata gcaaatttat
    10801 tgtcgggatc gtgtttggcg accagcttca gcagctccgg gtcgagattc ttccactccg
    10861 gcaatttgct tttgtccagc ggctggaaaa ctcccgcagt caactggcgt tcaagaaagc
    10921 tggcagatgg aaccaccaag tcaaagccgg tgctacccgc cattaatttg ccttccagta
    10981 cttcgttaga gtcgaaaaca tcgtagacga ctttaatacc ggtttctttt tcaaaattgg
    11041 ccaccgtgtc cggggcgata taatcagacc agttataaat gtggagtgtt ttttgttcag
    11101 ccgcgagcgt gccgacagag acggccatca gagcacccgc agccagaccc gaaagccatt
    11161 ttttatttaa ggcggtcata tctctttcct tctgaaagtt cgttaacaaa cgaacaaaag
    11221 ttgtcacact aaaagatatg caataaacgt gcatattttt tatcggcggg gtgtaagaaa
    11281 agagaactct ctcccggcca gcactgctcg ctatataaaa gcatcgcaag aataacttta
    11341 gccagggttt gagactatag ctcggattaa aaaacggtat ttaacaaatt tttatgcgta
    11401 tttgctctat gtgataaagc caaatcgaca caagagaagt gaaataatct ttaagaaaag
    11461 gttaaaaaca gcagataatt ttgcagcgcg cagccactgc accagcgact gcgtgctcga
    11521 aaggagttat cagtggagga aatagttttc ggtaacgttg ttttgccctt catcgtcagt
    11581 cggtaacagc agttgatggg cattagcttc aagaatgacc atcgaaatct gctcttcgct
    11641 ctgacgcacg aaccatgcaa actgctcata ggttacgccc tgcataacgg ataaagactg
    11701 gcaaaccacc agctttggca gattatcatc ctgcatatca agaaatgctt tcacggtcag
    11761 cgaactggca ttgatggcag ataaatccgc cgccagcggc aatacggctg aaggtcggac
    11821 ttccgccatg gcagaaaaca ggatcgtgtt atcgatcaga tcgattttgg catcaaagac
    11881 accgtcgaaa ttctgcatat ggggcagatg cagagcctga cagttatcac attcaaaaaa
    11941 actcatcccc aggtcatcaa gccattgacg cagcgtatcc agaccaggaa cgaccagcga
    12001 tgtcataatt ttgtacaacc tctttcgata aaaagaccgg cacagcttac gcaaaaagcg
    12061 caggcaaaac catgatcagt aatgtgattg cgactaacca cccgttttca ggcaatattc
    12121 tgtcgtagcg tggcgttcga tccagcggat cattttaccc gcgatgtcga taccagtggt
    12181 tttttctatt ccttccagcc ccggcgacgc attcacttcc atcaccaacg gcccgcgatt
    12241 agcacgcaga atatccacac cagcaacgtc cagcgccatc gttcgcgcgg cttttatcgc
    12301 gatttcacgc tcctgtggtg tgatactggc gacgcttgcc gcgccgccac gatgcaaatt
    12361 ggaacgaaaa tcgccctctt tcgcccgccg ttcaatcgca gcgaccactt catcgccaac
    12421 aaccagacag cggatatcgc acccttgcgc ctctttgata tattcctgca ccagaatatg
    12481 cgcgttcaga ccgcggaaag cgtcaatcac gctttccgcc gcctgacgcg tctccgccag
    12541 cacgacgcca attccctgcg tgccttcaac caacttgacc accagcggcg caccaccgac
    12601 catgtcgatt aaatcgctgg tatcatccgg cgaatgcgca atgcccgtga caggcaggtc
    12661 gatgccctga cgcgccagca gttgcatgga acgcaatttg tcacgcgccc gggcaatggc
    12721 gaccgactca ttgagcggat agctccccag catctcgaac tggcgcagtg ccgccgtccc
    12781 ataaaaggta atggcggtgc caatacgcgg gatcactgcg tcaaaatggg gtaacttgcg
    12841 gcctttgtag tgaatagaag acgccgcagg atttatgttc atgtagcaag aaagcggatc
    12901 aagaatttca accaggtgac cgcgctgtat agcggcttca cgcagccgct tacacgaata
    12961 gagcgttcca tcccgggaca atatggcaat tttcaccctg cacctctctg tcagacctgg
    13021 tcaaaagccc gcgtatcata catcgacgtg gcggttttag cgcgtcgccc aaccctgttt
    13081 gtgcaaataa tccagaataa atgggcggct ttctttaatg attgttcggc ggatatgatc
    13141 gctccaggta tcccggcgat tattgctgcc acgggtgagg taatattccg ccagttgctc
    13201 gtcatactgc gccagtgcgt ctttatccag cggttgatag ctgttttcat gcaccaaaat
    13261 ggaggccggt aaacgcggct tgagatccgg attatccgca ggccagccaa ggcacagccc
    13321 aaacagcggc agaacatgct gcggtaattt cagcagtttc gtcaccgctt caatattatt
    13381 gcgcaggccg ccgatatata ccccgcccaa tcccagcgat tccgctgcgg ttaatgcatt
    13441 ctgcgccatc attgccgtat caacgacacc gagcaacagt tgttccgcca ggccgagctg
    13501 agcatccgga cagatctgta aatggcggtt aaagtcggca cagaacaccc agaactccgc
    13561 cgcttgcgct acgtgttttt gcccgccggt cagcgtcacc agttcttcac gtaatgcttt
    13621 gtcggtaatg cgaataatgc tactacactg caaaaaactg gaactggacg tcgcacgggc
    13681 gctgttaata atcgcctcac actgcgcttc ggaaatgggt tcatcagtga aatggcgaat
    13741 ggagcgatgg ccacaaataa gttcaatggt tggcgtcatt atctttttct ctttctgaac
    13801 gtgaatattg cggtggacgg ttcatcagct gtggggcaag acgttttgcc acctgaagaa
    13861 taaccaccac cgcagcggga agcatgagca aaacaccgag aaaaatcatc agaatctgca
    13921 cttctggccg agaaaatggc tcaggcagcg acagggagtc gcttaccgac agcagcgcca
    13981 ccgccagtag catcattccg ataaattcca gtatcaacac gcctttaggc aatttaccga
    14041 tcgcgcgcat acgcttccct ctgcaaagtg agccttcagt ctaaaacttt tcactgtatt
    14101 gtgtttaaca gttatagctt ttagcaatta atgcaacagg ttaaacctac tttcagcgaa
    14161 tacattttag cgtgatcatt acaggcataa atctatgagg agagaaataa tgcaaaccgt
    14221 tatttttggt cgttcgggtt gcccttactg tgtgcgtgca aaagatctgg ctgagaaatt
    14281 gagcaatgaa cgcgatgatt ttcagtatca gtatgtagat attcgtgcgg aagggatcac
    14341 taaagaagat ctacaacaaa aggcaggtaa acccgtagaa accgtgccgc agatttttgt
    14401 cgatcagcaa catatcggcg gctataccga ttttgctgca tgggtgaaag aaaatctgga
    14461 cgcctgatcg tctgacaagc cctcgcgttg agggctttac tgattttttc tgtgctgtgg
    14521 tttaaacaaa ctactgataa ataagaaaca cagtgccccc agcgcacacc agaacaccgc
    14581 gcttagtaac catgccagct cttgccagaa tgagcgcgtc ggtgaaaaaa acagccgcat
    14641 aatgagcatt gaacagggtg ccgccagcat tgcgccaaat agaggtttca ggacttctct
    14701 acgctgtgaa aagaaactgg caactgctcc aggaagaatg aaaaacagca agccgatttc
    14761 aggatgcccg gcagcccgaa aagcgccttt catgtgcgtc gccagaaaaa ggcacaccac
    14821 aatgaagagg acaaaacagc agattgcccc cgcccaacgt tgtttatgtt tcactcgttc
    14881 ctcctgacac tgcgtctatc gaacacattt ttcgccagtg tggcgttcag taagataaag
    14941 ccgcttcgca ttccatgcta atataggcca acgcaattca tatagccgtt gatacctaat
    15001 gtgattacac tagtaaaata tattgttact ttactatcgt ttaggtgcgc tgaatgaatc
    15061 tgcgccctga attctggtaa aaaacattat cgtaaattac catttctttc aacagcttac
    15121 tagtaaacaa gaagttagcc tccgtgaata taaacgtcgc cgaattgtta aatgggaatt
    15181 acattctgtt attatttgtg gtcctcgcgc ttgggctatg tctcggaaag ttacgacttg
    15241 gttcgatcca actgggtaat tccattggcg ttttagtcgt atcgctgtta ttaggccaac
    15301 aacatttcag cattaacacc gatgcgctta atcttggctt tatgctgttt attttctgcg
    15361 tcggggtcga agccggaccg aacttttttt ccattttttt tcgcgatggg aaaaattacc
    15421 taatgttagc actggtgatg gttggcagtg cgctggtgat cgccttaggg ttaggtaagc
    15481 tgtttggctg ggatattggc ctgacggccg gtatgttagc aggctctatg acgtcgacac
    15541 cggttctggt cggtgctggc gatacactgc gtcattccgg catggaaagc aggcagctct
    15601 cactggcact ggataatctg agcctcgggt atgccttaac ctatttaatc ggtctggtga
    15661 gtttgattgt tggtgcgcgt tacttgccga aattgcagca tcaggactta cagaccagcg
    15721 cccagcaaat cgcccgcgaa cgtggcctgg acactgatgc caaccgtaag gtttatttac
    15781 cggtgatccg cgcctatcgc gtcggcccgg agctggtggc ctggaccgac ggcaaaaatc
    15841 tgcgtgaact gggtatttat cgacaaaccg gctgctacat tgaacgtatt cgacgtaacg
    15901 ggattctggc aaatccagac ggtgatgccg tgctacaaat gggcgatgaa atagcgttgg
    15961 taggctatcc cgacgcccat gcccgactcg atcccagctt ccgtaacggt aaagaagttt
    16021 tcgatcgtga ccttctcgac atgcgtatcg tcactgaaga agtggtcgtt aaaaaccata
    16081 acgctgtagg taaacgtctc gcacaactga agttgaccga tcacggttgc ttccttaacc
    16141 gcgtcattcg tagccagatt gagatgccga tagatgacaa cgtcgtgctt aacaaaggtg
    16201 acgttttaca agtcagcggt gatgcccgtc gcgtaaaaac catcgccgat cgcatcggct
    16261 ttatctcgat tcacagccag gtcactgacc tgctggcatt ttgcgccttc tttgttattg
    16321 ggctgatgat cgggatgatc accttccagt tcagcacatt cagtttcggc atggggaacg
    16381 ctgccgggtt gttattcgcc ggaattatgc tgggctttat gcgtgctaac cacccgacct
    16441 tcggttacat tccgcaaggt gcattaagca tggtgaaaga gttcggcttg atggtgttta
    16501 tggcaggcgt tggtctgagc gccggtagcg gtattaataa cggcctgggc gcgattggcg
    16561 gtcagatgtt gattgccgga ttaattgtca gtcttgtgcc cgtggttatc tgtttcttgt
    16621 tcggtgctta tgtattgcga atgaaccgcg cactgttgtt cggcgcaatg atgggcgcac
    16681 gcacctgcgc gccggcaatg gagatcatca gtgatacagc tcgcagtaac atccctgcgc
    16741 tgggctatgc gggcacctac gcaatcgcca acgtcctgct gacgctggca gggacaatca
    16801 tcgtcatggt atggccagga ttaggataaa actgaagttg ccctgaaaat gaaatttttt
    16861 tgcacaaccg cagaactttt ccgcagggca tcagtcttaa ttagtgccac tgcttttctt
    16921 tgatgtcccc attttgtgga gcccatcaac cccgccattt cggttcaagg ttgatgggtt
    16981 ttttgttatc taaaatttat ctactaccct gcaaccctct caaccatcct caaaatctcc
    17041 tcgcgcgata gcggcttacg gtcggtgaca aaatgcagcg tcatcccctc aataaacgca
    17101 tcaagcgcgc gggcggttcc gggttcaaac cattgttcga gcgtttgctg actgcgctgc
    17161 atccagtttt gcattaccgt ttttaatagc ggttttcggc tagccagcgc gtagagctgg
    17221 tacatcagct ccatattatc cggcgttgca acctgtgagc tgtagatcat atcggtgata
    17281 gcctggcatg cgccttgagc atcactaaca tcgctaaaaa atgcctgata ttgccgggac
    17341 ataatctcag taaaactgct gaacgcctcc agcaacaact catcaattcc tgaaaaatag
    17401 taggtcatcg atcccaacgg taccccggca agggtagcaa ttttgcggtg cgtaacagca
    17461 tgtattccgt aaagtttcac cgcctccagc gtggcctgga taattttttc tcgccgttgc
    17521 ggatcgttag cgcgacgcat gtcatttcct ctgtctgcaa ttgtgtacaa atgtacacaa
    17581 ccttgctaat gttgtcttcc ctcttcttat tttgacgtga tctatgaccg taaattcttc
    17641 acgtaatgca ttgaaacgcc gaacctgggc gctgtttatg ttcttctttt tgccaggcct
    17701 gttaatggcg tcctgggcaa cccgtacgcc tgctatccgc gatattctct ctgtctcgat
    17761 cgctgaaatg ggcggtgttc tctttggtct gtcgatcggt tcgatgagcg gtattctctg
    17821 ctcggcgtgg ttagtgaaac gctttgggac acgtaatgtc atcctggtca cgatgtcctg
    17881 cgcattgatc gggatgatga tattaagtct ggcactctgg ctgacatcgc ccctgctctt
    17941 tgccgttggt ctcggcgtct ttggggcaag ttttggttct gcggaagtgg cgataaacgt
    18001 tgaaggtgcc gccgttgagc gagaaatgaa taaaacggtt ttgccgatga tgcacggttt
    18061 ttatagccta ggcacgctgg caggcgctgg tgtcgggatg gcactgacgg cctttggcgt
    18121 tccggcaacg gtgcacattt tattggcggc gctggtaggt atcgcgccta tttatatcgc
    18181 cattcaggca atccctgacg gtacgggcaa aaatgctgcc gatggcaccc agcatggcga
    18241 aaaaggcatt cctttttatc gcgatatcca gttgctgttg attggtgttg tagtgctggc
    18301 gatggccttt gccgaaggtt ctgccaacga ctggttaccc ttattaatgg ttgacggtca
    18361 cggttttagc cctacttccg gctcgctgat ttatgccggt tttaccctag ggatgaccgt
    18421 tggacgcttc actggcggtt ggttcatcga ccgttacagt cgcgttgccg tggttcgggc
    18481 cagtgcacta atgggggcgt tgggtattgg gctgattatt tttgtcgata gcgcctgggt
    18541 cgctggggtg tctgttgtac tctggggact gggtgcctcg ctgggcttcc cgctgaccat
    18601 ttctgccgcc agcgataccg gccccgatgc accaacccgc gtcagtgtgg tagcaacgac
    18661 cggttatctg gctttcctcg tcgggccgcc gctgctgggc tatctcggcg aacattatgg
    18721 attacgtagt gcaatgctgg ttgtactggc gctggttatt ctcgcggcta ttgtcgcgaa
    18781 agccgtcgcc aaacccgata ccaaaacgcg gacggcgatg gagaatagtt gatgagcatt
    18841 aaattaattg cggtagacat ggatggtact ttcttaagcg atcaaaaaac ctataaccgt
    18901 gagcggttta tggctcagta tcagcaaatg aaagcacaag gcattcgctt tgtggtcgcc
    18961 agcgggaatc aatattatca gttgctctct ttcttccctg aaattgctaa tgaaattgcc
    19021 tttgtggctg aaaacggcgg ctgggtagtg agtgaaggca aagatgtttt taatggcgag
    19081 ctgtcgaagg atgcctttac tactgtcgtg gaacatttgc tgacgcgccc ggaagtggaa
    19141 attattgcct gcggaaaaaa tagcgcctat acgctcaaaa aatatgacga tgccatgaaa
    19201 acggtggcag aaatgtatta tcaccgtctg gaatacgtcg ataactttga caacttagaa
    19261 gatattttct ttaagtttgg cctgaatctt tccgatgaac tgattccaca agtacaaaaa
    19321 gcattacatg aggccatcgg cgatattatg gtgccagtcc acaccggcaa cggcagcatc
    19381 gatctgatta tccccggcgt acataaagcc aatggccttc gccaactgca gaaattatgg
    19441 ggaatagacg acagcgaagt ggtggtcttt ggcgatggcg gtaacgatat tgagatgctg
    19501 cgtcaggcag gctttagttt tgcaatggaa aatgccggca gcgcggtcgt cgcagcggca
    19561 aaataccggg caggctccaa taaccgtgaa ggcgtactgg atgtgatcga taaagtgctt
    19621 aaacacgaag cgccatttga ccaataaaaa cggcagcacg cccggcaaca caatacctga
    19681 atgaaggctg cataattcgc ggaaattaac ccgctgcatt atttcaggcg aaatgattat
    19741 gaaaaattgt ctactgttgg gcgcgctttt aatgggcttt actggtgtgg cgatggcgca
    19801 aagtgtcacc ggcgatgtgc cgagtggata caaagtggtt gtagttcctg attcagtcag
    19861 cgttccacaa gcggtcagcg tcgcgactgt gccgcaaacc gtctatgtgg ctcctgcgcc
    19921 agctccggct taccgcccgc atccttatgc tcgccatctt gccagcgtag gtgaagggat
    19981 ggttatcgaa caccagattg acgaccatca ccattaattc gagaatgcct gatcgcacaa
    20041 agcagtcagg cattttttta cccttcgtga gaatttccca tctgtttatc ttttaaaaag
    20101 ataaccatca gcgacagcca caaaattccg ttgacaaggt tgaagagatt aaactgtccg
    20161 ttgcccccgt tcagccaggc atgtttgctg atttcaatac caacggtaaa gatcagcatt
    20221 tgcagcattc ccatcgccgc agaaaccgta cctttactca tatcgctggc aaacagggtt
    20281 aatcgcacca gtcccgcatt cgccagacca ataccgaaag cataaatact taacccggca
    20341 gtcatccata aatacgcgtg cgatgagata accgttgccg cagcagcgac caacagacca
    20401 atcataatcg gccagccgcc cataattatc acgtacggtg cggcgcgagg tcagacgcgc
    20461 taacagcaag ttacccgcaa ttaacgcccc gaaaataggc acttgcagca agccatattc
    20521 atagctgctc aactgctccc cggtaatgat gataatcggc gactgggcga tccacgccag
    20581 caatggcagg ctaacgaatc ccagcgccag cgcccccgcc acaaagcggc cgttcttcag
    20641 caccagctta tagtcacgac cgagttcttt cagtgacagt ttctcgccta tacgcgtggc
    20701 ggtttcaggc atagctcgtt gcagaccgaa aaaggagatc gctgccaatg cggcaaacaa
    20761 aacaaacatc ccctcccagg gcagcacatg gatccacgcc gcgcccacca gcggaccaag
    20821 tagcggagca atcagcgcca cgttcgccat cagcgcggtg atcttgatac aaaccgcctc
    20881 ttcgaaggat tcctgaattg cggcgtatcc cacagcgcca atgaaacaga ggcttatgcc
    20941 ctgcaagaag cgcaacaggg tgaattgttc aatgttttgc gccagtaata ttgccagaca
    21001 ggtgacgata aaccacacca cacccgccag catcaccgga cggcgaccaa tacgatccga
    21061 cagcggcccc aggagccatt gtaaaaacat cccgcccgcc agatacgcgg tcatcgaagt
    21121 aggaacccaa tcaatgcccg cctgatattg ttccaccacg gccaacatac cgggttggat
    21181 catatcgttg ccgatatagg ttgaaaattc gtaaagcacc agacagagag ggaaaagtaa
    21241 cgcctgacgt ccaagcctgg caccgaaagc taatttattt tgcatgcaat ttcttcgcca
    21301 ataataatcg cgcagagttt aataaaagcg cagctaacga gaaagcgaat tttgtagctg
    21361 aaaccacggt taagcacatt cttacattat tgcgagtata gctacgcttt ctttaagttt
    21421 tatttaacct atgcccgtta caatcaccca ccgtaaacag gccgcttgag ggaaataaga
    21481 cgatgccgct ttacccagtt taacctgcac tttattctca acgacttgcc tgtattggct
    21541 cccttttaat cactttgcgt cgggaagtta tgctggaaaa tttgaatctc tctctattct
    21601 ctcttattaa cgcgacgcca gactcggctc cgtggatgat ctcgttggcg atttttattg
    21661 ctaaagattt gattaccgtg gtgccgttgc tggccgcggt actttggttg tgggggctta
    21721 cagcacaacg gcaactggtg ataaaaatcg ctatcgcgct ggcggtcagc ctgtttgtgt
    21781 cctggacgat gggacatctt tttccgcacg accgaccctt tgtcgaaaat atcggctata
    21841 acttcctgca tcatgcggcg gatgactcat tcccgagcga tcacggtacg gtgattttca
    21901 cctttgcact ggcattttta tgctggcatc gcctgtggtc cggctcactt ttaatggttc
    21961 tggccgtcgt cattgcctgg tcgcgcgttt atcttggcgt ccactggccg ctggatatgc
    22021 tcggtggatt gctggcaggt atgattggct gccttagtgc ccagattatc tggcaagcga
    22081 tggggcataa actctatcaa cgtctgcaat cgtggtatcg cgtctgtttt gcattaccga
    22141 tccgcaaagg ctgggtgcgt gactgatctc tgctaaaaag tgtagtattg agcggctcgc
    22201 ttcaataact attcagaggg attatggaaa cacgtcgcga agagcgtatc gggcagctgc
    22261 tgcaagaatt aaaacgcagc gataagttac atcttaaaga cgccgccgcc ctgcttgggg
    22321 tttcggagat gacgattcgt cgcgatctga acaaccacag tgcgcccgtc gttttgctcg
    22381 gcggctatat tgttctggaa ccgcgcagtg ccagccatta cctgttaagc gatcaaaaat
    22441 cccgcctggt ggaagaaaaa cgccgggcgg caaaactggc tgcgacactg gtagaacccg
    22501 atcagaccct cttttttgac tgtggcacca ccacgccgtg gattattgaa gcgattgata
    22561 atgaaatccc ttttaccgcc gtttgttatt cgctaaatac ctttctggcg ctgaaagaga
    22621 aaccccattg ccgcgcattt ctgtgcggtg gtgaatttca cgccagcaac gccattttca
    22681 accccatcga ttttcagcaa acgttgaata atttttgccc ggatatcgct ttttattccg
    22741 cggcgggcgt gcatgtcagt aaaggcgcta cctgttttaa tcttgaagag ttgccggtaa
    22801 aacactgggc catgtcgatg gcgcaaaagc atgtgctggt tgtcgaccac agtaaatttg
    22861 gcaaggtgcg tccggcgcgc atgggtgacc tgaaacgctt tgatattgtg gtgagtgatt
    22921 gttgcccgga agatgagtat gtgaagtacg cgcagacgca ccgtattaag ttgatgtatt
    22981 aatgacgtat aaccggatga cgtttcgcgc catccggtta tcagaagatt aagagaacca
    23041 gctgccgaac cactgatgga atttcatcat cacgaaatcc cacatccgac caaagaatcc
    23101 gccctcttcc acattttcca tcacgatcag cggacgctgc tcaatggatt taccgttaag
    23161 ctggaaatca atggtcccga caacctgacc ttttttcagc ggtgcggtaa gctgcggttc
    23221 cgttaacgtg tagctcgcct tcaggttttt cagctgccca cgcggtatgg tcacggagcc
    23281 cgcttcgcct gccccgagat tcacttcgct cttatcacca aaccagacgc gctgagtcac
    23341 aaaggtggca tcaggtttaa ttggcgtcac agtttcaaag aagcggaaac cccaggtcaa
    23401 taatttctca gactcattaa aacggatacg gtcagttttc gcccccagca ctacggagat
    23461 taaacgcata tcaccctggg tagccgaagc aaccagatta tatcccgcac ctgccgtggt
    23521 tcctgtcttc atgccatcaa cattcagatt gctgctccac agcagacggt tacggttagg
    23581 ctgacgaatt ttgttgaagg tgaactcttt ctctttatga atggcgtact cttccggcac
    23641 atcgtggatc aatgctttac ccagcaatgc catatcgcgc gcggtactga actgccccgg
    23701 cgcatccaga ccgtgcaccg tctggaaggt agtgttggtc agacccagtt ttttggcata
    23761 accattcatc aaaccaataa atgactcctg gctcccggca acgtaatcgg ccagcgcaat
    23821 acaggcgtca ttaccggact ggataatcac acctttgttc aagtctgcca ccgaaacctg
    23881 atcgcccggt ttgaggaaca ttaccgatga accacgcagt gccggatttc ccgtcgccca
    23941 ggcatcttta ccgaccgtca ccatatcggt gagtttaatc ttatcggcct taagcgcctg
    24001 cccaaccaca tagctggtca tgattttagt caggctcgcg ggatccagtt tctcatccgc
    24061 gttgccttct gccagcactt taccgctggc gtaatccatt aaaatccatg cacgcgcatc
    24121 cacgctcggc gcttcaacgg tttgttccgc cgcgaatgcc gttggggcaa aaaggaataa
    24181 aaatgcagaa cccgctgcaa gaccacgaag gagagaggag tattgcgtca taaatgccac
    24241 ccgagtatcc attcaaaaaa aatacgttat atcgccgtat gataagttcg gtgattaata
    24301 acgcacacac aaacttaaag aaaccgtaag ttggtaaagt ttttaaagtt tatgcaatat
    24361 cctgccgctt cgctgcttct ggtgtcattt ttttgccagc tgttgcttaa aagttaactt
    24421 tacttcacca tcggagtcct gttcagcctg tggctgcctg tcggaaaagg ggtgagtatg
    24481 attacgctgt ggggtcggaa taattcaact aacgtaaaaa agtattgctg acgctcgaag
    24541 aactggaact accttatgag caaattctcg cgggccgtga gtttgggata aatcacgatg
    24601 ctgattttct ggcgatgaac cctaacgggc tggtgccgtt gttgcgtgac gacgaaagtg
    24661 atctcattct ttgggaatca aacgccattg tccgctatct ggcggcacag tacgggcaaa
    24721 aacgcctgtg gatcgactca ccggcacgtc gtgcggaagc ggaaaaatgg atggactggg
    24781 caaaccagac gctcagtaat gctcatcgcg ggatcctgat gggattagtc agaacaccac
    24841 cggaagagcg cgatcaggcc gccattgatg ccagttgcaa agagtgcgac gccctgtttg
    24901 ccctgctcga tgcggaactg gcaaaagtaa aatggttctc tggcgacgag tttggtgtgg
    24961 gcgatatcgc tatcgcaccg ttcatctaca atttgtttaa cgttggcctg acctggacac
    25021 cgcgtccaaa tctgcaacgc tggtatcagc aactcactga acgccccgca gtgcgcaaag
    25081 tggtgatgat tcccgttagc taattgcgtg ggctaacttt aagtaattcc ccactggact
    25141 cgtcggtgag aacgtataaa taaccgtcgg gtccagtgcg aacatcacga attcgctgcc
    25201 ctctgtccgt taaaatacgg ccatcttctg tcactttgtc gccgttgacg ctcatcacaa
    25261 tgacatcttt atctttcagc gcgccaataa ataatttttg ctgccactgg gggaatttat
    25321 cgctgttata gaaggccatg ccgctcacag cgggcgaatc tttccagtaa aaaacaggtt
    25381 gctcggtccc ggcgacgatc tcccctttcg cttccggtat cttaaagcct gaatagttga
    25441 ttccccaggt tgccagcggc cagccgtagt ttttgccttt ttgcgggata ttaatttcat
    25501 caccaccgcg cgggccatgt tcattcagcc acagtgcatt actccacgga ttcatcgcca
    25561 ttccttgcgg attacgaatg ccataagacc agatctcggc gcgcgcaccg gattccttta
    25621 taaaaggatt atcatccggg atttcgccct ggtcggtcag acgcaccagt ttgccctgta
    25681 atttatccag atcctgcgcc gtcgggcgct gattgttttc gcccagagca ataaaaagat
    25741 aacctttacc gtcgaatacc agccgcccgc caaaatggtt gccggtagac agttttggca
    25801 tctggcggaa gacggtgcgg aagtcggtca cttttgagag atcatcactt aagcggccat
    25861 aacccacagc agttccggct ttgccatcat cgccaacttc ggaataactt aaccagatgc
    25921 ggcgagactg agcaaaatca ggcgctaaaa ccacgtccag caggccgccc tgcccgtgcg
    25981 cccaaacgtc cggaactccg gaaagcggcg cagataatcc ttttcctgct tgccagtgac
    26041 gcaactcgcc gccgcgcaga gtgattaaca taccgtgatt atcgggtaaa aaggccagtg
    26101 cccagggatg gtcgagtttg tcttgcagta cttcgacatt taccgttgca ggagccgccc
    26161 agagagcgga agaaagacaa ataaggggca caaggaaaaa ggattgtcga tgcataacgc
    26221 ctccggattg actctggctt aaagcgtagt cagtggagga gataacaaat tcatttttac
    26281 aaaaacttaa acatgaaggg ggagacgctt tctccccctt agttttcagg ccttctcaag
    26341 catggcgtgc ttctgcaggc tctggatact cagcgttaag ctcatcagac aattttcaag
    26401 cttatcggcg ttgacggtaa taacagtcgg gcaatcatgg tgcccactca tcaaacatac
    26461 tgcggctgtc gctaatgctt cttcagcatg atgaagagca ctccactctt cctgatccag
    26521 atgaagattc aaccgcagcg atttatcgtg cagttcgcga ttcagtttaa aaaagttatc
    26581 tcgtagatga ttgctttcgc tgacggacat gtatcctttt gccttcctca gttgcacgta
    26641 tgcagcgagg tcaacgcgtg cgtcgatcaa ccggttcagc agatcgattc gctgtctgtc
    26701 aacgaacata cttcgatcct cctctttcca gccttgttgc gattaaatag tatggctatt
    26761 ttttgagcaa aatgctgtcc aggatcaatt cttcacgcca aattaacaaa atttacattt
    26821 ccttcgcctc cgccagcgaa tgagattgcg atacaaattt tatccagaac tctgtagaat
    26881 ccctgccctg acctttcctg taactgaata ttttttaagc tatgagcaaa gtaactcccc
    26941 agccgaaaat cggctttgtt tcccttggct gtccgaaaaa ccttgtcgat tcagagcgta
    27001 ttctcaccga actacgcact gaaggttatg acgtggtacc gagctatgac gatgcggaca
    27061 tggtgatcgt caacacctgc ggctttattg acagcgcggt acaagaatca ctggaagcca
    27121 ttggtgaagc gttgaatgaa aacggcaagg taattgtgac cggttgtctg ggggcaaaag
    27181 aagatcagat ccgcgaagtc cacccgaaag tgctggaaat caccgggcct catagctatg
    27241 agcaggttct ggagcacgtt catcactacg tgccaaaacc gaaacacaac ccattcctga
    27301 gcctggtgcc agaacaaggt gtgaaactga caccgcgtca ttatgcctat ctgaaaattt
    27361 cggaaggctg taatcaccgc tgcaccttct gcattattcc gtctatgcgc ggcgacctgg
    27421 tgagccgtcc gattggcgaa gtgttaagtg aagcgaaacg tctggtagat gcgggcgtta
    27481 aagagattct ggtgatctcg caggatactt ccgcctatgg cgttgatgtt aaacatcgta
    27541 ctggcttcca caacggcgag ccggtaaaaa ccagcatggt cagcctgtgc gaacagttat
    27601 cgaaactggg gatctggaca cgtctgcact acgtttaccc ttatccgcat gtggacgacg
    27661 tcatcccact gatggcagaa ggcaaaatcc tgccgtatct ggacattccg ttgcagcacg
    27721 ccagcccgcg cattctcaaa ctgatgaagc gtccgggttc tgtagatcgc caactggcgc
    27781 gcatcaaaca gtggcgcgaa atctgcccgg aactgaccct acgctcaacc tttattgtcg
    27841 gcttccctag cgagacggaa gaagatttcc agatgctact cgacttcctg aaagaagcgc
    27901 gtctggatcg cgttggctgc tttaaataca gcccggttga aggtgcagac gccaatgccc
    27961 tgcctgacca ggttccggaa gaagtgaaag aagaacgctg gaaccgtttc atgcagctgc
    28021 agcagcagat ttccgccgag cgcctgcaag agaaagtggg ccgtgaaatt ctggtgatta
    28081 tcgacgaagt ggacgaagaa ggcgcgattg gtcgcagcat ggcagatgca ccggaaatcg
    28141 acggcgcggt ttatctcaat ggtgaaacca acgttaagcc gggtgatatc ctgcgtgtga
    28201 aagtcgagca cgccgatgag tacgatttgt ggggtagccg ggtttaatta attcaggccg
    28261 ggcaaaatca tttgtccgcc ctgcgaataa tgattactgc cagctaatct gagccctgcc
    28321 gtttttctta ctcttataca gccgctcatc cgccagcatt ttcaagcgtt cgacggtggt
    28381 acattcattt gaatgcgcta caccgatact ggtacttaaa ttaatgcttc ccacctggaa
    28441 atgaacgatt tcttttagaa tcgatagcaa ctcttccagc gttccacccg tataaatcac
    28501 cgcaaattcg tcaccgccga agcggtagat tttaccttta tctaccggga tctgtgattt
    28561 aaggattttc accactttca tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt
    28621 aatgtttttg aatttatcga cgtcaaacag gcacaaataa ccgccttttt ccggcagcgc
    28681 ttttaaatct tcgtcaaagg attttctgcc cattgctttg gtcaggtcgt ccaggaacgc
    28741 cgagttatac atactgtaga gttttttgac atcggcagcc gaaaagaaaa gcccgccatc
    28801 cagtacgcgt tcaaaatggt tattaatgat ggcttctttc tccacaatac cacgattaat
    28861 cactctgcgt accagataga ggcaactggt acccgatatc actgccacca atgcggcaaa
    28921 gaaaataatc aatgtacaga cccgcaaata aacatctttt ttatacgcaa atacgtacaa
    28981 tccggtcggc aaataggcga agtgctccat cgcttccgaa acgatgaaga tggcgttttt
    29041 agcattatat tcatcaccgg tttaccgttc ttaaatagag agaacatcag gttctcgctg
    29101 tccatcgcca gcgattttga cagtaaagaa aaacgctcca gaccaagcaa aaatatcaga
    29161 ctacgattgt gagttaccgg atagaggtaa tagacgaagg ttttaccgtc aaaagagata
    29221 aaccctgtat ttttgacatg tcgatacttc acaacctgca taatatctgc acaataattt
    29281 ttctcgaact cctttacctg aatacattcg tcactggaaa tcagataagg accgacatcg
    29341 tacaaataag agggggaaac cgagtcggcg taagctgtgc gcaaaccaac caacagctgt
    29401 tcctccgtgg gatttagcgt gttgataaga tcaattgcca cgctacgccg atattcagca
    29461 atatttgcca ccgcaaaagc agtatcaata acggactgtt tcaggctata cacccgttcc
    29521 tttaccattt gagtgtagat cccgcaggca actgctgaaa tcatgataaa aatcagcaga
    29581 ctgactgtaa gtacgaactt attgattctg gacatacgta aactactctt ttactaattt
    29641 tccactttta tcccaggcgg agaatggcat gggtttctgg tacagatacc cctgagcctg
    29701 aacacatcct aatttaatta attcgcgcgc aacgtcttcg ctctctacgc cttccgcaat
    29761 caccgggatc cggttatagc gtgccagatc gaaaataatg cgcacaattt ttcccgcagt
    29821 accgaaaata tcgccggtaa attgcgagtc cacctttatg ctgtctggat taatttcata
    29881 aaaacgtttc aggtttgaca aaccggtgcc aaagtcatca atcgatatcc gtaccccttg
    29941 tttacgcagt tgttcaatat tgagataaaa atgttcatta attcgctcga tcacggtttc
    30001 agtgatctca acacagaggc tgttcgcctt caggccattt tctttcatga tggtggtaaa
    30061 acgctcatga aagtgcggtt cgtttaactg ccagggcgaa acattaatat gcagtaagaa
    30121 atcatccttc agcgacgcac gctgactaaa atgtctgaat tcacccattg ccgttttaat
    30181 aatctgataa cccagctcat tgataaaccc gatatcttca gcaatggtaa tgaacttcag
    30241 cggtgaaatg atcccccgct ccgcagaaac ccaacggcac aacgcctccg cgcccagaat
    30301 ggcgcgggtg ttaatatcaa cgatcggttg atagacgagg aatagttcgc caatttccat
    30361 tgcatggcga atattacgcg caatccagat attctcttct atttcgttga gtttcgcgtc
    30421 atcacagaaa ataaactcgg agaacatatg gtcacggata ctggaaatcg ccagtttcag
    30481 tttttctgcg taactccagg cgttttcccc ctgcaaaggt tcacaaatgg cagacttatg
    30541 gatgcgcaga ttgcggtagc caaagctgct gtcaaaatcg tttaccatcg ctattttacg
    30601 ggtaaatgtc tgtacatttt cccgacacag aagtaaatag agatcggcat tgaatgtcac
    30661 gatatcgcag cattgcgcat atttctcggc aattcgctga cacaatttcc tgacgatttt
    30721 attgcgttca tcaacaccaa acaccgcttc cacggcttcc agaccacaca gtttgatgat
    30781 ccccacagcc agatactgat tattgttgga ctcaataatc gccatatttt taataaaacc
    30841 gctttcctga tcgtaagaga tcagtgagag caatttattt gaccgttcaa tttcggcatc
    30901 tttcatttcc gcgatcgaat t
//
LOCUS       DAZPBM010000032        30154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPBM010000032 DAZPBM010000000
VERSION     DAZPBM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.032"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHUZQ_001356"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHUZQ_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR1025090.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHUZQ_001357"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHUZQ_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1025091.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHUZQ_001358"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHUZQ_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1025092.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUZQ_001359"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUZQ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1025093.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUZQ_001360"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUZQ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1025094.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUZQ_001361"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUZQ_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1025095.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUZQ_001362"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUZQ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1025096.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUZQ_001363"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUZQ_001363"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1025097.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUZQ_001364"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUZQ_001364"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1025098.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUZQ_001365"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUZQ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1025099.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUZQ_001366"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUZQ_001366"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1025100.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUZQ_001367"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUZQ_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1025101.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUZQ_001368"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUZQ_001368"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1025102.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUZQ_001369"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUZQ_001369"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1025103.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUZQ_001370"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUZQ_001370"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1025104.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="ACHUZQ_001371"
     CDS             complement(18516..18992)
                     /locus_tag="ACHUZQ_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR1025105.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUZQ_001372"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUZQ_001372"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1025106.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPLSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUZQ_001373"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUZQ_001373"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1025107.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUZQ_001374"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUZQ_001374"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1025108.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHUZQ_001375"
     CDS             21688..22278
                     /locus_tag="ACHUZQ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1025109.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUZQ_001376"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUZQ_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1025110.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHUZQ_001377"
     CDS             22750..23445
                     /locus_tag="ACHUZQ_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1025111.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUZQ_001378"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUZQ_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1025112.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUZQ_001379"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUZQ_001379"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1025113.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUZQ_001380"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUZQ_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1025114.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUZQ_001381"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUZQ_001381"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1025115.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUZQ_001382"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUZQ_001382"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1025116.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7056 a         7867 c         8307 g         6924 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tccttgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctctg
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaag
//
LOCUS       DAZPBM010000033        30084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPBM010000033 DAZPBM010000000
VERSION     DAZPBM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.033"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            194..856
                     /gene="acrS"
                     /locus_tag="ACHUZQ_001383"
     CDS             194..856
                     /gene="acrS"
                     /locus_tag="ACHUZQ_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR1025117.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(859..1038)
                     /locus_tag="ACHUZQ_001384"
     CDS             complement(859..1038)
                     /locus_tag="ACHUZQ_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1025118.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(1122..2006)
                     /gene="yhdJ"
                     /locus_tag="ACHUZQ_001385"
     CDS             complement(1122..2006)
                     /gene="yhdJ"
                     /locus_tag="ACHUZQ_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1025119.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(2092..2388)
                     /gene="fis"
                     /locus_tag="ACHUZQ_001386"
     CDS             complement(2092..2388)
                     /gene="fis"
                     /locus_tag="ACHUZQ_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1025120.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2414..3379)
                     /gene="dusB"
                     /locus_tag="ACHUZQ_001387"
     CDS             complement(2414..3379)
                     /gene="dusB"
                     /locus_tag="ACHUZQ_001387"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1025121.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3708..4589)
                     /gene="prmA"
                     /locus_tag="ACHUZQ_001388"
     CDS             complement(3708..4589)
                     /gene="prmA"
                     /locus_tag="ACHUZQ_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1025122.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4601..6052)
                     /gene="panF"
                     /locus_tag="ACHUZQ_001389"
     CDS             complement(4601..6052)
                     /gene="panF"
                     /locus_tag="ACHUZQ_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1025123.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(6042..6284)
                     /locus_tag="ACHUZQ_001390"
     CDS             complement(6042..6284)
                     /locus_tag="ACHUZQ_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1025124.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6393..7742)
                     /gene="accC"
                     /locus_tag="ACHUZQ_001391"
     CDS             complement(6393..7742)
                     /gene="accC"
                     /locus_tag="ACHUZQ_001391"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1025125.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7753..8223)
                     /gene="accB"
                     /locus_tag="ACHUZQ_001392"
     CDS             complement(7753..8223)
                     /gene="accB"
                     /locus_tag="ACHUZQ_001392"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1025126.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9201..10175)
                     /locus_tag="ACHUZQ_001393"
     CDS             complement(9201..10175)
                     /locus_tag="ACHUZQ_001393"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1025127.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10327..12267
                     /gene="csrD"
                     /locus_tag="ACHUZQ_001394"
     CDS             10327..12267
                     /gene="csrD"
                     /locus_tag="ACHUZQ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1025128.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12572..13615
                     /gene="mreB"
                     /locus_tag="ACHUZQ_001395"
     CDS             12572..13615
                     /gene="mreB"
                     /locus_tag="ACHUZQ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1025129.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13681..14784
                     /gene="mreC"
                     /locus_tag="ACHUZQ_001396"
     CDS             13681..14784
                     /gene="mreC"
                     /locus_tag="ACHUZQ_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1025130.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14784..15272
                     /gene="mreD"
                     /locus_tag="ACHUZQ_001397"
     CDS             14784..15272
                     /gene="mreD"
                     /locus_tag="ACHUZQ_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1025131.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15281..15874
                     /gene="yhdE"
                     /locus_tag="ACHUZQ_001398"
     CDS             15281..15874
                     /gene="yhdE"
                     /locus_tag="ACHUZQ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1025132.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15864..17333
                     /gene="rng"
                     /locus_tag="ACHUZQ_001399"
     CDS             15864..17333
                     /gene="rng"
                     /locus_tag="ACHUZQ_001399"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1025133.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17401..21201
                     /gene="yhdP"
                     /locus_tag="ACHUZQ_001400"
     CDS             17401..21201
                     /gene="yhdP"
                     /locus_tag="ACHUZQ_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1025134.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21357..22802
                     /gene="tldD"
                     /locus_tag="ACHUZQ_001401"
     CDS             21357..22802
                     /gene="tldD"
                     /locus_tag="ACHUZQ_001401"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1025135.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22930..23859)
                     /gene="aaeR"
                     /locus_tag="ACHUZQ_001402"
     CDS             complement(22930..23859)
                     /gene="aaeR"
                     /locus_tag="ACHUZQ_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1025136.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24042..24245
                     /gene="aaeX"
                     /locus_tag="ACHUZQ_001403"
     CDS             24042..24245
                     /gene="aaeX"
                     /locus_tag="ACHUZQ_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1025137.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24253..25185
                     /gene="aaeA"
                     /locus_tag="ACHUZQ_001404"
     CDS             24253..25185
                     /gene="aaeA"
                     /locus_tag="ACHUZQ_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1025138.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25191..27158
                     /gene="aaeB"
                     /locus_tag="ACHUZQ_001405"
     CDS             25191..27158
                     /gene="aaeB"
                     /locus_tag="ACHUZQ_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1025139.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27250..27522
                     /locus_tag="ACHUZQ_001406"
     CDS             27250..27522
                     /locus_tag="ACHUZQ_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1025140.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27578..27841)
                     /gene="yhcN"
                     /locus_tag="ACHUZQ_001407"
     CDS             complement(27578..27841)
                     /gene="yhcN"
                     /locus_tag="ACHUZQ_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1025141.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(28206..28676)
                     /gene="argR"
                     /locus_tag="ACHUZQ_001408"
     CDS             complement(28206..28676)
                     /gene="argR"
                     /locus_tag="ACHUZQ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1025142.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            29111..30049
                     /gene="mdh"
                     /locus_tag="ACHUZQ_001409"
     CDS             29111..30049
                     /gene="mdh"
                     /locus_tag="ACHUZQ_001409"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1025143.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         7300 a         7432 c         7935 g         7417 t
ORIGIN      
        1 gaattaaaac ctcgtaaatt gaaatatata ttcatgtggc gaatgtattt taggtaaata
       61 atatatatta tttatttgac gatttatagc aactcaatgt taccctctcg tataaataca
      121 cattagatga tagattaacc ttcgctattt tctcaccctg tgtcgaatat atttatttcc
      181 tgaataatta atcatggcaa aaagaaccaa agccgaagct ctgaagaccc ggcaagaact
      241 gattgaaact gccatcgccc agtttgcgca acatggcgta agcaagacga cgttcaacga
      301 cattgccgat gccgctaacg ttacgcgtgg cgctatctac tggcacttcg agaacaagac
      361 ccaactgttt aatgagatgt ggttgcaaca gccttcattg cgggagttaa tccaggaaca
      421 cttgacggct ggattagagc atgacccgtt tcaacaattg cgtgaaaaat tgattgtcgg
      481 cttgcaatat attgccaaaa ttccccgcca gcaggcgttg ctgaaaatct tatatcacaa
      541 atgtgaattt aatgatggga tgctggccga gggagtgata cgcgaaaaga tgggctttaa
      601 tccgcagact ctccgcgaag tattgcaggc gtgtcagcaa caaggttgtg tagcaaataa
      661 cctcgattta gatgttgtga tgattattat tgatggtgcc ttcagcggaa ttgttcaaaa
      721 ctggttaatg aatatggcgg gttatgatct ttataaacaa gcacccgcgc tggtcgataa
      781 cgtattaaga atgttcatgc cagatgaaaa cataacgaaa ttaattcatc aaacgaatga
      841 attaagtgtc atgtaaattc acattctgtc ctgaaaattc agttcgtcac agttaaccag
      901 ttttagtgga taaactgagt tcatattacg gcatttgtct gtttctgttg tcagcagttc
      961 aatccactgc atcagtaaag tatcaaacag aaaaacgaag acagcgaaaa cgacgagcca
     1021 ccaatacttg cgaatcatta cctgagcctg tgcatgttta accaagtctg gcaggaaata
     1081 tacacgaaat gaataatctg aaaaccaggc ttacacttcc tttactttgc aatgagatcg
     1141 gggtcaactt cgcttaaccg gcttcgtttt gacaggttgc cggtctttct ttttttcact
     1201 ttcgccagtt cttccgcgga gtaatgcgac gcgacatcca gccgtcgaag ccccattttg
     1261 atgtactcgc tgttgatctc aataccaatg aattttcgtc cgctggcgat ggctacggca
     1321 ccggtagtaa agctaccggc aaacgggtcg agaacgatat cgcctgggtt ggaagaggcg
     1381 agaataatgc gtttcagtaa ggcttccggt ttttgcgtcg ggtggttctc atattcatcc
     1441 atcaaataac gcacgcgcgg aaaatcccag acgttacccg gtactttttg atgattgtat
     1501 ggctgtggag gatttttgcg ataatcgatc aacgcgcgct gcgatccggt tttggcttct
     1561 accagaatag catcaccgtt gaatgtgtag ttctttgcgt ctttcaccat catcaggatg
     1621 ggttcgtaca tggagccgta gtgttttttc gcctgtactc cagaactgtc atatgaccag
     1681 acgatgcgac ttttgatggt aaaaagcttg cggcactgga gatcgataaa gggcatgttt
     1741 tccgtactgt tcataatgta catgctgccc tgctttttca gaacgcggtg gcactctaca
     1801 atcacttcaa acagccagtc gataaacaga tcttctttcc aggcttcgat cagaccatca
     1861 aaatttttac cgatgttata cggtgggtcg gcaaagatca gatcgacgct ttcagtaggt
     1921 agctttttaa gttcggcaag ggcatcaccg tgaataatgg tcttagcttc attaccaaac
     1981 cgggtcggtt cacatcctgt tctcatggtc actccctttg tgacacctat aaaaaaggcg
     2041 cttccccatg ccgagtagcg cctttttaat caagcattta gctaacctga attagttcat
     2101 gccgtatttt ttcaattttt tacgcagcgt accacggttg atgcccatca tcagcgcagc
     2161 acgggtctgg ttaccacggg tgtattgcat caccatgtcc aacaggggct gttctacttc
     2221 agccagtacc agctcataga ggtcattcac atcctgacca ttcagttgag caaaatagtt
     2281 cttcagtgcc tgtttaaccg agtcacgcag gggtttttgg gttacctgat cctgagagtt
     2341 aacggtagaa acggtcagta cgtcagaatt tacgcgttgt tcgaacatag ttctgtcagc
     2401 tctttatttc tgtttacgca aaattttcga agtatgcctc caacgcctcc agctgttcgc
     2461 tggcatcctc aatggcgttg aatgtgcgcc gaaactggtc atttggagcg tgttcctgga
     2521 gataccagga aacgtgttta cgtgcaattc ggtacccttt tgccggacca taaaagtcat
     2581 gcagttcccg aacgtgcgcg caaagcaagc gcttaacctc tgccaaaggc agcgggggca
     2641 gcaactcccc agtgtccaga taatgctgga tttcccgaaa gatccagggt cttccctgag
     2701 ctgcgcggcc tatcatcagg gcatccgccc ctgtatagtc gagcacagct ctggctttaa
     2761 gcgggtcagt aatgtcgcca ttcgcgataa ccggaatgga aactttctgc ttaactgccc
     2821 gaatactgtc gtactcagct tctccattga acaaacaggc gcgtgtacgg ccatgaatgg
     2881 tcagagcctg aatgccacag tcttcagcca gttgggcaat ctcttcgcag ttacggtgtt
     2941 ccggtgccca gccggtgcga atcttcaggg taacaggaac gtccactgca ttgacgacct
     3001 cggtaaggat cgatttaacg acatccgggt actgcaagag ggctgaacct gcgagcttgc
     3061 gattcacttt tttagccggg caacccatat tgatatcaat aatctgggca ccgctttcca
     3121 cgttaatacg tgctgcatct gccatttctt tcggatcgct accagcaatt tgcacggtgc
     3181 gaataccggg ttcatcaatg tgcaccatcc gtaaacgaga tttgtcgctt tcccaaacct
     3241 gtgggttaga agacatcatc tcggatactg tcaatccggc tcccatctcg tagcacaacg
     3301 tccgaaaagg tctgtctgta atgccagcca tgggcgctgc gatcaggcga tttctgagct
     3361 gatattgtcc gatgcgcatg agttaagaaa tgaccatact gtgactgcaa ggcggcgtat
     3421 attacgcatt ttttgcacga gatgaaaggc caaactttga ccaatcctct gagatggatc
     3481 aaagaattgc atttaaaatg agcgtggtga gataattact cataaaaatc atcatattag
     3541 aaaatagtga ctaaaattta cactcaaaga aatttgagta agttctcaat ttttctttat
     3601 gaatgaaaat tttggcacgc aaatttgcgt aaataaccgg caaatttacg tgccttttgt
     3661 gagcttgctc gcacttcgcc ccgcgtcacc ctacggcgat gcgaaggtta attcttacga
     3721 ccggtaatgc ggcaccactc ttctttttcc acgaccgggt ccagtgcgaa gctgtcgaca
     3781 taagcttcac aaacgctctc tgcctggctt gccagaatac cggaaaggcc cagcaaaccg
     3841 cctgaaaccg gcaggacgct gattaacggt gccagttcac gtaatgggcc tgcaaggatg
     3901 ttagcgacca ccacgtcggc tttcatttct tctggctgat ctttcggtaa gtagagttcc
     3961 agacggtcag aaacgccatt acgttcggcg ttgtcgcggc tagcctgaat cgcctgcgga
     4021 tcgatatcaa taccaatggc ttttgctgca cccagtttca gcgccgcgat agccagaatg
     4081 ccggaaccac agccaaagtc gatgactatt ttaccggtta aatcgaggct gtcgagccac
     4141 tgcaggcaca gagaggtagt tggatgggta cccgtaccaa acgccagccc tggatctaac
     4201 atcacgttga cggcgttttc gtccggcaca tcacgccagc tagggcagat ccacagtcgt
     4261 tcaccaaagc gcatcgggtg gaaattatcc atccattcgc gctcccagtc tttatcttcc
     4321 agttgttcga ttttatgcgc gaagcctgcg ccgagcagcg gatggttttc cagaatcgcc
     4381 accacgtcgt tcatatcggt ttcagcgtcg aacagaccaa tcacatcggt gtcgccccac
     4441 aggcgcgttt cgcccggcag cggctcaaat actggcgtat cgtgggtatc ctgaaaagtg
     4501 atagaaacgg cacccgcttc catcagcgca tcgctaagat cttcagcgtt cgcgccggtg
     4561 gtgttcagtt tcagttggat ccaaggcatg gcaaaactct ttatttatca gtagtcaaaa
     4621 cggtagcttg cgggacggat gtaccgaaac ggtttccgac caggaaagcc agcaaactta
     4681 gtagtaacga gggcacgata gggtggaagc ccaggtactg aatattcagc gtcgcgagta
     4741 cggcatacag cacgccgcca acgatcatcg cacttagcgc gcctttggcg ttggcgcgtt
     4801 cccagtaaag acccagcacc agcggccaca ggaaaacggc ttccagccca ccgaaggcca
     4861 gcaaattcag ccagatgatc atttctggcg gcttccaggc ggcaagcagc agcaacgcgc
     4921 cgagaactaa cgtaattacc gccgacatcc gcttcagacg cgtctcgttt tgcatttgat
     4981 ccggacggat attcagatag agatctttaa tgatcgtagc ggaactttgt agcaattggg
     5041 cgttaattgt cgacatgatc gcagccatag gtgcagccag aaagatcccg gcagcaaacg
     5101 gtggcagcac ttttaccatt aacgtcggga tcaccagatc cggtacggtg agatcgggga
     5161 tcactgcacg acctaacgct ccggctaggt gcataccgaa catcaggatt gcgactacaa
     5221 tcgtaccgat gatgatcccc cggtgtaccg ctttgctgtc tttataagag atacagcgca
     5281 ccgcagtatg cggcaggcca atcacgccaa agcacaccag tacccagaac gacgtcataa
     5341 aggcaggcga cagaatatcg tcagcgcctt gtggcgtaac cagttgcgga tcgatggttt
     5401 gcaaggtctg tactgcgttg cttaagccgc cagcggcatg taccacgcca ataagcagca
     5461 caacggtgcc aatcagcatc acaagccctt gcatggtgtc gttcagcacg ctggcgcgaa
     5521 agccgccaaa ggcggtatac aacgcaatgc tgataccgaa aatcagcagc ccggtttcat
     5581 aaggaatacc cgccgcggtt tccagcaggc gcgcaccgcc gataaactgc acggtcattg
     5641 ccccaacgaa cgcaaccagc aaactcaaac tcgccagcca caccagaaga cgactctggt
     5701 agcgggcaaa cagcatgtcg ttcagcgtca ctgcattgta gcggcgcgca agaatcgcaa
     5761 acttcttgcc gagaataccg agtgaaagcc agactgcagg aagctgaatc atcgccagca
     5821 atacccagcc cagcccgtat ttataagcag ctcctggccc gccgataaac gaactggcac
     5881 tgatataggt cgcggtgagc gtcatcgcca gcacaatacc gcccatagag cggctgccga
     5941 ggaaatactc attaaggaag gtgccggtgc tccgtttacg catcgcataa accgagatac
     6001 cgaacaccac caccagatag gcgaccagcg gtagaattac ttcaagctgc atcgtcatcc
     6061 tccagtggga tatcgcgata gataaatttc accatcgccc agcacagtcc aataaacagt
     6121 agcggcgtca ggatgcaggc catctcaaac cagcgcggaa agccggtaaa accgggggca
     6181 acgccaggta agtaagcggc tactaaccaa actgccagat acaaaagggt cagccccagc
     6241 gcccagcgcg cctctttatg ggcctgaaca aaacgagtgt ccattttttg tccctgatgg
     6301 gtgaagaaag cgaggattgt accttatggg ggttgtcgat ccccagtaat aaaaaaggcc
     6361 ggaaaatccg gccttttgac gcttttgcag tcttattttt cctgaagacc gagttttttc
     6421 tccagatagt ggatgttagt gccaccatgc tggaagttct cgtcattcat gatgcggatc
     6481 tgcagatcaa cgttggtttt gataccgtcg atgatcagct cctgcagcgc attcttcatg
     6541 cgggcaatcg ccacgtcacg gttttcaccg tagcaaatca gcttaccgat cattgagtca
     6601 tagtacggcg gtacggtgta gcccgcgtag atatgagact cccaacgtac gccaaaaccg
     6661 ccaggtgcgt ggaaacgggt gattttgcct gggcttggca ggaaggtgtt cggatcttcg
     6721 gcgttgatac gacattccac cgcatggccg cgaacgtgaa cttcttcttg cttgatcgac
     6781 agcggttgac cggcagcgat acggagctgt tctttgatca ggtcaacgcc agtgatcatt
     6841 tcggtaaccg ggtgttctac ctgaatacgg gtgttcattt cgatgaaata gaactcgccg
     6901 ttttcgaaca gaaactcgaa agtacctgca ccgcgatagc cgatatcaac acacgcttta
     6961 gcgcaacgtt cgccgatgta gcgacgcagt tccggggtaa tgcccggtgc tggcgcttct
     7021 tcgaccactt tctggtggcg gcgctgcatg gagcagtcac gttccgccag atagatagcg
     7081 ttgccctgac cgtcagccag tacctgaatc tcgacgtggc gaggattttc caggtatttc
     7141 tccatgtaaa ccatatcgtt gctgaaagca gctttcgctt ccgcacgggt catggagatg
     7201 gattgtgcca gttcagcgtc gccgcgcact acgcgcatac cgcgaccacc gccgccgccg
     7261 gaggctttga taatcaccgg ataaccaatg cgtttagcaa tggcacggtt tttatccata
     7321 tcgtcgccca acgggccgtc agaacccggt acgcaaggga cgcccgcttt tttcatcgcc
     7381 gcgattgcgg atactttgtc gcccatcagg cgaatggttt ctgctttcgg gccgatgaag
     7441 ataaagccgg agcgttcaac ctgctcggca aagttggcgt tctcggagag gaagccgtaa
     7501 cccggatgga ttgctactgc gccggtgatt tcagcggcgc tgatgattgc cgggatgttc
     7561 agataacttt ttactgacgg agcagggcca atacagaccg tttcatctgc cagtaatacg
     7621 tgttttagat cgcgatccgc gctggagtgc acagcgacag tcttgatgcc cagttcttta
     7681 caggcacgaa gaatacgcaa tgcaatctcg ccgcggttgg caataacaat tttatccagc
     7741 atgttcgcct cgttactcga tgacgaccag cggctcgtca aattctaccg gttgtccact
     7801 ttcgaccaga attgctttca cggtaccgga tttgtccgct tcgatctggt tcatcatttt
     7861 catggcttca acgatgcaca gggtatcgcc cacgttgact ttctgaccca cttcgatgaa
     7921 cgcttttgcg tccgggcttg gggtgcggta gaaagtacca accatcgggg aacgtacgat
     7981 gtgaccactg atttccgctg ctgctggcgc ttccatggaa ggaacggtcg ccggagcggc
     8041 tgcgttagat tgagctggct gctgcatcat tggtgcagcg taagcttgtt gcatcacagg
     8101 gaaacttgcg gcaggagctg cacggctaat gcgtactgac tcttcgcctt cagaaatttc
     8161 cagttcggag atgcctgatt cttcaaccag ctcgatcagt tttttaatct tacgaatatc
     8221 catgagtggg ttccgtactc tttgtttagt gtgattgtga caggcgtttc accgccgtct
     8281 gtaaagcgta tgccccgaca gctgtatgca tagcgataaa ttccagcagg ccgggacgcc
     8341 ggtctatttt gccccagagt atcaatgtta gacttgacgg acattgtgca gcgatctgct
     8401 gctatcctcc ggcaaaaaac aaaatatacc ttcgttgcgc caatgtcacc ttttccgcag
     8461 cgcaaaaact gcgtcaggga ggacgaggtc gcacattata acgatttcgt agcaattggc
     8521 agctaaatac tggtctcatc agggaagata atcaacagct aacatgtaaa taaccttcaa
     8581 caccgtgtaa tttgcaacaa gccgcacaat tcacgaaatt agcgccatca tcgacggaac
     8641 ttcttataac gtaaggtgaa aagctgtaaa gccagccctg cgaagatgag gggctgcaat
     8701 gagataatct tcacagacca caaataatgt agggatgcca ggatcgcgac aagatagacg
     8761 acattacgca gcggttgctg gggattaccc aattttcact gcatcgtctg gatagatgtg
     8821 aatgctaaag cacgcaaaat caaccgactg acaattctca gcattaaata ggttcgagtg
     8881 gttaatttgt agtcaaataa aatcagatta ttcaccccat ggtaccagaa atcagtaaca
     8941 ggttaagcgt agtgtcgccc aggcaaagca ccataaacct aacaggcgac ggaggcgtat
     9001 caataatggc tgtttagagg agtatcaatg cgcatcaatt tgtggtcagt cgaaatcatg
     9061 actttccagt ttggatgatg ttgtacaact tcctgaccaa ggtgttcgac gaaagctgtc
     9121 cggctaaccg cttttcccgt taaccgattg aataatgatg tcaaaatgag ttccctctct
     9181 tattattcct tgctaaatgg ttagttaacc ttcaccagcg tgcgaccctg gatctggtta
     9241 ttaatgatgg cctcggcaaa gttcggtgcc tctgacagag atatctcttt tgccgcctgg
     9301 gtatagaatg attccggtaa atcggcgacc agtcgctgcc aggcttgtgc gcggcgttct
     9361 ggtggcgtca ttactgaatc caccccttgc aaacggacat tacgcagaat aaatggcatg
     9421 accgtggttg gcagagtaaa accacccgcc agaccacagg ccgccacgca gccgccgtaa
     9481 ttcatttgcg ccagcacttt tgccagcact ttgtcgccaa cagtgtcaat tgccccagcc
     9541 cagacttgtt tttccagagg acgggattcg gcaaactcat cacgagggag aatacggctg
     9601 gcacctaaac ttttcagata ttcatgggta ctttcgcgac cggaaacggc aacgacctga
     9661 taacccaact tatgcagcag cgccacggcg gtactgccga cgccaccact ggcacccgtc
     9721 acgacaatct ccccgtcctg cgggcgaaca ccggcatctt ccagcgccat cacacacagc
     9781 atggcggtaa aaccggcagt accgataatc attgctttac gcgcgtccag cccttgcggc
     9841 atggcaacca gccagtcacc tttcactcgc gcctgctccg ccagcccacc ccagtggttt
     9901 tcaccaacgc cccagccagt gagtaacacc tcctgaccgg catgaaaacg cggatcttcg
     9961 ctggtgcgta cagttccggc aaaatcgatc ccaggaatca tcggaaaatt acggatgatt
    10021 tttcccttac cggtaatcgc cagcgcatct ttatagttca ggctcgacca gtgaacatcg
    10081 accgtgacat cgccctccgg caggcgactt tcgtccagag tctgtactga tgcgagagtt
    10141 ttgccatcct gctgttctaa aagtaacgcc tgcataagtg gtcctcatgt gcatgatgga
    10201 ttggaaaata atttctgaag attatactcg ctaatggaaa agcaaagccg atgaagcgca
    10261 ataaattgcg agataaatct gatttgctag tatgcccgct tcctcactat cggagttaac
    10321 acaaggatga gattaacgac gaaattttcg gcctttgtta cgctgctcac cgggttaaca
    10381 atttttgtga ctttgctggg ctgttcgcta agtttctaca acgcaattca gtataagttt
    10441 agtcatcgcg ttcaggcggt ggcgacggcg attgataccc accttgtgtc gaatgacttc
    10501 agcacattaa ggccacaaat taccgaatta atgatgtcgg cagatatcgt tcgtgtagac
    10561 ctgctccatg gtgataaaca ggtttatacc ctggccagaa atggtagtta tcgtccggtt
    10621 ggctccagcg atctgtttcg cgaactgagt gttccgttga taaagcatcc ggggatgtcg
    10681 ctgcgtctgg tttatcagga tccgatgggc aactatttcc attctttaat gactaccgcg
    10741 ccgctcacgg gggcgattgg ctttatcatt gttatgctct tcctggcggt acgctggtta
    10801 caacggcaac ttgccgggca agaattgctg gaaacccggg ctactcgtat cttaaacggt
    10861 gagcgtggct ctaatgtgtt gggaaccatc tatgaatggc cgcccagaac cagcagtgcg
    10921 ctagatacgc tgcttcgtga aattcagaac gcacgcgaac aacacagccg tcttgatacg
    10981 ctgatccgct cttatgccgc ccaggacgtg aaaaccggcc tcaataaccg actctttttc
    11041 gataatcagt tagcaacgtt actggaagat caggagaaag taggtaccca cgggatcgtg
    11101 atgatgattc gtctgccgga tttcaatatg ttgagcgata cttgggggca cagccaggtt
    11161 gaagaacagt tcttcactct gactaatctg ctgtcgacat ttatgatgcg ctaccctggc
    11221 gcactgctgg cgcgttacca ccgcagtgat tttgctgcgc tgttaccgca ccggacgtta
    11281 aaagaggcag agagcatcgc cggtcagtta atcaaagccg tcgatacctt gccgaacaat
    11341 aaaatgctcg atcgcgacga tatgatccac attggtatct gcgcctggcg tagtggtcag
    11401 gataccgagc aggtaatgga acatgcagag tctgccacgc gtaatgcggg attgcagggc
    11461 ggcaatagct gggctattta cgatgactcg ttgcctgaaa aaggacgcgg taatgttcgc
    11521 tggcgtacgc ttatcgagca aatgctcagt cgcggcggcc cgcgccttta tcaaaaaccg
    11581 gcggttactc gcgaaggtca ggttcatcat cgcgaactca tgtgccgcat cttcgatggt
    11641 aatgaagagg ttagctcggc ggagtatatg ccgatggtct tgcagtttgg cttatcggaa
    11701 gagtatgacc gtctgcaaat cagccgtctt attccactat tgcgttactg gccagaggaa
    11761 aatctggcga ttcaggttac cgttgagtcg ctgattcgcc cgcgttttca gcgttggctg
    11821 cgcgatacgt taatgcaatg tgaaaaatca caacgaaaac gcataattat tgaacttgca
    11881 gaggccgatg taggtcaaca tatcagtcgt ttacaacctg ttattcgttt agtgaatgct
    11941 ttaggggtac gggtagccgt caaccaggct ggtttgacgc tggtaagcac cagttggatc
    12001 aaagaactta atgttgagtt actcaagctc catccggggc tggtcagaaa cattgagaag
    12061 cgaacggaga accagctgct ggttcaaagc ctggtggaag cctgctccgg gaccagcacc
    12121 cacgtttacg ccaccggcgt gcgttcgcga agcgagtggc agaccctgat tcagcgcggt
    12181 gttacaggcg ggcaagggga ttttttcgcg tcctcacagc cacttgatac taacgtgaaa
    12241 aaatattcac aaagatactc ggtttaacct gccgtttaat ccgttttcac gtagaataac
    12301 gcgcgctgcg tctcatggga gtgtgcttgt ctgctcgcca gattgttgca gcacatatgc
    12361 agatgaatga ccttacgcgg ttgcaaacag gcgaggaatg ctgctgatgc attaagcctt
    12421 tctggactca ggcagagatt tgtaacaaag gaaacgaact gcactaattt tcaccgtagc
    12481 agatgatttt tgcgccttgt cgctgctgcg tgtggttggt aaagtaagcg gattttcttt
    12541 tccgccccag ctttcaggat tatcccttag tatgttgaaa aaatttcgtg gcatgttttc
    12601 caatgacttg tccattgacc tgggtactgc gaataccctc atttatgtaa aaggacaagg
    12661 catcgtattg aatgagcctt ccgtggtggc cattcgtcag gatcgtgccg gttcaccgaa
    12721 aagcgtagct gcagtaggtc atgacgcgaa gcagatgctg ggccgtacgc cgggcaatat
    12781 tgctgccatt cgcccaatga aagacggcgt tatcgccgac ttcttcgtga ctgaaaaaat
    12841 gctccagcac ttcatcaaac aagtgcacag caacagcttt atgcgtccaa gcccgcgcgt
    12901 tctggtttgt gtaccggttg gcgcgaccca ggttgaacgc cgcgcaattc gtgaatccgc
    12961 gcagggcgct ggtgcccgtg aagtcttcct gattgaagaa ccgatggctg ccgcaattgg
    13021 tgctggcctg ccggtttctg aagcgaccgg ttctatggtg gttgatatcg gtggtggtac
    13081 cactgaagtt gctgttatct ccttgaacgg cgtggtttac tcctcttccg tgcgcattgg
    13141 tggtgatcgt ttcgacgaag ctatcatcaa ctatgtgcgt cgtaattacg gttctctgat
    13201 cggtgaagcc accgcagaac gtatcaagca cgaaatcggt tcggcttacc cgggcgatga
    13261 agtccgtgaa atcgaagttc gtggccgtaa cctggcagaa ggtgttccac gcggttttac
    13321 cctgaactcc aacgaaatcc tcgaagcact gcaggaaccg ctgaccggta ttgtgagcgc
    13381 ggtaatggtt gcactggaac agtgcccgcc ggaactggct tccgacatct ccgagcgcgg
    13441 catggtgctc accggtggtg gcgcactgct gcgtaacctt gaccgtttgt taatggaaga
    13501 aaccggcatt ccagtagttg ttgctgaaga cccgctgacc tgtgtggcgc gcggtggcgg
    13561 caaagcgctg gaaatgatcg acatgcacgg cggcgacctg ttcagcgaag agtaatcgga
    13621 tgcaggcagg ggaaatgtct gtttaccctg cctggtctga tacgagaata cgcataactt
    13681 atgaagccaa tttttagccg tggcccgtcg ctacagattc gccttattct ggcggtgctg
    13741 gtggcgctcg gcattattat tgccgacagc cgcctgggga cgttcagtca aatccgtact
    13801 tatatggata ccgccgtcag tcctttctac tttgtttcca atgctcctcg tgaattgctg
    13861 gatggcgtat cgcagacgct ggcctcgcgt gaccaactag aacttgaaaa ccgggcgtta
    13921 cgtcaggaac tgttgctgaa aaacagtgaa ctgctgatgc ttggacaata caaacaggag
    13981 aacgcgcgtc tgcgcgagct gctgggttcc ccgctgcgtc aggatgagca gaaaatggtg
    14041 actcaggtta tctccacggt taacgatcct tatagcgatc aagttgttat cgataaaggt
    14101 agcgttaatg gcgtttatga aggccagccg gtcatcagcg acaaaggtgt tgttggtcag
    14161 gtggtggccg tcgctaaact gaccagtcgc gtgctgctga tttgtgatgc gacccacgcg
    14221 ctgccaatcc aggtgctgcg caacgatatc cgcgtaattg cagccggtaa cggttgtacg
    14281 gatgatttgc agcttgagca tctgccagcg aatacggata ttcgtgttgg tgatgtgctg
    14341 gtgacctccg gtctgggcgg tcgtttcccg gaaggctatc cggtcgcggt tgtctcttcc
    14401 gtaaaactcg atacccagcg cgcttatact gtgattcagg cgcgtccgac tgcaggactg
    14461 caacgtttgc gttatctgct gctgctgtgg ggcgcggatc gtaacggcgc taacccgatg
    14521 acgccggaag aggtgcatcg tgttgctaat gaacgtctga tgcagatgat gccgcaggta
    14581 ttgccttcgc cagacgcgat ggggccaaag ttacctgaac cggcaacggg gatcgctcag
    14641 ccgactccgc agcaaccgac gacaggaaat gcagctactg cgcctgctgc gcccacacag
    14701 cctgctgcta atcgctctcc acaaagggct acgccgccgc aaagtggtgc tcaaccgcct
    14761 gcgcgtgcgc cgggagggca atagtggcga gctatcgtag ccagggacgt tgggtaatct
    14821 ggctctcttt cctcattgcg ctgttgctgc aaatcatgcc ctggccggat aacctgattg
    14881 ttttccggcc aaactgggtg ttactcatct tgttgtattg gatcctggcc ttgcctcatc
    14941 gcgtaaatgt gggcacaggt tttgtgatgg gtgccatact ggatctgatc agcggctcga
    15001 cgcttggcgt acgcgtattg gcgatgagca tcattgctta cctggtggcg ctgaaatacc
    15061 agcttttccg caacctcgca ttatggcagc aggcgctggt cgtcatgttg ctttcgctgg
    15121 tggtggatat tattgttttc tgggcagagt ttttagtgat taacgtctct ttcagaccgg
    15181 aagtgttctg gagtagtgta gtcaatgggg tgctctggcc gtggattttc ttgctgatgc
    15241 gtaaagtccg tcagcagttt gcagtgcaat aaaggtttct atgacttctc tgtatttagc
    15301 ttccggttct ccgcgtcgtc aggagttact tgcgcaactt ggtgtgacct ttgaacgtat
    15361 tgttacgggc attgaggagc agcgtcagcc gcaggagagc gcgcagcagt atgttgtgcg
    15421 tctggcgcgc gagaaagcac aggcgggtgt cgcgcaaacg gcgcaggatc tcccggtgct
    15481 gggtgcggat actatcgtta tcctgaatgg agaagtgctg gagaaaccgc gcgacgcaga
    15541 gcatgcggcg cagatgttgc gcaaattatc gggtcagacc catcaggtga tgacggcagt
    15601 ggcgttggcc gacagccagc acattctcga ttgcctggtg gtcaccgatg tgactttcag
    15661 aacgttaaca gacgaagaca tcgcgggcta tgtcgccagc ggtgaaccgt tagataaagc
    15721 aggtgcatac ggtattcagg ggctgggtgg ctgttttgtc aggaagataa atggcagcta
    15781 tcacgccgta gtcggcttac ctctggttga aacgtatgaa ttattaagta attttaacgc
    15841 actgcgtgag aaaagggata aacatgacgg ctgaattgtt agtaaacgta acgccttcgg
    15901 aaacgcgagt ggcgtatatt gatggcggta ttctgcagga aattcatatt gaacgtgagg
    15961 cgcgacgcgg aatagtaggc aatatctaca agggtcgtgt aagtcgtgta cttccgggta
    16021 tgcaggcggc ttttgtagat attgggctgg ataaagccgc gtttcttcat gcatccgata
    16081 tcatgccgca caccgaatgt gtggcgggtg aagaacaaaa gcaattcacg gtgcgcgaca
    16141 tctcggaact ggttcgtcag gggcaagatc tgatggtgca ggtggtgaaa gatccgcttg
    16201 gcactaaagg tgcgcgcctg accaccgata tcacgctgcc ttctcgctat ctggtcttta
    16261 tgccaggggc ttctcacgtt ggggtttccc aacgtattga aagcgaatca gaacgtgaac
    16321 gcctgaaaaa agtggtcgca gagtattgcg acgagcaggg cgggtttatc atccgtaccg
    16381 cagcggaagg ggttggcgag gctgaactgg cctccgatgc cgcttatctg aaacgcgtct
    16441 ggaccaaagt tatggagcgt aaaaagcgcc cgcagacccg ttatcagctg tacggcgaac
    16501 tggcgctggc tcagcgtgtt ctgcgtgatt tcgccgacgc cgaactggac cgcattcgcg
    16561 ttgactcacg cctgacttac gaagcattac ttgagttcac ctcggagtac attcccgaga
    16621 tgaccagcaa gctggagcat tacaccggac gccagccgat tttcgatctc tttgatgtcg
    16681 aaaacgaaat ccagcgagcg ctggaacgca aagtagaact gaaatccggt ggttatctca
    16741 ttatcgacca gaccgaagcg atgaccaccg tggacatcaa taccggagcg tttgtcggtc
    16801 atcgcaatct ggacgacacc attttcaata ccaatattga agcgacgcag gctatcgctc
    16861 gccagttacg gttgcgtaat ctgggcggga ttatcattat tgatttcatc gatatgaata
    16921 atgaagatca ccgccgccgc gtgctgcact cgctggagca ggcgttgagc aaagaccggg
    16981 tgaaaaccag cgttaatggt ttttcggcgc tggggctggt ggagatgacg cgtaaacgca
    17041 cccgcgaaag cattgagcac gtactgtgta acgaatgccc aacctgccac ggtcgcggaa
    17101 cggtgaaaac cgtggaaacg gtatgctatg aaatcatgcg cgagattgtt cgtgtccacc
    17161 atgcttacga ctccgaccgt ttcctggtct atgcttctcc ggcagtagcc gaagccttga
    17221 aaggcgaaga gtcacactcg ctggcggaag tggaaatttt cgttggcaaa caggttaaag
    17281 tacaaattga accgctctat aaccaggagc agtttgacgt cgtaatgatg taaacagatg
    17341 ctgggccgcc atccggcaaa gggtttttga gtcacatttt tagcagacaa ggagtgacgg
    17401 gtgaggcgat tgccggggat tttactgctt actggagccg cgctcgttgt gatcgctgcc
    17461 ctgctggtta gcggcctgcg tattgcttta ccgcatcttg acgcctggcg tccggaaatc
    17521 ctcaacaaaa tagaatccgc gactggcatg ccggtagaag ccagtcagct ctcagccagc
    17581 tggcagaatt ttggcccgac gcttgaagca cacgacatcc gtgcagaact aaaagatggc
    17641 ggcgaatttt cggttaaacg cgttactctg gcgctggatg tctggcagag cctgttacat
    17701 atgcgctggc agtttcgcga cctcactttc tggcagctgc gctttcgcac caacactcct
    17761 atcaccagcg gtggtagcga tgacagtctg gaagccagtc acatcagcga tctgtttctt
    17821 cgtcaatttg accatttcga tctccgcgac agtgaagtca gtttcctgac gccatccggt
    17881 cagcgtgccg agctggcgat cccacaactc acctggctga acgatccacg tcgacaccgt
    17941 gcggaaggcc tggtaagcct ctccagcctt accggacagc acggcgtgat gcaggtgcgc
    18001 atggatttgc gcgatgatga ggggttgtta agcaatggtc gcgtctggct acaggcggat
    18061 gacatcgacc tgaagccgtg gctcggtaaa tggatgcagg acaatattgc tctggaaacg
    18121 gcacagttct cccttgaagg ctggatgacg atcgacaaag gcgatgtaac cggcggtgac
    18181 gtctggctga aacagggcgg tgccagctgg ttgggcgaga agcaaacgca tacgctgtcg
    18241 gtggataatc tgaccgcgca tattacgcgt gaaaatccgg gctggcagtt ctctattccc
    18301 gatacacgga tcacgatgga cggcaaaccc tggccgagcg gagcattgac gctggcctgg
    18361 ataccggaac aggacgttgg cggcaaagac aataaacgca gtgacgaact ccggattcgc
    18421 gccagtaatc tggagctggc aggcctggag ggcatacgcc cgctggccgc gaaactttca
    18481 cctgcactgg gtgatgtttg gcgctccaca caaccgagcg gcaagattaa cactctggcg
    18541 ctggatatcc cgcttcaggc ggcagacaag acccgttttc aggcatcgtg gagcgatctg
    18601 gcctggaagc aatggaaatt attaccgggt gcggaacact tctccgggac gctttccggc
    18661 agcgttgaaa atggtttgct taccgcgtcg atgaagcagg caaagatgcc ttacgaaacg
    18721 gtattccgtg cgccactgga aatcgccgac ggccaggcaa ctataagctg gctgaacaat
    18781 gacaaaggtt tccagctgga tgggcgtaat attgacgtta aagccaaagc cgtccatgcg
    18841 cgcggcggtt ttcgttacct gcaacctgct aacgatgaac cctggctggg tattctggct
    18901 ggcatcagta ccgatgatgg ttcacaagcc tggcactatt tcccggaaaa cttgatgggt
    18961 aaagacctgg ttgattactt aagtggcgcg attcagggcg gtgaagcgga taacgcgacg
    19021 ctggtttatg gtggcaatcc gcaactcttc ccctataaac acaacgaagg tcagtttgaa
    19081 gtgctggtgc cgctgcgcaa cgcgaagttt gccttccagc cggactggcc tgcattaact
    19141 aaccttgata ttgaactgga ctttattaac gacggtttat ggatgaaaac cgatggcgtt
    19201 aatctgggcg gcgtgcgcgc gagtaatctc accgcagtga tccctgacta ctcgaaagaa
    19261 aaactgctga ttgacgctga cattaaaggt ccgggtaaag ccgttggccc ttactttgat
    19321 gagacaccgc tgaaagattc tctgggtgcg accctgcaag aactccagct cgacggcgat
    19381 gtgaatgctc gcttacatct tgatatcccg ctgaacggcg agctggtaac cgcgaaaggt
    19441 gaagtgacgc tgcgtaataa cagtctgttt atcaaaccac tcgacagcac cctgaaaaat
    19501 ttgagcggta aattcagctt tatcaatggc gatctgcaaa gtgaaccact gacagcaagc
    19561 tggtttaatc agccgttgaa cgtggatttt tccaccaaag aaggggcaaa agcctaccag
    19621 gttgcggtga atctcaacgg taactggcaa ccggcgaaaa ccggcgttct gcctgaagcg
    19681 gtgaacgaag cattgagtgg cagcgtggcg tgggatggta aagtgggcat tgatctgcct
    19741 tatcatgctg gcgcgaccta taacgtagag ctgaacggcg atctgaataa tgtgagtagt
    19801 cacttacctt caccgttagc caaacctgcg ggtgaaccac tagcggtaaa cgttaaggtt
    19861 gatggcaatc tcaacagctt tgaattaacc ggacaggctg gtgcggataa ccatttcaat
    19921 agccgctggt tgcttggtca aaagctgacg ctcgatcgtg ctatttgggc ggcagacagt
    19981 aaaacgctcc cgccgttgcc ggaacaaagt ggcgttgaac tcaatatgcc gccgatgaat
    20041 ggtgccgagt ggctggccct gttccagaaa ggcgctgcgg agagtgtcgg tggtgcagcg
    20101 agtttcccac aatacataac gttacgtacg cctatgttgt cactgggaaa tcagcaatgg
    20161 aataacctga gtattgtttc gcaaccgacg gcaaatggca ccctggttga ggcgcaaggg
    20221 cgtgaaatca acgccacgct agcgatgcgt aataacgcgc cgtggctggc gaatatcaaa
    20281 tatctttatt acaacccgag cgtggcgaaa actcgtggtg attcaacgcc gtcatcacct
    20341 ttcccgacaa cggagcgcat taacttccgt ggctggccgg acgcacaaat acgatgcaca
    20401 gagtgctggt tctgggggca aaaattcggt cgcattgaca gtgatatcac catttctggc
    20461 gatacgttaa cgctgaccaa tggactgatt gatactggtt tctcgcggct tactgccgat
    20521 ggtgaatggg ttaataatcc ggggaatgaa cgtacctcgc tgaaaggaaa actgcgcggg
    20581 cagaaaattg atgccgccgc agaatttttt ggtgtcacga cgcccatacg ccagtcgtca
    20641 tttaatgtgg attacgattt acactggcgc aaagcaccct ggcagccaga tgaagcgacg
    20701 ttgaatggca tcattcatac tcaactgggt aaaggcgaaa ttaccgaaat caataccgga
    20761 catgccgggc aattgctgcg cttattgagc gtagatgccc tgatgcgtaa gctgcgtttt
    20821 gatttcagag acacttttgg cgaagggttc tattttgact ccattcgcag caccgcgtgg
    20881 attaaagacg gggttatgca caccgacgac acgctggtgg atggcctgga ggcggatatc
    20941 gccatgaaag ggtcggtaaa tctggtacgt cgcgacctga atatggaagc ggttgtcgca
    21001 ccagagattt ctgcgacggt gggcgtggct gcggcttttg cggttaaccc cattgttggc
    21061 gcggcagttt ttgccgctag taaagtgctg gggccgctgt ggagcaaagt ctccattttg
    21121 cgctatcaca tttcgggtcc gctggacgat ccgcaaatca acgaagtgtt gcgccaaccg
    21181 cgtaaagaaa aagcgcaatg atttgacgag ggcgcgtaat tgccccaatc tcatatgata
    21241 atcgttgcca aaggccaacg agccagaaca taaccgtagg ccggataggg cgttcatgcc
    21301 gcatccggca gccgtaaaaa atcctctact gcagtaacta acgagtagca aaaacgatga
    21361 gtctgaacct ggtaagtgaa caattgctag cggcgaacag cctgaaacat caggacttgt
    21421 tcgcgatcct cggtcaactg gccgaacgtc gccttgatta tggcgatctc tattttcagt
    21481 cgagctatca cgaatcctgg gttttagaag accgcattat taaagatggt tcttacaaca
    21541 tcgatcaggg cgttggcgtg cgtgcaatca gcggtgaaaa aaccggattt gcttacgctg
    21601 accaaatcag cctgctggcg ctggaacaga gtgcgcaagc ggcgcgcacc atcgtccgtg
    21661 atagtggtga tggcaaagta cagacgctgg gcgcggtaga gcatagcccg ttgtatacct
    21721 cggtagatcc gctgcaaagc atgagccgtg aagagaagct ggatatcctg cgtcgcgtcg
    21781 ataaggttgc tcgcgaagcg gacaagcgcg tacaggaagt gactgccagc ctcagtggtg
    21841 tctatgaatt aattctggtt gcggccaccg acggcacgct agcggcggat gtccgtccgc
    21901 tggtgcgtct ttccgtgagc gttctggtcg aagaagatgg caaacgcgaa cgcggtgcca
    21961 gtggcggcgg cggtcgtttt ggttatgagt tcttccttgc cgatcttgac ggcgaagtcc
    22021 gtgcggatgc atgggcaaaa gaagcagtgc gtatggcgct ggtcaatctt tctgccgttg
    22081 ctgcaccagc gggcaccatg ccggtagtac ttggcgcagg ttggccgggc gtgctgttgc
    22141 atgaagcggt aggtcacggt ctggaaggcg acttcaaccg ccgcggcact tcagtattta
    22201 gtgggcaggt cggggagctg gtggcttcag aactgtgtac cgtggttgat gacggcacga
    22261 tggtcgatcg ccgtggttcg gtggcgattg atgacgaagg tacgccaggc cagtacaacg
    22321 tgctgattga gaacggcatt ctgaaaggct acatgcagga taaactcaac gcgcgtttga
    22381 tggggatgac gccgactggc aacggtcgcc gtgaatccta cgcccatctg cccatgccgc
    22441 gtatgactaa cacctatatg ctgccgggta aatcgacccc gcaggaaatt attgaatccg
    22501 ttgagtacgg tatctatgca ccgaactttg gtggcggtca ggtggatatc acctccggca
    22561 aattcgtttt ctccacttca gaagcatatc tgattgaaaa cggtaaagta acgaagccgg
    22621 tgaaaggcgc aacgttgatt ggttccggta tcgaaaccat gcagcagatt tcgatggttg
    22681 gcaacgacct gaaactggat aacggcgtgg gtgtctgcgg taaagaaggg caaagtttgc
    22741 cggttggcgt gggccagcca acgctgaaag ttgataacct gactgttggc ggtactgcgt
    22801 aataatttca ttattttcag aaggataatt aatctttcta cgtgcacgaa cggtcccctc
    22861 gctcctctgg ggttagggtg aggggaaccc gttggcacag gtttgtagaa cgtaacagta
    22921 caatatgaat tacttctctt tcccgcgccc gtgcatctcc tgaaacaatt taccgacctc
    22981 aacaaaataa tccgtcagcg agttgatcac gacctgtacc ttcagcggca gcttatcttt
    23041 ttcggtatat aacgcataaa ccgggcgtgg atctgactga taacgcggca gcaggatctc
    23101 cagctcaccg cgattgatct cgttgatcac ccacatcagc ggcacgtagg cgatcccggc
    23161 acccgccgtc agccagcgca ccagcgtcat cggatcatta gtcacaaatc ttccttgtgg
    23221 gatcaggcga gtcgagatcc cttccggtgc gatcagttca aattcattgt cgggccgcac
    23281 gctgtattca agccatgaat gactactcaa atcggcgggt ttttccggta tgccgtattg
    23341 tgtgagatag cttttcgcgg cgcacaccac catcggcatc gcgcccagac ggcgggaaaa
    23401 caggctggaa tcctgcaacg cgccgacgcg gatcaccaca tccagaccgt cggcaatcag
    23461 gtcgggggct ggaattccgg taaccagatt gacgctcaaa cctgggtatt ctttcagcat
    23521 ttttgctgtc agcccggcga gaacattttg tgccatagtt gaagaacagc caatgcgcag
    23581 cgtcccgatg ggggtgttat tgaaggcata cagttgctca tgaacatcct gcacttcatg
    23641 aagcatccga cggcagccct ggtagtaaat tctgccggct tcggtcaggc caatgctgcg
    23701 tgtgctacgg tttaacagct ttacctgcaa ctcatcttcc agttttgaca ccgtctgact
    23761 gatggacgaa acgctcatct gtagctgtct ggcggcggcg gtaaaagagc caaattcaac
    23821 tactttggca aacaccgaca tgcgttttag tcgttccatt attcactctg acttaaaagt
    23881 gatttagatc acataatata gataacagca taacagttac gctaatatat taaatatcaa
    23941 tctacagcaa tgttgctctc gcccggcttt ccatgccctt ctctgcggcg acagatgctg
    24001 aaaataataa cgcctgctct ctctttacaa ccaaggtcaa catgagtctg tttcccgtta
    24061 tcgtggtgtt tggactgtcc ttcccaccga tattttttga attgctttta tcactggcga
    24121 ttttctggct ggtgcgccgg gtacttgtgc caacaggtat ctacgacttt gtctggcatc
    24181 cggcgttgtt caacaccgcg ctctattgct gcttgtttta tttgatatcg cgactgttcg
    24241 tttgaggttg aagtgaaaac actaataaga aaattctccc gtacggccat cacggtcgta
    24301 ttagtcattc tagccttcat cgcaattttt aatgcctggg tctattacac cgaatccccc
    24361 tggacgcgtg acgcgcgctt tagcgctgac gtcgttgcga tcgcgccgga cgtttctgga
    24421 ctcattaccc aggtgaatgt tcatgataac cagctggtga aaaaaggaca gatactgttc
    24481 accatcgacc agccgcgcta tcaaaaggcg cttgaggaag cgcaagccga tgttgcttat
    24541 tatcaggtac tggcacagga gaaacgccag gaggccggac gtcgtaaccg tctcggtgtg
    24601 caggcgatgt ctcgcgaaga gatcgaccag gccaacaacg tactacaaac ggttctgcat
    24661 cagttagcga aagcgcaggc gacccgcgat ctggcaaaac tggatcttga acgcacggtg
    24721 atccgcgcgc cagcagatgg ctgggtgacc aacctcaacg tctataccgg tgagtttatt
    24781 actcgaggat caacggcggt tgcgctggtg aaacagaact ccttctatgt actggcctat
    24841 atggaagaaa ctaagctgga aggggggcgt ccggggtatc gtgcagagat cacgccgctt
    24901 ggcagtaaca aagtgctgaa agggactgtt gatagtgttg ccgcaggggt caccaacgcc
    24961 agcagcacgc gtgacgacaa agggatggcg actatagact ctaatcttga atgggtgcgt
    25021 cttgcgcaac gtgttccggt tcgtattcgt ctcgacaacc agcaagagaa catctggcct
    25081 gcgggcacca ctgctacagt ggtggtcact ggcaaacaag atcgcgacga aagccaggat
    25141 tcgttcttcc gtaaaatggc ccatcgcctg cgtgagtttg gttaatcacg atgggtattt
    25201 tctccattgc taaccaacat attcgctttg cggtaaaact ggcgaccgcc attgtactgg
    25261 cgctgtttgt tggctttcac ttccagctgg aaacgccacg ctgggcggta ctgacagcgg
    25321 cgattgttgc cgccggtccg gcctttgctg cgggaggtga accgtattct ggcgctattc
    25381 gctatcgtgg ctttttgcgc atcatcggca catttattgg ctgtattgcc ggactggtga
    25441 tcatcattgc gatgatccgc gcaccattat tgatgattct ggtgtgctgt atctgggccg
    25501 gtttttgtac ctggatatcc tcgctggtac gaatagaaaa ctcgtacgcg tgggggctgg
    25561 ccggttatac cgcgctgatc attgtgatca ccattcagcc ggaaccattg cttacgccgc
    25621 agtttgccgt cgaacgttgt agcgagatcg ttatcggtat tgtgtgtgcg attatggcgg
    25681 atttgctctt ttctccgcga tcgatcaaac aagaagtaga tcgagagctg gaaagtttgc
    25741 tggtcgcgca atatcaatta atgcaactct gtatcaagca tggcgatggt gaagttgtcg
    25801 ataaagcctg gggcgacctg gtgcgacgca ccacggcact acaaggcatg cgcagcaacc
    25861 tgaatatgga atcttcccgc tgggcgcggg ccaatcgacg tttaaaagcg atcaatacgc
    25921 tatcgctgac gctgattacc caatcctgcg aaacttatct tattcagaat acgcgcccgg
    25981 aattgatcac tgatactttc cgcgaatttt ttgacacgcc ggtagaaacc gcgcaggacg
    26041 tccacaagca gctcaaacgc ctgcggagag ttatcgcctg gaccggggaa cgggaaacgc
    26101 ctgtcaccat ttatagctgg gtcgcggcgg caacgcgtta tcagcttctc aagcgcggcg
    26161 ttatcagtaa cacaaaaatc aacgccaccg aagaagagat cctgcaaggc gaaccggaag
    26221 taaaagtaga gtcagccgaa cgtcatcatg caatggttaa cttctggcga accacacttt
    26281 cctgcattct gggcacgctt ttctggctgt ggacgggctg gacttccggc agtggtgcaa
    26341 tggtgatgat tgcggtcgtg acgtcactgg caatgcgttt gccgaatcca cgcatggtgg
    26401 cgatcgactt tatctatggg acgctggccg cgctgccgtt agggctgctc tactttttgg
    26461 tgattatccc taatacccaa cagagcatgt tgctgctgtg tattagcctg gcagtgctgg
    26521 gattcttcct cggtatagaa gtacagaaac ggcgactggg ctcgatgggg gcactggcca
    26581 gcaccataaa tattatcgtg ctggataacc cgatgacttt ccatttcagt cagtttctcg
    26641 acagcgcatt agggcaaatc gtcggctgtg tgctcgcgtt caccgttatt ttgctggtgc
    26701 gggataaatc gcgcgacagg actggacgtg tactgcttaa tcagtttgtt tttgccgctg
    26761 tttccgcgat gactaccaat gtggcacgtc gtaaagagaa ccacctcccg gcactttatc
    26821 agcagctgtt tttgctgatg aataagttcc caggggattt gccgaaattt cgcctggcgc
    26881 tgacgatgat tatcgcgcac cagcgcctgc gtgatgcgcc gatcccggtt aacgaggatt
    26941 tatcggcgtt tcaccgacaa atgcgccgca cagcagacca tgtgatatct gcccgtagcg
    27001 atgataaacg tcgtcggtac tttggccagt tgctggaaga actggaaatc taccaggaaa
    27061 agctacgcat ctggcaagcg ccaccgcagg tgacggaacc ggttcatcgg ctggcgggga
    27121 tgctccataa gtatcaacat gcgttgaccg atagttaagt caaaaccgac gccaaaagcg
    27181 tcggtttttt catggctata cttagcgatg cacggcagaa ctcgccgcga aacgtgacgg
    27241 tggcaacaga tgaatattta tacctttgat tttgatgaga ttgagagtca ggaggatttt
    27301 tatcgtgact ttagccaaac ctttggtctg gctaaagata aggtacgcga tctcgactca
    27361 ctatgggatg tgttaatgaa cgatgtcctg ccgctaccac ttgagattga atttgttcat
    27421 ctgggagaga aaacgcgtcg ccgttttggc gcgttaatat tgctgtttga tgaggcagag
    27481 gaagagctgg aagggcattt gcgttttaat gttcgtcatt agcgcaaaaa aaagcccccg
    27541 aaccgggggc aatatcgtcg gacaagacga tgagggttta tttgtacagt tcagccgtag
    27601 cgtgccaggt gtcaccgcta cgagcttcag taatctggta ggccgttgcg cctttctctt
    27661 ccgctttttt gttcagcatt tcacgcatat ccattggcga agacgccaca ccacttacgg
    27721 atacggtccc gattgcttca cgattttgtg cttgtgcagt atcaatggag tcggcagcga
    27781 atgcaccgaa agagagaaca gaaagtacgc ttaatgcagc tacagtggtt ttgattttca
    27841 tgatttttac ctcgacataa tcttttagct gggcctttgt ttcgtgaccc atatcacaaa
    27901 atcaagcata cactaattat tgaactaatt aataccacgc taactatttc agttgatact
    27961 aagtgttaaa aatgtgaatt ttaataacaa aaaagaatta aaaacacggt tatttattgt
    28021 taattctagt attttcaatt agataatgaa ttttacataa tgtgcttttt ttaccgtatg
    28081 tattcattgt gtgaatgata tgtcgcggta aaacgcacta ttcgttaaat taatcgcagg
    28141 ggaaagcagg gggttgagag gaataagcaa cattttcccc gccgccagaa gcgacggggc
    28201 agagattaaa gctcctggtc gaacagctct aaaatcgctt cgtacaggtc tttgacggtg
    28261 aaaccgttag caggggtggt aaagatggtg tcatcgccag cgatggtgcc cagaatacct
    28321 tctgctttgc ccagtgagtc cagcaggcga gcaattaact gcgccgcgcc agggctggta
    28381 tgaatcacga caactgcatc gttgtagtcg atatccagca ccagattctt caatggactg
    28441 gaggtggttg gtacacccag ttcagctggc aggcagtaaa ccatttccat tttggcattg
    28501 cgtgtacgta cagcaccaaa cttggtcaac atccgcgaga ctttagactg attaatattg
    28561 tcaaagcctt gctcctgcaa cgcggcgacg atttcgccct gggagctaaa tttctcttct
    28621 ttaagtaatg ctttaaatgc tttaactagt tcttcttgct tagccgagct tcgcataagt
    28681 cacccgatat ggtggttgat acaacattat tgtgcataca gatgaatttt tatgcaaaca
    28741 gtcagccctg aagaaggctg aaataatgtt atgaaagagc gggattttat caaatttcgt
    28801 tattgagaaa catgcctgcg tcacggcatg caaattctgt ttaaaagtaa attaattgtt
    28861 atcaaattga tgttgttttg gctgaacggt agggtatatt gtcaccacct gttggaatgt
    28921 tgcgctaatg cataagcgac tgttaattac gtaagttagg ttcctgatta cggcaattaa
    28981 atgcataaac gctaaacttg cgtgactaca cattcttgag atgtggtcat tgtaaacggc
    29041 aattttgtgg attaaggtcg cggcagcgga gcaacatatc ttagtttatc aatataataa
    29101 ggagtttagg atgaaagtcg cagtcctcgg cgctgctggc ggtattggcc aggcgcttgc
    29161 actactgtta aaaacccaac tgccttcagg ttcagaactc tctctgtatg atatcgctcc
    29221 agtgactccc ggtgtggctg tcgatctgag ccatatccct actgctgtga aaatcaaagg
    29281 tttttctggt gaagatgcga ctccggcgct ggaaggcgca gatgtcgttc ttatctctgc
    29341 aggtgtagcg cgtaaaccgg gtatggatcg ttccgacctg tttaacgtta acgccggcat
    29401 cgtgaaaaac ctggtacagc aagttgcgaa aacctgcccg aaagcgtgca ttggtattat
    29461 cactaacccg gttaacacca cagttgcgat tgctgctgaa gtgctgaaaa aagccggtgt
    29521 ttatgacaaa aacaaactgt tcggcgttac cacgctggac atcattcgtt ccaacacttt
    29581 tgttgcggaa ctgaaaggca aacagccagg cgaagttgaa gtgccggtta ttggcggtca
    29641 ctctggtgtt accattctgc cgctgctgtc acaggttcct ggcgttagtt ttaccgagca
    29701 ggaagtggct gatctgacca aacgcatcca gaacgcgggt actgaagtgg ttgaagcgaa
    29761 agccggtggc gggtctgcaa ccctgtctat gggccaggca gctgcacgtt ttggtctgtc
    29821 tctggttcgt gcactgcagg gcgaacaagg cgttgtcgaa tgtgcctacg ttgaaggcga
    29881 tggtcagtac gcccgtttct tctctcaacc gctgctgctg ggtaaaaacg gcgtggaaga
    29941 gcgtaaatct atcggtaccc tgagcgcatt tgaacagaac gcgctggaag gtatgctgga
    30001 tacactcaag aaagatatcg ccttgggcga agagttcgtt aataagtaat tgattagcga
    30061 ataataaaaa accggagcac agac
//
LOCUS       DAZPBM010000034        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPBM010000034 DAZPBM010000000
VERSION     DAZPBM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.034"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHUZQ_001410"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHUZQ_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR1025144.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHUZQ_001411"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHUZQ_001411"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1025145.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUZQ_001412"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUZQ_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR1025146.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHUZQ_001413"
     CDS             complement(3644..4030)
                     /locus_tag="ACHUZQ_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1025147.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUZQ_001414"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUZQ_001414"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR1025148.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUZQ_001415"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUZQ_001415"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1025149.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUZQ_001416"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUZQ_001416"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1025150.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUZQ_001417"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUZQ_001417"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1025151.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUZQ_001418"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUZQ_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1025152.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUZQ_001419"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUZQ_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1025153.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUZQ_001420"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUZQ_001420"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1025154.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHUZQ_001421"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHUZQ_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1025155.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUZQ_001422"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUZQ_001422"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1025156.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUZQ_001423"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUZQ_001423"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1025157.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUZQ_001424"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUZQ_001424"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1025158.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUZQ_001425"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUZQ_001425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1025159.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUZQ_001426"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUZQ_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1025160.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHUZQ_001427"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHUZQ_001427"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1025161.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUZQ_001428"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUZQ_001428"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1025162.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUZQ_001429"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUZQ_001429"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1025163.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUZQ_001430"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUZQ_001430"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1025164.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUZQ_001431"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUZQ_001431"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1025165.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUZQ_001432"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUZQ_001432"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1025166.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUZQ_001433"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUZQ_001433"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1025167.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHUZQ_001434"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHUZQ_001434"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1025168.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZPBM010000035        29126 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPBM010000035 DAZPBM010000000
VERSION     DAZPBM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29126
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.035"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUZQ_001435"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUZQ_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1025169.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHUZQ_001436"
     tRNA            1230..1305
                     /locus_tag="ACHUZQ_001436"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHUZQ_001437"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHUZQ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1025170.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHUZQ_001438"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHUZQ_001438"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1025171.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHUZQ_001439"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHUZQ_001439"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1025172.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHUZQ_001440"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHUZQ_001440"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1025173.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="ACHUZQ_001441"
     CDS             complement(4868..5305)
                     /locus_tag="ACHUZQ_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR1025174.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="ACHUZQ_001442"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="ACHUZQ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHUZQ_001443"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHUZQ_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR1025175.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="ACHUZQ_001444"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="ACHUZQ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR1025176.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8268..9080
                     /gene="pduB"
                     /locus_tag="ACHUZQ_001445"
                     /pseudo
     CDS             8268..9080
                     /gene="pduB"
                     /locus_tag="ACHUZQ_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="ACHUZQ_001446"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="ACHUZQ_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR1025177.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="ACHUZQ_001447"
     CDS             10773..11441
                     /locus_tag="ACHUZQ_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR1025178.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="ACHUZQ_001448"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="ACHUZQ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR1025179.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="ACHUZQ_001449"
     CDS             11984..13816
                     /locus_tag="ACHUZQ_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR1025180.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="ACHUZQ_001450"
     CDS             13806..14156
                     /locus_tag="ACHUZQ_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR1025181.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="ACHUZQ_001451"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="ACHUZQ_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR1025182.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="ACHUZQ_001452"
     CDS             14475..14897
                     /locus_tag="ACHUZQ_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR1025183.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="ACHUZQ_001453"
     CDS             14900..15532
                     /locus_tag="ACHUZQ_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR1025184.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="ACHUZQ_001454"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="ACHUZQ_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR1025185.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="ACHUZQ_001455"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="ACHUZQ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR1025186.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="ACHUZQ_001456"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="ACHUZQ_001456"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR1025187.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="ACHUZQ_001457"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="ACHUZQ_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="ACHUZQ_001458"
     CDS             18711..19823
                     /locus_tag="ACHUZQ_001458"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR1025188.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            19820..21163
                     /locus_tag="ACHUZQ_001459"
     CDS             19820..21163
                     /locus_tag="ACHUZQ_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1025189.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            21166..21720
                     /gene="pduT"
                     /locus_tag="ACHUZQ_001460"
     CDS             21166..21720
                     /gene="pduT"
                     /locus_tag="ACHUZQ_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR1025190.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21720..22070
                     /gene="pduU"
                     /locus_tag="ACHUZQ_001461"
     CDS             21720..22070
                     /gene="pduU"
                     /locus_tag="ACHUZQ_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR1025191.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22075..22518
                     /locus_tag="ACHUZQ_001462"
     CDS             22075..22518
                     /locus_tag="ACHUZQ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1025192.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22619..22948)
                     /locus_tag="ACHUZQ_001463"
     CDS             complement(22619..22948)
                     /locus_tag="ACHUZQ_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1025193.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23120..24178)
                     /locus_tag="ACHUZQ_001464"
     CDS             complement(23120..24178)
                     /locus_tag="ACHUZQ_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1025194.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="ACHUZQ_001465"
     CDS             complement(24376..24849)
                     /gene="sbmC"
                     /locus_tag="ACHUZQ_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR1025195.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="ACHUZQ_001466"
     CDS             complement(24968..26134)
                     /gene="dacD"
                     /locus_tag="ACHUZQ_001466"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR1025196.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26343..27770
                     /gene="sbcB"
                     /locus_tag="ACHUZQ_001467"
     CDS             26343..27770
                     /gene="sbcB"
                     /locus_tag="ACHUZQ_001467"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1025197.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27813..28040)
                     /gene="tsuB"
                     /locus_tag="ACHUZQ_001468"
     CDS             complement(27813..28040)
                     /gene="tsuB"
                     /locus_tag="ACHUZQ_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1025198.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28054..29112)
                     /gene="tsuA"
                     /locus_tag="ACHUZQ_001469"
     CDS             complement(28054..29112)
                     /gene="tsuA"
                     /locus_tag="ACHUZQ_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1025199.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
BASE COUNT         7689 a         7638 c         6936 g         6863 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgat agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaaacgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa ggcaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccgat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcgg ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatatcg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcga ggagttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg gttaactacc aacagctcgc
    20701 cacgccgcaa ctgttactga cagcgctcac ctgttcagag tgcaacgtct gcgccagtgt
    20761 cgcctgtccg gtaggaattt caccaatgcg cataaaccgc ctgcttaagc aggaattacg
    20821 cgcacaaaat ctccgctacg aaggtgcctt aaatcctgca gatcccatgg caaattatcg
    20881 actgattccc gtaaaacggc tggtaaccag acttggctta accccgtggt atcaggatgc
    20941 gccattgtct gaacaggtgc ctcagcccga aaaagtgacg ttattgttac gccagcacat
    21001 cggtgccagc gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg ggcaatgtgt
    21061 aggtcaaatt ccacacggca cattaggtgc ccccattcat gccagtattg atggcatggt
    21121 cagcgatgtg acagaaaacg cgattacgct tgtgagaggt taaccatgtc gaaagcggta
    21181 ggtattttag aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc catgctgaaa
    21241 agcgccaacg ttgagttact ggtcagcaaa acgctatgcc ccggaaaatt tctgctgatg
    21301 ctcggtggcg atgtgggcgc ggtgcaacag gccatcaaca gcggagcgtc ccttgctggt
    21361 gaaatgctgg tcgacagtct tgtcctgccg aatattcatc ccagcctcct ttctgcaatc
    21421 agcggactta accatgttga acagcaccgg gctgtcggcg tagtcgaaac ctggagtgtc
    21481 gccgcctgca ttgacgctgc cgattgtgct gtcaaagctg caaatgtcat tcttgttcgc
    21541 gtacatatgg cttttggcat tggcggtaaa tgctacatgg tggttgctgg cgatatttca
    21601 gatgttaata acgcggtctc tgccgccagc gtgcgcgcgg gggaaaaagg tttgttggtc
    21661 tatcggtcgg tgattgcccg tccacatgaa gccatgtggc gtcagatggt ggagggataa
    21721 tgacaactca gcacacaact gaacggatga ttcaggaata tgtcccaggt aagcaggtca
    21781 cgctggcaca tgtcatcgcc aatccaggga aggatctgtt taagaaattg ggtttaccag
    21841 acaccatttc cgctattggt attctgacaa ttacccccag cgaagcatct atcatcgcat
    21901 gcgatatcgc cacaaaatca ggcgctgtag agatcggttt tcttgatcgt tttaccggtg
    21961 ccgtagtgct gactggtgat gtttctgcga tcgaatatgc acttagccag gttacccgta
    22021 cgctgggtga aatgatgcgc tttaccgcct gccccattac gcggacctga cgttatgcaa
    22081 cgtatgatgc tcatcggccc cagccagtgc ggaaaaacgt cactcacgca atgtctgcgt
    22141 ggcgaaaata ttcattatca gaaaacgcag gcaatcgtct ggtccccgac caccattgat
    22201 acaccaggcg aatacctcga aaaccgcagc ctctacagtg cactgttggt cagtgcctgc
    22261 gaagcggaca ttatcgcgct ggtgcttaat gcggacgcac cgtggtcacc tttttcgcca
    22321 ggctttacct cgtcaatgaa tcgcccggtc attggagtag taaccaaagc agatttagcc
    22381 aacatggaac aaatatcttt ggtgaaatgt tggttacggg aagctggagc gcacaacgtg
    22441 ttagtaacca gtgcggttaa taaccatgga gttgcagaac ttttagcctt gctgcataca
    22501 gaagaaggct gtcgctaatt tcgacaggtt atcagatggg aatgatgacg gtgatacaca
    22561 gaaaagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa aacaggaatt
    22621 acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag atatcgcggc
    22681 gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct tcatagtcac
    22741 ctttcatgct ggcgatgatg ccctggattg cttcaacgct catcccaggc ttgatgtaat
    22801 cgctcaggtt gtccagcagc aggacgcgct tctggttgtc acggatcttt ttctcaacgt
    22861 cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat tccagtacgt
    22921 cctggaatga aggcttggta gtttccattt ttatacccct gataatgtga gagtcagatt
    22981 cgtttaagca accgctattc gttagggcca acattactca tagctgccgc agatgacaat
    23041 gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc agcagcaaga
    23101 tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc gatagcaact
    23161 caagctgatg cgccgtttcc atgttcagcc acacataacc atagattggt gtttccacta
    23221 ccttcaggtc atggtggtta ttgagcaact gacgcagctc ttccacagca tcgttcaatt
    23281 tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga acaaagctca
    23341 gcagtatttg ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc aataacacga
    23401 aatggctggg gcgcgtggcc cagtacgcat tgatttgcaa ctccagcata caaaccagat
    23461 tgcggttaat ggtctggata ccttcatata tcgattttgg aatacgggtt tctttgctgg
    23521 cgggcgcaat cagcccacgc attttcacgg catcggtcag gagtttttgt agatggcttt
    23581 ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga ttgatagacc cgattatact
    23641 cggtcagact tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt ggccagatac
    23701 cggtaaacaa cattgccagt aaagagccga ggatcacatc gccgcttcgc cataacgccg
    23761 tatcaatttc acctgtcggg gaaccaacaa caattgccag cgtcacccca atcaataatc
    23821 cttgatacgg tttcttgccc agcgccagcc aaccgcaaag aaacatcgcc gccgcgcacc
    23881 agactaacat cagcggtaac gaaattaact ccagttgcag agcgataaga cctaaaatcg
    23941 aacccaacac cgtaccgcca atacgttcga aggcacgagg gacaacgtta ccccagaacg
    24001 agattggccc cataatcacc accatggtga ccagcggcca ggtgctttcc gggatagtaa
    24061 acaggcggat aatgagaaaa gtgagcagga atgccagcgc gacccgagta ccatgcacta
    24121 tgcggtaatg gcggtatacc cggatttcaa acgggcttaa tgacttatcg gcacgcacgc
    24181 agaaactccg taatgaagat taaaagcgct cataactcat ctgtcggcca gaaggcgcgc
    24241 ctctgaatta tccgacggac aaaccgggaa cagtgtaatt tcccggcagg agcgaaaaaa
    24301 ttgaaaggcg catcttactc ttttcgcttt caaaaaaaga gtggtcatcg cgttaacaca
    24361 ccgccctgag atgaattagt gatgttttgg ctgcaccgca acatacattt cgatatccca
    24421 gtacccatct tccgcgccat tgttcaaata aacctcgaag cagggctttg gtaacatttc
    24481 ataagcactg tcctgcaaga ggctattaaa gaactgatac cagggtttag caaaatcatc
    24541 accgactaca cgagccaccg ccaccgcata ctgaccacct gaaatttctg tcagaatgac
    24601 gccctcactg ttttcgggaa gcgtaaagtt attcggtacc gtcacaacgg tgtcgcagcg
    24661 taatttttcg gcgggtgttt catctggatt gtcgtaatag acagcaaccc actccttcgg
    24721 cacaatattt ttgttatcta cccacatcat caactgctca aagcctttct ttaccgtctg
    24781 ttcccacggg ccaacgagat ggaaacctgc aacggtacgt ttctcttcct gcgtaatctc
    24841 gtagttcatg acgcctccat taacactgtt tttatataca gcatagttgc aatattaaaa
    24901 ccacaaggaa tgagtgttga ttatgcgagc agactcgcac tcctgccagt ctgctgcaaa
    24961 agaaaggtca ggccttatgg tggaaataat cactcaggcg ggaaaacatg ccgccttccc
    25021 cgacagattc cagggtaacc agcggccagt gcgccacctg tttatcacgg tcgtaaagtt
    25081 caatttcccc tacccgctga tgggcgctaa ttggcgcggt gagctcttta ccatcaaggg
    25141 tatatttggc tttgatatgt ggaatttcgg ctttcggtag caccatccag aactcttgtt
    25201 ccgttcctag gtcgatattt tctttatcgc cataccagat gcgttccgtt ccgacctttt
    25261 tcccacggtg caaaatttgc accgtagtaa agttttgttg cccccaacgc agtaattttc
    25321 ttgcctcttc ctcacgacct tttgcactgt cagcccccat taccactgca atgagacgac
    25381 gctgcccatc tacagccgaa gcaatgagat taaacccggc accagaagta tgacccgttt
    25441 tcaggccgtc aacattcatg gttttatccc acaacaaccc gttacggttt tgctgggtga
    25501 taccgttcca ggtgagactt ttctcactgt acatatgata aaactcgggc tcgccgtgga
    25561 tgatagcgcg agaaagcaca gctaaatcat aagccgagct atgctggcca ggtgcatcca
    25621 gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca tagttgttca
    25681 tcatttcaac aaactgccgt tgcccaccgg caatatagtc agccagagca acacaagcgt
    25741 catttccgga atccacaatt aaaccacggc ttaaatcacg taccgatacg cgatcgccct
    25801 ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt atctttcgcc cacgcatcgc
    25861 gccccacggt gacaatatcg tctggcgtaa tgcgatgact atcgatagcg cgatccacga
    25921 cataacccgt catcagcttt gtcaggctgg cgggattgcg ctgttgatgc tcattacccg
    25981 cggtgaggat ctgaccggtg gtgtaatcca tcaataccca ggacccggca tgaatctctg
    26041 gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc agacgataag ttaaaaacga
    26101 acaaagaagc agcaataata agacggcgtt tcaacagcaa accctcagga gtttcaaata
    26161 gctgttcttt ttacggaaat acttatgaac tggctggaat aaagtgcaag aaaatgtgac
    26221 taccctctca tttttatctg acatgatctg ttgccactcg ctgccaaatt gtggcgctaa
    26281 agctgattag cacggtgata tttgatactc tggcagacag cagaaataac ggatttaacc
    26341 taatgatgaa tgacggtaag caacaatcta cctttttgtt tcacgattac gaaacctttg
    26401 gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc gatagcgaat
    26461 tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgctgatgac tatttacccc
    26521 agccaggagc cgtattaatt accggtatta ccccgcagga agcacgggcg aaaggagaaa
    26581 acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag acctgtattc
    26641 tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt tatcgtaatt
    26701 tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat ttgctggatg
    26761 ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa aatgatgacg
    26821 gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggaattgaa catagcaacg
    26881 cccacgatgc gatggctgat gtgtacgcca ctattgcgat ggcgaaactg gtaaaaacgc
    26941 gtcagccacg tctgtttgat tatctcttta cccatcgtaa taaacacaaa ctgatggcgt
    27001 tgattgatgt tccgcagatg aaacccctgg ttcacgtttc cggaatgttt ggggcatggc
    27061 gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc agaaaatcgc aatgccgtaa
    27121 ttatggtgga tttggcagga gacatttcgc cattactgga actggatagc gacacattgc
    27181 gcgagcgttt atatactgca aaagccgatc ttggcgataa cgccgccgtt ccggttaagc
    27241 tggtgcatat caataaatgt ccggtgctgg cccaggcgaa tacgctacgc ccggaagatg
    27301 ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctgaaaatt ctgcgtgaaa
    27361 atccgcaagt gcgcgaaaaa gtggtggcga tattcgcgga agccgaaccg tttacgcctt
    27421 cagataacgt ggatgcacag ctttataacg gctttttcag tgacgcagat cgtgcagcaa
    27481 tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc acttttgtcg
    27541 ataaacggat tgaaaagctg ttgttcaatt atcgggcacg caacttcccg gggacgctgg
    27601 attatgccga gcagcaacgc tggctggagc accgccgcca ggtcttcacg cccgagtttt
    27661 tacaaggtta tgcagatgaa ttgcagatgc tggcacaaca atatgccgat aacaaagaga
    27721 aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa tttgaagcct
    27781 ccgccgctgg tacggcggag gtcaatgttg agtcaggctt tttgaacggt gatactccac
    27841 gctgcatcgc caatctgctg ataatcggtg atggcatgcc cttcttctgc cgcccactgt
    27901 gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc gccgctggcc
    27961 atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg cgtcaccaca
    28021 tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag ttgccagtcg
    28081 cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc cgagaatcat
    28141 aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca gtccattacc
    28201 gataaagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac ctaacccgct
    28261 acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg ctttggctgc
    28321 aataaatgac cccacgaaga tccctaacac caggaaaacg ccccagttaa tgtatttcac
    28381 gtcacccgcg accagaaatt gcagaatatt ggccgttggg gaagtgattc ctaacccaaa
    28441 catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac cgatgagtac
    28501 agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc cagttcggcg
    28561 cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca ccacccagag
    28621 cgttatcacc agcaaaacgg caaccaacgg ccaaacagac aaattaaaag tctcagcaat
    28681 agagttatgt tcagtactgt agtgctgcaa ggtttgattt aaaccactgg catgtggaga
    28741 acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc cgatcaatcc
    28801 ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa ttcccagccc
    28861 gaagatatac ccacctataa cagtaccaag ccacgggaac gcctcggctt cgtaagtcag
    28921 taggcccgcc tgaattaacg caaaaacccc cacgctttgt accgaaattg caatcagcaa
    28981 ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac ctgtcaggca
    29041 aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc cgcttaatat
    29101 cattgaaaac ataaattaat taacca
//
LOCUS       DAZPBM010000036        28894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPBM010000036 DAZPBM010000000
VERSION     DAZPBM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.036"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHUZQ_001470"
     CDS             complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHUZQ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR1025200.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
     gene            1753..2310
                     /gene="pinE"
                     /locus_tag="ACHUZQ_001471"
                     /pseudo
     CDS             1753..2310
                     /gene="pinE"
                     /locus_tag="ACHUZQ_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2649..3497)
                     /locus_tag="ACHUZQ_001472"
     CDS             complement(2649..3497)
                     /locus_tag="ACHUZQ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1025201.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHUZQ_001473"
     CDS             3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHUZQ_001473"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1025202.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4399..4902
                     /gene="tadA"
                     /locus_tag="ACHUZQ_001474"
     CDS             4399..4902
                     /gene="tadA"
                     /locus_tag="ACHUZQ_001474"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1025203.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHUZQ_001475"
     CDS             complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHUZQ_001475"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1025204.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6713..10600
                     /gene="purL"
                     /locus_tag="ACHUZQ_001476"
                     /gene_synonym="purI"
     CDS             6713..10600
                     /gene="purL"
                     /locus_tag="ACHUZQ_001476"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1025205.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11158..12585
                     /gene="qseE"
                     /locus_tag="ACHUZQ_001477"
     CDS             11158..12585
                     /gene="qseE"
                     /locus_tag="ACHUZQ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1025206.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12750..13463
                     /gene="qseG"
                     /locus_tag="ACHUZQ_001478"
     CDS             12750..13463
                     /gene="qseG"
                     /locus_tag="ACHUZQ_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1025207.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13453..14787
                     /gene="glrR"
                     /locus_tag="ACHUZQ_001479"
     CDS             13453..14787
                     /gene="glrR"
                     /locus_tag="ACHUZQ_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1025208.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14848..15186
                     /gene="glnB"
                     /locus_tag="ACHUZQ_001480"
     CDS             14848..15186
                     /gene="glnB"
                     /locus_tag="ACHUZQ_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1025209.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHUZQ_001481"
     CDS             complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHUZQ_001481"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1025210.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16749..18002
                     /gene="glyA"
                     /locus_tag="ACHUZQ_001482"
     CDS             16749..18002
                     /gene="glyA"
                     /locus_tag="ACHUZQ_001482"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1025211.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18200..19393)
                     /locus_tag="ACHUZQ_001483"
                     /pseudo
     CDS             complement(18200..19393)
                     /locus_tag="ACHUZQ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19511..22788
                     /locus_tag="ACHUZQ_001484"
                     /pseudo
     CDS             19511..22788
                     /locus_tag="ACHUZQ_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22885..23868
                     /locus_tag="ACHUZQ_001485"
     CDS             22885..23868
                     /locus_tag="ACHUZQ_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR1025212.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23891..25402
                     /locus_tag="ACHUZQ_001486"
     CDS             23891..25402
                     /locus_tag="ACHUZQ_001486"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1025213.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25427..26425
                     /locus_tag="ACHUZQ_001487"
     CDS             25427..26425
                     /locus_tag="ACHUZQ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1025214.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26458..27552
                     /locus_tag="ACHUZQ_001488"
     CDS             26458..27552
                     /locus_tag="ACHUZQ_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1025215.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27564..28436
                     /locus_tag="ACHUZQ_001489"
     CDS             27564..28436
                     /locus_tag="ACHUZQ_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1025216.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28484..>28894)
                     /locus_tag="ACHUZQ_001490"
     CDS             complement(28484..>28894)
                     /locus_tag="ACHUZQ_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1025217.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6866 a         7143 c         7864 g         7021 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gtgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggagaatg cccgggaatt ctcctcggtg
      241 gaaaaagaag tccattgtgt agaaacctcc cgacgatctg cttcaggaaa ccaggctgcc
      301 acagtttcgg gaagaggggg gatctgaaag ttctgccccc cttcagtgat gactctgata
      361 tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa acagtctgtt ttggtctgac
      421 agggaaatac gaaactgagg cccgttaaaa tttggaaccg aagtttgctg tattaagaat
      481 agcagagttt gatctgataa ggggttattt gcaatctcaa tagttatatg aggatctaaa
      541 cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt caaaataaaa aaatcttctc
      601 atgataatat tttcttggat gtttggtaag ttgcttaatc tattgctata tgcaaaaaga
      661 agttgcagtc tcctaggtaa agggggcagt tcagtaagat ggttgtgcgc aaccttaagt
      721 atcttaagat tagaaggcag agatggaaga gtttgcagtt cgttgttcct aacctcaagt
      781 ttttttaaat tcaagggtaa tgaaggcagt gtggataacc tattatactc tgcgcttagt
      841 gatttgatgg tgtctggtaa taacggcaga ttctctaatt gattttcgga cacatccaaa
      901 gttattagat aatgtggtaa tgcagatata tgtgttaaga aattatctct tgcaataagt
      961 tctttaatat tttcaggtaa atcagtaagg atggttaatt tattacagct cacatcaagc
     1021 tttttaatgc aagcagggag tacaggtaac gaggaaagcg ccaaggctcg caggtccagt
     1081 ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt acgctgttct
     1141 tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt gggggatgta
     1201 attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt aatttctcgc
     1261 atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc ctaatgagga
     1321 gggtgattca gaatattcgc cataagggaa tgagtgttat atcatgcttt ctaccctggt
     1381 aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt acatattcac
     1441 aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca acatctccaa
     1501 ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa tcatgtataa
     1561 aataagctta tatacatagt aacttcagca aaggatttag taatgatcca aatcatatct
     1621 caatgtactg tttttttctt taaaaaaaca gtacattcta ctttataatg actgtgtatt
     1681 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
     1741 gggggattta ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
     1801 ctacaacgta atgcgttgaa ctgtgcagga tgcgagctga tttttgaaga caagataagt
     1861 ggcacaaagt ccgaaaggcc ggggctgaaa aaactgctca ggacattatc ggcaggcgac
     1921 acactggttg tctggaagct ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg
     1981 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgactgattc aattgatacc
     2041 agcacaccaa tgggacgctt tttcttccat gtgatgggtg ccctggctga aatggaacgt
     2101 gaactgattg ttgaacgaac aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt
     2161 ggcggtcgcc gtcccaaact ttcaccagaa taatgggcgc aggccgggcg actaattgca
     2221 tcaggagttt ctcgccagaa ggtggcgatc atctatgatg ttggcgaatc gacttttttg
     2281 tataagaggt ttccagccag ggttatataa aaccgcagac acggcgtata caagaacgtg
     2341 ctacggttga ctgaaaagtt aatgtggtat gtcacaggcc ttttgcgccg taaagccccg
     2401 tcctttaggg cgcggaggac gttaaagaaa gtggtgttct ttgtgttgct gaacttatgc
     2461 aaaatttttt tgcaccataa atccaacaac acattcaata acacattgct gtatcactat
     2521 gaaaataaat gtgtttttat ttgatttcac acatatcaca attgatgcat tttttagtga
     2581 ttaacaacgc cataaggaaa ctctcaaaac aacacaaacc gggcgggcat tgtacgcgca
     2641 ttctttactc agaccagttt tttcaccagc gcttcactat gacgaatacg ttctggtgcc
     2701 agttccagat cctgctgaat cagcgcaatg aacagcaaat ccgttaacat tccctgagcg
     2761 tgacaagcag agattgaagc actgtttgtc gcttgttctt cggcaatggt atacaggcaa
     2821 tgagaagcac gctgttgcag ggcattcgga gtaaagccgg taatcgccag cacttttccg
     2881 cccactcgca gcatctcatc tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa
     2941 atggctaata acagatcgtc aggggacgac gcctgtactg ttgcgagtag cgcatgcata
     3001 tcgcgcactg cggcagcatt gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc
     3061 accagacccg aagcgccaat accggtcaga attatccgcc gcgcagagcg caacattgtt
     3121 acgcattcat gcagtttctc ttcactatta acgtttagcg ttgcgtacat agcggcagta
     3181 ttttctttaa tcagtttttc gccgaccagc cgtaacggat catcaccgcg gatttggtta
     3241 tgaatgggca cggagggcga ttccggctgg cttgccagcg cttcactcaa cgccaactta
     3301 agcgccggaa aacctttata gccgagtttt tgcgcgaact tcacgacgct ggactgactg
     3361 actccggctt cgttggccag ttgctgagag cttaaatggc gcgccgtatc aggttgtagc
     3421 agcagataat ccgccagttt tttatcactc tgggcaagcc cctggtaacg ctgacggata
     3481 cgaagtaagc cgttcatatc tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga
     3541 gacacacgcg agtgaatttt atattccatt gccgctaatt gccatgaatt aaaaattcca
     3601 gttagccatg atgcggtcga ttgttgccgg aaaagtgatg atctatctgt taggctatgt
     3661 agtagcgaaa attaatcagg atgtttcagt ccagaggagt atggtttggc aactcacgag
     3721 cgtcgtgtgg tgttttttga cttagatgga acattgcatc agcaggatat gttcggcagt
     3781 tttctgcgct atttactgcg tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta
     3841 ccgattatag ccattgcgtt attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg
     3901 cttctgtggg ggtgcacttt tggtcacagc gaagcacgtt tacagacgtt gcaggccgat
     3961 ttcgtgcgct ggtttcgcga caatgttacc gcctttccgc tggttcagga gcgattaacc
     4021 acctacctgt taagttccga tgctgatatc tggttgatta ccggctctcc gcagccgctg
     4081 gttgaagcgg tttatttcga tacgccctgg ctgccgcggg ttaatcttat cgccagccaa
     4141 attcagcgtg gctatggtgg ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc
     4201 gcacaactgg agcgcaaaat cggcactccg ctgcggctgt acagtggcta tagcgacagt
     4261 aatcaggaca atccgctgct ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt
     4321 gaactccagc aactggaata gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt
     4381 ataaccggag ttttcttttt gtctgaagtc gaatttagcc acgaatactg gatgcgtcac
     4441 gcgctgacgc tggcgaaacg tgcctgggat gagcgggaag tgccggtcgg cgcggtatta
     4501 gtgcataaca atcgggtaat cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc
     4561 accgcacatg cagaaatcat ggccctgcgg cagggtggtc tggtgatgca aaattatcgt
     4621 ctgatcgacg ccacgttgta tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg
     4681 atccacagtc gcattggtcg cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg
     4741 ggatctttaa tggatgtgct gcatcatccg ggtatgaatc accgagtgga aattacggaa
     4801 ggaatactgg cggatgagtg cgcggcgttg ctcagtgact tctttcgcat gcgccgccag
     4861 gaaattaaag cgcagaaaaa agcgcaaccc tcgacggatt aattttgttt ctcttcactc
     4921 cctttcctgg aaaacagcag agagggagaa tgggtcaaat agtttgatga cgactgggaa
     4981 agaaacgaga gaaaaggaaa tttctctttg cccaactccg taggcgatac cgccggataa
     5041 tcctgcgcca gttgcatcgc cgcttctgta gcctgcttct ctttctcttg cagataaccc
     5101 accaggctaa tctgatactt acgaatattt tcgacataag cgtaggcttc atgtccacga
     5161 gcgtagccgt aagtcagctt gctgtaatag ggtttctggc taagtaaagg cagacgctgt
     5221 tttacgtcag cccaactgtc aggattccct ttggtttttg ccgtcagggc gcgggcatcc
     5281 agcatatgcg catagcccat attgtacgca gcgagggcaa accagatccg ctcgttctcc
     5341 ggcacacttt ccggcacttt actcatcata tcctgcaaat aacgcacgcc accgctgatg
     5401 ctctgttcgg catcggtacg atccgtaatg ccgaggcttt gcgcggtatt tttggttaac
     5461 atcatcatgc cgcgcacacc cgtcggtgaa gtggcctgtg catcccagtg cgattcctga
     5521 taagcaatag cggccagcaa acgccagtca atttcttcgg cgtatttctc aaacaggggc
     5581 tttaactgcg gcagtaccgc atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca
     5641 aaatcatcgc catgccccag gtatttctct tcaatgcgtg ccagcgtaca gtcttcattc
     5701 atttcgttga agaagtcgag cagggcggcg gaaagggtat tatcgccatc taacgggcta
     5761 aaccaagtca ccggttgttc atcggtgata tcgagcgcta cggcgagctc cgggtgaacg
     5821 cgctgaaaca ggctgatggc gacagaatca gcaatggtgt aatcgagttt tccttcgatg
     5881 acatcttcca ttaattccgc agagcctttt ttgtcgtcta ccttccagct taattccggg
     5941 aattttgtgt ctttcagggt ttggagatcg ttaaccacca cgtgacccgg tgcaacggtg
     6001 agttgctccg ccgtcaggtt gcccagtgtg cgcgggcgat actgactcac tttataaacc
     6061 agttgttgtg acacggaata ataggtaggg ccaggctgat aattttttac ccgctcactg
     6121 ttatagacca gtcctgccgc cagcaggtcg gcgttaccat tatcgaggtc gtcaaacagc
     6181 tggctgatat tctgccgcac ggtcactttc agttttacac cgaggtaatc ggcaaactgt
     6241 ttcgccagtt cgtaatccag gccaaaaggt ttcccgttga tttcgttata agtcagggga
     6301 gtatgaatgg tgctcacacg caactctccc cgcgcttgaa tggcggcgat acggttgtcg
     6361 gctttaccaa accagggaat ggatggccag agagcgaccg cgagcagcag tgccagaatg
     6421 ccgatgaaca gataattaat ctttaatttt ttcaattagt taattctctg tgtcgtgcgc
     6481 gtcccagctt gaaaaaacgt aataatagtg aaaggtttac tcataaatga gcggcatttt
     6541 gcgtaaacct gcgccagatg gcaacttatt acagccattg gcggcacgcg ttgctaattc
     6601 acgatggtga ttttatttcc acgcaaacgg tttcgtcagc gcatcagatt ctttataatg
     6661 acgcccgttt cccccccttg ggtacaccga aagcttagaa gacgagagac ttatgatgga
     6721 aattctgcgt ggttcgcctg cactgtcggc attccgaatc aacaaactgc tggcacgttt
     6781 tcaggctgcc aggctcccgg ttcacaatat ttacgccgag tatgtccatt ttgctgacct
     6841 caatgcgccg ttaaacgatg atgagcacgc acaacttgaa cgcctgctga aatatggccc
     6901 ggcactcgcc agccacgccc cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac
     6961 catctctccc tggtcttcga aagcgaccga tattgcccat aactgcgggc tacaacaggt
     7021 aaaccgcctt gagcgcggcg ttgcttacta tatagaagcc ggtacgctga ccaatgaaca
     7081 atggcagcag gttaccgctg aactgcacga ccgcatgatg gaaacggtct tttttgcttt
     7141 agatgatgca gagcagttgt ttgcccacca tcaaccgact ccggttacca gcgttgattt
     7201 gctggggcag ggccgtcagg cgctgatcga cgctaacctg cgtcttggcc tggctctggc
     7261 ggaagatgaa attgactatc tgcaggatgc tttcacaaag cttggtcgta acccgaacga
     7321 catcgagctg tatatgttcg ctcaggcgaa ctctgagcac tgccgccaca aaatttttaa
     7381 tgccgactgg attatcgacg gtgaacagca gccgaaatcg ctgttcaaga tgatcaaaaa
     7441 tactttcgaa accacgccag atcacgttct ctctgcttat aaagataacg ccgccgtaat
     7501 ggaaggttct gaagtgggcc gctactttgc tgaccacgaa acgggccgct acgatttcca
     7561 tcaggaaccg gcgcatattc tgatgaaagt cgaaactcac aaccacccga cggcgatttc
     7621 tccgtggccg ggcgcggcga cgggttccgg cggcgaaatt cgtgatgaag gcgccaccgg
     7681 acgcggtgcg aagccgaagg ctggtctggt tggtttctcc gtttccaacc tgcgaattcc
     7741 tggcttcgaa cagccgtggg aagaagattt cggtaagcct gagcgcattg tcaccgcgct
     7801 ggacatcatg accgaaggcc cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc
     7861 ggcactgaat ggctacttcc gtacttatga agaaaaagtg aacagccaca acggcgaaga
     7921 gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga
     7981 tcacgtacaa aaaggcgaga tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc
     8041 aatgaacatc ggtcttggcg gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc
     8101 cgacctcgac tttgcttccg tacagcgcga caacccggag atggagcgtc gctgccagga
     8161 agtgatcgac cgttgctggc agcttggtga tgccaaccca atcctgttta tccacgacgt
     8221 cggcgctggc ggtctttcta acgcgatgcc ggaactggtg agcgacggcg ggcgcggcgg
     8281 taaatttgaa ctgcgcgata ttctaagcga cgaaccgggc atgagcccgc tggaaatctg
     8341 gtgtaacgaa tcccaggaac gctacgtgct ggcggttgct gccgatcaat taccgctgtt
     8401 tgacgaactg tgtaagcgtg agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga
     8461 actgcatctt tctctgcacg atcgtcattt tgataatcag ccgatcgatc tgccgctgga
     8521 cgtcctgctt ggtaaaacgc cgaagatgac ccgcgatgta caaacgctga aagcgaaggg
     8581 cgatgcgctg gtccgtgaag ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct
     8641 gccgaccgtg gcagagaaaa ccttcctggt gaccatcggc gaccgcagcg taaccggcat
     8701 ggtagcgcgc gatcagatgg tggggccgtg gcaggtgccg gtcgctaact gcgcggtcac
     8761 taccgccagc ctcgacagct actacggtga agcgatggcg attggcgagc gtgcgccggt
     8821 tgcactgctg gatttcgccg cctctgcccg tctggcggtc ggtgaagcgt taaccaatat
     8881 tgccgcaaca cagattggcg atatcaaacg cattaaactt tccgccaact ggatggcggc
     8941 ggcaggccac cctggtgaag atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga
     9001 gctttgtccg gcgctgggcc tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac
     9061 ccgctggcag gaaggtaacg aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc
     9121 tgcatttgcc cgcgtggaag atgtgcgtca caccatcacg ccacagcttt ctaccgaaga
     9181 taacacactg ctgctgattg atttgggcaa aggcaataac gcgctgggcg caacggcgct
     9241 ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat gtacgcgacg tcgcgcaact
     9301 gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag cgtaagctgc tcgcatacca
     9361 cgaccgctct gatggcggcc tgctggtaac gctggcggaa atggcctttg ctggtcattg
     9421 cggcattaac gcggatattg cctctctggg cgacgatcgc ctggcggcgt tgtttaacga
     9481 agaactgggt gcggtgattc aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact
     9541 ggcacagcat gggcttgctg attgtgtcca ttatgtaggg caggcggttt ccggtgaccg
     9601 ttttgtgatt accgccaacg ggcagactgt attcagcgaa agccgcacca cgttgcgtgt
     9661 ctggtgggca gaaactacct ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga
     9721 ccaggagcac caggcgaaat caaacgacgc cgatccgggc ctgaatgtga aactgtcgtt
     9781 cgatatcaac gaagatgtgg cagcaccgta tattgccact ggcgcacgtc cgaaagttgc
     9841 tgtgctgcgt gagcagggcg tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc
     9901 aggctttgat gctatcgacg tgcatatgag tgacctgctg accggacgca cgggcctgga
     9961 agatttccac gccctggtcg cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg
    10021 tgaaggttgg gcgaagtcaa tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt
    10081 cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa
    10141 cctgcgtgaa ctgatcccgg ggagcgagtt gtggccacgt tttgtgcgta acacctccga
    10201 tcgctttgaa gcgcgtttca gcctggttga agtaacccaa agcccgtctc tgctgttgca
    10261 ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat ggtgaagggc gcgtggaagt
    10321 gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg ctggtggcac tgcgctatgt
    10381 cgataacttc ggcaaagtca ctgaaaccta cccggctaac ccgaacggtt caccgaacgg
    10441 tattacggcg gttacgactg aaagtggtcg agtcaccatt atgatgccgc acccggaacg
    10501 tgttttccgt actgtcagca actcctggca tccggaaaac tggggcgagg atggcccatg
    10561 gatgcgcatt ttccgcaatg cgcgtaagca gttggggtaa gtcatcagtt cattggtttt
    10621 gcagcccgga tgcggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    10681 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    10741 aatgattgat tttattgtaa tctaataatt ttatcttaag ttgtctcttt ttagcgacac
    10801 agtggctgat ttacattcaa atagcgaaga agccataaat ccctcaaagc aacgtataat
    10861 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    10921 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    10981 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    11041 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    11101 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    11161 aaacgctggc ccgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    11221 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    11281 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    11341 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgaccct
    11401 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    11461 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    11521 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    11581 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    11641 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    11701 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    11761 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    11821 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    11881 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    11941 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    12001 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    12061 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    12121 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    12181 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    12241 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    12301 gaatccggta acatttgcct tcgcagcagt tcacatggtg cgcgggttta tattgatgtc
    12361 atcaatacag gcacgcccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    12421 cagggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    12481 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    12541 gtttgtttcc gcattgaatt accgtcgtcg aaaaacacga aataatgcta acagcctatc
    12601 ctatcaggct attttatttg tcagttcggc cccgggcagt gctcgaaatc ttcacgtacg
    12661 atatgtacgc tccggtttct ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc
    12721 cactgggata ggctttaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    12781 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagaatcaca
    12841 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    12901 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    12961 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc
    13021 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    13081 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt
    13141 taagtaccga gatcccggca caggttcgcc cactgtatca actctggcgc gacggtcagg
    13201 cgctgcaact gcaactggcg gaagagcgcc aacgttacag taaactccag cagtccagcg
    13261 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    13321 ccacccgcaa gctggaaaac ctgaccgata ttgaacgcca gctctcgacc cgcaaaccga
    13381 ctggaaattt ctcgccggat acgccgcacg agagtgaaaa gcccgcgcca tccacccatg
    13441 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    13501 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    13561 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    13621 ctgcggatgg atgaaatgga cggtatgcag ctatttgctg aaatccagaa agtgcagccg
    13681 ggaatgccag taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    13741 cagcagggcg tttttagttt cctcaccaag cctgtcgata aagatgcgct atatcaggca
    13801 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    13861 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    13921 gacgtcagcg ttttgattaa cggtcagagc ggcaccggga aagagatttt cgcccaggct
    13981 atccacaacg ccagcccgcg caacagcaaa ccatttattg ctattaactg tggcgcattg
    14041 cccgagcaat tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    14101 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gaacgctatt tctcgatgaa
    14161 attggcgata tgcctgcgcc gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    14221 gtgcgcccgc tgggcagtaa ccgcgatatt gatatcaatg tgcggattat ttctgccact
    14281 caccgtgatc tgccaaaagc gatggcgcgc ggggaattcc gcgaagatct ctattaccgc
    14341 ctcaacgttg tcagcctgaa aattccggca ctggcggagc gcacagaaga cattccgcta
    14401 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    14461 tctaccgatg cgatgaaacg cctgatgacc gcgagctggc cgggtaatgt gcgccagttg
    14521 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggtgattag tgatgcgctg
    14581 gtggagcagg cgctggaggg tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat
    14641 cagtttgaac tcaactattt gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac
    14701 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    14761 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    14821 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    14881 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    14941 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    15001 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    15061 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    15121 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    15181 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    15241 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    15301 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    15361 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    15421 ttagcgcgat cggcttcgct cggctgacga tgccaggtgt gcgcggtaaa gcgcggcagt
    15481 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    15541 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    15601 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    15661 ataaagaaat cacctgccgg agcgaccagt ttcacgacat cgccaacatt ggcgtgattg
    15721 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    15781 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    15841 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    15901 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    15961 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    16021 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    16081 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    16141 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    16201 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagggcttca
    16261 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    16321 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    16381 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    16441 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    16501 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    16561 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    16621 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    16681 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    16741 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    16801 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    16861 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    16921 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    16981 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    17041 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    17101 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    17161 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    17221 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    17281 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    17341 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    17401 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    17461 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    17521 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    17581 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga
    17641 tggtagaagt gttccttgag cgcggctaca aagtggtttc cggcggcact gataaccacc
    17701 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    17761 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    17821 tgacctccgg tattcgcgtg ggtactccgg cgattacgcg tcgcggcttc aaagaagcag
    17881 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    17941 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgtttcccg gtttacgcat
    18001 aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaac tttgcaaatt caatatattg caatatccgt gtaggcctga
    18121 taagcgtagc gcatcaggca atcttaggta tgtgatcatc aaggcttcct tcgggaagcc
    18181 tttctacgtt attgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa
    18241 tccccaacac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtt gctttcacgc
    18301 tcggcccttc gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc
    18361 agtgctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt
    18421 tgaggaagtt atacagactc aaaccaatgg cattggcact gcgatcaacc caactggtga
    18481 tccacggttc tccactctac caggcagcga ttaactgcgc tgtagtcagt ttttccggat
    18541 caagctgagt attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac
    18601 ttaacgaggc gacggtttcc aggcagccgt aacgtccaca gtcgcagacg acgccatccg
    18661 gattaacgat ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct
    18721 cgttaatcac gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt
    18781 tctgcgaatt attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca
    18841 tcacccgaat gccgagcttc tcttccagca gatacttcac ctcaaccggc gttgtccacg
    18901 gcgcttgcgg catggtttgc gacacgccgg tcaccggatc aacctgaccg tggattgcca
    18961 gcgccagatt gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga
    19021 tttcggacag cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt
    19081 tcggacttaa acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc
    19141 acagcgtcca gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct
    19201 ggctttcatc gtcgatattc accacccgtt tttcgctttc cagttcctgc aaaatattac
    19261 tgactgccgg aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt
    19321 gccggtacag cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa
    19381 tgcaggctct catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac
    19441 attgccagat tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat
    19501 tgagggcacc atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga
    19561 aaccgggccg caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc
    19621 cggcgcggtt tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc
    19681 ctggcaggcg gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg
    19741 cggtcgggtg catcgcgcgt gggataaagt gaaacagcgc gattttgttt atcacaatga
    19801 agtcattaaa cctgcgctgg tggggctgct ggggccgtgg atttccggtg ggattgagtt
    19861 taactggccg caacatcatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc
    19921 ccatgaagac ggtgcacaga cggtgtgggt aggcgaaacg gagccgatgc atggtttaca
    19981 ggtgatgaca ggtttcaccc tgcgccctgg gcggcgctgg aaatcgccag ccgcgtctat
    20041 aacggcaacg ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggt
    20101 gaagggcatc agagcgtttt cccaccggat ttgactgcgg tgtttgatca cggcaaacgg
    20161 gccgtctccg ctttccccat cgctaccggc acttactaca aagtggacta ctccgctgga
    20221 gtggacattt ctcgctataa aaatgtgccc gttccaacct catatatggc cgaaaaatcg
    20281 cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg gcggtttgct gcacgttgcc
    20341 aaccaccata ttgcgctagg taaaaaacag tggagttggg gacacagtga atttggccag
    20401 gcgtgggata agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt
    20461 tttgccgata accagcccga ttttacctgg cttgatgctt acgaagagaa gcgttttgag
    20521 cagtatttcc tgccttatca ttctctgggc atggtgcaaa atgcctcccg cgatgcggtg
    20581 ataaaactcc agcgtagtga gcaggggatt gagtgggggc tgtatgccat ctctccgttg
    20641 aacggatacc gcctggcgat ccacgaaatc ggcaaatgca acgcgttact tgatgatgcc
    20701 gtggcactga tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgatagg
    20761 ctgaccattg agctttccga tgccgacggc aatattgtac tgagttatca ggaacatcag
    20821 ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac tggcagcaca agacattacc
    20881 agtacagatg aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt
    20941 tcaccgttcg attactacct gcgcggcgtg gcgctggacc cgctggatta tcgctgtaac
    21001 ctggcgctgg cgatgctgga atataaccgc gcagatttcc cgcaagcggt ggcgtatgcc
    21061 agtcaggcgc tgaaacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt
    21121 ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc
    21181 tggcgtgcgg tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg
    21241 gcggcgcgta atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc
    21301 tgcccaacca atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt
    21361 caggacaacg cgcgtttgca gcgcgcgaaa ctgctgcgtg attatccgct gaacgccatt
    21421 ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt
    21481 ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga
    21541 atgcccactc tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt
    21601 tatctacaag ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt
    21661 gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag
    21721 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc
    21781 agctacaaca aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
    21841 gatggctggc gcgggttggc gatccatgcg tggaataagc aacacgatta tgaactggcc
    21901 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
    21961 cgggatttgc tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa
    22021 aataatctgg aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg
    22081 catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg
    22141 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
    22201 tggcagcatc ttgatgccag acagccgcag caggcctgcg aactgcttca tgccgcgctg
    22261 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
    22321 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgac gcgttgttta
    22381 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
    22441 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acaaatcgca
    22501 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
    22561 gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga tttacaacag
    22621 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
    22681 gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
    22741 gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac cctattaact
    22801 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
    22861 aataaaaact ctacaggagg cactatgcct aaaaaaatga gaacaacccg taatctatta
    22921 ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa agaaatgacc
    22981 attggtgcaa tataccttga taccctggga tattacgctg gagtgcgtca gggcgttcag
    23041 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
    23101 atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt
    23161 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
    23221 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
    23281 gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc tgccgccgat
    23341 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
    23401 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc
    23461 gcgcaaattg tcgctaatca ggaagggact gttttaaata aagcgatttc cgttggtgaa
    23521 aaactgatta tctccacgcc ggatctcaac gccattatgg gggagtcggg cggtgcgaca
    23581 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
    23641 tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta
    23701 gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt gaaggttatc
    23761 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
    23821 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
    23881 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
    23941 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
    24001 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
    24061 cggcagcgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
    24121 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggaatt
    24181 aagtctggtg gaagggctga cggtggcaga aaacctctgc cttggtcaat ggcctcgccg
    24241 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
    24301 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
    24361 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacccac
    24421 cagctcgctt gccagtgcag aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc
    24481 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaatccgcc gcattgcctc
    24541 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
    24601 cacgcatcat attgtgtcgc taatgctagg gcgtgatcac gttgatattg cgccggttgc
    24661 ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc ataagcccaa
    24721 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
    24781 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg
    24841 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
    24901 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
    24961 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag tgctgcaatg
    25021 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgctag
    25081 tagcgaaacg cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg
    25141 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
    25201 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
    25261 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt
    25321 acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
    25381 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
    25441 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
    25501 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
    25561 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
    25621 tgccgcctgg gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc
    25681 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
    25741 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
    25801 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
    25861 cggaatgggg ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct
    25921 ggactggctg ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt
    25981 gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt
    26041 tggcggtaat gccacggcgg cgcagttgtg cggtattaac gttcgtcggg tgcgcattct
    26101 tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgctgg cggcacgcct
    26161 cggttccggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt
    26221 ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt
    26281 gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca
    26341 gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca
    26401 gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg
    26461 aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca
    26521 ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta
    26581 ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa
    26641 cactgtgcca cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat
    26701 gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac
    26761 cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt
    26821 cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat
    26881 gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac
    26941 gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc
    27001 gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg
    27061 atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa
    27121 cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc aaccaccgaa
    27181 ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc gctcgattgt
    27241 tctggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg gggacgggtg
    27301 gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat
    27361 caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccac
    27421 gatttgactg actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa
    27481 caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac
    27541 ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc
    27601 tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct
    27661 tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat
    27721 ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg gcgtgagtat caactgcaac
    27781 cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat
    27841 gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc
    27901 actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg acgctctctg
    27961 tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt
    28021 tgtcaccaca aacgcggatt caggcgcagg cgagcggtta ctggctggaa cgggagcagt
    28081 ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc
    28141 cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg
    28201 agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt
    28261 tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg
    28321 aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc
    28381 tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg ctgtaattta
    28441 ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag
    28501 aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct
    28561 tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac
    28621 caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa
    28681 agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca
    28741 gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca
    28801 ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca
    28861 aaacggggcg ggcagctcca aaatcaaaat aacg
//
LOCUS       DAZPBM010000037        28241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPBM010000037 DAZPBM010000000
VERSION     DAZPBM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.037"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..645)
                     /locus_tag="ACHUZQ_001491"
     CDS             complement(<1..645)
                     /locus_tag="ACHUZQ_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1025218.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLL"
     gene            complement(745..1656)
                     /gene="gsiD"
                     /locus_tag="ACHUZQ_001492"
     CDS             complement(745..1656)
                     /gene="gsiD"
                     /locus_tag="ACHUZQ_001492"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1025219.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1659..2579)
                     /gene="gsiC"
                     /locus_tag="ACHUZQ_001493"
     CDS             complement(1659..2579)
                     /gene="gsiC"
                     /locus_tag="ACHUZQ_001493"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1025220.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2597..4135)
                     /gene="gsiB"
                     /locus_tag="ACHUZQ_001494"
     CDS             complement(2597..4135)
                     /gene="gsiB"
                     /locus_tag="ACHUZQ_001494"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1025221.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4155..6026)
                     /gene="gsiA"
                     /locus_tag="ACHUZQ_001495"
     CDS             complement(4155..6026)
                     /gene="gsiA"
                     /locus_tag="ACHUZQ_001495"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1025222.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6013..6978)
                     /gene="iaaA"
                     /locus_tag="ACHUZQ_001496"
     CDS             complement(6013..6978)
                     /gene="iaaA"
                     /locus_tag="ACHUZQ_001496"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1025223.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7182..8417
                     /gene="moeA"
                     /locus_tag="ACHUZQ_001497"
     CDS             7182..8417
                     /gene="moeA"
                     /locus_tag="ACHUZQ_001497"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1025224.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8417..9166
                     /gene="moeB"
                     /locus_tag="ACHUZQ_001498"
     CDS             8417..9166
                     /gene="moeB"
                     /locus_tag="ACHUZQ_001498"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1025225.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9345..10007)
                     /gene="fsa"
                     /locus_tag="ACHUZQ_001499"
     CDS             complement(9345..10007)
                     /gene="fsa"
                     /locus_tag="ACHUZQ_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1025226.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10138..11037
                     /locus_tag="ACHUZQ_001500"
     CDS             10138..11037
                     /locus_tag="ACHUZQ_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1025227.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11043..13475
                     /locus_tag="ACHUZQ_001501"
     CDS             11043..13475
                     /locus_tag="ACHUZQ_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1025228.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13621..14436
                     /gene="ybiV"
                     /locus_tag="ACHUZQ_001502"
     CDS             13621..14436
                     /gene="ybiV"
                     /locus_tag="ACHUZQ_001502"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1025229.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14588..15853
                     /locus_tag="ACHUZQ_001503"
     CDS             14588..15853
                     /locus_tag="ACHUZQ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1025230.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15820..16980)
                     /locus_tag="ACHUZQ_001504"
     CDS             complement(15820..16980)
                     /locus_tag="ACHUZQ_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1025231.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17218..18519
                     /locus_tag="ACHUZQ_001505"
     CDS             17218..18519
                     /locus_tag="ACHUZQ_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1025232.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18534..20897
                     /locus_tag="ACHUZQ_001506"
     CDS             18534..20897
                     /locus_tag="ACHUZQ_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR1025233.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21077..22672)
                     /locus_tag="ACHUZQ_001507"
     CDS             complement(21077..22672)
                     /locus_tag="ACHUZQ_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1025234.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22891..23811
                     /gene="ldtB"
                     /locus_tag="ACHUZQ_001508"
     CDS             22891..23811
                     /gene="ldtB"
                     /locus_tag="ACHUZQ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1025235.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23870..24988)
                     /locus_tag="ACHUZQ_001509"
     CDS             complement(23870..24988)
                     /locus_tag="ACHUZQ_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1025236.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24985..25452)
                     /gene="mntR"
                     /locus_tag="ACHUZQ_001510"
     CDS             complement(24985..25452)
                     /gene="mntR"
                     /locus_tag="ACHUZQ_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1025237.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25638..25766
                     /gene="mntS"
                     /locus_tag="ACHUZQ_001511"
     CDS             25638..25766
                     /gene="mntS"
                     /locus_tag="ACHUZQ_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1025238.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26038..27621
                     /gene="opgE"
                     /locus_tag="ACHUZQ_001512"
     CDS             26038..27621
                     /gene="opgE"
                     /locus_tag="ACHUZQ_001512"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1025239.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27670..28185)
                     /gene="ompX"
                     /locus_tag="ACHUZQ_001513"
     CDS             complement(27670..28185)
                     /gene="ompX"
                     /locus_tag="ACHUZQ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1025240.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         6887 a         7776 c         7076 g         6502 t
ORIGIN      
        1 taacaaatcg tgatctttgc cgttgatacg tagcgtaaaa gagatacatt ttttacccgg
       61 acgatcaaag caattgtatg ccggattcca gctgttgtta gagtcatctg aagccaccga
      121 ccacggtcgt ttcgatgggt taaaatcggc aaaaataacc cgccctgact cgtcgacaag
      181 cggtccattt acttgttcct gatgataaac aacgatttta tcattcgccc tccgcgaaaa
      241 gagataatat ttattatcat caagtaccag tcccaccgag tctaaaaatg gcatcatttc
      301 cattttcaga atcacggcgc gttgaatctc ctctgtattt tttaataaat acagtgagtc
      361 ctttaatgtc agggccgaac gttcaatatc tttaaagaaa tagccaacgt ttttctgaat
      421 aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat
      481 gatgaaaaaa agaccaataa atgacagtgc tgccaataaa aataaaatta tcagccttat
      541 ctttatcttt tcgtataaac tcagcatgtt aatacattta acaaaataac tatctgaacg
      601 ctgtgccgct gcaaaaatag tcccttgctg cggcttttta accaaattac ccgattctac
      661 aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga ggtgattacc
      721 ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg catcgcgtaa
      781 accatcgccc aacaaattga acgccagtac ggtcagaaaa atagccaggg ccggaaaaac
      841 agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg ctccccactc
      901 tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag agataatcga
      961 ggtgccaatg cgcatggtga aaaacaccac gatagacgag acggtcccag gcaagatatg
     1021 acgcaataaa atggtcatat cgctggcacc aatactgcgt gctgactcaa taaaggtttg
     1081 ctgtttcaac accagcgtgt tgccgcgcac cagtcgggca aacgcgggga tggaaaaaat
     1141 ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac accgcaacaa cagcgatcgc
     1201 cagtaaaata cccgggaagg caaacagcac atcgcaaatg cgcatgatca gccgatccca
     1261 ccagccttca taatatccag ccaacaagcc cagcaacgtc ccgatcgccg caccgataaa
     1321 cacggcaaac acgcccgccg ccagcgagat ttgcgcacca accaggacac ggctgaaaat
     1381 gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa gaaggtccgt tattcagatt
     1441 gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa aaatggccac
     1501 cacaatcaat aaaataacga ataatgcggc ggtcatcgcc atatgctgac ggcgaaatcg
     1561 tcgccagaat tcatgccacg gtgtacgcac ctggtcaggt ttgaccagtg gcatggcgtt
     1621 taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct gatagccggg
     1681 ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc cagcgagaaa
     1741 agcagaattt ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc aacgagtaag
     1801 cgtccaagtc cgggccagtt gaaaactttc tccaccacga tagaaccacc gagcaaaaag
     1861 ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttacgtag cccgtgtttg
     1921 aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata atcttcgctt
     1981 aaaacatcga caaacgacgc gcgggtaaag cgcgccatca cggctgccac tgccgcgccg
     2041 agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt aggcagccag
     2101 cccagttcaa cggagaatac ctgaattaaa agcatcccca gcgcaaatgc cggaaacgag
     2161 atccccgaca ccgcaatggt catactcaat cgatccggcc aacgattacg ccagacggcg
     2221 gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt tatggtcagc
     2281 cacagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg cgacaccatc
     2341 gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg gtgatacagc
     2401 ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc agcttcgagc
     2461 ccggcaatca atcgcgccgg atcgccgggc agcatatgca caaataaaaa caccagcacc
     2521 gagacgataa acagcgtcgg aatcaacccc agtaagcgtt tgataacgta attaagcatt
     2581 ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg gtgtctggca
     2641 tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct accaccagcg
     2701 ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag cgggtctttt
     2761 ccgccggatc attagttttc agtgcctgag ccaggaagtc gtccacctgt ttattgctgt
     2821 aaaatgcggt attaaacagc gtcggtggcc agttttgcga ggcgaacagt ggcgatagcg
     2881 cccagtccgc ttcgccggtt gaagccgacc agccagtgta gaacatccgc acgccgctct
     2941 ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc gcggtcacct
     3001 gggctttaat cccgacctgc gctaactgct gctgggtaaa ttgcagcact ttctgcgcgg
     3061 tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat cccgcctctt
     3121 tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt tgcgcgtagg
     3181 cgatacttgg cggtaccaca ccagtagctg gcgttgcata gcccgcaaag gcaactttca
     3241 ccagcgccgg acggttaatg gcgtaattca gcgcctcacg gaccttcggg ttatcgaacg
     3301 gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg gccatcaact
     3361 cgatattttt gtttttctcc agcagtgtgg cctgctcgta gggaatgggg aaagcaaact
     3421 gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc ggacgccagg
     3481 ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt ttcaccttca
     3541 caaaatcggt ctgattccag gtatccagtt catacggtcc ggttcccacc ggatgaaaac
     3601 caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc gccggatggg
     3661 caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc gttgtcggat
     3721 cgatcgcctc cgttttggca atattcttat acaggttata gcgtttaaga tgattcgccg
     3781 gatcgctggc ccggtccaga ttcgctttta ccgccactgc gttgaaatcg gtgccatcct
     3841 ggaatttaat gccttcccgc aatttcacgg tgtaagtaat gccgtcatcg gaaacggtat
     3901 aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac agcccctggt
     3961 aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatacgga tcgagcgtgg
     4021 tgaaattcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa gatgccatca
     4081 acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt gccattttat
     4141 tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg tgcgacgtaa
     4201 tgccccggcc cgacgcattg caacgagacg gctgccacct cttcgccacg cagatgaata
     4261 ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga cggttcagcg
     4321 accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc gaagaccgcg
     4381 cgccgtggac caatttcaac aatttgcccg agatacatca ccgccacacg atgactaatc
     4441 cgctctacca cggccatatc gtgggagata aacagatacg caatgccgaa atcacgctgg
     4501 agatcgagca acaagttgat aatctgcccg cgaatagaaa catccagcgc cgaaacggct
     4561 tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca gatgcgctgg
     4621 cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa caggcccacg
     4681 cgctccagca accacgcaac gcgtgcagcc gcgtctttac ctggcaataa accgtgtaca
     4741 cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga agcgtaaggg
     4801 tcctgaaaaa taaactgaat atcccggcgc aatgcctgaa gtttgccggg tgacaaggta
     4861 tcgattctct gaccgttaaa gataatttcg ccgccctgcg attcgaccag gcgcagcaac
     4921 gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag cgtttcgcca
     4981 ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac gcgattcaac
     5041 aaaccgctgc gcaaagggaa acgggtgaca agattacgca ctcgtaaaac aggttcgcca
     5101 tccaccaccg ttttctgctc gatggggggg gcctgtttcg ctggatgttc aagcgatatc
     5161 aacgggaaac gtcggggata atccaaccct ttcatcgcac caagttgcgg aacagcagct
     5221 aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac ggtacccgtt
     5281 tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc caccacgccc
     5341 atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac tttgattaat
     5401 tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc ggcaatcagc
     5461 accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat cccgccagag
     5521 agttgatgcg gataacgtga aagaatggtt tgtgcctcag gaatgcgtac ctgatccagc
     5581 atccgcttcg cctcgaccat cgcttcttca cgactggcgt tctgatgcag acgaattgat
     5641 tcggcaatct gttcacccac agtaaatacc gggttcagcg atgtcatcgg ctcctgaaat
     5701 atcatcgcca tatccgcacc gcgaacatgg cgcatttgtg cagcgctctg ctcgctaagt
     5761 tcaatcactt cgcgactgcg ccgctgcaac agcattttat cgcactgtac taaaccgccc
     5821 gcctgttcca acaggcgcat caatgccaac gcagtcactg acttaccgga gccggattcg
     5881 ccaacaattg ccagcgtctc accgcgttgc agactaaaag agagattgcg gaccgcagct
     5941 attttctgct ggtcctgcat aaaggcaata ttcagatttt caaccgccag cacattaccg
     6001 gcatcaagtt catcactgtg tggcaacggt gtcccctttt tcacggtaga taccggtggt
     6061 tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa acggtagcgc
     6121 gacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg cagggagttt
     6181 ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat ccattaacgc
     6241 ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccga tacaagaaac
     6301 cgccacactg gcgttattgg cgtagcatcc ggcacccact aagggactat cgccaactcg
     6361 tccgggtaat ttattggtca ttccgcctgt ggacgtggct gccgccaaat tgccgtctaa
     6421 atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg gcgcaccgct
     6481 atggtcgagg acggttgccc cttccttgcg cgctgccagt agttgttcat aacgcaaagg
     6541 cgtggagaaa atctccggcg agacgcgctc catgccacga gcaaacgcaa aattttctgc
     6601 cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg cggcaagaac
     6661 cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt taccatccat
     6721 cacacaggcg tccagttcat gggtttcatc acgcgtaaag acagcgccaa ttccggcgtt
     6781 aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca gcgcactttc
     6841 gcccgcttcc agcattttct gcccggtttc aacaatggca gacaacgcct caatgtagcg
     6901 taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac catgaattgc
     6961 aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt ttgctataaa
     7021 tatcagaatt tttatgaatt actcattcaa tttttgaata tagaaagatg gatatggcat
     7081 gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
     7141 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
     7201 tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca ctgaccgccc
     7261 aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
     7321 cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg
     7381 atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtatc
     7441 atggtgaatg gcctgcgggc acctgcattc gtattatgac cggtgcgccg gtgccggaag
     7501 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
     7561 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
     7621 cggttgtttt cccggcggga actcgcctga ctaccgcaga gctgccagta attgcttcac
     7681 tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
     7741 atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgac accaaccgtc
     7801 tcgccgtaca cctgatgtta gaacagctgg gttgcgaggt aatcaactta gggattatcc
     7861 gcgacgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtag
     7921 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag
     7981 agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg
     8041 gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
     8101 ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc gccagcggcc
     8161 tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg
     8221 atttccagcg cggcgtgctg caacgcaacg ccgacggtga acttgaagtg acgaccaccg
     8281 gacatcaggg ttcacatata tttagctcct ttagtctcgg caactgcttt atcgtgctgg
     8341 aacgcgatcg cggcaatgtg gacgtgggcg aatgggtgga agtagaaccg tttaacgcgt
     8401 tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca
     8461 aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct
     8521 gatcgtcggc ctcggtggac tcggctgcgc agcttcgctg tatctggcaa gcgccggtgt
     8581 cggcaacctg acgctgctcg acttcgacac ggtttcgctc tcgaatctgc aacgccagac
     8641 actgcacagt gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtg acgccctgac
     8701 gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctagatg acgcagaact
     8761 tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg ttgcggtacg
     8821 taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
     8881 tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg
     8941 ccttagccgt ttgtttggcg agaacgcatt aacctgcgta gaagcaggcg tgatggcacc
     9001 gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc tggcaggtta
     9061 tggcaaaccg accagcggga aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga
     9121 aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc cacctccagg
     9181 cctgataaga cgcgacaagc gtcgcatcag gcatttgctc cagacgccag atgcggcgtg
     9241 aacgccttat ctggcctacg gttcgggcac agatttccaa gcgcggcagg catcggtgca
     9301 ctccgccacg tcatcagcaa tttatgagga tgcagaacgt ccggttaaat cgacgttctg
     9361 ccaaacgctc cctgccagtc ctgctcaaac ttcgccacag cggcttccac cgccggatag
     9421 ctaatcatct gttgtgccac atccagtggc agagtaattg attcacatcc tgccagtaag
     9481 cagtccagcg cctgacgcgg ggttttgaaa ctcgctgcca gcactttcgc ctgcggcgca
     9541 tgcattttca ataactggtg taagtcggtc acagtctgaa tgccgctacc gccctgagca
     9601 tcaatacgat taacgtaagg cgcaacatat tccgcacctg ccagcgccga cagcaaccct
     9661 tgtgctgcgc catataccgc ggttcccagc gttggaatcc cttccgcttt taacatctta
     9721 atagctgcca gcccctcggc ggtcaccgga actttcacca cgatatccgc aataatagaa
     9781 cgcaatttac gtgcgtcatt caccatccct tcagcagtgg tagccattac ctgggcaaac
     9841 agacgcccct gaccgcccat cgcttcatga agttgcggaa gcacaacatc cagcggtttt
     9901 ttacccgcgg cgataatgct tgggttagtg gtcacacccg ccagcggaaa aatacgtgac
     9961 agcgccttca ccgcaacaac gtctgaagta tccagataca gttccataac catcctcaaa
    10021 atgtactttt tgtctgaatg tagactatca cgggaaaact ctctgaacag ttgacgcaca
    10081 tcaatctaac tttcattcga aagtatttta atgtttgaac gaaacagaga ggcaactatg
    10141 attttcaata ttcagcgcta ctcgacccat gatggccccg gtatccgcac ggtcgtattt
    10201 cttaaaggct gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg
    10261 caggatctgc tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg cgctaaggcc
    10321 gcgccggaag tgattgagcg cgcgctgaat ggtttgctta ttcatcggga aaagttaacc
    10381 ccggagcatc tgacggcgtt aaccgactgc tgtccgacac aggcattaac cgtgtgtggt
    10441 gaagtgaaaa gcgttgagga gatcatgacg accgttctgc gcgataaacc gttttacgat
    10501 cgcagcggcg gcggtttaac gctttcgggt ggtgagccct ttatgcagcc ggaaatggcg
    10561 atggcgctac tgcaagccag ccacgaggca ggcattcata ctgcggtaga aacctgtctg
    10621 catgtgccgt ggaaatatat cgccccttct ctgccctata tcgatctgtt tcttgccgat
    10681 ttaaaacacg ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc cgccagagtg
    10741 ctggataacc tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg
    10801 attcagggct ttaatgccga cgaaacctct gtaaaagcta ttaccgattt tgccgccgac
    10861 gagctgcacg ttggcgaaat tcatttcctg ccctaccaca cgctaggcat caacaaatat
    10921 cacttactta atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc
    10981 gactttgccc agcagtatgc ctgccagaaa gggttaaccg cgaccttacg aggataacaa
    11041 ccatgaccac actgaaactg gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc
    11101 tggtgcatat tgtgaaaccg ccagtctgta ccgagcgcgc gcagcactat accgagatgt
    11161 atcaacaaca tctcgataag ccgatcccgg tacgtcgcgc gctggcactg gcgcatcacc
    11221 tggcgaatcg caccatctgg atcaaacacg atgagttgat cattggcaac caggcaagcg
    11281 aagttcgcgc cgcgccgatc ttcccggaat atactgtctc gtggatcgaa aaagagattg
    11341 atgatctggc agatcgtccc ggtgctggct ttgcggtgag cgaagagaac aaacgcgttc
    11401 tgcatgaagt gtgcccgtgg tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt
    11461 ttaccgatga gcaaaaaggt ctgctggcga ccggaatcat taaagcggaa ggcaatatga
    11521 cctccggcga tgcgcacctg gcggtgaatt tcccgctgct gctggaaaaa ggtcttgatg
    11581 gtctgcgcga gaaagtagcg gaacgtcgct cgcgcatcaa tctgacggta ctggaagatt
    11641 tgcacggcga acagttcctg aaagcgattg atatcgtgct ggtggcagtc agcgaacaca
    11701 ttgaacgttt tgcagccctg gcgcgtgaaa tggccgcgac cgaaacccgc gaaagccgtc
    11761 gcgatgaact gctggcgata gcagaaaact gcgatcttat cgcccaccag ccgccgcaga
    11821 ctttctggca ggcgctgcaa ctgtgttact tcatccaatt gatattgcag atcgaatcta
    11881 acggtcactc ggtatcgttt ggtcgtatgg accagtatct ctacccgtac tatcgccgcg
    11941 acgttgaact caaccagaca ctggatcgcg aacacgccat cgaattgctg catagctgct
    12001 ggctgaaact gctggaagtg aacaagatcc gctccggctc acactcaaaa gcctctgcgg
    12061 gaagtcctct gtatcagaac gtcaccattg gcggacaaac tctggttgat ggtcaaccaa
    12121 tggacgcggt gaatccactc tcttacgcta tcctcgaatc ctgcggtcgc ctgcgttcca
    12181 ctcagcctaa cctcagcgtg cgttaccatg caggaatgag taatgatttc ctcgacgcct
    12241 gcgtacaggt gatccgttgc ggcttcggga tgccggcgtt caacaacgac gaaatcgtga
    12301 tcccggaatt tattaaactc ggtattgaac cgcaggacgc ttacgactac gcagcgattg
    12361 gttgtatcga aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc atgagcttta
    12421 tcaacttcgc ccgcgtgatg ctggcggcgc tggaaggcgg gcgtgatgcc accagcggca
    12481 aagtgttcct gccacaagaa aaagcgttgt cggcaggcaa ctttaacaat ttcgatgaag
    12541 taatggacgc gtgggatacg caaatccgtt attacacgcg taaatcaatc gaaatcgaat
    12601 atgtcgtcga caccatgctg gaagagaacg tgcacgatat tctctgctcg gcgctggtgg
    12661 atgactgtat tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat gactgggttt
    12721 ctggcctgca ggttggcatt gccaacctcg gcaacagcct ggcggcagtg aagaaactgg
    12781 tatttgaaca gggcgcgatt ggtcagcaac agctggctgc cgcactggcg gatgacttcg
    12841 atggcctgac tcatgagcaa ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca
    12901 acgacgatga taccgtcgat acgctgctgg ctcgcgctta tcagacctat atcgacgaac
    12961 tgaaacagta ccataacccg cgctacggtc gtggtccggt tggcggcaac tattacgcgg
    13021 gtacctcgtc tatctccgct aacgtaccgt ttggcgcgca aactatggca acaccggacg
    13081 ggcgtaaagc ccacaccccg ctggcagaag gcgcaagccc ggcctccggt actgaccatc
    13141 ttggccctac tgcggtcatt ggctcagtgg gtaaactgcc tacggcagcg attctcggcg
    13201 gcgtgttgct caaccagaaa ctgaatccgg caacgctgga gaacgaatct gacaagcaga
    13261 aactgatgat cctgctgcgt accttctttg aagtgcataa aggctggcat attcagtaca
    13321 acatcgtttc ccgcgaaacg ctgctggagg cgaaaaaaca tccggatcag taccgcgatc
    13381 tggtagtgcg tgtcgcgggc tattccgcct tcttcaccgc gctctctcca gacgcacagg
    13441 acgatatcat cgcccgtact gaacatatgc tgtaatctcc tcggcccggc ggcgtcgtcg
    13501 ccgggctaaa tcattcacat catcaatttc atccttactt tcattcgaaa tataatttgt
    13561 gctctgcgtc acattgttat tacactgtta cggtttcatt ttcgagccag gagcacacct
    13621 atgagcgtaa aagttatcgt cacagacatg gacggtactt ttcttaacga cgccaaaacg
    13681 tacaaccaac cacgttttat ggcgcaatat caggaactga aaaagcgcgg cattaagttc
    13741 gttgtcgcca gcggtaatca gtattaccag cttatttcgt tctttcctga gctaaaggat
    13801 gagatctctt ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa gcaactgttc
    13861 cacggcgaac tgacccgaca tgaatcgcgg attgttattg gcgagttgct aaaagataag
    13921 caactcaatt ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa
    13981 gcatttgtcg cactgatggc aaaacactac catcgcctga aagctgtaaa agattatcag
    14041 gagattgacg acgtactgtt caagttttcg ctcaacctgc cagatgaaca aatcccgtta
    14101 gtgatcgaca aactgcacat agcgctcaat ggcattatga aacccgtcac cagtggtttt
    14161 ggctttatcg acctgattat tcccggtcta cataaagcaa acggtatttc gcggttactg
    14221 aaacgctggg atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg
    14281 gagatgctga aaatggcgcg ttattccttt gcgatgggca atgctgcgga aaacattaaa
    14341 caaatcgccc gttacgctac cgatgataat aatcatgaag gcgcgctgaa tgtgattcag
    14401 gcggtgctgg ataacacatc cccttttaac agctgacctc tctaccggag ccgtgcgctc
    14461 cggttttccc ttctttttac atcctgtaca tcacatcaaa tcattaaact tgcattaact
    14521 tatttataac ttatattgtc gcatgcaaat cagaatggtt tcgaatgaaa gttaatgaga
    14581 ggtggttatg gcttctactt ttaccagcga cacattgcct gccgatcaca aagcagctat
    14641 ccgtcagatg aagcacgcgc tgcgggcgca gcttggcgac gtccagcaga tctttaatca
    14701 gctaagcgat gacattgcca cgcgagtggc tgaaatcaac gcactcaaag cacagggcga
    14761 tgccgtctgg ccggtgctgt cttatgccga tatcaaagca ggtcatgtta ctgcagagca
    14821 gcgcgaacag attaaacgtc gcggttgtgc ggtgataaaa ggccatttcc cccgcgaaca
    14881 agcgctaggc tgggatcagt cgatgctgga ctatctggac cgcaaccgct ttgacgaggt
    14941 ctacaaaggc cccggcgata atttcttcgg gacgctcagc gcttcacgtc ccgagattta
    15001 ccccatctac tggtcgcagg cgcaaatgca ggcccgccag agtgaagaaa tggcgaatgc
    15061 gcagtcgttt ctcaatcgtc tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc
    15121 ggatgtgagc gtcatctacc ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa
    15181 aggtcttgga gcgcataccg actccggggc gctggaacgc tggctgcttc cagcgtatca
    15241 gcgcgttttc gccaacgtct ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc
    15301 acatcgtacg gaagttgaag agtacacggt ggacaacacc accaaatgtt ccgtgtttcg
    15361 gacattccaa ggctggacag cgctctctaa tatgctgcct ggtcaggggc tgctgcacgt
    15421 tgtgcccatt cctgaagcca tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc
    15481 ggaggatgaa ctgtgcggcg tagcgcccgg aagagtgttg ccggtatcag agcaatggca
    15541 tccactgtta attgaggcgt taaccagcat tccaaaactc gaggccggag actccgtctg
    15601 gtggcactgc gacgtcatcc attccgttgc ccccgttgaa aatcaacagg gttggggcaa
    15661 cgtgatgtac attcctgcgg caccaatgtg cgagaaaaat cttgcctacg cgcacaaggt
    15721 gaaggccgca ctggaaaaag gcgcatcgcc gggcgacttc ccgcgcgagg actatgaaac
    15781 aaactgggaa ggacgcttta cgcttgccga cctcaacatt cacggtaagc gagcgttggg
    15841 aatcgacagc tgagtgtcgt acagcccttc ccgttccacg gcggtgttcc cttttcctgg
    15901 gcgaacccgc cgcactgact cccgcaccgc caatcgtccg tgcagcagca gcgagcccac
    15961 cggagcatcc gggcgaatca gccgctgttg caacatttgc accgcttcat tgcctagctc
    16021 atcgcgtggc acatgcacag ccgtcagggg aacatcctcg attgccgcaa ggttaaagcc
    16081 gtcgatactc atcaccgaga catcctgtgg tacgcgcaga ccacgacgat gtagcgcatt
    16141 taccgttccc gccgccataa aatccccgcc gaccagaaac gccgtcggca acgtcttgcc
    16201 attggcggca tccagccacg ctccgacctt ttcttccgct tctttagcgc taaaactgct
    16261 gatattcagc aaatgacggt catggtgaaa ggccagatta tgctgacgcc atgattcttt
    16321 aatccctgca aaccgctgct ccatagtgta gcggcgcagg cacatcacat tcatcacctc
    16381 ccggtgaccc atttcaaaca gatagcgggc ggcgaacgcg ccaattaacc gatgatccgg
    16441 cgcaatcgcc gggaggcgca ttcgttcatc acggcagttg atcagcacac aaggcttagc
    16501 gagatcggcg gccagatcat ggatgtgcgg atcgtcaatc cccagcagga tcgctgcctg
    16561 cgtatctgct tcattcatcc gcgagagaaa gagcgcggga tcgctgtcaa actcctccag
    16621 cgcgcagtaa cgcagccgca cttcatgcgg cgataacgct ttgctgatgc tctggatcac
    16681 acggtaataa aagatatcag atcgttggtc aaaagcgcgt tgcggcgcga aaataatcag
    16741 gttgttcagc aacatacgac ctgctgccat gccatccatc acgcccaact cacgcgcgca
    16801 ggccagcacc tgttcgcgag cgcgtgcact ggtattcgct ttgccagcca gcacccgcga
    16861 aaccgtactc ggcgatagcc cggtacgtgc ggcgatatcg gcgattttca gcttcttgtc
    16921 cattttgtga tctgcctcta attcggaaat gacagaattt tcatgagcaa aaaagcccat
    16981 tctgtctggc attaaatcca tttcagtaat gtatatcagt cactcatgga aaaacgtgca
    17041 caaaacccgc tttctttcgc ttttagggtc tcttactgtg aattggtaga acaacttcca
    17101 tacaaaaata atggaaaaac gaacaataaa gactttattt atcaaaaagt taaaagtggt
    17161 gtaacaactc cattctgacg ttgccgcaca aaccctatac cattctgtgg agtgcatatg
    17221 agtcagggta tcaataacga tatgactgcc agtaaaccgc gccatatcgt caaaaatctg
    17281 cgttggtgga tactggtcct ttttttgctc ggtgttactg tcaactacat cacgcgtaac
    17341 tcgctgggta ttctcgcccc cgaactgaaa agcagcctag ggattaccac cgaacaatat
    17401 tcctggattg taggtgcttt ccagctcgcc tataccctat tccaaccgtt gtgcggatgg
    17461 ctgattgacg tcattggcct caaactgggc tttatgatct gcgcgggact gtgggcactg
    17521 gcctgtctga tgcatgcggg ggcgggaaac tgggtacatc tggcgctgtt gcgcttcttt
    17581 atgggcggtg cggaagccgc ggcaacccca gcaaacgcca aaaccatcgg tgaatggttt
    17641 ccaaaatcag aacgcccgat cgccgcaggc tgggcaggcg tcggtttttc catcggtgcc
    17701 atgctggccc caccgattat ctattttgcc cacgcttcat tcggctggca gggtgcattt
    17761 atgttcactg gcatcctggc gatgctgtgg gtggtgctgt ggtgggcgtt ctatcacagc
    17821 ccggataagc atcctaacct cagcaaagcc gagctggact ttattcgcca ggataacgaa
    17881 gcgcccccgg tcaagttacc ctttctgacc gcactgaaaa ccgtgggcaa aaataaacgc
    17941 ttttacggta tcgctatccc ggcctttatg gcagaaccgg cgtgggcggt gatgagtttc
    18001 tgggtgcctc tatacctgtc caaaacctat ggcatggacc tgaagcagat cgcattattc
    18061 gcctggttgc cgttcctcgc cgctgatctt ggcagtattg ccagcggcta tctgaccaaa
    18121 ctttacaccc gcctgtttgg gctgagtcgt gtgaactccg ttgtcgcatc atcggtcagt
    18181 ggcgcattct tgatggtttc gctggcggtg atggccttta ccaataaccc gtatatcgcc
    18241 atcgtcctta tctccatcgg cggcttcggt caccagatca tctcctgcat gctgagcgca
    18301 ctggtggtgg aatccttcga caaaggtcag atggccactg ttaacggcat gcgcggttct
    18361 gccgcgtgga tcgcaagttt cctgttctcc ctgattatcg gggttaccgc tgacaaaatc
    18421 ggctttaacc ccctgtttat cgccatgggc ttctttgacc tgattggcgc tttcttcctg
    18481 gtcaccttta ttgctgaacg tcgcgcaaaa cgcgcctgag agtggatgta aacatgaaaa
    18541 cgctgaaaaa ctggaccctg cacaaacagt ctgcccacca cgttgaactg ctggtcgatg
    18601 gccagcatat cctgtgcctg tacgtgctgg aagaaaatct gttccgcgtg ctgttaaaac
    18661 gcaaaggcga gctggcgcta gatcgcacct ggagcattgc tccggcgcag gatgtgccat
    18721 gggaagggcg cagccgcgaa gatctcagcg gctttagcct gcccgcgtgg acagtgatcc
    18781 agcacgacga caccgtggtt atcgccaccg agtcactgcg tgcaaccgtt caccagccgc
    18841 tgtggctgga gtggcactat cgtaacaacg aaggcgaatg gctgccgttg gtgaatgacc
    18901 gcccgaccag cgcttatctg ctgaatgccc acggcgatgg cgtggcacac tatcagagcc
    18961 gtcgtaaaga tgaacgcttt tatggcctcg gagataaagc cggcgatctg caacgcaacg
    19021 gtaaacgtta cgagatgcgt aacctcgacg cgatgggcta caacgccgtt agtactgacc
    19081 cgctgtacaa acacattcca ttcaccatca cccgccgcga tgacattagc ttcggcctgt
    19141 tttatgacaa cctcagcagc tgctggctgg atctgggcaa cgagatcgac aactaccaca
    19201 ccgcctatcg ccgctggcag gcggaagcgg gtgatatcga ctactacctg tttaccggcg
    19261 aacgcgtact tgatatcacc aaagccttcg tacgcctgac cggaaaaacc ctgttcggac
    19321 cgaaatggag cctgggctac agcggttcaa ctatgcacta caccgatgcg ccggacgcac
    19381 aaaatcagtt gatgaacttt atccgcctgt gtgaagaaca tgcaattccc tgcgattcgt
    19441 tccagctctc ttccggttat acctcaatta acggcaaacg ctatgtgttc aactggaact
    19501 acgacaaagt gccgcagccg aaagtgatga gccaggcatt tcacaatgct ggtttgcacc
    19561 tggccgccaa tatcaagccg tgcctgttgc aggatcaccc acgctataac gaagtggcag
    19621 aaagaggact gttcatccgt gattcagaaa cagatgaacc agaacgttcc agcttctggg
    19681 atgacgaagg ttctaacctc gactttacta atccgcagac catccagtgg tggcaggaag
    19741 gcgtgaccac acagcttctg gagatgggta tcgactccac ctggaacgac aacaacgaat
    19801 tcgaagtatg ggacggtgaa gcacgctgtc acggctttgg tcagacgatc gcgatcaaac
    19861 atattcgccc agtgatgcca ctgctgatga tgcgagcctc gatggaagcc cagcagcgtt
    19921 tcgctccgga aaaacgtccg tacttgatct cccgctccgg ctgcgcggga atgcagcgct
    19981 acgtgcagac ctggagtggc gataaccgca ctagctggga cactctgcgc tacaacaccc
    20041 gcatgggtct ggggatgagc ctctccgggc tgtataacct cggtcacgac gtcggcggtt
    20101 tctccggtga taagccggac ccggaactgt ttgtacgctg ggtacaaaac ggcgtgatgc
    20161 atccgcgctt tacgattcac tcatggaacg atgaccatac cgtaaacgaa ccgtggatgt
    20221 atccgggcgt cactccggcg atccgcagcg ctattgagtt gcgctatcgc ctgctgccct
    20281 acttttatac cctgatgtgg caggcatacg ccgatgatga accaatgcta cgtccgactt
    20341 tccttgacca cgagcacgac gttcagactt ttgaagagtg tgatgacttc ctgcttggtc
    20401 gcgatatttt ggttgccagc gtcgtagagc aaggcgagcg ccagcgccgt gtatggctgc
    20461 cagataacga aactggctgg tacgatttct acaacggcga atggttcagt ggcggtcagt
    20521 ggatcaccct cgacgcaccg ctggagaaac tgccgctgat ggttcgtgcc ggggctggtc
    20581 taccgctcag caaacgcatc acctatgtga gcgcagaaca agacgatacc cgcgaactga
    20641 aactgttccc gctgaaaggt gtgggcacaa cgtccggcct gctgtttgaa gacgatggcg
    20701 aaagctgggg ctaccaaact ggtaatgcgc tttgggtgga atgggaaatg gtttgcgatg
    20761 gggcgaccat caacctgaaa gtcaacgcgc gcggggatta tcgtcctgca tggagtgcac
    20821 tgaaagtgtc attaccattg gaagaaaaac gcacgctgct ggtcaatggt gttgaaggga
    20881 gtgaatggat gcgttaaatc attaccgggg ccgggaggcg actgcacctt acccggtcca
    20941 gccatcgaat cctttacctc agtatttcac gctgcaggaa ggcagcaaac ctgtaaaccc
    21001 cgggagctta aataagtaag tgaccggggt gaacaggtga agccaatgca tctgccgcat
    21061 gaaagacggc ggggaattaa gcgtcgatcc ctttactacg caggtaatct tcgtaattac
    21121 cgctaaagtc gatcgcgcgt tccggggtga tttccagaat gcgggtcgcc agggagctta
    21181 cgaactcacg gtcgtgtgaa acaaagatca gcgtgccctg atacagttcc agcgccatgt
    21241 tcagtgactc gatggattcc atatccaggt ggttggtcgg ttcgtccatg atcagaatgt
    21301 tcggcttctg catcattaac ttaccaaaca gcatccgccc tttttcccca ccggaaagca
    21361 ctttagctgg ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta
    21421 ccgcctgctc gtcatcgcct tcctgcttcc actgactcat ccattcgaac acggtcagat
    21481 cattttcaaa ctcatattcg tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc
    21541 attttacggt gccgctgtcc ggttgcagat cgcccaccag cgttttcagc agcgttgatt
    21601 taccgacgcc gttggtaccc agtaccgcaa gtttttcacc gacctccagc agcaggttga
    21661 gatttttaaa cagtggaccg ttatcaaacc ctttggtcag accttccact tccagcgcgt
    21721 tacggaacag tttcttatcc tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt
    21781 tcacctcttc cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt
    21841 tcgaggcgtt ggcgctaaag cggctaacga aagattgcaa ctcagcaatc tgcgctttct
    21901 tcttggcgtt atcggccagc agacgttcac gcgcctgggt cgccgccgtc atgtactcat
    21961 cgtagttacc cggataaacg cgcagctcgc cgtaatccag atccgccatg tgggtacaga
    22021 ccatgttaag gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca
    22081 gcacctgttc cagccagcga atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca
    22141 ggagaatatc cggatcagca aacagcgcct gcgccagaag cacacgcagt ttccagccag
    22201 gagcaacttc actcatcggg ccgtagtgct gttccactgg aattcccacg ccaagcaaca
    22261 gttcaccggc gcgagcttcc gcagagtaac cgtccatttc gccgtattta acttccagat
    22321 cggccacttt atagccgtct tcctcactca tttccggcaa agcatagatg cggtcacgct
    22381 cctgcttcac ttcccacaac tctttatgcc ccatgatcac cgtatccagc acagtgaact
    22441 cttcaaaggc aaactgatcc tgacgcagct taccaatgcg ctcgttggga tcgagggaaa
    22501 cgttacccaa agtcggctct aagtcgccgc cgaggatctt cataaaggtg gatttaccac
    22561 taccgttcgc gccaatcagg ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt
    22621 caaacaacgg cttactgccg aactgcatgg tgacgttact ggaaactaac acaggcgtat
    22681 cctgaaaaga gatatgacaa accgcgcatt atgccataag tctgaaagaa gatcctgctc
    22741 ctcgcttaag gtctacactc aagccaaagg caaaaaatgt gatttcgtac acatctgatt
    22801 tcactgtgag ctggaatgaa cttataatgc gcttccaata ctctaatatt ctcaacccaa
    22861 tggcctgcca ggcacaaaat ctcgcttaac atgaatatga aattgaaaac attattcgca
    22921 gcggccttcg ctgttgtcgg cttttgcagt accgcctctg cggtaactta tcctctgcca
    22981 accgacggga gtcgcctggt tggtcagaat caggtgatca ccattcctga aggtaacact
    23041 cagccgctgg agtattttgc cgcggagtac cagatggggc tttccaatat gatggaagcg
    23101 aacccgggtg tggatacctt cctgccgaaa ggcggtactg tactgaacat tccgcagcag
    23161 ctgatcctgc cggataccgt tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg
    23221 tattactatc cgaaagggac caacaccgtt atcgtgctgc cgatcggcat tggtcagtta
    23281 ggcaaagata cgcctatcaa ctggaccacc aaagttgagc gtaagaaagc aggcccgacc
    23341 tggacgccga ccgccaaaat gcacgcagag taccgcgctg cgggcgaacc gcttccggct
    23401 gtcgttccgg caggtccgga taacccgatg gggctgtatg cactctacat cggtcgcctg
    23461 tacgctatcc atggcaccaa cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt
    23521 gtgcgtctgc gtaacgaaga catcaaattc ctgttcgaga aagtaccggt cggtacccgc
    23581 gtacagttta ttgatgagcc ggtaaaagcg accaccgagc cagacggcag ccgttatatt
    23641 gaagtccata acccgctgtc taccaccgaa gcccagtttg aaggtcagga aattgtgcca
    23701 attaccctga cgaagagcgt gcaaacagtg accggtcagc cagatgttga ccaggttgtt
    23761 cttgatgaag cgattaaaaa ccgctccggg atgccggttc gtctgaatta atcttcaaaa
    23821 cttaaagcaa aaggcggact cataatccgc cttttttatt tgccagaccc tagttggccg
    23881 ggagtataac taacaaaaca tatcccacca acgccgccca caacagcatc ggtattgaat
    23941 agagatggaa acgccaccag atgcggcgat cgttcgccat acgtagcgca atcaaatttg
    24001 ccagcgatcc gggtaataac ccaaagccac ctacgtttac cgcccatacc agtaataaag
    24061 acggcggcac atagttcagc aacaatatgg tactcggcac gttactgatc acctgcgata
    24121 aaccgattgc cgttaaccat aacccgggtt cagatagatg actcacgtta cccaacacgc
    24181 cttgcaacgc tggaagctgg gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca
    24241 gcagcagcgt ccagtccaca ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg
    24301 ccacaatcac cagtccccac agctcttgtt tgaactccag cgccgtcaga aagacgatat
    24361 acagccccag acaactccac accagccgcg gtttccactc cggtgtttgc acccccgtat
    24421 ggtattgcag cgcctttcca gggaaacaac accagcacag gagcaggagc gtcagcatca
    24481 ttgcgccagc cagcggtgcc atttgggcaa taaatccggc aaacgaaaga ccagaacgtc
    24541 cccagataag aatattttgc gggttgccaa ttggcgtcag tagcgaacca gcgttgactg
    24601 ccagcgcctc aaaaataatc agccgattaa ccgggatctc acacagtctt tttagcgtga
    24661 tagtcagcgg aacaacaata aacagcgcga catcgttggt cagaaaggta gaaagcagcg
    24721 ccgccgccag caccataaac atcgccagcc gacgctccgt agcaaagcgg cgcaccattt
    24781 tgcgccccag cacatcaaaa taaccgctta actccacacc tttggtcagc agcatcaggc
    24841 cgcttaaggt gatgatggtg tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg
    24901 gcacgaaaaa gcttaatccg ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt
    24961 gcagcgtgcg taaaaaaggc aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa
    25021 agcgtcgagc gtctcttcac taacatggtg ctccatgcct tccgcgtcgc gacgggcgat
    25081 ttccggactg acgcccaaca ccaacaagaa attttcgact atctgatgtc gctcgcggct
    25141 ttcctgcgcc agcttctctc cttctgccgt taaaaacacg ccacgccagg ggatcatttc
    25201 aatcagcccc atggttgcca gccgcttaag cattttagcc accgtcggtt gcgaaactcc
    25261 cagacgagca gccatgtcca cctgacgagc ttcccccact tccctgatca agtcagaaat
    25321 cagctcaacg taatcatcaa taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc
    25381 ttcaacgtgc tcttccacgt tcactaactg cgtcactttt tttgctgttg gcgtacctgc
    25441 gcgacgactc attgtgcttc ctcattatgg tgacgcatac atagcgttct catttcgtgc
    25501 gcacattgta aaccagagtt gcgaaggtac aaaaaattaa cgttttagca atagctacat
    25561 aatatagcct gtgctatatc tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta
    25621 gcaagtcagg aggtcttatg aatgagttca agaagtgtat gcgcgtgttt agtcattctc
    25681 cctttaaagt acggttaatg ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc
    25741 agcaagataa accttccgat aaatagcggc gtcgcggtac gccgcttcac tcctgctttt
    25801 atgcaggcat aacgcgtttt ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc
    25861 ttcgcaaaag atttgttcgt cagtagttga cctgaacggc ggctcgctct atcttcttgc
    25921 agccctgcgt atattgcggc tcgcggatgc ggaccccttt cccctcttca cgcactcttg
    25981 caggtattga cccttgacgc cagggtaagc acatggcgtt tgttacgata gtggcatatg
    26041 aatttaaccc tcaaagaatc gcttgttacc cgtagccggg tatttagccc gtggactgcg
    26101 ttctactttt tacagtcgct attaattaac ctcggcttag gttacccctt cagtttgctc
    26161 tacaccgctg cgtttacggc tattttgctt ttgctatggc gaacattgcc tcgcgtacaa
    26221 aaagttctgg tcggtgtcag ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc
    26281 tacggcgcgc ctaatttcaa tacattgctg gcattgcact ccaccaatat ggaagagtcg
    26341 accgaaatcc tgacgatttt tccgtggtac agctacctgg tcggcttatt tatttttgcg
    26401 ctcggcgtaa tagcaatcag gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc
    26461 gacagcctgt gtctggtatt cagtgtggcg acattttttg ttgctcccgt gcaaaacctg
    26521 gcctggggtg gcgtatttaa actgaaagat actggctatc cggtatttcg ttttgctaag
    26581 gatgtcatcg tcaataataa cgaggtgatt gaagagcaag aacggatggc aaaactttcc
    26641 ggaatgaaag atacctggac ggtcactgcc gttaagccga agtatcagac ctatgtggtg
    26701 gtgatcggcg aaagcgcgcg tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat
    26761 accccgtttg ccagcagcgt taacggtttg atatttgctg actacattgc cgccagtggc
    26821 tccacgcaga aatcacttgg cttaacgctc aatcgcgtag tcgatggcaa accacagttt
    26881 caggataact ttgtcaccct ggcaaatcgc gcgggcttcc agacctggtg gttttccaac
    26941 cagggccaaa tcggcgaata cgatactgct atcgccagca tcgccaaacg tgcagatgaa
    27001 gtgtacttcc tgaaagaagg taattttgaa gcagataaaa acacgaaaga cgaagcgtta
    27061 ctggatatga ccgctcaagt gctggcgcaa gagcactcgc aaccgcagct gattgttctg
    27121 catctgatgg gctcgcatcc gcaggcctgc gacaggacac aaggcaaata cgaaaccttt
    27181 gtgcaatcga aagaaacgtc gtgctatctc tataccatga cgcaaacgga cgatttactg
    27241 cgcaagctgt acgatcagtt acgcaacagc ggcagcagct tctctctggt ttacttttct
    27301 gaccacggtc tggcctttaa agagcgcggc aaagacgtgc aataccttgc ccatgatgat
    27361 aaatatcagc aaaatttcca ggtgcctttt atggtcattt ccagcgacga taaagcgcat
    27421 cgggtgatta aagcccgccg ctcagccaat gacttcttag gctttttctc gcagtggacg
    27481 ggaattaaag cgaaggaaat aaatatcaaa tacccgttta tatctgagaa gaaagccggg
    27541 ccgatataca tcaccaactt ccagttacag aaggtggatt acaaccatct cggaaccgat
    27601 attttcgacc cgaaacctta aaacaaaaaa tccgccccga gaggcggatt ttttatatca
    27661 ccaaagtgat tagaagcggt aaccaacacc ggcaatccag gtgcctacgt caacgctacg
    27721 aatacggctc tgctcgtaag agaagtccag agcaacgttt tccatcgggt tgaactgcag
    27781 accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt
    27841 ggtctggaat ttaccataac ccacccccac tacaccgtag atgcttgccc agtcgttaat
    27901 gcggtaagcc ggaccagcag tgatgccgta gtactggttt ttgttgtagt caccagagct
    27961 tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc
    28021 ttcttcatag cggtatttca ggttgaaacc gcccattttg ttcatttggc cctgagcgtc
    28081 gctctgtgcg taaccgccag ttacggtaga agtcgcagct acggaagtac ctgcggtgaa
    28141 agccagaact gcggccagtg ctgaaagaca tgcaattttt ttcataacca cctcaaatgt
    28201 gattcaaata agttctaagt tttaaatata tcaaaaatta a
//
LOCUS       DAZPBM010000038        28104 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPBM010000038 DAZPBM010000000
VERSION     DAZPBM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.038"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUZQ_001514"
     CDS             complement(<1..215)
                     /locus_tag="ACHUZQ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1025241.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUZQ_001515"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUZQ_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1025242.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUZQ_001516"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUZQ_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1025243.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUZQ_001517"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUZQ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1025244.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUZQ_001518"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUZQ_001518"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1025245.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUZQ_001519"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUZQ_001519"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1025246.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUZQ_001520"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUZQ_001520"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1025247.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHUZQ_001521"
     CDS             complement(4604..5326)
                     /locus_tag="ACHUZQ_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1025248.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUZQ_001522"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUZQ_001522"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1025249.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHUZQ_001523"
     CDS             6526..6744
                     /locus_tag="ACHUZQ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1025250.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUZQ_001524"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUZQ_001524"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1025251.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHUZQ_001525"
     CDS             complement(7478..7678)
                     /locus_tag="ACHUZQ_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1025252.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUZQ_001526"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUZQ_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR1025253.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUZQ_001527"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUZQ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1025254.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHUZQ_001528"
     CDS             10175..12088
                     /locus_tag="ACHUZQ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1025255.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHUZQ_001529"
     CDS             12088..13110
                     /locus_tag="ACHUZQ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1025256.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHUZQ_001530"
     CDS             13104..13322
                     /locus_tag="ACHUZQ_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1025257.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHUZQ_001531"
     CDS             13376..14245
                     /locus_tag="ACHUZQ_001531"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1025258.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHUZQ_001532"
     CDS             complement(14300..14704)
                     /locus_tag="ACHUZQ_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1025259.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHUZQ_001533"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHUZQ_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1025260.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHUZQ_001534"
     CDS             15689..17779
                     /locus_tag="ACHUZQ_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1025261.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUZQ_001535"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUZQ_001535"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1025262.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUZQ_001536"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUZQ_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1025263.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHUZQ_001537"
     CDS             complement(19747..20349)
                     /locus_tag="ACHUZQ_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1025264.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHUZQ_001538"
     CDS             complement(20339..20506)
                     /locus_tag="ACHUZQ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1025265.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUZQ_001539"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUZQ_001539"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1025266.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUZQ_001540"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUZQ_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR1025267.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUZQ_001541"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUZQ_001541"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1025268.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUZQ_001542"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUZQ_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1025269.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUZQ_001543"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUZQ_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1025270.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUZQ_001544"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUZQ_001544"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1025271.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6787 a         7558 c         7114 g         6645 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagta gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agag
//
LOCUS       DAZPBM010000039        28040 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPBM010000039 DAZPBM010000000
VERSION     DAZPBM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.039"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHUZQ_001545"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHUZQ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR1025272.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHUZQ_001546"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHUZQ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHUZQ_001547"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHUZQ_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHUZQ_001548"
     CDS             complement(1702..3318)
                     /locus_tag="ACHUZQ_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1025273.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHUZQ_001549"
     CDS             3614..4330
                     /locus_tag="ACHUZQ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1025274.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHUZQ_001550"
     CDS             complement(4327..5988)
                     /locus_tag="ACHUZQ_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1025275.1"
                     /translation="MNDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHUZQ_001551"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHUZQ_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1025276.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHUZQ_001552"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHUZQ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1025277.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHUZQ_001553"
     CDS             7443..7775
                     /locus_tag="ACHUZQ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1025278.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHUZQ_001554"
     CDS             complement(7777..8991)
                     /locus_tag="ACHUZQ_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1025279.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHUZQ_001555"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHUZQ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1025280.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHUZQ_001556"
     CDS             complement(10153..11490)
                     /locus_tag="ACHUZQ_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1025281.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHUZQ_001557"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHUZQ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHUZQ_001558"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHUZQ_001558"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1025282.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHUZQ_001559"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHUZQ_001559"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1025283.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHUZQ_001560"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHUZQ_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1025284.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHUZQ_001561"
     CDS             15153..15809
                     /locus_tag="ACHUZQ_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025285.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHUZQ_001562"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHUZQ_001562"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1025286.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHUZQ_001563"
     CDS             complement(16782..17180)
                     /locus_tag="ACHUZQ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1025287.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHUZQ_001564"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHUZQ_001564"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1025288.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHUZQ_001565"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHUZQ_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1025289.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHUZQ_001566"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHUZQ_001566"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1025290.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHUZQ_001567"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHUZQ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1025291.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHUZQ_001568"
     CDS             23741..23821
                     /locus_tag="ACHUZQ_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025292.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHUZQ_001569"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHUZQ_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1025293.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHUZQ_001570"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHUZQ_001570"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1025294.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHUZQ_001571"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHUZQ_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1025295.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHUZQ_001572"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHUZQ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1025296.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHUZQ_001573"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHUZQ_001573"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1025297.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6759 a         7565 c         7158 g         6558 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcattcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca actgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa
//
LOCUS       DAZPBM010000040        27470 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPBM010000040 DAZPBM010000000
VERSION     DAZPBM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.040"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHUZQ_001574"
     CDS             complement(<1..81)
                     /locus_tag="ACHUZQ_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1025298.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHUZQ_001575"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHUZQ_001575"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR1025299.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHUZQ_001576"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHUZQ_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR1025300.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHUZQ_001577"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHUZQ_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1025301.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHUZQ_001578"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHUZQ_001578"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1025302.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHUZQ_001579"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHUZQ_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1025303.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHUZQ_001580"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHUZQ_001580"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1025304.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHUZQ_001581"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHUZQ_001581"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1025305.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHUZQ_001582"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHUZQ_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHUZQ_001583"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHUZQ_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1025306.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHUZQ_001584"
     CDS             complement(12387..12626)
                     /locus_tag="ACHUZQ_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1025307.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHUZQ_001585"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHUZQ_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1025308.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHUZQ_001586"
     CDS             13003..13290
                     /locus_tag="ACHUZQ_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1025309.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13960..14103
                     /locus_tag="ACHUZQ_001587"
     CDS             13960..14103
                     /locus_tag="ACHUZQ_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1025310.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14116..14325
                     /gene="cspE"
                     /locus_tag="ACHUZQ_001588"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14116..14325
                     /gene="cspE"
                     /locus_tag="ACHUZQ_001588"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1025311.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHUZQ_001589"
     CDS             14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHUZQ_001589"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1025312.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHUZQ_001590"
     CDS             complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHUZQ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1025313.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16292..16750)
                     /locus_tag="ACHUZQ_001591"
     CDS             complement(16292..16750)
                     /locus_tag="ACHUZQ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1025314.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="ACHUZQ_001592"
     CDS             complement(16805..17656)
                     /gene="manZ"
                     /locus_tag="ACHUZQ_001592"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1025315.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17669..18469)
                     /gene="manY"
                     /locus_tag="ACHUZQ_001593"
     CDS             complement(17669..18469)
                     /gene="manY"
                     /locus_tag="ACHUZQ_001593"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1025316.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18532..19503)
                     /gene="manX"
                     /locus_tag="ACHUZQ_001594"
     CDS             complement(18532..19503)
                     /gene="manX"
                     /locus_tag="ACHUZQ_001594"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1025317.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            19820..19978
                     /gene="yoaL"
                     /locus_tag="ACHUZQ_001595"
     CDS             19820..19978
                     /gene="yoaL"
                     /locus_tag="ACHUZQ_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1025318.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            19966..21522
                     /gene="yoaE"
                     /locus_tag="ACHUZQ_001596"
     CDS             19966..21522
                     /gene="yoaE"
                     /locus_tag="ACHUZQ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1025319.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(21526..23124)
                     /locus_tag="ACHUZQ_001597"
     CDS             complement(21526..23124)
                     /locus_tag="ACHUZQ_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1025320.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVQFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="ACHUZQ_001598"
     CDS             complement(23255..24619)
                     /gene="sdaA"
                     /locus_tag="ACHUZQ_001598"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1025321.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24803..25381)
                     /locus_tag="ACHUZQ_001599"
     CDS             complement(24803..25381)
                     /locus_tag="ACHUZQ_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1025322.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="ACHUZQ_001600"
     CDS             complement(25385..26746)
                     /gene="pabB"
                     /locus_tag="ACHUZQ_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1025323.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26820..26999
                     /locus_tag="ACHUZQ_001601"
     CDS             26820..26999
                     /locus_tag="ACHUZQ_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1025324.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27119..27418)
                     /locus_tag="ACHUZQ_001602"
     CDS             complement(27119..27418)
                     /locus_tag="ACHUZQ_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1025325.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
BASE COUNT         7067 a         6771 c         6941 g         6691 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc
    13561 ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat tgatagccgt
    13621 taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat
    13681 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
    13741 gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca
    13801 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
    13861 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
    13921 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
    13981 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
    14041 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
    14101 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
    14161 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct
    14221 atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
    14281 gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
    14341 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
    14401 atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
    14461 tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag
    14521 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
    14581 aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga tccgggcgac
    14641 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
    14701 cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg
    14761 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc
    14821 accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg
    14881 caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac
    14941 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc
    15001 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
    15061 atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta
    15121 cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
    15181 ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac actggcagca
    15241 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
    15301 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga
    15361 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt
    15421 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
    15481 aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
    15541 agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa
    15601 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc
    15661 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg
    15721 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
    15781 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
    15841 agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca
    15901 tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca
    15961 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc
    16021 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
    16081 ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt
    16141 atttcattgc tttattgata acgattttca tttggattta tatatcaatg aaacgttaaa
    16201 ataaataaca aataagggaa gaggtaatat agcctaagct atgtctgatt attaaaaata
    16261 cactaaacat agctttggct aaattcataa cttattgagt tgaaacgaga agcttataaa
    16321 ttttttccag atcgtcgata tttcgaacgc ggattaacag ccgacgctgt tctaattgca
    16381 tcaccaacac gccatcttcc gacaagttca tcgctttgat tcggctatat tcaatccaga
    16441 cattggcgaa gaaaaaacct ttttgtttaa agatgatctt cggaacgcgg atccagaata
    16501 tataaaaacc catcagagcc agtgcgctta ataaccatgt cgttattaac gcaccatgat
    16561 tcgtgacgtt gttatagata agaatgacaa tcaatccgac gaagataacg ctatcgatgc
    16621 gaccacgccg gagcaaagga attgccagca gggtggggcc gttacggcgg ggcatgatga
    16681 actgatcgta gatcgcgaag gccaggagtg cggcgatgaa aagaatcagt accaggtccg
    16741 tgattgtcat taatcctcca gataaaaaaa cggggccaaa aggccccggt agtgtacaac
    16801 agtcttacag tcccagcagg ccgcaagcgt aaccagcgat accgatgacg aagaagccaa
    16861 cgatgatcca cagcgggtta acttttttgc gcagtagcca catacaagca aaggtcagca
    16921 gcagtggtac caggcctggc attaactggt ccagaatagt ctggacagta gtaacgtgtt
    16981 ctttgcccgt ctggtcagta atgcgagaga caaccagcgg gatgttgaca tgtgtccact
    17041 tgttaaccaa tgcccccatg acaaacaggc cgaggataga cgccccttcc gtcagttttt
    17101 gcaggaagcc accacccata tctttaacga tatcgatacc tttggagtaa ccatacgcta
    17161 cgccgtagta acgggttgcc agacgcacca ggttaaacag gatgaagaac agcagcggac
    17221 ctaacaggct gccgctcatc gcgataccgg cacccagtgc tgcaaatacc ggacgtacgg
    17281 ttccccagaa gatcgggtcg cctacaccag ccagcggccc catcaaaccg actttgatac
    17341 cgttgatagc accgtcgtcg atctctgcgc cattagcacg ctgttcttcc agcgccaggg
    17401 ttacgccgag aatcggcgca gccacgaacg gctgggtgtt aaagaactcc aggtgacggc
    17461 gaatagcttg tttacgagct tcgttgttct cagggtagag gcgacgaatt gccggtacca
    17521 tagagaagca gaaacccagt gcctgcatac gttcgaagtt ccatgaaccc tggaagaggt
    17581 tagaacgcag gaagacgcca cgaatatcac tttgagtgag ttttttctcg gtggtagttt
    17641 gagttgtatc aaccatttcg ctcacctgtt agtccagttc gttatcgaga tcgttgttac
    17701 cagctgcctg agcaggcgca ccggctacgc ggttgtattt cgggctaagt tggatgtaga
    17761 gcactgccat aacagtacca atcacaccca gagcaaccag gttaaagttg gtgaatgctg
    17821 cggttacgaa gccgaggtag aagaacggca tcaggtagcc agcacgcatc atgttgataa
    17881 ccatcgcata accaaccacc acgatcatgc caccggcgat attcagacca ttggtcacca
    17941 cttccggaat cgcattcagc atgttctgta cttcgctggt accaacagac agcgcaacga
    18001 tgacggccgg aatagccaca cgcattgctt gcaggaacag agaagaaacg tggatccagg
    18061 aaatcgctgt caggttgccg ttatcagcag ccttatccgc agcgtgctgg aaagcaacgg
    18121 taatagtacg aacgatgatg gtcagtacct ggcccgcagc ggccagaggg attgccagtg
    18181 cgatacctgc accaatgctc tgatgacctg cgataaccag aatggtagaa atgatagaag
    18241 ccagagcggc gtcaggcgca actgcagcac cgatgttcat ccagcccagc gcgatcattt
    18301 ccagcgtacc accgataata ataccggttt tcatatcccc aagaacgata cccaccaggg
    18361 tacacgcgat tagcggacgg tgaaactgaa attcatcgag gattgatccc atacctgcga
    18421 tacaggctac gataaatacc agcacaattt gaagagtggt aatctccatt gtacttctcc
    18481 tgtttacgta agacttaagt gtgaaaactg agtgataatc aacacaatac gttacttatc
    18541 gattttgctg atcagatcca tcattttcag tttcggatcg gtggaaacct tacggacttc
    18601 cagctcaata ccgcgcgcat tcagtttctt gaacgcctca atatcttttt catcaaccga
    18661 aaccgcgtta ttcacctggg ttttaccctg acggaatgcc ataccaccga cgttaacaga
    18721 ggtgattttc acgccgcctt caacgagacg ctctacatct gttgggttgg taaataacag
    18781 cattacgcgt tcgccagcat atttcgggtt gttgtagacg cgaatcattt tggcaacatc
    18841 aactacgtgt gctgttacgc ccggaggtgc aacctgggtg agcagtgtct tacgaacggt
    18901 atccgcagcc acttcatcac taacaacaat aatacgggag acattggttt ctttggtcca
    18961 gcgggtggcg acctgaccgt gaatcagacg gtcgtcgata cgcgcaaggc caataaccat
    19021 gtagtcgttt ggccccattg gttttgccgg agttggagcc gcttttggtg ctgcggcagc
    19081 gggtgctggc gcggcttttt caaccggttt ggctttcagt gctttcacgc cttcacggcc
    19141 tgtttctact gccagcgcaa ccagttcatc aaagcttggg tcatcatcac gagccattaa
    19201 cgtttccacg agcattggaa tgttaacgcc tgcaatgact tcataatgct ctttgtcgac
    19261 gacaatgcgg ctggcagcat tgaacgggct gcctccccat gtatcaacga gaaacagcac
    19321 gcctttagtg gtgtcgagtt ttgccaactg agcgttgtac ttttcaatca gcgtttcggc
    19381 attttcacct ggaacgaaat cgatccagcc gacgttttcc tgctcgccta acagcatttc
    19441 tgccgtttta agcaactgct ctgcagccca accatgtgtg cctataacaa tagcaatggt
    19501 cacttgctac ctcctttatt atcgttaaca cctcaacgtg ccagatgtat ttttgaatcg
    19561 tcgtccacaa tcgaatcgat tcagataagg gctacaacca aaaacccctg cgtttcgcga
    19621 tttattttag atatcgaaaa aattatttta tgtgatgaag atccgtaatt taactttcga
    19681 ttagcagaaa tttcgaaagg taaaatatcc ttgtcacatt cgtttgcaaa ggaaggtaaa
    19741 tctttgccaa atcagaggcg tctctgatat gtttaactcc cgtttaacaa ccatggagta
    19801 tagggcagta gcccgcagta tggatcgtca ccgacgtcat ttcagcatca ggccttttaa
    19861 cgcctgcctt tctggcactc tatgccgcac ctttcgtttg cattttgtcg ttacgcctgc
    19921 attatttctg gcgtcgaata gctattcctt aagcaggagc ttgtcatgga attcttaatg
    19981 gacccctcaa tttgggcggg gctactcacg cttgttgttc tcgaaattgt gctgggtatc
    20041 gataacctgg tcttcatcgc cattcttgct gacaaactgc cgccaaaaca acgcgataaa
    20101 gcgcgtttgc tggggttatc actggcgctg attatgcgtc tggggctgct gtcgctgatt
    20161 tcatggatgg tcacgctgac caaaccgcta tttaccgtca tggatttctc cttctccgga
    20221 cgcgacctga ttatgttgtt cggggggata ttcttgctgt tcaaagcaac aaccgaactg
    20281 catgaacggc tggaaaaccg cgatcatgat tccggccacg gtaaaggcta cgccagtttc
    20341 tgggtggtcg tcacacagat tgtcatcctt gacgccgtct tctcgttgga tgcggtaatt
    20401 actgcagtag ggatggttaa ccatctgccg gtgatgatgg cggcggtagt gattgcgatg
    20461 gcggttatgt tgctggcatc caaaccgctg acgcgattcg ttaaccagca ccccacggtg
    20521 gtggtgctct gtctgagctt cctgttaatg attggtctga gtctggtggc agaaggtttc
    20581 ggtttccaca ttccgaaagg ttacctgtat gccgcgattg gcttctcgat catcatcgaa
    20641 gtgtttaacc agattgcgcg tcgcaacttt attcgccacc agtcgacttt gccgctgcga
    20701 gcgcgtactg ccgatgccat cctgcgtttg atgggcggga aacgtcaggc caatgttcag
    20761 cacgatgccg ataacccgat gccgatgccg atcccggaag gtgcatttgc cgaagaagaa
    20821 cgttacatga ttaacggcgt actgacgctt gcgtcgcgtt ctctgcgcgg gatcatgacg
    20881 ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat ccgcgagcaa
    20941 ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga tgaaatcatc
    21001 ggtattgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga tgtggcggcg
    21061 attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat caacctgttg
    21121 ggcgtgctgc gtcgtgctcg cgggagcttt gttatcgtga ccaacgagtt tggtgtggta
    21181 caaggtctgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt cccggacgct
    21241 gacgaaacgc cggaaatcat tactgatggt gacggctggc tggtaaaagg cggtacagat
    21301 ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga tgatatcgcg
    21361 acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg cgatgtgatt
    21421 gatgtagggc cactgcatat caccatcatt gaagccaatg attatcgtgt tgatctggtt
    21481 cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcattaac gtaacggcat
    21541 aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg ccagccattt
    21601 gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa tatgcacgcc
    21661 atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca ccagttcaat
    21721 attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa ccgcgtcaga
    21781 acctatagct gcggtaaatg acttatcaat tttcagcatg tcaggacgta atgtttcaag
    21841 ccaggaaaat gaactgttgc cggtgccgaa gtcatcaatc gccagtttga cgtttttacg
    21901 atgtagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt cggtgatttc
    21961 gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat cttttatcag
    22021 cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact gactactcat
    22081 cgggaaaaca tggcgctgac gaatggtttc tgccatcaca tagcgggtca gtggcacaat
    22141 taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc cctgacgcgg
    22201 attgttccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg cattaagcaa
    22261 aggctgacag aacaattcga actcatgttg cgccaggccc agattgattt cgcgggaaaa
    22321 gctcatccgg taagcggtcg ccagccaggc gatgtagccc accagtaaac ttagcagcac
    22381 cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc ccggaccatt
    22441 aacgttaatg gtaaaaggaa agcgttgcga agaaacctga tagttttcat tgtcttcagg
    22501 ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt ccaccgtcag
    22561 gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca gtaagtcgat
    22621 gttaatcatc tccatgaccc cagcattgct actgcccgct gcaggcgtcc attgaatcaa
    22681 aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg gttgcggtgc
    22741 aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa tgctggagca
    22801 atagagtgtt ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt ttgccgcctg
    22861 tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa gcaagacttc
    22921 gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt gattggcgaa
    22981 ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aattgcacgc tcagggtcga
    23041 agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc gataggtttt
    23101 aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg acaaacgcct
    23161 gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg ggtataaatt
    23221 cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact ttgattgcca
    23281 gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccagtt tcgtacatcg
    23341 tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga gccatccgtg
    23401 cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc acctgaacct
    23461 gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt tccgccgcca
    23521 cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca gccattgaac
    23581 aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag gcgttcattt
    23641 tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg tccgggctga
    23701 ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg caggcaccgt
    23761 tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact gccagcgcaa
    23821 acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg ctggaaacta
    23881 acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc agtacacctt
    23941 cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc gcgaaatact
    24001 cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga ccagacagcg
    24061 aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac ggatacggca
    24121 cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata aaaccgccgc
    24181 cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg tggatttgca
    24241 taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg tcgcgcggga
    24301 aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctcttct acgtcgcgaa
    24361 taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca agacccataa
    24421 taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca taaacgtcca
    24481 cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg acgaactgtt
    24541 tacctgcctt cataggccct acggtatggg aagatgaggg accaatcccc accttaaaca
    24601 tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc cagtaacgaa
    24661 ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat tcacatgaat
    24721 taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa gtagtgtttt
    24781 gtagatgtaa agataagtat agtcagggtt tcacaccaat ttgcagcgcc agctcacgaa
    24841 ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggacagc catacccgat
    24901 gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt aatgcctggg
    24961 cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac ggcagatcgg
    25021 gcgggataat gccgaccact ggggttacct gatagccagt tacgctatcg acgggcggca
    25081 gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca gcttcgcgca
    25141 gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg aatgccactt
    25201 gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc ggttgcggtc
    25261 gacggacgat ggggattaac acggcagcct gacgatgatt tagggtttcc cggttaattt
    25321 gcgggcgcaa aagttgaaag cgcgataaaa aatcatcaag catcaggcta cggtattcca
    25381 cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt tcctgatatt
    25441 ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat atttgtccgt
    25501 taatggcagt cagagtgcgg atagtaatgc tggtatccat gttgccgcaa aagctcaaat
    25561 agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg ataatttcca
    25621 tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg cgcagcagat
    25681 cgctggcgtg taactgttct ggtagttgtg ccgttatggt gctgaccaga tgatgcacgg
    25741 cagggaaggg ttccaccacg aacagctctg gtacttttac cgaacctgct acggcaacac
    25801 gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga tctttcgctg
    25861 agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg cgtggtagcg
    25921 tgcctttaat agggcgggtc tggatttcac tattatcaca aagaataaac cgctctggcg
    25981 aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc gcgcggttgg
    26041 cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca tgaaaacgct
    26101 gggcgagatt tacctgatag caatcaccgc tgtgcagata ttcctgtacc tggcgaaatt
    26161 tttcgccgta ctgctcgcgg gtcatattgg attgccagtc ggaagtaagc gtgaaatctt
    26221 cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca tcattatgac
    26281 tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg tagataccca
    26341 ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac tcaaaacggc
    26401 ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa tcttcgttat
    26461 gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc gggtcatcag
    26521 tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa gtggttaaag
    26581 tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg gcatagccgg
    26641 agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat tcagcggcgt
    26701 cctgacgcca gggtaaagta atcacagcgg gagataacgt cttcatcagt cctgactcta
    26761 ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag gagttaacaa
    26821 tgtttgcagg tttaccttca ctcacccatg aacagcagca aaaagctgtc gagcggatcc
    26881 aggaactgat ggcacaaggg atgagcagcg gccaggcaat cgcgttggtg gcagaagagt
    26941 tgcgtgccaa ccattccggc gagcgaatcg tggcgcgttt tgaggatgaa gacgagtaac
    27001 acactaatcc gtattgattt cgttatacaa gccgatgatt atttgattgt gagaaaaagg
    27061 acgcttttta tatgtgatta aaagcgtcct gatccattta tgctgatgta aattttactc
    27121 agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga tttttaatca
    27181 cagagcgttc acctgactcc attttttttg cccatccaac cattttttcc gtaaacccag
    27241 ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgca ggtactccca
    27301 gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa cttggtgttg
    27361 gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca gcaggcatat
    27421 tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
//
LOCUS       DAZPBM010000041        27108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPBM010000041 DAZPBM010000000
VERSION     DAZPBM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.041"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..924
                     /locus_tag="ACHUZQ_001603"
     CDS             <1..924
                     /locus_tag="ACHUZQ_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1025326.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHUZQ_001604"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHUZQ_001604"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1025327.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHUZQ_001605"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHUZQ_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1025328.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="ACHUZQ_001606"
     CDS             3885..4280
                     /locus_tag="ACHUZQ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1025329.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="ACHUZQ_001607"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="ACHUZQ_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1025330.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="dolP"
                     /locus_tag="ACHUZQ_001608"
     CDS             4900..5475
                     /gene="dolP"
                     /locus_tag="ACHUZQ_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1025331.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="ACHUZQ_001609"
     CDS             complement(5589..6629)
                     /locus_tag="ACHUZQ_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1025332.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="ACHUZQ_001610"
     CDS             complement(6702..7337)
                     /locus_tag="ACHUZQ_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1025333.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHUZQ_001611"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHUZQ_001611"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1025334.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="ACHUZQ_001612"
     CDS             complement(7963..8406)
                     /locus_tag="ACHUZQ_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1025335.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHUZQ_001613"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHUZQ_001613"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1025336.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="ACHUZQ_001614"
     CDS             complement(8746..9249)
                     /locus_tag="ACHUZQ_001614"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1025337.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="ACHUZQ_001615"
     CDS             complement(9243..9767)
                     /locus_tag="ACHUZQ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1025338.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="ACHUZQ_001616"
     CDS             9976..10971
                     /locus_tag="ACHUZQ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1025339.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="ACHUZQ_001617"
     CDS             10980..11858
                     /locus_tag="ACHUZQ_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1025340.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="ACHUZQ_001618"
     CDS             12064..13071
                     /locus_tag="ACHUZQ_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR1025341.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHUZQ_001619"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHUZQ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1025342.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="ACHUZQ_001620"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="ACHUZQ_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1025343.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="ACHUZQ_001621"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="ACHUZQ_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1025344.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="ACHUZQ_001622"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="ACHUZQ_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1025345.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="ACHUZQ_001623"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="ACHUZQ_001623"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1025346.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="ACHUZQ_001624"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="ACHUZQ_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1025347.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="ACHUZQ_001625"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="ACHUZQ_001625"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1025348.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="ACHUZQ_001626"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="ACHUZQ_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1025349.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="ACHUZQ_001627"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="ACHUZQ_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1025350.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="ACHUZQ_001628"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="ACHUZQ_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1025351.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="ACHUZQ_001629"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="ACHUZQ_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1025352.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26829..26905)
                     /locus_tag="ACHUZQ_001630"
     tRNA            complement(26829..26905)
                     /locus_tag="ACHUZQ_001630"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26869..26871),aa:Met,seq:cat)
BASE COUNT         6424 a         7518 c         6809 g         6357 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcag tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga taagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct caatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatggc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaagctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26881 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    26941 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    27001 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    27061 ttcactttca tttgttgaat acttttgcct tctcctgctc ccccttaa
//
LOCUS       DAZPBM010000042        26891 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPBM010000042 DAZPBM010000000
VERSION     DAZPBM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.042"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHUZQ_001631"
     CDS             <1..807
                     /locus_tag="ACHUZQ_001631"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1025353.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="ACHUZQ_001632"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="ACHUZQ_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1025354.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="ACHUZQ_001633"
     CDS             2629..3948
                     /locus_tag="ACHUZQ_001633"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1025355.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4320..5702
                     /gene="dosC"
                     /locus_tag="ACHUZQ_001634"
     CDS             4320..5702
                     /gene="dosC"
                     /locus_tag="ACHUZQ_001634"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR1025356.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5727..8126
                     /gene="dosP"
                     /locus_tag="ACHUZQ_001635"
                     /gene_synonym="pdeO"
     CDS             5727..8126
                     /gene="dosP"
                     /locus_tag="ACHUZQ_001635"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR1025357.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8385..8966
                     /gene="ddpX"
                     /locus_tag="ACHUZQ_001636"
     CDS             8385..8966
                     /gene="ddpX"
                     /locus_tag="ACHUZQ_001636"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR1025358.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8980..10530
                     /locus_tag="ACHUZQ_001637"
     CDS             8980..10530
                     /locus_tag="ACHUZQ_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1025359.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10532..11554
                     /locus_tag="ACHUZQ_001638"
     CDS             10532..11554
                     /locus_tag="ACHUZQ_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1025360.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11551..12447
                     /gene="ddpC"
                     /locus_tag="ACHUZQ_001639"
     CDS             11551..12447
                     /gene="ddpC"
                     /locus_tag="ACHUZQ_001639"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1025361.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12444..13430
                     /locus_tag="ACHUZQ_001640"
     CDS             12444..13430
                     /locus_tag="ACHUZQ_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1025362.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13459..14355
                     /locus_tag="ACHUZQ_001641"
     CDS             13459..14355
                     /locus_tag="ACHUZQ_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR1025363.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="ACHUZQ_001642"
     CDS             complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="ACHUZQ_001642"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1025364.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15187..15402
                     /gene="bdm"
                     /locus_tag="ACHUZQ_001643"
     CDS             15187..15402
                     /gene="bdm"
                     /locus_tag="ACHUZQ_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR1025365.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15504..15641
                     /gene="sra"
                     /locus_tag="ACHUZQ_001644"
     CDS             15504..15641
                     /gene="sra"
                     /locus_tag="ACHUZQ_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR1025366.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15798..17495
                     /gene="maeA"
                     /locus_tag="ACHUZQ_001645"
     CDS             15798..17495
                     /gene="maeA"
                     /locus_tag="ACHUZQ_001645"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1025367.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17629..18631
                     /gene="adhP"
                     /locus_tag="ACHUZQ_001646"
                     /pseudo
     CDS             17629..18631
                     /gene="adhP"
                     /locus_tag="ACHUZQ_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18777..19061
                     /locus_tag="ACHUZQ_001647"
     CDS             18777..19061
                     /locus_tag="ACHUZQ_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1025368.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="ACHUZQ_001648"
     CDS             complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="ACHUZQ_001648"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1025369.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="ACHUZQ_001649"
     CDS             complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="ACHUZQ_001649"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1025370.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="ACHUZQ_001650"
     CDS             complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="ACHUZQ_001650"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23292..23294),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1025371.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24111..24992
                     /gene="yddG"
                     /locus_tag="ACHUZQ_001651"
     CDS             24111..24992
                     /gene="yddG"
                     /locus_tag="ACHUZQ_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1025372.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            25251..26351
                     /gene="ompC"
                     /locus_tag="ACHUZQ_001652"
     CDS             25251..26351
                     /gene="ompC"
                     /locus_tag="ACHUZQ_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1025373.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            26515..>26891
                     /locus_tag="ACHUZQ_001653"
     CDS             26515..>26891
                     /locus_tag="ACHUZQ_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1025374.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKES"
BASE COUNT         6677 a         6748 c         6718 g         6748 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggcttttttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct ggggccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc acgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttatctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcaaagac cttcttccct gacttctcta
     1561 aagtgggcac cctggttgta tttgttgcct tcattttgag ttatatgggc gtagaagcat
     1621 ccgcaaccca cgtcaatgaa atgagcaacc cagggcgcga ctatccgttg gctatgttac
     1681 tgctgatggt agcggcaatc tgcttaagct ctgttggtgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcacagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggct attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 actctaccga tatgtatgtt gaattactgg tcgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgctcgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcatatat ggatatctgc
     2641 tcccgaaacg agaaattagc gattagaaga ccagcgatac tggttgcact ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtg
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaaccccac cagccgggcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tgcaacgtct cggtataaac
     2941 acggtctttt tccaggttaa gccggacggt accgccctgt ggccatcgaa aattttgcca
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattt
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc agaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga gtcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgc aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaaag tatcgcacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac tcccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtcct
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 aatctcgcac gttgcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta
     4321 tggagatgta ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc
     4381 cactcattcg tgctaaagcc gcggaaattg ccgttgcgca tgctcattat ctgagtatcg
     4441 agttttatcg aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag
     4501 ttgagcggca gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg
     4561 ttgacgatgt cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca
     4621 taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc
     4681 cggtaatctt ctcttcggat tattccgccg cagaaaaact tcaggtctat catttctcga
     4741 ttaacagtat tgatatcgcg atggaagtga tgacccgcgc gtttaccttt agtgacagta
     4801 gtgcctcaaa ggaagatgaa aattatcgta tcttctcgtt actggaaaac gccgaagaag
     4861 aaaaagaacg gcaaatagcc tcaatacttt catgggaaat agatattatc tataaaatcc
     4921 tgctggattc tgatttaggc agtagtttgc cattaagcca ggctgatttt ggcctgtggt
     4981 ttaaccataa aggtcgacat tattttagtg gcattgccga agtaggccat atctcccgtc
     5041 tcattcagga tttcgacggt attttcaatc aaaccatgcg taacaccagg aatttgaata
     5101 acagaagtct gcgggtgaaa tttttattac agataagaaa taccgtatcg cagattatta
     5161 ccttgctgcg tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactaa
     5221 cgaaattact taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca
     5281 accggaccgg tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca
     5341 acgatacgtg gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt
     5401 atgacaacgt ccacagtagt gattatgttt ttcgctacgg gggcgatgaa tttatcattg
     5461 ttttgactga agcttctgaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg
     5521 tggagaaaac caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg
     5581 gtgccgccat gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag
     5641 ctctgtatat cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt
     5701 agatgcgcca ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg
     5761 gcattttttt ccccgccctt gaacaaaata tgatgggcgc ggtgttaatt aacgaaaatg
     5821 atgaagtgat gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag
     5881 tcattggcaa taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat
     5941 acattcgtca taaccgtgaa ggtggtaaag cgcgcgtcga ggggatgagt cgggagctgc
     6001 agctggagaa aaaagacggc agtaaaatct ggacccgttt tgcgctatcg aaagtgagcg
     6061 ccgaggggaa agtttattac ctggcgctgg tacgggatgc cagcgtagaa atggcgcaaa
     6121 aagaacagac ccgacaattg attattgccg ttgaccatct cgaccgaccg gtgattgtcc
     6181 tcgatccgga acgccatatt gtgcagtgca atcgcgcatt taccgaaatg tttggttact
     6241 gcattagcga agccagcggt atgcagcccg atacactcct gaacattcct gaattccctg
     6301 ccgataaccg cattcgttta caacagttgc tatggaaaac cgcccgcgat caggacgaat
     6361 ttctgctgtt gacacgcacc ggtgaaaaaa tctggattaa agcctctatc agcccggttt
     6421 atgacgtgct cgcgcatctg cagaacctgg taatgacctt ctcggatatc accgaagaac
     6481 ggcagatccg ccagcttgaa ggcaatattc tcgccgccat gtgcagcagc ccgccatttc
     6541 atgaaatggg ggaaatcatt tgtcgtaaca tcgaatctgt tctcaacgaa tcgcatgttt
     6601 cgctgttcgc actgcgcaac gggatgccga tacactgggc gtcatcttcg cacggtgcag
     6661 aagttcaaaa tgcgcaaagc tggtcagcga ccattcgtca gcgtgatggt gcgcctgcgg
     6721 ggatcctgca aattaaaacc tcgtcaggag cagaaaccag cgcctttatc gaacgcgtgg
     6781 cagatatcag ccagcatatg gccgcgctgg cgctggaaca ggaaaaaagc cgtcagcata
     6841 ttgaacaact catccaattt gatccgatga ccggtctgcc aaatcgcaat aacctgcaca
     6901 attacctcga tgaccttgtc gacaaagccg tctctccggt ggtgtatctc atcggtgttg
     6961 accatattca ggatgtgatt gatagccttg gctatgcgtg ggccgatcag gcattgctgg
     7021 aagtggtcaa tcgctttcgt gaaaaactca aaccggatca gtatctctgt cgtatcgaag
     7081 gtacgcagtt tgtcctcgtg agcctggaaa acgacgtcag taacattacc caaatcgccg
     7141 atgagctacg gaatgtggtc agcaagccga taatgattga cgataaacca ttcccgctaa
     7201 ccttgagtat tggcatcagc tacgacgtgg gtaaaaaccg cgattacctg ctctccactg
     7261 ctcacaatgc aatggattat attcgcaaga atggcggtaa cggctggcag ttcttcagcc
     7321 cggcgatgaa cgaaatggta aaagagcgtt tggttttagg cgcagcgctg aaagaagcga
     7381 ttagcaataa ccaactgaaa ctggtttacc agccgcaaat cttcgcagaa acgggtgaac
     7441 tgtacggcat cgaagccctt gctcgctggc acgatcccca gcatggtcat gtaccccctt
     7501 cacggtttat tcctctcgca gaagagattg gtgaaatcga aaatattggg cgctgggtca
     7561 tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca gaatattcat atcccggcgt
     7621 tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca gctgcctaat caggtgtctg
     7681 atgcaatgca agcctggggt attgacggcc accagctgac ggtagaaatc acggaaagca
     7741 tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgc gatatgggcg
     7801 tgggcttatc ggtagatgat tttggcacgg gcttttccgg attatcccgc ttagtcagtc
     7861 ttccggtaac ggaaatcaaa attgacaaaa gttttgtcga tcgttgtctg acggaaaaac
     7921 gcatccttgc cttacttgaa gccattacca gcattgggca aagcctcaat ttaaccgtcg
     7981 tggcggaagg cgtcgaaacc aaagagcaat ttgagatgct acgcaagatc cactgtcgcg
     8041 ttattcaggg atatttcttt tcccgccccc tacccgccga ggaaattcca ggctggatga
     8101 gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc gggttttcat
     8161 ttaacgtgac actgtcactt aaatactgtg atttcagcta cgattccggg agattccttc
     8221 cttaacttcc cctcttgcac caaaactgca ctacgctgca caaatgttga tcaaaagtta
     8281 aaaaatataa ctttaagcca gcattctggc gcttatcccg gcatggcatg agatctgcat
     8341 aagcggaaag cgcagcaatt tttgtcttaa tacaggaacc gtttatgtcg gataccaccg
     8401 aactggttga tttagccgtg atcttccctg atctggagat cgaattgaaa tacgcctgcg
     8461 ctgataacat cacaggtaaa gctatttacc agcaagcgcg ttgtctgtta cacaaggatg
     8521 cgattaccgc gctggcgaaa agtatcagta tcgcccagct gtcaggctta caactggtga
     8581 tttacgatgc gtatcgccca caacaagcac aggcgatgtt gtggcaagcc tgcccagacc
     8641 cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc
     8701 tgacgcttcg tgatgagcac gggaacatcc tggatatggg cgcagggttc gatgaaatgc
     8761 acgagcgctc ccatgcctat cacccttccg tcccgcccgc cgctcagcgc aatcgcctgt
     8821 tgctgaatgc gataatgact ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact
     8881 tcgaattacc ccaggcagcg agttaccctc tgctcgctga tcaattctct tgttttatat
     8941 cccctggcac acagcacgtc agttaatcag gaaactgtca tgaagagatc gatatcgttt
     9001 cgtcccacat tgctcgcgct cgtccttgcc acaactttcc cggttgcgca cgccgccgta
     9061 ccgaaagata tgctggtgat tggtaaggcc gccgatccac aaaccctcga cccggcggta
     9121 acaatagata ataacgactg gacagtgacc tacccgtctt atcagcggct ggttcagtac
     9181 aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg atctggcaag tagctggaaa
     9241 gcgtctgacg atcaaaaaga gtggacgttc accctgaagg acaatgctaa atttgccgat
     9301 ggcacacctg tcactgccga agcagtaaaa ctctcttttg agcggttact aaaaatcggc
     9361 caggggccag cagaagcatt tcccaaagat ttaaagattg atactcccga cgaacatacg
     9421 gtgaagttta cccttagcca accattcgca ccgttcctct acacgctagc gaatgacggt
     9481 gcatccatta tcaatccggc ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc
     9541 ctcgcgcaaa ataccgccgg ttccggacca tttatgctga aaagctggca aaaaggtcag
     9601 caattagttc tggtgccaaa tccgcattac cccggcaata aaccgaactt taagcgagta
     9661 tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt
     9721 gacattgccg atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc
     9781 aatgtggcag agtatccgtc actgcgcgtt acctatctgt atctgaataa cagcaaagcg
     9841 cctcttaatc aggcggatct gcgtcgggcc atttcctggt ctaccgatta tcagggcatg
     9901 gttaacggca ttctgagtgg taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg
     9961 tggggctacg atgcgacggc aatgcaatac aaccatgacg aaacgaaagc caaagccgaa
    10021 tgggataaag tgacgagcaa acccaccagc ctgacgtttc tctactccga taacgatccg
    10081 aactgggagc ctattgctct ggcgacacaa tccagtctca acaagctggg catcaatgtg
    10141 aagctggaaa agctggcgaa cgccaccatg cgcgacagag tgggtaaagg tgattacgac
    10201 attgcgattg gcaactggag tccggatttt gccgacccgt atatgtttat gaattactgg
    10261 tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc
    10321 gataagttac tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac ccgggactac
    10381 cagcaggcac agaaaatcgt cattgatgac gctgcttatg tgtacctgtt ccagaaaaac
    10441 taccaactgg cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat gctggaacag
    10501 gtcttcaata tcaataccat gagtaaataa cgtgaccttc tggagtattt tacgccaacg
    10561 ctgctggggg ctggtgctcg tggtggcggg cgtctgcgtg attacgttta ttatctcgca
    10621 tctgatccct ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg atgctatcgt
    10681 ggaaaatatt cgccagcaac tgggactgga ccagccactg tatgtacagt tttaccgcta
    10741 cttcagcgac ctgtttcatg gtgacctggg aacatccatt cgtaccgggc gtccggtgct
    10801 ggaagagttg cgtatatttt tcccggcgac gctggaactg gctttttgtg ccctgctgct
    10861 ggcactcctg attggcatcc cgctgggcat actctctgca gtctggcgaa atcgctggct
    10921 ggatcatctg gtgcgaataa tggccattac cggaatctcc acacctgcgt tctggcttgg
    10981 actgggcgtc attgtgctgt tttatggtca tctgcaaatt cttcccggcg gcggaaggct
    11041 tgatgactgg ctggatccac caacgcacgt taccggcttt tatctgctcg atgcgctgct
    11101 tgaaggcaac ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac cggcattaac
    11161 gctggcgttc gttcacctgg gaattgtcgc tcgccagatc cgctcagcga tgctggaaca
    11221 attgagtgaa gactacattc gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt
    11281 tttatgttat gcgctaccca atgcgttgat cccatcgatt accgtattgg gtctggcgct
    11341 gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg
    11401 tgcatgggta gtaacatcaa tacaggcgct cgacttcccg gcagtgatgg gctttgccgt
    11461 cgtggtttca tttgcttatg tgctggtcaa cctggtggtg gatttgctct atttgtggat
    11521 tgatccgcgt atcggacgtg gaggtggtga atgatgctaa gcgaggaaac gtccgccgta
    11581 cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg tatggatgct gaaaggcagt
    11641 ccgctcaccg tgaccggcgc agtcatcatt gtattaatgc tattgatgat gattttttca
    11701 ccgtggcttg cgacgcatga tcccaacgcc attgatttaa ccgcccgcct tttgccgcct
    11761 tctgcggcgc actggtttgg caccgatgaa gtgggacgcg atctgttcag ccgcgtactg
    11821 gtcggcagtc agcaatcaat tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt
    11881 ggttcgctac tcggatgtct atccggtgtg cttggcggac gcgcagacgc catcatcatg
    11941 cgcatcatgg acattatgct gtcgattcct tcgttggtac tgacaatggc actggctgcc
    12001 gctctcgggc cgagtttatt taacgccatg ctggcgattg ctattgtgcg aattcccttt
    12061 tatgtgcgcc tggcgcgggg gcaagcatta gttgtacgcc agtataccta tgttcaggcg
    12121 gcgaaaacct ttggtgcgtc gcgttggcat ctgatcagct ggcatatttt acgtaactcc
    12181 ctgccgccgc tgatcgtgca ggcatcgctg gatatcggta gcgcgatttt aatggccgcc
    12241 acgttgggat ttattggcct gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta
    12301 gctaatggtc gcaactatgt actcgatcag tggtggtatt gcgcatttcc gggggcagcg
    12361 attttgctta ccgccgtcgg gtttaatctc tttggtgatg gtattcgcga tctgcttgac
    12421 ccgaaagcag gaggaaagca gtcatgaccc aacccgttct ggacattcaa cagctgcatt
    12481 tgagtttccc cggttttaac ggttatgttc acgtgctcaa caatgtgtcc ttgcagatta
    12541 accgcggtga aattgtcggt ctggtgggag aatccggctc aggtaaatca gtcaccgcaa
    12601 tgctgattat gcgtctgcta ccgacgggca gttattgcgt acatcggggg cagatttcac
    12661 tgctgggaga cgatgttctt aacgctcggg agaaacagct tcgtcagtgg cgcggcgcac
    12721 gagtggcgat gatctttcag gaaccgatga ccgccctcaa tccgacacgt cgaataggtc
    12781 ttcagatgat ggacgtgatc cgccatcatc aaccaataag tcgtcgggaa gccagagcta
    12841 aagcgattac cctgctggaa gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct
    12901 atccgtttga gctttcaggt ggtatgcgcc agcgggtaat gattgcgctg gcattctcct
    12961 gcgagccgca attgattatt gccgacgaac cgactacggc gctggacgtc acggtacagt
    13021 tgcaggtact gcgtttgctt aaacataaag ctcgcgccag tggaactgcg gtactgttca
    13081 tcagccatga tatggccgtg gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg
    13141 gaagcgtaat tgaaagcggc gtgacggcag acgttatcca tcatccccgg catccgtata
    13201 ccattggttt gctgcaatgc gcaccagaac atggagtacc acgccagcca ttacccgcca
    13261 ttccagggac ggtaccaaac ctcacccatt tgcctgacgg ctgcgctttt cgcgatcgtt
    13321 gctatgcggc aggtgcacag tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca
    13381 accagcgctg cgcctgttgg tatccgcagc aggaggtcat aagtatctga cacgttatta
    13441 acgttacgcg acgtccatat caatttcccg gcccgtaaaa gctggcttgg taaaactacg
    13501 gaacatgttc atgcgattaa tggtattgat ttacagatcc gccgtggtga aaccttaggg
    13561 atcgtcggtg agtcaggctg cggcaaaagc accctcgcac agcttttaat gggtatgctg
    13621 caaccgagcc acgggcaata catccgttca ggatcacaac gcattatgca gatggtgttt
    13681 caggacccgc tctcttcgct taatccgcgc ttatcggtgt ggcgcatcat cacagaaccg
    13741 ctctggatag ctaagcgtag tagtgaacaa cagcggcgag cgttggcaaa ggagttggct
    13801 gtgcaggtgg gtattcgtcc ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg
    13861 cagcggcaac gcatcgccat tgccattgcc agagcactct cttcgcagcc tgacgtgatt
    13921 gtgcttgatg agccaacctc ggcgctggat atctccgtgc aggcgcagat cctcaattta
    13981 ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg
    14041 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    14101 ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat
    14161 tccctccccg ccattgataa accgctggag gaagaatggg cattatgtaa aacggatctg
    14221 ccgggaaacc gcacgttgcc acaaggctgt tttttctacg aacgttgccc tctggcaacc
    14281 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc
    14341 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    14401 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    14461 aaaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    14521 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaatcg gcatccactt
    14581 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    14641 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    14701 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    14761 cgccactctg ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    14821 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    14881 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatacgg cgaaaatatt
    14941 acttctgcac ttaaattaaa ttcccacaat agataaccgg gaatatatat ctcaagagat
    15001 attgcatcaa tatcaatcaa ctcaccacaa taataataca tatcaatatc atggagttaa
    15061 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    15121 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    15181 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    15241 gccattgagc aaggccttcg ggcagagcaa ggtgttgtca ctgaagatga cattctcatg
    15301 gagctgacca aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    15361 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    15421 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    15481 agcaacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    15541 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg
    15601 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    15661 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    15721 tggggccgac gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc
    15781 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    15841 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    15901 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    15961 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    16021 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
    16081 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    16141 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata
    16201 tggacgatat tctgcaaaac gtaccgaacc ataatatcaa agtgattgtg gtgactgacg
    16261 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    16321 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    16381 tggatgtcgg aacgaacaac caacaactgc ttaacgatcc gctgtatatg ggctggcgta
    16441 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    16501 aacaacgctg gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    16561 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
    16621 cggcggtaac agtcggcaca ctgatcgcag caagccgagc ggcaggtggt cagttaagcg
    16681 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    16741 tcgcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    16801 tcgatcgctt tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac
    16861 tggtgcagaa acgcgaaaac ctcagtgact gggataccga cagcgatgtg ctgtcactgc
    16921 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    16981 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    17041 cgctgtctaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggaccg
    17101 aaggtaacgc gctggtcgcc acgggcagcc cgtttaatcc agtggtatgg aaagataaaa
    17161 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggggtta
    17221 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
    17281 cacagtattc gccgctggtg ctgaacggcg aaggtctggt actaccggaa ctaaaagata
    17341 ttcagaaagt ctcccgtgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    17401 tggcggtgaa aacctctgcc gaagctttgc aacaagccat tgacgataat ttctggcaag
    17461 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    17521 cgggcaattt tcctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    17581 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    17641 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
    17701 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    17761 aatggcgatt ttggtgacaa aaccgacgta attctgggcc atgaaggcat cggtgtggtg
    17821 gcagaagtgg gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    17881 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    17941 tcagttaaaa atgccggata cagcgttgat ggcggaagag tgcatcgtgg tcgccgatta
    18001 cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg
    18061 ggtcaccacc tacaaagccg ttaagctgtc aaaaattcgt cccgggcagt ggattgctat
    18121 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg tctttaacgc
    18181 caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggtgc
    18241 agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg
    18301 tggcgctcac tctgcggtgg taacagcagt agctaaagct gcgtttaact cggcagttga
    18361 tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct
    18421 ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg
    18481 ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
    18541 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
    18601 tggccgtatg gtgattgatt tccgtcacta agaggtcttt gctgcgactc ccatgttcgg
    18661 gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt tgcgtaggaa
    18721 ggaacaataa tgatagtgtt cccagagaag gaacgctact gagagtaaag acactaatga
    18781 aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac gaacttaatg
    18841 tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca agccgattgc
    18901 tgaccggaaa agcagctttg acgccagaaa tggcaattaa actttccgtg gtgatcggca
    18961 gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag gcagaaaaaa
    19021 cggtggatgt gtcgcgatta cgccgtctgg ttacgcaata aaaaatggca ccttataaag
    19081 cgccatttga aactgatgac aaacgcaaca ttgcctgatg cgctacgctt atcaggccta
    19141 cacgttcact tcaatctatt gtatttgaag ggttttcatg ccggataagg cattcacgac
    19201 gcatccggga tgaacaaagc ccacgttgtc tacaatatga aaatggcgcc ttttacagcg
    19261 ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt
    19321 ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca
    19381 ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga
    19441 tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa
    19501 caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca
    19561 gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact
    19621 taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc
    19681 acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca
    19741 gtgcgacgaa aatcgcaatg ccgaagaacg ggtgcaaaat gcgtcccatc tgcggcgtac
    19801 cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca
    19861 ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca
    19921 caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg
    19981 ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc
    20041 agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc
    20101 agaccgtaat acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca
    20161 ccgacccctt tcgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc
    20221 acgcgctctt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc
    20281 ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg
    20341 catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca
    20401 atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac
    20461 tgaatgattg caccagcaga cgggcacgcc ttcaggcagc cgggatcttc acagtgcata
    20521 cagccgtctt tacggatcag ccactccagc ttgccgttct gttcggtttc gctaaagcgc
    20581 atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc
    20641 cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg
    20701 atacaggtgg aaacgtcgat aagttttgcg acttctgctt tgtaatcacg cacctgagaa
    20761 ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc catagccatc
    20821 tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc
    20881 gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca
    20941 ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc
    21001 agcggtttca gacggcgcgt taccaccgcc accgcgcgga taaagccacg cttgcttgag
    21061 acagtgacac gatcgccatt attaatgcct ttcgccgccg ccagcgtttc gctgatttcc
    21121 acaaactgtt ccggctgagc aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag
    21181 tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc
    21241 agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg
    21301 ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt
    21361 ttgttgatgg caaacaggcg tcccatccct tccggctgca tgataaacgg cccggttggc
    21421 gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt gctgccgttc
    21481 cactggatca gcatccgttt cggatcccac ggtttaccat tgatatccgc cgaagcacgg
    21541 ttgtagagca cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga
    21601 ccggatgggt cggagttatc gcggttagcc atctggttgc cctgctctgt ccagctaccg
    21661 gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc
    21721 agcaactgac ctttcttcgc aatcagcacg ccattagcgt catagagatc ttccagcgca
    21781 tagccgttat tctctttcgc cacttcgtca gattgcggtt cgtgcggctg cttgtagttc
    21841 cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta cagctcgcgc
    21901 agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc
    21961 tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc atcttcttcg
    22021 gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg
    22081 ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc
    22141 accatgtact tcagcttgct caggcagctc accactttgt ttttgtccgg gaaggacgca
    22201 accgggttaa agccctggca gaagtaaccg gtgactttgc cttcgtccat catgttgaaa
    22261 tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc
    22321 tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag
    22381 ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac
    22441 gactgcaaat caacctgttt ttctgacgga agcgtcagat aacctggcag gctggtagag
    22501 agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc gttcacgccg
    22561 ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc
    22621 tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc
    22681 ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt tggcgtaccg
    22741 cagatgtttt caacgacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac
    22801 acacagcgcg gatgagtcag tgtttcatcg cgttttgcat agccgttttc atcgaactga
    22861 tagttccagg acgacttatc atactggcgt ttttcagcgt cgtagccgct gaacaggccg
    22921 tcttcgaaag caaaatcatc acgcaccagc aggctggcgt tggtgtaatg tttaacgtat
    22981 tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg
    23041 tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacga
    23101 ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa
    23161 ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg
    23221 atatccaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc
    23281 gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca
    23341 aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta
    23401 gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg
    23461 tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat
    23521 ttgtcagaac ctggcgcacg atattccggg tagcgcagac ggttttcgct gttgacgtaa
    23581 tccagcaaac cggccccttt tgggcacagc gcaccacggc ttaccggatg atccgggtca
    23641 ccttcaatgt gataaatcgc ttctctggcg tttttcgcgc catcacccag gctatacatc
    23701 aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat
    23761 aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc
    23821 gttgttccag ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt
    23881 gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc
    23941 tttttgcgcg aaatcaaaaa gaacacgtgt atgccacaaa ataatccgcc tgcgcaaatt
    24001 aacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg
    24061 ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac
    24121 aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga
    24181 ttcgcggtgt cagcgagggg ctcggtccgg tcggcggcgc agctgctatc tattcattaa
    24241 gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct
    24301 atttactcgc cgggagtctg ttattcgtca gctatgaaat ctgtctggcg ctttccttag
    24361 ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca
    24421 gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac
    24481 caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac
    24541 attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta
    24601 ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta
    24661 acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac tattttctca
    24721 cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat
    24781 ttaccttagg atttgcttat gctgcatgga atgtcggtat attgcatggc aatgtcacca
    24841 taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca gccgtgctgc
    24901 tcagcgcccc gctgtcgttc tcgttctggc aaggcgcgct aatggtctgc ggcggttccc
    24961 tgctctgctg gctggcgaca cgtcgtggtt aaaataattg ccgggtctac ggcccggcag
    25021 ttaatcagat aaattccgac aatattaaaa aataaatttc aaataccagt aattcacctt
    25081 atatctcatt tcgacttcga tcacatatcg ataaataata ttatttcatc tatttttctc
    25141 attgttttta atcaccattc tattttaaaa acagtatgtc atttataaat gacgccgttt
    25201 aaaattcgtt tagaaaatac gtttaacgat aattaaaagg attctgttaa atgaaattaa
    25261 aaatagttgc ggtggttgta actggtttgt tagctgcgaa cgtagcacac gctgccgaag
    25321 tctataacaa ggatggtaat aaactcgacc tttatggcaa ggttaccgct ctacgttatt
    25381 ttactgatga taagcgtgac gatggtgata aaacttatgc ccgtctcggc tttaaaggag
    25441 aaacgcaaat caatgatcaa atgattggtt ttggtcactg ggaatatgat tttaaaggct
    25501 ataacgatga agccaacggc tcgcgcggca acaagacccg tctggcctat gctggtttaa
    25561 aaattagtga atttggctct ctggactatg gtcgtaacta cggtgtcggc tacgacattg
    25621 gttcatggac cgatatgttg ccagaatttg gtggcgatac ctggagtcag aaagatgtct
    25681 tcatgacata ccgtaccacc ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa
    25741 ttgaagggct gaactttgcc gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc
    25801 atctttatgg tgctgactac acgcgtgcca acggtgacgg tttcggtatc tcctcaactt
    25861 atgtttatga tggctttggt atcggtgcgg tatataccaa atccgatcgg acaaatgcgc
    25921 aggaaagagc cgctgctaat cctctcaatg cctccggtaa gaatgcagaa ctatgggcca
    25981 caggtataaa gtatgatgcc aacaacatct actttgcagc taattacgct gaaacattaa
    26041 acatgaccac ctatggggat ggttatatct ctaacaaagc acaaagtttt gaagtggtgg
    26101 cgcaatatca attcgacttc ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca
    26161 gagatctggg ccgctacggc gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt
    26221 atttcttcaa caaaaatatg tcgacctatg ttgattataa aatcaacctg attgatgaaa
    26281 gcgactttac ccgtgccgta gatattcgga ccgataacat cgtcgctacg ggcattacct
    26341 atcagttcta acgtcctctc ctgaccagcc cttctctggg ctggtttttg atttaagtgc
    26401 tgctgtatga ctccttaagt aaaaatgagc aatttttgat atttctggtc attcacctca
    26461 attaatatta ttccatatcc tccggggaca tcttactata atatttattg atgagtgatt
    26521 atgaccaata ttaatacagc ttgtgtaaaa aataatgcca gttatcaact taataacgca
    26581 ttacctaaca aagaaaccat ctccagcaat ttttgtgaac gactagaaca atggggtaat
    26641 aaatcggtta ataatggtga agaaagagca attgccgtgg agcgaattaa agaagcttac
    26701 aattcgaaca tggcatctct tgatttgtca tatcttgatt tgagtgaatt accgcctatc
    26761 ccctcgacag ttaatacgtt aaatctggaa aacaattgtc taacttgtct tgacttagct
    26821 gacaatgcca gtctcgttaa tatcaacctc agcgttaata aaattaacac gataaccttt
    26881 ccaaaagaat c
//
LOCUS       DAZPBM010000043        25637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPBM010000043 DAZPBM010000000
VERSION     DAZPBM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.043"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHUZQ_001654"
     CDS             complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHUZQ_001654"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1025375.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHUZQ_001655"
     CDS             complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHUZQ_001655"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1025376.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHUZQ_001656"
     CDS             complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHUZQ_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1025377.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHUZQ_001657"
     CDS             complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHUZQ_001657"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1025378.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5488..5685)
                     /locus_tag="ACHUZQ_001658"
     CDS             complement(5488..5685)
                     /locus_tag="ACHUZQ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1025379.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHUZQ_001659"
     CDS             complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHUZQ_001659"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1025380.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHUZQ_001660"
     CDS             complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHUZQ_001660"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1025381.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHUZQ_001661"
     CDS             complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHUZQ_001661"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1025382.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHUZQ_001662"
     CDS             complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHUZQ_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1025383.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHUZQ_001663"
     CDS             complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHUZQ_001663"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1025384.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHUZQ_001664"
     CDS             complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHUZQ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1025385.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHUZQ_001665"
     CDS             complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHUZQ_001665"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1025386.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHUZQ_001666"
     CDS             complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHUZQ_001666"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1025387.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHUZQ_001667"
     CDS             complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHUZQ_001667"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1025388.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16005..17144
                     /gene="queG"
                     /locus_tag="ACHUZQ_001668"
     CDS             16005..17144
                     /gene="queG"
                     /locus_tag="ACHUZQ_001668"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1025389.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17127..17180)
                     /locus_tag="ACHUZQ_001669"
     CDS             complement(17127..17180)
                     /locus_tag="ACHUZQ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025390.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17414..17489)
                     /locus_tag="ACHUZQ_001670"
     tRNA            complement(17414..17489)
                     /locus_tag="ACHUZQ_001670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17454..17456),aa:Gly,seq:gcc)
     gene            complement(17525..17600)
                     /locus_tag="ACHUZQ_001671"
     tRNA            complement(17525..17600)
                     /locus_tag="ACHUZQ_001671"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17565..17567),aa:Gly,seq:gcc)
     gene            complement(17758..17833)
                     /locus_tag="ACHUZQ_001672"
     tRNA            complement(17758..17833)
                     /locus_tag="ACHUZQ_001672"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17798..17800),aa:Gly,seq:gcc)
     gene            complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHUZQ_001673"
     CDS             complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHUZQ_001673"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1025391.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHUZQ_001674"
     CDS             18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHUZQ_001674"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1025392.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19833..20801
                     /gene="asd"
                     /locus_tag="ACHUZQ_001675"
     CDS             19833..20801
                     /gene="asd"
                     /locus_tag="ACHUZQ_001675"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1025393.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20823..24146
                     /gene="mscM"
                     /locus_tag="ACHUZQ_001676"
     CDS             20823..24146
                     /gene="mscM"
                     /locus_tag="ACHUZQ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1025394.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHUZQ_001677"
     CDS             complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHUZQ_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1025395.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5928 a         7193 c         6501 g         6015 t
ORIGIN      
        1 tatcttacca gctatagaca aaaaaaccat ccaaatctgg atggcttttc ataattcaga
       61 cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt
      121 tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg
      181 tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaagcgt ccggtcattt
      241 tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt
      301 cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg
      361 ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga tctttcggca
      421 caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg
      481 gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
      541 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
      601 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
      661 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
      721 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
      781 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
      841 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
      901 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
      961 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
     1021 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
     1081 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
     1141 tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
     1201 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
     1261 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata
     1321 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
     1381 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
     1441 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1501 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
     1561 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
     1621 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatact taatggcgcg
     1681 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1741 tgccaggcca aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag
     1801 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
     1861 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
     1921 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1981 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     2041 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
     2101 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
     2161 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     2221 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     2281 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
     2341 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg aacacaggcc
     2401 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc
     2461 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
     2521 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2581 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
     2641 gcccgctttt gcttcttccg gcacttcttc tttcagcccc gcaacctgtt gctcaacagc
     2701 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2761 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc ggcgagtcgg
     2821 acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2881 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2941 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     3001 cgcttcacga cgacctttac ggtcagcacc cagcggctgc gccagcacct gatcgccatg
     3061 aatacaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     3121 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
     3181 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     3241 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     3301 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     3361 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3421 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3481 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3541 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3601 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3661 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3721 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3781 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3841 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3901 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3961 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     4021 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     4081 cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc cggaccggta
     4141 gagatgatat cgatcggcac accggtcagc tcttcaatac gcttgatgta gttcagcgcc
     4201 gcctgcggca ggccgctacg atctttcacg ccgaaagtgg attcagacca gcccggcatg
     4261 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagccact
     4321 tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag gccatccaga
     4381 acgtccagct tggtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4441 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcgcc gaattcgtta
     4501 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa cggacctgca
     4561 cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata acgcgggccc
     4621 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4681 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
     4741 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4801 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4861 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4921 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc ataagcaggc
     4981 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     5041 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     5101 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     5161 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     5221 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     5281 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     5341 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     5401 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5461 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5521 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5581 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5641 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5701 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5761 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5821 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5881 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5941 ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca gcgttctggt
     6001 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     6061 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     6121 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     6181 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     6241 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
     6301 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatgtc
     6361 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6421 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6481 gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg tcacaaagcg
     6541 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6601 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
     6661 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6721 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6781 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6841 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6901 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc gcctttcagc
     6961 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
     7021 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     7081 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
     7141 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     7201 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     7261 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agctgcctgg
     7321 aagttgacgt ccagcagagt gatacccatg tcatacggac ggatagtctc ttccagttcg
     7381 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7441 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7501 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7561 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7621 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7681 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7741 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7801 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7861 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7921 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7981 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     8041 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     8101 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
     8161 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
     8221 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgtc cttcctgcgg
     8281 cggcaaacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     8341 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     8401 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8461 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8521 ttcttgtaca cgtacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8581 ctcttgtaac gtggctttaa atgctgccac cagatcgtgc ggcaggtggc gaataaaccc
     8641 tacggtatct gcaagtacgg tttcaccgac atccgcgacg tcaatacgcc gcaacgtcgg
     8701 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8761 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8821 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttctaggc gcgactgtat
     8881 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8941 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     9001 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     9061 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     9121 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     9181 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     9241 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     9301 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     9361 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9421 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
     9481 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
     9541 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
     9601 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac aggatcacga
     9661 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
     9721 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
     9781 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
     9841 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
     9901 acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc ttcccaacca
     9961 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    10021 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    10081 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    10141 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    10201 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    10261 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    10321 ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc ctgaagttga
    10381 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    10441 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    10501 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc cgccagcgca
    10561 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    10621 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    10681 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    10741 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    10801 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    10861 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    10921 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    10981 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    11041 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    11101 atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact gcgctttttc
    11161 taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaataagca gcggctgagc
    11221 gcaaactggc gcttcacccg gcgtcagttg tacctgacgc agccaacgtt ctgccactgg
    11281 caaggctaaa agtgaaatgt tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat
    11341 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    11401 cgcttttaat ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    11461 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgatgc
    11521 tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    11581 gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg gttcatcgtc
    11641 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    11701 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gcacgttgac
    11761 gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct gatcggcccc
    11821 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    11881 accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc
    11941 cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcaag
    12001 aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    12061 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    12121 cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct cggtgcgcag
    12181 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgtccccac
    12241 aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca
    12301 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    12361 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    12421 gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt tgataccgca
    12481 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    12541 cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    12601 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    12661 caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc
    12721 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    12781 gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    12841 gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg aacgcagaac
    12901 gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact
    12961 gatcatactg gtcgctacat cataccctac ccgctgggaa tgaccgaact gtaaatccag
    13021 caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcgc ccgccccacc
    13081 cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc tgttggcgcg
    13141 acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg cggcatcagc
    13201 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcgctgc gccccatcac
    13261 cgagataaag taatccccgt cacgggttaa aacgccttta aacatcggat cgtcattgag
    13321 caaagtgcgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    13381 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    13441 gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc tgataacacc
    13501 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    13561 gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggtggaggag gcggtggcgg
    13621 cacatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    13681 ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    13741 cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg gcagtccctg
    13801 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    13861 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    13921 ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca gcgtcgctac
    13981 caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac cggctaaacg
    14041 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    14101 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac cttgttgtgg
    14161 ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca taaactccag
    14221 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat tgtcgagcgt
    14281 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac ccagagcttg
    14341 taaaaaaccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    14401 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    14461 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    14521 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    14581 gtacgtcagc tgccgcaccg tgcgcgacac agcccgcaca ggcggcatca tacggcgaca
    14641 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    14701 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    14761 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    14821 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    14881 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    14941 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt gcttttttcc
    15001 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    15061 cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca gtcagcagcg
    15121 gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca cgcagcgccg
    15181 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata atcaccagcc
    15241 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt cagccagtga gaaagttgtt
    15301 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccagctatcc agccccagtg
    15361 agtcaaaatg cagttgtcct gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    15421 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    15481 cagccagcag gccggaaggg atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt
    15541 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcagcg
    15601 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgccac
    15661 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    15721 cctgggccaa caacgtgacc tcaatgccga ccgctttggc cagtcgcgcg accacgtagc
    15781 catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg
    15841 ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag catcagctca tagagtgtga
    15901 gccccagcac atctgccgcc tcgcgttctc cgcggcggat atcgtcggcg taccagacgg
    15961 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    16021 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    16081 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    16141 aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc gctcgccccc
    16201 atgagttatt accaggtacg ctgcgtgtaa tcagcgtgcg gatgaattac cttcctgcta
    16261 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    16321 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    16381 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    16441 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    16501 gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg ctgcccgtgg
    16561 atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt tgcccgaccg
    16621 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    16681 aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    16741 gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact acagaagacg
    16801 atttcagccc gcgtaagccg ctacacgcac aggaactcat tgagttattt gcctggagcg
    16861 aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt cacctgcgct
    16921 ggctgcgtaa tatcgccgtg gcattaggca atgcgccctg ggatgaaacg attctggcgg
    16981 cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg tgggcgattg
    17041 cgcagcaaat cgagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    17101 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    17161 gaataaaaat aaaaacgcat tgcaattcaa gacgcataaa atcgtcaagt gatcgactcg
    17221 acaaaacaga atcttgattt ttaacttatt tatcaatagg ttaaaaaaat accattcacc
    17281 gtgcgaagtt tctttgaaat taagattaca gcctgaggat gtggataact ctgtttacaa
    17341 gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg gatattttat
    17401 tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    17461 tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca gcactttcaa
    17521 attttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct
    17581 accaactgag ctattcccgc ttaatcttcg tctttcgaac cgctactgcc ttggctgcct
    17641 tcactcacct tagtcacata cttttgtaag ttcccaagga ttcgttcagt tgccgccttg
    17701 tcgcaattcg aaatactgcg attgggtggt ctgtgctttg cagcactttt caaattttgg
    17761 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
    17821 gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta
    17881 cgggaggcgc attatacgag aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg
    17941 cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt caaaatcacg
    18001 ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc ttgataaaat
    18061 gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatt gcctgatgcg
    18121 tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc agctctttca
    18181 gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc atgtatttaa
    18241 acaggaaacg acggtcctgt ccgatgctgt taccgcaaat cggcgatttt cccgcaggca
    18301 cccactgttt taagaattcg agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg
    18361 ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag tcatccatca
    18421 gcgccagctg ttcatcggac tgatgtactg caatggtcgg cccttctgcc agaatattca
    18481 gattggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg ggatccagac
    18541 cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg ctattttcca
    18601 cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt ttgcccatca
    18661 ggggcgacca ggagtcagta cgattgagta aaaataaact ctccaaaggc cagcagcgcc
    18721 gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc gactacgacg
    18781 acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt atgcacgctg
    18841 atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc atccgttcgc
    18901 tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg
    18961 tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt ccggatttct
    19021 acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc tccgccattt
    19081 tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa accttgcaga
    19141 ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc atggcgttcg
    19201 tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg gtttccagcc
    19261 atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc agcatttttg
    19321 ccgggcagtc tggcgtcggc aaatccagcc tgctgaacgc gttgctgggg ctgcaaaaag
    19381 agatcctgac caacgatgtc tctgacaact cggggcttgg tcagcacacc acgaccgccg
    19441 ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt gtgcgtgagt
    19501 tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact
    19561 atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc tgcgctatcc
    19621 gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat caccgtattc
    19681 tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat gactgacaat
    19741 taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt aggccgaatc
    19801 aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac
    19861 agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca agcaagcggg
    19921 caggatggtt gacaaaactg gttatcgatc tgttcgttaa atactacaag gtcgacatga
    19981 aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc tttgtccgtc
    20041 cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtactggtc atgcctgccg
    20101 atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc
    20161 acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac ctgttccgca
    20221 acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccaccgcgta cacatgccgt
    20281 gcaacggtat tctgcgtgag atgatctacg tgccgggcga tctcttctcc gttaaccatc
    20341 tcacggctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt tgccttttcg
    20401 ataccgaatt tggcccaatg gcgcagattc tggtcggagc gacgattgtt ggcagcattg
    20461 agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag cgctggacct
    20521 ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa atgggtcgct
    20581 ttaaactcgg ttccaccgtt atcaacctgt ttgcaccggg taaagtgaat ctggttgagc
    20641 aactggaaag cctgtctgtc acgaaaattt gccagccgct ggcagtatct accgaaacct
    20701 ttgttacgcc agacgctgaa cctgccccgc ttcctgctga agagatcgaa gcagaacacg
    20761 acgccagccc attggttgac gacaaaaaag accaggtcta atccatcaaa ggaaacgctg
    20821 acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg gcgtacgccg
    20881 cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa gcggcgaaac
    20941 ccgcacagcc ggaagtcgta gaggcgcttc agtctgcctt aaatgcgctt gaggaacgaa
    21001 aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat ccgaaactct
    21061 ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacaa gccgcgcagc gtgtcgccgg
    21121 gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag ttgctggata
    21181 aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat tcgctgaatc
    21241 aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg
    21301 gaacgcttac cggcaatact ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact
    21361 ctgcacgtct taaggcgctc gttgatgaac tggagctggc gcagctgtct gccaataacc
    21421 gccaagaatt agcgcgctta cgctcagagc ttgcggaaaa agagagccag caactggatg
    21481 cgtatttgca ggccttacgt aatcagttga atagccaacg tcagcttgag gcggagcggg
    21541 cgctggaaag taccgaatta ctggcagaaa acagtgccga tttgccgaaa gatatcgtcg
    21601 cgcaattcaa aattaaccgc gaactatcgg cagcgctgaa tcaacaggcg cagcggatgg
    21661 atctcgttgc ctcgcaacag cgtcaggctg ccagccagac gttacaggtc cggcaggcgt
    21721 tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caatctgctc ggtgaagcgt
    21781 tgcgagcgca ggtggcacgg ctaccagaaa tgccaaaacc acaacagctt gataccgaaa
    21841 tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa cagccgctgc
    21901 tacggcaaat ccatcaggcc gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg
    21961 aagcacaact gcgcactcag cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc
    22021 tactattgga actgaccaag ctgaaagtct ccaacgggca actggaggat gcgctgaaag
    22081 aggtgaacga agcaacgcac cgctatctgt tctggacctc tgacgtgcgc ccgatgacca
    22141 tcgcctggcc gctggaaatc gcccaggatc tgcgtcgtct catttcgctg gacaccttca
    22201 gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacaatt ttgccgctgt
    22261 ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc acccgttttc
    22321 ttgaacgttc ggcggcgaaa gttggcaaag tgactcagga tcacttctgg ctgacgttgc
    22381 gcactctttt ctggtcgatt ctcgtcgcgt caccgttacc ggtgctgtgg atgacgctgg
    22441 gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc gatggtgtaa
    22501 cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg
    22561 gcttgtttat cgctcatttt ggctggccgc gcgaacgcgt atctcgtggg atgcgctact
    22621 acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg ttcgataacc
    22681 tcgacgaccg tgaattctcc ggttcgctgg gaaggctttg ctttatcctc atttgcggtg
    22741 cgctggcggt ggtcaccctc agcctgaaaa aggccgggat tccgctgtat ctcaacaaag
    22801 aaggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt ggcgcgccgt
    22861 tggttgccat tctggcgtcg gctgtgggtt atctggcaac ggcacaggcg ctgttagcga
    22921 ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac gttatccgcc
    22981 gctggatgct gatccagcgc cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag
    23041 agatgttagc gcaacgtgcg cgtggcgaag aggaagcgca tcatcacagt agcccggaag
    23101 gagcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg caatccttgc
    23161 ggctggtgcg ctcaattttg atgttgatcg ccctgctttc tgtcattgtg ctgtggtcag
    23221 aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc acctccacgg
    23281 tacagggcgt agaaagtctg gagccaatta ccctcggtgc ggtgctgatt gccattctgg
    23341 tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa ctggcgattt
    23401 tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc accaaatatc
    23461 tgctgatgct gattggcggg ctggtcggct tctcgatgat tggtattgag tggtcgaaat
    23521 tgcagtggct ggtcgccgcg ctcggtgttg gtctcggttt tggtttgcag gaaattttcg
    23581 ccaactttat ctctggtctg attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg
    23641 tgacaattcg cgatctcacc ggtagcgtga cgaaaattaa cacccgcgcc accaccatca
    23701 gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc gagcagttta
    23761 tcaactggtc gctctctgac tcggtcacgc gcgtagtgtt gacgattccg gcccctgctg
    23821 atgccaacag tgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg
    23881 tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacaaggg attcagattt
    23941 tcgagctgcg tatttatgcc gctgagatgg gtcaccgtat gccgctacgc catgagatcc
    24001 accagctgat tctggctggc ttccatgccc acggtatcga tatgccattc ccgcccttcc
    24061 agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg tctgcgggca
    24121 aaggtcgtca ggcgggaagt ttgtaatgca tgaagctgcc aaatgatgtc aacgtggctc
    24181 atttagcagc ttaacatcat cttcagttat caccggccct ggagagtatc atccatacgc
    24241 attacttcct gacaactcat cgccggagag acgatgagtt gcaggatttc atatgatcag
    24301 tcgagagcta actcaggttt attcgccatg cgactgcaat agttctggta aatcgccatc
    24361 gctaacagcg agaagaagac agggccgcca atcatccaca atgtgctgtc ccagtctccg
    24421 gcttcaacca caggctgaat aatggtgaag acgttcgcaa atgtcaccac cagtacgaca
    24481 accaccatcg caatcattgc cgacatacgc gttttgaaaa tcacaaatgg tctgtcgaga
    24541 tcctgacgtg ctttaaagaa tgggaaagcc agcgcgagga acaggtaagg aagcgtcata
    24601 gacacgttcg ccatcagcgt cagcttgtta aagaacgcgg atgcggtacc gccaccaaac
    24661 gaaaccagca ggatgaagac agtaaccaac ccgcactgca tccacatggc gatagacggc
    24721 atccccatcg cattcaggcg cgtcatcggt tccggccaca acgctttcgg cgtcccctgg
    24781 atgatggctt tcagcggtga atagcaaagc gtaaagaacg caccggtata agcgaggaac
    24841 atcgaaagtc cggtaatacg cgcaaaccat acgcccagcg acaatgacgc ttcaggtgac
    24901 aaatgcagtg cgttacccag cgtcaccccg aggctcttca tcagcacata ggtaatattg
    24961 ccaaggttaa cggaaccatt acttaatacc tgctgccagt ttgtgctgac gccccataaa
    25021 aatattgcca gcgaataacc gattgaaata acaatagcgg caaaaacaat acctttggca
    25081 aagttctttt ctggattttc cgttttatcg accagaccac caaccgcttc aattccgcca
    25141 taggcaaaaa tagcaaatac cacaaacgat agcattgcca gaccggactg ataacccggg
    25201 ttcggtgatg caaggaaatt aatatcctgc gcgaaatgcc caccatttaa taacaaaata
    25261 gtaatgctta ctaacagcaa tactaaattc agacacatta ctgcaatacc gcccaccgca
    25321 gtaatgcggg caattttatt aatccccttt gaagcaacga cggtgaccag aatcatccat
    25381 gctactgcca gcagaccaac cacctgcgta ggctccagtc cggcaatacg ccagtgctgg
    25441 gtcatgtcgc taccatagag gaatgttgag aacggtaccc aaactttcgc ggaggtactc
    25501 accatccaga tgatataaga ggaaaaccac ataaacgtac caatgaaggc aaaacgtggt
    25561 ccgacactat tattcatcca ggaatagata ccaccttctt ctttgcgata agcagctccc
    25621 atttcagcca tcattaa
//
LOCUS       DAZPBM010000044        25284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPBM010000044 DAZPBM010000000
VERSION     DAZPBM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.044"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            17..268
                     /locus_tag="ACHUZQ_001678"
                     /pseudo
     CDS             17..268
                     /locus_tag="ACHUZQ_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="ACHUZQ_001679"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="ACHUZQ_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1025396.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="ACHUZQ_001680"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="ACHUZQ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1025397.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="ACHUZQ_001681"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="ACHUZQ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1025398.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="ACHUZQ_001682"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="ACHUZQ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1025399.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="ACHUZQ_001683"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="ACHUZQ_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1025400.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHUZQ_001684"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHUZQ_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1025401.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="ACHUZQ_001685"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="ACHUZQ_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1025402.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHUZQ_001686"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHUZQ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1025403.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="ACHUZQ_001687"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="ACHUZQ_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1025404.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHUZQ_001688"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHUZQ_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1025405.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHUZQ_001689"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHUZQ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1025406.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="ACHUZQ_001690"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="ACHUZQ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1025407.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="ACHUZQ_001691"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="ACHUZQ_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1025408.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="ACHUZQ_001692"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="ACHUZQ_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1025409.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHUZQ_001693"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHUZQ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1025410.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHUZQ_001694"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHUZQ_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1025411.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="ACHUZQ_001695"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="ACHUZQ_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1025412.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="ACHUZQ_001696"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="ACHUZQ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1025413.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHUZQ_001697"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHUZQ_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1025414.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHUZQ_001698"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHUZQ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1025415.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="ACHUZQ_001699"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="ACHUZQ_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1025416.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="ACHUZQ_001700"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="ACHUZQ_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1025417.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHUZQ_001701"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHUZQ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1025418.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHUZQ_001702"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHUZQ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1025419.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHUZQ_001703"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHUZQ_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1025420.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHUZQ_001704"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHUZQ_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1025421.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHUZQ_001705"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHUZQ_001705"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1025422.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHUZQ_001706"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHUZQ_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1025423.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="ACHUZQ_001707"
     CDS             14267..14635
                     /locus_tag="ACHUZQ_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR1025424.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="ACHUZQ_001708"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="ACHUZQ_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR1025425.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="ACHUZQ_001709"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="ACHUZQ_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR1025426.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHUZQ_001710"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHUZQ_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR1025427.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHUZQ_001711"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHUZQ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1025428.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHUZQ_001712"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHUZQ_001712"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1025429.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHUZQ_001713"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHUZQ_001713"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1025430.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHUZQ_001714"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHUZQ_001714"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1025431.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="ACHUZQ_001715"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="ACHUZQ_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1025432.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /gene="smg"
                     /locus_tag="ACHUZQ_001716"
     CDS             21312..21785
                     /gene="smg"
                     /locus_tag="ACHUZQ_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1025433.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="ACHUZQ_001717"
     CDS             21814..22356
                     /locus_tag="ACHUZQ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1025434.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHUZQ_001718"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHUZQ_001718"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1025435.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="ACHUZQ_001719"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="ACHUZQ_001719"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1025436.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="ACHUZQ_001720"
     CDS             23753..24010
                     /locus_tag="ACHUZQ_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1025437.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="ACHUZQ_001721"
     CDS             complement(23986..24540)
                     /locus_tag="ACHUZQ_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1025438.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25284
                     /locus_tag="ACHUZQ_001722"
     rRNA            25013..>25284
                     /locus_tag="ACHUZQ_001722"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5992 c         6617 g         6233 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga tatcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataatgtcgc
    25201 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
    25261 ttgttggtgg ggtaacggct cacc
//
LOCUS       DAZPBM010000045        24934 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPBM010000045 DAZPBM010000000
VERSION     DAZPBM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.045"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHUZQ_001723"
     CDS             complement(<1..163)
                     /locus_tag="ACHUZQ_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1025439.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUZQ_001724"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUZQ_001724"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1025440.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUZQ_001725"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUZQ_001725"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1025441.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUZQ_001726"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUZQ_001726"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1025442.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUZQ_001727"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUZQ_001727"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1025443.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUZQ_001728"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUZQ_001728"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1025444.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHUZQ_001729"
     CDS             5058..6191
                     /locus_tag="ACHUZQ_001729"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1025445.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHUZQ_001730"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHUZQ_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1025446.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUZQ_001731"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUZQ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1025447.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUZQ_001732"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUZQ_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1025448.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUZQ_001733"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUZQ_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1025449.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUZQ_001734"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUZQ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1025450.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUZQ_001735"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUZQ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1025451.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..10631
                     /locus_tag="ACHUZQ_001736"
     CDS             9762..10631
                     /locus_tag="ACHUZQ_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1025452.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10834..11187)
                     /locus_tag="ACHUZQ_001737"
     CDS             complement(10834..11187)
                     /locus_tag="ACHUZQ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1025453.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHUZQ_001738"
     CDS             complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHUZQ_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1025454.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13015..13308)
                     /locus_tag="ACHUZQ_001739"
     CDS             complement(13015..13308)
                     /locus_tag="ACHUZQ_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1025455.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHUZQ_001740"
     CDS             13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHUZQ_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1025456.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14862..15332)
                     /locus_tag="ACHUZQ_001741"
     CDS             complement(14862..15332)
                     /locus_tag="ACHUZQ_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1025457.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15669..17105
                     /gene="aspA"
                     /locus_tag="ACHUZQ_001742"
     CDS             15669..17105
                     /gene="aspA"
                     /locus_tag="ACHUZQ_001742"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1025458.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHUZQ_001743"
     CDS             17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHUZQ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1025459.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18640..18978
                     /gene="cutA"
                     /locus_tag="ACHUZQ_001744"
     CDS             18640..18978
                     /gene="cutA"
                     /locus_tag="ACHUZQ_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1025460.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHUZQ_001745"
     CDS             18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHUZQ_001745"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1025461.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20688..21263
                     /locus_tag="ACHUZQ_001746"
     CDS             20688..21263
                     /locus_tag="ACHUZQ_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1025462.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21370..21445
                     /locus_tag="ACHUZQ_001747"
     tRNA            21370..21445
                     /locus_tag="ACHUZQ_001747"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21403..21405,aa:Phe,seq:gaa)
     gene            21688..21802
                     /locus_tag="ACHUZQ_001748"
                     /pseudo
     CDS             21688..21802
                     /locus_tag="ACHUZQ_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            22062..22361
                     /locus_tag="ACHUZQ_001749"
     CDS             22062..22361
                     /locus_tag="ACHUZQ_001749"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR1025463.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHUZQ_001750"
     CDS             complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHUZQ_001750"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR1025464.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            22544..23599
                     /gene="cadC"
                     /locus_tag="ACHUZQ_001751"
     CDS             22544..23599
                     /gene="cadC"
                     /locus_tag="ACHUZQ_001751"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR1025465.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            23964..>24934
                     /gene="cadB"
                     /locus_tag="ACHUZQ_001752"
     CDS             23964..>24934
                     /gene="cadB"
                     /locus_tag="ACHUZQ_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1025466.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         6222 a         6267 c         6122 g         6323 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
     9901 tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc
     9961 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc
    10021 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    10081 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    10141 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    10201 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    10261 acgacgttga ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat
    10321 ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    10381 gcaaagaaca atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    10441 tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg
    10501 tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg
    10561 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    10621 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    10681 ataacgtgat cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc
    10741 tcaagcattc aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca
    10801 ataatacgtt tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc
    10861 gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat
    10921 gccataaatc acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc
    10981 gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt
    11041 cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg
    11101 accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac
    11161 aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac
    11221 tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac
    11281 atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc
    11341 atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc
    11401 aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc
    11461 agagcagaac gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat
    11521 tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca
    11581 acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc
    11641 agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta
    11701 gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca
    11761 cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta
    11821 gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt
    11881 ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg
    11941 ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc
    12001 acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc
    12061 tcttcagaga tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga
    12121 cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta
    12181 acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg
    12241 cctgctttgg caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg
    12301 tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg
    12361 aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc
    12421 tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct
    12481 tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca
    12541 atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta
    12601 acggctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc
    12661 agaccctcag tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca
    12721 gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg
    12781 tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg
    12841 aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct
    12901 gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct
    12961 ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc
    13021 ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt
    13081 ctcagatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag
    13141 cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg
    13201 ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac
    13261 ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc
    13321 tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt
    13381 gaccagagaa atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg
    13441 atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat
    13501 cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt
    13561 tgggcgcaat cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
    13621 gcattggcct gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag
    13681 ctgcacaggt agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
    13741 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg
    13801 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt
    13861 tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg
    13921 caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg
    13981 tgtggtatat cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg
    14041 gacttatcgt cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata
    14101 tccccttccc ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga
    14161 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
    14221 cagagcgtga ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact
    14281 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag
    14341 catcgcttcc aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg
    14401 taattggcta tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
    14461 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
    14521 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga
    14581 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
    14641 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
    14701 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
    14761 tctatgcgct gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
    14821 aaaatggcat aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga
    14881 tcaaggcggt cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
    14941 ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac
    15001 ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg
    15061 aaaaagcccg gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc
    15121 tccgccgcag ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
    15181 ccctggttac gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc
    15241 agcaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
    15301 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta
    15361 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
    15421 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
    15481 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
    15541 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
    15601 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
    15661 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc
    15721 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
    15781 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
    15841 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
    15901 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
    15961 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
    16021 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
    16081 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
    16141 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
    16201 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
    16261 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca
    16321 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
    16381 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg
    16441 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
    16501 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
    16561 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
    16621 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
    16681 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
    16741 agctgcagtt gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca
    16801 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
    16861 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
    16921 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
    16981 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
    17041 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
    17101 agtaatcgta cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt
    17161 gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta
    17221 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
    17281 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
    17341 ttaaacccgg taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta
    17401 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
    17461 tgcgccgtaa cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa
    17521 tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga
    17581 aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
    17641 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
    17701 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
    17761 tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt
    17821 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
    17881 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
    17941 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
    18001 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
    18061 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
    18121 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
    18181 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
    18241 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg
    18301 cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
    18361 ttctgccgac ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc
    18421 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
    18481 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc
    18541 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga
    18601 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
    18661 aataccgcgt ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta
    18721 gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
    18781 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
    18841 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
    18901 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
    18961 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
    19021 acgcgccggg acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc
    19081 aaaaccaaca tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta
    19141 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
    19201 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
    19261 ccgtaaccat caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
    19321 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
    19381 ccaacaacgc agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat
    19441 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
    19501 tgctgccaat gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca
    19561 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg
    19621 cgctgggtct ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
    19681 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    19741 ttactctaca actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac
    19801 agggcggctc acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac
    19861 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    19921 ggctgggcgg cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    19981 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    20041 ccgcgtttgg ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg
    20101 atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    20161 tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    20221 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    20281 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    20341 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    20401 gtaaaaagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    20461 cggtcttact tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc
    20521 atcttaatgt ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc
    20581 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    20641 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    20701 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    20761 agatggttgc tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca
    20821 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    20881 gtcagctgat gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    20941 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    21001 cgttttatcc cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg
    21061 cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag
    21121 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc
    21181 aggcggatgt cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    21241 gccagggtgg tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    21301 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    21361 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt
    21421 ggttcgattc cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt
    21481 ttcgtttcag gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa
    21541 ccagattctc cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt
    21601 cctgagcgat agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt
    21661 caaatgacgt catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga
    21721 ctgatgagat tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa
    21781 gactaacaat ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac
    21841 acatttttct ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc
    21901 agtaaaatcc tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc
    21961 ttgcattcac caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc
    22021 aattatcatt gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc
    22081 gcgttggcga atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac
    22141 ttacccttga gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag
    22201 tacttagcag ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg
    22261 ttgtgacgca gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc
    22321 ctgtctatat cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt
    22381 acagcgaaga agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg
    22441 ttcctgacac aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg
    22501 aacaatcccc agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt
    22561 gtcgttaggt atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat
    22621 gagcaaatcg cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag
    22681 ttgtaacagc tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt
    22741 ggcgacatca cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat
    22801 tgatgaaccg agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata
    22861 ccgtgctcaa caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat
    22921 gctggataaa cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact
    22981 ggagagttta tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca
    23041 gcaaattttg cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt
    23101 actgcatggc gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca
    23161 atcatcccca gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca
    23221 ttctcaacac cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat
    23281 tgttacactg ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc
    23341 tctggtaaaa ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga
    23401 aatgtcctgg ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg
    23461 ggaagcagct gatgcatatc tcactgcctt taatttacgc ccaggggcaa acacccttta
    23521 ctggattgaa aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa
    23581 atttctcgct tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg
    23641 agtttttgta tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt
    23701 tattacataa atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc
    23761 ttttcatgat atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta
    23821 cagaaacttt tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc
    23881 ttatcgccag tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc
    23941 aaaaatgaaa ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta
    24001 ccggtgttgt tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag
    24061 caagtatcgg tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc
    24121 tggcgtatgt atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt
    24181 atgccggaga aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta
    24241 actggattgg taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc
    24301 cagtattaaa tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta
    24361 cctttgtaaa tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc
    24421 tggttcttat tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa
    24481 cttatgcagc taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta
    24541 ttctgctcta cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg
    24601 ttaaaaaccc gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta
    24661 ttgtttacat cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg
    24721 cttccggtgc tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc
    24781 tggtttctgc attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg
    24841 taggccaggc aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag
    24901 tcgacagcaa cggtattccg aaaaaaggtc tgct
//
LOCUS       DAZPBM010000046        24817 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPBM010000046 DAZPBM010000000
VERSION     DAZPBM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24817
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.046"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(88..378)
                     /gene="ubiK"
                     /locus_tag="ACHUZQ_001753"
     CDS             complement(88..378)
                     /gene="ubiK"
                     /locus_tag="ACHUZQ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1025467.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     regulatory      500..648
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            752..1405
                     /gene="ribB"
                     /locus_tag="ACHUZQ_001754"
     CDS             752..1405
                     /gene="ribB"
                     /locus_tag="ACHUZQ_001754"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1025468.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1666..1836
                     /gene="yqiD"
                     /locus_tag="ACHUZQ_001755"
     CDS             1666..1836
                     /gene="yqiD"
                     /locus_tag="ACHUZQ_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1025469.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1894..2667)
                     /gene="zupT"
                     /locus_tag="ACHUZQ_001756"
     CDS             complement(1894..2667)
                     /gene="zupT"
                     /locus_tag="ACHUZQ_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1025470.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2810..3598
                     /gene="ygiD"
                     /locus_tag="ACHUZQ_001757"
     CDS             2810..3598
                     /gene="ygiD"
                     /locus_tag="ACHUZQ_001757"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1025471.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3636..4796)
                     /locus_tag="ACHUZQ_001758"
     CDS             complement(3636..4796)
                     /locus_tag="ACHUZQ_001758"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1025472.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(4802..5473)
                     /locus_tag="ACHUZQ_001759"
     CDS             complement(4802..5473)
                     /locus_tag="ACHUZQ_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1025473.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5621..7102)
                     /gene="tolC"
                     /locus_tag="ACHUZQ_001760"
     CDS             complement(5621..7102)
                     /gene="tolC"
                     /locus_tag="ACHUZQ_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1025474.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7307..7936
                     /gene="nudF"
                     /locus_tag="ACHUZQ_001761"
     CDS             7307..7936
                     /gene="nudF"
                     /locus_tag="ACHUZQ_001761"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1025475.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7937..8359
                     /locus_tag="ACHUZQ_001762"
     CDS             7937..8359
                     /locus_tag="ACHUZQ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1025476.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            8384..9211
                     /gene="cpdA"
                     /locus_tag="ACHUZQ_001763"
     CDS             8384..9211
                     /gene="cpdA"
                     /locus_tag="ACHUZQ_001763"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1025477.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9211..9792
                     /gene="yqiA"
                     /locus_tag="ACHUZQ_001764"
     CDS             9211..9792
                     /gene="yqiA"
                     /locus_tag="ACHUZQ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1025478.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9821..11713
                     /gene="parE"
                     /locus_tag="ACHUZQ_001765"
     CDS             9821..11713
                     /gene="parE"
                     /locus_tag="ACHUZQ_001765"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1025479.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11761..12075)
                     /locus_tag="ACHUZQ_001766"
     CDS             complement(11761..12075)
                     /locus_tag="ACHUZQ_001766"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1025480.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12106..12687)
                     /gene="mdaB"
                     /locus_tag="ACHUZQ_001767"
     CDS             complement(12106..12687)
                     /gene="mdaB"
                     /locus_tag="ACHUZQ_001767"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1025481.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(12797..14146)
                     /gene="qseC"
                     /locus_tag="ACHUZQ_001768"
     CDS             complement(12797..14146)
                     /gene="qseC"
                     /locus_tag="ACHUZQ_001768"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1025482.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(14143..14802)
                     /gene="qseB"
                     /locus_tag="ACHUZQ_001769"
     CDS             complement(14143..14802)
                     /gene="qseB"
                     /locus_tag="ACHUZQ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1025483.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            14954..15346
                     /gene="ygiW"
                     /locus_tag="ACHUZQ_001770"
     CDS             14954..15346
                     /gene="ygiW"
                     /locus_tag="ACHUZQ_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1025484.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15399..15881
                     /locus_tag="ACHUZQ_001771"
     CDS             15399..15881
                     /locus_tag="ACHUZQ_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1025485.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            16427..18685
                     /gene="parC"
                     /locus_tag="ACHUZQ_001772"
     CDS             16427..18685
                     /gene="parC"
                     /locus_tag="ACHUZQ_001772"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1025486.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            18918..19655
                     /gene="plsC"
                     /locus_tag="ACHUZQ_001773"
                     /gene_synonym="parF"
     CDS             18918..19655
                     /gene="plsC"
                     /locus_tag="ACHUZQ_001773"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1025487.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            19730..21142
                     /gene="ftsP"
                     /locus_tag="ACHUZQ_001774"
     CDS             19730..21142
                     /gene="ftsP"
                     /locus_tag="ACHUZQ_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1025488.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21253..23472
                     /locus_tag="ACHUZQ_001775"
     CDS             21253..23472
                     /locus_tag="ACHUZQ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1025489.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(23515..23772)
                     /gene="yqhH"
                     /locus_tag="ACHUZQ_001776"
     CDS             complement(23515..23772)
                     /gene="yqhH"
                     /locus_tag="ACHUZQ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1025490.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(23823..24749)
                     /locus_tag="ACHUZQ_001777"
     CDS             complement(23823..24749)
                     /locus_tag="ACHUZQ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1025491.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
BASE COUNT         5802 a         6235 c         6643 g         6137 t
ORIGIN      
        1 tctgaatgga attaagcgat aaggtaatag aaagcactgt aaattagaag attaattcat
       61 aatttacagt gcattaaaga caaatgttta cagcgttggg gggagagtct ctggatctgg
      121 ctgcttttta atctcagtac tgcgattttc cagttcgctc attctttgtt caagccgcgc
      181 cagtttctca cgagtccgta acaggacttg cgtttggacg tcgaactctt cacggcttac
      241 aaggtcgagg cgcgtcagct gagcttgtag ggtttggcgg atttttttct ccacatcttc
      301 cccgaactcc ctgattcctt taggcattga ttcgtgaacc tggcgagcga tttgctcaat
      361 ttttttcggg tcaatcattg tagtttccct gtactgatag gtgttgagta ccattgtagt
      421 gcgataaggg taagtcataa accagaatta tgtgaagcta tgcgttgctg ccgctaatca
      481 ttagcgttat agtgaatccg cttattctca gggcggggcg aaattcccca ccggcggtaa
      541 atcaactcag ttgaaagccc gcgagcgctt tgggtgcgaa ctcaaaggac agcagatccg
      601 gtgtaattcc ggggccgacg gttagagtcc ggatgggaga gagtaacgat tctgtcgggc
      661 atggacccgc tcacgttatt ttggctatat gccgccactc ctaagactgc cctgattctg
      721 gtaaccataa ttttagtgag gtttttttac catgaatcag acgctacttt cctcttttgg
      781 tacgcctttc gaacgtgttg aaaatgcact ggctgcgctg cgtgaaggac gcggtgtaat
      841 ggtgcttgat gatgaagacc gtgaaaacga aggcgatatg atcttcccgg cagaaaccat
      901 gaccgttgag cagatggcgc tgaccattcg ccacggtagc ggtattgttt gcctgtgcat
      961 cactgaagat cgccgtaaac aacttgatct gccaatgatg gtagaaaata acaccagcgc
     1021 ctatggcacc ggttttaccg tgaccattga agcggctgaa ggtgtgacta ccggtgtttc
     1081 tgccgctgat cgtattacga ccgttcgcgc agcgattgcc gatggcgcaa aaccttctga
     1141 tctgaatcgc ccgggccacg ttttcccact tcgcgcgcag gcaggtggtg tactgacgcg
     1201 tggcggtcat actgaagcga ctattgattt gatgacgctg gcaggcttta aaccggctgg
     1261 tgtgctgtgt gagctgacta atgacgatgg cacgatggcg cgtgcaccag agtgcattga
     1321 gtttgccaat aaacacaata tggcgctcgt gactattgaa gacctggtgg cataccgtca
     1381 ggcacatgag cgtaaagcca gctgaaaacc gctgcttaat tactgcctta atcaagaaac
     1441 cgaagttgta gctggcttcg gtttttattt tgccctgcta tgcaagatgt taattccgct
     1501 tctctttcta tgagaaaatt tcattaatat caggcattct ttttcattat gatcaaaatc
     1561 atataattgt tccgttatta tgcgaccata tgaatatatc cgaaaaagga tatgtgaaat
     1621 ttatgcgcta aatgtttagt attgttaaat attaagggta aaaggatgtt tatcgcctgg
     1681 tactggattg tattgattgc tctggttgtg gtgggttatt tcctgcattt gaaacgttat
     1741 tgtcgggcgt ttcgccagga cagagacgca ctgcttgaag cgcggaacaa atacttaaac
     1801 agtacgagag aagagacagc cgaaaaggta gaataaggct ttcgtgaagc tggcagaagt
     1861 tgtccatgtg ctggacaact tctgatgttg tttttaacca attcccgccg tttgtagcag
     1921 cactaaactg aatcccatca ctgacattcc acatagtacg ccatagctgg ggttattatt
     1981 agggtcaatc tctttggcga gcggcattaa ttcatcaacc gagagcgcca ccataattcc
     2041 tgcaaccgcc gccatgattg ccgccatgac caccggggaa atcatgctac cgaggattaa
     2101 ccacgccagc acaccaccaa gaatttctgc tagtccagaa atccccgccc acagaatcgc
     2161 ggtgcgttta gaccccgttg ccgcataaac cgggcctgcc actgccagac cttcagggat
     2221 attgtgcaac gcgacggcca gtgcgatgcc aaatcccagc tccaggttgc tgctcgccgt
     2281 gacaaaggtg gcaatccctt ccgggaagtt atgcagactg atgccgagag tgagcagaat
     2341 ggctgtgcgc ttgatcgatt ttggcaacgg ctgcaccgat ttttgcatta aatcctgcgg
     2401 atgcgcatgt ggcaacatgc ggtccaggcc aaaatagcca agcagaccga agataaacat
     2461 tccataaccc aacacaggcg acattccttc agctgctagt gcggcaggaa gcatttccat
     2521 taatgagatg agcaacatga tccccgccgc aaatcctagc gaaaacgcca gtaagcggtt
     2581 cgagggtttt tgcccgagaa cgccgagaaa cgcgccaata aacgtggctg cccccgccag
     2641 tatggtcaga atgagaggta ctgacatcga caactcctta taaatcttca ccttcaggca
     2701 gatcgtcaaa ataactgatg atcatcatca ttgttatccg ggttcttact ccgccgaaca
     2761 agcgtattgt gaggatatca aaatgacacc tttagttaag gatatcatca tgtcttcaac
     2821 acgtatgcca gcattgtttt taggtcacgg tagtccgatg aacgtgctgg aagataattt
     2881 gtatacccgc agctggcaga agttggggat gacattgcca cgcccgcaag cgattgtggt
     2941 ggtttcggct cactggttta cccgtggaac aggagtgacc gcgatggaga cgccgcccac
     3001 gattcatgac tttggtggct tcccgcaggc gctgtacgat acgcattatc ctgctccggg
     3061 ttcgcctgtg ctggcacagc atctggttga gctgttagcg tcggtcccgg tgacgctgga
     3121 taaagaagcc tggggctttg accacggttc ctggggcgtg ctgattaaga tgtatcctga
     3181 cgccgatatc ccgatggtgc agttgagtat cgacagtagc aaacctgccg cctggcattt
     3241 cgaaatgggg cgcaaactgg cagcgctgcg agatgaagga ataatgttgg tcgccagtgg
     3301 caacgtggtg cataacctgc gtacagtgaa gtggcacggt gatagttcac catatccttg
     3361 ggcgatgtcg tttaatgagt atgtgaaagc gaatctgacg tggcaagggg cggtagagca
     3421 gcatcctctg gtgaattacc ttgaccatga aggtggcgcg ttatcgaacc caacgccgga
     3481 gcactatctg ccgttgttgt atgtcttagg tgcgtgggat gggcaggagc caattaccat
     3541 tccggtcgat ggtatagaaa tgggcagcct gagtatgctg tcggtgcaga taggttaaca
     3601 attaccggat ggcgaatgcc atccggcatc attgattact caataaaaat atgtggataa
     3661 aaccgagaca tatcctgggt gatcaacgca cggtcttcac gaatgccaat tccggcaggt
     3721 tgatcgttca ccagccagct accaatcagc atatagctgt cgccgaattt cggtaacggg
     3781 tggaattgct gaacaatcat cccttcttcg ccatacggac cttccgctgc ttcaatggtt
     3841 ttgccgttct caatgatcga cacgtttgcg ccttcacggg agaagatcgg tttaaccaca
     3901 tatttttcca tttgcggatg atcatcttcc gcaaaataag cgggcagcag gttcgggtga
     3961 ttcgggaaca tctcccacag tagcggtaga agcgctttgt tggagataat gctcttccac
     4021 gccggttcca gccagcgtac gcctgcatcc tccagcttgg tggagaacat ctcacgcaac
     4081 ataaattccc atggatacag cttgaacagg ttggaaatta cctgatcctg taaatccgtg
     4141 aactgacctt tttcacctaa cccgatatcg tcaatgtaga ggaactcggt agcgatttca
     4201 gcttccgtcg cgcagtcctg caaatactga atggttccgc gatcttccac cgtgtcgcga
     4261 cagcaggtga gatgcagcaa ctggaagcca tactgttcac gcaactcgac aaagcgatcg
     4321 attagctttt cttgcagact gttaaactgg tcgctgccct ccggcaagtt accggcgtta
     4381 agctgatctt ccagccagat ccactgaaag aacgccgcct cgtatagtga cgttggtgta
     4441 tcggcgttat tttccagcag tttaggttcg ccagtgccat cccatgccag atcaagacgc
     4501 gaataaagcg atggctggtg agtcagccat gactgacgca caaaactcca ggtgcgtttt
     4561 ggaatgcgga atttggtcat cagctcatcg ctggcgatca ctttttccac cactttcagg
     4621 cacatctggt gcagttcggc ggtgacttct tccagctttt caacctgggc gagggtcaac
     4681 ttgtagtaag catcttcaca ccagtacggc tcgccgtaca tggtgtgaaa attgaaaccg
     4741 tattcgtggg ctttttcgcg ccagtccggg cgctcggtaa tactgactct ttccatcggt
     4801 atcagccacc cattgaacga gaagaggtgc cggttgcgct acgctgcata gtgctttgtt
     4861 tggcaacaga ttcaccaaaa ccgccacggg taacggtagt agtggtcgcc ggttttggtg
     4921 ccatggctgt cttcggtacg gtcatggtgc ggcctggctg ggctgcgcca tagtttttac
     4981 ccgtcgcgtc ggtatattta ccgtaagccg ggctggctgg gtttttcgag gagaacagcg
     5041 gctgctgtgc aaatcccgcg ccgccgccca tcagacgccc catcatgtaa ccggccatca
     5101 gcggcatcca gaaactcccg ctggattgct gggcctgcgc ctggttttct ggtgccatgc
     5161 cagcctgggc tggtgcctgc tggcactgac cttcaccaaa ttcagcaaca cagtcttcac
     5221 gggtggcgta tttcggcgca gtacgttcgg cttctttcag cgcattgttg tacgcggtgg
     5281 tacattcggc gcttttgcct gggtttgcag ctgaacagtc gtcagcattt tgatagagag
     5341 acactgtttc atcactcttt tcacagccag ccagcataaa aacagtggca accgcgagag
     5401 cgactggtgt cagatggcgt gcgctccagt ttttgcggaa cgatgcgtgg cgtatggatt
     5461 ttgtccgttt catttttgtc ttccgggacc agtggtaaat acccatcaga atagaggatg
     5521 gctggtcgaa attgaagcga gaagggggaa gaatgcggca gataacccgt atctttacgt
     5581 tgccttacgt tcagacgggg ccgaagcccc gtcgtcgtcg tcagttacgg aaagggttat
     5641 gaccgttact ggtggtagtg cgtgcggatg tttgctgaac gactggtgcc gggctatcag
     5701 gcgcataacc atcagcaata gcattctgtt ccggcgtttg cggtgcaacg ttttccggat
     5761 tagtggaaac cggtttgctc agcgcattgt tcagtgccag cagatcctgc tcgttcaacg
     5821 tacccagagc tgacttaata ttcaactggt taatcaggta gttataacgc gcatttgcca
     5881 gctcttgctt agcgttgtac agcgtggtgg ttgcatccaa cacatcaaca atggtacgcg
     5941 taccgaccga gtagcccgct tccatcgcgt ctaatgagct ttgagcggaa actacggctt
     6001 gtttgtaggc gttaatgcta ctgatagatg cattaatgtt gttgaaggag gaacgtacgg
     6061 tttgcacgac gctacgatgc gcgctttcca gttgctcgct ggcaccaaca aagttgtact
     6121 gggcctgttt cacctgcgag ttaaccattc cgccctgata aatcggcagc gagaagctca
     6181 ggcccacttt gttctggccc atattgctgt cgtcatactg ggtaccagcg gcaccacggg
     6241 ttttcgaacc gctataagag gtgtcagaaa tcccggtaga agccgttaaa tccagcgtcg
     6301 gtaagtgacc atcctgcgcc tggcgaattt gctcgcgcgc caggtcctgg ctcaagcgtg
     6361 cctgtaacag cgacaggttg cgtttttcgg cttctttcag cagcgcgtta accggctgtg
     6421 gtttgtcggt tttaaagttt tcgacattca gcgccgccag ttccggatag tagttaccgg
     6481 tgatctggcg cagctgctct accgcgttat caaggttatt acgtgcggtc acttcgttcg
     6541 ccagcacggt atcgtactgc gcgcgggcgt tctgcacgtc ggtgatcgct accaggccca
     6601 cgttaaaacg ttgggtggtt tgatctaatt gacggtagat cgcttctttt tgtgcctgtg
     6661 tataggaaag aacgtcaata gcattcaaca cgttgaaata agcggtcgcg gtgttgagga
     6721 tcaaggtttg ctgatcggtc tgatatgtga cgtcctgaat ccctgctgct ttttcctgca
     6781 gcgttaacgc acgccatttc gacatatcaa aaatggattg agttaactgc agggacgcac
     6841 tggtcgcgtt agagttgatg ccgttcgcgt cgcggtagcc gttgctatag gtgtaatctg
     6901 cacctaaacc tagctgtggc agtaatggac tgcgcgcttc attaattttt tcaaaggcag
     6961 catcacgatc ggcggcagac ttacgcaatt ccgggttact aaggcgtgct tgctgataaa
     7021 cttgcatcag gttctcggcc tggctcaacg aactgaaccc agaaaggctc aggccgataa
     7081 gaatggggag caatttcttc atttgcattc cttgtggtga agcagtattt agcgcgatca
     7141 aactgtaaaa ttattgcgga ttctagcaga agccgctacc gcaaaaggtt ggcgttacgt
     7201 gccatagggc gttaatgtgc ctcaatttaa cacatgactg ctcaaacggc agtcaaacgt
     7261 cgctgaaatt cacatttaat tcactattag tgccaggaca ttaacaatgc ttaagccaga
     7321 caacctgccc gttacatttg gcaaaaacga tgtagaaatt attgcacgag aaacacttta
     7381 tcgcggcttt ttttcattag atctttatag atttcgtcat cgtctattca acgggcaaat
     7441 gagtcatgag gtacggcggg aaatttttga gcgcggtcac gccgcagtct tactaccctt
     7501 tgacccagtg cgcgatgaag ttgtgctgat tgagcagatt cggattgccg catacgacac
     7561 cagtgaaacc ccctggctac tggagatggt tgccgggatg atagaagagg gtgaaagtgt
     7621 ggaagatgtt gcccgtcgcg aagcgattga agaggcggga ctgatagtca aacggaccaa
     7681 accggtgtta agtttcctgg caagcccggg gggcaccagt gagcgttcgt caattatggt
     7741 gggcgaagtg gacgccacga ccgcaagcgg tattcatggt ctggctgatg aaaacgaaga
     7801 tattcgcgtt catgtggtaa gccgggaaca ggcataccag tgggtagaag aggggaaaat
     7861 cgacaacgca gcgtcggtca tcgctttgca atggctgcag ctgcatcatc aagcgttaaa
     7921 aaatgagtgg gcataaatga agcgttacac acctgacttt cctgaaatga tgcgcctgtg
     7981 cgagatgaac ttttcacaat tgcgccgttt gttaccgcgc aatgacgcac ccggcgaaac
     8041 tgtaagctat caggtggcaa acgcacaata tcggctgacg attgtggaat cgacccgata
     8101 cactaccctg gtgacaatag aacagactgc gcccgcgatc agttactgga gccttccgtc
     8161 aatgacggtg cgtctgtatc atgacgcgat ggtggctgaa gtgtgttcaa gccagcagat
     8221 ttttcgcttc aaagcgcggt atgattatcc taataaaaag ttgcatcaac gcgacgaaaa
     8281 gcatcaaatt aatcagtttt tagcggactg gttgcgatac tgtttagcac atggagcgat
     8341 ggcgattccg gtttactagc atcgtgaaac ctaaggacac catttggaaa gcctgttaac
     8401 ccttcctctg gctggtgagg ccagagtcag gattttacaa attaccgaca ctcacctgtt
     8461 tgcacaaaag cacgaagctc tgttaggggt aaacacctgg gaaagctacc aggcggtgct
     8521 ggaggcgatt cgtccacatc agcacgaatt cgacctgatt gtcgcgacag gtgatttagc
     8581 gcaggatcaa tcctctgcgg cctatcagca tttcgctgaa ggcatcgcaa gttttcgtgc
     8641 gccctgcgtc tggctgccgg gcaaccacga tttccagccc gcgatgtaca gcgcgttaca
     8701 ggatgcgggt atctccccgg cgaagcgggt gtttattggt gagcaatggc aaatcctgtt
     8761 gctggatagc caggtgtttg gcgtgccgca cggtgagctg agcgagtttc agcttgagtg
     8821 gctggaacgt aaactggccg atgcgccaga acgccatacg ttgctgctgc tgcatcatca
     8881 tccgctacct gcgggttgta gttggctcga tcaacacagt ctgcgtaacg cgggcgaact
     8941 ggataccgtg ctggcgaagt ttccgcacgt caaatacttg ctgtgcggtc atattcatca
     9001 ggagctggat ctcgactgga acggtcgccg cctgctggca acgccgtcga cctgtgtgca
     9061 gtttaagccg cactgttcca actttacgct ggatactatc gcgcccggct ggcgtactct
     9121 cgagttacat gctgatggca cgctgaccac cgaggtgcat cgcctggcgg acacacgttt
     9181 ccaacctgat accgcttcag aaggctactg atgtctacgc ttctttattt acacggtttc
     9241 aacagctcgc cgcgctctgc aaaagcgagc ttgttaaaaa actggctggc ggaacatcac
     9301 cctgacgttg agatgatcat tccgcagttg ccgccgtatc cttccgacgc ggcagaactg
     9361 ctggaatcca ttgtcctgga acatggcggc gattcgctgg gtattgtcgg ttcgtcactg
     9421 gggggatatt acgccacctg gttgtcacaa tgttttatgc tgcccgcagt ggtggtaaac
     9481 ccggcggtgc gcccgtttga actgctgacg gactatctcg gtcagaacga gaacccatac
     9541 accgggcagc aatatgtgct agagtcacgc catatttacg atctcaaagt catgcagatt
     9601 gacccgctgg aagcgccgga tttgatctgg ctgctgcaac agacgggaga tgaagtgctg
     9661 gattaccgcc aggcggtggc gtactacgct tcctgccgcc agactgtcat agaaggcggc
     9721 aaccacgcat tcacgggctt cgaagattat ttcaacccga tcgtcgattt tcttggtctg
     9781 caccatctct gacgatcaac tcgaattgct aacttaaacc atgacgcaaa cttataacgc
     9841 tgatgccatt gaggtactca ccgggcttga gccggttcgc cgccgtccgg ggatgtatac
     9901 cgataccact cgccctaacc atttggggca agaagttatt gataacagtg tcgatgaagc
     9961 actggcgggt cacgcaaaac gcgtggatgt aatcttacat gctgaccagt cgttagaagt
    10021 tattgacgat gggcgcggga tgccggtgga tattcacccg gaagagggtg tcccggcggt
    10081 tgaattgatt ctttgccgtc tgcatgcagg cggtaaattc tctaacaaaa attaccagtt
    10141 ctctggcggc ctgcatggcg tggggatttc ggtggttaac gccctgtcga agcgcgttga
    10201 agttaacgtg cgccgcgatg gtcaggttta taacatcgcc tttgaaaatg gcgaaaaggt
    10261 gcaggattta caggttgtcg gcacttgcgg taaacgcaat accggtacca gcgtgcactt
    10321 ctggccggat gaaaccttct ttgacagccc gcgattttct gtttcacgcc tgacgcatgt
    10381 gctgaaagcc aaagcggtac tgtgccccgg cgttgagatt acctttaaag atgagatcaa
    10441 caacaccgaa cagcgctggt gctatcagga cggtctgaat gattacctgg cggaagcggt
    10501 aaacggttta ccgacgctgc cagaaaaacc gtttatcggt aatttcgctg gcgatactga
    10561 agctgtggac tgggcgctac tgtggctgcc ggaaggcggt gaactgctga ccgaaagcta
    10621 cgtcaacctg atcccaacga tgcagggcgg tacccatgtt aatggcctgc gtcagggcct
    10681 gttggacgcg atgcgtgagt tctgtgaata tcgcaacatt ctgccgcgcg gcgtgaagct
    10741 gtcggcggaa gatatctggg atcgctgcgc ctatgttctg tcggtgaaaa tgcaggatcc
    10801 gcagtttgcc gggcagacga aagagcgtct ctcttcgcgt caatgcgcgg cattcgtttc
    10861 tggcgtggtg aaagatgcct ttatcctgtg gctgaaccag aacgttcagg cggcggagtt
    10921 gctggcagag atggcgattt ccagcgccca gcgtcgtatg cgtgcggcta aaaaagtggt
    10981 gcgtaaaaag ctgaccagcg gcccggcgct gcctggcaaa ctggctgatt gtaccgcgca
    11041 ggaccttaac cgtaccgaac tgttcctcgt ggaaggtgac tccgcaggcg gatctgccaa
    11101 gcaggcgcgc gatcgtgaat atcaggcgat catgccgctg aaaggtaaga tccttaacac
    11161 ctgggaagtt tcttccgacg aagtgctggc ctcgcaggaa gtgcatgata tttcggtagc
    11221 gatcggtatc gatcctgaca gcgacgatct gagccagctt cgttatggca aaatctgtat
    11281 cctcgcggat gcggactctg atggtctgca cattgccacg ctgctctgcg ctttgttcgt
    11341 aaaacacttc cgcgcgttgg tgaaacacgg tcacgtttac gtcgcactgc caccgctcta
    11401 ccgtattgat ctcgggaaag aggtttatta cgcgctgacg gaagaagaga aagagggcgt
    11461 acttgagcaa ttaaaacgca agaaaggcaa gccgaacgtc cagcgtttta aaggtctggg
    11521 ggaaatgaac ccgatgcaat tgcgtgaaac cacgcttgat ccgaacactc gccgtctggt
    11581 gcagttgact atcgatgatg aagacgatca gcgtactgac gcgatgatgg atatgctgct
    11641 ggcgaagaaa cgctcggaag atcgccgcaa ctggttgcaa gagaaaggcg acatggcgga
    11701 gattgaggtt taaaggaaag aacattgccc ggtcaggatg gccgggcaaa caaggcagga
    11761 ttaaatccct ggctgcagaa tacgaatatt catctccagc acgtcgcctt ttacggcttc
    11821 gctgtacgcc ttcatgtgcg gggtttgcag atgcgcttca aggtgcgcga tgctttccca
    11881 ctgctcaatc atcacgatag aatccggtgc catagactgg aaactcacgc cagcagcgca
    11941 atccaccatt ggcgcatagc cgtggcaacc ttcttctttc agtacggttg gaacgatttt
    12001 agcaaactga tccaataccg cctgacggtg atgttgacca ggacgagtac ggatttctgc
    12061 gattacggta agcatggtta actccttcta aagcctgagc tctagttaac caaaaatttc
    12121 cacaagatgc ttgcgatatt cttcagtata gcggggaaca tcaggcattt ttatcacgtc
    12181 attagcgata aatgtcggca gcggttccat gccaaggaat tggtttgctt tatggaacgg
    12241 cagatacaca ccgtcgacgc caacgccgtg gaagaattga tctttttcgg tgaaggcttc
    12301 cattggtgcg ttccaggtca gagaaagcat atattttttg ccctgtacca ggccgccgga
    12361 accgtatttt tttgacggat ctttgcgggt acggccatcg ctggcataca gcgtcccgtg
    12421 accttcggtg aatacatcat caatgtattt tttcactgtc cacggcgcgc ccatccacca
    12481 gcctggcatc tgccagatca ccacatcagc ccagagaaag ttttgtactt ccgctttgac
    12541 atcgtagtcg ctgtcggcgc gaacgatgcg aacatcatgc ccgaggtcgc gcagtgtgcc
    12601 atccgcgact tcggtcaggg tgtcgttcag ttgaccattg gagtgggcga attttttcgc
    12661 gccgttgata atcagggtgt tgctcatttt ttaacctcaa ggtgagacgt ttgtcgacta
    12721 tagtacgtca cagagcggta cggtaaaatt agcaaaatgt gcaaagtctt ttgcgaattt
    12781 agcaaaagtc ttacccttac cagctcacct tcgcctcaaa tccaccttgt tccgcattcc
    12841 caaattcaac attcatgcca tgcaatttgg cgattcgctg gacaatcgat agcccaagtc
    12901 cactgccggt agcggtttgt ccgggggggc gatagaagcg ttcgccaatt cgcgccagtg
    12961 cctctggtgt cacaccgggg ccgttatccc tcacgatgaa attatcggca ttcagcgtga
    13021 cgtccaccac gctgccctgt ggactgtagc gcacggcgtt atccagaagg tttcgcacca
    13081 acaaacttag caatagcggt tgtccagtgc gtttgatgct gtgggcattg agtgtcagtc
    13141 gcacgtcaat tttcgcctgc tgcgccgtgt ggtaaatatc catcaccgac gattgcagga
    13201 gatcttcaag cgggatctcc gcgacgtcct gaaggttatc cagtgagtcc agccgcgata
    13261 gcgtgagcag ttgatcaacc agacgagtag cgcgatcgat cccggaatgt aattggagca
    13321 gtgctttttt ccgcgcctgc ggatcatcgt cagagagctg cgcaacttcg gtttgcactt
    13381 tcagcgctgt taacgggcta cgtagttcgt gagctgcatc ggaggtaaag cgtcgttctc
    13441 gaaccatcat cgcatgtgtg cgggcgaaca gttgatttag cgactcaacc agtggtcgca
    13501 cttcgctggg tacgccagtc gcgtttagtg gtttttccga gtcagggtca cgcatacgta
    13561 gtgccagcgc cagtttgttc agcggcgcga gttcacgacc cagtagtacc atcatgatga
    13621 ttaacataat cggcagtgcg accagccacg ggatcaattg cccggcaaca atcgccagcg
    13681 ccatgtcttc acggtattcc cattcctggc caacaacgat gcgatatttg ccatcaggtg
    13741 aggtcatcca gacaaaacgc caaggatcgt cttcaccgac cagttgcccg tcagcaaaac
    13801 cttcccgttg atagctatag ggaatatctt ctccgttatc gccatcatta aggaccattc
    13861 tgccgtcgtg ggtaaagatg gcaaaggtca gcgcatcgtc atcaacatga ccgtgtttta
    13921 atttatttgg cgtctgtgcc atgcgatccg ccgcgttgat ttcgttgaga tcgagcgtac
    13981 ttaaccgctt ggcaaacagc atcagttggg tgtcgaacaa ttcatcgacg ttatccgttg
    14041 tttgtttcca ggcgacaaag ctggaaagca gccaggtcac cgaggccaga attaaaaaga
    14101 ttagcgtcag cctgacgcgc agactaagac gttgggtaaa tttcatttct cacctaatgt
    14161 gtagccaata ccatgcacgg tacgaataaa atcactaccg agtttgcgtc gcagatgatg
    14221 cacatgcact tcaacggcat tactggtgac ctcttcgtcc caggtataca gtttctcttc
    14281 aatcagtttg cgcggcagta cccgaccagc gttacgcatc agtaattcca gcagggcaaa
    14341 ttcttttggt ttcagtgtta agggttcgcc agccagcgtg gcgatacgtt tgccggggtc
    14401 gagcatgacg ttaccgtggc gcagctcgtt gctggcctgg ccgttggttc ggcgcatcag
    14461 agcctccagc ctggcggcga cttctatcaa cgcaaaaggt ttacacagat aatcgtcagc
    14521 tcccagacgc agcccttcta cacgttccgc taacgcatca cgcgcggtca ggatcagtac
    14581 cggctcacgc tgacctttct ctcgccattc gcgcaaaata tcgcgaccat ccatgcctgg
    14641 taaggttaaa tccaggatca ccgcatcata aggtgcgcta taaagcgcct cttttccctg
    14701 acgaccttgt gtaaaccagt cgacactaaa acccatttta ctaaggccag ttttgatgcc
    14761 gtcgccaatc agcatgtcat cttctatcag taaaattcgc attttttcat ccctgcgata
    14821 accgtatttg ctgccagtaa accgtgttgt aacgcgctct gtacagcatt aaattttatt
    14881 tttttccctt aagaagttgt taaggactat cttgttaaat gctcggaaac agacattaaa
    14941 gggagtaata aacatgaaaa aattcgcagc agtaatcgca gtaatggccc tgtgcagtgc
    15001 accggtgatg gcagcagagc agggcggttt ttctggccca tcggcaacgc aaagtcaggc
    15061 cggaggattc caggggccga acggcagcgt aacgactgta gaaagcgcaa aatccctgcg
    15121 tgacgacacc tgggtaaccc tgcgcggcaa tatcgttgaa cgcatctctg acgatctcta
    15181 cgtgtttaaa gatgccagcg gtactatcaa tgttgatatc gaccacaaac gctggaacgg
    15241 tgtgacggtg acgccgaaag atacggttga gattcagggt gaagtcgata aagactggaa
    15301 ttctgttgaa attgacgtca aacagatccg caaagtaaat ccgtaattgt taccgctccc
    15361 gggacgcgtt cccgggaata atttcgcagg gaggcaaaat gacaaacctg acactggatg
    15421 taaacattat cgatttccca tcaatccctg tggcgatgtt gccgcaccgc tgtagccctg
    15481 agttgctcaa ctacagtgtg gcgaaattta tcatgtggcg taaagagacg gggctttctc
    15541 ctgttaacca aagccagact tttggcgtcg cctgggacga ccctgccacc accgcaccgg
    15601 aagcgtttcg ctttgatatc tgcggcagcg ttagcgaacc gattcccgat aatcgttatg
    15661 gtgtgagcaa tggtgaactt accggtggac gttatgccgt ggcccgccac gttggcgagc
    15721 tggacgatat ttcacacacg atatggggca ttattcgcca ctggctgcct gcaagcggcg
    15781 agaaaatgcg taaagcaccg attctgtttc actacaccaa tcttgccgaa ggggtgacgg
    15841 agcagcgact ggaaacggat gtttatgtgc cgttagcgtg atggggaatc tttaccggag
    15901 tgtgagccaa atcactcgcg ataagtttga gcgaacaatg ccacaggcgt attgttgttg
    15961 ggcttataat ttctccggta aggaatacca tgtatacgcg aaatttatta tccggtgcgc
    16021 cgtaagccgt ctcgggggta cccatgagca agccgcactt atggcaggag gcgcggggca
    16081 ctggtggaga cacagctaaa ctgggctgaa ccatatactt acagtcgact actttgatgc
    16141 tttaggcttc cctggactcg cataacctca acttctcgaa tcgcccggag cggaccccca
    16201 tgccgagtgg tgtggcatgg gcgcaacctt gtgctgccct ctatgccgat caagtctaca
    16261 gcaaagagtt gtatatcaag gcacattgat gcctttatcg ccgtcggagc acgtccggcg
    16321 gcgctattca cgctgatctc ctgtgactcg acgcggcaga taatgtagta tctccggcaa
    16381 tattgcccct ttgaaggctg gcgaatcagt tgaggaatca gaattaatga gcgatatggc
    16441 agagcgcctt gcgctacatg aatttacgga aaacgcctac ttaaactact ccatgtacgt
    16501 gatcatggac cgtgcgttgc cgtttattgg tgatggtctg aaacctgttc agcgccgcat
    16561 tgtgtatgcg atgtctgaac tgggcctgaa tgccagcgcc aaatttaaaa aatcggcccg
    16621 taccgtcggt gacgtactgg gtaaatacca tccgcacggc gatagcgcct gttatgaagc
    16681 gatggtcctg atggcgcagc cgttctctta ccgttatccg ctggttgatg gtcaggggaa
    16741 ctggggcgcg ccggacgatc cgaaatcgtt cgcggcaatg cgttacaccg aatcccggtt
    16801 gtcgaaatat tccgagctgc tattgagcga gctggggcag gggacggctg actgggtgcc
    16861 aaacttcgac ggcactttgc aggagccgaa aatgctgcct gctcgtctgc caaacatttt
    16921 gcttaacggc accaccggta ttgccgtcgg catggcgacc gatattccac cgcataacct
    16981 gcgtgaagta gctcaggcgg caatcgcatt aatcgaccag ccgaaaacca cgctcgatca
    17041 gctgctggat atcgtgcagg ggccggatta tccgactgaa gcggaaatta tcacttcgcg
    17101 cgccgagatc cgtaaaatct acgagaacgg acgtggttca gtgcgtatgc gcgcggtgtg
    17161 gaagaaagaa gatggcgcgg tggttatcag cgcattaccg catcaggttt caggtgcgcg
    17221 tgtactggag caaattgctg cgcaaatgcg caacaaaaag ctgccgatgg ttgacgatct
    17281 gcgcgatgaa tctgaccacg agaacccgac ccgtctggtg attgtgccgc gttccaaccg
    17341 cgtggatatg gatcaggtga tgaaccacct cttcgctacc accgatctgg aaaagagcta
    17401 tcgcattaac ctcaatatga tcggtctgga tggtcgtccg gcggtgaaaa acctgctgga
    17461 aatcctctcc gaatggctgg tgttccgtcg cgataccgtg cgccgccgac tgaactatcg
    17521 tctggagaaa gtcctcaagc gcctgcatat cctggaaggt ttgctggtgg cgtttctcaa
    17581 catcgacgaa gtgattgaga tcattcgtaa cgaagatgaa ccgaaaccgg cgctgatgtc
    17641 gcggtttggc cttacggaaa cccaggcgga agcgatcctc gaactgaaac tgcgtcatct
    17701 tgccaaactg gaagagatga agattcgcgg tgagcagagt gagctggaaa aagagcgcga
    17761 ccagttgcag ggcattttgg cttccgagcg taaaatgaat aacctgctga agaaagaact
    17821 gcaggcagac gcgcaagcct acggtgacga tcgtcgttcg ccgttgcagg aacgcgaaga
    17881 agcgaaagcg atgagcgagc acgacatgct gccgtctgaa cctgtcacca ttgtgctgtc
    17941 gcagatgggc tgggtacgca gcgctaaagg ccatgatatc gacgcgccgg gcctgaatta
    18001 taaagcgggt gatagcttca aagcggcggt gaaaggtaag agcaaccaat cggtagtgtt
    18061 tgttgattcc accggtcgta gctatgccat cgacccgatt acgctgccgt cggcgcgtgg
    18121 tcagggcgaa ccactcaccg gcaaattaac gttgccgcct ggggcgaccg ttgaccatat
    18181 gctgatggaa agcgacgatc agaaactgct gatggcttcc gatgcgggtt acggtttcgt
    18241 ctgcaccttt aacgatctgg tggcgcgtaa ccgtgcaggt aaggctttga tcaccttacc
    18301 ggaaaatgcc catgttatgc cgccggtggt gattgaagat gcttccgata tgctgctggc
    18361 aatcactcag gcaggccgta tgttgatgtt cccggtaagc gatctgccgc agctgtcgaa
    18421 gggcaaaggc aacaagatta tcaacattcc atcggcagaa gccgcgcgtg gagaagatgg
    18481 tctggcgcaa ttgtacgttc tgccgccgca aagcacgctg accattcatg ttgggaaacg
    18541 caaaattaaa ctgcgcccgg aagagttaca gaaagtcact ggtgaacgtg gacgccgcgg
    18601 tacgttgatg cgcggtttgc agcgtatcga tcgtgttgag atcgactctc ctcgccgtgc
    18661 cagcaacggt gatagcgaag agtaatatgc cggggagggc ctgtttcctc ccgcttgcaa
    18721 ggaacgccgg atgaaacgtt aagtcgtcat ccggcagcgt attcaacccc cggaaacgat
    18781 aaaaattccc ttgaaaccgt tgtttattca tgcgttgcga ttaacaatac gcttttccag
    18841 agagcggctt ttaacaatgc cctaacctga tttcaggtga cgtacaatgc cagtctctca
    18901 gaccttcaga gggtgttatg ctatatatct ttcgtcttat tattaccgtg atttacagca
    18961 tcttagtctg tgtattcggc tccatttact gccttttcag cccgcgtaac ccgaaacatg
    19021 tggccacctt tgggcatatg tttggccgtc ttgcgccgct gttcggcctg aaagttgagt
    19081 gccgcaaacc tgcagatgct gaaagctacg gcaatgctat ctatatcgct aaccaccaga
    19141 acaactatga catggtgaca gcatcgaaca ttgtgcaacc gccgacggtg acggtaggta
    19201 aaaagagctt gctgtggatc cccttcttcg ggcagttgta ctggttaacc ggcaacttat
    19261 tgatcgacag aaacaatcgc actaaagctc acggcaccat tgcggaagtc gtgaatcatt
    19321 tcaaaaaacg ccgtatttcc atctggatgt tcccggaagg aacccgcagc cgtggtcgcg
    19381 gcctgctacc gttcaagact ggagcatttc acgcggcaat tgcggcgggc gtcccgatta
    19441 ttcccgtgtg cgtctctaca acttcgaata agattaatct taatcgactg cacaacggtc
    19501 tggtgattgt cgaaatgctg ccgccaattg acgtcagtca gtatggcaaa gatcaggttc
    19561 gtgagctggc tgcccattgt cgttcgataa tggaacaaaa aatcgccgag ctcgataaag
    19621 aagtcgcaga acgcgaagcc gccggaaaag tttaagtcgg caatctgtat ttttgcgggg
    19681 aacactttcc tgcacggtat tactttagcc agttttacat ggagcaaata tgtcactcag
    19741 tcggcgtcag ttcattcagg catcggggat tgcactttgt gcaggcgctg ttcccctgaa
    19801 ggccagcgca gccgggcaac agcaaccgct acccgttccg ccgctacttg aatctcgccg
    19861 tgggcaaccg ctgtttatga ctgtacaacg tgcgcactgg tcatttacgc cagggacacg
    19921 cgcgtcggtc tggggaatca atggtcgtta cctggggccg actatccgcg tctggaaggg
    19981 cgacgatgtt aagcttattt acagcaaccg cctgacagaa aatgtctcaa tgacggtggc
    20041 cgggctacag gtacctggcc cgctgatggg cggtccggca cggatgatgt cgccaaacgc
    20101 tgactgggca cccgtactgc ctattcgcca gaacgcagct actctgtggt atcacgccaa
    20161 tactcccaac cgcacggctc agcaggtcta taacggcctt gccggaatgt ggctggtgga
    20221 agatgaagtc agcaagtcgc tgcctatccc caaccattat gatgtggatg attttccggt
    20281 cattatccag gataaacggc tggataactt tggtacgcca gaatacaacg aaccgggaag
    20341 cggcggcttt gttggtgata cgctgctggt taacggtgta caaagcccgt acgttgaagt
    20401 ctcgcgtggc tgggtgcgct tgcgactgct gaacgcgtcg aactctcgtc gctatcaact
    20461 acagatgagc gatggtcgcc cgttacatgt gatttctggc gatcagggat tcctgcctgc
    20521 tcctgtatcg gtgaagcaac tttcgctggc accgggcgag cgccgcgaga ttctggtgga
    20581 tatgagcaac ggcgatgaag tgtcgatcac ctgtggcgaa gcggcgagca ttgttgatcg
    20641 tattcgcggc ttctttgagc catccagtat tctggtttct accctggtgc taacgctgcg
    20701 cccaaccggc cttctgccgc tggtcacaga cagtcttccg atgcgcttgc tgccaactga
    20761 aatcatggcc ggttcgccga ttcgcagtcg cgatatcagt ctgggtgatg acccgggtat
    20821 taatggacag ctgtgggacg tcaaccgtat tgatgtcacc gcgcagcaag gaacgtggga
    20881 acgctggacg gtacgcgcgg acgagccgca agcgttccat attgaaggcg tgatgttcca
    20941 gatccgtaac gtgaatggtg cgatgccgtt cccggaagac agaggctgga aagataccgt
    21001 ttgggttgac ggacaagtgg agctgcttgt ttatttcggt cagccttcct gggcgcactt
    21061 cccgttctac ttcaacagtc agacgctgga aatggcggac cgtggctcga ttgggcaact
    21121 gttagtcaat ccggtaccgt aatcttcata ctcgcccgag gagggcgagt atggaatact
    21181 tttcgatttc tctttcgttc cggcttataa tccccggcct ttgattattt ttttatcatt
    21241 tctggaagca aaatgagctc tatctccctg atccaaccgg atcgcgacct gttctcctgg
    21301 ccgcagtact gggccgcctg ttttggaccg gcaccgtttt tgccgatgtc tcgtgaagag
    21361 atggatcaac ttggctggga tagctgcgac atcattttgg ttactggcga cgcgtatgtc
    21421 gatcacccaa gcttcgggat ggcgatttgc ggtcgtatgc tggaagcaca gggctttcgc
    21481 gtcgggatca tcgcccagcc ggactggagc agcaaagacg actttatgcg tctgggtaaa
    21541 ccgaatctgt ttttcggtgt tactgctggc aacatggatt cgatgatcaa ccgttatacc
    21601 gccgatcgcc gtttacgtca tgacgatgcc tacacgccgg ataacgtcgc gggtaagcgc
    21661 ccggatcgcg ccacactggt ttatacccag cgttgtaaag aggcgtggaa agatgtaccg
    21721 gtgatcctcg gcggtattga ggctagtctg cgccgtaccg cacattatga ttactggtcc
    21781 gataccgtgc gccgttccgt gctggtggat tcgaaagccg acatgctgat gtttggtaac
    21841 ggtgagcgtc cgctggtgga ggtggcgcac cgtctggcga tgggcgagcc gattagtgaa
    21901 atccgcgatg tgcgtaatac cgcgattatc gtgaaagagg cgctgcctgg ctggagcggc
    21961 gtggattcca cccgtcttga tacccctgga aaaatcgacc caatcccgca tccgtatggt
    22021 gaagatttgc cgtgcgcgga taacaaaccg gtggcaccga aaaagcagga agccaaagcc
    22081 gtaaccgtgc agccaccgcg cccgaaaccg tgggaaaaaa cctacgtgtt gctgccttct
    22141 ttcgagaaag tgaagggcga taaagtgctg tacgcccatg cttcgcgtat tctgcaccac
    22201 gaaaccaacc caggctgcgt ccgcgcattg atgcaaaaac acggtgaccg ctatgtgtgg
    22261 atcaacccgc ctgctatccc gctttctacc gaagagatgg acagcgtttt tgcgctgccg
    22321 tacaagcgcg tgccacatcc ggcctatggc aatgcccgta ttccggctta cgaaatgatc
    22381 cgtttttcgg tcaacattat gcgcggctgc tttggcggct gttctttctg ttctattacc
    22441 gagcacgaag ggcgcattat tcagagccgt tcggaagatt cgatcattaa tgagatcgaa
    22501 gcgatccgcg acaccgttcc aggttttacg ggcgtgattt ccgatctcgg tgggccaact
    22561 gccaacatgt atatgttgcg ctgcaaatcg ccacgcgctg aacaaacttg tcgccgtttg
    22621 tcgtgcgttt atccggatat ttgtccgcac atggacacta accacgaacc gacgatcaac
    22681 ctctatcgcc gtgcgcgtga tctgaaaggc attaaaaaaa tcctcatcgc ctcaggcgta
    22741 cgttatgaca tcgccgtgga agatccgcgc tatatcaaag agttggcgac ccatcacgtc
    22801 ggcggttatc tgaagattgc tccggaacat accgaagaag ggccgttgtc gaagatgatg
    22861 aagccgggca tgggcagcta tgaccgcttt aaagagctgt tcgataccta ctcgaaacag
    22921 gcaggtaaag agcagtatct gatcccgtat ttcatctctg cgcaccccgg tacgcgtgat
    22981 gaagatatgg tgaatttggc gctgtggctg aaaaagcatc gtttccgtct cgaccaggta
    23041 cagaacttct acccatcgcc gctggcgaac tcaaccacca tgtattacac cgggaaaaaa
    23101 ccgctggcga agattggtta taagagcgaa gacgtcttcg taccgaaggg cgacaaacag
    23161 cgtcgtttgc ataaagcgtt gttgcgttac cacgatccgg caaactggcc gttaatccgc
    23221 ccggcgctgg aagcgatggg caaaaagcat ctgattggca gccgtcgcga ttgcttagtg
    23281 cctgcgccaa ccattgaaga gatgcgtgaa gctcgtcgcc agaaccgcaa tacccgtccg
    23341 gcgttgacta aacatacgcc gatggcgacc cagcgtcaga cgcctgctac ggcaaaaaaa
    23401 gcgtcgtcta cgcaatctcg tccggtgaat gctggtgcga agaaacgccc taaagcggcg
    23461 gttggacgtt aaaaaaaatc cccggcaact gacatgctgc cggggatttt tttatcattc
    23521 tgcgtacata cgcaagcagc gcaggcaatc aaaatagtcc tgagcatcaa gacgcgtatt
    23581 ggctctgtta gcttcggatt tggcagcata gatttgtggg cgtagtgctt ttatatcctg
    23641 ctcaagccgg gcgattttgg catttaatgt ttgtacattg ctcgccagtt gattgacctt
    23701 ttgctcattg acgcagccac tgagcaagca ggttgccaga acaacagctc ccacggtgaa
    23761 aatcgttttc atatagtgtt cccttttatt tattgttccg gctaattgtt atttaattca
    23821 gctcattgct ttaatctggc ggcggttttt gcctcaatct gcttaagtag gctgccgctg
    23881 ttcgggcgga taaaatcagc gatttcccac ttgccttcgc gccgctgtaa aaccagttct
    23941 tgctcttcgt tgttgccatt ggtcagcgta aaacgaacat gaatgcgggc ggaatcggcg
    24001 gttttctcca ggactcgaat ttcatcaacg cgtgaaaata gcgactcttc gccaaaacag
    24061 atcggattgt cgtaaataaa ttgccgttct atttgcatat cttcatcggg attattggcc
    24121 gtcttgaagg tggttttata gaaggcatta accgcctggc ggtaagagtc ggaaaccacc
    24181 cacgtccacg gccagctata atcagcaata tgttcaaaga gttcggcaac aaaggcttcc
    24241 ggctgttctt tttgcaacga agctaaagcg gccagcgttt tttcattctc gctattaact
    24301 gcttgtaaga cgctgccatg attgctgaca atatcgtcaa tgacccaacg accattttcc
    24361 gccaccattt tcagtgtctg cgtggtcttt tctttatcat ctttaaagat acgaaagcgc
    24421 acaacggcat cggcatgatc ggcgtcagtt tgggttatcg caacgctttc tagcaccaga
    24481 tcgccaaaat cctgacaatc acaaaccgga tcataatcca gccagccaat attgcccggc
    24541 agcgtaagat tgtcgttcag ggtaagcgcc tgttgaatac gcgctgaagt tatcgcccgc
    24601 tctccggttt caccgaaata aggtgtactg gcatctgatt tatagttctg ataaatctgc
    24661 cggactgttt gttctacggt ctgtgaagga ggctgcgcgt gtacggtaaa acttgccagg
    24721 gctgctaaaa acagaagtat tattttcatc tgtttttcac ctctccgttg cgtttatccc
    24781 ggatatagat gagttgttct tttaagagct tccggct
//
LOCUS       DAZPBM010000047        24411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPBM010000047 DAZPBM010000000
VERSION     DAZPBM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.047"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            29..745
                     /locus_tag="ACHUZQ_001778"
     CDS             29..745
                     /locus_tag="ACHUZQ_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1025492.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            775..1275
                     /locus_tag="ACHUZQ_001779"
     CDS             775..1275
                     /locus_tag="ACHUZQ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR1025493.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1367..4051
                     /locus_tag="ACHUZQ_001780"
     CDS             1367..4051
                     /locus_tag="ACHUZQ_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1025494.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4048..5136
                     /locus_tag="ACHUZQ_001781"
     CDS             4048..5136
                     /locus_tag="ACHUZQ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1025495.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5219..6516)
                     /gene="exuT"
                     /locus_tag="ACHUZQ_001782"
                     /pseudo
     CDS             complement(5219..6516)
                     /gene="exuT"
                     /locus_tag="ACHUZQ_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            6999..8411
                     /gene="uxaC"
                     /locus_tag="ACHUZQ_001783"
     CDS             6999..8411
                     /gene="uxaC"
                     /locus_tag="ACHUZQ_001783"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1025496.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8426..9913
                     /gene="uxaA"
                     /locus_tag="ACHUZQ_001784"
     CDS             8426..9913
                     /gene="uxaA"
                     /locus_tag="ACHUZQ_001784"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1025497.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9996..10547
                     /locus_tag="ACHUZQ_001785"
     CDS             9996..10547
                     /locus_tag="ACHUZQ_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1025498.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10552..11796)
                     /gene="sstT"
                     /locus_tag="ACHUZQ_001786"
     CDS             complement(10552..11796)
                     /gene="sstT"
                     /locus_tag="ACHUZQ_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1025499.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12412..13377)
                     /gene="alx"
                     /locus_tag="ACHUZQ_001787"
     CDS             complement(12412..13377)
                     /gene="alx"
                     /locus_tag="ACHUZQ_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1025500.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13660..14646)
                     /locus_tag="ACHUZQ_001788"
     CDS             complement(13660..14646)
                     /locus_tag="ACHUZQ_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1025501.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14725..15408)
                     /locus_tag="ACHUZQ_001789"
     CDS             complement(14725..15408)
                     /locus_tag="ACHUZQ_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1025502.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15485..15988)
                     /locus_tag="ACHUZQ_001790"
     CDS             complement(15485..15988)
                     /locus_tag="ACHUZQ_001790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1025503.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16073..17209
                     /gene="rlmG"
                     /locus_tag="ACHUZQ_001791"
     CDS             16073..17209
                     /gene="rlmG"
                     /locus_tag="ACHUZQ_001791"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1025504.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17494..17808
                     /gene="higB"
                     /locus_tag="ACHUZQ_001792"
     CDS             17494..17808
                     /gene="higB"
                     /locus_tag="ACHUZQ_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1025505.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            17805..18221
                     /gene="higA"
                     /locus_tag="ACHUZQ_001793"
     CDS             17805..18221
                     /gene="higA"
                     /locus_tag="ACHUZQ_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1025506.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18266..20284)
                     /gene="fadH"
                     /locus_tag="ACHUZQ_001794"
     CDS             complement(18266..20284)
                     /gene="fadH"
                     /locus_tag="ACHUZQ_001794"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1025507.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20510..22861)
                     /gene="ygjK"
                     /locus_tag="ACHUZQ_001795"
     CDS             complement(20510..22861)
                     /gene="ygjK"
                     /locus_tag="ACHUZQ_001795"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1025508.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22878..23955)
                     /gene="ygjJ"
                     /locus_tag="ACHUZQ_001796"
                     /pseudo
     CDS             complement(22878..23955)
                     /gene="ygjJ"
                     /locus_tag="ACHUZQ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(24089..>24411)
                     /locus_tag="ACHUZQ_001797"
     CDS             complement(24089..>24411)
                     /locus_tag="ACHUZQ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025509.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
BASE COUNT         6169 a         6195 c         6256 g         5791 t
ORIGIN      
        1 ggaaacaata ttgtaagatc aaatttatat ggtggcgaaa attaatctct tatcacggct
       61 tacgccgctt ttattcgtgt tcgctccttt tttggcacag tcaaatatga ctgtttatcc
      121 tatggctgtt tccattaata gtcagggtga aggcaacgtt cgcgtaatat ctaaaagtaa
      181 tgaagttcag tacatcaagg cgacagtatt ccgtatcgat aatccatcga cgcctcagga
      241 aaatgaagtt gagattaaat ccggtgatgc aaatcatctg gttgttatgc cacctaaatt
      301 cgctttacca gccggtagta gcaaaactgt gcgctttgtt gcaatggaac cagagcaaaa
      361 agaaaaaaat tatcgcgtta aatttgaagc ggttcccagt attgatgacg ttgccacaga
      421 taaaaaagat ctctctatgc agttaacagt taacttaatt tgggggattg ttgttagtgt
      481 tccacctcag caacctattg ctaagttaga agtaaatgct gcccaaaaat tagttaatgc
      541 agggaatcaa cgcttaaaga ttttaacgat cgcttattgt aaaaataata gcaaagaaaa
      601 ttgtaagata cagaccgtaa ataaaaacat cttccttggt caggaaagaa atcttgaaag
      661 catatctggc tacgacaaaa tcgttgtcaa atataataac tggatcacca aagataatgg
      721 cgagtttgaa ctggcggtcc attaattatc attgtaataa acgaaggaat agtaatgaaa
      781 aaggtatttg caaaatctct tctggtcgca gcgatgtttt ctgttgcagg ctccgcattg
      841 gcagtacaaa aggatattac tgtaacggcc aacgttgatg ccgctctgga tatgacacag
      901 accgataaca ccgcgttgcc gaaagcagta gaaatgcaat atctgccggg gcagggtctg
      961 caatcttacc agttgatgac caaaatctgg tctaacgacg ttaccaaaga tgtaaaaatg
     1021 cagttagttt ccccggcgca actggttcaa agcctggatg ctagcaagat tgttccgctg
     1081 acagtaacct ggggcggtga agaaattaaa gctgatgcgg caaccacttt taccgcaacc
     1141 aaaatctttg cttccgatgc gttaactaac ggttctctgg ctaaaaacct gatgtttgct
     1201 caaaccacca aaggtgttct ggagacaggc atctaccgtg gtgtagtaag catttatctg
     1261 tctcaggata tctaatccag ataacagaat taacacgtaa caaagggagg ggtagccctc
     1321 ccttatttta actgttttag tatttgttat ggatttcagg attgccatgg ataaaaaatt
     1381 actggctctt ttgatcctgg cgagtctcag cccggcagag gcgacattaa ccgaaattcc
     1441 cgcagggttt gaggttattg ctcagggaca gcaggagtat atcgaggttt atttttcagg
     1501 gaaaagtctc ggtaaatatt atgcaatggt taatcttgat accgtaacat ttcttgatcc
     1561 agcaagtcta tataacaagc tggaactgga tgtagacgat cagaaaatcg cgcatatagt
     1621 gaaagaaaaa ttatcgcagc cgctagctcg ccacggtgaa ttggcttgcg gttatgtacg
     1681 tactgactca gggtgtggtt ttctgaatac cgatacgctg gaaataatct ataatgatga
     1741 agaaagttcg gcaacgttgt ttattaatcc gcaatggaat tcagctttcg atgcgaagtc
     1801 attatattta aatccagaca aaaatactgt taatgctttt atacatcagc aagacatcaa
     1861 tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg cgctgggaat
     1921 aacagaaaat agttatattg gtgcacactg gaatttcaac ggttatgatg cagatgatgt
     1981 cagtgacagt aatgctgacg tcagcgatct ctattatcgt tatgattttt tacgtcgtta
     2041 ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag gcgggaactt
     2101 tacctttaac tttctgccac tcggtgcaat tgatgggatg cgtatcgggt cgacactgag
     2161 ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc tttcgcgcaa
     2221 ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc tcaatagtgg
     2281 ttcgcaattt attgatacca gttcctttcc gccaggcagc tacagcgtgg cattaaaagt
     2341 ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa ccggcggtct
     2401 gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac aggtttctga
     2461 tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc aatatgagct
     2521 ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta ataactggac
     2581 ggcagattta ggcggggtgg ggaatcttgc aatcagcgcc agcgtgttcc gtaacgatga
     2641 cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc cgacgttggg
     2701 cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag agagctataa
     2761 cgccttaagc tgttatgaaa gtatttccgc gacggtttca cagaattttg tcggctggaa
     2821 tatgatgctg ggttataccc gcacacaaaa taacactgat gatagtttgc gttgggataa
     2881 acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta tctctgaaac
     2941 tgtacaactt agcgcttccc gcgcttttgt gatgcgtgac tggattttga gtacgtcagt
     3001 cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct tgtacttatc
     3061 gttttcgtta tctgacacgc caacgatgga tagcaataac aacagccatt caacaaatgt
     3121 gtctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc agttatcgca
     3181 taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg gaggcctgaa
     3241 caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg gaaatgtcta
     3301 cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg cctttacggg
     3361 cacttatagc tctacgctgg ctgtcagttg ctatggcgtt aatttgggag ccagtggtac
     3421 agacgatttg ctgggtgcgg tactggtgga tgtgaaaggc ttctctgaac aggatgaaga
     3481 gagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc agcttggtca
     3541 aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg aggttaacga
     3601 cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag ggaatcgtga
     3661 attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct ttaactacaa
     3721 ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg tggggctgaa
     3781 cagcgccatg ttactggtag ctgaagatgg aggatttaca cttgaaatta acggcagcga
     3841 aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga gtgttgtaaa
     3901 gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga cttattcaca
     3961 attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac gtggaaacgt
     4021 tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc gatattgggc
     4081 ttgtttggca cgctcattgg cgttcaggca gctcctgacg tgaccagtga aattacgtat
     4141 gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc ggcaggaaat
     4201 aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg tacagctaat
     4261 ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat gcgttggccg
     4321 gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc gccaggatgc
     4381 aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc atgtacagaa
     4441 gatggtcaac attattatat atacgataca aagtttctta cgctctacct tgagcagaca
     4501 gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt acattcgaac
     4561 agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc caaccatatt
     4621 gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga cttgcatcca
     4681 acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga catgtgcctg
     4741 tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga tgaagggcga
     4801 ccagctgcag ggcgcagaga cggtgacttc tctatctatc gtcaaggagg gaccaccacc
     4861 gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac cggtgggcaa
     4921 attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa acgtgtgcgt
     4981 ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc cttgactctg
     5041 gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg caaattgtca
     5101 gtaatcttta cgccttcctt gccaaccatc aattgacccc gcagaccaga aaaaactaaa
     5161 caacgaagcc gcctgataag gcggcttcgt cattacaact tacgttaaac gttaaacgtt
     5221 aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac ctcaatcgcc ggtttgttct
     5281 gcaatacggt ccagataacc agcgcaccca gcaggtcaaa tactgccaga actgcgaaca
     5341 gcgggctgaa gccgatggtg tcagccagtg caccgacaac cagcgcaaac agcgtacttg
     5401 ccagccatgc ggacatcccg gttaaaccgt tcgccgtcgc cacttcgtta cgaccgaaca
     5461 cgtcagaaga gagcgtaatc agcgcgccag acagtgcctg gtgggcaaaa ccaccgatac
     5521 acagcagcat aattgcgaca tacgggttgg tgaacagacc gatcataccc gggccaatca
     5581 tcagcacagc acccagcgtt acgaccatct tacgggaaac gatcaggttc acaccaaacc
     5641 aacgctggaa cagcggcggc aggtaaccac cgaggataca accgaggtca gcaaacagca
     5701 tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa gccgtaaact ttaaacatga
     5761 acagcgggat ccacgcgtta aaagtacccc aggccggttc tgccaggaaa cgcggcagtg
     5821 cgatacccca gaactgacgg ttacgcagga tctgaccaac ggacattttc ttcgccgtgc
     5881 tcacctggtg ctgggcttcc tgaccattaa taatatagtc gcgttcttca tcggtcagat
     5941 gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc catcgcccag ataaagctca
     6001 atgcaccgga gatgataaat gccatctgcc agctgtgcat cacgattgcc ataccaccag
     6061 cggcggcgca atcatcgcac caatcgaaga acctacgtta aagtagccta ctgcgatgga
     6121 acgctctttc gccgggaacc attcggagct ggctttcaga cccgccggga tcatcgctgc
     6181 ttccgcggca ccgaccgcac cacgagcaac agccaggcca ccccagctac ctgccagcgc
     6241 ggttacacca cagaacacgg cccacagcac agcaaacatt gcataaccga ttttcgtacc
     6301 cagcacatcc agtacataac ctgctaccgg ttgcatgacc gtataagcag cagaataggc
     6361 tgcgatgata taggaatact gttgggtgga gatgtttaac tcttccatca gagttggcgc
     6421 agctgccgcc acagtgttac gcgtcaggta accaagcacg gtgccgagcg tcaccagtgc
     6481 gatcatatac caacgtaacc ctttaatttt acgcatgtaa aacctcatcg ttttgttata
     6541 tccgcaggaa gcgcggccat catcccgcca gaggctgaag atgttagtta cgaccgggta
     6601 accgaaaaaa aaccggcacg ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt
     6661 ccgtaccggt taacccgatt catctaacaa atgaatgaat cggcaatttg cattccgtcg
     6721 acttatgatt tgcgacggca gaaagataac ttgtcataca actttaaaag gtgagagcca
     6781 tcacaaatgt gcgaatattt gtagggacat tacctgacga cagcgaggcc agtactggcg
     6841 cggcctgcag cgagatttat cactttgaga gtaatttttt taactacgtt tattgatcta
     6901 actcacgaaa atatcttcgg actctggaaa ttggtgtgat aactttgtca gcatcgcacc
     6961 ataagcaagc tagctcactc gttgagagga agacgaaaat gactccgttt atgactgaag
     7021 atttcctgtt agataccgaa tttgcccgcc gtctgtatca cgactacgca aaagaccagc
     7081 cgattttcga ttaccattgc catttgccgc cgcagcagat tgcggaagac tatcgtttta
     7141 aaaacctgta tgacatctgg ctgaaaggcg atcactacaa atggcgcgct atgcgtacca
     7201 acggtgtggc cgagcgtctg tgtaccggtg atgcatctga ccgtgaaaaa tttgacgcct
     7261 gggcggcgac tgttccgcac actatcggca acccgttata ccactggacg caccttgaac
     7321 tgcgtcgtcc gtttggtatc actggcaaat tgctttctcc gtcaactgcc gatgaaatct
     7381 ggaacgaatg taacgaactg ctggcgcagg ataacttctc cgcgcgcggc atcatgcagc
     7441 agatgaacgt gaaaatggtc ggcaccaccg atgacccgat cgattctctg gagcatcacg
     7501 cagagatcgc caaagacggc tctttcacca tcaaagtgct gccgagctgg cgtccggaca
     7561 aagccttcaa catcgaacag gcgaccttta acgactacat ggcgaagctg ggcgaagttt
     7621 ccgataccga cattcgccgc tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc
     7681 acttcgccgc tcacggctgt aaagtgtctg accatgcgct ggacgtggtg atgtttgctg
     7741 aagcgaacga agcggaactg gacagcattc tggcgcgccg tctggctggc gaaaccctga
     7801 gcgagcacga agtggcacag ttcaaaactg cggtactggt gttccttggt gccgaatatg
     7861 cacgtcgcgg ctgggtacag cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc
     7921 agttcaaact gctggggccg gatgtaggct ttgactccat caacgaccgt ccgatggcgg
     7981 aagagttgtc taagctgctg agcaagcaga acgaagaaaa cctgctgccg aaaaccattc
     8041 tctactgcct gaacccgcgc gataacgaag tgctgggcac catgatcggt aacttccagg
     8101 gcgaaggtat gccgggcaaa atgcagttcg gttccggctg gtggtttaac gatcagaaag
     8161 acggtatgga acgtcagatg acccaactgg cgcagctcgg tctgctgagt cgctttgtcg
     8221 gtatgctgac tgacagccgt agcttcctgt catacacccg tcacgaatac ttccgccgca
     8281 ttctgtgcca gatgatcggt cgctgggtgg aagcgggcga agcaccggcg gacatcaacc
     8341 tgctgggcga gatggtgaaa aatatttgct ttaacaatgc gcgtgactac ttcgccattg
     8401 aactgaacta aggtctgggg ttgatatgca atacatcaag atccatgcgc tggataacgt
     8461 cgcggtcgct ttagcagatt tggctgaagg cacagaagtc agtgtcgata accagactgt
     8521 tacgctgcgc caggatgttg ctcgtggaca taaatttgcg ttaacggata tcgcaaaagg
     8581 ggccaacgtc atcaaatatg gcctgccgat tggttatgca ttggcggata ttgcggcggg
     8641 ggaacacgtc cacgcccaca atacgcgcac gaatctgagc gatctggatc agtatcgcta
     8701 tcaacctgat tttcaggatc tgcctgcgca agcggcagat cgtgaagtgc agatctatcg
     8761 tcgcgctaac ggcgatgtcg gggtgcgtaa cgagctgtgg atcctgccaa ccgtgggctg
     8821 tgtcaacggc atcgcgcggc aaatccagaa ccgtttcctg aaagagacca acaacgccga
     8881 aggtaccgac ggcgtgttcc tcttcagcca cacctacggc tgctcacaac tgggcgacga
     8941 tcacatcaac acccgcacca tgctgcaaaa catggtgcgc cacccgaacg cgggcgcagt
     9001 gctggtgatt ggtctgggct gtgaaaacaa ccaggttgcc gcattccgtg aaacgttggg
     9061 cgatatcgat cctgaacgcg ttcatttcat gatctgccaa cagcaggatg atgagatcga
     9121 agccgggatc gagcatttgc atcagctgta taacgtgatg cgcaacgata aacgcgagcc
     9181 aggcaaactc agcgaactga agtttggtct ggagtgcggt ggttctgacg gtctttctgg
     9241 tattactgct aacccgatgc tggggcgttt ctctgactac gtgattgcta acggcggtac
     9301 taccgtactg accgaagtgc cggagatgtt tggcgcagag cagttgctga tggaccattg
     9361 ccgcgacgaa gcaacgtttg aaaaactggt caccatggtc aacgacttta aacagtactt
     9421 tattgcccat gatcagccga tctacgaaaa cccatcaccg gggaacaaag cgggcggtat
     9481 caccacgctg gaagacaaat cacttggctg tacccagaaa gcgggttcca gtgtcgtggt
     9541 tgacgtgctg cgttacggcg agcgtctgaa aacgcctggg ctgaacttgt taagtgcgcc
     9601 gggtaacgat gccgtagcga ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt
     9661 cagtactggt cgcggcacgc catacggtgg atttgtgccg acggtgaaaa tcgccaccaa
     9721 cagtgaactg gcggcgaaga aaaaacactg gatcgacttt gacgcgggtc agctgatcca
     9781 cggtaaagcg atgccgcagt tgctggaaga atttatcgac accatcgttg agtttgccaa
     9841 cggtaagcaa acttgcaacg agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg
     9901 cgtaacgcta taaagtcgaa atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc
     9961 gccgtttgct tttccatacc tgataaggat ttatcatgac cgcgtattgg ctggcccagg
    10021 gcgtgggtgt catcgccttt ctgattggta tcacaacatt tttcaatcgt gacgaacgtc
    10081 gcttcaaaaa gcagctttcg gtctatagcg ccgttattgg cgtacatttt tttcttctgg
    10141 gcacctatcc cgctggtgcc agtgccatcc ttaatgccat tcgtacattg attaccttac
    10201 gcacgcgcag cttatgggta atggcgattt ttattgtgct gactggcgga attggcctcg
    10261 cgaagttcca tcatcctgtc gaactattgc cggttatcgg tacgattgtc agtacctggg
    10321 cgctgttccg ctgtaaaggg ctcaccatgc gctgcgtgat gtggttttca acgtgttgct
    10381 gggtgattca caacttctgg gcggggtcga taggcggcac aatgattgag ggtagttttc
    10441 tgcttatgaa tggcttgaat atcattcgtt tctggcggat gcagaaaagg ggaattgatc
    10501 cgtttaaagt agagaaaacc cctcccgccg tagacgaaag gggttaaaca attaattacg
    10561 cagggcgcta tttgccagac gatcgtcttc cgcctggcaa gctgccgcag tgaacagcac
    10621 gtcagttgaa gagttcagcg cggtttcgca agagtcctgc aatacgccga tgataaagcc
    10681 gacggcaacc acctgcatgg cgatatcgtt cgaaataccg aacatattac aggccagcgg
    10741 gatcagcagc agagaacccc ccgccacgcc ggatgcgcca caggcacaca gagaagccac
    10801 cacgctcaat agcagcgccg tgggcagatc gaccgggata cccagcgtat taaccgcagc
    10861 cagcgtcaac acggtaatgg tgattgctgc gcccgccata ttgatggtgg cacccagcgg
    10921 aatagaaacg gaataggtat cgcgatccag attcagcttt tcacacagtg ccatattcac
    10981 cggaatgttc gccgcagagc tgcgggtgaa gaaggcatac acgccgcttt cgcgcaggca
    11041 cagcagcacc agcgggaacg ggttacgacg aattttccac cacaccagca atgggttaac
    11101 caccagcgcc accagtaaca tacagccaac cagcacgacc agcagttgcg cgtagcccca
    11161 cagtgtggag aaaccggtgg ttgccagggt agaagaaacc agaccaaaaa taccaatcgg
    11221 tgcgaagcga atgaccagtt tcaccataaa ggtgacggca ttcgacatat cgttgaccag
    11281 gtttttggtg gtctcgttac cgtgacgcag tgcgaaaccg aggccaattg cccacaccag
    11341 gatcccgatg tagttacctt tcagcagcgc gtcgatgggg ttggaaacca tgctcattac
    11401 cagcccgcgc atcacttcta caatgcctga cggtggcgaa atatcacccg cgctactgga
    11461 taagtgcagg gtagaaggga aggcgaagct gaaaacgact gcggccagag cagcagagaa
    11521 ggtacccagc agatagagga acaaaatagg gcggatattg gttttctgcc cgtgctggtg
    11581 gttagcaata gatgccatca ccagcatcaa caccaggatg ggggcaacgg ctttcagtgc
    11641 gccgacgaac aaagtaccta acagaccaac agcttccgcc gcgggttttg atatccatgc
    11701 cagaagaatc cccagaacaa ggccgaccag gatttgtttt accaggctgc catgagccag
    11761 acgccggaat agccccggtg aacgttgcgt agtcattttt cgatcctttc attgtgttat
    11821 cgcacatccc tggtgcgttc tgtcgacgca tctttcagga tgtaacaaag tgtttgcaag
    11881 gtcgagtata aggaaagtta cagcttcggg aagcgaaaaa tgctggattt taaagagaag
    11941 tcatattttg aaacttaatg gttacatttg tttgacataa acagagggaa ggactggcta
    12001 ctaaggctga ttagataaca tatgggcaaa actatttgga atatgagaac ggttgcgctc
    12061 ctttaatgtg ctatgccata taaagtcact ggcgcaggga gcgcgcaggg ggcggccaat
    12121 cgccgccgcc ccctgcaccc ccaggctctg gcgaacaaaa tcgccgctgc gcgattccct
    12181 cagcttatcc cttgcggcta ccgggtcggg cgcaagggaa cgtccctgta agatgcgccc
    12241 tcgccgcgca tccatgcgcg tcgccccggc ctccagggaa cgcttcggcg attttgacgc
    12301 caccaacacc cgtgcggtct ttttgtaaag agctacaagc cgattacata attaatagct
    12361 cacctttgtc tggaatgagt tatctctagg aatggcttaa tatacagcga atcattctcc
    12421 ccgctgctta tcatgccgat aattcaccca ggcgttgata ataaacgtca tcaccagaat
    12481 gccaaacacc acgcccagcg agacggcgat tggaatatgg tagaagtcga caatcagcat
    12541 cttgataccg ataaacacca gaatcaccgc caggccatat ttgagcatcg agaaacgctc
    12601 tgctacgccc gccagcagga aatacatcgc acgcaggccg aggatcgcaa acaggtttga
    12661 ggtcagcaca atgaacgggt cagtggtcac ggcgaagata gccggaatgc tatccacggc
    12721 gaaaatcacg tcgctcaatt ccaccagaat cagtaccagc atcagcggtg tggcgtacag
    12781 caatccattc ttacgcacaa agaaatgctc gttgtcgatg gtgtcggtca tgcgcaaatg
    12841 accgcgtagc cagcgcacca gcggcttgtc gccaatgcct gattcatctt catgggcgag
    12901 cgccatcttc acgccggtaa acagcaggaa ggcaccgaag atatacagga tccagtcgaa
    12961 ctgcgaaatt agccagctgc cagtgaagat catgatggta cgcagaacaa tcgccccgag
    13021 cacgccatac accagcacgc ggcgttgtaa tgccgccgga acagagaaat agctgaacaa
    13081 catcagccag acaaagacgt tatcgaccgc cagcgatttc tcaatcagat aacccgtgag
    13141 aaaggccagt gcctgtggat cggcaacggc gcgaccttca gtttgcacca gataccacca
    13201 gaaagcggcg ttaaacagta acgacagcgt cacccagacc agcgaccagg ccgcagcctg
    13261 tttcatggtc atggcatgtg ccccacgacg cccctgcaac aacaggtcga tagccagcat
    13321 aatggcgaca acaacagcga atccgcccca tagcaacggc gtgccgacag tattcataga
    13381 agttccttac acataaaaaa acggccaacg tcggaagacg tcagccgctg ctttttatgc
    13441 atagacctcg ccttccggca aggtctcact tacaacaaaa aaggaatacg tctgcgtatt
    13501 cctttcgggt tgcccggtga ccggatgtgg tttttcacac atcgtaatga cgatcgaccg
    13561 gcaatgaagt tactcccctt tgcaggtaac aaagtatgac agaccattca gccagtcaat
    13621 ggtctgttac atacctttta cactgtttta ctttggcaat tatagtttta cgctatctgc
    13681 cggaaaaatc accccagtct ggcggcggat ctcagtcagc agtttggcgg tgatgcgact
    13741 gaccgccagc cctggatgat ccaccagatg ctcatccacc agggtagcaa acagctctgc
    13801 ttcgtagagc atggtattaa tatgctgcgg ctgggtgaga tcctgcatct ggctgccacg
    13861 cggcacgaag cacactttct ggcattcaga cagtttttca atcaccagcg atcccgcttc
    13921 gccctgaatc tcgctttcca ggacagaatc actgaccttt gagtgctgca acgtaacgct
    13981 gaaatcaccg taatccatca ccaccacgcc gtgggcatct acgccgcttg ccaacaaact
    14041 ggcggttgcc tgcacgcttt tcggttcacc gaataatgcc accgccgacg ccaggcagta
    14101 aaagccgata tccataattg aaccgttaga gaatgccgga ttaaaggtgt tgggattctc
    14161 accatccagg taacgttgat accgcgagga atactggcaa tagttgaaaa agactttacg
    14221 caatttgccg actttcggca gcgcctggcg cagcaaatga aagttcggca ggctggcggt
    14281 tttaaatgcc tcaaacagca ccacctgatt ttcccgcgca caggcaatgg cggcatccac
    14341 ttccgccaga ttcgacgcca gtggtttctc gcaaatcaca tgaattttat ggctaaggaa
    14401 aagttgtgtc tgggaaaagt gcagggcatt cgggctggca atatacaccg cgtcaatggc
    14461 atcgctttcc gccatcgctt ccagcgaggt aaacagatgc tcgacagaaa aatcattggc
    14521 gaagtgctgg gcctgttcaa ggctgcggga atatacggcg gttaacttgt atttaccgct
    14581 ctcatgggcg gcctcgacga actggcgagt gatccagttc gtaccaatca cagcgaaacg
    14641 tatcataacg cgtggatact ccataaaggg attctgtcgt caatgtagca cgcctttttc
    14701 atcttcgacg tgcgcagata cgcattattc tttcgccgga cagccatgct cactaaacgg
    14761 cccaatcatg accgatggcc cgagatattt cggctggcga tggaggattt tggcgtcaat
    14821 caccgcgctg acacgagcga gtttctcccg cagttgagta taaaaaccgt gcttcatatt
    14881 aaggtctggc gcgttaaacc cgatagcgtc gatgccgtac tgttttgcca gccagatggc
    14941 gcgttggtta tgaaattctt gcgagataat ggtgatatgg ttttccccaa acaccttttt
    15001 ggcacgcacc actgaatcaa gcgttgagaa tccggcatag tcgcagaaga tcactttcgc
    15061 cggtacgcct ttagcgatca aagcctgctg catacctgag gcttcatcgt aatttttacg
    15121 tccgttatcg ccactgacta acagccattt cacttttccg acgtggtaca acgctgctgc
    15181 ggtatcaata cgtcgggtaa agtagcgatt acccggcctc gcccccagca ataaacccac
    15241 gttacgcgcc ggaactgcgt tgacatcgct ccaggtcagc tgcttgctgg cgttgaccat
    15301 tacgcgatcg gcgataaaaa tggtcgctcc tgccacaagt aacagtaagc aggtaatctt
    15361 tagagtccgg cgagaaaaac aaatacggag aagaaggcgg ggctgcatgg tcgttatcct
    15421 taacgaaagg ggggaggtcg attttaggcg ctaaagaaag aaaagataac gcctgacatc
    15481 attttcagat tttgtcctgc ccaagcgaca actgcgttag ccacagccgg gtgtcgaact
    15541 caagctggtg gtactgcggc tccatatggc agcacaactg gtaaaacgct ttgttatgct
    15601 ctttctcctt caggtgcgcc agttcgtgca ccacgatcat gcgtaaaaac ggttccggcg
    15661 cgttgcgaaa cacagtagcg acgcggatct ctgcttttgc tttcagcttg ccgccctgca
    15721 cacgagaaac agcggtatgt aatccgagcg cgtttttcag tacgtggatc ttattgtcat
    15781 acatcacttt agtgatcggc ggggcattgc gcagaaactg attttttaga tcctgggtat
    15841 attgccagag ggctttatcg gtggcgtagt cgtgtgttcc cggatagcgt tttgccagca
    15901 catcgcccag acgctgttcg ttaatcagcg ttcgcacctg ggaaagtagc tgctcgggat
    15961 aaccctgaag ataagtaaga ttgctcataa ccgcccacga aaaagagaaa aagggtatac
    16021 tcacgcaccc ttttcagggg caacgtcgaa attttatcat tcaggagggc cgatgagcca
    16081 cttagacaac ggtttccgtt cactgacact acaacgtttt ccggcgacgg atgacgttaa
    16141 cccgctacag gcgtgggaag cggcggatga atatttgctg caacagttgg acgacacaga
    16201 aatccgcggc ccggtgttga tcctgaatga tgcctttggt gcgttaagct gcgcgctggc
    16261 ggaacataag ccgtacagca ttggcgactc atacatcagt gaactggcga cgcgcgagaa
    16321 tttacgcctc aacgggattg atgagtcgag cgtgaagttt ctcgacagca ccgccgacta
    16381 cccgcaacag ccgggtgtgg tgctgatcaa agtgccgaaa acactggcat tgctggaaca
    16441 gcaactgcgt gcgctgcgca aagtggtcac gtcggataca cgtattattg ccggtgccaa
    16501 agcccgtgac attcacactt ccacgctgga gctgttcgag aaagtgctcg gcccgaccac
    16561 cactacgctg gcatggaaaa aagcgcgcct gattaattgc actttcaatg aaccgccgct
    16621 ggccgatgcg ccgcagaccg ttagctggaa gctggaaggt actgactgga ctatccacaa
    16681 ccatgcgaat gtcttctccc gcaccgggct ggatattggc gcacgcttct ttatgcaaca
    16741 tctgccagag aatctcgaag gtgagattgt cgatctcggt tgcggtaatg gcgttattgg
    16801 tctgacgctg cttgataaaa accctcaggc gaaagtggtg tttgtcgatg aatcgccgat
    16861 ggcggttgct tccagccgtt tgaacgttga aaccaacatg ccagaggcgt tagatcgcag
    16921 cgagtttatg atcaacaacg cgctctccgg cgtggagcct ttccgcttta atgctgtgct
    16981 ctgcaacccg ccgtttcacc aacaacatgc gctgaccgat aacgtcgcct gggagatgtt
    17041 ccaccatgcc cgccgctgcc tgaaaatcaa cggcgagctg tatattgttg ccaaccgtca
    17101 cctggattac ttccataaac tgaagaagat tttcggcaac tgcaccacca tcgccacgaa
    17161 taataaattt gtggtgctga aagcagtgaa gctggggcgt cgtcggtaag attgggagtt
    17221 acctgccgga tgcggcgtta acgccttatc cggcctacgt tcagcacgtt actcccaggc
    17281 cggataagac gcggcaagcg tcgcatccgg caatgtctgc ccagttctgg aatcgcgctt
    17341 ccataaaaca aaaaatccct ctcaccatcc tctacattct cttgtttagc gtttttctac
    17401 gtttattctt ccgtcacaca gataaattca tccgttgcca atctgtcaac ggatgttatc
    17461 atattgccaa tttgtcaacg gacgtgatga agaatgcacc tgataactca aaaagcattg
    17521 aaagatgctg cggaaaaata cccgcaacat aaaacggagt tggtggctct ggggaacacg
    17581 attgctaagg gatatttcaa aaaacctgag tcattaaaag cagtattccc atctctggat
    17641 aacttcaaat atctggataa gcattatgtt ttcaatgttg ggggcaatga attacgtgtt
    17701 gtagcaatgg tcttttttga atcgcaaaag tgctacatac gtgaagttat gacgcataaa
    17761 gaatacgatt tctttaccgc tgttcatcgt actaagggga aaaaatgatt gcgattgccg
    17821 acatcttgca agcaggagaa aagctaactg cggtggcacc ttttctggcg ggtattcaga
    17881 acgaggaaca atacacccag gcgctggaac tggtagatca tctgctgctc aacgatcctg
    17941 aaaacccctt gctggatctg gtgtgtgcca aaataaccgc gtgggaagaa tcagcgcccg
    18001 aatttgcgga atttaatgcc atggctcaag ccatgcctgg cggtatagcc gtgattcgta
    18061 cccttatgga tcaatatggt ttaacccttg ccgatctgcc ggaaattggc agtaaatcta
    18121 tggtgtcacg cgttttgagc gggaagagga aattaacgct ggaacacgct aaaaaattgg
    18181 caacgcgatt cggcatttct cccgccttgt ttattgatta acgcgacgtg ccggatgcgt
    18241 tgcatccggc gcttaaaata acgatttaaa tctccagcgc cagccgtgtt ccctgggcaa
    18301 ttgcccgtcg tgcgtccagc tccatagcca catcgcagcc gccaattaaa tgcacagttt
    18361 tcccgctatc aatcagcggt tgcgccagcg cgcggtttgg ctcttgccct gcgcagatca
    18421 ccacattgtc cactgctaat acctgcgttt cgccgttgat caccacatgc agcccgtcat
    18481 cgtcaatctt ctgataactt acgcctggga tcattttcac accccgcgag agcagggtgg
    18541 tgcgatggat ccagccggtg gttttgccta acccctgtcc cggtttgctg gctttgcgct
    18601 ggagcatcac aatttgccgt gggctacgcg ggatctgcat tccctgcggg cttaagccac
    18661 cagcctgttg taggctgctg tcgatccccc actcattaca gaacctggcg atattctggc
    18721 tggtggattc gcccggctga cttaaataca tcgccgtatc aaaaccaatc ccgccacaac
    18781 cgatgatggc aaccttgttg cctaccggcg ctttgtcgcg cagtacattg agataactca
    18841 ataccttcgg atgatcgatc ccgtcgatag gcggaacgcg cggcacgatc ccactggcga
    18901 ggatcgtttc atcgaacgcc tgtaactgat ccgccgtcac ggtgtgattg agtttcagcg
    18961 tcacgcccgt cacttcgatc atccggtggt aatagcgcag cgtttcgtaa aactcctctt
    19021 tgccggggat ctgtttggcg atattaaact gcccgccgat ctcgctgtgg gcatcaaaca
    19081 atgttacctg atgcccacgc gccgccgcgt taatggcaaa cgccagccca gcaggtcccg
    19141 caccgaccac cgccagattt tttttctgca cggcaggaag gattggcatt ttggtttcgt
    19201 ggcaggcgcg aggattcacc aggcacgagg tgactttgcc aacgaagatc tgatcgaggc
    19261 aggcctgatt gcagccaatg caggtgttga tctcatcggc tcgccccgat tgcgcttttg
    19321 acagcagttc cgcatcagca agaaacggtc gtgccatcga taccatatcg gcatcgccgc
    19381 gcgagagaat atcgtcggca acctgcggat cgttaatccg gttggtggtt accagcggca
    19441 gcgagacgtg gcctttcagt ttgcgcgtga cccagctaaa tgcgccgcgc ggcacgggcg
    19501 tggcaatggt cggaatacgt gcttcatgcc agccaatgcc ggtgttgata atggtcgcgc
    19561 ccgccgcttc aatggcctgc gccagctcta ccgtttcggc aaaagtcccg ccgtcttcga
    19621 ccaggtcgag catcgacagt cggtagataa taatgaagtc gttgccgacg cgttcgcgca
    19681 ccgcacgcac tacttctacg gcaaatcgca tccggttgcg gtaatcgccg ccccactggt
    19741 cactacgctg attggtgcgc agcgtcagaa attcgttgat caaataccct tcggacccca
    19801 ttacctctac accgtcgtat cctgcctccc gtgccagttg cgcgcagtgg gcgaaatcgt
    19861 cgatcagttg caggatctct tcatgggtga gttcgtgagg aacgaaacgg ttgatggggg
    19921 cctgcaatgc ggagggagcg accagatgcg gttggtagct gtagcgcccg gtatgcaaaa
    19981 tttgcagggc tattttgccg ccttcctgat gtaccgcttc ggtaatggtg cgatggtgtg
    20041 ggatctggct ggcgtcgttg agcattgcgc caccttccat gccaacgcct gttaaatctg
    20101 gtgcaatgcc gccgctgaca atcagcgcca cgccgtgacg ggcgcgttcg gcataaaacg
    20161 ctgccagccg ctcggcaccg tccgggtatt cctccagccc ggtgtgcatt gagcccatca
    20221 acacgcggtt ttttaacgtg gtaaaaccta aatccagcgg ggcgaacagc gacgggtagc
    20281 tcataaaatt gtccagtatg taaaataatt gttatgtggt cggatgagtt ctaatttagc
    20341 cgtcgatgga gaaaagggaa aaggggagtg cggggtttgt gatgggattc aaaaagaata
    20401 cgcccggtgg taacgccgga tgcaacgctt gccgcgtttt atccggcctg ggggattgca
    20461 catgtagggc ggataaggcg ttcacgccgc atccgccagc cgttgcaaat tactgcttac
    20521 ggaaaaaatc gttatacaac atatacaaat gagcggcact ccaggagaaa tttggtgcgc
    20581 cttgctgtgc gccagtcagc gggttgtaat tctcctgaat tgggccatcg gcggttaatc
    20641 ctttggcgtg ccggaagaac gtatccgcca gcttcagggc atcatcacga taaccgtaac
    20701 gctccatccc tttcagacca aaccagaact gatccaccca tacgcgccca cgccagtaga
    20761 tatcagcgcc aaaagccgga ttggttaacg ccgccgttcc cagcgggaca aaggtgttga
    20821 actctttagg atcgagcatc accttcacca ccgcgtcggc gttggcctgc gttgccgcac
    20881 cgttaaacag cggcgaccag ccttccggcc ctttaccgcg ctcaacaatc ggtttgcccg
    20941 cgcagccgtt cgccagtggt ttatcttcaa tacgtacgtc atagtagaac tgggtagtcg
    21001 ggtcgaacat acaggtgttg atgtagtccg cgagctgctg tgccaactgg cgatagcgtt
    21061 tggcctcttc tggcttaccg aggatggttg ccatctccgc cagataatgg ttatcgctgt
    21121 acatatagct ggcctgatcc accgactcct gcaatagcga gtagcccagc aacgttccgt
    21181 cctgactgcg gttttcggcg aatttcaccg tccagtcgct acgtttgccg ccattagcga
    21241 catatttatc cagctgttct ttgtcgataa acccaaagac ggcggcatca tcacgccccg
    21301 attcccacga tgcagcaacc tgtgccggaa tttccagact gtcgtacagg cctttctcca
    21361 ccacgcgggc gtagttgttc agcccagact gcgtctcttc tttatcgcct tttttcaccg
    21421 taaacagcat ctcgccgctc tcagtgttgt gggctttgtc gcgggtcgcg ccatattccg
    21481 gcacgccgtt gccgttgtga tcgcggttac gtaaccacca gtcgtgatag gccaccagtt
    21541 tcgggtacat ctctgccaac caggctttat cctgggtgac gttgtacact tccatcaccg
    21601 accaggcggc aaggctgggt ttggtattac gttcgttcca gttgccgcca tcgccaccac
    21661 gctcggggct aaggttccac gctatcaggt cggggacaaa gcccacatcc tgcggacgca
    21721 cgctatcgcc aggctggatc tgccaggaga agaccgcgcg gatattctct ttggcgatgt
    21781 ccggattgaa atgcgccatc gcaaacgcct gcttccaggt atcccacggc caggtctgat
    21841 tgccggagaa ccagcgcccg gtcaccgacg gtgtaacggt gttaaatttc accgcaccgc
    21901 ccggtgagag ccagttaccg ttgagcgttt cgatcgcttt caccgcgacg cgcgtctgtt
    21961 ccggcgtcgc atccggattg gttaaccctt tcttcaggta ttcttcccag cgttgctgcg
    22021 aggcggtgag ataaaacgcc ggacgcgcca gaatatcgcg gatctgcatt tgctctttgc
    22081 taacttcctg agcggtcagc agatgggaat aggtggtgta gagcgtggtc gaaccgttga
    22141 tatgcgcctt actggtaaag cgattaccgt tgatttcagt ctgcaccggc agggatttat
    22201 gcacctgata ttccgattcg ccggaggtca gcagatccca ggtggcgcgc actttgccaa
    22261 aggtgacttt caggccatca cgggtggcgc tgattttgcg ctgatagtca gggtattcgc
    22321 cagcaatggt tttatcggaa agcggtttcc cttctttcgc ttccagtttt tccagcagtt
    22381 cgccgtccca caccagatcc agcggtttat tgctggtgat tttggtttcc agtagtgacg
    22441 tgcgcggcgt ggcgaagcgc agagtcattt cgacttgcac atcttttgct gtcagttttt
    22501 gcaccagcgc accgggaata ctgtatgcct ccagcgtaaa gtcgactttc ttgccgtcct
    22561 gccagacggt caggcggtcg aaattgctgg ccataaagtt gatgtactct tccgtcagca
    22621 gcgcaacgcc cggaaagccg cccatggtgt tagggccgtc aggcaacaga tgaccatgcc
    22681 aggctccgag atcgaaaaac ggattaaaac gctggtgatc gtcgtaatcg taatccttca
    22741 tgtactgcgg cgcgccagta cggttaatca cgtttttata attgtcggcg ttagcggcat
    22801 gggcggaaaa acttatcagc agagcgcagg ttactggcgt taaaatagtt tttattttca
    22861 tgaaattaca gtccttatta ccagtagaaa tattgcatca ggaagatttg gtcgctggga
    22921 tcgtgagagg catttttcat gtagaagcgg ctatcaaaag ccgtggcgaa acgtccgccg
    22981 ccgtattcgt accagatacc aaactgcatg aaagaggtgg tttcatcgtc agcgtggtca
    23041 gggcgatgtt tggcgtaggt gtaagcggtg gagagataaa cgccctttaa cgcttcatac
    23101 atggcgctca ccatcatccc actttcactt cccggtacgt ccagcccgtc ggcgcgtccg
    23161 gtatggtgcc aggcgcgagc ggcaaagtta ggagttagca ggcccgtgag atagagctgt
    23221 cctgtgccgt cggtgatttc cagaccgttc atccaggtga tgccttcggc aaggtcatac
    23281 tggatgtaac cgttcatcat tgccggatag gtgtatttgt cgtcatagcg atcgtatttg
    23341 ccaaaatgta gccaggcttt gctttcatca tgatgtccgg caggagtcgc ggtgacgcta
    23401 taacgaaagt tgccgcttaa gttctgtacc ttcagcgcag tcataatgtc gcgggtattg
    23461 ggaatgacgt agcccagatc gggggtgaaa tcgccccacc attgcaggtc atccagcgag
    23521 gaatcctgac gcagactgag catgatttcg gtgccatcat tggtacgata accgccatag
    23581 aaacggttaa ttccgccctc gaaaccaccc cagctatggt ctggcaccca ggcattgccg
    23641 tcgtggtcgg cctgcacggt ccagccttcg ccgtaaatca ggccgaacca ggagccgtac
    23701 ttcatctcca ggccaccttc aatataggta ccgtcgctgt atttatgttt attctcaccc
    23761 tctaaatcca tatatccacc accccctaat ccctaattcg ccgtaaaaac gaaatgaagg
    23821 agaagatgtc gaggtaattg cgggtgtttt tgtgtcgtca tgttctgccg gacgtgcttc
    23881 ttctcctgcc gcagaaaagg cgtagcaaaa cggcagagca agtaatgctc tgcatggtgc
    23941 agtgattaat ttcattgtgt taatccatga gctaaagaaa aagtacagcc ttttatcgtt
    24001 gtaattataa aaatataaag gcagaaacta ttttccctct cctcatccag gaagagggaa
    24061 aatgcctgct cattttttat tattctgctt aatcaacatt gctggctggc gtcgcttcaa
    24121 tatggcgctc ttcagccgtt aatcccttta tatatttact gtatttccac cacgcaaagc
    24181 caaggaagat aacaataccg ccgacgttat aaaaaatgat ggtcagaata ttcgcgccag
    24241 tcgggaatgt cgaagcgaca aaccctacgg caaatatcgc aatcagcatt gaaacaacga
    24301 taataccggt gcggcgggag cccatacgga aatcgcgtgg caggtgatct aatttggcgc
    24361 gtaaattcag gtaagccagc atgatgaata acggcggaag catggacgct g
//
LOCUS       DAZPBM010000048        23848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPBM010000048 DAZPBM010000000
VERSION     DAZPBM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.048"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(74..1327)
                     /gene="amiC"
                     /locus_tag="ACHUZQ_001798"
     CDS             complement(74..1327)
                     /gene="amiC"
                     /locus_tag="ACHUZQ_001798"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1025510.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1559..2890
                     /gene="argA"
                     /locus_tag="ACHUZQ_001799"
     CDS             1559..2890
                     /gene="argA"
                     /locus_tag="ACHUZQ_001799"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1025511.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2952..4778)
                     /gene="recD"
                     /locus_tag="ACHUZQ_001800"
     CDS             complement(2952..4778)
                     /gene="recD"
                     /locus_tag="ACHUZQ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1025512.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4778..8320)
                     /gene="recB"
                     /locus_tag="ACHUZQ_001801"
     CDS             complement(4778..8320)
                     /gene="recB"
                     /locus_tag="ACHUZQ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1025513.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="ACHUZQ_001802"
     CDS             complement(8313..11201)
                     /gene="ptrA"
                     /locus_tag="ACHUZQ_001802"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1025514.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11377..14745)
                     /gene="recC"
                     /locus_tag="ACHUZQ_001803"
     CDS             complement(11377..14745)
                     /gene="recC"
                     /locus_tag="ACHUZQ_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1025515.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14758..15081)
                     /locus_tag="ACHUZQ_001804"
     CDS             complement(14758..15081)
                     /locus_tag="ACHUZQ_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1025516.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15066..15473)
                     /locus_tag="ACHUZQ_001805"
     CDS             complement(15066..15473)
                     /locus_tag="ACHUZQ_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1025517.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15470..16033)
                     /locus_tag="ACHUZQ_001806"
     CDS             complement(15470..16033)
                     /locus_tag="ACHUZQ_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1025518.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16024..16494)
                     /locus_tag="ACHUZQ_001807"
                     /pseudo
     CDS             complement(16024..16494)
                     /locus_tag="ACHUZQ_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16679..17473)
                     /gene="thyA"
                     /locus_tag="ACHUZQ_001808"
     CDS             complement(16679..17473)
                     /gene="thyA"
                     /locus_tag="ACHUZQ_001808"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1025519.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17480..18355)
                     /gene="lgt"
                     /locus_tag="ACHUZQ_001809"
     CDS             complement(17480..18355)
                     /gene="lgt"
                     /locus_tag="ACHUZQ_001809"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1025520.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18506..20752)
                     /gene="ptsP"
                     /locus_tag="ACHUZQ_001810"
     CDS             complement(18506..20752)
                     /gene="ptsP"
                     /locus_tag="ACHUZQ_001810"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1025521.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20765..21295)
                     /gene="rppH"
                     /locus_tag="ACHUZQ_001811"
     CDS             complement(20765..21295)
                     /gene="rppH"
                     /locus_tag="ACHUZQ_001811"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1025522.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(21648..21794)
                     /gene="ygdT"
                     /locus_tag="ACHUZQ_001812"
     CDS             complement(21648..21794)
                     /gene="ygdT"
                     /locus_tag="ACHUZQ_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1025523.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21980..22669
                     /gene="mutH"
                     /locus_tag="ACHUZQ_001813"
     CDS             21980..22669
                     /gene="mutH"
                     /locus_tag="ACHUZQ_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1025524.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22738..23451
                     /locus_tag="ACHUZQ_001814"
     CDS             22738..23451
                     /locus_tag="ACHUZQ_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1025525.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23589..23807
                     /gene="ygdR"
                     /locus_tag="ACHUZQ_001815"
     CDS             23589..23807
                     /gene="ygdR"
                     /locus_tag="ACHUZQ_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1025526.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5702 a         6525 c         5958 g         5663 t
ORIGIN      
        1 ttattgaaca ccctaacggg tgtttttttg tttctactct cccataaaaa aagcgccatt
       61 cagcgccttt ttatcatccc cttctcgcca gcgttgcccc atcggcaaaa tacgctttaa
      121 tccccgcaag aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct
      181 cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct
      241 ttagtacggc aaacccggcc tgttcaactt gatttttatg cagcttgttg attttaccga
      301 gcttattcag cactgcttta ccaaacttaa ggctgtcagc aatggtcagc gactgcacca
      361 tatcgaacat ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt
      421 ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgca cctttggttg
      481 agagcgcaaa caccgaggaa ccgctcggct ggcgactggt aaaggcgtcg gcatggatag
      541 agacaaacag gtcagcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga
      601 agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctctttctcg atcagagagc
      661 gcaaacggcg agctatttgc aataccacat ctttttcgcg cgttttgtat ttccccaccg
      721 caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc
      781 ctgctttacc tggttgtgga ccactttgcg ctggcggcac ctgcttttcg aggtcgcctt
      841 tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat
      901 agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg
      961 gttttacgtt ttgctttaat tcaaaaacca tccgtacggt ttgcgggtca aattgcccga
     1021 cgcgcgccga cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta
     1081 ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttcagggtta ctcaacgcga
     1141 actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag
     1201 gccagacgcg caccgccacg acctggctaa ccgcagccag gctgacctga cttacgctca
     1261 atagccacat agcgcccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc
     1321 ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt
     1381 gaccggaaac tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt
     1441 ttatattcac ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc
     1501 tgcgaaaaaa cagaataaaa atacaataat ttcgaataat catgcaaagg ggtgtgccgt
     1561 ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa
     1621 tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa
     1681 tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt
     1741 ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct
     1801 gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc
     1861 gggaacattg caactggata ttactgctcg cctgtcgatg agtctcaata acacgccgct
     1921 gcagggcgcg catatcaacg tcgtcagtgg caattttatt attgcccagc cgctgggcgt
     1981 cgatgacggc gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgat
     2041 ccgtcgtcaa ctggacagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac
     2101 tggcgagagc tttaatctga cctcggaaga gattgccact caactggcca tcaaactgaa
     2161 agctgagaag atgattggtt tttgctcttc acagggcgtc actaatgacg acggtgatat
     2221 tgtctcagaa cttttcccta acgaagcgca agcgcgggta gaagcccagg aagagaaagg
     2281 cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg
     2341 cgtgcgtcgc tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt
     2401 ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc
     2461 aacaatcaac gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat
     2521 tctggtgcgc cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca
     2581 gctcgataac acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg
     2641 ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct
     2701 gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac
     2761 cacgcgcagt attcactggt tccaggaacg tggatttacc ccggtggata ttgatttact
     2821 gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga
     2881 tttagggtaa gcgatgaaaa tcgccggatg cggcaggcgt aacactcgtg cgtcgcatcc
     2941 ggcaattacg tttatccccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta
     3001 cgagtggcga ttgccgcact taatatacgc tcatcggcat acagcgacag acggcgacgc
     3061 gcgcgggtca ccgcggtgta taccagctct cgcgttacta tcggcgtgcg ttggctcggt
     3121 aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac
     3181 gtagtttcgt gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc
     3241 gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac
     3301 aacccaagcg cactgtcatt acgggcaatc atcaccggtc gaccttcgta ccaacgagag
     3361 tgcgaatgac gatgaatttt gcgcttctgt tgcataaact gctcaattcg ctcattcagt
     3421 ccagccacgc caaatggccc ttcccgcagg gcgcacaaaa gttggtactc attgaacgcc
     3481 tgaatgatta aatccggctc ggcacgcgct tgcagcagat ccagataacg tccataaccc
     3541 gcaagagctt cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag ccgtttttca
     3601 atatcagtaa aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc
     3661 gccgcagcta actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg
     3721 cagagactgt cgcgcaaaga tgccgcttct gtgccggttc ctgccggaac gtgagttccc
     3781 gtcaggcggc ttagctgccc ggcacgctcg gcggtaaagc cggcattggc ataagcgcag
     3841 atatcgccca gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag
     3901 atcactcgcg catgatcggg caaggcgtcg atcagtctcg acatcatagg cagatcgatc
     3961 attgacgctt catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt
     4021 aaacgctggc tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga
     4081 atgcgtttct tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg
     4141 gttaagcgcg cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg
     4201 tcggccattt gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt accggtgcct
     4261 gggccgccgg aaatcaccga gatccgccgc gtcagcgcca ctgctgccgc cactttttgc
     4321 cagttaattt catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca
     4381 tcaacctcaa tggcatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac
     4441 cacatgcgat tcaaataaag acgatcgcca cagaggatca tcggcgtggg ttcatctccc
     4501 ctgctgaccg cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca
     4561 ctgacacagg tcgccaacag cggatgcgac gcctcgttat tttccagtcg tgaaagcggc
     4621 aaacagacgt gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca
     4681 ggatgttcat ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg
     4741 tgctccacag cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac
     4801 cggcaaacat ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc
     4861 cttgttgcgg atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt
     4921 gacgatcata gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat
     4981 aaagctgata ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg
     5041 tgtaagccga actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc
     5101 cttcgtggcg gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact
     5161 ccagcggcgg gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc
     5221 tggcgatgag cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt
     5281 cggaaagctg actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga
     5341 tccactcggt caacaccggt tcccactgcg attcaaagcc gccgagttcc agtttttcct
     5401 gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga
     5461 atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga
     5521 cgctgaccac gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt
     5581 gaccacgctg ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt
     5641 gtagtgtcct cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat
     5701 caccagtttg tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc
     5761 gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt
     5821 ggacgtcggt gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac
     5881 tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac
     5941 gttcgacctc cgcgaggtcg acgctttctg gcgcagcatt aagatccaaa actgcctcaa
     6001 acgagtggcg atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca
     6061 accagaccag cggatattcc agccctttcg atttatggat cgtgacaatc tgcaccagat
     6121 gtttatcact ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat
     6181 gttgcgataa ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgaa
     6241 gcagttcgct gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca
     6301 agttttctgc aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt
     6361 tgcgccagat ttgccgataa ccatcgaact cttcgactac cgcatcccac gcatgttcgt
     6421 cattattcag cgtttcaata tccagcgcat tcagccccat cattgacgtt gccagcgcac
     6481 tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg caacagccaa agcatttcct
     6541 gcgcttccag agtttcaaaa acactatcgc ggttcgaaag gtaaacggaa gggatttcca
     6601 gcaacgttaa ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacgctga
     6661 tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct
     6721 gtccggcttg tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat
     6781 agtcgccaac gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt
     6841 cacctttaaa gacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg
     6901 gtatttcgcg aaacatgaac gcgtcatcag tctggctgaa aagcttgttc acactgttca
     6961 ccattccagg tgcggagcgc cagttggtgt ctaaagtgta gtgggcgtga acttcactac
     7021 gcgccttcat ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt
     7081 cgccaatcag caacaatgcg gtttctggct gatggtgcca gatacggcga aaaattcggt
     7141 actgctgggg gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga
     7201 tcgccgctgc caacacctcg ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca
     7261 tgtcatcaaa acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct
     7321 cagccaatgc gcgggtgatc accagatcac ggatcgacaa tggttctgca agcagttgat
     7381 cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc ggctttcgtg cgatcttcta
     7441 agaaacgctg ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt
     7501 ctgcccaggc gctgatcttg tcgatccatt tagcctgatt gctacggtta aacttgcgtc
     7561 gatcaatacc agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct
     7621 gttttaccgt atcaatacgc gccacaattt gcgcgtgacg ggaagctagc gtttcatcat
     7681 cgggcggcgg tgctttgata accggcgctt cgccttgcag ataacgatta atatcgcgca
     7741 gcaacgcctg cggccctttc caggtttcaa agacgacctg ggctatttca cgcggcagcg
     7801 ggtagcagtg gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt
     7861 caatcagctg ctgctcaaac agcatgccgg attcaaaggc attcaggttg agcatgcgct
     7921 ggcaaaagcc gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca
     7981 accactgcgc ggcttgcgct ttatcgtcga tctcttccag caggtgttcg tacaatggat
     8041 tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac
     8101 cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacggtca
     8161 gcgggcgggg aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa taaagcgccg
     8221 caatcgtaaa ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg
     8281 gcaagcgcaa aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc
     8341 attgtttgct gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat
     8401 tcggctttcc cgttttggct gccggaaatc tgcgacagga tagccatgcc ttgcggctcg
     8461 actaccgcct gatggaagaa atcggccagt ttttgcggcg tcagcagttt aatctgggcc
     8521 acgattttat cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc
     8581 gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg
     8641 atttgcgcaa actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac
     8701 gccttgtaac gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc
     8761 atgccccact gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct
     8821 tcggtacgca actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag
     8881 gctgagctgg tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg
     8941 gtgctgttac cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg
     9001 tttcgacacc actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt
     9061 gttgcctggg cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt
     9121 aaggcgtcgc gataagccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt
     9181 tcatctcgcg agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca
     9241 aacgctttac ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca
     9301 agctgatctt ccgtagcggt atagctaaag tacccctcga gcaatgcctg gaacagctgc
     9361 ggcagacgct gggggtaacc attagcatta accataaggc cgttgttagc gttggtggaa
     9421 aaacttatgc caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga
     9481 taatcattga gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc
     9541 aaaatcaggc tgacatcagc tttgggttcg ctggaaaaat aacggcttgg cgcatacacc
     9601 acgcgcagat tcgactcatc aacaatcagc tctggatggt catatttctt ctctgactta
     9661 atcagcgaga aatcgtcagg aatatagggg ttaagctccg gtaaggagag cgcaatattg
     9721 gcagcttttt gctgccagtc agcgaaagtt tgttcgctga ttttatcgac ctgatacggc
     9781 gcatcgacaa aataagccgt tttattgtgc ggctctttcg ggctgatata ccagatacgc
     9841 gcattctgcg gcgtcatcat cgccagacgt tcttttactg ctttagcatc gtaccgatcg
     9901 gcaatattga ctgcatccag cgtatgctca accggaacgc gaatcatggt atctgccagc
     9961 cattcgacgt aatccatatc acgggtgatc gacggataac ggaagtcgat atccagcaca
    10021 ttcgccagtt catcgaagta ttgtttatca atgccttttt cacgtaacag attgagatag
    10081 ctaaaaattg ccgccacaac ctgatcgcga ttagccaggc ctttatcggt taaagacgca
    10141 gagatcgcta atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc
    10201 tcaactaatc cctgcttttg cagccagtcg gaaagtgtac ctgggctgcg attaccaatc
    10261 agataggtaa tcaattcatc cgttttacta cggaacttcg ctgagttgtt atcgatgcga
    10321 aactcaacgc gcagaacttt acgcggcatt gccgggacgt aatgaatgat aatgcccttt
    10381 tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg
    10441 cgaccaaagg tgtccgccgc catttttgcc aactccggca gcggtttatt gctgtaaata
    10501 accgccttca tcagattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc
    10561 accggattac caggtttatc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg
    10621 gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta
    10681 cgcgccatgg ttaattcagc gttcaccgca ttacgctcac gttcggcata tttcttgtcg
    10741 agcaaaggtt cagcaatagc atcggccagg cggtctaccg caccaggcaa ggcgtcgttc
    10801 tcaacttcca gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg
    10861 tgcattttga gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga
    10921 ctcatatgtt caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca
    10981 acgggcacca ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc
    11041 accataccgt tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg
    11101 atggtttcct gaatcggctg ccatcccgtt tctgcctgac ttaagggtgc ccaaagggca
    11161 actaacaaca ataatgcttt gaaccaggtg ctgcggggca ttctcggacc tcataatcaa
    11221 cttaattttc tgtccagatt caacacgtta acgcaaatca tctgccagaa tttaatcttg
    11281 tgctgcacga gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa
    11341 agtaagcgga tagattgcgc aatttttata cagcactcat gactgattaa agcgaaacag
    11401 cggtaacagg aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg
    11461 ccgccagagc ctttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc
    11521 gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc
    11581 atcgttttgc gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag
    11641 caatggcgcg gacatccctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc
    11701 ctgttcggct gcaagcggcg gaaaacgcca ctcgccgtct ttgcgtagaa aaaggcgact
    11761 ttcaccatta ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg
    11821 cgccacactt aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa
    11881 ccagccagtt atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg
    11941 gcgacaggca atgactctgt cggcaagctg ctgcatctcc tggcactgag tttcccagaa
    12001 aatttcacca aaagcgccat acggtaaatc ccctgccgcc cggaagcgac ggaacaagcg
    12061 ttcggcatca tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct
    12121 aagcccttcc agaataaatg gctcggtgtc ggggatttcg ctgtcttcag tacggaagtt
    12181 cacctgcaaa cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt
    12241 ttccagcggc acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc
    12301 tttaccagcc tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg
    12361 ctgatagttt tgtggatcaa acggcattcg ggtatggagg caagtaagat gcgcttttac
    12421 ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc
    12481 gatgtagtcg atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat
    12541 ggaacgcccg atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa
    12601 cagatagcgg tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag
    12661 gtcaaagccc aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca
    12721 aaccactttg aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc
    12781 gagaaaacgc tggctgatac gttcttgatc cagacgctgt gccagttcat cacgcaatag
    12841 tgacagcggc accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca
    12901 ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgct
    12961 gagcatatcg cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg
    13021 acgccagatg tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa
    13081 gccgctcgat tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc
    13141 gtagcccagc aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag
    13201 ttccagctcg cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca
    13261 ctggcgtaaa taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac
    13321 atccagtaat gccagcacgt cttccgacac aaagcggctg tctggcaacg ataacaggct
    13381 gataaacgct tccagtaccg gatgcgactg ccgcgcccga cggtcggaaa tggcgtaagg
    13441 caggtaacga tccgcaggtg cgctaccaaa cacagcccga ataaacggac tgtagctgtc
    13501 gatatcagcc accatcacga tgatgtcgcg cggagtaagt gtcgggtctt cttccagcat
    13561 cgccagcagg cgatcgtgca aaacttcaac ttcacgctgc gggctatggc aaacgtggaa
    13621 ggtgatactg ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc
    13681 gatgttcaca ccagcaacgg cacggttttc cagttccaga atgtcagact gaatgttatg
    13741 cagcaggtta tctggcgtga catcgacaaa ggcgtccagc tcctggctgc tctccaggtc
    13801 agaaaggaga taaatgtagt cgcgcccaag cttaccccat gaagccagca gcgggttgcc
    13861 gacatcctgt tcaccatcgc tgttaaagag ctgcccggca ttttcgctat cacgaaatag
    13921 cggcaattcg cgatcttcaa aactgtgtcg acgctgacgg gtcagcagtt tcgccagata
    13981 agcaggatct ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat
    14041 ttcaatatgt ttacccagcg cctgtagcgc ctggagataa acaggcggta acgcggaaat
    14101 accgcatata aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt
    14161 ttcgataaag cgctgataga gattggcgcg gtgccagcgc ggttgcccga gttgatgggt
    14221 atattccacc agcgccttcc acaacggggc ttgccaggcc tgtgcttctc ccagcccttc
    14281 aaccaaatgt cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc
    14341 aaacaggtcc gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagt
    14401 cagataatgc cgcaacaggg taaagtcttc gcgctccagc aattgcggca gcagagtcat
    14461 cagtttccag ctcatgctct gtttgttaaa ggcgctctct ttggggattt ccggtaacac
    14521 ccggacgaac atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat
    14581 actaaacttt tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag
    14641 aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa
    14701 cgcttccagc acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta
    14761 ctgacgattc gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag
    14821 cgtaacgctg atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt
    14881 ggcaggtggc gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagctgttg
    14941 gtactggttt ctgctggcga gactgttcat taatgttcgc tggatacccg ataacgcagt
    15001 gacaatcatc accatcagca ccatcgcaac cattacctcc ggcaggctaa agccttgttg
    15061 attcttcagg gaagctgaca taacgccctc tctttcagcg gacaaaaatc gctccagccg
    15121 cgtggcgaaa aaacaatgtt tccatcaatg acttcgcctg ttcgccacaa cgaaacgcct
    15181 tcataaccgg caaccaataa ggcttcatta tctgccagta aacgcaaaca aacccgggca
    15241 tcggttccag cgtactgcga gcactgaact gcgggctgcg tctgccagga gtgcattttc
    15301 ccccacgcca gcgccgactg aacgatggcc tggcggcgca atgactgact ttctatgctc
    15361 acgcgagagg caaaactgcg atcctgctga ctcattcctt gcaatagcaa gctacccaaa
    15421 accagcagca tcaggaccag agccagtgac gaaacacctt tttcgcggtt cacaggttga
    15481 atcctgtcac gctatagctg gcatccacca cggtttgcgg ttcagactta ctggcagcac
    15541 gcatattaac cgtcaacacc ggcgagaagc cgctgacatc ctgacgtacg acctgaaaag
    15601 tgtcgataat gatggcatcc ggattagtga ctttatccca gcccttacct tcacaggatg
    15661 tcgcaccgcg tagcgtttcc agcacatgct ccttcagacg aaatccaatc tggtcagact
    15721 cttttaccgg ttcgcgatcc cagataccgt tactgttcgc atcccactgc acaatgatac
    15781 agtcaccctg tccgacaatt tccagccctt cgccggtaca gctgccatga caataacccg
    15841 ccctctggag atgcttcgcg acggtaaata cccgcagcca gatttcatct tccagcgcca
    15901 gcttacgggt gctcgttaaa ctttcacgct gtaacgcagg cagaaagcgt gcagccccca
    15961 gcaacaatac gctactgatc gccatagcaa tcaacacttc cagcagagaa aaaccttgct
    16021 cttttacagg catccttctg tttctccttg ctgacaaagc cggagtcttc cccacggcga
    16081 aaccaccagc caccactcgc ccgttgagtt tttgaagcga atatgcccgg cccatgcggt
    16141 attgcgcagg ccaaagaaag caagcgaagg tgtcaggtcg ctcatttaga cttcgggcca
    16201 gcgtggcaca aagaccaatg gtgaactgcc atgacaggta ttggccccag caacggaact
    16261 cacaaggcac cataacgtcc cctccctgat aacgctgata ctgtggtcgc ggttatgcca
    16321 gttggcatct tcacgtaaat agagcaaata gtcccgcgcc tggctggcgg tttgccatag
    16381 ccgttgcgac tgctgccagt attgccagcc atagagtcca cttgcgctta gcatgaccaa
    16441 aatcagcatc gcgaccagcg tttcaatcag cgtataacca cgttgtgttt tcatgccggc
    16501 agtatggagc gaggagaaaa aaagacgagg gccagtttct atttcttcgg cgcatcttcc
    16561 ggactattta cgccgttgca ggacgttgca aaatttcggg aaggcgtctc gaagaattta
    16621 acggagggta aaaaaaaccg acgcacactg gcgtcggctc tggcaggatg tttcgtaatt
    16681 agatagccac cggcgcttta atgcccggat gtggatcgta gccttcaatc tcaaagtctt
    16741 cgaaacggta gtcgaagatg gattcgggtt tacgtttgat aatcaacttc ggcagcggac
    16801 gcggttcgcg gcttaattgc agatgagttt gatccatatg attgctgtac aggtgcgtat
    16861 cgccaccggt ccagacaaaa tcacccactt ccagatcgca ctgctgcgcc atcatatgca
    16921 ccagtaacgc gtagctggca atgttgaacg gcaggccgag gaagacgtca caggagcgct
    16981 gataaagctg gcaagagagt ttgccgtctg ccacatagaa ctggaagaat gcatggcacg
    17041 gtgccagcgc cattttatcc agttcgccta cgttccacgc tgaaacaata atgcggcggg
    17101 aatccgggtc gtttttcagc tggttcagta ccgtagtgat ctggtcaata tgacgaccat
    17161 ctggcgttgg ccaggcgcgc cactgtttac catacactgg tccgaggtcg ccgttttcat
    17221 cggcccattc gtcccagatg gtgacattgt tttcgtgtag ataagcaatg ttagtgtcgc
    17281 cctgcagaaa ccacagcagt tcgtggatga tggaacgcag gtggcaacgt ttagttgtca
    17341 ccagcgggaa tccatcctgc aggttaaaac gcatctgatg accaaaaatg gaaagcgttc
    17401 cggttccggt acggtcgttt ttctgtgtgc cttcgtcgag cactttttgc atcagttcta
    17461 aatactgttt catggttcct caggaaacgt gttgctgtgg gctgcgacga tatgcccaga
    17521 ccatcatgat cacacccgcg acaatcatcg ggatggaaag aatttgcccc atgctgatgt
    17581 actgcaccca ggcaccggta aactgcgcgt cgggctggcg gaaaaactca acaatgatgc
    17641 gaaacgcgcc gtagccaatc aggaacaagc cagagacagc tcccattggg cgcggtttac
    17701 gaatatacag gttgaggata ataaacagca ccacaccttc cagcagcagc tcgtaaagct
    17761 gtgatgggtg gcgcggcagc acaccgtaag tgtcgaaaat ggattgccac tgcgggttgg
    17821 tttgcagcag caaaatatct tctgtacggg agcctgggaa cagcatggca aacgggaagt
    17881 tcgggtcaac gcggccccac aattcaccgt taataaagtt gcccagacgc ccggcaccaa
    17941 gaccaaacgg aatgagtggt gcgataaaat cagaaacctg gaagaaggaa cgtttagtac
    18001 ggcgggcaaa gataatcatc accacgataa cgccaatcag gccgccgtgg aaagacatgc
    18061 cgccgtccca gacacggaac agatacagcg gatcggccat aaactgcggg aaattgtaga
    18121 acagaacata accaatacgt cccccgagga agacgccgag gaagcccgca tagagtaagt
    18181 tttcaacttc atttttggtc cagccgctgc ccggacgatt cgcccgtcgt gttgccagcc
    18241 acattgcaaa aatgaaaccc accagataca tcaggccgta ccagtgaagc gccacgggtc
    18301 ctattgagaa aatgaccgga tcaaactccg gaaaatgcag atagctactg gtcatctgtc
    18361 accacaagtt cttgttattt cgctgaaaga gaacagcgat tgaaatgcgc gccgcaggtt
    18421 tcaggcgctc caaaggtgcg aataatagca caaggggacc tggctggttg ccggataccg
    18481 ttaaaagata tgtatatgat ccgcgctata accctccgcg aatcagcccg cccatgccgc
    18541 gacgctccat aaaggctgca acctgatggc gaacttcggt cgccagttgc gcttccagac
    18601 tacgctgcgc aagattttct gcttcggcat aatcaatgcg ccgcagcagg tattttaccc
    18661 gcgctacaga acgtccgttc atagacagat ggcgataccc aagcccaatg aggattgcca
    18721 cgcacatggg atcgcccgcc atttcaccgc acaaacggag atcgattcca tgtatttccg
    18781 cttcccgggc gatcatcgcc agagctcgta acattgcagg atgaagactg tcataaatgt
    18841 tcgccacccg ggtattgttg cgatcaacgg ccagaatgta ttgagtcaga tcgttggtgc
    18901 caacagagat gaaatcgacc cgctttgcca gatgcggcag cataaatacc attgacggca
    18961 cttccagcat gatgccgata cgtggtttgg gaatttcgta accgatcatc tcctcgactt
    19021 cacgtccggc acgttcaatc aggcggcgtg cttcatcaac ttcatcgagg cttgtgacca
    19081 tcggcaacag aatattcagg ttgcccgtag cggcattagc acgcagcatc gcccgcacct
    19141 ggatcaagaa gatctccggc tgatcgagcg taatgcgaat cccacgccaa cccaggcatg
    19201 gattctcttc gctgatcggc atgtaaggca gctgcttatc tgctccgaca tccagcgtac
    19261 gcaaggtgac gggtttatca ttgaacattt gcagcatccc ctgatactgc gccacctgtt
    19321 cttcttccga cgggaaacca ctttgcagca tgaatgggat ttcagtgcga taaagaccga
    19381 tgccatcaat acggctgccc agtttttctt catgttccgg gcttaaacca gcattgagca
    19441 tgacttttat acgctcaccg ctttttaact gcgcgggtaa attgacgtca tcttccgcca
    19501 gacggctaag ctcaatctct tcactaatta gccgctgata ttcttgcagc agtaccggct
    19561 ccggatcgac cagcaattca ccgcgatagc catcaacgat cagcgtccta cgatgcagca
    19621 ccgaaggctg aatatccgcg cccatcacgg tagggatccc cagcgcacgt accatgatcg
    19681 cagcatggga gttggctgcg ccatctcgca cgacaacacc gactaagcga tcctggggca
    19741 gctcagcaag agttgtcgct gacagttcat ctgccaccag aatgaaacgt tccggccagg
    19801 cgttcggccc ttgattagcg tcatcaagat gaaacagcaa tcgctgaccc agcgcacgta
    19861 aatcgccagc ccgctctttg agatagttat cgcttagcgc ggcaaactgt tcggcaaatt
    19921 tttcaatgac cgtttttacc gcccactctg ccaccgagcc tttatcaacc tcggcaaaca
    19981 attcgcgacg cagccgggtg tccgaaagta ggtgcgagta aagatcgaaa atagccgccg
    20041 tttctttttg tgctccggcg gcaaagcgtt tgctgtagcg gcgaaactcg tttgccgctt
    20101 cttccagcgc cccggtcagt cgttcgcgtt ccagagccgg atccagcgtt gatgcctgat
    20161 acacctgttc cattaaaggt aacgtggcat cctgccagcc ttcggcaatc gccacaccag
    20221 gtgctgccgg taatgcgcgg attcgcgtct ggcgatattg cccaaacaag gcagtcaact
    20281 gcgactgaga aagaatagct gccatctggg tggcaagcgt caccaggaag gattcttcac
    20341 tttcgtcata ctggcgcaac tctcgttgct gtaccaccag tacaccaagc aactggcgac
    20401 gttgaataat tggtacgcct aaaaacgcgc ggaaacgttc ttcttttacg gaggggatgt
    20461 atttgaagct ggggtgcttt tgcgcatctg caaggtttat cggttccgcc agcctgccaa
    20521 ccaggccgac gatcccttca tcaaacgcga gcgttacagt gcgaccgcgt ggttttttca
    20581 gcccccgggt cgccatcagg tagtaacaac gtcgatcatg atcggccagg tagaccgaac
    20641 agacctcggt atccatcgca agacagatgt cggtaaccag aatatttaac gcctcattca
    20701 ggcgtggtgc gctggctacc ttttcgacta tttcgcgcag gcgagtgagc ataattggcg
    20761 tgacttaacc tcttttacgt cgataagcag atgtgttttg tggttttggc gtattttcct
    20821 gcagtgacat caccacactc gcgaactctt tcattaccct acggtagaca tcacgtttaa
    20881 atgacaccac ctgtctgacc ggataccagt aacttaccca tcgccagccg tcaaactctg
    20941 gtgtactgct ggtttgcata ttgatttctg catcgccgct caccagctgc aagagaaacc
    21001 atttttgttt ttggccgata caaaccggct tcgtgtccca acgcaccaaa cgtttcggta
    21061 atttgtagcg caaccagtta cgcgttgaag caaggattcg aacgtctttg cggcttaatc
    21121 ctacttcttc aaacaattca cggtacatcg cctgctctgc ggattctccg gggttgattc
    21181 cgccttgcgg aaattgccag gagtgctgac caaatcgccg ggcccacatt acctgcccct
    21241 ggcgattaca aatcacgata cctacgtttg ggcggtagcc atcgtcatca atcaccggac
    21301 tacctcaaaa taaagcttta tatacgaatg attgtttcat actccaagaa gacggtaaac
    21361 cactctctgc agggcattac acactaataa caattgaata actcacagtt atgtgcagag
    21421 ttataaacag aggaaggggt ggatagccgt ttttattcac cttttctgtg gatagagttg
    21481 tgaagaacta cggaattact acgggaaaac ccggagcatt ctgaataagc ccgatataga
    21541 cattttaaaa tattcattat tagtcaatgc attacatgta atttgagtaa tgaaaaagag
    21601 taattcgtga cccaggtcac acctctcatt tacgggttgg cgaaagatca accaatgccg
    21661 tatttatcca cagaatgtgc cactaagtta agcactgaac cactaaaaac tggagtttcg
    21721 tcgcacgtca aggctgtaaa tggaaacagt agtggaggtt tttcacagtt atcccagctt
    21781 tctgtggata acatggtgta agatcctgtt tattttcagt gaccagattt ggaaaaccta
    21841 ttgcagtgtt gcgcaactcg attaccggca gcctaaaaag caatataaat cagagaattg
    21901 aacaacacat gtggaaaaag ttacactgcg aatattcggc acataattgc tgtttgtttt
    21961 ttaaccaagg tatcatgaca tgtcccaacc tcgcccactc ctctctcctc ccgaaactga
    22021 agaacagttg ttagcgcaag cacagcaact ttctggttat acattgggag aactggcggc
    22081 acttgccggg ctggttacgc cagagaattt aaaacgcgat aagggctgga ttggcgtgtt
    22141 actggagatc tggctaggtg ccagcgcagg gagtaaacct gagcaagatt ttgctgctct
    22201 gggcgtggaa cttaaaacta tccctgtgga tagtcttggt cgtccgctgg aaacaacatt
    22261 cgtttgtgtt gccccgttaa cgggtaatag cggggtgacc tgggaaacca gccatgtgcg
    22321 ccacaagctc aaacgcgtac tgtggatacc ggttgaaggt gagcgcagca tcccgctggc
    22381 gcagcgtcgc gtaggatcac cgttgctgtg gagcccgaat gaagaggaag accgacagct
    22441 acgcgaagac tgggaagaat taatggatat gattgttctc ggtcaggttg agcggatcac
    22501 cgctcgtcac ggggagtatt tacagatacg accgaaagca gcgaatgcga aagcgcttac
    22561 cgaagccatt ggtgcccggg gcgaacggat tctgacgctg ccacgcggct tttatttgaa
    22621 gaagaatttc accagtgcac tactggcccg tcattttctg atccagtagc catcgctttg
    22681 acctgccgct ttccgggcat ataattaccg cttcattttt ttggcagggc tttttagatg
    22741 ttatttgcat ggataaccga tcctaacgcc tggcttgcgc tcggtacgct gacgctgctg
    22801 gagatcgttc ttgggatcga caatattatt ttcctttctc tggtggtggc aaagctcccc
    22861 acagcacaac gtgctcatgc gcgccgtctg gggttggcgg gagccatggt tatgcgtctg
    22921 gcgctgctgg catcaatcgc ctgggttacg cgcctgacaa atccgctttt tacaatattc
    22981 agtcaggaaa tttccgcccg tgatttgatt ctgcttctgg gtggcttgtt ccttatctgg
    23041 aaagccagca aggaaatcca cgaatccatt gaaggtgaag aagaagggct gaaaacacgc
    23101 gtttcatcat tcctcggcgc tatcgtgcag attatgctgc tggatattat cttcagcctc
    23161 gactcggtga ttaccgctgt gggtctgtca gatcacctgt ttattatgat ggccgccgtg
    23221 gtgattgccg taggcgtgat gatgttcgcc gcgcgctcga ttggtgattt tgtcgaacgc
    23281 catccttcgg taaaaatgct ggcgctctct ttcctgattc tggtcggctt taccctgatt
    23341 ctggaaagtt tcgacatcca cgtaccgaaa ggttacatct acttcgcgat gttcttctct
    23401 attgcggttg aaagcctcaa cctgattcgc aacaaaaaga acccgctctg atactccgta
    23461 cgctctcctg cgggagggcg tactcactcc tgttaccctc acagtttaag attttcctgc
    23521 tttcaagata tataacgtcg gtttataaac agactattat cataggtggg ccaaatgagg
    23581 ataaccggat gaaaaaatgg gcagtaataa tttctgcagt cggactggcg tttgctgttt
    23641 ccgggtgttc cagtgattac gtcatggcga ccaaagatgg ccgtatgatt ttgaccgatg
    23701 gaaaacctga aattgatgat gataccgggc tggtgagtta tcacgatcag caaggtaacg
    23761 cgatgcaaat taaccgtgat gatgtttcgc aaattattga acgttaacaa ataaggtcag
    23821 catccggctg taatgccgat cagttaag
//
LOCUS       DAZPBM010000049        23728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPBM010000049 DAZPBM010000000
VERSION     DAZPBM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.049"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..800)
                     /locus_tag="ACHUZQ_001816"
     CDS             complement(<1..800)
                     /locus_tag="ACHUZQ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1025527.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLA"
     gene            1451..1732
                     /gene="drpB"
                     /locus_tag="ACHUZQ_001817"
     CDS             1451..1732
                     /gene="drpB"
                     /locus_tag="ACHUZQ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1025528.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
     gene            1772..2428
                     /locus_tag="ACHUZQ_001818"
     CDS             1772..2428
                     /locus_tag="ACHUZQ_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR1025529.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2495..3913
                     /gene="dcm"
                     /locus_tag="ACHUZQ_001819"
     CDS             2495..3913
                     /gene="dcm"
                     /locus_tag="ACHUZQ_001819"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1025530.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3894..4364
                     /gene="vsr"
                     /locus_tag="ACHUZQ_001820"
     CDS             3894..4364
                     /gene="vsr"
                     /locus_tag="ACHUZQ_001820"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1025531.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4353..5274)
                     /gene="yedA"
                     /locus_tag="ACHUZQ_001821"
                     /pseudo
     CDS             complement(4353..5274)
                     /gene="yedA"
                     /locus_tag="ACHUZQ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5447..6364
                     /locus_tag="ACHUZQ_001822"
     CDS             5447..6364
                     /locus_tag="ACHUZQ_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1025532.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6443..6625
                     /locus_tag="ACHUZQ_001823"
     CDS             6443..6625
                     /locus_tag="ACHUZQ_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1025533.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6796..8490
                     /gene="dgcQ"
                     /locus_tag="ACHUZQ_001824"
     CDS             6796..8490
                     /gene="dgcQ"
                     /locus_tag="ACHUZQ_001824"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR1025534.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8487..9302)
                     /locus_tag="ACHUZQ_001825"
     CDS             complement(8487..9302)
                     /locus_tag="ACHUZQ_001825"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR1025535.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9600..9827)
                     /gene="yodD"
                     /locus_tag="ACHUZQ_001826"
     CDS             complement(9600..9827)
                     /gene="yodD"
                     /locus_tag="ACHUZQ_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR1025536.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9990..10178
                     /gene="dsrB"
                     /locus_tag="ACHUZQ_001827"
     CDS             9990..10178
                     /gene="dsrB"
                     /locus_tag="ACHUZQ_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1025537.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10222..10845)
                     /gene="rcsA"
                     /locus_tag="ACHUZQ_001828"
     CDS             complement(10222..10845)
                     /gene="rcsA"
                     /locus_tag="ACHUZQ_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR1025538.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11130..11915)
                     /gene="fliR"
                     /locus_tag="ACHUZQ_001829"
     CDS             complement(11130..11915)
                     /gene="fliR"
                     /locus_tag="ACHUZQ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1025539.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11924..12193)
                     /gene="fliQ"
                     /locus_tag="ACHUZQ_001830"
     CDS             complement(11924..12193)
                     /gene="fliQ"
                     /locus_tag="ACHUZQ_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1025540.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12203..12940)
                     /gene="fliP"
                     /locus_tag="ACHUZQ_001831"
     CDS             complement(12203..12940)
                     /gene="fliP"
                     /locus_tag="ACHUZQ_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR1025541.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12940..13305)
                     /gene="fliO"
                     /locus_tag="ACHUZQ_001832"
     CDS             complement(12940..13305)
                     /gene="fliO"
                     /locus_tag="ACHUZQ_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1025542.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13308..13721)
                     /gene="fliN"
                     /locus_tag="ACHUZQ_001833"
     CDS             complement(13308..13721)
                     /gene="fliN"
                     /locus_tag="ACHUZQ_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1025543.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13718..14722)
                     /gene="fliM"
                     /locus_tag="ACHUZQ_001834"
     CDS             complement(13718..14722)
                     /gene="fliM"
                     /locus_tag="ACHUZQ_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1025544.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14727..15191)
                     /gene="fliL"
                     /locus_tag="ACHUZQ_001835"
     CDS             complement(14727..15191)
                     /gene="fliL"
                     /locus_tag="ACHUZQ_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1025545.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15296..16423)
                     /locus_tag="ACHUZQ_001836"
     CDS             complement(15296..16423)
                     /locus_tag="ACHUZQ_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1025546.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16420..16863)
                     /gene="fliJ"
                     /locus_tag="ACHUZQ_001837"
     CDS             complement(16420..16863)
                     /gene="fliJ"
                     /locus_tag="ACHUZQ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR1025547.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16882..18255)
                     /gene="fliI"
                     /locus_tag="ACHUZQ_001838"
     CDS             complement(16882..18255)
                     /gene="fliI"
                     /locus_tag="ACHUZQ_001838"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1025548.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18255..18941)
                     /gene="fliH"
                     /locus_tag="ACHUZQ_001839"
     CDS             complement(18255..18941)
                     /gene="fliH"
                     /locus_tag="ACHUZQ_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1025549.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18934..19929)
                     /gene="fliG"
                     /locus_tag="ACHUZQ_001840"
     CDS             complement(18934..19929)
                     /gene="fliG"
                     /locus_tag="ACHUZQ_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1025550.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19922..21580)
                     /gene="fliF"
                     /locus_tag="ACHUZQ_001841"
     CDS             complement(19922..21580)
                     /gene="fliF"
                     /locus_tag="ACHUZQ_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR1025551.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21796..22110
                     /gene="fliE"
                     /locus_tag="ACHUZQ_001842"
     CDS             21796..22110
                     /gene="fliE"
                     /locus_tag="ACHUZQ_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1025552.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            complement(22135..22263)
                     /locus_tag="ACHUZQ_001843"
                     /pseudo
     CDS             complement(22135..22263)
                     /locus_tag="ACHUZQ_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            22444..22776
                     /gene="emrE"
                     /locus_tag="ACHUZQ_001844"
     CDS             22444..22776
                     /gene="emrE"
                     /locus_tag="ACHUZQ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1025553.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22945..23496
                     /gene="ybcL"
                     /locus_tag="ACHUZQ_001845"
     CDS             22945..23496
                     /gene="ybcL"
                     /locus_tag="ACHUZQ_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1025554.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23506..>23728
                     /locus_tag="ACHUZQ_001846"
     CDS             23506..>23728
                     /locus_tag="ACHUZQ_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1025555.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
BASE COUNT         5759 a         5945 c         6139 g         5885 t
ORIGIN      
        1 attgcaagat aaacattgtt ggcgtcgtat tttgcaccaa atgtccatgc ttcagctgta
       61 tcaccaccag caaatttgct gtattcattg tagtgatacc cgccaagagc tacttgagaa
      121 ttggtacggt cagagtttga gtaagcggca cctaagctca aaccgaagtc aaagtcatat
      181 gaagtggaaa gaccaaaacc atcaccgttc tggaagcgaa cgtcttcgtg accattttta
      241 gtaccttctt gatcttctcc tgcattgctg ccttcgttgt taccttgata ctgcaatgca
      301 aagttcagac cttccaccag accgaagaaa tcactattgc ggtaagtagc taccccattt
      361 gcacggcctg ccatgaagtt atcagtccag gtataggagt cgccgccgaa tacaggaagc
      421 atatctgtcc aggcttcaac gtcgtaaatc acaccatagt tacgaccata gtcaaaggag
      481 cccatctcgc cgaacttcag acctgcaaaa gcaagtttga cataagtatt atcagaacct
      541 tcagtgtcgc tagcctttgt tttggtcagt gcttgaccat aaccagttaa ctgatctgta
      601 atttgggtct cacctttgaa gcctaactcg atgatagttt catcgccgtc atcgccaggg
      661 ttatcggaaa aagtatgacg agcatcaact tttccgtaca gatccagttt attgccattt
      721 ttattataaa tttcagccgc atttgctgcg ccagcaacta ataacgctgg gaccagcatt
      781 gccagaactt ttcttttcat tatgtattcc cttgtgatta taatcttcat gaatatatca
      841 ataagtgccg ttatccaaaa aaaaagcaca tttggatact attctatgaa gttcatttta
      901 ttttaaagat tgcgtgtaac aaatgtattt aaaagatttc aaaatgtttc taatctattt
      961 aatataattc agaattatga attctaaata taaacatcca tgaaaattat aaatgtatat
     1021 caatatgtta tgagttgaat ggaaagatag ggagtatcaa tgattgttgc gccaaacggc
     1081 aggcgtgatc acgcgcacat taatgaaggt ataaaaaaag ccggcatcat gccggcccaa
     1141 aaaatataaa caagacgata aaaggctaat aacggggtta tcatgacaca gaaatcaaaa
     1201 aaggtataat tgtattcagt gctaatatat tgtttgggtg aattaataaa actattacca
     1261 aggatataaa taaatcttta ttgctgtttt tccagtaatt attgccgttg cgcaaaattt
     1321 atctatttgt tcaaaaaatg attgagtctt gactggctca tccaatgtgg aaaaatgtga
     1381 cttttatcac ataatagtac taagtctgaa ttttccgggt tatctcaaaa tggaatacgg
     1441 ttcgacaaag atggaagaga gactctctcg cagccctggc ggcaaattgg ctttgtgggc
     1501 tttctataca tggtgtggct atttcgtctg ggcaatggcg cgttacatct gggtgatgag
     1561 ccggatccct gatgccccgg tgtcgggatt tgaaagcgat ttaggctcta ccgcgggaaa
     1621 gtggttaggg gcactggtcg gatttttatt tatggctctg gtcggggcat tgttaggcag
     1681 cattgcctgg tacacccgac ctcgtcccgc acgtagcaga cgctatgaat aaaaactgct
     1741 aaggtggcga ctctttggca acggagcgcg tatggactta caacactggc aggcacagtt
     1801 cgaaaactgg ttgaaaaacc atcatcagca ccaggacgcg gcacagaagc tggcggcaga
     1861 tgacgatgtc gatatgctgg tgattttaac cgcctgttat tttcacgata ttgtgagcct
     1921 ggcgaaaaat catccgcaaa ggcagcgttc ttcaatcctg gcggcagaag agacgcgtcg
     1981 cctgctccgt gaagagttcg agcaatttcc ggcggagaaa atcgaggccg tttgtcatgc
     2041 cattgccgct cacagtttca gcgcgcaaat cgccccctta acaacggaag ctaaaatcgt
     2101 ccaggatgcg gatcggctgg aagccctggg ggcgattggc ctggcgcgcg tgtttgccgt
     2161 ttcaggggca ttgggcgtgg cgctgtttga tggtgaagat ccgttcgcac agcatcgccc
     2221 gcttgatgat aaacgctacg cgctggatca tttccagact aagttgctga agctaccgca
     2281 aaccatgcaa accgcaagag gcaagcagct ggcgcagcac aacgcgcatt ttttagtcga
     2341 gtttatggcg aagctcagtg ccgaactggc gggggagaat gaaggtgtcg atcacaaggt
     2401 gatagatgcg ttttcacccg cgggataagc gcgtggccct aaatggctgt aattatgtta
     2461 acctgtcggc catctcagat ggccggttaa atctatgcag gaaaatatat cagtaaccga
     2521 ttcatacagc accgggaatg ccgcacaggc aatgctggag aaactgctgc aaatttatga
     2581 tgttaaaacg ctggtggcgc agcttaatgg tgtgggtgag aatcactgga gcgcggcaat
     2641 tttaaaacgt gcgctggcga atgactcggc atggcaccgt ttaagtgaga aagagttcgc
     2701 ccatctgcaa acgttgttac ccaaaccatc ggcacatcat ccgcattatg cgtttcgctt
     2761 tatcgatcta tttgccggaa ttggcggcat ccgtcgcggt tttgaatcga ttggcggaca
     2821 atgcgtgttt accagcgaat ggaacaaaca tgcggtacgc acttataaag ccaaccatta
     2881 ttgcgatccg gcgacgcatc attttaatga agatatccgc gacatcaccc tcagccataa
     2941 agaaggcgtg agtgatgagg cggcggcgga acatattcgt caacacattc ctgaacacga
     3001 tgttttactg gccggtttcc cttgtcagcc attttcgctg gctggcgtat cgaaaaagaa
     3061 ctcgctcggg cgggcgcacg gttttgcctg cgatacccag ggcacgctgt tttttgatgt
     3121 ggtacgcatt atcgacgcgc gtcgtccggc gatgtttgtg ctcgaaaacg tcaaaaacct
     3181 gaaaagtcac gaccagggta aaacgttccg catcatcatg cagacgctgg acgaactggg
     3241 ctatgacgtg gctgatgcag aagataacgg gccggacgat ccgaaaatca tcgatggtaa
     3301 acattttctg ccgcagcacc gtgaacgcat cgtgctggtg ggttttcgtc gcgatcttaa
     3361 tctgaaagcc gattttactc tgcgtgatat cagcgaatgt ttccctgcac agcgagtgac
     3421 gctggcgcag ctgttggacc cgatggtcga ggcgaaatat atcctgacgc cggtgctgtg
     3481 gaagtacctc tatcgatatg cgaaaaaaca tcaggcgcgc ggtaacggct tcggttatgg
     3541 aatggtttat ccgaacaatc cgcaaagcgt cacgcgtacg ctgtctgcgc gttattacaa
     3601 agatggcgcg gaaattttaa tcgatcgcgg ctgggatatg gccacgggtg agaaagattt
     3661 tgacgatccg ctgaatcagc aacatcgtcc acgtcggtta acgcctcggg aatgcgcgcg
     3721 cttaatgggt tttgaagcgc cgggagaagc gaaattccgc attccggttt cggacactca
     3781 ggcctatcgc cagttcggta actcggtggt cgtgccggtc tttgccgcgg tggcaaaact
     3841 gcttgagcca aaaatcaaac aggcggtggc gttgcgtcag caagaggcac aacatggccg
     3901 acgttcacga taaggccact cgcagcaaaa atatgcgcgc gattgccacg cgtgatacgg
     3961 cgattgagaa gcgcctcgcc agtctgttaa ccgggcaggg cctggcgttt cgcgttcagg
     4021 acgccagtct gcccggaagc ccggattttg tcgttgatga atatcgctgc gtgatattta
     4081 cccatggctg cttctggcat catcatcact gctatctgtt taaagtgcct gcgactcgaa
     4141 ccgagttctg gctggagaag ataggtaaaa atgttgaacg cgatcgccgc gatatcagtc
     4201 gtttgcagga actcggctgg cgcgtactga ttgtctggga gtgtgcatta cgtgggcgcg
     4261 agaagctgac ggatgaagcg cttaccgagc gtctggaaga gtggatctgc ggcgaaggtg
     4321 ccagcgcgca gatcgacacg caggggattc atttactcgc ttgatgcgtc ctgaataact
     4381 ggcgcaacta cgggttttgc cgggaagaga tattttccca acgtgaccag taccaccgcg
     4441 aagacaatta cgccgagcgc cagccattca atcttcgaca gcgtctctcc gcccagtccc
     4501 gtacccagca agaccgcgac caccgggtta acgtaggcgt agctggtggc gagagccgga
     4561 ctgacattac ggattaaata catataagcg ttgatggcga taatcgaacc aaacagcgcc
     4621 agatagccga ccgcaaggaa gcctgaaagg gaagggagcg ccgtcagttt ttcacctgca
     4681 atcatcgacg cgatcattaa caccacgcct gccgccagca tctcaatcgc acccgccatc
     4741 atccctacag gtaaggtaat gcgcgagcca taaaccgagc caaacgccca gctaatcgag
     4801 ccgattaaaa tcagaatcgc gccccacgga ttgccgctta aatttccacc gctattgagc
     4861 atgatgattc cggcaagccc aatggcgata cccacccatt ccagtttgcg cgttttaatg
     4921 ccaaacaggc ggctgaagca ccagggtaaa gaggggcacg gttgcaacca caacggcggc
     4981 gatgccggaa ggaacatttt gatgctcggc aaccgtcacc atgccattac cgacagccag
     5041 caataacagg ccaatcagcg cggcattgag cagcggacgt agcgggggga gcttgtgtcc
     5101 gcgcagtagc aaaaatgcca tcaataaaat accggctgcc aggaatcgaa cgcctgccat
     5161 cattaacgga ggccagcttt ccacgccaat ccgaatgaca aaataggttg agccccaaat
     5221 gatatacaac gcaaacagcg cgccaaaaag cggtaacaac tggcggaaac gcataatccc
     5281 tcacggtgga aataaaaagg tggttcatag taaacgtgaa aatcattctg ctggcgagag
     5341 atataattgc acttgattgt aaaaaaaatg ttgacctgtg aagcaagtat cagagctgcc
     5401 gtttttgctt catacttaca cccttcaaca ataaaaatga gagggaatgc ttttggccgg
     5461 gagtagttta ctgacgttgc tcgatgatat cgccacacta ctggacgata tctccgtgat
     5521 gggcaaactg gcggcgaaga aaaccgccgg tgtattaggg gatgacttat cgctcaatgc
     5581 gcaacaagtt tcaggcgtgc gggccaaccg ggaacttccc gtggtctggg gcgtggcgaa
     5641 aggatcgctg attaataaag tgattctggt gccgctggcg ctgatcatca gtgcgtttat
     5701 cccgtgggcg attacgcctc tgttgatgat tggtggcgcg tttctctgct ttgaaggagt
     5761 agagaaagtg ctgcatatgc tggaggcgcg taaacataaa gaagatccgg cgcagagcca
     5821 gcagcgtctg gagaagctgg cggcgcagga tccgctgaag tttgaaaagg acaaaataaa
     5881 aggggcgatt cgtaccgatt ttatattgtc tgccgaaatc gtcgccatca cgctggggat
     5941 tgtggccgaa gcgccgttgc ttaatcaggt gctggtgctt tcaggcatcg cgctggtagt
     6001 gaccgtgggc gtctacggtc tggtaggggt tatcgttaag attgatgacc tggggtattg
     6061 gctggcggaa aaatccagcg cgctgatgca ggcattaggt aaagggttat tgatcattgc
     6121 gccctggttg atgaaggcgt tatcgattgt cggcacgctg gcgatgttcc tcgtcggcgg
     6181 cggtattgtg gtacatggta ttgcgccgct gcatcacgcc attgaacatt tcgccgggca
     6241 gcaaagtgca gtggtggcga tgatattacc gactgtttta aatctgattc ttggatttat
     6301 catcggcggc atcgtggtgc tgggagtgaa agccgtagcg aaaatgcgcg gtcaggcaca
     6361 ttaatactcg tacaggctat tatgcaaaat tcgccatctt caactaaggt gaacaagttt
     6421 cactcgaaaa aaggaggcga gtatgagctt tatggttagt gaggaagtta cggttaaaga
     6481 gggcggcccg cggatgattg tcactggata ctccagcggt atggttgagt gtcgttggta
     6541 tgacggttac ggggtcaagc gggaagcttt tcatgaaacc gagcttgttc cgggggaggg
     6601 gagtcgttct gcggaagaag tttgaaaggg tatcattaac accaggccgg atcacttccg
     6661 gcctggtgtg ccaatgcctg caatgaatga tagtcgcgtc ttatcatgcc accattataa
     6721 aattatcgct gttttgcggt acgctatcgc caggttatgc cagaatcata aaaaagcagg
     6781 ttgggagtcg tcagggtgca gcacgagaca aaaatggaaa accagagctg gttgaaaaaa
     6841 ctcgcacgcc gcctggggcc tggtcatgtc gttaatctct gctttatcgt ggtattgctt
     6901 ttttccacct tgctgacctg gcgtgaagtg gtagtgctgg aagatgccta tatctccagc
     6961 cagcgtaatc atctggaaaa cgtagccaac gcgctcgata agcatttgca gtataacgtc
     7021 gacaaactga tctttttgcg taatggcatg cgcgaagctc tcgtagcgcc actggatttc
     7081 acttcactgc gtaatgctgt aaccgagttc gaacagcatc gcgacgagca cgcctggcaa
     7141 attgaactca accgacgacg caccctgtca gtcaatggcg tatcggatgc attagtcagc
     7201 gaggggaatc tcctgtctcg cgaaaatgaa agcctcgaca atgaaattac cgctgcactg
     7261 gaagttggtt acttgctgcg actggcgcac aacacctcgt cgatggttga acaggcgatg
     7321 tatgtctcgc gtgccggatt ttacgtttcg acgcagccga ccttgtttac gcgcaatgta
     7381 ccaacgcgtt attacggcta tgtcacccaa ccctggttta tcggccattc gcaacgagaa
     7441 aatcgtcacc gcgcggtacg ctggtttact tcgcaaccgg aacacgccag caatactgaa
     7501 ccgcaggtta ccgtcagtgt tccggtagac agtaataact actggtatgg cgtgctgggg
     7561 atgagtattc ccgtgcgtac catgcagcaa tttttaagaa acgccatcga taaaaacctc
     7621 gatggtgagt atcagctcta tgacagtaag ctgagatttt tgacctcttc caatcctgac
     7681 catccaacag ggaatatttt tgatcctcgt gaactggcct tgctggcgca ggcgatggaa
     7741 catgacacgc ggggcggcat tcgtatggac agtcgctatg ttagctggga acgtctggac
     7801 catttcgacg gtgtgctggt gcgcgtccat acgctaagcg aaggcgtgcg cggcgatttc
     7861 ggcagtatca gcattgcatt aaccctgctg tgggcgctct ttaccaccat gttactcatc
     7921 tcctggtatg tgattcgccg gatggttagc aacatgtatg ttctgcaaag ctcgttgcag
     7981 tggcaggcgt ggcacgacac cttaacccgt ttatataatc gtggcgcact gtttgaaaaa
     8041 gcccgtccgc tcgcgaaatt gtgtcagacg caccaacatc ctttttctgt cattcaggtc
     8101 gaccttgacc attttaaagc gattaatgac cgctttggtc atcaggcggg cgaccgtgtt
     8161 ctttctcatg ctgccggatt aattagcagt tccttgcgtg cgcaggacgt tgccgggcgg
     8221 gtcggtggtg aggagttttg tgtgattctg ccaggcgcga gtctgacgga ggctgcggaa
     8281 gtcgcagaac gtattcgcct gaagttaaat gaaaaagaga tgttgattgc taagagtacg
     8341 acgatacgca tcagtgcctc gttgggggta agtagcagcg aggaaaccgg tgattatgat
     8401 tttgaacaac tccagtcact ggctgaccgt cggctttatc tcgctaaaca ggccgggcgt
     8461 aatcgggtat gcgcgagcga taacgcttaa cgagcggaga aaaaatggtc cagcccttca
     8521 cgccagcctt ccggtccttc acgctgcgtt cgccagacgc gggtcggatc ctcatcatgc
     8581 agatgcaccc cttcacggtt tagccctttc acaatcaccg cgtaatccat tacctccagt
     8641 aagggcgcat cgtttggccc atcgcccagg ccaagtgtgg ttgggcgttt gcctgataat
     8701 tgttgatagg tcgcgataat ccagttggcg gcctgatctt ttccggcaga ggcatccagg
     8761 acgtgccaga agcgcgcacc ctgcataaac tgtaagccca gttcgttcag acgagcggta
     8821 aattgtgcca tacgctcgtc actgtcgcgc cagattagcg ttaccgacgc ctcatgcagc
     8881 tgcgtcagcg ccgcctggct acggcttaat cccgtccatt cggcgatggt tgcatcgtcg
     8941 acatcatcga aagtcgtgaa tttaaaatgt tctttctcgc gtagcgtatt taaaacctgg
     9001 ctgatttcgc catggctaat acctgagatg atgcgtggaa aaccgtctat atcctgccat
     9061 tgttcagcaa gctggatcac tgcgccattt tcggcaatca gtggtaaccc ttgtagcccc
     9121 aacgtttttt gcaagtacag catttccgct gaagttttac tgctacagag aatgacggga
     9181 acattcgctt cgcgtaaacg gctgagccag agggcagtcg gttgccagtc ataactatga
     9241 ctgtccagca gggtgccatc aagatcgcta aaaaccagta gtggttgttg aattgaaaac
     9301 atggtcgtgc agttctcccc ttaccaggat gattctcaaa acagcacaaa taatcaaaga
     9361 tgattttttc gggagcgatc ccgttgacgc cagagccacc aaggggtgac gcacgtcaca
     9421 tttctattca taagtagcgt taattattca tatcgcgaat attttcttgt cagcgaaaaa
     9481 aattgcggat aaggtgatga acacatcaga tttcctggtg taacgaattt tttaagtgct
     9541 tcttgcttaa gcaagtttca tcccgacccc ctcagggtcg ggattttttt attgtgcatt
     9601 caacgattca cttcagagac gctgaagtca tgaatatcca gttcgaaggc atccgccaat
     9661 tcacgccatg tatgatattc acgtccatcg acgctaacgt gttcagaatc gttctggctg
     9721 cgatgaactt cgctttcgct aatttcattg atcgccgcca gcagggcatc gacatcgacg
     9781 ctgacctcac gttttgcggt atcgctgtac tcttttgcgg ttttcatcat ctttcctcgc
     9841 aaccgttact ctgttgttgc cgccagtcgt taagtccgcc tggcatctct taagtataga
     9901 ccgtcgagaa aatcagcact cgcgcgcgcc tggagctgcc agaaaaacga aattgttcta
     9961 cactggcaca aagccacagg aggaaaacga tgaaggtgaa tgatcgggta acagtcaaaa
    10021 cggatggcgg tccgcgtcgt cctggcgtgg tactggcagt tgaggagttt agtgaaggca
    10081 caatgtacct ggtttcgctg gaagactacc cgctcggcat ctggttcttt aatgaagcag
    10141 ggcatcagga cggtatcttt gtggagaaag cagagtaatc ttattcagcc tgactggtgg
    10201 gaaaccacca gtcagaatgt gttagcgcat gttgacaaaa ataccattag tcacattatc
    10261 cgtcagtcgg acgacatggt agataacctg tttattatgt gttttgatct tacgtttaat
    10321 attaccttta tgcgatgaaa cggtcttggc tttgatattc atttggtcag agatttgaat
    10381 ggttccctga cctgccatcc acattcgcaa catactcgat tcggttcggc tcaatgataa
    10441 cgtcggcata tttaaaaaag aggttatcgt tgtctctttt ttcagaatat cgccaaggat
    10501 atcgtcgaga gattccggtt taatcgattt agaacttatc aataaatttt ttctgaccaa
    10561 tagatattca tcaaaatgaa cattggcaat tgccataaaa acgataaata acgtattggg
    10621 atgttgatta atgatgtgct tgatgtgctg actgttagaa gcatcatgga tgaaacagtc
    10681 ctcattaata aacaccactg aagggcgctg tgaatcacaa gctatggcaa ggtcatcaac
    10741 ggtttcaatg tcgttgattt ctcttttttt aacccctcta ctcaacagat acccggttaa
    10801 acctagtcgg gtgtaactac ataaatccat aataatcgtt gacatggcat accctcactc
    10861 aatgcgtaac gataattccc cttacctgaa tatttcatca tgactaaacg gaacaacatg
    10921 ggtcacctaa tgcgccactc tcgcgatttt tcaggcggac ttactatccc gtaaagtgtt
    10981 gtataatttg cctggaattg tcttaaagta aatgttgcga tatgtgagtg agcttaaaac
    11041 aaatatttcg ccgcaggagt atcctggaag atgttcgtag gtattatttt tcggataatc
    11101 cttaggataa catgataaac gttacggaat tatattaatg gcaattcact aataatatca
    11161 gccagcaaat taaacatctc actgaataaa tgttcgcaaa aaggtgcaat taacggcatt
    11221 aatgccgcca ttaaagagat accgacagtc agagttaatg gaaatccaat aacaaaaatg
    11281 gataattgcg gggccatacg attcagtaaa cctaatgcca gattcagggt cagcagcaga
    11341 gtaatgagcg gtaacgccag catcagcccg ttaaggaaaa tcaaactccc cgctttggtg
    11401 agtgccagaa acgcattgct gttcaacggt tcgccaccaa ttggcagggt gtgaaaggta
    11461 tcgaccagca gtgaaatcaa ccataaatga ccgttaaatg tcaggaacag cagtaacgcc
    11521 agcatatcca tgatacgcgc taaaacgggc atattgagat ggctggccgg atcgacaaac
    11581 gtcgcaaatg acagccccat ttgcagaccg ataatttcgc cagcggttcg cacagcggca
    11641 aaggcaaatt gcatggtaaa accaagcgca atgccgatca ggatctgctg cacggccagc
    11701 cacagagcaa agaacgaaaa aacaggaaca tcgttggcag gtaatgatgg ggcaatgacg
    11761 aacgtgatca tcattgccag ccccagtttt acccgtttcg gcacgctgcg ttcgctcaga
    11821 attggtgcgg tagaaatgag cgccagcacg cgcagtaacg gccagaagta caggcttaac
    11881 caggaaagcc attgatcgct tgtcacctgc atcattgtac ggcctacccg atgatatacg
    11941 gcaggttagt gaacaaggtg cggacgtaat ccagcaacag attgagcatc cacggtccgg
    12001 caataataat ggcaataaat acggcgatga tttttggaat aaacgacagc gtcatttcgt
    12061 tgatctgcgt ggcggcctgc aaaatactga tgataaggcc cgtgaccagc gccaccagca
    12121 acagcggggc agccagtgcc agcgcgactt tcatcgcttc agtccccatc atcatgaccg
    12181 attcaggtgt cattttgcct ctctagctgt aaaagctctg cgccagcgaa ccgaccagca
    12241 attgccagcc atccaccagt acaaacagca tcagtttaaa gggcagagca atggtggcgg
    12301 gggggaccat catcatcccc aatgccatca acacgctggc aatcaccagg tcgataatca
    12361 aaaaaggaat gaaaatcgtg aagcctatct gaaatgcggt tttcaattcg ctggtcacgt
    12421 aggccgggag caaaatgcgc atcggcacgg cttcaggtcc ctgcaacggg ccggtattcg
    12481 ctagtctggc aaacaaccct aaatctgcct cacgggtctg acgcagcata aactcacgta
    12541 gcggctgcgc ccctttttcc agcgcctcct gcattgatat tttctcttcg ctgaatggct
    12601 ggtacgcatc gacataaatt ttgtcgatca ccgatgacat aataaaaaag gtcaaaaata
    12661 gtgccagccc cagcaatacc tggttaggtg gcgcggaggg cgttcccagc gcgttacgca
    12721 ataaaccaaa aacaatgatg atgcgggtga aactggtcat catcagtaaa attgccggaa
    12781 taaacgtcaa cgaggtgatg aacaccagcg tctgcaccgg gagcgaccag ctttgtccac
    12841 cgccaggcag cggctggctg gtgatacccg gcagttgcgc gaaggcgagg ggcgtaacca
    12901 gccagagaag gacaggtgcg acagacaaca aacgacgcat caggatctcc cactacgctt
    12961 aagcaaattt ttcatgaccg actgaaaatc ggtctgcggt atctcttctg ttggtgctga
    13021 agggggaagt ttatgcagca gattgatttg ccctgcggta acgccgagca ccagccgtgc
    13081 atcttccaca tcgaccacca caacccgttc acgcgcgccc agcgaagcac tggcgctaat
    13141 tttcagaccg ttaacgccag tgcgtttcgg ggcaaatccc aaccgtttta ccagccaggc
    13201 agcagcgagg atcagggcaa taatggcgat gagtgcgccg ctgacctgca gcagtggcgc
    13261 agcagaaacc ggcgtggaag attgcacagt agcgtggtta ttcatcacta acggctcagg
    13321 cgacgcattc gctcagacgg agtaatgata tcggtgatcc gcacgccata tttatcggca
    13381 acgaccacca cttcgccctg ggcgattaaa taaccgttga tcagaatatc cagtggttcg
    13441 cccgccagac cgtccagcgc cacgacggac ccttgcgtca gacgcaacag ctctttgatg
    13501 gtcatccgcg tacggcccag ctcgacggtc agcttgaccg gaatatccat tatcaggtcg
    13561 atatcctgca acgttccgct gacatcacca ccgccaaatt gctggaacac cgcgtcggca
    13621 gcgcttttgc tgctggttga tttttgttcg ctcaacgctt cagcccacag atcgtccatt
    13681 gcgccgttgt tgtcatcggc cggattattc atgtcactca tttgggctgt tcctcgttca
    13741 gagaatttaa aatcgggtta atcaaatgtt ctatccgtaa cgcatactga ccgttgagag
    13801 tgccgtactg actggtcagc accgggacgc cgtcaacatg ggcgatgatg ccatcgggtt
    13861 tttctatcgg caggacgtcg ccgggtttca gttttaaaat ctgcgacagg cgtagcgaga
    13921 tatcggcaaa gttggcgacc agctccagct gtgaatgctg cacctggcgc accaggttat
    13981 cgcgccagtt ctgatcttca ttacgcgagt tttccagcgg cgggttaacc aacaattccc
    14041 gtagcggctc gatcatgctg aatggcagac agatattaaa ttcgccggtc aggttgccaa
    14101 tctccacatg gaacggcgtg ttaaccacaa tgtcgttcgg cgaggtggtg atattggtaa
    14161 atttcacctg catttccgaa cgcacgtact caacttccag cggattaatc gccttccagg
    14221 cgtcgctata gccttcaagc gccagcttta acatgcggtt gatgacgcgc tgttcggtat
    14281 gggtaaactc gcgaccttcc actttggtcg ggaagcgtcc attgccgcca aacaggttat
    14341 ccacggcgat aaacaccaga ctcggtgaaa acaccaccag ccctgtgccg cgcagcggtt
    14401 tcagatggat aaggttcagg ttggtcggca ccggcaggtt gcgggcaaat tcatggtacg
    14461 gctgaatgcg gatggccccg acggttatat ccgggctacg acgcagcagg ttgaacagcc
    14521 ccatacgaaa atggcgggca aagcgctcat taatgatttc cagcgcctgc aaacgttcgc
    14581 gcacaacccg tcgttgggta ttcggatcgt acggacgaat gtcactttcg ccgctaacac
    14641 tggctgtcgg ttcgtctttg acttcgctgt caccattcaa cagcgcatca atttcagctt
    14701 gagaaagaat actatcgccc atgtcgttat cgcagaataa aagcggtata catcacgtcg
    14761 gtgacatcct gtttcggttg cccggcaaca agcggggtgg aaagcgtggt tttaatctcg
    14821 gcaatcaggt ttttcttgcc ttcttctgtc gccagtacgg cagcatcctg acgcgaaaac
    14881 agtaacagca aacggctacg gacttccggc aaatactcac tcagccgcga gcgggtagct
    14941 tcatctttga gtcgcagggt tataccgata taaagcacgc gatccgcatc gcccaaattg
    15001 accgtaaagg tatccagcgc atagaagacc ggtgagggca cgacgcgatg ctgcgctttg
    15061 tcttcagcgg caacctgatg cgaatgccag tagctgtaac ctgcgctggc acaggccgcg
    15121 agggtaatga ataccagaat cgggagccaa agcgatcgct tgcttttctt gcttatcgcg
    15181 taatcagtca tgtgttgcgg tcttcctgtg tcgctactgc ttatcggcta attatcctgc
    15241 gtcttgagcg caacaaagcg gggaaaagac gcggattacg gtgctacctc tgacgttagg
    15301 cgaaaatatc aacgccgctg ttgcctgtta cgcgcccttg taaagagacg ggaaccggaa
    15361 gcgtatcgtc ttcttccccc gccagaggtt catggtttac tgtgcgttgg ctttgctgtt
    15421 gttgggaagc ggcctgctgc tgaccactaa agctttcgcc actgatgttg ctttgcccta
    15481 actgaatgcc actctcggcc agctgcgtac gcagtaccgg cagcgctgct tccagggcgg
    15541 cgcgtacgtg ctgatgcggc gaaaccatct ggatttgcgc ctggttatca tccactttga
    15601 gggagatttg cacttcacct aaatcctgcg ggtgcagacg caactctgca ctttgttgtc
    15661 cctggcgggt gaacagcgaa atatgctggc ttaatgattg ttgccattcg tgagaaccca
    15721 gcggtgcact caagacaggc gcggcgacgg tgagcagtgg ctgtgtctgg tgtggagtga
    15781 ttagcgggct ggcggcagcg gtcaccggtg aaggtgtgct gatgacttcc gctttactct
    15841 gggcttctgc taccagcggt gttaatggct gagctggcgt gccgggggca tcatcaggct
    15901 gtgctgttgt gagttgcgca gaagtcagtt ttgtgaagag cgttggtttc tctgccggta
    15961 acacggttga cggcgcatca gtcactttgg gcgtattgtc aaaacccggc aacatcgcaa
    16021 aaagggcgct caggcttgcg gtgaggtctt cattcagatc atccgctttt tcgtcttttg
    16081 tcgtattttt gtcagccacc gcagccaacg tcatcgtatg agcggtggtt aacggtgtcg
    16141 atgtggattg ttcgacgttg atgacaggca gtgtttcatc cacagggatc agtaaatccg
    16201 cttgttgcgc gtcggaaaca atatcgctga tcagcagctc gccttttgtc gtgggcttat
    16261 ctgttgccac cagcacctgg ggggccgctt tgtcggtagt tgtctcgctt gctaatgctt
    16321 cgctcaacaa cgcgagaaaa tcttgtgcag catcgctggc tttgccgcca ggcaatgtgg
    16381 tggtgtcaac gtcggcggta atcaagggcg ctaagcgaat cattcaggtt tcctcatggc
    16441 ggcgcgctgg gcgaactcat ccatcttttt ctgatcgagg cggttttctg caagcagtgc
    16501 cgccgtggat tgccgttcct gcagtgtctg ccaggcctgc aaacgttgtt tcttttctcg
    16561 ccagctgttc agggcaatgt caactttctg cgtccactga ttaagttgct ggcgatgctg
    16621 agtaatggct ttttccagcg tctggataaa ctgctgatag ttgatccagc ggttgctggt
    16681 catcccggca ctcatatcgc tgttgaggtt attgcgatat tcattctgat aatcgatcag
    16741 cattttgagc tgttcttccg cctgctgaca cccgcgacgc atttcaccca gcaggcgcgc
    16801 ggcatcctct acctcttttt ctgccagatc tttcagggtc gccagcgcac catgttctgc
    16861 catctaccgt tatctcctgg gttatgacac tgtcgggaaa atacgctcca gcccctggag
    16921 agacgcttcc cagtccgcac gttcaaaaat gccttgttgt aaatagccct ccagctgcgg
    16981 ccacagggcg atggctttat cgagcatcgg atcgctgcct ttggcatacg cgccgacgct
    17041 aaccagatcg cggttacgct gaaaactcga caacagctgt ttgaaggtgc gcactcgcgc
    17101 gtaatgttgc tcactgatca acgccgtcat tgcgcggctg atcgacgctt caatatcgat
    17161 agccggatag tgcccggctt ccgccagtcg gcgagacagc acaatgtgac cgtcgaggat
    17221 cgcccgtgcg gagtctgcaa tggggtcctg ctggtcatcg ccttcagtaa gcacggtata
    17281 aaacgcggta atcgagccgc cgccgctaat gccatttccg gcacgctcga ccagtgccgg
    17341 taatttggca aacaccgacg gtggataacc tttggtggcg ggtggttcgc caatcgccag
    17401 cgcaatttca cgctgggcca tcgcgtagcg ggtgagggag tccataatca gcaacacatg
    17461 ctgaccacga tcgcgaaaat cttcggcaat gcgcgtggca taggcggcac cttgcattcg
    17521 cagaagcgga gagacatccg ccggagcggc aatcaccacg gagcgtgcac gcccttcagc
    17581 accgaggata ttctcaataa aatcttttac ttcgcgccca cgttcaccaa tcaaacccac
    17641 gacaatgaca tcggcgcggg tgtaacgtgc catcatcccc agcagcacac ttttaccaac
    17701 gccggacccg gcaaacagcc ccatacgctg cccacgccca acggtaagca gggcattgat
    17761 tgggcgcacg ccggtgtcca gcacatgttc aatcggtgta cgttgcaacg ggttaaatgg
    17821 cggggtaatc agcgcaccgg tttccgtcgt atcgggggag ggcaggccat cgagcggctt
    17881 accgctgccg tccagaacgc gacctaataa cgccggaccg agcggcaact gcttgccgct
    17941 ttgcagtcct tcagccgaaa tgtttttggc ataaacccgc gcgccgggca ggacaccttc
    18001 cacttcctcc agcggcatta aaaacagccg ttgaccgtta aagccaacga cttcgctttc
    18061 tacttcgtgc gtttcgctgc cgttctggcg ttcaatgaca caggttgcgc cgagcggcaa
    18121 ttgtaatccg gtggcttcca gcaccagccc ggtagcgcgg gttaatcgcc catagcgacg
    18181 taccgcaggc aactgcgcca ttttggcttc aaagttatcc agcgtggtta gccagcgagt
    18241 caggcgcgtg gtcattacac cactcctggt gctgccagac ggcagagttc ttgccagcga
    18301 gtggcgacac tggcgtcgag atcgccttca tcggcggaga ctttacagcc gccaggatgg
    18361 agtgtgggat cgccccgcaa gcgccagcca tgcaaactta aggttgcgcc gagcatatca
    18421 tccacacgtt gcagatcatc cgggtgcacg cgcagctgtg gtttaccgct gaataacggt
    18481 tcttgctgca acaactgttg gatctgtttg atcagtgccg agttatccac tgttggcgtc
    18541 tgaccgatca cctgacgtgc cgcctccagc gccatctgca tcaggcgcga agcaatcaca
    18601 ctatcaagcg catcaagggt agtttgaaat tcgctgacca gttgctgcat ccgggcatga
    18661 attggcgctt gttgagactt cgcctctgcc agaccttgct ccagcccctg ggccagtcct
    18721 tcctgatagc cctgctcatg accttgctgg cgaccttcgg caatccccgc ctgataacct
    18781 tgctcatggg cctgcatttg cagttgcgcc agttgctgct caaggctagg ttcggcctct
    18841 tcaatgatgg tttcttccgg ctcgaccatg ggcacaaact ctgcctgtgg tggcgcgaga
    18901 tcgtccggcg tccaggtttt ccacggcaga ttatcagaca taggtatcct cgccgctgcc
    18961 aattaccatc tcgccagttt cggcaaggcg gcgcacaatc agcagaatcg ctttctgttc
    19021 gttttccacc tgcgacagac gcaccggacc acggttggcg agatcgtcgc gcagaatatc
    19081 ggcggcacgc tgcgacatat tgcgcaggaa tttctcgcgc agtggctgct cggccccttt
    19141 cagcgcgatc aacagcgatt cggaatccac ttcctgcaac agacgctgaa tgctgcgatc
    19201 gtcgacatcc accagatttt cgaacaggaa catctcgtcg ataatttttt gcgccagctc
    19261 gccgtcgaat tcgcgcacgg cggtaataac ggcttcttcc tgctgagttt tcatcaggtt
    19321 gataatttcg gctgccgttc tcacgccgcc cattttgctg cgcttgagat tctgaccgtc
    19381 gagcaagcca ttcagtactt cggtcagttc cgccagcgcg gctggctgca cgccgccaaa
    19441 ggtggcgata cgcaacatca cgtcgtggcg cagacgttca tcgaacaacg ccagaatatc
    19501 ggcggcctgc gcgcgcttca gatgcaccag aatggtggcg ataatttgcg gatgctcatc
    19561 gcgaatcaga tcggcggcac tctgcggctc cataaagttg agcgtttcaa taccgctggc
    19621 ggtatcgcga gtttcgagaa tatcttccag caggctggcg gcacgttcct cacccagagc
    19681 tttgaccaat accgaacgca gataatcgtt ggcgttgata ttcagtgcgg caaactgttc
    19741 agcttcttgc tcaaactccg ccagcacata ggttagctgc ttgttggaga tctgcgtgac
    19801 gttcgccatt gcagcgctca gggtttgcac ttcgcgctgg gagaggtgct tgaacacctc
    19861 tgccgcccgg tcttcgccaa tggtcatcag caggatgacg cttttatcgg tgcctgtcag
    19921 gttactcatg atcgttattt atccactggc gaatgaccag cgccaccacg cgcggatcgt
    19981 tatcagacat ttcacggata cgctggctca tgacttctgc ccccagacgt tggttagcgc
    20041 gtcgttgttg gagttgttcg tctttgctca ggcggacttc caccgcatct tccacttcct
    20101 cacgggcctg cgcctgttgc tgtacagctt tcatcgcctc ggcgcgacgt gttaactgcg
    20161 ggcgtaccgc tttccgccac agcagccacg ccaccagcag taccagcaac cagcgaccgg
    20221 cagccagcaa ctgatcgata aacgcttgct gttgccagaa cggcagttct ccgccgcttt
    20281 cgtcactgct attgaacggc gagttaacga cattgagcga gtcaccgcgt ttttcagaaa
    20341 agcccatcgc ctcgcgggtc agatcttcaa tttgcttcat ctgttcgttg ctgagaggca
    20401 atggtttacc gtctggcaag gttttgtaat tcaccactac cgcgactgac agacgttgca
    20461 catcgcccac gttcattttg gtatgacgaa tggtgcgatc gacttcgtag ttactggttt
    20521 cattccgctg tgtgctacgc ggcccgctat tgctggtggt gctcgcctgc tgctggcggt
    20581 tattttgatt tgccggaggc gtgctgattg gcgcgttatt cgcaggtgcc ggttgattcg
    20641 acaacgcccc cggtacgccg cccggataac cggaaccgct ttgctcgctc tcattaagct
    20701 ggcgtgaacg aagcgccgca tgagattcat caccgttagg gcgatactgt tcttccgttt
    20761 gttctttact ggcgaagtcc agttgcgccg tgacctgggc gtgaatatta ccgttaccaa
    20821 caataggcga caggattgct tcaatacgcc gctgaatacg gccttcgaca tcgctggcat
    20881 atttcaactg agcgtcatta agatcgcgcc cgctggtatt ggactgggtt aacagatgtc
    20941 cgccctgatc caccagcgtg acgtttcccg gcggcagacc agcaacggcg ctggaaacca
    21001 ggtgcacaat ggcgctaatt tgcccttcat cgagtgcgcg gccgggtaac agatttaccg
    21061 tcaccgatgc agaaggggat ttttgttcac ggacgaataa agacggtttc ggcattgcca
    21121 gatgtacgcg cgcccctttt accgggccga tagtttcgat ggtacgagaa agctcgcctt
    21181 ccagcgcccg ctgataattc acctgttcgc tgaactggct gataccaaac ttttcctgat
    21241 caagcagttc gaaaccgacc gcgccgcctt tgggcaaacc ttgttgtgcc aggcgcagac
    21301 gcagttcgtg aactttatct gccggaactt caatagcgcc gctggcttcg ctgaagcagt
    21361 aaggaatatt catttgcgtc agttggctga caattgcgcc accatcctga tcggaaagat
    21421 tgctgaataa tgtgcggtag tcgggggctt tcgcccacag gatcagtgcg accatgaccg
    21481 ccactgccgc ggaaccggca acaatcaatg gaattttcgg attcgcacgc aggcgattaa
    21541 gccactcaag agattttgtc tgggctgcag tcgcattcat cgcgcacctc gtggctgatt
    21601 atggacggcg ttattggcaa agtggaacaa gactcactcc cgggtcagca aactcgaaaa
    21661 attgacgctg ccattatttg tgctttcgtt tttttctatc cgccaataaa cccgtttttt
    21721 ttgttgctat ttagcgcctt tgtcttattg acttactggt aagctttgct accagaaatt
    21781 atccgggaga tgaaaatgtc agcgatacag gggattgaag gggttatcag ccagttacag
    21841 gctacggcga tgagtgcgcg tgcgcaggaa tcactgccac aaccgaccat tagttttgcc
    21901 gggcagctgc acgccgcgct cgatcgcata agcgatacac aaacagccgc ccgcacgcag
    21961 gcagaaaaat tcactctcgg tgaacccggc gtggcgttaa acgatgtgat gaccgatatg
    22021 caaaaagcct cagtttctat gcaaatgggg attcaggtgc gtaataagct ggtggcggct
    22081 tatcaggaag tgatgtccat gcaggtgtag gttttgtaac ctgttgttaa ttacatccga
    22141 tctcaccgcg tggggcatgg atggggcaaa ctcactcaat ttctggttta aaatggatac
    22201 ctgatcctga aagttttctg ccatccacga tccgtaaacg cgataacatg ttttgtcttc
    22261 gctttgctca taggaagagt gaactctttt gttagcctgg ggttccttct gatcattatt
    22321 gttcctgcct ttatctcttg ccacgccaga gccccctgga tacacataca ccaggatgaa
    22381 aacggtgagt tgtgttcaaa ctgtagtaca attctctcca gtatgaacag gaaagaatat
    22441 gctatgaacc cttatattta tcttggtggc gcaatacttg cagaggtcat tggtacaacc
    22501 ttaatgaagt tttcagaggg gtttacacgg ttatggccct ctgttggcac aattatttgc
    22561 tattgtgcat cattttggtt attagctcag acactggctt atatacctac agggattgct
    22621 tatgctatct ggtcaggagt cggtatcgtt ctgattagtt tgctttcctg gggatttttt
    22681 ggccaacgac tggacctgcc agccatcata ggcatgatgt tgatttgtgc cggtgtgtta
    22741 gtcattaact tgttgtcacg aagcgcaccg cattaaaaaa taatcatcct tgagataaca
    22801 ggaatctgat ttgtgcatat tatggctaat gttcggggca actttataat tatgattgca
    22861 tttgcgcgat cattgcgcct ttttgcataa tgagatcaat gcacatcaaa taaaataacg
    22921 ataggtcaca acaaagaggt ttttatgaaa aaacttatcg tttcaagtgt attggcattc
    22981 ataacatttt ctgcgcaggc cgcagcattt caggtcacta gtaatgaaat aaaaacagga
    23041 gagcaactta caacgtctca tgtattttct ggagttgggt gtgagggggg taatacatcg
    23101 ccctcattaa cctggtctgg tgctccagaa ggcaccaaaa gctttgccgt aactgtgtat
    23161 gatccagatg cgcctacagg cagcggatgg tggcactgga ctgttgctaa tattccagca
    23221 acggtaacat atttgcccgc tgatgcagga agacgtgatg gtacaaaact tccgactggt
    23281 gctgtccaag gccgaaatga ttttggctat gcgggatttg gtggagcatg tcctcccaaa
    23341 ggagataaac cccatcatta ccagtttaaa atatgggctc taaaaactga taagattcct
    23401 gtggattcta attccagcgg agcgttagtt ggttatatgc ttaatgccaa taaaattgca
    23461 accgctgaga taatacccgt ttatgaggta aagtaggata agagtatgct ggcaaaagat
    23521 aggactaacc taaagattga agaaatacgg atgcataaac atcatgagat tcacagggtt
    23581 aagcctctta tgccagcttc atgtcgtatc cgtcagggaa aaaaagttat caattgggag
    23641 acgcatagtc taactgttga taataatcag ataatattat ttccctgtgg atatgaatct
    23701 tatattgcga attacccaga agcaggtc
//
LOCUS       DAZPBM010000050        23515 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPBM010000050 DAZPBM010000000
VERSION     DAZPBM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.050"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..2064
                     /locus_tag="ACHUZQ_001847"
     CDS             <1..2064
                     /locus_tag="ACHUZQ_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1025556.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="ACHUZQ_001848"
     CDS             2080..3099
                     /locus_tag="ACHUZQ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR1025557.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHUZQ_001849"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHUZQ_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1025558.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHUZQ_001850"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHUZQ_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR1025559.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHUZQ_001851"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHUZQ_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1025560.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="ACHUZQ_001852"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="ACHUZQ_001852"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1025561.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="ACHUZQ_001853"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="ACHUZQ_001853"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1025562.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7646
                     /locus_tag="ACHUZQ_001854"
     CDS             6828..7646
                     /locus_tag="ACHUZQ_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR1025563.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7698..8444
                     /locus_tag="ACHUZQ_001855"
     CDS             7698..8444
                     /locus_tag="ACHUZQ_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1025564.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8418..9383)
                     /locus_tag="ACHUZQ_001856"
     CDS             complement(8418..9383)
                     /locus_tag="ACHUZQ_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1025565.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9380..10384)
                     /locus_tag="ACHUZQ_001857"
     CDS             complement(9380..10384)
                     /locus_tag="ACHUZQ_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR1025566.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10381..11658)
                     /locus_tag="ACHUZQ_001858"
     CDS             complement(10381..11658)
                     /locus_tag="ACHUZQ_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR1025567.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHUZQ_001859"
     CDS             11915..12967
                     /gene="fbaB"
                     /locus_tag="ACHUZQ_001859"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1025568.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13286..14140
                     /gene="gatY"
                     /locus_tag="ACHUZQ_001860"
     CDS             13286..14140
                     /gene="gatY"
                     /locus_tag="ACHUZQ_001860"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1025569.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHUZQ_001861"
                     /pseudo
     CDS             14169..15426
                     /gene="gatZ"
                     /locus_tag="ACHUZQ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15436..15888
                     /gene="gatA"
                     /locus_tag="ACHUZQ_001862"
     CDS             15436..15888
                     /gene="gatA"
                     /locus_tag="ACHUZQ_001862"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1025570.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15919..16203
                     /gene="gatB"
                     /locus_tag="ACHUZQ_001863"
     CDS             15919..16203
                     /gene="gatB"
                     /locus_tag="ACHUZQ_001863"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1025571.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16207..17562
                     /locus_tag="ACHUZQ_001864"
     CDS             16207..17562
                     /locus_tag="ACHUZQ_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1025572.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17610..18650
                     /gene="gatD"
                     /locus_tag="ACHUZQ_001865"
     CDS             17610..18650
                     /gene="gatD"
                     /locus_tag="ACHUZQ_001865"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1025573.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18756..19529
                     /locus_tag="ACHUZQ_001866"
     CDS             18756..19529
                     /locus_tag="ACHUZQ_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1025574.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHUZQ_001867"
     CDS             complement(19611..20510)
                     /gene="yegS"
                     /locus_tag="ACHUZQ_001867"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1025575.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            20916..21233
                     /gene="yegR"
                     /locus_tag="ACHUZQ_001868"
     CDS             20916..21233
                     /gene="yegR"
                     /locus_tag="ACHUZQ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR1025576.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHUZQ_001869"
     CDS             complement(21563..22924)
                     /gene="yegQ"
                     /locus_tag="ACHUZQ_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1025577.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23071..23403)
                     /locus_tag="ACHUZQ_001870"
     CDS             complement(23071..23403)
                     /locus_tag="ACHUZQ_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1025578.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         6062 a         5510 c         6025 g         5918 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttccaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 acaccattca cggaatagat gtttcgcgct ggcaggagcg gatcgactgg cagcgtgtgg
     7081 caaaaatgcg cgataacggt attcgcttac aatttgcctt tattaaggcg acggaaggcg
     7141 aaaagctggt ggacccctat ttttcgcgta actggcaact aagccgcgaa aatggcttgc
     7201 tgcgcggggc atatcattat ttctccccgt cggtagcagc accggttcag gtgcgattat
     7261 ttctgcaaac ggtagatttc tcacaaggcg atttccctgc ggtactggac gtagaagagc
     7321 gaggaaaatt atcggcaaaa gaattacgca agagggtaag tcagtggcta aaaatggtcg
     7381 aaaaaagtac ggggaaaaag ccgattattt actcaggagc cgttttttat cacacgaatc
     7441 tggcgggcta tttcaatgaa tatccgtggt gggtggctca ctactatcaa cgtcgtccgg
     7501 acaatgacgg catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca
     7561 tcaatggtcc ggtggatttt aatgtgttta atggcacggt ggaggagttg cagggattcg
     7621 ttgatgggat taaagaaacg ccttaaataa gaagcagtga tatactccca ccagactccc
     7681 tggcaggaaa aagacaaatg gaacaagcgc atacccagct gattgcccaa ctgaacgaac
     7741 gtattttagc ggcggataac acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg
     7801 ccgtgcgcag cggggtgctg gagcatggca atattttgcc cggtgagcgt gatttaagtc
     7861 agttaaccgg cgtttcgcgc attacggtgc gcaaggcgat gcaggcgctg gaagaagagg
     7921 gcgtggtgac gcgttcgcgc ggttacggca cgcagatcaa caacatcttc gaatactcgt
     7981 tgaaagaagc gcgaggtttt tctcagcagg tggtgttgcg cgggaaaaag cccgatacgc
     8041 tatgggttaa caagcgtgtc gtgaaatgtc ccgaagaagt cgcgcagcag ctggcggtcg
     8101 aagcaggaag tgatgtcttt ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat
     8161 cgattgagga atcgtgggtt ccggcgcatt taattcatga tgttgatgcc atcgggattt
     8221 cactttatga ctatttccgc agccagcaca tttacccaca acgtacgcgt tcccgcgtta
     8281 gcgcccggat gccggatgcc gagtttcagt cacatattca gttagatagc aaaataccgg
     8341 tgctggtgat caagcaagtg gcgcttgacc aacagcaacg gcctattgag tacagcatta
     8401 gccactgtcg cagcgatcta tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc
     8461 gcacaatcac cgccccgatg cccgacaacc cacgacgcca ctgcattgcc cagtaatacg
     8521 gcatccgcca gtggcagacc agaggccagc ccggcaagta cgccacctgc atgactgtcg
     8581 cccgccccaa tggtgtctac aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg
     8641 ttgctgaaat accatgcgcc ttctttatcg agacgaacga tcaacggcgc ggcaaatttc
     8701 tcctgccatt gctcgccaag tgttgttatc tctgcggata aagcaaaacg ttcggcggca
     8761 atctcagcct cttgacgatt gagcgacact aaaggtcgac aggccatgat ccgcgccagt
     8821 aatgcatccg ggatatcgcc aatacgtggg ccaaaatcga taaacggcgt cacgtcttgc
     8881 agctcttcca gccattccac taacaattcg ccgcagggcg aggccagttg ataaccggaa
     8941 aaatagagca ggctgccagg cgcaacggtt aatcgcgcca gccactggcg attccactga
     9001 ttttccacac cgctgaatga cataaaagtg cgttcgccat ccggctcaac cagcgccaga
     9061 caccagccgt tatcaccttc ggcgttatcg atcaggctga ttaagccctc ttttgccatc
     9121 cggttgcgaa taatctccgc ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg
     9181 atgccgaggc gctttaacgc cacggcaata ttcagtgcgc agccgccaac gttaacgctc
     9241 tgctgtttca gttcgatatc gcacccacgc caggggaggg cataagcgtc ggcgatcacg
     9301 tcaatgaccg ccgcgccgac gaccatcacc ggctgacgcg tggttaattc aggcagcagc
     9361 gtgtgtaatc gagcgccgct cataccgcct cccgttgttg acgatatttc gccagcgctg
     9421 tggcatagcg gttgaaatca agctgattta ccgcatccag ttccgccttt aacgcaggat
     9481 cgatagcgtt aacgccatgc aacgcgccgc aaattgccgt cgccatagca ccgatggtgt
     9541 ctgtgtcgcc gccaaggtta gcgcacagga cggcgcagcg attcgggtcg gtttgtgcta
     9601 gttcaaccag cgcaatggcg cacggaacgg actcaatagt gctggtacct gcgccaacga
     9661 cctggtaaag ctgttcgctg gcggattcgg tgccgtcggc attgcgcaca attttcagcg
     9721 caatctccag ccgcgccgcc agtgaggcgc tgaacgtagt gatgcgtttt tgctgtgcat
     9781 gtcgcgcaat tgaaggcagg gaatcaacaa tcgctgacca gctttctccg tcaatggcac
     9841 gagaaatcgc ccatgcgatg actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg
     9901 ggctggacgc cagcgctaca tcatcaataa aggaatcaac atcacgcgcc ggaagcaaac
     9961 aacctaatgg cgagacgcgc atcgctgcgc cgttggtcac gccgttgttt tccagttcag
    10021 caacgggttt accgtcgcga atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt
    10081 ttttgttaaa ggcgtcgaaa cgcagcgccc agtcgagaat attacgccca atcagatccg
    10141 gatcgatctt gccttcacgt tccagtaacg catccgccag acacagcgcc atcgaggtat
    10201 cgtcggtgaa ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggaa
    10261 gaaaacggtc aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg
    10321 gcatccccat cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt
    10381 tcatttaact tccccttgtg tcaacgcaat atcgcgatca tcgaccttga tagccgtaat
    10441 ttcgttgtcg gattcgcgga aaaaaatcat gaacagcacg gcgataatgg caatcatcac
    10501 cgcgccgaaa gtccacatcc cggaccagtt gaaagtcagt ccgtttaccg gttcctgata
    10561 agcgaacatc ttttccatca tcacaccgcc aagacgatag ccgagcaaac tgccgaagcc
    10621 ctggcagcag agcgtgatca gcccctgcgc agcggtacgc atatgcacgg gggctttttt
    10681 atcgacatag atgtaagcgg taacgtagta aaaatcgtaa cttacgccgt gaagcaaaat
    10741 accgaggaac agtaacgcgt aggtgaaata ttcatccgta ctaccgtaaa taaagaagcc
    10801 atagcggatc gcagcggtga ccagaccaag caataatacc tttttgatac caaagcgttt
    10861 agtgaaaaac ggcaatgcca gcataaagaa gatttcagag aactggccga gcgtcatcca
    10921 gccggtggcg tttttcatgc caacttcggt cagataaccg ttggcaaaga tgtaatagaa
    10981 cgccagtggc atcgcaaaca ggaatgaaca gaagaaaaag acgaggaagt ttttatcgcg
    11041 cagcaggatc agcgcatcca ggccgagcat gactttaata tccattttgc cggtgctttt
    11101 tggtggcgtg tcgggcagga aaaacgcaaa cacaccgagc agagcagaac ttccggcggt
    11161 aatcagcagc gggatgttag tcggtgagat atcggcatac cccagtattt gcggcaagaa
    11221 accacatgcc agaccggagg caatccagcc gatagtgccc atcacacgaa tgcgcgggaa
    11281 atcacgctcc acatccggca cgttggcaaa agcgatgctg ttagtcagcg caatggtcgg
    11341 catataggtt agagagtaag ccagcagtaa cggaaagaac ccggcaaaag tggtctgttg
    11401 cgcagcgaaa tacatcagca gcgcgcctgc gaacatcaat accgccagca ctttttgcgc
    11461 cgagaaaaag cggtcagtga tggagccaac cagaattggc gacaggatcg ccgcaatggc
    11521 ggtacaggca tacgaccagc caatttctcc ggcactaaaa ccgcttttac ttaaccacaa
    11581 ccacaatggc acaaaccacg cgccccagat aaaccattca acaaacatca tgaacgacag
    11641 ctttgctgtt gttttcatct tttaattcct tcatgacagg taatgtaagg tacgcgatga
    11701 caataccact taacaatacc ttttaaatac ctttgcggtg aaaacttgac cgcgctaaca
    11761 ttttttctga tgaatcgagc caacagaaaa cgctgaaaaa acatccaaaa gatggaaaaa
    11821 ctcgcccgta acgaccagga ttatgattgc ccgcaaaagg cgggccagga caatccatag
    11881 ccgatatcca atcggaattt acgggagcat agtaatgaca gatattgcgc agttgcttgg
    11941 caaagacgcc gacaaccttt tacagcaccg ttgtatgaca attccttctg accagcttta
    12001 tctccccgga catgactacg tagaccgcgt aatgattgac aataatcgcc cgccagcggt
    12061 gttacgtaat atgcagacgt tgtacaacac cgggcgtctg gctggcacag gatatctttc
    12121 tattctgccg gttgaccagg gcgttgagca ctctgccgga gcttcatttg ctgctaaccc
    12181 gctctacttt gacccgaaaa acattgttga actggcgatc gaagcgggct gtaactgtgt
    12241 ggcgtcaact tacggcgtgt tggcgtcggt atcgcggcgc tatgcgcatc gcattccatt
    12301 tctcgtcaaa cttaatcaca acgagacgct aagttacccg aatacctacg atcaaacgct
    12361 gtatgccagc gtggagcagg ccttcaacat gggcgcggtg gcggttggta cgactatcta
    12421 ttttggctcg gaagagtcac gtcgccagat tgaagaaatt tctgcggcct ttgaacgcgc
    12481 gcacgagctg ggcatggtga ccgtgctgtg ggcctatttg cgaaactccg cctttaagaa
    12541 agatggcgtt gattaccatg tttccgccga cctgaccggt caggcaaacc atctggcggc
    12601 aaccatcggt gcagatatcg tcaaacaaaa aatggcggaa aataacggcg gctataaagc
    12661 aattaattac ggttacaccg acgatcgtgt ttatagcaaa ttgaccagcg aaaacccgat
    12721 tgatctggtg cgttatcagt tagctaactg ctatatgggc cgggctgggt tgataaactc
    12781 cggcggtgct gcgggcggtg aaactgacct cagtgatgca gtgcgtactg cggttatcaa
    12841 caaacgcgct ggcggaatgg ggctgattct tggacgtaaa gcgttcaaga aatcgatggc
    12901 tgacggcgtg aaactgatta acgccgtgca ggacgtttat ctcgatagca aaattactat
    12961 tgcctgatac gcctcatacg aacagcacca ccccgtagga cggataaggc gtttacactg
    13021 catccggcaa cctatgcctg atgcgacgcg gaagcgtctt atcatgccta catagcactg
    13081 ccacgtatgt cggaaaaggc gtttacaccg catccggcat aaaaacacgc gcactttgct
    13141 acggcttccc tatcgggagg ccgttttttt gcctttcact cctcgaacag ttttcatatt
    13201 gtcgttttgt gatcgttatc tcgacattta aaaacaaatc atttcattat attttgaaat
    13261 cgaaaacaag cgacaggata tgaaaatgta cgtggtatcg acaaagcaga tgctgaacaa
    13321 cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg aaacgatgca
    13381 agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg gaacgcctgg
    13441 cacattcact tatgctggta cagaaaatct gttggcgctg gtcagcgcga tggcgaagca
    13501 atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg atatcgctca
    13561 gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc cttttgcgca
    13621 aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg tcagcgtcga
    13681 agcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg aagccgatgt
    13741 tttttatacc aaccccgctc aggcgcgtga atttgccgag gcaaccggaa ttgattccct
    13801 ggcggtcgcc atcggcacgg ctcatgggat gtatgccagc gcaccggcgc ttgatttttc
    13861 tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg gcgcgtcagg
    13921 gttatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa tcaacgttgc
    13981 aacggagctg aaaaatgcct tctcgcaggc gttaaaaaat tacctgaccg agcaccctga
    14041 agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg atgtggtgag
    14101 caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc atttaacaag
    14161 gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc ggcatatgtt
    14221 cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat cgcaacagca
    14281 cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc ggttataccg
    14341 gaatgacacc ggcagacttt cgcgaatttg tttttacgat tgccgataaa gtcggatttg
    14401 cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg cagcaagaaa
    14461 atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt cgtgctggct
    14521 tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata ccgttagcac
    14581 cagaaacggt tgcggaacga gctgctttgc tttgctgcgg aaagtgtggc gacagattgc
    14641 cagcgtgagc aactgagcta tgtcattggc actgaagttc cggttccggg cggtgaggcc
    14701 agcgccattc agtcagtaca catcacccat gttgaagatg ccgccaatac tttacgtacg
    14761 catcaaaagg cctttattgc ccgtgggctg acagaggcgt taacacgcgt gattgccatc
    14821 gtggtgcagc cgggcgtgga atttgatcac agcaatatta tccattatca gccgcaggaa
    14881 gcgcagccgc tggcgcaatg gatagaaaac acccgaatgg tttatgaagc acattctacc
    14941 gattaccaga cccggacggc ttattgggaa ttagtccgcg atcactttgc aatattgaaa
    15001 gtcggtcccg cattaacctt tgctttacgc gaggcgatat ttgcactggc acaaattgag
    15061 caggaactta tcgcccctga aaatcgcagc ggttgcctgg cggtaattga agaagtgatg
    15121 ctcgacgaac cgcaatactg gaaaaaatat tatcgtacgg gttttaacga ttcattactg
    15181 gatattcgtt acagcctgtc ggatcgtatt cgttattact ggccgcatag tcggattaaa
    15241 aatagcgtcg aaacgatgat ggtgaatctt gaaggcgtgg acatcccact gggcatgatt
    15301 agtcagtatc ttcccaaaca atttgaacgc attcagtccg gggaattatc agcaataccg
    15361 catcagctga ttatggataa aatttatgat gttttgcgcg cctatcgcta cggctgtgcg
    15421 gaataaggac ggtatatgac taacctgttt gttcgtagcg gaatttcttt tgtcgatcgt
    15481 agcgaagttt taacccatat cggtaatgag atgctcgcca aaggtgtggt tcatgatacc
    15541 tggccacagg cattaattgc cagagaagca gaattcccta ccgggataat gcttgagcag
    15601 cacgccattg caataccgca ttgtgaggcg attcatgcta agtcgtcagc catttatctg
    15661 ttaaggccaa caaataaagt tcattttcag caagcggatg atgataacga cgtggaggta
    15721 tcgttggtta ttgcgttgat tgtggagaat ccgcagcagc aattgaaact tttacgctgt
    15781 ttatttggca agttacaaca gcccgatatc gtcgagacac taatcactct tcctgaaacc
    15841 cagttaaagg aatacttcac aaagtatgtt ttagattcag acgaataaat ccctctgtaa
    15901 caataaaaag gactatttat gaaacgcaag attattgtcg cttgcggagg cgcggttgcg
    15961 acctctacga tggcggcgga agaaattaaa gagttgtgtc agaatcatag tattcctgtt
    16021 gaattaatcc agtgtcgggt taatgaaata gaaacctata tggatggtgt gcatttgata
    16081 tgcaccactg ccaaagtgga tcgtagtttt ggcgatattc cgttagttca cggcatgcct
    16141 tttatttctg gtgtcggtat cgaagcatta caaaataaaa ttctgactat cttacagggg
    16201 tgacctatgt tttcagaagt catgcgttat attctcgacc tcggccctac ggtgatgctt
    16261 ccgattgtca tcattatttt ttctaaaata ttaggcatga aggcaggcga ttgctttaaa
    16321 gcgggtctgc atatcgggat tggctttgtt ggcattggcc ttgtgattgg cttaatgctg
    16381 gattccattg gtccggcggc gaaagcgatg gcggaaaatt tcgacctgaa tctgcatgtg
    16441 gtcgatgtcg gctggccggg ctcttcacca atgacctggg cgtcgcaaat tgcgctggtg
    16501 gcgattccga ttgcgattct ggttaacgtg gcgatgttac tgacccgtat gacgcgggtg
    16561 gtaaatgttg atatctggaa tatctggcat atgaccttca ccggcgcgtt gctgcatctg
    16621 gcaaccggtt catggatgat agggatggca ggtgtggtaa ttcacgcggc gtttgtttat
    16681 aagctcggcg actggtttgc ccgcgatacc cgaaatttct ttgagctgga aggtattgct
    16741 attccgcacg gtacgtcggc gtatatgggg ccgattgcgg tgctggtcga tgctatcatc
    16801 gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg acgatattca gcgcaaattt
    16861 ggtccatttg gcgagcctgt caccgtgggt tttgtgatgg ggctgattat cggcatcctc
    16921 gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa aaacggcggc agttatgctg
    16981 ctgatgccac gggtgattaa acccatcatg gatggtttaa cgcccatcgc taagcaggcc
    17041 cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc tgattggcct ggatcccgca
    17101 ttactgctgg ggcatacggc ggtggtatcg gcaagcctga tttttatccc gctcaccatt
    17161 ttaattgctg tttgtgttcc gggtaatcag gtactgccgt ttggcgatct tgccaccatc
    17221 ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa atctgttccg caccttaatc
    17281 tcgggtgtca tcattatgag catcaccctg tggatcgcga cgcaaactat tggtttgcac
    17341 acccaactgg cggctaatgc tggggcgtta aaagccgggg gtatggtggc ttcaatggat
    17401 cagggcggtt ctcccattac ctggttactg attcaggttt tctccacgca aaatattccc
    17461 ggtttcatta ttatcggcgc aatttatctt accggtattt tcatgacctg gcgtagagcg
    17521 cgtggtttta ttaaacaaga gaaaaccgtt ctcgcagaat aatttttacc tgagggggag
    17581 gttatccccc ttaacaatca ggagttttta tgaaatcaat ggtgaatgat actgatggta
    17641 tcgtgcgcgt tgcagaaagc gtcattcctg aaattaaaca tcaggatgag gtgcgggtaa
    17701 aaattgccag ctcgggctta tgtggttccg atttacccag gatatttaaa aatggtgcac
    17761 attattatcc aataacgtta ggccatgaat ttagcggcta tattgatgcg gtgggatccg
    17821 gtgttgatga tttacaccct ggcgatgcgg ttgcctgtgt gccgttatta ccctgtttta
    17881 cttatccaga gtgtttgaaa gggttttatt cccagtgcgc aaaatatgat tttattggct
    17941 cgcggcgtga tggtggattt gctgaatata ttgtcgttaa gcgaaaaaat gtctttgctc
    18001 tacccacgga tatgcctatt gaggatgggg cttttattga gccgattacc gttggcctgc
    18061 atgcttttca tttagcgcaa ggttgtgaga ataaaaacgt tattattatt ggtgccggaa
    18121 ccattggcct gctggctatt cagtgcgctg tcgcgctggg agcaaagagt gtgacggcga
    18181 tcgacattag ctcagaaaaa ctggcattgg caaaatcttt cggtgcgatg caaacattta
    18241 acagtagcga aatgagcgcg ccgcaaatgc agagcgtttt acgcgaactg cgctttaatc
    18301 agcttatcct cgagacggct ggcgtaccgc aaactgtcga actggcggta gagattgccg
    18361 gtcctcatgc ccaactggcg ctggtgggca cgttgcatca ggatctgcat ttaacatcgg
    18421 caacgtttgg caaaatattg cgtaaagagc tgacggttat cggcagttgg atgaactatt
    18481 ccagcccttg gccggggcag gagtgggaaa cggcgagccg gttgctgaca gaacgtaagt
    18541 taagcctgga gccattaatc gctcaccgtg gaagctttga aagcttcacc caggcggtgc
    18601 gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct cattccatga aaccgcgggc
    18661 cagcgtgatg ctggcccggt attgtgcaaa acagatcatt caccaatggt cccccttcgt
    18721 ttacactagc cacaattgaa tatgggtaaa tgacgatgaa ttcattcgag cgaaggaata
    18781 agatcatcca attagtgaat gaacagggaa ccgtgcttgt tcaggatctg gcgggagtat
    18841 ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt tctcgaacaa aaaggcgtgg
    18901 ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg taatagtgaa accgagaccc
    18961 aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc gccaaaaaac agaatagcgc
    19021 aggcggcagt caaaatgatc catgaaggga tgaccgttat tctcgacagc ggaagtacga
    19081 caatgcttat cgccgaagga ttaatgaccg ctaaaaatat cacggtgatt accaacagtc
    19141 tcccggcagc gtttgccctt tccgaaaata aagacattac tctggtcgtc tgtggtggca
    19201 cggtccgcca taaaacgcgc tcgatgcatg gttccattgc cgagcgttca ttgcaggata
    19261 ttaatgccga tttaatgttt gtcggtgctg atggtattga tgcggttaat ggtattacga
    19321 cctttaatga aggttattcg attagcggtg cgatggtcac cgccgctaat aaagtgattg
    19381 ccgttctcga ttcatcgaaa tttaaccgtc gcggttttaa tcaggtactg ccaatcgaaa
    19441 aaattgacat tattattacc gatgatgctg tatcagaggt ggataaactg gcattgcaga
    19501 agacacgggt aaaattaatt acggtgtagt attgttctgt atgccggata aggtatttac
    19561 accgcatccg gcatttcgga ttctccctgg ttcaagagag aatagtttga ttaacgcaat
    19621 aatagacaat ctggtggtaa tcgacaacgc aacgccgccg gaagtatttc aatatgaaag
    19681 ttttgtccgc tcaacggttc gccatcaaga ttaaaagtga tttcgtgtgg ggcttgtata
    19741 tcaaaccacg acgaagcgcc ttcgataata ttcgggttat cttcgtcaga ttttaaggtt
    19801 gatacgagag ccggaagaat ttcatcgccg gtaaaaatgc gcagttgcag caagccatcg
    19861 ttaattaacg cgttcggaca caattgctga ccgccaccgg cctgacgccc gttaccaata
    19921 ccaatgacca gggcgtcacc ttgccagtga aagttttcac cgcggatttc acaacggtcc
    19981 ggttgcagag tatccatgcg cattaagcca tgaatgatgt aagagacgcc acccagcgcg
    20041 gcttttaatt tttccggcgt ttctgtggta atacgcgtcc caaatccgcc tgtcgccata
    20101 ttaataaaac aggtttgttt gttgacctgc gccatatcta tcgcaatggc gttaccggca
    20161 attgccagtt tcagcgcctt atccagtgcc tcaggaatcc ctacactggt ggcaaaatca
    20221 ttggcggttc ctaatggcaa aattcccagc gcgggtatgc catccccctc acactgaatc
    20281 aacgccgtag aaacttcatt aatggtgcca tcgccaccac cggcaatcac cgttgcgacg
    20341 cccaacttcc gggcctcctc tacatatcgt gcggcatcgc ctttctccca ggtgacccgc
    20401 acatggatcg tcattccttc ctcacgcaac agcataattg cttcgcgcaa gggtagattg
    20461 tcagtacttt tgccattaag aatcagtaag ctggcgggaa attctgccat gatcgtatgt
    20521 gcctttatga ttggtctgta gatagtgtag agcagaaaac aaaaggtgga gtcagaacag
    20581 gatgaaagtc ggaggataaa aagtcagttc aagtatggag gattcagtgg ttaccaccag
    20641 gagcttgcaa tggggacagc gaatactaac ggctgccacc gataaatttc aaaaaagagc
    20701 atatacctaa tattcaacta aacagtggca tcttcaatat aatatattaa agcccccatg
    20761 gagttaccct gaagggcctc aatgtccgta attcctactt atgtaggaaa tgttgtacag
    20821 aacatttatt ataatcctat tcaattataa taatcatgcc attattatat ttaaacacta
    20881 gagagtgtcg ttggtattta atgggggaag gtgagatgaa aaagatagct gctatatcat
    20941 taattagtat ttttcttata tctggttgtg ctgtgcataa tgatgaaaca agtatcggta
    21001 aatttggtct tgcatataaa agtaatattc agcgtaaact cgataaccaa tactacaccg
    21061 aagccgaagc ttctttagct aggggcagaa tatctggtgc agaaaatata gtaaaaaatg
    21121 atgcagccca tttctgtgtt actcagggca aaaaaatgca gatagttgac ctgaagacag
    21181 aaggtgcagg attacatggc gtcgctcgtc tgacattcaa atgtggagag tgagaatatt
    21241 ttttggtaag catcaaacat gcgcgttctg gttgtgctta gccggaacct gtgcgagcac
    21301 gatgccgtta cgtgaaaggc atcgtgctat gaagggagat tctatcgatg tggtcaatgg
    21361 aagacggtga ccagggatag ggcttatgca taaaaaataa gcccgtgtaa gggagattac
    21421 acaggctaag gaggtggttc ctggtacagc tagcatttta tgggttatgt ttttcagcga
    21481 aacggatgat aaccttaata aatgcagctg tatgtgatcg gtttctaaga attttccatc
    21541 cgggaaaaat aatcgaaatt aatcacttac cgtgtgggtt acgcgtggtt tccccggaga
    21601 aattacgcat cagcagcgcg taatttagct caagatcctg cgggaccggg agccacacag
    21661 tataaccatc gcctggtgcg accggcatcg cttcaccttt ggcgttttcc atgtgctcaa
    21721 gggtaaagtt aatgttgcct tgcggcgtca tcagctcaag gctgtcgcca acggagaatt
    21781 tatttttcac cgctaccgct gcgaggtccc ccttgcgctc accggtaaac tcaccaacaa
    21841 actgctggcg gtcagaaact gaataaccgt attcgtagtt ctgataatcg tcgtgagtat
    21901 gacgacgcag gaaaccttcg gtatagccac gatgcgccag accttccaga gtttccagca
    21961 ggctggtatc gaacggtttg cccgcagcgg catcatcgat agctttacga taaacctgtg
    22021 cggtgcgtgc gcaatagtag aaagatttag tacggccttc gattttcagc gaatgcacgc
    22081 ccattttggt caggcgttct acatgggcga tggcgcgcag atctttcgag ttcatgatgt
    22141 aagtgccgtg ctcatcttca aacgcggtca tatactcgcc cggacgctgg gcttcttcga
    22201 tcataaacac tttgtcggtt ggcgcgccga tacctagcgt cggctcaaca ttttgcaccg
    22261 gaatcggctc gtacttgtgt acgatgttgc caacgtcatc ttctttccct tcctggacgt
    22321 tgtactccca gcggcaggcg ttggtgcagg taccctggtt cggatcgcgc ttgttgatat
    22381 agccagagag caggcagcga ccggagtagg ccatgcacag cgcaccgtga acgaagatct
    22441 caatctccat atccggcacc tgattgcgga tctcttcaat ctcttccagc gacagctcgc
    22501 gagagaggat cacgcgggtc aggcccattt gctgccagaa tttcaccgtc gcccagttta
    22561 cggcgttagc ctgcaccgag aggtggatcg gcatttcagg gaagtgctca cgcaccagca
    22621 taatcagccc tggatcggac ataatcagcg catccggccc catttccacc accggtttca
    22681 ggtcacggat aaaggttttc agcttggcgt tgtgcggtgc aatgttgacc acgacataaa
    22741 actttttccc cagcgcgtgg gcttcattga tgccgagctg aagattttcg tggttgaatt
    22801 cgttgttgcg cacacgcagg gagtaacgcg gctggcccgc ataaacagca tctgcgccat
    22861 aagcgaaagc gtaacgcata tttttcagcg ttcccgccgg ggaaaggagt tccggtttaa
    22921 acataattct ctcgttctga tgacaggtca gctccgcttc acctgatgaa gcggttaggg
    22981 gagtgccccc actttaaggg cgggcattgt agcgctgtgg ggggatctgg taaagcgccg
    23041 gacgccgaca acccacgccc ggcatcgtaa ttacgtattg tctttcaccg tctggcttgt
    23101 gccattggct ttaacggagg caattccggt ttcacgagat tgcgcggtgg cgtacatctg
    23161 gctggagccg ataatttgat gattggcggc tttcagattg aaatagaatt tgccgttact
    23221 tgccgttttt ttctcatagc gttcttcttg cgggctgttg ctacgcaccg acgcgatgcc
    23281 cttttccgca gaggcttttg atgtatacag ctcgctggtg aggatagtct caccattgcc
    23341 cgcttttagc acaaaccgga actgattatc actgctctta ctgagttcaa accaaccagc
    23401 cataatgact ccttgattga aattgcgtat cgaaaacagc atctaaaggt gctgtttacc
    23461 gattaaagta tgagcgattt tctgatgcct gccagggcgg agagataatg tcagt
//
LOCUS       DAZPBM010000051        23408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPBM010000051 DAZPBM010000000
VERSION     DAZPBM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.051"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUZQ_001871"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUZQ_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR1025579.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHUZQ_001872"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHUZQ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR1025580.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHUZQ_001873"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHUZQ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1025581.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHUZQ_001874"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHUZQ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1025582.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHUZQ_001875"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHUZQ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1025583.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHUZQ_001876"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHUZQ_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR1025584.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHUZQ_001877"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHUZQ_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1025585.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHUZQ_001878"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHUZQ_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1025586.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHUZQ_001879"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHUZQ_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHUZQ_001880"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHUZQ_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1025587.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHUZQ_001881"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHUZQ_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR1025588.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHUZQ_001882"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHUZQ_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1025589.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHUZQ_001883"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHUZQ_001883"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1025590.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHUZQ_001884"
     CDS             complement(14171..14743)
                     /locus_tag="ACHUZQ_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1025591.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHUZQ_001885"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHUZQ_001885"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1025592.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHUZQ_001886"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHUZQ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1025593.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHUZQ_001887"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHUZQ_001887"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1025594.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHUZQ_001888"
     CDS             17912..18760
                     /locus_tag="ACHUZQ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1025595.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHUZQ_001889"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHUZQ_001889"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1025596.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHUZQ_001890"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHUZQ_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1025597.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHUZQ_001891"
     CDS             complement(21672..21872)
                     /locus_tag="ACHUZQ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1025598.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHUZQ_001892"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHUZQ_001892"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1025599.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHUZQ_001893"
     CDS             complement(23031..23387)
                     /locus_tag="ACHUZQ_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1025600.1"
                     /translation="MVTLYGIKNCDTVKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5854 a         6225 c         6268 g         5061 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgatggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa cggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgattta
//
LOCUS       DAZPBM010000052        23237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPBM010000052 DAZPBM010000000
VERSION     DAZPBM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.052"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHUZQ_001894"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHUZQ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1025601.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="ACHUZQ_001895"
     CDS             509..1138
                     /locus_tag="ACHUZQ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR1025602.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="ACHUZQ_001896"
     CDS             1165..2826
                     /locus_tag="ACHUZQ_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR1025603.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="ACHUZQ_001897"
     CDS             3065..3124
                     /locus_tag="ACHUZQ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1025604.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHUZQ_001898"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHUZQ_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR1025605.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHUZQ_001899"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHUZQ_001899"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1025606.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHUZQ_001900"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHUZQ_001900"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1025607.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHUZQ_001901"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHUZQ_001901"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1025608.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="ACHUZQ_001902"
     CDS             9507..10127
                     /locus_tag="ACHUZQ_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1025609.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="ACHUZQ_001903"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="ACHUZQ_001903"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1025610.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHUZQ_001904"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHUZQ_001904"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1025611.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHUZQ_001905"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHUZQ_001905"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1025612.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="ACHUZQ_001906"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="ACHUZQ_001906"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1025613.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHUZQ_001907"
                     /gene_synonym="dan"
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHUZQ_001907"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR1025614.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHUZQ_001908"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHUZQ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR1025615.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHUZQ_001909"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHUZQ_001909"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1025616.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHUZQ_001910"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHUZQ_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1025617.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHUZQ_001911"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHUZQ_001911"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1025618.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHUZQ_001912"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHUZQ_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1025619.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHUZQ_001913"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHUZQ_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1025620.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHUZQ_001914"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHUZQ_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1025621.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5654 a         6331 c         5907 g         5345 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcacccagtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc cttgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAZPBM010000053        23004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPBM010000053 DAZPBM010000000
VERSION     DAZPBM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.053"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHUZQ_001915"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="ACHUZQ_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1025622.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHUZQ_001916"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="ACHUZQ_001916"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1025623.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHUZQ_001917"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="ACHUZQ_001917"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1025624.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHUZQ_001918"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="ACHUZQ_001918"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1025625.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHUZQ_001919"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="ACHUZQ_001919"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1025626.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="ACHUZQ_001920"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="ACHUZQ_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1025627.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11312
                     /gene="ftsK"
                     /locus_tag="ACHUZQ_001921"
     CDS             7245..11312
                     /gene="ftsK"
                     /locus_tag="ACHUZQ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1025628.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11467..12078
                     /gene="lolA"
                     /locus_tag="ACHUZQ_001922"
     CDS             11467..12078
                     /gene="lolA"
                     /locus_tag="ACHUZQ_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1025629.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12089..13432
                     /gene="rarA"
                     /locus_tag="ACHUZQ_001923"
                     /gene_synonym="mgsA"
     CDS             12089..13432
                     /gene="rarA"
                     /locus_tag="ACHUZQ_001923"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1025630.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13523..14815
                     /gene="serS"
                     /locus_tag="ACHUZQ_001924"
     CDS             13523..14815
                     /gene="serS"
                     /locus_tag="ACHUZQ_001924"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1025631.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15054..17498
                     /gene="dmsA"
                     /locus_tag="ACHUZQ_001925"
     CDS             15054..17498
                     /gene="dmsA"
                     /locus_tag="ACHUZQ_001925"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR1025632.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17509..18126
                     /gene="dmsB"
                     /locus_tag="ACHUZQ_001926"
     CDS             17509..18126
                     /gene="dmsB"
                     /locus_tag="ACHUZQ_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1025633.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18128..18991
                     /gene="dmsC"
                     /locus_tag="ACHUZQ_001927"
     CDS             18128..18991
                     /gene="dmsC"
                     /locus_tag="ACHUZQ_001927"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR1025634.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19027..19653)
                     /gene="ycaC"
                     /locus_tag="ACHUZQ_001928"
     CDS             complement(19027..19653)
                     /gene="ycaC"
                     /locus_tag="ACHUZQ_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1025635.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            19968..21116
                     /locus_tag="ACHUZQ_001929"
     CDS             19968..21116
                     /locus_tag="ACHUZQ_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1025636.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21326..22756
                     /locus_tag="ACHUZQ_001930"
     CDS             21326..22756
                     /locus_tag="ACHUZQ_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1025637.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22757..>23004)
                     /locus_tag="ACHUZQ_001931"
     CDS             complement(22757..>23004)
                     /locus_tag="ACHUZQ_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1025638.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         5651 a         5726 c         6085 g         5542 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cataaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc atttttgtgg tgaaaacaag catcgtggca
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataattc agataatccc
      841 gacggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgactg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcatgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca accttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattcaggc caaagcaccg
     1261 cgcgaggatc gggcgaccac cagaggatgg gggcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gccccccaga gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgctta aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcgtcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgac
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatctgacca tcctgcatta cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggcgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcatgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgtaat gttgctgccg gtaattgtgg attttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtagcg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcaacag tgaactttta cctgaaccgc tgcgaccaac
     3841 caacacggca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccacctcg ccacgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca cgccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaatgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgtcg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta acggtgcagt gcccagcaga atgagcgccg cagcccagtt
     4501 tgaggggaag attgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccgcc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgca tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt caggggcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccttg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc tgacgacgtc ctattttcgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc ggcacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagccacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccacagcagc
     8461 cgtattatgc acctgcagct gaacaacctg tgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgccc ctgcgccaga acaaccggtg gcaggtaacg
     8581 cctggcaagc cgaagagcag caatccactt ttgctccaca gtctacatac cagactgagc
     8641 aaacttatca gcaaccagcc gctcaggagc cgttgtacca acagccgcaa cccgttgaac
     8701 agcagcctgt tgtggagcct gaacccgttg tagaagagac aaaacccgcg cgtccgccac
     8761 tttactattt tgaagaagtg gaagagaagc gagcccgtga acgtgaacaa cttgcggcct
     8821 ggtatcaacc gattccagaa ccggttaaag aaccagaacc gatcaaatct tcgctgaaag
     8881 caccttctgt tgcagcagta cctccagtag aaaccgctgc cgctgtttct ccgctggcat
     8941 ctggcgtgaa aaaagcgaca ctggcgacgg gggctgccgc aaccgttgcc gcgccagtct
     9001 tcagtctggc aaatagcggt ggaccgcgtc cgcaggtcaa agaggggatt ggcccgcagt
     9061 tgccacgacc gaaacgtatc cgcgtgccaa ctcgccgtga attggcgtct tacggtatta
     9121 agctgccctc acagcgtgcg gcggaagaaa aagcccgtga agcccagcgc aatcagtacg
     9181 attctggcga tcagtacaac gatgatgaaa tcgatgcgat gcagcaggat gaactggcac
     9241 gtcagttcgc ccagacacag cagcaacgct atggcgaaca gtatcaacat gatgtgcccg
     9301 taaacgcaga agatgcagat gctgcggcag aggctgaact ggctcgtcag tttgcgcaaa
     9361 ctcaacaaca acgttattcc ggcgaacaac cggctggggc gaatccgttc tcgctggatg
     9421 attttgaatt ttcgccaatg aaagcgttgc tggatgatgg tccacacgaa ccgttgttta
     9481 cgccaattgt tgaacctgta cagcagccgc aacaaccggt tgcaccgcag cagcagtatc
     9541 agcagccaca acagccggtt gcgccacagc agcaatatca gcagccgcaa cagccggttg
     9601 cgccgcagcc acaatatcag cagccgcaac aaccggttgc gccacagcag caatatcagc
     9661 agccgcaaca accagttgcg ccgcagccgc agtatcaaca gccgcaacaa ccggttgcgc
     9721 cgcagcagca gtatcagcag ccacaacagc cagttgcgcc acaaccgcag gataccctgc
     9781 ttcatccgct gttgatgcgt aatggcgaca gccgtccgtt gcataaaccg acgacgccgc
     9841 tgccttctct ggatttgctg acaccgccgc cgagcgaagt ggagccggta gatacctttg
     9901 cgcttgaaca aatggctcgc ctggtggaag cgcgtctggc tgatttccgt attaaagccg
     9961 atgttgtcaa ttactctccg gggccggtta tcactcgctt tgaattgaac ctggcaccgg
    10021 gcgtaaaagc ggcgcgcatt tctaacttgt cacgggacct tgcccgttca ctttcgacgg
    10081 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccga
    10141 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    10201 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    10261 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aaatctgtcg
    10321 gtgtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    10381 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
    10441 cggaagtcgt tactgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    10501 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    10561 aaaaaattgc tgaagccgat cgcatgatgc gtccgattcc agacccgtac tggaagccgg
    10621 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accatacatt gtggtgttgg
    10681 ttgacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    10741 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaact cagcgtccat
    10801 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    10861 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggcgcggaa tcactgctgg
    10921 ggatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    10981 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    11041 cacagtatgt tgatggcatc acttccgaca gtgaaagcga aggtggcgcg ggcggtttcg
    11101 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    11161 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    11221 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    11281 gtgaagtgct ggctccaccg ccgtttgact aattaatgca ttgccggata aggcgcggta
    11341 gcgtcgcatc cggcactcta tcaactgaaa attcagtatt ttcttctttc ctcaagctga
    11401 ttattagcct ggaatagaga gtagagggaa ctcccgatcg ggagtgacgt aatttgagga
    11461 ataatgatga aaaaaattgc catcacctgt gcattactct caagcttagt agcaagcagc
    11521 gtttgggctg atgccgcaag cgatctgaaa agccgcctgg ataaagtcag cagcttccac
    11581 gccagcttca cacaaaaagt gactgacggt agcggcgcgg cggtgcagga aggtcagggc
    11641 gatctgtggg tgaaacgtcc aaacttattc aactggcata tgacacaacc tgatgaaagc
    11701 attctggttt ctgacggtaa aacactgtgg ttctataacc cgttcgttga gcaagctacg
    11761 gcaacttggc tgaaagatgc caccggtaat acgccgttta tgctgattgc ccgcaaccag
    11821 tccagcgact ggcagcagta caatatcaaa cagaatggcg atgactttgt cctgacgccg
    11881 aaagccagca atggcaatct gaagcagttc accattaacg tgggacgtga tggcacaatc
    11941 catcagttta gcgcggtgga gcaggacgat cagcgcagca gttatcaact gaaatcccag
    12001 caaaatgggg ctgtggatgc agcgaaattt accttcaccc cgccgcaagg cgtcacggta
    12061 gatgatcaac gtaagtagag gcacctgagt gagcaatctg tcgctcgatt tttcggataa
    12121 tacttttcaa cctctggccg cgcgtatgcg gccagaaaat ttagcacagt atatcggcca
    12181 gcaacatttg ctggctgcgg ggaagccgtt gccgcgcgct atcgaagccg ggcatctgca
    12241 ttctatgatc ctctgggggc caccgggtac cggcaaaaca accctcgctg aagtgattgc
    12301 ccgctatgcg aacgctgatg tggaacgtat ttctgccgtc acctctggcg tgaaagagat
    12361 tcgcgaggcg atcgagcgcg cccggcaaaa ccgcaatgca ggtcgccgca ctattctttt
    12421 tgttgacgaa gtgcaccgat tcaacaaaag ccagcaggat gcatttctgc cacatattga
    12481 agacggcacc atcactttta ttggcgcaac cactgaaaac ccgtcgtttg agcttaattc
    12541 ggcactgctt tcccgtgccc gtgtctatct gttgaaatcc ctgggtacag aggatattga
    12601 gcaagtacta actcaggcga tggaagacaa gacccgtggt tatggtggtc aggatattgt
    12661 tctgccagat gaaacacgac gcgccattgc tgaactggtg aatggcgacg cgcgccgggc
    12721 gttaaatacg ctggaaatga tggcggatat ggccgaagtc aatgatagcg gtaagcgggt
    12781 cctgaagcct gaattattga ccgaaatcgc cggtgagcga agcgcccgct ttgataacaa
    12841 aggcgatcgc ttttacgatc tcatttccgc actgcataag tcggtacgtg gtagcgcacc
    12901 cgatgcggcg ctctactggt atgcgcgaat tattaccgct ggtggcgatc cgttatatgt
    12961 cgcgcgtcgt tgcctggcaa ttgcttccga agatgtcggc aatgccgacc cacgggcgat
    13021 gcaggtggca attgcggcct gggattgctt tactcgcgtt ggcccggcgg aaggtgaacg
    13081 cgccattgct caggcgattg tttacctggc ctgcgcgcca aaaagcaacg ctgtctacac
    13141 tgcgtttaaa gctgcgctgg cggatgctcg cgaacgcccg gattatgacg tgccggttca
    13201 tttgcgtaat gcgccgacga aattaatgaa ggaaatgggc tacgggcagg aatatcgtta
    13261 cgctcatgat gaagcaaacg cttatgctgc cggtgaggtt tacttcccgc cggaaatagc
    13321 acaaacacgc tattatttcc cgacaaacag gggccttgaa ggcaagattg gcgaaaagct
    13381 cgcctggctg gctgaacagg atcaaaatag ccccataaaa cgctaccgtt aatgttatcg
    13441 ttgcggtaat gttgttactg tatccctgtg gtcgcaggct gtggccacat ctcccattta
    13501 attcgataag cacaggataa gcatgctcga tcccaatctg ctgcgtaatg agccagacgc
    13561 agtcgctgaa aaactggcac gccggggctt taagctggat gtagataagc tgggcgctct
    13621 tgaagagcgt cgtaaagtat tgcaggtcaa aacggaaaac ctgcaagcgg agcgtaactc
    13681 ccgatcgaaa tccattggcc aggcgaaagc gcgcggggaa gatatcgagc ctttacgtct
    13741 ggaagtgaac aaactgggcg aagagctgga tgcagcaaaa gccgagctgg atgctttaca
    13801 ggctgaaatt cgcgatatcg cgctgaccat ccctaacctg cctgcagatg aagtgccggt
    13861 aggtaaagac gaaaatgaca acgttgaagt cagccgctgg ggtaccccgc gtgagtttga
    13921 ctttgaagtt cgtgaccacg tgacgctggg tgaaatgcac tctggcctcg actttgcagc
    13981 tgcagttaag ctgactggtt cccgctttgt ggtaatgaaa gggcagattg ctcgcatgca
    14041 ccgcgcactg tcgcagttta tgctggatct gcataccgaa cagcatggct acagtgagaa
    14101 ctatgttccg tacctggtta accaggacac gctgtacggt acgggtcagc tgccgaaatt
    14161 tgctggcgat ctgttccata ctcgtccgct ggaagaagaa gcagacacca gtaactatgc
    14221 gctgatccca acggcagaag ttccgctgac taacctggtg cgcggtgaaa tcatcgatga
    14281 agatgatctg ccaattaaga tgaccgccca caccccatgc ttccgttctg aagccggttc
    14341 atatggtcgt gacacccgtg gtctgatccg tatgcaccag ttcgacaaag ttgaaatggt
    14401 gcagatcgtg cgcccggaag actcaatggc ggcgctggaa gagatgactg gtcatgcaga
    14461 aaaagtcctg cagttgctgg gcctgccgta ccgtaaaatc atcctttgca ctggcgacat
    14521 gggctttggc gcttgcaaaa cttacgacct ggaagtatgg atcccggcac agaacaccta
    14581 ccgtgagatc tcttcctgct ccaacgtttg ggatttccag gcacgtcgta tgcaggcacg
    14641 ttgccgcagc aagtcggaca agaaaacccg tctggttcat accctgaacg gttctggtct
    14701 ggctgttggt cgtacgctgg ttgcagtaat ggaaaactat cagcaggctg atggtcgtat
    14761 tgaagtacca gaagttctgc gtccgtatat gaacggactg gaatatattg gctaataccc
    14821 aatttttctg aatctaaaaa gcgcctgcgg gcgctttttt tgtctccctt tgataccgaa
    14881 caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta cctatctctt
    14941 tgattttcaa attattcgat gtatacaagc ctatatagcg aactgctata gaaataatta
    15001 cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc attatgaaaa
    15061 cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg gtaaaaacga
    15121 cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt agtcggattg
    15181 cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc tggagcgcct
    15241 gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca
    15301 aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac caggttcgcg
    15361 cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc
    15421 cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc tgggaagaag
    15481 cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc aacgagtcta
    15541 tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc tggccgccgg
    15601 gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac cattacggcg
    15661 actactcctc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc tgggcagatg
    15721 gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt aataaccctg
    15781 gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca cgccagaaat
    15841 ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc gggcgcgaag
    15901 atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt ctggcgtacg
    15961 tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc gttggctacg
    16021 atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct tatattctgg
    16081 gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcacaaatc accggtgttc
    16141 cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg gcgtttatca
    16201 gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt gctatctcga
    16261 tgctggcgat tctgaccggt aacgttggta ttaatggagg gaacagcggc gcgcgtgaag
    16321 gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc cagaccagca
    16381 tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg
    16441 atggtgtacg cgggaaagat aagctggatg tgccgatcaa aatgatctgg aactatgccg
    16501 gtaactgcct ggttaaccag cattctgaaa tcaaccgtac ccatgaaatc cttcaggatg
    16561 ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg gcgaaatatg
    16621 ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgcg ctggatgcat
    16681 cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg cgctttgaat
    16741 gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt gagcaacagt
    16801 ttactgaagg ccgtacccag gaagagtgga tgcggcatct gtatgcccag tcgcgggaag
    16861 cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg
    16921 acccacaagg gcatcacgtt gcttataaag ccttccgtga agatccgcag gcaaacccac
    16981 tgactacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac attgccgcta
    17041 cctgggaatt gccagaaggc gatgtgatcg atccactgcc gatctacacg ccgggctttg
    17101 aaagttatca ggatccgctg aacaaacagt atccgctgca gcttacgggt ttccactata
    17161 aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcagct tgccgtcagg
    17221 aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc gataaagtca
    17281 ggatctttaa cgatcgtggt gaagttcata ttgaggcgaa agtgacgcca cgaatgatgc
    17341 caggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatgcaaaa cgtgtcgata
    17401 agggtggttg tattaacgta ctgaccactc aacgtccgtc tcctctcgct aaggggaatc
    17461 cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat gacaacccag
    17521 tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga gctggcttgt
    17581 aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga atatgctggc
    17641 ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta ctatctgtcg
    17701 atttcgtgca accactgcga agatccggct tgtactaaag tctgcccgag cggtgcgatg
    17761 cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg ctgccgctat
    17821 tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg ccatatgacc
    17881 aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct
    17941 tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa acatggcgat
    18001 ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat tgtgatcaaa
    18061 cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa cccgaaggag
    18121 gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg tcttcgggca
    18181 atgtgtggcg ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc
    18241 agaagcccag cagcgtgtta tcgcctgcat gtttggctta tgggtgctga tgggcattgg
    18301 ctttatcgct tctatgctcc atcttggttc accgatgcgc gcttttaact cgctcaaccg
    18361 ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt ttgccgtagg
    18421 cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc gtacgctttg
    18481 gctggtggtg acgatggttc ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa
    18541 cagcattgat accgttccga cctggtacag catctggacg ccgatgggct tcttcctgac
    18601 gatgttcatg ggcggcccgt tactggggta cctgttgctg agtctggctg gcgtcgatag
    18661 ctgggcgatg cgtctgctgc cagccatttc cgtactggca ctggtggtga gtggcgtggt
    18721 gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc aggccgcagc
    18781 gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct
    18841 gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc tactatccgt
    18901 ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct atggtttgca
    18961 tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt cggccccggt
    19021 tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac gataatcagg
    19081 aatatggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt cacggtgcag
    19141 ctcgcaagcc acgccgaacc atgtcatcaa ttgcgcacct gcttgcgaca tgcgatccca
    19201 tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacaa caaacacatc
    19261 aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg ttaccacacc
    19321 ggcaataatt aactgttttt tacctgtcgc tttgactgct tttacaaaat cttcgttatc
    19381 ccaggcgtta atattgcccg ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag
    19441 ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa tagttggtaa
    19501 gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact tatcgggttc
    19561 gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa cagcagcatc
    19621 atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag agtcagtggt
    19681 acgcctgaaa tcggtcagac gtgatgcatt gctctgaaag catagacggg aaatatgagt
    19741 ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat
    19801 aaaaacaatc agatgagatt aatcatctct tcagtattag cgttatgaat gagtaatata
    19861 aatcgataat accgccgttg cgatatgcgc gtattttcac ccattgacaa tgtttttggc
    19921 ggtggcatga tgcgcatgaa atttgaactt cctcacggtt ttaattcatg tccacgtata
    19981 cccggcctgt catgcttttg ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt
    20041 taaatacact cgtgccgctt tggctcgccc aggaacacat gtccacatgg caggtaggcg
    20101 ttgtcagctc atcctatttt accggcaacc ttgtcggtac attgctgaca gggtatgtca
    20161 ttaaacgcat tggttttaac cgcagctatt atctggcctc cttcattttt gccgctggct
    20221 gtgccggcct tggcctgatg attggattct ggagttggtt ggcgtggcgt tttgtcgcgg
    20281 gcgtcggctg tgccatgatt tgggtagtcg ttgagagcgc gctgatgtgc agtgggacgt
    20341 cacgtaaccg tgggcgtttg cttgctgcat acatgatggt ttattacgtg ggaacgtttt
    20401 taggccagtt actggtcagc aaggtctcaa ccgagctgat gagcgtattg ccgtgggtta
    20461 caggtttgac attggcaggg atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg
    20521 ctgaaaacca tgattcgacg tcaattacgg caatgctaaa actccgtcag gcgcggcttg
    20581 gcgtgaatgg ctgcattatc tcaggaatcg ttctgggatc tctatatggc ctgatgcctc
    20641 tgtacctcaa tcacaaaggg gtgagcaatg ccagtattgg tttctggatg gcggtactgg
    20701 tcagtgcggg tatcctcgga caatggccga ttggacgtct ggcggataag tttggtcgac
    20761 tgctggtttt gcgtgttcag gtctttgtcg tcattctcgg cagtattgcg atgcttagcc
    20821 aggcggcgat ggccccagcg ttattcatcc tcggtgccgc tggctttacg ctatatccgg
    20881 tggcgatggc atgggcttgc gagaaagttg aacatcatca actggtggcg atgaaccaag
    20941 ccttactgtt gagctatact gtgggaagtc tgcttggccc gtcatttacc gctatgctaa
    21001 tgcagaattt ctccgataat ttattgttta tcatgatcgc cagcgtatcg tttatctatt
    21061 tgctgatgct gctgcgcaac gccggtcata cgccgaaacc cgttgctcac gtgtaaatga
    21121 attcaagcag agtgtgaact tactgtttca cactctgctt ttttgtttct tctatctgac
    21181 ttgctttatt ccaaatttta ttcgtttaaa aataaaatgt gtagcaggtt ataattttgc
    21241 atttcgctat ttccgcactt cttatttgcc gcgcataatc cctcgtttta cagatgcccc
    21301 tataattttg gcgaaggatt tgtctatggc tgggaatgtt caggaaaaac agctgcgatg
    21361 gtacaacatt gcgctgatgt cttttatcac tgtctggggt tttggcaacg ttgttaataa
    21421 ctacgccaac caggggctgg tggttgtttt ttcatgggtg tttatctttg cgctctattt
    21481 cacaccttat gcgctaattg ttggtcagtt aggctcgacc ttcaaagatg ggaagggcgg
    21541 ggtcagtacc tggattaaac acacgatggg acccggactg gcttatctcg ccgcgtggac
    21601 ctactgggtg gtgcatattc cctatctggc acaaaaaccc caggcaattc tgattgcgct
    21661 cggttgggcg atgaaaggcg acggttcgct aatcaaagaa tattcagtcg tagcgttaca
    21721 ggggttaacg ctggtgctgt ttatcttctt tatgtgggtt gcttcacgcg gtatgaaatc
    21781 gctgaaaatc gtcggttctg tggcagggat tgctatgttt gttatgtcgc tcctgtatgt
    21841 ggcgatggcg gtaaccgcgc ctgcaattac tgaagtgcat attgcgacca caaacattac
    21901 ctgggaaacg ttcattcctc atatcgactt tacctacatt accactattt caatgctggt
    21961 tttcgcggtt ggcggagcag agaagatttc tccttacgtt aatcaaacgc gcaacccagg
    22021 aaaagaattt ccaaaaggga tgttatgcct ggcggtgatg gttgcggttt gtgccattct
    22081 gggctcgctg gcgatgggga tgatgtttga ttcgcgtaat atcccggatg acttaatgac
    22141 caacggtcag tattacgcct ttcagaagct gggcgagtat tacaacatgg gtaatacttt
    22201 aatggtgatt tacgccattg cgaataccct gggacaagta gcggcgctgg tattctcgat
    22261 tgatgccccg cttaaagtgc tattaggtga tgctgacagc aaatatattc cagccagttt
    22321 atgtcgtacc aacgcttctg gtacgcccgt taatggctat tttctgaccc tggtactggt
    22381 ggcgattctg attatgctgc cgactctcgg cattggtgat atgaacaatc tctataaatg
    22441 gctgttgaac cttaattcgg tagtgatgcc gctgcgttat ttatgggtat ttgttgcatt
    22501 tattgcagtc gttcgcctgg cgcagaaata taaaccagaa tatgtcttta ttcgtaataa
    22561 gcctctggca atgaccgtcg ggatttggtg ttttgccttt accgcctttg cctgtttgac
    22621 ggggatcttc ccgaaaatgg aagccttcac tgcagagtgg accttccagt tggcgctgaa
    22681 tgttgcaacg ccgtttgtgc tggtaggatt aggactgata ttcccgctgc tggcgcgtaa
    22741 agcgaatagt aaataattat tgtggtcggt gacgctgact ctggcagatc tgacccgttt
    22801 ttacataatc gagaaatgcg cgcaagccgc aggacatata ctggcggttt gggtaataga
    22861 tctggaagcc tggacgctct gtgatccagt cttccagtac acattctaaa cgaccggtat
    22921 caaggtactc cttgatctgt tcatagagca gatacccgat accagcaccc atcaaaacgg
    22981 catccagttc agcatcaaca tcat
//
LOCUS       DAZPBM010000054        22592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPBM010000054 DAZPBM010000000
VERSION     DAZPBM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.054"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHUZQ_001932"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHUZQ_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1025639.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUZQ_001933"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUZQ_001933"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1025640.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHUZQ_001934"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHUZQ_001934"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1025641.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUZQ_001935"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUZQ_001935"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1025642.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUZQ_001936"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUZQ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1025643.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="ACHUZQ_001937"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="ACHUZQ_001937"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR1025644.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHUZQ_001938"
     CDS             complement(8188..8697)
                     /gene="eco"
                     /locus_tag="ACHUZQ_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1025645.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(8811..8975)
                     /gene="yojO"
                     /locus_tag="ACHUZQ_001939"
     CDS             complement(8811..8975)
                     /gene="yojO"
                     /locus_tag="ACHUZQ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1025646.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="ACHUZQ_001940"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="ACHUZQ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1025647.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="ACHUZQ_001941"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="ACHUZQ_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1025648.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="ACHUZQ_001942"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="ACHUZQ_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1025649.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="ACHUZQ_001943"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="ACHUZQ_001943"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1025650.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="ACHUZQ_001944"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="ACHUZQ_001944"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1025651.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="ACHUZQ_001945"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="ACHUZQ_001945"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1025652.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="ACHUZQ_001946"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="ACHUZQ_001946"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1025653.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14937..15560
                     /gene="ccmA"
                     /locus_tag="ACHUZQ_001947"
     CDS             14937..15560
                     /gene="ccmA"
                     /locus_tag="ACHUZQ_001947"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1025654.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="ACHUZQ_001948"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="ACHUZQ_001948"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1025655.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="ACHUZQ_001949"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="ACHUZQ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1025656.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="ACHUZQ_001950"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="ACHUZQ_001950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1025657.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="ACHUZQ_001951"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="ACHUZQ_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1025658.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="ACHUZQ_001952"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="ACHUZQ_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1025659.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="ACHUZQ_001953"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="ACHUZQ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1025660.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="ACHUZQ_001954"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="ACHUZQ_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1025661.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="ACHUZQ_001955"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="ACHUZQ_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1025662.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..>22592
                     /locus_tag="ACHUZQ_001956"
     CDS             22225..>22592
                     /locus_tag="ACHUZQ_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1025663.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5055 a         5670 c         6428 g         5439 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtctg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcgtcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccatc gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat gg
//
LOCUS       DAZPBM010000055        22335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPBM010000055 DAZPBM010000000
VERSION     DAZPBM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.055"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(134..3283)
                     /gene="acrB"
                     /locus_tag="ACHUZQ_001957"
     CDS             complement(134..3283)
                     /gene="acrB"
                     /locus_tag="ACHUZQ_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1025664.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="ACHUZQ_001958"
     CDS             complement(3306..4499)
                     /gene="acrA"
                     /locus_tag="ACHUZQ_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1025665.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4641..5288
                     /gene="acrR"
                     /locus_tag="ACHUZQ_001959"
     CDS             4641..5288
                     /gene="acrR"
                     /locus_tag="ACHUZQ_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1025666.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5416..8778
                     /gene="mscK"
                     /locus_tag="ACHUZQ_001960"
     CDS             5416..8778
                     /gene="mscK"
                     /locus_tag="ACHUZQ_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1025667.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8839..8937
                     /locus_tag="ACHUZQ_001961"
                     /pseudo
     CDS             8839..8937
                     /locus_tag="ACHUZQ_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="ACHUZQ_001962"
     CDS             complement(8990..9151)
                     /gene="rsmS"
                     /locus_tag="ACHUZQ_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1025668.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9165..9692)
                     /gene="priC"
                     /locus_tag="ACHUZQ_001963"
     CDS             complement(9165..9692)
                     /gene="priC"
                     /locus_tag="ACHUZQ_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1025669.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9762..10139
                     /locus_tag="ACHUZQ_001964"
     CDS             9762..10139
                     /locus_tag="ACHUZQ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1025670.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10292..10843
                     /gene="apt"
                     /locus_tag="ACHUZQ_001965"
     CDS             10292..10843
                     /gene="apt"
                     /locus_tag="ACHUZQ_001965"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1025671.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10972..12897
                     /gene="dnaX"
                     /locus_tag="ACHUZQ_001966"
     CDS             10972..12897
                     /gene="dnaX"
                     /locus_tag="ACHUZQ_001966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1025672.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMPAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12230..12294
                     /gene="dnaX"
                     /locus_tag="ACHUZQ_001966"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12950..13279
                     /locus_tag="ACHUZQ_001967"
     CDS             12950..13279
                     /locus_tag="ACHUZQ_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1025673.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13279..13884
                     /gene="recR"
                     /locus_tag="ACHUZQ_001968"
     CDS             13279..13884
                     /gene="recR"
                     /locus_tag="ACHUZQ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1025674.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13994..15868
                     /gene="htpG"
                     /locus_tag="ACHUZQ_001969"
     CDS             13994..15868
                     /gene="htpG"
                     /locus_tag="ACHUZQ_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1025675.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16049..16693
                     /gene="adk"
                     /locus_tag="ACHUZQ_001970"
     CDS             16049..16693
                     /gene="adk"
                     /locus_tag="ACHUZQ_001970"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1025676.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            16825..17787
                     /gene="hemH"
                     /locus_tag="ACHUZQ_001971"
     CDS             16825..17787
                     /gene="hemH"
                     /locus_tag="ACHUZQ_001971"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1025677.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17784..18743)
                     /gene="aes"
                     /locus_tag="ACHUZQ_001972"
     CDS             complement(17784..18743)
                     /gene="aes"
                     /locus_tag="ACHUZQ_001972"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR1025678.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18895..20199
                     /gene="gsk"
                     /locus_tag="ACHUZQ_001973"
     CDS             18895..20199
                     /gene="gsk"
                     /locus_tag="ACHUZQ_001973"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1025679.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20332..22008)
                     /gene="ybaL"
                     /locus_tag="ACHUZQ_001974"
     CDS             complement(20332..22008)
                     /gene="ybaL"
                     /locus_tag="ACHUZQ_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1025680.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22246..>22335)
                     /locus_tag="ACHUZQ_001975"
     CDS             complement(22246..>22335)
                     /locus_tag="ACHUZQ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025681.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5439 a         5921 c         5855 g         5120 t
ORIGIN      
        1 ttttatgaat tttatccgtg gttaatactg gttttcgtat gagatcctga gttggtggtt
       61 caattactcc ttaatgttcg taggttatgc ataaaaaagg ccgcttgcgc ggccttagtg
      121 attacacgtt gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc
      181 ggctaaagcg gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg
      241 ttgcggtcac catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag
      301 caccagtact gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga
      361 tcggacgtaa acgcatccgc actgcatcaa gcgtcgcttc aatcagacct ttaccttctt
      421 tatccatcaa gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa
      481 tggttgtgag caggcctacc tggaagtaaa cgtcattggt caggccacgg aaggtggcag
      541 ccagcaacgc accgataacc cccagcggaa cgaccagcat aacggagaac ggaatcgacc
      601 agctctcgta cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg
      661 aaggtgcctg gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa
      721 caccggtagg cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt
      781 tacccggtgc cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac
      841 gcggcgaacc gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat
      901 cagcagcacg aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg
      961 acatgacgta aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc
     1021 cccatgcagc gcccagagag gtgttaatgt cgttgataga aacacccagc gcctgcgctt
     1081 tttcctggtc gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc
     1141 tggtcagcat atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca
     1201 gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac
     1261 ccagttccac gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga
     1321 aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg
     1381 cccagtcctt caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga
     1441 agccgttaac ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg
     1501 ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca
     1561 tggtcataaa tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca
     1621 gataggccat gccgaccacg atgatcagat acagcaccag gtaacgcccc gtactgcgca
     1681 gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttaaacc
     1741 agccgaagaa gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg
     1801 tggcacaaag agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa
     1861 caatggtaat agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg
     1921 gtacgaatac cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca
     1981 tcgacttacg ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt
     2041 tttctaccac aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca
     2101 ttgttagcgt gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata
     2161 ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca
     2221 taaccaggaa cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga
     2281 ttttcacgaa cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg
     2341 gttccatctt cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg
     2401 tcgccagctt gatccccaga ccggaagccg gttggccgtt aaactctgcg atgatgtcgt
     2461 agttctcacc acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct
     2521 gattcacttt cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa
     2581 taatagaggc gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg
     2641 caacctgggc gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt
     2701 tgttcagctc attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa
     2761 catcacccac gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag
     2821 agatatcctc ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc
     2881 tgctggatga tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca
     2941 tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact
     3001 caaaggtcag ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca
     3061 ggttatcgat accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt
     3121 tcgcatcagc gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat
     3181 attgcgccac cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga
     3241 tcacccacgc aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt
     3301 ttaagttaag acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca
     3361 gcggtaactt cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt
     3421 actacgcgat cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg
     3481 cttgcaacga tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca
     3541 tcaccacgcg gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc
     3601 ccttcttcca gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg
     3661 aagatagcgc gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc
     3721 agcgtaccgt cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg
     3781 ccgttctctt gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag gaagtcgttg
     3841 ctggactggg tcacatcaag gtagatcgga tcaagttgct gcacggttgc cagcgcagtc
     3901 gcctgaccgt tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta
     3961 atcggagagg tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc
     4021 gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtactcttgc
     4081 ttactgatgt actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata
     4141 ttggctgcag cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag
     4201 gtcgcaggat caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa
     4261 ttacgcttca ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg
     4321 gtgcgacccg gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca
     4381 acggcgggca tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct
     4441 aagctgcctg agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata
     4501 tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg
     4561 ctgcgtttat attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac
     4621 gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc
     4681 aacacatcct cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc
     4741 tgggcgagat tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag
     4801 acaagtcgga tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag
     4861 agcttgagta tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa
     4921 ttcatgttct tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat
     4981 tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct
     5041 gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt
     5101 tgcctgcgga tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc
     5161 tgatggaaaa ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt
     5221 acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta
     5281 acgaataacc ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct
     5341 ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag
     5401 aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt
     5461 tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg
     5521 tcatcgaatg gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat
     5581 aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta
     5641 gccaccctcg ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc
     5701 gctgaagcgc cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc
     5761 gataacgacg aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact
     5821 cgcgttgccc aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac
     5881 agccagctgg tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct
     5941 tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc
     6001 ttacgtccca gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt
     6061 gaccagcagc gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa
     6121 cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa
     6181 gcggtaaaca gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg
     6241 gatgaagccg cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag
     6301 cagttaagtc agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac
     6361 attaaagtca aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag
     6421 attgccgtcc tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg
     6481 ctgccctcgg cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa
     6541 cagtttgaag ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa
     6601 ctggaagaag gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg
     6661 gttgatatgc gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg
     6721 atggccatta acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa
     6781 tccatcctga ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg
     6841 atcaaagcgt tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac
     6901 tgggaaaaag cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg
     6961 attgccgggc tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct
     7021 tccgctgtgg gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc
     7081 gacctgatcc gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg
     7141 accatgcagc tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc
     7201 tggctggtgt ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac
     7261 ttcggcatgc cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc
     7321 gcgttgctgc ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg
     7381 gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta
     7441 tggccaatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actggtcacc
     7501 attaccgtgc tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc
     7561 tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg
     7621 aacttgctct accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg
     7681 cgtcgtgcgc tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg
     7741 ccggaagaac ccaccattgc actggaacag gttaaccagc agacgctgcg tattaccatg
     7801 ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc
     7861 gtgttcagct atctcgacag catcacgctc tggcattaca acggcactga agctggcgct
     7921 gcggtggtga aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg
     7981 gcctgggcgt tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg
     8041 aatatgcgtc agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc
     8101 gttggtgcga tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg
     8161 gccgcagcat tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc
     8221 tccggtttga tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt
     8281 agcttctcgg ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat
     8341 cgcaaagaag tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg
     8401 ttgactgaca ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg
     8461 gaaaaagtgc gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa
     8521 ccaatgccgg aagtcttctt tacggcgttt ggtgccagca cgttggatca tgagctgcgt
     8581 ctgtatgtgc gtgaactgcg tgaccgtagt cgtactgtcg atgagctgaa ccgtactatc
     8641 gatcagctgt gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg
     8701 cacaacgaga agggcgatga ggtgacggaa gtcaaacgcg actacaaagg cgatgacccg
     8761 acgccagcgg tagggtaata accaaagggg gcgacattta gttgccccga gattactgac
     8821 aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc
     8881 aagaattcaa tgtattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt
     8941 ttgcgtttgc ccacaatctt aggggacatt tagcggcccc atttatttct cacttttccg
     9001 cctcatcatc gcgcgttaat ttcttttcat aatcgcgttt tacaatatcc agcgcgcgca
     9061 gcacggtgcg ggctgggatc tgattttctt ccagcagcac aatcaaatcg acagccagtt
     9121 tgacatcgtc aggggcattt tcaagtgaca tactctctcc attgctagcg ggttaagcgc
     9181 gctagcctgt tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag
     9241 gcttccactt cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg
     9301 gctctgcgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt
     9361 ttacgctgcc agcgggcaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg
     9421 gaggcttcac gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact
     9481 tgcggcagct gttgctgctc aaccgcatga caaagcgcag ccagattatc gcccgcctcg
     9541 tcgagacaag cctgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca
     9601 cttagcgtgg cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct
     9661 tccagttttt ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg
     9721 ttatgctaaa gtagccactc tgtaagctga caacactcaa tatgcaacga atcattttaa
     9781 tcatcattgg ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac
     9841 cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc
     9901 acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc
     9961 atgcgatgcc gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca
    10021 tttctctgtg gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct
    10081 gtttgctttt ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa
    10141 gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca
    10201 caggcgcagg cggtcaaagg ttaaacaact gttacttttg atacgtttaa aacgcgccgt
    10261 gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta
    10321 tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga
    10381 tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga
    10441 gcgttacaaa aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt
    10501 tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa
    10561 actgccgcgt gaaaccatca gtgaaactta cgacctggaa tacggcaccg atcagctgga
    10621 gatccacgtt gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc
    10681 aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc
    10741 tgacgctgcg ttcattatca acctgttcga tctcggcggc gaacagcgtc tcgaaaaaca
    10801 gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg
    10861 ctgcccacgc tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt
    10921 gttagcatta ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat
    10981 caggtcttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat
    11041 gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt
    11101 tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac
    11161 tgcgaaaccg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag
    11221 caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat
    11281 acccgcgacc tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat
    11341 ctgatcgacg aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt
    11401 gaagagccgc cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg
    11461 gtgacgattt tgtcgcgctg tttgcaattt catctcaagg cgctggatgt cgagcaaatt
    11521 cgccatcaac ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc gcgggcgttg
    11581 caattgctgg cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag
    11641 gcgattgcca gcggtgacgg ccaggtttca acccaggcag tcagtgcgat gctgggtacg
    11701 cttgatgacg atcaggcgct gtcgctggtt gaagcgatgg tcgaggccaa cggcgagcgc
    11761 gtaatggcgc tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg
    11821 gaaatgctcg gcctgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc
    11881 aacgacatgc ccgccattga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg
    11941 gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg
    12001 gaccgccgca tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg
    12061 ccgctgcctg agccagaagt gccacgccag tcctttgcac ccgtcgtgcc aacggcagta
    12121 atgacgccaa cccaggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc
    12181 ccggaaacca ccagccaggt gctggcggcg cgccagcagt tgcagtgcgt gcagggagca
    12241 accaaagcaa aaaagagtga atcggcagcc gctacccgcg cgcggccggt gaataacgct
    12301 gcgctggaaa gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg
    12361 ctggaaaaag cgtcagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg
    12421 cagcaaaaag aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa
    12481 acgccggaac tggcggcgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca
    12541 caggtgagcc aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa
    12601 gaggagagcg acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac
    12661 cgcggtgcac agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa
    12721 ctgactatcg ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg
    12781 atatacgaag aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag
    12841 accctgcgtc ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg
    12901 taagcacagc ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa
    12961 aggcggtctg ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat
    13021 gcaggaagag atcgcgcagc tggaagtcac cggcgaatct ggcgcaggtc tggtaaaagt
    13081 gaccatcaac ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga
    13141 cgacaaagag atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat
    13201 tgaagaaacg cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg
    13261 ctttaagatg ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg
    13321 cgctgtctgc cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag
    13381 cgcgatcgta gcggcgggat gcgtttggcg caggcgctca cccgggcgat gtcggaaatc
    13441 ggccactgcg ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat
    13501 ccgcgtcgtc aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc
    13561 attgagcaga cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg
    13621 ctggacggca tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag
    13681 gaaaaaatca ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct
    13741 aactacattg ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc
    13801 gtaccggttg gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctcccttgcc
    13861 gggcgtcata agattcgttt ttaagcaaac gagagcagga tcacctgctc tcgcttgaaa
    13921 ttattctccc ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt
    13981 gaggtagacc tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct
    14041 tctgcacctg atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat
    14101 ctctaacgcc tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct
    14161 gtacgaaggt gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct
    14221 gaccatctct gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac
    14281 tatcgctaaa tccggtacca aatcattcct cgaatccctg ggttctgacc aggcgaaaga
    14341 cagccagctg atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa
    14401 agtgaccgtg cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc
    14461 ggctggcgaa ggtgaatata ccgttgccga catcaccaaa gaagatcgtg gtactgaaat
    14521 caccctgcat ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat
    14581 catcagcaaa tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa
    14641 agacggcgaa accgttatct cctgggagaa aatcaacaaa gcgcaggcgc tgtggactcg
    14701 taacaagtcg gaaatcaccg atgaagagta caaagagttc tacaaacaca tcgcccacga
    14761 ctttaatgat ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag
    14821 cctgctgtac attccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg
    14881 cctgaaactg tacgtccagc gtgtgttcat catggacgac gcagaacagt tcatgccgaa
    14941 ctatctgcgc ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg
    15001 tgaaatcctc caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt
    15061 gctgcaaatg ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca
    15121 acagtttggc ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat
    15181 cgccaaactg ctgcgttttg cttctaccca taccgattct tctgcgcaga ccgtatctct
    15241 ggaagactac gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcaccgcaga
    15301 cagctatgcg gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga
    15361 agttctgctg ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga
    15421 cggtaaaccg ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga
    15481 agttgatgag agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgtgtgaa
    15541 agccctgctc ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc
    15601 agcgatcgtt tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc
    15661 ggcgggccag aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact
    15721 ggtgaaacgt gcggcagata ctgaagatga agcgaagttc agcgagtggg tagaactgct
    15781 gctggatcag gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg
    15841 tcgtatgaac cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc
    15901 atttttcttt tcatcatctg cactttccgc aaattatctc gccattaacc gtttcagcct
    15961 caggtgcctt tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa
    16021 atcgacacat tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc
    16081 ggggaaaggg actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac
    16141 tggcgatatg ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga
    16201 cattatggat gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg
    16261 cattgctcag gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc
    16321 gcaggcagac gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt
    16381 accggacgaa ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg
    16441 tgtttatcac gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga
    16501 agaactgact acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata
    16561 ccatcagatg actgcaccgc tgatcggcta ctacttcaaa gaagcggaag cgggtaacac
    16621 caaatacgcg aaagttgacg gcaccaagtc ggttgctgaa gttcgcgctg atctggaaaa
    16681 aatcctcggc taatcaagcc gcagcagatc tgctttcggg caggtctgct catccacctc
    16741 aggcaatacc tctcacagca attagttctt cttcctcact tttccgctac aattatcaac
    16801 aagttgaatc gataagaggc ggtaatgcgt cagactaaaa tcggtatcct gctggcaaac
    16861 ctgggtacgc ccgatacccc cacacctgaa gcggtaaaac gctatctgaa acaattttta
    16921 agcgacagac gcgtggttga tacctcacgg ttgttatggt ggccattgct gcgcggcgtt
    16981 attttgccgc tgcgctcgcc gcgtgtggcg aagctgtacg cctcggtctg gatggaaggt
    17041 ggctcgccgc tgatggttta cagccgccag caacagcagg cgctggcaca acgtttaccg
    17101 gagacgcccg tagcgctggg aatgagctac ggctcgccat cactggaaag cgccgtcgat
    17161 gaactcctgg cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc
    17221 tgttctacgg tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc
    17281 attccgggga tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg
    17341 gcgaacagcg tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct
    17401 tatcatggca ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc
    17461 acaacgactc gcgaactggc ttccgcactg gggatggcac cggaaaaagt gatgatgacc
    17521 tttcagtcgc gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa
    17581 atgctcggag aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat
    17641 tgtctggaga cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc
    17701 gggaaaaaat atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg
    17761 gctaatcttg ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga
    17821 agagcctcgt cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct
    17881 gggtagagtt tgaactcaca gggctgctga tgcgccgcta acgtctggta aagcagacgg
    17941 ctgtcatcca gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga
    18001 gtgagatcat tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa
    18061 tacgcctctt catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac
    18121 agacgacgag tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca
    18181 attttaccgc aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg
    18241 gcacctgcgg aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc
    18301 tgctggtgga aataacaaca agcagccaca atttcctcta tcgcttgcgg aaaacgcgct
    18361 tcaggtgaaa gggtgtaatc aataccaatc accgtacatt ggctgtagct tgccagcagg
    18421 cgcatgatgc gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa
    18481 aatagcgtcg ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat
    18541 tttgttggaa ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg
    18601 cgctcaagcg tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt
    18661 aaatccggct gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca
    18721 ggtagtttgt tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac
    18781 cactgccatg aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct
    18841 cccgtcagat tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa
    18901 tttcccggta aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt
    18961 cagcaattcc agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg
    19021 ctggtcgata ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc
    19081 gggcattcac tggtgattga ggatgacgta gccgaagcgc tttatcagga gctaaaacag
    19141 aaaaacctga ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac
    19201 tcggtgctcg cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt
    19261 ggcagttatg cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta
    19321 caaggcgtgg atggcccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt
    19381 acctttgcta tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat
    19441 gtgattgccg gagcctcggc actggttctc acctcttatc tggtgcgttg caaaccgggt
    19501 gaacccatgc cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg
    19561 gtggtgctga cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa
    19621 ttcctcaaag atcacgtctc tatccttgcg atgaacgaag atgaagccga agcgttgacc
    19681 ggagaaagcg atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg
    19741 tgcaccgccg ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt
    19801 aaaacccagc atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc
    19861 cgcgccatgc gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg
    19921 tacatgggcg ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca
    19981 gcgttgctgc atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc
    20041 aaacataaat tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac
    20101 cgtgtgagct atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag
    20161 cgtgaagaca gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc
    20221 agataaggcg ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg
    20281 cgtcttatca tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt
    20341 caccacttca cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg
    20401 ttcacccatc actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata
    20461 atgggcgcgg gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc
    20521 acccgcttca taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgcgc
    20581 cagttgcata atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg
    20641 cagctcatca acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag
    20701 caatttctcc cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca
    20761 aatatccact gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag
    20821 cgtttcggtc ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat
    20881 cgacaggatc gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc
    20941 cattcccagt cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat
    21001 ggttaatgcc gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga
    21061 tttaccaaac agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat
    21121 taacggatca aacaacatcc cgacggagac aaaaaacagc accgcaaacg cgtcgcgcaa
    21181 tggcagcgta tcgtgggcgg cacggtgact cagttcagac tcgttcagta ccatcccggc
    21241 aaagaacgcg ccaagtgcga aggagacgtc aaacaactcc accgcaccaa aggcaatccc
    21301 taacgccagc gccagcaccg acagggtaaa tagctcgcga gaaccggttg ccgcgctgcg
    21361 tgccataatc cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat
    21421 cactttgccg atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg
    21481 ttccatcatt cctgccactg cgggcagcaa caccagcgtc agaaccatta ccaggtcttc
    21541 cacaatcaac caaccgatgg cgatttgccc acgctgactg tcaattaatt gccgttcttc
    21601 aagtgcgcgc agtaacacca cggtactggc ggtggaaaga cataaaccga acacgatacc
    21661 ggtcattaac gaccagccca gcacggcaga gagcgccata cccagcagcg tcgccacggc
    21721 tatctgggcg atcgcaccgg gaatggcgat ggcctttacc gccatcaaat ccttcagcga
    21781 aaagtgcaaa ccgacgccaa acatcaacag aatgacgccc agttcagcca gttccggcgc
    21841 aagcttggta tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag
    21901 atatcccacc agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag
    21961 cacaaggccg ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt
    22021 tcctggtggt tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg
    22081 acaatttcat tgatgatgtt catgaataat tattgaattt tgcagaaaaa tggaattagc
    22141 tgcaaaaaaa gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg
    22201 actgtaaaac gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg
    22261 gttatcaggc aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata
    22321 gactaactcg atgct
//
LOCUS       DAZPBM010000056        22241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPBM010000056 DAZPBM010000000
VERSION     DAZPBM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.056"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUZQ_001976"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUZQ_001976"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR1025682.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHUZQ_001977"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHUZQ_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1025683.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHUZQ_001978"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHUZQ_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1025684.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHUZQ_001979"
     CDS             2284..2730
                     /locus_tag="ACHUZQ_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1025685.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHUZQ_001980"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHUZQ_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR1025686.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="ACHUZQ_001981"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="ACHUZQ_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR1025687.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="ACHUZQ_001982"
     CDS             4841..5188
                     /locus_tag="ACHUZQ_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1025688.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHUZQ_001983"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHUZQ_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1025689.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHUZQ_001984"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHUZQ_001984"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1025690.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="ACHUZQ_001985"
     CDS             6705..6803
                     /locus_tag="ACHUZQ_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1025691.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHUZQ_001986"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHUZQ_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1025692.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="ACHUZQ_001987"
     CDS             complement(6939..7187)
                     /locus_tag="ACHUZQ_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1025693.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="ACHUZQ_001988"
     CDS             complement(7335..7517)
                     /locus_tag="ACHUZQ_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR1025694.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHUZQ_001989"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHUZQ_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR1025695.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHUZQ_001990"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHUZQ_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR1025696.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHUZQ_001991"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHUZQ_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR1025697.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHUZQ_001992"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHUZQ_001992"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR1025698.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHUZQ_001993"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHUZQ_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR1025699.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHUZQ_001994"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHUZQ_001994"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR1025700.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHUZQ_001995"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHUZQ_001995"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1025701.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHUZQ_001996"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHUZQ_001996"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1025702.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHUZQ_001997"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHUZQ_001997"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1025703.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHUZQ_001998"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHUZQ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1025704.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHUZQ_001999"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHUZQ_001999"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1025705.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21394)
                     /locus_tag="ACHUZQ_002000"
                     /pseudo
     CDS             complement(19482..21394)
                     /locus_tag="ACHUZQ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     gene            21526..21870
                     /locus_tag="ACHUZQ_002001"
     CDS             21526..21870
                     /locus_tag="ACHUZQ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1025706.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5450 a         5699 c         5401 g         5691 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cacccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aagaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattagt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcacaaggaa acgaaccgca cgggcaatgt cacgggtgcg tggcgcgggc ggcagactgg
    19561 cgagaaacgt cgcgccgtaa ggacgcatca ccagccgatt gtcacaaatt accagcacgc
    19621 cgcgatcgtc ggcgtcgcga atcagtcgcc ctactccctg cttgagagta atgaccgcat
    19681 ccggcagctg cacttcatcg aacgggtcgc caccgcgcaa acgacaatct tccatgcgcg
    19741 cttttaacag tagatcatcc ggcgaggtaa acggcaattt gtcgataatt accaatgaca
    19801 atgtatcgcc acgcacgtcc accccttccc agaaactact ggttgccaca agaagcgcgt
    19861 taccggcgct gacaaattgc tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa
    19921 cgggaagcgt catggtagcg cggaactgct cggccagatc gcgcatcatg gcatgcgagg
    19981 tacaaagcat aaaacaacga ccgttgttag cttcgatgat cggtcgcagc attgccgcca
    20041 gttgccgtgc acaacccggt tgattggttt gcggcaggtt gcgcggcaca cagagtaacg
    20101 cctggcggct gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa
    20161 gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc
    20221 agctaccggg cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga
    20281 gagccagagt aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga
    20341 tctcttttag ccgcttcagc cgtgtgcgat acaacgtggc gcgctcaaat gccgcatcca
    20401 gcaaggcgga acgccccagt gacagtttcg ccacgtcata acaaagttcc agggtgtcat
    20461 cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc
    20521 gataacctgg ctcaccgagt tgcagacgaa aatcctgtgc actctgggca agacgatcag
    20581 cgcacttttg taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt
    20641 ttgccaggtc gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt
    20701 ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc ttcagggatc aactcgccaa
    20761 atccactctc tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat
    20821 cggcgtccat cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt
    20881 cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg ccacgcctgt gaatcttccg
    20941 ccacgctgac gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca
    21001 ggatcacatc gcttaagatt tgtaccggca gatcgccccc cgccagcgcc tgctgttcga
    21061 gacgttcgag gcagaggtag tttgagcgcc ctttcagcag cgccacgttg cccgtatatt
    21121 tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg
    21181 agccggtcga gataatgact ttctttttcg cccgcagcgc aggagccagg taagcgtagg
    21241 ttttgcccgt accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg
    21301 tgacggctac cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg
    21361 ccagctgacc gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacaggg
    21421 attatgtcag gatgagggcg ctttcgccag ttgaagtggt gacggcaacc tcacattgtg
    21481 gcagtctttg cagcacgaaa atggaagtat aatgaggaca aaatgatgac tatcgttcgt
    21541 atcgatgctg aagcccgctg gtctgatgta gtaatccaca acaacacgct ctactacact
    21601 ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa ccgccaacac gctggcacag
    21661 attgacgccg tgctggaaaa acagggcagc aataaatcga gcattctgga tgccaccatt
    21721 ttcctggccg ataaaaacga cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc
    21781 gcgggtcatg cgccagtgcg ctgcacggta caagcgggtt tgatgaaccc gaagtataaa
    21841 gttgaaatta agattgtggc tgcggtgtaa gctttatcga agcaaaataa gtcagacgat
    21901 aatttatcga taatactggt cggttttaca taaatcgacc agagaataag atattactgt
    21961 tgatgcttct gctaattcat tatttatatt tataatttca attttatcta taaaactaac
    22021 tatataaata aacataacca tgccgttgtg ttcgcccttt gattagcaac tctggttttc
    22081 tttcttagct gaacaatctg gttatagcgt gcggactgac cgttaatttt ctccttctta
    22141 ctggataaca caccatgagc tgctaccttt aaaacacctg aatgtcaaca ggttaactcg
    22201 tgccatatcg ttataatccc tttgatatta gatgcaaatt a
//
LOCUS       DAZPBM010000057        21955 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPBM010000057 DAZPBM010000000
VERSION     DAZPBM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.057"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..989)
                     /gene="cmtA"
                     /locus_tag="ACHUZQ_002002"
     CDS             complement(<1..989)
                     /gene="cmtA"
                     /locus_tag="ACHUZQ_002002"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR1025707.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1017..1460)
                     /gene="cmtB"
                     /locus_tag="ACHUZQ_002003"
     CDS             complement(1017..1460)
                     /gene="cmtB"
                     /locus_tag="ACHUZQ_002003"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR1025708.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1774..3765)
                     /gene="tkt"
                     /locus_tag="ACHUZQ_002004"
     CDS             complement(1774..3765)
                     /gene="tkt"
                     /locus_tag="ACHUZQ_002004"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1025709.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4043..4801
                     /gene="loiP"
                     /locus_tag="ACHUZQ_002005"
                     /gene_synonym="yggG"
     CDS             4043..4801
                     /gene="loiP"
                     /locus_tag="ACHUZQ_002005"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1025710.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4944..5864)
                     /gene="speB"
                     /locus_tag="ACHUZQ_002006"
     CDS             complement(4944..5864)
                     /gene="speB"
                     /locus_tag="ACHUZQ_002006"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1025711.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6002..7978)
                     /gene="speA"
                     /locus_tag="ACHUZQ_002007"
     CDS             complement(6002..7978)
                     /gene="speA"
                     /locus_tag="ACHUZQ_002007"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1025712.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7987..8118)
                     /gene="yqgB"
                     /locus_tag="ACHUZQ_002008"
     CDS             complement(7987..8118)
                     /gene="yqgB"
                     /locus_tag="ACHUZQ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1025713.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8212..8355)
                     /gene="yqgG"
                     /locus_tag="ACHUZQ_002009"
     CDS             complement(8212..8355)
                     /gene="yqgG"
                     /locus_tag="ACHUZQ_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1025714.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8466..8717)
                     /gene="yqgD"
                     /locus_tag="ACHUZQ_002010"
     CDS             complement(8466..8717)
                     /gene="yqgD"
                     /locus_tag="ACHUZQ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1025715.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8773..9927
                     /gene="metK"
                     /locus_tag="ACHUZQ_002011"
     CDS             8773..9927
                     /gene="metK"
                     /locus_tag="ACHUZQ_002011"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1025716.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10363..11757
                     /gene="galP"
                     /locus_tag="ACHUZQ_002012"
     CDS             10363..11757
                     /gene="galP"
                     /locus_tag="ACHUZQ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1025717.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11834..12331
                     /locus_tag="ACHUZQ_002013"
     CDS             11834..12331
                     /locus_tag="ACHUZQ_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1025718.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12426..13133
                     /gene="endA"
                     /locus_tag="ACHUZQ_002014"
     CDS             12426..13133
                     /gene="endA"
                     /locus_tag="ACHUZQ_002014"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1025719.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13213..13944
                     /gene="rsmE"
                     /locus_tag="ACHUZQ_002015"
     CDS             13213..13944
                     /gene="rsmE"
                     /locus_tag="ACHUZQ_002015"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1025720.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13957..14907
                     /gene="gshB"
                     /locus_tag="ACHUZQ_002016"
     CDS             13957..14907
                     /gene="gshB"
                     /locus_tag="ACHUZQ_002016"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1025721.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15016..15579
                     /locus_tag="ACHUZQ_002017"
     CDS             15016..15579
                     /locus_tag="ACHUZQ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1025722.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15579..15995
                     /gene="ruvX"
                     /locus_tag="ACHUZQ_002018"
     CDS             15579..15995
                     /gene="ruvX"
                     /locus_tag="ACHUZQ_002018"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1025723.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16170..17150)
                     /locus_tag="ACHUZQ_002019"
     CDS             complement(16170..17150)
                     /locus_tag="ACHUZQ_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1025724.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17168..17872
                     /gene="yggS"
                     /locus_tag="ACHUZQ_002020"
     CDS             17168..17872
                     /gene="yggS"
                     /locus_tag="ACHUZQ_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1025725.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17890..18456
                     /gene="yggT"
                     /locus_tag="ACHUZQ_002021"
     CDS             17890..18456
                     /gene="yggT"
                     /locus_tag="ACHUZQ_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1025726.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18453..18743
                     /gene="yggU"
                     /locus_tag="ACHUZQ_002022"
     CDS             18453..18743
                     /gene="yggU"
                     /locus_tag="ACHUZQ_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1025727.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18751..19344
                     /locus_tag="ACHUZQ_002023"
     CDS             18751..19344
                     /locus_tag="ACHUZQ_002023"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1025728.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19337..20473
                     /gene="hemW"
                     /locus_tag="ACHUZQ_002024"
     CDS             19337..20473
                     /gene="hemW"
                     /locus_tag="ACHUZQ_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1025729.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDNALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20740..21726
                     /locus_tag="ACHUZQ_002025"
     CDS             20740..21726
                     /locus_tag="ACHUZQ_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1025730.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            21771..>21955
                     /locus_tag="ACHUZQ_002026"
     CDS             21771..>21955
                     /locus_tag="ACHUZQ_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1025731.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
BASE COUNT         5426 a         5709 c         5590 g         5230 t
ORIGIN      
        1 atagcaaagg acaccagggt agctaccgta acaccggcaa ttgtcgccag gaacgagcct
       61 ttcggcgtca gtgccaggta agcaaagata gaccccggac ttgggccagc caccagacca
      121 ccgtccagta agttaaacac ccaggtgcca gacataccgc ccgcaatcat ggcaataatg
      181 gtcagcggct tcatcagcac atacgggaaa tacagttcgt ggatcccacc gaggaagtga
      241 ataatcatcg ccccgggcgc agaacgttta ctcatccctt taccaaacaa ggtaaacgcc
      301 agcagcagcc ctaagccagg cccaggattg gacgccacca taaagaagat ggatttaccg
      361 ttaaccgaag cctgttgcat tcccagcgga taatagacgc cctgatcgat cgcattattg
      421 aggaaaagca ctttcgccgg ttcattgatg actgacagca gaggcagata acccgcatgt
      481 accagcgcct caatacactc tttgacgaga gtattggcaa ttaacaccgc cgggccgata
      541 acttcaaaac ccagcagaca aaggagcatc cccgcgatac ctaatgagaa gttattgata
      601 accatctcaa aaccggcagg tatgcgtttt tccagtgcct tatcaacata ttttatgacc
      661 agcccaccga gcggccccat aatcattgag ccaaggaaca tcgggatctc tgcgccgacg
      721 atcacaccaa tagtacctat tccgcccatg accgcgccgc gtttaccgcc gaccagatga
      781 ccaccggtag aaccaatcat cacgggcaat aaataggtaa tcatcgggcc gacaattttg
      841 gcgaaatgtt cgttaggcag ccaaccggtg ggaataaata acgcagtaat aaaaccccag
      901 gcaataaaag caccaatatt ggggatgacc attgccgtca aaaagccccc aaaagcctgg
      961 acctttgcac gagcagactt gttttccata atattatcct gtagaggaga gagtaattat
     1021 ccgcggctga ttatgtccgc taattgtttt tctgatgctg ctgttaagag ttgttcgagt
     1081 atttcttctt cgcacagtaa ttcacttaac gcctgaatag cgccaatgtg cgaatcggca
     1141 tcggcagcag aaaggccgat gagtaatttt attggttcgt cattccccgg aaaataaaca
     1201 ccttgttcaa gtaatgtcaa agacatcccg gttttaagcg ccccacattc cggtcgcgca
     1261 tgaggcattg ccacgcctgg tgcgagaata taataagggc cattgttaat ggtggaatct
     1321 ttaattgcct gaatgtaatt ctcgctgata tagtttttat ccagcaatga taccatcgag
     1381 aaatcgatag cttcctgcca atcttttgct gaatgtataa ctgagattga tgattctgga
     1441 aaatagtcac taagccgcat aaattatcct tattttattt ttagggtacc gtccggcggt
     1501 aaaaataaaa tactcgtgcg gtttaatgaa agcaattgag cagggaaacc ggtgttttga
     1561 aaacagtgaa ttattaattt cttatataac attgagttat agatataaca acaaatgtca
     1621 atacgcatat cgtgatgcat attacataat tgatatttat tgcatataaa tatttgtgat
     1681 ctacaacacg ctttatctat tgcttgtccg caaacggaca tatcaaggta ataaaaaagg
     1741 tcgccgaagc gaccttttta cccgaaatgc taattacagc agttcttttg ctttcgcaac
     1801 gacgttgtcg acggtgaagc cgaactcttc gaacagcaac tctgccggag ctgactcacc
     1861 gaaggtggtc ataccgatga tagcgccatt caggccaaca tacttgtacc agtagtcagc
     1921 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1981 agccgcatcc tgcttgtcga atgcatcggt agacggcatg gaaaccacgc gcgccttcac
     2041 gccttcggca gtcagttttt cgtaggcggc aacagccagt tcaacttccg aaccggtcgc
     2101 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     2161 gtttgccagt tgctcttcag tacgttcctg ctgcgccaga ttctgacggg agaggataag
     2221 cgcggtcgga ccgtcctgac gctcaacacc gtatttccac gcgaccgcgg attcaacctg
     2281 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctcaac
     2341 cggctggtga gtcgggccgt cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     2401 ctgacgctgt ttcatcagcg cagccatacg tacagcgtta cgtgcgtatt cgacaaacat
     2461 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     2521 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cccgcagcat cttcgttgat
     2581 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcgg agccgccgag
     2641 gaattccggc aacagcgggc cgaacgcttc aatagcattc tgagacgctt tacggctggc
     2701 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2761 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2821 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2881 atcccactgc gcatagattt cagacgggat ttcgaacggt gcgtatttcc agcccagctg
     2941 ttcgcgggtc agagcgattt ctgcatcacc cagcggcgca ccgtgggagt cgtgagtacc
     3001 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     3061 agtcactgcg cgcgcttctt ctactgcgcg tttgatggat gctgcgtcat gaccgtcgat
     3121 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca
     3181 gccttcaacg tgaccatcaa tagagatacc gttgtcatcg tagaacgcaa tcagtttacc
     3241 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     3301 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     3361 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     3421 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     3481 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     3541 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     3601 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     3661 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3721 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3781 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3841 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3901 cagaaatctc tggatcatgc tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt
     3961 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     4021 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     4081 aacggtactg actggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     4141 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     4201 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     4261 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     4321 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     4381 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     4441 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     4501 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     4561 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     4621 tgattattct tacgatcttc tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag
     4681 ctttgaaaaa ctggcgaaac tggaagaagg ccgccaaagc tcaatgtttg acgaccatcc
     4741 ggcatccgcc gaacgcgccc agcatattcg cgatcgcatg agcgcggatg ggattaagta
     4801 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacatcaatg
     4861 gcacatttgt taccctgtgc gcattgccgg atgcgatgct gactgcatct tatccggcct
     4921 acgggtggca tcgcatctgg tgcttactcg ccctttttcg ccgcttggat atacagcatc
     4981 tccagcgcca gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc
     5041 acttccacta cgtccatccc aacaatgttg agatctttca ggccgcgtac cagtttaata
     5101 gcgcgatcgg aggtcaggcc gccaatcact ggcgtaccgg tgcctggtgc aaaagcagga
     5161 tccaggcagt cgatatcaaa agtcaggtaa accggcatat cacccacaat ctgtttcact
     5221 tgggcgataa cgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag
     5281 ccgttgtctt tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc
     5341 agaccttctt tcggcgcggt atagaacata gtgccgtggt caaattcaca accgttcgca
     5401 taggtatcgg tgtgggcgtc aaagtgtacc agcgccattt tgccgaaatg cttcgcatga
     5461 gcacgcagca gcggcagcgt aacaaagtgg tcaccaccga aggtgagcat acgcttaccg
     5521 gcagccagca gcttctcggc gtgcgcctgc agcttttcgc tcatctcacg ggcatcgcca
     5581 aaggcatata ccagatcgcc gcagtccacg acgttcagac gctcacgcat gtcgaaattc
     5641 cacgggaagc ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga
     5701 ccgtggcgac cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc
     5761 cagtctgcat cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggca
     5821 ttggaaacca gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta
     5881 caaagtcgat ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt
     5941 gtcacaataa attgtggcgg attatcaccg ctaaattaag cggattcaag taacacagga
     6001 cttactcatc ttcaagataa gtataaccgt acaaacctgc ctcgaactct tcaaggaact
     6061 gttgttgcag ttcagcatca agatcggttt tcttcacttg atcgcggaac tgggttaaca
     6121 gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg ccttcgtcag
     6181 acagttctac ttctacgcta ccgtcaggga acacgaacac gtcaaccgct tcggtatcac
     6241 cgaacaggtt gtgcatattg ccaaggatct cctgatatgc gccgaccata aagaaaccga
     6301 gcatcggcgg attctctgga tcgtactccg gcattggcat tgtcgtggca ataccatcac
     6361 catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc agcacagcgc
     6421 gacgttccgg cacttgatcc agcccttcca gcggcagaac cgggaacaac tggtcgatcc
     6481 cccatgcgtc cggcattgac tggaacagcg agaaattgac gtacattttg tccgccatac
     6541 gttcctgcag ttcgtcgatt atcggacgat gcgcacggtt ttgcggatcc agttgcttct
     6601 gcacttcatg gcacatgctc agatagagtt gttctgccca tgcacgttct tgcaggctaa
     6661 aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctggctg tcgtgtaacc
     6721 attcacgcaa agaacggcga gttcccggtt cgtgcatttc ctgccaggtt tcccacatgc
     6781 tttgcagcgc gcgcggcgca tcttctgcgg gcgcggtcgg caccgtatat tcgttacgtt
     6841 ccacgccgat gatattcgac accagcacgg tatggtgcgc agtcaccgca cggcccgatt
     6901 cggtgattac cgtcggatgc ggcagaccat tttcttcaca cgcatcgcca atcgcccaga
     6961 tgatgttgtt ggcgtattca ttgaggccat agtttacgga gcagtcagac tgcgaacggg
     7021 tgccttcata gtccacgccc agaccgccgc cgacgtcgaa gcactgaata ttgacgccca
     7081 gcttgtgcag ttccacatag aaacgcgcgg attcacgaac gcccgtggcg atatcgcgaa
     7141 tattcgccat ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg agacgcccgg
     7201 cttcacgcag ggtttcaacc agttgcagta cctgagtcgc agccaggccg aacttcgatt
     7261 tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt gcacgcacgc
     7321 ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg atttctgaca
     7381 tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat gccaggcgga
     7441 tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgggtcatg ccagcatgtg
     7501 ccagtactgc catcaactcg gctttggaac cggcttccag acccagcggt tcgcccgaat
     7561 gaatcaggga ctcaatcacg cggcggtgct ggttaacttt gatcggataa acaaggaagt
     7621 aatcgccgtt atagccgtag gattccctcg cacgtttgaa cgcggcgtta atggaacgca
     7681 aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga ccctgtgctt
     7741 cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc
     7801 acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac caggcaatat
     7861 tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc tgcatggagc
     7921 gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg tcagacatag
     7981 cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag cctggaactg
     8041 cgataacaac ccataagcac atggattttc cagcagtgaa tgctgacgct ccaactgcgc
     8101 aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg tgacagttcg
     8161 gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc ctcagtaact
     8221 gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc aggtatgcaa
     8281 agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca ggttaagcag
     8341 cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac gaacccacaa
     8401 gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt
     8461 tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg caaaagataa
     8521 atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa cagaatgaga
     8581 cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc catccagatg
     8641 ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacctg cagagtcgtg
     8701 gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa ttctctttag
     8761 gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga agggcatcct
     8821 gacaaaattg ctgaccaaat ctctgatgcc gttttagacg cgatcctcga acaggatccg
     8881 aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt tggcggcgaa
     8941 atcaccacca gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt
     9001 ggctatgtgc attccgacat gggctttgac gctaactcct gtgcggttct gagcgctatc
     9061 ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct ggaacagggc
     9121 gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt gctgatgcca
     9181 gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc
     9241 actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta tgacgacggc
     9301 aaaatcgttg gtatcgatgc tgtcgtgctt tccactcagc actctgaaga gatcgaccag
     9361 aaatcgctgc aagaagcggt aatggaagag atcatcaagc cgattctgcc cgctgaatgg
     9421 ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gttttgttat cggtggcccg
     9481 atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg cggcatggca
     9541 cgtcacggtg gcggagcatt ctctggtaaa gatccatcaa aagtggaccg ttccgcagcc
     9601 tacgcagcac gttatgtcgc gaaaaacatc gttgctgctg gcctggccga tcgttgtgaa
     9661 attcaggttt cctacgcaat cggcgtggct gaaccgacct ccattatggt agaaactttc
     9721 ggtactgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt cttcgacctg
     9781 cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa agaaaccgca
     9841 gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg
     9901 ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gctttcaaag gccagcctcg
     9961 cgctggcctt tttcttttac ctcaccgcgg tggataggca ttcacgccgc atccggcaaa
    10021 aagctgcccg cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac
    10081 tactgaaacc gattacacca ataacaccag ccaaatattt gtaatatttt catattatta
    10141 ttcagttttc acagttgtta catttctttt cagtaaagtc ttaattgcag ataacagcgt
    10201 ttaatctatg atgatataac tcaattattt tcatgcactt aaaccataac taagataaat
    10261 gttagtgtaa gcgattacac tgatgtgatt tgcttcacat ctttttacgt cgtactcacc
    10321 tatcttaatt cacaataaaa aataaccata ttggagggca tcatgcctga cgctaaaaaa
    10381 caggggcggt caaacaaggc aatgacgttt ttcgtctgct tccttgccgc tctggcggga
    10441 ttactctttg gcctggatat cggtgtaatt gctggcgcac tgccgtttat tgcagatgaa
    10501 ttccagatta cttcgcacac gcaagaatgg gtcgtaagct ccatgatgtt cggtgcggca
    10561 gtcggtgcgg tgggcagcgg ctggctctcc tttaaactcg ggcgcaaaaa gagcctgatg
    10621 atcggcgcaa ttttgtttgt tgccggttcg ctgttctctg cggctgcgcc aaacgttgaa
    10681 gtactgattc tttcccgcgt tctactgggg ctggcggtgg gtgtggcctc ttataccgca
    10741 ccgctgtacc tctctgaaat tgcgccggaa aaaattcgtg gcagtatgat ctcgatgtat
    10801 cagttgatga tcactatcgg gatcctcggt gcttatcttt ctgataccgc cttcagctac
    10861 accggtgcat ggcgctggat gctgggtgtg attatcatcc cggcaatttt gctgctgatt
    10921 ggtgtcttct tcctgccaga cagcccacgt tggtttgccg ccaaacgccg ttttgttgat
    10981 gccgaacgcg tgctgctacg cctgcgtgac accagcgcgg aagcgaaacg cgaactggat
    11041 gaaatccgtg aaagtttgca ggttaaacag agtggctggg cgctgtttaa agagaacagc
    11101 aacttccgcc gcgcggtgtt ccttggcgta ctgttgcagg taatgcagca attcaccggg
    11161 atgaacgtca tcatgtatta cgcgccgaaa atcttcgaac tggcgggtta taccaacacc
    11221 acagagcaaa tgtgggggac agtgattgtc ggcctgacca acgtacttgc cacctttatc
    11281 gcaatcggcc ttgttgaccg ctggggacga aaaccaacgc taacgctggg cttcctggtg
    11341 atggctgctg gcatgggcgt actcggtaca atgatgcata tcggtatcca ctctccgtcg
    11401 gcgcagtatt tcgccatcgc catgctgctg atgtttattg tcggttttgc catgagtgcc
    11461 ggtccgctga tttgggtact gtgctccgaa attcagccgc tgaaaggccg cgattttggc
    11521 atcacctgct ccactgccac caactggatt gccaacatga tcgttggcgc aacgttcctg
    11581 accatgctca acacgctggg taacgccaac accttctggg tgtatgcggc tctgaacgta
    11641 ctgtttatcc tgctgacatt gtggctggta ccggaaacca aacacgtttc gctggaacat
    11701 attgaacgta acctgatgaa aggtcgtaaa ctgcgcgaaa tcggcgctca cgattaatct
    11761 cctcaggctt cctcccgtcg cggaggaagc cgcctcttgc agtcatcttt tcttcgctct
    11821 atcctctgcc gctatgaaaa catcccgtct ccctatcgcc atccaacagg ccgttatgcg
    11881 cggcctgcgg gaaaaactcg cccaggccaa cctgaagcta gggcgtaact acccggagcc
    11941 aaaactctct tacacccagc gcggaacctc cgccggaaca gcctggctgg aaagctatga
    12001 aatccgcctc aatcccgttt tgctgttgga aaacagtgaa gcttttattg aagaagtggt
    12061 accgcacgaa ctggcacatt tgctggtatg gaaacatttc ggccgcgtag cgccacatgg
    12121 caaagagtgg aagtggatga tggaaagcgt gctgggtgtt cccgcccgtc gtacgcatca
    12181 gttcgaactg caatccgtgc gtcgcaacac cttcccctac cgctgcaagt gccaggagca
    12241 tcagcttacc gtacgccgcc ataatcgcgt agttcgtggc gaggccgtct atcgctgtgt
    12301 tcactgcggt gaacagctgg ttgcgaaata accatctgaa ctatcaggaa ctttcctgat
    12361 ctggctgatt gcataccaaa acaactttcg ctacgttgct ggctcgtttt aacacggagt
    12421 aagtgatgta ccgttatttg tctattgctg cggtggtact gagcgcagca ttttccggcc
    12481 cggcgttggc cgaaggtatc aatagttttt ctcaggcgaa agccgcggcg gtaaaagtcc
    12541 acgctgacgc gcccggtacg ttttattgcg gatgtaaaat taactggcag ggcaaaaaag
    12601 gcgttgttga tctgcaatcg tgcggctatc aggtgcgcaa aaatgaaaac cgagccagcc
    12661 gcgtagagtg ggaacacgtc gttcccgcct ggcagttcgg tcaccagcgc cagtgctggc
    12721 aggacggtgg acgtaaaaac tgcgctaaag atccggtcta tcgcaagatg gaaagcgata
    12781 tgcataacct gcagccgtca gtcggtgagg tgaatggcga tcgcggcaac tttatgtaca
    12841 gccagtggaa tggcggcgaa ggccagtacg gtcaatgcgc catgaaggtc gatttcaaag
    12901 aaaaagctgc cgaaccacca gcgcgtgcac gcggtgccat tgcgcgcacc tacttctata
    12961 tgcgcgacca atacaacctg acactctctc gccagcaaac gcagctgttc aacgcatgga
    13021 acaagatgta tccggttacc gactgggagt gcgagcgcga tgaacgcatc gcgaaggtgc
    13081 aaggcaatca taacccgtat gtgcaacgcg cttgccaggc gcgaaagagc taacctacac
    13141 tagcgggatt ctttttgtta acccctaccc cacgcgtaca accgcgtggg gagacgacgc
    13201 ggatttttaa ctatgcgtat cccccgcatt tatcatcctg aaccactgac cagccattct
    13261 cacatcgcgc tttgcgaaga tgccgccaac catatcgggc gcgtactgcg catggggccg
    13321 gggcaggcgt tgcaattgtt tgacggtagc aaccaggtct ttgacgccga aattaccagc
    13381 gccagcaaaa aaagcgtgga agtgaaggtg ctggaaggca agatcgacga tcgcgaatct
    13441 ccgctgcata ttcacctcgg tcaggtgatg tcgcgtggtg aaaaaatgga atttacgatc
    13501 cagaaatcga tcgaactcgg tgtaagcctc attacgccac ttttttctga gcgctgcggc
    13561 gttaaactgg atagtgaacg tctgaacaag aagctccagc agtggcagaa gattgcaatt
    13621 gctgcctgtg agcagtgtgg tcgtaaccgg gtgccggaaa tccgtccagc gatggatctg
    13681 gaagcctggt gtgcagagca ggatgaagga ctgaaactga atcttcaccc gcgcgccagt
    13741 aacagcatca atacgttgcc gttaccggtt gaacgcgtcc gcctgctgat tggcccggaa
    13801 ggcggtttat cggcagatga aattgccatg actgcccgct atcaatttac tgatatcctg
    13861 ttgggacctc gcgttttgcg tacagagaca actgcgctca ccgccattac cgcgctacaa
    13921 gtacgatttg gcgatttggg ctaacggaga agaataatga tcaagctcgg catcgtgatg
    13981 gaccccatcg caaacatcaa catcaagaaa gattccagtt tcgctatgtt gctggaagca
    14041 cagcgtcgtg gttacgaact tcactatatg gagatgggcg atctgtatct gatcaatggc
    14101 gaagcccgcg cccatacccg cacgctgaac gtgaagcaga actacgaaga gtggttttcg
    14161 ttcgtcggtg aacaggatct gccgctggcc gatctcgatg tgatcctgat gcgtaaagac
    14221 ccgccgtttg ataccgagtt tatctacgcg acctatattc tggaacgtgc cgaagagaaa
    14281 gggacgctga tcgttaacaa gccgcagagc ctgcgcgact gtaacgagaa actgtttacc
    14341 gcctggttct ctgacttaac gccagaaacg ctggttacgc gcaataaagc gcagctaaaa
    14401 gcgttctggg agaaacacag cgacatcatt cttaagccgc tggacggtat gggcggcgcg
    14461 tcgattttcc gcgtgaaaga aggcgatcca aacctcggcg tgattgccga aaccctgact
    14521 gagcatggca ctcgctactg catggcacaa aattacctgc cagccattaa agatggcgac
    14581 aaacgcgtgc tggtggtgga tggcgagccg gtaccgtact gcctggcgcg tattccgcag
    14641 gggggcgaaa cccgtggcaa tctggctgcc ggtggtcgcg gtgaacctcg tccgctgacg
    14701 gaaagtgact ggaaaatcgc ccgtcagatc gggccgacgc tgaaagaaaa agggctgatt
    14761 tttgttggtc tggatatcat cggcgaccgt ctgactgaaa ttaacgtcac cagcccaacc
    14821 tgtattcgtg agattgaagc agagtttccg gtgtcgatca ccggaatgtt aatggatgcc
    14881 atcgaagcac gtttacagca gcagtaaccc accttagcga gaaggatctc gttgagactc
    14941 tgagtgacag cgcccttctt tccacgcata ctgggcgctg ttgctttttt gaaccaggaa
    15001 acagaacctc tgacaatgaa tttacagcat cactttctta ttgccatgcc tgctctccag
    15061 gatccgattt tccgtcgttc cgtggtctac atttgcgaac ataataccaa tggtgcaatg
    15121 gggatcatcg tcaacaagcc gctggaaaat ctcaaaattg aagggattct ggaaaagctg
    15181 aagatcacgc cggagccgcg tgatgaatca atccgtctgg ataaaccggt tatgctcggc
    15241 ggtccgctgg ctgaagatcg cgggtttatt ttgcatactc cgccctccaa ttttgcttcc
    15301 agcattcgca tttcagacaa cacagtgatg accacttccc gcgatgtgct ggaaacgctc
    15361 ggcaccgata aacaaccgtc tgacgtattg gtggctctag gttatgcctc ctgggaaaaa
    15421 ggtcaactgg aacaagaaat tctcgataac gcgtggctaa cggccccggc ggatctgaat
    15481 attctgttca aaacgccgat tgccgaccgc tggcgcgagg cggcaaaact gattggtgtg
    15541 gatattctca ccatgcctgg tgtggcaggg cacgcctgat gagtggaacc ttactcgcct
    15601 tcgacttcgg caccaaaagc attggcgtag cggtcggcca acgcattacc ggcactgctc
    15661 gccctttgcc tgcaattaaa gcacaggacg gtacgccgga ctggaacctc atcgaacgtt
    15721 tactgaaaga gtggcagccg gacgaaatca tcgtcggttt gccgctgaat atggacggca
    15781 ccgagcaacc attgactgcc agagcgcgta aatttgccaa ccgtattcat ggtcgtttcg
    15841 gtgttgaagt aaagctccat gacgaacgtc ttagcaccgt ggaagcccgt tccggtctgt
    15901 ttgaacaggg cggctatcgg gcgctcaaca aaggcaaagt cgactctgcc tctgcggtta
    15961 ttattctcga aagctatttc gagcagggat attaaggcga tttaaaagcc tggcggatag
    16021 tcgttaccgc catcttgccg gatgcggcgt gaacgcctat ccggcctaca tgtggcagcg
    16081 attgtaggtc tgataagacg cgcaaacgtc tcatcagacg tttattgcct ttgtaggccc
    16141 gataagcttg cacatcgggc atggcaacgt cacaaacgcc cttcccccac tcgctgctga
    16201 aaactctgct gaaacgttat catccccacc tgctgcccgg tttgaataac atgcggtaac
    16261 tggtgggttt tcccttcgcg aatcaaattc cccaccgcgg gagtgttaat caataattca
    16321 aacagcgcca cgcgtccttc ctgtttatcc acttccagct tttgtgacaa cacggcccgt
    16381 aaactacctg ccagttgatt acgtacggga tctttttcct gcgccggaaa tgaatccacc
    16441 agtcgctcaa ctgcctgcgc ggcaccacgc gtatgtaatg ttgccagcac caaatgcccg
    16501 gtttctgccg ccgtcagtgc cagacggatt gtctcgctgt cacgcagctc tccgagcaaa
    16561 atcacatcag gatcttcccg caatgcggcc cgcaatcccg atgcgaacgt catacagtgc
    16621 aaaccaattt cccgcttctg gatcaaacat cgctggctgg catagatata ttcaacagga
    16681 tcttccagcg tcagaatatg cgcatcggca tgttgattga gatagccaac catcgccgcc
    16741 agcgtggtag atttgctgct ccccgtcgcc cccgtcacca gaatcaggcc attctcactc
    16801 ttgagtaatt ccggcaatac cgttggtgcg ccaagctgtt cgagctgcgg gcagtgcgaa
    16861 ggtaacagcc gtaacgccag cgaaatgcca tgccgttgcg caaatgcgct gccacgcaat
    16921 cgctggtttt ccgccagcga cacggcaaaa tccagctggc cattttccag caatattgcc
    16981 cgctgatcgt catccagcca ctcccgcagt agctcttcga cgtccggcgc ctcaaacggc
    17041 gcagcttcca ttagcccgcg aatgcgccat cgtgcgggcc aggcgctgca caggtgtaga
    17101 tccgagacgt tatgctttac actaagggcc acaatttctt ccatattcat actaagatcc
    17161 tcggaaaatg aacgatattg cgcataacct ggcacaggtc cgggacaaaa tctcagcggc
    17221 tgcaacgcgt tgcggccgtt ctccagaaga aattacgctg cttgcagtca gtaaaacaaa
    17281 acctgcgagc gccatcgcag aagccattga tgccgggcag cgtcaatttg gtgaaaacta
    17341 cgttcaggaa ggggtagata aaattcgcca ctttcaggaa ctgggcgtaa caggattaga
    17401 atggcatttt attggcccgt tgcagtctaa taaaagccgc ctggtggcag agcatttcga
    17461 ctggtgtcat accatcgacc gtttgcgcat tgctacccgc ctcaacgatc agcgcccggc
    17521 agaacttccc cctcttaacg tactgattca aattaatatt agtgatgaaa acagtaagtc
    17581 cgggattcaa ctggcagaac tggacgagct ggcagcggcg gtcgctgaac taccgcgttt
    17641 acgtctgcgc gggctgatgg caatccctgc gcctgagtca gaatatgtaa ggcagtttga
    17701 agttgcacgc caaatggctg tagcatttgc cggactgaaa acgcgctacc cgcatatcga
    17761 cacgctctct ctgggaatgt cggacgatat ggaagccgcc attgcggcag gtagcacgat
    17821 ggttcgtatc ggcactgcaa tttttggtgc gcgtgattac tctaaaaaat aaggaattaa
    17881 aggaacgcca tgaatacgtt gactttcctg ctttcaacgg tcattgagct gtataccatg
    17941 gtgctgttat tacgcatctg gatgcagtgg gctcattgtg atttttacaa ccccttctca
    18001 cagtttgtag tgaaggtaac gcagccaatt atcgggccac tgcgccgcgt tattccggca
    18061 atgggcccca ttgacagcgc ctcgctgctg gttgcctata ttctcagttt tatcaaagcc
    18121 atcgtgctgt ttaaagtggt gaccttcctg ccaatcatct ggattgctgg tttactgatt
    18181 ctgctgaaaa ccatcggcct gctgattttc tgggtcctgc tggtgatggc gattatgagc
    18241 tgggtaagcc agggtcgtag tccgattgaa tacgtgctga ttcagctggc cgatccgctg
    18301 ctgcgcccga ttcgccgcct gctaccggca atgggtggga ttgatttctc gccgatgatc
    18361 ctcgttctgc tgctgtatgt catcaatatg ggtgtcgcag aagtattaca ggcaaccgga
    18421 aacatgctgc tgccggggct gtggatggcg ttatgagtgc cgtaacagtt aatgatgacg
    18481 gtctggtttt acggctctat attcagccga aagccagccg tgattctatt gtcggtttac
    18541 atggcgacga agttaaagtc gccattaccg cgccgccggt tgacggccag ggcaacagtc
    18601 atctggtgaa gtttctcggt aagcaattcc gggttgccaa aagccaggtg gtgattgaaa
    18661 aaggcgaact tggccgccac aaacaaatta aaatcattaa tccgcaacaa atcccgccag
    18721 aaatcgcggc gttaattaat taggtatcct atgcaaaaag ttgtcctcgc aaccggcaat
    18781 gccggtaaag tgcgtgagct ggcgtcgctg cttagcgact tcggtcttga tatcgtggcc
    18841 caaacagacc tcggcgtaga ttccgctgaa gaaaccggcc tgacctttat cgaaaacgcg
    18901 attctgaaag cgcgctatgc ggcaaaagtg accggtttac cggcaattgc cgacgactct
    18961 ggtctggcgg tagatgcgct gggtggcgcg ccgggtattt actccgcgcg ttattccggt
    19021 gaagacgcga ccgatcaaaa gaatctgcaa aaactgttgg aaacactgaa agacgtaccg
    19081 gacgaccaac gtcaggcgcg tttccactgc gtgctggtat atctgcgtca cgcggaagat
    19141 ccgactccgc tggtatgcca cggcagctgg ccgggcgtga ttactcgcga accagcgggc
    19201 actggtggct ttggttatga tccaatcttc ttcgtacctt ccgaagggaa aaccgccgcc
    19261 gaactgaccc gcgaagaaaa gagcgccatt tcccaccgtg gtcaggcgtt gaaactgctg
    19321 ctggacgctt tacgtaatgg ttaaattacc gccgctgagt ctctacattc acatcccgtg
    19381 gtgcgtgcag aaatgcccgt actgcgattt caactctcac gcgttgaaag gagaagtgcc
    19441 gcacgacgat tatgttcagc atctgctttg cgatctggac aatgatgtgg cttacgctca
    19501 gggccgtgaa gtaaagacaa tctttattgg cggtggtacg ccgagcctgc tttccggccc
    19561 ggcgatgcaa acgctgctgg acggcgtgcg tgcgcgtttg ccgctggcag cggatgcaga
    19621 aattactatg gaagcgaacc ctggaacggt agaagccgat cgctttgtcg attatcagcg
    19681 tgctggtgtg aaccgcatct ctattggtgt gcagagtttt agcgaagaaa agctgaaacg
    19741 acttgggcgt attcatggcc cgcaagaagc gaaacgcgcg gcaaagctgg cgagcgggct
    19801 ggggctgcgt agctttaacc tcgatttgat gcatggacta ccggatcaat cactggaaga
    19861 ggcgcttggc gatctacgcc aggccattga actgaatccg ccgcatcttt cctggtatca
    19921 actgaccatc gaacccaata cgctgtttgg ttcgcgacca ccggtgctgc cggacgataa
    19981 cgcgctgtgg gatattttcg aacaggggca tcagttatta accgcagcgg gttatcagca
    20041 atatgaaact tccgcttacg ccaaacccgg ttatcagtgc cagcacaatc tcaactactg
    20101 gcgatttggt gactacatcg gtattggctg cggcgcgcac ggcaaagtca ccttcccgga
    20161 tgggcgcatt ctgcgtacca ctaaaacgcg tcatcctcgt ggttttatgc aaggaaggta
    20221 tctggaaagc cagcgtgatg tcgaagccgc agataagccg tttgagttct ttatgaatcg
    20281 cttccgtctg ctggaggccg cgccgcgcgt ggagtttatt gcgtataccg ggctttgcga
    20341 agatgtgatt cgcccacagt tagacgaggc gattgcccag ggttatctca ccgaatgtgc
    20401 ggattactgg cagataaccg agcacggtaa attattctta aactcacttc ttgaattatt
    20461 cctcgctgag taatcattaa tgcgcctgcg ggcgcattaa tcacttcttt tccccccgtt
    20521 aattaacttc ggcaaaatat atttcttact gaataaacat cgatgatatt tattagatat
    20581 atctagtgaa tatttactca tcatttattg attataaaca tgtacaacct ggcgcacaaa
    20641 tttaatattt aatttctgac tttatttcaa gttgtgataa acatagacaa gttgtcagac
    20701 aggttaacca atttccatcc ttcaacaggg agttttttta tgaaaatgag gtcatttacc
    20761 cgctcattag tctgcgcatc cttgctggct ctggttagca ctggcgtaaa cgccgcagaa
    20821 aaagtgacgt taaagttagc acacaacctg gaaagaagtc atgttgtaca tcagagtttt
    20881 gaagagctgg cgaaagaggt aaaacagctg tcagaaggta atatggtgat ccgtatttat
    20941 cccagcagcc agatgggtaa tgcgcgagaa actatggaat tgttgcaaaa tggcgcactg
    21001 gatatgacca aaggttccgc cagtgatctg gagtcttttg ataatattta tgctatctat
    21061 aatttgccgt tcttatttaa agatcaggct cacttcaaca aagttgtttt tggtgaagtt
    21121 ggtaaagaaa ttatggactc aacaaaagac aaaggctttt ttgctttatc agcttatgta
    21181 gcgggtacac gcagtttcta tgcgaaaaaa ccgattacta agccagaaga tcttaaaggg
    21241 ctgaaaattc gtgttcagcc aagtccgacc accatcaaaa tgattgaact gatgggtggt
    21301 tcacccactc caatttcatt tggcgaagtt tatactgcga tgcagcaagg tgtagtagat
    21361 ggcgcagaaa ataacgtgcc ttcctgggtg cagactcgac atattgaaat tgccaaagtt
    21421 ttttctgaag atgaacacgc ctcaattcct gactttttgg ttatctccac caaaacatgg
    21481 aataaattaa ccccagaaca gaaacaaatc ctcgaaacag cagcgaagaa atccgaagct
    21541 tatcaacaaa aattgtggga aaaaattgac gctgacacac gtgctcaggc gaaagctatg
    21601 gggggtgaaa ttgtaaaagt ggataaagca ccattccgtg ccgcagttca gcctctgttt
    21661 gacgacttca aaaaagatcc aaaacaagct gctttgttag agaagttcga caacgctgct
    21721 caataatatt ccgggccagt ctctggccct gattttccaa gatgcttaca atgatattta
    21781 accgcctgaa acttgcggta gaccgcgtca ttgccgcgtt ttctgtcgcc gtcatgcttg
    21841 ctctggttgt atgcgtagtc tggcaagtat ttagccgcta cgtacttaac cagccaagta
    21901 cattaaccga tgagctggca cgatttttga tgatctgggt agggttatta ggtgc
//
LOCUS       DAZPBM010000058        21749 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPBM010000058 DAZPBM010000000
VERSION     DAZPBM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.058"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            196..384
                     /gene="bcsR"
                     /locus_tag="ACHUZQ_002027"
     CDS             196..384
                     /gene="bcsR"
                     /locus_tag="ACHUZQ_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1025732.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHUZQ_002028"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHUZQ_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1025733.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="ACHUZQ_002029"
     CDS             1145..1309
                     /locus_tag="ACHUZQ_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR1025734.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHUZQ_002030"
                     /pseudo
     CDS             1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHUZQ_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHUZQ_002031"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHUZQ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1025735.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHUZQ_002032"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHUZQ_002032"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1025736.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHUZQ_002033"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHUZQ_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHUZQ_002034"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHUZQ_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1025737.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="ACHUZQ_002035"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="ACHUZQ_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1025738.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="ACHUZQ_002036"
     CDS             14459..15955
                     /locus_tag="ACHUZQ_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1025739.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHUZQ_002037"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHUZQ_002037"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1025740.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHUZQ_002038"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHUZQ_002038"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1025741.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="ACHUZQ_002039"
     CDS             18049..20109
                     /locus_tag="ACHUZQ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1025742.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="ACHUZQ_002040"
     CDS             complement(20291..21613)
                     /locus_tag="ACHUZQ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1025743.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
BASE COUNT         5035 a         5584 c         6129 g         5001 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctgaacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggcg acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttc
//
LOCUS       DAZPBM010000059        21712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPBM010000059 DAZPBM010000000
VERSION     DAZPBM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21712
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.059"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            54..1178
                     /locus_tag="ACHUZQ_002041"
     CDS             54..1178
                     /locus_tag="ACHUZQ_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1025744.1"
                     /translation="MFQKIKIRKMTISRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     IAERYMDDITTVDIANYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIARVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFKEKNQALYVIFHLALETAMRQGEI
                     LSLRWEHVDLQHGVAHLPTTKNGAPRDVPLSRKARNYLQMLPTQLNGNIFSYTSSGFK
                     SAWRTALQELKIENLHFHDLRHEAISRFFELGTLNVIEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDARRKQTSKIAPYFVPYPATVENRNGQVVVTLSDFDLETSAATKEQAIFH
                     ASVLLLRTLAQAAQRGERVPTPGELPTNIDERVMICPLTN"
     gene            1190..1843
                     /locus_tag="ACHUZQ_002042"
     CDS             1190..1843
                     /locus_tag="ACHUZQ_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025745.1"
                     /translation="MIEQLQSDFLFWIFIYFLIVGLLIRNIHLTRKIKSIEKNKMFPA
                     EEYAVLDIEQHMSNKYIGRLVTRFMKRTNGAPLLIVFPEYDKGDNNEDKNSPPFQLVK
                     LTSNINNIKEIYSNDDLNCFLNNISAQKNLYTTHSGPSQAWIEHAYPLQQVTILLQGT
                     RHSEREDIISQLKIILSRLKNGENTGFEHDDDFGYSFQYDAAAVGKSSFFDEAAGRK"
     gene            1873..2408
                     /locus_tag="ACHUZQ_002043"
                     /pseudo
     CDS             1873..2408
                     /locus_tag="ACHUZQ_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542050.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            2424..2828
                     /locus_tag="ACHUZQ_002044"
     CDS             2424..2828
                     /locus_tag="ACHUZQ_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1025746.1"
                     /translation="MALSPKEKKELQQRFEKQLLPDLDMSFELNGEVYKFNFDKNESG
                     DYIDSFTQAVWGGYQDAEEWEKYGKHQETELPECPEPVEGWCDCDKCKSMIFHKEKNI
                     YVCLSCHKQTEPFLPIDWNSNDYDWLPYEDEY"
     gene            2864..3112
                     /locus_tag="ACHUZQ_002045"
     CDS             2864..3112
                     /locus_tag="ACHUZQ_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025747.1"
                     /translation="MHSSFGLPYPAGHWFYSLQDLLDNPVFMVSFFVFWVATGQFLLT
                     TAHRKFNISETVEMVIIALLMILMTLSFYLCAILKASF"
     gene            3130..3459
                     /locus_tag="ACHUZQ_002046"
     CDS             3130..3459
                     /locus_tag="ACHUZQ_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025748.1"
                     /translation="MVTIIIGVSRVIDYKMGGVSVDIPPFPEWNSGHYIEIPGEGIVF
                     TGEKDDDGKRCYSGVIESHYSTDDWAFILSEYKNWLNDKERKFSYLFVLCNGELVYHE
                     VNKSELL"
     gene            3486..4073
                     /locus_tag="ACHUZQ_002047"
     CDS             3486..4073
                     /locus_tag="ACHUZQ_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6012 family protein"
                     /protein_id="HFR1025749.1"
                     /translation="MYLHIVPKLFHRMANKCTLKSISIPELDLIIDGESLSVGRPWPN
                     KCLWVGMRKSRKAVNGLLLHTDKKLRWFTTRYTWDIEDMGLIHHQVNTYIEDNEFDMV
                     SQEILLNGSFDKWSDRVHSAYEGKPPAIIQPKMESFLKRPGEDSHDMWEEFEWGNFLI
                     SREESILLHTIQSERLTTDFSLFKRQPSIESALVI"
     gene            4085..6241
                     /locus_tag="ACHUZQ_002048"
     CDS             4085..6241
                     /locus_tag="ACHUZQ_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFR1025750.1"
                     /translation="MKLFIAEKPAVANDIVKALGGNFTRHDGWFESDNTIVTNCFGHI
                     IESQPPENYNPEYKEWKIETLPLRLYPVKYQPVESAEKQVKTIIELIRRADVTEIIHA
                     GDPDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANPKNNRDFRGLYLKAL
                     ARSVADAIYGLSMTRAYTIPAKTKGYKGVLSVGRVQTPVLGLIVNRTRANKNHKSSFY
                     YTMTGHFQRGADVIRANWKPGEFAPLTDRKLLDKTWANGTATSLAGKPATVEAAATDD
                     KKTAAPLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQF
                     SEAPQIIDALKSVFDQPMDIDTTRKSKAFNSAKVTAHTAIIPTVSVPDVNALSTDERN
                     VYLAIAQHYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGVENAGDDE
                     AEVENDDSAFDLLCKIRTGETVTTKEVVVNEKKTTPPPLFTEATLLAALVRVADFVTD
                     PVIKKLLKDKDRDKKDEHGGIGTPATRASILETLKKRNYITLEKGKLIPTDTGYALID
                     ALPDIAVNPDMTALWAEKQTLIENGEMTIEQFVDELYNDLIPMISNANSAEIKVSPSA
                     PSGQSQRLSSPCPSCGKQIVIRPKSYSCTGCEFKIWNEFSGKKITQAQAEKLIKSGKT
                     DLIKGFKKKSGGTYDAVLVLEDKKTGKLGFPARAKK"
     gene            6716..7054
                     /locus_tag="ACHUZQ_002049"
     CDS             6716..7054
                     /locus_tag="ACHUZQ_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025751.1"
                     /translation="MKFIILKKDKDSTIARNDSVFPVNNFFVVLTTVSSVANSFGIVS
                     SLAKSIGISFPTGNVIETCFFGMFSLLLAAFSFVSTMGLANAVPPIKNVFPLLFFVLV
                     LITNYNIYFR"
     gene            7173..7379
                     /gene="hha"
                     /locus_tag="ACHUZQ_002050"
     CDS             7173..7379
                     /gene="hha"
                     /locus_tag="ACHUZQ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR1025752.1"
                     /translation="MKTKQDWLFQLRRCSTLDTLEKIIEKNQNCLPPSELESFNAAAD
                     HRLAELTMNKLYDKVPPSVWKYVY"
     gene            7525..7968
                     /locus_tag="ACHUZQ_002051"
     CDS             7525..7968
                     /locus_tag="ACHUZQ_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000465834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025753.1"
                     /translation="MFFLYETYNFFYYLIKLIVIQPQYICVYMIFFFFNAGIAYSITN
                     DIEDQVCRWLLFVSMLHALMIPPAVIMPLIMPPKEILQETEKRQELHESIPKTCKLKA
                     LDAQQGGLFGVDKDEWVFPDNKSFYLPEKCRPENRITELAMMKEG"
     gene            8213..8653
                     /locus_tag="ACHUZQ_002052"
     CDS             8213..8653
                     /locus_tag="ACHUZQ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025754.1"
                     /translation="MKEYEKTALIYSLKESNTLVLRIILISILVVGVISGAIYLYNLA
                     ISLFDSNPTSVQFTFCGVGCFIALLLQWISNVDANYRERIGEQPSTIKNIVQYLFAAI
                     ITISFITMALTAILVLKDSSNIWLNITFIALLLSIFVAIISVWG"
     gene            complement(8696..12907)
                     /locus_tag="ACHUZQ_002053"
     CDS             complement(8696..12907)
                     /locus_tag="ACHUZQ_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFR1025755.1"
                     /translation="MIVRYGGGNDGIVDYLINGRKAERQYTRDELDHRVVLDGDLQTT
                     DKIIDSIENKSQERYLHITLSFHESHVSNEVLKAIVDDYKKLLMNAYHPDEYSFYAEA
                     HLPKIRHIQDNSTGELVERKPHIHIVIPKVNLITEKFLNPVGDVTKGHTIEQLDAIQE
                     FINNKYNLDSPKDYPRKDADYGKIISRVKGDLYKEHHSELKGELLSRIENEKIENYSV
                     FKDIVAEYGELRIRNAGKTNEYLAVKLPGDKKYTNLKSPLFRQNYIETRTLTLEKPTH
                     KEIEKRLNTWLNKTSQEIKHIFNQAEKTRELYKTLSPLQQIDFLQERIKEYDSREKLN
                     ERNSQQTSGREGGYKSCPKKFARIRQSEATVGLSRMPQRGMVYGINGFTRPDSVSVLS
                     DISQRDLAEQLPQREHPGQDVRRDYDRQFTESGIKSLERSSFLCETMFQTLNEAAEKN
                     EITTMAEIRRNIDPVRFLSSAAERFNIIPAQHKIRTAKDGSPRFSVGNRNMNASDFLT
                     KHINLAWKDAKSFLLEVYSQQLENTPYTRYPTYRRLTYHEARERLNSLNLSEKTLRNT
                     IRFERGKLYNDLREMRRELKLIPREQRDIAVGVIVYKKLTTLERLSELDTEGRHIIRQ
                     YHADWHKDKDEMKALERLKSYLNFDEINAISADEPELSLQKAVDSQRRLEEAKKVNSK
                     LKDLVMDKQDSRIVYRDQESEKPVFTDKGNFVVAGKNPSKEEIGIMLEYSREKFGGVL
                     KLTGSEDFKKMCAEVAAEQDMKIILRPEQYQQMMLELKAELQGNKFEQVETQENSQES
                     ESRIEKGDALKEQATEQAQATEQTQATEQAQATEQTQATEQAQATEQAQATEQAQATS
                     SYDPGVITRANTLDSQMLSKGTNGEFGYLKSLDSDENEIWEVLGHVPGDSDDIFSVAS
                     FDNENDAKEFCNIVNELGIDRTQALIQEQLTTQHDQATAQVHNKQEIYCINFSRFHDL
                     NEGIVFHSKDAAIQCYEESKSSAIEKYESNYLNGDGFDTVVLMSKTVSTDELSSYPEG
                     ALVTFDRPFEIIANSYEEYRTPVYAVSFSKDEFSEDIKTFESLHDASEYKNKMLQEHG
                     LNQDDILITPVTREEIAFKGIKDAVNDANMAVMEQAGDSSRESPEEILASISANEHMI
                     SGLENFLVKDRSQFSSCNGDIVVEAEITRGEGGLYRLAVAGKHGLERGDAVARVDVTE
                     QQFAAITGKTPSEVLTGDQTSARVPVITGIHFSTRAIENVNKLEQQKDYVYFSTHEGL
                     NAEIKDFSSLKDAIEWGRVECELHDLNKRDTVIYRVESEHISQGIDAVMKNAERVERH
                     EIEKAQGRDCTPEDGKILEAIDRFEDKFRGEGLKFEREKAESDLLNHGFTREMAEDAL
                     GKQFVQAREDHQESQQQRDDSQDMH"
     gene            complement(12904..13245)
                     /gene="mobC"
                     /locus_tag="ACHUZQ_002054"
     CDS             complement(12904..13245)
                     /gene="mobC"
                     /locus_tag="ACHUZQ_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFR1025756.1"
                     /translation="MKKKTNKNVHVTFRLTEEEYAPFDRAIKELNISKSEFFRLLTIG
                     KINTYASDKRNIPEYKRCLSQLSWAGNNINQIAHRLNSDHLKGIISESLYKKVLNGLI
                     GIRDRLQEIAK"
     gene            13575..14054
                     /locus_tag="ACHUZQ_002055"
     CDS             13575..14054
                     /locus_tag="ACHUZQ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025757.1"
                     /translation="MNNGENKLLGSLLAQKVKRSKTGRIRERFAEIEEAQQQGIRNID
                     IVNALNDEGFDLTLKTFENILHRIRKERAEKKDVSHLLSNKEKTYQKAITIEDKNRKT
                     KQDNDILNAYLPVCFNNAKIAQQAIDNNVSIETIKSWNCANFVQVSNTLGNYIRNKR"
     gene            14060..14761
                     /gene="stbB"
                     /locus_tag="ACHUZQ_002056"
     CDS             14060..14761
                     /gene="stbB"
                     /locus_tag="ACHUZQ_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbB family protein"
                     /protein_id="HFR1025758.1"
                     /translation="MYLKLAIVNNSGNVGKSTIGQTLLKPRLEGSEIIKVETLNTDGT
                     NDEKLAAKEFDEIIKRIDDSECTIIDVGSSNIEQFLVQMNEYQGSHDLIDYFIVPVVR
                     EAKQQIDSIQTITTLMAMGVEQDRFKVIFNLAEKDTPINKQYSVFLADDVCKEIVGEN
                     PVVVYHNNIFNILNKSGLQYDDVYNDNRDFRSLIRSASSKEERQDLSNLRAVKMLMNG
                     FNSDLDVAFKNLNLV"
     gene            14808..15407
                     /locus_tag="ACHUZQ_002057"
     CDS             14808..15407
                     /locus_tag="ACHUZQ_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025759.1"
                     /translation="MSRSISEEFKDQKINDLLEDYIALEKQIEKTANLIINAIDETPA
                     RLDAVLSDKVNKIIQSSIDINKDINIAKSDIRQISNECQSEITKQAINTFSGIKQNFD
                     QIASDHKKQLEILTKSAKPFSIKTAIAICALCTLCLSAAFSGATWYVAQHEKEASLRF
                     YARAFLDMKKITEESMRKLPKSDQQNIKLKLDEIDSRKP"
     gene            15427..15549
                     /locus_tag="ACHUZQ_002058"
                     /pseudo
     CDS             15427..15549
                     /locus_tag="ACHUZQ_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15881..16144
                     /locus_tag="ACHUZQ_002059"
     CDS             15881..16144
                     /locus_tag="ACHUZQ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025760.1"
                     /translation="MATLTKKERAWLNELQDVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            16155..16475
                     /locus_tag="ACHUZQ_002060"
     CDS             16155..16475
                     /locus_tag="ACHUZQ_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025761.1"
                     /translation="MTTFTDKELIKEIKERIGSLDVRDNIERRAYEIALASLEAEAPN
                     LPGGFTIEEAKELHENLVKSHVSKALSGEKMKKENRDADLRWIHGVIVQAAWFVKASL
                     GQNA"
     gene            complement(16606..17043)
                     /locus_tag="ACHUZQ_002061"
     CDS             complement(16606..17043)
                     /locus_tag="ACHUZQ_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1025762.1"
                     /translation="MFFSVGVETPKDDHTAYGITVPVFDCFDFGCVSAADSQAEIPAM
                     AREAILAIVEEMVISGAHSVDDIHDEGCLTYSANPNYNHCDSWFVIDVDLSEIEGKQQ
                     RINISLPDVLIRRIDGYVRESGGVYKDRSHFLAQAARHELAYK"
     gene            complement(17068..17250)
                     /locus_tag="ACHUZQ_002062"
     CDS             complement(17068..17250)
                     /locus_tag="ACHUZQ_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR1025763.1"
                     /translation="MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKK
                     DLPLGTVRSIKKMAGI"
     gene            17547..17990
                     /locus_tag="ACHUZQ_002063"
     CDS             17547..17990
                     /locus_tag="ACHUZQ_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025764.1"
                     /translation="MTQLIPEKILEIIDDHDRAEKKQRNKIGFIYLCLCLAIIGVAAY
                     TFISTFSINVVTIIIMLFGVFMIVIFKSYLDSWSGIILSSDHILSILDKTKDYPEIKR
                     IVINRLLSGSILTGKDEEYIYSQLEKAQQSNEREKRLQAIKEYTS"
     gene            complement(18378..18656)
                     /locus_tag="ACHUZQ_002064"
     CDS             complement(18378..18656)
                     /locus_tag="ACHUZQ_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFR1025765.1"
                     /translation="MHFTTFLKKHFDIEKVVGTSDSGNDTESIYVYEKGNDCEPLFIL
                     HESWLNAEIKKCGVWTIGNIYSTLEHGKEYSEQELIKMIKEGKVISKY"
     gene            complement(19206..19457)
                     /locus_tag="ACHUZQ_002065"
     CDS             complement(19206..19457)
                     /locus_tag="ACHUZQ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1025766.1"
                     /translation="MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGT
                     ASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDSGSN"
     gene            complement(19447..19707)
                     /locus_tag="ACHUZQ_002066"
     CDS             complement(19447..19707)
                     /locus_tag="ACHUZQ_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025767.1"
                     /translation="MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTV
                     ADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMSEGKNAK"
     gene            complement(19759..19983)
                     /locus_tag="ACHUZQ_002067"
     CDS             complement(19759..19983)
                     /locus_tag="ACHUZQ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025768.1"
                     /translation="MKQSTFPVIVSTTGHVFSVVRVTLCTICLKHEKTGEAYVVIFTD
                     CHNIRDYKKGVVPVLGELYQEDVDLITGKS"
     gene            complement(20081..20290)
                     /locus_tag="ACHUZQ_002068"
     CDS             complement(20081..20290)
                     /locus_tag="ACHUZQ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025769.1"
                     /translation="MKVLIGNINIDNYHMLSALAGIAGFDRSIQFTCEISASIEIMED
                     DFVNKAGILKMLDEFIENDFSIKLV"
     gene            complement(20331..20654)
                     /locus_tag="ACHUZQ_002069"
     CDS             complement(20331..20654)
                     /locus_tag="ACHUZQ_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020314677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025770.1"
                     /translation="MENHAKFVATEILNQLGGNRFIAMTGAKNFACFDENGECGLCFR
                     LPSNFAMKGINLVKIKLTFSDTYLVTFSRVRGATVKEISKFDNIYCDQLECLFNEQTG
                     LATRL"
     gene            complement(21234..21563)
                     /locus_tag="ACHUZQ_002070"
     CDS             complement(21234..21563)
                     /locus_tag="ACHUZQ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025771.1"
                     /translation="MKTLTFNNGTVSVGDVFVSSWGYEQTNVNFYQVISVHGKKTVTV
                     QEIRASVHRTHSMSGYKTPLLNDFCGEPLKRRVRDYYSTPAIEIEEFETAYKGSPEEK
                     HEFTSYY"
BASE COUNT         6557 a         4584 c         4255 g         6316 t
ORIGIN      
        1 tgcatttcga agcaagaatc tctaccttcc cccctttttt gtctggaggg gatatgtttc
       61 agaaaatcaa aattcgcaaa atgacaatca gccgggcgct ggacaaatac ctgaagacag
      121 tatccatcca caaaaaaggc catctacagg agttttatcg cgtaaatgtg attaaacgtc
      181 accctattgc tgaacgctat atggatgaca ttaccactgt tgatattgcc aattatcggg
      241 atcaacggtt ggcgcaaatt aacccacgaa cggggcgtca aataaccgga aacaccgtcc
      301 gtcttgaact tgccctgttg tcatcattat tcaatatcgc ccgtgtggaa tggggaacat
      361 gccgcatgaa tcctgtcgaa ctggtcagaa aacccaaaat cagtagcggg cgcgatcgtc
      421 gcctgacttc aggagaagaa cgccgacttt cgcggtattt caaagaaaaa aatcaggctc
      481 tttacgtcat ttttcatctg gcgctggaaa cagcaatgcg acaaggtgaa atcttgtcat
      541 tgcgttggga acacgtcgat ctgcaacatg gggtagctca tcttccaaca accaaaaacg
      601 gtgctccacg cgatgttccg ctatccagga aagcccgtaa ttacctgcaa atgctgccga
      661 cacagcttaa cggtaatatt ttttcttata cctcttcggg attcaaaagt gcctggcgaa
      721 cagcacttca ggaactgaag atagaaaact tgcattttca tgacctccgt catgaggcta
      781 tcagccgatt tttcgaactt ggtacattga acgtcattga ggttgccgct atttcaggcc
      841 atcgctccct gaacatgttg aaacgataca cacaccttcg ggcttatcaa cttgtcagta
      901 aactcgatgc ccgacgcaag caaaccagca aaatcgctcc ctattttgtt ccctacccgg
      961 caacggtcga gaacagaaac gggcaagtcg ttgtgactct gagtgatttc gacctggaaa
     1021 caagcgcagc cacaaaagaa caggccatat ttcatgccag cgtgttgctg ctacgaacac
     1081 tggcacaagc cgcccagcga ggagaacgcg tccctactcc tggcgaactt cctacaaaca
     1141 ttgatgaacg ggtaatgatt tgtccattaa caaattagag gattcactta tgatagaaca
     1201 actacagagt gattttttat tctggatatt tatttacttc ctgatagtag gtttgcttat
     1261 cagaaacatt catcttacca gaaaaattaa atccatcgaa aaaaacaaaa tgttcccagc
     1321 agaggaatat gctgtcctgg acattgaaca gcatatgtcc aataaatata ttgggcggct
     1381 agtaacccgt tttatgaaga gaactaatgg tgcacctttg ttaattgtgt ttccagaata
     1441 cgataaagga gacaacaatg aagacaaaaa ttctccacca ttccaacttg taaaactaac
     1501 atccaacatc aacaatatta aagaaattta ttccaatgat gacttaaact gttttctgaa
     1561 taatatttcg gcacaaaaaa atttatatac aacgcactct ggacctagcc aggcatggat
     1621 tgaacatgca tatcctttgc aacaagtaac tattctctta caaggcactc gtcatagtga
     1681 aagggaagat ataatcagtc aacttaagat tattctttcc agactaaaaa atggagaaaa
     1741 tacaggtttt gagcatgacg atgattttgg atattcattt cagtatgatg ccgcagcagt
     1801 aggaaaatca tcatttttcg atgaagcggc aggaagaaaa taagaataac gctctatcca
     1861 ttgcacaggc aaattaacgt taatctttta tgaaaatctt attaacaata tttatttatc
     1921 tgcttaccgc ttttcagtct gcaaatgcag gtggaatacc ttatacattt aaccagaaaa
     1981 ttcttcaggg gaaagttatt cgcgttctcg atggcgacac catagaaatt aaaacactac
     2041 ccgcaaaaat tgttgtgtat gaagttccga tccgagtccg attgataaac atcgatgccc
     2101 ctgaaaagaa acaacctttt ggacgctggt caactagtca gcttaaaact ctggttgcag
     2161 gtaaacaggt gacagtctct tattcacata aagaccgcta cggacgcatt atcggtcatg
     2221 tgttcacgac gaacggcacg gatgccagcc gctttatggt tcagtctggc gctgcatggg
     2281 tatatgaacg ttacaacgtg gatgagtcat taccggctct gcaacgggaa gcgcaggaac
     2341 aaaaacgcgg tttgtgggcc gatgcaaatc cggttccacc gtgggaatgg cgatacaaac
     2401 ataactaact gatagaggtt tttatggctt tatcacccaa agaaaagaaa gagttacaac
     2461 aacgcttcga aaagcaatta ctacctgacc ttgatatgtc atttgaacta aatggcgaag
     2521 tatataaatt taattttgat aaaaatgagt caggtgacta tatcgatagt tttacacaag
     2581 cagtatgggg aggttatcag gatgccgagg aatgggaaaa atatggtaaa catcaggaga
     2641 cagaattacc tgaatgccct gaaccagtcg agggttggtg cgactgtgat aaatgtaagt
     2701 cgatgatttt tcataaagag aaaaatattt atgtttgcct ctcctgccat aaacaaacag
     2761 agccatttct tccaattgac tggaactcaa acgattacga ttggctacct tatgaagatg
     2821 aatactaatg ctgaatactt caaaatcata atgggggaaa gaaatgcatt cttcttttgg
     2881 tttgccatat cctgctggtc actggtttta ttctttacag gacttactcg acaatcctgt
     2941 atttatggtt tctttctttg ttttctgggt ggcaacgggt caatttttat taacaactgc
     3001 acacagaaaa tttaatatat ctgaaacagt tgagatggtt atcatcgctt tattaatgat
     3061 actaatgact ctttcttttt atctttgtgc aatattaaag gcttcttttt aaattagaga
     3121 ttaaaatcaa tggtgacgat aataattggt gtatcgagag ttattgatta taaaatgggg
     3181 ggagtgtcag ttgatattcc tccattccct gaatggaaca gtggtcatta tatagaaatt
     3241 ccaggagagg gtattgtatt cactggtgaa aaagatgatg acggcaaacg gtgttactct
     3301 ggtgtcatcg aatctcatta cagcaccgat gactgggcgt ttattctttc tgagtacaaa
     3361 aactggctga atgacaagga aagaaagttt tcttatctgt ttgtcttatg caatggtgaa
     3421 cttgtttacc atgaggtcaa caaatcagaa cttctttgac tacgataaag ataaaggaaa
     3481 tagatatgta cctacatatt gtccctaaac tttttcatcg catggcgaac aaatgcactc
     3541 taaaatccat ctccatacct gaacttgatt taataattga tggcgaatca ttgtctgtag
     3601 ggaggccgtg gcctaacaaa tgtttgtggg ttggaatgcg gaaaagcaga aaggcagtta
     3661 atggacttct tttgcataca gataaaaaac taagatggtt cactaccaga tatacctggg
     3721 atattgaaga tatgggatta attcatcatc aggttaatac ttatattgaa gataatgaat
     3781 ttgatatggt atcacaggaa attctcttaa atggttcttt tgataagtgg agtgacaggg
     3841 tgcattcagc ttatgaaggt aagcctccag caataatcca gcccaaaatg gaatcatttt
     3901 taaaaagacc aggtgaagac agtcatgata tgtgggagga gttcgagtgg ggtaacttcc
     3961 tcatatcaag agaagaaagc atcttgcttc acacgatcca gtctgaacga ttaaccaccg
     4021 atttctcttt attcaaaaga caaccatcta ttgaaagtgc attagttatt taaaggataa
     4081 taagatgaaa ctttttatcg cagaaaaacc cgcagtagca aatgatattg ttaaggcgct
     4141 tgggggcaat tttacccgtc atgatggctg gtttgaaagt gataatacta ttgtgactaa
     4201 ctgttttggt catattatcg aatcacagcc ccccgaaaac tataatcctg aatacaaaga
     4261 gtggaagatt gaaacactcc ctttgcgtct ttatcccgta aaataccagc ctgtagaaag
     4321 cgccgaaaaa caggttaaaa cgattattga gcttatcagg cgtgctgatg tgaccgaaat
     4381 tattcatgct ggcgatcctg atgacgaagg acagctactt gtcgatgagg tcctggaata
     4441 tgcaggcaac acaaaacccg ttaagcgcgt tctgatcaac gacaacaccc ttccggcagt
     4501 gaaaaaggcg ctggcaaatc ccaaaaataa ccgtgacttc agaggactct atctcaaggc
     4561 tctggcgcgt tctgtagccg acgcgatata cggtttatcc atgacgcgtg cttacacaat
     4621 cccggcaaaa actaaaggct ataaaggtgt tttgtctgtc ggacgggtcc agacacccgt
     4681 gcttggcctg attgtaaatc gcacccgtgc caataaaaat cataaatcca gcttctacta
     4741 caccatgacc ggacattttc aacgcggcgc tgatgttatc agggcgaact ggaaacctgg
     4801 tgagttcgca ccgctgacag accgcaaatt gcttgataaa acctgggcga atggaacagc
     4861 aacatcactt gcaggaaaac cagccacagt tgaagcggca gcaacagatg ataaaaaaac
     4921 ggctgcaccg ttgcccttta acctggtacg acttcagcag tacatgaaca aaaaattcaa
     4981 aatgactgct caaaaaacgc tggatatcac acagcagcta cgcgaaaaat acaaagcaat
     5041 tacttacaac cgttcagatt gttcatatct ttcagacgaa caattcagcg aagcaccaca
     5101 aataatcgat gccctgaagt cggtgtttga tcagccaatg gatattgata cgacacgtaa
     5161 aagcaaggca tttaacagcg caaaagtaac agcgcataca gccatcatcc caaccgttag
     5221 cgtaccggac gtaaacgcac tgagcaccga cgaacgcaac gtttacctgg caattgcaca
     5281 acactatctt gtccagttca tgcctgaaaa ggcatatcag gaagtatccg tcgccattca
     5341 gtgtggtgat gagtcgtttt atgcccgtgc caggaaaact acagacagcg gatttgaagc
     5401 gtttcttggt gtggaaaatg ccggggacga tgaagcagaa gtcgaaaatg atgattccgc
     5461 ttttgatctt ctctgtaaaa ttcgcacagg agaaacagtg acgacaaaag aagtggttgt
     5521 gaatgagaag aaaacaacgc caccgccatt attcaccgaa gccactttac ttgccgcgct
     5581 tgttcgtgtc gcagattttg tcactgatcc agtgattaaa aagttactga aggataaaga
     5641 tcgggataaa aaagacgaac atggcggtat tggtacgcca gctacgcggg cttccatatt
     5701 ggaaacactg aaaaaacgga actatatcac cctggaaaaa ggaaaactta ttccgacaga
     5761 taccgggtat gcgcttattg atgctctgcc ggatatagct gttaatccag atatgacggc
     5821 attatgggcg gaaaaacaga cactcattga aaacggagaa atgaccatcg aacagttcgt
     5881 tgacgaactg tacaatgatc tgataccgat gatcagtaat gcgaacagtg ctgaaataaa
     5941 agtttccccc agcgcgccgt ctggtcagtc acaacgcctg tcctctcctt gcccttcctg
     6001 tggtaaacag attgttatca ggccaaaaag ctattcctgt acaggatgtg aatttaaaat
     6061 ctggaatgag ttttctggca agaaaatcac ccaggcacag gccgaaaaac ttattaaatc
     6121 aggaaaaacc gatttgatta agggatttaa aaagaaaagc ggtggaacgt atgatgcggt
     6181 tcttgtcctt gaggataaga aaacagggaa gctgggtttt ccggcaaggg ctaagaagtg
     6241 aaagcaaagc agaaatggct tttcaggtca ggaattgctt cagttaatag ccaagtctgg
     6301 tcgtgggcga gcgccaaacc cgattgctga agcactggca ggaggtcgtt cccttgatga
     6361 gtttctcatc gagaaatgat tctacagccc tccttttctc tggagggcgt tatcctttga
     6421 aatatccgtt tattttgatg ataattctca tgtttcgttt ctcggcgtgg agagtacaga
     6481 ttttatggtg aatggtttgt tgcttttcgc tgttagtgta ttagtttttt tagtgataat
     6541 cctgatgtta catttgagaa cagccagtct tctttctgaa tgctctgaat atcgcacgaa
     6601 atgtgataat ctctatcatc tatgccagat gcaagagaaa gagttagaac atctacgtgc
     6661 actcattgat gcgcataaca tagattatta gacgttgctt tagggggatg gataaatgaa
     6721 atttattatt ttaaaaaagg ataaagatag cacaattgcc agaaacgact ctgttttccc
     6781 tgttaacaat ttctttgtcg ttttaactac cgtgtcttcc gtggctaact ctttcggaat
     6841 agtgtcttcc ctggctaaat ctatcggaat atcttttccc acgggcaatg ttattgagac
     6901 gtgttttttc ggtatgtttt cactattact ggctgctttc agttttgtaa gtacaatggg
     6961 gctggcgaac gcagtccccc ccattaaaaa tgtgtttccg cttttgtttt tcgttcttgt
     7021 cttaattact aattataata tttacttccg ataagaacgg gggcgcggca gcggcttttc
     7081 ttctgcccgt tctcccgtca ataaacccag cagtagaaat caaacatcgt tgtagctatt
     7141 atagggcaag gatgttcttc aggaattgta caatgaaaac aaaacaagac tggctctttc
     7201 agttaagacg ttgctcaaca ctcgacacac ttgaaaagat tattgaaaaa aaccaaaact
     7261 gcctcccccc ttctgagctt gagtcgttta atgccgccgc cgatcacagg cttgcggaac
     7321 tcacaatgaa taaactctac gataaagtcc ctccatccgt ctggaaatat gtatattagt
     7381 tttcatctat taatattaga tttttgatcg acacaaacat cagcaatccc tatacacctt
     7441 tcatagaatg aattgaaccc tgtatatagc ctgtctgaaa tggatatctg gcaggctatc
     7501 ttaatagtgt tataggtggt atgaatgttt ttcctctacg agacctacaa tttcttctac
     7561 tacctgataa agttaattgt cattcagcca caatatattt gtgtctatat gatattcttt
     7621 tttttcaatg cagggatagc atatagcatc accaatgata tcgaggatca agtatgtcga
     7681 tggcttttat tcgtaagtat gttacatgct ctgatgattc cccccgcagt tatcatgcca
     7741 cttatcatgc caccaaagga aatacttcag gaaacagaga aaaggcagga actacatgaa
     7801 tcaataccca aaacatgcaa attaaaggca ctggatgctc aacaaggtgg tttattcggc
     7861 gttgataaag atgaatgggt attccctgac aataaatcat tctatctgcc tgaaaagtgc
     7921 cgtccagaaa acaggattac ggaactagcc atgatgaaag aaggataaag atctatgaag
     7981 atattcaaat tgtttgttct ttgtatcccc cttctgttta gtcttttttt cgctggctac
     8041 ggcattttgt caaaaaatcc atttgaaaca ctaccgtgtc tgtccgccag tgctttttgc
     8101 ctttttctgt ttgacatact gaggcgatca tttatgaagt gaggtgtctt gatgttaacg
     8161 ttgaggttga gaaagttttg ttggttctta tacttataga ccggattaaa tcatgaaaga
     8221 atacgaaaaa acggctttaa tatattcctt aaaggaaagc aatacattag ttcttcggat
     8281 tatactaatc tctattttag ttgtcggggt gatttcaggt gcgatatact tatataactt
     8341 agcgatctcg ttgtttgatt caaacccaac gtctgtgcaa ttcacctttt gcggagtagg
     8401 ctgttttatt gctctattac ttcaatggat atctaatgtt gatgcaaatt acagagaaag
     8461 aattggggag caaccaagta caataaaaaa cattgtacaa tatttatttg cagccattat
     8521 taccattagt ttcattacaa tggcattaac agccatatta gtcttaaaag actcgtccaa
     8581 catatggctc aacattactt ttattgcatt attactgtcc atttttgtag ctattatttc
     8641 tgtttggggt tgactttttt tcttaaaaag cccgaaaacc gggctttttt cttaattagt
     8701 gcatgtcctg tgaatcgtca cgctgctgct gcgattcctg atgatcttct ctggcctgga
     8761 caaattgttt tccaagagca tcttctgcca tttcacgagt gaatccgtga ttaagcaaat
     8821 cggattcggc cttctccctt tcgaacttca gtccttcccc tctgaacttg tcctcaaagc
     8881 gatcaattgc ctcaagaatc ttgccgtctt ccggcgtgca atcccgtccc tgtgctttct
     8941 caatttcatg acgctccacg cgttcagcat ttttcattac agcgtcaatc ccctgagaaa
     9001 tatgttcaga ctcgacgcga taaatgacag tatcgcgctt attgagatca tgtagctcgc
     9061 attcaacccg gccccactca atagcatcct tgagagagct gaaatctttt atttcagcat
     9121 tcagaccttc atgtgttgag aaatagacat aatccttctg ttgttccagt ttattcacat
     9181 tttcaatagc ccttgtactg aaatggatac ctgtaattac cggaacacga gccgatgttt
     9241 ggtcccccgt taaaacttca gaaggtgttt ttccagtgat cgcggcaaat tgctgctccg
     9301 taacatccac tctggcaact gcatcaccac gctctaagcc atgcttacca gcgaccgcaa
     9361 ggcgataaag accaccttcc cccctggtaa tttccgcctc cacaacgata tccccattgc
     9421 aggaactgaa ctgagaacgg tctttaacca ggaagttttc aagcccggag atcatatgct
     9481 cgttagctga aatactggcg agtatctctt ctggtgattc tctggacgaa tcccccgcct
     9541 gttccatcac tgccatatta gcgtcattaa cagcgtcctt aattccttta aaggcgattt
     9601 cttctcttgt tacaggtgtg attaagatat catcctggtt aagcccgtgc tcctgaagca
     9661 tcttattttt atattcagat gcgtcgtgca atgactcaaa agtcttaata tcttcactaa
     9721 actcatcctt cgaaaaagaa accgcataca ctggggtacg gtattcttca tatgaattgg
     9781 caattatttc aaatggccta tcaaacgtta ctaatgcccc ctctggataa gaggataatt
     9841 catcggttga gacagtttta gacataagca caacagtatc aaaaccatca ccatttaaat
     9901 aattgctttc atatttttct attgcagaag atttgctttc ctcataacac tgaatagccg
     9961 catctttaga atgaaagacg ataccttcat tcaaatcatg gaagcgacta aagtttatac
    10021 aataaatttc ctgtttatta tgcacctgcg cagtagcctg atcgtgttga gtagtaagct
    10081 gctcctgaat cagagcctgt gttctgtcaa ttcctaattc attaacaata ttgcaaaatt
    10141 cttttgcatc gttttcatta tcaaaactgg caacactaaa gatatcatcg ctgtctccag
    10201 gtacatgccc cagcacttcc caaatctcat tttcatcgct atccagagat ttcaaatagc
    10261 caaattcacc atttgtgcct ttactaagca tctgtgaatc cagggtattt gccctggtaa
    10321 taacaccagg atcatatgaa gatgtggcct gtgcttgctc tgtggcctgt gcttgctctg
    10381 tggcctgcgc ctgctctgtg gcctgcgtct gctctgtggc ctgcgcctgc tctgtggcct
    10441 gagtctgttc tgtggcctgt gcctgttcgg tggcctgttc cttcaatgca tcacctttct
    10501 ctattctgga ttcggactcc tggctatttt cctgtgtttc cacctgctca aacttattac
    10561 cctgaagttc tgcttttaat tccagcatca tctgttgata ctgctccggg cgtaaaataa
    10621 tcttcatgtc ctgctctgcg gcaacttcag cgcacatttt tttgaaatct tcagagccgg
    10681 taagtttgag tacaccacca aacttttccc tggaatactc aagcattatt ccgatctctt
    10741 cttttgaggg atttttaccc gcaacgacaa agttcccttt gtcggtaaag acaggctttt
    10801 ccgattcctg atcgcgataa acaatcctgg aatcctgctt atccatcaca agatctttta
    10861 gtttggagtt aacctttttc gcctcttcta agcggcgctg ggaatccacc gctttctgaa
    10921 gcgaaagttc gggttcgtca gcagaaatgg cgttgatttc atcgaagttg agatagcttt
    10981 tgagacgttc aagggctttc atttcatctt tatccttatg ccagtcagca tgatattgac
    11041 gaataatgtg tcgcccctct gtgtcgagtt cagaaagacg ctcaagtgtg gttagttttt
    11101 tgtaaacaat cacacccacg gcaatatcac gctgttcacg tggtattaac ttcaactcgc
    11161 gtctcatctc tcgcagatca ttatacagct tgccacgctc aaacctgata gtatttcgta
    11221 atgttttttc cgataaattc agtgagttaa gacgctcacg ggcttcatga tacgtaaggc
    11281 gtctgtaggt tggatagcgc gtatatggcg tgttttctaa ctgctgtgaa taaacttcaa
    11341 gcagaaagga tttcgcatct ttccaggcaa gattaatatg ctttgtcaga aaatcagatg
    11401 cgttcatatt acgattcccc acagaaaacc ggggggaacc gtcctttgct gttctgattt
    11461 tatgctgcgc tggaataata ttaaaacgct cggcggcaga agacagaaac ctgacaggat
    11521 caatatttct gcgaatttct gccatcgttg taatttcgtt tttctctgcc gcctcattca
    11581 gagtctgaaa catcgtttca cacaaaaaag aagagcgttc aagacttttt attcctgact
    11641 ctgtaaattg tctatcataa tcccgtcgca catcctgacc aggatgttct ctttgcggta
    11701 attgctctgc cagatcacgc tgcgaaatat ctgacaatac actgacagag tcgggtcttg
    11761 tgaatccatt gattccgtaa accataccgc gttggggcat acgtgacaat ccgactgttg
    11821 cttcagattg acggattctg gcaaactttt taggacacga cttgtaacct ccctctcgtc
    11881 ctgacgtttg ctgagaattt ctttcgttaa gtttttctct tgagtcatat tcttttatcc
    11941 tctcttgcag gaaatcaatt tgttgtaaag ggcttaatgt cttgtaaagt tcccttgttt
    12001 tctccgcctg gttaaaaata tgcttaatct cttggcttgt tttatttaac caggtattaa
    12061 gtctcttttc tatctctttg tgtgttggtt tctcaagtgt taatgtcctt gtctcaatgt
    12121 aattctgacg aaaaagagga ctttttaagt ttgtgtattt tttatctccc ggcaatttta
    12181 cagccagata ctcattggtt ttaccagcat ttcttatacg aagttcacca tactctgcaa
    12241 caatatcttt gaatacagag taattttcaa ttttctcgtt ctctatacgc gaaagcaact
    12301 cacctttcaa ttcagagtga tgctctttgt aaagatctcc cttaactcta cttataattt
    12361 ttccgtaatc cgcatcttta cgcggatagt ctttagggct atcaagatta tatttattat
    12421 taataaattc ctgtatagca tcaagctgtt caattgtatg ccctttggta acatctccca
    12481 ctggattcag gaacttttca gtaatcaaat ttactttagg aattacaata tgaatatgtg
    12541 gttttctttc aacaagttct ccggtgctat tatcctggat atgacgaatt ttaggcaaat
    12601 gagcttccgc atagaaagag tattcatcag gatgataagc attcatcaat aactttttat
    12661 aatcatcaac aatagccttt aacacttcat tcgacacatg actttcatga aaagacagag
    12721 taatgtgcaa ataacgttcc tgacttttgt tttcaataga atcaataatc ttatctgtag
    12781 tctgtaaatc accatcaaga acgacacgat gatcaagttc atcacgcgtg tactggcgtt
    12841 ctgctttgcg accatttatc agataatcaa cgataccatc attaccacca ccgtatctga
    12901 caatcattta gctatctcct gaaggcgatc acgaataccg attagtccat ttaaaacctt
    12961 tttataaagc gattctgata taataccttt taaatgatct gaatttaatc ggtgcgctat
    13021 ttgatttatg ttgtttcctg cccaactcaa ctgagaaaga caacgtttgt attctgggat
    13081 gttacgttta tcagatgcat atgtgtttat tttaccaata gtaagcagcc tgaaaaattc
    13141 tgacttacta atattaagct cttttatagc cctatcgaac ggagcatatt cttcttcggt
    13201 aagtctgaat gttacatgaa cgtttttgtt ggtttttttc ttcatatgtc ctcaaggttc
    13261 gtagaaccgc ataagaacgg ctgcgtcgtt cggggtttca aaggggcatc cccctttggc
    13321 acacgattgt aacatgaccg gaacggtctt gtgtacaatc ggtatcgtgc ctgtgtttga
    13381 gaaaattatt tgattccata gcattcacac atcaaatgca caccataaac atatctaata
    13441 catagcaaaa acacaccata aaaaccaatg acatagcaca tacatagcaa acacatagca
    13501 tatacatagc aaaaattgct tgacgttcat gatagaattg ctcatcatga tttcactgtc
    13561 ctggagactg acaaatgaac aatggagaaa ataaactact tggttcgctc ttagctcaga
    13621 aggtcaagcg aagtaaaact ggaagaataa gagagcgttt tgctgaaata gaagaagcgc
    13681 agcaacaagg cataagaaac attgatattg ttaatgctct taatgatgaa ggttttgatc
    13741 taacgttaaa gacgtttgaa aacatattgc atcgcatacg aaaagaaagg gccgagaaaa
    13801 aagatgtttc tcatcttctt tcaaataaag agaaaacgta tcaaaaagca ataaccattg
    13861 aggacaaaaa tagaaaaacg aagcaagata atgatatact taatgcatat ttgccagttt
    13921 gcttcaacaa tgcaaagata gctcaacaag ctatagataa caatgtatct atagaaacaa
    13981 taaaatcttg gaattgtgct aactttgtac aagttagcaa tactcttgga aattatatac
    14041 gaaataaaag gtaagaaaaa tgtacttaaa actagcaatt gtaaataata gcggaaacgt
    14101 agggaaatcg accattggtc aaactttatt gaaacctcgt ctggaagggt cagaaattat
    14161 aaaagtagaa acattaaaca ctgatggtac aaatgacgag aaattagcag caaaggagtt
    14221 tgacgaaatt atcaaacgta ttgatgatag tgaatgcacc atcattgacg taggctcatc
    14281 aaatattgag caattccttg tacagatgaa tgaatatcag ggaagtcatg accttattga
    14341 ttatttcatc gttcctgttg tgcgagaagc aaaacaacaa atagactcaa ttcaaaccat
    14401 tacaacacta atggctatgg gtgttgaaca ggatcgtttt aaagtaattt tcaatcttgc
    14461 tgaaaaggac acccctatta acaaacaata ttcagttttc ttagctgacg acgtatgtaa
    14521 agaaattgta ggtgaaaatc ctgttgttgt ttaccataac aacattttca atattttaaa
    14581 taaaagcggc ctacagtatg atgatgtcta taatgacaat cgtgattttc gttcattaat
    14641 tcgttcagcg tcttcaaagg aagagcggca agatttgtca aatttaagag cagtaaaaat
    14701 gttgatgaat ggattcaaca gcgatcttga tgttgccttt aaaaacctca atctcgtctg
    14761 atacaatatg gccgggatgt acccggtcat aaaattggag aaaaacaatg tctcgtagta
    14821 tctctgaaga gtttaaagat caaaaaataa acgatcttct tgaagattat attgctttag
    14881 aaaaacagat cgaaaaaaca gcaaacttaa ttatcaacgc tatagacgag acacctgctc
    14941 gtctggatgc cgttctgtca gacaaagtaa ataaaataat acaaagcagc attgatatta
    15001 ataaagatat taatattgca aaaagcgata taagacaaat atctaatgag tgtcagagcg
    15061 agataaccaa acaagcaata aatacttttt ctggaatcaa acaaaatttt gatcaaattg
    15121 cttccgacca taaaaaacaa ctagagatac taactaaatc agcaaaacca ttcagtatca
    15181 aaacagccat tgcaatttgt gccctgtgca cattatgcct gtctgcggca ttttctggcg
    15241 caacctggta tgttgcacaa catgaaaaag aggcaagtct gcgcttctat gcaagagcat
    15301 ttcttgatat gaaaaaaata actgaagaga gcatgagaaa attaccaaaa tcagaccagc
    15361 aaaatataaa actcaaacta gacgaaattg attcaagaaa accataaata tgaaaaggag
    15421 tccctaatga ccacttttac cgacgaggac aaagaactaa ttaaagaaat cagagagcga
    15481 attggcagcc tggacgtgcg agacaatatt gagcgtcggg cttatgaaat tgctctggca
    15541 tcgctggaag cagaggcagt tatgttctgt atatcaggac aaaatgtaga ttcagaagaa
    15601 catgtatcaa ccagcaaagc ggttgttgat gcctgggttg aagaatggaa tcaggttgac
    15661 ggaagtcctg gcgaaccact gtacaaaact atgccactct actatcacgc tgccttgcca
    15721 gcgccggtag tgcctgaagg actggttaaa gcggtgcgtt tttatgagca ggtaaaaaat
    15781 gagaatccgg cagtcgaaac cgtagcatgg aaagacgctg ttgactgggt tctcgaagag
    15841 gcttgtcagg ctgtaaacat tggcatcaaa ggagagtgat atggcaactt taacaaaaaa
    15901 agaacgggca tggttgaacg aattacagga cgttcttgat cgctgcccat caccgaaaaa
    15961 aattggtttt tacaccattg gcgataaaag catttacctg tatgacctgc gccgcatgga
    16021 tgaaatcatg gaggctcttg ataatcgttc gtcgatggat tggtgtgttg ctgtccatga
    16081 tatgaatgcc ggatttgatg aaaagatttt attcccctca tcagttgaaa gcacagcagg
    16141 ataaggacta acacatgaca acgttcaccg acaaagaact gattaaagaa atcaaagagc
    16201 gtataggcag cctggacgtc cgagacaata ttgagcgtcg ggcttatgaa attgctctgg
    16261 catcactgga agcagaagcg ccaaatttgc ctggtggttt cacaattgaa gaagcaaaag
    16321 aattacacga aaatctggta aaaagtcacg taagcaaggc attaagtggc gaaaaaatga
    16381 aaaaagaaaa tcgtgatgcc gatttacgct ggattcatgg cgtaatagtt caggctgcgt
    16441 ggtttgtgaa agcatcacta gggcagaacg catagtgcgt acctgctttt ctcctggcaa
    16501 cggtggatct tccgcttcct cgtcgctcgc tcgctatgct cggtcgctgg actgcggcga
    16561 gcggttagct atcaaaaaaa atccccgcac aacgcgggga tagttttatt tatatgcaag
    16621 ttcatgacgt gccgcctgag ccagaaaatg gctacgatct ttgtatacgc caccactttc
    16681 tctcacatat ccatcaatac gacgaatcaa tacatcaggc aatgaaatat taatacgttg
    16741 ttgtttgccc tcaatttctg acaaatcaac atcaattaca aaccagctat cacagtggtt
    16801 ataatttggg ttggcggaat aagtcaaaca cccttcatca tgaatatcat caacagaatg
    16861 agcaccgctt attaccatct cttccacgat tgccaatatc gcttcacgcg ccattgcggg
    16921 tatttctgcc tgagaatcag cagcagaaac acatccaaaa tcaaagcaat caaaaaccgg
    16981 aacggtgata ccgtatgctg tatgatcatc tttcggtgtt tcgacaccaa cagaaaagaa
    17041 catggctacc tccaaagtcg gcggggatta aatccccgcc atttttttga tcgatctgac
    17101 agtacctagc ggcaagtcct ttttaggatg aggaacagga aaagtttttc ctgttattgg
    17161 cgaccaccat atctggtgac tgcttgcctt atgccgtttc aactcgcaac cagcagctat
    17221 cagttctttc aacaggtcag cagatttcat atgtcctccc taacctgagg ataattatac
    17281 acacacatac acacaaaggc aaattatttc attcgtatga cttatgaacc acactgtaaa
    17341 gacagctcaa tctttacagt gtaagtcatt tatccagttt tatcgctgtg tatgtgatac
    17401 atagaaaata atatagtatt ttactatatg atatctgatt gacactttta actgcacatc
    17461 tcatcattta catcgtaaag acaaacatag atttacaatg taaagctatg taattaaaca
    17521 ctccgtgtcc caaataagga aagaaaatga ctcaactaat cccagaaaag atactggaaa
    17581 tcatagatga tcatgatcgt gcagaaaaaa agcaacgcaa caaaatcggt ttcatttatt
    17641 tatgtctctg tttagcaata attggtgtgg ccgcgtatac ctttatatca acgtttagca
    17701 ttaatgtggt aacaatcatc ataatgcttt tcggtgtgtt tatgattgtg atttttaaga
    17761 gctatttaga ctcatggagt gggataattc ttagcagtga ccacatattg tccatactcg
    17821 ataaaaccaa agattaccca gaaattaaac gtatagttat taatcgtctg ctctccggct
    17881 caatactcac gggtaaagat gaagagtaca tatacagcca actggaaaag gcccaacaaa
    17941 gcaacgaacg tgaaaagaga ttgcaagcaa ttaaagagta tacatcgtaa tacaacgcat
    18001 tacgtcgaaa taccctggat catcgaagcc acatacccct aagcgcagcg caaccgccgg
    18061 aaaacgcacc acactgtaaa gacaataaaa tctttacaac gtaaagctaa ctatgtaata
    18121 caacgcgcta cacaataaga aagggagagc atgacactct ccccatatat gactcaaact
    18181 aaagcattat cccggttatg cgcccaccaa atgatttcgc taataatcca tgcttctatc
    18241 gcgggtgttt ttacaggttc cccatttaca atgcggtttg caagctctat catttcacat
    18301 actgacattt gagaatctcc aaagatgcat ctgtcatttg gtaacaggct gccccacagc
    18361 ctgttaccaa ccccaaatta atatttgctg attactttac cctccttaat catttttatt
    18421 agttcctgtt cagaatattc ttttccgtgt tccaatgtgg aatatatatt gccaatagtc
    18481 cataccccac actttttaat ttctgcattt agccaggatt catgcagaat aaacaacggt
    18541 tcgcaatcat ttcctttttc ataaacataa atagattcgg tatcatttcc agaatcagaa
    18601 gtaccaacga ctttttctat atcgaaatgt ttttttagaa aagttgtgaa atgcattttc
    18661 atgctcctgc cgctgagcgg ctttggtggt tggggaccat cccctcccga tgtgtttata
    18721 ataggtcaga actgacctat tatcaagtat ttttccctcc attcttcaaa aaaaacacca
    18781 tcaaatacag acagttacat ccatgtgatg cccgacaggg catggaaggg gcgaaagaaa
    18841 ataccaggga gcagcggggc cacatcgccc cgaagcgcgg cgcagccgct gacgaacata
    18901 ccaggatgca cagggccatc ccttcgccct gcaaagccag aacagaccaa caggccatga
    18961 tcatgctgat ttgcgccgca gaatgccgca gttagcgcga gcgacagcga gtttacgccg
    19021 tcctggtgca ttgcagcagg gcggggcgct gtactgcgcg tgtgagcgtc agcgagctac
    19081 gggcgcaatt gctcattgtt caatgcaact ggggctatta agtccagcca taaattcagt
    19141 tatggttttt gtagcttatt ccgtgatttc gtcatacgtt aattctattt tcatcttaat
    19201 attccttaat tacttccaga atcagaattc tttttattcc ttttacaaag tacataatca
    19261 gggtgttctc cagctaaaag cagaagaaat ttaaactcta ctggtgtaag tgctgtaccg
    19321 cttgcagttc cgacaggttc ttctttgcga cgccaggtac gcggactcat gctgaatatt
    19381 tcggcacatt ctttctgtgt caggccaatt ttttgccgca gacgtttaat gttttctggt
    19441 gtgttttcat ttggcatttt ttccctcact catttcctga atcatcatag aaacaatttc
    19501 tttatcattc tctcttaatc cgtcgctggc aaagtcgata gtgtcgaatg ccttctcatg
    19561 aaaaacaaga tcggcaacag ttttcaccgt tgctttcgtt tttcctaaag tcgcagactt
    19621 agaaaaagtt tttactcgct tcaccagatg aaacgtatca ccttctttat tcagataata
    19681 ttgtctgtca ccttttctta tcttcatatc aagatagtct ccccttttaa cagtaaaggg
    19741 gcatatagcc cctttctttt aactttttcc ggtaatcaaa tcgacatctt cctgataaag
    19801 ctccccaaga accggaacaa cgcccttttt ataatcacgg atattatggc agtctgtaaa
    19861 aatgacgaca taagcctcgc ctgttttttc atgtttaagg caaattgtgc aaagcgtcac
    19921 gcgcacaaca gaaaacacat gaccagttgt agagacgata acaggaaaag tagattgttt
    19981 catatttaat ctcctgccgc tgagcggctt tggcgattgg ggaccatccc ctctcaatgc
    20041 attaataata ggtcataaat gacctattat caagtatttt ctaaacgagc tttattgaaa
    20101 aatcattttc aataaattca tctaacatct ttaatatacc agctttattt acgaaatcat
    20161 cctccataat ttcgatactt gctgatattt cacatgtaaa ctgaatgctc ctgtcaaatc
    20221 ccgcaatccc agctaatgca gacagcatat gataattatc aatatttata ttaccaataa
    20281 gaactttcat ttatcctcct gatatggggg gaatcccccc atattaaaaa ttacaatctg
    20341 gttgcaagtc cggtttgttc attaaataaa cattcaagct gatcacaata aatattatca
    20401 aattttgata tttcttttac tgtagctcca cggacacggg aaaatgtcac caggtatgta
    20461 tcagagaagg ttaatttgat tttaaccaaa ttaattccct tcattgcaaa atttgaaggc
    20521 aaacgaaagc aaagaccaca ctcaccattt tcatcaaaac aggcaaagtt ctttgcgcct
    20581 gtcatagcaa taaagcggtt tccaccaagc tgattaagaa tttcagtagc aacaaatttg
    20641 gcgtggtttt ccatttttaa gctcctgccg ctgagcggct ttggtgattg gggaccatcc
    20701 cctctcgatg tgtttataat aggtcagaac tgacctatta tcaagtgttt tttcctcctt
    20761 tctttaaaat aaataccatc aaatacagat ggttagattc atgtaatgcc cgacagggca
    20821 tagaaggggc gaaagaaaat accagggagc agcgggacca catcgccccg aagcgcggcg
    20881 cagccgctga cgaacatacc aggatgcaca gggccatccc ttcgccctgc aaagcccaac
    20941 ggactgacag gccatgatca tgctgatttg cgccgcagaa tgccgcagtt agcgcgagcg
    21001 ccagcgagtt tacgccgtcc tggtgcattg cagcagggcg gggcgctgta ctgcgcgtgt
    21061 gagcgtcagc gagctacggg cgcaacatta cattgctgca ttcgcctggc attctccacg
    21121 ccttgaagga cggagtttta ctgcgcacca aataaaaaag aggcgattaa gcccctgtat
    21181 tcactattaa caatttaaca tggttgcagg ggataccccc tgcaactatt ttcttagtag
    21241 tatgatgtga actcgtgttt ttcttccggt gatcctttat atgctgtttc gaattcctca
    21301 atttcaattg ctggcgtact gtaataatca cgcactcgcc gttttaatgg ctcaccacag
    21361 aaatcattaa gtaatggggt cttatatccg ctcattgaat gagtccgatg aactgaagcg
    21421 cgaatctcct gaacagttac ggtttttttg ccatgaacag aaataacctg gtagaaatta
    21481 acgttcgttt gctcatatcc ccaggaagat acaaacacat cgccaacaga aacagtgccg
    21541 ttattaaaag ttaatgtttt catttttatc tcctgccgct gagcggcttt ggtggttggg
    21601 gaccatcccc tcccgatgtg tttataatag gtcagaactg acctattatc aagtattttt
    21661 ccctccattc ttcaaaaaga ataccatcaa atacatatag ttatattcat tt
//
LOCUS       DAZPBM010000060        21643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPBM010000060 DAZPBM010000000
VERSION     DAZPBM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.060"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHUZQ_002071"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="ACHUZQ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1025772.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="ACHUZQ_002072"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="ACHUZQ_002072"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1025773.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHUZQ_002073"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="ACHUZQ_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1025774.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="ACHUZQ_002074"
     CDS             complement(3717..4511)
                     /locus_tag="ACHUZQ_002074"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1025775.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="ACHUZQ_002075"
     CDS             4649..4990
                     /locus_tag="ACHUZQ_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1025776.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHUZQ_002076"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="ACHUZQ_002076"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1025777.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="ACHUZQ_002077"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="ACHUZQ_002077"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1025778.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="ACHUZQ_002078"
     CDS             8906..10198
                     /locus_tag="ACHUZQ_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1025779.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10229..10326
                     /locus_tag="ACHUZQ_002079"
                     /pseudo
     CDS             10229..10326
                     /locus_tag="ACHUZQ_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="ACHUZQ_002080"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="ACHUZQ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1025780.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="ACHUZQ_002081"
     CDS             complement(10731..11189)
                     /locus_tag="ACHUZQ_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1025781.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="ACHUZQ_002082"
     CDS             complement(11186..12103)
                     /locus_tag="ACHUZQ_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1025782.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="ACHUZQ_002083"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="ACHUZQ_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1025783.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="ACHUZQ_002084"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="ACHUZQ_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1025784.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHUZQ_002085"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="ACHUZQ_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1025785.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHUZQ_002086"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="ACHUZQ_002086"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1025786.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHUZQ_002087"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="ACHUZQ_002087"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR1025787.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHUZQ_002088"
     CDS             16063..16749
                     /gene="ybbA"
                     /locus_tag="ACHUZQ_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1025788.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHUZQ_002089"
     CDS             16746..19160
                     /gene="ybbP"
                     /locus_tag="ACHUZQ_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR1025789.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHUZQ_002090"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="ACHUZQ_002090"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1025790.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHUZQ_002091"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="ACHUZQ_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5685 c         5799 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgacctggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccagtg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg acggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttc aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgttg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctaggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAZPBM010000061        21455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPBM010000061 DAZPBM010000000
VERSION     DAZPBM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.061"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(331..876)
                     /gene="hemG"
                     /locus_tag="ACHUZQ_002092"
     CDS             complement(331..876)
                     /gene="hemG"
                     /locus_tag="ACHUZQ_002092"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1025791.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(888..2339)
                     /gene="trkH"
                     /locus_tag="ACHUZQ_002093"
     CDS             complement(888..2339)
                     /gene="trkH"
                     /locus_tag="ACHUZQ_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1025792.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2378..2992)
                     /locus_tag="ACHUZQ_002094"
     CDS             complement(2378..2992)
                     /locus_tag="ACHUZQ_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1025793.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLMTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="ACHUZQ_002095"
     CDS             complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="ACHUZQ_002095"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1025794.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4513..6702
                     /gene="fadB"
                     /locus_tag="ACHUZQ_002096"
     CDS             4513..6702
                     /gene="fadB"
                     /locus_tag="ACHUZQ_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1025795.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6712..7875
                     /gene="fadA"
                     /locus_tag="ACHUZQ_002097"
     CDS             6712..7875
                     /gene="fadA"
                     /locus_tag="ACHUZQ_002097"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1025796.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8065..8766)
                     /gene="fre"
                     /locus_tag="ACHUZQ_002098"
     CDS             complement(8065..8766)
                     /gene="fre"
                     /locus_tag="ACHUZQ_002098"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1025797.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="ACHUZQ_002099"
     CDS             complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="ACHUZQ_002099"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1025798.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10473..10961
                     /gene="rfaH"
                     /locus_tag="ACHUZQ_002100"
     CDS             10473..10961
                     /gene="rfaH"
                     /locus_tag="ACHUZQ_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1025799.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="ACHUZQ_002101"
     CDS             complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="ACHUZQ_002101"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1025800.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="ACHUZQ_002102"
     CDS             complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="ACHUZQ_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1025801.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="ACHUZQ_002103"
     CDS             complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="ACHUZQ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1025802.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="ACHUZQ_002104"
     CDS             complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="ACHUZQ_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1025803.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="ACHUZQ_002105"
     CDS             complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="ACHUZQ_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1025804.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="ACHUZQ_002106"
     CDS             complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="ACHUZQ_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1025805.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="ACHUZQ_002107"
     CDS             complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="ACHUZQ_002107"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1025806.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="ACHUZQ_002108"
     CDS             complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="ACHUZQ_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1025807.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18102..18863)
                     /gene="udp"
                     /locus_tag="ACHUZQ_002109"
     CDS             complement(18102..18863)
                     /gene="udp"
                     /locus_tag="ACHUZQ_002109"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1025808.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19125..19937
                     /locus_tag="ACHUZQ_002110"
     CDS             19125..19937
                     /locus_tag="ACHUZQ_002110"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1025809.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20331..21014
                     /gene="tssD"
                     /locus_tag="ACHUZQ_002111"
                     /pseudo
     CDS             20331..21014
                     /gene="tssD"
                     /locus_tag="ACHUZQ_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            20994..>21455
                     /locus_tag="ACHUZQ_002112"
     CDS             20994..>21455
                     /locus_tag="ACHUZQ_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025810.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT         5266 a         5741 c         5373 g         5075 t
ORIGIN      
        1 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
       61 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcact ttttctcagc
      121 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      181 tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg
      241 tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt
      301 ttctgaccgc gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg
      361 ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg
      421 cgtatccgtt tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca
      481 gcgataacgt gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg
      541 cgagttcatc agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt
      601 gcgcgccacc aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg
      661 ttttttgaca aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat
      721 gaccacacgg tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc
      781 cgtctggatc cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg
      841 tccgtccctt gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag
      901 aaagtcgggg taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg
      961 gcaatcagga tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca
     1021 agccctggcc ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc
     1081 acgcccgtgg cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa
     1141 aatccccaaa cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta
     1201 tagacggcgt tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc
     1261 aggatgcgga tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct
     1321 gaacataaaa gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac
     1381 ccggcggttg tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc
     1441 gaactgtaga cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt
     1501 acgccgataa acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt
     1561 aacagtgaaa agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata
     1621 gtgttaatag tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca
     1681 atagcgatag tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac
     1741 aacgccagcg cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt
     1801 tccgcaattc gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag
     1861 agctgcatcc cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc
     1921 atcccgccaa accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc
     1981 agccccacca gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca
     2041 tcggtaatcg tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc
     2101 agcacggtcc agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc
     2161 tctttacggt tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg
     2221 gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg
     2281 gtccctgaaa ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc
     2341 tctgccgctt ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt
     2401 gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg
     2461 gaagcgccac ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac
     2521 actgccccag caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt
     2581 cggttaatgg cgtcttgcgt tgggtcatta gctggcgcag cgcctgattc acgccgccgc
     2641 catacgcttt cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca
     2701 cagcggtaat ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg
     2761 ttcctgccgg ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg
     2821 ccacccacgc cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa
     2881 acgctttcgc cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt
     2941 tgatctcttc aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag
     3001 tttcagatcc cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat
     3061 acgaatgccg ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa
     3121 ctgcccttca cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt
     3181 taacaccata cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc
     3241 cgcgaggtgt gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgcaggcc
     3301 cagcggatga ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc
     3361 ttcttcactc atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg
     3421 gaactggatg tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag
     3481 ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca
     3541 ggtacgagtc aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct
     3601 gcgcatctct tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc
     3661 gttaagcgcc acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag
     3721 ataggcgatg ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg
     3781 atgaccgttg accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt
     3841 gaaggagcgg tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc
     3901 aatacccagt tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc
     3961 agcgggcagc aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc
     4021 agtccagaag gaggtcggca gcggttcgac gttgtgccag taatcaaccg gcagatagaa
     4081 ccacagtttc ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac
     4141 cggcacccac gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa
     4201 aacgttgaac agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc
     4261 atcgcgagtc cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc
     4321 cattttttac ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc
     4381 cagagcgtga tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat
     4441 atttttaaca caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt
     4501 caggagactg acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc
     4561 attgccgaac tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc
     4621 gccagcctcg gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg
     4681 ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg
     4741 ttcctcgttc ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat
     4801 cgcctggaag atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt
     4861 ggctgcgaat gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt
     4921 ctgccggaaa ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt
     4981 atgctgggcg ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat
     5041 caggcgctga aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc
     5101 gcaaaggcgg ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag
     5161 ccgaagctgg aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct
     5221 aaaggaatgg tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa
     5281 accattgaag ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt
     5341 tttgtcccgc tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat
     5401 caatatgtaa aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct
     5461 gctgtgctgg gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc
     5521 gtgccggttg tcatgaaaga tatcaacgac aagtcgttaa ccctcagcat gaccgaagcc
     5581 gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc
     5641 gtgatctcca cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg
     5701 gtagaagcgg ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa
     5761 aaagtacgcc aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg
     5821 gccaacgcgc tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac
     5881 cgaatgccgt tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa
     5941 gttgtcgcct gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc
     6001 ttctttgtta accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac
     6061 ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc
     6121 ccggcatatc tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg
     6181 gcagcaggct tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt
     6241 gatgccaacc gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc
     6301 aaaggtaagc cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc
     6361 cagccgaagc gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc
     6421 aacgaagtgg tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg
     6481 gcgctggtct acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac
     6541 accctcggta gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg
     6601 tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg
     6661 gttgagccag cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag
     6721 gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt
     6781 aacgtgcgtg cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg
     6841 gcgctggaag cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag
     6901 cagggtttta atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg
     6961 gcggttaccg tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga
     7021 atgatcatga ctggcgatgc gcaggcatgt ctggttggcg gcgtggagca tatgggccat
     7081 gtgccgatga gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg
     7141 gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa
     7201 atgcaggatg cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc
     7261 gcatttaaaa atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag
     7321 tttaattacg acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt
     7381 ccggcgtttg atccagtaag cggtacggta acggcgggta catcttctgc actttctgat
     7441 ggtgccgcgg ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg
     7501 cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc
     7561 ccggttcctg cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc
     7621 gtgtttgaga tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg
     7681 ctaatggagc agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat
     7741 ccgctgggtt gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa
     7801 gacgttcagt ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg
     7861 tttgagcggg tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc
     7921 ctgcaccaat gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt
     7981 gccggatgcg acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc
     8041 ctgtcagggg cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc
     8101 ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg
     8161 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa
     8221 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     8281 cagaccagga tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc
     8341 ttcacgccca ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa
     8401 taaaatcgag cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc
     8461 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     8521 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     8581 ctcgataaac cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc
     8641 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat
     8701 tcggacacgg tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     8761 tgtcatgcgc tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc
     8821 ttttaccgtt gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat
     8881 caggatcttt tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg
     8941 tggcatccag ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat
     9001 caataggcgt attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg
     9061 cccaaatcac atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt
     9121 tagtgtacat aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc
     9181 cggcgtactg ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca
     9241 ggtaaaaatc gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca
     9301 gtgcgacacc cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa
     9361 tcgcatcttc acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt
     9421 tatagtaacc ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat
     9481 acccttccag cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca
     9541 ccacttcggt cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg
     9601 gaacgggggt gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg
     9661 ggaaacgttc gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat
     9721 gcgacagcca gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca
     9781 gattctgccg ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg
     9841 cggcatcttc cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag
     9901 agacgatttt ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt
     9961 gcttaaactg cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt
    10021 tcagaaacgc caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc
    10081 ccatcgccac gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt
    10141 tagggttttc gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag
    10201 tgatttccag atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa
    10261 gcagcgtcaa gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat
    10321 ctacgcccat tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa
    10381 ctctgacggt ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc
    10441 cttgcgcccc gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactgca
    10501 agcgcgggca acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg
    10561 caccgatgat caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat
    10621 tgtttcccaa ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca
    10681 acgcgacccg cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat
    10741 cggcggtgat tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc
    10801 cttatccggg agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt
    10861 tcaccgaacc cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga
    10921 ttaagcacag tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg
    10981 ttttgacatt agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg
    11041 caatacgttg caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg
    11101 gcgtgagatc gcgagggagc agatacggcg catcagtttc gatcagtaat ttttccgccg
    11161 gaatcaacgg caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg
    11221 taatgccgat ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa
    11281 agcaatgaag aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc
    11341 gctcgtgggc atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta
    11401 gctgggcaac aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga
    11461 gaccacattc accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt
    11521 cagtcgcagc ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg
    11581 acgaatactg acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga
    11641 tgagtagccc attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg
    11701 caaattgcga actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac
    11761 cgccctgacg ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt
    11821 ttcctcttcc cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc
    11881 aaacagacag tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag
    11941 caacatcccg acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc
    12001 tggcgaggta atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac
    12061 accaaacgcc ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac
    12121 ctgcacccct tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa
    12181 gtaggcgaat gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac
    12241 caggcgacgt tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat
    12301 caccggcgct gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg
    12361 cgaggccacg tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc
    12421 ggataccagg tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac
    12481 cgcgataatg cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg
    12541 agtatcttct acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt
    12601 ttcggttcag cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt
    12661 tccggcgaac tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca
    12721 ttatctttca ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg
    12781 tttgcaacgt aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct
    12841 ttcagttcgg gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga
    12901 aactcctgga gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc
    12961 aacgcgcgaa tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga
    13021 acgacgaggc cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat
    13081 tacacctgct ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta
    13141 tccgcctgct tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc
    13201 tgctttggtt catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg
    13261 gaaccgatgg agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc
    13321 aataactgcc aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa
    13381 caaggccgca ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg
    13441 cgccaaccga caaaccaagc gatcagacca cctgccatta accagccggg catcagcccc
    13501 cattcaggtc ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg
    13561 agaaaataac gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta
    13621 tcaacactgt gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt
    13681 tctggcattt tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga
    13741 ataccgacct gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg
    13801 agctgcggat aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac
    13861 accagttgcg gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt
    13921 acatgtccaa acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga
    13981 atggcaaatt cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt
    14041 agctctgcca ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag
    14101 cgtttatctt ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg
    14161 ttttccgggt gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag
    14221 ctgtcgcgaa agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg
    14281 ttggtgccat ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc
    14341 atcaccatca tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg
    14401 ctgtcttcaa aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt
    14461 tcatcaatca aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca
    14521 tccggcagca aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta
    14581 ataaccggca aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc
    14641 aatcgcgcgg tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg
    14701 tcaaaccacg cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc
    14761 ttgccatcaa acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt
    14821 ggaaaaagat cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt
    14881 tcttgcaggg ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc
    14941 cagaataatg agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt
    15001 tcatcaagtc cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct
    15061 ggcgtcattt agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga
    15121 cggcagccgt ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact
    15181 cttcagtaat ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact
    15241 ttgcgcctcc gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg
    15301 ccagcagttc cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca
    15361 cctgaatatc gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc
    15421 gatcgcgaag tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg
    15481 cccattcgcc cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa
    15541 agccttttac ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg
    15601 cgggtgagcg atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt
    15661 taaaaggcat ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct
    15721 gccgtcagat tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg
    15781 gtatcctgat cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg
    15841 ttcgcgacca gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg
    15901 ctcagcggct cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc
    15961 agcacgcgat acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac
    16021 gaaatggtga tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga
    16081 acatactcaa cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc
    16141 atggattcat tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat
    16201 ttcgctgtca ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg
    16261 ccgctgcaat caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca
    16321 ttcatgacat cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt
    16381 tgttccttcg cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc
    16441 tcaatgcctg ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct
    16501 agatttctac ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc
    16561 atcgcgttga taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc
    16621 attctggctc accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc
    16681 taaaccgcga aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt
    16741 cattgcctgg cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc
    16801 atcgatgaac aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc
    16861 gttgcggctt tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag
    16921 cgtagtcggg ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg
    16981 gcggtcaagc gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag
    17041 agttcgcagc cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg
    17101 caccgacgcg atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa
    17161 atagcgttca taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg
    17221 cagccgcacg atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg
    17281 ggtttcatat tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac
    17341 ctcgccccag ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc
    17401 ttcctgggcc atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg
    17461 gcgttcttgt gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg
    17521 ttcacgtagc ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg
    17581 attgctgtgc tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc
    17641 gctgttaatc atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt
    17701 ggttacttca cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc
    17761 gttattgagt aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc
    17821 cgcgcttaac tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc
    17881 atgttgataa ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta
    17941 aaccatgatt gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga
    18001 attgtgctgt acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata
    18061 caaaaggccg aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc
    18121 gcttccacca cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg
    18181 atctcttgct gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga
    18241 cttgcacaca tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc
    18301 tgccactctt ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa
    18361 cgttcctgac ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg
    18421 ccaatggatt tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg
    18481 aattccagcg gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc
    18541 aggacatcac ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag
    18601 gtgcgaatgc ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata
    18661 ccggtagagc agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg
    18721 cggtgagatg ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc
    18781 gggtcgccag ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga
    18841 tgaaaaacat cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag
    18901 caaaaagaca ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca
    18961 aatgcgttgc ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt
    19021 ctgcaggact gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta
    19081 tagttaacat tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac
    19141 aatctggatt tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg
    19201 acgcgattgt ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc
    19261 atgccagacc aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt
    19321 ttggcgtgca tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg
    19381 ctatcgcacc tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca
    19441 cgttgcttag cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc
    19501 atgccgccag ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat
    19561 tctgctgggg tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg
    19621 cagtggcgtg gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg
    19681 ttgatatcgc aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca
    19741 gcattccgca ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag
    19801 cagagattat cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct
    19861 atcatgccga atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg
    19921 gagagaagcg gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg
    19981 ttttgctgcc atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac
    20041 tccgctattt gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg
    20101 aagacacctt agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa
    20161 cacattcaca ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa
    20221 acattttgtt tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac
    20281 aacaatacta tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata
    20341 ttatttacct gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga
    20401 gcgcatcaat aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc
    20461 tctctaatag tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta
    20521 aattgattga caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt
    20581 tcatggagtt tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca
    20641 ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg
    20701 atacagaagc catcactgtc agttattaat atatactctc caggcatctg attgctaata
    20761 ctgaatttag ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa
    20821 aaactaaagc tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg
    20881 ctgctgtcgg catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg
    20941 ggggagatgc acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg
    21001 gtgcttcaat agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac
    21061 ttgagcattt cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg
    21121 gtggtccaat agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta
    21181 ttgttgaaaa acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata
    21241 gactgaaaga cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata
    21301 ctcatgagtg tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg
    21361 atggtgacgc aggctatgca ctctggaatc atactggatt tgcccctata tttccagaca
    21421 tctgttatca cttaacccat tacaagcccg ctgcc
//
LOCUS       DAZPBM010000062        21258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPBM010000062 DAZPBM010000000
VERSION     DAZPBM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.062"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUZQ_002113"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHUZQ_002113"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1025811.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUZQ_002114"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHUZQ_002114"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1025812.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUZQ_002115"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHUZQ_002115"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1025813.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUZQ_002116"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHUZQ_002116"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR1025814.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUZQ_002117"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHUZQ_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR1025815.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUZQ_002118"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHUZQ_002118"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1025816.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUZQ_002119"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHUZQ_002119"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1025817.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHUZQ_002120"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHUZQ_002120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1025818.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUZQ_002121"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHUZQ_002121"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1025819.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHUZQ_002122"
     CDS             complement(12799..14139)
                     /locus_tag="ACHUZQ_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1025820.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUZQ_002123"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHUZQ_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1025821.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHUZQ_002124"
     CDS             complement(15928..16377)
                     /locus_tag="ACHUZQ_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1025822.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUZQ_002125"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHUZQ_002125"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1025823.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHUZQ_002126"
     CDS             complement(17177..17506)
                     /locus_tag="ACHUZQ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1025824.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHUZQ_002127"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHUZQ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1025825.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACHUZQ_002128"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACHUZQ_002128"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1025826.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHUZQ_002129"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHUZQ_002129"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1025827.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5189 a         5703 c         5066 g         5300 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cctggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 gcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc gcaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaggcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgcgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg aaacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatg
//
LOCUS       DAZPBM010000063        21003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPBM010000063 DAZPBM010000000
VERSION     DAZPBM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.063"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUZQ_002130"
     CDS             complement(<1..170)
                     /locus_tag="ACHUZQ_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR1025828.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUZQ_002131"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUZQ_002131"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1025829.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHUZQ_002132"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHUZQ_002132"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1025830.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUZQ_002133"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUZQ_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1025831.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUZQ_002134"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUZQ_002134"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1025832.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUZQ_002135"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUZQ_002135"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1025833.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHUZQ_002136"
     CDS             4288..5151
                     /locus_tag="ACHUZQ_002136"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1025834.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="ACHUZQ_002137"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="ACHUZQ_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1025835.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="ACHUZQ_002138"
     CDS             6489..7106
                     /locus_tag="ACHUZQ_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1025836.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHUZQ_002139"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHUZQ_002139"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1025837.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLAHKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="ACHUZQ_002140"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="ACHUZQ_002140"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1025838.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHUZQ_002141"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHUZQ_002141"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1025839.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="ACHUZQ_002142"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="ACHUZQ_002142"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1025840.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="ACHUZQ_002143"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="ACHUZQ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1025841.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="ACHUZQ_002144"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="ACHUZQ_002144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1025842.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHUZQ_002145"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHUZQ_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1025843.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="ACHUZQ_002146"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="ACHUZQ_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1025844.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="ACHUZQ_002147"
     CDS             18072..19781
                     /locus_tag="ACHUZQ_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1025845.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHUZQ_002148"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHUZQ_002148"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1025846.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5716 c         5385 g         4819 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggct
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttatgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacttgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaactt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggca tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcacc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAZPBM010000064        20839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPBM010000064 DAZPBM010000000
VERSION     DAZPBM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.064"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHUZQ_002149"
     CDS             complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHUZQ_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1025847.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2096..2962
                     /gene="yghU"
                     /locus_tag="ACHUZQ_002150"
     CDS             2096..2962
                     /gene="yghU"
                     /locus_tag="ACHUZQ_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1025848.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2991..3072)
                     /locus_tag="ACHUZQ_002151"
                     /pseudo
     CDS             complement(2991..3072)
                     /locus_tag="ACHUZQ_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHUZQ_002152"
     CDS             complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHUZQ_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1025849.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHUZQ_002153"
     CDS             complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHUZQ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1025850.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHUZQ_002154"
     CDS             complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHUZQ_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1025851.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4161..4655)
                     /locus_tag="ACHUZQ_002155"
     CDS             complement(4161..4655)
                     /locus_tag="ACHUZQ_002155"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1025852.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHUZQ_002156"
     CDS             complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHUZQ_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1025853.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHUZQ_002157"
     CDS             complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHUZQ_002157"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1025854.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHUZQ_002158"
     CDS             complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHUZQ_002158"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1025855.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHUZQ_002159"
     CDS             complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHUZQ_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1025856.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHUZQ_002160"
     CDS             complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHUZQ_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1025857.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHUZQ_002161"
     CDS             complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHUZQ_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1025858.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11269..12309
                     /gene="gpr"
                     /locus_tag="ACHUZQ_002162"
     CDS             11269..12309
                     /gene="gpr"
                     /locus_tag="ACHUZQ_002162"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1025859.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12349..12843)
                     /locus_tag="ACHUZQ_002163"
     CDS             complement(12349..12843)
                     /locus_tag="ACHUZQ_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1025860.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13034..13918
                     /gene="yghA"
                     /locus_tag="ACHUZQ_002164"
     CDS             13034..13918
                     /gene="yghA"
                     /locus_tag="ACHUZQ_002164"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1025861.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14078..14503)
                     /gene="exbD"
                     /locus_tag="ACHUZQ_002165"
     CDS             complement(14078..14503)
                     /gene="exbD"
                     /locus_tag="ACHUZQ_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1025862.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14510..15244)
                     /gene="exbB"
                     /locus_tag="ACHUZQ_002166"
     CDS             complement(14510..15244)
                     /gene="exbB"
                     /locus_tag="ACHUZQ_002166"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1025863.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15496..16683
                     /gene="metC"
                     /locus_tag="ACHUZQ_002167"
     CDS             15496..16683
                     /gene="metC"
                     /locus_tag="ACHUZQ_002167"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1025864.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16823..17482
                     /gene="yghB"
                     /locus_tag="ACHUZQ_002168"
     CDS             16823..17482
                     /gene="yghB"
                     /locus_tag="ACHUZQ_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1025865.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17522..18421)
                     /gene="yqhC"
                     /locus_tag="ACHUZQ_002169"
     CDS             complement(17522..18421)
                     /gene="yqhC"
                     /locus_tag="ACHUZQ_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1025866.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18615..19778
                     /gene="yqhD"
                     /locus_tag="ACHUZQ_002170"
     CDS             18615..19778
                     /gene="yqhD"
                     /locus_tag="ACHUZQ_002170"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1025867.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19883..20710
                     /gene="dkgA"
                     /locus_tag="ACHUZQ_002171"
     CDS             19883..20710
                     /gene="dkgA"
                     /locus_tag="ACHUZQ_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1025868.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
BASE COUNT         5127 a         5610 c         5229 g         4873 t
ORIGIN      
        1 cataatcagt acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa
       61 tatcactctc ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt
      121 tgccgcccac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
      181 acagttgctg atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct
      241 cttcatgatg gctgaccagg tcgatattgc taccacagcg accggcaatc ggtttcactg
      301 cgtaacctgt tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat
      361 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc
      421 acagcggctc aaagactagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt
      481 tttgcggatg accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa
      541 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt
      601 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccaatcca cgcaagatac
      661 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
      721 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
      781 cagccaattc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
      841 ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt
      901 actcgtaaac cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat
      961 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc gggatgtcga
     1021 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcaggt
     1081 gcagctcgtt ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat
     1141 gataaggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgcagcggat
     1201 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccaggcgc gctccgctga
     1261 ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
     1321 ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttcagggta tagcagccgt
     1381 tttcgaccac catctccagc tcacgcgtcc actgttgccc ttgcggcaac ggggaatgaa
     1441 tcacgttctg ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat
     1501 ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
     1561 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc
     1621 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt
     1681 aattcagaaa gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt
     1741 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
     1801 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
     1861 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
     1921 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
     1981 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
     2041 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
     2101 agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt
     2161 cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa
     2221 acacccattg caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
     2281 tgaggagctg ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat
     2341 tggcgatggc gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc
     2401 ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct
     2461 gctttatctg gcggagaaat ttggctactt cctgccgcag gatttggcaa agcgtactga
     2521 aacgatgaac tggctgttct ggttacaggg cgcggcaccg ttcctcggcg gtggttttgg
     2581 tcacttttac cattacgcgc cgatgaaaat tgagtacgcc atcaaccgct ttaccatgga
     2641 agccaaacgt ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg
     2701 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaatg tggtgttagg
     2761 cggcgtgtat gatgccgcag agtttcttga tgcgggcagt tataagcatg tacaacgctg
     2821 ggcgaaagaa gtaggcgaac gtccggcggt gaaacgtggg cgtattgtta accgcaccaa
     2881 cggaccgctg aatgagcagt tgcatgagcg ccatgacgcc agtgatttcg agacgaatac
     2941 ggaagataag cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat
     3001 aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct
     3061 tatcaggcct acgaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3121 tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3181 taccagcacc cactggccca gtagatcggc agggttacct tcacaaatca gggcgatatt
     3241 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3301 agccagcacc tggcctggaa cgccaataca cattattaac tccggttatt caacttcaat
     3361 acttttgacg atcagcgaat cgccggtatc gacacgcaac cgctcgccgt gacagagcgg
     3421 acactgcgca tcgtgctgat gaatctccac cacctggctg caatcccagc accaagcctg
     3481 ggcgggttta tagacgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat
     3541 ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcgccaa tttccagcca
     3601 cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgttggataa tttcaacggc
     3661 gctctggcaa agagacaact catgcatttt cgccactcct gcgaccaaac agcagggcgc
     3721 gacgccctgc gtgtggtaca tccggattcg tgactggcag cgaaaggatc attcgtgcgc
     3781 agtcatccgt caggcgttgg ccctcttcaa tcgacatgct gtgcgaaagc ggcgacatca
     3841 gcgaacagga gagatattgc gaaacaccgt ccagttcgcc aacggtaaag gtcatagtgc
     3901 catacggcag ttgcagacca attttttcac tcactttgcg cagcggccag agttgatccg
     3961 ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac
     4021 cttcgaacag cgtaaaatca gaaacataca ccggcattga aggatgcaga aaagagagat
     4081 cgtgcatcga acgccgggca atttcttcaa acgctgcctg tacttgcgcc ttcggggagg
     4141 tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcct
     4201 gattcgcgca gcgcagccag aacctgctca agcgcaggtt caatcattgc ttcaaccgtc
     4261 ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca cgccgaccag ggtcagcttt
     4321 ttcggaaact caccggtgaa gcgcagggcc gacaatacgt cggccaggcc aagctgatgc
     4381 ggagagattt tgttggtaaa caacgccgga acttcttcat cccgcaggat catcatcgtt
     4441 cccggcgtgt tctttttcga cacaatggca tccgcaataa tcagatgatc gcgatttgcc
     4501 atgtcgccaa gcagctccat tcccgccgtg ccgccatcga ggatctcaac ataatccggc
     4561 agaatgtatc gttgctctaa cgcttcgaca atccgcacac cgatggcttc atcggtcagc
     4621 aaaatattgc cgacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt
     4681 cgttgccgtc ggcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa
     4741 tggtacgcac cacttccagc ggtttattcg gatcagcaac cggtgtaccc accagcgact
     4801 gctcgtaagg accgacgtca tcattgaagt tacgcggacc agagttccag gttgatggaa
     4861 caactgcctg gtagttactg ataataccgt ctttaataac catccagtga gagagcatac
     4921 cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc cgttgccgga atgttcggtt
     4981 tcacaaaggt ggtgtgatcg cctttgccga tattggtgat cagtgcactg tattgatttt
     5041 gcaggatatc ctgcaattcg cagcagtgaa cggtacgacc aataatacgg cccagcgtgg
     5101 agtgcagctg cgccacttcc agcgtgttgc cagtcagttt ctgataaatc gcaacgattt
     5161 cattcagttt gttctgggta gattcgcgac ctgccgccag tttcaccagc atattagcca
     5221 gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttcc
     5281 cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggt gcctgcggcg
     5341 cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat gcctttgatc aggtattcat
     5401 cggaatgaga agtgatcgga cgatacgagg acagatccgc attctcaatg tagccgcccg
     5461 ggaacaggaa gctgccgttt ttactgtcgg tcgggaattc cggcacgctc aggtagttca
     5521 ccgcaccttt accgcgcgtc agccattccg ggtagaacgc ggcaataact gcggtatcaa
     5581 ccttataaac ctgttcaaca aagtcgctca gcttgtcgat gaaagacttg atgtacatca
     5641 ggcgctcaag gttcagcacg cccaaaccgt cgaggttgat cgggttcgcg acaccaccta
     5701 ccgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt
     5761 cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc acttccggcg
     5821 gcagtttcat cgccgggtga cccccgtagc cattggcgaa aatacccaac tgaccgctgg
     5881 caaccagatc tttgatcttg ttctgaactt tagtgaactc ttccggactg ttcagatgcc
     5941 aggtcgaaac gcctttcagc atttcggagg ctttggttgg gtcagcttgc agtgcagaag
     6001 tgatgtccac ccagtccagc gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg
     6061 tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca
     6121 gcgcactttc tgccgcacga acggaagaca gcgcgtgagt ggtagtacat acgccacaga
     6181 tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc
     6241 cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat
     6301 cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc
     6361 tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag
     6421 acgaatgagt acgatgtaag cgcaaatctc aatagccaca aaaccaatag aaatcaacag
     6481 ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata
     6541 ggtcagacgc caggttgcac aacctaacag tgcgctcagt gctgacagga acagcatgcg
     6601 ggaatcatta cgcagcttcg ccacacgcag aacgaccagc gggaagagca tcagcaggac
     6661 ttcaatccag aacatcacgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg
     6721 atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt
     6781 ggtcagcttg acgaacagac tcttttcatc cggaccgttg ccacgcagac ccgcctgcac
     6841 cagcgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa
     6901 cagcggcaac atttcatagc tctgccacaa cggatgcacc ttgtagcccg ccgagatcat
     6961 cagtgacccc attgaagact ggtgcatggt cggcaacagc gcgccaagtg caatgatgaa
     7021 gaacataacc ttgttcagac gcttgagaga aaccttccag cccagacgtt caaacagtgc
     7081 cggagcaaac tccagtgcca tcacgccgat atagatggtc atacagaccg ccgtctcgaa
     7141 cagtaccgag ttcacgttga agtgacccgg aatgtagaag tacggcaggt tccagtagcg
     7201 acccacgtcg atagtgatcg acaagccacc cagtgagtaa ccaaacagac tcgccaacag
     7261 cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg cccacgccag
     7321 cgcccagccg ccacaggcaa agccggtgcc aatcagcagg tcaaacgcga tccacacgcc
     7381 ccacgggaag ccgccgttca ggtcagagac agagcccaga ccgaacacca gacgcttcac
     7441 aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat
     7501 tttgccgccc agcggttgtg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt
     7561 ttttggtgtt gcgacgaacc agcacggtta agcccgccag cacagccagt ggtagcatca
     7621 tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca
     7681 gtttcggcag gtcgagattt tcataaggca cacccgtcag taccagaacc tgagtaccgc
     7741 cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt
     7801 cgccagattt cagcgtctga cgcggatagt ggtattcgct gccaggcttc agcgccagac
     7861 gttttttcgc ctccgccatc agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc
     7921 acacttctac gcagccaggc agaccgcctt tatcgagacg ttccacacct ttctggttgc
     7981 acagctcgca cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt
     8041 acggacaggc gaccatgcag taacggcaac cggtgcacac gtctttgtcg taatggacaa
     8101 tgccggtttt cggatctttt ttcagtgcag agaccgggca cacagagaca cagttcggat
     8161 cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt
     8221 tgacccctgt gccgctggtc cacacctgaa tgatgttatt ggtatacggc gacagtttgt
     8281 cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaagttg atatcctgac
     8341 acttggtgac gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac atccccagcg
     8401 atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg
     8461 tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc
     8521 ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacgct
     8581 gacaccggca accagcccaa ccacaccgcc gagcaaacca atagcgcctg cagagacgtt
     8641 gccgccctct ttagcgttaa catccggttt ctgtgaacgc ggagtctgat tttcgacgtt
     8701 ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc catagcatgg
     8761 gtgaccaatc gctaccggcc acacgccgcc aacatcgcag aattgcagcg ttgagcagtt
     8821 gccgtaagtt tctggccctt tacagccgag gtggtacagg caccagcctt cacggtggcc
     8881 ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg
     8941 ttcgtgaatc agacggccat aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg
     9001 tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca
     9061 gcccggaata ttgataacgg ttttgcctgg cagaacttct tgcaggctga ctgcgccagt
     9121 tgggttaact ccagctgcgg caacaccgcc ccacgcagag caggaaccga tagcgataat
     9181 cgctgctgcg ccttctgccg ctttgcggat gtgatccaca atcggctcac cagcaaccat
     9241 gcaataaata ccgttatctt ttaatgggat ggaaccatcc acaaccaaca catactgccc
     9301 tttgtacttc tcaagtgcgt tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga
     9361 aagcacttcg tgatactcca gagagatagt ctccagcacg aggttttcta ccgttggatg
     9421 cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac
     9481 tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa
     9541 ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg
     9601 agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga agacctggca
     9661 tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt
     9721 attatggtta tcgatatcat gatactgcgg cggggcgcta aaacgaaggg aagaagcatt
     9781 agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg
     9841 gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc
     9901 tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga
     9961 tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg tttataatcg
    10021 gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt
    10081 aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatatc gccatatata
    10141 gcgatatttt aggcaaagaa cgacttttgc gcacgctatt ttttcatgct tgtttaagac
    10201 gtaatccagt aagagataaa cctatcagga gagatattta tcgaaccatt tcagtgtcct
    10261 ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc
    10321 gtgattaacc cccggataga tatacgcctc ataaacctta ttattggctt tcaacgccgc
    10381 ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa
    10441 aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcaccgc
    10501 gcaggccagt tccggatacg cgacagccgc cgcgttcgat acgccaccgc catagcaaaa
    10561 tccggtaata cccactttgc ctgccgcttg tggatagcgt tgcataaact caattgcggc
    10621 aaagaaatca ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc
    10681 atcatttccc ggataacctc caacggaact taagccgtca ggtgccaggg cgatataccc
    10741 cgccttcgcc actcgccgtg ccacatcttc gatatacgga ttcagtccac gattctcatg
    10801 caccaccacc acggctggcg ttttgccgct catttttgcg ggtttcacca gataaccccg
    10861 tacctcgccg tgaccatttg gcgaaggata catgatgtac tcagcaacaa tctccgggtc
    10921 ggtaaactct acctgagtcg ccagcgcata atttggcttg agcaaatcaa acaacgctaa
    10981 tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg aggaactcac gtttcgaaat
    11041 tttcccgtga gcgtaatagt cgtagtaatc caacaactct tgtgggaaat ctttggcggt
    11101 taaacgcggc atcgttgcac tcctcagttg gtgttttttt aagcaaagca taagcacgtg
    11161 tttttgccca gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg
    11221 ctaaactaga aacattgttt cgaaattgaa cggtggaaag gagaggtcat ggtctggtta
    11281 gcgaatcccg aacgttacgg gcagatgcaa taccgctatt gcggaaaaag cggtttacgc
    11341 ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca
    11401 cagcgtgcga tcctgcgtaa agcgtttgat ttgggcatta cgcactttga tttagccaac
    11461 aattacgggc cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgggaggat
    11521 tttgccgctt atcgcgatga actgattatc tctaccaagg ctggctacga tatgtggccc
    11581 ggcccttacg gctctggcgg ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg
    11641 aagcgtatgg ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg
    11701 ccgatggaag aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat
    11761 gtcgggatct cctcttactc gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag
    11821 tggaaaattc cgcttttaat tcatcaacct tcgtacaatt tactgaaccg ctgggtggat
    11881 aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg gctgtattgc ctttactcct
    11941 ctggctcagg gattgctgac cggaaaatat ctcaacggca ttccgcaaga ttcacggatg
    12001 catcgtgaag ggaataaagt tcgtggtctg acgccgaaaa tgcttaccga agccaacctc
    12061 aacagcctgc gcttattgaa tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg
    12121 gcgttaagct ggttgctgaa agatgatcgc gtgacgtcgg tattgattgg tgccagccgc
    12181 gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag
    12241 ctggcgcaga ttgatcagca tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc
    12301 gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg
    12361 acgagtcagt cggtcaagat agcccatcac aaatgcagaa agcacaaacg tcagatggat
    12421 aatgacgtac cacatcagtt tgttatccgg gacatttttc gcatccataa agacgcgcag
    12481 taagtgaatg gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc
    12541 gtccattttc cccagccagt tcagcttctc tttgttctcg gagatatcca gctgtgagac
    12601 gaaattctca taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc
    12661 caccagcgac agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag
    12721 tacgtgaata atctcctgga agaacttcag cgccagggca actaacgcca gcgaaaggcc
    12781 aaagtacacg ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc
    12841 catacattcc tgtcgtgtag ttaaaacggg caacagtata tcgcaattga gtaaacagca
    12901 cgcaacagca aatcaacata tacgttaaac ctttgtcgca taattctgag gtttagggat
    12961 cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc ttaaaggacg ttgaacctga
    13021 agggagaaaa acgatgtctc atttaaaaga cccgaccacg cagtattaca ctggtgaata
    13081 tcccaaacag aaacaaccga cgccaggcat ccaggcgaag atgacaccgg taccggattg
    13141 cggcgagaaa acctatgttg gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg
    13201 gggcgattcc ggaataggtc gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt
    13261 ggcgatcagt tatcttcccg tggaagaaga agacgctcag gatgtgaaaa agatcattga
    13321 agaatgcgga cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aattcgcccg
    13381 ttcgctggtt cacgaagcgc acaaggcgtt aggcgggctg gatattatgg cgctggtcgc
    13441 cgggaaacag gttgccattc cggatattgc agacctcacc agcgaacagt ttcaaaagac
    13501 ctttgccatt aacgttttcg cgctgttctg gctaacccag gaagcgatcc ccctgctacc
    13561 gaaaggtgca agtatcatca ccacttcgtc aatccaggca taccagccaa gtccgcattt
    13621 actggactat gcggctacga aggcggcgat tctgaactac agccgtggcc tggcaaaaca
    13681 ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca ggccctatct ggacggcact
    13741 gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag tttggtcaga aaacgccgat
    13801 gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga
    13861 gtcgagctac gtcaccgcag aagtgcacgg cgtgtgcggc ggcgagcatt taggttaaaa
    13921 aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa ttcactctta cctgatgtgg
    13981 cgaaaacgcc ttatccggcc tacaaacgca cgaagattca acatgttgca atagattttg
    14041 taggcctgat gagcgtagcg catcaggcaa ttctacttta cttcgctttg gcggtttctt
    14101 cgccgaccag acctatcttc aggtaacccg cctgatgcag cgtatccatt accttcatca
    14161 acgtctcgta atcgacggtt ttgtccgctc ggaagaagat ggtggtgtct ttcttgcctt
    14221 cggttaacgc attcaacgcc gtaatcattg tttcatcggt gaccggatcg ttaccgataa
    14281 acatcgagtt gtctgccttc accgacagat aaaccggttt ttccgggcgc ggctgcggcg
    14341 tgctggtaga agcaggcaag ttcaccttca catctaccgt cgctaacggt gccgccacca
    14401 taaagataat cagcagaacc aacatcacgt cgataaacgg cgtcacgttg atatcatgca
    14461 tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc catcggacat tatcctgcgc
    14521 gtaatttttg tgcgacacga accggatgcg cagcggcgct ggcttccaga tccaggtcac
    14581 ggctttgcag caacaatacc tgcgctgcaa catcacccag catcgcttta aagccgccaa
    14641 tctggcgtgc aaatacgtta tagataacga ccgcaggaat cgctgccacg aggccgattg
    14701 ccgttgctaa cagagcttct gcgatacccg gcgcaacgac tgccaggtta gtggtctgcg
    14761 tttgcgcgat accaataaag ctgttcatga tgccccagac cgtaccaaac agaccaacga
    14821 acggagaaat cgcgccgatg gttgccagat agccgttacc gcggcccatt tgacgaccca
    14881 ctgcggcgac ccgacgctcc agacggaagc tggtacgttc tttaatacct tcgttatcgt
    14941 cgctgccttc tgacagttcc agctcattct gcgcttcatt gagcaaatgc aggcttaagc
    15001 ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt taaggaacgc gcttcagcca
    15061 gcagttgctg ctcgcgctta aggcgacgct tctgattgaa gaactctacg ctcttactga
    15121 agaagattgc ccaagtgact acggaggcca aaataagccc aatcatcacg cacttaacga
    15181 caatatcggc gtgctgatac ataccccaga cggaaaggtc cgtctgcatc aaattattac
    15241 ccactgtgta tctccaggac gcaagtcaca aaatctgcgc ataataatat caaaatgacg
    15301 tcgaattgat agtcgttctc attactattt gcatactgcc gtacctttgc tttcttttcc
    15361 ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt ctgctttatg
    15421 ccaattcttc aggatgcgcc cgcgaatatt catgctagtt tagacatcca gacgtataaa
    15481 aacaggaatc ccgagatggc ggacaaaaag cttgatactc aactggtgaa tgcaggacgc
    15541 agcaaaaaat acactctcgg cgcggtaaat agcgtgattc agcgcgcttc ttcgctggtc
    15601 tttgacagtg tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg cgagttgttc
    15661 tatggacggc gcggaacgtt aacccatttc tccttacaac aagcgatgtg tgaactggaa
    15721 ggtggcgcag gctgcgtgct atttccctgc ggggcggcag cggttgctaa ttccattctt
    15781 gcttttgtcg aacagggcga tcatgtgttg atgaccaaca ccgcctatga accgagtcag
    15841 gatttctgta gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt tgatccgctg
    15901 attggtgccg atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt tctggaatcg
    15961 ccaggctcca tcaccatgga agtccacgac gttccggcga ttgttgccgc cgtacgcagt
    16021 gtggtgccgg atgccatcat tatgatcgac aacacctggg cagccggtgt gctgtttaag
    16081 gcgctggatt ttggcatcga tgtttctatt caagccgcca ccaaatatct ggttgggcat
    16141 tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt gctgggagca gctacgggaa
    16201 aacgcctatc tgatgggcca gatggtcgat gccgataccg cctatataac cagccgtggc
    16261 ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa agtggctgaa
    16321 tggctggcag aacatccgca agtagcgcga gttaaccacc ctgctctgcc tggcagtaaa
    16381 ggccacgaat tctggaaacg agactttaca ggcagcagcg ggctattttc ctttgtgctt
    16441 aagaaaaaac tcagtaatga agagctggcg aactatctgg ataacttcag tttattcagc
    16501 atggcctact cgtggggcgg gtatgaatcg ttgatcctgg cgaatcaacc agaacatatc
    16561 gccgccattc gcccacaagg cgagatcgat tttagcggga ccttgattcg cctgcatatt
    16621 ggtctggaag atgtcgacga tctgattgcc gatctggacg ccggttttgc gcgaattgta
    16681 taacattgcc acttttggac aattttgcag acatttaatt gtgaaaagtc ttaaattgtt
    16741 gcgtccggga tcaaggcgtc ccggacgaat caggagtaca ataggcagat aaaggcttaa
    16801 acgctgttcc acaggaaagt ccatggctgt tattcaagat atcatcgctg cgctctggca
    16861 acacgacttt gccgcgctgg cggatcctca tattgttagc gttgtttact ttgtcatgtt
    16921 tgccacgctg tttttagaaa acggcctgct gcccgcctca tttttgccag gcgacagctt
    16981 gttgatactg gcaggcgcat tgattgccca aggggttatg gattttctgc ctacgattgc
    17041 gattctgacc gccgcagcaa gtctgggctg ctggctaagt tatattcagg ggcgctggtt
    17101 agggaatacc aaaacggtga aaggctggct ggcacagctt cctgctaaat atcaccagcg
    17161 cgccacctgc atgtttgacc gccacggtct gctggcgctg ctggctggac gttttcttgc
    17221 atttgtccgt acgctgctgc caaccatggc gggaatttcc ggtctgccaa accgccgctt
    17281 ccagtttttc aactggttaa gtggattgct gtgggtcagc gtggtaacca gttttggcta
    17341 tgccttaagt atgattccgt tcgttaaacg ccatgaagat caggtaatga cgttcctgat
    17401 gatcctgcca attgccttgt taaccgctgg cttgttaggc acgctgtttg tagtgattaa
    17461 aaaaaaatac tgtaacgcct gacgattttc cccgttcccg gctgctgtac cgggaacgta
    17521 tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc acaccgaagt
    17581 aacgtttaaa ctcacggcta aattgcaatg cgctttcata gtcgacgcgc atcgctgctg
    17641 cgctggcctt catgccgtca tggatgatca tcatccgcgc cttatgcaga cggtaattct
    17701 tcaaatactg caacggcgag gtgctggtga cagacttaaa attatgatgg aacgccgata
    17761 cgctcatgtt ggcttctgcc gccagttgct cgacgctcag gttttcggtg tatttattct
    17821 caatccgttt cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg accagcgcca
    17881 gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg atgatctgtt
    17941 tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc tccgccgcgc
    18001 ataaaatctc ttctgataac gtggcggagt taatcccgct ggctgccatc gacggctgga
    18061 aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa ctgcaaaata tcgacattga
    18121 gacgcaatcc tgccagcggc acctctgacg tggcataggt ttcgcactca aacggcaacg
    18181 gcaccgtcag cagcaggtat tcattggcat cataacgaaa cacgcgttca ttgatataac
    18241 cgattttatg cccggaaaag agaattatga tgccaggctc gtacatcacc ggtgtacgtg
    18301 cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa cagttctgac aaactatttt
    18361 ctttattttt cagtttatta actttatccg ccagcaagcg gcaaatctct tcacgtttca
    18421 tatcgcgtaa tttcttagga ataatgcggc aatttgattg tgcgcaattt tgtagcattt
    18481 ctccagcact ctggagaaat aggcaagaca ttggcagaaa tgagcattga gagccagggc
    18541 gctggcgatc acaatgaaaa acatcaggca gatcgttctc tgccctcata ttggcccagc
    18601 aaagggagca agtaatgaac aactttaatc tgcacacccc aacccgcatt ctgtttggta
    18661 aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta ttgattacct
    18721 acggcggcgg cagcgtgaaa aaaatcggcg ttctcgatca agttctggat gccctgaaag
    18781 gcatggacgt gctggaattt ggcggtattg agccaaaccc ggcttatgaa acgctgatga
    18841 acgccgtgaa actggttcgc gaacagaaag tgactttcct gctggcggtt ggcggcggtt
    18901 ctgtactgga cggcaccaaa tttatcgccg cagcggctaa ctatccggaa aatatcgatc
    18961 cgtggcacat tctgcaaacg ggcggtaaag agattaaaag cgccatcccg atgggctgtg
    19021 tgctgacgct gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc tcccgtaaaa
    19081 ccacaggcga caagcaggcg ttccattctg cccatgttca gccggtattt gccgtgctcg
    19141 atccggttta tacctacacc ctgccgccgc gtcaggtggc taacggcgta gtggacgcct
    19201 ttgtacacac cgtggaacag tatgttacca aaccggttga tgccaaaatt caggaccgtt
    19261 tcgcagaagg cattttgctg acgctaatcg aagatggtcc gaaagccctg aaagagccag
    19321 aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg aacggtttga
    19381 ttggcgctgg cgtaccgcag gactgggcaa cgcatatgct gggccacgaa ctgactgcga
    19441 tgcacggtct ggatcacgcg caaacactgg ctatcgtcct gcctgcactg tggaatgaaa
    19501 aacgcgagac caagcgcgct aagctgctgc aatatgctga acgcgtctgg aacatcactg
    19561 aaggttccga cgatgagcgt attgacgccg cgattgccgc aacccgcaat ttctttgagc
    19621 aattaggcgt gccgacccac ctctccgact acggtctgga cggcagctcc atcccggctt
    19681 tgctgaaaaa actggaagag cacggcatga cccaactggg cgaaaatcat gacattacgt
    19741 tggatgtcag ccgccgtata tacgaagccg cccgctaagc tttttacgcc tcaaactttc
    19801 gttttcgggc atttcgtcca gacttaagtt cacaacacct caccggagcc tgctccggtg
    19861 agttcatata aaggaggaac gtatggctaa tccaaccgtt attaagctac aggatggcaa
    19921 tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt aatgaggaag taatcaccgc
    19981 cattcaaaaa gcgttagaag tgggttatcg ctcgattgat accgccgcgg cctacaagaa
    20041 cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag aactgttcat
    20101 caccactaag ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc tcgacagcct
    20161 gaaaaaactc cagcttgatt atatcgacct ctacttaatg cactggcccg ttcccgctat
    20221 cgaccattat gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg gattaatcaa
    20281 aagcatcggc gtgtgcaact tccagatcca tcacctgcaa cgcctgattg atgaaactgg
    20341 cgtgacgcct gtgataaacc agatcgaact tcatccgctg atgcaacaac gccagctaca
    20401 cgcctggaac gcgacacaca aaatccagac cgaatcctgg agcccattag cgcaaggagg
    20461 gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca gataaatacg gcaaagcccc
    20521 ggcgcagatt gttatccgct ggcatctgga tagcggcctg gtggtgatcc cgaaatcggt
    20581 cacaccttca cgtattgccg aaaactttga tgtctgggat ttccgtctcg acaaagacga
    20641 actcggcgaa attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc ctgaccagtt
    20701 cggcggctaa catgcaaatt ctcccggtga cggtaatgtt ccgctaccgg acttttcaga
    20761 aatcatttat tcccctcgcg tcccgcccgt tgttactctt ccttgttcag gaatgccaaa
    20821 tataaggaca tcatcatgc
//
LOCUS       DAZPBM010000065        20818 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPBM010000065 DAZPBM010000000
VERSION     DAZPBM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.065"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHUZQ_002172"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHUZQ_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1025869.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="ACHUZQ_002173"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="ACHUZQ_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHUZQ_002174"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHUZQ_002174"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR1025870.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHUZQ_002175"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHUZQ_002175"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1025871.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHUZQ_002176"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHUZQ_002176"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1025872.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHUZQ_002177"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHUZQ_002177"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1025873.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHUZQ_002178"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHUZQ_002178"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1025874.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="ACHUZQ_002179"
     CDS             8330..10048
                     /locus_tag="ACHUZQ_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1025875.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHUZQ_002180"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHUZQ_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1025876.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHUZQ_002181"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHUZQ_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1025877.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="ACHUZQ_002182"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="ACHUZQ_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1025878.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHUZQ_002183"
     CDS             complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHUZQ_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1025879.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHUZQ_002184"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHUZQ_002184"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR1025880.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHUZQ_002185"
                     /pseudo
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHUZQ_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHUZQ_002186"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHUZQ_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1025881.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHUZQ_002187"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHUZQ_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1025882.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="ACHUZQ_002188"
     CDS             complement(20443..20718)
                     /locus_tag="ACHUZQ_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR1025883.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4689 a         5516 c         5698 g         4915 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggtcagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaactg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggt ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctacgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagctt
//
LOCUS       DAZPBM010000066        20354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPBM010000066 DAZPBM010000000
VERSION     DAZPBM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.066"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            287..787
                     /gene="tssB"
                     /locus_tag="ACHUZQ_002189"
     CDS             287..787
                     /gene="tssB"
                     /locus_tag="ACHUZQ_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR1025884.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            822..1046
                     /locus_tag="ACHUZQ_002190"
     CDS             822..1046
                     /locus_tag="ACHUZQ_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025885.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1097..2572
                     /gene="tssC"
                     /locus_tag="ACHUZQ_002191"
                     /pseudo
     CDS             1097..2572
                     /gene="tssC"
                     /locus_tag="ACHUZQ_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2579..2992
                     /gene="tssE"
                     /locus_tag="ACHUZQ_002192"
     CDS             2579..2992
                     /gene="tssE"
                     /locus_tag="ACHUZQ_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1025886.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2996..4846
                     /gene="tssF"
                     /locus_tag="ACHUZQ_002193"
     CDS             2996..4846
                     /gene="tssF"
                     /locus_tag="ACHUZQ_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1025887.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4810..5892
                     /gene="tssG"
                     /locus_tag="ACHUZQ_002194"
     CDS             4810..5892
                     /gene="tssG"
                     /locus_tag="ACHUZQ_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR1025888.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5917..7197
                     /gene="tagH"
                     /locus_tag="ACHUZQ_002195"
     CDS             5917..7197
                     /gene="tagH"
                     /locus_tag="ACHUZQ_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR1025889.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7194..7718
                     /gene="tssJ"
                     /locus_tag="ACHUZQ_002196"
     CDS             7194..7718
                     /gene="tssJ"
                     /locus_tag="ACHUZQ_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR1025890.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7721..9052
                     /gene="tssK"
                     /locus_tag="ACHUZQ_002197"
     CDS             7721..9052
                     /gene="tssK"
                     /locus_tag="ACHUZQ_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR1025891.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9057..9818
                     /gene="icmH"
                     /locus_tag="ACHUZQ_002198"
     CDS             9057..9818
                     /gene="icmH"
                     /locus_tag="ACHUZQ_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR1025892.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9827..12610
                     /gene="tssH"
                     /locus_tag="ACHUZQ_002199"
                     /gene_synonym="clpV"
     CDS             9827..12610
                     /gene="tssH"
                     /locus_tag="ACHUZQ_002199"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR1025893.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAEPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            12607..13350
                     /gene="vasI"
                     /locus_tag="ACHUZQ_002200"
     CDS             12607..13350
                     /gene="vasI"
                     /locus_tag="ACHUZQ_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR1025894.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13355..14767
                     /gene="tssA"
                     /locus_tag="ACHUZQ_002201"
     CDS             13355..14767
                     /gene="tssA"
                     /locus_tag="ACHUZQ_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR1025895.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14876..18310
                     /gene="tssM"
                     /locus_tag="ACHUZQ_002202"
                     /pseudo
     CDS             14876..18310
                     /gene="tssM"
                     /locus_tag="ACHUZQ_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18321..19770
                     /locus_tag="ACHUZQ_002203"
                     /pseudo
     CDS             18321..19770
                     /locus_tag="ACHUZQ_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19697..20174
                     /locus_tag="ACHUZQ_002204"
                     /pseudo
     CDS             19697..20174
                     /locus_tag="ACHUZQ_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
BASE COUNT         4818 a         5563 c         5612 g         4361 t
ORIGIN      
        1 tatatacttc aataagttac acaaaaactc tatatttaaa ttattgccat aatacacctg
       61 acgcgaattg cacaggttca cacaatctat ttaacgcttg cagaataccc ctctttacga
      121 tcgataaaaa gtccttttct tatttatttt gaagcgtaaa atgcggggtg atgaaggagt
      181 ggtgatttta ttttcaccgc cattagggcg gcattgccga tatttttttg cggtacaaat
      241 atgccgtgtt catggcctgt acgagataac atgacggatc acttttatga gcaaaaaatt
      301 tgaaggcagc gtcgcgcccc gcgagcgtat taatatatcg tatgtcccaa aaacagatgg
      361 ccagacggct gaagtggaac ttccgcttaa catgctcgtt gtcggcgata ccggaaatac
      421 gcaggaaaca tccccactgg atgaacgtca ggcagtttcc gttaacaagc acaatttcgg
      481 tgcagtgatg gctgaagcag ccattggcct caattttact gtccccgcca cgctgaaggg
      541 aagcacaaca gacgatgaac tgaatgtggc gctgaatatc aaatcactgg atgatttttc
      601 accagatagc gtggcccggc aggttccgga agtgaataaa ttgctggaac tgcgtgaagc
      661 actgactgca ctgaaggggc cgatgggtaa ccttccggca ttccgtaccc agctccaggc
      721 attgctggaa aatgaagagt cccgcgagca gttgctcaaa gaaatcggtc tggtgagcaa
      781 taaataacca ccactatttc attaaacagg gttatccagg catgacggca cacaaaaata
      841 tctccgggac ataccacctc tctgttgcag acattctgca ggtggtttac caggtatgtt
      901 tcagcccgtc cgttgaaatc aaccaggatg gtgtggctgc gctgattaca acactggatc
      961 gccgtatcag tgatctattg gatgagatta tccatttttg tgagtttcag cagagcgcgt
     1021 cgcactggca gcgcgtactt cactgatggc gaaaagccgg tttcagaaac acagctaaaa
     1081 cagaaggatt ttcagtatgt ctttacagga agaggaactc gtttccagtc atgccgggca
     1141 gcctgagcag gcatcgtcgc tgcttgatca aatcatggca cagacccgta ttcagcccgg
     1201 ttcggaaggt tatgacgttg ccctccaggg tgtgacagcc tttattgcca gcatcctgca
     1261 atccactgca tctgccgaac cggtcaataa actggccgtg gacagcatga ttgcagatat
     1321 tgatgaacgc atcagccgcc agatggatgt catcatccat gcgcctgcct ttcagcaggt
     1381 cgagtcgttc tggcgctcac tgaaaaccat ggtggatcgc gttgatttcc gtgaaaacat
     1441 caaggtcaac gtgcttcatg tcaccaagca ggaacttctg gaagactttg agtttgcgcc
     1501 agaaattatt cagtccggat tttataagca cgtttattcg tccggtttcg gacagtttgg
     1561 cggtaaaccc atcgctgccg tgctgggcgc ttatgagttc aaaaataccg cgcctgacat
     1621 gaaactgctg caatacgtca gtgccgtggg cgcaatggcg cacgcaccgt tcctgtcctc
     1681 cgtttccccg gagtttatgg ggctgaactc gtggaccgaa ctgcctaata tcaaagatct
     1741 ttatgccatc tttgaaggtc cggcctacac caaatggcgc gccctgcgtg actcggaaga
     1801 ttcgcgctat ttagggctta cggcaccccg cttcctgctt cgccagccct attcaccaac
     1861 agataaccct gttaagaact ttaactatta cgaagatgtc agccagaacc acgaagatta
     1921 tctgtggggt aatacggcct ggatgctggc atgcaatatc gcagacagtt ttgccaaata
     1981 ccgctgatgt ccaaacatta tcggtccgca aagcggcggc gcagtgaaag atctgccgat
     2041 gcatctgttc gaaacgatgg ggcagattca ggccaaaatc ccaaccgagg tgctggtcac
     2101 cgatcgccgc gaatttgaac tggctgaaga gggttttatc acccttacca tgcgtaaaga
     2161 ctctgataat gcagcctttt tctctgcaaa ctcggtacaa aaaccgaagc actttccggg
     2221 aaaagatgca gaaaccaact ataagctggg tacgcagctt ccttatctct tcatcatcaa
     2281 tcggttagcg cactacatca aggtgttgca gcgtgaacaa ctggggtcat ggaaagaaag
     2341 gagcgactta gaaagagagc ttaatacctg gatccgtcag tatgttgccg accaggaaaa
     2401 cccgcctgca gacgtgcgca gccgcaaacc tctgcgcgct gccaaagttg aggttatgga
     2461 tgtagaaggc gaacccggct ggtaccaggt tgcgctaagc gtgagacctc attttaaatt
     2521 tatgggggca aattttgagc tttccctggt tggccggtta gacagggagt aacacaccat
     2581 gataccggat cgggaaagaa ccacaggttc tctttttgag cgtatggaag cctcttcggc
     2641 cagaaaccgg cagggaggca gtattcattc gctcaggcaa tccattcgcc agaacctacg
     2701 aaacattctt aatacccggt ccggtagttg ccggggtgcg cctgagctgg gcattgatga
     2761 accggaaggg gctgaaaact tccgcgaatc catgagtcgg gccatcgagc aatgtataga
     2821 acgctatgaa ccccggatct cacacgctga agtgcaggca gtggtttcat ccgcctccag
     2881 cccgctggat atgacgtttc atattacagc ctgggtgaca tttaacgaaa cgcatgaggt
     2941 gcttgaattt gatatggcac cgaatggcag ccagcattac cgggtggact gacaaatgga
     3001 atttgaagaa cgctatttcc gggaagaact cgattacctg cgccagctta gcaagctgct
     3061 ggcaacggaa aaaccccatc tggcccgctt cctggccgaa aaagatgcag atccggatat
     3121 tgaacgcctg ctggaagggg tggcttttct taccggcaat ctccgccaga aaattgagga
     3181 tgaattccca gaactgacgc acgggcttat taagatgcta tggcctaatt acctgcgtcc
     3241 ggttccggca atgaccctta ttgaatatac gccggatatg gataagtctt ctgtaccggt
     3301 gttaatcccc cgtaatgagc agtttacaac caacgccggg gaaatcagag ttgatgaagt
     3361 gctgccctct gatgctaaaa aggaggagcc gcctccctgt accttcaccc tctgccggga
     3421 tatctggctg ctgcccgttc gcctggagca gattgaaaac cgcagtacga cccgtaatgg
     3481 tgttatcaac atcacctttt cggtcgcacc gggaacggac ttccgcacgc tggatctgaa
     3541 caaacttcgc ttctggctcg gcaatgacga caactatacc cgtgaccagc tttatttatg
     3601 gttctgcgaa tacttgcagg gtgccgacct gactgtgggt gaacagcata ttcgcctgcc
     3661 tgagtttatg ctaaaagctg tcggttttga gccgcaggat gccatgctgc cctggccgaa
     3721 aaacgtccac agcggctacc ggatccttca ggagtatttc tgttaccccg atgcgtttct
     3781 cttttttgat ctttgtggtt gtccggcttt gcctgacgga ttgcaggcgg aattctttac
     3841 cctgcaactg cgtttttcgc gccctttgcc cgtggacatc cggctgcgcc gcgattccct
     3901 gcgcctgtat tgcgcacctg ccattaattt atttatccac catgcagaag ccatcacgct
     3961 ggacaaccgg cgggcagact atccgctggt tcccagccgc cattacccac aacattacga
     4021 tgtattttcc gttaacagtg tggtgagcca ggtccaggat atgttcagga aaaaagatct
     4081 ggggcgtcct gtttcgacgc aggccgcgcg ccagtggcca gcctttgaaa gtttcagcca
     4141 tcagatagaa tacagccgga agcgggaagt ggtgtactgg catcaccgga ccaaaacatc
     4201 cctgttccat cgcggctttg atcataccct tgcctttata catgctgatg gcagttatcc
     4261 gtcagacgaa tctctgctca gtaatgaagt ggtttcggta tcgctgacct gtaccaaccg
     4321 tgagcttccg tcacaaattc gttccggcga tatcaccggc acaaccggaa aaaatgcggc
     4381 tgttgcttca tttcgcaaca ttacccgccc gacgcaacca ctctggccgg tcattgatgg
     4441 cagcctgcac tggcccctac tctccgccat gaacctgaat tatctgtcat tactggatac
     4501 ggacgcgctg aagcaggtca tcgccaactt tgatcgccac gcaatccatc atccgcagac
     4561 ggcacggctg tcacaacaaa agctggatgc cattgagcgt ctggagaccc gccccgttga
     4621 tcgcctgttt acgggtattc ccgtccgggg gctggcttcc acgctgtatc tgcacccgga
     4681 gccgtttgtc tgtgaagggg aaatgtatct gctcggtacg gtgctttcgc attttctgtc
     4741 gctgtacgcc agcgttaact cattccacat gctgaccgtt gtgaacacag aaagccagga
     4801 gacatggaaa tggacggaaa gaatcgggca gcatcctctt atctgagtcc gggaaatccc
     4861 ccggcagata aagagcagaa tgatccgctg gcacaggttt tccataatgc ctgctcttac
     4921 aatttttttg cgatggcgga gctgttgcac cgcctggcaa agggtgaaaa ggggacgcca
     4981 gaattatccc tgcgcgacga tccggcacag gaaaccctgc gtttcagcgc cgatgccagc
     5041 cttgccttcc cgtgcagtga tatcagtgcg ctgaaacggg atacgtcagg ggcattcagg
     5101 atgaccacca cctttatggg gttacagggg agccagtcgc ctctccccgg ttattacctc
     5161 gctcacctgg cctggaaagc ggtacatgaa caaagtccgg taggtgattt tctggatatg
     5221 ttcagccacc gcctgaccca gtttgtctgg catatctggc gtaagtaccg ctatcacatc
     5281 agttttcgta acggcggtgt ggatgcgttc tcgcagcgca tgtactccct tgtcgggctg
     5341 ggccaccgcc agcttcgcga taagctggcg attaatcaca gcaaaatgct ggcgtattcc
     5401 gggatcctgg caaatccggg acgatccccg gaaattatct gtggcctggt gtcccactgc
     5461 tttgatttgt cagaagtgac actgcaaaac tggcaacgtc gcaaggtgga tattgaaccg
     5521 gatcagcaaa atagtctggg gagctattct cttaaaaatg gcgaaaaact ggcgggaaga
     5581 tcggtactgg gcaactttgt tctgggtaca cgagtgcccg atctcagcgg aaaattccag
     5641 ctcagtatta ccagcctgac ccgaaaacag ttcctctctt ttctgccgtc cggcgaaaac
     5701 ttcctgccac tgacgatgtt cgtgtccttc atcctgcgcg atcagcttgc ctgggactta
     5761 catctggggc tggcgccgga gcaggtgggc gcaatgcgcc tgggcgataa caaaagtgcg
     5821 ctgctgggct ggaccagctt cctcggcaca ccggaagaac gaccctcagt caccatcagg
     5881 gtacggtcgt aaattatgat aatcacggag cagaatatgc cggaagagaa actacagacg
     5941 ctttcattgc aggtcattaa cggcagtgag ctggaaagcg ggcgggcggc gcgctgtctg
     6001 ttcacacagc agggaaatgt cggccatggc cccgaatgcc actggtcggt acaggatcgt
     6061 cagcagagca ttccggccca ggcttttacc gttatcctgc acgatggcac attttgtctg
     6121 cgcccgcaga cggcacaact gtggctgaat caggcaaaag tcacagcaac atcagaccta
     6181 atacagttgc gccagggcga tgagatccag atcggacggc tgatggtgag ggttcatctg
     6241 aaccggggag atattcccca ttacgatgag gaaatggcca ctcccgaaac catcgttacc
     6301 aatcgcgata tgctcacgga taccctgcta tcaacggagg gtgcgccaca ctatccggga
     6361 atgactcacc ggcaccagct tgcagacacc gtggtaaacg gtttttctgc cgatccactc
     6421 caggctcttc agtccgaaag cctgattacc acgggcgatc cgctttcagg cattgcggct
     6481 gtccggccat cggcaccgct gtccgatccg gcaagtaatg gggggatcaa tactccgttt
     6541 atggatctgc cgcccattta tgccagcccc ggcgatcgta atgacgacgt ctctgcggca
     6601 gaaatggcgc aacgccacgt tgcggtcacc cccttactgc gcggtcttgg cggctcgctt
     6661 accatgagca attccgacga tgcggatgat tttctggagg aggccggacg aacgttacag
     6721 gccgcaataa aaggtctgct cgatttgcag cagcagcgta acagcctctc agacaaacat
     6781 ttgcgcccgc tggaagataa cccgctgcgc ctgaacatgg attacgccac cgcgctcgac
     6841 gtaatgtttg ccgaaggtaa aagcccggta catctggcgg ctcccgccgc cgtcagtgaa
     6901 agcctgcgca atgtccgcca ccacgaagaa gctaaccggg cagcgattgt ggagtcgctt
     6961 cgtgtcctgc tggatgcttt ctcaccacaa aatctgctgc gccgctttgt gcagtaccgc
     7021 cgcagccatg aactgcgcca gccgctggat gatgccggag catggcaaat gtacagccat
     7081 tattacgaag aactggcctc cgatcgccag caggggtttg agatgctgtt taacgaggtc
     7141 tacgcccagg tctatgaccg ggtgcttcgt gaaaaacagc gggagccgga agcatgaaca
     7201 ggaaggcgtt tctggcctgc gtgctgatgt gcgtcatatt aaccggctgc gaaacggcga
     7261 aaaaaatcag tgaggtgatc aaaaacccgg atatccaggt cgggagcctg aaggagcaac
     7321 cttcggaaat aaccgtcacc ctgctgaccg aaccggacac caataccaat gcggaaggtg
     7381 aatcagccgc cgtggatgtg caactggtgt acctgactga cgactcaaaa ttgctggcag
     7441 ccgactatga ccagatagcc agcacgccgc taccggatgt gctgggtaaa aactatatcg
     7501 accaccagga tttcaacctg ttgccggaca ccatcaaaac cctgccgccg gtaaagctgg
     7561 atgagaaaac gcagtttatt ggtgtcgtgg cctatttctc cgatgaccag gccactgaat
     7621 ggaaacaaat cgagacggtg gaaggaatcg gccaccatta ccgcctgctg gtgcatgtac
     7681 gtcaaagcag catcgaaatg aaaaaagagg atgaataatt atggcaacca cccgcaacaa
     7741 ggtgatgtgg caggaaggga tgctgatgcg tccacaccat ttccagcagc agcagcgtta
     7801 caacgactac ctggataacc agcgtttccg ggccatgaat gatttatcct ggggatttac
     7861 cgaactcacc ctcaacaatg aactgctggc gcagggtaag atcatgattg acagcgcgtc
     7921 aggcacactg cccgacggca ccgtcttttc tatccccgac caggacgcac tgcccgatcc
     7981 gctgcacccg caaaattttc cggacgaaag aagccgcaat atctacctcg ctctgccggt
     8041 cgccagtgat gtgagaaatg aaatcagcga cggacggcga atcgggcgtt accggctgaa
     8101 ttatgccgat gtccgggatt tgcattcaga agaaggtgac gcgcgaacat tgacgctggg
     8161 tcaactgacg ccgcgcatta tgagcggtgc agaagatatg agcgcctata ttacgctccc
     8221 gctttgccgt atcagcgatc gccatgccga tggctccctg acgttggatg acgattttat
     8281 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     8341 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     8401 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     8461 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     8521 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
     8581 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
     8641 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
     8701 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
     8761 cgtggcggca aatatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
     8821 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atcccctgta ttccattgcg
     8881 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
     8941 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
     9001 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
     9061 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
     9121 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
     9181 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
     9241 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
     9301 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
     9361 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
     9421 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
     9481 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
     9541 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
     9601 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
     9661 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
     9721 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
     9781 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
     9841 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
     9901 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
     9961 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    10021 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    10081 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctggctgc tggcgtcggc
    10141 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    10201 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    10261 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    10321 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    10381 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    10441 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg
    10501 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    10561 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    10621 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    10681 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    10741 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    10801 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    10861 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    10921 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    10981 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    11041 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    11101 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    11161 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    11221 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    11281 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    11341 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    11401 agagtcagag tcagagtcag agtcagagca ggataatacc ggtgccgaac cggctgatga
    11461 ggccgacaga gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    11521 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    11581 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    11641 gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa
    11701 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    11761 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa
    11821 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    11881 catcaatatg tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc
    11941 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    12001 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    12061 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    12121 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    12181 cccggaaacc atgcaggaag tactgtatcc ggtgctggcc gacttcttca aacctgccct
    12241 gctggcgcgt atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat
    12301 tatcgccgga aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgtagaagt
    12361 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    12421 cgcgaggatg ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    12481 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    12541 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    12601 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    12661 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    12721 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    12781 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg
    12841 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc
    12901 cgcgccacag agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag
    12961 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca
    13021 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    13081 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc
    13141 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg
    13201 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac
    13261 aatgccgccg gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg
    13321 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    13381 tgtctggata tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa
    13441 agatgacaac ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc
    13501 ccacagtcaa ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga
    13561 gagtaaggat tttcgtctgc tggcgcatct gctaagaacc ctgcaacacg ccggtgatcc
    13621 tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc
    13681 gcagaatatg gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg
    13741 tattgaagtt ttttcacaga acgccgccac agcgcagcgc gatgcgctgt cagggcaact
    13801 ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga
    13861 tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac
    13921 tccggcggcc tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca
    13981 acccagtgct ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga
    14041 cacgctgtta aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta
    14101 ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga
    14161 tggccgtact ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct
    14221 cggtagtgcc gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta
    14281 ctggctggat ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt
    14341 ggcagacgcc atccgcgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    14401 gctgctgttt aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc
    14461 ttcgcccgac gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag
    14521 agcctgtttt gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    14581 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc
    14641 ggggctggta cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat
    14701 gatggtggca gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga
    14761 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    14821 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct
    14881 gatactggcg tggatttttc tgctggtgtg gatctggtgg aaaggcccga tgtggacgct
    14941 gtatgaagaa cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg
    15001 gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa
    15061 aatgcaaaag caacagcgcg aggaagccgt tgatccgctc agcgtggaac tgaacgccca
    15121 gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt
    15181 cctgtggcag ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact
    15241 gctgcgcaag gggtttccgt ccgacattat ttatgccccg gagggcgcac gcggcgcaga
    15301 acaacgcctg tacctcacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg
    15361 cacactctgc gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacatgccct
    15421 cggctggctg aaagaaaagc gcgcgcgcca gccgctgaac gggattattc tgacactcga
    15481 tttacccgat ctgctgaccg cagacaaacg ccgccgcgag catctgttac agacgctgcg
    15541 cagccgctta caggatatac gccagcatct tcactgccag ttaccggttt acgtggtact
    15601 gacccggctt gatttattac agggttttgc cgccctgttc cagtcactca acagacagga
    15661 tcgcgatgcg attctgggcg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg
    15721 aacagagttg aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga
    15781 tctgatggtc gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca
    15841 gggaagccgt gaaccgctgg tgtcactgct tgagggtctg cttgatggcg aaaatatgaa
    15901 cgtgatgctg cgtggtgtct atctcacctc ttcgcttcag cgtggacaga tggatgatat
    15961 attcacccag tctgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc
    16021 cctggtggac accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga
    16081 gcctaatctg gcaacagaga gccgcgcctg gctgatacgt tcccgtcgcc gcctgacggt
    16141 tttctctgcc acaggcggcg tggcagcact gctgctcatc accggctggc atcactatta
    16201 caacggtaac tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt
    16261 gccgcctccg cagggggaag atgactttgg caacctgcaa ctgccgctcc tgaacccggt
    16321 acgcgatgcc acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg
    16381 actgtaccag ggacgacgta tcgggcctta tgtggaacag acctatctgc aactgctgga
    16441 gcaacgttac ctgccctcgc tgtttaacgg gctggtcaaa gcgatgaacg ccgcgccgcc
    16501 ggagagtgaa gaaaaactcg cggtgctgcg cgtgatgcga atgctggagg acaaaagcgg
    16561 acgtaacaat caggtggtga agcagtatat ggcaaaacgc tggagcgaaa agttccacgg
    16621 tcagcgcgat atccaggcac aactgatgtc ccatcttgac tacgcgctgg ctcatactga
    16681 ctggcacgca gagcgtcagg cgggcgacgg tgacgccatc agccgctgga cgccatatga
    16741 caagcccgtg gtatcagcac agaaagaact gagcaaactg cctgtctacc agcgggttta
    16801 ccagagcctg aaaacgcggg cgctgggcgt tcttcctgcc gacctcaatc tgcgtgacca
    16861 ggtagggcca acctttgacc aggtgtttac atctgccgat gacaacaaac tggttgttcc
    16921 acagtttatc acccgctacg gcctgcaaag ctattttgta aaacagcgcg atgaactggt
    16981 tgaactgaca gcgatggatt cctgggtact taacctcacc cgcaatgtga aatacagtga
    17041 cgccgaccgt gcggaaatcc agcaccagtt gaccgagcag tatatcagcg actacaccgc
    17101 cacctggcgg gctgggatgg acaatctgaa tatccgcaat tttgagtcca tcggacaact
    17161 gaccggggcg ctggagcagg ttatcagcgg cgaccagcct ttgcagcggg cgctgaccgt
    17221 gctgcgtgac agcacacagc caggcgtctt ttctgaaaaa ctctctgcca aagaacggga
    17281 ggaagccctg gcagagccgg attaccagtt actcacccgc ctcgggcatg aattcgcccc
    17341 ggaaaacagt accctggcag tacagaaaga caaagaaagc acgatgcagg ccgtgtatca
    17401 gcaactcacc gagttgcacc gctacctgct ggcaatccag aacgcgcctg taccagggaa
    17461 atcggcgctg aaagccgtgc agttacggct tgatcagaac agcagcgatc cgatattcgc
    17521 cacccgccag atggcaaaaa cgctgcctgc tccgctcaac cgctgggttg gcagactgac
    17581 tgactaggcc tggcatgtgg tgatggtgga ggctgttcat tatatggaag tggactggcg
    17641 cgacagcgtg gtgaaaccgt ttaacgagca actggcaaat aactatccgt ttaatccgcg
    17701 ttctgcacag gatgcctcac tggatgcctt cgaacgcttc tttaaaccgg atggcatact
    17761 ggataccttc taccagcaga acctgaagct gtttatcgat aatgacctga gtctggagga
    17821 tggcgataac aacgtcatta ttcgcgaaga tattattgcg caactggaaa ctgcgcagaa
    17881 aatccgtgac atcttcttca gcaaacagaa cggtctggga acatcctttg ccgtggaaac
    17941 ggtatcgctt tcaggcaata aacgccgcag tgtactgaac cttgacagtc agttagtcga
    18001 ttacagccag ggccgtaact ataccgccca tctggtctgg cctaacaaca tgcgcgaagg
    18061 caacgaaagt aagctgacgc tcatcggcac cagcggcaac gcgccgcgca gtatcagctt
    18121 cagcgggccg tgggcgcagt tccgcctgtt ctgggccgga caactgaccg gagtacagga
    18181 tggcaacttt accgtgcgct ttagcgtgga cggtggcgcg atgacctacc gtgtgcatac
    18241 cgacacggaa gataacccgt tcagcggtgg gttgttcagc cagtttggtc tgtcagacac
    18301 actgtactga ggaggcaggg atgaatagta acgtactgac acaaactatc gttaccggca
    18361 gtgacccgcg cgggctgccg gaattcagcg ccatccgcga ggaaataaac aaagccagcc
    18421 acccgtcaca gcctgagctg aactggaaac tggtggagtc gctggcgctg gcgattttta
    18481 aagccaacgg tgtggattta cacaccgcca cctactatac gcttgcccgg acacggacac
    18541 agggactggc gggattctgc gaaggtgcgg aactgctggc ggcaatggta agccacgact
    18601 gggataagtt ctggccgcag ggcggcccgg cgcgtactga aatgctggac tggtttaact
    18661 cccgaaccgg caatattctg cgtcagcaaa tctcctttgc ggaatccgac ctaccactga
    18721 tctaccgcac agagcgggca ttgcagctta tctgcgataa gctccagcag gtggaactga
    18781 agcgcgttcc gcgcgtggag aatctgctct attttatgca gcacacgcgt aaacggcttg
    18841 aaccccagcc gaagagtaac gctgagaacg ccgcacagac cacggtcaga acgctgattt
    18901 atgccccgga aacactggca tcttccacac cagaagcggt agtgcctccc ctgcccggcc
    18961 tgcctgagat gaaagtggaa gtgcgcagtc tgacagagaa tcccccacag gccagtgtga
    19021 taaagcaagg cagtacggta agagggttta tcgcagggat cgcctgttca gtggctgtcg
    19081 cctcagcatt gtggtggtgg caggtctatc cggtgcagca gcaactgtta caggttaacg
    19141 acaccgctca gggcgcagca acggtgtgga tggcctcacc tgaactcgaa aactatgagc
    19201 gcaggctgca acaacttctt gatacctccc cggtacagcc gctggaaacc gggatgcaga
    19261 tgatgcgtgt tgccgacagt cgctggccgg aaagcctgca acagcaacag gcctcgacac
    19321 aatggaatga ggcactcaaa acccgcgcac agagtagccc gcagttgcgt ggctggttgc
    19381 agacccgcca ggacttacat gcttttgcag atctagtgat gcagcgcgag aaagagggac
    19441 taaccctttc ctatatcaaa aatgtcatct ggcaggcgga gcggggactg gggcaggaaa
    19501 cccccgttga gtctctgttg acgcagtacc atgatgcccg tgcgcagaag cagaatacag
    19561 atgcgctgga aaaacaaatt aatgagcgac tcgaaggcgt gttaagccgc tggctgctgc
    19621 tgaagaataa cgtcatgcca gaggcggcaa ccggtaccac ggccgaaaaa taattattta
    19681 attaaaggag attatcatgg caaatataag ttatttatct ttgagtggag agacacaagg
    19741 gctaatttca gcaggttgct caacgcttga ttctgtcgga aacaaagcgc agccagaaca
    19801 taaagatcat ggtttatgcg ttaatgcatt ccatctcaag aagtcaaaat gtcaatcatc
    19861 atgaattaat tatcaccaaa ccggttgata aatcctcacc attgctggca aaagcactta
    19921 gtgacaatga aaaaatggca atatgtgaat ttatactcta ccgaaccagt aaagcgggta
    19981 tttaccagcc ttattataaa ataaatctgt caaaagcccg aatatcaagt attgattttg
    20041 tcacaccgca tgctgtcctg gaaaaagaac tggaaccaca ggagagaatt gcctttattt
    20101 atgaagatat ttcctgggag catacgttag ccggtacgaa tgcaatgagt aaatggcagg
    20161 atcgtgtaca gtaacttact gtaataacat aataaattac tattgcggag agataaaatg
    20221 gctggtttaa ggaataacaa caacacacag aatgcacagt gggctgatta tgtgggtgac
    20281 attcttcggg gggctcagcc gataaaccag ctagtacctc agcaccctta tcttaacgac
    20341 gtcccactca ttga
//
LOCUS       DAZPBM010000067        20240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPBM010000067 DAZPBM010000000
VERSION     DAZPBM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.067"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..70)
                     /locus_tag="ACHUZQ_002205"
     CDS             complement(<1..70)
                     /locus_tag="ACHUZQ_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025896.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
     gene            689..1459
                     /locus_tag="ACHUZQ_002206"
     CDS             689..1459
                     /locus_tag="ACHUZQ_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1025897.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="ACHUZQ_002207"
     CDS             1450..1923
                     /locus_tag="ACHUZQ_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1025898.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHUZQ_002208"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHUZQ_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1025899.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHUZQ_002209"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHUZQ_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1025900.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHUZQ_002210"
     CDS             3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHUZQ_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1025901.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="ACHUZQ_002211"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="ACHUZQ_002211"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1025902.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHUZQ_002212"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHUZQ_002212"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR1025903.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="ACHUZQ_002213"
     CDS             7698..8612
                     /locus_tag="ACHUZQ_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1025904.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10188
                     /locus_tag="ACHUZQ_002214"
     CDS             8827..10188
                     /locus_tag="ACHUZQ_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1025905.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10237..11892)
                     /gene="tsr"
                     /locus_tag="ACHUZQ_002215"
                     /pseudo
     CDS             complement(10237..11892)
                     /gene="tsr"
                     /locus_tag="ACHUZQ_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12011..12457)
                     /gene="hpaR"
                     /locus_tag="ACHUZQ_002216"
     CDS             complement(12011..12457)
                     /gene="hpaR"
                     /locus_tag="ACHUZQ_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1025906.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12729..14018
                     /gene="hpaG"
                     /locus_tag="ACHUZQ_002217"
     CDS             12729..14018
                     /gene="hpaG"
                     /locus_tag="ACHUZQ_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1025907.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14015..15481
                     /gene="hpaE"
                     /locus_tag="ACHUZQ_002218"
     CDS             14015..15481
                     /gene="hpaE"
                     /locus_tag="ACHUZQ_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1025908.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15483..16334
                     /gene="hpaD"
                     /locus_tag="ACHUZQ_002219"
     CDS             15483..16334
                     /gene="hpaD"
                     /locus_tag="ACHUZQ_002219"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1025909.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16344..16724
                     /locus_tag="ACHUZQ_002220"
     CDS             16344..16724
                     /locus_tag="ACHUZQ_002220"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1025910.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16835..17638
                     /gene="hpaH"
                     /locus_tag="ACHUZQ_002221"
     CDS             16835..17638
                     /gene="hpaH"
                     /locus_tag="ACHUZQ_002221"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1025911.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17649..18437
                     /gene="hpaI"
                     /locus_tag="ACHUZQ_002222"
     CDS             17649..18437
                     /gene="hpaI"
                     /locus_tag="ACHUZQ_002222"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1025912.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18612..19988
                     /gene="hpaX"
                     /locus_tag="ACHUZQ_002223"
     CDS             18612..19988
                     /gene="hpaX"
                     /locus_tag="ACHUZQ_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1025913.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19998..>20240
                     /locus_tag="ACHUZQ_002224"
     CDS             19998..>20240
                     /locus_tag="ACHUZQ_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1025914.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4871 a         5432 c         5171 g         4766 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc ctgctggggc
     9961 ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt
    10021 gccagcttca tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc
    10081 cactcattcc gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg
    10141 tacatcttcc tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc
    10201 cgataacatt ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc
    10261 gctatctgcc acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga
    10321 tgtttcacgc tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc
    10381 ctgctcttcc agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg
    10441 agttacccgg tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc
    10501 atcagaagca gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat
    10561 ctccgccatt gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac
    10621 cgagtcttca atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt
    10681 acgcacttct cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc
    10741 aaccgccgcg ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat
    10801 aatatcggcg attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc
    10861 taccactttg ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg
    10921 gcgcgcattt tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt
    10981 ctcttccagc gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc
    11041 gatttcgctg gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg
    11101 catcagctct ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc
    11161 atccacctca atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag
    11221 gcgattcatt ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc
    11281 cagtacgacg atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt
    11341 atcgctgacg gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt
    11401 ctcgaaaccg tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc
    11461 acctaacagt tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat
    11521 ctctgccgct gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca
    11581 gtttttttcc gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga
    11641 accgctacca atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt
    11701 acgcgtctgc aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt
    11761 ttgtaaaaca gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga
    11821 tgtcagttgt aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat
    11881 acgttttaac atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca
    11941 aaaactttat gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga
    12001 tactaaaaag ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa
    12061 taaattcctc taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa
    12121 tctgccgata ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt
    12181 tggttagcga gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac
    12241 cgtcgcgttc catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat
    12301 acgccagatc gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact
    12361 gttgctcagt caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc
    12421 gcgcctggag caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa
    12481 aatcattaat atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt
    12541 cctgatttta tcaaaattga tgatatttta ttaacttact gatatttaat attttattat
    12601 ctttaaatgt aaatagtttg ttaattagat cacatttaca tcacttactt ttgccaaact
    12661 acgcaatcgg gaataaaagc aatcattaac atattaatga ttaagatgat cgaatccgag
    12721 gagtggtaat gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg
    12781 catggcagga agcgttccag caatccccct acaaagcccc gcctaaaact gcggtctggt
    12841 ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg
    12901 aaaaggtact gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg acgaaagtac
    12961 gtgaagaaga tgcggcagag tacatcgccg gatatgcgct ggctaacgac gtcagcctgc
    13021 cggaagagag cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca
    13081 ttggcgaaac cgtggctctc agcaatgtcg ataatctgac catctatacc gagatcaacg
    13141 ggcgtcctgc cgatcactgg aataccgccg atttacaacg taacgccgcg cagttgctga
    13201 gcgccctgag cgaatttgcc acgctgaatc caggcgacgc cattctgctc ggcacgccac
    13261 aggcgcgcgt ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa ggtttcccgc
    13321 cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac gcacaagagc ttcccaacgc
    13381 agccacaccc gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg
    13441 aactggaatt taagccaccg gaagaaccgc tggtgttcct gaaagcgcca aataccctca
    13501 ctggcgataa ccagacctcc gtgcgtccaa acaatattga atacatgcac tatgaagcgg
    13561 agctggtggt agttattggc aaacaggcgc gtaacgtcag cgaagccgat gccatggatt
    13621 atgtcgcggg ctacaccgtg tgtaacgact acgccattcg tgactatctg gaaaactact
    13681 accgccctaa cctgcgggta aaaagccgcg acggactgac gccgatgctt tcaaccatcg
    13741 tgccgaaaga ggcgatcccg gacccgcata atctgaccct tcgcaccttc gtcaacggcg
    13801 agttacgcca gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct
    13861 acttaagtga atttatgacc ctgaatccgg gcgacatgat cgccaccggc acacccaaag
    13921 gcttatctga cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc
    13981 tggtgaaccg aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc attggatcaa
    14041 cggcaaaaat gttgcaggta acgactactt ccagaccacc aatccggcaa cgggtgaagt
    14101 gctggcggat gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa
    14161 agaggcgttc ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg
    14221 tctgggcgat ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa ccgcggacac
    14281 cggcctgccg atccatcaga ccaaaaatgt gttgatccca cgcgcttccc acaactttga
    14341 atttttcgcg gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat
    14401 gctcaactac acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc cgtggaacgt
    14461 accgtttatg accgccacat ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt
    14521 actgaaaatg tcggaactct ccccgctgac cgctgacagc ctgggtgagc tggcgctgga
    14581 agccgatatt ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa ccgcagggga
    14641 tgcgctggtt cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta cggccaccgg
    14701 gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggaactgg gcggtaaatc
    14761 gccggtgctg atttttgaag atgccgatat tgaacgcgcg ctggacgccg ccctgttcac
    14821 catcttctcg atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcaaag
    14881 catctacccg gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga
    14941 tccgaacgat ccgaataccc aggttggcgc gcttattagc cagcagcact gggaaaaagt
    15001 ctccggctat atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg gcggcccgga
    15061 taaaccgtcc gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caaccgtgct
    15121 ggcagacgtt gataaccgta tgcgagtcgc ccaggaagag attttcgggc cggtcgcctg
    15181 cctgctgccg tttaaagacg aagccgaagg cttacgtctg gcaaacgacg tggagtacgg
    15241 cctcgcgtcg tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat
    15301 tgaagctggc atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc agccatttgg
    15361 cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc
    15421 ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg
    15481 atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatcactctg
    15541 aactgccagg gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat aaagagatca
    15601 gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc cactggctgg
    15661 tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg
    15721 agctgccgca ttttatccgc gatatgacct acaactacga gggcaacccg gagttggggc
    15781 agcttattgc cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aacattccca
    15841 gcctgaaact ggaatacggc acgctggtgc cgatgcgcta catgaatgaa gacaagcact
    15901 tcaaagtggt ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc
    15961 tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca
    16021 gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct
    16081 acacccgcga gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg cgcgaaggcc
    16141 agttcaaaga gttctgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca
    16201 atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg
    16261 tggagtttat caccgagcta ttcccaagct ctggcaccgg tcaggttaac gctgttttcc
    16321 cgcttcccgc gtaaggacgt tttatgccgc actttatcgt taaatgcagt gacaacatcc
    16381 gcgaagaagc cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg gcagccacgg
    16441 gtatttttcc gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc tggcagatgg
    16501 ccgacgggca gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc gcaggtcgca
    16561 gcctggaaag ccgccagcag gcgggtgaaa tgctgtttga actgattaaa acgcacttcg
    16621 ccgccctgat ggagagccgc ctgctggcgt tgtcgtttga gattgaagag ctgcatccga
    16681 cgctgaattt taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca gattgcccgg
    16741 tggcgctgcg cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc cggataaggc
    16801 gcagccgcat ccggcaatgc cacaggatat cgctatgttc gataaacaca cccacaccct
    16861 gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg cgatctcgct
    16921 ggattacccg gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat gggttcgcct
    16981 gaaaatcgcc gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct cgaaagcgat
    17041 gcaggcaagc tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg acatgttctt
    17101 ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg aagtggagct
    17161 ggctttcgtg ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg acgtctacaa
    17221 cgccacggac tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc acaacatcga
    17281 tccggaaacc cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg ccgccaacgc
    17341 cggggtgatc ctcggcggtc gtccgatcaa accagacgaa ctggatctgc gttggatctc
    17401 cgccctgatg tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg gcgtgctgaa
    17461 tcatccggca aacggcgtgg cctggctggc gaacaaactc gcaccgtatg acgtacaact
    17521 ggaagccggg caaatcattc tcggtggttc gttcacccgc ccggttccgg cgcgtaaggg
    17581 cgacaccttc cacgtcgatt acggcaacat gggttccatt agctgccgct ttgtttaagg
    17641 agagaacgat ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg cagatcggat
    17701 tatggctggg gctgagtagc agctacagcg cagagttact ggccggagca ggattcgact
    17761 ggttgttgat cgacggtgag cacacgccga ataacgtgca aaccgtgctc acccagctac
    17821 aggcgattgc gccctacccc agccagccgg tagtacgtcc gtcgtggaac gatccggtgc
    17881 aaatcaaaca actgctggac gtcggcacac aaaccttgct ggtgccgatg gtacaaaacg
    17941 ctgacgaagc ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt attcgcggtg
    18001 tgggcagtgc gctggcccgc gcctcgcgct ggaatcgtat tcctgattac ctgcaaaaag
    18061 ccaacgatca aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg aagaacttac
    18121 cgcagattct ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg gatctgagcg
    18181 ccgatatggg ttatgccggt aatccgcagc acccggaagt acaggccgcc attgagcagg
    18241 cgatcgtgca gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc aatgagcaac
    18301 tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt gacaccaccc
    18361 tgctcgcccg cgccgccgaa gcgctggcag cacggtttgg tgcgcaggcc accgccgtga
    18421 agcccggcgt gtattaatgc cggatgcgct gcgcttatcc ggcctacact cgcaccaaac
    18481 cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa atcgcggacc
    18541 ggataaggcg ttacgccgca tccgccataa accttgtcgt accctacaaa aatcccatta
    18601 gaggaagaaa aatgagcgac acctcacctg ccataccgga gagtatcgat ccggcgaatc
    18661 agcataaagc gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga
    18721 gcgtctttct gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg
    18781 ccggactcac gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgctacca
    18841 ccctgttcta tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg
    18901 tcggtgcgcg gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctactgcca
    18961 ccatgtttgc cactggcccc accagcttgt acgtactgcg tatactggtt ggcattaccg
    19021 aagccggctt tctgcctggt attctgctgt atttaacctt ctggtttccg gcctatttcc
    19081 gcgcccgtgc caacgccttg tttatggtag caatgccggt aacgacagcg ttgggctcga
    19141 tcgtttccgg ctacattttg tcgctggatg gcgtcatggc attaaaaggc tggcagtggc
    19201 tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg
    19261 atgactcacc ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga
    19321 tgatggataa cgatcgtctg acgctggttc agccagaggg agccatcagc caccacgcca
    19381 tgcaacaacg cagtatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg
    19441 cgtatttctg cctgaccaac acacttagtg cgatcagcat ctggacaccg cagatcctgc
    19501 aaagttttaa tcagggcagc agtaatatca ccatcggcat gctggccgcc gtaccgcaga
    19561 tttgtaccat tctcgggatg gtctactgga gccgtcactc agatcgccgc caggaacgaa
    19621 ggcatcacac cgcccttcct tatttgttcg ctgccgcagg ttggttactg gcttcggcaa
    19681 ctgatcacaa catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg
    19741 caatggcgat tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg
    19801 gtattgcggt gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg
    19861 gctggttgaa agatttgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc
    19921 tggtgattgg tgcggggatt atctgggcga ttccaatgca gtcctcccgt ccgcgagcga
    19981 ccctgtaagg aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt
    20041 acgatgaaag cctgggcacg gacgatgtgc attatcagtc gttcgcccgc atggcggcat
    20101 tttttggccg ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca
    20161 atagcggaca gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt
    20221 ttgtcctgac gccgccgtca
//
LOCUS       DAZPBM010000068        20152 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPBM010000068 DAZPBM010000000
VERSION     DAZPBM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.068"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..632
                     /locus_tag="ACHUZQ_002225"
     CDS             <1..632
                     /locus_tag="ACHUZQ_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1025915.1"
                     /translation="LGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLCMYGLSK
                     DINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVE
                     PGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNKLLELAG
                     KCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTCFFH"
     gene            complement(697..1408)
                     /locus_tag="ACHUZQ_002226"
                     /pseudo
     CDS             complement(697..1408)
                     /locus_tag="ACHUZQ_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(1408..2649)
                     /gene="clsB"
                     /locus_tag="ACHUZQ_002227"
     CDS             complement(1408..2649)
                     /gene="clsB"
                     /locus_tag="ACHUZQ_002227"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1025916.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTRWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2646..3407)
                     /locus_tag="ACHUZQ_002228"
     CDS             complement(2646..3407)
                     /locus_tag="ACHUZQ_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1025917.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3540..3950
                     /locus_tag="ACHUZQ_002229"
     CDS             3540..3950
                     /locus_tag="ACHUZQ_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1025918.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3912..5018)
                     /locus_tag="ACHUZQ_002230"
     CDS             complement(3912..5018)
                     /locus_tag="ACHUZQ_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1025919.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5029..6171)
                     /locus_tag="ACHUZQ_002231"
     CDS             complement(5029..6171)
                     /locus_tag="ACHUZQ_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1025920.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6164..7900)
                     /locus_tag="ACHUZQ_002232"
     CDS             complement(6164..7900)
                     /locus_tag="ACHUZQ_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1025921.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7893..8888)
                     /gene="hlyD"
                     /locus_tag="ACHUZQ_002233"
     CDS             complement(7893..8888)
                     /gene="hlyD"
                     /locus_tag="ACHUZQ_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1025922.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(8891..9562)
                     /gene="cecR"
                     /locus_tag="ACHUZQ_002234"
     CDS             complement(8891..9562)
                     /gene="cecR"
                     /locus_tag="ACHUZQ_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1025923.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9791..11155
                     /gene="rhlE"
                     /locus_tag="ACHUZQ_002235"
     CDS             9791..11155
                     /gene="rhlE"
                     /locus_tag="ACHUZQ_002235"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1025924.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11387..11869)
                     /gene="ybiA"
                     /locus_tag="ACHUZQ_002236"
     CDS             complement(11387..11869)
                     /gene="ybiA"
                     /locus_tag="ACHUZQ_002236"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1025925.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11989..14139
                     /gene="dinG"
                     /locus_tag="ACHUZQ_002237"
     CDS             11989..14139
                     /gene="dinG"
                     /locus_tag="ACHUZQ_002237"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1025926.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14167..15129
                     /gene="ybiB"
                     /locus_tag="ACHUZQ_002238"
     CDS             14167..15129
                     /gene="ybiB"
                     /locus_tag="ACHUZQ_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1025927.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            15270..16355
                     /gene="hcxB"
                     /locus_tag="ACHUZQ_002239"
     CDS             15270..16355
                     /gene="hcxB"
                     /locus_tag="ACHUZQ_002239"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1025928.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            16422..16481
                     /gene="ybiE"
                     /locus_tag="ACHUZQ_002240"
     CDS             16422..16481
                     /gene="ybiE"
                     /locus_tag="ACHUZQ_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1025929.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16584..16844)
                     /gene="ybiJ"
                     /locus_tag="ACHUZQ_002241"
     CDS             complement(16584..16844)
                     /gene="ybiJ"
                     /locus_tag="ACHUZQ_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1025930.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17109..17375)
                     /gene="ybiI"
                     /locus_tag="ACHUZQ_002242"
     CDS             complement(17109..17375)
                     /gene="ybiI"
                     /locus_tag="ACHUZQ_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1025931.1"
                     /translation="MASGWANDDAVNEQINSTIENAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17449..18126)
                     /gene="ybiX"
                     /locus_tag="ACHUZQ_002243"
     CDS             complement(17449..18126)
                     /gene="ybiX"
                     /locus_tag="ACHUZQ_002243"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1025932.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18168..20152)
                     /locus_tag="ACHUZQ_002244"
                     /pseudo
     CDS             complement(18168..20152)
                     /locus_tag="ACHUZQ_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4643 a         5293 c         5471 g         4745 t
ORIGIN      
        1 ctttaggttt ggtggcaacc gcaggactag cctggggagc taagtatctc gaaattacgg
       61 caaccaaata tgattcacca ccaatgtatg tcgctatagg gttattatta ctttgtatgt
      121 atggcttaag taaggatatc aacaagataa atgccgccat cgcgggcgta atatatctgt
      181 ttttactctc tttggtggcg attgtcgttg caagtttagt tcctgtatat gccattatca
      241 tcgtgttcag cactgcgggc gcgatgtttt taatcagtat gctggccggt ttattattta
      301 atgttgagcc tggttctcac cgttttatca ttatgatgac gttgacaggg ttggccctgg
      361 taatcatcgt gaatgcggca ttaatgagtg aacggcccat ttgggtaata agttgcttaa
      421 tgattgtgtt atggccaggc attatctcgc atggacgaaa taagctcctt gaactggcag
      481 gaaaatgcca tagtgaagag ttgtggagtc cggttcgttg cgcttttaca ggtgcattaa
      541 cactctatta ctattttatc ggcttctttg ggatacttgc cgcgatagct ataacgcttg
      601 tctggcaaag gcatacgtgt ttttttcatt agccgtaaat gcacatacta aagaacttaa
      661 ctaaacttca catcgccgct tcatttttcg cccgtagccc agtacaaaga aatagttcac
      721 gctttgacca agtaacagcc agataatcgc ccccattacc atccagttaa cgctggaaat
      781 cagcatctga gcgagggcga atttccatga aggcagcacc agcttttgcc ctttgatggt
      841 catatggcgg tgcttcgcga aagcgcaaaa ccacaaataa accgcgataa tcatcagtaa
      901 gccaatgccg agaatacgca gcgtagtttg atcgacatac cagtgatccg gcaactccac
      961 cacgcctgcg gtaaagataa tccctgccag taaaatatag cccagccagt tggtggtaat
     1021 actgagcgag aaaatccgcg taatagtgct accgggcaac cccagacgag agtacaaacg
     1081 atagcgcata ccaatgccgc cgacccaggt actgagcgtc aggttgaagg cgtagcagat
     1141 aaacgacacc agcatcacct ggcgcttcgc cagtttgtga ccgcagtaaa agcgggcgag
     1201 caggtcatag cagccgtaaa tcagatagct gacgaccacc agcgaccgca ctaagcagcg
     1261 caacgcgatt gtagtcgcgg atgaccttcc agacctcttc ccagtccact tttttggcgt
     1321 agaccaccag caacacaatc accgcgataa aaaacagcca ggtgaggatc ttctttgcta
     1381 agcgccagcg cgggtgtgat ttactcatca gggttttacc cccgtgtttt cagtttctac
     1441 ccgatcctgc gtttccattg tcggttgtgc gggcggatca acctgcgcca gacgtggcgt
     1501 gtgtgccgga agccagccaa ccagcgccgg gaagtggcgt aaaaagtgga acgccagcac
     1561 gcttttggtc aggttccacc tggtgcgttt gggcagcatg gtttcatcca cctgctgaca
     1621 atctgcggcg ataatgccgt tcagattatc gcgcagcgtc tggttaaaat tacgatcgtg
     1681 gatgatgaca tttgcttcga gattcagtga caaactgagc ggatcgagat tactggaccc
     1741 tactgtcgcc cagtgatcgt ccatcaatgc tactttgccg tggagcgggc ggcggcggta
     1801 ctcaaacacc tgaacgccgc ctttaaccag atagttatac agcaagcgcg cgccgactct
     1861 gacaatcggc atatccggtt cgccctgaat gatcagtttg atccgcaccc cgcggcgtgc
     1921 cgctttacgc aaggcgtgta aaaatcgata gccggggaag aagtaggcgt tggcgataat
     1981 gacttcccgc cgcgcctgag tgagcatttt cagatagtgg cgttctatat catcgcggtg
     2041 ttcttcgtta tcgcgccaga ccagcaatac ctgcgcgtct cccggctggc ggttctcttc
     2101 cgctttgtga tgacgtcgcc accagcgtcg tgccgcgctc tgtccaggca ggttttccag
     2161 ctcaaactgg agaatatctt cgacaatcgg cccttcaagg cgtaccgcgt aatcctgttt
     2221 agcctctgga ccgtagctgg acatatgctc ggcggagtaa ttcagcccgc caataaaggc
     2281 gatacgcgcg tcgatcacca caattttgcg atgcatccgg cgaaacacat tggtgcgcat
     2341 accaaaaagg cgagggcggg gatcgtagta gcggaacact acgccagctg ccgtcagttc
     2401 attgacaaac tcatcgctga gatccggcga accgtagcca tccagcaaga cttccgcttt
     2461 aaccccgcgt tgcgctgctg ccagtagtgc cgcatgcagc tgtttgccga cgttatcctc
     2521 aaaccagata aacgtttcaa gaatgatgcg ttcttgtgcc tcgccaatcg ccttaaacac
     2581 cgcgggataa tattgctcgc cgttttccag caactggatc ttattgcctt cgcgccagct
     2641 acatttcata aatgaatctc cgcacttaaa ggggcatgat cagaaaggtg tcgccatgtc
     2701 cgcagcggca acgcggttgg cgcgctggcg ctggcatttt tgacgtagat cctgtccagt
     2761 cgtagtagag gaaattgcac cggaaacgtg cgcgccgggc gtccgtgggc gcgggtaaaa
     2821 atctcatcca gtccggcctg cacttttaac ggatgattag ctttttgccg ccagtcattg
     2881 aaatcacccg ccaccaatac cggttcgccg tccggtagct cattcaccca ttcggcgagc
     2941 atcgcaagct gcgcctgacg gtgcgcctca cgcaggccca gatgtacgca catcacatga
     3001 atcgcttttc cggtcatcgg cggcacaatg cggcagtaga gcacgccgcg cttttccgca
     3061 ccatcgaccg aaacatcgcg attctcataa tgttcaatgg gataacgcga cagtacggcg
     3121 ttgccgtgat gcccttccgg gtatacggca ttgcgaccgt aggcaaaatc gctccacata
     3181 gtgtcggcga gaaactcgta gtgcgaggta tcgggccagt tttccacatg cagcggatga
     3241 acttcgtgcg cgcccatcac ttcctgcagg caaacaatat cggcgctgac ggtacgcacg
     3301 gcatcgcgaa gttccggcaa aatgaagcgt cggttaaacg cggtaaagcc tttgtgaatg
     3361 ttgatggtga gcaccttgaa cgaaaattgt tgtgtttgat cgggcataat tttcctgtct
     3421 ttgcctctta tctcattgaa atagtgtagt cggcgtcaca aaaaggtgcg gtcttacgga
     3481 attttccgta aagttcggta ctctgagtaa gtagagataa attcttcagg agagaagcca
     3541 tgaagtggca acaacgtgtt cgtgtcgcaa cgggtctaag ttgctggcag attatgttgc
     3601 atttactggt agtggcgctg ctggtggtgg gctggatgag taagactctg gttcacgtcg
     3661 gcgtgggatt atgcgcactg tattgtgtca cggtagtgat gatgctggtg tttcagcgcc
     3721 accccgagca acgctggcgt gaggtggcag acgtgctgga agagctgacc acgacctggt
     3781 attttggcgc agcgctgatt gtgctgtggc tgttgtcccg cgtactggaa aacaactttt
     3841 tgctggcaat tgcagggctg gcgatccttg ctggcccggc ggtagtgtct ttgctggcga
     3901 aagataagaa gttacatcac cttacgtcta aacatcgcgt acgccgctga ccctgtcgtg
     3961 gccgttatca ccagtagcgg ccacaaacta ttccacacaa tatccagact cgcatccttc
     4021 aaataaatct gcttggtaat gtccgtaaag tggcgaatag ggttaatcca cgtcaggttt
     4081 tgcagccata ctggcatgtt ttccaccgga gaaacgtaac cggaaaggag aatggcgggc
     4141 atcataaaaa caaacacgcc gataaacgcc tgctgttgtg tcgagcagag tgatgaaatc
     4201 aacagaccga atcccaccag cgataaaccg taaatcacca tcgtaaagta gaacagcgcc
     4261 agcgatccgg cgaaggggat ttgatacgcc cagataccaa tcgccagcac aatggtggcc
     4321 tggaaggtgg cgacaattaa cgccggtacg gctttgccga tgaagatctg ccaggtggtg
     4381 agcggcgaaa ccagtagctg atcgagcgta ccttgttcac gttcgcgggc gacggaaagt
     4441 gaagtgacga tcattacgcc gatagtggtg atcatggcga tcagcgacgg caccacaaac
     4501 catttgtagt caagattcgg gttataccag ttgcgtacca ccagctcgct gttgttaggt
     4561 ttcggttttc cttccagcag ctcctgctga taatttttga cgatctgttg caggtagttg
     4621 gcggcaattt gcgcactgtt ggagttacgc ccgtcgagga tcaactgcaa cggcgcggtc
     4681 tggaaggtat ccagtttgcg cgagaagtca gccgggaaac gcaccagtag taacgccttt
     4741 tgtgtgtcga tggttgggcg gatctcctgt gggcttttca gcagcagcac atgggtaaag
     4801 gcgctggcgc gggcaaaacg ttgggtcagc tccaccgaat gctcgccgtt atcttcatcg
     4861 tagatggcga tggtggcgtt agtcacttcc agcgtggcgg cgaacgggaa caggatcacc
     4921 tgaataagca caggtaaaat cagaatcgcg cgggtttgtg gttcgcgcag caacgactgc
     4981 aactctttgc ggattaacgt ccataagcga tgaaacatgc tcttctccct aatccagccg
     5041 acgtttggtt ttcagccacg tcaggccgat aaacatcacc gccgaagcga tcaaaaacag
     5101 cacgtttacc accagcacca ctggaatatt cccggcgagg aacaggcttt gcagggtgct
     5161 gacgaaataa cgagcgggaa taatgtacgt caccgcgcgg atcaccgcgg gcatactgtc
     5221 gatctgaaaa ataaagccgg aaagcataat cgacggcagg aacgcggcgt tcagggcgac
     5281 ctgagcggca ttgaactggt tgcgggtaat cgtggaaatc agcagcccca tccccagggt
     5341 actgagtaaa aacaggctgg agataaaaaa cagaataagc agcgaaccgc gatacggcac
     5401 gccgagaata aacactgaca ccagcataca cagcagcatc gccagcatcc cgagaaagta
     5461 ataagggatc agcttacaca gcagcagttc cgtgcgggta atctccgtag agagcagagc
     5521 ctccatggtg ccgcgttccc attctcgcgc caccaccagc gaggtgagaa tcgcgccgat
     5581 gaccgtcatg atgatggtca ctgcaccggg gataatgaag tgctggctaa tcgccgccgg
     5641 gttaaaccag tagcgggttt gtacatcaat aagcggttca aaagtctgcc cgttgtcctc
     5701 cgctcgctgc atttgccaga tctgccagat ctgccagatc ccttcgacat acccctgtac
     5761 aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg cggtggcgtt
     5821 ggcgcgctcc atctgttccg caaaatccac cggaataacc accagaccgc gaattttccc
     5881 cgcctgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt cgatgtaggg
     5941 cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt ccagcaagat
     6001 cccgacccgc agcttgctgg agtcgaggtt aatgccataa ccaaaaataa acagcagtag
     6061 cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct gccgcgtctc
     6121 tttgacgcac agcgcccgca cgcgacgcca ggaaaggatc gggttactca ttgctatgct
     6181 ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga tcgggttgct
     6241 catcgttagc cgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt aatttcccac
     6301 ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg gtggtgacca
     6361 tcaccgtgac gcctttctct accatgctgt tgatatgtag ccaaaattca cggcgggtga
     6421 ggggatcaac gccggacgtc ggttcgtcga gaaacagaat gtccggttca tgcatcagcg
     6481 aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg gcgtgggagg
     6541 cgatactttt caggccgaac gcctcgctca tgcgggagat tttttcgttc tgcgcccgac
     6601 cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc aggttaccgt
     6661 agagcgaaaa cttttgcgcc atatagccga gatgctggcg cgctttacca gaactctctt
     6721 tcagatccat ccccagtacc agcgcctgac cagaggtcgg caccagcaaa ccgcacatca
     6781 tcttaaaggt ggtcgatttg cccgcgccgt ttggccccag caaaccaaaa atctccccgc
     6841 gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc ttggtcagtt
     6901 ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgtaat attgcgccca
     6961 gcggcgattc cgaggttccg gcaccgccca gcaaatcaat aaacgcatct tcaaaacgcg
     7021 gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata tcgtctggtg
     7081 tggtctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg tcgctgacct
     7141 gcggcagttt caatgcccgt tgcaacagtt tgcggttgcc ctcgtgtgga cttgtcatca
     7201 gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttccccctga tacagcaact
     7261 cgccttcatt catcagtaac acgtcacggc actgctcggc ttcgtcgaga tacgaggtac
     7321 tccagaggat taacatccct tcgcctgcca gctcatgcac catctgccac agttcgcgcc
     7381 gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc ggttcgccca
     7441 ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg cccgccaggc
     7501 gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt tgcttacgtg
     7561 cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc accgtgagat
     7621 cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc gcgccgtcgt
     7681 ttttgattgg atcaaagcca atcaccgtgg cattgccgct gtcgggtttc agtaatcccg
     7741 ccagcatccg cattagcgtg gttttacctg caccatccgg ccccaccagc cccgtcacat
     7801 aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg cccggaaagc
     7861 gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc gttaccgaat
     7921 tgcaccgtca ccggcattcc ctggcgtaac gcatcatcgg cgtcggtcac cacaatacgc
     7981 aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg ggtaaattca
     8041 gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg accatcggta
     8101 taaagcagca ctttgcgccc cggctgggcc tggtcaagat accgttcatc aacataagcg
     8161 cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc attgaggacc
     8221 gtgcctggct ccaccgcgcg cgttaacagc gtgccatcag acggggctat caacgttgag
     8281 tcctgtaaat tcaactccgc ctgcgccagt tgcgcctgcg cctgttcgag gctggctttc
     8341 gcctgggcga tgtcctgttc acggttaccg gaacggtact gacgcaattt atcctgtgct
     8401 gatttcagcg ttgcctgcgc ctggtcgcgc gaggagcggg cattttccag gtcatttgcc
     8461 gaaatagtgc ggcttttcca cagtccttgc tggcggttat agaagttctg cgcatagtca
     8521 taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc atcgcgatac
     8581 ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt cgcctgcatc
     8641 agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc tttgatagca
     8701 tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact aagattcacc
     8761 gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct ttgataccac
     8821 cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagttcgat cacgacaggt
     8881 tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg atatgacagg
     8941 tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag ccggtacgta
     9001 acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac gcatgggtat
     9061 gaaggatcat gcgggtgtca ttggcgtcgc tgccggtcca ggcggcaatc aggcgtgtca
     9121 ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct gccgtgggag
     9181 agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg gtgagcagct
     9241 taatcatgtt cctgcaggcg cgaaggagca gttcacggat ggcagcccga tcaggctgtg
     9301 gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg ccaataaaat
     9361 cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag tagtaggtga
     9421 tggcagcgat attctgcccg gcttgggcgg ctatctcgcg ggtggtggcg ttcattccat
     9481 attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt tcacccttga
     9541 ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt gattaagatt
     9601 atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc agtcacaggg
     9661 ataatttatg cgctgcgtca caaaaactgc tacactccgc tcgctcatga cattgtggtt
     9721 tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt cggggcggtt
     9781 cggagtagtc atgtctttcg attatttggg tttaagccct gatatcctgc gcgccgttgc
     9841 cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg cggtgctgga
     9901 aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg gctttacgct
     9961 gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc gtccggtacg
    10021 tgcgctcatt cttaccccga cccgtgaact ggcggcgcag attggcgaaa acgtccgtga
    10081 ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggtggtgtga gtattaaccc
    10141 gcagatgatg aaactgcgtg gcggcgttga tgtgctggtg gcaacgccgg gacgtttgct
    10201 ggacctggaa catcagaatg cagtgaagct ggatcaggtt gaaatcctcg tcctcgatga
    10261 agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt taacaaaact
    10321 acctgcgaag cgccagaacc tgttattctc cgcgaccttc tctgacgata ttaaagccct
    10381 ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca ataccgcgtc
    10441 tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg aattgctgtc
    10501 gcacatgatt gggaaaggga actggcagca ggtgctggtg tttacccgta ccaaacacgg
    10561 cgctaaccat ctggctgaac agctcaataa agatggcatc cgtagtgcgg cgatccacgg
    10621 caataaatcg caaggtgcgc gtactcgtgc gctggctgat tttaaatcgg gcgatattcg
    10681 tgtactggtg gcaactgaca tcgctgcgcg cggcctggat attgaagagc tgccgcacgt
    10741 cgtcaactat gaactgccaa acgtaccgga agattatgtc catcgtatcg ggcgtaccgg
    10801 tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac acaaactgct
    10861 gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc cgggctatga
    10921 gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac gtggcggcgg
    10981 cggtcgtggg caaggtggtg gtcgcggtca acagcaacca cgccgtgggg aaggtggcgc
    11041 aaaatctgca agcgcgaaac ctgcagaaaa accgtctcgc cgcctcggcg atgccaaacc
    11101 ggcaggcgaa caacaacgtc gccgccgtcc gcgtaaacct gccgctgcgc agtaatcttt
    11161 tatgccgggc tatgcccggc atcaggctga tgaacaaacg caaaactgcc tgatgcgcta
    11221 cgcttatcag gcctacgtga actctgcaat atattgaatt tgcatgcttt tgtaggccgg
    11281 ataaggcgtt tacgccgcat ccggcatttc acaacaagca cttgtcagca atttgagaac
    11341 acgggaaaaa atttatctgt tttaccgccc ataagccacc gagacgttac ttctctatag
    11401 ccaattgttc tcgcaactcc attaaaaggt agcccagtcg attcttgccc ttaccattac
    11461 caccgtctcc ccagtaagca tcgttttccg tatgctcaac cagttttgcg ggcgcggttg
    11521 ccagcaagag cacacgcagt tctgcatgct gttcgaattt agcacgaagt gctttacgca
    11581 tcacctgttc tttaaccgac tcccagtttt tacgaagtgg cttggaacga ttacgcccca
    11641 tgcgagcggc gaccatgggg gaagaaaccc gacggatctc ttcgcggtat ttttcatcaa
    11701 ggaatttttg tgcctgaaaa tagtgttcgg aggtgggcca ggttttcccg tcaaccttga
    11761 tgggccaggc ggcaaagtta gaaaaatcac cgtagtcatc gctggtgctg tagaaattta
    11821 tgatggtgtc ttgcatgacg tgttggatcc tttgtgctcg aacgggcatt aaaccgcatt
    11881 atgttggtgg ttattgcgag ccgctttcca gaaacagaaa aaccattacc cctgaaaacc
    11941 gaaaaatgcc acaatattgg ctgtttatac agtatttcag gttttctcat ggcattaacc
    12001 gccgcgctta aagcgcaaat tgccgcctgg tataaggcgc ttcaggaaca gatccccgac
    12061 tttattcccc gtgtgccgca gcggcagatg attgcggacg tcgccaaaac gctggccgga
    12121 gaagaagggc ggcatctggc gattgaagcc cccaccggcg ttgggaaaac gctctcctat
    12181 ttgatccccg gcatcgccat tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc
    12241 aacgtggcgt tgcaggatca gatttacagc aaagatttac cgctgctgaa aaagatcatt
    12301 cccgatctta aattcactgc cgctttcggg cgtgggcgct acgtttgtcc gcgtaatctg
    12361 acggcgctcg ccagcactga acccacgcaa caggatctgc tggcgtttct tgacgacgaa
    12421 ctgacggcaa ataatcagga agagcaaaaa cgttgtgcga agctgaaggg cgatctcgac
    12481 acttatagat gggatggtct gcgcgatcat acggatatcg ctatagatga tgatctctgg
    12541 cgtcgtttga gtaccgacaa agccagctgc ctcaaccgta actgttacta ctatcgtgaa
    12601 tgcccgtttt ttgttgctcg tcgggagatt caggaagcgg aagtggtggt ggcaaaccat
    12661 gcgctggtga tggcggcgat ggaaagcgaa gccgtactgc ctgacccgaa aaatctgctg
    12721 ctggtgctgg acgaaggtca tcacctgcca gatgtggccc gggatgcgct ggagatgagc
    12781 gctgaaatca ccgcgccctg gtatcgacta cagctggact tgttcacgaa actggtcgct
    12841 acctgcatgg agcagtttcg cccgaagacc atcccaccgc tggcgatccc tgaacgtttg
    12901 aatgcgcatt gtgaagagtt gtatgagctt atcgcctcgt taaacaacat tctcaatctc
    12961 tacatgcctg ccgggcagga ggcagagcac cgttttgcga tgggcgaact gcctgatgaa
    13021 gtgctggaga tctgccagcg gctggcaaaa ctcaccgaga tgctgcgtgg tctggcggag
    13081 ttatttctta acgatttaag tgagaaaacc ggcagccatg acattgtacg cctgcatcgg
    13141 ttgattttgc agatgaaccg cgcgttgggg atgttcgagg cgcaaagcaa actctggcgg
    13201 ctggcgtctc tggcgcaatc ttccggtgcg ccggtgacca aatgggcgac gcgggaagag
    13261 cgcgaagggc aactgcatct ctggtttcac tgcgtgggga tacgtgtcag cgatcagctg
    13321 gaaaggctgc tgtggcgcag tattccgcac attattgtca cctccgcaac cttgcgttcg
    13381 ctgaatagtt tttcgcgttt gcaggagatg agtggtctga aagagaaagc gggcgaccgt
    13441 tttgtggcgc tggattcccc ctttaaccac tgcgaacagg gcaaaattgt tattccccgg
    13501 atgcgcgttg agccttccat cgacaacgaa gagcagcata ttgccgaaat ggcggccttt
    13561 ttccgtaagc aggtggagag caaaaaacat ctcggtatgt tggtactgtt tgccagcgga
    13621 cgggcgatgc agcgctttct cgactatgtg acggatttac gtctgatgtt gctggttcag
    13681 ggcgatcagc cgcgttaccg tttagttgaa ctgcaccgca aacgcgtcgc caacggtgag
    13741 cgcagcgtgc tggtgggctt acagtcattt gccgaagggc ttgatttgaa aggtgatctg
    13801 ctcagccagg tgcatatcca caaaatcgct tttccgccca tcgacagccc cgtggtgatc
    13861 accgaagggg aatggctgaa aagcctcaac cgctatccgt ttgaggtgca aagcctgccg
    13921 agcgcctcgt ttaacctgat tcagcaggtt gggcgactga ttcgaagcca cggttgctgg
    13981 ggcgaagtgg ttatctacga caaacgcttg ctgaccaaaa attatggcaa gcgactactg
    14041 gatgcattac cggtatttcc gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag
    14101 aaagaaaaaa cgaaatcccc acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag
    14161 tcgtaaatgg actatcgcaa aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc
    14221 gatttagacc gggatactgc gcgcggtctg tatgctcata tgctcaatgg tgaagtccct
    14281 gaccttgagt tgggcggcgt attgattgcg ctgcgtatca aaggagaagg ggaagcagag
    14341 atgctcggct tttacgaagc catgcaaaat cacaccatca agctgacgcc gccagcaggc
    14401 aagccgatgc cgattgtcat ccccagttac aacggcgcgc gtaaacaggc caacctgacg
    14461 ccgttgttgg cgattctcct gcacaaactc ggttttcctg tggtggttca cggggttagc
    14521 gaagatccaa cccgcgtgct gactgaaacc atttttgaat tgatgggtat tacgccaacg
    14581 ttgcacggcg ggcaggcgca ggcgaagctc gacgaacatc aaccggtgtt tatgccagtc
    14641 ggcgcgtttt gcccgccgct ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt
    14701 aacagtgcac ataccctggc gaaactggcg acgccatttg ccgaaggtgc ggcgctgcgt
    14761 ctttccagcg tttcgcatcc ggaatatatt ggacgcgtcg cgaagttctt tagcgatatc
    14821 ggcgggcggg cgctactaat gcatggtact gaaggtgaag tgtatgctaa tccgcagcgc
    14881 tgcccgcaga tcaatctcat tgaccgtgaa gggatgcggg tgctgtatga aaaacaggac
    14941 actgctggta gcgagttact gccacaagca aaagatccgg aaaccacggc gcagtggatt
    15001 gagcgttgcc ttgctggcag cgaaccgatt cccgaatcgc tgaaaatcca gatggtttgc
    15061 tgcctggtgg ctatgggtga agcggcaact atcagcgacg gcctggcgcg cgttaatcag
    15121 gcattttaat tcttttctca gccggatgag ccatgtcatc cggcttttac cccaccgcaa
    15181 tatgaaattc ctgcatcttt attgaccttc ccacgcccgg cgtgcagcat aaaaatacaa
    15241 caaacacata acataaacag gagttaacca tggaaagtgg tcatcgcttt gatgctcaga
    15301 cgctgcacag ttttattcag gctgtatttc gtcagatggg tagcgaggaa caagaagcga
    15361 aattagttgc cgatcattta atcgcggcaa acctggcagg gcatgattca catggtattg
    15421 gcatgatccc aagctatgta cgctcctgga gtcaggggca cctgcaaatt aaccatcatg
    15481 ccaaaaccgt taaagaggcg ggggcggcgg tcacgctcga tggcgatcgc gcatttggtc
    15541 aggtcgcggc acatgaagcg atggcgctgg ggattgagaa agcgcatcag cacggtattg
    15601 ccgccgtggc gctacataac tcgcatcata tcggccgtat cggttactgg gcggagcagt
    15661 gtgcagcggc ggggtttgtc tctatccact ttgttagcgt ggtcggtatt ccaatggtcg
    15721 cgccgttcca cggtcgcgac agccgctttg gcaccaatcc gttctgtgtg gttttccctc
    15781 gtaaagataa tttcccgctg ttgcttgatt acgccaccag cgccattgca tttggcaaaa
    15841 cccgcgtcgc ctggcataaa ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg
    15901 gcgtgccgac gaccaatccg gcggtaatgc aggagtcgcc gttgggttcg ctgttgacct
    15961 ttgccgaaca taaaggctac gcccttgcag cgatgtgtga aattcttggc ggggcgcttt
    16021 ccggcggtaa aacgacgcat caggaaacgt tacaaaccag tcccgatgcc attcttaact
    16081 gcatgaccac tatcatcatc aacccggaac tcttcggcgc gccggattgt aacgcgcaga
    16141 ccgaagcctt tgccgagtgg gtgaaagcct cgccacatga tgatgataag ccgattttgc
    16201 taccgggcga gtgggaagtg aacacgcgtc gcgaacggca gaagcagggg attccactgg
    16261 atgcgggaag ctggcaggcc atttgtgatg cagcgcggca gattggtatg ccggaagaga
    16321 cgttgcaggc tttctgtcag cagttagcca gctaaaaaaa agcccgtcca gtggcggacg
    16381 ggcaaacaag ggtaacatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca
    16441 gacagggaaa tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcggat
    16501 tttgtaggcc tgataagacg cggtagcgtc gcatcaggca tctgttgccg gatgcggcgt
    16561 aaacgcctta tccgtcctac gaattactta taaattaccg cagtaccgct taatttattg
    16621 ttgttggtgg cggaagtgat gctgtatcca ctggcaccgg ctgccgcggc tttctcagcc
    16681 agtttcgctt ccagggcatc gagggtggat gcgccatcgg cagaaaccac gccgatttta
    16741 ttcatgttct gtgcctggga tgccgttatc ggttccgccg cgaacacgcc aaatgacaga
    16801 gttgaaagag ccatagcagc aacaacagta ttgatagttt tcataattaa tctctcgcag
    16861 gtgatttttg tataagtgac gttgtttcgt cgatgtgatg agtatcacgt ttttttacga
    16921 gagataaaat cgaagagaat tgaccgcctt gttcaaataa attgattgat atctaacata
    16981 ctgataaacc atttatttgt tgtacttatg aacttgtttt ataccgcgtg gcagtcacag
    17041 cagcgttaaa ggcacggaat gccacgcaaa ataaaaatta tactcgctat gcatctcgcc
    17101 agttgtagtt aacgtaactg gctgtctttc gaacctctgc gattatatcc tgtataagct
    17161 ggtttttgta aatctttctc ctgctgacaa tgaatacata agcgcacgcc aggaatggct
    17221 tcccgacggg cctgcgggat gggggcaccg cactcttcac attcatccag gctttcgccg
    17281 cgcggaattt caccccgagc gcgggcaatc gcattttcaa ttgtactgtt gatctgttcg
    17341 ttgacggcgt catcgttagc ccaaccggat gccatatcga cctccccata tcaatacttg
    17401 tacagttaag tgtagctaat ccagggacga actcgggcag ttcaagcatc agatctccga
    17461 ccattcccgc agcagattat gataaagatt aagcagcgac aggatctctt cactttcgcc
    17521 gtagcggctt ttcagcgact gaatattgtt gtccagttca aacagcatgg cgcgcttttt
    17581 atcatcgcgg atcatcgact ggatccacat aaatgatgcc actcgtacgc cgcgggttac
    17641 gggtggcacg caatgctggc tgctggaggg atacaacacg agatcgcctg ccgggagttt
    17701 tacccgatgt tgtccgaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg
    17761 atcgcttaaa aacagcgtgg cagaaaggtc agtacgcatc cagccgtttt gtggatggct
    17821 gcgtactgcg ccatccacat ggaaaccata ggtttcattg ttctgatagc gattaaacag
    17881 cggcgtggaa agggtacgcg gcaaggccgc ggcaaagaac aaagcatgtt ggttaaccgc
    17941 gttcagcacc tcattttgca gggcggcgta taacgtgctg cgggtgtcga cctgttgatt
    18001 gttcttaact tgcgcacctt gtgcgccggt ggtgacgcgt ccatccaccc attcggcttg
    18061 ttccagttgt tcgcgaaacc gggcgacgtc ctgtggcgat aacacgccgg gaatgtggta
    18121 catcatacaa gttctccaaa aagtggggcc tgcgccccac atctgaatca gaaatgcata
    18181 ttggctgtga gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttgttg
    18241 attgaggcga cgtaatcagt atcaaacagg ttgtagacgt ttagctggaa gtcgagattg
    18301 cgattaaccc gataccccag tttggcatcg gcgacccagt aaccttcggt aaatgctggc
    18361 gttcccaccg cgccgtctga acctttatgc atactgccga tatagcgtgc gcccgcgcca
    18421 acagagatat cgtcggttgc ctgatattgg ctccataagg tgaaggcgtg ctccggggta
    18481 tacggcagcg atgaggaacc atcctgggca acatctttgc cgtttttgat ggttgctttt
    18541 tgctgggtat agccgccaat cacctgccac gcgggagtga tattcccggc cacggatatc
    18601 tcatagcctt cgacgcgttt cttaccgtat tgcgagtaag ttccgtcatc attttgctca
    18661 acttcatttt cgatatcagt acggaacagc gcggcggtga gcaacagacg tttatccaga
    18721 acctgccatt tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg
    18781 ttggcactgt taccgctgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc
    18841 tgggaaacgg catagttaat atagacattg ccgttttccg tcaggtgata cagcgccccg
    18901 gctttccagt tcatcagatt gcccgacttg gcggtgtcga cggtggtgac cggagaacct
    18961 tttgccacac cagttgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca
    19021 ctgtcatatt cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg
    19081 gtgatttgca gcgtatcaaa ggcgtaaatt gcgaaggtat ccgtctgacc attggcgttt
    19141 gcgccgttgc gcgtcaggcc gccgggatga atgctgctgt caggatgata aatatttacc
    19201 gcgggtaacg tcaccggatt aacgccgtag ttagtctgcg tttcacgggt aaattccacg
    19261 ccggtactga catcatgacc gatagaaccg gtatagaacg tcgaggtcag gttggtctgg
    19321 ttggtgagaa ttttattact cacatctttg gtattcgccg tgcgtgacca ggttcagcta
    19381 ttcacatcgc tggtgggctg agtaatattc gacgccccgc ccataatcgc cgtcatcagg
    19441 taatcctgct ttacgcgcga ccaacgggta gtattgcgaa tggtggtgtt atcgttgatg
    19501 tcgtgctcaa aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg
    19561 ccgtaaaagt tatgagtatc aacttttccg gaatgattca gggccgccgt tcccgcagat
    19621 ggggcagaat agcccggcaa accgatggtc ggaatgccgc cgtctggcgt gttgtgctgg
    19681 gtgacatgca gataattaag atacaaacga ttcgctgtac caaggccaaa agcgacagaa
    19741 ggggcgacgc cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg cgttttttcg
    19801 cccattacat tcaggcgcac cgcggtggta tcaccaatga cctgattgac gtccagcgtg
    19861 ccgcggcgga accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc
    19921 ggctgtttgc tgatcatatt gatcgagccc gtcggtgcgc tgcgcccgta gtcggtgccg
    19981 gacggccctt taatcacttc gacctgctcg gtattgaagg tgtcgcgcga gacgctgccg
    20041 atatcgcgaa tgccatcaat ataaatactg ttagaggtat cggcaccacg catataaatg
    20101 gcgtcgccag tggtggagtt accgttctca cccgcaaaaa acgcacccac gc
//
LOCUS       DAZPBM010000069        20106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPBM010000069 DAZPBM010000000
VERSION     DAZPBM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.069"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            179..850
                     /locus_tag="ACHUZQ_002245"
     CDS             179..850
                     /locus_tag="ACHUZQ_002245"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR1025933.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            860..2056
                     /locus_tag="ACHUZQ_002246"
     CDS             860..2056
                     /locus_tag="ACHUZQ_002246"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1025934.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2056..2616
                     /locus_tag="ACHUZQ_002247"
     CDS             2056..2616
                     /locus_tag="ACHUZQ_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1025935.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2618..3391
                     /locus_tag="ACHUZQ_002248"
     CDS             2618..3391
                     /locus_tag="ACHUZQ_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR1025936.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3579..3854
                     /gene="frmR"
                     /locus_tag="ACHUZQ_002249"
     CDS             3579..3854
                     /gene="frmR"
                     /locus_tag="ACHUZQ_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR1025937.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3889..4998
                     /gene="frmA"
                     /locus_tag="ACHUZQ_002250"
     CDS             3889..4998
                     /gene="frmA"
                     /locus_tag="ACHUZQ_002250"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR1025938.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5091..5924
                     /gene="fghA"
                     /locus_tag="ACHUZQ_002251"
     CDS             5091..5924
                     /gene="fghA"
                     /locus_tag="ACHUZQ_002251"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1025939.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6150..6689)
                     /locus_tag="ACHUZQ_002252"
     CDS             complement(6150..6689)
                     /locus_tag="ACHUZQ_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR1025940.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6791..8002)
                     /gene="mhpT"
                     /locus_tag="ACHUZQ_002253"
     CDS             complement(6791..8002)
                     /gene="mhpT"
                     /locus_tag="ACHUZQ_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR1025941.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8289..9302)
                     /gene="mhpE"
                     /locus_tag="ACHUZQ_002254"
     CDS             complement(8289..9302)
                     /gene="mhpE"
                     /locus_tag="ACHUZQ_002254"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR1025942.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9299..10249)
                     /gene="mhpF"
                     /locus_tag="ACHUZQ_002255"
     CDS             complement(9299..10249)
                     /gene="mhpF"
                     /locus_tag="ACHUZQ_002255"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR1025943.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10246..11055)
                     /gene="mhpD"
                     /locus_tag="ACHUZQ_002256"
     CDS             complement(10246..11055)
                     /gene="mhpD"
                     /locus_tag="ACHUZQ_002256"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR1025944.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11065..11931)
                     /gene="mhpC"
                     /locus_tag="ACHUZQ_002257"
     CDS             complement(11065..11931)
                     /gene="mhpC"
                     /locus_tag="ACHUZQ_002257"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR1025945.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11949..12893)
                     /gene="mhpB"
                     /locus_tag="ACHUZQ_002258"
     CDS             complement(11949..12893)
                     /gene="mhpB"
                     /locus_tag="ACHUZQ_002258"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR1025946.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12895..14559)
                     /locus_tag="ACHUZQ_002259"
     CDS             complement(12895..14559)
                     /locus_tag="ACHUZQ_002259"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR1025947.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14601..15434
                     /locus_tag="ACHUZQ_002260"
                     /pseudo
     CDS             14601..15434
                     /locus_tag="ACHUZQ_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15511..16593
                     /gene="lacI"
                     /locus_tag="ACHUZQ_002261"
     CDS             15511..16593
                     /gene="lacI"
                     /locus_tag="ACHUZQ_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR1025948.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16716..19790
                     /gene="lacZ"
                     /locus_tag="ACHUZQ_002262"
     CDS             16716..19790
                     /gene="lacZ"
                     /locus_tag="ACHUZQ_002262"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR1025949.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            19842..>20106
                     /locus_tag="ACHUZQ_002263"
     CDS             19842..>20106
                     /locus_tag="ACHUZQ_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1025950.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         4724 a         5512 c         5224 g         4646 t
ORIGIN      
        1 ttaacatatg aattgcataa aaacattacc cagtcgaacc tccgggtctg aaagcccttg
       61 cttttcagtt attagcaaca tttaacattt ataaattaac atctggctgc gccagagatt
      121 acgtctctga gtgcatcgtt gtgcttgtag tataataaat aaaatggagt gtcattaagt
      181 ggataaggtt ttagattcag ccctcctttc ttcggcaaat aaaagaaagg gtatccttgc
      241 tattggcgca catcctgacg atatagaatt aggctgtggc gcatcgcttg ctcgtcttgc
      301 gcaaaaagga atttatatcg cagcagtggt gatgactacc ggcaactctg gtacagatgg
      361 aataatcgat cgccatgaag aatcgcgcaa cgccctaaag atattagggt gccaccaaac
      421 tattcatctt aattttgctg acacccgcgc tcatttacag ctcaatgata tgatttccgc
      481 cctggaagac atcattaaaa atcaaattcc ttctgatgtt gaaatcatgc gggtatatac
      541 catgcatgat gccgaccgcc atcaggatca tctcgctgtt tatcaagctt caatggttgc
      601 ctgccgcact attccacaaa ttctcggcta cgaaacccca agtacctggc tttcatttat
      661 gcctcaggtt tttgaatccg ttaaagaaga atatttcaca gtcaaacttg ctgcattaaa
      721 aaaacataaa agccaggaac gacgtgatta tatgcgtcat gatcgcctgc gtgcagttgc
      781 acaatttcgc gggcaacagg tcaatagcga tctgggtgaa ggctttgtaa ttcataaaat
      841 gattctttga gcaaattgca tgaaaacctg gatatttatc tgtatgtcca tagcaatgtt
      901 gctatggttt ttaagtacgc taagacgtaa acccagtcaa aagaaaggct gtattgacgc
      961 cattatacct gcgtataacg aaggcccgtg tctggcgcag tcactggata atctactgcg
     1021 taacccttat ttttgccggg taatttgcgt taacgacggc tccacggaca ataccgaagc
     1081 ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt gttgccgtca cgcaaaaaaa
     1141 taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat gccacctgcg accaggtttt
     1201 tttaagtgat gccgacacct atgttccgcc cgatcaagac ggaatgggct atatgctggc
     1261 agaaattaag cgcggtgctg atgccgtagg cggcattccc tctactgcgt tgaaaggcgc
     1321 ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg atgattgtta tgaagcgcac
     1381 gctacagcag ctcctgggcg gcgcaccgtt tattatcagc ggtgcctgcg gaatgttccg
     1441 tactgatgta ttgcgtaagt tcggtttctc ggatcgtact aaagtcgaag accttgatct
     1501 cacctggaca ttagtggcaa acggctaccg tattcggcag gcgaatcgct gcatcgtata
     1561 cccacaggaa tgcaacagtc cgcgtgagga gtggcgtcgc tggcggcgtt ggattgtggg
     1621 ctacgcggtc tgtatgcgcc tgcataaaag gcttttattt agccgcttcg gtatcttcag
     1681 tatatttcct atgctgttgg ttgtgattta tggcgttggg atttatctca ctacctggtt
     1741 taatgaattc atcaccaccg ggccgcatgg tgtggtgttg gcaatgtttc cgcttatctg
     1801 gatcggcgta gtttgtgtta ttggtgcttt tagcgcctgg tttcatcgtt gctggttgtt
     1861 ggtgccttta gcgccgcttt ccgttgtgta tgtattatta gcttatgcca tctggattat
     1921 ctatggactt attgcctttt ttactggacg cgaacctcag cgcgacaaac ccacccgcta
     1981 ttccgcactg gtggaagcgt caaccgctta ttcccaacct tctgtcacag gaactgaaaa
     2041 actatctgaa gcttaatgtt aaagagaaaa attattcaga ttgcagacca ggtaattatt
     2101 gatgaaactg caggtgaagt tgttatcggc gcgaataccc gtatttgtca tggtgccgtt
     2161 attcagggtc cggtagtgat tggcgcaaac tgcctgatag gtaattatgc gtttattcgt
     2221 cctggcacaa taatcagcaa tggtgtaaaa attggttttg ccaccgaaat taaaaatgcg
     2281 gttattgaag cggaagcaac gattggtccg caatgttata ttgccgactc ggtagttgca
     2341 aaccaggcat atttgggcgc acaagtacgt accagtaatc atcgtctgga tgaacagcct
     2401 gtgtctgttc gaactccaga gggaattatc gctaccggat gcgataaatt aggttgttat
     2461 atcgggcagc gttcacgcct tggcgtacaa gttattattt tgcctgggcg aattatttcc
     2521 ccgaacacgc aacttggccc gcgcgtgatt gtagaacgta atttacctac cggaacttat
     2581 tcactccgac aagaacttat ccgtacagga gattaaaatg ataaaacgga cgttattagc
     2641 ggcggccatt tttagcgcat tgcccgctta tgccgggtta acttccatta ccgcgggcta
     2701 cgattttacc gattattctg gcgatcatgg caaccgtaat ttagcgtatg ctgaactggt
     2761 ggcgaaagtt gaaaacgcaa cgctgctttt taatctttcc caggggcgtc gtgattatga
     2821 aactgaacat ttcaatgcca ctcgcggtca gggtgcggtc tggtataaat ggaataactg
     2881 gctgacaacc cgaacgggta ttgcctttgc ggataatacg ccggtctttg cccgccagga
     2941 ttttcgtcag gatattaacc tggccctgtt gccaaaaacg cttttcacga ccggttatcg
     3001 ctacactaaa tattacgatg atgtcgaagt cgatgcctgg caaggcggcg tatcactcta
     3061 tactggcccg gtgatcacca gctaccgcta tacccattat gactccagcg atgcaggtgg
     3121 tagttatagc aatatgattt ccgtgcgtct gaatgacccg cgcggcactg gttatacgca
     3181 actatggcta agccgcggaa caggcgctta cacctatgac tggacgccag aaacacgcta
     3241 cggcagcatg aagagcgtca gtttgcaacg tattcaaccg ctgactgagc aacttaatct
     3301 cgggctgacg gcaggtaaag tgtggtacga caccccaacc gatgattata acggtctgca
     3361 acttgcagcc catctgacct ggaaattctg attgcttctg ccgcccgcta tccggggcgg
     3421 ccttccctgc cgattagccc cactcccttt cctcgttgtt ttccgaccac attcaccgga
     3481 taaattttat tctccagcgt tatatactat agggaggtat gcattgacat atagcatacc
     3541 tccctatagt atattgcgtg cagatgatga ggtgcgaaat gcccagtact ccggaagaga
     3601 agaaaaaggt ccttactcga gttcgtcgta ttcgggggca gattgatgct ctggaacggt
     3661 cgctggaggg tgatgccgaa tgccgtgcca tactccaaca gatcgctgcc gttcggggcg
     3721 cggctaacgg gctgatggca gaagtgcttg aaagccatat ccgggaaacg tttgaccgaa
     3781 atgactgcta cagccgcgaa gtcagccaat ccgttgacga cactattgaa ctggttcgag
     3841 cctatcttaa atagctgaat ctattaccat attgaggaag agcgagagat gaaatcacgt
     3901 gctgccgttg catttgctcc cggtaaaccg ctggaaatcg ttgaaattga cgttgcacca
     3961 ccgaaaaaag gtgaagtgct gattaaagtc acccataccg gcgtttgcca taccgacgca
     4021 tttaccctct ccggggatga cccggaaggt gtattcccgg tggttctcgg tcacgaaggg
     4081 gccggcgttg tggttgaagt cggtgaaggc gtaaccagcg tcaaacctgg cgaccatgtg
     4141 atcccgcttt acaccgcgga gtgcggcgag tgtgagttct gtcgttctgg caaaactaac
     4201 ctctgtgttg cggttcgcga aacccagggt aaaggcttga tgccagacgg caccacccgt
     4261 ttttcttaca acgggcagcc gctttatcac tacatggggt gctctacatt cagtgaatac
     4321 accgtggtcg cggaagtgtc tctggccaaa attaatccag aagcaaacca tgaacacgtc
     4381 tgcctgctgg gctgtggcgt gaccaccggt attggcgcgg tgcacaacac agctaaagtc
     4441 cagccaggtg attctgttgc cgtgtttggt cttggcgcga ttggtctggc agtggttcag
     4501 ggcgcgcgtc aggcgaaagc gggacggatt atcgctatcg ataccaaccc gaagaaattc
     4561 gatctggcgc gtcgcttcgg tgctaccgac tgcattaacc cgaatgacta cgacaaaccg
     4621 attaaagatg tcctgctgga tatcaacaaa tggggtatcg accatacctt tgaatgcatc
     4681 ggtaacgtca acgtgatgcg tgcggcgctg gaaagtgcgc accgcggctg gggtcagtcg
     4741 gtgatcatcg gggtggcagg ttctggtcag gaaatctcca cccgtccatt ccagttggtc
     4801 actggtcgcg tatggaaagg ttccgcgttt ggcggcgtga aaggtcgttc ccagttaccg
     4861 ggtatggttg aagatgcgat gaaaggtgat atcgatctgg aaccgtttgt cacgcatacc
     4921 atgagccttg atgaaattaa tgacgccttc gacctgatgc atgaaggcaa atccattcga
     4981 accgtaattc gttactgatt tcccgcaggt ataccccgtc cacttcagac ggggttctta
     5041 atactctccc cgggcagccg tccgggggat taaccgtgag ataatgactg atggaactca
     5101 ttgaaaaaca tgccagcttt ggcggctggc aaaatgtgta tcggcattat tcccaatcac
     5161 tgaaatgtga aatgaatgtc ggcgtctatc tcccaccaaa agccgcgaat gaaaaattgc
     5221 cggtgctgta ctggctttca ggcctgacct gcaacgagca gaatttcatt actaaatcgg
     5281 ggatgcagcg ttacgcggct gagcacaaca ttattgttgt tgcgccggac accagtccgc
     5341 gaggcagtca tgtcgcagat gccgaccgtt acgatctcgg gcaaggtgcc gggttttacc
     5401 tgaacgcaac gcaagcgccg tggaatgaac attacaaaat gtatgactat atccgcaacg
     5461 agctgccgga tttagtgatg caacattttc cggcaacgac cagaaagtct atctctggtc
     5521 attctatggg cgggctgggc gcgctggtgc tggcgttacg taacccagat gaatatgtca
     5581 gcgtctcggc gttttcgccc attgtctccc catcgcaagt accgtgggga cagcaagcct
     5641 ttgccgcata tcttggtgaa aataaagatg cctggttgga ttacgatccg gtgagtctta
     5701 tttcacaagg tcaacgcgtt gcggaaatca tggttgatca ggggttgagt gatgattttt
     5761 acgcggaaca gctgcggact ccaaatcttg aaaagatctg ccaggagatg aatatcaaga
     5821 cgttaatccg ttatcacgag ggttacgacc acagctatta ttttgtctcc agttttattg
     5881 gcgagcatat tgcctaccac gccaataaac tgaatatgcg ttgataatag tgcacgactg
     5941 ccggatgcgg cgtgaacgcc ttatccggcc tacacttcgc ccgtaaaccg taggcctgat
     6001 aagacgcgca aagcgtcgca tcaggcatgg cgcacgactg ccggatgcgg cgtgaacgcc
     6061 ttatccggtc tacacttcgc ccataaaccg taggcctgat aagacgcgca tagcgtcgca
     6121 tcaggcatct gcgcacgact tatctatgtt taccacatca aatcatcagg cactttgaag
     6181 tcggcatacg gatcgtcttc atcctgctct tcggcactga gcgcgctgtg caacacaata
     6241 ctgctggcat cacgctgagc aattttatcg gcaacactgg cgggaataat ggtgtattca
     6301 ccttcgctat tgttatcgac caacaagcgg gcaatcgcca gacgaccatt aatcaactgc
     6361 gcctgtgtga gcttatcgac aaaaatcttc ttaatcagat tgccgtcagt gaagttaaaa
     6421 ccaatatcgc cattggcaat ggtgattcgg ttcatttcaa tgagctgctt cacctgggct
     6481 ttatattctt tcgccaacgc cgcttgtttt tgctgttcgc taagctgttt atcacgctca
     6541 agctgtgcct ttttattttc ttctaccgca gcccgagctt cacgcgcctg aacgcgcgat
     6601 tttttcgccg ttctctcgac cttcgccgct tttttgctgg tgactaatcc tgctttgagc
     6661 aactgctctt gtaaggtaag ttttgccatt tttgtttcca aacccgctga ataattttta
     6721 gcgagtatac ctgtaaacgc cgtgggtgta ttgggggcaa gtccctttcg cccctgctta
     6781 ataaggcgcg tcaggcatcg gcacacggct gcattcttga tctccgactc atcaaaataa
     6841 acaccgccaa cccagcaaca agaatacccg gtgcagaagc ggccattacg ccgaccgtgc
     6901 cagtgcctaa tgccagcatt ttcccggcca gtaacggacc gctcatagcc cccagacgcc
     6961 ctaccgccac ggctgttccc acacctgttg cgcggatctg cgaactgtaa aacaacggtg
     7021 ccagggcata caaaacgctt tgcccacctg tcgcaaacaa ccccgcgaca aatcccgcca
     7081 gcaacatacc gttaaatgac gacaccgttc caagcgccag cagcgaagct aacatgccgc
     7141 tataaatcag tagcgacatg gttactggac gcagcttatc catcaatgcg cccaacatta
     7201 acgtcccgct tgccgccccc atttgcaggg caaacatcac ccctgccgcc tgcgatggct
     7261 ggaatccttg ctccaccaaa agtagcggta gccagttgat caacatataa accaccagca
     7321 gagtgaagaa ataacacaac cacagcagca gcgtcgcggt tgccgtttct ggcgcaaata
     7381 aggcacgcag tggtggcgca gcctgttttt cgccagcgaa aaccgctgac tccggcagcc
     7441 agcgcattaa tagcggcacc agaatcaacg gcaccacacc acctacccaa aacaccgttt
     7501 gccatgctaa gtttgccccc gcgaaaccca gtgtcgccgc cagcgccgcg ccaatgggaa
     7561 caccgcaata catcaggctc actgccgtcc cacgaaaacg tggacccgcg gcttcagatg
     7621 tcagggcgat aagattcggc aacgccgccc ccagcccgac accggtcatc agccgcgcaa
     7681 agaccagtga ggggaaatcc caggcaatcg ccgttgccag tgagaacaaa ccaaacagcg
     7741 caactgagcc aatcaaaata cgcttacgac cataacggtc tgccagcatc ccgccaacca
     7801 acgcgccggg tagcaaaccg agtattccgg cgctaaatat ccagcccatt tgcattttat
     7861 cgagtgcgaa agcctgggcg atgccacccg ccgcaatgcc agccgcctga agatccagcc
     7921 cttccatcag agcgaccaaa aaacaaagcc cgatggtcag catcaggcgg gatgaagatg
     7981 atgaaggggt acgagtcgac ataagatgaa cctcattgcg agtgcaggtg aatcgcgact
     8041 gatacattgc cagccgcgta atcagaacta tttaatgact gcctacgggc gaaatgtagc
     8101 gaaatgtagg ccggataagg cgttcacgcc gcatccggca gtcgtgcgca gatgcctgat
     8161 gcgacgctaa cgcgtcttat cagacctaca aattctcacg ccatccgtag gccggacaag
     8221 gcgttcacgc cgcatccggc tgtcgtgctg ggatgggcat acaaaatgca cgttatcggc
     8281 agtcatcatt atttgttgtt gcgcagatcc agcgccacgt cgacgatcat atcctcctgg
     8341 ccgccaacca tccggcgttt gcccagctca acgagaatat ccaccgcact taagccataa
     8401 cgcgccgccg ccgtttcaca gtgacgcagg aagctcgagt aaacaccagc gtatcccagc
     8461 gccagcgttt cgcgatcgac tcgtaccgga cgatcctgca acggacgcac caggtcgtcg
     8521 gcggcatcca ttaacgcata gagatcggtc ccatgctgcc agcccagttt atccgcggcg
     8581 gcaataaaca cttccagcgg tgcgttacct gcgcccgcgc ccattcccgc gaggctggcg
     8641 tcgattcggt cgcagccctc ttccaccgcc gcgatagagt tcgccacgcc aagactcagg
     8701 ttatggtgag cgtgcatgcc ggtttgcgtt tctggtttca acactgcttt cagggcgcgg
     8761 aaacggtcac ggatatcgct catgttcatc gcaccgccag aatccaccac ataaatacag
     8821 gtcgcaccgt agccttccat cagctttgcc tgcttggcga gattctccgg cgtggtcata
     8881 tggctcatca tcagaaaacc aacggtgtcc attccgagct cgcgggcata ctgaatatgc
     8941 tgggcggaaa catcagcttc ggtacagtgc gttgccacac gaaccacccg cgcgccagcc
     9001 tgccaggcat ttttcagatc gtgaatagtg ccgattcctg gcagcaacaa cgtcgcgatt
     9061 ttggcgtgcc tcaccacatc cgccgccgct tcaatccatt caaggtcgct atgtgcgccg
     9121 aaaccatagt taaagctgga accttgcaaa ccgtcgccgt gggccacttc aatcgaatcc
     9181 acgcgggcat cgtccagtgc tttggcaatc tggcgaacgt tttccagcga atactgatga
     9241 cgaatggcgt gcataccgtc acgcaatgtg acgtccgaga tataaagttt tttaccgttc
     9301 atgccgcttc tcctgccttg cgcgccagtg actgggccat tttttccgct gtcgccagcg
     9361 cactggaagt cataatatcg aggttgcccg cataggcagg cagataatgc gctgcgcctt
     9421 cgacttccag ccagaccgct gttttcagtc cggagaattg ccccacgccc ggtaaattga
     9481 ccggtttatc ctgcgggata acttcaaact gcacgcgctg tttcaggcga taacccggta
     9541 cgtaagcctg caccgcctca gccatttcat tgattgaggc ttcgatatca tcttgtgaag
     9601 cttcgtcgct caatacatac accgtgtcac gcatcatcaa cggtggctct gctgggttaa
     9661 gcacaataat cgccttccct tttgccgcgc cgcccaccac ttcaatggct cgggaagtgg
     9721 tttccgtaaa ttcatcgata ttggcacgcg tgccaggtcc ggcagattta ctggcgatag
     9781 aagcgataat ttcggcgtaa tgaacacgcg ccacgcgtga aactgccgcc accattggaa
     9841 tggtggcctg gccgccgcag gtgaccatgt tgacgttcag ttgatcgacg ttcgcctcga
     9901 ggttaaccac cggcacgcag taagggccga tggcagcagg cgtcaggtca attaagcgaa
     9961 tatccggttt cgcttcgcgt aaagcggcat cgtttttcac atgcgcgccc gcgctggtcg
    10021 catcaaatac aatgtcgata tcagcaaatt caggcatgtt catcagcccg atcacccctt
    10081 catgggtggt ggcgacgccc atacgtctgg cacgcgccag accgtcggac tgaggatcaa
    10141 tgccgaccat caccgccatc tccagatgct gaccgtgacg caaaatttta atcatcagat
    10201 cggtgccaat gttgccagaa ccgataatgg cgactttacg cttactcatg acagacttcc
    10261 ttttggggct gcgcttgaaa atgtcgcagc aactgaacct atgccttcaa tatgggcttc
    10321 aaaacgatcg cccgcattca ccgccaccat cgggcctaat gccccggtaa gaatgatatc
    10381 tccggcgcgc agcggttcac ccagactggc cattttgcgt gccagccaga cggcggcatt
    10441 aagcggatgt cccaggcatt cgctgccgcg cccgctggaa acctcttcgt tattacgcgt
    10501 catcttcatg gcgcagtttt tcaggtctaa ccccgccgga cgttgcgccg gaccgccgat
    10561 gacatacacc ccgcaggaag cgttatctgc cacggtatcg acaaactgaa tcgaccagtc
    10621 gcgaatgcgg ctccccacca cttccagcgc cggaagtacc cattcaatgg cgttatacag
    10681 ttcgtcgaag gtgatatcag ttgcgggtaa atcgcggttc aacaccagtg cgatctccgc
    10741 ttcaatgcgg ggttgcagaa cacgggaaaa aggaatgatt tcgttatcgc cataacacat
    10801 gtcggcaaat aacgtcccaa aatccggttg atcaacgccc agttgttgtt gcactttcgg
    10861 atgcgtcagg cccactttac gccctaccac gcgacgcccc tgcacaacgt catattgcac
    10921 atttatgtgc tgaatggcgt aggccgcttc agcgtgatcg ataccaatca gatcgcgcag
    10981 cggcgcaatc gcttcgccct gctctgcggc gcggcgtaaa tccgccgcca gttgctcaag
    11041 agtatgcttc gtcatgacca ttccttaagg gcgtgcgagg aaattcagca ccaattgatt
    11101 gaaagcgtcg gcatgttccc actgcgccca gtgaccacag tcgcggaaga tatgcagttc
    11161 agaaccggca atgccggaca gcagacgcag acccgcatcc atcggcacaa agcggtcgtt
    11221 gcgcccccag acaatcaggg tttgcgcttt gatttccgcc agacgtgggc caaaatccgg
    11281 gaactgtttc ggattagctt ccaggctctt aacgaagttt tccaggtgat cgcggcgcga
    11341 cagcatatta ttcaggcgcg cttcaaacag ggcgtcggtc aaatcgctgg tatcgaaaac
    11401 gaagatatcc atcatcagct tcaggttttc gatagtcggc tgacgataaa gctgattcag
    11461 tcgcttaata ccttcggttg gcatcggcgt aaacaaactc atgccgcccg taccgccgcc
    11521 catcagcacc agtttgccga cgcgctccgg ccagttaagg gtgaacgcca cagaactatg
    11581 gccgcccatc gagttgccca gcaggtggat tttggcgata tccagttgat ccaccacgct
    11641 tttcaggatt cgtgcattaa gatccgatcg tgaaccacta ttaacgatcg aatcgctttt
    11701 gccccaaccc ggacaatcca gcaggatcac ccgatagccc gcctctacca gcggatcgat
    11761 attgcggctg aagttcgccc agccagtagc acccgggccg gaaccatgca gcaggacaac
    11821 ggtttcgtcg ccttgtccgc agtcattaaa atggatgcgc agcgttttac ccgcttcttc
    11881 tacattcaga aaacggctgg tggcggcttc ggtttgtggc tgataactca tcatcttctc
    11941 ctgcatattc agttctctgt tctggcgctt aacgagccaa atccggcaat ccactccggg
    12001 attgggcggt aataacgccc ttcgctacgc cagttgccaa atgcagaaat agcggcaaaa
    12061 gcggcgaccc aggttttgat ttcatgtgtc gactttccgg caatggcgga aagctcttcg
    12121 ttactgacgg catccagttc ctgtatgcgt ccctgctcca gcaaagtcat gaactggtta
    12181 tcccaaatcg ggttgagcgg atgcagagtt ctctgatcct caacaaactt ctcagcggcg
    12241 ctaatcaccc tttgctgacg caattcgcgc tcactggcgg gtaaatcttt cccgctcccc
    12301 aacagacggt cgcgcatatg ggcatcggct ttcgccagtt cgggcaccgg cggctgatgg
    12361 gaaagcccac cggaacccag gaacagcacg cgtttattga gagtgctggt gaaacgtcca
    12421 atggcttcac ccaacatgcg ggtacgctgg aaaccgggca gcggcgtggc gacaccgttg
    12481 atgaacacag gcagaactgg caccttatcc agcccaccga gcaggaactc cagcggctgg
    12541 gcgaacccgt ggtccacctg catacagtaa gaaacggcaa gatcgatccc gctcttcatg
    12601 acagcatgcg cacaggcctc cgccagctcc acaggcacgg gcagctctcc tgccgcactg
    12661 ccgaaatcac caattgccgt cgctccaacg cctaaacaga acggtggcat cacgtcatag
    12721 aaaaagccgt tgtagtgatc tggcgcaaac agcaccacca gttcagggga gaaagccgca
    12781 atacgctcgc gggcgctggc aatcacgcca ttgacctcat cgagtacctc ttgcgccggg
    12841 tcgacatacc ccaccaacgg cgagtgggaa agacagtgaa gataagcgtg catatcaggc
    12901 taccttttcg acagaaacgt cggcatcagg gcgggtcagc gtcatcaccg acgccagttt
    12961 attcagcgta ttgcccaggg tttgcggaat ggcggtggcg gcaacaaagc gatccgggcg
    13021 catcaccacc agcgaagcat tgtgttgcgc gaaccagcta cgcagacgac cttgcgtatc
    13081 gcccacgtgt agcacgccgt cgtggttatc ctgtgcggta tgaatttgca cttccggcac
    13141 cacctgaatg aagcgtgtgc ccaacgcgcg ccactgctgg atttgctcgt cgctcatccc
    13201 ccacagtgga ttgcatcccc agccaattac cgcgaagttc gcgccgatcg cgttatcgag
    13261 cagcgtcacg tcgccgtttt ccagcgtgac tttcggctga ataaacatct tgccgaccgg
    13321 agagtgcttc gcttcgccct cacgcaccag cgcaccgccg taatattgcg gcatcggctt
    13381 gaagcgcatt tcgaggaagt agcgttttac tggcggcaga taattcaaca gccaggaaac
    13441 gccgtcacgt aacgtaccct gccagcgttt cggcggagcc agcacgttgc ccgccgtcac
    13501 ggacaggtca atcatcgctt tggcgtgatc gcgacgttct tgttgatagg tatcgagcag
    13561 cgcatcgcgg gctttcccct ggataaccaa cgccagtttc caggcgaggt taaaggcgtc
    13621 gcgcatacca ctgttatagc cctgcccctg ccacaccggc atgatgtgcg cggcatcgcc
    13681 cgccagcagt acgcgatcaa tacggaaacg ttgcgccagt cgcgcgttat gggtgtagac
    13741 acgctggcga atcaattcaa cattgtccgg attaggcagc actttgctta acagcttgcg
    13801 catattttgc ggctcacgca gctgctcttc ggtttctccc ggcatcacca taaattcaaa
    13861 gcgacgtacc gcatgaggca gcgcggcaga aacatacggg cgcaccggat cgcaacacaa
    13921 atagatatgc ggcgtactta acggatcgtt ggcgatatct accacaatcc actgatttgg
    13981 cgcagtttta ccttcaaacg gcacattcag ggtgcgacga acaaaacttg ccccaccatc
    14041 acaggccacc agccactggg ctttgatcgt ttcccgctgc ccttctgccg ttttcaggtg
    14101 caaggtcact tcgtcatctt gctggctgaa ggcctccagc tcgcgggaaa acaagcagcg
    14161 cacattcgga aaacgcgaca acccttccag catcaccgca tcgacctgtg gctgaataaa
    14221 ggcgttacgg cgcggccagc caaattcatc ggtcattggc tgaatatcag caaaacagcg
    14281 gccttttggg gtgagaaaac gcatcgcgtg ccacggtgta gtgtgcggca gaacattctc
    14341 gaccaggccg accgactgca tggtgcgcag cgcctcgtca tcaataccaa tcgcacgcgg
    14401 gtagtcgatc aacttatcga gtttctccac caccagcacg tcaatgccca tctgaccgag
    14461 atagttcgcc atcatcagcc caaccggacc ggcaccagcg atcgccacct gaacgctatg
    14521 gttaacagca ggctggatgt cagggtgttg tattgccatt tctgcacaac gttgttttaa
    14581 ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa accgtgcgcg
    14641 gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc ggtgccagcg
    14701 tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga ctgctggaga
    14761 cgctgcagga agagggatat gtccgccgta gcccctccga tgacagtttt cgtctgacca
    14821 tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct gcactggcgg
    14881 ccccgctgct gggcgatctg ttgcgcgaag tggtatgacc aacagatgtg tccaccctgg
    14941 atgttgatgc aatggtggta cgcgaaacca cccaccgttt cagccgctta tcctttcacc
    15001 gggcaatggt cgggcgacgt ttaccgctcc tgaaaaccgc ctcgggcctg acctggctgg
    15061 ccttttgccc ggaacaagaa cgcaaggaat taatcgaaat gttagccgcc cgccccggtg
    15121 atgactatca actggcacga gaaccgttaa agctgcaagc cattctggcg cgcgcgcgca
    15181 aagagggtta cggacagaac taccgcggct gggatcagga ggagaagatc gcctctatcg
    15241 ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg tatatggcga
    15301 gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa cgggtagcaa
    15361 aacagatcga agaaggggtt gaatcgcagg ctattctggt ggccggacgg cgaagcggcg
    15421 tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gtggtatggc atgatagcgc
    15481 ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata cgatgtcgca
    15541 gagtatgccg gtgtctctta tcagaccgtt tctcgcgtgg tgaaccaggc cagccacgtt
    15601 tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac
    15661 cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt
    15721 ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg
    15781 ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg
    15841 gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac
    15901 caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc
    15961 tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc
    16021 gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt
    16081 tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt
    16141 cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg
    16201 caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg
    16261 ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta
    16321 gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa
    16381 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
    16441 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg
    16501 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
    16561 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
    16621 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtgaaat
    16681 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg
    16741 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    16801 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    16861 cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag
    16921 cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct
    16981 caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta
    17041 cggtcaatcc gccttttgtt cccgcggaga atccgacggg ttgttactcg ctcacattta
    17101 atattgatga aagctggcta caggaaggcc agacgcgaat tatttttgat ggtgttaact
    17161 cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac agccgtttgc
    17221 cgtctgaatt tgacctgagc gcatttttac gcgccggaaa aaaccgcctc gcggtgatgg
    17281 tgctgcgctg gagtgacggt agttatctgg aagatcagga tatgtggcga atgagcggca
    17341 ttttccgtga cgtctcgttg ctgcacaaac cgagcacaca aatcagcgat ttccatgttg
    17401 ccactcactt taatgatgat ttcagccgcg cggtactgga ggcagaagtt cagatgtacg
    17461 gcgagctgcg cgatgagctg cgggtgacgg tttctttgtg gcagggtgaa acgcaggtcg
    17521 ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc
    17581 gcgtcacgct acgtctgaac gtcgaaaacc cggcgctgtg gagcgccgaa atcccgaatc
    17641 tctatcgtgc ggtggttgaa ctgcacaccg acgacggcac gctgattgaa gcagaagcct
    17701 gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc
    17761 cgttgctgat tcgcggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg
    17821 atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc
    17881 gttgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc ttcggcttgt
    17941 acgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg
    18001 atgatccgcg ctggttaccc gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc
    18061 gtaatcaccc gagtgtgatc atctggtcgc tggggaatga gtcaggccac ggcgctaatc
    18121 acgacgcact ctatcgctgg atcaaatctg tcgatccatc ccgcccggtg cagtatgaag
    18181 gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg
    18241 aagaccagcc cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg
    18301 gagagacgcg cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg
    18361 gcttcgctaa atactggcag gcgtttcgtc agtacccccg tttacagggc ggcttcgtct
    18421 gggactgggt ggatcagtcg ctgattaaat atgatgaaaa tggcaatccg tggtcggctt
    18481 acggcggtga ttttggcgat acaccgaacg atcgccagtt ctgcatgaac ggtctggtct
    18541 ttgccgaccg cacgccgcat ccagcgctga cagaagcaaa acaccagcag cagtttttcc
    18601 agttccgttt atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg
    18661 ataacgagct tctgcactgg tcggtggcgt tggatggcaa gccgctggca agcggtgaaa
    18721 tgcctctgga tgttgctcca caagataaac agttgattga actgcctgaa ctaccgcagc
    18781 cggagagcgc cggacaactc tggcttacgg tacacgtagt gcaaccgaac gcgaccgcat
    18841 ggtcagaagc cggacacatc agcgcctggc agcagtggcg tctggcggaa aacctcagcg
    18901 tgacactccc cgccgcgccc cacgccatcc cgcaactgac caccagcgaa acggattttt
    18961 gcatcgagct gggtaataaa cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga
    19021 tgtggattgg cgctgaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac
    19081 cgctggataa cgacattggc gtaagtgaag cgacccgcat tgacccgaac gcctgggtcg
    19141 aacgctggaa ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag
    19201 atacacttgc cgacgcggtg ctgattacga ccgctcacgc gtggcagcat cagggaaaaa
    19261 ccttatttat cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg
    19321 ttgatgttga agtagcgagc gatacaccgc atccggctcg gattggcctg acctgccagc
    19381 tggcgcaggt agcagaacgg gtaaactggc tcggattagg gccgcaagaa aactatcccg
    19441 accgccttac tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc
    19501 cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc
    19561 cacaccagtg gcgcggcgac ttccagttca atatcagtcg ctacagccaa caacaactga
    19621 tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg
    19681 gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtttca gcggaattcc
    19741 agcttagcgc cggtagctac cattaccagt tggtctggtg tcaaaaataa taataaccgg
    19801 gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca
    19861 caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg ggggcctact
    19921 tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta
    19981 ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt
    20041 ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt
    20101 ttgcgc
//
LOCUS       DAZPBM010000070        19924 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPBM010000070 DAZPBM010000000
VERSION     DAZPBM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19924
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.070"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHUZQ_002264"
     CDS             complement(<1..330)
                     /locus_tag="ACHUZQ_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR1025951.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUZQ_002265"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUZQ_002265"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1025952.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUZQ_002266"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUZQ_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1025953.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUZQ_002267"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUZQ_002267"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1025954.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUZQ_002268"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUZQ_002268"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1025955.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUZQ_002269"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUZQ_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1025956.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUZQ_002270"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUZQ_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1025957.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUZQ_002271"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUZQ_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1025958.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUZQ_002272"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUZQ_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1025959.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUZQ_002273"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUZQ_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1025960.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUZQ_002274"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUZQ_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1025961.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUZQ_002275"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUZQ_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1025962.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUZQ_002276"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUZQ_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1025963.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13115..13543
                     /gene="uspC"
                     /locus_tag="ACHUZQ_002277"
     CDS             13115..13543
                     /gene="uspC"
                     /locus_tag="ACHUZQ_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR1025964.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13550..14974)
                     /gene="otsA"
                     /locus_tag="ACHUZQ_002278"
     CDS             complement(13550..14974)
                     /gene="otsA"
                     /locus_tag="ACHUZQ_002278"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR1025965.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14949..15749)
                     /gene="otsB"
                     /locus_tag="ACHUZQ_002279"
     CDS             complement(14949..15749)
                     /gene="otsB"
                     /locus_tag="ACHUZQ_002279"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1025966.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(15916..16902)
                     /gene="araH"
                     /locus_tag="ACHUZQ_002280"
     CDS             complement(15916..16902)
                     /gene="araH"
                     /locus_tag="ACHUZQ_002280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1025967.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITILATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(16917..18431)
                     /gene="araG"
                     /locus_tag="ACHUZQ_002281"
     CDS             complement(16917..18431)
                     /gene="araG"
                     /locus_tag="ACHUZQ_002281"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1025968.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(18501..19490)
                     /gene="araF"
                     /locus_tag="ACHUZQ_002282"
     CDS             complement(18501..19490)
                     /gene="araF"
                     /locus_tag="ACHUZQ_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR1025969.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT         4805 a         5373 c         4747 g         4999 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagggcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
    12601 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
    12661 tgggtgtatt tcaatatgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
    12721 gaataatcga aatgggatgt tgcgcacagt taaaataaat caccgtaaat aatcatctgc
    12781 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
    12841 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat gaaatgtttt
    12901 acatatatta accacttgtt taatgcggag aatcagtaca ctattttctt ataattacat
    12961 ttgaaattat atgatcaccg ggagtgattg attaattgtc tttactaata atcagactaa
    13021 tatttacctg tttgaccgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
    13081 tgtcacctga agttcacaga ataaggaaca ggttatgagc tatagcaata ttcttgtcgc
    13141 tgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta tcgccaggcc
    13201 agtaaaggga cacatcagtt taattactct cgcttccgac ccggaaatgt acaatcaatt
    13261 agccgcgccg atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc aaagctttct
    13321 tgataagtta attcaggatg cggggtatcc cgttgacaag acgtttattg cctacggtga
    13381 gttaagcgaa catattctgg aagtatgtca caaacatcat ttcgatttgg tgatttgcgg
    13441 taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag tgattgcctc
    13501 aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgctcc tacgcaagct
    13561 ttggaaaggt agcaacttta tcgcgctgct ggctttccgc gcttcgcgga actatctgct
    13621 ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg ataacgtcca
    13681 gcatttctgc atgacgggaa atacgttccg ccagcgacat agtcaatgca cgatccagcg
    13741 cagctgcaac ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt aactcgtttg
    13801 ccgctcccgc aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc tgagcagcaa
    13861 catactcttt tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag cccacgtcag
    13921 agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa taataaagcg
    13981 gcgtccagcc taattgcccg tatttaccat taattcgtcc agcttcattt tcgagctgat
    14041 gacgaatatc ctgataggct tgcacatcac cacgcgacgt tggtgcaatc tgggtataac
    14101 gaattttacc atgatgctgc ggatattttt ccagcaacgc ttcataggcg agaaaacgct
    14161 ctggcaaacc tttggaataa tccagccgtt cgacagaaaa gatattttgt acgtttttca
    14221 gttccgcttt aagttgcgcc agttttggcg gcagtggccc ggcagcctgt ttggctattt
    14281 ctttcggttc aatgccgatc gggtagactt ctgttcgaaa tgctttgccc caggctgtat
    14341 ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aagacaatcc aggaacgcca
    14401 gacgatcgtt ttctgtctgg aaacccagca aatcataatc acaaagctgt tcaagcaagg
    14461 tgtcatatgt cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata tgcagaaaga
    14521 aaccaatgcg attattcact ccccgtttgc gtaattcatg cgcaaatggc aacaggtgat
    14581 aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta tctgccagca
    14641 acgcatttac gcgtagatag ccgtcccagg caggacgctg aaattgcacc agatcgagcc
    14701 gataatgaaa agcgggccag agaacggcat tggagaattg gttgtagtat tcgtcaaggt
    14761 cctgttcgct gaggttaaaa gaggcccacg taatgttacc ttttttcacc ttttttagcg
    14821 gctgatcctc attccctgtt tcaccactcc agccaaacca cagtccgcct gcggctttca
    14881 gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg tctggtggtg
    14941 caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt attttctctt
    15001 ttttgttgta atgcggtggt tatcatttca agccagctcc agacatccgg cacacccgcc
    15061 agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc gcccagtcgg
    15121 ttaacgactg cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc
    15181 ccgataaagg gagcttcctg cataaaagct gcaattgcct cacctttact ggtacctctc
    15241 ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca gatctgagta
    15301 atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg ataatgcagc
    15361 gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgagcgat gactgtatgc
    15421 agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg tgttttacca
    15481 ttgatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata aggttttgcc
    15541 agtgcgtcaa gctccaccat tgagcgccct gatatcaatg ccaatgcacc atcacttgcg
    15601 gttgccagta gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg atcgggatgc
    15661 ggtttgattt ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt cgcggatagt
    15721 tcaggggttt cggttaacgg ttctgtcacc cggctctcct ccttctcttt tcttattgtt
    15781 ttgcgcagaa cgcgctttac agacatgtaa gtatagacag tgtgacgggg gtcgccattt
    15841 gcaatgaaga acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa cgttaaattg
    15901 ttgtggaaaa aagcatcaga cagtgcgttt cgctttttgc ttgtaacggt cgaagatcac
    15961 cgctgccagc aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag
    16021 gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat atgagatttt
    16081 tccgatgcca cctttcagag aaacgccacc taaaacgcag gcggagataa caataagctc
    16141 ataaccaatc gacgtcattg gctgcccact agtcatacgt gaagccagaa taattccggc
    16201 tatcgcagat accaggccag agagaacaaa gataataatt ttggtgcgaa caaccggtac
    16261 acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtgttac gaccaaaggt
    16321 ggttttattc agcaacaaac caaagataat cagacacgcg acggtgagcc agattggcgc
    16381 aggcagaccg aaccagttag cgtaaccaag ggcaaagaag ctttcatctt cgataccgac
    16441 cgctttaccg tctgaaatga tgtacgccag acctcgaaca atctgcatcg ttgccagtat
    16501 tgtgatcaga gcatttattt tcagtttggc gataacaaag ccattgacca ggccacagag
    16561 aatgccaagc agcaaccccg ctgccacgcc aatccacagg ctttcagtca ggttgataac
    16621 caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt caaagtcacc
    16681 ggaagcaagg cagaacaaca tgccacacgc caccatcccc gacatggaaa ttgccaggcc
    16741 caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac aggcgataaa
    16801 gagcaccgca aacaccacca gcatgccgta ctgatcccaa atacgcccga agctgaatga
    16861 cgacttaggt gcgccagaac ccgatgtaga aacagaagac atcatactct ccttactcag
    16921 gcaactgcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc atctgcctgc
    16981 tcgtgtaaca attcaccggc gatttcacct tcccgcatca ccacaatccg gtcggcaacg
    17041 ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacaccctg cgccgccagc
    17101 gcataaatta cgttatatat ttcgtgctta gcaccaacat caatgctgcg cgtaggttca
    17161 tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc tttttgctga
    17221 tttccgcctg agagattcat gatcagttgc tcagcgcccg gcgttttgat gttgagcgaa
    17281 cgaatgtgct gatcggcatt gttttcttcc caaccgttgt tgattacaca accgccgagc
    17341 acatgtttgc gtctggcact gatgttgata ttgtcgcgaa cggagtgcac gggaataatg
    17401 ccttccgctt tgcgatcttc cgggcagagc atcatgcctg cggcaatggc gtggctcggt
    17461 ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat ttgcgtcccg
    17521 ccaaacaagc ctttcattaa ttcgctacgc cccgccccta ccagaccaaa cagcccaaca
    17581 atttcaccac tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc tttcacagca
    17641 tcaagacgca ggcgctcctc gccataacta cgaggttgcc agccgtagat atcgccaatg
    17701 tcgcgcccga ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat atcggtaaag
    17761 gttttgacat aacgtccatc tttaaagaca gtgatggcat cgctgagggc aaatatttct
    17821 tccatacggt gagaaacgta taagatcacc cgcccctctt ttcgcagttc acgaataacg
    17881 cggaaaagat tgtcgatttc gcgggcagaa agggagctgg ttggctcatc aaaggcgata
    17941 attttagcgt tacgcgccag cgccttggcg atttcaacca tctgccactg accaatggag
    18001 agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaag ttgtaaaccc
    18061 gcctcataat tcagcaatga gcgattcaca atgccgcctt tatgcggcag ctggccgaga
    18121 tagatgtttt ccgcgacggt catttccggc acgagatgca gttcctggta aataatcgcc
    18181 acacccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc attaatcact
    18241 acagaacccg tggttggcgc atagttgccg ctgaggattt ttaagagagt tgattttcct
    18301 gcgccatttt cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa actaatatcc
    18361 gtcagcgcct taacgccggg aaacgtttta ccgatgccgc gaaatgagag atacggggta
    18421 gactgttgca taacgtctcc gtgaatcact ggtcgttcac accccctctg catcatgcag
    18481 aggggaattt ttccggcaaa ttacttaccg cctaaacctt ttttctccag ttcttcttta
    18541 aagttgtcac gcgtgatcag caccacgtcg gtaacttcgg taaattttgg cggttcaacg
    18601 tcttttgcta cccagttgta aagcatttcg ctggatttat agccatgtac gtccgggctt
    18661 ggcagcaggg aaccgtagaa gccggttgcc tgtgctttag acagttcgct caacgcatcc
    18721 acaccgttaa tgccaatgcc gatgatatcg gcagctttaa agccctgacc ttccgtcgcg
    18781 cgtacgccgc ccagcacggt gctgtcgttc ataccgacga tcagccaatg tttaacttcc
    18841 ggatgttgaa ccagcattga gttggcagcg tcaaatgccc ccgggatgtc gttagatttg
    18901 gtaggtacct gataaatttg tttttccggg aatccggccg ctttcagcgc atccatagaa
    18961 cccgttgtac ggcgacgtgc cgtatccagt tcgttggcgg taatcgccat caccgcgctt
    19021 tctttgacat cccagccacg tttctgcatc tctttataca gttcctgacc ctgacgttcg
    19081 ccaattttag tcgccgccat catcaccagc ggaacggtat ccattggctt acctttggcg
    19141 ttaacaaact ggtcatccac ggcaatgact ttcatatcgt agccacgcgc tttcgcgacg
    19201 atggcagagc cgagtttggg gtccggagta caaataacga aaccttttgc gccactggca
    19261 gccaggctgt cgatcgcgtt caatgttttt tcgccatccg gcacggcaat cttaataacc
    19321 tcaaacccta aatccttccc ggctttatcg gcaaacttcc attcggtctg gaaccacggc
    19381 tcttccggtt gcttcaccag aaaaccgagc ttcaggttct ccgccatagc ggattgtgac
    19441 ataacggctg ccagaccaat ggctgccagg gctttagtaa atttgtgcat ggttctctcc
    19501 agctttagtg tcgttttgtg tagggcaaaa acgaatgact ttcgttaaat taatcggaaa
    19561 acaaagcatt accttttatc taaaagataa gtgaccgtgt tgacatagtt ttagcgagaa
    19621 attaattctc cataggagag caatatcaca tcgcagaatt acagtgagaa cgtgcataaa
    19681 tttagcggga aaagacataa gggaaagcca atttgtcaga caaattgtcg aatgcacagc
    19741 agattaatcc ataagattag cctggaaatc cttgttgtct ttggtaccca tgcgggatgt
    19801 cttcttttta accagtcaat aggccgcatt acctggcgtt gagtttttga aatggtgtaa
    19861 taaccgcaac tcaaagatgt ggaaaatgca cgtcattcat ttcgccatta attatcactg
    19921 tgct
//
LOCUS       DAZPBM010000071        19764 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPBM010000071 DAZPBM010000000
VERSION     DAZPBM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.071"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..369
                     /locus_tag="ACHUZQ_002283"
     CDS             <1..369
                     /locus_tag="ACHUZQ_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1025970.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
     gene            complement(403..813)
                     /locus_tag="ACHUZQ_002284"
     CDS             complement(403..813)
                     /locus_tag="ACHUZQ_002284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1025971.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            960..1811
                     /gene="hchA"
                     /locus_tag="ACHUZQ_002285"
     CDS             960..1811
                     /gene="hchA"
                     /locus_tag="ACHUZQ_002285"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1025972.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHUZQ_002286"
     CDS             complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHUZQ_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1025973.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHUZQ_002287"
     CDS             complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHUZQ_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1025974.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHUZQ_002288"
     CDS             4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHUZQ_002288"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1025975.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4603..5607
                     /gene="msrP"
                     /locus_tag="ACHUZQ_002289"
     CDS             4603..5607
                     /gene="msrP"
                     /locus_tag="ACHUZQ_002289"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR1025976.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHUZQ_002290"
     CDS             5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHUZQ_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1025977.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            6500..7150
                     /gene="zinT"
                     /locus_tag="ACHUZQ_002291"
     CDS             6500..7150
                     /gene="zinT"
                     /locus_tag="ACHUZQ_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1025978.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7493..8023
                     /locus_tag="ACHUZQ_002292"
                     /pseudo
     CDS             7493..8023
                     /locus_tag="ACHUZQ_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(8593..8682)
                     /locus_tag="ACHUZQ_002293"
     tRNA            complement(8593..8682)
                     /locus_tag="ACHUZQ_002293"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:Ser,seq:cga)
     gene            8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHUZQ_002294"
     CDS             8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHUZQ_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1025979.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9674..9749
                     /locus_tag="ACHUZQ_002295"
     tRNA            9674..9749
                     /locus_tag="ACHUZQ_002295"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9707..9709,aa:Asn,seq:gtt)
     gene            10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHUZQ_002296"
                     /pseudo
     CDS             10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHUZQ_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(17390..18442)
                     /locus_tag="ACHUZQ_002297"
     CDS             complement(17390..18442)
                     /locus_tag="ACHUZQ_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR1025980.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            18757..>19764
                     /gene="shiA"
                     /locus_tag="ACHUZQ_002298"
     CDS             18757..>19764
                     /gene="shiA"
                     /locus_tag="ACHUZQ_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR1025981.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         5513 a         4657 c         4411 g         5183 t
ORIGIN      
        1 gcaatgatgt atgctgaaac ccgcaatatg acaccatatg gtaatgtggg tatcgcaaat
       61 aaaacccaga actttgaagc tgtagcacag tatcagttcg attttggtct gcgcccatct
      121 cttgcatatg tttattcaaa aggcaaagac ctcggcggta acgactataa caacaatggt
      181 catcaagagt atgttgatca ggatctggtc aactatgttg aaattggagc gacctactat
      241 ttcaataaga acttctcaac atatgttgac tataaaataa acttgttgga taaagatgac
      301 gatttctatg acaacaatgg catcgcaaca gatgacgttg ttggtgtggg cttagtctac
      361 cagttctaat cagccaacgt gctgcggcgt gttactgtaa aactaaacac tagcctgagt
      421 tcgtccctca tcgaattcag gcttttttat tggcatttca tctccatctg cggcacacgc
      481 aattgccaat gcatatcgat acagcacctt accctgcccc tcctggcaca ataacatcaa
      541 ccagcgactc ttcaaaccaa cacccaaatg catttcttta tgaattaatc cgtaacaaac
      601 acgcttccca tatagaaata tttgcaaaaa tagtgcatga aataatcttt tctctaccaa
      661 tccgcgatat aaatattcat cagtaccaca atatttcaca gcactaaatc tctccccgcc
      721 gccccgtacc tttgataatg gtctaaaatc attgaggcca cttgcgacga cgttgctcaa
      781 taaggaaaat ttggcgtgaa taatattcaa caccagtgca gcatcatcat tacatactta
      841 cacccgccga ctaccgcttt cgatatattg actcaatttt ccagactggt taaaaatagc
      901 gaccagctca gtcgcaaata tagtgactac cctaactaag caacaataag gaatacacta
      961 tgactgttca aacaagtaaa aatccgcagg tcgatattgc tgaagataat gcattcttcc
     1021 cttcagaata ttcgcttagc caatatacca gtcccgtctc tgatcttgat ggcgtggact
     1081 atccaaaacc gtatcgcggt aagcataaaa ttctggtgat cgccgcggac gaacgttatt
     1141 tgccaaccga taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc
     1201 cgttgtatca tctccatgct gctggtttcg aattcgaagt ggcgaccatt tccggtctga
     1261 tgaccaagtt tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg
     1321 agcagcataa atcgttgttc cgcaatccga agaaactcgc ggatgttgtc gccagcctca
     1381 acgctgatag cgaatatgca gcaatctttg ttcctggtgg tcatggcgca cttattggtt
     1441 taccagaaag ccaggacgta gctgctgctt tgcagtgggc aatcaaaaat gaccgttttg
     1501 ttatctccct ttgccacggc ccggcggctt ttctggcgct tcgccacggc gataacccac
     1561 tgaatggtta ttccatttgc gcattcccag acgccgcaga caaacaaacg ccagagatag
     1621 gctatatgcc gggtcatctc acctggtact tcggcgaaga gctgaagaaa atgggcatga
     1681 acattattaa tgacgacatc accgggcgag tacataagga ccgtaaagtt ctcaccggcg
     1741 acagtccttt cgcagcgaat gcgttgggta aactggcggc gcaggaaatg ctggcagctt
     1801 atgcggatta atccctgtta cgtttgaatg taaacgcata atcaatcgca gccacgttaa
     1861 tactcacgac gtggctgcgt tttttcatgg ttgatttatg gcgtactgaa gccctatgtt
     1921 aatttctttg cggtaacata atccggaaca cattatgctt attgagatag tgatacgtag
     1981 cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt ccctgaccta
     2041 cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca ggctcatgaa
     2101 ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca agaaaactgg
     2161 ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata agattcgata
     2221 acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg accttaaagc
     2281 aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg acttccttat
     2341 tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg gcaagaaaca
     2401 gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg gcaattgttt
     2461 tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga cccagtaatg
     2521 cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt agacgctcaa
     2581 aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt tttagttctc
     2641 tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc gcttccgtta
     2701 caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga cttaatactg
     2761 agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac tgttcaagca
     2821 tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc gaaacttcat
     2881 caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg taaatgccag
     2941 ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg acatttgtcc
     3001 ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga ctaacatcca
     3061 tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt aacaactgct
     3121 tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac tcacttgcca
     3181 ggccattata gagagtccag acaattccag cgccagcaac agacagcagc aatataaaaa
     3241 gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg ctacgaatga
     3301 atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat caacttttgc
     3361 gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt tgattcccca
     3421 aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg ccagtagcca
     3481 aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc tcacactttg
     3541 acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag cgtgatgttg
     3601 ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata
     3661 atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc ttgcagtaag
     3721 gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc agccatccat
     3781 acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg caagataaag
     3841 cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa gcccctgcgt
     3901 tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga aaataatatg
     3961 ccataaatca tatgttatag aaacagccta gttcattaca aaattgtaat gctgctgtaa
     4021 ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa ggatataaac
     4081 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
     4141 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
     4201 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
     4261 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
     4321 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
     4381 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
     4441 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
     4501 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
     4561 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
     4621 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
     4681 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
     4741 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
     4801 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
     4861 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
     4921 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
     4981 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
     5041 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     5101 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     5161 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     5221 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     5281 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     5341 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     5401 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     5461 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     5521 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     5581 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     5641 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     5701 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     5761 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     5821 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     5881 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     5941 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     6001 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     6061 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     6121 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     6181 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     6241 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     6301 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     6361 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     6421 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     6481 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     6541 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     6601 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     6661 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     6721 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     6781 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     6841 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     6901 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     6961 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     7021 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     7081 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     7141 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     7201 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     7261 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     7321 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     7381 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     7441 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     7501 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     7561 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     7621 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     7681 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     7741 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggggat
     7801 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     7861 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     7921 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     7981 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     8041 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     8101 tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt aagacttatc
     8161 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     8221 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     8281 taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct acgcaacttc
     8341 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     8401 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     8461 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     8521 tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc aggagataag
     8581 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     8641 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     8701 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     8761 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     8821 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac ggaacaggaa
     8881 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     8941 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     9001 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     9061 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     9121 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     9181 gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca taagctggac
     9241 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     9301 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     9361 gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg ttttgccgta
     9421 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     9481 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     9541 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     9601 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgtgcc
     9661 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     9721 cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt tagcgcaatg
     9781 tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac accttaagcg
     9841 ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt ttcccctcga
     9901 ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca aaactgccta
     9961 acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt tcgctgtgct
    10021 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
    10081 agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa actacgccgc
    10141 ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc ggcagcacaa
    10201 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat tgcaaatgcc
    10261 aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
    10321 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
    10381 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
    10441 accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag tacttcacag
    10501 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa tatggcgcgt
    10561 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
    10621 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
    10681 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
    10741 actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
    10801 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
    10861 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
    10921 accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc ggccaatggc
    10981 tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg taaactggtc
    11041 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
    11101 cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac cttcagcgcg
    11161 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
    11221 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
    11281 cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct ggaatatcgc
    11341 aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc aggagaagtg
    11401 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
    11461 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
    11521 ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
    11581 accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt ggtcgttcag
    11641 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
    11701 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
    11761 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgactgg
    11821 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
    11881 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
    11941 atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa ggaccgttat
    12001 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
    12061 aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa accaggagtc
    12121 actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac cgcctatacc
    12181 aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg ctggatttat
    12241 catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca ataatggtgt
    12301 actcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa
    12361 cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa cttccttcaa
    12421 caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc tgaaaagtag
    12481 taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat
    12541 cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt cgaaaaatga
    12601 agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg atgcaaaagg
    12661 caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg cgaaactgag
    12721 ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca gtttgaaaaa
    12781 tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc aacaggtgaa
    12841 ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag gtgatatcac
    12901 cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa atggcaaccc
    12961 actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta agttctcgat
    13021 tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct ccctgaccgg
    13081 cacgttagcg ggcacgcata tgatcatggc tcgtcgggct aacagcaatg tcagcgatgc
    13141 acagccaatg acgtttgtgg cggataaaga cagagcggtt gtcgttttgc aaacatcgaa
    13201 agcggaaatc attgggaatg gcgtggatga gacaactctg acagcaacag tgaaagatcc
    13261 gtcgaatcat ccggtggcgg ggataacggt aaacttcacc atgccacagg acgttgcggc
    13321 aaactttacc cttgaaaata acggtattgc catcactcag gccaatgggg aagcgcatgt
    13381 cacgctgaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgctgg gtaataacaa
    13441 taccagtgat tcgcagccgg taacatttgt ggcggacaaa gcctcggctc aggttgtcct
    13501 gcagatatca aaagatgaga tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac
    13561 ggttaaagat cagttcgaca atgaggtgaa taatcttccg gtaacattca gctcagcctc
    13621 ttcaggactc accctgaccc cgggagtaag taataccaat gagtctggca tcgcgcaggc
    13681 cactctcgca ggcgttgcct ttggtgagca gacggtcact gcatcactgg ctaataatgg
    13741 tgccagcgac aacaaaactg tgcattttat tggcgacaca gcggcggcaa aaattatcga
    13801 gttgacgcct gtcccagaca gcataatcgc cggtaccccg cagaacagct ccggcagcgc
    13861 catcaccgcc acagtcgttg ataataatgg ctttccggtg aaaggtgtga ctgtgaactt
    13921 caccagcaga acaaactctg ccgaaatgac gaatggcggc caagccgtaa cgaacgaaca
    13981 gggtaaggct accgtcactt ataccaatac ccgctcctcg atagaatcag gagcgagacc
    14041 ggataccgtt gaggccagtc tggaaaatgg tagctccacg cttagcacat caattaatgt
    14101 caacgctgat gcgtctacgg cacatctcac ctggctacag gcactttttg atacagtctc
    14161 cgcaggcgag acaaccagtc tgtatattga ggtgaaggat aattacggca acggtgtccc
    14221 ccagcatcag gtgaccctca gcgtttcacc aagtgaaggc gtgaccccca gtaataacgc
    14281 tatatatact accaaccacg acggcaattt ttacgcaagc tttaccgcta caaaagccgg
    14341 ggtttatcaa gtgacggcaa ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta
    14401 tgtgccgaac gtcacgaatg ccgaaatcac gctggcaacc tcgaaggatc cggtgattgc
    14461 cgacaataac gatctcacga cactaacagc aacagtcgct gatacagagg gcaatgcgat
    14521 agccaacact gaagtaacat ttactctgcc ggaagatgtt aaggcgaact tcacgctgag
    14581 cgatggcggt aaagcgatta ctgatgctga aggcaaagcg aaagtcacgc tgaaaggtac
    14641 aaaagcaggc gctcatactg ttacagcatc gataactggc ggtaagagtg agcagttggt
    14701 ggtgaacttt actgcggata cgctcactgc gcaggttaat cttaacgtta ccgaggacaa
    14761 ttttatcgct aataacgtcg ggatgaccag gctgcaggca acagtgactg atggaaacgg
    14821 caacccgtta gccaatgagg cggtgacatt cacgctaccg gcagatgtga gcgcaagctt
    14881 tactctcgga caaggcggtt ccgccattac tgacatcaac ggcaaggctg aagttacact
    14941 gagcggtaca aaatccggca cctaccccgt gacagttagc gtgaacaatt atggtgtcag
    15001 tgatacgaaa caggtgactt tgattgccga tgctggtacc gcaacactag cctccttaac
    15061 ctctgtatac tcattcgtcg tcagcacgac cgagggcgcg accatgactg caagcgtcac
    15121 tgacgctaac ggcaacccgg tagaaggcat aaaagttaat ttccgcggaa cctccgtcac
    15181 gctaagcagc accagcgttg aaacggatga tcggggtttc actgaaattc ttgtgacaag
    15241 caccgaagtc ggactgaaaa cagtttcagc ctctctggca gataaaccta ctgaagtcat
    15301 atcgcgatta ctgaatgcaa aagcagatat taattctgca acgattacca gtctggagat
    15361 acctgaaggt caggtcatgg tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca
    15421 gtttggcaac ccgattctta atgaatctgt aacattcagt gcagagccac cagagcacat
    15481 gaccatcagc caaaatattg tctctactga tacgcatggt atagccgagg tctccatgac
    15541 gcccgaaaga aacggttcgt atatggtgaa agcatccctg gcgaatggag cctcacttga
    15601 gaaacaactg gaggctattg atgaaaaact gacactctcg gcgtccagcc cgcttatcgg
    15661 tgtcaattcc ccaacaggtg caactctgac ggcaacgcta acttctgcaa atggcactcc
    15721 agtggagggt caggtcatca actttagcgt aacgccagaa ggtgcgacgt taagtggcgg
    15781 aaaagtgaga actaactctt ctggtcaggc tccagtcgtt ctgaccagca ataaagtcgg
    15841 tacatatacg gtgactgcat cgttccataa cggcgtaaca atacagacac agacaaccgt
    15901 gaaagtcact ggcaactcaa gcaccgccca tgttactagc tttatcgctg atccatcgac
    15961 tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg
    16021 taacctgatc gaaggtctca ctgtgtactt tgccttaaaa agcggctctg ccacattaac
    16081 gtcattaaca gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggagcgat
    16141 gacaggtagc gtcacggtaa gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat
    16201 aacgctggtg gcaggcccag cagacgcctc aaagtcagtc cttaagaaca atcggtcatc
    16261 attgaaagga gactttaccg atagtgctga gctacatctt gttctgcacg atatatcagg
    16321 caatccgatc aaagtttctg aagggctgga atttgtacag tcaggtacca acgtgcccta
    16381 tgtgcaagtt agtgcaattg actacagtaa aaatttctca ggcgagtaca aagccactgt
    16441 tacaggaggc ggagagggta tcacaacgct gatccctgta ttgaatggtg ttcatcaagc
    16501 tggtctgagt accacaatac aattcactcg cgcagaagac aaaataatga gcggtacagt
    16561 atcagtcaat ggtactgacc taccgacaac tacattccct tcgcaggggt tcaccggggc
    16621 gtattatcag ttgaataatg acaactttgc cccaggaaaa acggcggctg attatgagtt
    16681 ttcaagctct gcctcctggg tcgatgttga tgctaccggt aaagtgacat ttaaaaatgt
    16741 cggcagcaat tcggaaagga ttacggcgac accaaaatca ggaggcccta gctatgtata
    16801 cgaaatccgt gtgaagagtt ggtgggtgaa cgccggcgag gctttcatga tatacagcct
    16861 tgctgaaaat ttttgcagca gcaatggcta cacgctcccc agagcaaact atttaaacca
    16921 cagtagttcc cgaggcatcg ggtcactgta cagtgaatgg ggagatatgg ggcattacac
    16981 gactgaagct ggttttcaat caaatatgta ttggtcatct agtcccgcaa actcaagcga
    17041 acaatacgta gtttccctgg caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc
    17101 ttatgcgaca tgttataaaa acctctgatt ttccgttata atttcttaaa gagtgctcca
    17161 ttcggagctc tcttttttac attccctcta tagagcttgc ccttctctca cagaaaatga
    17221 ataaataaaa atgcggcacc gccagaattg cgttcgatgt gactatgatc gtatccggtg
    17281 agtactgctt tgattcaaag gctggttttc tgtgatgatt gaccagcctc agagtattga
    17341 tgacaccacc gttcataaca gatcggcatt actatgcata agtactgtct catattctcg
    17401 ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc
    17461 caacgacgac tgactccgag acatcggcca ttgcgggcca tcgcgttttg taatcagcat
    17521 aaattgcaac aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag
    17581 ttattgcgat ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtggtaagg
    17641 ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct tcacctttgg
    17701 gtccatgaac gatcactatt ggtatatctg tttcactttg aatttttgct attagatttt
    17761 ctgcaatcga taatgaaaat gtacgttcct gcgagctacc ttctaaattg aacgcaatgt
    17821 aagatcctaa cgatcgcatt tcctcgcgca cctcatcgag tacatcctca cttagtggca
    17881 attcatatat tggcctgact gctggaaaac ccgcctcacg catcataaat gcccatgtca
    17941 taggtacggg agcccggagt ttctgatcca tacgggacgc gttcttgcac aaaggggagt
    18001 agcaattcat ggttatacca acaacctgaa aattcgtttt tgctttcaac tgactgataa
    18061 ataacatcgt tttcaggttc tttttacgca tcccctcaat acaaagatcc ggcgtaccgt
    18121 attgctgtgt tatgttcttt gctaaatctt ttatttcttt taatgttgcg tgatcctgca
    18181 tagtcattgt gactaatgtt aatttagtct gttcaagttt aagcgcatta aagacttcta
    18241 aattaattgt cgacgttaca attaaaagat gcttaatttt atgcaattca agcgcccgaa
    18301 taacaggaaa gatggccata gcatcgccaa tctgatcggg aatatggatg acaacaaagt
    18361 ctgttttttc aatattgaaa ttataagctt tataatcgta gtaactaaat gcaatacgtc
    18421 tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata atgcaatgca
    18481 gtctatcaga ctcatcaggg tgccattttg tgcatatgcg gacttttatg tttcatatct
    18541 ctaacctgtg ggtcctctgc ttaatcctta aacaacacca gcaactcctg cgctttcatc
    18601 ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct
    18661 gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt accttttcca
    18721 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
    18781 cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagcttc
    18841 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc cgcactggtg
    18901 tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgct
    18961 acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc
    19021 gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
    19081 gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttgctg
    19141 gtgacactgc gtgtcattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg
    19201 ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
    19261 ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg
    19321 actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg
    19381 gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
    19441 caacattatc aagctgccgc gaaaaaacgc atcccggtta tcgaagcgct gttacgacat
    19501 cccggtgctt tcctgaagat tattgcgcta cgactgtgcg aattgctgac gatgtacatc
    19561 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggctaccgcg cgaacttttc
    19621 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
    19681 cttgccgatc gttttggtcg ccgtagggtt tatatcacag gtgcgttaat cggaacgttg
    19741 agcgcatttc ctttctttat ggcg
//
LOCUS       DAZPBM010000072        19530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPBM010000072 DAZPBM010000000
VERSION     DAZPBM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.072"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHUZQ_002299"
     CDS             <1..245
                     /locus_tag="ACHUZQ_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR1025982.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUZQ_002300"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUZQ_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1025983.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUZQ_002301"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUZQ_002301"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR1025984.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUZQ_002302"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUZQ_002302"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR1025985.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHUZQ_002303"
     CDS             3423..4061
                     /locus_tag="ACHUZQ_002303"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1025986.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHUZQ_002304"
     CDS             4066..4842
                     /locus_tag="ACHUZQ_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR1025987.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHUZQ_002305"
     CDS             4931..6295
                     /locus_tag="ACHUZQ_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR1025988.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUZQ_002306"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUZQ_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1025989.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUZQ_002307"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUZQ_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1025990.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUZQ_002308"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUZQ_002308"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1025991.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUZQ_002309"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUZQ_002309"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1025992.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUZQ_002310"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUZQ_002310"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1025993.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUZQ_002311"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUZQ_002311"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1025994.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUZQ_002312"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUZQ_002312"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1025995.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUZQ_002313"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUZQ_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1025996.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHUZQ_002314"
     CDS             complement(12844..13167)
                     /locus_tag="ACHUZQ_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1025997.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUZQ_002315"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUZQ_002315"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1025998.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUZQ_002316"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUZQ_002316"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1025999.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUZQ_002317"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUZQ_002317"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1026000.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHUZQ_002318"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHUZQ_002318"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1026001.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHUZQ_002319"
     CDS             complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHUZQ_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR1026002.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHUZQ_002320"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHUZQ_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHUZQ_002321"
     CDS             complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHUZQ_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1026003.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4605 a         5270 c         4896 g         4759 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gcccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaagcg
//
LOCUS       DAZPBM010000073        19481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPBM010000073 DAZPBM010000000
VERSION     DAZPBM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.073"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            376..825
                     /gene="alaE"
                     /locus_tag="ACHUZQ_002322"
     CDS             376..825
                     /gene="alaE"
                     /locus_tag="ACHUZQ_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1026004.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(862..1206)
                     /locus_tag="ACHUZQ_002323"
     CDS             complement(862..1206)
                     /locus_tag="ACHUZQ_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1026005.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1358..1687
                     /locus_tag="ACHUZQ_002324"
     CDS             1358..1687
                     /locus_tag="ACHUZQ_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR1026006.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1935..2180
                     /gene="nrdH"
                     /locus_tag="ACHUZQ_002325"
     CDS             1935..2180
                     /gene="nrdH"
                     /locus_tag="ACHUZQ_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1026007.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2177..2587
                     /gene="nrdI"
                     /locus_tag="ACHUZQ_002326"
                     /pseudo
     CDS             2177..2587
                     /gene="nrdI"
                     /locus_tag="ACHUZQ_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2560..4704
                     /gene="nrdE"
                     /locus_tag="ACHUZQ_002327"
     CDS             2560..4704
                     /gene="nrdE"
                     /locus_tag="ACHUZQ_002327"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1026008.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4714..5673
                     /gene="nrdF"
                     /locus_tag="ACHUZQ_002328"
     CDS             4714..5673
                     /gene="nrdF"
                     /locus_tag="ACHUZQ_002328"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1026009.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6029..7231
                     /gene="proV"
                     /locus_tag="ACHUZQ_002329"
     CDS             6029..7231
                     /gene="proV"
                     /locus_tag="ACHUZQ_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1026010.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7224..8288
                     /gene="proW"
                     /locus_tag="ACHUZQ_002330"
     CDS             7224..8288
                     /gene="proW"
                     /locus_tag="ACHUZQ_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1026011.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8346..9338
                     /gene="proX"
                     /locus_tag="ACHUZQ_002331"
     CDS             8346..9338
                     /gene="proX"
                     /locus_tag="ACHUZQ_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1026012.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9629..10813
                     /locus_tag="ACHUZQ_002332"
     CDS             9629..10813
                     /locus_tag="ACHUZQ_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1026013.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10937..11674
                     /gene="ygaZ"
                     /locus_tag="ACHUZQ_002333"
     CDS             10937..11674
                     /gene="ygaZ"
                     /locus_tag="ACHUZQ_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1026014.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11664..11999
                     /gene="ygaH"
                     /locus_tag="ACHUZQ_002334"
     CDS             11664..11999
                     /gene="ygaH"
                     /locus_tag="ACHUZQ_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1026015.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12090..12620
                     /gene="emrR"
                     /locus_tag="ACHUZQ_002335"
     CDS             12090..12620
                     /gene="emrR"
                     /locus_tag="ACHUZQ_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1026016.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12747..13919
                     /gene="emrA"
                     /locus_tag="ACHUZQ_002336"
     CDS             12747..13919
                     /gene="emrA"
                     /locus_tag="ACHUZQ_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR1026017.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13936..15474
                     /gene="emrB"
                     /locus_tag="ACHUZQ_002337"
     CDS             13936..15474
                     /gene="emrB"
                     /locus_tag="ACHUZQ_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1026018.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15538..16053)
                     /gene="luxS"
                     /locus_tag="ACHUZQ_002338"
     CDS             complement(15538..16053)
                     /gene="luxS"
                     /locus_tag="ACHUZQ_002338"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1026019.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16203..17759)
                     /gene="gshA"
                     /locus_tag="ACHUZQ_002339"
     CDS             complement(16203..17759)
                     /gene="gshA"
                     /locus_tag="ACHUZQ_002339"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1026020.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17832..18260)
                     /locus_tag="ACHUZQ_002340"
     CDS             complement(17832..18260)
                     /locus_tag="ACHUZQ_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1026021.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18257..18823)
                     /gene="yqaB"
                     /locus_tag="ACHUZQ_002341"
     CDS             complement(18257..18823)
                     /gene="yqaB"
                     /locus_tag="ACHUZQ_002341"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1026022.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19104..19180)
                     /locus_tag="ACHUZQ_002342"
     tRNA            complement(19104..19180)
                     /locus_tag="ACHUZQ_002342"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19144..19146),aa:Arg,seq:acg)
     gene            complement(19379..19455)
                     /locus_tag="ACHUZQ_002343"
     tRNA            complement(19379..19455)
                     /locus_tag="ACHUZQ_002343"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19419..19421),aa:Arg,seq:acg)
BASE COUNT         4638 a         5034 c         4915 g         4894 t
ORIGIN      
        1 actggtgctt tctgctgtta atgtacattt tctggcatca aaaatgctgt aaatgacacg
       61 gtcacgacat acaccgaatt ctctacgctt atagcgttac ctcaccccca aactttcatt
      121 agtgaaatta aaggcaaggt catatctaat tgatttaatt gaaaataaat catatcggca
      181 tgtaaaaatc cacaatgtac aaaaaacgag ccgttacgga atattttatc tacaaaaact
      241 gactaaataa aaatttttca ctaattgaat agtcatagcc agcgatatac gctatgcgaa
      301 aatgcagatg gcaatgagat ccactgcttt catctccatt aacatcccat tacgctttta
      361 ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc gtcatgcagt tgcagatacg
      421 ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta ttgaagtttt cctctccgga
      481 atgagcttcg aacagtcttt ttattccaga ttggtagcga ttccggtgaa catcttaatt
      541 gcatggccat acggtatgta ccgcgatcta tttatgcgcg cggcacgcaa agttagcccg
      601 tcgggctgga taaaaaatct ggctgatatc ctggcttatg tgacgttcca gtcaccggtg
      661 tatgtggcga tcttgttagt ggtgggcgca gactggcatc agattatggc ggcggtcagt
      721 tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg gctacttcct cgattattgc
      781 cgccgactgt ttaaagtcag ccattaccag caggtaaaag cctgactctt cttttcgcga
      841 ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat aaaccattca aataacgttc
      901 aagcgcaaca cgcgaactga agccatgcgg gataccgtaa tgctcgtgtc gctcgcctgc
      961 taaatagagc ggaaattgtt gataatgatc gcaagtttta atatcggaag cgggttcagc
     1021 cagtgtcgaa ctacgttctt ttaatgttgg atgaattacc agtgcggtac gtcccattcg
     1081 cgcttcgcga ttaacataga cataattttc cccacggcgg taaccataag ctttctgggt
     1141 taccacatcg acagtaaaac cgactttttc aagtacgcgc gccacctcgt ctggtcgtaa
     1201 atacatattt gatcctcgtt attatcaacc gcgggcctac cttacctgat tgcgcattca
     1261 caacgctttc agaaaagtcc ataaagccgc gatgaacctc agttaagaaa attatggtct
     1321 acactgaaaa ttacatcgaa ttctaatggg ggatcatatg tttaaccgac cgaaccgcaa
     1381 cgacgttgat gatggcgtgc aggatatcca gaatgatgtc aatcaattag ctgacagcct
     1441 cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg gaagctgaag ccgcacgcag
     1501 taaagctcag gcattgctga aagaaaccag ggcgcgaatg catggtcgta ctcgcgtcca
     1561 gcaagccgca cgcgatgccg ttggctgcgc ggattctttt gttcgtgaaa gaccctggtg
     1621 tagcgtgggt acagcagctg cggtaggtat ttttatcggc gcactgttaa gcatgcgcaa
     1681 atcgtagtgc aaaaatgata ataaatacgc gtctttaacc ccgaagcctg tcttcggggt
     1741 ttctttttgc ctggtgaatc acaaaaatcc ccctcccccg tcacgctcat atctagggta
     1801 atttcgatca ctatttgcta tatattgtgt ggttgaatct tttttcaact acatctagta
     1861 tctctgtatc aacagagaga caacccgacg cgtatcatcg cgccgtatct tcattttaaa
     1921 cggaaatacg aatcatgcgc ataactattt acactcgtaa cgattgcgtt cagtgccacg
     1981 ccaccaaacg ggcgatggaa aaccggggct ttgattttga aatgattaat gtcgatcgcg
     2041 ttcctgaagc agcggaagcg ttgcgtgctc agggctttcg tcagttgccg gtagtgattg
     2101 ctggcgatct tagctggtct ggtttccgtc cggacatgat taaccgtctg catccagcgc
     2161 cacacgcggc cagtgcatga gccagctcgt ctacttctcc agcagctccg aaaacacgca
     2221 gcgttttatc gaacgtttag gtctgcccgc ggtgcgcatc ccgctcaatg agcgggaacg
     2281 gattcaggta gacgagcctt acatcctgat cgtgccctct tacggcggcg gcggtacggc
     2341 tggcgcggtg ccacgatagg taattcgctt tttaaacgac gagcacaacc gggcgttgct
     2401 tcgcggcgtt attgcttctg gtaatcgcaa ctttggtgag gcgtatggcc gcgccggaga
     2461 tgtgattgcc cggaaatgcg gcgtgccgtg gctgtaccgt tttgaactca tgggtacgca
     2521 aagcgatatc gaaaacgttc gtaaaggagt aaccgaattt tggcaacgac aaccgcagaa
     2581 tgcctgacgc aggaaacgat ggattaccac gcgctgaatg cgatgcttaa cctctacgat
     2641 agcgcaggtc gcattcagtt cgataaagac cgccaggccg ttgacgcctt tatggcgacg
     2701 catgtgcgtc cgaacagtgt gaccttcagt agccagcagc agcgcctgaa ctggctggtc
     2761 aacgaaggtt actatgatga aagcgttctt aatcgctact ctcgcgactt tgtcattacg
     2821 ctgtttgccc acgcacacac cagcggtttt cgtttccaga cattcctcgg ggcatggaag
     2881 ttttacacca gctatacgtt gaagacattc gacggtaaac gttatctgga agattttgcc
     2941 gatcgagtca cgatggtggc gctgacgctg gcacaaggcg atgcgacgct ggcgttgcaa
     3001 ctgaccgatg aaatgctgtc aggacgcttt cagccagcca cgccgacatt cctcaactgc
     3061 ggtaagcagc agcgcggcga actggtttcc tgttttctgc tgcgtattga agacaatatg
     3121 gagtcgattg gccgggcggt aaattccgca ctgcaactgt cgaaacgcgg cggcggcgtg
     3181 gcatttttgc tgtcgaatct gcgagaagcg ggcgcgccaa ttaaacgtat tgaaaatcaa
     3241 tcttctggcg taattccggt gatgaaaatg ctggaagacg cattttccta tgccaaccaa
     3301 ctcggcgcgc gtcagggggc aggtgcagtc tatttacatg ctcatcatcc cgatattctg
     3361 cgttttctcg atacaaaacg ggaaaatgcc gacgaaaaaa tccgcattaa aacactgtcg
     3421 ctgggcgtgg tgatcccgga tatcactttc catctggcaa aagagaatgc gcagatggcg
     3481 ctgttttcgc cctatgacgt tgagcgagtt tatggcaagc cgtttgccga tatcgccatc
     3541 agcgaacact atgacgaact ggttgccgat gaacgcatcc gcaaaaaata cctcaacgcc
     3601 cgtgatttct tccagcggct ggcagaaatc cagtttgagt ccggctatcc ctacatcatg
     3661 tatgaagaca cggtaaaccg tgctaaccct gtcgccgggc gcataaatat gagtaatctc
     3721 tgctcagaga ttttgcaggt caactgtgcc tcagagtatg acgagaatct cgactatgcc
     3781 cgcacaggcc atgatatctc ctgcaattta ggttcgttga atattgcgca caccatggat
     3841 tcccccgatt ttgcccgcac ggtagagact gccgtgcgcg gtttaacggc ggtgtcagat
     3901 atgagtcata tccgcagcgt gccgtccatc gaagccggaa atgccgcctc acacgccatc
     3961 ggactggggc agatgaattt acacggctat ctggcgcgag aaggcatcgc ttatggttcg
     4021 ccggaagcac tggatttcac caatctctat ttctatgcca tcacctggca cgcactgcgt
     4081 acctcgatgt tgctggcgcg cgaacgcggt gaaaccttcg ccgggttcaa acagtcacgt
     4141 tatgccagtg gtgaatattt tagccaatat ctgcaaggta actggcagcc gaaaacggcg
     4201 aaagttggcg aactgtttgc ccgcagcggt attacgttac ctacccgtga gatgtgggcg
     4261 caactgcgcg acgacgtgat gcgctacggc atttacaacc agaatcttca ggcggtgccg
     4321 ccaaccggtt ctatctctta tatcaaccat gctacgtcga gtattcatcc gattgtggcg
     4381 aaagtagaga tacgcaaaga gggcaaaaca ggacgcgttt actaccctgc cccgtttatg
     4441 actaacgaaa atctggcgct gtatcaggat gcttacgaaa tcggcgcaga aaagatcatc
     4501 gacacctacg cggaagcgac tcgccatgtc gatcagggtt tgtcgctgac gctgtttttc
     4561 cccgataccg ccaccactcg cgatatcaac aaagcgcaga tttacgcctg gcgcaaaggt
     4621 atcaaaacgc tctattacat tcgcctgcgt cagatggcgc tggaaggcac tgaaattgaa
     4681 ggctgcgtct cctgtgcact ttaaggaata tctatgaaac tctcacgtat cagcgccatc
     4741 aactggaaca agatatctga cgataaagat ctggaggtgt ggaatcgcct gaccagcaat
     4801 ttctggctac cagaaaaggt gccgctgtcg aacgatattc ctgcctggca gacattaact
     4861 gtcgtagaac aacaactgac gatgcgcgtt tttactggcc tgacgctgct cgacacgctg
     4921 caaaatgtta tcggcgcgcc ttcgctgatg cccgatgcac tcacgcctca tgaagaagcg
     4981 gtattatcga atatcagctt tatggaagcg gttcatgccc gctcttacag ttcgattttc
     5041 tcgacgctgt gccagaccaa agatgtcgat gccgcctacg cctggagtga agaaaacgca
     5101 ccgttgcagc gaaaagctca gattattcag caacattatc gcggtgatga tccgctgaaa
     5161 aagaaaatcg ccagtgtgtt tcttgaatct tttttgttct attccggttt ctggctgccg
     5221 atgtattttt ccagccgcgg aaagctgacc aataccgcag acctgatccg tctgattatc
     5281 cgtgatgaag cagtccacgg ttactacata ggctataaat atcagaaaaa catggaaaag
     5341 atatctctgg gacaacgtga agagttgaag agtttcgcct tcgatttgtt gctggaactc
     5401 tacgacaacg agctgcaata caccgatgag ctgtacgccg aaaccccgtg ggctgacgat
     5461 gtgaaagcgt ttctctgtta caacgccaat aaggctttga tgaatctggg ctacgaacct
     5521 ttatttcccg cagaaatggc ggaagtgaat ccggcaatcc tcgccgcgct ttcgccgaat
     5581 gccgatgaaa atcacgattt cttttccggt tcaggctcct cttatgtgat ggggaaagcg
     5641 gttgaaacag aagatgaaga ctggaatttc tgagggtgtt attttcaaaa atatcactac
     5701 ccgcgacagg gaaataattc ccgccaaata gctttttatc acgcaaatat tttgtggtga
     5761 tctacactga tactctgttg cattattcgc ctgaaaccac aatattcagg cgttttttcg
     5821 ctatctttga caaaaaatat caacttttct cgatttgctc tcagccctta tatcacggga
     5881 aattccggcg atttgctcgc atcaatattc atgccacatt tgccatcagg ggttgcctca
     5941 gattctcagt atgttagggt agaaaaaagt aactatttcc attgggtaat atatcgacat
     6001 aaacaaataa caggaatctt tctattgcat ggcaattaaa ttagaaatta aaaaccttta
     6061 taaaatattt ggtgagcatc cacagcgagc attcaaatat atcgaacaag gactttcaaa
     6121 agaacaaatt ctggaaaaaa ctgggctatc gcttggcgta aaagacgcca gtctggccat
     6181 tgaagaaggc gagatatttg tcatcatggg attatccggc tcgggtaaat ccacaatggt
     6241 acgccttctc aatcgcctga ttgaacccac ccgcgggcaa gtgctgattg atggtgtgga
     6301 tattgccaaa atatccgatg ccaaactccg tgaggtgcgc agaaaaaaga ttgcgatggt
     6361 cttccagtcc tttgccttaa tgccgcatat gaccgtgctg gacaatactg cgttcggtat
     6421 ggaattggcc ggaattaatg ccgaagaacg ccgggaaaaa gcccttgatg cactgcgtca
     6481 ggtcgggctg gaaaattatg cccacagcta cccggatgaa ctctctggcg ggatgcgtca
     6541 acgtgtggga ttagcccgcg cgttagcgat taatccggat attttattaa tggacgaagc
     6601 cttctcggcg ctcgatccat taattcgcac cgagatgcag gatgagctgg taaaattaca
     6661 ggcgaaacat cagcgcacca ttgtctttat ttcccacgat ctcgatgaag ccatgcgtat
     6721 tggcgaccga attgccatta tgcaaaatgg tgaagtggta caggtcggca caccggatga
     6781 aattctcaat aatccggcga atgattatgt ccgtaccttc ttccgtggcg ttgatattag
     6841 tcaggtattc agtgcgaaag atattgcccg ccggacaccg aatggcttaa ttcgtaaaac
     6901 ccctggcttc ggcccacgtt cggcactgaa attattgcag gatgaagatc gcgaatatgg
     6961 ctacgttatc gaacgcggta ataagtttgt cggcgcagtc tccatcgatt cgcttaaaac
     7021 cgcgttaacg cagcagcaag gtcttgatgc ggcgctgatt gatgcgccgt tagcagtcga
     7081 tgcacaaacg cctcttagcg agttgctctc tcatgtcgga caggcaccct gtgcggtgcc
     7141 cgtggtcgac gaggaccaac agtatgtcgg catcatttcg aaaggaatgc tgctgcgcgc
     7201 tttagatcgt gagggggtaa ataatggctg atcaaaataa tccgtgggat accacgccag
     7261 cggcggacag tgccgcgcaa tccgcagacg cctggggtac accgacgact gcaccgactg
     7321 acggcggtgg tgctgactgg ctgaccagta cgcctgcgcc aaacgtcgag cattttaata
     7381 ttctcgatcc gttccataaa acgctgatcc cgctcgacag ttgggtcact gaagggatcg
     7441 actgggttgt tacccatttc cgtcccgtct tccagggcgt gcgcgttccg gttgattata
     7501 tcctcaacag tttccagcaa ttgctgctgg gtatgccagc gccagtggcg attatcgttt
     7561 tcgctctcat cgcctggcag atttccgggg tcggaatggg cgtggcgacg ctggtttcgc
     7621 tgattgccat cggcgcaatc ggtgcctggt cgcaggcaat ggtgactctg gcgctggtgt
     7681 taaccgccct gctgttctgt atcgtcatcg gtttgccgtt ggggatatgg ctggcgagaa
     7741 gtccgcgagc ggcgaaaatt attcgtccac tgcttgatgc catgcagacc acgccagcgt
     7801 ttgtttatct ggtaccaatc gtcatgctgt ttggtatcgg taacgtgccg ggcgtagtgg
     7861 tgacgatcat ctttgctctg ccgccgatta tccgtctgac gattctgggg attaaccagg
     7921 ttccggcgga tctgattgaa gcctcgcgct cattcggtgc cagcccgcgc cagatgctgt
     7981 tcaaagttca gttaccgctg gcgatgccga ccattatggc gggcgttaac cagacgctga
     8041 tgctggctct ttctatggtg gtcatcgcct cgatgattgc cgtcggcggg ctgggtcaga
     8101 tggtacttcg cggtatcggt cgtctggata tgggccttgc caccgttggc ggcgtcggga
     8161 ttgtgatcct cgccattatc ctcgaccgcc tgacgcaggc cgttgggcgc gactcacgca
     8221 gtcgcggcaa ccgtcgctgg tacaccactg gccctgtcgg tctgctgacc cgcccattca
     8281 ttaagtaact ctgcacttgc ccggtaacgc cgggcattat caccctgcca aaaaaaggaa
     8341 taacaatgcg acatagcgta ctttttgcga cagcgtttgc cacgcttatc tctacacaaa
     8401 cttttgcagc cgatctgccg ggcaaaggca ttactgttaa tccagttcag agcaccatca
     8461 ctgaagaaac cttccagacg ctgctggtca gtcgtgcact ggagaaatta ggttataccg
     8521 tcaataaacc cagcgaagtg gattacaacg ttggctacac ctcacttgct tccggcgatg
     8581 caaccttcac cgccgtgaac tggacgccgc tgcatgacaa catgtacgaa gctgccggtg
     8641 gcgataagaa attttattgt gaaggggtat tcgttaacgg cgcggcacag ggttacctga
     8701 tcgataagaa aaccgccgac cagtacaaaa tcaccaacat cgcacaactg aaagatccga
     8761 agatcgccaa actgttcgat accaacggcg acggtaaagc ggatttaacc ggctgtaacc
     8821 caggctgggg ctgcgaaggt gcgatcaacc accagcttgc cgcgtatgaa ctgaccaaca
     8881 ccgtgacgca taatcagggg aactacgcag cgatgatggc cgacaccatc agtcgctaca
     8941 aagagggcaa accggtgttt tattacacct ggacgccgta ctgggtgagt aatgagctga
     9001 agccagggaa agatgtggtc tggttgcagg tgccgttctc cgcactgccg ggcgataaaa
     9061 acgccgatac caaactgccg aatggtgcga attatggctt cccggtcagc accatgcata
     9121 tcgttgccaa caaagcctgg gccgagaaaa acccggcagc agcgaaactt tttgccatta
     9181 tgcagttgcc agtggcagat attaacgccc agaacgccat tatgcatgac ggcaaagcct
     9241 cagaaggcga tattcagggc catgttgatg gttggatcaa agcccaccag cagcagttcg
     9301 atggctgggt gaatgaggcg ctggcagcgc agaagtaatt tttattcggg cggataagtc
     9361 gtttttgcct catccgccat tctgtgcaca atgcctgatg cgacgctggc gcgtcttatc
     9421 atgcctacaa aatacactca ttccgtatgg cggataaggc gttttcgccg catccgccgt
     9481 tctgtgcaca atgcctgatg cgacgctggc gcgtcttatc atgcctacaa accacatcgc
     9541 acatttcaac aagctatctt tcgcccccat tcatcaacat ccgctattat tgatttccag
     9601 cttaatcatc acctgatgaa caaaaataat gactaaacct aatcatgagc ttagcccggc
     9661 gctgatcgtg ctgatgtcta tcgccaccgg tcaggcggta gccagtaact attacgccca
     9721 gccattgctc gacaccatcg cgcgtaactt ttccctttcc gccagttcgg caggctttat
     9781 tgttaccgcc gcgcagttgg gctatgcagc aggtctgctg tttcttgttc ccctcggtga
     9841 tatgtttgaa cgccgccgcc tgattgtctc gatgacctta ctggcggcag gcggcatgtt
     9901 aatcactgcc agcagtcagt cgctggcgat gatgatcctc ggtacggcat taaccggttt
     9961 attctcagtc gtggcacaaa ttctggttcc gctggcagcg acgctggctt caccggataa
    10021 acgcggcaaa gtggttggca ccattatgag cggtctgctg ttggggatct tgctggcacg
    10081 aaccgttgcc gggttactgg caaacctcgg cggctggcgc accgtctttt gggttgcctc
    10141 ggtgttaatg gcactgatgg cgctagcatt atggcgtggt ctgccacaaa tgaaatcaga
    10201 aacccacctc aactacccac agttgttagg ttccgttttc agcatgttta tcagcgataa
    10261 gatcctccgc acccgcgcat tgctgggctg cctgaccttt gccaacttca gcattctctg
    10321 gacctcaatg gcctttttgc ttgccgctcc accttttaac tacagcgatg gtgtcattgg
    10381 tctgtttgga cttgcgggag ctgccggagc gttgggcgct cgtccggcgg gcggttttgc
    10441 cgataagggt aaatcgcacc acaccacaac tttcggtctg ctgctgctat tactttcatg
    10501 gctggcgatc tggtttggtc acacttccgt actggcgctg attatcggca tcctggtgct
    10561 ggatctcacc gtacagggcg tgcatatcac taaccagacg gtaatttatc gaatacaccc
    10621 tgatgcgcgt aatcgcctga ccgcaggtta catgaccagc tactttattg gcggtgccgc
    10681 cggttcgcta atttcagcct cagcctggca acatggcggt tgggctggcg tttgtctggc
    10741 tggcgcgacg attgccctag ttaacttact ggtctggtgg cgaggttttc atcgtcagga
    10801 agccgcaaat taagcaatca gcaaccgttt tcgtggcttt acacttataa gggtgttaag
    10861 aagcccatca gtctgataag gttaagatat tcattcagtc tatttataat attaacaatc
    10921 gttaagcgta cactctatgg aaagccctac tccacagcct gctcctggtt cggcgacctt
    10981 catggaagga tgcaaagaca gtttaccgat tgttattagt tatattccgg tggcctttgc
    11041 gttcggtctg aatgcgaccc gtctgggatt ctctcctctc gaaagcgttt ttttctcctg
    11101 catcatttat gcaggcgcga gccagttcgt cattaccgcg atgctggcag ccgggagtag
    11161 tttgtgggtt gctgcactga ccgtcatggc aatggatgtt cgccatgtgt tgtatggccc
    11221 gtcactgcgt agccgtatta ttcagcgtct gcaaaaatcg aaaaccgccc tgtgggcgtt
    11281 tggcctgacg gatgaggttt ttgccgccgc aacagcaaaa ctggtacgca ataatcgccg
    11341 ctggagcgag aactggatga tcggcattgc cttcagttca tggtcatcgt gggtatttgg
    11401 tacggtaata ggggcattct ccggcagcgg cttgctgcaa ggttatcccg ccgttgaagc
    11461 agcattaggt tttatgcttc cggcactctt tatgagtttc ctgctcgcct ctttccagcg
    11521 caaacaatct ctttgcgtta ccgcagcgtt agttggtgcc cttgcaggcg taacgctatt
    11581 ttctattccc gtcgccattc tggcaggcat tgtctgtggc tgcctcactg cgttaatcca
    11641 ggcattctgg caaggagcgc ccgatgagct atgaggttct gctgcttggg ttactggttg
    11701 gcgcggcgaa ttattgcttc cgctatttgc cgctgcgcct gcgtgtgggt aatgcccgcc
    11761 caaccaaacg tggcgcggta ggtattttgc tcgacaccat tggcatcgcc tcgatatgcg
    11821 ctctgctggt tgtctctacc gcaccagaag tgatgcacga tacacgccgt ttcgtgccca
    11881 cgctggtcgg cttcgcggta ctgggtgcca gtttctataa aacacgcagc attatcatcc
    11941 caacactgct tagtgcgctg gcctatgggc tcgcctggaa agtgatggcg attatataac
    12001 ccacaagaat catttttcta aaacaataca tttactttat ttgtcactgt cgttactata
    12061 tcggctgaaa ttaatgaggt catacccaaa tggatagttc gtttacgccc attgaacaaa
    12121 tgctaaaatt tcgcgccagc cgccacgaag attttcctta tcaggagatc cttctgactc
    12181 gtctttgcat gcacatgcaa agcaagctgc tggagaaccg caataaaatg ctgaaggctc
    12241 aggggattaa cgagacgttg tttatggcgt tgattacgct ggagtctcag gaaaaccaca
    12301 gtattcagcc ttctgaatta agttgtgctc ttggatcatc ccgtaccaac gcgacgcgta
    12361 ttgccgatga actggaaaaa cgcggttgga tcgaacgtcg tgaaagcgat aacgatcgcc
    12421 gctgcctgca tctgcaatta acggaaaaag gtcacgagtt tttgcgcgag gttttaccac
    12481 cgcagcataa ctgcctgcat caactctggt ccgcgctcag cacaacagaa aaagatcagc
    12541 tcgagcaaat cacccgcaaa ttgctctccc gtctcgacca gatggaacaa gacggtgtgg
    12601 ttctcgaagc gatgagctaa cgcgtcatct cgctcaaaaa tccagattta taaaagaaaa
    12661 aatgaccggc cagcatcgca acatgctggc ctttttggca agcaggtcgg ctcagccgat
    12721 gagttaagaa gatcgtggag aacaatatga gcgcaaatgc ggagactcaa accccgcagc
    12781 aaccggtaaa gaagagcggc aaacgtaagc gtctgctcct ccttctcacc ttgctcttta
    12841 taattattgc cgtagcgata gggatttatt gttttttggt actgcgtcat ttcgaagaaa
    12901 ccgatgacgc atacgtggca gggaatcaga ttcaaattat gtctcaggtg tctggcagcg
    12961 tgacgaaagt ctgggccgat aacaccgatt ttgtaaaaga aggcgacgtg ctggtcactc
    13021 tcgacccgac agatgctcgc caggcgtttg aaaaagccaa aactgcactg gcttccagcg
    13081 ttcgccaaac ccaccagctg atgattaaca gcaaacagtt gcaggcgaat attgaggtgc
    13141 agaaaatcgc cctcgcaaaa gcacaaagcg actacaaccg ccgtgtgccg ctgggcaatg
    13201 ccaacctgat tggtcgcgaa gagctgcaac acgcccgcga cgccgtcacc agtgcccagg
    13261 cgcaactgga cgtcgctatt caacaataca atgccaatca ggcgatgatt ctggggacta
    13321 aactggaaga tcagcctgcc gtgcaacagg ctgccaccga agtacgtaac gcctggctgg
    13381 cgctggagcg tacccgtatt gtcagtccga tgaccggtta tgtctcccgc cgcgcggtac
    13441 agcctggggc gcaaattagc ccaacgacgc cgctgatggc ggtcgttcca gccaccaata
    13501 tgtgggtgga tgccaacttt aaagagacgc agattgccaa tatgcgtatc ggtcagccgg
    13561 tcactatcac cacggatatt tacggcgatg atgtgaaata caccggtaaa gtggttggtc
    13621 tggatatggg cacaggtagc gcattctcac tgcttccggc gcaaaatgcg accggtaact
    13681 ggatcaaagt cgttcagcgt ctacctgtgc gtatcgaact ggaccagaaa cagctggagc
    13741 aatacccgct gcgcattggt ttgtccacac tggtgagcgt caataccact aaccgtgacg
    13801 gtcaggtact ggcaaataaa gtacgttcaa caccggtcgc ggtaagtacc gcgcgtgaaa
    13861 tcagcctggc acctgtcaat aaactgatcg acgatatcgt aaaagctaac gctggctaat
    13921 ccagaggtgc gtgtgatgca acagcaaaaa ccgctggaag gcgcgcaact ggtcattatg
    13981 acgattgcgc tgtcactggc gacattcatg caggtgctgg actccaccat tgctaacgtg
    14041 gcgatcccca ctatcgccgg gaatctgggc tcatcgctca gccagggaac gtgggtaatc
    14101 acttctttcg gggtggcgaa tgccatctcg atcccgctta ccggctggct ggcaaagcgc
    14161 gtcggggaag tgaaactgtt cctttggtcc accatcgcct ttgctattgc gtcgtgggcg
    14221 tgtggtgtct ccagcagcct gaatatgctg atcttcttcc gcgtgattca ggggattgtc
    14281 gccgggccgt tgatcccgct ttcgcaaagt ctattgctga ataactaccc gccagccaaa
    14341 cgctcgatcg cgctggcgtt gtggtcgatg acggtgattg tcgcgccaat ttgcggcccg
    14401 atcctcggcg gttatatcag cgataattac cactggggct ggatattctt catcaacgtg
    14461 ccgattggcg tggcggtggt gttgatgaca ctgcaaactc tgcgcggacg tgaaacccgc
    14521 accgaacggc ggcggattga tgccgtgggg ctggcactgc tggttattgg tatcggcagc
    14581 ctgcagatta tgctcgaccg cggtaaagag ctggactggt tttcatcaca ggaaattatc
    14641 atccttaccg tggtggcggt ggtggctatc tgcttcctga ttgtctggga gctgaccgac
    14701 gataacccga tagtcgatct gtcgttgttt aagtcgcgca acttcaccat cggctgcttg
    14761 tgtatcagcc tcgcgtatat gctctacttc ggcgctattg ttctgctgcc gcagttgttg
    14821 caggaggtct acggttacac ggcgacctgg gcaggtttgg cctctgcgcc ggtagggatt
    14881 attccggtga tcctgtcgcc gattatcggc cgcttcgcgc ataaactgga tatgcggcgg
    14941 ctggtaacct tcagctttat tatgtatgcc gtctgcttct actggcgtgc ctataccttt
    15001 gaaccaggta tggattttgg cgcgtcggcc tggccgcagt ttatccaggg gtttgcggtg
    15061 gcctgcttct ttatgccgct gaccaccatt acgctgtctg gtttgccacc ggaacgactg
    15121 gcggcggcat cgagcctctc taactttacg cgaacgctgg cggggtctat cggcacgtcg
    15181 ataaccacga ccatgtggac caaccgcgag tcgatgcacc atgcgcagtt gactgagtcg
    15241 gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc aactggaagg gcttgggatg
    15301 acgcaacagc aggcatcagg ctggattgcc cagcagatca ccaatcaggg gctgattatt
    15361 tccgccaatg agatcttctg gatgtcagcc gggatattcc tcgtactgct ggggctggtg
    15421 tggtttgcta aaccgccatt tggcgcaggt ggcggtggcg gcggtgcgca ctaagtacaa
    15481 ctaagccagt tcatttgaac tggctttttt caattaattg tgaagatagt ttactgacta
    15541 gatgtgcagt tcctgcaact tctctttcgg cagtgccagt tcttcgttgc tgttgatgcg
    15601 tacgtcacgt tccagaatgc tacgcgcaat atcctgcgct tcctgcaacg agtgcatctg
    15661 gtaagtgcca cactggtaga cgttcagttc cgggatctga ttctgatcct gcactttcag
    15721 cacgtcttcc attgccgctt tccaggcatc agcaacacgc tgctcatctg gcgtaccaat
    15781 cagactcata taaaaaccgg tgcggcagcc cattggcgag atatcgataa tctctacacc
    15841 attaccgtta agatggttac gcataaaacc agcaaacagg tgctccaggg tatggatccc
    15901 tctttctggc atcacttctt tgttcggcac gcagaagcgc agatcgaaca cggtgattgc
    15961 gtcgccatgc ggggtgttca ttgttttcgc cacccgaact gcaggcgctt ccatccgggt
    16021 atgatcgact gtgaagctat ctaacaacgg catttagcca cctccggtaa tttttttaaa
    16081 aattttctga actctttctt cccaggcgag tctgagtata tgaaagacgc gcatttgtta
    16141 tcatcatccc tgaattcaga gatgaaattt tggccactca cgagtggcct ttttcttttc
    16201 tgtcaggtgt gtttttccag ccacaccgca aacggttcgg tatcagcggt ttccatttcc
    16261 tgctgacggc gttcagatgc ctcgcgctcg gctacaaaat cctcttcgcg cagaatttcc
    16321 agcggctctt cacgcagcag attacggtag gcttctgcaa acgctttgcc tgttccgcca
    16381 ataccagtat caatcataga ccttaagata cgggcagaga aagtcagatc gggattatcg
    16441 aagcaggcaa ccagttcatc acacactttc tgatacgcgt cgccgccgtt aatgctatcc
    16501 agcgtttgcg cgacgcgttt cagatcgcgg aacagatctt tacccacctg cagtaacggg
    16561 aactgcgcgg tttcgcagcc gatacccagc gtcaggcccg gtttgcgacc ttcaaggatc
    16621 acccggttcc agttaacgcg cgtacaggca agttcgctac tgctcatttc cggtgcatca
    16681 gccagcgcac accagaccat aaacaggtcg aggaatcgca cctgctgttc atctacacca
    16741 atcggcgaga acgggttgat gtccagcgaa cgcacttcaa tatattcaat gccgccacgt
    16801 aacagcgcat cagaaggtga ctcgccgctg cgggtaacgc gttttggacg aatcggcgca
    16861 tacagttcgt tttcaatctg cagcacgttg ctattgattt gcagcctctt accgtctttc
    16921 tcaataccaa tcttcgcgta ctcttccgat ggcgttttga ttgcctgttt aaggcccgct
    16981 acgtactcgt aaagatcgtt gaaggtaata ccaagattgc tttgcgattt attggtatag
    17041 ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt aatacatacc gcactcggtt
    17101 ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag aagaacaaat cgccggagat
    17161 gcaccaaaca gataaggaat gacccaaccg aaacgatagt aattgcggat aacgcggaaa
    17221 tagcccgcag aaattttctc tttggcatca gcgcccgaga tatcaccgca cttcgcttgc
    17281 cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg aaatggtttg catcagcgcg
    17341 ccgtagcgat ttttcagccc ttcacgatac agcgttttaa agcgtccggt gttagaagtg
    17401 ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt agcatggcat acttaacggc
    17461 cacatccgct catcgcccat attgcgcgcc gtataacgat gcagatcgcg cataaaggtc
    17521 agcatatgtt caatatcacc atccactggt gtaatgaatt ccagcaatgc ttccgcaaaa
    17581 tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg cttcaggatg acctgttgtt
    17641 gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct ccagcccacg ctgtatcccc
    17701 tttaacgcct gaggatgttt ttccagccag gccagcgcct gtgatacgtc cgggatcaaa
    17761 ttgacctccc gcctgtcaaa atcgttttaa ttagcataac tgtaatggtg accatatgtg
    17821 caggcctaca attagtgcca ccacatcatg ccctgaacgg tcgctgctgc aactgcaaca
    17881 tagcgtaacg ctttaccaag gcacaaaaaa aagattaccg gtccccacga gatgcgcatc
    17941 catcccgcta acagacacag taaatcgcca accacgggca tccagcttaa taatagcgtg
    18001 actgcaccat agcgtttcag ccagccgata gctttctctt gccagcgcga tgttttacgc
    18061 aatggaaaga aacgcccaag gataacgtta gttaaccctc caaggctatt acccattgtt
    18121 gctgttaaaa ctaaaaccca gggatgactg atcccggaaa gcaacattgc caccagcacg
    18181 acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac tactggcaaa caacgagaaa
    18241 agcgataacg cttcactcac agcaagcgaa cgtccacagc gtccatacct gcggcacggg
    18301 ccgcctgaat accgaaatcg gcatcttcaa agaccacaca ctgcgtcggt tgcacgccca
    18361 tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt atggtgtttg acgtgatcgg
    18421 cagcgacgac ggcgtcaaaa taatggcgta atcccaggtg cgccagcaat gcctcagcga
    18481 tggcgctttc actccccgtt cctacagcca ttgggcgacg accatgccaa cttttcacca
    18541 catcaacaag aggaagcggt tcgacgctat ccagcagcat acttcttact gcttctgttt
    18601 tttcacgcgc taacgcatgc gggtcgagat cggcctgatt cagctcaata attgcctgag
    18661 caatacgcca ggtgggcgat ccattaagcg caatcatcgc ctgaatatcg tactgaagac
    18721 cgtagtgccc taatacttcg cgccacgctt tacggtgcgt aggctccgta tccaggattg
    18781 tgccatccat atcaaaaatt aaacctgcat aacgctcgta catggtcttc tcgcaagtcg
    18841 aaaaatatga cgctacttta gcgtaattgc ttgattttgt cgctgatggc gaaggagaag
    18901 aaaattcagg aaatggataa aggagttatt tagaaatgag atatttttga aggaaatttt
    18961 ttggagaaga tggtgcatcc gggaggatga ctcactgacg ttcgcccttc gggccgttgc
    19021 tggcgcaacg ttatccttcc tggtgcctgc ggttaatacc gctatctctt cgataccttc
    19081 attgctgaag gttacggaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg
    19141 gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaaa cttattctac
    19201 tgctgatatt gataccgcta ctaaagccat atcaattaag agatggtgca tccgggagga
    19261 ttactcgcct tgcggctcgc ccttcgggcc gttgctaaag caacgttatc atccctggtg
    19321 cctgcggtta attccgctac ctcttcgata ccttcattgc tgaagatttc ggagaatatg
    19381 gtgcatccgg gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag
    19441 ctgagctacg gatgcatcgg gaaaatttat tttactgctg a
//
LOCUS       DAZPBM010000074        19356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPBM010000074 DAZPBM010000000
VERSION     DAZPBM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.074"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            219..1316
                     /gene="mltA"
                     /locus_tag="ACHUZQ_002344"
     CDS             219..1316
                     /gene="mltA"
                     /locus_tag="ACHUZQ_002344"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1026023.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1393..2199
                     /gene="tcdA"
                     /locus_tag="ACHUZQ_002345"
     CDS             1393..2199
                     /gene="tcdA"
                     /locus_tag="ACHUZQ_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1026024.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2250..2693)
                     /gene="csdE"
                     /locus_tag="ACHUZQ_002346"
     CDS             complement(2250..2693)
                     /gene="csdE"
                     /locus_tag="ACHUZQ_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1026025.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2693..3898)
                     /gene="csdA"
                     /locus_tag="ACHUZQ_002347"
     CDS             complement(2693..3898)
                     /gene="csdA"
                     /locus_tag="ACHUZQ_002347"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1026026.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4090..4317
                     /locus_tag="ACHUZQ_002348"
     CDS             4090..4317
                     /locus_tag="ACHUZQ_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1026027.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4668..5585
                     /gene="gcvA"
                     /locus_tag="ACHUZQ_002349"
     CDS             4668..5585
                     /gene="gcvA"
                     /locus_tag="ACHUZQ_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1026028.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5604..5999
                     /locus_tag="ACHUZQ_002350"
     CDS             5604..5999
                     /locus_tag="ACHUZQ_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1026029.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            5992..7092
                     /gene="rlmM"
                     /locus_tag="ACHUZQ_002351"
     CDS             5992..7092
                     /gene="rlmM"
                     /locus_tag="ACHUZQ_002351"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1026030.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7136..7867)
                     /gene="fucR"
                     /locus_tag="ACHUZQ_002352"
     CDS             complement(7136..7867)
                     /gene="fucR"
                     /locus_tag="ACHUZQ_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1026031.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7925..8347)
                     /gene="fucU"
                     /locus_tag="ACHUZQ_002353"
     CDS             complement(7925..8347)
                     /gene="fucU"
                     /locus_tag="ACHUZQ_002353"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1026032.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8349..9767)
                     /gene="fucK"
                     /locus_tag="ACHUZQ_002354"
     CDS             complement(8349..9767)
                     /gene="fucK"
                     /locus_tag="ACHUZQ_002354"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR1026033.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9876..11651)
                     /gene="fucI"
                     /locus_tag="ACHUZQ_002355"
     CDS             complement(9876..11651)
                     /gene="fucI"
                     /locus_tag="ACHUZQ_002355"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1026034.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11684..13000)
                     /gene="fucP"
                     /locus_tag="ACHUZQ_002356"
     CDS             complement(11684..13000)
                     /gene="fucP"
                     /locus_tag="ACHUZQ_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1026035.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13547..14194
                     /gene="fucA"
                     /locus_tag="ACHUZQ_002357"
     CDS             13547..14194
                     /gene="fucA"
                     /locus_tag="ACHUZQ_002357"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1026036.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14222..15370
                     /gene="fucO"
                     /locus_tag="ACHUZQ_002358"
     CDS             14222..15370
                     /gene="fucO"
                     /locus_tag="ACHUZQ_002358"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1026037.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15475..16230)
                     /gene="xni"
                     /locus_tag="ACHUZQ_002359"
     CDS             complement(15475..16230)
                     /gene="xni"
                     /locus_tag="ACHUZQ_002359"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1026038.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16342..17709)
                     /gene="sdaB"
                     /locus_tag="ACHUZQ_002360"
     CDS             complement(16342..17709)
                     /gene="sdaB"
                     /locus_tag="ACHUZQ_002360"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1026039.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17767..19056)
                     /gene="sdaC"
                     /locus_tag="ACHUZQ_002361"
     CDS             complement(17767..19056)
                     /gene="sdaC"
                     /locus_tag="ACHUZQ_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1026040.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4750 a         4778 c         5107 g         4721 t
ORIGIN      
        1 ccaccccgcc tccgtggatg cgcactatac tccgctcgcg ttttcatgca accttttttt
       61 taccccgatc acggatctgg catgattttg aacaaaaaca aatcactaaa gcctattttt
      121 tgtgcaaaat ttgcgtcagg gagaggctgc tcatttcatt aaacgcggcg gtttgttatc
      181 ttcgttgcgc cttatttttt aacctgaaga agagaacaat gaaaggacgt tgggtaaagt
      241 accttcttat gggcacggtt gtggcaatgc ttgccgcctg ctcttccaaa ccaaccgatc
      301 gcggacagca atataaagac gggaaattta cccagccttt ctctctggtg aaccagccag
      361 atgccgttgg cgcgccgatt aacgccggtg attttgccga gcaaattaac catatccgta
      421 attcgtcacc gcgtctgtat ggcaaccaga gtaatgttta taacgcggtg caagagtggc
      481 tgcgcgcagg cggtgatacc cgcaatatgc gccagttcgg cattgatgcc tggcagatgg
      541 aaggtgccga caactatggt aacgtgcagt ttacaggcta ttacacgccg gtaattcagg
      601 cgcgccatac ccgccagggc gagttccagt atcctattta ccgtatgccg ccaaaacgtg
      661 gtcgtctgcc gtctcgtgcg gagatctacg cgggggcatt gagtgataaa tatattctcg
      721 cttacagtaa ctccctgatg gataacttca ttatggatgt gcagggtagt gggtatatcg
      781 actttggtga tggcagtccg cttaactttt tcagctatgc agggaaaaac ggtcatgcct
      841 atcgcagcat tggtaaggtg ctgatcgacc gtggcgaagt gaaaaaagaa gatatgtcga
      901 tgcaggcgat tcgtcactgg ggcgaaacac acagtgaagc cgaggttcgc gagctgctgg
      961 aacagaaccc gtctttcgtc ttctttaaac cgcaatcttt tgcaccggtg aaaggggcaa
     1021 gtgcggtgcc gctggttggt cgcgcgtcag ttgcctctga tcgttccatt attccgccag
     1081 gtactacctt gctggcagaa gtgccgttgc tggataataa cggcaaattt aatggtcagt
     1141 acgaactgcg tctgatggtg gcgctggatg tcggtggtgc aatcaaaggc caacacttcg
     1201 atatctatca agggatcggg ccggaagccg gacaccgcgc aggttggtac aaccactatg
     1261 gacgtgtctg ggtgctgaaa accgccccgg gcgcaggtaa cgtctttagc ggctgatgtg
     1321 ttattctgta taccgttttt acggatacga cagggtgagg gataacctca ccctttttaa
     1381 ttctgaggtg ctatgtctgt ggtaattagt gatgcatggc gtcagcgttt tggtggcaca
     1441 gcgcgtctgt atggtgaaaa agcgttgcaa ctgtttgctg acgcgcatat ttgtgtggtt
     1501 ggtatcggtg gtgtcggttc ctgggcggcg gaagcgctgg cgcgcacggg gattggcgca
     1561 atcacgctta tcgatatgga tgatgtgtgc gtcaccaata ccaatcggca aattcacgct
     1621 ctgcgcgata acgtcgggct ggcaaaagcg gaagtcatgg cggagcgtat tcgccagatt
     1681 aacccggagt gccgtgtaac ggtggtggat gatttcgtga cgccggataa cgtagcgcag
     1741 tatatgagcg tgggttattc gtacgtgatt gatgccattg atagtgtacg gcccaaagcg
     1801 gcgctgattg cttattgtcg gcgcaataaa atcccgctgg tcacgaccgg tggcgcgggt
     1861 gggcagattg acccgacgca gattcaggtt accgatctgg cgaaaacgat tcaggatccg
     1921 ctggcggcga agttgcgcga gcgcctgaaa agcgattttg gcgtagtgaa aaacagtaaa
     1981 ggtaagctcg gcgtcgattg cgtgttttct actgaagcgc tggtgtaccc gcagtcagac
     2041 ggtactgtgt gtgcgatgaa agccacggca gaagggccga agcggatgga ttgtgcatct
     2101 ggatttggcg cggcaacgat ggtgaccgcc acctttgggt ttgttgcggt ttctcatgcg
     2161 ctgaagaaga tgatggcgaa agcggcgcgt cagggttaaa caatcaggtc ggatgagacg
     2221 cgaacgtctt atccgacccg gttctatgct taaacctgct tcgtcgcagc gataatcgcc
     2281 tcgcttaacg catttaaccc ctggctgcgt gaggcgctaa gttgcgcacg caatcccagc
     2341 tcatcaaaca atgccagcgg cgactgtgcc tgcaattcgg cggcggtttt cccctcaacg
     2401 gcagtcaaca gcaccgccag caggccgcgc acaatgcgcc cttcgctatc gccaaagaag
     2461 tgcattttac cgttttcagt cacggtatat cccagccaga cgcggttttc gcatccggca
     2521 atctctttag cctgcgcttt taactcgtct ggcaaagccg gaagctgttt ccccagcatg
     2581 atcaactggc gatatttatc ttcccattgc gacaacggtg cgaaggtatt gcgtaacgtt
     2641 tctgcggtta cggttgtgcc gaacggatgt ccggcgaatt gcgggttcgt cattaatcca
     2701 ccaataattc cagcgcgcgg tcaacggcat tcaccagcgc atccacatca ctctttgtat
     2761 tatatggcgc aaaagaggcg cgcagtgtgc cggttacgcc taattctgcc agtagcggct
     2821 gagcgcaatg ttgcccggcc cgcagggcaa taccgtactc cgccagcagc gtcaccatat
     2881 cgctgtgatg aacgccagca aaatcaaagg ccagcaggct ggaatcctgg cagcggaatg
     2941 agcggaagcc aggacgtttt gccagcgcat cttccgccag cgtcgccagg ctgcgactcc
     3001 aactttctgc ctggttgata tcgtaatctg ccagccattc cagcgccgcg cttaatccta
     3061 tgacaccagc cacatttggc gttcctgctt ccagtttcca cggcgcagat tgagtcgtga
     3121 agccgtcaaa actcacttcg tgaatcattt tgccgccacc cagccagggc gacatcgctt
     3181 ccagcagttc tgatttgcca tacagcacgc cgatacccgt cgggccatac agtttgtgac
     3241 cagagaaagc atagaaatcg atatctagtt gctgaacatc tgcggggaaa tgcaccgccc
     3301 cctgagcacc atcaaccatc accaccatcc cggctgaatg agcaaaggta atcgctcgcg
     3361 ccagatccgg gcaaccgcca gtaacgttcg acatctgacc caacgccaga atccgactac
     3421 ggggagtaat cagttctggc aacaaatcga catccggcag tcgctgcgca ttaagcggca
     3481 atttcaccac tttggctcca gtttgttggg cgaccatcag ccaggggacg aggttagcgt
     3541 ggtgttctgc cacgctgaca ataatctcat cgcccggttg cagacgcgga cgcgcatagc
     3601 attgtgccac catgttgatg gattcagtgg tgccgcgcgt ccagacgata gttttatcat
     3661 ccggtgcatt cagtaattgc gccactttct ctcgtgcagc ttcataacgc gcggtcaggc
     3721 gttgggcttc ggcaaactgg ctgcgatgga cgtttccggc gctcaggctg taaaactgtc
     3781 gagtggcttc aaccacggct tcaggtttaa gcgcggtcgc ggcgctatcg agatagacgc
     3841 ccgcatcctg tagtgcggga aactgggcgc gaaactgcgc gggattaaaa acgttcatgg
     3901 tactcctcgg cttgatgcac cgatcgtgac gcaaaattca ctttgataca aggagagtgc
     3961 taaccatcag aattatctgg atatcctggc gaagccgcgt aagtaacata tgctgaatag
     4021 tgttaggcgt taattttagc ctgttaatca atactattaa gatttattaa tcgctttaag
     4081 gagaagatga tgaaaaagac tgccgcaatt atttctgcct gtatgctgac ttttgccctg
     4141 agcgcctgtt ccggttcgaa ctatgtgatg cacaccaatg acggacgtac catcgtctct
     4201 gacggcaaac cacagactga taacgatacc ggtatgattt cgtataaaga cgctaatggc
     4261 aacaaacagc agatcaaccg tactgacgtg aaagagatgg tcgaactgga tcagtaattc
     4321 gcgatcgcaa ggtaaaaaaa agcaccgcaa ttaggcggtg ctacattaat cactatggac
     4381 agacagggta aatgtacagg aagtgaaaaa aggtagcttt gctaccatgg tctgaatcgc
     4441 agaccaattg caaacacaac aacacaacat cacaaccgta agccaaaagt tcaccagaac
     4501 acgcattccg ataaaacttt tcgttccggc tcaggaagtg ccgccactat aggtatttgc
     4561 tggtagaagc tcaacggaca atttataatg gctcagatta aaaaaactaa taggttacac
     4621 agtgtgatct aattgttaaa ttaatttaac atcaaagttt aaaagccatg tctaaacgat
     4681 taccaccgct aaatgcctta cgagtttttg atgccgcagc acgccattta agtttcactc
     4741 gcgcagcaga agagcttttc gtgacccagg ccgcagtaag tcatcaaatc aagtctcttg
     4801 aggatttttt ggggctaaaa ctgttccgcc gccgtaatcg ttcactcctg ctgaccgagg
     4861 aagggcaaag ctatttcctc gatatcaaag agatattttc gcaattaacc gaagcgacgc
     4921 gtaaactcca ggcccgtagc gccaaggggg cgttgacggt cagtttactc cccagtttcg
     4981 ccattcattg gttggttccg cgactttcca gctttaattc agcttatccg ggaattgacg
     5041 ttcgaatcca ggcggttgat cgtcaggaag ataagctggc ggatgatgtt gatgtggcga
     5101 tattttatgg tcggggcaac tggccggggc tacgggtgga aaaactgtac gccgaatatt
     5161 tattgccggt gtgttcgccg ctactgctga caggcgaaaa acccttgaag accccggaag
     5221 atctggctaa acatacgtta ttacatgatg cgtcacgccg tgactggcag acatataccc
     5281 gacagttggg gttaaatcat atcaacgttc agcaagggcc aatttttagt catagcgcca
     5341 tggtgctgca agcggctatt cacgggcagg gagtggcgct ggcaaataac gtgatggcgc
     5401 aatctgaaat cgaggccgga cgtcttgttt gcccgtttaa tgatgttctg gtcagtaaaa
     5461 acgcttttta tctggtttgt catgacagcc aggcagaact gggtaaaata gccgcctttc
     5521 gccaatggat cctggcgaaa gccgctgctg aacaagaaaa attccgcttt cgttatgaac
     5581 aataatttac gtagggtacg accatgacca gccgttttat gctgattttc gccgccatta
     5641 gcggcttcat ttttgtggct ctgggcgctt ttggcgcgca tgtgttaagt aaaaccatgg
     5701 gggccgttga gatgggctgg atccagaccg gcctcgaata ccaggcgttt catacgctgg
     5761 cgatcttagg tctggcggtg gcaatgcagc gtcgcatcag tatctggttt tactggagta
     5821 gcgttttcct cgcgttaggc acggtgttgt tcagcggcag cctttattgc ctggcgctgt
     5881 cccatctgcg tttgtgggca tttgtcacac cggttggcgg cgtgagcttc ctcgcgggct
     5941 gggcgttaat gttagttggt gctatccgtt taaagtgcaa gggcgtaagt catgaataag
     6001 gttgtattgc tgtgccgtcc gggctttgaa aaagagtgcg ccgcagaaat taccgataaa
     6061 gccggccagc gggaaatttt cggttttgcc cgcgtgaaag agaatgcggg ttatgtcatt
     6121 tatgaatgtt atcaacctga tgatggcgat aagttaatcc gtgagctgcc gttcagttca
     6181 ttaatttttg cccgccagtg gtttgtggtg ggggaactct tgcagcattt gccgccagaa
     6241 gatcgtatta cccccattgt cgacatgttg cagggcgtag tagagaaggg cggtgaactg
     6301 cgtgttgaag ttgccgatac caacgaaagc aaagagttgc tgaaattctg ccgtaaattc
     6361 accgtgccgc tacgcgctgc cttgcgcgat gccggggtgc tggcgaacta tgaaacgccg
     6421 aagcgtccgg ttgtgcatgt attctttatt gcaccaggct gctgctatac cggttactca
     6481 tacagcaaca ataattcgcc gttctatatg ggcattccgc gcctgaaatt tccggcagag
     6541 gcgccgagtc gttccacgct caaactggaa gaggcatttc atgtgtttat tcctgcggat
     6601 gagtgggatg aacgcctggc gaacgggatg tgggcggtgg atttaggcgc ttgccctggc
     6661 ggctggacct accaactggt gaagcgcaac atgtgggttt attccgtcga caacggcccg
     6721 atggcgcaaa gtctgatgga taccgggcag gtgacgtggc tgcgggaaga cggtttcaaa
     6781 ttccgtccga cacgcagcaa tatctcctgg atggtatgcg atatggttga aaaaccggct
     6841 aaagttgcgg cattgatggc gcagtggctg gttaatggct ggtgccgtga aaccattttc
     6901 aatctcaaac tgccgatgaa aaaacgctac gaagaagtat cacacaatct ggcgtatatt
     6961 caggcacagc ttgatgaaca tggcataaat gctcagattc aggcgcggca gttgtatcac
     7021 gatcgcgaag aagtgacggt gcacgtccgc cgcatctggg ctgcggtggg tggtcgtcgc
     7081 gacgagcgat aacaggcaag gccggggcgt cgtcttcggc ctgctaccct ttccgtcagg
     7141 ctgttaccaa agaagttgca acctggcgct catcagaaat aatgtgcgtt acctcatcca
     7201 gatgcccgat gcgggcttcc cctgaacgat taaatttact tttatcaatc aataacaacg
     7261 attgcgcggc acgttttaat agcatcgatt tgtaatcagc gttgatcgca ttggagtccc
     7321 acagtgcgcc gctgctatcg atcccttcac aagaaaaaat aaacagatcg atttccagcg
     7381 atttaagttg ggaaatcagc gagggattga cgtaacagcc atatttgcgc tcaagtgtgc
     7441 cgccggaact gatcagttga atgcgttcgc gtttaccgag ttcatggcaa atcggatggc
     7501 tattggtgaa gacctgaatg ttgatgtcag gcaactggcg tgccagatac cagcaagttg
     7561 aactggcgtc taaggctatc accatccctt cttcaatcca cgcgagcgcc tcgcgcgcga
     7621 tatctgcttt atgcgcataa tggcttttca gcctgatgtg aaagggatcg ccactgtctt
     7681 gattttgacg gtggatatat ttagcgcgtc catgattgcg cagaatttta ccctgcgtct
     7741 gtaattcatt gagatcgcga cgaatggttt ctttactgac ctttagctgt tcagagagag
     7801 cttccgtggt caggctggta tggttcagca gcaggtcgac tatcgcttgc tggcgtgccg
     7861 ctttcatatt tttcacccca tcattcgata accgcccgcg tcatgcgggc gtaccgatat
     7921 gagattacgg tgttacccct ttttttaaaa gaatattccc gtacttcgct cgttcgcctg
     7981 tgataacgat cgcaaaggct ttttgcgccc gttcataaaa cgcaaaacga ttgatgcgga
     8041 tgatgtcagg acacggggct tgtagtgaaa gcgcattacg gtaacgtcgt tctacttcag
     8101 gatcgagagt gtcaccttct accgctgcca tcatcaccag cggcggggca taactgtcca
     8161 gctcaaataa tgggataatc gcttggagca agtcgctcac caacaggcca tcagcgcgga
     8221 tcacctgcgg tcccatcgaa tgggcgggaa agtgagcatc ggaaaaaata atttcatctc
     8281 catgtcccat ctctgccagc actttcaata gttcgggaga aattaacggc gaaattgttt
     8341 tcagcatttc acacttcctc tataaattca ggttcagttt gcgggtagaa ataacggtac
     8401 tgataatgaa tctgtgcacg ggcttcttcc gggctgttaa attcccctac gccataccaa
     8461 ccgaacagcg cagctcctgc gacggtcgtt tcggcatcgt cgagaacttt taccggaata
     8521 tcaagcatat tggctttaat ctgattccac aatgtgttgc gacttcctcc accgactaac
     8581 aataattcag aggctttaaa gtgcccgatt ttttccagca tctgtagatt gcgctgtaat
     8641 tgcgcagtta acccttccag cgccgcgcga tagaaatgcc cccgcgtggt attaagcgtc
     8701 actccttgcc agccagcgtt ctgacacgac aataaatcac actgcatttt cacaccatcc
     8761 gcgccaggtg cgatcagacg agcttcttca atcaacattt gccagggtgt ttcagccgtc
     8821 cagaacagtt ttctcaccca ttccagcacg ccggatgcca gccattgcat acctgggtta
     8881 tacaaccctg cctggctatc cagttcgcag gtagaatcgg cgtactgact taacagcgaa
     8941 gtatcaacct gggcgctacg gaccattaaa atttcccatg taccggaaga gagcacgggt
     9001 tcattttggt cggcaccagc gccaaaaagg gcgaactggg tatcgtgacc tgcggaaatc
     9061 accggtatgc caacgggtaa gccgagcatt gctgcggcgc tgttctgtag cgtaccaatc
     9121 tgttcacccg cttccaccag acgagggaag agtcggcgtg gaataccggt ggcttgtaaa
     9181 atttgcggac tgaaatcgcg ttgctggata tccagcatct ggctggttcc ggccatcgtg
     9241 atatcagtag tgaattcgcc ggttaaacgg tggttaatca gcgacgaaat aaagagccag
     9301 gcgtgcgcgc gttccagcag ttgtggatga ttttctttca accacaccaa cttatataac
     9361 gtattgaaac taaaggctcc gacgccagaa atagcctgca accgctgtgc ggagatcaac
     9421 tgttcaatat tgtccataac cgctgctgtt cgcggacatt tccagctaat gatcggatag
     9481 agcagattgc cttgcttatc taccagagcg ccatccacac caaaggtggt gacggcgata
     9541 ccgcggatgt ggcattcagt cagttcacta ttgatttgcc gacagcaatc agcaaagcgt
     9601 tgcaaaatgg cgtctaaaga ccactggtgc caggtgttgt tttccatcgc gatatcgctg
     9661 gcattaggcg ttgaggcgcg ggcaacaatt tttccctgcc gattaaccgc gatggccctg
     9721 acattggtcg cgccacagtc gagtaccagg ataacttctt gtttcataat cgctcctgca
     9781 atatagccgg ataacattgc ttatccggct aaccactctt gcttgttcag gcccggtagc
     9841 cggagcgacc gggcatcaca tcagggagta atgtattaac gcttgtacaa cggaccgtag
     9901 ttctggcaag cgcggtaatc ctggccttca atatccatgc cgtgcgcagc ccaggcagaa
     9961 ggacgataca ctttggtctc ttcaacgttg tgcatacata ccgggatacg cagcatggag
    10021 gcgagagtga taaagtctgc gccaacgtgg ccgatggtca gaaccccatg gttagcgccc
    10081 cagttcgcca ttaccgagta cacatccgta aacggccctt taccggtgag gcgcggtgca
    10141 aaccaggtgg ttggccaggt tgagttggtg cgtttgttga ggatgtcatg cacatccttc
    10201 ggcaattcca cgctccagcc ttccgcgatt tgcagtaccg atcccaggcc tttgatgatg
    10261 ttgacacgag tcatggtgaa cgggacgccg ccttcggtaa ggaagcggga agagtaaccg
    10321 ccgccacgga agtattcgtg gatcgccggg caccattcgg tagcggcgag gcaagcgtca
    10381 gcctcttgct gagagatttc ccagtgtggc ttcatcgtcg ggttaccttc gctgtcgcgt
    10441 tgtttacagg aaccgtccag cgcagcagaa cctgagttga tcaaatggat gatgccgtgt
    10501 tctgccagtc catcgagttt atgccccgtt acacgctcaa ttgcttctgg tgaccagtag
    10561 gtacgcacat cggcaaatac ctgagcggtg ccggtgagct ggtgacccat tagcattgcc
    10621 acgccgttaa gactgtcgtt ttcggtcgcc acgacaaagg gttcgcgcac gccattccag
    10681 tcaaatgaac tgttgaggat cgcttcggcg gtgtcaccat tgggatattg atcggtccag
    10741 tgacgttgcc cctggaagcc cgcagcgatg gcgttgtagc caagtgattc ttccacgcga
    10801 ccaatatcgg ccagtttgct gttgccttgc atcatgtcgc ggatacacat cgccatcagt
    10861 aaactttcgc gcagaactgc gcggctttgc tcggcattgc gttgatactg tttgttattt
    10921 tcatcttcgc catagcggaa gtttttatca gcccaggtca gtgccatttc cagttcggct
    10981 tcgtcgtaaa tcttctgatc gatacggcga cgcagttcgg tcatatccac cgcctggact
    11041 ttcattccca gccaggattc aaagaagttg tgatcaacaa tagaaccggc gatacccatc
    11101 gaaacgccgc ccagcgacag atagctttta cctttcatgc tggcgacggc caaaccggcg
    11161 cgggcaaagc gcagcagttt ttcttcaaca tcggcaggaa tcgatgtgtc atcggcatcc
    11221 tgaacgtcat gaccgtaaat ggagaatgcc gggatgcctt tctggctgtg agctgccaga
    11281 gccgctgcca ggtaaacagc gccgggacgt tcagtgccgt taaagcccca aatggctttc
    11341 gggcgggttg gatccatgtc gatggtttca ctgccatagc accagcaagg cgttaccgta
    11401 atggtgaggc ctacattctg gctgctgaat ttttcttcgc aagcagcggc ttcagccata
    11461 cccgcgatac aggtatcgga aatgacacac tcgacggcag ctccgcaggc atggcgcagt
    11521 ttctcggtca gcagtgcggc cgtagctttc gccatattca ttgtttgttc ttcaagcgac
    11581 tcacgaacac ccatgcgacg accgtcaata accgggcgga taccaatttt cggtaagctg
    11641 atttttttca ttacagattc ctcactttat tcggaaaata tgttcagtta gttgccgttt
    11701 gagagcggaa acgggcaaag ataaagatga ccgcgaagca gagtgtgggg atcagttcag
    11761 cagtggggat gttgcccgcc gcgtcactga caaaacccat gaccggagtg acaataccgc
    11821 cgccaataat ggtcataacg atgaaggacg aaccgtattt ggtgtcctgg ccgagattct
    11881 taatgcccag cgagaagatt gttgggtact gaatcgacat aaaggcgctg cataaagtca
    11941 gggctattaa gcccacatga ccgccagcga aggctgagat caggcacagt gccatagcga
    12001 ttaatgcgta ggcggccagg actttgtgtg gtgcgaagcg actgatgagc caggtaccgg
    12061 tgaaacgacc aataaagaag cacaccatgg ttccggttaa atagttagcg gcaaagcctg
    12121 cagtcatacc tagaatttct tctacagcgt agcgaatcaa atagctccag caggccgttt
    12181 gtgcgccaac gtagcagaat tgcgctaata ctgcccagcg ccagtggcga atacgcgcta
    12241 ggcgagaaag cgatgcggag aacgatcctt gtttggcgtc actgtgatta tcactctgca
    12301 atgccgggaa tttcgtcagc atgatcagca gggcgaccag taacacgata gccacgatga
    12361 tcatataagg tgtctgtacc gataatacca ggctgtgttt atacgcactc aattgctctg
    12421 gagacatttt atcgagaacg tcttgcgatt gatgtggcac gttagacaaa ataagacttt
    12481 gcccaaagac aaccgcgata attgcgccaa acgagttaaa tgtttgcgca agatttaagc
    12541 ggaagtgacc actgctttcc ggccctaata ccgtaacaaa agggtttgcg gcagtttcca
    12601 gacaacctaa tccggctgca ataataaata ggccaactaa aaataaggtg tagttcatta
    12661 tttctgcagc gggccagaat aatgcagcgc ccagggcata taaaaataac ccagtaataa
    12721 ttcctgcttt ataactgagt tttttcatca atatcccagc aggaattggg ataatgaaat
    12781 aaccaaagta aaaggccgat tggatcaggc cagcctggaa atttgtcagc gtaaaagcct
    12841 gctggaattg aggtaataaa atgtcgttaa ggttattggc taccgcccaa agaaaaaaca
    12901 gtgagcacag cagcgcgaat ggaataatgt aacttctgct ttgccctgca tctttatcta
    12961 ccgcacggta actctgcgtt tgtattgatg tgtttcccat agcatcctct taggttcaga
    13021 agcttaagca cgtaatcggt tatgttttca aaagtgcaaa accagcgagt tcgggcttca
    13081 tatcagtgaa tatttgcctg tctgaaatga actcatggat tcattttcaa ataaaaaacg
    13141 ctctgtaata tgacggcggt cacacttatt agctggaagg tgactttatg tgactaccat
    13201 cactttaata atcgaaattt catgcccgtt tcagaaaata aatcataaaa aacacaaacc
    13261 agatgactgt attaattgtt tatgccgaaa acgggcattc ctggcttggg tgaatagaaa
    13321 aatgaccgtt ttgataaata tttttaagag cggtcatttt taatggggtt ataaaaagag
    13381 aacttgcagt tatttactgt gattagttga gctaggtcac aaaatataag cttgtgaatt
    13441 tctttcttgt taatcaggaa gaagatggtg ggtaattaaa cggctaattc aatagtgtga
    13501 aaggaacaac attattgccc tgttttgaat cagagagagg tagctaatgg aacgaaataa
    13561 acttgctcgt cagattattg acacttgcct ggaaatgacc cgcctgggac tgaaccaggg
    13621 gacagcgggg aacgtcagtg tacgttatca ggatgggatg ctgattacgc ctacaggcat
    13681 tccatatgaa aaactgacgg agtcgcatat tgtctttatt gatggcaacg gtaaacatga
    13741 ggaaggaaag ctcccctcaa gcgaatggcg tttccatatg gcggcctatc aaagcagacc
    13801 ggatgccaac gcggttgttc acaatcatgc cgttcattgc acagcagttt ccattcttaa
    13861 ccgaccgatc cccgctattc actacatgat tgcggcggca ggtggtaatt ctattccttg
    13921 cgcgccttat gcgacctttg gaacacgcga actttctgaa catgttgcgc tggctctcaa
    13981 aaatcgtaag gcaactttgt tacaacatca tgggcttatc gcttgtgagg tgaatctgga
    14041 aaaagcgtta tggctggcgc atgaagttga agtgctggcg caactttacg tgacgaccct
    14101 ggcgattacg gacccggtgc cagtgctgag cgatgaagag attgccgtag tgctggagaa
    14161 attcaaaacc tatgggttac ggattgaaga gtaatttcgt aaagcaacaa ggagaaggat
    14221 gatggctaac agaatgattc tgaacgaaac ggcatggttt ggtcggggtg ctgttggggc
    14281 tttaaccgat gaggtgaaac gccgtggtta tcagaaggcg ctgatcgtca ccgataaaac
    14341 gctggtgcaa tgcggcgtgg tggcgaaagt gaccgataag atggatgctg cagggctggc
    14401 atgggcgatt tacgacggcg tagtgcccaa cccaacaatt actgtcgtca aagaagggct
    14461 cggtgtattc cagaatagcg gcgcggatta cctgatcgct attggtggtg gttctccaca
    14521 ggatacttgt aaagcgattg gcattatcag caacaacccg gagtttgccg atgtgcgtag
    14581 cctggaaggg ctttccccga ccaataaacc cagtgtaccg attctggcaa ttcctaccac
    14641 agcaggtact gcggcagaag tgaccattaa ctacgtgatc actgacgaag agaaacggcg
    14701 caagtttgtt tgcgttgatc cgcatgatat cccgcaggtg gcgtttattg acgctgacat
    14761 gatggatggt atgcctccag cgctgaaagc tgcgacgggt gtcgatgcgc tcactcatgc
    14821 tattgagggg tatattaccc gtggcgcgtg ggcgctaacc gatgcactgc acattaaagc
    14881 gattgaaatc attgctgggg cgctgcgagg atcggttgct ggtgataagg atgccggaga
    14941 agaaatggcg ctcgggcagt atgttgcggg tatgggcttc tcgaatgttg ggttagggtt
    15001 ggtgcatggt atggcgcatc cactgggcgc gttttacaac actccacacg gtgttgcgaa
    15061 cgccatcctg ttaccgcatg tcatgcgcta taacgctgac tttaccggtg agaagtaccg
    15121 cgatatcgcg cgcgttatgg gcgtgaaagt ggaaggtatg agcctggaag aggcgcgtaa
    15181 tgccgctgtt gaagcggtgt ttgctctcaa ccgtgatgtc ggtattccgc cacatttgcg
    15241 tgatgttggt gtacgcaagg aagacattcc ggcactggcg caggcggcac tggatgatgt
    15301 ttgtaccggt ggcaacccgc gtgaagcaac gcttgaggat attgtagagc tttaccatac
    15361 cgcctggtaa atgcgctgat gtgataatgc cggatacgac gctagccgcg tcttatccgg
    15421 tatataagtg cattaaattc aatgtgttga taatcgtttg catccggctc gccgttaccg
    15481 taccaaccgc aattgctgta aattgccgtc gatatgcaaa tcggtttgta agcgggcaat
    15541 atcgcggcac agaaacgcca tctctttatg ggtttctaat tttttgcgcc acttttcggc
    15601 aaccgcatcc agattctcat atatcccttc cagactctga aactcgacca gcagctgcgt
    15661 ggcgcttttt ggtccgattc ccgcaacacc cggtaccttt gaactgctga tccccgcaag
    15721 tccccagtaa tcgggcaact gctgcggttg aacgccaaat tctttatcga taaatggcgc
    15781 atccagccaa cgtttctgga agtaatcacg aatacgtaat gtcggtgaaa gtaactgaca
    15841 gtagccttta tctgtcgaaa caatcgttgc ctgatgcccg gcctgtgtca ctttgaccgc
    15901 cagcgtggcg gctaagtcat cggcttcgtt gccgctggtt gaccagcacg ggacgccgcg
    15961 ttgctcaaag gcggcgcgta atgcaggcat ctcgtcgtgc aactcttccg gcattggcgg
    16021 tcgacccgct ttgtaatctg gtaaacgctg atgacgccag ccgctactgc ggttttcatc
    16081 atcaaaaacg gcgaccgcgt gggttggctg gctgtgcata atgagctgat cgagcgcatg
    16141 ctggcaggtt tcgacacagg gcgatccctg aacggcatga atgcgacgaa taagattcag
    16201 tgcatcgaca ataagcaaat gaacagccac gataaccccc ttaaagatca ggcgataagg
    16261 gtaacattcg atgccggaac aggctacggc tggatgagaa atcgggaaga ggcctcgcaa
    16321 aacgaggcct ctggagagcg attaatcgca ggcaacgatc ttcattgcca ggccgccgcg
    16381 agaggtttcg cggtacttgg cgttcatatc tttacctgtt tcgtacatgg tttcgataac
    16441 tttatcgagg cagacgcgcg gctcgctggt acggcgtagc gccatacgtg cggcgttcac
    16501 cgcttttact gccgcaatgg cgttacgctc gatgcatggc acctggacct gtccggcgac
    16561 cgggtcacac gtcagaccga ggttgtgctc catggcgatt tccgccgcga tgcacacctg
    16621 cgccgggctt gcacctaaca gttctgccag accagccgcc gccattgagc acgccacacc
    16681 aacttcaccc tggcagccga cttcagcacc agaaattgac gcgttcatct tataaagaga
    16741 accaatggca ctggctacca gcaggtaacg agccagtgag ttagcgttca cttcgcggat
    16801 gaacttgtcg tagtacgcca gcactgccgg gataatcccg cacgcaccgt tagtcggcgc
    16861 agtcaccacg cgtccgccag cagcgttctc ttcgttcact gccagtgcaa acatgttgat
    16921 ccagtcaaca accgccatcg ggtcagtggt ggttttatcc tggctgacca gcatccggcg
    16981 tagtgccgca gcacggcgtg gaacgcgcag tttgccaggc aacacgcctt cggtggaaat
    17041 accgcgctca ataccgccac gcatgacttc ccatacgttc gccaggtgct gttccagctc
    17101 ttctttgctg tgcagcgcca gctcattttt catcatcagg ccagagagtg acagcccggt
    17161 ttcctgacaa tgtttttgca gatcggctgc tgaactgtac ggataaggaa cttcaaccgg
    17221 tgcgctattc tgctggccaa aatgctcttc atcaacgata aagccaccgc caatagagta
    17281 gtaagtctgg ctgtaaacga ctttatcgcc cgccagcgcg gtaatgcgca taccgttttc
    17341 atgcagagaa aggttgtctg catggaagtt catgcactga tcaaccggga actccacttc
    17401 atgctgaccg tttgccagca tcaggcgacc atgagtattc acatcctgaa taaaaccagg
    17461 gatggaatcg atatccacgg tatccggcag gttacccgcc aggcccataa taatggcgat
    17521 atcagtgtgg tggcctttac cggtcagaga gagcgagccg tacacgtcaa ccaccacgcg
    17581 ggtcacgtct ttaagcagat tacgggcaat cagatcgtcg gtaaattgtt tacccgcttt
    17641 cattggtcca acggtatgag aactggaagg gccaatgccg attttgaaaa tatcgaatac
    17701 gctaatcata ggaaatacat cgcgttaaaa cggaggaagc gccgcccgaa agcggcgcga
    17761 aaggacttag ctgaacagag agtagaagat tgcggagatt gcaatcagac ccatcacgac
    17821 aacgaatacg ttgctgatgt gaccgctgta cttacgcatt gccggtactt tctgaattgc
    17881 gtacatcggc atcaggaaca ggatcatcgc gatgattggg ccgcccaggg tttcaatcat
    17941 acccaggatg ctcgggttca gggtggcaac aatccaggtc gttaccagca tgaacagcgc
    18001 agtgatacgg ttcagcttgt tgatttcgat agacttacct ttaccacgca gagatttaat
    18061 caccataccg ttgaagcctt cacgtgcgcc caggtagtgg ccgaggaagg atttggtgat
    18121 agcgataatc gcgataatcg gagccatcca cgcgataacc ggtgcgttaa agtggttagc
    18181 caggtaagac agaatcgaga tgttctgctc tttagccgca gccaggtctg ccggagtcag
    18241 gctcagtaca cagctgaaga cgaagaacat tacggtcagc accatcatga tgtgtgcgaa
    18301 tgccaggatc ttcgagcatt tctgttctgc catatcgccg tactcttcac gcttcgcaac
    18361 ggcgaaagaa gagatgatcg gagagtggtt gaaagagaac accataaccg gaattgccag
    18421 ccacagggtc atccacagac cgtttccggt tgcagatgca gtgtccagag acagcgtttc
    18481 cagtgctgcg ccgttccact gcgggatcag gtacagagcc agcagcatca gtacgccaac
    18541 aaacgggaat accagaatac tcattgcttt aacgatcatc tgctcaccga agcgaacgat
    18601 ggtcatcata cccacgatca ggatcagcga cagaatcgca cgcggcggtg gtgtcatacc
    18661 cagctggtga gacatgaagc tttcaactgt attggtgatt gccacgctat aaaccagcag
    18721 gatcgggtag atagcgaaga agtagagcag ggtaatcagt ttacctgcgc caataccaaa
    18781 gtgttcttct acaacctcgg tgatgtcttc gcctgggttt ttaccagaca gtacgaagcg
    18841 agtcaggccg cggtgagcaa aaaatgtcat cgggaacgca aggatagcca tgatgatcag
    18901 cgggatcata ccgccaacac cggcgttgat tggcaggaac agcacgcccg cgccgattgc
    18961 cgtgccgtaa aggcccagca tccacatggt gtctgtcttg cgccaggcac tacgagagtc
    19021 tttcgacgca atcgtgctgg tttgagtcgt ttccatctat ttctcctgga ggaatgcaaa
    19081 taattcagct tttagccagt tcagctcggg aaggtgcctg accgtaaaaa tgaaatctgt
    19141 ctatcggcga tggcttaatc atttaccgcc gtaataattt gagggcggaa agatacacac
    19201 aagttatgta tctatcagtg atcttgatcc caaatacaat aatctacttg gaagatagat
    19261 tatcccaacg ctttactctg taaatcaaaa gtaaaagaga atttaagggc aatatttgcc
    19321 cattaaacga catttgactt cttccggggc gcgcaa
//
LOCUS       DAZPBM010000075        19096 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPBM010000075 DAZPBM010000000
VERSION     DAZPBM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.075"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(179..1828)
                     /gene="pgi"
                     /locus_tag="ACHUZQ_002362"
     CDS             complement(179..1828)
                     /gene="pgi"
                     /locus_tag="ACHUZQ_002362"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1026041.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2065..2261
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2353..3702
                     /gene="lysC"
                     /locus_tag="ACHUZQ_002363"
     CDS             2353..3702
                     /gene="lysC"
                     /locus_tag="ACHUZQ_002363"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1026042.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3954..4226
                     /locus_tag="ACHUZQ_002364"
     CDS             3954..4226
                     /locus_tag="ACHUZQ_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1026043.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4359..5231)
                     /gene="rluF"
                     /locus_tag="ACHUZQ_002365"
     CDS             complement(4359..5231)
                     /gene="rluF"
                     /locus_tag="ACHUZQ_002365"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1026044.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5579..6416
                     /locus_tag="ACHUZQ_002366"
                     /pseudo
     CDS             5579..6416
                     /locus_tag="ACHUZQ_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6486..7292
                     /locus_tag="ACHUZQ_002367"
     CDS             6486..7292
                     /locus_tag="ACHUZQ_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1026045.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7302..7709
                     /locus_tag="ACHUZQ_002368"
     CDS             7302..7709
                     /locus_tag="ACHUZQ_002368"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1026046.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7709..8203
                     /locus_tag="ACHUZQ_002369"
                     /pseudo
     CDS             7709..8203
                     /locus_tag="ACHUZQ_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8269..9066
                     /locus_tag="ACHUZQ_002370"
     CDS             8269..9066
                     /locus_tag="ACHUZQ_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1026047.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9077..9901
                     /locus_tag="ACHUZQ_002371"
     CDS             9077..9901
                     /locus_tag="ACHUZQ_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR1026048.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9953..11181
                     /gene="sorE"
                     /locus_tag="ACHUZQ_002372"
                     /pseudo
     CDS             9953..11181
                     /gene="sorE"
                     /locus_tag="ACHUZQ_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11564..12253
                     /gene="pepE"
                     /locus_tag="ACHUZQ_002373"
     CDS             11564..12253
                     /gene="pepE"
                     /locus_tag="ACHUZQ_002373"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1026049.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12344..13975)
                     /locus_tag="ACHUZQ_002374"
     CDS             complement(12344..13975)
                     /locus_tag="ACHUZQ_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1026050.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14195..17878)
                     /gene="metH"
                     /locus_tag="ACHUZQ_002375"
     CDS             complement(14195..17878)
                     /gene="metH"
                     /locus_tag="ACHUZQ_002375"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1026051.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18078..18902
                     /gene="iclR"
                     /locus_tag="ACHUZQ_002376"
     CDS             18078..18902
                     /gene="iclR"
                     /locus_tag="ACHUZQ_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1026052.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         4497 a         5002 c         5006 g         4591 t
ORIGIN      
        1 tcacagacaa ggggatttat ctgataaaac aatgccgact tattgtcggc attgttttta
       61 aggttattta actcctcata ctatgcatta atgtcgatat tggcatcagg ctccggttgc
      121 cggatgcggc gtaaacgcct tatccggcct acatatcggc atcaggcacg catgatgatt
      181 aaccgcgcca cgctttatag cggttaatca gaccattggt cgagctatcg tggctgctga
      241 tttctttgtc atctttcagc tctggcagaa tacggttcgc cagttgttta cccaactcca
      301 cgccccactg gtcgaaggta aagatgttca ggatcacgcc ctgagtaaag attttgtgct
      361 catacagcgc aatcaacgca cccaggctga acggagtgat ttcgcgcagc aggatggagt
      421 tggtcgggcg gttaccttcg aacactttga acggcaccac gtagtcaagc gttgccggat
      481 ctttaccctg atcgcgatat tcctgctcaa ccacttcgcg ggatttacca aacgccagcg
      541 cttcggtctg ggcgaagaag ttagacagca gtttctggtg gtgatcagag agcgggttat
      601 gggtgatagc cggggcgatg aaatcgcacg gtaccatttt ggttccctgg tggatcagct
      661 ggtagaacgc gtgctgaccg ttagtgcctg gttcacccca gataatcggg ccagtctggt
      721 aatccacaac gttaccgtta cggtcaacat acttaccgtt ggactccata ttgccctgct
      781 ggaagtacgc cgcgaaacgg tgcatatact ggtcatacgg cagaatcgct tcagtttccg
      841 caccaaagaa attgttgtac cagatgccaa tcagcgccag caggacaggc aggtttttct
      901 cggcaggcgt ggtggagaaa tgcttgtcca tcgcgtgtgc gccagaaagc agttcaacga
      961 agttatcaaa gccgatggag agaacaatcg acaggccaat cgctgaccac aaagagtaac
     1021 ggccgccgac ccagtcccag aactcgaaca tgttggcagt atcaatacca aactcgccaa
     1081 cggctttggc attggtggaa agcgccgcaa agtgttttgc aacgtgtttt tcatcacctg
     1141 ccgctttcag gaaccagtca cgcgcgctat gggcgttggt catagtttcc tgagtggtga
     1201 aggttttaga tgctaccagg aacagcgtgg tttccgggtt tacttttttc agcacttcag
     1261 cgatgtgagt cccatcgacg ttagaaacaa agtgcatgtt caggtgattt ttgtacggac
     1321 gcagtgcttc ggtcaccatg tacgggccga ggtcagaacc accgatcccg atgttcacta
     1381 cgtcagtgat tgctttgccg gtataaccct tccactcacc ggagataatc gcttccgaga
     1441 aggttttcat cttctccagc accgcgttga cttccggcat tacgtctttg ccatcaacca
     1501 gaatcggggt attgctacgg ttacgcagcg ctacgtgcag cacggcgcgg ttttcagtgc
     1561 ggttgatctt ctcgccagag aacatcgact taatcgcgcc cgccagatcg cactctttcg
     1621 ccagatcctg taatttcgcc agcgtctctt cagtgatgcg gtttttggag taatccacca
     1681 gcatctgatc gtcgaatgtt gcggagaact tagaaaaacg atcgccgtct ttagcaaaaa
     1741 gatcggcgat cgtaacgtct ttcatttcat cgaagtgttt ctgtagtgcc tgccaggcag
     1801 cggtctgcgt tggattgatg tttttcatta gcaatactct tctgattttg agaattgtga
     1861 ctttggaaga ttgtagcgcc agtcacagaa aaatgtgatg gttttagtgc cgttagcgta
     1921 atgttgagtg taaaccctta gcgcagtgaa gcatttatta gctgaactac tgaccgccag
     1981 gagtggatga aaaatccgca tgaccccgtc gttgacaacc gccccgctca ccctttattt
     2041 ataaatgtac tacctgcgct agcgcaggcc agaagaggcg cgttgcccaa gtaacggtgt
     2101 tggaggagcc agtcctgtga taacacctga gggggtgcat cgccgaggtg attgaacggc
     2161 tggccacgtt catcatcggc tacaggggct gaatcccctg ggttgtcacc agaagcgttc
     2221 gcagtcgggc gtttcgcaag tggtggagca cttctgggtg aaaatagtag cgaagtatcg
     2281 ctctgcgccc acccgtcttc cgctcttccc ttgtgccaag gctgaaaatg gatcccctga
     2341 cacgaggtag ttatgtctga aattgttgtc tccaaatttg gcggtaccag cgtagctgat
     2401 tttgacgcca tgaaccgcag cgctgatatt gtgctttctg atgccaacgt gcgtttagta
     2461 gtcctctcgg cttctgctgg tatcactaat ctgctggtcg ctttagcgga agggctggaa
     2521 cctggcgagc gattcgaaaa actcgacgct attcgcaaca tccagtttgc cattctggaa
     2581 cgtctgcgtt acccgaacgt tatccgtgaa gagattgaac gtctgctgga gaacattact
     2641 gttctggcag aagcggcggc gctggcaacg tctccggcgc tgacagatga gctggtcagc
     2701 cacggcgagc tgatgtcgac cctgctgttt gtcgaaatcc tgcgcgaacg cgatgttcag
     2761 gcacagtggt ttgatgtacg taaagtgatg cgtaccaacg accgatttgg tcgtgcagag
     2821 ccagatgtag ccgcgctggc ggaactggcc gcgctgcagc tgctcccacg cctcaatgaa
     2881 ggcttagtga tcacccaggg atttatcggt agcgaaaata aaggtcgtac aacgacgctt
     2941 ggccgtggag gcagcgatta tacggcagcc ttgctggcgg aggctttaca cgcatctcgt
     3001 gttgatatct ggaccgacgt cccgggcatc tacaccaccg atccacgcgt agtttccgca
     3061 gcaaaacgca ttgatgaaat cgcgtttgcc gaagcggcag agatggcaac ttttggtgca
     3121 aaagtactgc atccggcaac gttgctaccc gcagtacgca gcgatatccc ggtctttgtc
     3181 ggctccagca aagacccacg cgcaggtggt acgctggtgt gcaataaaac tgaaaatccg
     3241 ccgctgttcc gcgcgctggc gcttcgtcgc aatcagactc tgctcacttt gcacagcctg
     3301 aatatgctgc attctcgcgg tttcctcgcg gaagttttcg gcatcctcgc gcggcataat
     3361 atttcggtag acttaatcac cacgtcagaa gtgagcgtgg cattaaccct tgataccacc
     3421 ggttcaacct ccactggcga tacgttgctg acgcaatctc tgctgatgga gctttccgca
     3481 ctgtgccggg tggaagtaga agaaggtctg gcgctggtcg cgttgattgg caatgacctg
     3541 tcaaaagcct gcggcgttgg caaagaggta ttcggcgtac tggaaccgtt caacattcgc
     3601 atgatttgtt acggcgcatc cagccataac ctgtgcttcc tggtgcccgg cgaagatgcc
     3661 gagcaggtgg tgcaaaaact gcatagtaat ttgtttgagt aaatactgta tggcctggaa
     3721 gctatatttc gggccgtatt gattttcttg tcactatgct tatcaataaa cgagcctgta
     3781 ctctgttaac caacgtcttt atcgagaata attgccttta atttttttat ctgcatctct
     3841 aattaattat cgaaagagat aaatagttaa gagaaggcaa aatgaatatt atcagttctg
     3901 ctcgcaaacg aattcccgta cactgaactc tctggctcac tgagggcacc atcatggcac
     3961 tcccccgtat tacccaaaaa gagatgactg aacgcgagca gcgtgaactt aaaacactgc
     4021 tggatcgggc gcggattgcg catggtcgcg tactgaccaa ctccgaaacc aacagcatta
     4081 agaaagagta catcgataaa ttaatggtcg agcgtgaggc tgaagcgaaa aaagcccgcc
     4141 aattgaagaa aaagcaggct tataaaccgg atcctgaagc gtcatttagc tggtcagcaa
     4201 atacgtcgac gcgcggcagg cgctaatgaa caatcaacgc ctgagatttt cactcgtcag
     4261 tgtgaacaca attttcccct caccctaacc cttttcccga aggggcgagg ggactgtccg
     4321 agcacttttg ggccgttttc atcagtctga ggcgttaatc agcgcccctt ctttttacgc
     4381 cccggcgagg taaaacgctt accgttggac gccgggcgac cgcctttttc cgccgttttt
     4441 tccatcttaa cgactggacg tttgatgccc gctgttttcg gtttggcctt cgctttgggc
     4501 ttcgcctcgg aagaggaatt ttcaatgagc ttaaagagat cgataagctc gtcgtcggtt
     4561 aaatcgcgcc attctcccag cggaatgccg cttaagctga cgttcataat gcgcgtacgt
     4621 tccagctttt tcacttcata gccgaaatgc tcgcacatgc gccggatctg acggttcaac
     4681 ccctgcacca gggtaatgcg gaagacaaac ggtgcttctt ttttgacttt gcactttttc
     4741 gtcaccgtcc cgaggatcgg cactcccgcc cccataccac gaataaattc atcggtaatc
     4801 ggtttatcga ccgtcaccag atactctttc tcatgatcgt tacccgcacg gaggatctta
     4861 ttcaccagat cgccgtgatt ggtgaggaaa atcagcccct gggagtcttt atccaggcgg
     4921 ccaatcggga acacgcgttt gctgtggtta acgaaatcga caatgttatc gcgctcgcca
     4981 tcttcggtgg tgctgacaat accgacgggc ttgttcaggg cgataagtac caaatcttcg
     5041 gcttcccgag gctcaatcaa ctgaccgttt actttcacaa tgtcgccggg tttcacctga
     5101 tcaccaatgg tggctcgctt gccattaagg aacacattgc cttgctcgat atagcgatcc
     5161 gcttcgcggc gtgagcaaat tccgctttcg ctgatgtatt tatttaaacg gactgatgag
     5221 tcgggcagca taaattctcc tgtaaaacgc ggaatatacc gtacctctta gcggggaaaa
     5281 acagggttat gcggcaacgt cgaaattaaa tgtgatctgc cacgcctttt atcacaaaag
     5341 tgtctccgga agtacgatgc aaatttgcct ggcaaaataa ggataatcaa catattacga
     5401 atcgctgcac acctgtgctg atgactacta cgcagagaaa caaatgtgca agagaaagcg
     5461 ctatttctcc ccatgcaaag agtgaaaaat cgctttgaaa ggccctcata atggggttat
     5521 atcagatcaa gcggtgagaa agcgtatttg cacaaatgtg cagaaccagg aggcggcaat
     5581 ggaaaacagt gacgatatcc gtttgattgt gaagattgcc caactctatt acgaacagga
     5641 tatgacgcag gcgcaaatcg cgcgcgaact gggtatttac cgcaccacca tcagccgctt
     5701 gcttaaacga ggccgcgatc agggaattgt caccatcgcc atcaactatg actacaacga
     5761 aaatctctgg ctggagcagc aactgaagca aaagtttggc ttgaaagacg ttgtggtggt
     5821 gtcgggaaat gatgaggatg aagagactca actggcgatg atggggttac acggcgcgca
     5881 actgctggat cgcttgctgg agcctggcga tattgtcggt ttttcctagg gtcgcgcggt
     5941 gagcgcactg gttgaaaact tgccgcaagc ggggcaatcg cggcagttaa tctgcgtgcc
     6001 aattattggc ggaccgtccg gtaaactcga aagccgctat cacgtaaaca cattaaccta
     6061 cagcgtggca gcgaagctga aaggggaatc gcatctcgcg gattttccgg ctctgctgga
     6121 taacccctta attcgtaatg ggatcatgca gtctcagcac tttaaaacca tctctgccta
     6181 ctgggataat ctggatgtcg ccctggtggg aattggctca ccggccattc gcgacggcgc
     6241 taactggcat gcgttttatg gtggtgaaga gagtgacgac ctgaaagcag gcgcggtatt
     6301 ccattggcat tgccatgagt gaagaaaaat acagcggaat tgttggtgca ctgcgtggaa
     6361 aatatattaa ttgtctggta acgaatagca gcacagctga actattactg aaataaaaat
     6421 cttcccgatg tttaccgtaa taaaaattac ggcgggtccc tattatctaa aatcaggagt
     6481 ctgttatgca aacgtggtta aatttgcagg ataaaatcat tatcgtcacc ggcggcgcat
     6541 ccggtattgg tctggcgatt gtagaggaat tattagcaca aggcgcaaac gtacagatgg
     6601 tagatattca cggtggcgat ggtcaatatg aagttcataa aggctatcag ttttggccga
     6661 ccgatatttc cagcgccaaa gaggtaaatc atacggtagc ggaaattatc cagcgttttg
     6721 gtcgcatcga cggtctggtc aataacgccg gggtcaattt cccgcgtctg ctggtcgatg
     6781 agaaagcgcc tgccgggcag tatgaactca acgaagctgc attcgaaaaa atggtcaata
     6841 tcaaccaaaa aggcgttttt ctgatgtcgc aggcggtggc gcgccagatg gtcaaacaac
     6901 atgatggcgt gattgtgaat gtttcctcag aaagtggact ggaaggctca gaaggccaaa
     6961 gctgttacgc cgctaccaaa gccgcgctca atagcttcac gcgctcctgg tcgaaagagc
     7021 tgggtaaaca cggtatccgt gtggtcggta tcgcgccggg gattctggaa aaaaccgggc
     7081 tacgcacgcc ggaatatgaa gaggcgctgg cgtggacgcg caatatcacc gtcgagcagc
     7141 tgcgtgaagg ctataccaaa aacgccattc ctattgggcg cgccggaaga ttagcagaag
     7201 tcgctgattt tgtttgttat ctgctgtctg aacgcgccag ctacatcacc ggagtaacca
     7261 ctaacattgc gggcggcaaa acgcgcggat aaggaggctt tatggtcaac gccatttttt
     7321 gtgcccacgg caaattagct tgcgccatgc tggaatcggt tcagatggtg tacggcgacg
     7381 ccaatgtcga ggcggttgaa tttgtaccag gcgagaacgc cggagacatc gtagcaaagc
     7441 tggaaaagtt agtaagcatt cacaatcaag atgagtggct gattgccgtg gatttacagt
     7501 gcggcagccc gtggaatgcg gcagcgatgc ttgcgatgcg caatcctcgt ctgcgggtaa
     7561 tcagcggcct ttccctgccg ctggcgctcg aactggttga taaccaggac agcatgaatg
     7621 ttgatgaact gtgtgaacac ctgacgcaaa tcgcaaagca aacctgcgtt gtctggaaac
     7681 aactggcaac ggcagaggaa gatttctgat gaacattacg ctcgcccgca ttgatgaccg
     7741 tctgatccac ggccaggtca ctaccgtctg gtcgaaagtg gctaacgccc agcgcattat
     7801 tatctgcaat gacgaagttt ataacgatga agtgcgccgg acattattgc gtcaggctgc
     7861 gccaccggga atgaaggtta acgtggtcaa tattgaaaaa gccgtcgcgg tttatcacaa
     7921 cccgcaatat taggacgaaa ccgtttttta tcttttcact cgcccacagg atgccttagc
     7981 gatggttcgc cagggggtaa aaataggcac cctgaatatt ggcggcatgg cctggcggcc
     8041 cggtaaaaaa cagttaacca aagcggtttc attagatgat gatgatatta acgcatttca
     8101 tgagttaaat aatcttggcg tcataatgga tttacgcgtc gtagcgtcag atccctcaat
     8161 caatattatc gacaaaataa acgaacagtt aatcgcaaat taaataatag cgcctgtgta
     8221 catatgcttt aacaggcagg gatgacgaca cttaaaaggt gacaaatcat ggaaataagt
     8281 accctacaaa ttattgctat atttcttttt tcctgtattg ccggaatggg cagcgtgctg
     8341 gatgaatttc agactcatcg cccgttaatt gcctgtacgg tgattggttt aattctcggt
     8401 gatttaaaaa ccggaataat actcggtggt acgctggaat tgatagctct cggctggatg
     8461 aacgtcggcg cggcgcaatc tccggattct gcactcgcca gcataatctc cgccattctg
     8521 gttatcgttg gtcagcaaag catcgccacc ggaatcgcca tcgcgttgcc tgtggctgcg
     8581 gcaggccaag tgctgacggt gtttgcccgt accatcaccg tagtgttcca gcacgcggcg
     8641 gataaagcag cggaagaggc gcggtttcgc actctcgata ttctgcatgt ctccgcgctt
     8701 ggcgtgcagg cgctgcgcgt tgctattccg gcactgattg tctcactgtt cgtcagcgcc
     8761 gatatggtca gcaatatgtt aaacgccatt cccgaatttg tgacccgtgg actgcaaatt
     8821 gctggcggtt ttatcgtggt ggtcggttac gccatggtgc tacgtatgat gggcgtgaaa
     8881 tatttgatgc ctttcttttt cctcggtttc ctcgcaggtg gctacctcga tctcagcctg
     8941 ctggcgttcg gtggcgtcgg cgtgatcatg gccctggtct acatccagct aaatccacag
     9001 tggcgtaaag ctgaacctca actccaggcc accgcctcca ccgcccttga ccaacttgac
     9061 gattaacgga gccatcatgg aacagagaaa aattacacgc agcgatctgg tgagcatgtt
     9121 tctgcgctcc aacctgcaac aggcgtcctt taactttgaa cgtattcacg gactgggctt
     9181 ttgctacgac atgatccccg ccatcaagcg actttaccca ttaaaagagg atcaggttgc
     9241 ggcgctcagg cgacacctgg tgttcttcaa taccacgcca gccgtatgtg gcccggtcat
     9301 cggcgtcact gccgccatgg aagaggcgcg ggccaacggc gcggaaattg atgacggtac
     9361 cattaacggc atcaaagtcg gtctgatggg accattggca ggagttggcg atccactggt
     9421 ctggggaacg ctgcgcccga ttaccgctgc gctcggcgca tctctggcac tttctggcaa
     9481 catcctcggc ccgctgctgt ttttctttat tttcaatgcg gtgcgtctgg cgatgaagtg
     9541 gtatggccta cagctcggct ttcgcaaagg ggtgaatatc gtcagcgata tgggcgggaa
     9601 tgtgctgcaa aaactcaccg aaggcgcgtc gattctcgga ctgtttgtga tgggcgtgct
     9661 ggtcaccaaa tggacgtcaa tcaacgtacc gttggtggtt tcacaaacgc atgccgccga
     9721 tggctccacc gtcatcatga ccgtgcagaa cattctcgac caactttgcc ctggtttgct
     9781 ggcgctcggt ctgacgctac tgatggttcg tctgctcaac aaaaaaatta acccggtatg
     9841 gctgattttc gccctgtttg gcttagggat tatcggcaat gcgctgggct tcctgtcctg
     9901 attcttcgcc ccggcacgac tgccggggcc atcgctcaac atgaggtggt ttatgaaaac
     9961 aacagctctg cgtctttatg gtaaacgtga tttacgcctg gaaacctttg accttcctga
    10021 aatgcaggag gatgaaatcc tcgcgacggt ggtcactgac agcctgtgcc tctcttcctg
    10081 gaaagaggca acctgggtga aaaccataaa aaagtacccg acgatgtggc gaccaaccca
    10141 atcatcatcg gccacgagtt ttgcggcgat attctggccg tgggtaaaaa gtggcagcac
    10201 aaattccagc cgggtcagcg ttatgtgatt caggccaacc tgaaactccc tgaccgcccg
    10261 gactgccccg gctactcctt cccgtgggtg ggcggcgaag ccacgcatgt ggttattccc
    10321 aacgaggtca tggaacaaga ttgcctgctg gcatacgacg gcgaaaccta ttttgaaggc
    10381 tcgctggttg aaccgctttc ctgcgtgatt ggcgcgttca acgccaacta tcatcttcag
    10441 gaaggtagtt ataaccacac gatggggatt cgcccgcaag ggcgcacgct gatcctcggc
    10501 ggcaccggac caatgggatt gttggcgatt gattatgcgc tgcatggacc agttaacccg
    10561 tcgctgctcg tcattaccga taccgacaac gacaaattga gttatgcgcg caagcactat
    10621 ccatcagaac cgcaaacact gattcattat ctcaatgcca ccgatgcagc atttgatacg
    10681 ttaatggcgc tgagtggcgg tcacggcttc gatgatattt tcgtctttgt gcctaatgaa
    10741 ggactggtga ctctcgcctc ttccttgctg gcgacagatg gttgcctgaa tttcttcgcc
    10801 ggaccgcagg ataaacattt cagcgcgcca attaatttct acgatgtgca ttatgcattt
    10861 acccactacg tgggcacgtc aggcggcaat accgacgaca tgcgcgcggc ggtcaaactg
    10921 attgaagaga aaaaagtgca ggccgcaaaa gtggtaacac atattcttgg gctgaatgcc
    10981 gcgggcgaaa ccacgcttga attgcctgcc gtcggcggcg gcaaaaagct ggtgtatacc
    11041 gggaaatacc tgccgctgac gtcactcacg cagattcagg atcaagcact ggcggcgatt
    11101 ctggcgcgtc atcaggggat ctggtccggt gaggcggagc aatatctgct cactcatgca
    11161 gaggcaattt cccatgatta atcgcgctac ttaactctgc atgtcgctgg ctggtcgccg
    11221 cggcaacttc gcgacctgat gtgcggctga ttgctgaagc ggcccaatgt gcacgagaag
    11281 gataaaaaaa catcagcgcc ttaaggggcg ctcaaattgg tggcaaagta cacattgttt
    11341 atgccggatg cggaataaag ccttattcgg cctacataat ctcgaaaatt caatatattg
    11401 cagttatctg gtaggcctgg taagcgtagc gcatcaggca atgctgtgtt tgtcatcagt
    11461 ctcagcgccc ggtgcaatac gtgacatcgt gagccatgtc agtaagtggt taaattcgca
    11521 gtggtagcat gccttccgtg atttcttact aaccggagaa gtaatggaac tgcttttatt
    11581 gagtaactcg acgctgccgg gtaaagcctg gctggaacat gcactgccgc taattgctga
    11641 acagttgcag ggtcgccgct cagcggtgtt tatccctttc gctggcgtaa cgcagacctg
    11701 ggatgattac acagcgaaaa cggctgcggt tctcgctccg ctgggtgttt ctgtcaccgg
    11761 tattcatagc gttgtcgatc ccgttgccgc gattgaaaat gctgagatcg tgattgtcgg
    11821 gggcgggaat actttccagt tgctgaaaca gtgccgcgag cgcgggctgc tggcaccaat
    11881 tactgacgtg gttaaacgtg gtgcgctgta tattggctgg agcgcaggcg ctaaccttgc
    11941 ttgcccaacc attcgtacca ccaacgatat gccgattgtc gatccgcaag gtttcgatgc
    12001 gctaaatctg ttcccgctgc aaatcaaccc gcacttcacc aacgcgctgc cggaaggcca
    12061 taaaggtgaa acccgtgagc agcgtattcg cgaactgctg gtcgtcgcgc cagaactgac
    12121 gattattggt ctaccggaag gtaactggat cacggtgagt aaaggtcacg ctacgctggg
    12181 tggcccgaac accacttatg tgtttaaggc cggtgaagaa gcggtgccgc tggaagcggg
    12241 tcaccgtttt taagtgaatt gccggatgca tgatccggca tcttgttcat agtgcctgat
    12301 gcgacgcttg acgcgtctta tcaggcctac aggattcaca atattaatac tcgtcccgtc
    12361 cttcatcttc atccggctgt tccagcacac tgtaagccac cgaacaaaac agcgagttca
    12421 gacgctgcat atctcccagt aagcctaaat gtagcgaact ggtttcaatg ctctgcacgt
    12481 tttgctgatg caggcgatcg acgtgggcgt gggaatagcg gcgattaaga atgcgaaagc
    12541 gatgcttgct gcggcgcaaa cgacgagcgc tggtgacatc gccagagaag aacaccgaca
    12601 ttgccagctt taaattactg agcaattgct catagagcgc atccagttcc ttcaacccat
    12661 caagcgaaaa tgcccgccgt gctgccagcg atttatcagc aatttcgctg cccatgcgct
    12721 cgacgatatc ggaagcctgt tcaaggttga gcgacatttc aataatctcc gcccagcgac
    12781 gcgactcttc ttctgccagt tcctcttttg gcattcgcgc cagatacagc ttaatggcgg
    12841 tgtagagaac gttgatatca tctgccagct tacgcagctc tttctcctgc cgtggctcgc
    12901 cgtgcatcac tttattcagc ccttccatca tctgttccat ggcgtcgcca atgcgcaggg
    12961 tttcgcgcgc ggcgttcgcc agagcaagcg tgggcgtatc cagcgcgctg acatccagat
    13021 gtttaggccg tagctgggta tccagttccg gttcatcgcg aataatcgtt ttgcaaaacc
    13081 gtgccatcgg gtcaacaaat ggcagcatga ccaggcaacg tacaaggttg tagaagacgt
    13141 ggaaatagat caccagttcc gcttttggca atgacaactt ccccattgtc tctgccagca
    13201 aatggacaaa cggcaggata atcaggctac ccaccagctt aaacagcaaa ctacccagcg
    13261 cgacacggcg ggctgcggca ttggcggcac tgttgttgag catcgccagc aggccggaac
    13321 cgaggttagc accaatcacc agacagagcg ccacggggaa ggagataatg cctgcggcgg
    13381 tcagagtcgc ggtcagcagt acagcagcaa ggctggagta gctgataatg gcgaacatcg
    13441 cgccaatcag cgcatccagc agaatatcgc cggtcagcga ggcaaagatc acctgaacgc
    13501 cgtttgcctg ggtgatcggc gttacggcct gcacaatcaa ctccagcgcc agcaaaatca
    13561 gcccaagacc aataccgacg cggcccagtt gcccggcgcg tgactgtttg cgtccgagga
    13621 aaaaaatcac gccgataaaa ataagtaacg gtgacagcca ggataagtcg aaggtgagaa
    13681 tacgcgccat tagcgccgtc ccgacatctg cacccagcac aatcaccaga gccggtgcga
    13741 gggctaccag atcctgagcg acgaacgagg tcaccagcat ggtggtggca ttactgctct
    13801 gtaccagtgc ggtaacgccg atccccgcgc aaaaggcgag cggctttttt tcgacgctcc
    13861 ggctaaggac agtacgcaaa cgcgcgccga agacgcgcat tacgccggtt cgaacaatat
    13921 gagtccccca gaccagcagg gcgacggcag aaagcaggtg aagcagcgtt aacacgagtt
    13981 caggttctcc ttatcgttat atgttcctga agcaattgaa tgaccaaagg accggaaaca
    14041 cgctgactgg catctttact gtgccttttg acattcgttt ttccggctaa gattattcca
    14101 gcctgtttcc agtataagtg tttcatatta ataaagagac agggcactca ttgagcgccc
    14161 tgtttgttgt aaggaaaagt gacagatttg tgaatcagtc cgcgtcatac cccagattcg
    14221 gtgccagcca gcgctcaaca tcggagacgc tcataccttt acggcgggca tagtcttcaa
    14281 cctgatcgcg ctgaatttgc gccaccgcgt agtacttgct gtccgggtgg ctgaaatacc
    14341 aacccgaaac cgatgcacca ggccacatgg cgaaagattc tgtgagtttc atgccagtat
    14401 gtttttccac ttccagcagc tcccagatgg tggctttttc cgtatgttcc gggcaggccg
    14461 ggtagcccgg tgccggacgg atgccctggt agttttcgcg gatcagttct tcgttgctga
    14521 ggttctcgtt cggcgcatag ccccagtaga ctttacgcac acgctcatgg agatactccg
    14581 caaaggcttc ggctaaacgg tcggcaagcg ctttcaccat gattttgttg taatcatcgt
    14641 gctgcgcttc aaaggcatca gccagtgcgt cctcttccag cccgccagtc acggcaaatg
    14701 cgccgatgta atctgcttta ccagaaagct tcggcgcaac gaagtcagcg agacagtagt
    14761 tagcgaatcc cgttttttcg gtctgttgac gcagatggtg gctgacgttg atcacatggg
    14821 tgcgcgtttc gtcacggtag atttcaatgt catcgcccac acggtttgcc gggaacaagc
    14881 ccaccacgcc acgcggattc agcgttttct cggcgcttaa tttatccagc atgtcgttgg
    14941 cgtctttaaa cagccgctgc gcctcaacgc ccaccacttc atcttccaga atgcgcggat
    15001 acttcccggc cagcgaccag gtcataaaga acggtgtcca gtcgatgtaa ttacgcagag
    15061 tttcgatgct ggcttcaact tcctgcacgc cgagacggtg cgccaccggc ggcgtgtaag
    15121 cctgccagtc aaaagcgaag tcgttatcgc gcgccgcttc cagcgtgacc ggtggtgtgc
    15181 gcggtttctt gcgcccgtgc tgaatacgta cggtttcata ctccttgcgg gtacgagcga
    15241 caaaatcatc gcgctgagta tcggaaagca gcgccgccac cacaccaacg gtgcgcgagg
    15301 cattctgcac atacaccgtc gggccgctgt agttctgctc aattttcacc gccgtgtgcg
    15361 cttttgaggt cgtcgcgcca ccgatcagta acggaatggt gaagccctga cgctccatct
    15421 ctttcgccac gttaaccatc tcgtccagcg acggcgtgat aagccccgaa agtccaatca
    15481 gatcagcatt cacttcttta gcggtacgga gaattttttc cgcaggcacc ataacgccga
    15541 gatcgacaat ttcgtagttg ttacattgca gcaccacacc aacgatattt ttaccgatgt
    15601 cgtggacgtc gcccttcacg gtggcgatca ccatcttgcc gttggttttg ccctgctctt
    15661 tgctggcttc aataaacggt tcgaggtagg ccactgcctg tttcatgacg cgcgccgatt
    15721 tgaccacctg tggcaggaac attttcccct cgccaaacag atcgccgacc acgttcatgc
    15781 cgtccatcaa cgggccttca atcacttcaa tcgggcgcgt agcctgctgg cgggcttctt
    15841 cggtatcctg ctcgataaac tcagtaatgc ctttgaccag cgagtattcc agacgtttat
    15901 tcacttccca cgagcgccac tccgcctgct gggcgttagc ggtgtcgtcg gttttgctgc
    15961 cgcgatattt ctcggcaagc tccagtaaac gttcagtgcc atcatcgcga cgattaagaa
    16021 tcacatcttc caccgcgtcg cgcagttcag cgggcaggtc gtcgtaaatc gccagttgcc
    16081 cggcgttgac gatccccata tccatgccat tgcgaatagc gtagtagagg aacaccgcgt
    16141 gaatggcttc gcgcaccggt tcgttgccac ggaatgagaa agaaacgtta gatacgccgc
    16201 cggaaatcag cgcgtgcggc agttcgcgtt tgatgtcttc gcaggcgccg ataaagtcct
    16261 gcgcatagtt gttgtgctct tcaatgccgg tggcgaccgc gaagatgttc gggtcgaaga
    16321 tgatatcttc tggcgggaag ccaacctctt cggtgaggat tttgtacgcc cgacggcaaa
    16381 tctcgatttt ccgtgcgcgg gtatcggcct gtccctgttc gtcaaaggcc attaccacca
    16441 ccgctgcacc gtaacgacgc aacagtttcg catgatggat aaaggcgtcg acgccctctt
    16501 tcattgagat agagttaaca atgcctttgc cctgaataca cttcagcccc ttttcgatga
    16561 cctcccattt tgaggagtcg atcataatcg gcacgcgagc gatatccggt tcaccggcaa
    16621 tcagattgag aaaacgcacc atcgccgctt cggcgtcgag catcccttca tccatattga
    16681 tatcgataat ctgcgcgccg ttttccacct gctggcgggc cacatccagt gcctcgctgt
    16741 atttctcttc tttgatcagg cgcttgaact tagcggaacc ggtgacgttg gtgcgttcac
    16801 ccacgttcac aaacaggcta tcttcgccaa tgttcagcgg ctccaggccg gacaaacggc
    16861 aggctacggg gatttccggc agtttgcgcg gcgctaatcc ttctactgca cgactcatcg
    16921 ctgcaatatg ttgtggcgtg gtgccacagc agccgccgac gatattgaga aagcccgctt
    16981 ccgcccattc acgtatttgt tttgccatcg tgtcggcgtc gagatcgtac tcaccaaagg
    17041 cgttgggtag cccggcgttc gggtgcgcgg tgacgtagca ttccgcaatc cgtgacagct
    17101 cctgcacgta ctggcgcagt tcatcgggcc ccagcgcaca gttcaggcca aaggtcagcg
    17161 cttcggcgtg gcgcaatgag ttgtaaaatg cttcggtggt ctgcccggag agcgtgcgcc
    17221 cggaggcgtc ggtgatggtg ccagagatca taatcggcag ctcaacgccc agcgtttcaa
    17281 actccgtttt taccgcaaat accgccgctt tggcgttaag ggtgtcgaaa acggtttcaa
    17341 tcaggatcag atccgcacca ccttccacca gcgctttggt ggactctcga taagccgcca
    17401 ccagcccgtc aaaagtgata ttacgaaatg ccggatcgtt gacgtccgga gaaatagacg
    17461 ccgtgcggtt ggttgggccg agaacaccgg caacgtagcg cggtttctcc ggcgtgcgcg
    17521 cggtccactc gtcagcacag gcgcgcgcca gtttcgccgc cgcaaagttg atttccgccg
    17581 acagggattc catctggtaa tccgccatcg caatggtcgt ggagttgaag gtgttggttt
    17641 cgatgatatc cgcgcccgct tcaaagtagg cgttgtggat agcggcgatc acttccggtt
    17701 tactgagtac cagcaggtcg ttgttgcctt tgaggtcgca tggccagtcg gcaaagcgtt
    17761 caccacgaaa atcggcttcg ttcagtcgat agctctggat catggtgccc ataccgccgt
    17821 ccagcaccag aatacgttca tttaactgcg cacgcagttg ttccactttg ctgctcacac
    17881 ttgctcccga cacgctcaac ccagatttaa taaaaattca acaaaccata ctggcataaa
    17941 cgcatctgtg gtaaaagcga ccaccacgca acatgagatt tgttcaacat taactcatcg
    18001 gatcagttca gcaactattg cattaactaa caataaaaat gaaaatgatt tccacgatac
    18061 agaaaaagga gactgtcatg gtcgcaccca ttcccgcgaa acgcggcaga aaacccgccg
    18121 ttgccaccgc accagcgact ggacaggttc agtctttaac gcgtggcctg aaattactgg
    18181 agtggattgc cgaatccaat ggcagtgtgg cactcacgga actggcgcaa caagccgggt
    18241 tacccaattc cacgacccac cgcctgctaa ccacgatgca acagcagggt ttcgtgcgtc
    18301 aggttggcga actgggacac tgggcaatcg gcgcacatgc ctttatggtc ggcagcagct
    18361 ttctccagag ccgtaatttg ttagcgattg ttcaccctat cctgcgcaat ctaatggaag
    18421 agtctggcga aacggtcaat atggcggtgc ttgatcaaag cgatcacgaa gcgattatta
    18481 tcgatcaggt acagtgtact cacctgatgc gtatgtccgc acctatcggc ggtaaattgc
    18541 cgatgcacgc ttccggtgca ggtaaagcct ttttagccca actgagcgaa gaacaggtga
    18601 cgaagctgct gcaccgcaaa gggttacatg cctataccca cgcaacgctg gtgtctcctg
    18661 tgcatttaaa agaagatctc gcccaaacgc gcaaacgggg ctattcattt gacgatgagg
    18721 aacatgcact ggggctacgt tgccttgcag cgtgtatttt cgatgagcac cgtgaaccgt
    18781 ttgccgcaat ttctatttcc ggaccgattt cacgtattac cgatgaccgc gtgaccgagt
    18841 ttggcgcgat ggtgattaaa gcggcgaagg aagtgacgct ggcgtacggt ggaatgcgct
    18901 gactttttct ggcgggcaga ggcaatattc tgcccatcat acctgagtgg caatagaata
    18961 agggtgtctg ttaatcgcat tgacgccaaa ataacttaat gtcatacact tcacacactt
    19021 ttattaactc agcattattt ttaaacatca aaccacttaa ttattaacaa aatcgcaaga
    19081 tctcattcca ccgaaa
//
LOCUS       DAZPBM010000076        18513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPBM010000076 DAZPBM010000000
VERSION     DAZPBM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.076"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="ACHUZQ_002377"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="ACHUZQ_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1026053.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="ACHUZQ_002378"
     CDS             735..1040
                     /locus_tag="ACHUZQ_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026054.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHUZQ_002379"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHUZQ_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1026055.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="ACHUZQ_002380"
     CDS             1788..2534
                     /locus_tag="ACHUZQ_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1026056.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="ACHUZQ_002381"
     CDS             2534..4630
                     /locus_tag="ACHUZQ_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1026057.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="ACHUZQ_002382"
     CDS             4676..5815
                     /locus_tag="ACHUZQ_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1026058.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="ACHUZQ_002383"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="ACHUZQ_002383"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1026059.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="ACHUZQ_002384"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="ACHUZQ_002384"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR1026060.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHUZQ_002385"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHUZQ_002385"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1026061.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHUZQ_002386"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHUZQ_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1026062.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHUZQ_002387"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHUZQ_002387"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1026063.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHUZQ_002388"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHUZQ_002388"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1026064.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="ACHUZQ_002389"
     CDS             complement(12873..13730)
                     /locus_tag="ACHUZQ_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1026065.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHUZQ_002390"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHUZQ_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1026066.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHUZQ_002391"
     CDS             complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHUZQ_002391"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1026067.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHUZQ_002392"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHUZQ_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHUZQ_002393"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHUZQ_002393"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1026068.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHUZQ_002394"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHUZQ_002394"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1026069.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4547 a         4914 c         4782 g         4270 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataaca
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc agttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctaccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaatac cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gcggccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggcg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAZPBM010000077        18213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPBM010000077 DAZPBM010000000
VERSION     DAZPBM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.077"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHUZQ_002395"
     CDS             15..1091
                     /locus_tag="ACHUZQ_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1026070.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHUZQ_002396"
     CDS             complement(1484..1825)
                     /locus_tag="ACHUZQ_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1026071.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUZQ_002397"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUZQ_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR1026072.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUZQ_002398"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUZQ_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1026073.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHUZQ_002399"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHUZQ_002399"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1026074.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUZQ_002400"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUZQ_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR1026075.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUZQ_002401"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUZQ_002401"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1026076.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUZQ_002402"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUZQ_002402"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1026077.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHUZQ_002403"
     CDS             complement(7167..7826)
                     /locus_tag="ACHUZQ_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1026078.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUZQ_002404"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUZQ_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1026079.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUZQ_002405"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUZQ_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR1026080.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHUZQ_002406"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHUZQ_002406"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1026081.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUZQ_002407"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUZQ_002407"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1026082.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUZQ_002408"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUZQ_002408"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1026083.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUZQ_002409"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUZQ_002409"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1026084.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUZQ_002410"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUZQ_002410"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1026085.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUZQ_002411"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUZQ_002411"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1026086.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHUZQ_002412"
     CDS             complement(17270..18112)
                     /locus_tag="ACHUZQ_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR1026087.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4670 a         4574 c         4393 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaa
//
LOCUS       DAZPBM010000078        17916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPBM010000078 DAZPBM010000000
VERSION     DAZPBM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.078"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            201..879
                     /locus_tag="ACHUZQ_002413"
                     /pseudo
     CDS             201..879
                     /locus_tag="ACHUZQ_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            923..5773
                     /locus_tag="ACHUZQ_002414"
     CDS             923..5773
                     /locus_tag="ACHUZQ_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR1026088.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6141..7796
                     /gene="lldP"
                     /locus_tag="ACHUZQ_002415"
     CDS             6141..7796
                     /gene="lldP"
                     /locus_tag="ACHUZQ_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1026089.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7796..8572
                     /gene="lldR"
                     /locus_tag="ACHUZQ_002416"
     CDS             7796..8572
                     /gene="lldR"
                     /locus_tag="ACHUZQ_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1026090.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8569..9759
                     /gene="lldD"
                     /locus_tag="ACHUZQ_002417"
     CDS             8569..9759
                     /gene="lldD"
                     /locus_tag="ACHUZQ_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1026091.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9943..10416
                     /gene="trmL"
                     /locus_tag="ACHUZQ_002418"
     CDS             9943..10416
                     /gene="trmL"
                     /locus_tag="ACHUZQ_002418"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1026092.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10469..11290)
                     /gene="cysE"
                     /locus_tag="ACHUZQ_002419"
     CDS             complement(10469..11290)
                     /gene="cysE"
                     /locus_tag="ACHUZQ_002419"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1026093.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11370..12389)
                     /gene="gpsA"
                     /locus_tag="ACHUZQ_002420"
     CDS             complement(11370..12389)
                     /gene="gpsA"
                     /locus_tag="ACHUZQ_002420"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1026094.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12389..12856)
                     /gene="secB"
                     /locus_tag="ACHUZQ_002421"
     CDS             complement(12389..12856)
                     /gene="secB"
                     /locus_tag="ACHUZQ_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1026095.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12919..13170)
                     /gene="grxC"
                     /locus_tag="ACHUZQ_002422"
     CDS             complement(12919..13170)
                     /gene="grxC"
                     /locus_tag="ACHUZQ_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1026096.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13312..13743)
                     /locus_tag="ACHUZQ_002423"
     CDS             complement(13312..13743)
                     /locus_tag="ACHUZQ_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1026097.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13988..15532
                     /gene="gpmM"
                     /locus_tag="ACHUZQ_002424"
                     /gene_synonym="pgmI"
     CDS             13988..15532
                     /gene="gpmM"
                     /locus_tag="ACHUZQ_002424"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1026098.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15566..16825
                     /gene="envC"
                     /locus_tag="ACHUZQ_002425"
     CDS             15566..16825
                     /gene="envC"
                     /locus_tag="ACHUZQ_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1026099.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16829..17788
                     /locus_tag="ACHUZQ_002426"
     CDS             16829..17788
                     /locus_tag="ACHUZQ_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR1026100.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17775..>17916)
                     /locus_tag="ACHUZQ_002427"
     CDS             complement(17775..>17916)
                     /locus_tag="ACHUZQ_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1026101.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4505 a         4632 c         4678 g         4101 t
ORIGIN      
        1 ttagaatcca cgaaaataat atattcatac aaacacagac atcttttgaa acgaatgata
       61 catttttatg atgaatgctt aaaacaatat ttaatcgaca cacatcactg tcattaatgt
      121 aaagcaacat agatcaccaa taatacaatg gacaactcta ccggcattga tcgctggtcg
      181 gaggaaataa ggatttttac atgaacttga agaagatttt tttcagtgct gtcactgttt
      241 cagttctctg cgcattaacg ggatgtgact acattgaaga aggcaagcca gaaagtagtt
      301 tattgaaaca acaagaagaa cattaaaatt gatctacttg aaaaacaaca agcacagcta
      361 aagagtcaac tggaaactat tcaaaaacaa caaactggca taataagcag tacaaagact
      421 ttaactcacg taattaaatc cgttaaagat caacaaaaca cttttatttt taccgagttt
      481 aacccagcaa aaacgaagta tttcatcctg aacaatggtt cggtggcttt agcgggtcga
      541 gtgttatcca ttgacgccac agagaatggc agtgttattc atatttcact ggtcaactta
      601 ttaagtacac ctatctcaaa tattggtttt aatgcgacat ggggtggtga aaaacctgtc
      661 gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat gaaatccaca
      721 ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg tgtatcgccc
      781 aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt cgacaatctt
      841 ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt acgcttataa
      901 caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga accctgcgac
      961 agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat ctgggcgcag
     1021 taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg gggcattggc
     1081 gaatgccggg aatgacaacg gccagggtgt tgattacggt agtggatcag ctggcgacag
     1141 ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca gtggttccag
     1201 tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca tcgggtcaaa
     1261 aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat cagaaggcga
     1321 tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa tggccctcgg
     1381 ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt ccaaagcgga
     1441 aggagcaaac gccattgccc tgggaaatgc cactaaagct actgagatta tgagtattgc
     1501 tcttggcgac accgccaatg cgtcaaaagc gtattcaatg gcgctgggag caagtagcgt
     1561 cgcatctgaa gaaaacgcaa ttgccctggg gcgtagcagt gtagctagcg gtactgacag
     1621 cctcgcattt ggcagacaat cacttgccag cgcagcgaac gctattgcga taggtgctga
     1681 gaccgaagcc gctgaaaatg caactgctat tggcaataat gcgaaggcaa aagggactaa
     1741 tagcatggca atggggttcg gaagccttgc cgataaagtc aatactatcg cattaggaaa
     1801 tggcagccag gctctggcag ataatgcaat cgccataggc cagggcaaca aagctgatgg
     1861 cgtggatgcc atcgctctgg gtaatggtag ccagtcgaga ggcttaaaca ccattgcctt
     1921 aggcacagcc agtaatgcaa ctggtgataa gagtcttgcg cttggtagta atagcagtgc
     1981 caacggtatt aactctgtcg cgctgggcgc agattccatt gcggatttag acaataccgt
     2041 ctctgtcggc aatagttcat taaaacgcaa gatcgttaat gtgaaaaatg gcgcgatcaa
     2101 gtctgacagt tacgatgcca ttaatggttc acagctttat gccattagcg actcggtagc
     2161 aaaaacgctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta cagcaccaac
     2221 ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg tacttgatga
     2281 aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg gtactagtag
     2341 cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct ccagtaagga
     2401 tgcggttaac ggttcccaac tgaaagctac caatgacgat gtcgaagcca acaccgccaa
     2461 tatcgctact aataccagca acattgccac gaatacagca agtattgcca ccaataccac
     2521 caatatcacc aacctgacgg attccgttgg tgaccttcag gctgatgccc tgctctggaa
     2581 cgaaactaaa aaggcattca gtgcagctca cggccaggat accaccagca aaatcaccaa
     2641 cgttaaagat gccgacctga cggctgacag cactgatgct gttaacggct ctcagctgaa
     2701 aaccaccaac gatgctgtgg cgacgaatac caccaatatc gccaataaca cttccaatat
     2761 tgccactaac accaccaaca tctctaatct gactgagacg gtgactaatc ttggtgagga
     2821 tgcgctgaaa tgggataagg acaatggtgt attcacggca gctcatggca ccgagaccac
     2881 cagcaaaatc accaacgtta aagatggcga cctgacgact ggcagcaccg atgccgttaa
     2941 cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg aataccacca atatcgccac
     3001 taacaccacc aacatctcta atctgactga gacggtgact aatcttggtg aggatgcgct
     3061 gaaatgggat aaggacaatg gtgtcttcac tgcagctcat ggcaacaata ccgccagcaa
     3121 aatcaccaat atcctggacg gcacagtcac tgcaaccagt tccgatgcca ttaacggtag
     3181 ccagctttat gacttaagca gcaatatcgc cacctacttc ggcggcaatg cttctgtgaa
     3241 tactgacggt gtgtttaccg gtccaaccta caaaatcggt gaaacaaatt attataacgt
     3301 cggcgatgca ctggctgcga ttaactcctc atttagcacg tctctcggcg atgctctgct
     3361 ttgggatgcc accgcaggta aattcagtgc caaacacggt actaatggtg acgcaagcgt
     3421 gatcactgat gtcgcagatg gtgaaatttc agactccagt tctgacgcag taaacggctc
     3481 acaactccac ggcgtgagca gttatgttgt tgatgcgctg gggggtggtg ccgaagtcaa
     3541 tgcagacggc accatcactg cgccgacgta caccattgct aatgctgatt acgataatgt
     3601 cggtgatgcc ctgaatgcta tcgataccac tcttgacgac gctctgctct gggatgcgga
     3661 cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa gataaaactg ccagtgtaat
     3721 cactaacgtc gctaacggtg caatctctgc tgccagcagc gacgcgatta acggctcaca
     3781 actctatacc accaataagt acatcgctga tgcgctgggt ggtgacgcag aagtcaacgc
     3841 tgacggcacc atcaccgcac cgacttacac cattgcgaac gccgagtaca acaacgtcgg
     3901 tgacgccctg gatgcgcttg atgataacgc cctgctgtgg gatgagactg ccaatggcgg
     3961 tgctggagcc tacaatgcca gccatgacgg taaagccagc atcatcacta atgtcgctaa
     4021 tggcagtatt agtgaggaca gtaccgatgc agtgaacggt tctcagttga atgcgacgaa
     4081 tatgatgatt gagcagaaca cccaaattat caatcagctc gctggtaaca ccgacgcaac
     4141 ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt actaacgaca acgatttagc
     4201 ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct gtaggttata acgctgtcgc
     4261 gtctggtgcc agcagcgtag ccattggtca gaacagcagc agcaccgttg ataccggtat
     4321 tgcgctgggt agcagctccg tttccagccg tgtgattgcc aaaggttctc gtgacactag
     4381 cgtaacggaa aatggcgtgg tgattggtta tgacaccact gacggcgaac tgctaggtgc
     4441 attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc aacgtagctg atggttcaga
     4501 agcccatgac gccgttacgg ttcgccagtt gcagaacgct attggagcgg tcgccactac
     4561 gccaaccaag tactttcacg ccaactcaac ggcagaagac tcactggccg ttggtgaaga
     4621 ctcgctggca atgggtgcga aaactgtcgt taatggtaat gcaggtattg gtatcggttt
     4681 gaacactctg gttctagctg atgcgatcaa cggcattgct atcggcagta acgcacgcgc
     4741 aaaccatgca aacagtatcg caatgggtaa cggttctcag accacccgtg gtgcacagac
     4801 tggctacacc gcctacaaca tggacgcacc gcagaactct gtaggtgagt tttctgtcgg
     4861 tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct ggttcagcgg ataccgatgc
     4921 ggttaacgtg ggtcagttga aagtcactga tgagcgcgta gcgcaaaata cccagagcat
     4981 tactaacctg aacaatcagg tcactaatct ggatactcgc gttactaata tcgaaaacgg
     5041 tattggcgac attgtcacca ccggtagcac caagtacttc aagaccaaca ccgatggcgt
     5101 agatgccaac gcccagggta aagatagcgt tgctattggt tctggttcca ttgctgccgc
     5161 tgacaacagc gtcgcactgg gtaccggttc cgttgcagag gaagaaaata caatctctgt
     5221 aggttcttct actaaccaac gccgtatcac caacgtagct gccggtgtta atgccaccga
     5281 tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc ggcgtgcgct acgacaccaa
     5341 agctgatggt tctatcgact atagcaatat caccctcggt ggtggcaacg gcagtacgac
     5401 tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac gcggtgaact acgcgcagtt
     5461 gaagcaaagc gtgcaggaaa cgaagcaata caccgatcag cggatggttg agatggataa
     5521 caaactgtct aaaactgaaa gcaagttgag cggtggtatc gcttctgcaa tggcaatgac
     5581 cggtctgccg caggcttata caccgggtgc cagcatggct tctattggtg gcggtactta
     5641 caacggtgaa tcggcagttg ctttaggtgt atcgatggtg agcgccaatg gtcgttgggt
     5701 ctacaaatta caaggtagta ccaatagcca gggtgaatac tccgccgcac tcggtgccgg
     5761 tattcagtgg taatgatcca ttaagttagt gtgactaagg gcaaacgcca aattgcctga
     5821 tgcgttacgc gcatcaggca agataaggcg ccccgggtca acaatctaag ggcaattctc
     5881 tgatgaggat tgcccttttc tttaccagac atctcccccc acaagaattg gccctaccaa
     5941 ttcttcgctt atctgacctc tggttcacaa ttccccaatt aaaactcaca tcaatattgc
     6001 caatacataa catttagtta accattcatt gtcattatcc ctacacaaca caattggcag
     6061 tgccactttt acacaacgtg tgacaaggag atgagcaaca gactcattac acgatgtgcg
     6121 tggactccag gagacctgca atgaatctct ggcaacaaaa ctacgatccc gccgggaata
     6181 tctggctttc cagtctgata gcatcgcttc ccatcctgtt tttcttcttt gcgctgatta
     6241 agctcaaact gaaaggatac gtcgccgcct catggacggt agcaatcgcc cttgccgtgg
     6301 ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc ctcggtggtt tatggcttct
     6361 tctatggttt gtggcccatc gcgtggatca ttattgcagc agtgttcgtc tataagatct
     6421 cggtgaaaac cgggcagttt gacatcattc gctcgtctat tctttcgata acccctgacc
     6481 agcgtctgca aatgctgatc gtcggtttct gtttcggcgc tttccttgaa ggagccgcag
     6541 gctttggcgc accggtagca attaccgccg cattgctggt cggcctgggt tttaaaccgc
     6601 tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc agtggcattt ggcgcgatgg
     6661 gcattccaat cctggttgcc ggacaagtaa caggcatcga cagctttgag attggccaga
     6721 tggtggggcg gcagctaccg tttatgacca ttatcgtgct gttctggatc atggcgatta
     6781 tggacggctg gcgcggtatc aaggagacgt ggcctgcggt cgtggttgcg ggcggctcgt
     6841 ttgccatcgc tcagtacctt agctctaact tcattgggcc ggagctgccg gacattatct
     6901 cttcgctggt atcactgctc tgcctgacgc tgttcctcaa acgctggcag ccagtgcgcg
     6961 tattccgttt tggtgatttg ggggcgtcaa agattgatat gacgctggcc cacaccggtt
     7021 acactgcggg tcaggtgtta cgtgcctgga caccgttcct gttcctgaca gctaccgtaa
     7081 cactgtggag catcccgccg tttaaagccc tgttcgcatc gggtggtgcg ctgtatgagt
     7141 gggtgatcaa cattccggtg ccgtacctcg ataaactggt tgcccgtatg ccaccagtgg
     7201 tcagcgaggc tacagcctat gccgccgtgt ttaagtttga ctggttctct gccaccggca
     7261 ccgccattct gtttgctgca ctgctctcga ttgtctggct gaagatgaaa ccgtctgacg
     7321 ctatcagcac cttcggcagc acgctgaaag aactggctct acccatctac tccatcggca
     7381 tggtgctggc gttcgccttt atctcgaact attccggact gtcatcaaca ctggcactgg
     7441 cactggcgca caccggtcat gcattcacct tcttctcgcc gttcctcggc tggctggggg
     7501 tattcctgac cgggtcggat acctcatcta acgccctgtt cgccgcgctg caagccaccg
     7561 cagcacaaca aattggcgtc tctgatctgt tgctggttgc cgccaatacc accggtggcg
     7621 tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc ctgtgcggcg gtaggcctgg
     7681 tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca cagcctgatc ttcacctgta
     7741 tggtgggcgt gatcaccacg cttcaggctt atgtcttaac gtggatgatt ccttaatgat
     7801 tgttttaccc agacgcctgt cagacgaggt tgccgatcgt gtgcgggcgc tgattgatga
     7861 aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc caactggcga tgcaactcgg
     7921 cgtatcacgt aattcactgc gcgaggcgct ggcaaaactg gtgagcgaag gcgtgctgct
     7981 cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat gacacatggt cggagcaaaa
     8041 catcgtccag ccgctaaaaa cgctgatggc cgatgatccg gattacagtt tcgatattct
     8101 ggaagcccgc tacgccattg aagccagcac cgcatggcat gcggcaatgc gcgccacacc
     8161 tggcgacaaa gaaaagattc agctttgctt tgaagcaacg ctaagtgaag acccggatat
     8221 cgcctcacaa gcggacgttc gttttcatct ggcgattgcc gaagcctcac ataacatcgt
     8281 gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa tcctcagtga agcatagccg
     8341 tcagcggatg tatctggtgc caccggtttt ttcacaactg accgaacaac atcaggctgt
     8401 cattgacgcc atttttgctg gtgatgccga cggggcgcgt aaagcaatga tggcgcacct
     8461 tagttttgtt cacaccacca tgaaacgatt cgatgaagat caggctcgcc acgcacggat
     8521 tacccgcctg cccggtgagc ataatgagca ttcgagggag aaaaacgcat gattatttcc
     8581 gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc cgccgttcct gttccactat
     8641 atggatggtg gtgcatattc tgaatacacg ctgcgccgca acgtggaaga tttgtcagaa
     8701 gtggcactgc gccagcgtat tctgaaaaac atgtccgact taagcctgga aacgacgctg
     8761 tttaatgaga aattgtcgat gccggtagca ctggcaccgg tgggtttgtg tggcatgtat
     8821 gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg cgcatggcat tccgtttact
     8881 ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc cagccatcaa gcgcccaatg
     8941 tggttccagc tttatgtact gcgcgatcgc ggctttatgc gtaacgcgct ggagcgagca
     9001 aaagcagcgg gttgttcgac gctggttttc accgtggata tgccgacacc gggcgcacgc
     9061 taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg caatgcgccg ctacttgcag
     9121 gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga acggtcgtcc acatgattta
     9181 ggtaatatct cagcttatct cggcaaaccg accggactgg aagattacat cggctggctg
     9241 gggaataact tcgatccgtc catctcatgg aaagaccttg agtggatccg cgatttctgg
     9301 gatggcccga tggtgatcaa agggatcctc gatccggaag atgcgcgcga tgcagtacgt
     9361 tttggtgctg atggaattgt ggtttctaac cacggtggcc gccagctgga cggtgtactc
     9421 tcttccgctc gtgcgttgcc cgctattgca gatgcggtga aaggtgatat cgccattctg
     9481 gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta tgattgcgct cggtgccgac
     9541 accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa cagcgggcca ggcgggtgta
     9601 gctaacctgc taaatctgat cgaaaaagag atgaaagtgg cgatgacgct gactggcgcg
     9661 aaatcgatca gtgaaattac gcaagattcg ctggtgcagg ggctgggtaa agagttgcct
     9721 acggcactgg ctccgatggc gaaagggaat gcggcatagt cgtttgcgcc ccctcaccct
     9781 cagggagagg ggaccgttcg gcgctgtatg tactccctca ctctgaaacg acaccgcact
     9841 ctttttttct ccctcgcccc tccggggagg gccggggtga ggggaaaagg ccgcacggtt
     9901 caccaatttt ctgctatcct gcccccgcac taagggggca gtatgctaaa catcgtactt
     9961 tacgaaccag aaattccgcc aaatactggc aacatcatcc gtctttgcgc taataccggc
    10021 tttcgtctgc atatcatcga accgatggga tttgcctggg acgataagcg cctgcgccgc
    10081 gcggggctgg actatcacga gtttaccgcc gttacgcgtc atcatgacta tcgcgcgttc
    10141 ctcgaagcag aaaatcccca gcgcctgttt gccctcacca cgaaaggtac gcctgctcac
    10201 agcgccgtaa gctatcagga tggcgattat ctgatgttcg gcccggaaac acgcggcctg
    10261 ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa ttcgcattcc gatggtgccg
    10321 gacagccgca gcatgaatct gtccaatgcg gtgtcggtag tggtgtatga agcctggcgg
    10381 cagctgggat atccgggagc gttgttgaga gattagttac tgtatgccgg atagatgatt
    10441 acatcgcatc cggcacgatc acaggacatt agatcccatc cccatactca aatgtatggt
    10501 taataccgtt gaaatgctgg tccatatcca ttgatggctt atcgctgtct ggtttaccga
    10561 caatacgagc cggaacgcca gcggcggttg tatgcggcgg caccggttgc agcaccacgg
    10621 aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt gccgaggatt ttcgcgcccg
    10681 cgccaatcat cacaccttca cgaattttcg ggtgacggtc accaccagat ttacccgtac
    10741 cgccaagcgt cacagattgc agaatcgata cgtcgttttc aattaccgcc gtttcaccca
    10801 caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc aatttttgct gccgggtgaa
    10861 tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa gattgccaat gcgcgacgcc
    10921 cctgattcca caaccagtga ccgatgcgat aggcctgcaa ggcatgaaaa cccttcaggt
    10981 ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg ggtacgcacc gcctgaatat
    11041 cacaggccgc agaggcgatc atttccgggt cagcggcgta ggcttcttcc accacttcac
    11101 ggatagcaat agcaggcata attggcgatg acagcttgtt cgccagcatg tagctcagtg
    11161 cactaccaag gttttcgtgc ttgagtagcg tcgcgtggta aaaactggcc agcattggct
    11221 cacagtccgc cagcgttctg gcttcggctt taatattgtt ccagacaatt tccagttctt
    11281 cacacgacat tgcttactcc acacgatgag ataatgaccg gcccgttctg cgcgggccgg
    11341 gtcatagcgg taacaaaggt tccctggggt tagtggctgc tgcgctcgtc cttgcgtgca
    11401 cgacctaata acgtcaatgc tgcctcgcgc gcgttttttc cgcaatataa tacttgataa
    11461 atttcctcgg ttattggcat ttctacgccg aagcgatgcg ccagttcgcg gacttctttc
    11521 gtattgcggt agccttccac cacctgacca atcttctcct gcgcgctttg tacatccatg
    11581 ccctgaccga gcatcatgcc aaaacggcgg ttacgcgact ggttgtcggt acaggtaagc
    11641 accagatcgc ccagccccgc catgcccata aaggtggcag gatcggcacc cagcgccgcg
    11701 ccaagacgcg acatttcagc cagcccacgg gtgatcagtg ccgtacgcgc attcgcacca
    11761 aaaccgatac cgtcggacat ccccgcgcca atggcaatga cgtttttcac cgcaccgcca
    11821 agctgcacac cgatgaaatc cgggttgctg taaacgcgga aacttttgcc acagtgcagc
    11881 aattgttgga gatcatcggc aaaggtctgg tcggtagagg ccaacgaaat agccgtcggt
    11941 aaacctgccg ccagttcttt cgcaaacgtt gggccagaga taaccgccag cggaatttga
    12001 tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc cggtttccgc ttccagccct
    12061 ttggtcgccc acaccagacg cgcatcagga cgcatcagcg gtttaatctg gcgcagcact
    12121 tcaccaaaga catggctggg tacgacgacg agaatattac ggctggctgc cagcgcagtg
    12181 gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca catcggggag aaacgcggcg
    12241 ttacagcggt cgcgttcaag cgttgcgata tgttcagggt catggcccca gaggacaacc
    12301 tcgtggccat ttcttgccag ggtaatggca agagcggtgc cgtacgagcc ggcaccgatc
    12361 acagtcattg aagcattacg ttggttcatc aggcatcctg atgttcttca gtaccttcgc
    12421 cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa gttaatcggc gcaaggttca
    12481 gttgcgggaa tgtaccgcgg gataccacgc tggtgatgca ctcacgagca tacgggaaca
    12541 gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg ggtgccttcg atacccgcga
    12601 tggagaaaat accgccctgc tgaacttcac acaggaacgc ggtttcttcg cccagggagg
    12661 ccgttacggt aacacgcagt accacttcgt atacgtcatc tgccagttgg gaagatgccg
    12721 tatccagatc aagtttaact tctggttgcc aatctttctg gaaaacgtgc ggcgcgttcg
    12781 gcgcttcgaa agagatatcc ttggtataaa tacgttggat ctggaaagtc atttcagtgt
    12841 tgttttgttc tgacatgtgt agaaaaccct taagtgttgt ccttaaatac tgcgcaatgt
    12901 aatgccaacg ttcacacgtt atttcagcag gggatccagt ccaccacgtg catccaatgc
    12961 atacaagtca tcacagccgc caatgtgctg tgcgtcaata aaaatctggg gaaccgtggt
    13021 gcgaccgctg cgtttgatca tctcttcacg cttggcggca ttgccatcga tcggcaactc
    13081 ctgaaaactc acgcccttgc tgctcagcag tgcttttgca cgatggcaat acgggcaggt
    13141 ttctttggta tagatttcaa cattggccat aatttagctc ctgttttttt accctgaaat
    13201 ttcatgttgc agggcggctg caaaatggag tgtccccaga agcttataac aataagtcgc
    13261 tgggggccta attgcagcta acggcctgca tcatgaaaga cgacaggtaa attatttgcc
    13321 gcgcaccaga ggcaagtttt cgccagccca gccagcaacg ccctctttca gcacgaatac
    13381 ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc tggcactgca tgccagaacc
    13441 gtctaccacg ataaccggtt tgtctttgtg cttctcaagc tcaccaacat tgttggcttt
    13501 gatttcgctc ggcaacagat tgatagaacc tgcgatatgg cctttacgga agtcatcacg
    13561 ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc agacgcgtag cttcaccacg
    13621 agtaatcacc ttcactttcg aggtcaggct cttaaacgta gtcacaagaa ccgccaccag
    13681 taacgcgatc caggcgatac tcagtatggg atgacggcca acaaattgca taatttcttg
    13741 catggggggt aacaactccc gacgtagtga ttaaaaaaac caggaaagga gtatacctgc
    13801 gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca attttgcggg gcacgacgaa
    13861 aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc gtatattttg atcttcagag
    13921 gctattttat cgattcagct gtagtaaaat tacgcaaatt ttgactcttg agtatgaggt
    13981 tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg attctggatg gctatggcta
    14041 tcgtgaagaa cagcaggata acgccatttt tagtgctaaa accccggtaa tggatgcact
    14101 gtgggccaat cgtccgcata ccctgatcga cgcttccggt ctggaagtcg gtctgcctga
    14161 ccgtcagatg ggtaactccg aagtaggcca cgttaacctg ggtgccggcc gcatcgtgta
    14221 tcaggacctg actcgtctgg acgttgaaat caaagatcgc gctttctttg ctaatccagt
    14281 gctgactggc gcggtagata aagcgaaaaa cgcaggtaaa gcagtacaca ttatgggtct
    14341 gctctccgca ggcggcgtac acagccacga agatcacatc atggcgatgg tagaactggc
    14401 agctgaacgc ggcgcagaaa aaatctacct gcacgcattc cttgacggtc gcgacacccc
    14461 gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag aaatttgctg cgctgggtaa
    14521 aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg gaccgcgata accgttggga
    14581 tcgcgttgaa aaagcctatg acctgctgac tctggcgcag ggtgagtttc aggccgatac
    14641 tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa aacgacgagt tcgtgaaagc
    14701 gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg gaagacggcg atgcgctgat
    14761 tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact cgtgcattcg tgaacgctga
    14821 tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat ttcgtgatgc tgaccgaata
    14881 cgccgctgac atcaaaactg cggttgctta cccacccgct tccctggtta acaccttcgg
    14941 cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt tccgaaaccg aaaaatatgc
    15001 ccacgttact ttcttcttca acggtggcgt agaagagtcg ttcaaaggcg aagatcgcat
    15061 tctgatcaac tcaccgaaag tggctaccta cgatctgcaa ccggaaatga gctccgcaga
    15121 gctgaccgaa aaactggttg cggccatcaa gagcggcaaa tacgacacca ttatctgtaa
    15181 ctatccgaac ggcgacatgg taggtcacac cggggtgatg gaagcggcgg ttaaagcggt
    15241 tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg gttgagtccg tgggtggaca
    15301 actgctgatc accgctgacc acggtaacgc tgaacagatg cgcgatccgg caacgggtca
    15361 ggcacacacg gcacacacca acctgccagt tccgctgatt tacgttggtg ataagaacgt
    15421 gaaagcggtt gcaggcggca aactttctga catcgcgccg accatgttgt cgctgatggg
    15481 tatggaaatc ccgcaagaga tgactggtaa gccgctgttc atcgtggaat aatccctccc
    15541 catgagggga aaggcgatta ataccatgac acgggccgtg aaaccgcgca ggtttgcaat
    15601 caggcccatc atctacgcca gcgttcttag cgctggcgta ttgttgtgcg ccttttccgc
    15661 ccacgcggat gagcgtgacc aactcaaatc tattcaggcc gatatcgcca caaaagagcg
    15721 cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc gcacaattga aaaagcagga
    15781 agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg caaaacacgc tcaatcaact
    15841 caataaacag attgatgaga tgaacgcgtc gattgccaaa ctggagcagc aaaaagccgc
    15901 ccaggagcgc agcctcgccg cacaactgga tgccgcattc cgtcagggcg agcataccgg
    15961 tattcagcta attctcagcg gtgaagaaag ccagcgtgga cagcgtttac aggcttattt
    16021 cggctatctc aaccaggcgc gacaagaaac cattgcccag ttgaagcaaa cgcgtgaaga
    16081 agtcgccatg cagcgtgctg aactggaaga gaaacaaagc gagcaacaaa cgctgttata
    16141 tgaacagcgc gcccaacagg cgaaactgac tcaggctctg aacgagcgta aaaagacgct
    16201 ggcggggctg gaatcttcca tccagcaagg tcagcagcag ctaagtgaac tgcgtgccaa
    16261 cgaatcccgt ctgcgtaaca gtattgcccg tgcggaagcc gcggcgaaag cgcgtgctga
    16321 acgagaagcg cgtgaggctc aggcagttcg cgacaggcag aaagaagcga cgcgcaaagg
    16381 caccacctac aaaccgaccg aaagcgaaaa atcgctgatg tcccgaactg gtggcctggg
    16441 tgcgccgcgt ggtcaggcat tctggccagt tcgtggccct acgctgcatc gctatggcga
    16501 gcagctacag ggtgaattac gctggaaagg aatggttatc ggtgcctctg aaggtactga
    16561 agttaaagca attgccgacg gtcgggtgat tctggctgac tggctgcaag gctacggtct
    16621 ggtggtggtg gttgagcacg gtaaaggcga tatgagtctt tacggctata atcaaagcgc
    16681 gctggtgagc gttggttcgc aggttcgtgc gggccagcca attgcactgg tgggcagcag
    16741 tggcggtcag ggcagacctt cactctattt cgaaattcgc cgccagggtc aggccgtcaa
    16801 tccacagccg tggttgggaa gataagtttt gtttccattt cgtcgtaacg ttcttgcatt
    16861 tgccgctctg ttggcgctct cctcccctgt aattgctggc aaacttgcca tcgtcattga
    16921 tgattttggg tatcgcccga acaacgaaaa ccaggtgctg gcgatgcctt ccgctatctc
    16981 cgtcgctgta ttacccgatt caccgcacgc cagagagatg gcgaccaaag cgcataacag
    17041 cggtcacgaa gtgttgattc atctcccgat ggcaccgttg agtaaacagc cgctggagaa
    17101 aaatacgctg cgcccggaga tgagcagcga cgaaattgag cgcattattc gtagtgcggt
    17161 caataacgtg ccctatgccg tggggatcaa taaccacatg ggcagcaaga tgacctctaa
    17221 cctgtttggt atgcagaaag tgatgcaggc gctggagcgt tataatcttt acttcctcga
    17281 cagcgtaacc attggtaata cccaggcgat gcgcgccgcg caaggcactg gcgtgaaggt
    17341 gatcaaacgg aaggtgttcc tcgacgattc acagaacgaa gcggacatcc gtgtgcaatt
    17401 caatcgcgca attgacctag cgcgtcgcaa cggatcaacc attgccattg ggcatcctca
    17461 cccttcaacg gtacgcgtgt tgcaacagat ggtttataac ctgccgccag acattacgct
    17521 ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat acgtctacac cgccgaaaaa
    17581 cactgtgccc gacacaccgc gtaatccgtt ccgtggcgtg aagctgtgca aaccgaagaa
    17641 accgatagaa cctgtttatg ctaatcgctt ctttgaagta ttaagcgaaa gcatcagcca
    17701 gagcacgctg atcgtttact tccagcatca gtggcaaggc tggggcaaac agcctgaagc
    17761 ggcgaagctt aacgctagcg taaattaaag gcgctacgcg taatgcgatg gtgcgaccgc
    17821 gttttgtcgc gccattgcca taagcggaaa gaccacagta acgcctgata acccaccttt
    17881 acgctgcgca cattggtaat caggcgctta tacata
//
LOCUS       DAZPBM010000079        17797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPBM010000079 DAZPBM010000000
VERSION     DAZPBM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.079"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(78..1052)
                     /gene="cysB"
                     /locus_tag="ACHUZQ_002428"
     CDS             complement(78..1052)
                     /gene="cysB"
                     /locus_tag="ACHUZQ_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1026102.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1262..3859)
                     /gene="topA"
                     /locus_tag="ACHUZQ_002429"
     CDS             complement(1262..3859)
                     /gene="topA"
                     /locus_tag="ACHUZQ_002429"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1026103.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4239..4490
                     /locus_tag="ACHUZQ_002430"
     CDS             4239..4490
                     /locus_tag="ACHUZQ_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1026104.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4526..5575)
                     /gene="sohB"
                     /locus_tag="ACHUZQ_002431"
     CDS             complement(4526..5575)
                     /gene="sohB"
                     /locus_tag="ACHUZQ_002431"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1026105.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5795..6553
                     /locus_tag="ACHUZQ_002432"
     CDS             5795..6553
                     /locus_tag="ACHUZQ_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1026106.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6550..7140
                     /gene="cobO"
                     /locus_tag="ACHUZQ_002433"
     CDS             6550..7140
                     /gene="cobO"
                     /locus_tag="ACHUZQ_002433"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1026107.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7180..8052)
                     /gene="rluB"
                     /locus_tag="ACHUZQ_002434"
     CDS             complement(7180..8052)
                     /gene="rluB"
                     /locus_tag="ACHUZQ_002434"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1026108.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8265..9379)
                     /locus_tag="ACHUZQ_002435"
                     /pseudo
     CDS             complement(8265..9379)
                     /locus_tag="ACHUZQ_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9407..10027)
                     /locus_tag="ACHUZQ_002436"
     CDS             complement(9407..10027)
                     /locus_tag="ACHUZQ_002436"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1026109.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10024..10905)
                     /gene="rnm"
                     /locus_tag="ACHUZQ_002437"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10024..10905)
                     /gene="rnm"
                     /locus_tag="ACHUZQ_002437"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1026110.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11043..11087
                     /gene="trpL"
                     /locus_tag="ACHUZQ_002438"
     CDS             11043..11087
                     /gene="trpL"
                     /locus_tag="ACHUZQ_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1026111.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11064..11157
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11179..12741
                     /gene="trpE"
                     /locus_tag="ACHUZQ_002439"
     CDS             11179..12741
                     /gene="trpE"
                     /locus_tag="ACHUZQ_002439"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1026112.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12741..14336
                     /gene="trpD"
                     /locus_tag="ACHUZQ_002440"
     CDS             12741..14336
                     /gene="trpD"
                     /locus_tag="ACHUZQ_002440"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1026113.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14337..15698
                     /gene="trpCF"
                     /locus_tag="ACHUZQ_002441"
     CDS             14337..15698
                     /gene="trpCF"
                     /locus_tag="ACHUZQ_002441"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1026114.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15710..16903
                     /gene="trpB"
                     /locus_tag="ACHUZQ_002442"
     CDS             15710..16903
                     /gene="trpB"
                     /locus_tag="ACHUZQ_002442"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1026115.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16903..17709
                     /gene="trpA"
                     /locus_tag="ACHUZQ_002443"
     CDS             16903..17709
                     /gene="trpA"
                     /locus_tag="ACHUZQ_002443"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1026116.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4265 a         4722 c         4456 g         4354 t
ORIGIN      
        1 gtatagaaat aagtaacaaa agagaatata ttccggcacc ttcgctacat aaaaggtgcc
       61 gaaaataacg caagaaatta tttttccggc agttttatat ctttaaacat gacctcaatt
      121 tcttcattag agcgcaatgc gacagccgca tcaacgacat cacgcgttaa atgcggtgca
      181 aaacgctgaa tgaaatcata catataactg cgcaagaaag tactacggcg aaaaccaatt
      241 ttggttgtac tgtggctgaa gatatcgtga gcatcaacac gcacaaggtc gggatcggcg
      301 accggatcca ccgccatgct ggcaatgacc cctaccccca gccctaaccg gacgtaagtt
      361 ttaatgacgt cagcatccgt tgccgtgaag acgatacgcg gcgttaaccc tgcgcggtta
      421 aaggcagtat ccagttctga acgtccggta aagccgaagg tatatgtcac caacggatat
      481 tgcgccagtt cttcaatggt aatggctttt ttgcctgcca gcgggtgatc cggagtgact
      541 acaatagccc gattccagtg gtagcacggt aacatcacta aatcttcata cagatgcagc
      601 gcttctgtgg cgatagcgaa atcagcattg cctttagaga cggcatcagc aatttgtgtc
      661 ggcgagccct ggtgcatatg caaagaaacg cggggataac gctcaataaa gcctttgata
      721 acgtttggta acgcgtagcg tgcctgggta tgcgtggtgg cgatatacag tgagccttta
      781 tccggccagg tgtgctctcc ggcaaccgat tttatggcat cgactttcga caggacttcg
      841 cgagcgatac gaattatttc ttgccctgct ggcgttacct gcgtcaggtg cttgccgctt
      901 cgggaaaaaa tttgaatgcc tagctcgtct tccagcattc tgacttgttt actgatcccg
      961 ggttgtgatg tgtaaagtcc ttccgctgtt gatgagacat tcaggttatg attgaccacc
     1021 tcaacaatat agcgaagttg ttgtaatttc atgttaaacc atccacttag acgccatcag
     1081 aacatcgttt tttcgtcaca cctggcgggt gactaatacg atttaataat aaaaaaggtg
     1141 ctaactataa ccactatatc atttatagcc ttagtgtata ggtgtgaaga aaaaataata
     1201 tgcaataaaa aagggccgct ttcgcgaccc tttgtttata aaaacctgac agaattaaag
     1261 gttatttttt tccttcaacc catttgccat caacataaaa tgctgaccag ccagtcgcct
     1321 ttccgtcttt ttccgaagag acatattgct gtttggtttt acggctaaag cgaaccatgg
     1381 tcttattacc ttccggatcc tgctgtggcg catcggccag ataacgcagt ttttccggca
     1441 gacggtcgcg gaagcgataa agctcttcca ccagtggcgc acgcgtttca cgcgatttcg
     1501 ggaaagtgtt ggcagccagg aaaacacccg cagcgccgtc acgcagcacg aaataagcat
     1561 ctgatttttc gcacggcagc tcaggtaatg gcaccggatc ttctttcggt ggtgccactt
     1621 cgccgttacg taaaatctta cgtgtgtttt tacactcttc gttggtgcag gccatgtatt
     1681 taccgaaacg ccccattttc aggtgcattt cagagccaca tttttcacac tcaacgatcg
     1741 ggccgtcata acctttaatg cggaattcgc cctcttcgat ctcgtaaccg tcgcaggttg
     1801 ggttattacc acagacatgc aacttacgtt tcggatcgat gagatagctg tccatcgccg
     1861 tgccgcattt cgggcaacga cgttttgcgc gcagcgcgtt ggtttcagcg tcttcgcctt
     1921 ccagtacgtt cagcacttcg ttttccggca ccaggttaat ggtggttttg caacgctctt
     1981 tcggcggcag cgcataacca gaacagccaa ggaatacccc ggtgctcgct gtgcgaatcc
     2041 ccattttgcg accacaagtc gggcagtcaa tgctggtcag aaccatctgg ttcgggcgca
     2101 taccaccctc ttccggatct ttttcagctt tatctaactg ctgggtgaaa tccgagaaga
     2161 agtgatccag tacagctttc cactctgctt cgtgatttgc caccaggtcg aggctgtttt
     2221 ccatctgtgc ggtgaagtcg tagttcatta actcgcggaa attttcttca aggcgatcgg
     2281 tgacgatttc gccaattttt tccgcataga aacgacgatt ttctactcgc acgtagccac
     2341 gatcctgaat ggtcgaaatg atcgacgcat aggtagacgg acgaccgata ccgcgttttt
     2401 ccagctcttt aaccagcgat gcttcactga aacgggctgg cggcttggta aagtgctggg
     2461 ctggtgtaag ttcaacgagc gtcagagcat cgcctttatt aactgctggt aagatgcgat
     2521 cttcatcgcc tttacgcaac gcaggcatca cttttgtcca gccatcaaaa cgcaaaatac
     2581 gaccgcgtgc tttcaggcgg aaatcgcccg caccaatggt cagcgtcgtg gagtcatatt
     2641 tcgctggggt catctggcag gctacgaact gacgccagat taactggtac agtttctgcg
     2701 catctgcttc catatccttc agcgattccg ccatcacatt gacgtcagaa ggacgaatcg
     2761 cttcgtgcgc ttcctgtgag ttttctttgc tggcgtactg attcggactt tccggcagat
     2821 atttcttacc aaaattatcg ctgatataac cacgaaccat atttaccgcg tcctgactca
     2881 ggttagtgga gtcggtacgc atgtaagtga tatagcctgc ttcatacaaa cgctgcgcca
     2941 tcatcatggt ttttttcacg ccaaatccaa gacgggtgct ggcagcttgt tgcagcgtag
     3001 aggtaataaa aggagcgcca ggtttactgg ttgtcggttt gtcttcacgt tccagcacgc
     3061 tgtagcgcgc tttttccagc agacttaccg cagcctgagt ttgttctttg ttgaccggac
     3121 ggaacggttt gtcgttctga tgagtcacct gtaacgccaa cgcttcacca gatggcgtgg
     3181 tcgtgctggc atcgacttcc cagaactctt ccggcacgaa cgctttaatt tcacgctcac
     3241 gctcgaccac caggcgaacc gccaccgact gcacacgacc ggcagacaga ccacgagcga
     3301 tctttttcca tagcagcggc gaaaccatat accccaccac gcggtccata aagcgacgcg
     3361 cctgctgggc attaacacga tcaatattca gctcacccgg tttgttaaat gcctggcgga
     3421 tcgcgttttt agtaatttcg ttaaacacca ctcggctata gcgcgcatca tcacccccaa
     3481 tcacttcccg caggtgccat gcaatggctt ccccttcgcg gtcaaggtcg gttgcgagat
     3541 agatgtggtc ggctttttca gccagttgtt tcagttcaga gacgaccttc tctttaccag
     3601 gcaacacttc atagtgcgcc tcccaattgt gccacgggtc aacccccata cggttgacga
     3661 gagcgccacg ttcatccttt ttaggctttt tagccgtctt ggtggaggta gagtcggcac
     3721 tctttttggc agctgagcca ctggtcggca aatcgcggat gtgaccgacg ctggatttca
     3781 ccacgtagtc actacccaga tacttgttga tcgttttggc ttttgccggg gactcaacga
     3841 tgacaagagc tttacccata ttcaccttac ctaatttgat tcttccagga atgcgtcgca
     3901 cgttgataaa ccttccactg gcaacgagtt accgatatgg actcgttctc tgcggatatc
     3961 aaccccttgt cgtgactctg ttcacgaaaa ttcaggtgac tgagttaaca ggtcttggcc
     4021 tacaggcgct atagcacgac ggaattttgg tcgaatgtca agcaaatcta ttgccagatt
     4081 gacgaaagcg tcacactgta cctgataaaa tccgatacgc aactttatta gcatgcaaaa
     4141 tccaaagttg ccagtaaggc gctcttgcca ccagcgtggt atatttgtct gcaagccatg
     4201 cctggcgtag actaatgtcc ttgtttaagg agtaaataat gaacaaagag acacaaccca
     4261 tcgaccgcga aacgctgttg aaagaggcca acaaaatcat tcgtgagcat gaggatacgc
     4321 tcgcagggat tgaagccacc ggcgtcacgc agcgtaatgg cgtgctggta tttatcggcg
     4381 actacttttt agatgaacaa ggattgccaa cagccaaaag caccgcggtg tttaatatgt
     4441 tcaaacacct ggcccatgtg ctttcagaaa aatatcatct ggttgattaa agcaaaacgc
     4501 gaggtcttag cctcgcgttt gtcttttaca tcaatggctt ttgaccccgc tgccaccagc
     4561 gtagcaacaa tcgatcggcg ctctctgccg cgctgccggt gaatcggtca atgagtcgtt
     4621 tacgctgcat atagcgtaca ttaaccactt cacggccttc catcaggcta agaataactt
     4681 catcactggt gttgatttca tcaaccaggc ctttctctac cgcctgttgt ccgtaccagt
     4741 gttcacccgt tgccacctgt tcaatatcca gagacggacg catacgcttc acaaaatctt
     4801 taaataactg atgcgtttcg ttcagctctt cgcggaattt ctcccgccct tcttcggtat
     4861 tttcacccag caaggtcagc gtgcgcttat actgcccggc ggtgtgcagt tcgatatcaa
     4921 tatctttgct tttcaggaag cggttaaagt tgggcatttg cgccaccacc ccaatggaac
     4981 ccacaatagc aaacggtgcg gaaacaattt tgtccgccac acaggccatc atgtaaccgc
     5041 cgctggcagc gactttgtct accgtaacag ttaaaggaat gtttttatca cgcagacgct
     5101 gcagctgcga agccgccaac ccgtaaccat gcaccatgcc accagggctt tccagacgga
     5161 gcacaacctg atcctgcggt ttgaatgctg cgagtaccgc cgttatctct tcacgtagcg
     5221 agttcacttc atgggcgtcc atgctgcctt taaaatccag cacccagacg cgaggtttac
     5281 tgtcagttgc cacctcgccc agtttggctt tcgcttttgc tgctttcgct tcttgcttgt
     5341 gctttttctt ctgcgctttg tgccactgtt tttgctgatg tgagtccatc agcgccgcgg
     5401 ccagttcttc tttcatctcc ttatactgtt cgctgagatt gttgacccgt aactcgccac
     5461 gctggcgttt attacgttga gcaacattga caataatggc ggcaatcgcc gcaatcgcta
     5521 gcacaacggt aacgattttc gccaaaaaca aaccatattc agacaacaat tccacgagtc
     5581 cccaccttgg ttaaacaacg ctttggctca cagtttacaa caggcatgtt aagccgtctc
     5641 gcagagatgt tattgcagcg aaagtatgac aaagtatcca ttttgcggtg ttatcgcccc
     5701 aggctgattg aaatgccctt ctgtttcagg cataaagccc caaagtcata aagtacactg
     5761 gcagcgcgac agacaacgtc gttgaggagt agccatgcat taccagccaa aacaagattt
     5821 actcaatgat cgcattatcc tggtgacggg agccagcgat gggattggtc gtgaagccgc
     5881 gatgacgtat gcacgctatg gtgcgacagt gattctgttg ggccgtaatg aagaaaaatt
     5941 acgtcaggta gccagccaca taaacgaaga aactgggcgt cagccacagt ggtttattct
     6001 cgatttgctg acctgcacgt ccgaaaattg ccaacaactg gcacagcgca ttgtcgttaa
     6061 ttatccgcgt ctggatggtg ttttgcataa tgccggattg ctcggcgatg tttgcccaat
     6121 gagcgaacaa aatccgcagg tctggcagga cgtcatgcag gtcaacgtta atgccacctt
     6181 tatgctcacc caggcactgc ttcctttatt actcaaatcg gacgccggtt cactggtctt
     6241 tacttcatca agcgttggac gtcagggacg agccaactgg ggtgcatatg cagcgtcgaa
     6301 atttgccacc gaagggatga tgcaggtact ggccgatgaa tatcagcagc gcctgcgcgt
     6361 caactgcatt aacccaggcg gtacgcgcac cgcaatgcgc gcaagcgcct tcccgaccga
     6421 agatccacag aaacttaaaa cacccgctga tatcatgccg ctctacctct ggctgatggg
     6481 cgatgacagc cgccgtaaaa ccggcatgac ctttgacgcc caaccgggcc gtaaaccagg
     6541 aatttcccaa tgagtgatga acgctaccaa cagcgtcagc agcgagtgaa agaaaaagta
     6601 gatgctcgtg tggcccaggc ccaggatgaa cgcggtatta tcatcgtctt taccggcaat
     6661 ggaaaaggca aaaccaccgc ggcatttggt acggcaacac gcgcagttgg tcacggaaaa
     6721 aaagtgggcg tcgtgcagtt tattaaaggc acctggccta atggcgaacg caatctgctg
     6781 gagccacatg gcgttgagtt tcaggtgatg gcaacgggct ttacctggga tacacaaaac
     6841 cgcgagtctg ataccgccgc ctgccgcgaa gtctggcaac atgcaaagcg gatgcttgct
     6901 gattcctcac tggatatggt tttgcttgat gaactgacgt atatggtggc gtatgactat
     6961 ttgccactgg aagaagtggt gcaggcgtta aatgaacgtc cacatcaaca gacggtgatt
     7021 atcacgggtc gaggctgtca tcgggatatt cttgaactgg cggatacggt aagtgagctg
     7081 cgccccatca aacatgcgtt tgatgccggt gttaaggcgc aaatagggat tgattattaa
     7141 tctgtaaaaa tgccggatat gacgtccggc atactttgat taaccgttat tacgcccgcc
     7201 agaacgacga ccgccgctca cctgactgtg acgtttcacc gcacgacgaa tctgattagc
     7261 cttcatgcga cgacggtctt tttctacagc gactttagag ctggtttctg gcggaagctc
     7321 caccagttcg cgcagatagt tagtctgggc gaggtccagc tccgtccagc caccgcgtgg
     7381 cagacctttt ggcagtggga tatcaccgta acgaacacgg atcaggcggc tcacctgcac
     7441 accaaccgct tcccacagac gacgaacttc acggttacgg ccttcggtca gagtcacgtt
     7501 gtaccactgg ttgatccctt cgccgccgct gaacttgatg gttttaaaag ctgccggacc
     7561 atcttccagt tgcacgccac gactcaaatc acgcagtttc gcgtcgtcaa cctgaccaaa
     7621 tacacgcacg gcgtattcac gttcaacttc acggcttgga tgcattaaac ggtttgccag
     7681 ttcaccatcg gtggtgaaca gcagcagacc acaggtgtta acgtccagac gacctacggc
     7741 aatccagcgt gcgccacgca gctttggcag acggtcaaac accgtcggac gcccttccgg
     7801 atcgttacgg gtacacaact caccttccgg tttgtaatag gccagaacgc gacaaatttg
     7861 ttcagcggac tcacgtaccg aaatcagatg accatcgata cgaattttca gcccaggggt
     7921 aacttcaacg cgatcgccga gtttagcaat tttgccatca acactcacac gaccggcttc
     7981 aataatagat tcgatttcac ggcgggagcc gtggccagcg cgggccagca ctttctgtaa
     8041 tttttcgctc atagagcttc cttttggtgt cgccttccca ggcgtctgat atgcttttgt
     8101 gcaacagtga gtcagaactt acatctgact cactgatagt aaagtctgtt tgaactcttc
     8161 aggccttaag taaacgactc gacgttttaa ctggcctggc tttgaggcgc aacatactac
     8221 actatcttta tggttaaagc ttgtttcgat taacatcagc ttaattacca gccaccaccg
     8281 cctccgccac ccgcgccgcc accagcacca ccaccggaat aacttcgccc caacaaactg
     8341 gttttggccc ccagactcag gtgcatctgt aatgaaaaca aaacgccact acgggcaatt
     8401 tccggggcgc taacagccgc tgtgagtgat ggggtgatac gtgctaccca ttctcttcca
     8461 aggtttgctg agactgccca acctagcaac gcgtaatcca gtttccgtgt ttcccctttc
     8521 cgccgacgcc cactgtgtgt tgctgcctct ttacgcgcaa gatagttaac gatagcttca
     8581 ccgtgggcat aacgttgctg cccagtttgt gtaaactttg gcagaaaaat ataccccatg
     8641 ccagtgagat aaccggtcag gaaaacagcg ctaaaataac ccgctggcat ataccagtaa
     8701 aagaatacat ctccgacatt gatgtagaga gaatagaagg ccacgcctga acaaatcagt
     8761 ggtaaacaat tcttccgtcc tcaaggatgc ggccatttcg atatgtgtcg ccctcttctc
     8821 cagtaaagag atcgatttcg ctaaatggat tacccgcgat atcagggaac ttgagtgaaa
     8881 atcgggtctt atagatttca aacggccagt ggttaccagt cacgttccag attaacagat
     8941 ctgaatctcc ctcacgcagg aaagcatttt taacctgata atgaatttga taatgatagt
     9001 cgccttgagg caagaaacgt tgtttatcgc ccacgacaat actcattaac ccgatatgcc
     9061 agtcatgatg ccagggttca ggaataccat cacgggtgac gccaacaata tgatagttca
     9121 gcagtgcagc gtccccatca ggctgcatcc agagtcgtgg taaatcacga taaaatccgt
     9181 gtttaatctg ctgatttcgc gcctgaaaat gaatattttc tttgatgtcg agcaccccat
     9241 ccggttgcag agtggcttca acatcaaaga gcgaaatccc ttcttcataa gaagggatct
     9301 ccgtgttttc tgcataactt agagatgata caaatagccc tgctatcaac agcaaaatgc
     9361 agcgaaactt ccctgccata gtcttccatc cgtggaacgc aatttgttat aagaaaggct
     9421 tcacatcacc tacgccttca cgcaccacga ccggcgtatc atcggtaaga tcaataaccg
     9481 ttgtcggttt ctgcccgaga tagccgccgt gaataatcaa atctacctgt ttttccagac
     9541 ggtctttaat ttcttcggga tcggattcgg taaattcgct gcctggcagc attagcgaag
     9601 tggaaagcat cggttcgccc agtgcttcaa gcaacgcctg cgcaatcggg ttagaaggca
     9661 cacgcatacc gatggttttg cgtttttcct gcaacagacg gcgtggcact tccttcgtcc
     9721 ccttcaggat gaaggtatag ttgcccggcg tgttgttttt cattaaacga aacgcaacgt
     9781 tatcaacaaa tgaataggtc gagagttcag aaagatcgcg acacatcagg gtaaagttgt
     9841 gaccgtccgg cagctggcga atacgacaaa tacgctccat cgcgttttta tcttcaattt
     9901 tacagccgag cgcatagccg gaatcagttg gataaacaat caccccgcct ttacgcacga
     9961 tctccaccgc ctggttgatc agacgttgct gtgggttatc aggatgaata taaaaaaact
    10021 ggctcataat tccctctctg tggtgttctg cggctgttcc catagctgcc agacgccttc
    10081 aacgcctgcg ggcagccaga gtttacgacc tagttcgatc cacgggcatg gctgatgaaa
    10141 atcagatcct tgtgatgccc ataaatgatg ctgacgcgca agggccgcca gctgggtacg
    10201 ttcattgggc gattgctggc actgcgcgac ttccatcgcg tcaccgtggt gttcggcaaa
    10261 atgcgcaacc aatcttttca gccatttagc actaagattg taccgcccag gatgagctag
    10321 caccgcctta ccgccagaat gatgaatgac atcaatagct tgttctattg tacaccactg
    10381 tggcggaacg tatccggttt tcccgcgcgc cagatatttt ttaaagacat ccgccattga
    10441 actggctttg ccgcacccca caaggaaacg cgcaaaatga ccgcgcgtca ccgcaccgcc
    10501 ctgcgccagt cgtcgcgcgc cttccagtgc ccctggtatt tgcgcttttt caagtctttc
    10561 ggcaatcagt tgcgcccgct gattgcgccg ttctgtctgc tgcgcgagga actcacacat
    10621 tagcggatga gtaatatcaa tattcagccc gacaatatga atttcatgat tttcccagac
    10681 cgtggaaatt tccacgccgg gaataagatt cagcgccagt cccgaacgtg aaatttcctc
    10741 tcttgctggc acgattgcag ctgtgttgtc atggtcggtg atcgccaggg taccgacgcg
    10801 catctcgact gcacggtgca ccaatacttc tggcgtcagg cagccatcgg aagctgtggt
    10861 atggctgtgc aggtcgtaaa tcactgcgta attcgtgtcg ctcaaggcgc actcccgttc
    10921 tggataatgt tttttgcgcc gacatcataa cagttctggc aaatattctg aaatcagctg
    10981 ttgacaatta atcatcgaac tagttaacta gtacgcaagt tcacgtaaaa agggtatcga
    11041 caatgaaagc aattttcgta ctgaaagctt ggtggcgcac ttcctgaaac gggcagtgta
    11101 ttcaccatgc gtaaagcaat cagataccca gcccgcctaa tgagcgggct tttttttgaa
    11161 caaaattaga gaataacaat gcaaacacaa aaaccgactc tcgaactgct aacctgcgaa
    11221 ggcgcttatc gcgacaaccc gactgcgctt tttcaccagt tgtgtgggaa tcgtccggca
    11281 acgctgctgc tggaatccgc agatatcgac agcaaagatg atttaaaaag cctgctactg
    11341 gtagacagtg cgctgcgcat tacagcatta ggtgacactg tcacaattca ggcactttcc
    11401 ggcaacggcg aagccctgct ggcactactg gataacgccc tgcctgcggg tgtggaaaat
    11461 gaacaatcac caaactgccg tgtgctgcgc ttcccccctg tcagtccact gctggatgaa
    11521 gacgcccgct tatgctccct ttcggttttt gacgctttcc gtttattgca gaatctgttg
    11581 aatgtaccga aggaagaacg agaagcaatg ttcttcggcg gcctgttctc ttatgacctt
    11641 gtggcgggat ttgaagattt accgcaactg tcagcggaaa ataactgccc tgatttctgt
    11701 ttttatctcg ctgaaacgct gatggtgatt gaccatcaga aaaaaaacac ccgcattcag
    11761 gccagcctgt ttgctccgaa tgaagaagaa aaacaacgtc tcactgctcg cctgaacgat
    11821 cttcgccagc agctgaccga agccgcgccg ccgctgccgg tggtttccgt gccgcatatg
    11881 cgttgtgaat gtaatcagag cgatgaagag ttcggtggcg tagtgcgttt gttgcaaaaa
    11941 gcgattcgcg ctggagaaat tttccaggtg gtgccatctc gccgtttctc tctgccctgc
    12001 ccgtcaccgc tggcggccta ttacgtgctg aaaaagagta atcccagccc gtacatgttt
    12061 tttatgcagg ataatgattt caccctgttt ggcgcgtcgc cggaaagttc gctcaagtat
    12121 gacgccacca gccgccagat tgagatctac ccgattgccg gaacacgtcc acgcggtcgt
    12181 cgtgccgatg gctcgctgga cagagacctc gacagccgca tcgaactgga aatgcgtacc
    12241 gatcataaag agctttctga acatctgatg ctggtggatc tcgcccgtaa tgatctggca
    12301 cgcatttgca cccccggcag cagctacgtc gccgatctta ccaaagttga ccgttactct
    12361 tacgtgatgc acctggtctc ccgcgtggtc ggtgagctgc gccacgatct cgacgccctg
    12421 cacgcttacc gcgcctgtat gaatatgggg acgttaagcg gtgcgccgaa agtacgcgcc
    12481 atgcagttaa ttgccgaggc ggaaggtcgt cgccgcggca gctacggcgg cgcggtaggt
    12541 tatttcaccg cgcatggcga tctcgacacc tgcattgtga tccgctcggc gctggtggaa
    12601 aacggtatcg ccaccgtgca agccggtgct ggcgtagtcc ttgattctat tccgcagtcg
    12661 gaagccgacg aaacccgtaa taaagcccgc gctgtactgc gcgctattgc caccgcgcat
    12721 catgcacagg agactttctg atggctgaca ttctgctgct cgataatatc gactctttta
    12781 cgtacaacct ggcagatcag ttgcgcagca atgggcataa tgtggtgatt taccgcaacc
    12841 atattccggc gcagacctta attgaacgcc tggcgacgat gagcaatccg atactgatgc
    12901 tttctcctgg ccccggtgtg ccgagcgaag ccggttgtat gccggaactc ctcacccgct
    12961 tgcgtggcaa gctgcccatt attggcattt gcctcggaca tcaggcaatt gtcgaagctt
    13021 acgggggcta tgtcggtcag gcgggcgaaa ttcttcacgg taaagcgtcg agcattgaac
    13081 atgacggtca ggcgatgttt gccggattaa caaacccgct gccggtggcg cgttatcact
    13141 cgctggttgg cagtaacatt ccggccggtt taaccatcaa cgcccatttt aatggcatgg
    13201 tgatggcagt acgtcacgat gcggatcgcg tttgtggatt ccagttccat ccggaatcca
    13261 ttctcaccac ccagggcgct cgcctgctgg aacaaacgct ggcctgggcg cagcagaaac
    13321 tagagccagc caacacgctg caaccgattc tggaaaaact gtatcaggcg cagacgctta
    13381 gccaacaaga aagccaccag ctgttttcag cggtggtgcg tggcgagctg aagccggaac
    13441 aactggcggc ggcgctggtg agcatgaaaa ttcgcggtga gcacccgaac gagatcgccg
    13501 gggcagcaac cgcgctactg gaaaacgcag cgccgttccc gcgcccagat tatctgtttg
    13561 ctgatatcgt cggtactggc ggtgacggca gcaacagtat caatatttct accgccagtg
    13621 cgtttgtcgc cgcggcctgt gggctgaaag tggcgaaaca cggcaaccgt agcgtctcca
    13681 gtaaatctgg ttcgtccgat ctgctggcgg cgttcggtat taatcttgat atgaacgccg
    13741 ataaatcgcg ccaggcgctg gatgagttag gtgtttgttt cctctttgcg ccgaagtatc
    13801 acaccggatt ccgccacgcg atgccggttc gccagcaact gaaaacccgc accctgttca
    13861 atgtgctggg gccattgatt aacccggcgc atccgccgct ggcgttaatt ggtgtttata
    13921 gtccggaact ggtgctgccg attgccgaaa ccttgcgcgt gctggggtat caacgcgcgg
    13981 cggtggtgca cagcggcggg atggatgaag tttcattaca cgcgccgaca atcgttgccg
    14041 agctgcatga cggcgaaatt aagagctatc aattgaccgc tgaagatttt ggcctgactc
    14101 cctaccacca ggagcaactg gcaggcggaa caccggaaga aaaccgtgac attttaacac
    14161 gcttgttaca aggtaaaggc gacgccgccc atgaagcagc cgtcgctgcg aacgtcgcca
    14221 tgttaatgcg cctgcatggc catgaagatc tgcaagccaa tgcgcaaacc gttcttgagg
    14281 tactgcgcag tggttccgct tacgacagag ttaccgcgct ggcggcacga gggtaaatga
    14341 tgcaaaccgt tttagcgaaa atcgtcgcag acaaggcgat ttgggtagaa acccgcaaac
    14401 agcagcaacc gctggccagt tttcagaatg aggttcagcc gagcacgcga catttttatg
    14461 atgcgctaca gggtgcacgc acggcgttta ttctggagtg caagaaagcg tcgccgtcaa
    14521 aaggcgtgat ccgtgatgat ttcgatccgg cacgcattgc ctccatttat aaacattacg
    14581 cttcggcaat ttcggtgctg actgatgaga aatattttca ggggagcttt gatttcctcc
    14641 ccatcgtcag ccaaatcgcc ccgcagccga ttttatgtaa agacttcatt atcgaccctt
    14701 accagatcta tctggcgcgc tattaccagg ccgatgcctg cttattaatg ctttcagtac
    14761 tggatgacga ccaatatcgc cagcttgcag ccgtcgctca cagtctggag atgggggtgc
    14821 tgaccgaagt cagtaatgaa gaggaactgg agcgcgccat tgcattgggg gcaaaggtcg
    14881 ttggcatcaa caaccgcgat ctgcgtgatt tgtcgattga tctcaaccgt acccgcgagc
    14941 ttgcgccgaa actggggcac aacgtgacgg taatcagcga atccggcatc aatacttacg
    15001 ctcaggtgcg cgagttaagc cacttcgcta acggttttct gattggttcg gcgttgatgg
    15061 cccatgacga tttgcacgcc gccgtgcgcc gggtgttgct gggtgagaat aaagtatgtg
    15121 gtctgacgcg tgggcaagat gctaaagcag cttatgacgc gggcgcgatt tacggtgggt
    15181 tgattttggt tgcgacatca ccgcgttgcg tcaacgttga acaggcgcag gaagtgatgg
    15241 ctgcggcacc gttgcagtat gttggcgtgt tccgcaatca cgatattgcc gatgtggtgg
    15301 acaaagctaa ggtgttatcg ctggcggcag tgcaactgca tggtaatgaa gatcagctgt
    15361 atatcgatac gctgcgtgaa gctctgccag cacacgttgc catctggaaa gcattaagcg
    15421 tcggtgaaac cctgcccgcc cgcgagcttc agcacgttga taaatatgtt ttagacaacg
    15481 gccagggtgg aagcgggcaa cgtttcgact ggtcactatt aaatggtcaa tcgcttggca
    15541 acgttctgct ggcggggggc ttaggcgcag ataactgcgt ggaagcggca caaaccggct
    15601 gcgccggact tgattttaat tctgctgtag agtcgcaacc gggcatcaaa gacgcacgtc
    15661 ttttggcctc ggttttccag acgctgcgcg catattaagg aaaggaacaa tgacaacatt
    15721 acttaacccc tattttggtg agtttggcgg catgtacgtg ccacaaatcc tgatgcctgc
    15781 tctgcgccag ctggaagaag cttttgtcag cgcgcaaaaa gatcctgaat ttcaggccca
    15841 gttcaacgac ctactgaaaa actatgccgg gcgtccaacc gcactgacca aatgccagaa
    15901 cattacggct ggcacgaaca ccacgctgta tctgaagcgc gaagatttgc tgcacggcgg
    15961 cgcgcataaa actaaccagg tgctcggtca ggctttactg gcgaagcgga tgggtaaaac
    16021 cgaaatcatc gccgaaaccg gtgccggtca gcatggcgtg gcgtcggccc ttgccagcgc
    16081 cctgctcggc ctgaaatgcc gtatttatat gggtgccaaa gacgttgaac gccagtcgcc
    16141 taacgttttt cgtatgcgct taatgggtgc ggaagtgatc ccggtgcata gcggttccgc
    16201 gacgctgaaa gatgcctgta acgaggcgct gcgcgactgg tccggtagtt acgaaaccgc
    16261 gcactatatg ctgggcaccg cagctggccc gcatccttat ccgaccattg tgcgtgagtt
    16321 tcagcggatg attggcgaag aaaccaaagc gcagattctg gaaagagaag gtcgcctgcc
    16381 ggatgccgtt atcgcctgtg ttggcggcgg ttcgaatgcc atcggcatgt ttgctgattt
    16441 catcaatgaa accaacgtcg gcctgattgg tgtggagcca ggtggtcacg gtatcgaaac
    16501 tggcgagcac ggcgcaccgc taaaacatgg tcgcgtgggt atctatttcg gtatgaaagc
    16561 gccgatgatg caaaccgaag acgggcagat tgaagaatct tactccatct ccgccggatt
    16621 ggatttcccg tctgtcggcc cacaacacgc gtatcttaac agcactggac gcgctgatta
    16681 cgtgtctatt accgatgatg aagcccttga agccttcaag acgctgtgcc tacacgaagg
    16741 gatcatcccg gcgctggaat cctcccacgc cctggcccat gcgctgaaaa tgatgcgcga
    16801 aaacccggaa aaagagcagc tactggtggt taacctttcc ggtcgcggcg ataaagacat
    16861 cttcaccgtt cacgatattt tgaaagcacg aggggaaatc tgatggaacg ctacgaatct
    16921 ctgtttgccc agttgaagga gcgcaaagaa ggcgcattcg ttcctttcgt cacgctcggg
    16981 gatccgggca ttgagcagtc attgaaaatt atcgatacgc taattgaagc cggtgctgac
    17041 gcgctggagt taggcatccc cttctccgac ccactggcag atggcccgac gattcaaaac
    17101 gccactctgc gcgcctttgc ggcaggtgtg actccggcac aatgttttga aatgctggca
    17161 ctgattcgcc agaaacaccc gaccattccc attggcctgt tgatgtatgc caatctggtg
    17221 tttaacaaag gcattgatga gttttatgcc cagtgcgaga aagtcggcgt cgattcggtg
    17281 ctggttgccg atgtgccaat tgaagagtcc gcgcccttcc gccaggccgc gttgcgccat
    17341 aatgtcgcac ctatcttcat ctgcccgcca aatgccgatg acgacctgct gcgccagata
    17401 gcctcttacg gtcgtggtta cacctatttg ctgtcacgag cgggcgtgac cggcgcagaa
    17461 aaccgcgccg cgttacccct caatcatctg gttgcgaagc tgaaagagta caacgctgca
    17521 cctccattgc aggggtttgg tatttccacc ccggatcagg taaaagcagc gattgatgca
    17581 ggagctgcgg gcgcgatttc tggttcagcc attgttaaaa tcatcgagca acatattaat
    17641 gagccagaga aaatgctggc ggcactgaaa gtttttgtac aaccgatgaa agtggcgacg
    17701 cgcagttaat cccccagccg ccagttccgc tggcggcatt ttaactttct ttaacgaagc
    17761 cggaaaaatc ctaaattcat ttaatattta tcttttt
//
LOCUS       DAZPBM010000080        17619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPBM010000080 DAZPBM010000000
VERSION     DAZPBM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.080"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHUZQ_002444"
     CDS             complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHUZQ_002444"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1026117.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1575..1682)
                     /locus_tag="ACHUZQ_002445"
     CDS             complement(1575..1682)
                     /locus_tag="ACHUZQ_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026118.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHUZQ_002446"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHUZQ_002446"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1026119.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHUZQ_002447"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHUZQ_002447"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1026120.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHUZQ_002448"
     CDS             2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHUZQ_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1026121.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHUZQ_002449"
     CDS             4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHUZQ_002449"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1026122.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5607..8408
                     /gene="sucA"
                     /locus_tag="ACHUZQ_002450"
     CDS             5607..8408
                     /gene="sucA"
                     /locus_tag="ACHUZQ_002450"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1026123.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8423..9640
                     /gene="odhB"
                     /locus_tag="ACHUZQ_002451"
     CDS             8423..9640
                     /gene="odhB"
                     /locus_tag="ACHUZQ_002451"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1026124.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9734..10900
                     /gene="sucC"
                     /locus_tag="ACHUZQ_002452"
     CDS             9734..10900
                     /gene="sucC"
                     /locus_tag="ACHUZQ_002452"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1026125.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     CELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10900..11769
                     /gene="sucD"
                     /locus_tag="ACHUZQ_002453"
     CDS             10900..11769
                     /gene="sucD"
                     /locus_tag="ACHUZQ_002453"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1026126.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHUZQ_002454"
     CDS             complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHUZQ_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1026127.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12764..14680
                     /gene="mngA"
                     /locus_tag="ACHUZQ_002455"
     CDS             12764..14680
                     /gene="mngA"
                     /locus_tag="ACHUZQ_002455"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1026128.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14698..17331
                     /gene="mngB"
                     /locus_tag="ACHUZQ_002456"
     CDS             14698..17331
                     /gene="mngB"
                     /locus_tag="ACHUZQ_002456"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR1026129.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4264 a         4374 c         4792 g         4189 t
ORIGIN      
        1 ttacaatttt ttgtgtttgg gcatataaaa attaacccgc catttgaacg gcgggttaaa
       61 atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt
      121 catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc
      181 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      241 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      301 tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      361 gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg
      421 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      481 catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt
      541 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      601 tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
      661 aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
      721 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
      781 taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt
      841 aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacggt
      901 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac
      961 cacacatgac tgccattgga tgcgagtcgc gacggaaagc atggaacaga cgggtaatct
     1021 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1081 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
     1141 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1201 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
     1261 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1321 caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
     1381 ttagcgcctt attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1441 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1501 ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt
     1561 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1621 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1681 attatatgct tttcctggca atgtttgtaa caactttgtt gaatgattgt caaattagat
     1741 gattaaaaat taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg
     1801 acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc
     1861 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
     1921 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
     1981 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
     2041 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
     2101 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
     2161 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct
     2221 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
     2281 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
     2341 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
     2401 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
     2461 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
     2521 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt
     2581 ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
     2641 accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg gcaggtgttg
     2701 accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat tgtcgttgca
     2761 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     2821 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     2881 aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc
     2941 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     3001 gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga
     3061 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc
     3121 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     3181 acttcggcgg cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc ggtcacgcac
     3241 tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
     3301 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc gcactgtgca
     3361 tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg actggcgggg
     3421 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca
     3481 tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg
     3541 gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
     3601 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     3661 gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg
     3721 gtccgtgggg gccacacgcg aaactgaaac tcgatcacct gggtaaagaa gttctcgaat
     3781 cccgtctgcc gggtatcctg gagctttccc gtacctttgc tcacgtcgat ccggtgaaag
     3841 agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg accaaagtga
     3901 ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt ggttgttccg gggctgtttg
     3961 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     4021 tgctggacct ggtggtcttt ggtcgtgcgg caggtctgca tctgcaagag tctatcgccg
     4081 aacagggcgc attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgtctga
     4141 accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag
     4201 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     4261 agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     4321 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
     4381 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact
     4441 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat
     4501 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     4561 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     4621 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
     4681 gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     4741 tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     4801 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     4861 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     4921 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     4981 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     5041 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     5101 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     5161 acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga
     5221 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catattaagt
     5281 cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat
     5341 caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt
     5401 aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact
     5461 gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacat agacaagagc
     5521 tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc
     5581 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     5641 cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     5701 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     5761 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     5821 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag
     5881 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc
     5941 tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg
     6001 aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaacatga
     6061 aactcggcga gctgctggaa gccctcaagc aaacctactg tggcccgatt ggtgccgagt
     6121 atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6181 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6241 gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6301 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6361 gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6421 gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca
     6481 cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6541 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg
     6601 gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa
     6661 tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca
     6721 tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     6781 ttggtttcac cacctctaat ccactggatg ctcgttctac accgtactgt actgatatcg
     6841 gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct
     6901 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     6961 tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc
     7021 tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg
     7081 agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7141 cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca
     7201 cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7261 agcgcctgca ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt gaaatgcagt
     7321 ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg
     7381 actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc
     7441 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7501 accagtctaa cggttccact tacacgccgt tgcaacacat ccataacggg cagggcgcgt
     7561 tccgcgtctg ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa tacggttatg
     7621 ccaccgcaga accacgcact ctgaccatct gggaagcgca gttcggtgac ttcgccaacg
     7681 gtgcgcaggt ggttatcgac cagttcatct cttctggcga acagaaatgg ggccggatgt
     7741 gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg
     7801 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtt tgcgttccgt
     7861 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     7921 cgctggtagt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     7981 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8041 agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacagc
     8101 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8161 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8221 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8281 tgattccgtt tggggcttct ctgcgttatg caggccgcac ggcctccgcc tctccggcgg
     8341 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8401 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8461 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8521 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8581 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     8641 ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga
     8701 gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc
     8761 gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa
     8821 aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc
     8881 cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc
     8941 acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct
     9001 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9061 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg
     9121 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9181 tgcttctatc gacggtgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt
     9241 ttccacgccg cgcggcctgg tgacgccggt actgcgtgat gtcgataccc tcggcatggc
     9301 agacatcgag aagaaaatca aagagctggc agttaaaggc cgtgacggca agctgacggt
     9361 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9421 gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9481 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9541 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9601 gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca
     9661 ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg
     9721 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg
     9781 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
     9841 aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
     9901 agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg
     9961 gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
    10021 tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg
    10081 tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt
    10141 ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat
    10201 gccgtatcag ggatgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
    10261 gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgttgat
    10321 cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg acggcaaact
    10381 gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
    10441 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
    10501 cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatcgt
    10561 taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg caaccaaaga
    10621 acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt
    10681 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
    10741 agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
    10801 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
    10861 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
    10921 aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca ctcagaacag
    10981 gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc
    11041 cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc
    11101 gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga agctattgat
    11161 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgact
    11221 gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt
    11281 atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa
    11341 gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat
    11401 tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg acccgatccc aggctctaac
    11461 tttatcgaca tcctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc
    11521 ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc
    11581 aagccagttg taggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac
    11641 gcgggtgcga tcattgctgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa
    11701 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    11761 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga
    11821 agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt gcttatcgcg
    11881 atgattttcg ctgcgctatc agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc
    11941 tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca agaatcggct
    12001 ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga ggaatgattt
    12061 cctgttcgct acgatcgata accatcttct tcacttcttc aataaagtga tatttcgaat
    12121 tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag gtttcttcca
    12181 gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca cgatcctgcg
    12241 cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca aatatcagga
    12301 tttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg gttaactgaa
    12361 aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga atgctttcga
    12421 ggatctgctg ctcgactagc tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa
    12481 actctgtttg tagcgctgat tcagtgggta acgcatcgcc aggtttaagc tcgccacgcg
    12541 caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt cccattttta
    12601 gtatctcatt aatacgaatt tagccattat gcccgataaa ttcatcctgt aaataataca
    12661 aatacaatac aaataatttc aatcaagtga aattgatcac atgatggtat tgttttatcg
    12721 ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaatc tgacgactct
    12781 gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccatcca
    12841 cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgga
    12901 agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt tggctgtgcc
    12961 gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc
    13021 gcttcagtgg gaaggcgttg atggcccgga agcagttgat ttagtggtgc tgctggcgat
    13081 tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga ccacgcgcct
    13141 tgcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg agttgctctc
    13201 ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat
    13261 cgtctgcgta acggcctgtc cggcgggtat tgctcacacc tatatggctg cggaatatct
    13321 ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg
    13381 cattgaaggg cgtttaacgg cggatcaact caatagtgca accgcctgta tttttgcggc
    13441 tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt
    13501 tgccgagccg attcgccatg cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag
    13561 cgatgagacg cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca aaacggagct
    13621 gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg cggggggcac
    13681 ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga
    13741 agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg gaattttgat
    13801 ggtaccggtg ctcgcggcct ataccgccta ttctctggca gataaaccgg cgttagcgcc
    13861 aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt
    13921 tggcggattg atagccggtt acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag
    13981 taaattcaat ggattcctga ctttttatct ctacccggtg ctcggtacgt tgggagcggg
    14041 cagtctgatg ctgtttgtgg tgggagaacc tgtcgcatgg atcaataact cgcttaccgc
    14101 ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat
    14161 gtgttccttt gaccttggag ggccagtgaa caaagccgct tatgcattct gcctgggcgc
    14221 aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc
    14281 atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga
    14341 gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat
    14401 ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg
    14461 cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgccggca ttttctcgct
    14521 ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc
    14581 ggcattggtg ggggctgcaa tctcgactgc aattctcctg atctggcggc gtcacgcggt
    14641 taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg
    14701 aaagcggtat ctcgcgttca catcaccccg catatgcatt gggatcggga gtggtatttc
    14761 accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg
    14821 gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat
    14881 tatttcgcgg tgaaaccgga aaacaaagac cgcgtgaaga aacaggtaga agccggtaag
    14941 ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc
    15001 gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata
    15061 ggttatttac cggattcctt tggcatgtcc gggcaactgc cgcatattta caatggattt
    15121 ggcattaccc gcactatgtt ctggcgcgga tgttcggagc gccacggtac tgataaaacc
    15181 gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc
    15241 tacgccatcg gtaagtactt acctgccgac gaaaacggat tacgtaaacg cctcgacagt
    15301 tattttgacg tgctggaaaa agcgtctgta accaaagaga ttttgctgcc gaatggtcat
    15361 gaccagatgc cattgcagca aaatatcttc gaagtgatgg ataagctacg tgagatctac
    15421 cctcaacgta agtttgtgat gagccgcttt gaagaggtat ttgagaagat cgaagcgcag
    15481 cgtgaaagtc tggccaccct gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat
    15541 cgcaccatcg gttccacgcg catggatatc aaaattgccc acgcacgtat tgaaaataag
    15601 attgttaatc tgctggaacc gctggcaacg ctggcctgga cgttgggttt tgaataccac
    15661 cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagtatc
    15721 ggttgttgtt gcagtgacaa agttcatcgc gaaattgtcg cccgcttcga actggctgaa
    15781 gacatggcgg ataatctgct gcgtttctac atgcgcaaaa ttgccgataa catgccacag
    15841 agtgatgccg acaaactcgt gctgtttaac ctgatgccct ggccgcggga agaagttatt
    15901 aacaccactg tgcggctgcg cgccagccag tttaatctac aagacgatcg cggtcagcct
    15961 gtaccgtatt ttattcgcca tgcccgtgag atcgatcctg gcctaatcga tcggcaaatt
    16021 gttcattatg gcaattatga tccctttatg gagtttgata ttcagatcaa ccagattgtc
    16081 ccttctatgg gctatcgcac gctttatatt gaagcgaatc agcctggcaa cgtgattgcg
    16141 gcaaaaagtg acgctgaagg gattctggaa aatgctttct ggcagattgc gctcaatgag
    16201 gatggttctc tgcaactggt agataaagac agcggtgtgc gctatgaccg ggtattgcaa
    16261 attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg
    16321 gtaattaccg cagcgaacgc gaaaccgcaa tgcgatatta ttcatgaagc ctggcagagc
    16381 agggctgtta tccgctatga catggcagtg ccgctcaatt tgtcagaacg cagcgcccgg
    16441 caatccactg gcagagtagg gggggtgttg gttgtcactc ttagtcataa cagcaggcgt
    16501 attgatgtgg atatcaacct tgataaccag gctgacgatc atcgccttcg tgtcctggtc
    16561 cctacacctt ttaacactga cagtgttctg gcagatacgc agtttggttc gctaacgcgc
    16621 cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga agcgccggtt
    16681 ccggtatgga atatgctcaa ctatgttgcc ttacaggaag ggcgtaacgg catggctgtc
    16741 tttagcgaag ggttacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt
    16801 acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgcttttaag gcctgggcgg
    16861 ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct ttcttgtcgc
    16921 ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca gcaggcgcga
    16981 gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat gaagctcaac
    17041 aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc agtgggatgc
    17101 ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg gctgtttaat
    17161 ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt gatttcttgc
    17221 tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc aaatctatcg
    17281 gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg aatagcaatc
    17341 aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt tcatgccgga
    17401 tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata ttgcagagat
    17461 gatgtagaca ctggcaagcg tagcgcatca ggtaattttg cgtttatctt cactctcaag
    17521 ccacgtatat gtggctttat ttttaacaaa ataataaccc tgggtgagtt aattataata
    17581 taattacaag ttaattaaat gttaatatcg gcggggtgg
//
LOCUS       DAZPBM010000081        17381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPBM010000081 DAZPBM010000000
VERSION     DAZPBM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.081"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..501)
                     /locus_tag="ACHUZQ_002457"
     CDS             complement(<1..501)
                     /locus_tag="ACHUZQ_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1026130.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(687..771)
                     /locus_tag="ACHUZQ_002458"
     tRNA            complement(687..771)
                     /locus_tag="ACHUZQ_002458"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Leu,seq:caa)
     gene            967..1986
                     /gene="ahr"
                     /locus_tag="ACHUZQ_002459"
     CDS             967..1986
                     /gene="ahr"
                     /locus_tag="ACHUZQ_002459"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1026131.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHUZQ_002460"
     CDS             complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHUZQ_002460"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1026132.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2770..3801
                     /gene="idnD"
                     /locus_tag="ACHUZQ_002461"
     CDS             2770..3801
                     /gene="idnD"
                     /locus_tag="ACHUZQ_002461"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR1026133.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3825..4589
                     /gene="idnO"
                     /locus_tag="ACHUZQ_002462"
     CDS             3825..4589
                     /gene="idnO"
                     /locus_tag="ACHUZQ_002462"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR1026134.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4654..5973
                     /gene="idnT"
                     /locus_tag="ACHUZQ_002463"
     CDS             4654..5973
                     /gene="idnT"
                     /locus_tag="ACHUZQ_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR1026135.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6040..7038
                     /gene="idnR"
                     /locus_tag="ACHUZQ_002464"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6040..7038
                     /gene="idnR"
                     /locus_tag="ACHUZQ_002464"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR1026136.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7116..8618
                     /locus_tag="ACHUZQ_002465"
     CDS             7116..8618
                     /locus_tag="ACHUZQ_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1026137.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHUZQ_002466"
     CDS             complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHUZQ_002466"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1026138.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHUZQ_002467"
     CDS             complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHUZQ_002467"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1026139.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHUZQ_002468"
     CDS             complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHUZQ_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1026140.1"
                     /translation="MSIIILATISTTQENK"
     gene            11186..12697
                     /gene="pepA"
                     /locus_tag="ACHUZQ_002469"
     CDS             11186..12697
                     /gene="pepA"
                     /locus_tag="ACHUZQ_002469"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1026141.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12831..13274
                     /gene="holC"
                     /locus_tag="ACHUZQ_002470"
     CDS             12831..13274
                     /gene="holC"
                     /locus_tag="ACHUZQ_002470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1026142.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13274..16129
                     /gene="valS"
                     /locus_tag="ACHUZQ_002471"
     CDS             13274..16129
                     /gene="valS"
                     /locus_tag="ACHUZQ_002471"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1026143.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16185..>17381)
                     /locus_tag="ACHUZQ_002472"
     CDS             complement(16185..>17381)
                     /locus_tag="ACHUZQ_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1026144.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4455 a         4326 c         4447 g         4153 t
ORIGIN      
        1 gcgctgttga agacgagcag ccacttcaag acgtccagac atttctacgg ccttaatagg
       61 tgctaataaa tctcgagttt ttaactcagc gatattacgt tcaccaagcg ctgcaaaaag
      121 attatcttcc agacttttta gcacacgatg ggcatgatct tcagaccact ttttattggt
      181 gccatgccac tcaatcgcga cttctttaaa ggttcgtgct ttactctgtt caaccttatc
      241 acttttcttt ttgtctcccg gatcgacgcc attcgcaagc agcttacgtg gctcgtcacg
      301 acgtattctg gcatccgcga gtgtgatttc aggataaacc ccaagtgcca gcatcttttg
      361 ctttccctca taacggtact gcaaacgcca gtacttagaa ccatttggat ggacaagcag
      421 atgcataccg ccaccatcag tcaacttata ttgcttatct gttggcttag ctgttctgac
      481 tttgatgtct gttaatgcca tgcgtattcc ctccctgttg gtacaagcat tatcgaaccg
      541 gaaataccat catctgtacc aacatatgtt ggtagaagta cgttgatatc ggttgacctt
      601 gagagagtta atatagcggg aaaggtagat aaatactgaa tttcaggcat aaaaaagacc
      661 tcagttgagg tctattgaca tacttttggt gccgaaggcc ggactcgaac cggcacgtat
      721 ttctacggtt gattttgaat caactgcgtc taccgatttc gccacttcgg cactgaagag
      781 gtatgcggaa aacgttgtgg attatacctg tcgcacgcca ccatgcaagc gccagcttgc
      841 tcgcacctcg ctaagtgctg aaattttcag cattgcatac agcgatgtgt aacctttgtc
      901 acactccagg caccccgccc tgccatgctc tacacttccc agacaacacc agagaaggac
      961 caaaaaatgt cgatgataaa aagctatgcc gcaaaagaag cgggcggcga actggaagtt
     1021 tatgagtacg agcccggtga attgaagcca caagatgttg aagtgcaggt ggattactgc
     1081 gggatctgcc attccgatct gtcgatgatc gacaacgaat ggggattttc acagtatccg
     1141 ctggttgccg ggcatgaggt gattgggcgc gtggtggcgc tcgggagcgc cgcgcaggat
     1201 aaaggtttgc aggtcggtca gcgtgtcggg attggctgga cggcgcgtag ctgcgggcat
     1261 tgcgacgcct gtattagcgg taatcagatc aactgcgagc aaggtgcggt gccaacaatt
     1321 atgaatcgcg gcggttttgc cgagaagttg cgtgcagact ggcaatgggt gatcccactg
     1381 ccggaaaata tcgatatcga gtccgccggg ccgctgctgt gtggtggtat cacggtcttt
     1441 aaaccactgt tgatgcacca tatcactgct accagccgcg ttggggtaat tggtattggc
     1501 gggctggggc atatcgctat aaaacttctg cacgcaatgg gatgcgaggt gacagccttt
     1561 agttcgaatc ctgcgaaaga gcaggaagta ctggcgatgg gtgccgataa agtggtgaat
     1621 agccgcgatc cgcaggcact gaaaacactt gcggggcagt tcgatctcat tatcaacacc
     1681 gtcaacgtca gcctcgactg gcagccttat tttgaggcgc tgacgtatgg cggtaatttc
     1741 cacaccgtcg gtgcggtcct cacgccgctg tctgttccgg cctttacgtt aattgcgggc
     1801 gaccgcagcg tctctggttc agcaaccggc acaccttatg agcttcgtaa gctgatgcgc
     1861 tttgcggccc gcagcaaggt tgcgccgacc accgaactgt tcccgatgtc gaaaataaac
     1921 gacgccatcc agcatgtgcg caacggtaag gcgcgttacc gcgtggttct gaaagccgat
     1981 ttttgaaaat cattcgcagc gctgatctga ggcgctaccc cctttcgcac atattctgtc
     2041 ttgtcgtatc gccagcacgg cctgccgaca ttgttcggtg acattggcaa tatcatggtt
     2101 gatatcaatg cgtacaatat cctgttcatc tgcttgtgga cgctccagcg cctcaaactg
     2161 actttttagt aacgctaccg gcataaaatg cccagcccgc cgctgcattc gcgcgagaat
     2221 agtttcatag tcgccatcta accagaggaa atgaacatgg gggctaccct tgcgtaaaat
     2281 atcacgatac tgttttttta atgatgaaca gacaataaat cctgtttcat tctttttata
     2341 aagactgtat gaagcatcat ttaagcgttc cagccaggga agtcgatctt catcagataa
     2401 tggaataccc tgcgacattt tatctatatt tttggctgga tgaagatcgt caccatcaat
     2461 aaatttagca gataataacg cggcaacctt gctaccaatt aatgttttac cactccctga
     2521 aacgcccatc aaaataaagc tttcacccgc catttaaaat ctcctgaatc atcttacctg
     2581 ctgcgatact acgcctggtg gcataataat ttcacgtttt tttatttctg ctgataagaa
     2641 ttacaaggca catcacgtta tgcgtaacat agtaatgtaa caattttctg acgtgatctt
     2701 catcacaaat aatgacaatt aaaaccgctt aaatgcttcc gaggtgtcta cctgaccagt
     2761 gaggaattta tgcaagtgaa aacacagtcc tgcgttgttg cggacaagaa aactgttgcc
     2821 gttaccgagc agacgataga ttggaataat aatggaacat tagtacaaat aacccgaggt
     2881 ggaatttgcg gttccgattt acattattat caggaaggaa aagtaggtaa tttcatgata
     2941 aaggcaccga tggtgttagg tcatgaagtt atcggtaaag ttattcatag cgactcatca
     3001 gaattacatg aagggcaaac ggtagccatt aatccgtcta aaccgtgcgg tcactgcaaa
     3061 tactgcattg aacataacga gaatcagtgt acagatatgc gtttttttgg cagtgccatg
     3121 tatttccctc atgttgatgg tggttttacc cgttataaaa tggtcgaaac gtcgcaatgt
     3181 gtcccttatc cggccaaagc tgacgaaaag gttatggctt ttgccgaacc tttagccgtc
     3241 gcgattcatg ccgcacatca ggccgacgag ttacagggca agcgagtatt tgtttccggt
     3301 gttgggccca ttggctgcct gattgtcagt gctgtgaaaa cactggggtc cgcggaaatt
     3361 gtctgtgccg atgtgagtcc ccgttccctt tcgctggcca aagagatggg tgcggatgtg
     3421 ctcgtaaacc cacaaaacga cgacatggat cactggaaag cggaaaaagg ccatttcgat
     3481 gtcagctttg aagtgtccgg tcatccttca tcagtgaata cctgtctgga ggtcactcgt
     3541 gcacgcggcg taatggtgca ggtagggatg ggaggcgcga tggcagaatt cccaatgatg
     3601 acgttgattg gtaaggagat ttcactcaaa ggctctttcc gttttaccag cgaatttaat
     3661 accgcagtgt catggctggc gaatggcgtt atcaatccac tgcctttact gagtgctgaa
     3721 tatctcttca ctgacctgga agaggcgcta cgtttcgccg gtgataaaac ccaggcagca
     3781 aaagtccagc ttgttttcta agagataaat aaaggaataa tacaatgaac gatctatttt
     3841 cactggcagg aaaaaatatc ttgattaccg gttcagcaca gggcattggc tatttactgg
     3901 caaccggcct gggtaaatat ggcgcacaaa taattattaa tgatattact gccgaacgcg
     3961 cagaacttgc tgtagaaaaa ctccaccagg agggtattca ggccgttgcc gcacctttta
     4021 atgttactca taaacatgaa attgatgccg ccgttgaaca tatcgaaaag gacatcggcc
     4081 ccattgatgt gctggtgaat aacgccggta tccagcgccg tcatcctttt actgagttcc
     4141 ctgaacaaga gtggaatgat gtgatcgcag taaaccagac cgccgtgttc ctggtatcgc
     4201 aagcggtaac tcgtcacatg gttgaacgca aggcaggtaa agttattaat atttgctcga
     4261 tgcaaagcga actgggacgt gacaccatca ccccttatgc cgcatcgaaa ggggcggtaa
     4321 aaatgctcac ccgcggcatg tgcgtcgagc tggcgcgcca caatattcag gtcaacggta
     4381 ttgcgccggg ctatttcaaa acagaaatga ctaaagcact ggttgaggac gaagccttca
     4441 ccgcctggtt gtgcaaacgg acccccgccg cacgctgggg agatccgcag gaactgattg
     4501 gtgctgcggt gttcctttct tcaaaagcct ctgatttcgt aaacggccac ctgttgtttg
     4561 ttgatggcgg catgttagtg gctgtttaac agccaccacc ctacgtgcag cgcgaaagcg
     4621 ctgcttttct ggcactaata agagataaag actatgccat taatcattat tgcggcaggc
     4681 gtcgcgctgc ttcttatcct gatgatcggc tttaaagtta acggctttat tgccctcgtt
     4741 ctggtagctg ccgtcgtcgg atttgccgaa gggatggatg cacaggccgt cctgcactct
     4801 atacaaaatg gtatcggcag cacgctcggc gggctggcaa tgatcctcgg tttcggggcc
     4861 atgttaggca agctgatttc tgatacgggc gcggcacaac gtatcgccac tacgctgatt
     4921 gctacttttg gtaaaaaacg cgtgcaatgg gcgctagtga tcaccggtct ggttgtgggc
     4981 ctcgccatgt tttttgaagt gggttttgtc ctgctgttgc cgttggtatt taccatcgtg
     5041 gcatcatctg gattacccct gttgtatgtt ggcgtaccga tggtagcagc gctctctgta
     5101 acccactgtt ttctgccgcc acatccaggg cctactgcca tcgcgactat ctttgaggct
     5161 aatctcggaa cgactttact gtatggattt atcattacca ttccgacagt tattgtcgca
     5221 ggaccgctgt tttctaaact gctaactcgc tttgagaaag caccaccgga aggcttattt
     5281 aatcctcatc tgtttagcga agaggagatg ccctccttct ggaacagtat ttttgctgca
     5341 gtgatcccgg tcatcctgat ggctatcgcc gccgtttgtg aaattacgtt accgaaaact
     5401 aacaccgtgc gcctcttctt tgaatttgtc ggtaaccctg ccgttgcgct gtttattgcc
     5461 attgttattg cgattttcac actgggccga cgtaatggac gcaccatcga gcaaatcatg
     5521 gatatcattg gggattctat aggcgctatc gcgatgattg tgtttattat cgctggcggc
     5581 ggcgcgttta agcaggtatt agtagatagt ggtgtcgggc agtatatttc acacttaatg
     5641 accggaacta cgctttcgcc gttattgatg tgctggactg tcgcggcgct gttgcgtatc
     5701 gctctgggct ctgccaccgt cgcggccatt accaccgcgg gtgtggtgtt gccgattatc
     5761 aacgttaccc atgccgatcc cgctttaatg gtactggcaa ctggtgcggg cagcgtgatc
     5821 gcgtcacacg taaacgaccc tggcttctgg ctatttaaag ggtattttaa tctgacggtt
     5881 ggtgaaacgt tgcgtacctg gacagtgatg gaaaccctta tttctattat gggtttgctg
     5941 ggcgtgttag ccattaacgc cgtattgcac tgaaagaata aaatattgtg atgtagtttc
     6001 gggggaagat tgaagattcc cccgacgttg ggagaagtta tgcgcaatca cagaatttct
     6061 ttacaggata tcgctacgct ggctggcgta acaaaaatga ccgtgagtcg ttatatccgc
     6121 tcgccgaaaa aggtggcaaa ggaaacaggc gagcgcatcg ccaaaattat ggaagagatc
     6181 aattatatcc ctaatcgcgc tcccggcatg ttgttgaatg cacaaagtta taccctcggt
     6241 atcttgattc cgtcatttca aaatcaactc ttcgctgaca ttctcgcggg aatagaatcc
     6301 gtcacctctg tgcataatta tcagaccctt attgctaatt acaattacga tcgcgattcc
     6361 gaagaagagt cggtgattaa cctgctctct tataacattg acgggattat tctttcggaa
     6421 aaatatcaca ccatcaggac ggtgaaattt ctgcgttcag ccaccattcc ggtcgtggaa
     6481 ttgatggatg tacagggaga acggctggat atggaggtcg gttttgataa tcggcaggct
     6541 gcttttgaca tggtgtgtac catgctggat aagcgagtca ggcgcaaaat tctgtatctc
     6601 ggttccaaag atgacacccg cgatgaacag cgttatcagg ggtattgcga tgcgatgatg
     6661 ctgcataatc tttccccatt acgcatgaat ccacgcgcca tctcatccat tcatttagga
     6721 atgcagttaa tgcgtgatgc ccttagcgca aacccggatt tagacggtgt attttgcacc
     6781 aatgacgata ttgcgatggg tgcgcttctg ctgtgccgcg agcgaaacct cgccgtgcca
     6841 gaacaaattt ccattgcggg ttttcatggg ctggagattg gcaggcaaat gatcccaagc
     6901 ctcgccagcg taattactcc ccgttttgat attggtcgga tggccgccca aatgttgctg
     6961 agcaagatta agaataacga tcacaaccac aacactgttg acctcgggta tcagatttat
     7021 cacggtaaca cgctttaggt tattttctct tcattacaca tattctcttt gccggacgtt
     7081 gcctatactc caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc
     7141 gcacgccgga cacagaactg tttttactgc cgggaatggc taaccgtcac gggctgatta
     7201 ctggcgcaac ggggacgggt aaaactgtca cgttgcaaaa actggcagag tcattgtcgg
     7261 aaatcggtgt tccggtgttt atggccgatg tgaaaggcga tctgaccggc atcgcgcagg
     7321 caggaacggc gtcggaaaaa ctgctcgcaa gactgaaaaa tatcggcgtc aacgactggc
     7381 aaccgcatgc caatccggtg gtggtgtggg atatctttgg cgagaaaggc catccggtgc
     7441 gggcgacggt ttcggatctg gggccgctgt tgctggcacg actgttgaat ctcaacgatg
     7501 tgcaatctgg cgtgctgaat attatcttcc gcattgcgga cgatcaggga ttgttgctgc
     7561 tcgactttaa agatttgcgg gcgattaccc agtacatcgg cgataacgcc aaatccttcc
     7621 agaatcagta cggaaatatc agtagcgcat cggttggtgc catccagcgc ggattactgt
     7681 cgctggaaca gcaaggcgca gaacacttct ttggtgagcc gatgctggat atcaaagact
     7741 ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaaacttt
     7801 atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctttcagag ttatatgaac
     7861 aattgcctga agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaagcac
     7921 atctgctgtt taacgatgcg ccacaggtac tgctggataa gattgagcag gtgataaggc
     7981 ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtca gatatcccgg
     8041 ataacgtgct agggcagctc ggtaatcgcg ttcaacacgc tttgcgtgct tttacgccta
     8101 aagatcagaa agcagtgaag gcagcggcgc aaaccatgcg ggccaatccg gcgtttgata
     8161 ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt ctcgatgcga
     8221 aaggaagtcc ttcagtggtg gaacgggcga tggtgatcgc accttgttcg cgaatggggc
     8281 cggtgacgga agatgagcgt aatggcctga ttaatcactc cccggtgtat ggcaaatatg
     8341 aagatgaggt ggaccgcgag tccgcctatg agatgctgca aaaaggcttt caggccagta
     8401 ccgagcagca aaataatccc cccgtgaaag ggaaagaggt ggcggtggat gacggtattc
     8461 ttggtggatt gaaggatatt ttgtttggca ctaccggacc acgcggcggg aagaaagatg
     8521 gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt gacgaatcag attgtacgcg
     8581 ggatgttggg gagtttgctg ggggggagaa aaaggtaagt cagacgtgat ctgtgcccct
     8641 caccctaacc ctctccccaa aggggcgagg ggaccgttcg aactcgtatt tgactttgtc
     8701 aacaataata aagggagctt tcgctccctt gttagattac gattttctca ttaacagcca
     8761 caggctgatt aagaagaagc tggcgcttgg caacagtgcg ccgatgatcg gcgggatgcc
     8821 ataaaccaac gtcagcgggc cgaagatctg gtccagtacg tagaagacaa aaccgaagct
     8881 gataccggtg accacacgca cgcccatcgg tacgctacgc agtgggccaa agatgaacga
     8941 cagcgccatc aacatcatca ccgccacgga tagcggctgg aagattttgc tccacatgtt
     9001 gagctgataa cgtccggcat cctgaccgct cgacttcaga tacttcacat agttgtgcaa
     9061 accactgatg gagagtgcat ccgggtccag cgccaccacg cccagtttgt ctggcgtgag
     9121 gttggttttc caggtgccgc tcaccgtctg cgaaccggtg atctgtttcg gattggtcag
     9181 atcagattca tcaacctgcg acagacgcca gactttatgt tccgggtcaa acttcgcggt
     9241 agcggcataa cgtacggatt gcagacgacg attctcgtta aaggcataaa tactgatgcc
     9301 acctaactct tcgtcacctt taacccgctc aatgtagacg aagttgttgc catctttcgc
     9361 ccataagcct tgctgagtgg agagcaacga gccgccgtac atcgcctgcg cacggtagtt
     9421 acgcgccatc tgctcgccct gcgacgcgac ccattcacca atcgccatcg tcagcaagac
     9481 cagcggaatg gcggttttca tcaccgacag cgccacctgc atacgggtaa aaccagaagc
     9541 ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca acgccccaag
     9601 cagagccgcc atcgggaaga agatttgcac atctttcggc acgctcagca aggtatacat
     9661 tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcagctgat cgacaaactt
     9721 gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg tgaaaatagt
     9781 tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc tttacgcgaa
     9841 aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac aatcgccaaa
     9901 gccagataaa tcaggttaac ggtccacatc cacagcgtcg ggtccagctt acctttaccg
     9961 ccgttcgatt tcagggaggt ctggatcagg aagaaaagta gatacagcag catggctggc
    10021 agcatcgaca gtacgcgtcc ctgacgcggg ttaaccacgc tcaacggtac aaccataagt
    10081 gccatcataa acacggtgaa taccaacgtg atacgccagt tcagttctgc gcgggcacga
    10141 tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt cgggtcgagc
    10201 accaccgcct ggtgaccaat gatcgcctga taatcctgaa agtccgtaat gcggaaatcg
    10261 cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac ctgggagccg
    10321 tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg tgcattacct
    10381 tttggtcgaa tttgtgcgag gaacacatct ttgaaatcgc tgccgtcaac gctttcgatg
    10441 aacagcaccg agctgccatt agtcgcttgc tggaattgcc cttgcgccag cgccgccatg
    10501 ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga ccatggtccc
    10561 gcccacatca cgttaaccgc cgcgacgatt gccgtgaaca ccgcaaggat cattgccgct
    10621 ttcaccagca ccgctttgct cagaccgcag gcatgcatta ccgtaatttc actttcggta
    10681 tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag gataagctgc
    10741 gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg aatatcgccg
    10801 tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa aagcaagatg
    10861 aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat gattatcact
    10921 ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca tggcttaaac
    10981 gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc aacggattca
    11041 cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta acacgaagtc
    11101 atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc accgccgttg
    11161 tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta gcccggagaa
    11221 acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt ctccgattgc
    11281 agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgtc ggggcgaact
    11341 ggaaggaaaa ccggggcaga cactgttgct gcaccatgtt ccgaatgtac tttccgagcg
    11401 aattctcctt attggttgcg gcaaagaacg tgagctggat gaacgtcagt acaagcaggt
    11461 tattcagaaa accattaata cgctgaatga tactggctca atggaagcgg tctgctttct
    11521 gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg ctgtcgagac
    11581 tgcaaaagag acgctctaca gtttcgatca gctgaaaacg aacaagagcg aaccgcgtcg
    11641 tccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt gaactgacca gcggtgagcg
    11701 cgcgatccag cacggtctgg cgattgccgc cgggattaaa gcagcaaaag atctcggcaa
    11761 tatgccgccg aatatctgta acgccgctta cctcgcttca caagcgcgcc agctggctga
    11821 cagctacagc aagaatgtta tcacccgcgt tatcggcgaa cagcagatga aagagctggg
    11881 gatgcactct tatctggcgg tcggtcaggg ttcgcaaaac gaatcgctga tgtcggtgat
    11941 tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg gtaaaggttt
    12001 aaccttcgac tccggcggta tctccatcaa gccttcagaa ggcatggatg agatgaagta
    12061 cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg atggtcgcag agctacaact
    12121 gcctattaac gttattggcg tgctggcagg ctgtgaaaac atgcctggcg ggcgtgccta
    12181 tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc tgaacaccga
    12241 tgccgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgaacgtt ttgagccgga
    12301 agcggtgatt gatgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg gtcatcatat
    12361 tactggtctg atggcgaacc ataatccgct ggcccatgaa ctgattgccg cgtctgaaca
    12421 atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac aactggagtc
    12481 caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta ccgcaggttg
    12541 cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac ctggatattg caggaaccgc
    12601 ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc tggcacagtt
    12661 cctgttaaac cgcgctgggt ttaacggcga agagtaagtt tgcgttttac cctcacccta
    12721 accctctccc taagggagag ggaaccgatc gagcgcaata gaaattcatt gctcatcttt
    12781 cagggtgagg aatgcagcaa tatttaaatc caccacaaga agccccatat atgaaaaacg
    12841 caacgttcta ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac
    12901 tggtgtgtga aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc atcgcctgtg
    12961 aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct
    13021 ttgtcccgca taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct
    13081 ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg
    13141 cagattttgc cactgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga
    13201 aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg
    13261 caacctggaa ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga
    13321 gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg
    13381 catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca
    13441 aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctatggca
    13501 ggtcggtact gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc
    13561 agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga
    13621 atggaaagcg gagtccggcg gcaccattac ccgtcagatg cgccgtctcg gcaactctgt
    13681 cgactgggag cgtgaacgct tcacgatgga cgaaggcctg tccaatgcgg tgaaagaagt
    13741 tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aaacgcctgg taaactggga
    13801 tccgaaactg cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc
    13861 gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagacg gtaaagatta
    13921 tctggtggtt gcgactaccc gtccggaaac cctgttgggc gataccggcg tggccgttaa
    13981 cccggaagat ccgcgttaca aagacctgat tggcaaatat gtcattctac cgctggttaa
    14041 ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt
    14101 gaaaatcact ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc acgcgctgcc
    14161 gatgatcaac atcctgacct ttgacggcga tatccgtgaa agcgcccagg tgttcgatac
    14221 caaaggtaac gaatctgacg tttattccag cgaaatcccg gcagagttcc agaaactcga
    14281 gcgttttgct gcacgtaaag cagtcgttgc tgcggttgac gcactcggcc tgctggaaga
    14341 aattaaaccg cacgacctga ccgttcctta cggcgaccgt ggcggcgtag ttatcgaacc
    14401 aatgctgacc gaccagtggt acgtgcgtgc cgatgtactg gcgaaaccgg cggttgaagc
    14461 ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg
    14521 gatgcgcgat attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc
    14581 tgcatggtac gacgaagcag gtaacgttta tgttggccgc aacgaagaag aagtgcgcaa
    14641 ggaaaataac ctcggtgccg acgttgccct gcgtcaggac gaagacgttc tcgacacctg
    14701 gttctcttct gcgctgtgga ccttctctac cctaggctgg ccggaaaaca ctgacgcact
    14761 gcgccagttc cacccaacca gcgtgatggt atccggcttc gacatcattt tcttctggat
    14821 tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt
    14881 gccgttccac accgtttaca tgaccggcct gattcgtgac gacgaaggcc agaagatgtc
    14941 caaatccaag ggtaacgtta ttgacccgct ggatatggtt gacggtattt cgctgccgga
    15001 actgctggag aaacgtaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa
    15061 gcgcaccgag aagcagttcc cgaacggtat tgagccgcac ggcaccgatg ccctgcgctt
    15121 caccctggcg gcgctggcgt ctaccggtcg tgacatcaac tgggatatga agcgcctgga
    15181 aggttaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac
    15241 ggaaggtcag gattgcggct tcaacggtgg tgaaatgacg ctgtcgctgg cggaccgctg
    15301 gattctggca gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg
    15361 cttcgatatc gctgcaggca ttctgtatga gttcacctgg aaccagttct gtgactggta
    15421 tctggagctg actaagccgg taatgaacgg tggcaccgaa gccgaactgc gcggtactcg
    15481 ccatacgctg gtgaccgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt
    15541 catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat
    15601 catgctgcag ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga
    15661 caccgaatgg ctgaaacagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaatat
    15721 cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg
    15781 cgtaaatgaa aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca tcaccgtgct
    15841 gcctgccgat gacaaaggtc cggtttccgt tacgaagatc atcgacggcg cagagctgct
    15901 gatcccgatg gctggcctca tcaacaaaga agatgagctg gcgcgtctgg cgaaagaagt
    15961 ggcgaagatc gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt
    16021 cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag gctatgcgga
    16081 agcgaaagcg aaactgattg aacagcaggc tgttatcgcc gcgctgtaat caaaacacag
    16141 tgatgaaaac gaaggccgga gcatgctccg gcctttttta tctcttacag aaatggtaaa
    16201 gaaggcataa ttccccgtga aatactggct aagaatcctt tatctggttg ttcttcttta
    16261 ttgattaaag gaaggtcatc caaatcgccc agcaaatacg tattttttgc ctgccagtca
    16321 atcatccata ttttcagcag tggataagcc agaccgcccg taatcccgga tatcaccacc
    16381 aacgcacaca tgcgataaag cataccgtgg tatgttaaag ttgagcgaaa acgaatacgc
    16441 ccatcattca gtgacaggtt gctcataaaa tgatttcgca acgacaccgt taaataactg
    16501 gtgctaacag caatcccaaa ataatagatt aactgcgcga ttatcatttt acgttgcatt
    16561 tccataaatt gctgtaaatt ctcaatatca tcatttgcat acccagaact atcatactca
    16621 tttaatattt gatcgaagat aatataacca gcaacagcga gaaaaggtaa taaagccaaa
    16681 aatgcgagaa tggcatattt tatacaatat gtagtatcta atttcacctt gaaacgatgt
    16741 ataccgaaac tggtattact ccagagaaaa ctcattacca gactatataa agtgccatta
    16801 aaaataccaa tgaaaacaat acaaactatt gtcatcagaa ccacagatat aataacgcta
    16861 cttgaactat tggcatgtag catctttgtc gagataaaga aaacagtccc catcccaagg
    16921 gccattaaaa tcggcaagaa aaaggtcacc caccagaacc ctttcataga gcaattaaaa
    16981 ctaaatctta cgccgttgag actggtcatc aaggcctgat aacgcagtcc ttttgctgcc
    17041 ataaatataa gcaaaaccaa cagtgacaaa gtcaaaacac agccaataag cggcatatct
    17101 gctgacactg tcattaagat tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa
    17161 acattcccac cggtaatccc ataagaaaat cgttgtccgt taacttccat attagcatag
    17221 agataacgct tacatttcat taatgcccat ggtaaataga tacctagcgt tataatcgtt
    17281 aacaacacat tcaccacaca aataagaaaa tatctctcac ctgtaccatg aaaaacaaac
    17341 gaatgggacg gaacatccat atcattaata acttgagcca t
//
LOCUS       DAZPBM010000082        17140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPBM010000082 DAZPBM010000000
VERSION     DAZPBM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.082"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUZQ_002473"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHUZQ_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1026145.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHUZQ_002474"
     CDS             2219..2767
                     /locus_tag="ACHUZQ_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1026146.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUZQ_002475"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHUZQ_002475"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1026147.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUZQ_002476"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHUZQ_002476"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1026148.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUZQ_002477"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHUZQ_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1026149.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6727..8127)
                     /gene="asnS"
                     /locus_tag="ACHUZQ_002478"
     CDS             complement(6727..8127)
                     /gene="asnS"
                     /locus_tag="ACHUZQ_002478"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1026150.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACHUZQ_002479"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="ACHUZQ_002479"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1026151.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12377
                     /gene="pepN"
                     /locus_tag="ACHUZQ_002480"
     CDS             9765..12377
                     /gene="pepN"
                     /locus_tag="ACHUZQ_002480"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1026152.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12584..13351)
                     /gene="ssuB"
                     /locus_tag="ACHUZQ_002481"
     CDS             complement(12584..13351)
                     /gene="ssuB"
                     /locus_tag="ACHUZQ_002481"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1026153.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13348..14139)
                     /gene="ssuC"
                     /locus_tag="ACHUZQ_002482"
     CDS             complement(13348..14139)
                     /gene="ssuC"
                     /locus_tag="ACHUZQ_002482"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1026154.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14151..15298)
                     /gene="ssuD"
                     /locus_tag="ACHUZQ_002483"
                     /pseudo
     CDS             complement(14151..15298)
                     /gene="ssuD"
                     /locus_tag="ACHUZQ_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15295..16254)
                     /gene="ssuA"
                     /locus_tag="ACHUZQ_002484"
     CDS             complement(15295..16254)
                     /gene="ssuA"
                     /locus_tag="ACHUZQ_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1026155.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="ACHUZQ_002485"
     CDS             complement(16247..16822)
                     /gene="ssuE"
                     /locus_tag="ACHUZQ_002485"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1026156.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4295 a         4421 c         4349 g         4075 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcg tcaataagtt
     6181 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
     6241 catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt gctacaaaga taaagattta
     6301 gtttcaaaaa gaaaaaatat gtaaccaaaa gtaaaaatta aggaactttg tgaacaccgt
     6361 catatttcca tagaaacgtg atgatattta cagcaatttt tatctattta tatgatttac
     6421 ttatatttaa attaactaaa cggaaatttt gtttctgatg gaaactttat cgacctggca
     6481 caaaatcctc tttcagacat ccagaatgcc aaaaaatagt atgaaaattg tgctatcagc
     6541 taagaaaaaa ctaatccgca ataagaatgc gggttatttt ttgtgcgtaa atgttgcaat
     6601 gtaatattcc gtaacaggat gatcgttatg gcggggttgc aaatagattg cttgacttaa
     6661 caaacagata acttgacaga aaggataaaa aagagccagc aggcgctggc tctttgagga
     6721 agaaaatcag aagctggcgt tgcgcggagt acgtgggaac ggaatcacat cacgcacgtt
     6781 ttgcacgcca gttacgtaag caatcaggcg ttcaaaacca agaccgaaac ctgaatgcgg
     6841 aacagtaccg tagcgacgca gatcgcgata ccaccagtaa tcttctttat tcaggcccat
     6901 ttccagcata cgctcgtcca gaacgtccag acgttcttca cgctgggagc caccaatgat
     6961 ctcaccgatg cccggagcca gaacgtccat agccgcaacg gttttaccgt cttcgttgag
     7021 gcgcatatag aacgctttaa tatctttcgg atagttttta accactaccg gtgctttaaa
     7081 gtgttcttcc gccagataac gctcatgctc agaagagaga tcgacgcccc agtaaaccgg
     7141 gttttcaaac ttcctgccgc agttttcgag aatggtcact gcgtcggtgt aatccacctg
     7201 cgcgaaatcg gcctcaatga agcgttccag acgtgaaacg gcatctttat ctacgcgttc
     7261 agcgaagaat ttcatgtcgt ctgcgcgttc ttcgagaacc gctttgaaga catatttcag
     7321 catggcttca gccagacccg cgatatcgtt caggttagca aacgccactt ccggctccag
     7381 catccagaat tccgccaggt gacggctggt gttggagttt tcagcacgga aagtcgggcc
     7441 gaaggtataa attttggata atgcgcaagc gtaggtttcg ccgttcaact ggccagatac
     7501 ggtcaggaaa gactctttac caaagaagtc tttgtcgaaa tccactttgc cctgatcgtt
     7561 acgcggcagg ttttccagat ccagcgtaga aacgcggaac atttcgcctg caccttcggt
     7621 atcagatgcg gtaatcagtg gcgttgaaac ccagaagaat ccctgctcgt taaagaagcg
     7681 atgcagcgcc tgcgccagcg tatggcgaac gcgcgcgacg gcaccaatca ggtttgtgcg
     7741 cggacgcagg tgagcgactt cacgcagata ctcaatgctg tggcgttttg ccgccatcgg
     7801 gtaagtgtct ggatcttcaa cccaaccagc aacttcaacc ttgctggcct gaatttcaaa
     7861 ttgttgcccc tggcccggcg atgccacgac tttacccgtc acaatgaccg agcagccggt
     7921 ggtcagacgc aggacgtctt cattgtaatt gggcagagaa ttattgatga cagcctgtac
     7981 aggatcaaag caggaaccgt cataaacggc gaggaaggag atgccagctt ttgaatctcg
     8041 gcgggtacgt acccatccgc gtacggtgac ttcgctgtca acggctacgc ggccctggag
     8101 tacgtcggct acaggcacaa cgctcataat attctctctg ttaatagtcg gaaaaaataa
     8161 acacttgtcc acccgtaaat gggggggata cctatgttac ctggcatctg caatcagaca
     8221 agcagaattc gcaaatgcag cgaaagattt cggaattaaa ggtaaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccactt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaggg cgcactggtc atcagtaatt
    10021 tgccggagca ttttacgctt aagatcgtta atgaaataag cccggcggcg aataccgcgc
    10081 tggaagggct ttatcagtca ggcgatgcgc tttgcaccca gtgtgaagcc gaaggtttcc
    10141 gccatattac gtattatctc gaccgcccgg acgtgctggc gcgttttacc accaaaatta
    10201 ttgccgataa aaccaaatat cccttcctgc tttccaacgg taaccgcgtt gcgcaaggtg
    10261 aactggaaaa cggacgccat tgggtacagt ggcaggaccc gttcccgaaa ccgtgctacc
    10321 tgtttgcgct ggtggcaggc gactttgatg tactgcgcga cacctttacc acgcgttctg
    10381 gtcgcgaagt ggcgctggag ctgtacgtcg atcgcggcaa ccttgatcgc gcgccgtggg
    10441 cgatgacctc gctgaaaaac tcaatgaaat gggatgaaga acgcttcggc ctggagtatg
    10501 acctcgacat ctatatgatc gtcgcggtgg atttcttcaa tatgggcgca atggagaata
    10561 agggtctgaa tatctttaac tccaaatatg tgctggcccg caccgacacc gccaccgaca
    10621 aagattacct cgatattgaa cgcgttatcg gccatgaata tttccataac tggaccggta
    10681 accgagtgac ctgccgcgac tggttccagc tcagcctgaa agaaggttta accgtcttcc
    10741 gcgatcagga attcagctct gaccttggtt cccgcgcagt taaccgcatc aataatgtgc
    10801 gcaccatgcg cggattgcag tttgcggaag acgccagccc gatggcgcac ccaatccgcc
    10861 cggacatggt cattgagatg aacaacttct acaccctgac cgtttacgag aagggcgcgg
    10921 aagtgatccg tatgatccac accctgctgg gcgaagaaaa cttccagaaa gggatgcaac
    10981 tctatttcga gcgtcatgat ggtagcgcag cgacctgtga cgactttgtg caggcgatgg
    11041 aagatgcgtc gaatgttgat ctctcccatt tccgccgttg gtacagccag tccggtacgc
    11101 cgattgtgac cgtcaaagac gactacaatc cggaaactga gcagtacacc ctgaccatca
    11161 gccagcgcac gccagccacg ccggatcagg cagaaaaaca gccgctgcat attccgtttg
    11221 ccatcgaatt gtatgacaac gaaggcaaag tgatcccgtt gcagaaaggc ggtcatccgg
    11281 tgaattccgt gctgaacgtc acccaggcgg aacagacctt tgtttttgat aatgtctact
    11341 tccagccggt gcctgcgctg ctgtgcgaat tctctgcgcc agtgaaactg gaatataagt
    11401 ggagtgatca gcaactgacc ttcctgatgc gtcatgcgcg taatgatttc tcccgttggg
    11461 atgcggcgca aagtttgctg gcaacctaca tcaagctgaa cgtcgcccgt catcagcaag
    11521 ggcagccgct gtctctgccg gtacatgtgg ctgacgcttt ccgcgcggtg ctgctcgatg
    11581 agaagattga tccggcgctg gcggcagaaa tcctgacgct gccttctgtc aatgaaatgg
    11641 ctgaactgtt cgatatcatc gacccgattg ctattgccga agtacgcgaa gcactcactc
    11701 gtactctggc gactgaactg gcggatgagc tgctggctat ttacaacgcg aattaccaga
    11761 gcgagtaccg tgttgagcat gaagatattg caaaacgcac tctgcgaaat gcctgcctgc
    11821 gcttccttgc ttttggtgaa acgcatctgg ctgacgtgct ggtgagcaag caataccatg
    11881 aagcaaacaa tatgaccgat gcgctggcag cgctttctgc ggcggttgct gcacagctgc
    11941 cttgccgtga cgcgctgatg caggagtacg acgataagtg gcatcaggac ggtctggtga
    12001 tggataaatg gtttatcctg caagccacca gcccggcggc gaatgtgctg gagacggtgc
    12061 gtggtctgtt gcagcatcgc tcatttacca tgagcaaccc gaaccgtatt cgttcgttga
    12121 ttggtgcgtt tgcgggcagc aacccggcag cgttccatgc cgaagatggc agcggttacc
    12181 agttcctggt ggaaatgctt accgacctca acagccgtaa cccgcaggta gcttcacgtc
    12241 tgattgaacc gctgattcgc ctgaaacgtt atgatgccaa acgtcaggag aaaatgcgcg
    12301 cggcgctgga acagttgaaa gggctggaaa atctctctgg cgatctgtac gagaagatca
    12361 ccaaagcact ggcttgatac ttttgagcca aaatgcctga tgcgacgctt gacgcgtctt
    12421 atcaggccta caactccgtt gaaattgctg gttttgtagg ctggataagg cgttcacgcc
    12481 gcatccggca gccgtgccca actgcctgat gcgatgcttg acgtgtctta tcaggcctac
    12541 aagtccgttg aaaatgctgg ttttgtgcgc cacatccggc atattaacca tgacgccgaa
    12601 tcagttgttc agaatgccca cgccgcatta cccgttgcaa tacttccgct tccagctccg
    12661 ctaatcgcac cgaccctaat cgacgcggac gggggatatc cactgtcaaa tccagaccaa
    12721 tttttccctc ttcaattaac aacacccggt cagccattgc taccgcttcg ctcacatcat
    12781 gcgtcaccaa cagtacggta aagccgtgct gctgccaaag tgacacaatc aaatcctgca
    12841 tctcgagtcg cgttaaggcg tccagtgccc cgagcggttc atcaagcaac aataaacccg
    12901 gtcgatgaat caacgctctt gccagcgcca cacgctgttt ctgcccgcca gaaagtgcgg
    12961 caggccattc cccggcgcga ttctccagtc ctactgcagc caacgcccga cgcgcggcat
    13021 cgcgccactg gcctttaagg cctaacccaa cgttatcaat caccgatttc cacggcagca
    13081 gacgcgcatc ctgaaacatc attcgcgtgt cttcctgaat ctcagccagc ggtgtggtgc
    13141 ccgctaacac atcgcctgcg gttggcgttt ccagacctgc cagcaggcgc agcagggtac
    13201 ttttgccacc accgctgcgg cccaccaccg ccacaaactg acctgccgga atatgtaaat
    13261 ccagttggtt caggacgata ttttccgcgt aatgtttgct tactgcattg agcaacaatg
    13321 gtgtgccctg gttcagacga gcagtattca taccgtggcc tccttcaaat gataagccgg
    13381 gttccagcgc aaccagaggc gctctaacag ctgcgcgctg acgtcagcaa gtttgccgag
    13441 cagggcgtaa agaataatag cgactaccac cacgtccgtt tgcaaaaact cccgcgcatt
    13501 catcgccaga taaccaatgc ctgaattggc agaaatggtt tcggcaacaa tcagcgtcag
    13561 ccacatcagg cccaacgcaa aacgcacgcc gaccataatt gagggcaggg caccaggcag
    13621 gatcacatgg ataaacagcg gtatgccgga taatccatag ctacgcgcca tttccaccag
    13681 accgcgatcg atattacgga tgccatgcca ggtgttgata taaatgggga atagcgtgcc
    13741 gagcgccacc agaaagattt ttgcggattc atcaatgcca aaccacaaaa tcaccagcgg
    13801 gatcagcgcc agatgcggta cgttgcgcaa catctgaatt gaggtatcca gcagccgttc
    13861 tccccagcgt gacagcccgc taatcagtcc cagaatcaag cccagcgatc cgccaattga
    13921 aaagccaatc agcgcccgcc aggaactgat cgccagatgc tgccacaatt cgccgctggc
    13981 ggagagcgtc cagaacgccg tcaccactcc ttccggtgaa ggcaaaatac gcgtcgacag
    14041 ccagccaacc gaggaggcca gttgccacac cgccacgatg cccaccggta aaaaccaggg
    14101 ggcaacgcgc aataaccact tcttcactgg cgttgccatc atcgtgttcc ttagctttgc
    14161 gcgactttac gggggataaa atcattcgcc accgcttcgc cttgcggatt cagcggctgc
    14221 ggctggggaa tttccggaat ggcgacatcc agatgtggga acagcaactc gccaacccga
    14281 tacgcttctt ccagatgcgg gtagcccgaa agcacaaagc tgtcgatgcc aagcgcggcg
    14341 tattcgttga ttcgcgcggc gaccgtagga ccatcgccca ccagcgccgt tccggcaccg
    14401 ccgcgcacta agccaacgcc cgcccataaa ttggggctga tctccagatt gtcgcgcttg
    14461 ccgttatgta gcgccgccat tcgctgttgt cctacggaat ccgtccgggc gaatgcggcc
    14521 tgtgctttgg cgatagtttc atcatcaaga tgcgagatta accgctcggc ggcctgccac
    14581 gcttcgtcgt tagtttcacg aacaatcaca tgcagacgaa taccgaaacg aattttgcgt
    14641 ccatgcgcgg cagctttcgc ccgcacttgt tcgatcttct cttttaccag ctctggcggt
    14701 tcgccccagg tgaggtagag atcaacttgt tctgccgcca gctcctgggc gacatctgac
    14761 gatccgccaa agtaaagcgg aggatacggc tgttgaatcg gcgggaagag cagttttgct
    14821 ccccgcacat gaatatgttt accgttgaaa tcgacgcttt cccccagcaa taagcgcccc
    14881 gccagacctg ggtaaattcc gccgaggctt cgtagcgctc gctatgatca aggaacacgc
    14941 cgtcgcctgc caactcttgt ggatcgctgc ctgtgaccag attaaacaac gcacgaccat
    15001 ttgagagacg gtcaagcgtg gcggcctggc gagcggcaac ggtaggtgag gttacgctgg
    15061 gacgcagggc gacaagaaac ttcagccgct gcgtcaccgg gatcatcgac gccgccacca
    15121 gccacgcatc ttcacacgat cgccccgttg ggatcagcac accggtatag cccagacgat
    15181 ccgccgcttg cgcaatttgt tgcagatagc cgtgatcaac cgggcgtgaa ccttcttccg
    15241 ttccaagata atgcccgtca ccatgggtcg gtaaaaacca gaacatattc agactcataa
    15301 ttgttttcct tccaggtgag tgggctgcca gatgcgctgg cgaatatcaa cttttttcga
    15361 caccagacgg ttttcataaa acagatctgc cgtttgctgc tgtaaggcgg caacttcagc
    15421 gctcaccggt ttgatggtgg tgggaggacg atgatccaga tacgaggcaa tcaccggtgc
    15481 cggtaagccc atcgtttttg ccagtaaagc gatgctttgc tcgcgctggc tgcgggttaa
    15541 cgcatcggcc tcactaaagg ttgccagtac gccctgaata aaagcgccgt ttttttctgc
    15601 ataggggcga gctgccagat aaaacgatcc agtttgattg agatcggtgc cgtctttcag
    15661 cacccgcacg ccgccctgta ataacgcagc ggagtagtag ggatcccaga tagcccaggc
    15721 gtcaacgtta ccttgctgga acgcggcgcg ggcatcagct ggcgtcagat aagtgggctg
    15781 gatgtcagta aacttaagtc cggcctgacg cagtgcacgc agtaaaaggt tgtgtgaact
    15841 ggaacctttc tgaaaggcaa ctttgtgacc tttaagatcg gctacggttt tgatcgggct
    15901 gttttctgcc accagaatca cttcggcttt gggcttcggt ggctcgacgc ccacatatat
    15961 caaatcagcc ccggcagcct gggcaaagat tggcggaata tccccggtac tgccgagatc
    16021 aatactgcca acgtttaacg cttccaacat ttgcggaccc gcggggaact ccacccagga
    16081 gatttttgat tgcggatagc gtttttccag taactggtgg ctttttgcca ataccatacc
    16141 aatactgcct ttctgatagc ctatacgtaa cgcttcaggc gaggattctg cagcaaccgc
    16201 aaaagtagag acgctaagca atcccgccag cgccagttta atgatgttac gcatggacat
    16261 tacctcgcag agacagaagg tcaggaacct gaacatcgcg gcggtgcaat gcctgccaga
    16321 aagtttctag cgcggtatca agacgggttt gcagatttgg cgtgaactgg ggtttgtgat
    16381 ggtaatcaat aacttgtgag tcatcggcaa acacgccgtg caggagctcc tgggctttca
    16441 tcgcacttaa aactggctta agggcataat cgaccgccag cagatgagcc accgtaccgc
    16501 ccgtcgccag cggtagcacc actttgcctt gcaaagcgcg ctctggcagc aggtcgagca
    16561 gggttttcag cgcgccggaa taggcggctt tatacacagg tgtagcgact atcagtccat
    16621 cagcctgttg cagctgttcg gtgaaggtct tgagtgccgg actatcgaaa cgagcgtaaa
    16681 gcagatcttc cggggcgaag ttttgcagat tccagtgata aacctctaca tccaggccat
    16741 ttagtttttc ccgcgcatat tccagcaagg agctggagcg agaaggaaag cgaggactac
    16801 ccgccagggt gatgacacgc atactctctc cttataacca attgttcttt ttttgttaac
    16861 attgataaca attcggtcag tctgtcggag agacaagaaa atgccaaata taaattttgt
    16921 gtatcttttt ctgaaaataa actaaagaaa gggctttata tatcacgcat atttatttgt
    16981 tggatagtca ttagatgttg aatcaatgaa ttactcgttc agttaatatt taaatattca
    17041 tgaaatctat aaattaaaga tttgtcactt attggattta gtatgcttga tttaaatggc
    17101 ggtcaattat tgttattatt aagtctaatg tcatttaaag
//
LOCUS       DAZPBM010000083        17080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPBM010000083 DAZPBM010000000
VERSION     DAZPBM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.083"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHUZQ_002486"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHUZQ_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1026157.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUZQ_002487"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUZQ_002487"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR1026158.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHUZQ_002488"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHUZQ_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUZQ_002489"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUZQ_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1026159.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUZQ_002490"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUZQ_002490"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1026160.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHUZQ_002491"
     CDS             complement(6490..6846)
                     /locus_tag="ACHUZQ_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1026161.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHUZQ_002492"
     CDS             complement(6850..7266)
                     /locus_tag="ACHUZQ_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1026162.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUZQ_002493"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUZQ_002493"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1026163.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8750
                     /locus_tag="ACHUZQ_002494"
                     /pseudo
     CDS             8492..8750
                     /locus_tag="ACHUZQ_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9254..10447)
                     /gene="tyrB"
                     /locus_tag="ACHUZQ_002495"
     CDS             complement(9254..10447)
                     /gene="tyrB"
                     /locus_tag="ACHUZQ_002495"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1026164.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10700..11779)
                     /gene="alr"
                     /locus_tag="ACHUZQ_002496"
     CDS             complement(10700..11779)
                     /gene="alr"
                     /locus_tag="ACHUZQ_002496"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1026165.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11832..13247)
                     /gene="dnaB"
                     /locus_tag="ACHUZQ_002497"
     CDS             complement(11832..13247)
                     /gene="dnaB"
                     /locus_tag="ACHUZQ_002497"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1026166.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13330..14313
                     /locus_tag="ACHUZQ_002498"
     CDS             13330..14313
                     /locus_tag="ACHUZQ_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1026167.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14479..14721)
                     /gene="pspG"
                     /locus_tag="ACHUZQ_002499"
     CDS             complement(14479..14721)
                     /gene="pspG"
                     /locus_tag="ACHUZQ_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1026168.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14855..15892)
                     /gene="dusA"
                     /locus_tag="ACHUZQ_002500"
     CDS             complement(14855..15892)
                     /gene="dusA"
                     /locus_tag="ACHUZQ_002500"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1026169.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16255..16962)
                     /locus_tag="ACHUZQ_002501"
     CDS             complement(16255..16962)
                     /locus_tag="ACHUZQ_002501"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1026170.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(16986..>17080)
                     /gene="yjbL"
                     /locus_tag="ACHUZQ_002502"
     CDS             complement(16986..>17080)
                     /gene="yjbL"
                     /locus_tag="ACHUZQ_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1026171.1"
                     /translation="RHRDIIIAKIDLFIQRITHNLWHARKKMCF"
BASE COUNT         4434 a         4279 c         4074 g         4293 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgccaaag atttttctta tgccaaagat ttttcttatg ctaaagattt
     8641 ctttttatac atagaaacac agttaaaaat tgcaaaagat ttttgtagac ctggagaaga
     8701 agtatcaagt tctattgcaa gtaaagtttt tcacgcattt atcgatttag ttaacaaaat
     8761 caggggtaag aaagacttta tgtatatttg cacgctttgc tgttttgccg aggaggttaa
     8821 aggtgattat tctcattaca ggaccttttt atttgatatt ggtaatcaat ataaggttaa
     8881 acttacacaa agcggaaaaa aagagttctc tttaacatta gaatttaacg atactataat
     8941 tgaatctcag aaagtcacag gcaataaggc aaagtatatt cttgaggata tagaaatatt
     9001 ctatcgtaac aaacccgata cttattatta aatttaataa tggttagatg tgcagaaatt
     9061 attactccac acgaaaaaat agggaaagga attccagata attgggataa acttaacaat
     9121 aaaagaactg acaacgtttt attatctctc acgtagaaca acggcatcaa aaaaatcatc
     9181 tacaaagaaa tagtgaaagc gacgacagtt taatttcact gcaggctggg taactccagc
     9241 ctgctttcct gcattacatc accgcagcaa acgcctttgc cacacgttgt acatttgccg
     9301 tatttaaccc ggcgacacac atgcgaccgc tggcgatgag atagacacca aattcttcac
     9361 gtagtcggtc aacctgagcg gcacttaaac cggtataact gaacatgccg cgctgattaa
     9421 gcagataatc gaaattgcgt tctggcatct ctgtgcttaa taccttcacc aattcctgac
     9481 gcattgccag aatgcgagta cgcatctctt ctacttccgc cagccagctg gctttcaatg
     9541 cctcgtcatt cagcactgca gccaccacct gcgcaccaaa attcggcggg ctggagtagt
     9601 tgcggcgaac tgttgctttc aattgcccca gtacgcggcc agcggcttcg gcatcttcac
     9661 acagaacaga aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc gagaacgaat
     9721 tgctcaccag agcgggtaat ccagcgctgg caatggcgcg aatagcgtag gcatcctctt
     9781 ccataccggc actaaatcct tgataggcaa tatcgaggaa tgggataagc tcgcgggctt
     9841 tgagaatttc aatcaccgca tcccactgat cattagtgag atcggcaccc gttgggttgt
     9901 ggcaacatgg atgcagcaac acaatactgc gggcaggtaa tgttttcagc gtcgccaaca
     9961 ggtcattaaa gcgcacgctg ttagtcgctt cgtcatacca ggggtaagta ctcacttcga
    10021 atccagcccc ggcgaatatt gctacgtggt tttcccaggt aggatcgctg acccagacgc
    10081 ctgattccgg gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc cctgagccgc
    10141 caagggtttg aatggttgct acgcgctgtt gttgcagtac cggatggtcc gcaccaaaca
    10201 gcagcggcgc aatggcatgg cgatagctgt taagcccttc catcggtaaa taaagcgaag
    10261 cgccatgggg ctgcgcattc aggcgcgctt ccgcctccgc cacggctttc agttgtggaa
    10321 taattccgtc ttcgttgtag tacagaccga tacttaaatt cactttgtcg ctgcgagggt
    10381 cttctttaaa acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg tcaacttttt
    10441 gaaacacgcg atggttctcc aggtttacgg gcaggtggtt aaaacacaat aaaccggaag
    10501 aaggcgaaga tcgagtggat gttcaggagc gaacggcaat tagcaacaga gtgagactca
    10561 tgacaaacga acatccgcca gaggcacgac ctttataaga gcatgaaaga acatcaactt
    10621 attgaatttt taggatttta ttggccggat aaggcattca cgccgcatcc ggcacagaca
    10681 atcaaatatt acagaacgat taatccacgt atttcatcgc gacccttgaa gtcaggcgcg
    10741 taataagttc gtaagcgctc acttttgtca tttcagcgat acgttctacg ggcaaacctt
    10801 cgccccataa aatgaccgga tccccggctt tgtcctgcgc ctgtggacct aagtctacgc
    10861 agatcatatc catcgccacg cgtccgacaa tcggtacttc gcgaccgttc accagcactg
    10921 gcgtaccgga tggagcggcg cgcggataac catcgccata gcccatcgcg actacgccaa
    10981 gacgggtatc acgttcgctt acccaggttc caccataacc aacaggctct ccggctttat
    11041 gctcacgcac ggcaatcagg ctggaggtta gtgacatcac tggctgacag ccaaaatcgg
    11101 caccgatgga gcgatcttcc agcggcgaga cgccataaag aatgatgccc gggcgcaccc
    11161 agtcaaaatg cgattgtggc cacagcagaa tgccacccga cgcggcaatg gaacgttgac
    11221 caggtttgcc ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt gcgccacatt
    11281 ttggttcatc cgcgcgcgca aaatggctga cgatattcac cggctgacga acgtttttgc
    11341 actgtgtcag gcgatgataa aacgcctcag cctgttccgg ccttacgccc agacggtgca
    11401 taccggtatc gagtttcatc cagacggtaa ccggctcgtc caggctagcc tcttccagcg
    11461 cagccagctg ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa atcgtcggca
    11521 gatctctggc atcaaaaaag ccttcgagta acagtacagg tttggtgatt ccccccgcac
    11581 gcagtcgcag agcttcttcg agacgggcta cgccaaaggc gtcagcatcg gggagcgttc
    11641 gcgcggtctc aagaagaccg tgaccataag cgttcgcttt caccaccgca accattttac
    11701 tggcaggggc cagttcacga agacgttgca ggttgtgtcg cagagcgcgg cggttaatca
    11761 caacagttgc cgcttgcatt tgtgttcctt gataagtgtt tgctttaatt acctaattca
    11821 taaaataatt attattcgtc gtcgtactgc ggccccgcat agttgtcgaa gcgcgaccat
    11881 tgaccgttaa aggtcaggcg taccgtcccg attgggccgt tacgttgttt accgataata
    11941 atttccgcga tgccttttaa atcactgttt tcgtgataca cctcatcacg atagataaac
    12001 ataatcaagt ccgcatcctg ctcgatagag ccagattcac gcaggtcgga gttgaccggg
    12061 cgtttgtcgg cacgttgttc cagagaacgg ttcaactggg acagcgccac caccggcacg
    12121 ttcagttctt tcgccagtgc tttcagcgag cgagagattt ctgcaatttc cagcgtacgg
    12181 ttatcggaaa gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat aagcccgatg
    12241 ccgccgtgtt cacgggcaat acggcgtgcg cgggaacgca cttccgttgg cgtcaggccg
    12301 gaggagtcat cgatatagat attgcgtttt tcgagcaaaa tacccatggt gccggaaatg
    12361 cgcgcccagt cttcatcatc gagctgcccg gtacggattt tagtctggtc aacgcgcgac
    12421 agcgacgcca gagaacgcat cataatctgt tctgaaggca tctccagcga gaagataagt
    12481 actggtttat cctgcaacat cgccgcgttt tcgacgaggt tcatcgcaaa tgttgtttta
    12541 cccatcgacg gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc agcggttttt
    12601 ttgttgagat cgtcataacc ggtgtttact ccggtaacgc catcgtgtgg ctgctgaaac
    12661 aactgctcaa tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt cggcccttcg
    12721 tctttgtttg cacgactttc ggcaatttta aagacgcggg attcagcaag gtccagcaga
    12781 tcttcgctgg tacgcccctg cggatcaaaa ccggcttcag caatctcatt cgcaaccgag
    12841 atcatctcac ggacaacggc acgttcacgc acgatgtcag cataagcact gatgttcgcc
    12901 gcacttggcg tattttttga cagctctgcc agataagcaa aaccaccgac gctatcgagt
    12961 tgcccctggc gttccagcga ttccgcaagg gtaatcagat cgataggact accgctttcc
    13021 tgcaaacgcg ccatttcagt aaagatatgg cggtgtgggc gggtgtaaaa atcgtctgcc
    13081 accacacgct cggctacatc gtcccagcgt tcgttatcca gcattaaacc gcccaacacc
    13141 gactgctccg cttcgatcga gtgcggaggc actttcagcc cggcaacttg tggatcgcgt
    13201 tcgcggggtt cagcctgctg tttgttgaag ggtttatttc ctgccatagt gaatggagtt
    13261 accgagataa agaatgggtc gaaactttac catatgaagc agaccctgac gatacgttct
    13321 ggaggacaca tggcaacacg aattgaattt cacaagcacg gtggcccgga agtacttcaa
    13381 gccgtagagt tcactcctgc cgatccggca gagaatgaaa tccaggtcga aaataaagcc
    13441 atcggcatca attttatcga cacgtatatc cgcagcggcc tttacccgcc gccatcacta
    13501 cccagcggat taggtaccga agcggcaggc atcgtgagta aagtcggcag tggtgtaaag
    13561 catattaagg caggcgatcg tgtagtctat gcgcagtcgg cgttaggcgc ttacagctct
    13621 gtgcataaca ttaatgcgga taaagcggcg attctgcctg cggcaatttc ttttgagcaa
    13681 gctgcggcat ccttcctgaa aggcttaacg gtttattatc tgctgcgcaa aacctatgaa
    13741 attaaacccg atgaacagtt cctgttccac gcagcggctg gcggcgttgg cttaattgcc
    13801 tgccagtggg caaaagccct gggcgcgaaa cttatcggca ccgtaggaac cgcgcaaaaa
    13861 gcgcagagcg cgctaaaagc gggcgcgtgg caggttatta actatcgtga agaggatctg
    13921 gtcgagcggt taaaagagat caccggcggt aagaaagtgc gcgtggtgta cgattccgtg
    13981 ggcagagaca cctgggaacg gtcgctggat tgcctgcaac gccgcggctt aatggtcagt
    14041 tttggcaact catcaggtgc ggttaccggt gtgaacttag gcattctcaa tcaaaaaggc
    14101 tcgttgtatg tgacacgccc ttccctgcaa ggctatatca ccacgcggga ggaattaacc
    14161 gaggccagta atgaactgtt ctctttgatt gccagcggtg tgattaaggt cgatgtcgcc
    14221 gagcagcaga aatatccgct gaaggatgcg cagcgtgcgc atgagattct ggaaagccgg
    14281 gcgacgcaag gttccagcct gttgattcca taaaagaaat agggcttcca cctgggaagc
    14341 cctttctttt tatagttcgg ctgtatgtag ggtacagcac gatgaatctg ttagaggcgc
    14401 aatagtgaca gatttgatta tcaattccta ttttgttcta aggataaaac cttaggttgt
    14461 gatcatccgc acaatccctt agtaacgcca gcggtcataa cgctgatatt tcggcacttt
    14521 tggtgcttta atcgccttaa taacccacac caccgcaatc gccagtagta accacggcag
    14581 caacttaatc atcaatgcca gcataccgcc gaggaacata atggccgtcg ccacaaccag
    14641 cgcggcgata atgcccagca acgaaacgcc ggtgaccatc agcatgacaa aaaagccaat
    14701 cacaaaaagt agttccagca tgatgctctc ccaaatatga aatctcttgc tggcattaca
    14761 agaatcatgc caaaaataat ctattgattt aacagcaaaa cgccccgcga cggtgcgcag
    14821 ggcgtggtga atttgactac tttttggtga aaagttaacg cttatccgcc accagtttga
    14881 gcgcgtgttc cagcacatta atgtctgcac ccgctttatg ggcattttca cttaaataac
    14941 gccgccactg ccgcgcgcca ggaataccct ggaacaagcc caacatatgc cgggtaatat
    15001 ggccgagata cgtcccctgg ctgagttcac gctcaatgta cggatacatg gcgcgcacta
    15061 ccgccaccgg atcggcatcg gtatccgagg aaccaaagat ctcccggtct accgccgcca
    15121 gaatacccgg attctgatac gcctcgcgcc cgaccatcac gccatccata tgttgcaggt
    15181 gtgctttggc ctcttccagc gacttgatac caccgttaat cgacattgtc agatgcggaa
    15241 agtcacgctt cagttgatac acacgcggat aatcgagcgg cgggatttca cggttttctt
    15301 tcgggcttaa ccccgaaagc caggctttac gtgcgtggat gataaacatc tcacactcgc
    15361 ctttgccgga aacggtgttg atgaaatcgc agagaaattc atagctgtcc tggtcatcga
    15421 tgccaatacg cgttttcacc gtcactggaa tcgacaccac atcgcgcatc gctttcacgc
    15481 agtcggcaac cagctgcgca ttacccatca gacacgcacc aaacatgccg ttctgcaccc
    15541 ggtcagacgg gcagccgaca ttcaggttga tctcatcata tccacgcgct tctgccagct
    15601 tcgcacactg tgccagcgcc gccggatcgc taccgccgag ttgcaacgct accggatgtt
    15661 cttcttcact gtacgccagg taatcacctt taccgtgaat aatcgcccct gtggtcacca
    15721 tttcggtata cagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg caatgtctgt
    15781 ccgtccagtc gagcattggt gcaacgctaa accgaccact ccagtgaacg tcagtttttt
    15841 caggcattgc gctggtttgg ttgatttttt gcatttcaga attaccgtgc attttcaaat
    15901 gtagagatta ttttatcgat atatcatggg gttatgttat tcagcatcac tattcaggag
    15961 gctcaatagc ggggtactat accataacaa caggaagcgc ctgtctcatt gcaaaagaaa
    16021 attgagatcc tctcaaggca tgaagctctc acgaagtgat ggaaataatc ttattagccg
    16081 ttaactttgt taaggccaat gataaacaat ccaggttcga cgataaataa aaaatcacac
    16141 attaaactct ggtgatatat ctccctgcta atagcattga tagagaaaaa agaacccaat
    16201 aaatattggg tccttttata taatgccttc catactatcg aagaacttca catattattt
    16261 ctccgattta accccgaaca aatcataaat taatttagaa gtgtctgtaa gtattttaat
    16321 ctcttccttt gaggttgggt caaacgactt cgcgaagcta attaattgtg gtgcagcatc
    16381 tcgcattttg cttaaaatat caggatcgag cgttccttca ttcaccagat gtgacatctt
    16441 atccagatag ccatcaaaat tcatttctcc cccatcagcc agacgcttca tctctcccag
    16501 gtacttcttc atatcacgtt gagataattt ttcaaactga gctttcaggt agctctcatt
    16561 attttcatta atatacactg tttctgataa atctgcgtag gcactagaat atgaaagtga
    16621 aattaacagc gaagcaaata attttaagct gttgtcatca tgttcctggc aggcattaac
    16681 aaacgttaaa aaaccagaac ctattgcttg aaaatgtata ttcgctaaag gttcattatc
    16741 cttagattcc tcataaaata gctgaaccgt ggagggaagg ccattcttag tttctacatc
    16801 aaaagtacat tgttcaatgg gagtcgcatc tttattatcc ccaggattac atatactatt
    16861 aatcgcgaaa tgaaaaatag cccggtcaac aactgatgca ataaagtcac gatcgtttaa
    16921 atcctcatca gtgcaatcgt caatatactt attgacccac attttgagat catttatttg
    16981 ctcaattaaa aacacatttt tttacgtgca tgccataaat tatgcgtgat tcgttgaata
    17041 aacaaatcta tttttgcaat aattatatcc cgatgacgca
//
LOCUS       DAZPBM010000084        17021 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPBM010000084 DAZPBM010000000
VERSION     DAZPBM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17021
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.084"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHUZQ_002503"
     CDS             <1..266
                     /locus_tag="ACHUZQ_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1026172.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHUZQ_002504"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHUZQ_002504"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1026173.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUZQ_002505"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUZQ_002505"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1026174.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHUZQ_002506"
     CDS             complement(1570..3270)
                     /locus_tag="ACHUZQ_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR1026175.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHUZQ_002507"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHUZQ_002507"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1026176.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHUZQ_002508"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHUZQ_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="ACHUZQ_002509"
     CDS             4828..6600
                     /locus_tag="ACHUZQ_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1026177.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVSEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHUZQ_002510"
     CDS             6593..8374
                     /locus_tag="ACHUZQ_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1026178.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUZQ_002511"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUZQ_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1026179.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUZQ_002512"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUZQ_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1026180.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUZQ_002513"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUZQ_002513"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1026181.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHUZQ_002514"
     CDS             11336..11908
                     /locus_tag="ACHUZQ_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1026182.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHUZQ_002515"
     CDS             complement(11939..12250)
                     /locus_tag="ACHUZQ_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1026183.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHUZQ_002516"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUZQ_002516"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHUZQ_002517"
     CDS             12629..12982
                     /locus_tag="ACHUZQ_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFR1026184.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     NVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUZQ_002518"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUZQ_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHUZQ_002519"
     CDS             complement(14736..15206)
                     /locus_tag="ACHUZQ_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1026185.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUZQ_002520"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUZQ_002520"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR1026186.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHUZQ_002521"
     CDS             complement(16044..16262)
                     /locus_tag="ACHUZQ_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1026187.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUZQ_002522"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUZQ_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1026188.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         4067 a         4122 c         4558 g         4274 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gtcggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc aatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat g
//
LOCUS       DAZPBM010000085        16913 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPBM010000085 DAZPBM010000000
VERSION     DAZPBM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.085"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            292..1344
                     /locus_tag="ACHUZQ_002523"
     CDS             292..1344
                     /locus_tag="ACHUZQ_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR1026189.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1660..2862)
                     /locus_tag="ACHUZQ_002524"
     CDS             complement(1660..2862)
                     /locus_tag="ACHUZQ_002524"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1026190.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3094..4593
                     /gene="pitA"
                     /locus_tag="ACHUZQ_002525"
     CDS             3094..4593
                     /gene="pitA"
                     /locus_tag="ACHUZQ_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1026191.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4837..5172)
                     /gene="uspB"
                     /locus_tag="ACHUZQ_002526"
     CDS             complement(4837..5172)
                     /gene="uspB"
                     /locus_tag="ACHUZQ_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1026192.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5563..5997
                     /gene="uspA"
                     /locus_tag="ACHUZQ_002527"
     CDS             5563..5997
                     /gene="uspA"
                     /locus_tag="ACHUZQ_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1026193.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6096..6149)
                     /locus_tag="ACHUZQ_002528"
     CDS             complement(6096..6149)
                     /locus_tag="ACHUZQ_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026194.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6315..7784
                     /gene="dtpB"
                     /locus_tag="ACHUZQ_002529"
     CDS             6315..7784
                     /gene="dtpB"
                     /locus_tag="ACHUZQ_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR1026195.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7833..8585)
                     /gene="rsmJ"
                     /locus_tag="ACHUZQ_002530"
     CDS             complement(7833..8585)
                     /gene="rsmJ"
                     /locus_tag="ACHUZQ_002530"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1026196.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8593..10635)
                     /gene="prlC"
                     /locus_tag="ACHUZQ_002531"
     CDS             complement(8593..10635)
                     /gene="prlC"
                     /locus_tag="ACHUZQ_002531"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1026197.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10838..11680
                     /gene="rlmJ"
                     /locus_tag="ACHUZQ_002532"
     CDS             10838..11680
                     /gene="rlmJ"
                     /locus_tag="ACHUZQ_002532"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1026198.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11752..13104
                     /gene="gorA"
                     /locus_tag="ACHUZQ_002533"
     CDS             11752..13104
                     /gene="gorA"
                     /locus_tag="ACHUZQ_002533"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1026199.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13158..13241)
                     /gene="dinQ"
                     /locus_tag="ACHUZQ_002534"
     CDS             complement(13158..13241)
                     /gene="dinQ"
                     /locus_tag="ACHUZQ_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1026200.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13269..13442
                     /locus_tag="ACHUZQ_002535"
     CDS             13269..13442
                     /locus_tag="ACHUZQ_002535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026201.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13981..14334
                     /gene="arsR"
                     /locus_tag="ACHUZQ_002536"
     CDS             13981..14334
                     /gene="arsR"
                     /locus_tag="ACHUZQ_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1026202.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14388..15677
                     /gene="arsB"
                     /locus_tag="ACHUZQ_002537"
     CDS             14388..15677
                     /gene="arsB"
                     /locus_tag="ACHUZQ_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1026203.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15690..16115
                     /gene="arsC"
                     /locus_tag="ACHUZQ_002538"
     CDS             15690..16115
                     /gene="arsC"
                     /locus_tag="ACHUZQ_002538"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1026204.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16249..16545)
                     /locus_tag="ACHUZQ_002539"
     CDS             complement(16249..16545)
                     /locus_tag="ACHUZQ_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1026205.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16652..>16913
                     /locus_tag="ACHUZQ_002540"
     CDS             16652..>16913
                     /locus_tag="ACHUZQ_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1026206.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         3900 a         4387 c         4287 g         4339 t
ORIGIN      
        1 tatgttcgat gattttaagc accgagacta tccggtcttt tactaaataa tggaaaacaa
       61 tccatccctg gaagataatt gtaaaaaaaa caacagaata agaattaatc ttaggataaa
      121 tatttattta tcatggcctt ttaaccggta aaaaatggca ctcggcacag taaaccccgc
      181 tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa caggctgaaa
      241 ttatcattaa tgcacatttc attattagca gggatacctg aggagtttaa agtgaacata
      301 tatatcgggt ggcttttcaa attaatccct ttgattatgg gattaatttg catcgcgtta
      361 ggtggctttg tgctggaaag ttcagggcaa agcgagtatt tcgtcgcggg tcatgtgctg
      421 atttctctgg cggccatatg cctggcatta ttcactaccg catttattat catttcgcag
      481 ctcacgcgcg gcgttaatac gttttacaat acattgttcc ccattattgg ctatgcgggg
      541 tcaattatca ccatgatatg gggttgggca ctgttagcag gcaatgatgt gatggcagac
      601 gagtttgtcg ccggccatgt tattttcggc gttggtatga ttgccgcctg tgtatcgacg
      661 gtggcagcgt catccggtca ctttctgctc attcccaaaa atgcagcggg gagcaagagc
      721 gatggaacgc cggtacaggc ttattcttca ttaatcggta attgcctcat tgccgttccc
      781 gttttactca ccctgctcgg tttcatttgg tctattacgc tgttacgtag tgctgacata
      841 actccgcatt atgtcgcggg tcacgtattg cttgggttaa ccgcaatctg tgcctgtcta
      901 attggccttg ttgccacaat tgtccatcaa acacgtaata cgttttcaac taaagaacac
      961 tggctgtggt gttattgggt tatttttctc ggctcaatca cggtactgca ggggatatac
     1021 gtcttagtca gttccgatgc aagcgcccga ctggctcccg gcattattct tatttgcctc
     1081 ggaatgatct gttacagcat attctcaaaa gtctggctac tggcactggt ctggagacgt
     1141 acctgttcgt tagccaacag aataccgatg atacccgtct tcacctgcct gttttgcctt
     1201 ttcctggcat catttcttgc ggaaatggcg cagaccgaca tgggatattt tattccttcg
     1261 cgagttctgg tcggtttggg agcggtatgc tttacgttgt tctcaatcgt ttcaatatta
     1321 gaagcgggtt ctgctaaaaa ataattgcaa cgtaccggat aaaaccagcg ttgaccattt
     1381 gcgtaacgct ggtttttctt aggcatcatg aaataacgca gcattaatgc atagtggtta
     1441 aggataaaaa aacaacaaag ttactgtctt tttcagcctg ttcatatttt ataaagatct
     1501 ggccactggg tgagttttca atcacctttc cttccacctt atattaagcc tggagggttt
     1561 cagttcacgg gctcattaga aaataatcac aagatagcca ctatcaaaat tagctcattt
     1621 ttaatgcggc tctaataatt ttcatcttta ggtaacaggt caggacgact tcgctgcaat
     1681 caaatcctgc gcacaagccc acgcactcga ccacgcccac tggaagttat agccccccag
     1741 ccagccggta acgtccatca cttcgccgat gaagtacagc ccaggcactt tgcgcgcttc
     1801 catcgtccgt gaagagagtt cgttggtgtc cacgccgccg agcgtcactt cggcagtgcg
     1861 atagccttca gtgccgttgg gttgtacgcg ccagtcggtc aatgtgctaa tcagtgcctg
     1921 ttggtcacgc acgttgagct gttttagcga aacattcggg atttgcccga gttgctgtaa
     1981 gcgttcaacc aaccgcttcg gtagatgaac cgccagtgtg tttttcaggc tctgattcgg
     2041 atgtgcgtta cgctgctcat tcaggaaggt ttcgagatcc acatccggta gcagattgat
     2101 actgacaaat tcacccggtt gccagtagct ggaaatctgc aacaccgccg gtccagacaa
     2161 gccgcgatgg gtgaagagta agttctcacg gaaaacgatg ccgttttcag cggtaatcac
     2221 ggaaggcacc gccacgcccg ccagcacctg taactcttcg agcaacggtt tatgcagagt
     2281 gaatggcacc agacccgcac gggtcggcag cacgttgagg ccaaattgtt cggcaatctt
     2341 ataaccaaac ggcgacgcgc ccagccccgg cattgacagc ccaccggtcg ccatgaccag
     2401 cttttcgcaa ccgacagtca tgccgttcag atcaagcgtg aagcctgttt catcctccgc
     2461 cacactcagc acttcgctac gcaatctgaa ggtcacattg cccttctcgc actcatccac
     2521 cagcatgtcg acaatctgct gcgcggagtc atcgcagaag agttgcccta acgttttctc
     2581 gtgccatgcg atgccgtgtt tattgaccag atcaatgaaa tcccactggg taaaacgtgc
     2641 gagtgcagac ttacaaaaat gcggattctg gctcagataa gcgcctggtt cgacataaag
     2701 gttggtaaag ttgcagcgcc caccgccaga cataaggatt ttgcgccctg gttttttacc
     2761 attatcgatc agcagaaccc ggcgtcctgc ctgacctgcc agcgcagaac agaacatacc
     2821 cgccgcacca gcgcctataa taatggcatc aaacctttcc acgttgcgct cctcttagaa
     2881 aaaacgggcg tgaattgtaa agattcctca gtggtcgcac cagcatcaat attactaaaa
     2941 ggaagtattt gcctgaatta tataagataa ttattttttg agtgaaatcc atacaggagg
     3001 caaatcaaaa aaagtctata tttcactttg cccgcgccgc gaaagtcact gataatgcgc
     3061 cgcgttcatg tcctcaaaat ggcgtaacgt cctatgctac atttgtttgc tggcctggat
     3121 ttgcataccg ggctgttatt attgcttgca ctggcttttg tgctgttcta cgaagccatc
     3181 aatggtttcc atgacacagc caacgccgtg gcaaccgtta tctatacccg cgcgatgcgt
     3241 tctcagctcg ccgtggttat ggcggcggtg ttcaactttt tgggtgtttt gctgggtggt
     3301 ctgagtgttg cctatgccat tgtgcatatg ctgccgacgg atctgctgct taatatggga
     3361 tcgtctcatg gccttgccat ggtgttctct atgttgctgg cggcgattat ctggaacctg
     3421 ggtacctggt actttggttt acctgcatcc agctctcata cgctgattgg cgcgatcatc
     3481 gggattggtt taaccaatgc gttgatgacc gggacgtcag tggtggatgc actcaatatc
     3541 ccgaaagtat taagtatttt cggttctctg atcgtttccc ctattgtcgg cctggtgttt
     3601 gctggcggtc tgattttctt gctgcgtcgc tactggagcg gcaccaagaa acgcgcccgt
     3661 atccacctga ccccagcgga gcgtgaaaag aaagacggca agaaaaagcc gccgttctgg
     3721 acacgtatcg cgctgatcct ttccgctatc ggcgtggcgt tttcgcacgg cgcgaacgat
     3781 ggtcagaaag gcattggtct ggttatgttg gtattgattg gcgtcgcgcc agcaggcttc
     3841 gtggtgaaca tgaatgccac tggctacgaa atcacccgta cccgtgatgc catcaacaac
     3901 gtcgaagctt actttgagca gcatcctgcg ctgctcaaac aggctaccgg tgctgatcag
     3961 ttagtaccgg ctccggaagc tggcgcaacg caacctgcgg agttccactg ccatccgtcg
     4021 aataccatta acgcgctcaa ccgcctgaaa ggtatgttga ccaccgatgt ggaaagctac
     4081 gacaagctgt cgcttgatca acgtagccag atgcgccgca ttatgctgtg cgtttctgac
     4141 actatcgaca aagtggtgaa gatgcctggc gtgagtgctg acgatcagcg cctgttgaag
     4201 aaactgaagt ccgacatgct tagcaccatc gagtatgcac cggtgtggat catcatggcg
     4261 gtcgcgctgg cgttaggtat cggtacgatg attggctggc gccgtgtggc aacgactatc
     4321 ggtgagaaaa tcggtaagaa aggcatgacc tacgctcagg ggatgtctgc ccagatgacg
     4381 gcggcagtgt ctatcggcct ggcgagttat accgggatgc cggtttccac tactcacgta
     4441 ctctcctctt ctgtcgcggg gacgatggtg gtagatggcg gcggcttaca gcgtaaaacc
     4501 gtaaccagta ttctgatggc ctgggtgttt acccttccgg ctgcggtact gctttccggc
     4561 gggctgtact ggctctcctt gcagttcctg taatcgtacg caccaaaacg agcgggtcag
     4621 ctggcccgct tcagattgtg acatagtgcg ctttgttcat gccggatgcg gcgtgaacgc
     4681 cttatccggc ctacaaaaac atgcaaattc aatagattgc agagattatg taggcctgat
     4741 aagcgtagcg catcaggcaa ttttgcgttt gtaatcagtc tcgagcgggt cagtttactg
     4801 gcccgctttt tttatgcgtt attattgctg aagtgcttaa tgccaaatca tcaatgcaat
     4861 caggctgacc accaccagac cacacaatgc gctggtcaga ataaactgcc gacgcacccg
     4921 ctcacagcgg cgaataaatt catcatcgtg atgatcgcga taacgttggg catagatata
     4981 ccaaacgaga cgcacctgtt tgttgggttg gccatgtgag gtaaaaaagc cccctccatc
     5041 aacatattga tagagcaatg gatcgcagtt acgcagtacc actaacaacg cgcgtagtga
     5101 tgagaaatag cgcgccatgt taacaatgca aacgacacat aaagcccaaa ataatgcgac
     5161 ggtgcttatc atacctcctc cccggcgacc tgcccgcgga gttccacccc ggggctaccg
     5221 ctcccgatac gctgccaatc agttaacacc aggtcctgga gaaaccgctt ttgtggtgac
     5281 caacatccga gcggctctat agatagtgta ggagatcagg ttgttttttt tccagaaggt
     5341 taaccactat caatatattc atgtcgaaaa tttgtttatc taacgggtaa gcaaggcgga
     5401 ttgacggatc atccgggtcg ctataaggta aggatggtct taacactgaa tctttacggc
     5461 tgggttagcc ccgcgcacgt agttcgcagg acgcgggtga cgtaacggca caagaaacgc
     5521 tagctggcca gtcatcgaca actttatgga aggagtaaca ctatggctta taaacacatt
     5581 ctcatcgcgg tcgatctctc cccggaaagc aaagttctgg tagagaaagc agtctctatg
     5641 gctcgcccct acaatgcgaa agtttctctg atccacgtag atgtaaacta ctctgaccta
     5701 tacaccgggc ttattgatgt gaatctgggt gatatgcaga aacgcatctc tgaagagaca
     5761 catcatgcgc tgaccgagct ttccactaat gcaggctacc caatcactga aaccctgagc
     5821 ggcagcggcg acctgggcca ggttctggtc gatgcaatca agaaatacga tatggatctg
     5881 gtggtttgtg gtcaccacca ggacttctgg agcaaactaa tgtcttccgc acgtcagctg
     5941 atcaacaccg ttcacgttga tatgctgatt gttccgctgc gcgacgaaga agaataatca
     6001 tccccccacg acgtgttcct gaacgcccgc atatgcgggc gttttgcttt ttggcgcgcc
     6061 ttgttacctg atgcagcgta aaccccttat ctggcctacg atctgcgtac gcaatcaaaa
     6121 tctcctgcca atacaacatt taacactatc atattttcca tcattagtgt gatcagctgg
     6181 ttattttctg ttgtaatagt gtattaatct attcaccgca tcaatattaa gaatctctga
     6241 cagatgtaaa ctttttcgcg cgttatccct tacgcgttca tacttttcag gatggtattg
     6301 gaaggttaat aaatatgaat acaacaacac ccatggggat gctgcagcaa cctcgcccat
     6361 ttttcatgat cttttttgtc gagttatggg agcgattcgg ctactacggc gtgcagggcg
     6421 tactggcggt tttcttcgtt aaacagcttg gattctcgca agagcaggct tttgtcactt
     6481 ttggtgcttt tgctgcgctg gtctatggcc tcatttccat tggcggctat gtcggcgacc
     6541 acctgctggg gaccaaacgc accattgttc tcggagcact tgtgctggcg attggctact
     6601 tcatgaccgg catgtcgcta cttaagcctg acctgatttt catcgccctg gggactatcg
     6661 ctgtcggtaa cggcctgttt aaagctaacc cagccagctt gctttcgaag tgctatccgc
     6721 cgaaagatcc gcggcttgat ggcgcattca ccctgttcta tatgtcgatc aatatcggct
     6781 cgttgatagc gttatcgctg gcccctgtga tcgctgatag atacggttat tcagtcacct
     6841 acaacctgtg cggggcgggg ttaattatcg cattactggt ttacatcgcc tgtcgtggaa
     6901 tggtgaaaga cattggttct gaacccgact tcaagccgat gagcttcagc aaactgttgt
     6961 acgtattact tggcagcgtg gtgatgatct tcgtatgtgc atggctgatg cacaacgtag
     7021 aagtcgccaa tctggtgctg attgttctct ccatcgtcgt caccatcatc ttctttcgtc
     7081 aggcattcaa gctggataaa accgggcgca ataaaatgtt tgtcgccttt gtcctgatgc
     7141 tcgaagcggt ggtgttttac attctctacg cccagatgcc aacgtcgctg aacttctttg
     7201 ccatcaacaa cgtgcatcat gaaattctcg gcttttccat aaacccggtc agcttccagg
     7261 cgcttaaccc gttctgggtg gtactcgcca gcccaatact ggcaggcatt tacacgcatc
     7321 tgggtaacaa aggcaaagac ctctcgatgc cgatgaaatt tactctcggc atgtttatgt
     7381 gctcactggg ctttttgacg gcggcagctg cgggaatgtg gtttgcggat gcacaagggc
     7441 tgacatcgcc atggtttatc gtgctggtgt atttattcca gagcctgggt gaattgttta
     7501 ttagcgccct tggcctggcg atgattgctg ccctggtgcc gcagcatttg atgggcttta
     7561 ttctcgggat gtggttcctg acgcaggctg ccgcattctt gctgggcggc tatgtggcaa
     7621 catttaccgc agtgccggac aacattaccg atccgcttga gacgttaccc gtctatacca
     7681 acgtgtttgg taagattggc ctggttacgc tgggcgttgc agtagtgatg ctgttgatgg
     7741 tgccgtggct gaaacgcatg attgcgacgc cggaaagcca ttaattattc ttgcagaaag
     7801 cagggtagcg ttatcgctac cctgctttac ttttacacgg gcgtacctgc atagatatca
     7861 aagcgatgcc ctttagtcac aaccgcgttt ggcgtggcga cattcgctag cggtggcgcg
     7921 tagtccgggc gctttactac cacgcgtttg gtcgccagca agcgtgcagg ctccagcaat
     7981 ccatcggcat caagatccgg ccccaccagc gactgaaaga cacgcatctc ttttttcacc
     8041 agcgcgcttt tctgcttatg cgggaacatc gggtcgagat agaccacctg cgggcgtggg
     8101 gtaatatcgg ttagcgccgt caggctggag gcgtgaatta actgcaaacg ctcctgcaac
     8161 cagccgccga tttccgcatc cgcataacca cgcgccaggc cgtcgtcgag cagcgcggca
     8221 acaactggat tacgctccag catccgcacg cggcaaccga ctgaagccag cacaaaggca
     8281 tcgcgcccca gtcctgcagt ggcatccacc acatccggca aataatcgcc tttaatgccc
     8341 accgctttcg ccaccgcctc accgcgacca ccgccgaatt tgcgtcggtg cgccatcgct
     8401 ccgccaacaa aatcaacaaa gatgccgcca agttttggct catcacgctt gcgcaattcc
     8461 agatgttccg gcgttaacac cagcgccatc aggttgtctt catcgtgctc cagcccccag
     8521 cgggccgcca gaacagataa ggcgccgtct ccggcgcctg tttcatcaat taagcagatt
     8581 ttcactgaat gatcagccct taatgccgta atgctccagc atcgcatcca gctgcggttc
     8641 acgaccacgg aagcgtttga acagatccat cggctcttct gaaccgccac ggctcagaat
     8701 gttgtcgagg aacgactgcc cggtttcacg gttgaaaatg ccctcttcct caaagcgcga
     8761 gaaagcatct gccgccagta cgtcagccca caggtagctg tagtaacctg ccgcataacc
     8821 accggcgaaa atatggctga aagcgtgcgg gaaacggccc caggacggag atggcaccac
     8881 ggcaaccagt ttcttgattt ctgccagagt ttcgaggatt tttgccccct gatccgggcg
     8941 gaactcggca tgaaggcgga aatcaaacag gccgaactcc agctgacgca gaataaacag
     9001 cgccgcctgg tagttcttcg ccgccagcat tttatccagc aactctttcg gcagcggttc
     9061 gccggtttca tagtgaccag agataaacgc cagcgcctcc ggctcccagc accagttttc
     9121 cataaactga ctcggcagtt cgaccgcatc ccacggcaca ccgctgatac cggaaacacc
     9181 agcggtttcg atgcgggtca gcatatggtg caggccgtga ccgaactcgt ggaacagggt
     9241 gatcacttcg tcgtgagtga acagcgccgg tttaccattt accgggcggt tgaagttaca
     9301 agtcaaatac gcgaccggtt tttgcagaga accatcagct ttacgcatct ggcctacgca
     9361 gtcatccatc cacgccccgc cgcgcttgtt ttcacgggca tacagatcga ggtagaagct
     9421 gccgcgcagt tcgttatttt cgtcatacag ttcgaagaaa cgtacatccg gatgccagac
     9481 atcaacctct ttacgctctt tagcggtgat gccgtaaata cgtttcacca cttcaaacag
     9541 gccgttaacc gctttgtttt ccgggaagta cggacgcagc tgctcgtcgc tgatgctgta
     9601 gaggtgctgt ttctgttttt cgctgtagta agcgatatcc cacggctgca actcatcgac
     9661 accaaattcg gctttagcga aggcacgcag ttgctccagc tctttttcgc cttgcggacg
     9721 cgcgcgtttt gccagatcgg ttaagaaatc cagcacctgc tgcgggtttt ctgccatttt
     9781 agtggcaagg gatttaaagg cgtagttttc aaagcccagc agttgcgcca gttcgtgacg
     9841 cagcgcgagg atctcttcca tcaccttgct gttatcccac ttaccggcgt tcgggccttg
     9901 atcggaggcg cgggtgctgt aagcgcgata catctcttcg cgcagagcct ggttgtcgca
     9961 gtaggtcatt accggcaggt agcttgggat atccagcgtc agcaggtagc cttccagctc
    10021 tttcgcttcg gcctgggctt ttgccgcagc cagcgcgctt tctggcatcc ccgccagccc
    10081 cgcttcgtcg gtaacgagtt tggtccagcc catcgtcgca tcgaggacgt tgttgctgta
    10141 ctggttgccc agttcagaaa gacgggtagc aatttcgccg taacgctgct gtttctcttt
    10201 cggcagacct atgccagaga gttcaaaatc acgcagggcg ttatcaaccg ctttcttctg
    10261 cgccgtgttc agcgtggcgt aatgatcgcc atcgcgcagg tcgcggtacg ctttatacag
    10321 cccttcatgt tgccctaccc aggtgctgta ttccgacagc agcggcaggg tttgctcgta
    10381 ggcttcacgc agttccgggc tatttttcac cgagttcagg tggctgaccg gggagaagat
    10441 acgccccaac acatcgtcca cttccgccaa cggctggcag agattttccc aggtgtacgg
    10501 tgccccttgc gctactacgc gcgccacatt ttcgcggcaa tcgttcagcg cctgagtcac
    10561 ggctggaacg acatgttccg ggagaatttt agaaaacgga ggcaattcaa agggagtcag
    10621 taacggattc gtcattcgcg cagtcctggt taaagaggtt aaggaagcgc tcaacaggcg
    10681 ctttacataa tgtgtgtagc atggggttaa gtgtagtgaa tttcaatgag aaacgttacg
    10741 ctttcgcggc ggcggcctct tttcggtata ctgtcctgat acgcttttgt gcgccccgaa
    10801 tacgggccga tttttactta ccggaacacc tttacccatg ctcagttatc gccacagctt
    10861 tcacgctggc aaccacgccg acgtccttaa acataccgtt cagagcctga tcatcgagtc
    10921 gctgaaagag aaagataaac cgtttctcta tctcgatacc cacgcagggg ccgggcgtta
    10981 tcagttaggc agcgaacatg ccgagcgtac cggcgaatat ctcgaaggta tcgcccgtat
    11041 ctggcagcag gacgatttgc ccgcagaact ggaggcgtac atcaatgtgg taaaacactt
    11101 caaccgtagc ggtcagttgc gttactaccc cgggtcgccg ttgattgctc gccagctact
    11161 gcgcgaacaa gacagcctgc aactgaccga actgcacccg agcgattacc cgttgctgcg
    11221 ttctgaattt cagaaagaca gccgtgcgcg cgtcgaaaaa gccgacggtt tccagcagct
    11281 taaggccaaa cttccgccgg tttcccgccg tggtttaatc cttatcgacc cgccgtatga
    11341 aatgaaaacc gactatcagg cggtggtcag cgggatagca gaaggttaca aacgtttcgc
    11401 cactggtact tacgcactgt ggtatccggt ggtgctgcgt cagcaaatta agcgcatgat
    11461 ccacgatctg gaagcgaccg gtattcgcaa aattctgcaa attgaactgg cggtactgcc
    11521 agacagcgat cgccgtggca tgaccgcttc cggcatgatt gtgattaacc cgccgtggaa
    11581 actggaacag cagatgaata acgtgctgcc gtggctgcac agcaaactgg ttccggcagg
    11641 caccgggcac gccaccgtaa gctggatcgt gccggagtaa ttgcagccat tgctggcacc
    11701 tattacgtct cgcgctacaa tcgcggtaat caacgataag gacactctgt catgactaaa
    11761 cactatgatt acatcgccat cggcggcggc agcggcggta tcgcctccat caaccgcgcg
    11821 gctatgtacg gccagaaatg cgcactgatt gaagccaaag agctgggcgg cacctgcgtg
    11881 aacgtcggct gcgtgccgaa aaaagtgatg tggcacgcgg cgcaaatccg tgaagcgatc
    11941 catatgtacg gccctgatta tggtttcgat accaccatca acaagttcaa ctgggaaacg
    12001 ttgatcgcca gccgtaccgc ctatatcgac cgtattcata cttcctatga aaacgtgctc
    12061 ggtaaaaata acgttgatgt aatcaaaggc tttgcccgct tcgttgatgc caaaacgctg
    12121 gaggtaaacg gcgaaaccat cacggccgat catattctga tcgccacagg cggtcgtccg
    12181 agccacccgg atattccggg cgtggaatac ggtattgatt ctgatggctt cttcgccctt
    12241 cctgctttgc cagagcgcgt ggcggttgtt ggcgcgggtt acatcgccgt tgagctggcg
    12301 ggcgtgatta acggcctcgg cgcgaaaacg catctgtttg tgcgtaaaca tgcgccgctg
    12361 cgcagcttcg acccgatgat ttccgaaacg ctggtcgaag tgatgaacgc cgaaggcccg
    12421 cagctgcaca ccaacgccat cccgaaagcg gtagtgaaaa atgccgatgg tagcctgacg
    12481 ctggagctgg aagatggtcg cagtgaaacg gtggattgcc tgatttgggc gattggtcgc
    12541 gagcctgcca atgacaacat caacctagaa gccgctggcg ttaaaactaa cgaaaaaggc
    12601 tatatcgtcg tcgataaata tcaaaacacc aatattgaag gtatttacgc ggtgggcgat
    12661 aacacgggtg cagtggaact gacaccggta gcagttgcag cgggtcgccg tctctctgaa
    12721 cgcctgttta ataacaagcc ggatgagcat ctggattaca gcaacattcc gaccgtggtc
    12781 ttcagccatc cgccgattgg tactgttggt ttaacggaac cgcaggcgcg cgagcagtac
    12841 ggcgacgatc aggtgaaagt gtataaatcc tctttcaccg cgatgtatac cgccgtcacc
    12901 actcaccgcc agccgtgccg catgaagctg gtgtgcgttg ggccagaaga gaagattgtc
    12961 ggtattcacg gcattggctt tggtatggac gaaatgttgc agggcttcgc ggtggcgctg
    13021 aagatgggcg caaccaaaaa agacttcgac aataccgtcg ccattcaccc aacggcggca
    13081 gaagagttcg tgaccatgcg ctaaatgtta aagggctaag agtagtgtgc tcttagccct
    13141 taattacgtt tccgctatca gttcagaagc tgaagcagaa agcggatcag ttccagcagc
    13201 gcaattaacg ctccaagaac gatgattgct ttatcaatca cccgttttct ccatgcgatg
    13261 gagtgagaat gcatccgctt actcatccac tgcctgtcac ggcgcatgtc tcattgttag
    13321 atgacgacta tctcactccg gccagagcat cagttaacgg caccacccgt acttctgacc
    13381 aggactttga aagcgtttat gcgcattacc agagtgaaaa tgcctcagag ctaactggat
    13441 aatcatacag tacattcagg ttataaaacc agcatgtcct tgcaatagtt tcagtatggt
    13501 attagcattg atgcgttaga tgatgactat ctcactccag ccagagccac caactcaggg
    13561 ctgaagagta aaaaaaccga cgcaaagtcg gtttttttac atccggattc ggacaaggct
    13621 taatatgacg atgacccagt gaaagtatat aaatcgtcat tgcgatatat accgaagtgc
    13681 cccctccgcc agctgaagaa atcgctaatt cttgcaatgt tagccactgg ctaatagtat
    13741 tgaaccgtta gatgtgggca ttctcactcc agccagagcc accaactcag gctgaagagt
    13801 aaaaaaccga cgcaaagtcg gtttttttac gtcctgattc aaaccgcctt tcaaatgaat
    13861 agccaactca aaattcacac ctattacctt cctctgcact tacacattcg ttaagtcata
    13921 tatgtttttg acttatccgc ttcgaagaga gacactacct gccacaatca ggagcgcaat
    13981 atgtcatttc tgttacccat ccaattgttc aaaattcttg ctgatgaaac ccgtctgggc
    14041 atcgttttac tgctcagcga gctgggagag ttatgcgtct gcgatctttg tactgccctc
    14101 gatcagtcgc agcccaagat ctcccgccac ctggcattgc ttcgtgaaag cgggctattg
    14161 ctggaccgca agcaaggtaa gtgggttcat taccgcttat caccgcatat tccagcatgg
    14221 gcggcgaaaa ttattgatga ggcctggcga tgtgaacagg aaaaggttca ggcgattgtc
    14281 cgcaacctgg ctcgacaaaa ctgttccggg gacagtaaga acatttgcag ttaaaaattt
    14341 agctaaacac atatgaattt tcagatgtgt tttatccggg aggcattatg ttactggcag
    14401 gcgctatctt tgtcctgacc atcgtattgg ttatctggca gccgaaaggt ttaggcatcg
    14461 gctggagtgc aacgctcggc gcagtactgg cgttagttac gggcgtggtt catccgggtg
    14521 atattccggt ggtgtggaat atcgtctgga acgcgacggc tgcgtttatc gccgtcatta
    14581 tcatcagcct gctgctggat gagtccggct tttttgaatg ggcggcgctg cacgtctcac
    14641 gctggggtaa tggtcgtggt cgcttgctgt ttacctggat tgtcctgctc ggtgctgccg
    14701 ttgccgccct gtttgccaat gatggcgcgg cgcttatttt gacgccgatt gtcatcgcca
    14761 tgctgctggc tttaggattc agtaaaggca cgacgctggc gttcgtgatg gcggccggat
    14821 tcattgccga taccgccagc ctgccgctta ttgtctccaa cctggtgaat atcgtttccg
    14881 ctgatttctt tggcctcggc tttcgcgaat acgcctcggt gatggtgccg gtggatatcg
    14941 ccgcgattgt tgccacgctg gtgatgctac atctctattt tcgcaaagat attccgcaga
    15001 actacgatat ggcgctgctg aaatctcccg cagaagcgat caaagatcct gctacgttca
    15061 aaactggctg ggttgtttta ctgcttctgc tggtgggatt tttcgtcctg gaaccgctcg
    15121 gcattccggt gagcgccatt gcagctgtgg gcgcgctgat attatttgtc gtcgctaaac
    15181 gcggtcatgc gattaatacg ggtaaagtcc tgcgcggtgc cccctggcag attgtcatct
    15241 tctcgctcgg catgtatctg gtggtttatg gcctgcgcaa tgccggatta acggaatatc
    15301 tttctggcgt actcaacgtg ctggcggata acggcctgtg ggccgcgacg ctcggcaccg
    15361 gattcctcac cgccttcctc tcttctatta tgaacaatat gccgacggta ctggttggcg
    15421 cgttgtccat tgatggcagc acggcatctg gcgttatcaa agaagcgatg gtttatgcca
    15481 atgtgattgg ctgcgatttg ggaccgaaaa ttaccccaat tggtagcctg gctacgctac
    15541 tctggctgca cgtactttcg cagaagaata tgactatcag ctggggatat tacttccgta
    15601 cagggattat catgaccctg cctgtgctgt ttgtgacgct ggctgcgctg gcgctacgtc
    15661 tctctttcac tttgtaatga gatactgata tgagcaacat taccatttat cacaacccgg
    15721 cctgcggcac gtcgcgtaat acgctggaga tgatccgcaa cagcggcaca gaaccgacca
    15781 ttatccatta tctggaaact ccgccaacgc gcgatgaact ggtcaaactc attgccgata
    15841 tggggatttc cgtacgcgcg ctgctgcgta aaaacgtcga accgtatgag gagctgggcc
    15901 ttgcggaaga taaatttact gacgatcggt taatcgactt tatgcttcag cacccgattc
    15961 tgattaatcg cccgattgtg gtgacgccgc tgggaactcg cctgtgccgc ccttcagaag
    16021 tggtgctgga aattctgcca gatgcgcaaa aaggcgcatt ctccaaggaa gatggcgaga
    16081 aagtggttga tgaagcgggt aagcgcctga aataaagcgg cgatatccac tgccgatagt
    16141 tggtaaagtg cgctttgttt atgtcggatg cgacgctaac gcgtcttatc cgacctacaa
    16201 aaccgtgcaa attcaatata tggcatgaaa tatgtagagc gtagcgcatc aggcaatttt
    16261 gctatttacg cagcactcct cgcgtaagaa ttcaattatc gcttccagca ttgcgtattg
    16321 cgaaacacac atcagcgtcc gactctcacg gttttgtttg accagtccta ccgataccag
    16381 tgctgatata tggtggctga gtgtggagcc aggtatccca agctgctttt gcaactcacc
    16441 gaccggcaac ccttgttccc cggctttgac aagatgttta aatatgaaca aacgggtcgg
    16501 gtgacctagc tctttcagcg ctttagcgac ttcttccaac tgcatatgac acccctctgt
    16561 ctggttgata tttcgataat accagaaata tcttgatctg aatttcattt cgattattct
    16621 ggaaatatag atttatccaa tgaggttacg tatgagtagt tggcttgcga tgctgcaaga
    16681 tgccgcagag atgtttgtgt ttctcgccgt cgagctttcg ttgctgttta tagtgattag
    16741 tgccggtgtc agcctgataa gacaaaaggt gccagaccat aaaatccagc agatgatggg
    16801 ggccagaaaa gggagaggct atctcctggc tgctctgttg ggagccgtta ccccgttctg
    16861 tagttgctcg acaataccca tgttacgtgg attgttgtca gcgaaagccg ggt
//
LOCUS       DAZPBM010000086        16866 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPBM010000086 DAZPBM010000000
VERSION     DAZPBM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.086"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            93..1133
                     /locus_tag="ACHUZQ_002541"
     CDS             93..1133
                     /locus_tag="ACHUZQ_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1026207.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="ACHUZQ_002542"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="ACHUZQ_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1026208.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="ACHUZQ_002543"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="ACHUZQ_002543"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1026209.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="ACHUZQ_002544"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="ACHUZQ_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1026210.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="ACHUZQ_002545"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="ACHUZQ_002545"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1026211.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="ACHUZQ_002546"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="ACHUZQ_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1026212.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9132)
                     /gene="ygeA"
                     /locus_tag="ACHUZQ_002547"
     CDS             complement(8440..9132)
                     /gene="ygeA"
                     /locus_tag="ACHUZQ_002547"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1026213.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9263..10681)
                     /gene="araE"
                     /locus_tag="ACHUZQ_002548"
     CDS             complement(9263..10681)
                     /gene="araE"
                     /locus_tag="ACHUZQ_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1026214.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10996..11757)
                     /gene="kduD"
                     /locus_tag="ACHUZQ_002549"
     CDS             complement(10996..11757)
                     /gene="kduD"
                     /locus_tag="ACHUZQ_002549"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1026215.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11787..12623)
                     /gene="kduI"
                     /locus_tag="ACHUZQ_002550"
     CDS             complement(11787..12623)
                     /gene="kduI"
                     /locus_tag="ACHUZQ_002550"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1026216.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12910..14091)
                     /locus_tag="ACHUZQ_002551"
     CDS             complement(12910..14091)
                     /locus_tag="ACHUZQ_002551"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1026217.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14346..15575
                     /locus_tag="ACHUZQ_002552"
     CDS             14346..15575
                     /locus_tag="ACHUZQ_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1026218.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16035..16667
                     /locus_tag="ACHUZQ_002553"
     CDS             16035..16667
                     /locus_tag="ACHUZQ_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1026219.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         4451 a         4423 c         3904 g         4088 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacacac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtaag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggccta gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacacggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
     3421 gctgctgttt tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 agtcaatcgc aacagcattt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacaccg gtaatttttt attgattggc aatggttacc acaacgaaca
     5401 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggcttcga aaaccgttgt attgctctgg acgatcgtta
     5581 cggtgcctgg ctggctacgc gtcatttaat tcaacaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggtgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacaatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat agattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
     7681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cactcaccgc actggattat gcggcaagcg
     8281 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
     8521 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt
     8701 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
     8881 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
     8941 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa
     9001 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
     9061 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
     9121 aattgttttc attcctctct ctcctgatgt gtgttaccgc aacaaggctg aataaggaac
     9181 gagacaaacg cctcaacggc caggtgccca atctctatta acgaaaaaag ggccggatgt
     9241 acagcacatc cggcccgtga aatcagacgc cgatatttct caacttctcg cctgccatca
     9301 gtttgcgttc gatatgttcc agcgtgacat ttttggtttc cggaatgagc cagaaagtga
     9361 tgcccacaaa cgcaatgttc agcgcagtgt agagccagaa cgtaccggca gcaccaatgc
     9421 tgtcgagcag cgtcaggaag gtcgcgccga taatcatatt cgacacccag ttcgtggtgg
     9481 tcgaacaggt aataccgaaa tcgcggcatt tcagcggctg aatttcagag cacaggatcc
     9541 acaccactgg cgcggcgctc atcgcataac cggcaataca catcatcgtc atgccaacag
     9601 agagccagga caagccactg gaagccgtac cgttatcaaa ctgcatcagg caatagccca
     9661 gcaccagagt gcctaacgcc atcacgctga aaccaatttt cagagccggt ttacgccctg
     9721 ctttatctac cgtaaacacc gcaataaagg tggcgaacat aaaggtcagc cctacgacca
     9781 gagtcgcaat catctgttgt tctgtggtcg taaagcccgc cattttgaag atacgcggcg
     9841 cgtagtacat gatgatgttc ataccggtaa actgctgcat cgcctgcaac aacataccga
     9901 gaaacacagc acgacggacg ttacggttga tcttaaacag tgcccaaccg ccctgtttta
     9961 acttcaggct ttcacgaatt tcgttgagtt cttctcgcgc tttttccgac gtatcgcgca
    10021 gcatacgcaa cacttcttcc gcctcaatat gacgcccctt ttccgccagc cagcgcgggc
    10081 tatttggcag gaagaccacc agaataatca gcaggactgc tggtaaagca agaaccccca
    10141 acattgcgcg ccagttaccg ctataactga acgctgtatc ggataaaaac gccagcacga
    10201 tgccgagtgt gaccatcaac tggtacatac tgatcatctt accgcgaacg ttttcacttg
    10261 ccatttcaga aagatacaga ggagcggtgt aagacgcgat cccgacagca atgcccagca
    10321 ccacccgagc ggcgattaac atctctacgc tggtcgcaaa agcggaccct atagaaccga
    10381 gtacaaacag gatggccccc gccatcaggc tgtatttacg ccccaggcgg aacgacagcc
    10441 aaccattaaa cagcgcacca attgctgcgc cgagcatcat gctactaacc acccattcct
    10501 gcaaacgact ggtcagcaca aagtgatcgg taatgaacgg caacgctccg gcgattacgc
    10561 cgatatcaag accaaataac aatcctgcga ccgcagcagc taccgaaaca aacatattca
    10621 tacgccgcgt atcccgcaaa gaacgtggcg ttaaagcaga ttccgtattg atagtaacca
    10681 ttttttcctg ccagcagaga gtaagacata gtgaaaaaat acgtgaacaa ctcacacagg
    10741 tgtcaggtcg gaaacagcat aaatatggat taaattgctg cgacatgtcg ttatgtgatg
    10801 gatattccaa ttttcaaatt aagttgaatt attgagatta ttattaacca cctaatttta
    10861 cagcagataa aattcataaa gttcattaat tgataattga tatggattat ttcataacca
    10921 tgatatgaat tatgatgacc tacaggtata aaaaaccctg ccatgaggca gggtcataaa
    10981 agtaagagga atgaattaac gagccagcca accgccatct acggcaatgg tataaccatt
    11041 cacataatct gaagcgctgg aggcaaggaa cactatcggc cccatcaggt cactcggcag
    11101 tccccaacga ccagctggaa tgcggtcgag aatttccgcg ctacgttgtt catctgcccg
    11161 tagttgttga gtattgttgg tcgccatgta acccggagct atcgcattaa cattaatgtt
    11221 gtgtttagcc cattcgttcg ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc
    11281 ggtataagaa ggcacacgga tcccgccctg gaaggagagc attgacgtga tattgataat
    11341 cttgccgcca ttgcgttgcg cgataaagtg tttcgccgct gcctgagaca tgaagaatac
    11401 gctcttgata ttcaggttca tgacatcgtc ccagtctttt tcgctgaact cgagagcatc
    11461 ttcgcggcga atcaatccgg cgttattcac caggatatca atatgaccaa actcagctac
    11521 cgcgcgatcc agcagtgctg gaatgccatc aatctttcgc agatcggcgg tcaggcttaa
    11581 aaaacgacgc cccagcgccg tgacctgctt gatggtttca gtcggttcaa cgatgttaat
    11641 gccaacaatg tcacagcccg cttgcgccag ccccaacgcc atcccctggc ccagtccagt
    11701 atcacaacca gtgacgaccg caactttacc ttcgagagaa aatgcactta aaatcataac
    11761 aataccttat tcgtttatgc cgacaactag cgcaaatctt taacggccac atggtccata
    11821 tcatcaaaga cctggttttc accaaccatg ccccagataa aggtataagc tttggttccg
    11881 acgccggaat ggatcgacca gctgggggag atcaccgcct gctcgttatg catcacaatg
    11941 tgacgcgttt cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg
    12001 aaatagaaat aaacttccat ccggcgctcg tgagtgtgac acggcatggt gttccacaag
    12061 ttacctggag ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg
    12121 acaaaatatt tgttaattgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga
    12181 gatacttcgt ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg
    12241 taataaaact ttgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct
    12301 ttaccaacat acagggcgtc gcggtgaccg atttcatagc attggccatc gacagtaatc
    12361 gtaccggcac cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg
    12421 ccgagttgtt taccaacttc cccgccaacg gaaaccgttt tagttatcgg cataatgccg
    12481 ccaacaataa ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc
    12541 ttttcaacca aaaattcatt gcgcagccct tgggtatcca gcgtttttgc gtgcgcactg
    12601 tggatgccct gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa
    12661 aacgatagtg aaacactgtt tcgttttctc tataaaggga tgatatgccc atgggctagg
    12721 gttttcaatt aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag
    12781 tgtcgatcac gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg
    12841 acttgccagc catcattatt caacagatta atgacggcag atatttatcg gtaaggagga
    12901 aatgcagagc tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca
    12961 aagcgttgcc agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag
    13021 gattcggcaa ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt
    13081 gacccgacgc tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc
    13141 ttcattagcc tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg
    13201 gcgggtcgca tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa
    13261 tgcgcggatg cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat
    13321 cattaccgca gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag
    13381 actatcaaat gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg
    13441 ttcatcggta tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc
    13501 atctttaaat cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta
    13561 agcatcctgc aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc
    13621 aatatgataa tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg
    13681 gctattgcca agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat
    13741 gttttcctgg ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc
    13801 cagatgcagt gctttaagtc cggaaccgca aacgtcatta atcgtgattg cagaaacgct
    13861 attaggaaga ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt
    13921 caacacctga ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat
    13981 taacgctttc acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccacg
    14041 aaagcagccg ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc
    14101 gcaccaatta acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa
    14161 aaaagtgatt tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca
    14221 cgtaaggttt gcaaccacta acccaccaat agatgggtag ataaggcgtt aatctcccat
    14281 acttaacctg gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc
    14341 agataatgtc taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca
    14401 cagccgttgg tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg
    14461 tcctgtttat tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat
    14521 gccagttcat cctctcatca aaaacatcag catgtgaagg gataacgggc gcggtaacac
    14581 actactatgg caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg
    14641 tcgtcgtgtt gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc
    14701 atatggttat tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga
    14761 atctcatttt tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc
    14821 cattgattgc ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc
    14881 tattaacgac ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc
    14941 ccgtgatggt tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac
    15001 gtgaaaaata tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc
    15061 tcatcatctg cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt
    15121 cgtatattga agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc
    15181 cgaacgcccc agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga
    15241 aatcattcct gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa
    15301 cactacagca ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt
    15361 tggtatcgct gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt
    15421 atgcgatcag cggcccgctc attgccatga tacttttcat catgcctacg ttgtcaacgt
    15481 atctgatccc ggcgcttaaa ccctggcgct ccatcggaaa tctgattacg ctgatcgtgg
    15541 gtatcctgtg cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt
    15601 gctacaattt tgaaccattc catttttcat aatattcatt tgttattatt ccgccctatc
    15661 acttctctcg tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat
    15721 cttaaataag actatttaag atgcataact tagattcgca agatatcttc tggtcattca
    15781 aaaacaattt gcgatataga tattctcata ccatgagata gttatctgga ccacgttcga
    15841 ccatgcaaga ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa
    15901 atgtcttaat cagaattata gcaaatacag attaatcgca atttttccca gcaaaattac
    15961 aaaatcaaga tgtattgaca tcatcaatca ccaatgaaat tatgcaatta tatacggata
    16021 gggaggttct taacatgggg ctttgtagtc gttataaaag tcttacatgc aatagttgct
    16081 caatgcattg ccaaataatg ccagaagagt caccgcgttt gcagtattgc gcaaactcgt
    16141 gtttttgtat gtggcccgaa gaaagttcat attttaatcg tggtgtggtg gaaggtatct
    16201 taacaaaaaa ccacaatgcg agattaagcg gatatatttt tgtggatttt tcagtaagtt
    16261 ttttacgtct attccttgaa aaagactgga ttgactatct tgcaagtact gacatgggga
    16321 tagttttagt cagcgaccgc aatatgcaat cgttagctaa ctactggcga aaacacaaca
    16381 ccgcaatatc tgctgtcata tataatgatg atggtcttga tgttgccaat gaaaagatca
    16441 gacaactatt tattggtcgt tatttatcat ttaccagagg taacacatta actcagatgg
    16501 aattcaccat aatggggtat atggtttctg gttataatcc atatcagatt gctgaagtgc
    16561 tggatatgga tatccgtagc atctacgcgt acaagcaacg aatcgaaaag agaatgggtg
    16621 gtaaaataaa cgaattattt attcgttcac attcggtcca acattgatac tcaacgacca
    16681 gccagaatca tactctgttc atacgggcat ctattgaaat gcccgtattt tataaataag
    16741 cagtaacccc aagagtaaac aacatagtat ttaaaaccat cacacgaaaa attcagaagc
    16801 attacgaaat atgaatttac aaacaatagt ggcataaatg ttaaccatgt taatttacgt
    16861 aaagtt
//
LOCUS       DAZPBM010000087        16402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPBM010000087 DAZPBM010000000
VERSION     DAZPBM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.087"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHUZQ_002554"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHUZQ_002554"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR1026220.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHUZQ_002555"
     CDS             complement(2380..2820)
                     /locus_tag="ACHUZQ_002555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1026221.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHUZQ_002556"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHUZQ_002556"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1026222.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHUZQ_002557"
     CDS             3538..4236
                     /locus_tag="ACHUZQ_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1026223.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHUZQ_002558"
     CDS             4465..5673
                     /locus_tag="ACHUZQ_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1026224.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHUZQ_002559"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHUZQ_002559"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1026225.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHUZQ_002560"
     tRNA            7780..7856
                     /locus_tag="ACHUZQ_002560"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHUZQ_002561"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHUZQ_002561"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHUZQ_002562"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHUZQ_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1026226.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHUZQ_002563"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHUZQ_002563"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1026227.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHUZQ_002564"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHUZQ_002564"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1026228.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHUZQ_002565"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHUZQ_002565"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1026229.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14612
                     /gene="dppF"
                     /locus_tag="ACHUZQ_002566"
                     /pseudo
     CDS             13606..14612
                     /gene="dppF"
                     /locus_tag="ACHUZQ_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
     gene            complement(14642..15913)
                     /locus_tag="ACHUZQ_002567"
     CDS             complement(14642..15913)
                     /locus_tag="ACHUZQ_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1026230.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4096 c         4409 g         3997 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccccggac gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa
    14461 gcccactgaa tccaccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc
    14521 cctgcaccca gttgcagccg cagctaaaag actacggcgg tcaactggta gcttgttttg
    14581 ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca tgcgctccgg
    14641 tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtac agcagaatca
    14701 gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc aggaaaaacg
    14761 gaatgaggca cgacacaata ccatataacg ggcttcccaa ctgaaagaac accagcaccg
    14821 atacacgaaa cgaaacccaa atcgtcaacg taatgacgat aaaagcgcag atcgccagag
    14881 tcagcacgcg tgagttaatt ttcttggtat caataattcg gcttaacaga ttgagaataa
    14941 tgcctttaat ggcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa
    15001 agatattaag caccgtagag gtgatatgaa tgatatgccc agggatcacc tgcgcggcca
    15061 gcgccagtgc tgagatattt tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga
    15121 aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa ctaattcggt
    15181 gggtacgcag cgcgagccgg gttgccagta ctttatccgc ttcccgttta cgataggcaa
    15241 tattcattgg gttaagtacc tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa
    15301 gcaagacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg gcgaaattcc
    15361 agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc accaccattg
    15421 gcccggaaat cttaaacagt aatcgttcac caccagacgc aatcgccacc agtacggcga
    15481 aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa ccgaaggttt
    15541 tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc ccgtggataa
    15601 tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg taatgactga
    15661 taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa taaatgtcct
    15721 gcactaccca ggtggcagga taagcaatga tcgctgcggt aataaatacc caaattccct
    15781 tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg cctatgcata
    15841 ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg
    15901 tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc
    15961 agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac
    16021 agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat ttcactacca
    16081 gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt aagctggaag
    16141 gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc
    16201 ggcaagccga aagggttccg gtgaggcgca atgttgcggg ggctttatcc ctggtggcat
    16261 tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gtacctgaca acataacggg
    16321 ggctaatctt gaacctagac cactcaagaa tagccgcgaa acgttgtcat tacaacacag
    16381 gcggctatat gacgctcgca ga
//
LOCUS       DAZPBM010000088        16219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPBM010000088 DAZPBM010000000
VERSION     DAZPBM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.088"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(50..1252)
                     /gene="ubiI"
                     /locus_tag="ACHUZQ_002568"
                     /gene_synonym="visC"
     CDS             complement(50..1252)
                     /gene="ubiI"
                     /locus_tag="ACHUZQ_002568"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1026231.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1275..2453)
                     /gene="ubiH"
                     /locus_tag="ACHUZQ_002569"
                     /gene_synonym="visB"
     CDS             complement(1275..2453)
                     /gene="ubiH"
                     /locus_tag="ACHUZQ_002569"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1026232.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2450..3775)
                     /gene="pepP"
                     /locus_tag="ACHUZQ_002570"
     CDS             complement(2450..3775)
                     /gene="pepP"
                     /locus_tag="ACHUZQ_002570"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1026233.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3801..4379)
                     /gene="ygfB"
                     /locus_tag="ACHUZQ_002571"
     CDS             complement(3801..4379)
                     /gene="ygfB"
                     /locus_tag="ACHUZQ_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1026234.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4547..4876
                     /gene="zapA"
                     /locus_tag="ACHUZQ_002572"
     CDS             4547..4876
                     /gene="zapA"
                     /locus_tag="ACHUZQ_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1026235.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4918..5101
                     /gene="ssrS"
                     /locus_tag="ACHUZQ_002573"
     ncRNA           4918..5101
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUZQ_002573"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5122..5724
                     /locus_tag="ACHUZQ_002574"
     CDS             5122..5724
                     /locus_tag="ACHUZQ_002574"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1026236.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5825..5884)
                     /locus_tag="ACHUZQ_002575"
     CDS             complement(5825..5884)
                     /locus_tag="ACHUZQ_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1026237.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6216..6275)
                     /gene="ibsC"
                     /locus_tag="ACHUZQ_002576"
     CDS             complement(6216..6275)
                     /gene="ibsC"
                     /locus_tag="ACHUZQ_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1026238.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6504..7736)
                     /gene="serA"
                     /locus_tag="ACHUZQ_002577"
     CDS             complement(6504..7736)
                     /gene="serA"
                     /locus_tag="ACHUZQ_002577"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1026239.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(7992..8651)
                     /gene="rpiA"
                     /locus_tag="ACHUZQ_002578"
     CDS             complement(7992..8651)
                     /gene="rpiA"
                     /locus_tag="ACHUZQ_002578"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1026240.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9081..9974
                     /gene="argP"
                     /locus_tag="ACHUZQ_002579"
     CDS             9081..9974
                     /gene="argP"
                     /locus_tag="ACHUZQ_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1026241.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10177..12321
                     /gene="scpA"
                     /locus_tag="ACHUZQ_002580"
     CDS             10177..12321
                     /gene="scpA"
                     /locus_tag="ACHUZQ_002580"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1026242.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12314..13309
                     /gene="meaB"
                     /locus_tag="ACHUZQ_002581"
     CDS             12314..13309
                     /gene="meaB"
                     /locus_tag="ACHUZQ_002581"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1026243.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13320..14105
                     /gene="scpB"
                     /locus_tag="ACHUZQ_002582"
     CDS             13320..14105
                     /gene="scpB"
                     /locus_tag="ACHUZQ_002582"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1026244.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14129..15607
                     /gene="scpC"
                     /locus_tag="ACHUZQ_002583"
     CDS             14129..15607
                     /gene="scpC"
                     /locus_tag="ACHUZQ_002583"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR1026245.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15604..>16219)
                     /locus_tag="ACHUZQ_002584"
     CDS             complement(15604..>16219)
                     /locus_tag="ACHUZQ_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1026246.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3931 a         4374 c         4132 g         3782 t
ORIGIN      
        1 gggagatgaa agtgtgatgg gtatcaaata aacagcagaa gagaaatttt taacgcagcc
       61 attcaggcaa atcgtttaat cccattgcct ggcggataag ttgtggttta acgccaggaa
      121 gcgtgtcggc cagtttcaaa ccaatatcac gcagcagttt tttcgccgga ttggtaccgg
      181 aaaacagatc gcggaatccc tgcataccag ccagcatcag cgccgcactg tgcttgcggc
      241 tacgctcata ccggcgcagg taaatgtact gcccgatgtc tttcccctga cgatgcaacc
      301 gtttcagttc agcaatcagc tctgcagcat ccataaagcc aagatttact ccctgccccg
      361 ccagcgggtg aatggtatgc gcggcgtcgc ccaccagcac cagacggtgc gcggcaaact
      421 ggcgcgcata acgccccgtc agtgggaaca cctgacgcgc gctctcaacc ttgcataagc
      481 ccaggcgatt atcaaaagcg atatttaacg cgcgattaaa ttcatcttca cttgcctgct
      541 gcatccgctg cgcttcctct ggcgacagtg accagacaat tgagcaaaga tgcggatcgc
      601 taagcggtaa aaaggccaga atgccttcgc catggaaaac ctgccgcgcc accgcatcat
      661 gcggttcttc cgtgcgaatg gtcgctacca gcgcgtgatg ctgataatcc cagaaagtca
      721 gcggaatatc tgctttgttg cgcaaccagg aattagcacc gtccgcgcca atcaccagac
      781 gcgccgttaa catgctgcca tctttcagcg tcaggaaagt ttcattttct ccccaggcga
      841 cctgctgtaa ttctgcgggg gccaacagag tgatatctga cgactgctgc gctttgttcc
      901 acagcgcgta gtgaatcact gagttttcaa cgatatgccc aagatggcta tagcccatgc
      961 tttgatcgtc aaacgaaatg tgaccaaaac tgtctttgtc ccacacttcc ataccgtgat
     1021 aacaactggc cctacgagag agaatgtcct gccagacgcc aagacgggtg agtaattttt
     1081 cgctggcggc attgatagcc gaaacgcgca gttgtggtgg tgcatccgcc gccagaggtt
     1141 ccggtacgcg ctgctccagt acggcaacgc gtaagccgct cccctgtaag ccgcaggcaa
     1201 ccgccagccc caccatgccg ccaccaacaa tggctacatc aacactttgc attgtttatt
     1261 ccttaaaacc gccttcaacg cgccacccaa ccgagggtgc gctgcgccag cacatcgcgt
     1321 gccggggtga ataattccat cgtcatcagt ccgatgttgc gcccgacaac cagcggtgcc
     1381 caacggttgg caaaaagatg taccaggctg tccgtgacgc cgatggttgc ttcgcgatcg
     1441 ctctgtcgac gctgctgata gcggcacaat atgccgtaat caccgatgtc ttctccgcgc
     1501 tcatgcgcct gagtcagggt ttccgcaagg ctcatgacat ctcgcatacc gaggttaaac
     1561 ccttgcccgg caatcgggtg cagagtttgc gccgcattgc ccaccagcac ggtacgatgg
     1621 gtaatagctc tggcggcgcg ggttaacgcc agcggataag cactgcgttt accggcgtgg
     1681 gtaatttttc caagtcgcca gccaaaggcc gactggagtt cacggcaaaa cttctcgtcg
     1741 ctccacgaca gcacctcttc gcgccgttcc agtggatgac accagaccag cgaacagcgt
     1801 ccgtcagaca tcggcaacat cgccagcggg ccatgttgcg tgaagcgttc aaaagcgcgg
     1861 ccttcgtgcg caacggaagt cgcaacgttg gcaatcacgg ccagttgttc gtaaggctcc
     1921 tgctgccagt caacgccgca cgcggtggct aacgctgaat gggtgccatc agctgctacc
     1981 agcacgcggc ccgtcagcgt ctcgccactc tccagcgtca cttcaacgtg actctgagta
     2041 cgggcaacgt tagccacgcg atcagggcaa tgcagcgtta cgccaggtgc tttacgcagc
     2101 aatgcaaaca gccgttgccc gacattgtgc aattcgacaa cctgtcccag cgccgccagt
     2161 tggtaatctt ctgcggcgag ggtgacaaat ccagcgtgac cacgatcgct gacatgcacg
     2221 gtagtgatgg cggttgcgta atccgccaga gattgccaga cgccgatgcg cgccagttgc
     2281 tgacaggtcc ccgcagccag cgcaatagct cgtccatcaa agcccggatg agcatgtgac
     2341 tctggcgcgg tcgcttcaat caaatgtacc ggcagcgccc cgtgacttaa ccgggaaata
     2401 gccagcgcca gcgtcgcgcc cgccatgccg ccaccgacga tgattacgct cattgctttc
     2461 tcgcagctgc catcaacgct tcgatttctt ccggcttttt caccacgctg gcggtgaggt
     2521 tttcgttgcc ggtttcggta atcacaatgt cgtcttcaat acgaatgccg ataccgcgat
     2581 attgttctgg cacatccgca tccggcgcga tatacagacc aggctctacg gtcagtacca
     2641 tccccggttc cagaatgcgc gagcgatcct gaccataaac gccaacgtca tggacatcca
     2701 gtcctaacca gtggctaagg ccatgcataa agaaaggacg atgggcgttc tgagcgatca
     2761 gttcatcaac atcacctttc aggatgccga gttttaccag gccgctaacc atgatgcgca
     2821 ccacttcacc agtgacttcc agaatggaag ttcccggacg atacaggcgc aggctggttt
     2881 cgagagactc cagcacaatg tcgtagattt cacgctgggc ctgggtgaat ttgccgttga
     2941 ccgggaaggt gcgggtaata tcgcccgcgt aacctttgta ttcacaaccc gcgtcaatca
     3001 acaccaggtc accgtcgcgc agttcactct cgttttcggt gtagtgcaga atgcagccgt
     3061 tttcaccgct gccgacaatg gtgttatagg acggatagcg cgcaccgtgg cggttaaatt
     3121 cgtggtgaat ttcgccttcc agatggtact cgaacattcc cggacggcat ttttccatcg
     3181 cccgtgtatg tgccatggcg gtgatttctc ccgcgcggcg gagtacggca atctcttctg
     3241 gcgatttgaa caggcgcatt tcatgaacaa caggacgcca gtcgatcatc gttgccggtg
     3301 cggtgagatt ttgccgcgaa cctttacgca gtttttccag cgcactgttc atgatttcat
     3361 cagcatatgc atattcgccc tgggcatggt aaaccacatc caggccgtta agtagttgat
     3421 aaagttgctg attgatttcg ctgaatgcca gtgcgcggtc aacgcccagt ttctctggcg
     3481 cggcatcctg gcctaagcga cggccaaacc agatctccgc cgtcaggtcg cgaacgcggt
     3541 taaacagaac gctgtggtta tgagtgtcat cgcttttaat cagcaccagc accgcttccg
     3601 attcgttaaa gccggtgaag taccagaagt cactgttctg acgatagggg tattcgctgt
     3661 cggcgctacg tgttacttct ggtgcagcaa aaatcagcgc ggcgctgccg ggttgcattt
     3721 gctccaccag ggcctgacgg cgacgctgaa actcttgccg ggatatctca ctcataacac
     3781 tctccttacg ttttttgttt ttagtgtaga gtcggttttt gtacttctgg cgcggtcggt
     3841 tgcggatgag taaaggtgtc gtggcataac agcgcggcaa cacgaacgta ttcgatgatc
     3901 tcttcaagcg acatttcaag ctcttcctga tcttcgtctt cgtcgtaacc cagttgcgca
     3961 atgttacgca gatcgtcgat agcttcaccg gtttcgccgg tcactttatc cagcttcggt
     4021 tgcgtaacgc caagaccaag caggaagtga ttgacccaac ctgccaatgc atcagcccga
     4081 tcgaaaacgc tgacatcatc gccatcaggc agataaagct gaaaaaggaa gccgtcatcc
     4141 tgcagggcat cgctggtggc agagtgcatt ttacgcagtg cctgtgccag ctcatgaccg
     4201 aaagccatgc cttcgttcgt caggtcgtga agtagcggca gccatgagct gtcatcgtta
     4261 ccgccacata tcatcccact gattaaacca tgcatctcgg caggggtcag ccccgtccct
     4321 tgttggttca gatactggtt catttcgttg taaccaggca tttcgttctg tatagacata
     4381 agcattcgtc gtcaaaggga ggaatattca tgatatgcta ccactttggg ccctggtgga
     4441 ccagaaaagg gcttgtctct tctcatcagg gtagctatag tgtcgcccct tcgcagacca
     4501 tgggtctaaa gacgaaggca gcgcagtcaa tcagcaggaa ggtggcatgt ctgcacaacc
     4561 cgtcgatatc caaatttttg gccgttcact gcgtgtgaac tgcccgcctg accaaaggga
     4621 tgcgttgaat caggcagcgg acgatctgaa ccaacggttg caagatctga aagaacgcac
     4681 tagagtcaca aatactgaac agttggtctt cattgccgca ttgaatatca gctatgagtt
     4741 agcgcaagaa aaagcgaaga ctcgtgacta cgcggcaagt atggaacagc gtattcggat
     4801 gctgcagcag accatagaac aagcgttact tgaacaaggt cgcatcaccg aaaaaactaa
     4861 ccaaaacttt gaatgacact tttcggttta ctgtggtaga gtaaccgtga agacaaaatt
     4921 tctctgagat gttcgcaagc gggccagtcc cctgagccga tatttcatac cacaagaatg
     4981 tggcgctccg cggttggtga gcatgctcgg tccgtccgag aagccttaaa actgcgacga
     5041 cacattcacc ttgaaccaag ggttcaaggg ttacagcctg cggcggcatc tcggagattc
     5101 ccttcttatc tggcaccagc catgacgcaa ctaccagaac tcccactgac attatcccga
     5161 caagaaatcc gcaaaatgat tcggcaacgt cgtcgtgcgt taacgccgga acaacagcag
     5221 gaaatgggtc aacaagccgc tacccggatg atgacttatc ccccggtggt gatggcgcat
     5281 acggtcgctg tattcctctc ttttgatggt gaactcgaca cccagccgct aatagaacaa
     5341 ctctggcgcg ccggtaagcg cgtatatctt ccagttttgc atccctttag tgccggtaat
     5401 ttgctgttcc tgaattacca tccgcaaagc gaactggtga tgaacaggtt gaagatccat
     5461 gagccaaaac tggatgtgcg tgacgtgcta cccctttccc gattagacgt gctgatcaca
     5521 ccgctggtcg cctttgatga gtacggtcag cgcctgggaa tgggcggtgg tttttatgat
     5581 cggaccttac aaaactggca gcactataaa acgcaaccgg tgggttatgc gcatgattgt
     5641 cagttggtgg aaaaactccc cgttgaagag tgggatatcc ctcttcctgc ggtggttaca
     5701 ccgtcgaaag tctgggagtg gtaagggcga tacacctgca tcgccctgat tgacatcgtt
     5761 gattctttaa cctaatttag tgagtaaggg taagggagga ttgctcctcc cctgtgactg
     5821 actgttaata agctgggaaa cttatgagta acagtacaat cagtatgatg gcaagtcgca
     5881 tcataaccct tctccttcaa gccctcgctt cggtgagggc tttaccgtta cagccccatg
     5941 ctgcccagct atcgtaagct ctctttaaat aaccacaacg cattgatcta cctttaaatc
     6001 ttttcttcga gcgtgctcga aaaacccatg caaaacagag ataatttttg ggaaaaagga
     6061 tctattacgg cagtaaacgt gggcgatgaa gtggctatca aaaagttcgg ttcagggcga
     6121 tgcaccccgc atcgccctga ttgacatcgt tgattctttg acctaattta gtgagtaagg
     6181 gtaagagagg atgactcctc ccctgagact gactgttaat aagctccgaa acttatcagt
     6241 aacagtacaa tcagtatgat gacaagtcgc atcataaccc ttctccttta agccctcgct
     6301 tcggtgaggg ctttaccgtt acagccccat gctgtcccgc catcgtaaat ccccattaaa
     6361 taaacacaac gcattgatct gagtttgatt tattttctgg agcagactcg caaagtagaa
     6421 tgcgcaacgc ggcaacggtg tggagaaggg ataaaaaaac gggcaagtca gtgacctgcc
     6481 cgttaatttt cagagaaggg gaattagtac agcagacggg cgcgaatggt acccggaata
     6541 gctttcattg cctgcagcgc tttttcggca acgtcttcgt cagcttcaat atcaataact
     6601 acataaccca tctgggcgga agtttgtaga tattgcgcgg cgatgttgac gccctgctcg
     6661 gcgaagattt tgttcagcgc agttagcacg cccggacggt tttcgtggat gtgcatcaga
     6721 cgacgaccac catgcagtgg cagcgagact tccgggaagt tcaccgcaga gagcgttgag
     6781 ccattgtcag aatacttgat caatttaccc gcaacttcca ggccgatatt ctcctgcgct
     6841 tcctgagtcg aaccgccaat gtgtggcgtc agaaggacgt tgtcgaattc acacagtgga
     6901 gaggtaaatg gatcgctatt ggtcgccggt tccgtcggga atacgtcgat tgccgccccc
     6961 gccagatgtt tgctcgccag cgcatcacac agcgccggaa tatccaccac agtaccgcgc
     7021 gaggcattaa tcagcagcga gccgggcttc attagcgaaa tctctttcgc gcccatcata
     7081 tttttggtgg acggattctc tggtacatgc agactcacca catcgctcat attcagcagg
     7141 tcagaaagat gctgtacctg agtggcgttg cccagcggca gtttgttttc aatatcataa
     7201 aagtaaacat acattcccag cgattcagcc agaatgccca attgcgtacc aatatgaccg
     7261 tagccgatga tacccagctt tttgccgcgc gcttcaaaag aacccgccgc cagtttgttc
     7321 cacacgccac ggtgcgcttt agcattggct tccggcacgc cgcgcaatag cagcagcagt
     7381 tcgccaatca ccagctccgc aacagagcgc gtatttgaga acggtgcgtt aaataccggg
     7441 atcccgcgct ttgccgccgc atccagatca acctggtttg ttccgataca gaaacagcca
     7501 atagcgacca gtttttctgc ggcgttgatc acgtcttcag tcagatgggt acgggatcgc
     7561 aggccgatga agtgggcatc gcggatggat tcttttaatt gttcatcatc cagcgcgcct
     7621 ttgtgaaatt cgatgttggt gtaaccagct gcacgaaggc tttccagcgc cttttggtgc
     7681 acgccttcta ccagcagaaa cttaatcttg tctttctcca gcgatacctt tgccatttac
     7741 ccaatcctgt cttttgaaat gttgtgtgcg gatttgcatc cgcctttcaa catatcaaaa
     7801 aataatattg cggcaatatg aacgtttgcg tcgcgatgtt gaggaaatat cacgcaaagg
     7861 tatttcttgg ttgaatatgc tggtttagag gatttattta gagcaatcga caatagccaa
     7921 gtaaaaacgt gacacatgtc accaaattta atgaagagaa tttttttaac gggggaggtt
     7981 cccccgtcag atcatttcac aatggttttg acaccgtcag gtgtgccaat cagcgcaacg
     8041 tccgcgccac ggttagcaaa caagccaaca gtcaccacgc caggaatcgc atttatggcg
     8101 ttttccatcg ctatcgggtc aaggatttcc atgccgtgga cgtcgaggat cacgttgccg
     8161 ttatcggtca ccacgccctg acggtattcc ggacgaccgc ccagtttcac cagctggcgc
     8221 gccactgcac tacgtgccat cgggataact tctactggca gcgggaattt gcccagaata
     8281 tcaacctgct tggaagcgtc agcaatacag ataaattttt ccgcaaccga agcaatgatt
     8341 ttttcacggg tcagcgccgc gccgccgcct ttgatcattt gcatgtggcc gttgatttca
     8401 tctgcgccat caacgtagat gccaaggctg tcgacttcgt tgagatcaaa aacgtgaatg
     8461 ccgaggcttt tcagtttttc agtggaagca tctgaactgg aaacggcccc ttcaatctgg
     8521 cctttcattg taccgagcgc gtcaataaag tgtgcggcgg tggaacctgt acctacacca
     8581 acaatggtgc cgggctgaac atactgaagt gccgcccatc ctactgcttt tttcaattca
     8641 tcctgcgtca tgatcgtttc gcctgtggta tgaaatttca cacgcattat atacaaaaaa
     8701 agcgattcag accccgttgg caagccgcgt ggttaactca tccataaaat atcgcgcaat
     8761 ggcgggcatc ccctttcgcc ccgcaaataa agcatacaac ggtctgggta tgccgctcca
     8821 cggtgcgaac aggcgcacca gttccccccg ttcgcaagcc cctgtttaca ggcaaattga
     8881 ggcaataacg ccaccccatt caacacaacc agggcacgaa ctcgctgagc gaaatgcatt
     8941 agcgcaaatc cctgatcttt taagctgcgc gtttaaccac ggatacaaat gttacccgcc
     9001 gacggcttcg gtatatgcaa cctgacacaa aattgtgtca tagtgcagga aaaagcattt
     9061 accaggagca gacaacagca atgaaacgcc cggactacag aacattacag gcactggatg
     9121 cggtgatacg tgaacgagga tttgagcgcg cggcacaaaa gctgtgcatt acacaatcag
     9181 ccgtctcaca gcgcattaag caactggaaa atatgttcgg gcagccgctg ttggtgcgta
     9241 ccgtaccgcc gcgcccgacg gaacaagggc aaaaactgct ggcactgctg cgccaggtgg
     9301 agttgctgga agaagagtgg ctgggcgatg aacaaaccgg ttcgactccg ctgctgcttt
     9361 cactggcggt caacgccgac agtctggcga cgtggttgct tccggcactg gctcctgtgt
     9421 tggctgattc gcctatccgc ctcaacttgc aggtagaaga tgaaacccgt actcaggaac
     9481 gtctgcgccg cggcgaagtg gtcggcgcgg tgagtattca acatcaggcg ctgccgagtt
     9541 gtcttgtcga taaacttggt gcgctcgact atctgttcgt cagctcaaaa ccctttgccg
     9601 aaaaatattt ccctaacggc gtaacgcgtt cggcattact gaaagcacct gtggtcgcct
     9661 ttgaccatct tgacgatatg caccaggcct ttttgcagca aaacttcgat ctgcctccag
     9721 gcagcgtgcc ctgccatatc gttaattctt cagaagcgtt cgtgcaactt gctcgccagg
     9781 gcaccacctg ctgtatgatc ccgcacctgc aaatcgagaa agaactagcc agcggtgaac
     9841 tgattgactt aacgccaggg ctatttcaac gccggatgct ctactggcac cgctttgctc
     9901 ctgaaagccg catgatgcgt aaagtcactg atgcgttgct cgattatggt cacaaagtcc
     9961 ttcgtcagga ttaatccatc aaataatgcc tgatagcaca tatcaggcgt tatcctcact
    10021 tctttttgta ttccttgaat cacatcacaa aatagacaaa tctcaggcgg caaaaaacga
    10081 cgtctgaatg catttttttg ctggcgacaa acccacgtaa aaagctcacc gtaagcgcaa
    10141 ataccctcat tttgattgcg ttttacggag caaataatgt ctaacgagca ggagtggcaa
    10201 cagcttgcca acaaggaatt gagccgtcgg gagaaaactg tcgactcgct ggttcagcaa
    10261 accgcggaag ggatcgccat caagcagctg tataccgaag ccgatctcga taatctggag
    10321 gtgacaggta cccttcctgg tttgccgccc tacgttcgtg gcccgcgtgc cactatgtat
    10381 accgcccaac cgtggaccat ccgtcagtat gctggttttt caacagcaaa agagtccaac
    10441 gctttttatc gccgtaacct ggccgccggg caaaaaggtc tttccgttgc gtttgacctt
    10501 gccacccacc gtggctacga ctccgataac ccacgcgtgg cgggtgacgt cggcaaagcg
    10561 ggcgtcgcta tcgacaccgt ggaagatatg aaagtcctgt tcgaccagat cccgctggat
    10621 aaaatgtcgg tttcgatgac catgaatggc gcagtgctac cagtactggc gttttatatc
    10681 gtcgccgcag aagagcaagg tgttacacct gataaactga ccggcaccat tcaaaacgat
    10741 attctcaaag agtacctctg ccgcaacacc tatatttacc caccaaaacc gtcaatgcgc
    10801 attatcgccg acatcatcgc ctggtgttcc ggcaacatgc cgcgatttaa taccatcagt
    10861 atcagcggtt accacatggg ggaagcgggt gccaactgcg tgcagcaggt agcatttacg
    10921 ctcgccgatg ggattgagta catcaaagca gcaatctctg ccggactgaa aattgatgac
    10981 ttcgctcctc gcctgtcgtt cttcttcggt atcggcatgg atctgtttat gaacgtcgcc
    11041 atgttgcgtg cggcacgtta tttatggagc gaagcggtca gtggatttgg cgcacaggat
    11101 ccgaaatcac tggcgctgcg tacccactgc cagacctcag gctggagcct gactgaacag
    11161 gatccgtata acaacgttat ccgcaccacc attgaagcac tggctgcgac gctgggcggt
    11221 actcagtcac tgcataccaa cgcctttgac gaagcgcttg gtttgcctac cgatttctca
    11281 gcacgcattg cccgcaacac ccagatcatc atccaggaag aatcagaact ctgccgcacc
    11341 gtcgatccac tggccggatc ctattacatt gagtcgctga ccgatcaaat cgtcaaacaa
    11401 gccagagcta ttatccaaca gatcgacgaa gccggtggca tggcgaaagc gatcgaagca
    11461 ggtctgccaa aacgaatgat cgaagaggcc tcagcgcgcg aacagtcgct gatcgaccaa
    11521 ggcaagcgtg tcatcgttgg tgtcaacaag tacaaactgg atcacgaaga cgaaaccgat
    11581 gtacttgaga tcgacaacgt gatggtgcgt aacgagcaaa ttgcttcgct ggaacgcatt
    11641 cgcgccaccc gtgatgatgc cgccgtaacc gccgcgttga acgccctgac tcacgccgca
    11701 cagcataacg aaaacctgct ggctgccgct gttaatgccg ctcgcgttcg cgccaccctg
    11761 ggtgaaattt ccgatgcgct ggaagtcgct ttcgaccgtt atctggtgcc aagccagtgt
    11821 gttaccggcg tgattgcgca aagctatcat cagtctgaga aatcggcctc cgagttcgat
    11881 gccattgttg cgcaaacgga gcagttcctt gccgacaatg gtcgtcgccc gcgcattctg
    11941 atcgctaaga tgggccagga tggacacgat cgcggcgcga aagtgatcgc cagcgcctat
    12001 tccgatctcg gtttcgacgt agatttaagc ccgatgttct ctacacctga agagatcgcc
    12061 cgcctggccg tagaaaacga cgttcacgta gtgggcgcat cctcactggc tgccgatcat
    12121 aaaacgctga tcccggaact ggtcgaagcg ctgaaaaaat ggggacgcga agatatctgc
    12181 gtggtcgcgg gtggcgtcat tccgccgcag gattacgcct tcctgcaaga gcgcggcgtg
    12241 gcggcgattt atggtccagg tacacctatg ctcgacagtg tgcgcgacgt actgaatctg
    12301 ataagccagc atcatgatta atgaagccac gctggcagaa agtattcgcc gcttacgtca
    12361 gggtgagcgt gccacgctcg cccaggccat gacgctggtg gaaagccgtc acccacgtca
    12421 tcaggcgcta agtacacagc tgcttgacgc cattatgccg ttctgtggta acgccctgcg
    12481 actgggcgtt accggcacac ccggcgcggg gaaaagtacc tttcttgaag cctttggcat
    12541 gttgttgatt cgtgagggat taaaggttgc tgttattgcg gtcgatccca gcagcccggt
    12601 cactggcggt agcattctcg gggataaaac ccgcatgaat gacctggcgc gtgccgaggc
    12661 ggcgtttatt cgcccggtac cctcctccgg tcatctgggc ggtgccagtc agcgagcacg
    12721 ggaattaatg ctgttatgcg aagcggcggg ttatgacgta gtgattgtcg aaacggttgg
    12781 cgtcgggcag tcggaaacgg aagtcgcccg catggtggac tgttttatct cgttgcaaat
    12841 tgccggtggc ggcgatgatc tgcagggcat taaaaaaggg ctgatggaag tggctgatct
    12901 gctcgttatc aacaaagacg atggcgataa ccataccaat gtcgccattg cccggcatat
    12961 gtacgagagt gccctgcata ttctgcgacg taaatacgac gaatggcagc cacgggttct
    13021 gacttgtagc gcactggaaa aacgtggaat cgatgagatc tggcacgcca tcatcgactt
    13081 caaaaccgcg ctaactgcca gtggtcgttt acaacaagtg cggcaacaac aatcggtgga
    13141 atggctgcgt aagcagaccg aagaagaagt actgaatcac ctgttcgcga atgaagattt
    13201 cgatcgctat taccgccaga cgcttttagc ggtcaaaaac aatacgctct caccgcgcac
    13261 cggcctgcgg cagctcagtg aatttatcca gacgcaatat tttgattaaa ggaattttta
    13321 tgtcttatca gtatgtgaac gttgccacta tcaacaaagt ggcggtcatt gagtttaact
    13381 atggacgaaa acttaatgcc ttaagtaaag tctttattga tgatcttatg caggcgttaa
    13441 gcgatctcaa ccggccggaa attcgctgta tcattttgcg ggcgccaagt ggatccaaag
    13501 tcttctccgc aggtcacgat attcacgaac tgccgtctgg cggtcgcgat ccgctctcct
    13561 atgatgatcc attgcgtcaa atcacccgca tgatccaaaa attcccgaaa ccgatcattt
    13621 cgatggtgga aggtagtgtt tggggtggcg catttgaaat gatcatgagt tccgatctga
    13681 tcatcgccgc cagtacctca accttctcaa tgatgcctgt aaacctcggc gtcccgtata
    13741 acctggtcgg cattcacaac ctgacccgcg acgcgggctt ccacattgtc aaagagctga
    13801 tttttaccgc ttcgccaatc accgcccagc gcgcgttggc tgtcggcatc ctcaaccatg
    13861 ttgtggaagt ggaagaactg gaagatttca ccttacaaat ggcgcaccac atctctgaga
    13921 aagcgccgtt agccattgct gttatcaaag aagagctgcg tgtactgggc gaagcgcaca
    13981 ccatgaactc cgatgaattt gaacgtattc aggggatgcg ccgcgcggtt tatgacagcg
    14041 aagattacca ggaagggatg aacgctttcc tcgaaaaacg taaacctaat ttcgttggtc
    14101 attaatccct gcgaacgaag gagtaaaaat ggaaactcag tggacaagga tgaccgccga
    14161 tgaagcggca gaaattatcc agcataacga catggtggca tttagcggct ttaccccagc
    14221 gggttcgccg aaagccttac cgaccgcgat tgcccgcaga gctaacgaac agcatgaggc
    14281 caaaaagccg tatcaaattc gcctgctgac gggggcgtca atcagcgccg ccgctgacga
    14341 tgtactttct gacgccgatg ctgtttcctg gcgtgcgcca tatcaaacat cgtccggttt
    14401 acgtaaaaag atcaatcagg gcgcggtgag tttcgttgac ctgcatttga gcgaagtggc
    14461 gcaaatggtc aattacggtt tcttcggcga cattgatgtt gccgtcatcg aagcatcggc
    14521 actggcaccg gatggtcgag tctggttaac cagcgggatc ggtaatgcgc cgacatggct
    14581 gctgcgggcg aagaaagtga tcattgaact taatcactat cacgatccgc gcgttgcaga
    14641 actggcggat attgtgattc ctggcgcacc accgcggcgc aatagcgtgt cgatcttcca
    14701 tgcaatggat cgcgtcggta cccgctatgt gcaaatcgat ccgaaaaaga ttgtcgccgt
    14761 cgtggaaacc aacttgcccg acgccggtaa tatgctggat aagcaaaatc ccatgtgcca
    14821 gcagattgcc gataacgtgg tcacgttctt attgcaggaa atggcgcatg ggcgtattcc
    14881 gccggaattt ctgccgctgc aaagtggcgt gggcaatatc aataatgcgg taatggcgcg
    14941 tctgggggaa aacccggaaa ttcctccgtt catgatgtat tcggaagtgc tacaggaatc
    15001 ggtggtgcat ttactggaaa ccggcaaaat cagcggggcc agcgcctcca gcctgacaat
    15061 ctcggccgat tccctgcgca agatttacga caatatggat tactttgcca gccgcattgt
    15121 gttgcgtccg caggagattt ccaataaccc ggaaatcatc cgtcgtctgg gcgtcatcgc
    15181 tctgaacgtc ggcctggagt ttgatattta cgggcatgca aattcaacgc acgtagccgg
    15241 ggtcgatctg atgaacggca tcggcggcag cggtgatttt gaacgcaacg cgtatctgtc
    15301 gatctttatg gctccgtcga ttgctaaaga aggcaagatc tcaaccgtcg tgccaatgtg
    15361 cagccatgtt gatcacagcg aacacagcgt caaagtgatc atcaccgaac aagggatcgc
    15421 cgatctacgc ggtctttccc cgcttcaacg cgcccgcact atcattgata attgtgcaca
    15481 tcctatgtat cgggattatc tgcatcgcta tctggaaaat gcgcccggcg gacatattca
    15541 ccacgatctt agccacgtct tcgacttaca ccgtaattta attgcaaccg gctcgatgct
    15601 gggttaatgg tttgtcccgt cacgatcttc agcaatatgc tgaagcatcg tggcggtata
    15661 acgatgattt ctgattgaat aaagatattc ctgcatatac atcggcctgt gcggcgcatc
    15721 aaaatatttc agcgttgccg gattcaccag tcgccaggca aaatgcggga tcaccgtcag
    15781 aaaatgtccg cgctccacgg cgctgatttt ggccataaaa ctgtagggac gataaataat
    15841 ggtgggattg atcccacaag gtcgcatatt cgcatcaatc attgcttcga agttagcccg
    15901 gttttgaaag cgcatttgta gccagggcag ctcctgaagc agctcttgct cttgacgctc
    15961 ttcataacgg cgagaaacga gaaagcccaa tcgcagcggt ggtaattcac tgatagtcag
    16021 gttttccagt tcctgaactc gtgcagatac atgctgtggg gagataataa aatctaactg
    16081 acggtcaaaa agattatcga taacgccgtt ttcgctgaac tcaactggct gggcggtcac
    16141 gccttcatac ttatctccga gactaatcaa ctgatcgaaa ataattgtcg gataagtatt
    16201 atctatgcca atcacaatt
//
LOCUS       DAZPBM010000089        16084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPBM010000089 DAZPBM010000000
VERSION     DAZPBM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.089"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(167..319)
                     /locus_tag="ACHUZQ_002585"
     CDS             complement(167..319)
                     /locus_tag="ACHUZQ_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1026247.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(584..1318)
                     /gene="cysH"
                     /locus_tag="ACHUZQ_002586"
     CDS             complement(584..1318)
                     /gene="cysH"
                     /locus_tag="ACHUZQ_002586"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1026248.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1393..3105)
                     /gene="cysI"
                     /locus_tag="ACHUZQ_002587"
     CDS             complement(1393..3105)
                     /gene="cysI"
                     /locus_tag="ACHUZQ_002587"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1026249.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3105..4904)
                     /gene="cysJ"
                     /locus_tag="ACHUZQ_002588"
     CDS             complement(3105..4904)
                     /gene="cysJ"
                     /locus_tag="ACHUZQ_002588"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1026250.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5223..5585
                     /gene="queD"
                     /locus_tag="ACHUZQ_002589"
     CDS             5223..5585
                     /gene="queD"
                     /locus_tag="ACHUZQ_002589"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1026251.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5663..6934
                     /locus_tag="ACHUZQ_002590"
     CDS             5663..6934
                     /locus_tag="ACHUZQ_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1026252.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6925..7185
                     /locus_tag="ACHUZQ_002591"
     CDS             6925..7185
                     /locus_tag="ACHUZQ_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1026253.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7202..7777
                     /locus_tag="ACHUZQ_002592"
     CDS             7202..7777
                     /locus_tag="ACHUZQ_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR1026254.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7925..8785)
                     /locus_tag="ACHUZQ_002593"
     CDS             complement(7925..8785)
                     /locus_tag="ACHUZQ_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1026255.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8782..9561)
                     /locus_tag="ACHUZQ_002594"
     CDS             complement(8782..9561)
                     /locus_tag="ACHUZQ_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1026256.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9539..10489)
                     /locus_tag="ACHUZQ_002595"
                     /pseudo
     CDS             complement(9539..10489)
                     /locus_tag="ACHUZQ_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10476..11909)
                     /locus_tag="ACHUZQ_002596"
     CDS             complement(10476..11909)
                     /locus_tag="ACHUZQ_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1026257.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11979..12795)
                     /locus_tag="ACHUZQ_002597"
                     /pseudo
     CDS             complement(11979..12795)
                     /locus_tag="ACHUZQ_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13113..14390
                     /locus_tag="ACHUZQ_002598"
     CDS             13113..14390
                     /locus_tag="ACHUZQ_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR1026258.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14417..15903
                     /locus_tag="ACHUZQ_002599"
                     /pseudo
     CDS             14417..15903
                     /locus_tag="ACHUZQ_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3804 a         4230 c         4040 g         4010 t
ORIGIN      
        1 attcaataaa atatttacat tatgaataat caaataattt tcatccgtaa tatatatgaa
       61 taataaacat aggcataata ttcacataaa taatataaat taaaaaaggg tgcaggagac
      121 caaaccatca caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt
      181 cgtaagcgag aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg
      241 agtcgcctac catcagcgtg aatcccagta ctgtaaaaca cagcactatg attgccacaa
      301 gggcatattt tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt
      361 ttgggttcga acgctggcct caggttgata gaaatatcgc ctggggcttt tgtccatctg
      421 gaacctcgcg aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga
      481 taattcttcc agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc
      541 ccccatcatt tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc
      601 cacattccct ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt
      661 tacgggttgt atgggtatca ccgaccgata aatatccttc atcccataat gggtgatatt
      721 tcaggccatg tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca
      781 gcactttaaa tacgccacgc tgaattgcca gcaccggtaa attggcgcga ctgccggatt
      841 gttcacggcg caggccagca aaccaggttt gcgcgttaag ttctttcaga gcccggttca
      901 tcggttcgac tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgctcccaca
      961 gttttccgta gcgtgcttcc tgccaggctg cgctttcggt agcacggtac actttcaggt
     1021 tgagcttgag tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacaagtaac
     1081 ccgtatcggt gaggatcacc ggaatatccg ggcgaatttg attcaccaga tgcaggctca
     1141 ccgccgcctg aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg
     1201 cccaggctac gcggccttca gcgtccagtt tttccagttc ggcgttagtt tccgccagcg
     1261 ccagaatacg atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag
     1321 attcctcact gtttgccttg cctgatgcga cgctcacgcg ttcttatcag gcctacaaga
     1381 ccgggctgat ggttaatccc acaaatcgcg cgccggatcg agcaccgggc gaatgatgcc
     1441 cgcacgcacc gtaaagtcgc cgaagccttc acccgcttcg cgctctttcg cccagcgccc
     1501 tatcagttca tcaagcgacg ccaggatttc cggctcggtg atgttttctt tatacatccg
     1561 tgggatacgt gtcccaatgc ggttgccgcc aagatgcagg ttgtagcgac ccggcgcttt
     1621 acccaccagg cccacttccg ccagcatcgc gcgaccacaa ccgttcgggc agcctgttac
     1681 acgcatcacg atatgctcat cgctgacacc atgtttcgcc attaaattat cgatgttgtc
     1741 gataaaagac ggcaggaaac gctctgcttc cgccatcgcc agcgggcaag tcgggaatga
     1801 cacgcaggcc atcgagtttt cacgctgcgg cgtgacggca ttcattaacc cgctctcttt
     1861 ggcgatcttc tcgatcttcg ctttctcgct ttccggtaca ccggtgatga tcagattctg
     1921 gttcgccgta atgcggaaat cgcctttgtg gatcttcgcg atctccagca ggccggtttt
     1981 cagcggacgc cccggataat caaggatgcg accattttcg ataaacagcg tcaggtgcca
     2041 gttatcatca atgcccttaa cccagccaat acgatcgcct cgtccggtga actcatatgg
     2101 acggatcggt tcaaatttga tccccgcgcg acgctccact tccgctttaa acgtctcaac
     2161 ccccacgcgc tccagcgtgt atttggtttt ggcattttta cgatcggttc ggttacccca
     2221 gtcacgctga gttgtcacga cggcttcggc caccgccagc gtatgctcca gcggcagata
     2281 gccaaactcg ctcgccgtgc gggcgtaggt tttcttgttg ccgtgttcga tagaaagccc
     2341 accgcccacc agcaggttga agcctaccag cttgccgttt tcggcgattg ccacgaagtt
     2401 catgtcgttg gcatgcagat cgatatcgtt ctgcggcggg atcactaccg tggttttgaa
     2461 cttacgcggc aggtaggtct gaccaaggat cggttcttca tcagtagtgg cgactttttc
     2521 ctggtcgagc cagatctccg catacgcgcg ggtacgaggc aacagatgct cagaaatctt
     2581 cttcgcccac tcgtacgctt ccgcgtgcag ctgcgactcg taagggttcg aggtgcagag
     2641 tacgttacgg ttcatgtcgt tagctgtcgc cagcgcatca agaccgaccg agtgcagcat
     2701 ctggtgcacc ggtttgacgt tctttttcag aatgccgtgg aactgaaacg tctggcggtt
     2761 ggtcaggcga atgctgccat agatggtgtt ttcaccggca aatttgtcga tcgcctgcca
     2821 ctgtttagtg gtaatcaccc cacccggcag acgacagcga agcagcatcg cgtggcgcgg
     2881 ctccagcttc tgttcagcac gttcggcacg gatgtcgcgg tcatcctgct gatacatgcc
     2941 gtggaagcgg atcagcagga agttgtcgcc cttaaagccg ccggtcagac cgtcgtttaa
     3001 atcttccgca atggtgccgc gcaggtagtt gctttcaagc ttcatgcgct cggcgtctgt
     3061 cagttttcct tcgaccacta aaggccctgg atgtttttcg ctcattagta gacatctcgc
     3121 tgataacggc gctctacgcg cagctcactt aaaaattcat ccgccgcttc ggtgtccatg
     3181 ccaccaaatt cggcaatcac ttccagaagt gcctgctcaa cgtctttcgc catgcgatta
     3241 gcgtcgccgc agacataaat gtgggcaccg tcattgatcc agcgccacag ctccgcgccc
     3301 tgttcgcgca gtttgtcttg tacataaact ttttcttttt ggtcgcgcga ccaggcaaga
     3361 tcgatacgcg tcagcacgcc ctctttgacg tagcgctgcc actccacctg gtagaggaaa
     3421 tcttccgtaa agtgcgggtt gccaaagaac agccagtttt tacccggcgc ttcgtcggcg
     3481 gcgcgttgct gcataaaggc acggaacggc gcgatgccgg taccggggcc aatcataatc
     3541 actggcgttt gcgggttagc gggcaggcgg aaattgtcgt tatgttcgat aaatacgcgg
     3601 acttcgccct cttcttccac gcgatccgcg aggaagctgg aggcaccacc ggcacgggcg
     3661 cggccttcca cgtcgtaacg caccacacca acggtgacgt gtacttcgtt ctcgacttcc
     3721 gcctgcgagg aggcgatgga atacaggcgc ggggtcagcg ggcgcagcag gttaatcagc
     3781 gcttcggcat ccagttgcgc cggagagaaa cgcaccatgt cgacaatcgg cgtggtcgcg
     3841 gcgtaatgct gtaacttcgc tttatcgccc accagcggca gcagcgtttc gctgcgggta
     3901 agcgtggcat agttctcaac aatgttggcg gtgttgacgg tcagttcgaa gtgccactgt
     3961 agcgcttcgt tcagaggcaa cgttttgccc tcgacggtga caggttcatc gcctttcagc
     4021 cacagcagtt cgacaagttc tttcaccagt gccgggtcgt tctgatacca gacacccagc
     4081 gcgtcacccg gctggtaacg caggcctgaa tcgcctaagt caatttcgat atgacgaacg
     4141 tctttttcag agttacgccc ggtaattttc tggttaaccg aaaggctcgc caccagcggc
     4201 gcgtctttgc tgtacgggct ggtgtggatt tcatttaccg cgccagtagc gacggattgc
     4261 gaaggtgccg cgacaggcgc acgcgattta agcgcatcaa ccacgcgggc gcgccactcg
     4321 ctggcagcag cctggtattc aacatcggca tcgacacggt cgagcaggcg ttcaccaccc
     4381 agttccgcca gcttgctgtc gaaatctttc ccggactggc agaaaaattc ataagagcta
     4441 tcgccgaggc taaacacggc aaacgcggtg ttttccagct ttggcgcttt tttggagaac
     4501 aggaacttat gcagcgcgac ggcttcttcc ggcggttccc cttccccttg cgttgacgtc
     4561 actacgatga gcagtttttc gctggcgatt tgtttgaatt tatagtcgcc cgcgttcacc
     4621 agcttaacgt tcagttttgc tgctaataaa tcatcgcgta atgcttcagc aacccggcgc
     4681 gcattgccgg tttgcgaggc ggagataata gttatacccg gcatttctgc ggctggcgct
     4741 ggcgtcgctg caagcgcagc aggctgctga ttgagtacgc cccagaaata gccagaaacc
     4801 caggcaagct gggtaggcgt taaatcggtc gtggccgcct gaaggcgtgc cagttgctcc
     4861 gggttcaacg gaagcaacgc ggaaggtggg acctgtgtcg tcatgcgtcg ttatgttcca
     4921 gtaagcaaag ctgtttctgc gccctgtcag cgcccataaa acagaagaga agataaggtt
     4981 aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc aaatcgacta
     5041 acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat gaaaaacaaa
     5101 tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc gttttagata
     5161 atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt agagaaatta
     5221 tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc ttaccacacg
     5281 tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg cgactggaaa
     5341 ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa ctgaaagcgg
     5401 cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt ccaggtctgg
     5461 aaaacccaac cagcgaagtt ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc
     5521 cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc agggtgtatc tatcgcggcg
     5581 aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta gggtgttgtg
     5641 ttctggatat ctggggcatg acatggaaga cgactgcgac attattatta ttggtgccgg
     5701 tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg ttttgttact
     5761 ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc gggcggttat atacccatgc
     5821 actcgcggaa ctcctcccgc aatttcatct gaccgcgcct cttgaacgac gcatcactca
     5881 cgaaagcctt tccctgttaa cgcccgatgg cgtaacgacg ttttccagct tacagcccgg
     5941 cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg ccgaagccga
     6001 aaaagaaggt gtcgaatgca tccccggagc gacggtggat gcactgtatg aagaaaacgg
     6061 cagagtctgt ggcgttattt gtggtgacga tattctccgc gcccgttatg tggtgctggc
     6121 agaaggtgcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc ctgctggcga
     6181 agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta ttgaagaacg
     6241 ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggaa tctgtgatga
     6301 cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag ggattgtttg
     6361 cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctga ctcgctttaa
     6421 agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt atggtgcgca
     6481 tctggagcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta acggctggct
     6541 gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc gcggcatgga
     6601 tatggcgctg actggcgcac aggcggcggc acaaacgcta ataagcgcct gccagcaccg
     6661 cgagccgcaa aatctgtttg cgctttatca tcacaacgta gagcgcagcc tgctatggga
     6721 tgttctacaa cgttatcagc atgttccggc gcttttgcaa cgccctggct ggtatcgggc
     6781 gtggcctgcg ttaatgcagg atatttcccg cgatttatgg gatcagggtg ataaacctgt
     6841 tccaccgctg cgccagttat tctggcgtca tttacgtcgt catggcctgt ggcatctggc
     6901 gggcgatgtt atcaggagtc tgcgatgtct gtagcccata atctctggcg cgttgctgat
     6961 gcgccgcaca ttgttccggc tgactccgtt gagcgtcaga tggtacaacg gttgattaac
     7021 gcctgtccgg caggtctttt ttcgctcaca ccggaaggtg acttacgcgt tgattatcac
     7081 ggctgcctgg agtgtggcac ctgccgtttg ctgtgcgacg aatccacact acaacagtgg
     7141 cgctatccgc cttccggatt cggcatcacc taccgctttg gataaataat aaggataatt
     7201 tatgcccctc ttacacctac tccgccagaa tccggtgatt gctgccgtta aagataatgc
     7261 cagcctgcaa ctggctatcg attctgaatg ccagtttatt tccgtgttgt acggcaatat
     7321 ctgcaccatc agtaatatcg ttaaaaagat taagaacgcc gggaaatatg cttttattca
     7381 tgttgatttg ctggaaggcg cgtcaaataa agaagtcgtt attcagtttt tgaaactggt
     7441 gaccgaggcg gacggcatta tcagtaccaa agcctcaatg ttgaaagcgg caagagcgga
     7501 aggttttttc tgtattcatc gcctgtttat tgttgattca atttcgtttc acaacattga
     7561 taagcaagtt gcgcaatcga atccggattg tattgagatc ctgccaggct gtatgcccaa
     7621 agtgctgggc tgggtgacag agaaaatccg ccaaccgctg attgccggtg ggctggtgtg
     7681 cgatgaagaa gatgcgcgta atgcgattaa cgcgggtgtc gtggcgcttt ccaccacgaa
     7741 taccggggtc tggacgttag cgaaaaaatt actttgacgg gataatcgta accaattgaa
     7801 tttggtttga tttgtaggcc gcacgccaca tccgacattc agcgcctgat gcgacgcttg
     7861 acgcgtctta tcaggcctac aagtccccag gccagaaccg taggcaggat catccggcaa
     7921 cacgttattg gcaatctgcg tgaatgtttg tcaccagtgc ctccagcacc actttccagt
     7981 catcaaccac gccaacatct gcctgtgaaa ataccgcagc gctggcgtca tgattaatcg
     8041 ccaccacaaa cttgctattg cgtacgcccg ccattaacgc tgctgcgcca gaagcgccaa
     8101 caacaataca cacctcaggt gccagcagat gtccggaaat accaatcact ttttcagcat
     8161 caacgccacc gttcatcact cgcgcccgac tgtagcccac ttcggctccc agcttttctg
     8221 ccagcatagc gatttcctgg ttatctgcct ctccgccctg ccccaccacc agcacccgtc
     8281 tggcctcagc taatgggtcg cgagtaacat ttttcagatc ctctgtgcta accagccagt
     8341 ccggaagcgc acccgggaca atgttcagtt gttgcattcc agagggcaac gtggcgtttt
     8401 tcgctgctcc cgcctgacgc gccagggaaa ggcatagcgg gcgtttttct gtttgcagcg
     8461 tcgccgttag cgcatttccc cagtgggatt tgcgcacgct tacggttgga atatctaacg
     8521 aagtcacctg gcagatgctt gcaccatgta aacgccaggc cagccgggtc gacagttcat
     8581 cgccaaacgt tcccggcgga aagagaacaa cgtccgcagg cgttcgctgc cactgttcca
     8641 ccaacgcatc cagcacctgt tccgccacta caggctgagg ttcaatctgc cagtgcgcca
     8701 gggtacaacc gctgaaatcc tgcgccgcca gccagcttgc tattgcggca ttctcctgat
     8761 ttattgtcac aattgcgata ttcatggctg catcctttgg cgtaaatagt cctgccatag
     8821 cttttgagct ttttctgcta ccgtttgccc atcaatcagc gtcgctccgc gtcgttgctc
     8881 tgctcttgcc agttgcagac actgcatcgc tggcatttcg gcagcaacag ttttacggat
     8941 aatttctgct ttccccgcag ccattcgctg gcgcattccc ggtactggca aggccacttc
     9001 accgcactga cgcacggcaa tcaccgcagg caagcgaacc cggcaacaac gcaacccatg
     9061 ttcagtacgc tgttcgaggg tgataaacag cgcgtcgaga gtgaaacgct ccacctgggt
     9121 gaagcagggc cagcccagca tttccgccag caaaaatggc gtttgcccat tctgcccttc
     9181 gctgctttgg cagccggtaa ttatcagatc cagcggattc tgatgctgcc attcggcaat
     9241 atggcgagcg acaaattccg gcgcaaagcg cagatctgcc gccgtctcca gcaatacagc
     9301 ttcctcaaac ccaagagcca tgagatagcg cagccagtgc aatgcccgtt catcccccat
     9361 gctcaacgcg gttaaagaca tcggcgtacc gttttttctc tgcgccaaca gcagcgcggc
     9421 agcggcctgt tcatcagcac cgagtaaact tcgcagtagc gaaatatccg gtccgctttt
     9481 accctgagcc gccgcctgcc actctttttc cgccagcatt ccggcatccg gttcggcttt
     9541 aaacgctaac agaatgttca ttcgcacctc ctacatttcc cgccgccacc agcgggaggg
     9601 ctttagtttc tggtgcccat agccaggtca caacaaaatc aaccaacaac actgtcgcca
     9661 ggagcaataa ggtgacttgc attccccact gcgccagcac ccacggcagc aggccagtac
     9721 ttaccgccgc gccaagtcga ctcatggcgg tggcaaaacc gacgcccagc gagcgaatgt
     9781 cagtaggaaa actttccgca ggcaaaatgc ccaccagatt actgactgcc gaaatggtgg
     9841 tgctgaagag aacaaaaagc agcagcgtta atgaactgcc ggaaggcaaa caggccatca
     9901 ctaccagcgt tgccgccagc agcaaaaaac ttcccagcaa aaatttgcga tgtgccagca
     9961 ggtgcgtcag aactaatccc agcagcgcgc ccacaattaa caacgcatta agcatcaggc
    10021 tggcagtcag cgcatcttcc agaccaatag tctgggcgat agttggcagc caggtataaa
    10081 tcacaaacca tgggattacg aggcagacaa agaagacgct gttaaacgcc gtgcgccgcc
    10141 agtaacgcga agagaacaag gttttgatgt gtttatgggt cgccgttacc acttcatcgc
    10201 ccagtaaaac atggggacca aaatagcgat gcacgatagc gtgagcttct gcaaaacgcc
    10261 cctggcgcag tagccagcgt ggtgattctg gcgttcccca gcgtaataac gtaatcaaca
    10321 acgcgggcag agctgccgat gccagtagcc agcgccaggc ctccgggttt tcggaaataa
    10381 agtgatgtcc ggcaatactt gccagcacat agcctacggt ccacaccacg ctgaatgcgc
    10441 ccagcaaaat accgcgatgg cggcgcgggg aaaattcagc cagcaaggtt aatgccatta
    10501 ggatcgaact gctttttcag cccttccagc aacgcccacg cgctgccgtg ttccagtttg
    10561 ctccagtgaa cgcgatgttt accgataccg tggtggtgca ccatcgaacc gccgaggcga
    10621 atggtttctt cacagatgat cttgttgagc ggattgtggt acttgtcgat ttcctcttcc
    10681 ggcttacagt caacgacgtt gtaatcgtag acgaagtaca tgttggtgcc gttctgatag
    10741 ctatgagagg aatgaccgcc cagcatggtg atgtcgtcgg cgtgcgggaa ctcagtacga
    10801 atacggttaa taacgctttc gtagatttcg tggatgcagc tccagcagcc ggacacttca
    10861 gtggtaaagc ccatgttgcc ggttttgagg atctgcacac gttcggcagc cactttatcc
    10921 ggtccccagt tcaggttgtt aaaccaggtt tcgatcagct tgctgtccac gcgctggcat
    10981 tgcgggtagc gggcaacgat ttccgcaatc ccttcgcccg tcgccttcgc aatgcgaggg
    11041 ttaccttcag ccataaagat cagcacgcat tttccgtctg caaaatgggt gaagtgttgg
    11101 gtgccatctt cagcgtcata caaacgagcg atcgacggac gatacccttc caccatgact
    11161 tcacgcagga tgttgaagtc ggttttcatg tcttccagga tatagccgta gaagaggttg
    11221 ttttccgggg tgaatttaaa gattttcact gttacttcag tgatatagca aagtgcacct
    11281 tcgttaccga tgatgatgtg gcgaatgtcc gggccagccg cgcggcgtgg cacgttttta
    11341 atgcgtgtga cggtgccatc agccaatact gcttccagac caacgaccat atcttcgatt
    11401 gcgccgtaga gtgtggagaa ctgcccgata ctgcgggttg ctaccaagcc gcccatctgc
    11461 gccagcggct ttgactgcgg agaatgcccc gtggtgtaac ctttttcacg caacgcattt
    11521 tccagcacct ccagcggaac gccacattgc gccgtcgcct gcatattctc aatatcaata
    11581 ttaatgattt gattcatggc ggagccgtca agcaccaccg agttttccac aacggtttcc
    11641 agcccacctt cagtggcgga agcgccggta cgcggcacac cgttaatttt gtgcgcattc
    11701 ataaaattca gcacacggga tacttgctct gtggaaccga gttttacgac cgctgccgga
    11761 atcggcaaag tataaatgcc atgaatatcc ggaaatttac gaaaacggtc aatactgttc
    11821 ttctttaata ctgtttcatc ggtaattacg cgatctgcac caacaatttc ctttagttgg
    11881 tcgacaatcg ctgcgcgaga taaagacata gtaattcctt cctgataata aaaatggaat
    11941 aatcattaat aagagaaaac agaaaccacc gttatggatt agcgcactaa ataaccacca
    12001 ttgaccacta ataaatgccc attgacataa tttgaagccg gacttgcgag gaatacagct
    12061 gcgcccatta aatcctgagt atcgccccaa cggtttgccg gaatatgatc aagaacgcgc
    12121 tgattggttt ctggattact gcgtgtcgcc agcgtaatat ctgttgcata atagccagga
    12181 gcaataccat ttacctgaat attatattga cctagttcat cacaataagc tttggtgaat
    12241 ccggcaagag catgtttagt ggcagaatat gcaggtgacc attgtccacc taagtaagag
    12301 aacaatgaac agatattaat aattttgccg cttttctgcg ggatcataat ttttgcagct
    12361 tcatagctta actcgaatgc ggcggtcagg ttcacatcaa tcatcggatc ccagtcggca
    12421 cgaccgaagt ccagcacctt attcagctta caaataccgg cattgtttac cagaatatca
    12481 actgtaccga aacgctcaca gcaagcagcg ataatcttct gcggcgcgcc ttctgcggtg
    12541 atatccacct gcatgaagtc cacctcaaca ccctgttttt caaacatttc ctttgtttcg
    12601 ccgttatctt tcaatcattt cctttgtttc gccgttatct ttgacgaaac taggaataaa
    12661 gatatttgcg ccagctttgg ccaacgccat ggcaaatgcc tggcctaaac cgctattccc
    12721 accggtaaca attgcggttt tacctttcag tgagaaaaaa tccattgaga acgcattgag
    12781 agattcgatt gacatagtta gctccaattt ccgtaaggca aaaaaaagaa aggtaacttc
    12841 ccccgcgaga gggaagttac ctttctcatt atctctggta actggagata atttttagca
    12901 taacggcata actgctaatg tgatcgtaat cacagtgtga tattcgtatt gtcaaatagt
    12961 tttaaaaagc agaagcgtga ttactctcac acaacacctc tgctttccat gttatttctt
    13021 attgcaatta ctagagacct gagaaaagta atccacctga aagggcggct tacttttctc
    13081 ttttttttgt ctattacaaa tgggaatacg atatgcaaca caactcatat cgccgttgga
    13141 taaccctcgc gataattagt tttagcggcg gcgttagttt tgacctggct tatttacgtt
    13201 atatttatca aattcccatg gcgaaattta tgggattcag caataccgag ataggtttaa
    13261 taatgagtac ctttggtatt gcggctatta ttctttatgc ccccagcggc gttattgccg
    13321 ataaattttc acaccgcaaa atgattactt ccgcgatgat cattaccgga ttactgggtc
    13381 tgttaatggc aacgtatcca ccgctgtggg taatgctctg tattcaggtc gcctttgcga
    13441 taacgacgat tttaatgctg tggtcggtgt cgattaaagc cgcctcgttg ctgggcgatc
    13501 atagtgagca agggaaaatt atgggctgga tggaagggct gcgcggcgtc ggtgtaatgt
    13561 cgctggcggt gtttaccatg tgggtctttt ctcgctttgc accggatgac agcgccagcc
    13621 tgaaaacagt cattatcatc tacagtgtgg tttacatctt gttggggatt ctgtgctggt
    13681 tttttgttag cgataacaac aacctgcgca gtgccaataa cgaagaaaaa cagtcattcc
    13741 agcttagcga catccttgcc gttttgcgta tcagcaccac ctggtattgc agcatggtga
    13801 tttttggtgt cttcacaatc tacgccattc tgagttactc caccaactat ctgaccgaaa
    13861 tgtacggcat gtcgctggtg gcagcgagct acatggggat tgtgatcaac aaaatcttcc
    13921 gcgcgctgtg cggcccactt ggaggcatta tcaccaccta cagtaaagtg aaatccccta
    13981 cccgcgtgat tcaaatcctt tccatcatcg gtttgctggc gttgacagcc ctgctcgtca
    14041 cgaactctaa cccccaatcg gtcgcgatgg ggattggcct gattttactg ctgggattca
    14101 cctgttacgc ctcacgcggg ctgtactggg cctgccccgg cgaagcgaga acaccgtctt
    14161 acattatggg caccacggta ggtatttgtt cggtgattgg attcctgccg gatgtcttcg
    14221 tttacccgat tatcggccac tggcaagaca ccctgcccgc tgcagaagcc taccgcaata
    14281 tgtggctgat gggcatggcg gcgctcggca tggtgattgt ctttaccttt ttgctgttcc
    14341 aaaaaattcg tactgctgat agcgcccccg caatggctag cagcaagtaa gccaggaaaa
    14401 ttacaggaga aaaatcatgt cgaaaaaata catcataggg attgatggcg gaagtcagag
    14461 cacaaaagtg gtgatgtacg atctggaagg taatgtggtt tgcgaaggta aaggcttatt
    14521 acagccgatg cacacgcctg atgccgatac tgcagaacat cctgacgacg atttatgggc
    14581 atcattatgt tttgcaggtc acgatttgat gagccagttt gccgggaata aagaagatat
    14641 tgttggcatc ggcctgggct ccatccgttg ctgccgtgcg ttattgaaag ccgacggcac
    14701 gccagctgcg ccgttgatta gctggcagga tgcacgcgtt acacgccctt acgaacatac
    14761 gaatcctgac gtggcgtatg taacctcttt ttcgggttat ctgacgcatc gcttaaccgg
    14821 cgaatttaaa gataatatcg ccaactattt tggtcagtgg ccggtggatt ataagacctg
    14881 ggcatggagc gaagatgccg cagtcatgga gaagtttaat atcccccgtc atatgctgtt
    14941 tgatgtgcaa atgccaggca ctgttcttgg gcatatcaca cctcaagccg cgctggcgac
    15001 gcatttcccg gcaggactgc cggttgtttg taccaccagt gataaaccgg tagaagctct
    15061 gggagccgga ttactggatg atgaaactgc ggtaatttct cttggcactt acatcgcatt
    15121 gatgatgaac ggcaaagcct tgccgaaaga tccggtggcg tactggccga ttatgtcttc
    15181 tattccgcaa acattgctgt atgaaggtta cggtattcgc aaaggcatgt ggacggtgag
    15241 ctggctgcgc gacatgttag gcgagtcgtt aattcaggat gccagggcgc aggatctttc
    15301 accggaagat ttactcaaca agaaagcctc tagtgtgcca cctggctgta atgggctgat
    15361 gacggtgctg gactggctga ccaatccgtg ggaaccgtac aaacgcggga ttatgatcgg
    15421 ctttgattcc agcatggatt acgcatggat atatcgttcg atattggaaa gcgtggcgct
    15481 gacgctgaag aacaattacg acaatatgtg taatgaaatg aatcactttg cgaaacatgt
    15541 aatcattact ggcggcggtt cgaacagcga tctgtttatg cagatttttg ccgacgtgtt
    15601 caaccttccg gcacgccgta acgccattaa cggttgtgca agcctggggg cagcgattaa
    15661 tacaggggta ggtctggggc tatacccgga ttacgcaacg gctgtcgata aaatggttcg
    15721 cgtgaaagat atctttatac cgattgagag caatgccaaa cgctacgacg cgatgaataa
    15781 aggcattttc aaagacctaa ccaaacatac tgatgtgatc ctgaaaaaat cgtatgaagt
    15841 gatgcatggg gaattgggga attggggaat gcggattcga tccagagctg gtcgaatgcg
    15901 tgaataaggt aggccgggcg gaaaactgcc cggcctgaac atacctgaat ggttatcccc
    15961 gctggcgcgg ggaacataag ttcgacaact taaaaaatga taacaagtcc ggtttatccc
    16021 cgctgatgca gggaacataa taaaaacttt aactagttcg gtttatccct gtttttacaa
    16081 ggaa
//
LOCUS       DAZPBM010000090        16048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPBM010000090 DAZPBM010000000
VERSION     DAZPBM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.090"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHUZQ_002600"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHUZQ_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1026259.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="ACHUZQ_002601"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="ACHUZQ_002601"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1026260.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="ACHUZQ_002602"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="ACHUZQ_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1026261.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="ACHUZQ_002603"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="ACHUZQ_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1026262.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="ACHUZQ_002604"
     CDS             6397..6507
                     /locus_tag="ACHUZQ_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1026263.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHUZQ_002605"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHUZQ_002605"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1026264.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHUZQ_002606"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHUZQ_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1026265.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8887..9053)
                     /locus_tag="ACHUZQ_002607"
                     /pseudo
     CDS             complement(8887..9053)
                     /locus_tag="ACHUZQ_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9350..9982
                     /gene="yihA"
                     /locus_tag="ACHUZQ_002608"
     CDS             9350..9982
                     /gene="yihA"
                     /locus_tag="ACHUZQ_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1026266.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHUZQ_002609"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHUZQ_002609"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1026267.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14446
                     /locus_tag="ACHUZQ_002610"
     CDS             13514..14446
                     /locus_tag="ACHUZQ_002610"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1026268.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14487..15917)
                     /locus_tag="ACHUZQ_002611"
     CDS             complement(14487..15917)
                     /locus_tag="ACHUZQ_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1026269.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3803 a         4097 c         3942 g         4206 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgct
    14221 gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg
    14281 gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa
    14341 tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga
    14401 ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt tactcgcctc
    14461 tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat
    14521 tcttcttcgc tcatctcctg tccgttaaac acaactttac ccggtgtata acgcagctgc
    14581 agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa tgtcatcatc
    14641 ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa
    14701 ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc
    14761 aatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc
    14821 agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc
    14881 tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga
    14941 gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat agcaatgtta
    15001 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
    15061 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
    15121 acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac gaaagcatcg
    15181 atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt
    15241 tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt
    15301 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
    15361 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
    15421 aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
    15481 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
    15541 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
    15601 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg
    15661 cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacga
    15721 gaattaaaaa aacctttgtc aaaattttta taacttaacg tcatatcgta ggcatgttca
    15781 cctttctttt tagcatcgtt aaaatcttta ataaactttt cgacaccagg ctgaatttgc
    15841 gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat aacacctgta
    15901 gctgacttac gtatcatata aatattccct gataaaacag caaaacttta aatatccgct
    15961 tatgctgaaa taatgtccag cggcaggacg cattatcaga atgtatttat atttattaaa
    16021 taataaaaaa aaccgtgaat attcacgg
//
LOCUS       DAZPBM010000091        15963 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPBM010000091 DAZPBM010000000
VERSION     DAZPBM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.091"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUZQ_002612"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUZQ_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1026270.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUZQ_002613"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUZQ_002613"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1026271.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUZQ_002614"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUZQ_002614"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR1026272.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUZQ_002615"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUZQ_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUZQ_002616"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUZQ_002616"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1026273.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUZQ_002617"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUZQ_002617"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR1026274.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUZQ_002618"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUZQ_002618"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1026275.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHUZQ_002619"
     CDS             8229..8939
                     /locus_tag="ACHUZQ_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1026276.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUZQ_002620"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUZQ_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1026277.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHUZQ_002621"
     CDS             9636..10343
                     /locus_tag="ACHUZQ_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1026278.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHUZQ_002622"
     CDS             10343..10891
                     /locus_tag="ACHUZQ_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1026279.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHUZQ_002623"
     CDS             10901..12067
                     /locus_tag="ACHUZQ_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1026280.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHUZQ_002624"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHUZQ_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHUZQ_002625"
     CDS             13576..14229
                     /locus_tag="ACHUZQ_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1026281.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUZQ_002626"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUZQ_002626"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR1026282.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15963)
                     /locus_tag="ACHUZQ_002627"
     CDS             complement(15612..>15963)
                     /locus_tag="ACHUZQ_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1026283.1"
                     /translation="LFYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAA
                     KHQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTR
                     VWSGYLTLKSLQRQ"
BASE COUNT         3700 a         4344 c         4165 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctagatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 aga
//
LOCUS       DAZPBM010000092        15547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPBM010000092 DAZPBM010000000
VERSION     DAZPBM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.092"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(60..254)
                     /locus_tag="ACHUZQ_002628"
                     /pseudo
     CDS             complement(60..254)
                     /locus_tag="ACHUZQ_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(497..1546)
                     /gene="yahK"
                     /locus_tag="ACHUZQ_002629"
     CDS             complement(497..1546)
                     /gene="yahK"
                     /locus_tag="ACHUZQ_002629"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR1026284.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(1633..1707)
                     /locus_tag="ACHUZQ_002630"
                     /pseudo
     CDS             complement(1633..1707)
                     /locus_tag="ACHUZQ_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1923..3305)
                     /locus_tag="ACHUZQ_002631"
     CDS             complement(1923..3305)
                     /locus_tag="ACHUZQ_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1026285.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(3315..4265)
                     /locus_tag="ACHUZQ_002632"
     CDS             complement(3315..4265)
                     /locus_tag="ACHUZQ_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR1026286.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(4408..5826)
                     /locus_tag="ACHUZQ_002633"
     CDS             complement(4408..5826)
                     /locus_tag="ACHUZQ_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1026287.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(5826..7373)
                     /gene="fdrA"
                     /locus_tag="ACHUZQ_002634"
     CDS             complement(5826..7373)
                     /gene="fdrA"
                     /locus_tag="ACHUZQ_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR1026288.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(7363..8226)
                     /locus_tag="ACHUZQ_002635"
     CDS             complement(7363..8226)
                     /locus_tag="ACHUZQ_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1026289.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8266..8871)
                     /locus_tag="ACHUZQ_002636"
     CDS             complement(8266..8871)
                     /locus_tag="ACHUZQ_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR1026290.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9129..9626
                     /locus_tag="ACHUZQ_002637"
     CDS             9129..9626
                     /locus_tag="ACHUZQ_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1026291.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            9718..10650
                     /locus_tag="ACHUZQ_002638"
     CDS             9718..10650
                     /locus_tag="ACHUZQ_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1026292.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(10692..11780)
                     /locus_tag="ACHUZQ_002639"
     CDS             complement(10692..11780)
                     /locus_tag="ACHUZQ_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1026293.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(11922..>15547)
                     /gene="ehaA"
                     /locus_tag="ACHUZQ_002640"
     CDS             complement(11922..>15547)
                     /gene="ehaA"
                     /locus_tag="ACHUZQ_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR1026294.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         3556 a         4233 c         3715 g         4043 t
ORIGIN      
        1 ttaagactat cacttattta agtgatactg gttgtctgga gattcagggg gccagtctag
       61 tcgtcatcga ctaaatcatc acgacaatca taaggataat cataatattg tttaatcaaa
      121 accttgaata tatcgacaca ggaaaccagg gagcttttga tatcctctgg taactgattg
      181 tttttaaccg tattaagtat gctttgttgc gtatatggca tcaaattatt gtgcggagct
      241 tttaatgata tcatcgcgat ctctcttaaa taaaaataaa cgtcgcagat aataaagcgc
      301 tgattaaacc aggcactatc agaaatcgct cataactaac gtgagaagta ttgtgtactg
      361 gagggagtta atttaacaat ttgaatttac gttataggtt ttactgctac tttgatatta
      421 tttcgctctc cttaactaaa aagttaagtg gagagcgaag ttaatatgtt aaaccacagc
      481 gtatttatta attttttcaa tctgttagtg tgcgattatc gataacaaaa cgatatttca
      541 catcaccacg cagcattcgc tcataggctt cattaatttg atcggcccga atcatctcta
      601 tatcagccac gatgccatgt tcggcgcaaa aatcgagcat ctcctgagtt tctggaatgc
      661 cgccaatcat agaatcggct atcgcacggc gtttcatgat caggttgaaa acttccggcg
      721 atttatgcgg tgtcgcaggc gcaccaacca gcgtcatggt gccatcacgc ttcagcaagg
      781 tggtaaaatc gtcgagatta tgtggcgcag ctactgtatt caaaatgaaa tcgaaactct
      841 tcagatgagc cgccatctca tcggcattgc gtgagttaac aacttcatcg gcccccaggg
      901 ctttttccgc ttcgcgtttt gcctcagaag tggtaaatgc caccacatgt gcccccatcg
      961 cgtgggccag cttaatcccc atatgtccca gaccgccgat gccgaccacg cccacttttt
     1021 tacccggccc ggcctgccag tgacgtagcg gcgagtacgt ggtgatccct gcacacaaca
     1081 aaggagccac cgccgccagc tgctcttgcg ggtgacgaat acgcagaaca tatcgctcat
     1141 gaacgacgat ctgttgtgag tagccgccca gagtatggcc cggttcgtcc ggcgtcggcg
     1201 agttataggt gccgatcatg tgatcacagt agttttccag cccgtcctca cactcttcgc
     1261 aatgtttaca actgtcgaca atgcagccga caccgaccag atcgcccggc gcatgttttt
     1321 ctacctgatc accaacggct accacacgcc ccacaatttc atgacccggt acgcaggggt
     1381 aaaccgtccc cgcccactcg gaacggacct ggtggagatc ggaatggcag acgccacagt
     1441 aagcgatttc gattttgaca tcattcggtc ccggttcacg ccgtgtgata tccatcggtt
     1501 caagtggttg tttagcggaa tatgcaccaa cagctttgat cttcattgtg tttactcctg
     1561 attagctatg tgtatttggc gacgcctgat atgtcatcgc ggcaaaacgc gtccattgaa
     1621 tatagccaat atttaaacca cccgatccag ccccaacatg ccgcgaacct gaatgaaaca
     1681 gagcagcaac gttccccata aggcgagcgc ctataagtgt gatatttgct atccgtaaaa
     1741 gactaaaatc ggcaaaactg atgatctttt cacgtagaca aatagttatc tacacaacgt
     1801 caataagggc atattcctca aagataatgt ccatttctgc cgacactatc atattgaggt
     1861 cgccccactc cctggggctt aaatctctta caattcaccg ttgcaggcgt ttcaaatgcg
     1921 catcaacctg ccacactccc ccacaccagt tgccctttat ggaacgttgc ggttctcggc
     1981 gagatacgcg ccaccgcctc ggcggaacag gaggcgtcca ccagcacaaa gctggcatca
     2041 tcctgcgctt ttggccatac acgctcgcct ttttcattca gcggcaatac atcgccagtg
     2101 gctagaaaca gcgaacggga gaggttctgt tcgttaggac gaatatagag ctgcgcgtag
     2161 agattggctt tttccagcat gtcgcccaga ccataaggcg accagtggtc gataacgctg
     2221 tcagtgccag tcatcacttt tacgcctttg tcgtgcaact gtttgagcgg catatgcagc
     2281 gtgccaatcg gcaccgtcga ggcgatagaa atttgctgcg ccgccatccg attcgccagc
     2341 gcatcgacct gttgttcgtt gagcgtagcc agcgcaaagg cgtggctgat ggtcagctta
     2401 cctttcagtt gcggcgtttt ctctaccgtt tcaaccatat aattgatggc tgccacgccc
     2461 gacggagtgg tttcgtgcag gtgaatatcg acacctttgt cgtagtccag cgcaatctga
     2521 aacatggtgt cgagggattt ttccatcgcg ccatcgacac tggtcgggtc cagcccgccg
     2581 acgtaatgcg cacccgcctg catcgcttcg cgcattaagg gttccgattt cgacagcagc
     2641 aaaccgtgct gcgggaaggc gacgatttca cactcgaagc ccgcctgacg ccgcgccagc
     2701 accgcctgca aattttgcag attttttagg ccggaaaccg gttcgatatt gcagtggctg
     2761 cgggcaatgg tggtgccttt cgactgcaat agatcgatca gtttttccgc ccgctcctgc
     2821 gtatacggtt gtaactccgg cagcattttc tgcttgagtt tgatcatgtc ctggatagtg
     2881 gtgcctgccg gacgattgag cgagcgccac ggcccgccgt agaaggtttt gtcgagatga
     2941 atatgcatgt cgcgagtggt gggtagcatc aacttaccgc cagcgtcata gtgcggcagc
     3001 gtggcgtccg gatgctgctt gttctcgcgc agggcgacaa ttttgccgtc ctgaatctcc
     3061 acggtctggc gcgccgtgcg ggtctgcacc gccacaccgt tttcgtagtc aaaaccggtt
     3121 tccagcagca cgttatcgag atagtaatgc gggtcagtga ttttcatggt gttgttcgct
     3181 tcgcagttta gggtgccagc taccgccgca tgggcgagcg gcacataggt accaaacagc
     3241 gcggcggcgg tgaccatctt accgctctgg ctcagaaatt cacggcggct attgctttct
     3301 ttcatcctac gtccttacgt tttaataata tgggtgcccg tttcaccgcg cagcgccgca
     3361 ggcaggcatt ccggcgtggt gataatcact tctttgccgc cctgctccag gaacgtcagg
     3421 ctggcgatga tttttggcaa catgctgccg ggagaaaaat gcccttcctg catatagcga
     3481 gtcatggtgg caatatccac ccgatccagc gcctgctgct gcggtttacc aaagtggata
     3541 cagacttttt cgacgtcggt ggtgatcacc agaatgtcgg cgtgaatttc acgggccagc
     3601 agcgcggtag agagatcttt gtcaataacc gcgtccacgc tttggtaatc ccccgcttca
     3661 gtacgcacta ccggaattcc accgccgccc gcgccaatta cgacaaaacc ttgttggatc
     3721 agcgccttaa tggcgggtgc ttcgacaata cgtttcggtt ccggcgaggc gaccacgcga
     3781 cgatagcccc gtccggcatc ttcaacaaaa caccagtcag ggtttgcctt ttgtagtttg
     3841 tcacgctggc tttcactgaa gaatgcacca atgggcttgg tgggatgggc aaaacctgga
     3901 tcgtttttat ccacttccac ctgagttacc acggtgacgg cttttttctc gccgtgacgc
     3961 gccagtcggt tattcagcgc ctgttggatc agatagccga tgccgccctg cgtatctgcc
     4021 acacagttcg ccagcggcgt taagggcagc ccttcgcgct cgtgggcaat ctccgcacgg
     4081 cgtaaatcca gcccgacctg cggcccgtta ccgtgggtca gcacaatgtc gtaatcggaa
     4141 gccagcattt ccagcaccgt atcggccacg gctttcaccg cctccgcctg atgctcaatc
     4201 gactggctgg cgttatcttt gataatgctg ttgccaccaa tggcaacgac cacaagctct
     4261 ttcatgttgt tatgtccgct acagagggtg atgaggttat gaccaacgcc agactgcctg
     4321 atgcgctacg cttatcaggc ctacaattct cgggccatcc gtaggccgga taaggcgttc
     4381 acgccgcatc cggcataaac atgtaagtca gacgccgtaa cgttcgcacc agccgagaat
     4441 ggctttctcg aagcaagcga atggtggatg cacaatgccc gcgccaatca tgccgacgcc
     4501 cgcatcttta tgggcgatgg cggtgttgat caccggcaga atgccgctgc tacccacgcg
     4561 ggtgatgtcg attgccgacg gcacgcccat aaaaccgagc agcggaatgg tgacgttggg
     4621 gttttcaccg agagtgattt cacgcatctg acgggagaaa tcgatagcct cttccaccgt
     4681 gccgcccacc agcgcgacga ttgcaggcgc ggtcgccata gcaaatccac caataccgta
     4741 ggtttcggtg atggcgctgt cgccgatatc cagccccgaa tcttccggct tataaccggc
     4801 aaacatcggg ccgatcacct gctgcgccgg gccggtaaac cattgccccg gcaggccgct
     4861 gacccgcagg ccgaactcga cgccgttacg cgccatggtg gtgaccacgg tgctgtattc
     4921 gatgccgtgc gccgcatcca tcgccgcttt acacatcgcc atccacgtcg ggccggagaa
     4981 gtagtcgctg ctggcgacaa actcgaacac ttcgcgctgt tgctcgacgg aatagcctgc
     5041 ctgaatgatc cccggcgtca gtgcctgaat cagcagcgtc gtcccggcgt tattgcggtt
     5101 atggcactca tcgcccatat gcagcgcctg cgccagcatt aagcgcagat cgatctcacc
     5161 gataattttc atcgcgtcgc gcagcattgg cccctgcaca tcgcgcatcc agttgaggcg
     5221 gtcgatcacg ctctgatcgt tggcacccat acgcaaaatc ttcgccatct gctcgctcat
     5281 attggtgtaa gcgatgttgc cgtaggtttt gtttttcacg atgtgcataa acatcgaggc
     5341 cgaggtaacg cccgccatcg atcccacaca gtcgtgttcg tggcacggcg agaaggtgat
     5401 ctccccggag gccgccagtt cagccgcctc gtcgagatct ttcgccagtc cttcgaacac
     5461 cagcgctccg gtgaccgcgc ctttcatcgc gccgcacatt ttttcccagg tgatcggcgg
     5521 cccggcgtga agaatggttt tcggcgtcat gcccggcacc acgttaatcg cctgatcaaa
     5581 accaatcagc accggatgcg actggataat gcgctccagc gcctgctggt tagcggcggc
     5641 gattttgtct gccagcggcg aatcggcaat gttatccagc gcctgcacca cctgcatatt
     5701 gccctgcccc ggcggcgtcc agtcgagttg agtcacttct acatgctgct ttttcaggtc
     5761 atcgctaaac atggcgatgc cgacgttaat aacgttcaat ggttggctaa acagtgactg
     5821 gctcatcagg cttcctcccc tttgcagata aattcacgcg ccagcaatcc ggtattggtg
     5881 ctgctgctcg ccagaatcac tccggcatca agcagcatct ggctttgttg ttctaacgat
     5941 ggcgtatcaa gatcggtacc cagcacatag gcgagaatga tcaactcgcg tccttcggca
     6001 gcggcgatcg ctttcgcttc tttgatcgcc tcgatggtgg agccgaccgg atcttcatgc
     6061 gatccaaatc cgagcacaaa atccatcacg atcaccgcca cttctggatc gcgcgcctct
     6121 tcgatcaagc gactgatgcg gttggtgggg tcaatcattg gatgcggctt gccgttcgtg
     6181 aagtcgtcat cgccaaagtc gaggaaggtg tgtttgatgc tgcggttgat atcttgcagg
     6241 cggaattccg gatcgggctg aatgttgctg tagacatcgc catgtttttc catcaccgcg
     6301 aacatggttt cgtcgcacag cgtgccgccg cagaacaggc cacgaatgta tttctgctgc
     6361 ggttgcagac gcgcacgcac atcagcaatc aacggctggt taagcgtatg caggtcgaga
     6421 ttttcctgtt tcacgccgga gagcatcacc gcttttagcg ccgcctcttt gctgccgcgg
     6481 gcaaactgta gcccctgctc atccaccggc gtttcaacgc gaccgaggaa gcagaccacc
     6541 accggcttgc ggcaggcacg tgcgcgttcc agcactttgc gggccaccgc aggcgcggga
     6601 ggttttgaaa tcagtgcgat aatttcagtt tgcggatcgt tttccagcat cccgatggcg
     6661 tcgagcatca tcaggccgcc gattttctcg ctcaggtcgc gcccgccggt gccaatcagt
     6721 tgtgaaacac cgccaccaaa ttcatgaatg cggacgctca gctcctggct gccggtgccg
     6781 gatgcgccaa caataccgat attgccgcga cgcacggcgt taccaaaaca gagcgccgcg
     6841 ccgttaataa tcgccgtgcc gcagtccggc cccatcatca gcagcccttt ttcgtgggcc
     6901 aactgcttga gcgccagttc atcttcaact gagacgttat cggaaaacag catcacgttg
     6961 agatcgtttt gcagcgcctg acgcgcttcg cgagcggcaa acagaccgtt gaccgaaatc
     7021 accgccaggt tactttccgg gatatgcttt ttggcgctgg caatagtggc gtaacgcgcc
     7081 tcgtgcgagc cgctttgcgc tttggtgttg aacagttctt caatctccac cagcaactgc
     7141 tcgttgtccg cacccgattt accattgatg acaatcatca ggtcgccgtt tttcgcctgt
     7201 tccagcgtcg gcgtcagcag tcctaaattt ttcagcacac ctttgttcat ttcggtcgcc
     7261 atcgccacaa atgcctgctc gacgccgtcg agtttatttg cacgcgtgga gatagacatc
     7321 agcgagacag aatcaaaata ggtattcggt ttaatgacta ttttaactga cataattccc
     7381 tccgtaggtt tggctcagcg cacaaccatc ggccatgcca tacagcatgt cgcagccgga
     7441 actggagcca atatgtttaa ttttttctat tgcctgagtt gaattcccag ggacgccata
     7501 aataatgttg tgaataaagc gatgaatatt ttcccggcag cgttgttgta atgcggcttc
     7561 cagcgttatg gcacttaata acgtggtgtt atttctgccg cgtagcagac cgcgataaaa
     7621 ttcctctttg tatttttctg ccggatgccc gggaataaat aaaataagcg ccagacctgt
     7681 taaataatca tcggctgatg gcgtaaggcc aatccctaac ccaatcatac tggcgaccgc
     7741 tgcggcgata ttttgcttat cgtttactgt ctgaataact gcccgtcgtt taatatgtaa
     7801 ttctttattt aacgcctgat aaaacggatt atcgccgcga taattaaaca gggtttcatt
     7861 ttcccccagg tgctgatgaa taatgtcgtg ccactgtagc caggcgataa ggtgaaaatt
     7921 cacagcggtc atttgcaagg ttggggacag ccaacgccga caacgactca tctctatatg
     7981 aagatgttga ccaaccgtaa taccttgatc gtcaaactga accttatcac catgccggaa
     8041 cagatcatca aagtgagtga gtgccaaccg acaactgttt ggcgcattgt cgtactcttc
     8101 acaaagcagc gtcagcaact gctggcgagc cgggagtgcg atatttaccg ctctggcaaa
     8161 gacctgttca acctgtcctt gcccccgctg cgccagaaaa tccgcatccg cagttagcgc
     8221 ccacacacac cgtttttctg gtgtgttgat agccagcctc ccggtttatg cacctgcggc
     8281 aatcagtaac tgcgcaatct cttcaaagcc ccgttcccgc gccagttcca gtggcgtttt
     8341 gccatattta tcggtcagat gcgggctggc accgtgttcc agcaataact gcacaatcgc
     8401 ctgctgttta ataccaccat cattaagcac aatcgcttcc agtagcggcg tccagcctac
     8461 atggttggtc tggttaacgt taatctccgt atgcgccaaa agctctttta caatacttaa
     8521 atggcctttt tcacaggcag gcgtcaggcc gacaccgcca aaacgggtta cgcaattaag
     8581 atcgggttta gccggtaaaa taattcgtag tagcgttaaa tcatcgttca gacagctaat
     8641 taaaaaagga tttaaacagg tatgatcttg cttattaata tccgctccgg catcaattaa
     8701 tgcctgaacg caagcatatt gctgatataa acttgccagc gtaattgccg ttttcccctg
     8761 acgatcgcag gtattaatat cgacacccag cgcaaggcag gtttttactt tatcgatatc
     8821 accctgttgt gcagccaata aatagtcggc aggtagattt tttatactca ttcacattcc
     8881 cctggttttt gtggctgttt tcgatgaatt gagaatagca acgataatct gataaaagaa
     8941 cccgcttaaa tttaattcgc cgtatctcgc ctttttgttg aataatattc aacaatcttt
     9001 tcgcagtgtg cggtagtgca atatttgttt ctttgacccg ataccctttc actcaatcag
     9061 gttttatttc tctatagtct gcacatgtaa gagtagcttt cgtccatgtc taaccagagg
     9121 ggaacaagat gaacggactc acggcaacgg gtgtcaccgt cggtatttgt gcagggcttt
     9181 ggcagttagt gtcttcacat gtcgggctct ctcaaggatg ggaattatta ggaaccattg
     9241 gcttcgtcgc cttttgcagt ttttatgccg caggcggcgg taaatcgggg tttatcagaa
     9301 gtctggcggt taattattcc gggatggtgt gggcgttttt cgccgcgctg actgctggtt
     9361 ggctggcctc tgtaagtgga ctgtccgcgt tctgggcgag cgtaattact accgtaccct
     9421 tctcggcggt agtggtctgg caagggcgtt tctggttact gtcctttatt cccggtggct
     9481 ttctcggcat gacgctgttt tttgccagcg ggatgaactg gacggtgacg ttactgggtt
     9541 ttctggcggg taactgcgtg gggataattt cagaatatgg tggacaaaaa ttaagcgaag
     9601 cgacgactaa acgcgacggg tattaaaagt tattcaaaaa tgattatcta tctttaaccg
     9661 atacctctac agtcatagct ggagaaattc atagtagata atggcgagaa tggtttaatg
     9721 aactcaattt ttaccgaaga gaatctgttg gcatttacta ccgctgcgcg ttttggcagc
     9781 ttcagtaaag ccgcagaaga gttgggttta accacttccg ccattagcta caccatcaag
     9841 cgtatggaga cggggctgga tgtggtgctg ttcactcgca gtacccgcag cattgagtta
     9901 acggagtccg ggcgttattt tttccgcaaa gctaccgacc tgctgaatga tttttatgcc
     9961 atcaaacgca gtatcgatac tatttcgcag ggcatagaag cgcgtgtgcg catttgtatt
    10021 aatcagctgc tttatacgcc caaacacacc gcacgcttgt tacaggtgct gaaaaagcag
    10081 tttcctacct gccagattac cgtcaccacc gaagtgtata acggcgtctg ggatgcgatt
    10141 attaataatc aggccaatat cgccatcggc gcacccgata cactactgga cggcggcggt
    10201 attgattaca ctgaaattgg cgcgattcgc tgggcttttg ctatcgcccc ggatcacccg
    10261 ttagcctttg tgccggaacc catcgccgaa agccaactgc gtctctaccc taatattatg
    10321 gtggaggaca ccgcgcatac gattaataaa aaagtgggct ggttgctgca cgggcaggag
    10381 tcaattctgg tgccagattt caacaccaaa tgtcagtgcc agattttagg tgaaggaatt
    10441 ggttttttgc cggattacat ggtccgtgag gcgatgacgc aatccctgct ggttacccga
    10501 caaatccata atccgcgcca ggattcgcgc atgttactgg cgactcagca ttctgctacg
    10561 ggtcaggtga cgcagtggat taaaaaacaa tttgcgccaa atggcatatt aacggggatc
    10621 tatcaggatt tactgcatcg ggagaattaa tcgctgccaa cctcccgatg cgctgtgaat
    10681 agcggtttac atcaaccacc tgctttcatt acccattcag agataaattt attacccggt
    10741 actggcggag agtacaaata gccctgcaaa aagtgaacgc ctttaccgat cattaaatcc
    10801 gcctgctcct gggtttctac cccttccgcc acgatactca gaccaggctt acgcgctagt
    10861 tcgacaatat tgtccacaat atgaccggag atttcgtcga cactcgccat ttgcacaaat
    10921 gacttatcga tcttaataaa atcgaccggg aacgcctgca agtaacgata ggtcgcataa
    10981 cccgtaccaa agtcatccag cgcaaaggta atgttgtgct gatgaaggct gtcaaatatc
    11041 gctctggctt ctggcgttac cggaataggg ttacgttccg ttagctcgag aaccagcttg
    11101 attttatcgt tacctaattt cttaaccagg ttcagacact ctttttcaaa tcccgctgcc
    11161 aaaaaacaac ccgccgagac gttgatgcca atatggaaat tgtccggcag caaatgtttt
    11221 accggcatca gaatatccgc agtctgtttc atcagttggc gggtcattat gacaataaga
    11281 ccggatgact ccgccagagg aataaactga tccggtggga taattcccgt ttgtggatgt
    11341 tcccagcgga caagtacctc acagcccgtc agtaccccag tctgcgcgca gaaaaccggt
    11401 tggatccacg gtttgaattc gtggttttcc aacgccagac tgatggcttc aggcgtgacg
    11461 atatggtgac aacggttatc agggatataa aaattatttt tatttcccgt gccactgatg
    11521 acttgcggat gatattgaaa aaagatatcc cgccagaagg taatatcact ctggatgcct
    11581 agtttttcat acagctgctt cttttgatgt gaaattgtct ttgcactgcg atttctgtac
    11641 tgtgagattt ctgtaaccga cattccctcc attagcagct tgagtaaaag ccgttctttt
    11701 tcggacacga taccaggcaa cgtcaaattc accgttgccc cgaactcttc caaaaaaaca
    11761 ctcaaatcat gcgaagccat tgaacacacc ttgattaaat cttgtgcgcc agctaaatct
    11821 gcgagtcttc actctgactg ataaagctgg ctgcaagaaa atgacacaga gagccgaatt
    11881 atctcccgtt cggctctctc tccttttact cattattcag atcagaattg ccacttaatg
    11941 ccaaccattg cagaggtgtc attatatccc ctatcggcaa cctgaacgcc cacattcccc
    12001 cagacattca ggttggcatt tagctgtcct tccaccccgg tttttatctc agcaatattt
    12061 cgggctccat cctgagtgac ggacacgcca tccatactgg tgctgaaatc cttactgtta
    12121 tgcagccagt tcacttctac aaacggctgg aactcgcggg atttaccgtc atccatttta
    12181 tggtggctct tcagccaggt ttttacgcca agtcgggttt gaacattgcc atcaccgttg
    12241 ctatgaacga gggttccgtt gctttcgcgg tgcttatcgg ctttgactcc catccaggta
    12301 acctgtgctt gcggctgaac ataccattca ttacgcgttc cctggctgcc attaaattca
    12361 gctaatttgt gtttgtatcc agcttccagt gaagcggtaa atccttttga tttataggat
    12421 tcactttgta agtcatcccc tttcactgtg ttatcaaacc agctgtactg cgcccaactg
    12481 tcgagatacg cgccattacg cgactcgtca tcggcatacc aggtggcata gaggccggtg
    12541 ctatatccgt tcacactcgc ttttgcacga taaccggttc gcgaggaaat ggttttgctg
    12601 tcgctgttgc catatcccgc catgacccca acatgccagc tgtcgctgcc gttttggctc
    12661 cactgcgcga catcgcctcc cagttgcaga acatagcgat tgctttgggt tttcagctgg
    12721 ccgctgccat cacgccattt attatgacca ccttcatggc gcatccacat agtggtttgt
    12781 ttctgctcac ccgtcaccat gtcggtatag tacgtattac ccagacgctc atgcagacgc
    12841 gtggtgaaca ttgtattcgc tgctgcaagg ttcgcaatgt agctacccgc ctccggtcgc
    12901 aggtcattat ccacattcag atccggaccc ggcgtaggtg tcgggttagg gttcggctct
    12961 ggattcgggt ttggctccgg attcggcatc gggtctggct ccggctgcag ttcaggagaa
    13021 tcactgccgc tggtcagata ccagttacca ctatttgccc cttgtccacg cgcgagagtg
    13081 tagtcatacg cccccgcaac gatacgacca gcctgaacaa attcgccctc agacttaccg
    13141 tctacatgga taagttcgat accgttaagg gttttcgcac ctgtaccgcc agcgttattc
    13201 accgtaacgg cagtcgtgcc gctagtatcg ccctcgacaa ccaatttatc ggttactgag
    13261 tcatcgccat tcagtaccgt attcattact agttgaccgt tattaccgtg gtagttcccc
    13321 ttcaccacta acacattgcc agcttgctgt cctgacattc cggtcatcag cgtaccgttg
    13381 ttggtcagat tgccgcccag ggtgtaattc acagacgcag ccgctctttt ttcagcgcta
    13441 ttattcaacg ccagggtacc gttgttctca acgtttgcgc tacccaatcc ctgcgcatga
    13501 cttaccagga cattagccga attagcaatg ttcacattcg ctgtcaggtt gctgttatcg
    13561 cccgcgatgt ccagcgtgcc gccctgaata ttcagctctc cgctccccgt taacgaaccg
    13621 gttgaagtgc ccccgttggt aaccgtcaga ctgccgccat ttaagctcag cagtgaatcg
    13681 gcgctgccat tgagcgtgcc cacggtctgg ctgtgaccat tcatatccag ttcagtctct
    13741 gccgccagac gcagttcatg ggtattaccc aacacgttat cgttattcaa caaaagcgtc
    13801 ccactgcgca gatcggtaac accggtatag tcgttgtctt tgttagaaag cgatacggtc
    13861 tgacccttct ggctgctgaa tgccaggtca ccactgccgg taatctttgc gctgagatcg
    13921 gcggcattct cgcttttacc gttagagctc agcaccagag catcgctgtc ggtagcgtgt
    13981 aaatcaagct gggtcaggcc gtaaccgata tacagaccgt cattgtttac cccactcgtc
    14041 agacgatagt cgtaagtgcc gacggccgct ttttgcccat tctgctgaat atcacgttgg
    14101 gcactgctgg agaggatttg cccattttta tcctgcaatt gcagattgcc cgcatcgccc
    14161 agaacttccg caccttgcgc atcaaccagc ttaatggcgc tgttaccatc atcgacttca
    14221 gtgagcgata acgcagagtc aatatcacga gatactgagc ggacaacgtc actgtcagaa
    14281 acctgaatca caccctcgcc gcgcaaatcc agcgttttac taacgttgac cgtcccttct
    14341 gtcatctgtg cacctgctgt cagggcacca aaatcaacgg ttccgccgga gaatcccaaa
    14401 gtgctaatgg agtgagcgcc cttacccact gttaccaggc ttccctgacc cgcagtgatc
    14461 ccgcctctct gcagaaactc agcattcttt tccaggttaa atgtcgtgtt ctggaacgcc
    14521 agtgaaccct ggaaccagtc tgtataagca aactcatcga aggagaattc gtgattcccc
    14581 atgtttattt caacaatacc tctaccttga agatcgttat caaataccca atctttattg
    14641 gcattgattt gcagtttgcc atcaacatcc aggtccgcac ggccgatgtt cttgtcttca
    14701 gaaataaccg cgacagcacc agaagcgaca tcaatatatc cgctgaatgc atagttattg
    14761 ccggtcagcg ccactgtccc actgtcgata cttattgtac cgttgccgga aatcttattg
    14821 gcaaaggtca tgctatcgtc agccttcagg tgtaatttcc cgcggttgtt gatttctgcg
    14881 gtacctatat tttgggtgat ccctaaagcg ccattttgct caattttgac tttaccgcta
    14941 aaggcggagt tatccccggt taagtaagtc gtaccattga gcgcattgat atagcccagg
    15001 ccctcgctgt cactgacaat atccgcggaa atagtcgcgt ttttatccgt gtggtttaag
    15061 gtgattttct tctcggatgt ctttgcccac acaaactcaa ttttttcggc atcaataatc
    15121 cctgcagcct tcgctgcgct tccttcctgt gcacctaacg ctaactcaga gttggtgctt
    15181 aaactaatag tcggcgcaga gattttacca ccttccgaaa cagcaagtgt tgtatcggag
    15241 ttttgatcat aataacccag gctaattttc tcactggcat taacttcacc gccgttatta
    15301 actgacaggt actgattacc accgtaactg ccgtaaacgc ccataaagaa ggttttagaa
    15361 tttacctggc tgccttcacc actgacattt ccctgcaaat aggtcagacg gctggtgccc
    15421 ttaacatgga ttttgccgcc gttggagaca ttaaaatagc cgcgatcgtg tctcatgcct
    15481 gagtttgact catacaaatc atcagcatat aactcgctgc cagcattgga gacattcagg
    15541 aaccccg
//
LOCUS       DAZPBM010000093        15511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPBM010000093 DAZPBM010000000
VERSION     DAZPBM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.093"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHUZQ_002641"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHUZQ_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1026295.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHUZQ_002642"
     CDS             567..650
                     /locus_tag="ACHUZQ_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026296.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHUZQ_002643"
     CDS             750..1571
                     /locus_tag="ACHUZQ_002643"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1026297.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUZQ_002644"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUZQ_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1026298.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUZQ_002645"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUZQ_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1026299.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUZQ_002646"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUZQ_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1026300.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUZQ_002647"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUZQ_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1026301.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPAAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUZQ_002648"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUZQ_002648"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1026302.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUZQ_002649"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUZQ_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1026303.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHUZQ_002650"
     CDS             8347..9729
                     /locus_tag="ACHUZQ_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1026304.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHUZQ_002651"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHUZQ_002651"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1026305.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHUZQ_002652"
     CDS             complement(10485..10820)
                     /locus_tag="ACHUZQ_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1026306.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUZQ_002653"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUZQ_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1026307.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUZQ_002654"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUZQ_002654"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1026308.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHUZQ_002655"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHUZQ_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1026309.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUZQ_002656"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUZQ_002656"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1026310.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEQGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3811 a         4021 c         3793 g         3886 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcggcaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc ctgctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZPBM010000094        15442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPBM010000094 DAZPBM010000000
VERSION     DAZPBM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.094"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUZQ_002657"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHUZQ_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1026311.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHUZQ_002658"
     CDS             1598..4654
                     /locus_tag="ACHUZQ_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1026312.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHUZQ_002659"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHUZQ_002659"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1026313.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHUZQ_002660"
     CDS             5161..5349
                     /locus_tag="ACHUZQ_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1026314.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUZQ_002661"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHUZQ_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1026315.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUZQ_002662"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHUZQ_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1026316.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUZQ_002663"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHUZQ_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1026317.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUZQ_002664"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHUZQ_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1026318.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUZQ_002665"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHUZQ_002665"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1026319.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUZQ_002666"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHUZQ_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1026320.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUZQ_002667"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHUZQ_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1026321.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUZQ_002668"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHUZQ_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1026322.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHUZQ_002669"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHUZQ_002669"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1026323.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(14591..14785)
                     /locus_tag="ACHUZQ_002670"
     CDS             complement(14591..14785)
                     /locus_tag="ACHUZQ_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026324.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="ACHUZQ_002671"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="ACHUZQ_002671"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1026325.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3900 a         3738 c         4088 g         3716 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gacagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat ac
//
LOCUS       DAZPBM010000095        15406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPBM010000095 DAZPBM010000000
VERSION     DAZPBM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.095"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..453
                     /locus_tag="ACHUZQ_002672"
     CDS             <1..453
                     /locus_tag="ACHUZQ_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1026326.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="ACHUZQ_002673"
                     /pseudo
     CDS             669..758
                     /locus_tag="ACHUZQ_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHUZQ_002674"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHUZQ_002674"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1026327.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHUZQ_002675"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHUZQ_002675"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1026328.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHUZQ_002676"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHUZQ_002676"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1026329.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHUZQ_002677"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHUZQ_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1026330.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="ACHUZQ_002678"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="ACHUZQ_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1026331.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="ACHUZQ_002679"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="ACHUZQ_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1026332.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="ACHUZQ_002680"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="ACHUZQ_002680"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1026333.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="ACHUZQ_002681"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="ACHUZQ_002681"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1026334.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="ACHUZQ_002682"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="ACHUZQ_002682"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1026335.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="ACHUZQ_002683"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="ACHUZQ_002683"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1026336.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHUZQ_002684"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHUZQ_002684"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1026337.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHUZQ_002685"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHUZQ_002685"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1026338.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="ACHUZQ_002686"
     CDS             13055..13435
                     /locus_tag="ACHUZQ_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1026339.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="ACHUZQ_002687"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="ACHUZQ_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1026340.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHUZQ_002688"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHUZQ_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1026341.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /locus_tag="ACHUZQ_002689"
     CDS             complement(15244..>15406)
                     /locus_tag="ACHUZQ_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1026342.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4042 c         3851 g         3597 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt tagaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaata taatggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAZPBM010000096        15371 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPBM010000096 DAZPBM010000000
VERSION     DAZPBM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.096"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            92..2626
                     /gene="mrcB"
                     /locus_tag="ACHUZQ_002690"
     CDS             92..2626
                     /gene="mrcB"
                     /locus_tag="ACHUZQ_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1026343.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2846..5035
                     /gene="fhuA"
                     /locus_tag="ACHUZQ_002691"
     CDS             2846..5035
                     /gene="fhuA"
                     /locus_tag="ACHUZQ_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1026344.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            5086..5883
                     /gene="fhuC"
                     /locus_tag="ACHUZQ_002692"
     CDS             5086..5883
                     /gene="fhuC"
                     /locus_tag="ACHUZQ_002692"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1026345.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5883..6773
                     /gene="fhuD"
                     /locus_tag="ACHUZQ_002693"
     CDS             5883..6773
                     /gene="fhuD"
                     /locus_tag="ACHUZQ_002693"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1026346.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6770..8752
                     /gene="fhuB"
                     /locus_tag="ACHUZQ_002694"
     CDS             6770..8752
                     /gene="fhuB"
                     /locus_tag="ACHUZQ_002694"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1026347.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8910..10190)
                     /gene="hemL"
                     /locus_tag="ACHUZQ_002695"
     CDS             complement(8910..10190)
                     /gene="hemL"
                     /locus_tag="ACHUZQ_002695"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1026348.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10356..10403
                     /gene="yadX"
                     /locus_tag="ACHUZQ_002696"
     CDS             10356..10403
                     /gene="yadX"
                     /locus_tag="ACHUZQ_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1026349.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10415..11836
                     /gene="clcA"
                     /locus_tag="ACHUZQ_002697"
     CDS             10415..11836
                     /gene="clcA"
                     /locus_tag="ACHUZQ_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1026350.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11860..11925
                     /gene="yadW"
                     /locus_tag="ACHUZQ_002698"
     CDS             11860..11925
                     /gene="yadW"
                     /locus_tag="ACHUZQ_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1026351.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11918..12262
                     /gene="erpA"
                     /locus_tag="ACHUZQ_002699"
     CDS             11918..12262
                     /gene="erpA"
                     /locus_tag="ACHUZQ_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1026352.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12309..12932)
                     /locus_tag="ACHUZQ_002700"
     CDS             complement(12309..12932)
                     /locus_tag="ACHUZQ_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1026353.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12970..13770)
                     /gene="btuF"
                     /locus_tag="ACHUZQ_002701"
     CDS             complement(12970..13770)
                     /gene="btuF"
                     /locus_tag="ACHUZQ_002701"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1026354.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13763..14461)
                     /gene="mtnN"
                     /locus_tag="ACHUZQ_002702"
     CDS             complement(13763..14461)
                     /gene="mtnN"
                     /locus_tag="ACHUZQ_002702"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1026355.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14545..>15371
                     /gene="dgt"
                     /locus_tag="ACHUZQ_002703"
     CDS             14545..>15371
                     /gene="dgt"
                     /locus_tag="ACHUZQ_002703"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1026356.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         3474 a         3864 c         4291 g         3742 t
ORIGIN      
        1 cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa cagaagaaca gaaaatcggg
       61 cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg agccaattgg
      121 acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga
      181 tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg
      241 taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat ggctgctgtt
      301 aaaactggct atcgtttttg ccgtgctgat cgccatttac ggcgtttatc tcgatcaaaa
      361 aattcgtagc cgtattgatg gcaaggtctg gcaactgccc gcggcagttt atggccgaat
      421 ggtcaacctt gaaccagata tgaccatcag caagaacgaa atggtgaagc tgctggaggc
      481 aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc gaatttaccg tgcaggccaa
      541 cagcattgag atgatccgcc gtccgtttga tttcccggac agcaaagaag ggcaggtgcg
      601 tgcgcgtctg acctttgatg gcgatcatct ggcgacgatc gtcaatatgg agaacaaccg
      661 tcagttcggt ttcttccgtc ttgatccgcg cctgatcacc atgatctctt cgccaaacgg
      721 tgagcagcgt ctgtttgtgc cgcgcagtgg tttcccggac ttgctggtgg atactttgct
      781 ggcgacagaa gaccgtcatt tctacgagca tgatgggatc agtctctact ccatcggacg
      841 tgcggtgctg gcaaacctga ccgccggacg cacggtgcag ggagcgagta cgctgacgca
      901 acagctggtg aaaaacctgt tcctctccag cgagcgttct tactggcgta aagcgaacga
      961 agcttacatg gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc ttgagctgta
     1021 tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct tcccgcttgc
     1081 gagcttgtat tactttggtc gcccggtaga agagctcagc ctcgaccagc aggcgctgtt
     1141 agtcggtatg gtgaaagggg cgtccatcta caacccgtgg cgtaacccga aactggcgct
     1201 ggagcgacgt aatctggtgc tgcgtttgct acaacaacag cagattatcg atggtgaact
     1261 gtatgaaatg ctcagcgcgc gtcctctggg cgtacagccg cgcggtgggg taatctctcc
     1321 acagccagcc tttatgcaac tggtgcgtca ggagctgcag gcgaaattag gcgataaggt
     1381 aaaagatctc tccggcgtga agatcttcac tacctttgac tcggtggccc aggacgcggc
     1441 agaaaaagcc gccgtggaag gcattccggc actgaagaaa cagcgtaagt tgagcgatct
     1501 tgaaactgca attgtggtcg tcgaccgctt tagtggtgaa gttcgtgcga tggtgggagg
     1561 ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt cgattggttc
     1621 ccttgcaaaa ccagcgactt atctgacggc tttaagccag ccgaaaatct atcgcctgaa
     1681 cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg aatggccagg tctggtcacc
     1741 gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg atgctggtgg atgcgttgac
     1801 ccgttcgatg aacgtgccga cggtaaatct ggggatggcg ctggggctgc ctgcagttac
     1861 ggagacctgg atcaaactgg gcgtgccgaa agatcagctg catccggttc cggcgatgct
     1921 gctgggggcg ttgaacttaa cgccaatcga agtggcgcag gcattccaga ccatcgccag
     1981 cggcggtaac cgtgcaccgc tttctgcact gcgttcggta atcgcggaag atggcaaggt
     2041 gctgtatcag agcttcccgc aggcagaacg cgctgttccg gcgcaggcgg cgtatctgac
     2101 actatggacc atgcagcagg tggtacaacg tggtacgggt cgtcaacttg gggcgaaata
     2161 cccgaacctg catctggcag ggaaaacagg gactaccaac aataacgtag atacctggtt
     2221 tgcgggcatt gacggcagca cggtgaccat cacctgggta ggccgtgata acaaccagcc
     2281 gaccaaactg tatggtgcca gcggggcaat gtctatttat cagcgttatc tggctaacca
     2341 gacgccaacg ccgctgaatc ttgttccgcc agaagatatt gcagatatgg gcgtggacta
     2401 cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg ccggtctgga ccagcgatcc
     2461 gcaatcgctg tgccagcaga gcgagatgca gcagcagccg tcaggcaatc cgtttgatca
     2521 gtcttctcag ccgcagcaac agccgcaaca gcaacctgct cagcaagagc agaaagacag
     2581 cgacggtgta gccggttgga tcaaggatat gtttggtagt aattaacatc taagcgtgaa
     2641 ataccggatg gcgaattgcc atccggtaaa ataacatccc acctaaagta ttaacccttt
     2701 cttttcatct ggttgtttat taacccttca ggaacgctca gattgcgtac cgcttgcgaa
     2761 cccgccagcg tttcgaatat tatcttatct ttataataat cattctcgtt tacgttatca
     2821 ttcactttac atcagagata taccaatggc gcgttccaaa actgctcagc caaaacactc
     2881 actgcgtaaa atcgcagttg tagtagccac agcggttagc ggcatgtctg tttatgcaca
     2941 ggcagcggtt gaaccgaaag aagacactat caccgttacc gctgcacctg cgccgcaaga
     3001 aagcgcatgg gggcctgctg caactattgc ggcgaagcac tctgctactg cgactaaaac
     3061 ggatacacca attgaaaaaa cgccgcagtc tatttcggtt gtgacaaatg aagagatgca
     3121 gatgcgtcaa tttcagtctg taaaagaagc attaggttat acacctggcg ttacggttag
     3181 cagtcgtggt gcttctaata catatgattt tgttatcatt cgtggtttct catctgttgg
     3241 tctgaatcaa aataattacc ttgatggact aaaacttcag ggcaactttt ataacgatgc
     3301 tgtgattgat ccttacatgc ttgagagggt tgaactgatg cgtgggccga cgtctgttct
     3361 ctatggaaaa agtaatcctg gtgggattat ttctatggtg agtaagcgtc cgaccactga
     3421 acccctgaaa gaaattcagt ttaaaatggg gacggataat ctgtttcaga ccggattcga
     3481 tttcagtgat gcactggatg ataacggtga gttctcttac cgtttgacgg gcctcgctcg
     3541 ctcaacaaac gaacagcaga aaaactctga atctcagcgt tataccattg ctccatcatt
     3601 ctcatggcgt ccagacgaca aaaccaattt tactttcctg tcctatttcc agaatgaacc
     3661 agaaacgggt tattacggtt ggttgccgaa agaggggacc gttgagccat tgcctaatgg
     3721 taagcgtcta ccgactgact tcaatgaagg tgcatcgaat aatacatact cccgtaacca
     3781 gaaaatggtg ggatatagtt ttgaacatgg ttttaatgac accttcaccg tgcgtcagaa
     3841 tctgcgtttc agtgaaatga aaacctcaca gaaaagtgtt tatggcacag ggattgctgg
     3901 tgatggtcat accctaaacc gcgggacagt ggtggataat gagcgtctac aaaactttag
     3961 cgttgatacc caacttgaaa gtaagtttgc tacaggtgac gtcgagcata ctttgctgac
     4021 aggggtagac ttcatgcgga tgcgtaatga tatcaatgcc agctttggat ccgcaccatc
     4081 catcgatctt tataacaaat atcaccctga atactttgca tttggtagcg cagagccata
     4141 ccagatgaat gaaagcaaac aaacaggtat ttatgttcag gatcaggcgg aatggaataa
     4201 atgggtattc actctggggg gacgctacga ttggtctaag caagcgacca ctgttcgtga
     4261 aaactcttat acgccgactg aaggttatat tgagcgcaat gatcatcagt tcacctggcg
     4321 cggtggtgta aattacttat tcgataatgg tatttcacct tactttagct atagccagtc
     4381 ctttgaaccg agtgctttcg atctgtggag caacccgcgc atttcctata agccatcgaa
     4441 aggtgaacag tatgaagctg gcgtaaaata tgttccgaat gatatgccgg tcgttgttac
     4501 gggcgcagtc tatcaattga cgaaaacaaa taacctgaca gcagacccaa caaacccatt
     4561 agcgcaagtc ccagcaggtg agattcgcgc tcgtggtgtg gaacttgaag caaaagcggc
     4621 gttaaatgcc aatattaact tgacggcttc ttatacctac accgatgcgg aatacaccaa
     4681 agacaccaat ctcaaaggta aaactccaga acaagtaccg gagcatatgg catctctctg
     4741 gggggattat accttcaatg aagggccgct ttctggttta acattgggaa caggtggtcg
     4801 ttttattggt tccagctatg gtgatccggc aaacactttt aaagtgggta gcgcagctgt
     4861 aatggatgct gttgtaaaat atgatctggc acgctttggt atggcgggat ccagccttgc
     4921 tgtgaacgtc aacaatttgc tcgatcgtga gtatgttgcc agttgcttcc agacttatgg
     4981 ctgcttctgg ggcgcagaac gtcaggtcgt tgcaaccgca accttccgtt tctaatttct
     5041 cttttggggc acggatttcc gtgcccattt cacaagttgg ctgttatgca ggaatacacg
     5101 aatcattccg ataccacttt tgcactgcgt aatatctcct ttcgtgtgcc cgggcgcacg
     5161 cttttgcatc cgctgtcgtt aacctttcct gccgggaaag tgaccggtct gattggtcac
     5221 aacggttctg gtaaatccac tctgctcaaa atgcttggtc gtcatcagcc tccgtcagaa
     5281 ggggagattc ttcttgatgc ccagccgctg gaaagctgga gcagcaaagc gtttgcccgc
     5341 aaagtggctt atttgccgca gcagcttcct ccggcagaag ggatgaccgt gcgtgaactg
     5401 gtggcgattg gtcgttaccc gtggcatggc gcgctggggc gctttggtgc ggcagatcgc
     5461 gaaaaggtcg aggaagctat ctcgctggtt ggcttaaaac cgctggcgca tcggctggtc
     5521 gatagtctct ctggcggcga acgtcagcgg gcgtggatcg ccatgctggt ggcgcaggat
     5581 agccgttgtc tgttgctcga cgaaccgact tcggcgctgg atatcgccca ccaggttgat
     5641 gtgctggcgc tggtgcaccg tttaagtcag gaacgtggcc tgacggtcat tgccgtgttg
     5701 cacgatatca atatggcggc acgctactgt gattatctgg tcgccctgcg cagtggtgaa
     5761 atgattgctc agggaacgcc tgcggaaatt atgcgcggcg aaaccctcga aatgatttat
     5821 ggcatcccga tgggtatttt gccgcatccg gcgggtgctg cacctgtgag ttttgtttat
     5881 tgatgagcgg cttacctctt atttcgcgcc gtcgactgtt aacggcgatg gcgctttctc
     5941 cgttgttatg gcagatgaat accgcccacg cggcggctat tgaccccaat cgtattgtgg
     6001 cgctggagtg gttgccggtg gaattactgc tggcgctcgg catcgtgcct tacggcgtgg
     6061 cggataccat caactatcgc ctgtgggtca gcgaaccacc attgccggac tcagtgattg
     6121 acgtcggttt gcgtacagaa cctaaccttg aactgctgac cgaaatgaaa ccgtcgttta
     6181 tggtctggtc ggcaggatat ggcccttcat cagaaatgct ggcacgtatt gcgccgggtc
     6241 gcggatttaa cttcagtgac ggcaaacagc cgttggcgat ggcgcgtaaa tcgctgacgg
     6301 aaatggcaga tttacttaac ctgcaaagcg cagcggaaac gcatttagcg caatacgaag
     6361 actttatccg cagcatgaaa ccccgctttg tgaagcgtgg cgcacgcccg ttactgctga
     6421 cgacgcttat cgatccgcgc catatgctgg tcttcggtcc aaacagcttg ttccaggaaa
     6481 ttctcgatga gtacggcatc ccaaatgcct ggcaagggga aaccaacttc tggggcagta
     6541 ccgccgtcag tatcgatcgt ctggcagcgt ataaagacgt tgatgtgctc tgttttgatc
     6601 acgacaacag caaagacatg gatgcgctaa tggcaacccc gctgtggcag gccatgccgt
     6661 ttgtccgcgc cggacgcttt cagcgcgtac ctgcagtctg gttttatggt gcgacactct
     6721 cggcaatgca ctttgtgcgc gttctggata acgccatcgg aggtaaagcg tgagtaaacg
     6781 aattgcgctt tttccggcgt tattgctggc gctgttagtg attgtcgcta cggcgctcac
     6841 ctggatgaac ttctcgcagg cgctgccgcg tagccagtgg gcgcaggctg cctggtcgcc
     6901 agacatcgat gtcatcgagc agatgatttt tcactacagc ttgttgccgc gtctggcgat
     6961 ttcgctgctg gtgggcgcgg gcctggggct ggtgggcgtg ctgtttcagc aagtgctgcg
     7021 taacccgctg gcggagccga cgacgcttgg cgttgctaca ggcgcgcaac tgggaattac
     7081 cgtcaccaca ctttgggcga tcccaggtgc tatggcgagc cagtttgccg cactggtggg
     7141 cgcttgtgtc gttggcttaa tcgtctttgg cgtcgcgtgg gggaaacggc tttcgccggt
     7201 tacgcttatt ctcgcggggc tggtggtgag cctttattgc ggggcaatta atcagctact
     7261 ggttatcttc catcacgacc agctgcaaag catgttcctg tggagcaccg gaacgctgac
     7321 gcaaaccgac tggggcggcg ttgagcgttt atggccgcag ttgctgggcg gcgtgatgtt
     7381 gacgttattg ctacttcgcc cgttaaccct gatggggctt gatgatggcg tggcgcgcaa
     7441 tctcgggctg gccttgtcgc tcgcgcgtct ggcggcgctg tcgctggcga tagtcatcag
     7501 tgcgctgctg gtgaatgcgg tggggattat cggctttatc ggtttgttcg cgccgctgct
     7561 ggcgaaaatg ctgggggcgc ggcgtttgtt gccacggttg atgctggcgt cgctgattgg
     7621 ggcgctgatc ctctggcttt ccgatcaaat catcctctgg ctgactcgcg tgtggatgga
     7681 agtgtccacc ggttcggtca ctgcgttgat cggtgcgccg ctgctactgt ggctgttgcc
     7741 gcgtttacgc agcattagcg cgccggatat gaaggtcaac gatcgtgtcg cggctgaacg
     7801 ccaacatgtg ctggcgtttg ccctcgcggg cggcgtgctg ctgttgatgg ctgtggtggt
     7861 ggcgctgtcg tttggtcgtg atgcgcacgg ctggacgtgg gcgagcgggg cgttgctcga
     7921 ggatttgatg ccctggcgct ggccgcgaat tatggcggcg ctgtttgcgg gcgtcatgct
     7981 ggcggtggcg ggctgtatta ttcagcggct gaccggaaac ccgatggcaa gcccggaagt
     8041 gctggggatt agctctggcg cggcgtttgg cgtggtgtta atgctgtttc tggtgccggg
     8101 taatgccttt ggctggctgt tacccgcggg cagtatcggg gcggcggtga cgctgttgat
     8161 cattatgatc gccgccggcc gcggtggatt ttccccacac cgtatgttac tggcggggat
     8221 ggcgttaagt accgcattca ccatgctttt gatgatgttg caggcgagcg gtgacccgcg
     8281 aatggcgcaa gtgctgacct ggatttccgg ttcgacctac aacgcgaccg atgcgcaggt
     8341 ctggcgtacc ggaattgtga tggtgatttt gctggcgatt accccgctgt accgccgctg
     8401 gctgaccatt ttaccgctgg gtggtgatac cgcacgagcc gtaggaatgg cgctgacgcc
     8461 gacgcgaatt gcgctgctgc tgttagcggc ttgcctgacg gcgaccgcga cgatgactat
     8521 tggaccgttg agttttgttg gtttaatggc accgcatatt gcgcggatga tgggctttcg
     8581 acggacgatg ccacacatcg taatttcggc gctggtgggt ggtttactgc tggtattcgc
     8641 tgactggtgt gggcggatgg tgctgtttcc attccagatc ccggcggggc tgctgtcgac
     8701 ctttatcggc gcgccatatt ttatctattt gctgagaaag cagagccgtt aatttttcca
     8761 tataacggcc ctgtacgcct ggacggataa agcgtttacg cgacaacgga tgcccgatgc
     8821 gacgctggcg cgtcttatcg ggcctacaaa cggccccgaa tcgtaggtcg gataaggcgt
     8881 tcacgccgca tccgacaaac catgctggat cacaacttcg caaacacccg acgtgcagca
     8941 tcgatggtgt tattgatatc ttccatgctg tgcgccacgg acataaagcc cgcttcaaac
     9001 gctgacggtg ccaggtaaac accttcgtcc agcatcatat ggaagaaacg cttaaagcgt
     9061 tccacgtcac aggccatcac atcctgatag cacgtcacgg actcggcgtc ggtaaagaaa
     9121 ataccgaaca tgccgccaac gtggttaacg accagcggaa ttccggcttc ttctgccgct
     9181 tccagcagac cttctgccag acgtgttgtc agctcatcca acgtttcgtg aacgcccggt
     9241 tgcgcgactt cattcagaca ggcgaaaccg gctgccatcg caattgggtt accggaaagc
     9301 gtacccgcct gatagaccgg acccgtcggg gccagcgcat ccattacatc acgacgacca
     9361 ccgaatgcgc ctaccggcat tccaccgccg atgattttgc ccaggcaggt gagatccggt
     9421 tccacaccgt aataatcctg tgcgccagcc agtgccacgc ggaagccggt cattacttca
     9481 tcgatgatca gcaatgcgcc aaattcgtcg cacagcgcac gcagacctgg caggaagtct
     9541 ggcagcggtg gaacacagtt catattgcct gccaccggct cgatgataat acaggcaatc
     9601 tcttgcgggt attgctcaaa cgcggcgcgt acagaagcca gatcgttata agtacaggtt
     9661 aaggtatgtt tggcgaaatc tgccggaacg cccggcgagt ttggctggcc taacgtgagt
     9721 gcgccagaac cggctttcac cagcaggcag tcagcgtgac cgtggtaaca cccttcaaat
     9781 ttaatgattt tgtcgcgacc ggtaaaacca cgggccagac ggatggcgct catcgtcgcc
     9841 tcggtgccgg agttcaccat gcgcaccata tccatggtcg ggaccaattc ggtcaccagt
     9901 tgcgccattt tcacttccat ttcggttggt gcaccaaagc ttaaaccacg ctcggcggct
     9961 tcaatcacgg cattgcggat tgccggatgg ttatggccca gcaccatcgg cccccaggaa
    10021 ccgacataat cgatataggc tttgccatca acatcgtaca gataagcgcc gtccgctttt
    10081 tcgataaaca gtggagtgcc gcccacgcca gtaaaggcgc gaacagggga gttcacaccg
    10141 ccagggatca gctcgcgcgc tgcgctgtaa agattttcag acttactcat ggagggttcc
    10201 tgattcgtag aaaaagtgaa tggctgctat tctatgttat tcataacaag ttaaatactc
    10261 gtcaaacatc aggctgcttg tactggtgca atcctgattt cttagagtat aaaagttttg
    10321 tgcatttgaa acattacgct ttgcaaagga ttttcatgga acgtgcgagt aaaatgccgt
    10381 catcttattt gtatgaccaa taagtgatca ttggatgaaa actgatactc cctctttaga
    10441 aacaccgcag gccgcgcgcc tgcgacgcag acaactgatt cgccaacttc ttgagcgcga
    10501 taaaaccccg ttagccattt tgtttatggc ggcagtcgtc ggcacgcttg ttgggctggc
    10561 agcggttgct tttgacaaag gtgtcgcctg gttgcagaac caacgtatgg gggcgctggt
    10621 acatactgct gataattatc cgcttctgtt aaccgtcgct tttctctgtt cggcggtgct
    10681 ggcgatgttt ggctactttt tggtgcgcaa atacgcgccg gaagcaggtg gttcggggat
    10741 cccggaaatt gaaggggcgc tggaagatca acgtcccgtt cgctggtggc gtgtattgcc
    10801 ggtgaagttc tttggcgggc tggggacact cggcggaggc atggtgttgg ggcgcgaagg
    10861 gccaaccgtg cagatcggcg gtaacattgg ccgtatggtg cttgatattt tccgcctgaa
    10921 aggtgacgaa gctcgccata cgctgctggc aaccggtgct gctgcggggc tggctgcggc
    10981 ctttaacgcg ccgctggcgg gtattttgtt tattatcgaa gagatgcgtc cgcagtttcg
    11041 ctatacgtta atttcgataa aagcggtatt tattggcgtc attatgtcga ccattatgta
    11101 ccggattttt aatcatgaag ttgcgttgat tgacgtcggt aaactttctg atgcgccgct
    11161 taatacgctg tggctttatc tgatcctcgg tattattttt ggcattttcg gccctatttt
    11221 taataaatgg gtgctgggga tgcaggattt gctgcaccgt gtgcacggcg gcaatattac
    11281 caaatgggta ctgatgggcg gtgcgattgg cggtctgtgt ggattgctgg ggtttgtggc
    11341 accagcaacg tcgggcggcg gttttaacct gattcctatc gctaccgcgg ggaatttcag
    11401 catgggaatg ctggtgttta tcttcgtcgc tcgggtcatt accaccttac tctgcttctc
    11461 ttccggcgcg ccgggcggta tttttgcccc gatgctggcg ctgggtactg tgctgggaac
    11521 cgctttcgga atggttgccg ttgagctgtt tccgcaatat caccttgagg cggggacgtt
    11581 tgctattgcc ggaatggggg cattactggc ggcatctatt cgcgcgccgt taacggggat
    11641 cattctggtt ctggagatga ccgataacta ccagctcatt ttgccaatga ttattaccgg
    11701 tcttggcgca acactattag cgcaatttac cggcgggaaa ccgctatact cggcgattct
    11761 tgcgcgcacg ctggcaaaac aggaagctga gcaactggcg cgaagcaagg ccgcatcagc
    11821 cagcgagaat acttgaacga aataccaggg tattagataa tggcgattat tattgggtta
    11881 gaatttgccc aattgccgat gtcgtttgga gcaaaatatg agtgatgacg tagcactgcc
    11941 gctggagttt accgacgcag cagccaacaa agttaaaagc ctgatcgctg acgaagataa
    12001 cccgaatctg aaattacgcg tgtatatcac aggtggcggt tgcagcggct tccagtatgg
    12061 tttcaccttt gatgatcagg tgaacgaagg cgatatgacc atcgaaaaac agggcgttgg
    12121 cttggtggtt gatccgatga gcctgcaata tctggtcggc ggttccgttg actataccga
    12181 aggactggaa ggttctcgtt tcatcgtgac caacccgaac gcgaaaagca cctgcggttg
    12241 cggttcttcc tttagcatct aatctgttgt tcatgtgccg gataaactat ccggcgcatc
    12301 ttctctcttc aacgcccatt ctcatccagc gcaaacgtcg gtagcttaag atgccaacga
    12361 atagccgcca gccgaatcaa tagcgtcacg accatgccca tcatactggc tgtttccagt
    12421 ggtacggaaa atgtgtaata agccgtagcg tggacaatac cgccgataat acaggcagtt
    12481 gcgtagattt ctgtacgtaa aatcatgggg atctcgcggg ccagtacatc acgaatgatc
    12541 ccgccgccaa cgccagtaat gacgaccata caaaccgcga ttaacggacc gacttccgca
    12601 ttaaaggctt tattcacgcc aatgccgaca aacaccgcca gaccaacggc gtccagcacc
    12661 ggcaacatcc attttggtaa gcgtcttggc tggcgcacca gcacgatggt cagcatgctg
    12721 gttaccattg caacgaccag atcggtggga tctttcaccc aaaataccgg gccgtgatcc
    12781 agcgccatgt cgcgaattgt cccgccgcct acagcggtaa ccacgcccag taccagaaca
    12841 ccaaaagggt ccatacgcaa ttttccggct aacaaaacgc cggagatggc aaataccgct
    12901 gtgccgacta tatccagcca atagacgagc attgttcaat ccccactgag cacctaaaaa
    12961 ggtcaggcgc taatctacct gtgaaagcgc attacagagc tgttgtgcag cgaggataat
    13021 acgtgggctt gcacgttcaa accagtcact cgtgagagga ataacgggaa ttttgagctg
    13081 ttcaccccag tattgtttga ttttaggaat ttggtccggt ccgcctgtaa tgacaatcgc
    13141 ctgtggcgag cgtgctaaca cctgttcgcg gctaacttgc ggccagggaa cccggctgtc
    13201 tttaaagatg ttttctccgc cacaaacttc gagtacctgg ttttgaatcg actcttttcc
    13261 actggtaaat ggcggattaa tgccgaattg cagaaaaaca cgttttttag gtttatcagc
    13321 atattgcgct ttcaattgcg cgtactgatc cagcagggat tgcgcggctt gttcggcctt
    13381 atccggttgc ggactccagg gggccaattg acgtaacgca ttggcaattt gttcaatgct
    13441 tgtcgcatcg acccacatca cttttattcc cagcgaagcc agctggtcaa cctgccgctc
    13501 ggcattacct ccacgccagg caatcaccag atcgggtttc agcgcgacaa tgcgttccag
    13561 attcatcccc tgccaggtgg aaacctgctc aatcttttgc gcttgtggag gatagtcgga
    13621 atagctgctg accccaaccg gcgtgatccc ggcggcaaag gcaagttcag tgttggcggg
    13681 agaaagcgtg atgacgcgcg gcgcggcgtt gagccacagt ggcgcaagaa aagacagggc
    13741 gaccagcgcc ctgaacagtg acttagccat gtgccagttt ctgcaccagt gactcaacca
    13801 tcaggctgga ctgtttagcg gcaacagcca ggaactcatc gaagctaaga tgagactgtt
    13861 gatcggccac gtcggagatg gcgcgtacga caacaaacgg gacgttgaaa ttgtggcaga
    13921 catgggcgat tgccgtcgct tccatctcta cagcaatggc ctgtgggaag ttgtggcgga
    13981 ttttcgccag accaacagaa ccgttgatga aagcgtcgcc gctaacaatc aggccacgta
    14041 cagcgttaag attcagttcg gcaatgcagg cctcagcggc agcgatcagt ttatcgtcag
    14101 ctttaaagcc tgccggacag cctggtaact gaccgtattc ataaccaaat gccgtgacat
    14161 ccgcgtcgtg ataacgcgct tcgtccgaga caacgatatc gcccactttc agcgttggtg
    14221 ccaggccacc ggcagaaccg gtgttaataa tcacatctgg cttgcagtgt tccaacagca
    14281 gagtggcacc cagcgccgca gcgactttac cgatgcccga tttcagaagc gcaacctcgg
    14341 ttccattcag ttggccggta tagatttcgc aaccgccgag actgatagtt tgacggtttt
    14401 cgattttgtc acgcagcagc gtaacttctt cttccattgc accaatgatg ccgattttca
    14461 tagatttact cgcgataagc ccgatttgaa agcatagttt accatgcgct tacggggaag
    14521 cgtatttctc acgcgggaga ggacatggca cagattgatt tccgaaaaaa aataaactgg
    14581 catcgtcgtt accgttcacc gcagggcgtt aaaaccgaac atgagatcct gcggatcttc
    14641 gagagcgatc gcgggcgtat catcaactct ccggcaattc gtcgtctgca acaaaagacc
    14701 caggtttttc cactggagcg caatgccgcc gtgcgcacgc gtcttaccca ctcgatggaa
    14761 gtccagcagg tgggacgcta catcgccaaa gaaattttaa gccgtctgaa agagcttaaa
    14821 ttgctggaag catacggcct ggatgaactg accggcccct ttgaaagcat tgttgagatg
    14881 tcatgcctga tgcacgatat cggcaatccg ccgtttggtc attttggcga agcagcgata
    14941 aatgactggt ttcgccaacg tttgcacccg gaagatgccg aaagccagcc tctgactgac
    15001 gatcgctgca gcgtggcggc actacgttta cgggacgggg aagaacagct taacgagctg
    15061 cggcgcaaga ttcgtcagga cttatgtcat tttgagggga atgcacaagg cattcgtctg
    15121 gtgcatacat tgatgcggat gaatctcact tgggcacagg ttggcggtat tttaaaatat
    15181 acccgtccgg cgtggtggcg tggcgaaacg cctgagacac atcactattt aatgaaaaag
    15241 ccgggttatt atctttctga agaagcctat attgcccggt tgcgtaaaga acttaatttg
    15301 gcgctttaca gtcgttttcc attaacgtgg attatggaag ctgccgacga catctcctat
    15361 tgtgtggcag a
//
LOCUS       DAZPBM010000097        15347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPBM010000097 DAZPBM010000000
VERSION     DAZPBM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.097"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHUZQ_002704"
     CDS             <1..1015
                     /locus_tag="ACHUZQ_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1026357.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUZQ_002705"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUZQ_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR1026358.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHUZQ_002706"
     CDS             1797..1904
                     /locus_tag="ACHUZQ_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1026359.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUZQ_002707"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUZQ_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1026360.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUZQ_002708"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUZQ_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1026361.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUZQ_002709"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUZQ_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1026362.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUZQ_002710"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUZQ_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1026363.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUZQ_002711"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUZQ_002711"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1026364.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHUZQ_002712"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHUZQ_002712"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1026365.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIGSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUZQ_002713"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUZQ_002713"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1026366.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHUZQ_002714"
     CDS             complement(11226..11813)
                     /locus_tag="ACHUZQ_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1026367.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHUZQ_002715"
     CDS             11983..12561
                     /locus_tag="ACHUZQ_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1026368.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHUZQ_002716"
     CDS             complement(12691..13452)
                     /locus_tag="ACHUZQ_002716"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1026369.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUZQ_002717"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUZQ_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR1026370.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHUZQ_002718"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHUZQ_002718"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1026371.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3745 a         4179 c         3840 g         3583 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag ataatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcggca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcg
//
LOCUS       DAZPBM010000098        15231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPBM010000098 DAZPBM010000000
VERSION     DAZPBM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.098"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHUZQ_002719"
     CDS             267..1925
                     /locus_tag="ACHUZQ_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1026372.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHUZQ_002720"
     CDS             complement(1922..2878)
                     /locus_tag="ACHUZQ_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1026373.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHUZQ_002721"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHUZQ_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1026374.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHUZQ_002722"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHUZQ_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1026375.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUZQ_002723"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUZQ_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1026376.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUZQ_002724"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUZQ_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1026377.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUZQ_002725"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUZQ_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1026378.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUZQ_002726"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUZQ_002726"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1026379.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHUZQ_002727"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHUZQ_002727"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            10061..10999
                     /gene="ghrA"
                     /locus_tag="ACHUZQ_002728"
     CDS             10061..10999
                     /gene="ghrA"
                     /locus_tag="ACHUZQ_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR1026380.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            11054..11791
                     /gene="ycdX"
                     /locus_tag="ACHUZQ_002729"
     CDS             11054..11791
                     /gene="ycdX"
                     /locus_tag="ACHUZQ_002729"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1026381.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            11815..12369
                     /gene="ycdY"
                     /locus_tag="ACHUZQ_002730"
     CDS             11815..12369
                     /gene="ycdY"
                     /locus_tag="ACHUZQ_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1026382.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            12471..12962
                     /locus_tag="ACHUZQ_002731"
     CDS             12471..12962
                     /locus_tag="ACHUZQ_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1026383.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(13027..13860)
                     /gene="csgG"
                     /locus_tag="ACHUZQ_002732"
     CDS             complement(13027..13860)
                     /gene="csgG"
                     /locus_tag="ACHUZQ_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR1026384.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(13887..14303)
                     /gene="csgF"
                     /locus_tag="ACHUZQ_002733"
     CDS             complement(13887..14303)
                     /gene="csgF"
                     /locus_tag="ACHUZQ_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1026385.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(14328..14717)
                     /gene="csgE"
                     /locus_tag="ACHUZQ_002734"
     CDS             complement(14328..14717)
                     /gene="csgE"
                     /locus_tag="ACHUZQ_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1026386.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(14722..>15231)
                     /gene="csgD"
                     /locus_tag="ACHUZQ_002735"
     CDS             complement(14722..>15231)
                     /gene="csgD"
                     /locus_tag="ACHUZQ_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1026387.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         3747 a         3551 c         4073 g         3860 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgtcgttcc
     9841 tgtcttgctg gaacgggcgc taatttaggg aaatcatgac ctgaggtcaa caaacttttt
     9901 gaaaaaatcg cgcgtttatt caaacttcaa tcaatgtgtg gttttaataa gcgaaatctg
     9961 ctttttttgc caccgaccac ggatttgtta tgctggtggc ctttgtagat cataacgata
    10021 agtgcgaata aatttcgcac aacgcttttc gggagtcagt atggatatca tcttttatca
    10081 cccaacgttc gatacccaat ggtggattga ggcactgcgc aaagctattc ctcaggcaag
    10141 agtcagagca tggaaaagcg gagataatga ctctgctgat tatgctttag tctggcatcc
    10201 tcctgttgaa atgctggcag ggcgcgatct taaagcggtg ttcgcactcg gggccggtgt
    10261 tgattctatt ttgagcaagc tacaggcaca ccctgaaatg ctgaaccctt ctgttccact
    10321 ttttcgcctg gaagataccg gtatgggcga gcaaatgcag gaatatgctg tcagtcaggt
    10381 gctgcattgg tttcgacgtt ttgacgatta tcgcatccag caaaatagtt cgcattggca
    10441 accgctgcct gaatatcatc gggaagattt taccatcggc attttgggcg caggcgtact
    10501 gggcagtaaa gttgctcaga gtctgcaaac ctggcgcttt ccgctgcgtt gctggagtcg
    10561 aacccgtaaa tcgtggcctg gcgtgcaaag ctttgccgga cgggaagaac tgtctgcatt
    10621 tctgagccaa tgtcgggtat tgattaattt gttaccgaat acccctgaaa ccgtcggcat
    10681 tattaatcaa caattactcg aaaaattacc ggatggcgcg tatctcctca acctggcgcg
    10741 tggtgttcat gttgtggaag atgacctgct cgcggcgctg gatagcggca aagttaaagg
    10801 cgcaatgttg gatgttttta atcgtgaacc cttaccgcct gaaagtccgc tctggcaaca
    10861 tccacgcgtg acgataacac cacatgtcgc cgcgattacc cgtcccgctg aagctgtgga
    10921 gtacatttct cgcaccattg cccagctcga aaaaggggag agggtctgcg ggcaagtcga
    10981 ccgcgcacgc ggctactaat aaagcatcag gattcctgct atccttggcg ggaattgaat
    11041 acaggagaga gttatgtatc ccgtcgacct tcatatgcat accgttgcca gcacacatgc
    11101 atatagcaca ttgagtgatt acattgccca ggccaaacaa aagggcatta aactttttgc
    11161 gatcaccgat catggcccgg atatggaaga tgctccgcat cactggcact tcattaacat
    11221 gcgtatctgg ccgcgagtgg ttgatggggt agggatcctg cgcggcatcg aagctaacat
    11281 taaaaatgtt gatggtgaaa ttgattgcag cggtaaaatg tttgactcgc tggatctgat
    11341 tattgccggt tttcatgagc cggtttttgc accacatgac aaagcgacca atacacaagc
    11401 gatgatcgcc actatcgcca gcggcaatgt gcatataata agccatcccg gaaatcccaa
    11461 atatgaaata gatgtgaaag ccgttgctga agcagccgcg aaacatcagg tggcgctgga
    11521 aatcaataat tcttcatttt tacactcacg taagggcagt gaagacaact gtcgtgcggt
    11581 agccgcagcg gtacgtgatg ctggaggttg ggtggcatta ggctcggatt ctcacaccgc
    11641 gtttaccatg ggggaatttg aagagtgtct taaaatcctc gacgcggtag attttccgcc
    11701 agagcgcatt ttgaatgttt ctccgcgccg cttactgaac ttccttgaat ctcgcggtat
    11761 ggcaccgatt gcggaatttg cagaccttta attactagta atggaaatat attaatgaac
    11821 gagttttcta tcctctgtcg tgtgctgggt tcgctctatt accgccaacc gcaagatcct
    11881 ttactggtgc cgctgtttac cctgattcgt gaggggaaac tggctgcgaa ctggccactg
    11941 gagcaggatg agttactgac gcgtttacag aaaagctgtg atatggcgca agtctctgcc
    12001 gattacaatg cgttgtttat cggcgatgaa tgtgcagtgc cgccatatcg tagcgcatgg
    12061 gttgaggacg taacggaagc ggaagtgcgc gcttttcttt ccgaacgagg gatgccactg
    12121 gcggatacgc cagccgatca catcggcaca ttgctgcttg cggcttcctg gctggaagat
    12181 cagtcaacgg aagatgagag cgaagcactg gaaacactgt tcagtgagta tctgttaccc
    12241 tggtgtggtg cgttcctcgg caaagtggag gcccatgcaa ccacgccttt ctggcgcacc
    12301 atggcaccgc taacccgtga tgccattagt gcaatgtggg acgagctgga agaagattct
    12361 gaagagtaaa tgtgatcatc gtcactcata actgcaacag ataatttgtt gttgcataaa
    12421 atgtgtgctc gatctcattc atggccgcgt tttctgctat catgcgcggc atgaacatac
    12481 ttctctctat tgcaatcaca acaggcattc tctccggtat ctggggatgg gtggctgttt
    12541 ccctgggctt actaagctgg gcgggcttct tgggctgtac ggcctacttt gcctgcccgc
    12601 aaggtgggct gaaagggctg gcgatctccg ctgcaaccct gcttagtggc gtggtgtggg
    12661 cgatggtcat tatttacggt agtgcactgg caccacatct ggaaattctc ggttatgtca
    12721 taaccggtat tgtggcgttt ctgatgtgta ttcaggccaa acagctgctg ctttcatttg
    12781 ttccggggac gtttataggc gcatgcgcga cgtttgccgg gcagggtgac tggaaactgg
    12841 tgttaccttc gctggcgctt gggctggtat ttggttacgc aatgaaaaac agcggcctgt
    12901 ggctggcggc gcgtagtgca aagacggctc accgtgagca ggaaatcaaa aataaagcgt
    12961 gaggggcact cacgctttcg cttaaacagt aaaatgccgg attattaatt ccggcatttt
    13021 tatctgtcag gattctggtg gaaccgacat atggcggtat ttaaccagaa tgtcattctg
    13081 ccgttctgct ttgttctgca aatcccacag accacggtcg ataccatcat taatcaggaa
    13141 aatgacccct gtttcgatag ccgacatcag acacagcata acgggttcgt ttgaggtgta
    13201 acccacttcc ccttccagca agcgctggta gtcaataaag cggaaaaccc cggcctgaac
    13261 ttcataggaa agtatcgtct tactggtgtt caccgaggag aggatctcac cggtactcac
    13321 attgacgaca cgcaggttca cggcaatctg atcgagctgg tattgcgtgt ctgcaccgat
    13381 cccaaaatat cttgccccaa caccgcccga tttaacgttg ctttcataac cgataatcga
    13441 accttcaacc atgatatttg ccgccgttaa agattgcagc gggattcggt tattaatggc
    13501 aactgtgccg ttttcttgtg ccgcacgaat aatcttgcgc tcgttaagca ggttttgtaa
    13561 actctgacgc tccagcggta taaaccagcg agaatctttc aatgccgtga ccagcattgc
    13621 cgtggcgctt tgcggaacag cagtggagaa gttacttgcc gggtagggtt taaattgccc
    13681 ggtttcgtcc tgaatgttgt ataccgaaac aaagatctta cccgtcggcg ctggcagatg
    13741 ggtcaaatct ttgtagctct gagctcgagg cattaatgtc ggtctggcgg cttctttagg
    13801 cggggcggtt aagcatccgc tcagtaacat gacggcaacc aaaagaaata agcgctgcat
    13861 gattattttc cttatgaagc tggggcttaa aaatcggttg agttattttg taaacccgaa
    13921 acctggatgg tcgaggtttg tccggtttta cgatctgtca cgttcaactg caattgacca
    13981 tcgcggttag caatatcaac aatataatcg ttggtcacca tgcggcccgg tttaccggta
    14041 ttaatattcg acagtagccc acctaaaatt tgtgactgga tggcctgagt aaagttatct
    14101 aacgctgagg gtgtttcaat accaaagtca tcgttatagc tcggatcttt ataagagttt
    14161 tgggcctgag cgctatttaa taaaaaagcg ccattatttg ggttaccacc aaagtttgga
    14221 ttacggaact ggaaagtcat ggttccagcc caacttaatg gcgaaataag catgagtaga
    14281 actactgcat gtttgacacg cattgcagcc tccgaacaat tttttattta gaattcatca
    14341 tgcgccaaat cgcccgtact taataacgcc tgatttatct ggcgacgatt tagtgcttct
    14401 tcagtttgaa tcagtgcaaa gacgacagtt ttctcgaagt ctcttttcaa cggaaataaa
    14461 aaagtctgga aaataacgtc ctgattgacc gttatagtga tccagcttcc ccatcgtgca
    14521 ctgggccttt cattaatcgt taagttaccc gtatagtcac tttcccattt atcactaaag
    14581 gctcggtaaa aatcatggcc aatagatgaa acagtatggt cagttagcaa tcccgggact
    14641 tctacctcaa cggcgtgaag attccctgcg gcgaacagaa attctgccgc cacaatccag
    14701 cgtaaataac gtttcatggc tttatcgcct gaggttatcg tttgcccagg aaaccgcttg
    14761 tgtccggttt tttacggcta tcttcttgaa aagattataa agatgcgttt taaccgtatt
    14821 ttcgctgatg aacaacgaac gagcgatctc gttattagac gcgccgatac gcagtttatt
    14881 caggatctct ttttcccgat gagtaaggag ggctgattcc gtgctgttat aacgatagtt
    14941 acctgaatgc gtaatcaagt agctggcaag cttttgcgta aagtagcatt cgccgcgcag
    15001 gacgccttgc aacccattga caacacgttc ttgatcctcc atggcataaa aaacgccgtt
    15061 gatatgaggc cagttttcaa tgtcgcggta cgggtaatct tcaggcgtat ttagcaacaa
    15121 tattttgata ttgttgtttt tcctgctcaa agtatcctgc caataatgga taagcttttt
    15181 atccgcttcc atcatatcca gaagaataat agagcctgaa gatatatcgt c
//
LOCUS       DAZPBM010000099        15169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPBM010000099 DAZPBM010000000
VERSION     DAZPBM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.099"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUZQ_002736"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUZQ_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1026388.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUZQ_002737"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUZQ_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1026389.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUZQ_002738"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUZQ_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1026390.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUZQ_002739"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUZQ_002739"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1026391.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAECGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUZQ_002740"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUZQ_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1026392.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHUZQ_002741"
     CDS             complement(8214..9107)
                     /locus_tag="ACHUZQ_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1026393.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUZQ_002742"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUZQ_002742"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1026394.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUZQ_002743"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUZQ_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1026395.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUZQ_002744"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUZQ_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1026396.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUZQ_002745"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUZQ_002745"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1026397.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUZQ_002746"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUZQ_002746"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1026398.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15169)
                     /locus_tag="ACHUZQ_002747"
     CDS             complement(14275..>15169)
                     /locus_tag="ACHUZQ_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR1026399.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3560 a         4283 c         3739 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgca ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaat
//
LOCUS       DAZPBM010000100        15024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPBM010000100 DAZPBM010000000
VERSION     DAZPBM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.100"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            115..357
                     /gene="yjbE"
                     /locus_tag="ACHUZQ_002748"
     CDS             115..357
                     /gene="yjbE"
                     /locus_tag="ACHUZQ_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1026400.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            471..1109
                     /locus_tag="ACHUZQ_002749"
     CDS             471..1109
                     /locus_tag="ACHUZQ_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1026401.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1106..1843
                     /locus_tag="ACHUZQ_002750"
     CDS             1106..1843
                     /locus_tag="ACHUZQ_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1026402.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1843..3939
                     /locus_tag="ACHUZQ_002751"
     CDS             1843..3939
                     /locus_tag="ACHUZQ_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1026403.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3986..4264)
                     /locus_tag="ACHUZQ_002752"
     CDS             complement(3986..4264)
                     /locus_tag="ACHUZQ_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026404.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4265..4408
                     /locus_tag="ACHUZQ_002753"
     CDS             4265..4408
                     /locus_tag="ACHUZQ_002753"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026405.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4534..4944
                     /gene="psiE"
                     /locus_tag="ACHUZQ_002754"
     CDS             4534..4944
                     /gene="psiE"
                     /locus_tag="ACHUZQ_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1026406.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4988..6463)
                     /gene="xylE"
                     /locus_tag="ACHUZQ_002755"
     CDS             complement(4988..6463)
                     /gene="xylE"
                     /locus_tag="ACHUZQ_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1026407.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6835..7725)
                     /gene="malG"
                     /locus_tag="ACHUZQ_002756"
     CDS             complement(6835..7725)
                     /gene="malG"
                     /locus_tag="ACHUZQ_002756"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1026408.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7740..9284)
                     /gene="malF"
                     /locus_tag="ACHUZQ_002757"
     CDS             complement(7740..9284)
                     /gene="malF"
                     /locus_tag="ACHUZQ_002757"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR1026409.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9438..10628)
                     /gene="malE"
                     /locus_tag="ACHUZQ_002758"
     CDS             complement(9438..10628)
                     /gene="malE"
                     /locus_tag="ACHUZQ_002758"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1026410.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10993..12108
                     /gene="malK"
                     /locus_tag="ACHUZQ_002759"
     CDS             10993..12108
                     /gene="malK"
                     /locus_tag="ACHUZQ_002759"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1026411.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12180..13520
                     /gene="lamB"
                     /locus_tag="ACHUZQ_002760"
     CDS             12180..13520
                     /gene="lamB"
                     /locus_tag="ACHUZQ_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1026412.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13672..14592
                     /gene="malM"
                     /locus_tag="ACHUZQ_002761"
     CDS             13672..14592
                     /gene="malM"
                     /locus_tag="ACHUZQ_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1026413.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14821..15024
                     /locus_tag="ACHUZQ_002762"
                     /pseudo
     CDS             14821..15024
                     /locus_tag="ACHUZQ_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3753 a         3842 c         3933 g         3496 t
ORIGIN      
        1 aaacaccatc gctaattccc cgcgccgtaa ttcgcatgct ttagttgtgt atactcgatc
       61 ccgcccgaaa tgtttttggg taaatctcca ttcattcaat gaagggaaat tgttatgaaa
      121 aaagttctgt atggcatttt tgccatatct gcgcttgcgg cgacttctgt gtgggctgca
      181 cctgtacagg tgggcgaagc ggcaggggcg gcagcaacgt cggtttcggc ggggagttcc
      241 tccgcgacca gcgtcagcac cgtaagctcg gcggtgggtg tcgcgctcgc ggcaaccggt
      301 ggcggtgatg gttctaatac cgggaccaca acaaccacaa ccaccagtac ccagtaataa
      361 agtatgtatc cccaaaataa ttcgagtcat tgcatctgtg gcttgaaata tgaagggatt
      421 aaccataacc acactccggt gtggttattc tgcccctctg gagaagagtc gtgaagcgac
      481 ctgcactcat tcttatctgc ctgttattac aggcctgttc agccacgact aaagagctgg
      541 gcaattcact gtgggacagt ctgttcggca cgccaggcgt acagctgacg gacgatgata
      601 ttcaaaatat gccctacgcc agccagtaca tgcagcttaa tggcgggccg caattgtttg
      661 tggtgctggc cttcgctgaa gacggacaac aaaaatgggt cactcaggat caagccactc
      721 ttgtcacaca acatggtcgt ctggtgaaga ctttgcttgg cggcgacaac ctgattgaag
      781 tgaataacct tgccgccgac ccgctgatta aacccgcaca aattgttgat ggtgcaacat
      841 ggacgcgcac gatgggctgg accgagtacc agcaggtacg ctacgccacc gcacgctcag
      901 tcttcaaatg ggatggcacc gataccgtca aggtcggcag cgatgaaacc ctggttcgcg
      961 tgctggacga agaagtctcc accgaccagg cgcgctggca taaccgctac tggatcgaca
     1021 gcgaagggca aattcgccag tcggaacagt atctcggcgc ggattatttc ccggtgaaaa
     1081 ccactctcat caaggcggca aaacaatgat taaacaaact attgtcgcgt tgcttttgag
     1141 tgtgggagcg tcatcggtct tcgcggcagg aaccgtcaag gtgttcagca atggcagcag
     1201 tgaggccaaa acgctaacgg gcgcagagca tttaatcgat ctggtagggc aacctcgact
     1261 ggcaaacagc tggtggcctg gtgcggtgat tagcgaagag ctggcaacgg cggcagcatt
     1321 gcgtcagcag caggcgttgc tgacacggct ggcagaacag ggcgcagatt ccagcaccga
     1381 cgatgccgcc gccattaacg ccttacgcca gcaaatccag gcattggaag tgacaggcag
     1441 gcaaaaaatc aatcttgatc ccgatatcgt ccgcgttgcc gaacgcggta acccgccgtt
     1501 gcagggcaac tacacgctgt gggtcggacc accgccgtcc acggtcacgt tgttcgggct
     1561 tatcagccat cctggcaatc agccattcac tccaggtcgc gacgtggcga gctatctctc
     1621 tgaccagagc ctgctcagcg gtgcggatcg cagctacgcg tgggtcgttt acccggacgg
     1681 acgcacgcaa aaagcgccgg tggcttactg gaacaagcgt cacgtagagc cgatgcccgg
     1741 cagcattatt tatgttggcc tcgcagactc cgtctggagt gagacgcctg atgcccttaa
     1801 cgccgacatt cttcagactc tgacgcagcg gataccccaa taatgaaaaa aagacatctg
     1861 cttagcttac tggcgctggg cattagcaca gcttgctacg gcgaaacata tcctgcgccc
     1921 attggtccgt cgcagtcgga tttcggtggc gtaggattat tacaaactcc caccgcgcgt
     1981 atggcacggg aaggggagtt gagtctgaac tatcgcgata acgatcagta ccgttattac
     2041 tcagcttcag tgcaactctt cccgtggctg gaaacaacgc tgcgttacac cgacgtgcgc
     2101 acccggcagt acagcagcgt cgaagcgttc tctggcgatc aaacgtataa agataaagcc
     2161 ttcgatctca aactgcgttt gtgggaagag agttactggc tgccgcaagt ggcggttggt
     2221 gcgcgggata tcggcggtac ggggctgttt gatgcggaat atcttgttgc cagcaaagcc
     2281 tgggggccgt tcgattttac gctcggcctg ggctggggat atctgggcac cagcggtaat
     2341 gtgaaaaatc cgctctgttc agccagtgat aaatattgct atcgcgataa cagctacaaa
     2401 caagcgggat ctatcgacgg cagtcagatg ttccacggcc cggcctcact gtttggcggc
     2461 gtggaatacc agacgccctg gcaaccgctg cgcctgaaac tggagtatga aggtaataat
     2521 tatcagcagg attttgccgg gaagctggag caaaaaagta agtttaacgt cggggcgatt
     2581 tatcgcgtta ccgattgggc cgacgttaac cttagctatg aacgtggcaa cacctttatg
     2641 tttggcgtca cgctgcgcac caactttaac gatctgcgcc cgtcctacat cgataacgcc
     2701 cgcccgcaat atcaaccgca gccgcaggat gccattttgc agcactcggt ggtggcgaat
     2761 cagttaacgt tgctgaaata caatgccgga cttgccgatc cgcagatcca ggcgaaaggc
     2821 gatacgctgt atgttaccgg cgagcaggtg aaatatcgtg attcgcgcga agggatcatc
     2881 cgtgctaatc ggatcgtgat gaacgatctg ccggatggga tcaaaacgat ccgcattacg
     2941 gaaaatcgcc ttaacatgcc gcaggtgacg acggaaaccg atgtcgccag cctgaaaaat
     3001 catctcgccg gagagccgtt gggccacgaa acgacgctgg cgcaaaaacg cgtcgagcca
     3061 gtggttccgc agtccaccga gcagggctgg tatatcgaca aatcacgctt tgatttccat
     3121 atcgatccgg tgctgaacca gtcggtcggt ggcccggaaa acttttacat gtatcagctg
     3181 ggcgtgatgg gaacggcaga tttgtggctg acggaccatc tgctgaccat cggcagcctg
     3241 tttgcaaatc ttgccaacaa ctacgacaag tttaactaca ccaatccgcc acaggactcg
     3301 cacttaccgc gcgttcgtac ccatgtgcgc gagtatgtgc agaacgatgc ctatgtgaat
     3361 aacctgcaag ccaactactt ccagcatctg ggcaacggct tctacggtca ggtctatgga
     3421 ggttatctcg aaaccatgtt tggcggtgcg ggggctgaag tgttgtatcg tccgctggac
     3481 agcaactggg cgtttggtct ggatgccaac tacgttaaac agcgcgactg gcgtagtgca
     3541 aaagatatga tgaaattcac cgactacagc gtgaaaaccg gacatctgac cgcctactgg
     3601 acgccatctt tcgcccagga cgtgttagtg aaagccagcg tcgggcagta tctggcaggg
     3661 gataaaggcg gcacgctgga gatcgccaaa cgctttgata gcggcgtggt ggtgggtggc
     3721 tatgccacga tcacgaatgt ttcaaaagag gagtacggcg aaggggactt caccaaaggc
     3781 gtgtatgtct cggtaccgtt ggatctcttc tcgtctggcc cgacacgcag ccgtgcggcg
     3841 attggctgga cgccgctgac gcgtgacggt ggtcagcaac ttgggcgtaa gttccagttg
     3901 tatgatatga ccagcgaccg tagcgtcaat ttccgctaag tcatgggaaa ggtgccagtt
     3961 ttcgcaatca ctggcaccct cattcttaat ggcctgttga gccatcagga acgcagttca
     4021 cccccggttt gcgatctggt gtgtaaaggc gaaaacattc tgtcttcctg tccgtcgata
     4081 tcggttgcag gtctggttgc ctgagcgact gggccatcat tgggctggag acattgcttt
     4141 gcggtaggtc ggctttatca gcagtaccga gcggaccagc ataagcagga agaacagaga
     4201 ctgataacat caaagcagca aaataaggct tcatttttac cacctttatc aggttacgtt
     4261 tcatttgttc cagaggaaca ttgtcgattt ttcgcgcatt gctggtggct gggaatcacc
     4321 tgaatgggtg atttttgaat taccggcttg ggtgcggttt gtcttgccgg atgcgccgcc
     4381 aggcgcggct tatccggcct acgggtaggt atatccggct ttggtggagg cgcgctccaa
     4441 atccaggttg aacaaaacat acacaaaaaa tatagatctc cgtcacattt ttgcgttata
     4501 caggaagctc gccactgtga aggaggtact gctatgacgt cactctctcg tccgcgcgtg
     4561 gagtttatct ccaccatttt gcagaccgta ctcaatcttg gcctgctgtg cctcggcctg
     4621 attttggttg tcttcctcgg caaagaaacg gtgcatctgg cagatgtgct gttcgcgcca
     4681 gaacaaacca gcaaatatga gctggtagaa ggactggtgg tttactttct ctatttcgaa
     4741 tttatcgcgc tgattgtgaa gtactttcag tccggttttc acttcccgct acgttacttt
     4801 gtctatattg ggatcaccgc cattgtgcgc ttgatcatcg tcgatcataa atcgccactg
     4861 gatgtgttga tctactcggc tgcgatcctg ttactggtga tcactctgtg gctgtgtaat
     4921 tcgaaacggc tgaagcggga gtaaaaagaa agcacgccga actggcgcgg cgtgctggac
     4981 aggaagatta cagcgtagca gtttgttgtg ttttcttcgt ttccggttcc cagagggctt
     5041 ccagctcctc aagggtttta cctttggttt ccgggacaaa tttccacata aatagtgctg
     5101 ccagaacgcc catacacccg taaatccagt aggagaaacc gttgtggaaa tgggccacca
     5161 gccaggagtt tttgtccatc atcgggaagg tccaggagac gaagtagttc gccagccact
     5221 gggccgccac cgcgattgcc agcgctttac cacgaatagc attcgggaag atttccgaca
     5281 gcagtaccca gcataccgga ccccaggaca tggcaaaggc agcaacatag aacagcatcg
     5341 acagtagcgc cacaataccc ggtgcctgag tgtaaaacgc ggtaccgagg ctaaacatac
     5401 cgattgccat tccgagtgcg ccgataattt gcagtggctt acgaccaaat ttatccaccg
     5461 tcataattgc cagaacggtg aaggtgaggt tgataactcc gacaataatg gtctgcaaca
     5521 gcgcgatatc cgtgctggcc cccagcgttt tgaacacttc cggcgcgtag tacagcacca
     5581 cattgatgcc gacaaattgc tggaagatgg agagcataac gccgatgaca atcacgccca
     5641 cgccaaacat cagcagacga ccaccggttt tgcggccatg atccagggag tgtttaattt
     5701 cctgtactgc ctgagttgca agcgtgttgc ccataatttt gcgcaggata ctttccgcct
     5761 gttcttgctt gccgcgtgac atcagccagc gaggactttc tggcacggta tacagcagca
     5821 ttaagaacag cagtgcaggg atacattccg aggcaaacat ataacgccag ccgtcagtat
     5881 tcagccagct ggcatcaccg gaacgggcaa taaaatagtt tacgcagtaa actaaaagtt
     5941 gcccgaaaat aatcgcaaac tggttaaatg aaaccagttt tccgcgaata tgagctggag
     6001 ccagttccgc aatatacatt ggtgaaagca ttgaggctaa accaacgcca ataccgccaa
     6061 taatacgata aataacaaat tccgggacat aacctgccag ataaataggt actgtgttgt
     6121 ccgggtttat agaggtaaaa ccaagttctg gccaggcaga acctacacca gaaataaaaa
     6181 acaggacagc agcaatctta agtgaatcac gacggccgaa gcggttactg caataaccac
     6241 cgagggcacc gccgatgatg caaccaatca gagcgctggc tacgcaaaat cctaacaggg
     6301 agttggcagc ggattcactt aagttttgtg gagcaacaaa gacggtattg agtgactcaa
     6361 cagtaccgga aataacggcg gtgtcgtagc caaataataa accacctaat gtagcgacta
     6421 aggtaatcga aaatatataa ctggaattat actgggtatt cattcagacc tgccttagac
     6481 cattctgatc ttttatcagt gcattgatgt tggaattgtc aggcagttaa ggcaattatc
     6541 attttttaca atgcgtttac gtaatgtctt ttctgtgatc ttaattgtga taattatcaa
     6601 aaattgatga aaacaatact attgccgtga ctcagagcac gaaagagaat tatcgtaagt
     6661 gggaaaacaa ataacgtaaa aataataagc tctatgatgg aaatattaac cggcgaacga
     6721 ttcagattgc agacgaaaga aaaaaaggcg ctccgtggag cgccgaataa cagtcacaag
     6781 ttgggataac gtaagttgag ggtgcagcgg cataacattg gcagaacaac atctttaacc
     6841 tttcacacca cctgccgtca ggccgttgac cagccagcgt tgagccagca agaagacgat
     6901 ggtgatcggt aatgcagaca tcacggcagc ggcggcaaag tcaccccaca ggtagttttg
     6961 cgggttgagg tattgctgca tccccacggc cagggtgtag ctgtttacgt cacgcagtaa
     7021 cagcgacgcg accggaactt cagtaatggc agcgataaac gacaggatga ataccaccgc
     7081 cagaatcggt actgacagcg gcaacaggac aaggcggaag gcctgccacg gtgtcgcacc
     7141 atccagcgca gccgcttctt ccagcgaact atcgatggtt tcgaaatagc ctttgatggt
     7201 ccagacatgc agcgcaatcc cacccagata cgcgaaaatt acgccgccgt gagtattcag
     7261 gccaatgaat ggaatgtact cacccagacg atcaaacaac gcatacaacg cgaccagtga
     7321 aagtactgcc gggaacatct ggaaaatcag cattcctttc agcagcgtcg ctttgcctgg
     7381 aaagcgcata cgggcgaaag cgtaggcgca ggtggtagag agcgcgacaa tgccaatcgc
     7441 ggaaatcccg gcgaccttta ccgagttcca tagccacagc agtaccggga atggcggtgg
     7501 cgtaatgcga ccatcagcct gttccacgct aaaacctaaa gccagtttcc agtgatccca
     7561 ggagatttgc tccgggatca ggctgccgat cgcaaagttc ccctggcgca gtgagatagc
     7621 gacgaccatc agcagcggga acataatcgc cgcgataaaa agtagcagta gcaggtgagt
     7681 aataaacaaa cgtgcttttt gcgatttcgg ttggaccatt gccatttttg ttatctccct
     7741 taatcaaact tcattcgcgt ggctttcagg ttcactatcg ccagcgcacc caccagcagg
     7801 aagagcagcg tggcaattgc tgcagccaga ccgaagtcct gacccccgcc gccttcaaaa
     7861 gcgatacggt aggtgtagtt aacaagcagg tcggtataac cggctggcgt ggtcgtgcca
     7921 agacgatccg ggccgccgtt ggttaacagt tgaatcagca cgaagttgtt aaagttaaag
     7981 gcgaagctgg cgatcatcag cggcgtcagc ggtttaatca gcagcggcag cgtaatctta
     8041 aagaagttct ggaacggacc tgcgccatcc attgctgagg cttcatacaa atcgtccgga
     8101 atcgctttca gcaagcccat gcagaggatc atcatgtacg gataacccag ccaggtattg
     8161 acgataatta gcatcgtgcg ggcggtggtc ggatcgctga accaggcggg cttcacgcca
     8221 aacagcgcgc tcaacatcat gttgatttca ccgaagctct ggttaaacaa ccctttgaaa
     8281 atcaagattg aaatgaatga tggcaccgcg tagggcagaa tcagcagaac gcgatagacc
     8341 gctttgccgc gcaatgcttc ccactgcacc agacatgcca gaaccatgcc gaccgccacc
     8401 gttaaaaaga cagtgatcag cgagaacacc acggtccaga cgaaaatggc gaggaacggt
     8461 ttctgaatgc cttcgtcggt gaagacgcgg gtaaagtttt tccagccagt agtcacggtg
     8521 taaccggggc ttagcttttc atcaccccag ttgccgtcgg cggtaatgga ctggtaaaag
     8581 ccaatttggt tattcggacg atatttcacg ccgctctgat tattcgtcaa cgtgccgtca
     8641 ccgtcgagtg tgtagagcgg ctgcgtgcca gaaaactggc gcagggagct catcatcact
     8701 ttgttgccat ccggcagaat ggcggtaatg tcactcagcg cctgacgatt ctgggtaatc
     8761 acgcgtagat tcgcgcgttc gccttcgggc tgggcggtcg tttctttcag ttgcagtttt
     8821 tgttcgccgc caaatttaaa cgcgtcggag aggtaatttt tgccgatttc gccgtcactg
     8881 agcgccagtt gccactcatc gcccgccggg taaagaccaa agttataggt tttgcctgct
     8941 tgccaggagc gatctaacaa cacttcctgc gcacattcaa aagtcagctg gttagtgctg
     9001 ctgtagttgg tgaaggcaat ggcgatggtg cagaccagag ggaagaggac gaataatccc
     9061 attccagcca ttcccgggta aacatagcgc caggcgtagg ctttacgatt ggcgaaaata
     9121 tacagccccg ctgaactcaa tatcagcgtg gtaatggcga acaggtattc cccttgtgcg
     9181 tacattaaaa caacaaggta acccaccagc aggccgagca gacctagcac tgaccatttc
     9241 agcgcgtcgc tttgccacca atgtttcttt ttaatgacat ccatggggtt cttcctcatt
     9301 ccaggacgga taaggctttc acgccttatc cgacaacaac tgcctgatgc gacactgatg
     9361 cgtcttatca ggcctacatg cgtttcagtt ttgtaggccg gacaaggcgt tcacgccgca
     9421 tccggcattt cacagcatta cttggtgata cgagtctgcg cgtctttcag ggcttcatcg
     9481 acagtctgac gaccgctggc ggcgttgatc accgcagtac gcacggcata ccagaaagcg
     9541 gacatctgcg ggatgttcgg catgatttca cctttctggg cgttttccat ggtggcggca
     9601 atacgtggat ctttcgccaa ctcttcctcg taagacttca gcgccactgc acccagcggt
     9661 ttgtctttat tgaccgcttc cagaccttcg tcagtcagca gatagttttc gaggaactct
     9721 ttcgccagct ctttgttcgg actggcggcg ttaataccgg cgctcagcac gccaacgaac
     9781 ggtttagatg gttgaccctt gaaggtcggc agtaccgtta caccataatt cactttgctg
     9841 gtgtcgatgt tggaccatgc ccacgggccg ttgatggtca tcgctgtttc gcctttatta
     9901 aaggcagctt ctgcgatgga gtaatcggtg tctgcattca tgtgtttgtt tttaataagg
     9961 tcaaccagga aggtcagacc cgctttcgcg ccagcgttat ccacggccac gtctttaata
    10021 tcgtacttgc cgttttcata cttgaacgca taacccccgt cagcagcaat cagcggccag
    10081 gtgaagtacg gttcttgcag gttgaacatc agcgcgctct tacctttcgc tttcagttct
    10141 ttatccagcg ccgggatctc ttcccaggtt tttggtgggt tcggcagcag atctttgtta
    10201 taaatcagcg ataacgcttc aacagcgatc gggtaagcaa tcagcttgcc gttgtaacgt
    10261 acggcatccc aggtaaacgg atacagcttg tcctggaacg ctttgtccgg ggtgatttca
    10321 gccaacaggc cagattgagc gtagccacca aaacggtcgt gcgcccagaa gataatgtca
    10381 gggccatcgc cagttgccgc aacctgaggg aatttctctt ccagtttatc cggatgctca
    10441 acggtgactt taattccggt atctttctcg aatttcttac cgacttcagc gagaccgtta
    10501 tagcctttat cgccgttaat ccagattacc agtttacctt cttcgatttt ggcgagagcc
    10561 gaggcggaaa acatcatcgt cgttaatgcg gataatgcga ggatgcgtgc acctgttttt
    10621 attttcataa tctatggtcc ttgttggtga agtgctcgtg aaaacaccta aacggactct
    10681 agtttcttta tacggcaacc tctttccatc ctccttgccc ctacgcccca ccgttgcttt
    10741 gtgtgatctc tgttacagaa ttggcggtaa tgtggagatg cgcacataaa atcgccacga
    10801 tttttgcaag caacatcacg aaattcctta catgacctcg gtttagttca cagaagccgt
    10861 gttctcatcc tcccgcctcc tcccccataa aaaagccagg gggtggagga tttaagccat
    10921 ctcctgatga cgcatagtca gcccatcatg aatgttgctg tcgatgacag gttgttacaa
    10981 agggagaagg gcatggcgag cgtacagctg caaaatgtaa cgaaagcctg gggcgaggtc
    11041 gtggtatcga aagatatcaa tctcgatatc catgaaggtg aattcgtggt gtttgtcgga
    11101 ccgtctggct gcggtaaatc gactttactg cgcatgattg ccgggcttga gacgatcacc
    11161 agcggcgacc tgttcatcgg tgagaaacgg atgaatgaca ctccgccagc agaacgcggc
    11221 gttggtatgg tgtttcagtc ttacgcgctc tatccccacc tgtcagtagc agaaaacatg
    11281 tcatttggcc tgaaactggc tggcgcaaaa aaagaggtga ttaaccaacg cgtcaaccag
    11341 gtggcggaag tgctgcaact ggcgcatttg ctggatcgca aaccgaaagc gctctccggt
    11401 ggtcagcgtc agcgtgtggc gattggccgt acgctggtgg ccgagccaag cgtatttttg
    11461 ctcgatgaac cgctctccaa cctcgatgct gcactgcgtg tgcaaatgcg tatcgaaatc
    11521 tcccgtctgc ataaacgcct gggccgcaca atgatttacg tcacccacga tcaggtcgaa
    11581 gcgatgacgc tggccgacaa aatcgtggtg ctggacgccg gtcgcgtggc gcaggttggg
    11641 aaaccgctgg agctgtacca ctatccggca gaccgttttg tcgccggatt tatcggttcg
    11701 ccaaagatga acttcctgcc ggtaaaagtg accgccaccg caatcgatca agtgcaggtg
    11761 gagctgccga tgccaaatcg tcagcaagtc tggctgccag ttgaaagccg tgatgtccag
    11821 gttggagcca atatgtcgct gggtattcgc ccggaacatc tactgccgag tgatatcgct
    11881 gacgtcatcc ttgagggtga agttcaggtc gtcgagcaac tcggcaacga aacccaaatc
    11941 catatccaga tcccttccat tcgtcaaaac ctggtgtacc gccagaacga cgtggtgttg
    12001 gtagaagaag gtgccacatt cgctatcggc ctgccgccag agcgttgcca tctgttccgt
    12061 gaggatggca ctgcatgtcg tcgactgcat aaggagccgg gcgtttaagc accccacaaa
    12121 acacacaaag cctgtcacag gtgatgtgaa aaaagaaaag caatgactca ggagatagaa
    12181 tgatgattac tctgcgcaaa cttcctctgg cggttgccgt cgcagcgggc gtaatgtctg
    12241 ctcaggcaat ggctgttgat ttccacggct atgcacgttc cggtattggc tggacaggta
    12301 gcggcggtga acaacagtgt ttccagacta ccggtgctca aagtaaatac cgtcttggca
    12361 acgaatgtga aacttatgct gaattaaaat tgggtcagga agtgtggaaa gagggcgata
    12421 agagcttcta tttcgacact aacgtggcct attccgtcgc gcaacagaat gactgggaag
    12481 ctaccgatcc ggccttccgt gaagcaaacg tgcagggtaa aaacctgatc gaatggctgc
    12541 caggttccac catctgggca ggtaagcgct tctaccaacg tcatgacgtt catatgatcg
    12601 acttctacta ctgggatatt tctggtcctg gtgccggtct ggaaaacatc gatgttggct
    12661 tcggtaaact ctctctggca gcaacccgct cctctgaagc tggtggttct tcctcttttg
    12721 ccagcaacaa tatttatgac tataccaacg aaaccgcgaa cgacgttttc gatgtgcgtt
    12781 tagcgcagat ggaaatcaac ccgggcggca cattagaact gggtgtcgac tacggtcgtg
    12841 ccaacctgcg tgataactat cgtctggttg atggcgcatc gaaagacggc tggttattca
    12901 ctgctgaaca tactcagagt gtcctgaagg gctttaacaa gtttgttgtt cagtacgcta
    12961 ctgactcgat gacctcgcag ggtaaaggtc tgtcgcaggg ttctggcgtc gcgtttgata
    13021 acgaaaaatt tgcctacaat atcaacaaca acggtcacat gctgcgtatc ctcgaccacg
    13081 gtgcgatctc catgggcgac aactgggaca tgatgtacgt gggtatgtac caggatatca
    13141 actgggataa cgacaacggc accaagtggt ggaccgtcgg tattcgcccg atgtacaagt
    13201 ggacgccaat catgagcacc gtgatggaaa tcggctacga caacgtcgaa tcccagcgca
    13261 ccggcgacaa gaacaatcag tacaaaatta ccctcgcaca acaatggcag gctggcgaca
    13321 gcatctggtc acgcccggct attcgtgtct tcgcaaccta cgccaagtgg gatgagaaat
    13381 ggggttacga ctacaacggc gatagcaagg ttaacccgaa ctacggcaaa gccgttcctg
    13441 ctgatttcaa cggcggcagc ttcggtcgtg gcgacagcga cgagtggacc ttcggtgccc
    13501 agatggaaat ctggtggtaa tagcaaaacc tgggccggat aaggcgttta cgccgcatcc
    13561 ggcataaaaa caggttgtca ttatctgaaa ggggcgaaag cccctctgat tatcgggttt
    13621 agcgcgctat tgcctggcta ccgctgagct ccagattttg aggtgaaaac aatgaaaatg
    13681 aataaaagtc tcatcgccct ctgtttatca gcagggttac tggcaagcgc gcctggaatt
    13741 agccttgccg atgttaacta cgtaccgcaa aacaccagcg acgcgccagc cattccatct
    13801 gccgcgctgc aacaactcac ctggacaccg gtcgatcaat ctaaaaccca gaccacccaa
    13861 ctggcgaccg gcggccaaca actgaacgtt cccggcatca gtggtccggt tgctgcgtac
    13921 agcgtcccgg caaacattgg cgaactgacc ctgacgctga ccagcgaagt gaacaaacaa
    13981 accagcgttt ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc atcagccttc
    14041 ttccccagca gttatttcac ctaccaggaa ccaggcgtga tgagtgcaga tcggctggaa
    14101 ggcgttatgc gcctgacacc ggcgttgggg cagcaaaaac tttatgttct ggtctttacc
    14161 acggaaaaag atctccagca gacgacccaa ctgctcgacc cggctaaagc ctatgccaaa
    14221 ggcgtcggta actcgatccc ggatattccc gatccggttg ctcgtcatac cagcgatggc
    14281 ttactgaaac tgaaagtcaa aacgaactcc agctccagcg tgttggtagg accactattt
    14341 ggttcttccg ctccagctcc ggttacggta ggcaacacgg cggcaccagc tgtggctgca
    14401 cctgctccgg tgccggtgaa gaaaagcgag ccgatgctca acgacacgga aagttatttt
    14461 aataccgcga tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt aaaactgctt
    14521 gatgaagctg aacgcctggg atcgacatct gcccgttcca cctttatcag cagtgtaaaa
    14581 ggcaaggggt aattacgccc cacagtgctg attttgcaac aactggtgcg tctcctggcg
    14641 cacctttttt tatgcttcct tcctgggata tgagcgattt tttatagtac ttcacttctt
    14701 cttcactaag aatgtccatt atctcaatgc cttatcagag attcttttcc tttcgccggt
    14761 agtgtctgga cgctcaggct acttttccag gttattttat ttctgtatta cagaggtttt
    14821 atgataggtc atatcacaaa ttctggcggc aataactctt tgatgaaaca tgatgtggtt
    14881 caaggaaata atatagtaga tcttgattta ctacgtaatt tcaatggggt gacaggttta
    14941 aacagagata actttattta tatcagtgat atatttttaa atataaaaca acggaacgaa
    15001 aaaatcatgc aataaatatg ttac
//
LOCUS       DAZPBM010000101        14886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPBM010000101 DAZPBM010000000
VERSION     DAZPBM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.101"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHUZQ_002763"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHUZQ_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1026414.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHUZQ_002764"
     CDS             complement(1556..1879)
                     /locus_tag="ACHUZQ_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1026415.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHUZQ_002765"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHUZQ_002765"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1026416.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHUZQ_002766"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHUZQ_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1026417.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHUZQ_002767"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHUZQ_002767"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1026418.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHUZQ_002768"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHUZQ_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1026419.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHUZQ_002769"
     CDS             7479..8516
                     /locus_tag="ACHUZQ_002769"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1026420.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHUZQ_002770"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHUZQ_002770"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1026421.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHUZQ_002771"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHUZQ_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1026422.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHUZQ_002772"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHUZQ_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1026423.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHUZQ_002773"
     CDS             10687..11568
                     /locus_tag="ACHUZQ_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1026424.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHUZQ_002774"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHUZQ_002774"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1026425.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHUZQ_002775"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHUZQ_002775"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1026426.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHUZQ_002776"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHUZQ_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR1026427.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3589 a         3767 c         3899 g         3631 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZPBM010000102        14699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPBM010000102 DAZPBM010000000
VERSION     DAZPBM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.102"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(157..861)
                     /locus_tag="ACHUZQ_002777"
     CDS             complement(157..861)
                     /locus_tag="ACHUZQ_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1026428.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(998..1450)
                     /gene="moaE"
                     /locus_tag="ACHUZQ_002778"
     CDS             complement(998..1450)
                     /gene="moaE"
                     /locus_tag="ACHUZQ_002778"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1026429.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1452..1697)
                     /gene="moaD"
                     /locus_tag="ACHUZQ_002779"
     CDS             complement(1452..1697)
                     /gene="moaD"
                     /locus_tag="ACHUZQ_002779"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1026430.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1690..2175)
                     /gene="moaC"
                     /locus_tag="ACHUZQ_002780"
     CDS             complement(1690..2175)
                     /gene="moaC"
                     /locus_tag="ACHUZQ_002780"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1026431.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2178..2690)
                     /gene="moaB"
                     /locus_tag="ACHUZQ_002781"
     CDS             complement(2178..2690)
                     /gene="moaB"
                     /locus_tag="ACHUZQ_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1026432.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2712..3701)
                     /gene="moaA"
                     /locus_tag="ACHUZQ_002782"
     CDS             complement(2712..3701)
                     /gene="moaA"
                     /locus_tag="ACHUZQ_002782"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1026433.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3689..3833)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4098..5006
                     /gene="yvcK"
                     /locus_tag="ACHUZQ_002783"
     CDS             4098..5006
                     /gene="yvcK"
                     /locus_tag="ACHUZQ_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1026434.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5198..7219)
                     /gene="uvrB"
                     /locus_tag="ACHUZQ_002784"
     CDS             complement(5198..7219)
                     /gene="uvrB"
                     /locus_tag="ACHUZQ_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1026435.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7798..8466)
                     /gene="bioD"
                     /locus_tag="ACHUZQ_002785"
     CDS             complement(7798..8466)
                     /gene="bioD"
                     /locus_tag="ACHUZQ_002785"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1026436.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            complement(8459..9214)
                     /gene="bioC"
                     /locus_tag="ACHUZQ_002786"
     CDS             complement(8459..9214)
                     /gene="bioC"
                     /locus_tag="ACHUZQ_002786"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1026437.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGALPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9201..10355)
                     /gene="bioF"
                     /locus_tag="ACHUZQ_002787"
     CDS             complement(9201..10355)
                     /gene="bioF"
                     /locus_tag="ACHUZQ_002787"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1026438.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10352..11392)
                     /gene="bioB"
                     /locus_tag="ACHUZQ_002788"
     CDS             complement(10352..11392)
                     /gene="bioB"
                     /locus_tag="ACHUZQ_002788"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1026439.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11479..12768
                     /gene="bioA"
                     /locus_tag="ACHUZQ_002789"
     CDS             11479..12768
                     /gene="bioA"
                     /locus_tag="ACHUZQ_002789"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1026440.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12827..13303
                     /locus_tag="ACHUZQ_002790"
     CDS             12827..13303
                     /locus_tag="ACHUZQ_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1026441.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13617..13697
                     /locus_tag="ACHUZQ_002791"
                     /pseudo
     CDS             13617..13697
                     /locus_tag="ACHUZQ_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13707..13793)
                     /locus_tag="ACHUZQ_002792"
                     /pseudo
     CDS             complement(13707..13793)
                     /locus_tag="ACHUZQ_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13885..>14699
                     /locus_tag="ACHUZQ_002793"
     CDS             13885..>14699
                     /locus_tag="ACHUZQ_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1026442.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
BASE COUNT         3462 a         3997 c         3664 g         3576 t
ORIGIN      
        1 ttaaaaccac gcatacaaaa tacttttgta tttatggata cggaaaacga ttccgctcgc
       61 tcataaggca tattttatac ctggtacttg ttaagtgtcg tttttagtaa gaaaaaaatc
      121 ccgacacatg gccgggatag tgatggtgaa aaagaattaa cggcggttgc cgaagatccg
      181 taacaacatc aggaacaggt tgatgaagtc cagatacaag gttaacgcgc caagaatgga
      241 atatttgcgc aggttcgacg tgtcgcgggt atcaatctgc tcacccatat ttttcaattt
      301 ctgagtgtca tacgccgtca atccgacaaa gacaatcacg ccgatgtagg taactgccca
      361 catcaatgct tcgcttttca gccagaagtt gaccagcgat gccagcacaa tgccgattaa
      421 cgccataaac agcatattgc caaagccact taaatcgcgc ttcgtggtgt aaccgtacag
      481 gctcattgcg ccgaacatcc cggcagtaac gacgaaagta ctggcgatag aagcagcggt
      541 atagacaatg aatatactgg aaagcgtaag acccgtcagc gccgaataaa gcataaagag
      601 catcgtcgtt acacctgcgc tcagcttttg aatcatcgct gataacacaa taaccaatgc
      661 taattgcgcg atgatcagac cgattaaaaa gacacggtta gtgaacaaca gctccatcac
      721 ggccgcggaa ttagccgcat accaggcaac aaatgcggtc agcaacaagc caacggtcat
      781 ccagccatag acttgcgcca tataagtttg caagccagcc cggggttgta cgattgaatc
      841 agaacgtggg aatctgtcca tgacgatctc ctgaagatat aaggaatatc ttaaggatac
      901 tgcaaaatga tgaggctgtg catcgacgca gcgtaaacgc atgtactgag cggtgaaatt
      961 gccggacgca gcggtgcctt atccggctaa caaaaaacta ccagcgtttt gccgcctgct
     1021 gatcgctctc ccgggcttca acccagcggt cgccttccgg cgtggcttcg cgcttccaga
     1081 acggtgcgcg ggttttgaga taatccataa taaactgccc ggcttcaaac gcactgctgc
     1141 gatgcgcact ggtgacaccg acaaaaacga tttcatcgcc cggccataat tccccgatgc
     1201 ggtgaatcac cgtgacgcgc cccagcggcc agcggttacg cgcttcatca acgatttctg
     1261 ccagtgcttt ttcagtcatc cccggatagt gttcgagggt taatgctttg acgctgtcac
     1321 ccaggttatg gttgcgcacc ttaccggtaa acgtgaccac tgcaccgtct tcgtcacgct
     1381 cagccagcca cgggtactct tctcctacgc tgaacggctg cgggccgaca acaattttgg
     1441 tttctgccat cttaacctcc ggttaccggc gggaagaaag ctacttcgtc gccgtcagtc
     1501 agcggatggt caaaactcac cagcgtctgg ttgacggcag ccagcaattt gccatcttcc
     1561 agcgccagcg cccagcgatc gctctgcgca gccaggtgct ggcgtaacgc ttcaacggtt
     1621 gggtaatccg cagccacttc ggttgcatct gttcccacca actcgcgcac ctgggcgaaa
     1681 aaaagaactt taatcatccg cttccacctt aaagtcaccc gacttgccgc cgctcttcgc
     1741 cagcaaacgt accggaccaa tcaccatatc tttttgcacc gctttgcaca tgtcataaat
     1801 ggtcagcgcc gccacggagg ccgcggttaa cgcttccatt tcgacaccgg ttttcccggt
     1861 caggcggcat aaggtttcta tacgcacccg attgtgctcc ggctcggcct gtaaattgac
     1921 ttcaactttg ctgagcatca gcgggtgaca cagcgggatc agatcccagg tgcgttttgc
     1981 cgcctgaata ccgacaatac gcgcagtggc aaatacgtcg cctttgtggt ggcgaccatc
     2041 aataatcatc gccagcgtct cgctgcgcat ggtgacaaag gcttccgccc gcgcttcacg
     2101 cacggtttcc gctttggcgg agacatccac catgtgcgct tcgccagcgg cgttgatatg
     2161 ggtcagttgc gacatactta tttcttcaaa tgtggatgga aattacacgg acgcgtacgg
     2221 gcatccagct gcggcgcgat gatattttcc catgcggtac ggcacgcttt ggtcgaaccc
     2281 ggcatggcga aaatcagcgt tttgttggcg acgcccgcta ccgcacgaga ttgcaacgtg
     2341 gaagtgccaa tctcttcaaa cgacaacatc cggaacactt caccaaaacc ttcaacttca
     2401 cggtcgaaca acggcagcaa tgcttcggga gcctgatcac cttccgtcag gccagtcccc
     2461 cccgtaatca acaccacttg tacatcgtcg ctggcgatcc acgcagatac ctgagcgcga
     2521 atagcgtagc ggttttcttt cacaatggct ttatcgacaa cgtgatggcc cgcttcttgc
     2581 gccgaatcgc gcaaatagtg accggaggta tcgtcttctt caccgcgacg attagaaacc
     2641 gtaagaatag caatacgggt cgggataaat tcagtgctta cctgactcat ctgatctctc
     2701 cttttgacgt tttagccgcc aatgtacgat aagttttgcg taataccggt gttgttttga
     2761 tgcaggaaat gggtctgttt cttctcccgc agcgccgctg aaatacgcgc ttccagcgcc
     2821 tgttgctggg tatcgtcttc cagcagatcg cgcaggttaa cgccgccttc accaaacagg
     2881 cagagatgga gtttaccaat ggaggaaacg cgcaggcggt tgcaagtggc gcagaagtct
     2941 ttttcatacg gcatgataag gccaatctct ccggcgtaat ctggatggca aaagacttgc
     3001 gcgggaccgt cgctgcgttg acgtaattgg tggatccagc cgcgacgcag tagctcgtca
     3061 cgcagaacct gaccagagat gtgatgctta cggaagagct cactgccctc gcccgtttcc
     3121 atcagttcga tgaaacgcag ctggataggg cgatgctgga tccagttcag aaaggtgtcg
     3181 agctggtgat gattaacatc acgcatcagc acggtattga ctttgacctt ctcaaaaccg
     3241 acctcaaatg cagcatcgat ccctgccatg acctggttga atttatcctg cccggtaata
     3301 gcgtgaaact ggcgggcgtc cagactgtcg acactgacgt taatgccagt aagtcccgca
     3361 tcgcgccagt tcgccacatc gcgttccaga cggtaaccat tggtggtgac cgcaatctgg
     3421 cggatagcgt cgttttcccg cacagcggcg atgatatcgg taaagtcgcg gcgtaaagac
     3481 ggctctcctc ctgtcaggcg cactttttcg gtgcccagac tggcgaaggc gcgcgtaacc
     3541 cggcgaattt catcgacggt aagaaagcct ttattggtga cgccgctcgg tttgtagcca
     3601 tccggcaggc agtaggtgca acgaaagtta cacacatcgg taatcgacag gcgcaagtag
     3661 taaaacttac gcgcaaatgc atcagtcagt tgtgaagcca tgtacacctt tccagatacg
     3721 ggaggcgaag tcatttcttc cttcgccctg gtggcaatct ttacgagcat tgtcacggcc
     3781 aaagcaccgt atcagttgac ccaggtgcag aggctagagt gtttagtggt taagccgata
     3841 ctagcgtgta aatgagattt ttaccatcca cactttcgct atgtaatcat gtatatagcg
     3901 tcatgatcgt gctatttcgc tacagaataa gggaaaatgg cagttttact ttgatataca
     3961 tcatcaggga ggcatagcgt gcatcgtcct ttagaggtag aagaaaatag tttgtatcct
     4021 caatattggc aggttaattg ctgtttcccc gcaatttgcg ctactgtagc gcggataatc
     4081 tgactccagg aaactatatg cgcaatcgta cgctggctga tcttgatcgt gtcgttgctc
     4141 tcggcggagg gcatggactg ggacgcgttc tctcatcact ttcgtctttg ggttctcgtt
     4201 taacgggtat cgtcaccacc acggataatg gtggctcgac ggggcgtatt cgccgttcag
     4261 aaggcggcat tgcctggggc gatatgcgca actgcctcaa ccaactgata acggaaccga
     4321 gcgtcgcctc cgcgatgttt gaataccgtt ttggcggcaa tggcgaactt tccggtcata
     4381 atctcggaaa cttgatgtta aaggcgctgg atcaccttag cgtgcggcct ctggaagcca
     4441 tcaatttaat tcgtaatctg ctgaaagtgg atacgcattt gattccaatg tcagagcatc
     4501 ccgtcgatct gatggcgatt gacgatcagg ggcatgaagt ttacggcgag gtcaatatcg
     4561 accagttaac tacgccgatt caagagttat tattaacgcc gaatgtaccc gcaacgcgtg
     4621 aggcggttca cgctatcaat gaagcggatc tcatcattat tgggcctggc agtttttata
     4681 ccagcctgat gccaattctg ctgctgaagg aaatcgccca ggcattacgc cgcacgccag
     4741 cgccgatggt ctatatcggc aatctggggc gtgagttgag tttacctgcg gctaatttga
     4801 agctggaaag caagctggca attatggagc agtatgttgg taaaaaggtc attgatgcgg
     4861 tcatcgtcgg gccaaaagtg gatgtctcgg cggtgaaaga gcggattgtg attcaggagg
     4921 tactggaggc cagcgatatt ccgtatcgtc atgaccgcca gttgttacat aacgcgctgg
     4981 aaaaggcgtt acaggcttta ggttaacgga aatccgaaga aaaattccgg cttcctattg
     5041 aagacaaagt gcgcgttgtt tatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     5101 ccgcgctaat tcaatgtatt gcagagatca tgtaggcctg ataagcgtag cacatcaggc
     5161 aattttgctt ttgtcatctg tcttcttcgt tatcctgtca cgaagccgcg ataaacagct
     5221 cacgcagctg atgcaactgg tcacgaattt gtgccgcttc ttcgaactcc agattctgcg
     5281 cgtgttgcat cattaaccct tccagctcat ggattttctg ttgcaacgct ttaggcgaca
     5341 tatccatcgg cacattatcc ggctcaacaa tcgggcgcga ttttcctctg cccttcgctt
     5401 tggttttggc aatgttctgc cccagcgcca ggatatcgac cactttcttg ttcaagcctt
     5461 gcggcgtaat tccgtgttcc tcgttgtact tctgctgttt ctcacggcga cgttcggttt
     5521 cgccaatcgc tttcgccatt gatggggtga tcttatcgcc gtagagaatc gctttaccgt
     5581 taacgttacg tgccgcacga ccaatagtct ggatcaacga acgttcggaa cgcaggaagc
     5641 cttctttgtc agcgtcgagg atcgccacca gcgacacttc cggcatatcc agaccttcgc
     5701 gcagtaagtt gatccctacc agcacgtcga actcacccag acgcaagtcg cggataatct
     5761 ccatacgttc gacggtgtcg atatctgaat gaagataacg cacgcgctcg ccatgttctt
     5821 cgagatattc ggtaagatct tccgccatcc gcttggtcag tgtggtgacc agtacgcgtt
     5881 cgttaattgc cgctcgctga cgaatctccg aaagaagatc atcaacctgt gtcgccaccg
     5941 gccgcacttc gataatcggg tcaagcaatc cggttggacg caccacctga tccaccacat
     6001 cgccgccgga tttttccagc tcgtaattac ccggcgtcgc cgaaacatag atggtttgcg
     6061 gcgctaatgc ttcgaactct tcaaacttaa gcggacggtt atccagcgct gatggcaggc
     6121 ggaagccgta ctccaccagt gtctctttac gcgcccggtc accgcgatac atgccgccaa
     6181 tttgtggaat ggtgacgtga gattcatcga ccaccagcag cccatcggca ggcaggtaat
     6241 caaacagcgt cggcggtggc tcacccggtc cacgaccgga gaggaagcgc gagtagtttt
     6301 caatccccga acagtagccc agctcgttca tcatctccag atcaaactgg gtacgctggg
     6361 tcagccgctg ctcttccagc agtttgttgt tttccaacag cactttgcgt ctggcggcca
     6421 gttcttcttt gatctcctcc atcgcctgta cgatgcgctc gcgcggtgtg acgtagtgcg
     6481 ttttcgggta gatggtaaaa cgtggaatag tggaaacaat ctgcccggtc agcgggtcaa
     6541 ataacgacaa tcgttccact tcctcgtcaa acagttccac gcgaagtgca atgtcatccg
     6601 attctgccgg gaagatatct atcacctcgc cacgaacgcg gaaagtacca cgctggaatg
     6661 cttgatcatt acgagcgtat tgcagctccg ccagtcggcg cagaatcgcg cgctgatcga
     6721 taatcatacc gaccgtgaga tggagcatca tcttgagata taaatcagga tcgcccagac
     6781 cataaatcgc ggaaacagag gccactacaa ccacatcacg ccgctccagc atcgctttgg
     6841 tggcggataa acgcatctgc tcaatatgtt cgttaaccga ggcatctttc tcaatgaaag
     6901 tgtcggaact cggtacatag gcttccggct gatagtagtc gtagtaggag acgaaatact
     6961 ccaccgcgtt ttccgggaag aactctttca tttcgccata cagctgggcc gccagcgttt
     7021 tgttgggcgc aagtaccatg gttgggcgct gaaggtcagc aatgacattg gcaatggtga
     7081 aggttttccc tgagccagtc acgccaagta acgtctggtg cgccaggcca tcttccagcc
     7141 cctcttcgag acgtcgaatc gcctctggct gatcgccaga aggtttaaaa gcggaattca
     7201 gtttgaacgg tttactcatg agtcgctacc tgaaggagtt gggcgggcag gtatgtaatt
     7261 ttactcgtcg tacttaatta tgccaacaaa ttatactgga taaaaaaaca gttcatcacc
     7321 ataatatttc tgatacagcg taaactccgc tgtcaatcat gagcaaaatt tactctgtgg
     7381 cgagataaaa ctccggcctt accgggttat ccccaaagca acggcttttt taacatttgt
     7441 caagatgagt gatgacagtt ttatggcaag agatgcctgt tcagtgactg ccattgcatt
     7501 tatctaacca gttaaaaaat aaaagatatt tctttgagcc gtctttaacg ccaattcgcg
     7561 tgcaagccgc gtattctctc gcttgcctcg tgttttctaa ctctaataca catggttatc
     7621 cacaggaata gtggataact gtctccagcc cctatccccc gccgcctggg caattcccac
     7681 attcgccgca tgaggcggaa gtaaaatttt tttcgtgatt attttagaat ttaattggtt
     7741 aaattgcagt caatcgaaga cgcgatctcg ctcgcaattt aaccaaatac agaatggcta
     7801 caacaaggca aggtttatgt actttccggt tgccgcattt tctgcaagcc aggggatctc
     7861 tcccagcagc ggcgcgggaa tcatgcgggt gagcgtggtc atatattcag cgtgacgttt
     7921 tcccggaggc gtaacatcgt tcgccaccca acccgccaga gtcagtccgg cgtgttgtat
     7981 tgcctgcgca gtcaacatcg cgtgattaat acagccgagt ttcacaccaa ctaccagtat
     8041 caccggcagt tgttcctgtg ttacccaatc tgcaaaagtg aaagtgtcag aaagcggcgt
     8101 aaaccagccg ccagcacctt ccactaacac ccagtcagcc tgttgttcaa gcgcgcgtaa
     8161 tccggcgctc attaccaatg attctatcgg cctgccctct tgcgcgctga tgatgtgcgg
     8221 cgaagtgggt tctgcgaagg tgtaaggatt tactgttgcg taatccagct gcaggctgct
     8281 gttgtgctgt aacgccagcg cgtcactatt acgtaagcct tccggggtct tttcgctgcc
     8341 agaggcgacc ggtttataac ctgccgtccg ataacctact gccttcgcgg cttgtaaaag
     8401 tgcacaactg gcgacggttt tccccacttc ggtatccgtt ccggtgacaa aataacgttt
     8461 actcacgagc aatcactccc aaaaaaagat gatacgtcag aggatatcgc ccctgttgtt
     8521 gcggccaggc cagttgcaat cgctgcaatt gcgaacgcgt taatattcgc gggtcgcgcc
     8581 cttcatgaag atgcgtagca ccgatgcctt tcagcgaacg catggcactg agcgcatcat
     8641 caaaccatag tgtgatgggc tgaatatgat gttgataatg cacgccgttc agcgactgtt
     8701 cgatttcatc tggcggtaaa aagcgattag catgcggacg ctcgtccacc gcctgccacg
     8761 cctgatgcag ttcgggtaac gctccctgca ccagcgtggt aaacgccacc acgcctccgg
     8821 ggcgcaccac ccgatacagc tcgcggagtg ccgtggataa attaccgcac cactgcaccg
     8881 cgagattgct ccatgcaaga tcgaacgtcg cagtcgctaa cggcagggat tcgatatctc
     8941 ccgccagata atggtctgca gcatctttct ggcgggcttg cacaagcatt ggcggtgaga
     9001 gatctaacgc cgtcacctgt gcgtgacgct ctcgccagtg gcggctcatc cagcctggcc
     9061 cacaccccgc gtccagtacg tgggtgtatt tacgctgtgg gagcattgcc agtaagacgt
     9121 cagcactccg gcgctgtaac tctgcgtgtt gctcatagtg cgcggctgcc cgaccaaatg
     9181 ccgctgcaat ggcttgttta ttaaccgttg ccatgcagca cctccagcag acggtcgata
     9241 tcctgcattt catgcgctgc ggttagcgtt aagcgcagtc gcgcagtacc agcgggtacg
     9301 gttggcgggc gaatcgccgt gacccagcag ccttgctggc gcagtttttc tgccagttgt
     9361 aacgcacggc tgttatcacc gacgatcaat ggctggatgg cgctgcatga atcagcaagc
     9421 gtaaacggca aatcctgtac tccggcacga aaacgcgcaa tgagtgacac cagtttttcg
     9481 cgccgtgcat ccccctcatc acggcgaatg accgccagcg acgcacgtaa tgcctgcgcc
     9541 tgagcgggcg gcatactggt gctgtagata aggtggcggg cgaattgcag cagataatcc
     9601 gccaccgtac tggagcaaag caccgcagcc ccactgacgc caaatccttt gccaaaagtc
     9661 accaccagca attctggttt taccttttgc agccagcagc tgccgcgccc ctgctccccg
     9721 ataacgcccg tgccgtgggc atcatcgacc atcaaccagc cattgtgctg ttgcgttacc
     9781 tgctggattt ccgccagtgg cgcgctatcg ccgtccatgc tgaacacgcc ttctgtcacc
     9841 accagttgct gccccggaca gggggaagca agcagtcgcg ccagatgagc gacatcgtta
     9901 tgagtaaaac ggcgaagctg cgacgggctt aaactggcag cctccagcaa ggaggcatgg
     9961 ctaagccggt cggcaacaat acggtcctct ttcgccatca tcgcggcaat gactgcctga
    10021 ttcgcggcaa aaccagagat aaacagcagc gcccgcgaat agccaagcca ctcggccagt
    10081 tcttcttcca gcgcctgatg caccacgcta taaccgctga catgcccgga accgccgcta
    10141 cctacaccaa acttttctgc gccctgctgc caggcgcgga taatttgcgg atgatggctt
    10201 aaacctaaat aatcgttact ggaaaagttc agatactggc gatcatccgc caccagccag
    10261 cgtccggctc cttgcgccac cggataacgg cgacgcaggg catcggcagt acgccgtgca
    10321 tcgagcgccg cgttgatttt ctcctgccag ctcataatgc tgccgcgttg taatattcgt
    10381 cggtgtccgg ggtcatcagc gcctgttcaa gacgttgctg ttgttcgtta tcccctgcca
    10441 gcacggcagt ttgctgcgga tttagcccca gtttgcggaa cagttgcagg tctttatctt
    10501 cttccggatt cggcgtggtc agcagtttgc aaccgtagaa aatcgagttt gcgccagcca
    10561 taaagcacat cgcctgagtc tgttcattca tctgctcgcg tccggcagaa aggcgcacgt
    10621 aagaggtcgg catcatgatc cgcgctaccg caatagtgcg aataaaatca aaggcatcga
    10681 catcatcgtt atccgccagt ggcgtgcctt tcaccttcac cagcatgttg attggtacgc
    10741 tttccggcgg cgtcggcagg tttgccagtt gcagcaataa tccggcgcga tcttttaccg
    10801 tttcgcctaa gccaacaatg ccgccagaac agactttgat cccggcatcg cgcacttttt
    10861 ccagcgtatc gaggcgttcc tgataagtgc gggtggtgat gatattgccg taaaactccg
    10921 gcgaggtgtc caggttgtgg ttgtaataat ccagcccggc gttcgcgagg cgctgcgcct
    10981 gagattcact caacgtgccc agcgtcatac acgcctccag ccccatcgct tttaccccct
    11041 gcaccatttg ttccaggtac ggcatatcgc gttcgtgggg attcttccac gccgcgccca
    11101 tacagaagcg cgtcgatcct gccgctttcg ctttgcgcgc cgactccagc acctgttcaa
    11161 cttccatcaa ccgctcggct tccagcccgg ttttgtagcg tgagctttgc gggcagtatt
    11221 tgcaatcttc cggacaagct ccggtcttaa tcgacagcaa cgtgctgacc tggacctgac
    11281 gaggatcgaa atgctggcga tggacctgct gcgcttcaaa cagcagatcc agcaacggtt
    11341 tttcaaataa ttctgtgact tgcgacaagg tccagcgtgg gcggtgagcc atggggcttc
    11401 tccaaaacgt gttttttgtt gttaattcgg tgtagacttg taaacctaaa tcttttcaat
    11461 ttggtttaca agtcgattat gacaacggac gatcttgcct ttgaccaacg ccatatctgg
    11521 cacccataca catccatgac ctccccgctg ccggtttatc cggtggtgag cgccgaaggt
    11581 tgcgagctga ttttgtctga cggcagacgc ctggttgacg gtatgtcgtc ctggtgggcg
    11641 gcgatccacg gctacaatca cccgcagctt aatgcggcga tgaagtcgca aattgatgcc
    11701 atgtcgcatg tgatgtttgg cggtatcacc catgcgccag ccattgagct gtgccgcaaa
    11761 ctggtggcga tgacgccgca accgctggag tgcgtttttc tcgcggactc cggttccgta
    11821 gcggtggaag tggcgatgaa aatggcgttg cagtactggc aagccaaagg cgaagcgcgc
    11881 cagcgttttc tgaccttccg caatggttat catggcgata cctttggcgc gatgtcggtg
    11941 tgcgatccgg acaactcaat gcacagcctg tggaaaggct atctgccaga aaacttgttt
    12001 gctcccgccc cacaaagccg tatggatggc gaatgggatg agcgcgatat ggtgggcttt
    12061 gcccgcctga tggcggcgca tcgtcatgaa atcgcggcgg tgatcattga gccgattgtc
    12121 cagggcgcag gcgggatgcg catgtaccat ccggaatggt taaaacgaat ccgcaaaata
    12181 tgcgatcgcg aaggtatctt gctgattgcc gacgagatcg ccactggatt tggtcgtacc
    12241 gggaaactgt ttgcctgtga acatgcagaa atcgcgccgg acattttgtg cctcggtaaa
    12301 gccttaaccg gcggcacaat gaccctttcc gccacactca ccacgcgcga ggttgcagaa
    12361 accatcagta acggtgaagc cggttgcttt atgcatgggc caacttttat gggcaatccg
    12421 ctggcctgcg cggcagcaaa cgccagcctg gcgattctcg aatctggcga ctggcagcaa
    12481 caggtggcgg atattgaagt acagctgcgc gagcaacttg cccccgcccg tgatgccgaa
    12541 atggttgccg atgtgcgcgt actgggggcc attggcgtgg tcgaaaccac tcatccggtg
    12601 aatatggcgg cgctgcaaaa attctttgtc gaacagggtg tctggatccg gccttttggc
    12661 aaactgattt acctgatgcc gccctatatt attctcccgc aacagttgca gcgtctgacc
    12721 gcagcggtta accgcgcggt acaggatgaa acattttttt gccaataacg agaagtccgc
    12781 gtgagggttt ctggctacac tttctgcaaa caagaaagga gggttcatga aactcatcag
    12841 taacgatctg cgcgatggcg ataaattgcc gcatcgtcat gtctttaacg gcatgggtta
    12901 cgatggcgat aatatttcac cgcatctggc gtgggatgat gttcctgcgg gaacgaaaag
    12961 ttttgttgtc acctgctacg acccggatgc gccaaccggc tccggctggt ggcactgggt
    13021 agttgttaac ttacccgctg atacccgcgt attaccgcaa gggtttggct ctggtctggt
    13081 agcaatgcca gacggcgttt tgcagacgcg taccgacttt ggtaaaaccg ggtacgatgg
    13141 cgcagcaccg ccgaaaggcg aaactcatcg ctacattttt accgttcacg cgctggatat
    13201 agaacgtatt gatgtcgatg aaggtgccag cggcgcgatg gtcgggttta acgttcattt
    13261 ccactctctg gcaagcgcct cgattactgc gatgtttagt taatcactct gccagatggc
    13321 gcaatgccat ctggtatcac ttaaaggtat taaaaacaac tttttgtctt tttaccttcc
    13381 cgtttcgctc aagttagtat aaaaagctga acgagaaaca gtaaaaatca ttaatatcaa
    13441 tgcattataa tacatttagc ttaaaaaata gactgcataa tactgcaaaa cacaacatat
    13501 ccagtcacta tgaatcaacc acttagatgg tattagtgac ctgatacaga gcattagcgc
    13561 aaggtgattt tttgtcctct tgcgctaatt ttttgtcaac gcactgggtt agctgaattt
    13621 acaaccatgc tcagtatctc gataagcgca gagaaatgat gcagtggtgg gcagactact
    13681 ggcttgatga aaaggtaggg cgaccaaccg cgttttttca gttcagtcag cacattgagc
    13741 cagaacttca ccccttcatt ttcggccagc cacatcccca acagttcttc ctgttcttaa
    13801 ctatttacag gcgcgttttt ttaaagaatc ctcactccat ttgcacgaac atattaatat
    13861 ataaacaatg aggatcaata ataaatgaaa cctgcccaca atccttcttt tttccgctcc
    13921 ttttgtggtt taggatgtat atcccgttta tccgtagaag agcaaaatat cacggattat
    13981 caccgcatct gggataactg ggccaaggaa ggtgctgcaa cagaagaccg aacacaggca
    14041 gttcgattac tgaaaatatg tctggctttt caagagccag ccctcaattt aagtttactc
    14101 agattacgct ctctcccata cctgcccccg cacatacaag aacttaacat ctctagcaat
    14161 gagctacgct ctctgccaga actccctccg tccttaactg tacttaaagc cagcgataac
    14221 agactgagca ggctcccggc tcttccgcct cacctggtcg ctcttgatgt ttcacttaac
    14281 agagttttaa catgtttgcc ttctcttcca tcttccttgc agtcactctc agcccttctc
    14341 aatagcctgg agacgctacc tgatcttccc ccggctctac aaaaactttc tgttggcaac
    14401 aaccagctta ctgccttacc agaattacca tgtgaactac aggaactaag tgcttttgat
    14461 aacagattac aagagctacc gccccttcct caaaatctga ggcttttaaa cgttggggaa
    14521 aaccaactac acagactgcc cgaacttcca caacgtctgc aatcactata tatccctaac
    14581 aatcagctga acacattgcc agacagtatc atgaatctgc acatttatgc agatgttaat
    14641 atttataaca atccattgtc gactcgcact ctgcaagccc tgcaaagatt aacctcttc
//
LOCUS       DAZPBM010000103        14339 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPBM010000103 DAZPBM010000000
VERSION     DAZPBM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.103"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            226..573
                     /locus_tag="ACHUZQ_002794"
                     /pseudo
     CDS             226..573
                     /locus_tag="ACHUZQ_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            626..1021
                     /locus_tag="ACHUZQ_002795"
     CDS             626..1021
                     /locus_tag="ACHUZQ_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1026443.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1062..1805
                     /gene="cmoA"
                     /locus_tag="ACHUZQ_002796"
     CDS             1062..1805
                     /gene="cmoA"
                     /locus_tag="ACHUZQ_002796"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1026444.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1802..2773
                     /gene="cmoB"
                     /locus_tag="ACHUZQ_002797"
     CDS             1802..2773
                     /gene="cmoB"
                     /locus_tag="ACHUZQ_002797"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1026445.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2938..5367)
                     /gene="torZ"
                     /locus_tag="ACHUZQ_002798"
     CDS             complement(2938..5367)
                     /gene="torZ"
                     /locus_tag="ACHUZQ_002798"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1026446.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5392..6492)
                     /locus_tag="ACHUZQ_002799"
     CDS             complement(5392..6492)
                     /locus_tag="ACHUZQ_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1026447.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(6880..7626)
                     /gene="cutC"
                     /locus_tag="ACHUZQ_002800"
     CDS             complement(6880..7626)
                     /gene="cutC"
                     /locus_tag="ACHUZQ_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1026448.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7640..8206)
                     /locus_tag="ACHUZQ_002801"
     CDS             complement(7640..8206)
                     /locus_tag="ACHUZQ_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1026449.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8422..10158
                     /gene="argS"
                     /locus_tag="ACHUZQ_002802"
     CDS             8422..10158
                     /gene="argS"
                     /locus_tag="ACHUZQ_002802"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1026450.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(10200..10244)
                     /gene="yecV"
                     /locus_tag="ACHUZQ_002803"
     CDS             complement(10200..10244)
                     /gene="yecV"
                     /locus_tag="ACHUZQ_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR1026451.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            10335..10823
                     /locus_tag="ACHUZQ_002804"
     CDS             10335..10823
                     /locus_tag="ACHUZQ_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1026452.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10943..11335)
                     /gene="flhe"
                     /locus_tag="ACHUZQ_002805"
     CDS             complement(10943..11335)
                     /gene="flhe"
                     /locus_tag="ACHUZQ_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1026453.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(11335..13413)
                     /gene="flhA"
                     /locus_tag="ACHUZQ_002806"
     CDS             complement(11335..13413)
                     /gene="flhA"
                     /locus_tag="ACHUZQ_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR1026454.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(13406..>14339)
                     /gene="flhB"
                     /locus_tag="ACHUZQ_002807"
     CDS             complement(13406..>14339)
                     /gene="flhB"
                     /locus_tag="ACHUZQ_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR1026455.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         3502 a         3791 c         3446 g         3600 t
ORIGIN      
        1 tcaataagtt atgctaaaaa gagactatac cgatactgca tagaatttgt gtaggattgc
       61 atagatactt ggaaaaacac cgttaaattg catagtcatt cacctgatat tgtcctgttt
      121 ttacaggtag ttgattcgga tgttctagga atcatcctca tagttgattc ggttagttgg
      181 gcttgtacca acccctaatc aacctcatag tgtatttaga ccttgatgat tttagacagc
      241 cgccctgttc atgcagcacg tccacacagt gaagctattc gcgacgctca acgaaaaaaa
      301 cctaaagttc cggtacatgc tgtactgacg gcgacaaatc cgctgattcg ttttatcggt
      361 agtgatgata tgacgcaaaa ccgggaatta tttcaggtct ggttacagaa attagcgcag
      421 tggcatcaga ccactacgcc ttatcttttt ttacatacgc cagacattgc ccaggccccg
      481 gaactggtac ataccctgtg ggaagactta cgtaaaacgc ttccagagat cggagcagtt
      541 ccggctattc cacagcaatc ttctcttttc tgaatttgcc acctatcata gacaggtgcc
      601 atcggccatt ttaaagggag tttgtatggt aagcgcgcta tatgccgttt taagtgcgtt
      661 gttattaatg aagttctctt ttgatgtcgt tcgcctgcga atgcagtacc gcgttgccta
      721 tggtgacggt ggttttagcg aactgcaaag cgctattcgc attcatggta acgcggtgga
      781 atatattccc atcgcgattg tgctgatgct gtttatggaa atgaatggcg cagaaacctg
      841 gatggtgcat atttgcggca tcgttttgct tgctggtcgt ctgatgcatt attacggttt
      901 tcatcaccgt ctgtttcgct ggcgacgttc cggcatgagc gccacctggt gtgcgctgtt
      961 gctgatggtg ctggcgaatc tttggtatat gccctgggag ttggttttct ccctgcgtta
     1021 gcgcacaata cgccactttc tttttcccgg atttttacgt tatgtctcac cgcgacacgc
     1081 tattttctgc ccctatcgcc agactgggcg actggacctt tgatgaacgg gtagctgaag
     1141 tcttcccgga tatgatccag cgttccgttc ccggctattc caatattatt tccatgattg
     1201 gtatgttagc cgagcgcttc gttcaacctg gtacgcaggt ttacgatctg ggttgttctc
     1261 tgggcgcggc gacgctctcg gtgcgtcgca acattcatca tgataattgc aaaattattg
     1321 ccatcgacaa ctccccggcg atgattgaac gctgcagtcg tcatattgac gcctataaag
     1381 cccctacgcc agtagacgtc attgaaggtg atattcgcga tatcgccatt gaaaacgcat
     1441 cgatggtggt gctgaatttt accctgcaat tcctggaacc ttccgagcgc caggcattac
     1501 tggataaaat ttatcaagga ctgaacccgg gcggcgcgct ggtgctttcg gaaaaattca
     1561 gtttcgaaga tgccaaagtt ggtgaactgt tgttcaacat gcaccacgac tttaaacgtg
     1621 ccaacggtta cagcgaactg gagatcagcc agaagcgcag catgctggaa aacgtgatgc
     1681 tgaccgattc cgtggaaacc cataaagcac gcctgcataa agccggtttt gagcatagcg
     1741 agctgtggtt ccagtgcttt aactttggtt cactggtggc attaaaagca gaggacgctg
     1801 catgatcgac tttggtaact tttattctct gattgccaaa aatcatcttt cacactggct
     1861 cgaaacgctg cccgcgcaga ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa
     1921 gcagtggtcc aacgcggtgg aatttctgcc tgaaattaaa ccgtatcgtc tggatttatt
     1981 gcatagcgta accgccgaaa gcgaagagcc actgagcacc gggcaaatta aacgcattga
     2041 aacgcttatg cggaacctga tgccatggcg caaagggccg ttctcactgt atggcgtcaa
     2101 catcgatacc gaatggcgtt ccgactggaa atgggatcgt gttctgcccc atctttctga
     2161 tttaaccgga cgcaccattc tggatgtcgg ctgcggcagc ggttatcaca tgtggcgcat
     2221 gattggcgca ggcgcgcacc tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca
     2281 gtttgaagcg gtgcgtaaac tactgggtaa cgatcagcgc gcacatttgt taccgttagg
     2341 tattgaacag cttccggcac tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta
     2401 ccatcgccgt tcgccgctgg agcatctctg gcagttgaaa gatcaactgg tgaatgaggg
     2461 tgaactggtg ctggaaacgc tggttattga tggcgatgag aacacggtgc tggtaccagg
     2521 cgatcgttac gcgcaaatgc gtaatgtcta tttcatccct tccgcgctgg cgctgaaaaa
     2581 ctggctgaag aagtgtggtt ttgtagatat ccgcattgtg gatgtgtgcg ttaccactac
     2641 tgaagagcag cgacgcactg aatggatggt taccgaatct ctgtccgatt tcctcgaccc
     2701 acatgatccg agtaagacag ttgaaggtta tcctgcgcct aaacgcgcgg tgctgattgc
     2761 gcgcaagccg taaaggtctg gtattactgc cggatgcggc gtgaatgcct tatccggcct
     2821 acaaaccctt gctaattcaa tctattgcag ggactatgta ggcctgataa gcatagcgca
     2881 tcaggcagtt ttacgtttgc ataacctcag cgcccgtttc cgggcgctat tcacttctta
     2941 cgcctgaaca gcaggcttat caaatgccgt taacttcggt gcgttacccg tatatttttc
     3001 gatatacacc agcgcagagt taccggcaca ggcatttgcc agctgcgagc tggggatatc
     3061 cgccgttaac acgttcgcac tgccgttttt acacaagccg ttttccagat ctggccatgc
     3121 accttcatgc acgcatacca cgcctttttt gatcccgtca gtcaccaccg cgcctgtcaa
     3181 aatctgacca cgtttgttcc agacacgcac cagatcgcca ttcgcaatac caaatcgagc
     3241 agcatcttcg gtgtgaatag tgatcggttc acgatcggca accgcatatt ttttacgcag
     3301 ttccgcatag ttaagctgac tgtgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa
     3361 ctgcttctcg tcggcggtac ccttccactc atcaggcgca agccaggttg ggtgtgccgg
     3421 gcaatcctta tagccaaatt tttccagcgt tttggagtaa atctcaattt tgccacttgg
     3481 cgtacccagc gcatttttca ccggatcggc gcggaaatca ccataacgaa cgtactgttc
     3541 gttcttctcg ctgcggcgca tttcgatcag tttattttgc tgccagaagg cattaaacat
     3601 tggcatagtg acgcgttgcg cacgggcccc tttctgagcg gcatcataga aaaatttcag
     3661 ccacgccatt tcatctttac cttcggtata gatctctttt ccgccaggtt tgagtaattc
     3721 cgcaagatcg gcaaacacat caaaatcgtt tcgcgcttca aattgcggag caacagcctg
     3781 cttcatcggc acaatatgct ggttgctgta atcaccggtc atcgtcaagt cattgcgctc
     3841 aaacgatgtg gtgatcggta atacgatatc tgcgtgttta gcagctgcgg tccagtagca
     3901 ttcagaaacg acgatcatct ccggtttctg ccatgcttta atcagacgat tagtatcctg
     3961 gtgatgggta aagttaccgc cacccgccca ccagatcatt ttgatattcg gatacgtttg
     4021 ctctttaccg ttatgctgat atttgccgcc aggattttcc agcgcatcga cgatacgggc
     4081 aacaggaatc gccctcattc cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc
     4141 cgcagacatt tccggcagca cgccgccaac acgtgtcggg ttgccaccat tggagtaatg
     4201 ataagagaaa ccaaaaccgc cgcccggtgt accaatctgc cctaacatcg ccgccagcgt
     4261 caccagcatc cagtgtttct gttcaccata ttgctggcgc tgaattcccc agcctgccat
     4321 cagcattgtg cgattcgccg ccatcagttc agcgagtttg actatctgcg cttccggtac
     4381 gccagtaatt tccgccgccc aggccgcgct cttcggcgta ttatcgctct tacctctcag
     4441 atactcttca aactgcggat acccggtagt gtatttttca aggaacactt tatcgtgttt
     4501 gccttgtgtc atcagggtat gcgcaatccc taacatcagt gccacgtcgg tgcccatatt
     4561 cggcgcgatc caggtggcat tatcgccaaa gaagtcgatg gtttcggagc ggataggatc
     4621 aatggcaatc actggtttgc cagatttttt cagctgatgg aagtattcca gcccttgctc
     4681 atcggtactg ctccaggcaa tttttaaggt attcagcggg ttcatccccc acagcaccac
     4741 aacctggctg ttttccagaa tcagcggcca ggaggtctgc tgttcataca cctctacaga
     4801 accgaccaca tgcggcatga tcacctgtgc cgccccggtt gaataatcgc cgctatgccc
     4861 ggaataaccg cccgccaggt tcatataacg ttgcagtaag gtttgcgctt tatgcaacac
     4921 gccagaagag cgccagccgt atgatccggc aaaaatagcc gatggtccgt tagctttgcg
     4981 aatgcgatcg tgttgctcat gaatcagttt taatgcttgt tcccagctca cctgtacata
     5041 ggtatcttcg ccacgacctt tcgtcggttg cagtggatta tcgagatagc tttttctcac
     5101 catcggatgc tgaatacgcg tcgtggtgtg tacctgatcc gccgccgtag actgtaagga
     5161 attcggtatg gttttcgcca gcgcgcctgt tgaagaaaca atcttgccgt ccttcacttc
     5221 tacgttcatc gctccccaac gtcccgcggt gaggatttta ccgtccttct cttctgccca
     5281 tgcggggagc ggtgctgccg atgtcaccac cagcgctcca gcggcaatac cgctgtgttt
     5341 aataaattca cgtcttgtta atgtcataac ttcctccctg atcaacgagg atcactgttt
     5401 ctcggtaata tctttggcgt tgtactggaa ataccgcgtt aaaatgtcca gttcgttttc
     5461 gctcatgctg gttcgtgccc ccattccttt ggcaatggac ggccacgcat tgacggtgta
     5521 atggtcggcg gcaatagggg catgacaacc agcgcaatag gtatcgtcaa gtttttcagc
     5581 gtattgccat agcggtttac ggtccgctaa tccgggatcg gtaagcgcac cctgtaaaga
     5641 cgcctgacgc cattgattgc cgtattcgtc agcctgccat tccccgttta ccgtgagcgc
     5701 cttgatacct tcttcactta atgtggctag cgccagccgt tgacctgccg ccaggtagag
     5761 cgtgttttca ctgccctgca tttgataacc ctgcaacaga acgatcggct gtttgccact
     5821 ggcatcaacg acggtgagat cggtaccagg attcacggta gccagctcgc ctatgcgaga
     5881 agttttgaaa ggataaatat gtgcgccatt agtcactgaa gtagcggcct gactttccag
     5941 ctcatgcgcc gcgttgtcat ccatttttat ttctggcgga aaatgggcaa tgcctttatg
     6001 acaatcgata caggtttcgc cgcccttttg tgctttgtta tgcattttct gcgcagattc
     6061 actttgcgag gcaatatcca tggcatcaaa agaatggcaa ctacggcacg ttgcagagtc
     6121 agttgctttt aattctttcc atactgtttc ggccatttcc tggcgatgag tttcgaactt
     6181 atcgtcactg tctattttgc cgctaacaaa ttcatgataa atatctttag atgcttttaa
     6241 tttagcaaat aaataatcca tccctgactt tggaatatgg caatctgcac attccgcacg
     6301 tatccctttc tggttcgaaa agtggacagt tccctgatat tcctcaaaag gtttactcat
     6361 cgagtggcag gaaaggcaaa atgctgtatc cgacgtttta tgtaaggctt tttgcgccag
     6421 caataacccg ccgccaccaa ccacaaccgc tatcagcaaa aacaataacc caatgcgttt
     6481 tttccctcgc atagattcct ctaaataaat tccagaacac cgaaatcatt gtataattaa
     6541 atacacaacg aattaaccgc ttgcataact cggcacaaca ctgcctgaaa taatcgataa
     6601 agaatatgat ttattacaat gtaatcatta attgctaagg aataacccag ttgctattta
     6661 gaatatatat ttatcagttt tagcaattta aatcccataa tcaatgtgaa tataaaaaat
     6721 attattagca caacgctatg ttcactgtca cccgctcaac agatcacggt tatcgttcgt
     6781 tttttatact gctcagggat aaaaaaaggc ccctgttgaa attgcagggg cctggtacga
     6841 gcaagcatca tattgggcga catgatgcaa cggtaaaaat catttggcct gatggcgttc
     6901 aatgattcct ttcatttcag caaccgccgc cccgtctacg atataacgcg aatactcgtc
     6961 cgcgtgttca tctgatgaca tggacaatcc ttgattacga taacgcatcg gtgaggcttg
     7021 ccacgctccc gcggagctat ggacttccag cactccggca tcgaggaagt ggtgcaggtt
     7081 ttctgcacgg actcctgctc cggccataat gattggagca tcacgatggg caataagttc
     7141 cataattttt gataaacctt gcagtgcgtc tgatttttgc cctgatgtca gtacccgggc
     7201 aatgcccaat tccgcaagat tattgagtgt atataaaggg ttagcgcaca tatcgaaggc
     7261 gcgatgaaaa gtcactgcca gcggaccggc agcagccatt attttttcca ttcgtggcat
     7321 atcgacattc ccgtcaacat cgagaacgcc cgtcaccagt ccaggaaaac ctaattcgcg
     7381 gaccgtgcgc acatcctcaa gaatggcggc aaactcaccg tcgctgtagc aaaaatcacc
     7441 accgcgtggg cgaattatcg gatgcacagg gatcgtcacc cgctggcgca cggatttcag
     7501 tacccccaac gacggcgtta agcccccctc ttttggggct gcgcataatt caactctgtc
     7561 tgcgccgttt tgctgcgccg ttagtgcaca ttccatgctg taacagcaaa tttccagtaa
     7621 tgccattttt gctccttaat tacgctgact gctcgctggc aacgatctct tcaatggacc
     7681 acggatgaaa cttaatagtt gttttgccat cggtgaccgc cagcgtcgga ttcggtaatc
     7741 gctcatgctc accttttggt gaactggttt taactgagat ccccggtagg ctgagtcctt
     7801 catcagaaag caatgccagc gcacgggcat tcagcgtttc tggatcaccc agcagaacga
     7861 tttcaatatg ttcccaacct tcgtgtgggt aacgtttttc cccgggccac ggtagctcca
     7921 caatagaaaa ctgccagtgc gcaacctgta ccggttcatg caatttaaac agacaaatcg
     7981 gtctgccatt gatcatattt tctgacaaaa gctcgccaca ctgttcaaac ccgcgacgcc
     8041 agcgttcagc agtggcgttt tgatggcaac gcaaagaaat gtgatcggca gtcagcggag
     8101 tgatattcaa ccccagacgg agggaaagtt catctaatgc gtgggtaaat cgcggtaaat
     8161 ccgatgcaat atcctgcagc tcgtcgatag attgccagtt cgccataatc actcttcgtc
     8221 tttcagtaaa agcgttaatt taccctgttg ccctgcgcca accaaccgct gatttcacgc
     8281 cgcttctgat gcaatagtga aaacggcaat acgccacgcg cacgttgctg acgaaaacag
     8341 ccatttgcag tatactcccg ccctaatttc tttaactggt gcgggcaatt tttgctcgct
     8401 tcatcaatgt aaggtattcc ggtgaatatt caggctcttc tctcagaaaa agtccgtcag
     8461 gccatgattg cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca ttcagcaaaa
     8521 gttcagttcg gcgactatca ggctaacggc atgatggcag ttgctaaaaa actgggtatg
     8581 acaccgcgac aattagcaga gcaggtgctg actcatctgg atcttaacgg tatcgccagc
     8641 aaagttgaga tcgccggtcc aggctttatc aacattttcc ttgatccggc attcctggct
     8701 gaacatgttc agcaggcgct ggcgtccgat cgtctcggtg tttctacgcc agaaaaacag
     8761 accattgtgg ttgactactc tgcgccaaac gtggcgaaag agatgcatgt cggtcacctg
     8821 cgctctacca ttattggtga cgcagcagtg cgtactctgg agttcctcgg tcacaaagtg
     8881 attcgcgcaa accacgtcgg cgactggggc actcagttcg gtatgctgat tgcatggctg
     8941 gaaaagcagc agcaggaaaa cgccggtgat gaaatggagc tggctgacct tgaaggtttc
     9001 taccgcgatg cgaaaaagca ttacgacgaa gatgaagagt ttgccgagcg tgcacgtaac
     9061 tacgtggtaa aactgcaaag cggtgacgaa tatttccgcg agatgtggcg caaactggtc
     9121 gacatcacca tgacgcagaa ccagatcacc tacgatcgtc tcaacgtgac gctgacccgt
     9181 gatgacgtga tgggcgaaag cctctacaac ccaatgctgc caggaattgt ggcggatctc
     9241 aaagccaaag gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct tgatgagttt
     9301 aaaaacaagg aaggcgaacc gatgggcgtg atcattcaga agaaagatgg cggctatctc
     9361 tacaccacca ctgatatcgc ctgtgcgaaa tatcgttatg aaacactgca tgccgatcgc
     9421 gtgctgtatt acatcgactc ccgtcagcat caacacctga tgcaggcatg ggcgatcgtc
     9481 cgtaaagcag gctatgtacc ggaatccgta ccgctggaac accacatgtt cggcatgatg
     9541 ctgggtaaag acggcaaacc gttcaaaacc cgcgcgggtg gtacagtgaa actggccgat
     9601 ctgctggatg aagccctgga acgtgcacgc cgtctggtgg cagaaaagaa cccggatatg
     9661 ccagctgacg agctggaaaa actggctaac gcggttggta ttggtgcggt gaaatatgcg
     9721 gatctctcca aaaaccgcac cacggactac atcttcgact gggacaacat gctggcgttt
     9781 gagggtaata ccgcgccata catgcagtat gcttacacgc gtgtattgtc cgtgttccgt
     9841 aaggcagaaa ttaacgaaga gcaactggct gcagctccgg taatcatccg tgaagatcgt
     9901 gaagcgcaac tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt ggttgcccgt
     9961 gaaggcacgc cgcatgtgat gtgtgcttac ctgtacgatc tggctggtct gttctctggc
    10021 ttctacgagc actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa cagccgtctg
    10081 aaactggcac aactgacagc gaagacgcta aagctgggtc tggatacgct gggtattgag
    10141 acagtagagc gtatgtaatc gatttttcgt gagagtaaag cctgatcagg gttacctgat
    10201 caggcttttt tattgccatc taaatgtatt ctgaaaatgg acatgccatt gttttctgac
    10261 tgttggataa gaggccagaa gcgtaatatc cggccccagg gaaacgataa cggttgaatt
    10321 taaggaatac cgcagtgttt aaatttcttg tattaacatt aggcattatc tcttgccagg
    10381 cttacgcaga agatacggtt atagaaaacg accatgacat ttcagccatc aaagattgtt
    10441 ggcaaaaaaa ttcagatgat gatactgaca ttaacgtgat caaatcatgc ctgcgacaag
    10501 aatacaatct cgtcgatgcg caattaaata aagcctatgg tgaagcttat cgttatatag
    10561 aacaagtgcc acgcacaggt gtaaaaaaac ctgataccga acaacttaac ttgcttaaaa
    10621 aatcacagcg agcctggctg gattttaggg acaaagaatg tgaattaatc ctttcaaatg
    10681 aggacgttca ggatttaagt gacccttatt ctgaatcaga atggctctca tgtatgatca
    10741 tacagaccaa tacgcgaact cgccagttgc agctataccg taactctgaa gatttttatc
    10801 caagcccttt gacaagagga taattcacat ctttttggca tgttttgttg caagctattc
    10861 ctgataaata attgcaacaa gacatcgagc ctttttcact gagttattaa acatactcgc
    10921 gagcgcgtaa tttttttgtc cttcagcgat aattcacaat cacttcatta cgttgtacct
    10981 ttagcggtgg aattaaccgc ccaccgcctg gcacttccca gataaatcgc aacggttctg
    11041 ctgccgctat accggaaaag gccacggtgg ttccgctctg cccctctaat tcgacacaac
    11101 gagattgcga acacaagcga acccgcagtc ctgaaggtgt cgggccgata agctgataac
    11161 gccacgctac cagcgtcatc aatcctgaag caggttgtcg cgtagaaaga ggcgcagacg
    11221 acatcgactc accgcgatga tttagcgtaa ttcctacact acttgcctgc cacatcccct
    11281 ccccggcggc ttgcaccagc agcggaaaca ataatattgc taataaggtt ctcattattt
    11341 gccgccaatt gtcgccgtca tgcggatatg tcggttatca gacagttcca gattcgacag
    11401 gaccactaac tgcggcaagc tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg
    11461 gttcaccaac aatactggcg gcgcgcccag catctcctga cgggatagcg cttcctgagt
    11521 ttgcgccagt aaacgatccg ccagccctgg ctccagtcct cccccgccct gcagcgcctg
    11581 tagtaacaaa cgttccagcg gtgtatcgag gccaataaca tggacttcat ctttgccagg
    11641 aaaccactgc tgggtaatcg cccgtcccaa cgccacgcgc acgacggcgg ttaattcatg
    11701 tggatcgctt tggatgggcg catgttccgc cagcgtttcg agaatggtgc gcatatcgcg
    11761 aatcggtact ttttcatcga ggagattttg cagcacttta tgcagtgtgg tgagcgtgac
    11821 gacgccagga acgagatctt ccgtcagctt tggcatctcc tgggcgacgc gatccaacag
    11881 ctgttgcgcc tcctgacgac caaacagctc tgcggcatgc tggctaatga ggtggttaag
    11941 atgcgttgct accaccgtgc tggcctcaac cactgtgtac ccctgaatct gcgcctgttc
    12001 ttttagcgca ctttcaatcc agatagcatt caggccaaat gccggatcga cggtcgcctc
    12061 accaggtaac gtcccggcag cggttccagg gttaatcgcc agccagcgcc ccggataagc
    12121 atcaccactg ccaatctcca cgcctttcat caaaatgcga tagcgggcag gttgcagatc
    12181 catattgtcg cgaatgtgca ccactggcgg cagaaatccc atctcctggg caaatttctt
    12241 gcggatactg cgtatacggc ccaacaactc accatcctgc tggaaatcga ccatcgggat
    12301 cagtcgataa cccacttcca ttcccagaga atcttccagt tgtacatcgt tccacgtcgc
    12361 ttcgacaacg gtattattct ctgccatttt taccggtttg ggttcggcag gcgctttttg
    12421 ttcgcgtccg cgtatccacc aggccagccc gagcaatccg gcagtgaaca gcaaaaatac
    12481 caggttcggc attccaggca ccaggccgag taaaccgagc acggcggcgc ttaacaacat
    12541 aacgcttggg ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct
    12601 gacacgcgta acgatgaccc ccgcggcggt agaaatcacc agcgccggaa tttgtgccac
    12661 cagaccgtcg ccaatggtca atagcgtata actttccgcc gcgtgtccca tgctcatgcc
    12721 atgttgcagc acgccgacca gcaacccgcc gacaacgtta atgaccatga tgaggatccc
    12781 ggcgatggca tcgccgcgaa caaacttact tgccccgtcc attgagccgt aaaaatcggc
    12841 ttcctgagtc acttcggagc ggcgtttttt cgcctcatct tcaccaatca atccagcgtt
    12901 aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcacc
    12961 cacttctgcg atacgcccgg cacctttggt aatgaccata aagttgatga tcacgagaat
    13021 gacaaacacc acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc
    13081 gaccaccttc cctgccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga
    13141 agccacgtta agtgccagac gcaacaacgt agtaaacaac agaatggtcg gaaacgcagc
    13201 aaactcaagc gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaggcaat
    13261 attgaaggta aacaacaggt cgagtatgaa tgcgggcagt ggcagcacca tcatcgacaa
    13321 gatcaacagg atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc
    13381 gggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca
    13441 gggcttccgg caccggaaga tgagtaggtt gtacagggcg ctgtccacca gccagacgcc
    13501 agcgtttcag ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg
    13561 ggatttgttg accaatctcc gcatgtcgat acagcgctcg cgccagcggc ggcgcttcaa
    13621 gcgtcgggac gttattttca gcgccaattt cacgaatgcg cagcgcgacc agccctgcac
    13681 ctttagcgac cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat
    13741 agtgggtcgg gttattgaca atgacatcag ctttcggcac atcggccatc atccgacgcc
    13801 gtgcagcagc tcgctgcatc tgacggatcc gccctttaac atgagggtca ccttcgcttt
    13861 gtttgaactc atcacgaata tcctgccgcg acatacgcag ctttttcagg tggctgaaga
    13921 tttggaaaaa gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagtgcgc
    13981 atagcccaac cagatccatc gcattaccca tggcggtagt cggagactcg gccattaagc
    14041 gcatcatctg cggccagtga tgccagagat aaaaccccgt cacgctgcca accaggatgg
    14101 ttttcaaaat tgccttaagc aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc
    14161 ccggtagcgg gttgagtttg gaaaacttcg gctgcaagga tttgccgcta aataccagcc
    14221 cccccagcat gaccggagaa atgagcgcca ccagcaccac gccgctaatc agcggcagca
    14281 gcgccagcat ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtca
//
LOCUS       DAZPBM010000104        14297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPBM010000104 DAZPBM010000000
VERSION     DAZPBM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.104"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="ACHUZQ_002808"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="ACHUZQ_002808"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1026456.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHUZQ_002809"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHUZQ_002809"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1026457.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHUZQ_002810"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHUZQ_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR1026458.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="ACHUZQ_002811"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="ACHUZQ_002811"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1026459.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="ACHUZQ_002812"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="ACHUZQ_002812"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1026460.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHUZQ_002813"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHUZQ_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1026461.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHUZQ_002814"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHUZQ_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1026462.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHUZQ_002815"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHUZQ_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1026463.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="ACHUZQ_002816"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="ACHUZQ_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1026464.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="ACHUZQ_002817"
     CDS             complement(13978..>14297)
                     /locus_tag="ACHUZQ_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1026465.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAZPBM010000105        14217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPBM010000105 DAZPBM010000000
VERSION     DAZPBM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.105"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..183)
                     /locus_tag="ACHUZQ_002818"
     CDS             complement(<1..183)
                     /locus_tag="ACHUZQ_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026466.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
     gene            complement(225..762)
                     /locus_tag="ACHUZQ_002819"
                     /pseudo
     CDS             complement(225..762)
                     /locus_tag="ACHUZQ_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(1123..1224)
                     /locus_tag="ACHUZQ_002820"
                     /pseudo
     CDS             complement(1123..1224)
                     /locus_tag="ACHUZQ_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1409..1618)
                     /locus_tag="ACHUZQ_002821"
                     /pseudo
     CDS             complement(1409..1618)
                     /locus_tag="ACHUZQ_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
     gene            complement(1678..3126)
                     /gene="uxaB"
                     /locus_tag="ACHUZQ_002822"
     CDS             complement(1678..3126)
                     /gene="uxaB"
                     /locus_tag="ACHUZQ_002822"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1026467.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3353..4303)
                     /locus_tag="ACHUZQ_002823"
     CDS             complement(3353..4303)
                     /locus_tag="ACHUZQ_002823"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1026468.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4442..4801)
                     /locus_tag="ACHUZQ_002824"
     CDS             complement(4442..4801)
                     /locus_tag="ACHUZQ_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1026469.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(4801..5727)
                     /gene="glsB"
                     /locus_tag="ACHUZQ_002825"
     CDS             complement(4801..5727)
                     /gene="glsB"
                     /locus_tag="ACHUZQ_002825"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1026470.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(5791..7179)
                     /gene="sad"
                     /locus_tag="ACHUZQ_002826"
     CDS             complement(5791..7179)
                     /gene="sad"
                     /locus_tag="ACHUZQ_002826"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR1026471.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7280..8161
                     /locus_tag="ACHUZQ_002827"
     CDS             7280..8161
                     /locus_tag="ACHUZQ_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1026472.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8238..9353
                     /gene="yneK"
                     /locus_tag="ACHUZQ_002828"
     CDS             8238..9353
                     /gene="yneK"
                     /locus_tag="ACHUZQ_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1026473.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            9503..10693
                     /gene="ydeA"
                     /locus_tag="ACHUZQ_002829"
     CDS             9503..10693
                     /gene="ydeA"
                     /locus_tag="ACHUZQ_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1026474.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(10718..11383)
                     /gene="marC"
                     /locus_tag="ACHUZQ_002830"
     CDS             complement(10718..11383)
                     /gene="marC"
                     /locus_tag="ACHUZQ_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1026475.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            11595..12029
                     /gene="marR"
                     /locus_tag="ACHUZQ_002831"
     CDS             11595..12029
                     /gene="marR"
                     /locus_tag="ACHUZQ_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1026476.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            12049..12432
                     /gene="marA"
                     /locus_tag="ACHUZQ_002832"
     CDS             12049..12432
                     /gene="marA"
                     /locus_tag="ACHUZQ_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1026477.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            12464..12682
                     /gene="marB"
                     /locus_tag="ACHUZQ_002833"
     CDS             12464..12682
                     /gene="marB"
                     /locus_tag="ACHUZQ_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1026478.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(12739..14178)
                     /locus_tag="ACHUZQ_002834"
     CDS             complement(12739..14178)
                     /locus_tag="ACHUZQ_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1026479.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     SRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3748 a         3593 c         3204 g         3672 t
ORIGIN      
        1 ggcggataaa tcgacagatt cagcaagatc attgcttaaa aaccgtggat taaaataata
       61 ctctgcgtca acgggaaata ccgcagttgc taagccaagg gcaatggtaa gccgtgcaaa
      121 gcgaggcagt attaacacct gatgcatacg atatgcctta aatgcggcac gaaaggctgt
      181 cattattaat tccgattgag tttattatta attaggtcac tggcttactg catggttgcc
      241 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atccccggtt
      301 gcagcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      361 ttgctatttc ctgctttcat atttgttacg gtaatgaaat agggcgttgg attaataaga
      421 gtgagtttgc taccgctacg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      481 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      541 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      601 ctttcacggt cgcctggtaa ctgatgatta gtagcgttga ttatccgcag cgtattccag
      661 tgcgacagct gcgttggcaa cggtgctatg gcaagcaaag agcaataaaa gtaacactgt
      721 cgccataaag gaaatagagt agggggtacg cgtagtttgc atctgatgca ttctccatgt
      781 gttaagagga taagcgggaa aaccgttaca tcagacaatc gattaatgtt gctaatacat
      841 tggcgagtcg ccgggtttcc agacttaatg cgtttgtaat aaatgttggt tctcttttaa
      901 taggaaaacc ttgctacaaa gtaaatattt taaataacag taagtttaac tactgttgtt
      961 tattgatagt ttagatatta tgtgattttt catagcgctc caaatacaaa ccactcctca
     1021 ttaataatga gattcacatt ggtgaattgc tcgggtgtgc gtagccatgc cacacacacc
     1081 ctgtttcaat ttcagatgac gtatagccac cttaagattg aattatagat attgaacttt
     1141 gaaggtcgca tcagcgtttg cagtaccagc tacggactgc ccgagagcaa tataacgagc
     1201 ctggaatggc agtatattcg tgccacgata acatctttaa atcacaagga cacctaatct
     1261 gaacgaataa cgactaccag aaaagcactt cgcatcactc agacatgatt taacataata
     1321 tacattatgc gcacaaacat aaaccaaggg agaatccagt cgttgggccg gtcatgattg
     1381 ctcattaaca acgaccaaac cccatatctc acttactacc gtattctcgg cttaaccgtc
     1441 catgcaacct caacacattg gtttcaactg ccgtcaccac gttctccgga aaatctgtcg
     1501 gtaatgaagt cttcacgtta tccagtgctg ccggaatcat tctggcaaag tcacttagga
     1561 tttcatgcat ctgcacttcc gggaatctca gcacctttgc tgtcgccaaa aaatgttgta
     1621 actaatatag tgaaataaga aacccggtca aaaccgggtt cagaagtaac ggtgttatta
     1681 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
     1741 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
     1801 caacacaatc gctaccagtt cctgggtacc gataacatgg tcacgatgct ggctccagag
     1861 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
     1921 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
     1981 gccgtttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
     2041 accgttgagc gcgatagaca gcagctgatg cttatacggg ttacggaaac gcccggtgac
     2101 tgcactggcg aaagattcca gttcatcacg aggcaaatcc agtaccggaa taatttcttc
     2161 gtagatggct ttttcaacga atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt
     2221 atccagccct gcctggaacg ccaccggcac cagcgcggtg tgcgcaccgt tgaggattgc
     2281 cactttgcgt tctttatacg gtttaatatc gtcaacaatc agcacgttga gcggatattt
     2341 gtccaggcgc agctcagtcg ctaaggattt cggcccctga atcacaaaca ggtaaaagtg
     2401 ttcagcggtg tcgaggaaac catcgtgata acccagttct tcttccagtt tagccacttc
     2461 atcgcgcgga taaccggtaa cgatacggtc caccagcgta gaacagaagc tgttagcctg
     2521 atccagccat tgaataaatg cttctggcag tgcccactct tgcgcatagc gcagcaccag
     2581 ttcacgcaac gcgtcgccat tgtagtcgat caactcacac gggatgatga tccaaccttt
     2641 atccagcgca ccgttgaaat ggctaaagcg ttcgaacagc agacgggtca gttttgccgg
     2701 atagcttatc gctggcgcgt catcgaattt atcgcccgca tggtagctga tccccgcttc
     2761 ggtggtgtta gagaaaacaa agcgcatttc cgggttgtgc gccagtttca ggaattcatc
     2821 gtattcactg tagacgctga tttcacgatt aaccgagcga atcagacgcg cgtcgctgac
     2881 cgcttccccc ttctcattca ggccacggat aatggtggta tacagaccat cctgcgtgct
     2941 cagtgacggc gggaatgaag tttcaatcgg acgaacaacg accacgccag aattcagatc
     3001 ggtgtgctca ttcaggagat cgatttgcca gtcaacaaag gcgcgcagga agttaccttc
     3061 accaaactga atgatacgtt ctggatactg tgcaccggga aaatcgcgac gatttagtgt
     3121 tttcacaatg ggttcccttc tgattagtca tacaacctgt ttgaattggt acgacaggtt
     3181 agcaaacttt aatacgccga acccctgttt tgatcaactc ctgatgatta atgagcagtt
     3241 ttatgagaaa agtgtggcgc ggatcatggt ttaatcgagg aaatacgcct tttcctggat
     3301 cataaagtgg tagaacacat tgcattcaaa tcgcgcggaa tgaataaaga tgtcagacaa
     3361 cttcctcacc gtaacgcata gtgctggtac ggttgcgccc atctttcttc gagcgataca
     3421 gacacgtatc ggcttcgacc atcaatttat taaaatcatc ggtcagcgtt cggtacgatg
     3481 cgcgaccact accgacgcca atacttaccg tgagataaag cgttttttgt tgccaggtga
     3541 atggttgcag ttcaacgcct ttacggattt tttccgccat tagcagacca tcgacaggat
     3601 tcaccgacgg cactgcaaca gcaaattctt cgccgcccat gcgcgccacc agccccttat
     3661 caccgacaat cttctgaata tgccgggcaa acacgcttaa cactttatcg ccacattcat
     3721 gtccatagtt atcgttaatg cttttgaaat agtcgatatc aagcagcatg accgtcagag
     3781 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
     3841 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
     3901 caatcagaca gtaattaaat agccgtaagg cacttactgg ctcatactta tttttcccca
     3961 gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
     4021 tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
     4081 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacccccccg
     4141 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
     4201 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
     4261 cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
     4321 aattgcgggt ggcaattatc tcaacgtaat aagtaaattc tcataaagga tagcatgttc
     4381 gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt
     4441 ctcatggctg attcatctgt acaaccagcc agtgatagat taccgccacg atataacgtt
     4501 gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaaccgc
     4561 gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
     4621 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
     4681 gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc atgcccagac
     4741 gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agagactgca
     4801 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag
     4861 cgagttacct gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
     4921 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
     4981 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgcgcctg
     5041 cattggcgtc actacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
     5101 aaacgtccga gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg
     5161 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
     5221 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt atgaaatatc
     5281 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttaatcg
     5341 cccttgcaac atatcgcaga ccaccagcgc accagcatta ataaacggat tacgcggaat
     5401 accctgttcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
     5461 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
     5521 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
     5581 gtcaacggta cagatagcaa tgcccaatcg ggaaccgtct actgtagcca gcgccggaat
     5641 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
     5701 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
     5761 tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca ccgtctggat
     5821 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
     5881 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
     5941 ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga
     6001 aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaacggt
     6061 gattgccgca acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt
     6121 cgccagaacc gttggcggat agtagttacc tgccccagcc atcttttccc cgcccagtaa
     6181 caaacgcgca ccctgcgcca gggttttctc tacctgatga tgcagctcat cacgtaaatc
     6241 aaaacgagcc attggtccga gagtgttctc ttcgtcacgg ggatcgccca ttttcaaggc
     6301 tgccgcagct gccacaaaac gttcggtaaa tgcagaagca attccctctt cgataataaa
     6361 gcgttttgcc gctgcacata cctgtccggt attctgataa cgtccggcta ccgccgcttt
     6421 aaccgccagt tccagatcgg catcgttaag cacaataaac ggatccgaac cgcccagttc
     6481 cagtacgcat tttttcagtg ccgctccagc ctgtgcgcca atagccgctc cagcacgaac
     6541 acttccggtc accgtgacag cagcaatgcg cgagtcttta atcatctgac tgacaccgtc
     6601 gttgtcggca ttcagccagc catatacgcc ttgcgggatt cccgcatctt taaacacctg
     6661 ggcaatgagc tgagcacatc ccatcacatt cggcgcatgt ttaagtaagt agccgttacc
     6721 tgcaagaatg atgggaacag cgccacgcat cacctgccat aacggaaaat tccacggcat
     6781 aatcgccaga atcgtcccca acggtcgata ctcaataacc gcctgctgat tttccaccag
     6841 tgtaggttcc gccttcagca ttgccggacc atgttctgca taccagtcac acaaattcgc
     6901 cgatttcgcc acttcagcgc gcgcctggtt gattggtttg cccatttcgc gggtgatcat
     6961 ttgcgccatt tcttcgctac gggcgcgcag agccttaccg atatcacgca ttttttcagc
     7021 acgataatct atatttgtct cgcgccagtc gcgaaagcct gctgccgcca gctcaagtgc
     7081 gttttcgata tcgttagcgc cagcccacgg cagcacagaa agttgttcac ccgtggcagg
     7141 atttattgaa attgcatgag ttgccggagt aatggtcatc ggggtatctc ctttatgagt
     7201 catggtatga agatacgcag atttactctt gctttaaaat gaataatatt aagccactta
     7261 ttcacgaatc gagaatgtta tggatctgac ccaactggag atgttcaacg cggttgccga
     7321 agctggcagc attacccagg ctgcagcaaa agtgcatcgc gtaccgtcga atctcactac
     7381 ccgtctacgc cagctggaaa cagaactggg ggtagatctg tttattcgcg agaatcagcg
     7441 tttacgtctc tctcctgccg ggcataactt tttacgctat agccaacaaa ttctcgcgtt
     7501 agtggatgaa gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat tttctcttgg
     7561 ttcgctggaa agcaccgcag cagtgcgcat tccggccacg ctggcggaat tcaaccatcg
     7621 ttatcccaaa attcagtttt cactttccac cggcccttcc ggcactatgc tggaaggagt
     7681 gctggaggga aaactgaatg cggcatttat tgatgggccc attaaccata ctgccattga
     7741 cgggataccg gtataccgcg aggagctgat gatcgtcacg ccacaaggtc atgcgccagt
     7801 aatccgcgcc agtcaggtta acggcagtaa catttatgcc ttccgcacca attgttcgta
     7861 tcgtcgccac ttcgagagct ggtttcatgc tgacggtgcc gctccgggaa ctattcacga
     7921 gatggagtcg tatcacggga tgttggcctg tgtggtcgca ggagcaggca ttgcgcttat
     7981 tccacgctct atgctggaaa gtatgccggg gcatcaccag gttgaagcgt ggccgttagc
     8041 tgagcaatgg cgttggttaa caacctggct ggtctggcgt cgcggtgcga aaacgcgtcc
     8101 gcttgaggca tttattcaac tactggatgt gtctgactcg gcaaaacaga gttaccaatg
     8161 aactattttg atagttcttg cggctaatat gctctatata gtgatgttcc gatgacttat
     8221 gactatatgg ggcaaatatg gttacgccag taagcatcag caattacata tctcttcctg
     8281 atgattttcc cgttcgtaat attgcaccac aggtaaaaga agttttaaag gactttattg
     8341 atgcgctaag tacaataata tgtgatgaag agtggcgcac gtctttaaac atcaactcag
     8401 ccacaaaaaa gatatttaat aaccttgata acttatcata tattcagagg acttctttcc
     8461 ggggtaacga cacgctatac aatgaaaagg ttcagtttaa actcacttat cccgtcaaga
     8521 atggaagaca caaagaaaat attgaatttc aggtagtaat aaatttaagc cctatttatt
     8581 tagataattt tcgccatgat ggagaaatta atattttttg cgctcccaac ccaaagcctg
     8641 tcactatggg gcgcgtattt cagaccggcg tcgagcgtgt gctctttctt tttctgaatg
     8701 attttattga acaatttcca atgttcaacc ctggcgttcc gatcaaaata gcgcatacac
     8761 cacatattga acccctgcct ccggatcacc ataccgctgc agattactta cgccagtttg
     8821 atttgcttgt cctgaatttt atctctcgcg gtaattttgt catactcccc cgattatgga
     8881 ataactctga ggttcacaga tggttcgtca ataaagatcc taacttgatc accgccattc
     8941 tcgacataac ggacagcgaa ttaaaagagg atttattgca aagcctgatg gattcattag
     9001 gttctaacaa acatgtacta cccgaagtct gcatctgctt tttattcctt ttagcagaac
     9061 aagaatctcc tcattttcag gacttgtttt tatttttcgc caatatgtta ttgcactatc
     9121 accaatttat gaatcccaat gaaagtgatt tgaatgacgt gttgatgcca gcatcattaa
     9181 gtgatgataa aattatcaag catatggcgc gcaggaccct cgaactgttt gtaaaaaatg
     9241 aaacaccgcc aaaagttact cacgaagacc tggtgaaaaa caggcctcgc tcccctgtca
     9301 gaccacctat acccgcaacc tccaaaacgc cagacctccc tgaacgtcat taaaccgtga
     9361 tgttaccgac tctctgacgc ggaacagaat cagcgtcaga gaaacggaaa acgcgatcca
     9421 gatcacaaat acattgtatt cacatcatta accgttttaa gatcatttca tcactttttc
     9481 gcaactcacc cgataatctg ttatgacaac aaacactgtt tcccgcaaag tggcgtggct
     9541 acgggtcgtt acgctggcag tcgccgcctt catcttcaac accaccgaat ttgtccctgt
     9601 tggcctgctc tctgacattg cgcaaagttt tcacatgcaa accgctcagg tcggcatcat
     9661 gttgaccatt tacgcatggg tagtagcgct aatgtcattg ccttttatgt taatgaccag
     9721 tcaggttgaa cggcgcaaat tactgatctg cctgtttgtg gtgtttattg ccagccacgt
     9781 actgtcgttt ttatcgtgga gctttaccgt tctggtgatc agtcgcattg gtgtggcttt
     9841 tgcacatgcg attttctggt cgattacggc gtctctggcg atccgtatgg ctccggccgg
     9901 gaagcgggca caggccttga gtttaattgc caccggtaca gcactggcga tggtcttagg
     9961 tttacctctc gggcgcattg tggggcagta tttcggttgg cgaatgacct tcttcgcgat
    10021 tggtattggg gcgcttatca cccttttgtg cctgattaag ttacttccct tactgcccag
    10081 tgagcattcc ggttcattga aaagcctccc gctattgttc cgccgcccgg cattgatgag
    10141 catttatttg ttaactgtgg tggttgtcac cgcccattac acggcataca gctatatcga
    10201 gccttttgtg caaaacattg cgggattcag cgccaacttt gccacggcat tactgttatt
    10261 actcggtggt gcgggcatta ttggcagcgt gattttcggt aaactgggta atcagtatgc
    10321 gtctgcgttg gtgagtacgg cgattgcgct gttgctggtg tgtctggcac tgctgctacc
    10381 tgcggcgaac agtgaaatac acctcggggt gctgagtatt ttctggggga tcgcgatgat
    10441 gatcatcggg cttggtatgc aggttaaagt gctggcgctg gcaccagatg ctaccgacgt
    10501 cgcgatggcg ctattctccg gcatatttaa tattggaatc ggggcgggtg cgttggtagg
    10561 taatcaggtg agtttgcact ggtcaatgtc gatgattggt tatgtgggcg cggtgcctgc
    10621 ttttgccgcg ttaatatggt caatcattat atttcgccgc tggccagtga cactcgaaga
    10681 acagacgcaa tagttgaaag gcccattcgg gcctttttta atggtacgtt ttaatgattt
    10741 ccaggatgcc gttaataata aactgcacac ccatacatac cagcaggaat cccatcagac
    10801 gggagatcgc ttcaatgcca cccttgccca ccagccgcat aattgcgccg gagctgcgta
    10861 ggcttcccca caaaataacc gccaccagga aaaagatcag cggcggcgca accatcagta
    10921 cccaatcagc gaaggttgaa ctctgacgca ctgtggacgc cgagctaata atcatcgcta
    10981 tggttcccgg accagcggta cttggcattg ctagcggcac aaaggcaata ttggcactgg
    11041 gttcatcttc cagctcttcc gacttgcttt tcgcctccgg tgaatcaatc gctttctgtt
    11101 gcggaaagag catccgaaaa ccgataaacg cgacgattaa gccgcctgca attcgcagac
    11161 cgggaatcga aatgccaaat gtatccatca ccagttgccc ggcgtaatac gccaccatca
    11221 tgatggcaaa tacgtacacc gaggccatca acgactgacg attacgttcg gcactgttca
    11281 tgttgcctgc caggccaaga aatagcgcga cagttgttaa tgggttagct aacggcagca
    11341 acaccaccag ccccaggcca attgctttaa acaaatctaa cattggtggt tgttatcctg
    11401 tgtatctggg ttatcagcga aaagtataag gggtaaacaa ggataaagtg tcactcttta
    11461 gctaaccttg catcgcattg accaaaactt gaaccgattt agcaaaacgt ggcatcggtc
    11521 aattcattca tttgacttat acttgcctgg gcaatattat cctctgcaac taattacttg
    11581 ccagggcaac taatgtgaaa agtaccagcg atctgttcaa tgaaattatt ccattgggtc
    11641 gcttaatcca tatggttaat cagaagaaag atcgtctgct taacgagtat ctgtctccgc
    11701 tggatattac cgcggcacag tttaaggtgc tctgctctat ccgctgcgcg gcgtgtatta
    11761 ctccggttga actgaaaaaa gtgttgtcgg tcgacctggg agcactgacc cgtatgctgg
    11821 atcgcctggt ctgtaaaggc tgggttgaaa ggttgccgaa cccgaatgat aagcgcggcg
    11881 tactggtaaa acttaccacc agcggcgcgg caatatgcga acaatgccat caattagttg
    11941 gccaggacct gcatcaagaa ttaacaaaaa acctgacggc ggacgaagtt gcaacacttg
    12001 agcatttgct taagaaagtc ctgccgtaaa caaaaaagag gtatgacgat gtccagacgc
    12061 aatactgacg ctattaccat tcatagcatt ttggactgga tcgaggacaa cctggaatcg
    12121 ccactgtcac tggagaaagt gtcagagcgt tcgggttact ccaaatggca cctgcaacgg
    12181 atgtttaaaa aagaaaccgg tcattcatta ggccaataca tccgcagccg taagatgacg
    12241 gaaatcgcgc aaaagctgaa ggaaagtaac gagccgatac tctatctggc agaacgatat
    12301 ggcttcgagt cgcaacaaac tctgacccga accttcaaaa attactttga tgttccgccg
    12361 cataaatacc ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc attaaatcat
    12421 tacaatagct agttgataac gtgacaacgt cactgaggca atcatgaaac cactttcatc
    12481 cgcaatagca gctgcgctta ttctcttttc cgcgcagggc gttgcggaac aaaccacgca
    12541 gccagttgtt acttcttgtg ccaatgtcgt ggttgttccc ccatcgcagg aacaaccacc
    12601 gtttgattta aatcacatgg gtactggcag tgataagtcg gatgcgctcg gcgtgcccta
    12661 ttataatcaa cacgctatgt agttattgtc aggctctgat cacagagcct gacatctccg
    12721 taatatcttc ctcaagtctc aaatattttc gccattgttg gcgatgagat cccgatacca
    12781 gtaaaacgac gctttacgta tccgttccag cgttcccttc ccttcgttgt ctttatcgac
    12841 ataaatcatt ccgtagcgct ttttcatttc ccctgttccg gcagaaacca ggtcaatgca
    12901 gccccacggg gtgtagccaa ttaagtcaac accatcttca actacggctt ttttcatttc
    12961 ccgaatatgg gaagcaaagt aatcaatgcg atagtgatcg ttcaccgtgc cgtcagcttg
    13021 tctctggtca accgcaccaa atccattttc gacaataaac agcggcaact ggaaatgatc
    13081 ccagaaccag ttgagtgaat aacgtagccc tgccggatca atttgccatc cccattcgga
    13141 tgtatcgata taagggttgc tgaccaaatc tcgtgattca acataatcca gttgcgggtt
    13201 atcgggagaa aattgtgtcg taaaagacat gtagtagcta aaaccgataa agtcgacaca
    13261 tccactggca agaatcgcgt tatcttctgg tgtgatatcg aggttgaatc ctttcctggc
    13321 aaagtaattc agcatatgtt gcggataata tccacgagca tgaacatcag taaaccagta
    13381 acgacgatgc atcgctttcg tggccatcat catatcgttg ggtgcacacg tcagaggata
    13441 gatggggcac atggcgatca tacagccaat gttaaattca ggattgatcg attttccagt
    13501 ctgtaccgcc agggcactgg caactaactc gtaatgtacc gcctggtaca ttatttgttc
    13561 gcgctcattg atatcttcct ctggcgaata caagataccg gaattagtaa atggacacag
    13621 gctttcgctg aaattcacct gattattgat ttcgttaaac gtcatccagt actttacttt
    13681 ttctttatag cgcgtgaaga ccgttgatgc gaagcggata aaaaagtcga tcagcttacg
    13741 gtttcgccag ccaccatatt ttgtcaccag atgataaggc atctcaaaat gcgaaagcgt
    13801 caccacaggt tccattcctt gcttcaggca ttcatcgaac agatcatcat aaaattgtaa
    13861 accctcttca ttcggctcct gttcgtcacc ttgcggaaag attcgtgtcc aggcaatgga
    13921 agttcgaaag catttgaatc ccatctcggc aaataactga atatctgttt tatagcgatg
    13981 ataaaaatca attgcttcat gattgggata attaagcccg tcgataacgc cttctgtcac
    14041 ttcacgcggc accccgtgag cgccagcagt catcacatca gcgatactga tgccttttcc
    14101 tccttcattc cagccacctt ccaactgatg cgcggctacc gcgccacccc ataaaaaacc
    14161 ttttttaaat cctgacatac acaactcctt aactaaaata gattaccacc agacttc
//
LOCUS       DAZPBM010000106        14005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPBM010000106 DAZPBM010000000
VERSION     DAZPBM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.106"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..262)
                     /locus_tag="ACHUZQ_002835"
     CDS             complement(<1..262)
                     /locus_tag="ACHUZQ_002835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026480.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAVSMYGLYIDPQ"
     gene            297..1022
                     /locus_tag="ACHUZQ_002836"
     CDS             297..1022
                     /locus_tag="ACHUZQ_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1026481.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            1515..2261
                     /gene="mipA"
                     /locus_tag="ACHUZQ_002837"
     CDS             1515..2261
                     /gene="mipA"
                     /locus_tag="ACHUZQ_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1026482.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2351..3205
                     /gene="yeaE"
                     /locus_tag="ACHUZQ_002838"
     CDS             2351..3205
                     /gene="yeaE"
                     /locus_tag="ACHUZQ_002838"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR1026483.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(3256..4140)
                     /locus_tag="ACHUZQ_002839"
     CDS             complement(3256..4140)
                     /locus_tag="ACHUZQ_002839"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1026484.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4224..5219)
                     /gene="gapA"
                     /locus_tag="ACHUZQ_002840"
     CDS             complement(4224..5219)
                     /gene="gapA"
                     /locus_tag="ACHUZQ_002840"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1026485.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5561..5974
                     /gene="msrB"
                     /locus_tag="ACHUZQ_002841"
     CDS             5561..5974
                     /gene="msrB"
                     /locus_tag="ACHUZQ_002841"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1026486.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6016..6288
                     /locus_tag="ACHUZQ_002842"
     CDS             6016..6288
                     /locus_tag="ACHUZQ_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1026487.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(6449..7090)
                     /gene="pncA"
                     /locus_tag="ACHUZQ_002843"
     CDS             complement(6449..7090)
                     /gene="pncA"
                     /locus_tag="ACHUZQ_002843"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1026488.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7101..8117)
                     /gene="ansA"
                     /locus_tag="ACHUZQ_002844"
     CDS             complement(7101..8117)
                     /gene="ansA"
                     /locus_tag="ACHUZQ_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1026489.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(8284..10140)
                     /gene="sppA"
                     /locus_tag="ACHUZQ_002845"
     CDS             complement(8284..10140)
                     /gene="sppA"
                     /locus_tag="ACHUZQ_002845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1026490.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            10301..10852
                     /locus_tag="ACHUZQ_002846"
     CDS             10301..10852
                     /locus_tag="ACHUZQ_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1026491.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10969..12012
                     /gene="selD"
                     /locus_tag="ACHUZQ_002847"
     CDS             10969..12012
                     /gene="selD"
                     /locus_tag="ACHUZQ_002847"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1026492.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGHIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            12017..13978
                     /gene="topB"
                     /locus_tag="ACHUZQ_002848"
     CDS             12017..13978
                     /gene="topB"
                     /locus_tag="ACHUZQ_002848"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1026493.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3595 a         3374 c         3639 g         3397 t
ORIGIN      
        1 gttgggggtc tatatataat ccatacatag aaacagcgga gatagcaata ccgaaaacca
       61 tccacatatc tatagggctt ttatttgtcg atttgaccgt agtgtacttt ttgactaagt
      121 ccgtacctga tagcactgtt aaaattaatg ataatatagc gagggcagta tttacattaa
      181 aatttagctt agcgaataaa agcaacatta gtaaaataaa aataagtaac gctatgatta
      241 aaaatattcc tttcttgtcc ataaataccc cgtcgtattt ttaaaaaagg tatcttatgg
      301 caaggataag taaaaaggaa tctgctaatc acaataaagt gatggaatta atacattcag
      361 ataagctcct aacacaagaa gatagggaat tcatattcac caattatagg ggggatggga
      421 taggggctac aggtgccttc ttcacacctg aaattttagc atgggatttt gtacttgatg
      481 ctgactgtac tggtgactgt attgaactat gtgctggtat cggtcgcctc agctactacc
      541 agtacttaag gaataaacca acgcatatta catgcgtgga actcaatcct gagtacgtaa
      601 tgatcgggaa gagggtacta ccagaggcag aatggatcac tggtaacgca ctacagtact
      661 caccagaccg tttctatgat gttgcctatg gtaatccacc ctttgggaag atcagtacct
      721 caaaagcgta tacaggccgt tataagggta gtgaatttga atacaaggtg attgaacatg
      781 caagtacctt tgcgtcctat ggtgcatgga tagtaccgca aggttccgca ggctttgtct
      841 acagtggggt acattgttat gagcgtagag aatcatgtaa gtaccagaag tttgtgaagg
      901 atactggcta tgagctacat ccaggtgtag gcatcgatac aagtatctac agggatgaat
      961 ggaatggtac taaggtgata tgcgaatcgg tgttagtaga tgatgacggt acaaatctgt
     1021 aactaaatta gttaagaaat cataaagata gtagtacttg caaatgttcc cattgatgaa
     1081 gttatgcgtc ctttcgtttt ttagcacagg ttaagagccg ttcatatgaa tagtagaggc
     1141 tcccggaaga ggtaaactga acgacgtctc ttgctaaaac acagcaactc tcatgccatt
     1201 ttatcgacga tgagaacaac gtgcccggtg atacaccttc aaaattaagc gtagatggca
     1261 aatggaaaac ttgcctgcac ggctttctta atttcaatgg catatcaaca actcatccat
     1321 aaattttgca taattaatgt aaagacaagg ctcgccagta acgctaaatt catttggctg
     1381 taagcgcggt gtcatccgcg tcaggaaaat taaacagtta ctttaaaaaa tgaaaacgta
     1441 aaaaggttgg gtttcgatgt attgacgggt aaactttgtc gcccgctaaa catttgttta
     1501 aggaatgatt aattgtgacc aaactcaaac ttctggcact tggagtgctt atcgcaacgt
     1561 ctgcaggcgt agcgcacgct gaaggtaaat tttccctggg cgcaggcgtc ggtgtcgttg
     1621 aacacccata taaagattac gataccgatg tttacccagt accggtaatc aactatgaag
     1681 gcgataactt ctggttccgt ggcttaggtg gtggttacta cctgtggaat gacgcaacgg
     1741 acaaactttc aattaccgct tattggtcgc cgctttactt caaagcgaaa gacagtggcg
     1801 atcaccaaat gcgtcacctg gatgaccgta agagcaccat gatggctggt ctgtcttatg
     1861 ctcactttac ccagtacggt tacctgcgta ccaccctggc tggcgatacc ctggataaca
     1921 gcaacggcat tgtctgggat atggcctggt tgtatcgtta caccaacggc ggcctgacag
     1981 tgaccccggg tatcggtgtg cagtggaaca gcgaaaacca gaacgaatac tattatggcg
     2041 tatcgcgcaa agagtccgct cgcagcggtc tgcgtggcta taacccgaac gacagctgga
     2101 gcccttacct ggagctgagc gccagctaca acttcctcgg cgactggagt gtttacggta
     2161 ccgcgcgcta tacccgtctg tctgatgaag ttactgacag cccgatggtg gataaatcct
     2221 ggactggcct gatttctacc gggatcacct acaaattctg ataatgcatc tttccagggc
     2281 attttgtgca taaaaacagg gcgtcacgcc ctgttttgca ttacggtggt gcaatacctg
     2341 ggaggaaaca atgcaacaaa aaatgattca atttagtggc gatgtctcac tgccagccat
     2401 agggcaggga acatggtata tgggcgaaga tgccagtcag cgcaaaacag aagttgctgc
     2461 actacgcgcg ggcattgagc tcggtttaac cctcattgat accgccgaaa tgtatgccga
     2521 tggcggtgcc gaaaaggtgg ttggggaagc attaaccggt ctgcgtgata acgtctttct
     2581 cgtctctaaa gtctatccgt ggaatgctgg cgggcaaaaa gcgataaatg catgcgaagc
     2641 cagtttacgc cgtctcaata ctgattatct cgatctctac ttattacaca ggtctggcag
     2701 tttcgctttt gaagagactg ttgcagcgat ggaaaaattg atcgcccagg gaaaaatccg
     2761 ccgctggggc gtttctaacc ttgattatgc tgatatgcag gaactctggc agctgccggg
     2821 gggaaatcag tgtgccacta atcaggtgct ttaccatctc ggttcacgag ggattgagta
     2881 cgatctactc ccctggtgcc agcaacagca gatgccggtg atggcttaca gtccattagc
     2941 ccaggccggg cggttgcgca atggactgtt aaaaaacgcg gtagtcaacg aaattgcaca
     3001 tgctcacaat atcagctcgg cacaagtatt gttggcgtgg gtaatcagcc atcagggtgt
     3061 gatggcgatt ccaaaagcgg ccacgattgc ccatgttcaa cagaatgcgg ctgtgcttga
     3121 ggtcgaactt tcgtcagcgg aattaaccat gctggataaa gcgtatccgg caccaaaagg
     3181 aaaaactgcg ctggatatgg tgtgatgttg atacgtgccg gatgcatcgc acccggcaca
     3241 caacattaac gcaaattaac gtttcgcaac gcgaatggat tgcgccagat gtgcaggttt
     3301 ctctttggtg actttttgcg tttctgaagc gtaagccgtt tctacacaaa caaatgtttt
     3361 gtagccatca tccggcatat cgcccatgct aattgaaagc gccggtcccg ggttccagcc
     3421 tacaacgttc agatgatgct ggtggcctac ggcgataata cgattcagcg cttcatcatt
     3481 aatcacgctg caatcttgcg gattcagata cacgcgatcg gtacggtcag ggaaggtctg
     3541 aataccgtcg gtcagtacat cttctttcgc gtcattcact ttatcaatga agcgatcgcc
     3601 cagcccactg acgcttacct tagcgatatc acccacgtta aagtaggtat gcagggcaga
     3661 ggtggtttca aattcgccat gtgattcaag atcgatttca caggttttac ccacgcggaa
     3721 atgcgctaac agcgtaaagt cgtgcggaca gaattttttc gtctcttcgc tttgcgtcaa
     3781 ttcaaaagtc agcgctacgc catcagcatc ttcattatgt gatttcagcg tccacggcag
     3841 gttgcgggca aaaccgtgcg caggcagacc ttgttgtgcc gccggaccaa accacggcca
     3901 gcaaaccggt acgccaccgc gaatagcgac gccatttttg aacggtgtgt tgttgctcaa
     3961 ccacagaact tcttcttcac ccgcaggttt ccacgagaga aggtgtgcgc cctgtaatgc
     4021 aaaagaggct tttacttggg gatgatcgac cacaatgagg tccagttcat ccagtttacg
     4081 acgggagagg acaggggaga tttgttcgat gaccggaagg gcaaaaattt tcttaatcat
     4141 gacgcagtcc tttaacttca ttttatcagg taaaaaaaag agcgaccgaa gtcgctcttt
     4201 ttagatcaca gtttcatctc aacttatttg gagatgtgag cgatcaggtc cagaactttg
     4261 ttggagtaac cggtttcgtt gtcgtaccag gataccagtt tcacgaagtt gtcgttcaga
     4321 gcgataccag ctttagcatc gaacacggaa gtgcaaactt cgccgttgaa atcggtagat
     4381 actacgtcat cttcggtgta gcccagaacg cctttcattt cgccttcagc agcggcttta
     4441 acggcagctt tgatctgctc gtaagttgca gctttttcca gacgaacggt caggtcaact
     4501 acggatacgt tcggggtcgg aacgcggaac gccataccag tcagtttgcc attcagttct
     4561 ggcagtactt tacctacagc tttagcagca ccggtagagg acgggatgat gttctgggaa
     4621 gcgccgcggc cgccgcgcca gtctttgtga gacgggccat caacggtttt ctgagtagcg
     4681 gtagtagcgt gaacggtggt catcagacct tcgatgatgc cgaagttatc gttgataact
     4741 ttagccagcg gagccaggca gttggtggtg caggaagcgt tggaaacgat gtcttggcca
     4801 gcatatttgt cgaagttagc gcctttaacg aacatcggag tgttgtcttt agacggacca
     4861 gtcataacca ctttcttcgc accagcggtg atgtgtttac gagcagtttc gtcagtcagg
     4921 aacagaccag ttgcttcagc gacaacgtca acaccaactt cgtcccattt caggttagcc
     4981 ggatcacgtt cagcggtaac acggattttt ttaccgttaa cgatcagatg accgtctttc
     5041 acttcaacgg taccgtcgaa acggccgtga gtggagtcat atttcagcat gtatgccatg
     5101 taatcagcgt ctaacaggtc gttgattgca acgatctcga tgtcagaacg tttctgagca
     5161 gcacggaaaa caatgcgacc gatacggcca aaaccgttga tacctacttt gatagtcata
     5221 tattccacca gctatttgtt agtgaataaa aggttgcctg taaaattaca aaaaccttac
     5281 gcagcgtcaa gcggaatcgt gtcaatcatt gcgacaaatc aatcctgtgc ctaagcatta
     5341 cgcgactgac tcgcctcact cttcctttgg gcttgaaacc acatggggtc ggcgccccga
     5401 attttaaagg gcaattacga taaaaatgtg attttcatca cgatttaggt gcagccattt
     5461 cgtcgagatc aagtttagga caaaattttg ccagttggtg ttaatgtttt gttagaatca
     5521 gtcacgtaat gtgagcacga ttaaagtgag atgtgagcaa atggctaata aaccttcggc
     5581 agaagaactg aaaaaaaatt tgtccgagat gcagttttac gtgacgcaga atcatgggac
     5641 agagccgcca tttacgggtc gtttactgca taacaagcgt gacggcgtat atcactgttt
     5701 gatctgcgat gccccgctgt ttcattccca aaccaagtat gattccggct gtggttggcc
     5761 cagtttctac gaaccggtga gtgaagaatc cattcgttat atcaaagact tgtcacatgg
     5821 aatgcagcgc atagaaattc gttgcggtaa ctgtgatgcc catctggggc atgtcttccc
     5881 tgacgggccg cagccaacgg gcgaacgtta ttgtgttaac tctgcttctt tacgcttcac
     5941 tgatggcgaa aacggcgaag aaatcaacgg ttgaaaaaac gattcagcaa cttattccac
     6001 aggagcggaa aaattatgaa tcttgatgac attatcaaca gcatgacgcc tgaggtatac
     6061 cagcgtttgt cgaccgccgt tgaactgggg aaatggcccg atggcgttgc gttgaccgag
     6121 gaacaaaaag cgaactgcct gcaactggtg atgctgtggc aagctcgcca taataccgaa
     6181 gcacagcata tgacaattga cacaaatggt cagatggtca tgaagagcaa acagcagtta
     6241 aaagaagatt ttggtatctc agcaaagcct attgctatgt ttaagtgaat gagttatcta
     6301 gtatttaagc ctgttattaa gggagggcaa atatacttgt taggacgtcg tgttttagct
     6361 gcaagtaggt taaatgcaac actgacaagt attatctggc ctacaatgtc attgaggaaa
     6421 tgtaggccag agatgaattc aactaatatt acccctgtgt ctcttcccag tctgccagcg
     6481 tatatagcgt tgccccagct gctgacatct ccataaacgc gtgcgcactg tcctggggct
     6541 ggatattcac gccacgacaa ccatcggtaa tcacatttac cttataacct aactgtaacg
     6601 cgtccagcac ggtaaacttc acgcaatagt cagtagccag gcccataacg atcaattcat
     6661 cgatttcatg atcgcgtaac cagtcatcga gcgaggtttt ctgccgacgg ccgttatcaa
     6721 aaaaggcact gtaactgtca actaaaggat tttcgccttt atggaacacc gctgcgatcg
     6781 ctttttggtt cagtaacgga tgtaattgtg cgccttcact gttctgcaca cagtgatctg
     6841 gccagaaggt ttgtggcaaa ccgtcgagtt ggcctggcgt ataaggctct acaccgtgct
     6901 gactggcaaa actgccgtga ttcgccgggt gccagtcctg actggcgata accgcttcac
     6961 cgcgcgactg gcaccagtca atcaggcggt tagcgacatc caccgtactg tcaccttccg
     7021 gcacggcgag cgcgccacca gcacagaaat cattttgtaa atcgaccagt aacagggcgc
     7081 gagggggcat tacagtctcc ttaatcatcc ggcgtcagtt cgccgcgcag gttttggctc
     7141 atggccttgc gaatggtttc agtatccagt tcctggctca gcaggtaatg cagtttggtt
     7201 agcgtggctt ctacagtcat atctgcaccg ccaataacgc cggcatgggc gagggcgtta
     7261 ccggtggcat aaccacccat attcacttta ccggacatac attgtgtcag gttgaccacc
     7321 acaataccgc gatcgctggc ttcttgtaat tcctgcagga aggctttgtt ttgtggcgca
     7381 ttacccacgc cataggagcg cagaatcaat gctttcaccg gttggcgcag aaaattgcgc
     7441 acgacgtcag cagaaatccc tggataaatc gtcactacgc caattggttg tggcgtgatt
     7501 ggatgaacga ttaattcccc ttcaccgtgc ggggcgggtg gcgtattcaa acgacgaata
     7561 tggataccag cttccagtaa cggaggaagg tttggagagg caaacgcatc aaaaccatcg
     7621 gcatgggctt tggtagtgcg gttgccgcga tacaatcggt tattgaaaaa gagcgttact
     7681 tcgttgatcg gataattcgc cgcaacgtac aacgcattca gcagattaat ttgtccgtca
     7741 gagcgtaact cagccagcgg gatttgtgac cctgtcacaa tgaccggttt accgagattc
     7801 tcgagcatga acgacagcgc agaggcagta tacgccatcg tgtcggtgcc gtgcaggatg
     7861 acaaaaccat catagtcgtc atagtgcgct ttaatatctt cagcaatatg ctgccagtct
     7921 tctggcgtca tatcagaaga atccatcagc ggcgtatatt catgaatggt gaaatctggc
     7981 atctccgggc gatggaattc cggcatcagc gccagttggc gttgtagatg acctgacacc
     8041 ggaatataac cctgctcgga acgctgcatc ccgatggtcc cgcccgtgta ggcgacgtaa
     8101 attgatttct tttgcatgat attgatatga atgatatcga aagagcaaaa gtatatacgt
     8161 gaggcaggag gagcgcacct gatactatgc ggataaggaa atatggggcg agacagcggg
     8221 aagaggattc gtcgacgtaa aaaataatgt ggccgacgat ctgtcggcca ctcagtacaa
     8281 gacttaacgc acgttggcgc aggtcaggca aaacgcataa cggttttgtg ggtcgttaaa
     8341 cgcggccagc ttgtcgcttt cacttttaac ggtagaggct accgaggcaa gcggtgcagg
     8401 taacatggcc tggaacgcat ctggcaacat tgcccggaca gaaccagaca tgttgtccat
     8461 cactttgtcg aagaaggtcg gttcatcaac gtagtattcc agatgccact gtttcacttt
     8521 tgccagctct gctgctttgg cgaccgcatc atcgaaatcc ccgagactat cgaccagccc
     8581 gttagctttt gcatcctgac cggtccagac gtggccctgg gcgattttat caatctgctc
     8641 cggcgtcgaa tgacgcgcat cagcaaccag cgtgataaag cgtttatagc cattctcaat
     8701 gcttaattgc atcatctgct gcgcttccgg cggcagtgcc ctggtgatag aaacatccgc
     8761 cagcggtgaa gttgagacac catcagtatg aacaccaatc gaatccagac tattttctac
     8821 ggtggtgatc acgccgaaga taccgataga accggtcagg gtgctggggt tagccacaat
     8881 gtaattagct ggcgtggaaa tccagtaacc accagatgcc gccatgccgc ccatcgatac
     8941 aaccacaggc ttacccgctg cccgggctgc tgccagttca gcgcgaatca cttcagacgc
     9001 ggtaacgctg ccgcctgggc tattaacacg caggacaatc gctttcactt tcgggtcaag
     9061 gcgagcgtcg cggatttgtg ccgcagtggt atcaccgcca acattcccct gagtttcctc
     9121 gccatccata attgcgccat tagcgaagac gacaccgatg ctgtcaccgg tatctgccgg
     9181 cgttttcaat gcgtaatcgt aataactgat ggcgcgataa tttttatcag tcttactcca
     9241 gccgaactct ttggttagag ctttttcgat ttcggcactc gatgccagtg catcgaccag
     9301 cttgttttcc agtgcatatt tcgcggtatc gccaccggtt ttggttaaac cctcaagcaa
     9361 cccttgcgcg ccagggaata cctgctgagc agggatctgc cggttagcgg caacagtatt
     9421 cagatagttt tgccacagct caccaatcca gcggctgtca gcttcgcggg ctgccggtga
     9481 catgtcatca cgaataaacg gttcaacggc agatttatac gtacccacgc ggaacacatg
     9541 ggtggaaact ttcagcttat ccagcaacga tttgtagtac agaccgttgg tagcaaaacc
     9601 gtgcaaatcc accacgcctt gcggtgacag ccaaatttta ttggcgaaac tggcgagata
     9661 atattgcccc tggctgtagt tctcgccaac ggcataaacc ggtttcccgc tgtcacgaaa
     9721 ctctttcagg gctttgccga tgtactgcat agacggctgg tcgccgcctg cgaagttttt
     9781 cagatccatc acgataccgg tgatattgcg gtcgtccttc gcctggcgaa tggtgttgac
     9841 gatatcaaac agtgagtttt cctgcagacg atcggaactg gcaccaagca gctggcggct
     9901 taatttacta aaccgctgag aactgtcggg tttatcgacg atcacaccag aaatgtccag
     9961 cagcagtgcg ccacgactgg ccgtttcttt cgaatcacca ccactgacct gcatccagat
    10021 ccccacacca accagtacga ggaaaataaa gaacaggtta agtaccattt cacggacgaa
    10081 attcagcaga cgccacgtcc atttaaaaaa tccggcaata aatcgccaaa gggttcgcat
    10141 gtattctccc aacttaaggt cacacctgtc acaggcgcaa tagatgtggt tatcgtaatg
    10201 acccaaccgg cacttgtcag caggaattgc ggactgtgct gtaacaaaat ccactctcgt
    10261 gttaattttg tgaataaata tcacgacagg agttaatcaa atggatgcac tcgaactatt
    10321 gatcaatcgc cgtagcgcct cccgcctggc tgaacccgcg ccaacgggtg aacaactgca
    10381 aaacatcctg cgtgcgggta tgcgtgcgcc ggaccataag tccatgcaac cgtggcattt
    10441 ttttgtgatt gaaggggaag ggcgcgagcg tttcagcgcc gtactggaac agggggcgat
    10501 tgctgccggt agtgatgaca aagctatcga caaagcccgt aatgcgccgt tccgcgcacc
    10561 gctcatcatc acggtggtgg cgaaatgcga agaaaatcat aaagtcccgc gatgggaaca
    10621 ggaaatgtct gccggatgcg cggtcatggc gatgcaaatg gcagccgttg cccagggatt
    10681 tggcggcatc tggcgcagtg gcgcattaac tgaaagtccg gtagtgcgtg aagcattcgg
    10741 ttgccgtgag caggataaaa ttgtcggttt tctctacctc ggcacgccgc agttgaaagc
    10801 atctacgtcg attaacgtcc cggacccgac gccgtttgta acttatttct gataattgtc
    10861 gcgaaactgt ctggattgtg agcaaaggcg caggaattca gacactctca cttatcactt
    10921 cacggaatga gggttaccat acccggattg cattgacagg agatgtccat gagcgagaac
    10981 tcgattcgtt tgacccaata cagccacgga gctggttgcg gctgtaaaat ttccccaaaa
    11041 gtgttggaaa ccatcctgca cagtgagcag gcgaagtttg ttgatccgaa tttgcttgtg
    11101 ggtaatgaga cccgcgacga tgcggcggtg tacgatctgg gcaatggcac cagcgttatc
    11161 agtaccaccg acttctttat gccgatcgtt gataatcctt tcgattttgg ccacattgcg
    11221 gcgactaacg ccatcagcga tatcttcgcg atggggggca aaccgattat ggcgattgcg
    11281 atcctcggct ggccgattaa caaactttcc ccggaaattg cccgcgaagt aaccgaaggt
    11341 ggacgctatg catgccgtca ggcgggtatt gcgctggctg gcggtcactc catcgatgcg
    11401 ccggagccga tttttggtct ggcggtaacg gggatcgtac cgaccgagcg ggtgaagaaa
    11461 aacagtaccg cacaagccgg atgcaaactg ttcctgacga aaccgctggg gatcggcgtt
    11521 cttaccacgg ctgagaaaaa atcactgctg aaaccagaac atcagggact agcgacggaa
    11581 gtgatgtgcc ggatgaacat cgcaggcgcg tcctttgcca acatcgaagg cgtaaaagcg
    11641 atgaccgacg ttacgggttt tggtctgctg ggccatttga gcgaaatgtg tcagggcgca
    11701 ggtgtgcagg cgcgcgttga ctatgacgcg atcccaaaat tgccgggggt tgaagagtac
    11761 attaagttgg gcgcagtgcc tggcggcacc gaacgtaact ttgccagcta cggtcatctg
    11821 atgggtgaaa tgccgcgtga agtgcgcgat ttgctgtgcg atccgcaaac ttctggcggt
    11881 ttgctgctgg cggtcatgcc ggaagcagaa aatgaggtca aagctacagc cgccgagttt
    11941 ggcattgaac tgacggcaat tggcgaactg gtgccagcgc gcggcggtcg tgccatggtt
    12001 gagattcgtt aattcaatgc ggttgtttat tgccgaaaaa ccgagtctgg cgcgcgccat
    12061 tgctgatgtc ctgcccaaac cgcaccggaa aggcgatggc tttatcgagt gcggtaatgg
    12121 tcaggtggtg acctggtgta tcggtcacct gcttgagcag gcgcagccag acgcctacga
    12181 cagccgctat gcgcgctgga atcttgcgga tttgccgatt gtcccggaaa agtggcaatt
    12241 acagccccga ccctccgtga ccaaacaact taacgtcatc aaacgcttcc tgcatgaagc
    12301 cagcgaaatc gttcacgccg gggacccgga tcgtgaaggg caactgctgg tggatgaagt
    12361 gctggactat ctgcaactgg caccggaaaa gcgccagcag gtgcagcgtt gcttgataaa
    12421 cgacctgaac ccgcaggcgg ttgagcgggc gctcgaccgt cttcggtcca acagtgagtt
    12481 tgtgccattg tgcgtttctg cgctggcgcg agcgcgtgcc gactggctgt acggcatcaa
    12541 tatgacccgt gcgtatacca ttctcggtcg caatgccggt tatcagggcg tactttccgt
    12601 gggacgcgtg cagacacccg tgctcgggct ggtggtgcgc cgcgatgaag agattgaaaa
    12661 cttcgtggcg aaagacttct ttgaagtcaa agcacatatc gtgacacctg ccgatgagcg
    12721 gtttaccgct atctggcaac cgagcgaagc gtgtgaaccg taccaggatg aagaagggcg
    12781 cttgttacat cgtccactgg cggagcatgt ggttaaccgc attagtggtc aaccagcaat
    12841 tgtcaccagc tataacgata aacgggaatc agaatccgcg ccgctgcctt tttcgctttc
    12901 ggcgttgcag attgaagcgg caaaacgctt tggtctgagc gcgcagaacg tgcttgatat
    12961 ctgccagaag ctgtacgaaa cgcacaagct aatcacttat ccgcgttccg attgtcgcta
    13021 tttgccagaa gaacattttg ccggacgcca cgcggtgatg aatgccatca gcgttcatgc
    13081 accagatctg ttgctgcagc cagtggtaga tccagatata cgcaaccgct gttgggatga
    13141 caaaaaggtc gatgcacacc acgccatcat cccgaccgca cggagttcag ccatcaacct
    13201 gacggagaac gaagcgaagg tctataacct gattgcccgt cagtacttga tgcagttctg
    13261 cccggatgtg gtgttccgca agtgtgttat cgaactggac attgccaaag gcaaatttgt
    13321 cgctaaagcg cgttttcttg ctgaagcagg ctggcgcacg ctgttaggca gcaaagagcg
    13381 cgatgaagaa aacgacggta cgccattgcc tgtggtggcg aaaggcgatg agttgctgtg
    13441 tgaaaaaggt gaagtggtag agcggcaaac ccagccgccg cgccatttta ccgatgcaac
    13501 actgctttcg gcgatgaccg ggatcgcgcg ctttgtgcag gacaaagatc tgaaaaagat
    13561 ccttcgtgcg accgatggtc tggggacaga agcaacccgt gccgggatta ttgaactgct
    13621 gttcaagcgt ggttttctga ccaaaaaagg gcgctatatc cactctaccg acgccggaaa
    13681 agcgttattc cattcgctgc cagaaatggc gacgcgaccg gacatgaccg cgcactggga
    13741 atcggtgctg acgcaaatca gcgaaaagca gtgtcgctat caggacttta tgcagccgct
    13801 ggtggggacg ctatatcagc ttattgatca agccaaacgt acgccggtgc ggcagtttcg
    13861 cggcattgtg gctccgggca gtggtggcag tgctgataag aaaaaggctg caccgcgtaa
    13921 acgtagtgcg aaaaaaagtc cgccagcaga tgaagtcgga agcggggcga tagcgtaagc
    13981 gagtgaatct ttcgtgctat tcgag
//
LOCUS       DAZPBM010000107        13962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPBM010000107 DAZPBM010000000
VERSION     DAZPBM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.107"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            73..2214
                     /gene="ldcC"
                     /locus_tag="ACHUZQ_002849"
     CDS             73..2214
                     /gene="ldcC"
                     /locus_tag="ACHUZQ_002849"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1026494.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2271..2660
                     /locus_tag="ACHUZQ_002850"
     CDS             2271..2660
                     /locus_tag="ACHUZQ_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1026495.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2725..4023
                     /gene="tilS"
                     /locus_tag="ACHUZQ_002851"
     CDS             2725..4023
                     /gene="tilS"
                     /locus_tag="ACHUZQ_002851"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1026496.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4072..4332)
                     /gene="rof"
                     /locus_tag="ACHUZQ_002852"
     CDS             complement(4072..4332)
                     /gene="rof"
                     /locus_tag="ACHUZQ_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1026497.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4319..4519)
                     /locus_tag="ACHUZQ_002853"
     CDS             complement(4319..4519)
                     /locus_tag="ACHUZQ_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1026498.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4685..5230
                     /locus_tag="ACHUZQ_002854"
     CDS             4685..5230
                     /locus_tag="ACHUZQ_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1026499.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5227..5588
                     /gene="arfB"
                     /locus_tag="ACHUZQ_002855"
                     /pseudo
     CDS             5227..5588
                     /gene="arfB"
                     /locus_tag="ACHUZQ_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5662..6372
                     /gene="nlpE"
                     /locus_tag="ACHUZQ_002856"
                     /gene_synonym="cutF"
     CDS             5662..6372
                     /gene="nlpE"
                     /locus_tag="ACHUZQ_002856"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1026500.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6527..7351)
                     /locus_tag="ACHUZQ_002857"
     CDS             complement(6527..7351)
                     /locus_tag="ACHUZQ_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1026501.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7404..9122)
                     /gene="proS"
                     /locus_tag="ACHUZQ_002858"
     CDS             complement(7404..9122)
                     /gene="proS"
                     /locus_tag="ACHUZQ_002858"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1026502.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9233..9940)
                     /gene="tsaA"
                     /locus_tag="ACHUZQ_002859"
     CDS             complement(9233..9940)
                     /gene="tsaA"
                     /locus_tag="ACHUZQ_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1026503.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9937..10341)
                     /gene="rcsF"
                     /locus_tag="ACHUZQ_002860"
     CDS             complement(9937..10341)
                     /gene="rcsF"
                     /locus_tag="ACHUZQ_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1026504.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10459..11274)
                     /gene="metQ"
                     /locus_tag="ACHUZQ_002861"
     CDS             complement(10459..11274)
                     /gene="metQ"
                     /locus_tag="ACHUZQ_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1026505.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11314..11967)
                     /locus_tag="ACHUZQ_002862"
     CDS             complement(11314..11967)
                     /locus_tag="ACHUZQ_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1026506.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11960..12991)
                     /gene="metN"
                     /locus_tag="ACHUZQ_002863"
     CDS             complement(11960..12991)
                     /gene="metN"
                     /locus_tag="ACHUZQ_002863"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1026507.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13179..13754
                     /gene="gmhB"
                     /locus_tag="ACHUZQ_002864"
     CDS             13179..13754
                     /gene="gmhB"
                     /locus_tag="ACHUZQ_002864"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1026508.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3389 a         3535 c         3712 g         3326 t
ORIGIN      
        1 cttcggtggc ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc
       61 caggaggaac acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat
      121 gagcccatca aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca
      181 caaaacagcg ttgatttgct gaaatttatc gaacataacc ctcgaatttg tggcgtgatt
      241 tttgactggg atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat
      301 ctcccgcttt atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg
      361 cggatggcgc tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt
      421 atgcgtcagt acaccaacga atatcttgat aacattacgc cgccgttcac gaaagccttg
      481 tttacctacg tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc
      541 gcatatcaaa aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc
      601 aaggctgatg tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca
      661 cacctggaag cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt
      721 accaacggaa catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt
      781 acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat
      841 gtagtgccag tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg
      901 cgccgtgaat ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa
      961 tggccggttc atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac
     1021 tggatcaaac agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac
     1081 acccattttc atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga
     1141 aaagtgatct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg
     1201 ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat
     1261 accaccacct cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg
     1321 cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc
     1381 aaagaggtcc agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg
     1441 ccgcaggtgg atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt
     1501 aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg
     1561 gggatggacg aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa
     1621 ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc
     1681 tttagtattg gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt
     1741 aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa
     1801 gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg
     1861 attcgtaaac acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg
     1921 atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg
     1981 ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta
     2041 ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta
     2101 ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg
     2161 aaacaggacg aagacggcgt ttaccgcgta cgagtcctaa aaatggcggg ataacttgcc
     2221 agagcggctt ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt
     2281 taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct
     2341 actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt
     2401 ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc
     2461 cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg
     2521 atgatatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc
     2581 gtgtcgatcc atacacgcaa aaacgcttca cgttctttaa cgatccggac gggctgccgt
     2641 tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca
     2701 ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca
     2761 cgccagattc tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg
     2821 gtgcagtggc ggacggaaaa tccgggcgtc actctacgcg ctatccatgt gcatcatggt
     2881 ttaagtgcta atgccgatgc ctgggttagg cattgcgaga acatctgcca acagtggcag
     2941 gtgccgctgg tggtcgagcg cgtgcaactt gcgcaagaag gactgggcat tgaggcccag
     3001 gcgcggcagg cgcgttatca ggcatttgct cgtaccctgt tacccggtga agtgctggtc
     3061 accgcgcaac atctcgacga tcaatgtgaa acctttctgc tggcgctaaa acgcggtagc
     3121 ggccctgccg ggctttcggc tatgggggaa gtttcggaat ttgccggaac acggcttatc
     3181 cgcccgttgc ttgcccgcac gcggggggaa ctggagcagt gggcattggc gcatggttta
     3241 cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgcctg
     3301 cgtgtagtgc cgttattgca gcagcgttgg ccgcattttg ccgaagcaac ggcccgcagc
     3361 gccgcacttt gtgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca
     3421 cactgccaaa cgtcgcaggg gacgctgcag attgcgccaa tgctggcgat gagtgatgcc
     3481 cgtcgcgcgg cgattatccg ccgctggctg gcagggcaga atgcaccgat gccttcccgc
     3541 gacgcgctgg tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt
     3601 ttacgttttg gcgcgtttga aatacggcgc tatcagtcgc aactgtggtg gattaaatcc
     3661 gtcactgggc aaagcgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta
     3721 ccggcggggc tgggaagtgt acagcttact gcgggaggcg atattcgccc tccgcgtgca
     3781 gacgaagcgg tcagcgtgcg tttcaaagcg ccaggattgc tgcatattgt cgggcgtaac
     3841 ggcggacgta agctaaagaa aatctggcaa gagctgggcg tgccgccgtg gctacgtgac
     3901 accacgccac tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg
     3961 caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt
     4021 taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggattc
     4081 gcttaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgcag
     4141 ctcgcgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa
     4201 atcactggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg
     4261 atgctggcag gcgagctcaa gattatcgta atcatcacaa ttgattggtt gatacgcatc
     4321 attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga
     4381 cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca
     4441 gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta
     4501 actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag
     4561 tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca
     4621 atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt
     4681 tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg
     4741 caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg
     4801 tatgatgctg cgcctgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg
     4861 tggtttgtgt gccgattatg agccggaagc gtggctgcgt aacgatcacc tgggcattga
     4921 tttgtggatt gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc
     4981 cgcaaaagtg gcgctgttta cctataatag ccgggcggcg caaatctggt ggcagcaaaa
     5041 tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact
     5101 ggcgaaagta agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg
     5161 cgtgatctgg ttatcggatg ataagaataa tctggaagtg aacttaaccg cctggcaaca
     5221 accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc
     5281 gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac ggctattcat
     5341 ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc
     5401 gccagccatc atttgatcag catgatggcg taattgtcat taaggcacag gaataccgca
     5461 gtcaggaact gaaccgcgaa gcggcgctgg cccggctggt ggcagtgatt aaagatttaa
     5521 caacagaaca aaaagcccga cgacccacgc ggcccacccg tgcatcgaaa gagcgcaggc
     5581 tggcatagaa agcacaaaaa tcaagcgtga aggcgatgcg cggcaaagta cgcagcggtc
     5641 gggaataaaa agaaggaatg gatggtgaaa aaagcgatag tgacagcgat ggctgtaatc
     5701 agcctcttta ctctgatggg atgtaataat cgggccgaag tcgatacgct ttctccggcg
     5761 caggctgccg aactaaaacc gatgccgcaa agttggcgcg gcgtgctgcc gtgtgccgat
     5821 tgcgaaggga tcgaaacctc tctgttcctc gaaaaagacg gaacatgggt gatgaatgag
     5881 cgttatctcg gtgctcgtga agaaccttcc tccttcgctt cctacggtac atgggcgcga
     5941 accgctgaca agctggtatt aaccgacagc aaaggtgaaa agtcatatta tcgggcgaaa
     6001 ggcgatgcgc tggagatgct cgatcgtgaa ggcaatccaa ttgaatcgca gttcaactat
     6061 acgctggaac cggcacaatc cagtttacct atgacgccga tgaccctgcg aggcatgtat
     6121 ttttatatgg ctgatgcggc gaccttcact gattgcgcga ccggaaaacg tttcatggta
     6181 gcgaataacg cagagctgga gcgtagctac ctggctgcgc gcggtcacag tgaaaaaccg
     6241 gtgttactgt cagtagaagg tcactttacg cttgaggcta atccggatac tggtgcgcca
     6301 accaaagtat tagcgcccga tacggcgggt aaattttacc cgaataagga ttgcagtagt
     6361 ttggggcagt aaacctgctt aaggcagaaa caaacgcaaa attgcctgat gcgctgcgct
     6421 tatcaggcct acagcatata atgcaacatt ttgaattagc acgattttgt aggccggata
     6481 aggcgttcac gacgcatccg gcattttgtg cacgaggctg gctttatcac tgagtcagcc
     6541 caacaaagcg acccgccttg atataaatcc ctggcttcag gtgcccaaca tactgtaact
     6601 gcgtttctgg cttaaacgca gagacatcgc cagtacgcat atgcatcaga tcggcagggc
     6661 taatccagcc tgattgcgcc agcgtaagcg gatgcccggc tttggcaaat gcatcagtga
     6721 caaattccga acaaaaccac gactttttgt ctccttcgcc cacactgctt aactgcgctt
     6781 tcgccaggcc gctgacgcac tgttggcgaa aatcctcgga gaacggattc agtgagcaca
     6841 tctggcgagt caccataaag ggaataaatt cgacaatgcc gcgatagtta taactgctat
     6901 ctttgatttt attggcaaac gcggtgatat ctgtggcttg ttgcggggta agatccggga
     6961 ctcgtaagac gaaaagctta tcactatgct tcatcgcttt tttaagggaa acgatctgga
     7021 cgccagcgcc tgtcgcttct gcaacgttat tatcacccag atagattgca acgtgactca
     7081 cagaggaagt gctgaagacg cggattccaa atgaggttac cccaaggctt gaggagaaca
     7141 gcaaatcgcc gggtttgaga tcaggtaccg ttatttcttt tattgattgt tcggtgaaag
     7201 agctttgatg ctggaatttg acagcccatg tttttgcctc ggcatctact gctgtggctg
     7261 atgggtctgg ctggctgata tcaacggtac aagccgaaag taaaagaaag ctggggagaa
     7321 gcaggcggca gtacgccttt ggtttatcca ttttatacaa tccatgtaaa aaagggccct
     7381 gaaattcagg accctttctg gcatcagcct ttaatctgtt tcaccagata ttcgacgatg
     7441 tcaccagtct taattaactg tttctcgccg ttgcgacgat atttatattc gatatcgtcg
     7501 ttgtcgaggt tacggtcgcc cagcacaata gtgtgcggaa taccgatcag ttccatatca
     7561 gcaaacatca cgcccggacg ctctttgcgg tcatccagca gcacttcgat accttgtgca
     7621 cgcagttcgc tgtacagttt ctcagcaagc tcttgtacgc ggaaggattt gtgcatgttc
     7681 atcggcagaa tcgccacctg gaacggcgcg atagcgtcag gccatacgat gccgcgttcg
     7741 tcgtagttct gctcaatcgc cgcagctacc acacgcgtta ccccgatacc gtagcaaccc
     7801 atcgtcagga tttggttacg gccatcttca ccctgtacgg aggctttcag tgcttcggag
     7861 tacttggtac ccagctggaa gatgtgacca acttcgatac cacgtttgat cagcagcgta
     7921 ccctggccat ccgggcttgg atcgccagcc accacgttac ggatatctgc aacttccggg
     7981 gtagcgacat cgcgatccca gttgataccg aagtagtgtt taccatcgat gttagcacca
     8041 gcagcgaaat cactcatcgc cgcaacggta cggtcaatca ccaccggaat cggcatgttt
     8101 accggaccca gtgaacccgg accggcttta accacagcac gaatttcttc ttcggtcgcg
     8161 aaagtcagcg ggcttgcaac ctgcggcagt ttttctgctt taacttcgtt cagctcgtga
     8221 tcaccgcgca ccagcagcgc aaccagcggg aagctgctgc cttcaaccgc tttaaccagc
     8281 agagtcttaa ccgttttctc aatcggcaga ttgaactgtt caaccagttc cgcgatggtt
     8341 ttcgcgttcg gcgtatcaac cagcgtcatt tcctgggtag cagcagcgcg cggttctttc
     8401 ggcgcgatag cttctgccag ttcaatgttc gctgcatagt cagaggtgtc ggagaagacc
     8461 acatcgtctt caccgctctg cgccagcacc tggaattcgt gagaggcgct gccgccgata
     8521 gaaccggtgt cggcttgcac ggcgcggaaa tccagcccca tgcggctgaa gattttgctg
     8581 taggccgcat acattgcatc gtaggtttcc tgcagggatt cctgagaagt atggaaagag
     8641 taagcatctt tcatcaggaa ttcgcgggaa cgcatgacgc cgaaacgcgg acgcacttcg
     8701 tcgcggaact tggtctggat ctgatagaag ttcagcggca gctgtttgta agagctaagc
     8761 tcgttacgaa tcaggtcagt gataacttct tcatgagttg ggccgagtac gaacggacgc
     8821 tcgccacggt caacaaaacg cagcagttcc ggaccgtact gttcccaacg accactctct
     8881 tgccacaaat cggctggctg aaccaccggc atcgacacct cgattgcacc ggcgttgttc
     8941 atctcttcac gcacgatgtt ttcgactttt ttcagaacgc gcacgccggt cggcagccag
     9001 gtatataacc cggaggccag cttgcggatc atcccggcgc gcagcatcag ctgatggctg
     9061 atcacctcgg cgtcggcagg tgtctccttg agagtggaga gcaggtattg gctagtacgc
     9121 atgttgttac ggttccagtt ggaaggtaga acaggctcaa ggcgagcctg ggacaaaaaa
     9181 agtgatttag tttaccagtg caaaagaaat gtcaaaagag aagggcgtga atttaacgcg
     9241 gttccagcgc aaagacttca aaacctgcgt cggtgacgcg ccagcgaacg ttaaaatcat
     9301 gtagccagac ggcataggtt ttgcccgttt cctcaccttt acgataggcc gggcgcgggt
     9361 cctgcgccag tacttcgcgg ataaacagcg ttaactgcgg ataacgcttc tccagcgtca
     9421 aaagctgctt ttcgacctct gcggtaaaac tcaccgccat ctctgcagct ggcgcacttt
     9481 gcgcatagct ggcactggca tcgggaagcg attcggcaaa ggggagatac ggtttgatat
     9541 ccactaccgg cgtaccatcg accagatcca gactgccgag cttcagaatc acgctgtctt
     9601 tatggcaaac aacctctttc agctctacca gcgacatacc aattgggtta gggcggaaag
     9661 tagagcgtgt tgcgaaaacc cccattctgg cgttaccgcc gaggcgcggt ggacgcacag
     9721 tcggacgcca gccgccttcc atcgtttgat gaaagacgaa aaggatccat aaatggctga
     9781 acgcttccag gccgcgaacg gcgtcggcct ggttgtaggg agcaatgaga tgcagttcgc
     9841 cgttggcgct ttttaccaga ccaggctggc gcggaacggc gaatttttct ttatagggcg
     9901 agcgaataac gcctatttgc tcgaactgga aactgctcat ttcgccgtaa tgttaagcgc
     9961 agagccgata catacagcct gacgatagca gcctagcgta ccgctggtga cttcgcagct
    10021 atgcagtaat accgcattgg ctttcatttt agaggcgttg atttgcatcc gcttacgcgc
    10081 ggttggaatg ctcggcggag agtcctgatt agaggcctgg caagagtcgc cactgacttc
    10141 accgagatcg cggaacggtt tgccgactaa ttcttctgca ttggtataaa ttcggaccgg
    10201 cgtggcgcgc ggcgctttcg gttttgcagg ctccgctttc ggctggggtg cagtgctttg
    10261 aacgggttcg acaggggatc tgcttaacat ggaacagccg cttagcatga gtgctactaa
    10321 acagatcggt aaagcacgca tagtatttcc tcaatgtatg atcaaaacgt caatattgaa
    10381 tcaggagctt gtaaaaatga caagacgggc aagcgcccgt cctgaatgat attacaaatt
    10441 gtggaaacag cctaaaaatt accagccttt aacagcgccg ccgttaaaca ctttgtttgc
    10501 tgcttcgtaa acttcatcag actgataagc ctggacgaat ttcttcacgt tctcggcgtc
    10561 tttgttatct tcacgcgtta cgatcaggtt cacgtacggg gagtctttat cttcgacaaa
    10621 gataccgtct ttcgctggag tcaggccaat ctggctggca taggtggtat tgataactgc
    10681 cagagcgatt tgcgcgtcgt ccagagatcg aggcagctgc ggtgcttcca gctcaacaat
    10741 tttcagattt tttgggttct caacaacatc aagaacggtc ggcagcaggc caacgccatc
    10801 tttcagtttg atcaagccca ctttttgcag cagcagcagt gaacgaccaa gattagttgg
    10861 atcgtttggc acggcaacct gcgaaccatc ctgcagttca tccagtgatt tgattttctt
    10921 ggagtaacca gcaatcggat aaacaaaagt gttgcctact gcgaccagtt tgtagccacg
    10981 atctttcagt tgctgatcaa ggtacggttt atgctggaag gcgttggcgt cgatatcgcc
    11041 tttgctcaat gcttcgtttg gcagaacata gtcgttgaag gttaccagct caacgtccag
    11101 gccatatttg tctttcgcaa ctttctgcgc gacttctgct acctgctgtt cggcaccaac
    11161 aatcacgccg actttaatgt ggtttggatc tttttcatcc tgaccgcagc ctaccagtgc
    11221 cagtgatcca atcagggctc ccactgccgc aaaggttttg aatttgaacg ccatacctta
    11281 ttccttcttc aattatttat gttgtgttga acgttacttg cgagtgacag cccggacgat
    11341 gcggtcgcct gcgaactgaa ttaaataaac cagaatgacc agcaatacca gtaccgtatt
    11401 catcaccgtc gcgttgtagc cgatgtagcc atactgatag ccaatctgac ctaaaccacc
    11461 ggcaccgact gcaccaccca tcgcggaata accgaccagg gtaatcaggg tgatagttgc
    11521 cgcattcacc agacccggca acgcttccgg taacagcact ttacggacga tctgcattgg
    11581 cgtggcaccc attgcgcggg aagcttcaat taacccggtt gggatctcca gcagagcgtt
    11641 ctcgaccata cgggcaataa acggtgctgc accaacggtt aacggaacaa ttgctgcctg
    11701 caaaccaatc gatgtaccga caataacgcg ggtaaacgga atcatccata ccagcaagat
    11761 aatgaacggg atggaacgga aaatgttcac aatcgcagaa acggtacgat acagcttcgc
    11821 gttagcaata atttgccccg gacgcgtgac ataaagcaga acgccaaccg gcagaccaat
    11881 cacaaagcca aaaaatccgg atacgaaggt cattgccagc gtttcccata cgccacgaac
    11941 cagcagccac atcatcggct cagacataac ccagtacctc tacttttaca tggtgttcct
    12001 gcagccaggc aatggcggct tgcgtatctt gttgtgtgcc gtgcatttca gtcagcatga
    12061 tgccgaattt aacgccaccg gcgtagtcca tctgcgcgct aataatgttg ttgttgacgt
    12121 tgaaacgacg cgcggtttca gaaagcagtg gggcatcgac cgattgaccg gtaaactcca
    12181 gacgcagcat cggcacgcag tcagtaaatg gctccgcttg cagacgttcc tggtaatctt
    12241 ccgggatatc cagatgcagg gtcgactgaa taaacttctg cgccagcggc gttttcggat
    12301 gcgagaacac ttcacttacc gtgtcctgct cgatcagttc gccattgctg atgaccgcca
    12361 cgcaatcaca aatacgcttc acaacgtcca tttcgtgggt gatcaacaga atcgtcaaac
    12421 ccagacggcg gttaatgtct ttcagcagtt cgagaataga acgtgtcgtt gccgggtcca
    12481 gcgcgctggt ggcttcatca cacagcaata ctttgggatt gctggctaat gcacgggcaa
    12541 tcgccacacg ttgtttctgc ccaccggaaa gattcgacgg gtagctatca tgcttatcgc
    12601 caagaccaac caatgacagc aattccgtca cgcgacgttt gatctcgtct ttcggtgtgt
    12661 tgtccagctc cagcggcaga gccacgttgc caaaaacagt acgcgaagag agcaggttaa
    12721 aatgctggaa aatcatacca atctggcggc gagctttggt caactcggat tctgacagcg
    12781 tggtcaattc ttggccatcg accagcacgc taccctcggt tgggcgctcc agcaggttta
    12841 cacaacgtat aagcgtactc ttacccgcgc ctgaggcacc gataacgcca taaatttgtc
    12901 cagctggcac atgcaggctg acgttgttca acgcctggat ggtgcgggtg ccctggtgga
    12961 acactttggt gatattcgaa agttttatca ttgattattt attatcgtca ttaagttagt
    13021 cgtggcatct cgaatgcctg aaacgggcaa cgccgtcaat gaaatggatg ttaaggcatc
    13081 cagacgtcta aatcaatcag gtttatgcga agagcacttt cttgcaggtc gaaacatgcg
    13141 atactagcgt cccatgcctt attaaggagc tataaaaggt ggcgaagagc gtacccgcaa
    13201 tttttcttga ccgtgatggc accattaatg tcgatcacgg ctatgtccat gagatcgaca
    13261 actttgaatt tatcgacggt gttattgacg ccatgcgcga gctaaaaaaa atgggctttg
    13321 cgctggtggt agtaaccaac cagtctggca ttgctcgcgg taaatttacc gaagcacagt
    13381 ttgaaacgct gaccgagtgg atggactggt cgctggcgga ccgagatgtc gatctggatg
    13441 gtatctatta ttgcccgcat catccgcagg gtagtgttga agagtttcgc caggtctgcg
    13501 attgccgcaa accacatccg gggatgcttt tgtcagcacg cgattatttg catattgata
    13561 tggccgcttc ttatatggtg ggcgataaat tagaagatat gcaggcagcg gttgcggcga
    13621 acgtgggaac aaaagtgctg gtgcgtacgg gtaaacctat tacacctgaa gcagaaaacg
    13681 cggcagattg ggtgttaaat agcctggcag acctgccgca agcgataaaa aagcagcaaa
    13741 aaccggcgca atgattaaaa gatgagcggt tgaaataaaa atgcattttt ccgcttgtct
    13801 tcctgagccg actccctata atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca
    13861 caaacagccg gttcggttga agagaaaaaa tcctgaaatt cagggttgac tctgaaagag
    13921 gaaagcgtaa tatacgccac ctcgcgacag tgagctgaaa gc
//
LOCUS       DAZPBM010000108        13898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPBM010000108 DAZPBM010000000
VERSION     DAZPBM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.108"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..460)
                     /locus_tag="ACHUZQ_002865"
     CDS             complement(<1..460)
                     /locus_tag="ACHUZQ_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1026509.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
     gene            complement(537..1286)
                     /gene="btuD"
                     /locus_tag="ACHUZQ_002866"
     CDS             complement(537..1286)
                     /gene="btuD"
                     /locus_tag="ACHUZQ_002866"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1026510.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(1286..1837)
                     /gene="btuE"
                     /locus_tag="ACHUZQ_002867"
     CDS             complement(1286..1837)
                     /gene="btuE"
                     /locus_tag="ACHUZQ_002867"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1026511.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(1900..2880)
                     /gene="btuC"
                     /locus_tag="ACHUZQ_002868"
     CDS             complement(1900..2880)
                     /gene="btuC"
                     /locus_tag="ACHUZQ_002868"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1026512.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(2981..3280)
                     /gene="ihfA"
                     /locus_tag="ACHUZQ_002869"
     CDS             complement(2981..3280)
                     /gene="ihfA"
                     /locus_tag="ACHUZQ_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1026513.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3285..5672)
                     /gene="pheT"
                     /locus_tag="ACHUZQ_002870"
     CDS             complement(3285..5672)
                     /gene="pheT"
                     /locus_tag="ACHUZQ_002870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1026514.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5687..6670)
                     /gene="pheS"
                     /locus_tag="ACHUZQ_002871"
     CDS             complement(5687..6670)
                     /gene="pheS"
                     /locus_tag="ACHUZQ_002871"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1026515.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(6879..7003)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(6953..6997)
                     /gene="pheM"
                     /locus_tag="ACHUZQ_002872"
     CDS             complement(6953..6997)
                     /gene="pheM"
                     /locus_tag="ACHUZQ_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1026516.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(7120..7476)
                     /gene="rplT"
                     /locus_tag="ACHUZQ_002873"
     CDS             complement(7120..7476)
                     /gene="rplT"
                     /locus_tag="ACHUZQ_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1026517.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(7529..7726)
                     /gene="rpmI"
                     /locus_tag="ACHUZQ_002874"
     CDS             complement(7529..7726)
                     /gene="rpmI"
                     /locus_tag="ACHUZQ_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1026518.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(7823..8365)
                     /gene="infC"
                     /locus_tag="ACHUZQ_002875"
     CDS             complement(7823..8365)
                     /gene="infC"
                     /locus_tag="ACHUZQ_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1026519.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8369..10297)
                     /gene="thrS"
                     /locus_tag="ACHUZQ_002876"
     CDS             complement(8369..10297)
                     /gene="thrS"
                     /locus_tag="ACHUZQ_002876"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1026520.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            10822..12720
                     /gene="espL1"
                     /locus_tag="ACHUZQ_002877"
                     /pseudo
     CDS             10822..12720
                     /gene="espL1"
                     /locus_tag="ACHUZQ_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            12892..12999
                     /gene="yniD"
                     /locus_tag="ACHUZQ_002878"
     CDS             12892..12999
                     /gene="yniD"
                     /locus_tag="ACHUZQ_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1026521.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13052..13810)
                     /locus_tag="ACHUZQ_002879"
     CDS             complement(13052..13810)
                     /locus_tag="ACHUZQ_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1026522.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         3549 a         3633 c         3162 g         3554 t
ORIGIN      
        1 ttcgtctcgc ctgccagaaa tttttctgcc aatagacatt atcaagtgag gaacgcatca
       61 ctcccttgct ggtagaggcg tggataaatt ggttgttggt atcgtaaata cctacatgca
      121 aaccattttg tccggaaccc gttttgaaaa agaccaggtc accaggcagc aactcgtctt
      181 tatcaatttg cgtgccgata gaggcttgtt ctttggtttc tcggggcagc tgcaaatcga
      241 aacgatcgcg catcgttaca accacaaatc ccgaacagtc cacaccgcgc cgcgtcatgc
      301 caccataacg atacggcgtg ccatgccagc tttgtagctg gtcgttcaaa ccggcaataa
      361 cggtaatcga atcagaaagt ctggcatttg gcggcggtgc tttatggtgg ctacacccgg
      421 ccagaaacag tgctgtgatc aaaataaggc agaaacgcat tccgtacggt tcctctgttt
      481 ttattcttgc attaatttag cgtcgtaatt acccgatttt caagatacta atgaaatcag
      541 atggtcgaaa tcagcattct gtgaccttcg atatccagac ggcgaaaatt catcccatag
      601 gcctgcgcca gatttggcgg cgtgagcacc tcttctctgc gtccactggc cagcattttt
      661 ccacctttta gcaaccacgc ccgatgcgca tgacgcaatg tgtggttgag atcgtgactg
      721 ctcatcacaa tcgccagtcc ttgctgacac agagcgctca gaattttgtc taacgcactt
      781 tgttgcgcaa catcaagact gttcatcggc tcatcaagaa gcagcaattg gcctgcggga
      841 ttggcttgtg gtgtgatttg cagcaccacc gcagcaagac gtacgcgttg ccattcaccg
      901 ccggaaagtt gattggtgct acgtccgagt ttgtcatcaa gagccagcgc ccctgcgaca
      961 tcattcagta gttcggtacg cgttttatcg tgctgatgca gtgtcagata gtgccagacc
     1021 ggcattgcaa acggcggcgt ctgctgttgt gaaagatagg cgcgatgcag cgcgagtttt
     1081 gttgcggacc atgcttccag tggttgcccc gcgaactgaa tacttccctt accgctggtc
     1141 attccggcca ttcgtgccag taaggtactc ttacccgcgc cattcggccc caccaggtgc
     1201 aggatctccc cagcccgaac ctcgccagaa agtggcccca ggcgggtaga ttccgcaaca
     1261 tcctgtaact gcatcacaat agacattatt ttgccaacgc cagtttaatg ctttccatca
     1321 caatggggtc ttccggcgtc atatccgggg aaaaacgctg gatgaccaat ccgtccctgc
     1381 caactaaaaa tttctcaaaa ttccataaaa tatcatcagg gtacagcggt gcacgacctt
     1441 tgctgaccat acgggcatag aatccgctct cttccggcgc gactgcggtc ggcgctgcgg
     1501 caatcaattt ttgatacagc ggatggcgtc cttcgccgtt aacttcaatc ttactgaaca
     1561 tcgggaacgt caccccccat gtggtggtac agtaagtttt aatctcttca tcgctgcccg
     1621 gttcttgttc cagaaactgg ttgcacggga atcccagcac cacaaaacct cgatcggccc
     1681 aggctttctg aatattctcc aactgctcat attgcggcgt taagccacac tttgaggcga
     1741 cattgacaat caacagcaca ttaccggcgt acttctccag cgtggtcact tcaccgtcga
     1801 tatctttcac tacggtcgtc agaatggaat cttgcatcgt ttctcctggg tgtggtcagt
     1861 aaaaatctta gcttttaatc atagaccgtc tttttgcggc taacgtcctg cttttaacaa
     1921 taaccagata aacaccggcg cacccaacgt tgcggtgacc acgccaatag gcagctctgc
     1981 ggcagctaat gccaggcgcg ctacaatatc ggccagcagc actgcgctcg cccctgccag
     2041 cgcgcagccg ggaagtaata cgcgatgatc ggttaaacca cacaaccgaa gaatatgtgg
     2101 gatcaccaga ccaataaagc cgatagcacc cgccagcgcc acactgacgc caaccatcca
     2161 gccggtcgct gccaccagca cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc
     2221 cgagatctcg ccaagtgcta acatattcat cggcctggac tgacaacaga tccacaacaa
     2281 cacggggatc aatgccagca tcagccagct ttgccgccag tctacgccgc caaaaccgcc
     2341 catcatccag tacatcagct gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt
     2401 cattagtgcg ctacagataa tccctaatgc aacgccagcc agcaataacc gactggtcga
     2461 aagatgacga cgggcgaaac gtaagagtat taaagtgatg ataagcgcgc cagcaatcgc
     2521 acacagccct agcgcccagt tggggagttg cccttgccca agcaataccg cggcgataag
     2581 ccccacgcct gcgccgttag agacgccaag tagtccaggt tctgccagag gattttcaaa
     2641 caacgcctgc attacagcgc cggatatagc cagcgccgca ccaaccagca atacagccag
     2701 cgtacgtggc aggcgaattt gccagacgaa cagttcgcca cgaggagtaa accagtcacc
     2761 tggcgaaatc cattgttcac cggcgcaaag gcttaagaga agcgccagca gcatcaaaac
     2821 tgacaggcat aataaccagc gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat
     2881 ggtatccgtt ctgctgaagt gtcatggcgt tgattttacg gtgactcttc gacagtgaaa
     2941 agaaaaaagg ccgcagagcg gcctttttag ttagatcaga ttactcgtct ttgggcgaag
     3001 cgttttcgac ccggcttttt aacttctgcc cgggtctgaa ggtcaccacg cgccgtgctg
     3061 taatgggaat atcctcgccc gttttcgggt tacgtcccgg gcgttgattc ttatcacgca
     3121 gatcgaagtt accaaaacca gagagtttca cctgttcgcc gttttccaga gcgcgacgga
     3181 tctcttcgaa aaacagttca accagttctt tggcatcccg cttgctaagc ccaagcttat
     3241 caaacagata ttctgacatt tcagcttttg taagcgccat aggttcaatc cctcaatgat
     3301 gcctggaatc gctcttttaa tgcctctaca catttggcga cggtagcggc aatctcctct
     3361 tcttcgagtg tacggctggt atcttgcagg atcaggctta tggcgaggct cttatacccc
     3421 tccgcaacac ccttaccgcg gtacacgtca aataagttta cgccaactac ctgatttacg
     3481 ccaactttct tacattcgga taaaatatcc gctgcgggaa cgttttctgc gaccaccacc
     3541 gcgatgtcac gacggttcgc cgggaagcga gaaatctcgc gcgcctgagg caccacgcgg
     3601 tctgcgagct tgttccactc cagttcgaac accagagtgc gaccgttaag atccagttta
     3661 cgttccagtt caggatgaac aactccaaca aaaccaatac gttccccttt cagataaatc
     3721 gctgcggatt gccccggatg cagtgccgga ttcgcttctg cacggaactc aacctcattc
     3781 agtttaccgg tcaggtcgag aacggattca agatcgcctt tcaaatcata gaaatcaacg
     3841 gtctcttttg ccaggttcca gtgctcttcg taacggttac cgcaaatcac accggctaac
     3901 atcagatcct gacgaatgcc caacggtgcc tgagtatctg gtacgaaacg cagaccgctt
     3961 tcgaaaatgc gcacacggtt ctgctgacgg ttctggttgt acaccacggt tgccagcagg
     4021 ccggtccaca gagaaagacg cattgctgac atttcaacag agatcgggct tggcagcagt
     4081 aaggcttcaa cgcctggatg gatcatctgc tgcactttcg gatcaacgaa gctgtaggtg
     4141 atcacttcct gatagccttt gtcgttgagc agcgttttca cgcgcttgag cgacaggtca
     4201 gcttcacggt gagtacccat aatcaggctt gcctgtaccg gctcatccgg gatgttgttg
     4261 tagccgtaaa cacgcgcgac ttcttcaacc agatcttctt caatctccat atcgaaacgc
     4321 cagctcggcg caactgcctg ccactcgtct ttgccttcgg tcacttcgca gccgagacga
     4381 cgcagaatgt cagttacctg ttcatcagca atatgatggc cgatcaggcg atccagtttg
     4441 ctacgacgta aagtgatggt tgcacgcttc ggcagcgttg cttcgttggt gatatcaatt
     4501 accggaccag cctcaccacc gcagatgtcg atcagcagac gggtcgcacg ttccatcgct
     4561 ttgtgctgca gtgccggatc aacgccacgc tcataacggt gagacgcatc agtatgcagg
     4621 ccatgacgac gagcacgacc ggtgatagac agcgggctga agaaagcgca ttccagcaag
     4681 acgttttgcg tttcgtcatt cacgccagag tgttcgccac caaagatgcc gcccatcgcc
     4741 agcgccttgt tgtggtcggc gatgaccaga gtgtcagcat tcagcttcgc ttcagtgcca
     4801 tcaagcagca ccagcgtttc gccctctttt gccatccgca ccacaatgcc gccttcaatg
     4861 cgatctttat cgaaagcgtg catcggctgg cccagttcga gcagtacata gttggtgacg
     4921 tcaacaactg catcgataga acggatcccg caacgacgca gtttttcttt catccacagc
     4981 ggagttggcg ctttaacgtt aatgcctttt accacacggc caagatagcg cgggcaggct
     5041 tcaggcgctt cgactgtaat cggcaacgtg tcgtcgatgg tcgcaccaac cggaacgatt
     5101 tccggttcaa ccagcggcag ctgattcagc acggcaacgt cacgcgcaac accaatgata
     5161 cctaagcagt cggcacggtt tggcgttacg ctgatttcga tggtgttgtc atcgagtttc
     5221 aggtattcgc ggatgtcagt gccaatcggc gcatctgcag gcagttcgat aatgccgctg
     5281 tgatcgtcag aaattcccag ttcagagaag gagcacagca tcccttcaga cggttcgcca
     5341 cgcagtttcg ccgctttaat tttgaaatca cccggcagaa cagcaccaat ggtagctacc
     5401 gccacacgca ggccctgacg gcagtttggc gcaccacaga cgatgtccag caggcgatcg
     5461 ccgccgacat tcacttttgt cacacgcagt ttgtcagcgt tcggatgctg cgcacactca
     5521 accacttcac caacgaccac gccgtggaag ctaccggcaa ccggttctac accgtcaact
     5581 tccaggcccg ccatagtgat ttgattcgcc agcgcatcgc tatcaatcgc cgggttcacc
     5641 cattcgcgta accacagttc actgaatttc ataatctatt cctgccttat ttaaactgtt
     5701 tgaggaaacg cagatcgttt tcgaagaatg aacgcaggtc ggtgacgccg taacgcaaca
     5761 tagtcagacg ctccatcccc atcccgaagg cgaaaccaga gtaaacttcc gggtcgatgc
     5821 caacgttacg cagcacgttc ggatgcacca tcccgcaacc cagcacttcc agccatttac
     5881 cgtttttacc catgacgtca acttctgcag aaggctcggt gaacgggaag taggaaggac
     5941 ggaagcgaat ctgcaaatct tcctcaaaga agttacgcag gaaatcgtgc agagtgcctt
     6001 tcaggttggt aaagctgatg ttggtatcaa caatcagacc ttccatctga tggaacatcg
     6061 gcgtgtgagt ctggtcgtag tcgttacgat aaacgcggcc aggcgcgatg atacgaatcg
     6121 gtggctgctg ggctttcatg gtgcggatct gtacgccaga ggtctgggta cgcagcaggc
     6181 gggtagtgtc aaaccagaaa gtgtcgtggt cagcgcgcgc cgggtggtga ccaggaatgt
     6241 tcagagcatc gaagttatga taatcgtctt cgatttccgg cccggttgcc acggtaaagc
     6301 caagctcacc gaagaaactt tcgatacggt cgatggtacg ggtaaccgga tgcagaccgc
     6361 cgttttcaat gcgacgacct agcagggaga catcaatcgt ttccgccgcc agacgcgcat
     6421 tcagcgctgc gctttccagt tccgctttac gcgcattcag cgcctgctga acctgctctt
     6481 tcgcttcgtt gataaccgca ccagctgccg gacgctcttc tggcggcagc tcacgcaggg
     6541 tcgtcatctg aagggttaag tgcccttttt tacccaaata ttcgacgcgc acattatcta
     6601 acgcggcaac atctgacgcc tggctaatgg ccgccttcgc actggcaacc agttctgcga
     6661 gatgtgacat ggttttcctc attgtgtcag tggtgacact ggttcgttgg acttagagcc
     6721 tatcccatca ggctatttta cttgccattt tggtcccggg cagtgctcga aattctcacg
     6781 tacttaaata cgctccggtt tctccgcgct ggccgtgtcc agactggctg cgacaattac
     6841 gcctgataga ctggcttttt atttttcaaa acgcgcatac aaaaaaagcc tccactggga
     6901 ggctttcagg cgctgttttc cgtttctctt ctcacgcgct agcctcctgg attcaggtgc
     6961 taaagtaaaa aaagaagcgg aaaatagcag cattcattgc ttgcgttacc ttttggtact
     7021 cttcaaaaga cctttattga aaaggctacg gcgataaaag tcaatgtttt gatggcgttg
     7081 aaacgaaaag agggagacta gctccctctt tcaactggct tatgccagag ctgctttcgc
     7141 tttttcaacc agagcggtga acgctacttt gtcgaatact gcgatatcag ccaggatctt
     7201 acggtcgatt tcaacagagg cttttttcag gccattgatg aatttgctgt aagaaatacc
     7261 gttctgacgt gctgctgcgt tgatacgcgc aatccacagt tgacggaact gacgcttacg
     7321 ttgacgacgg tcacggtaag catactgacc agctttgata acagcctgga aggcaacgcg
     7381 gtatacgcga gaacgcgcac cgtagtagcc tttagcttgt ttcaaaattt tcttgtgacg
     7441 tgcgcgtgca ataacaccac gttttacgcg agccatatgt gctctcctgt atctatattc
     7501 taattaaaaa gttaaaaacg ttaacggctt atgcgtacgg caggcacgcg attaccaggc
     7561 ccagatcgcc tttggaaacc atggctttcg gacgcaggtg acgtttacgt ttggtcgctt
     7621 ttttggtcag aatgtgacgc aggttagcgt gcttgtgctt aaaaccacct ttaccggttt
     7681 ttttgaagcg cttagcagca ccgcgtacgg tcttaatttt tggcatttta ataacttcca
     7741 cttcgcattg ttaataaacg aaacaaaggc gaacaaagcc tgtgaagccc gaaggctcca
     7801 cagacagtgc tacttgaagg ccttactgtt tcttcttagg agcgagcacc atgatcatct
     7861 ggcggccttc gatcttcgtt gggaaggatt cgaccactgc cagttcttgc aaatcgtctt
     7921 tcacgcgatt aagcacttcc ataccgattt gctggtgcgc catctcacga ccgcggaaac
     7981 gcagcgtgat tttggcttta tcaccctctt cgagaaagcg aatcaggctg cggagtttta
     8041 cctgatagtc gccttcatct gtaccaggac ggaatttaat ttccttaacc tggataactt
     8101 tttgcttttt cttctgttcc ttagaagact tgctcttttc atagaggaat ttgccgtaat
     8161 ccattatacg acaaaccggc ggctcggcgt tagggctgat ctcgactaag tctactccgg
     8221 cttcttctgc tttctccaga gcttctctca gactcacaat accaagctgc tcgccttcca
     8281 gacctgttaa gcgaacttcc tgggcgcgaa tttcgccatt gatacggtta gggcgcgccg
     8341 tttgaactcg ttttccgcct ttaatacctt attcctccaa ttgtttaaga ctgcggctgc
     8401 gaatctcttg ttgcagcttc tcgatcactt catttacgtc catgcttccc aggtctttac
     8461 cacggcgggt gcgaacggca actttgcctg attccacctc tttatcacca cagaccagca
     8521 tatatgggac gcgacgcaaa gtgtgctcgc ggattttaaa gccaatcttc tcatttctca
     8581 agtctgcttt aacacgaatg cccgcatttg atagtttttg cgtcaattcg ttaacgtatt
     8641 cagactgtga atcggtaata ttcatgataa caacctgaac tggcgcaagc caggtcggga
     8701 agaaaccagc gaattcttcg gtcaggatac cgatgaaacg ttccatcgac cccagaattg
     8761 cgcggtgaat cattaccggt accttacgct cgttgtcttc gcccacatag gaggcgctca
     8821 gacgagacgg caaggagaag tccagctgta ctgtaccgca ctgccatgca cgatcgaggc
     8881 agtcatacag ggtaaattca attttcggac cgtagaaagc gccttcaccc agttgatatt
     8941 caaacgggat gttgttttct tccagcgcaa ccgccaggtc cgcctcagca cgatcccaca
     9001 tttcgtcgct gccaatacgt ttttcaggac gagtggagag tttgacgacg atcttctcga
     9061 agccaaaagt gctgtacata tcatagacta aacggataca tccgttaact tcatcgcgaa
     9121 tttgttcttc agtacagaag atatgcgcgt catcctgggt aaatccacgc acgcgcatca
     9181 ggccatgcag cgaacctgac ggctcgttac ggtggcagct accaaactcg gccatacgca
     9241 gcggcagatc gcgataagac ttcagcccct ggttgaaaat ttgtacgtga cccgggcagt
     9301 tcatcggctt aatgcagtat tcacggttct cagaagatgt ggtgaacatt gcatctttgt
     9361 agttgtccca gtgaccggtt ttttcccaca ggacacggtc catcatgaac ggacctttaa
     9421 cttcctgata ctggtactct ttcagtttag aacgaacaaa cacttccagt tcacggaaga
     9481 tggtccagcc gtcgttgtgc cagaatacca tacccggcgc ttcttcctgc atatggtaca
     9541 ggtcgagctg tttaccgatt ttacggtggt cgcgtttcgc ggcttcttcc aggcgctgca
     9601 ggtaagcgtt aagtgctttt ttgtctgccc acgccgtacc gtaaatacgt tgcaacattt
     9661 tgttgttgct gtcgccacgc cagtaagccc ctgccgtttt cattagtttg aaatgatggc
     9721 agaaacgcat gttcggtacg tgcggaccgc ggcacatatc gacatattct tcatggaagt
     9781 acagacctgg cttgtcatca tgggcgatgt tttcgtcaag aatggagact ttgtagctct
     9841 ccccacggtt ggcgaaagtt tcacgcgctt cgtgccagct gactttcttc ttaatgacgt
     9901 cgtagttttt ctcagcaagc tcatgcatcc gcttctcgag tgcttcgaca tcttcctggg
     9961 ttaacgtgcg gtcaagatca acgtcgtaat aaaaaccgtt gtcaataacc gggccgattg
    10021 ccattttggt atgcggccaa agttgtttaa tcgcatgccc taacaggtgc gcacaggagt
    10081 gacgaatgat ctccagacct tcttcgtctt tggcggtaat gatcgacagt tgtgcgtcgt
    10141 tttcaatcag atcgcaagca tcaaccagtt cgccattaac gcgccctgcg atacaggctt
    10201 tcgccagacc tggaccaatg tccagcgcaa catccatggg gcttacagcg tggtcgtaat
    10261 ggcgttggct gccatcagga agagttataa caggcatttt atatccttaa tttgcagtgg
    10321 tgacccacac gaaagatcac atacaaagaa aaatttgttt attaacagtt cattgcgaaa
    10381 ccatctagcc aacaaatgct aaattggttc gcaatcaggt acacaaatga aagacattaa
    10441 cctctcacct tccacccggt tgaacgatgt taacactaaa aaaagggaga ttgtaccttt
    10501 ccgtttcaca ttgattcgat tcgcaattcg tttgtagtga gtacaccagc aaaacgctta
    10561 aagaaccact gataacatca cggtgcgtct gaaaggttta ctgcgagcgg atttacagca
    10621 gcataaactg aatccagcaa agttcgttgc gtgatttggc ggtagtattt taaacgctca
    10681 tgagattaat gacgaagtgg tcatatcaca atgataaaag tgacacaatt cttatagcaa
    10741 tttttcgtgc acatttcgtt ctggcgataa taattaatca tcatcctcaa aacctccaga
    10801 catatataag gcgaatagat tatgagtcaa aacgatatca ttatcagaac ccattataag
    10861 tctcctcata gattgcacat cgatagcgac ataccaacgc cttcatcaga gcctattaat
    10921 caatttgcgc gccaactcat caccctactt gatacctctg acttaagttc gatgctgtca
    10981 tactgtgtta ctcaggaatt taccgcaaac tgtcgaaaaa tttcacaaaa ttgttattcc
    11041 actgcccttt ttatcattaa ctttgccact tcacccatcc atgcagaaaa tatactcatt
    11101 acattacact ataaaaaaga aatcattccc ttatttctgg aaaccacgcc tattaaggct
    11161 aaccatttgc gaagcatact ggattatatt gaacaggaac agttaactgc cgaagatcgt
    11221 aaccattgta tgaaactgtc taaaaaaatc catagagaaa aaactataca accaacagta
    11281 aatctcaatg gtagtgcatt tttttcgcaa tctccttctg acgctatttt ttgtcgccat
    11341 ctgtcattgc aatacgccct tgattcattg agaaatggaa aaggcaaagt caacctgatt
    11401 aaacactact cctccgttga atccatacag cagcatgtcc ccttagtccg ggacgcggag
    11461 ttcagagcat tacttcgcca tcctcctgca gggagtcgcg ttatcgcgag taaggatttt
    11521 ggcttcgctt tagatatttt cttttgtcga atgatggcaa acaatgtcag tcatatgtcc
    11581 gcgattttat atatagacaa tcatactttg tcagtaaggc tacgaataaa gcagtcagtg
    11641 tatggacaat taaattatgt tgtgtctgtt tacgacccga acgataccaa cgttgccgtc
    11701 agaggcaccc acagggcagc acggggcttt ctctcgcttg ataagttcat cagttcagct
    11761 cccgatgctc agacctgggc tgataggtat gttcgcaact gtgcaattgc tattcttcct
    11821 ctattacctg agggagttcc agttgctatt ttagcgggta tcacgacacg aatgccattt
    11881 gcccctatac atccatcggc aatgttgtta ataatggcca caggccagac tcaatagctt
    11941 attacactat tcaaacagtt gcccatactc cctgaaaaag aaatcattga aataataact
    12001 gcgcagaata gcgttggtac acctgcttta tttctggcta tgatgaacgg acaaactgac
    12061 aacgtaaaaa tatttatgca agaaattcag tcactggtat acaatcatat cattcatgaa
    12121 gataatctgg ttaaattact gcaaactaaa agtgctaacg aaacacctgg actctatatc
    12181 tccatgttgt atggattcga tgaaataatc gatatctttc tgaatgcatt aaccactcca
    12241 atagcacaag aacttttaaa caaaaaaatg gtgatgagta ttttagccat gaaaatacat
    12301 gatggtgagc caggattata cgccgcaatg gaaaataatc accctttgtg tgtcacacgg
    12361 ttcctctcta aaattaatgg catcgccttt aaatacaagt tgagcaaagc taacatcatg
    12421 gatttattaa aaggagctac agcacaggga acccctgcat tatacatcgc catgagcaag
    12481 ggtaatgaag acgtcgtgtt atcttatata tcgacgctgg gtgcttttgc aaaaaaacat
    12541 tcttttagtc aacatcagtt atttacacta ttggccgcta aaaatcatga caacatgtca
    12601 gctgttcata tagccattca tcataagcat tataaaactg tagaaacata ttatgctgct
    12661 ataaatgtca tcagccaaag cctgagtttt agtgctgatg aaataaagac gtatttataa
    12721 ccagctattt acgatatata tcgatacata attttatttt tttcataaaa cgcagaaaca
    12781 gtgaccatgt gcacaacgcc acccgacggc tgtaataaca aaaaatccct tgatgcctat
    12841 cccttttgtt acactccgtt atcacgcaca agagatatgc aggacactgg tatgccgact
    12901 aaacgctttg ataaaaaaca ctggaagatg gtggtggtgc tactggcaat ctgtggcgct
    12961 atgttgttgc tacgttgggc agcaatgatt tggggctgag cagtgacaaa cgaagacagc
    13021 cagaccgcat aacacggtcc ggcagataat tttacatgct gtagcccagc gataaggttg
    13081 tccgacgatc ggtatgttcc ggtgcagact ctggaggttc agagttccat gtgacgttgt
    13141 aagccacctt cagtccaaaa tgttcattaa tagcaacatt taatgcgctt tcggagttca
    13201 gcgttgtatc ttccgcgcca aagaccgaaa caccctgcgt aaatttagcg ttgtcagtca
    13261 actgccatgc ataagccccg gaggcatagc ccagcggctg ggtttcactg gcattgtcgg
    13321 tatatttgtc gtaacgcaca cctgggccga attcaaagcg gaaactgtgt accgggccat
    13381 tcaaaaactg gcgaccataa cccgcggtca acacatcgcg ctctcgatag ccgttataac
    13441 ggtctgtcag ccagcttgcc tgaccaaata aatagtcata atcagttaaa ttaaaacggc
    13501 tacgcccgcc cgccgcatat ttttctgaag aacgctcatc attagaagaa gtattactgg
    13561 cgttccccca cagcgaccag gcagtggttt gtccatacca ggtcatggtg gtgtcagccg
    13621 taagtgagga gcttttcgta ttgcctgatt gtgcaagata tcctgcgttc agattacctt
    13681 cgaaaggttt tttagcgctg gcaggatcgt ccatgacagt aaaaacggaa tcatcggcag
    13741 ctgcattcag tgacgcaaac atgccccccg ccaacataac gatggcagga actgtcttca
    13801 aaagcttcat ttatcaagag tccgtacaac aaaaaaagag accatcgcgg tcccggaaac
    13861 tttcttaagg atcaaagatt agcgtccctg gaaaggta
//
LOCUS       DAZPBM010000109        13853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPBM010000109 DAZPBM010000000
VERSION     DAZPBM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.109"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHUZQ_002880"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHUZQ_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHUZQ_002881"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHUZQ_002881"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1026523.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHUZQ_002882"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHUZQ_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1026524.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHUZQ_002883"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHUZQ_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1026525.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="ACHUZQ_002884"
     CDS             4177..5415
                     /locus_tag="ACHUZQ_002884"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR1026526.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHUZQ_002885"
     CDS             complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHUZQ_002885"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1026527.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHUZQ_002886"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHUZQ_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1026528.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHUZQ_002887"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHUZQ_002887"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1026529.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHUZQ_002888"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHUZQ_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR1026530.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHUZQ_002889"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHUZQ_002889"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR1026531.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHUZQ_002890"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHUZQ_002890"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR1026532.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHUZQ_002891"
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHUZQ_002891"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1026533.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHUZQ_002892"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHUZQ_002892"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1026534.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHUZQ_002893"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHUZQ_002893"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1026535.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHUZQ_002894"
     CDS             complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHUZQ_002894"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1026536.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3400 a         3507 c         3688 g         3258 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc atccaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaacgtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tggtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca acg
//
LOCUS       DAZPBM010000110        13465 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPBM010000110 DAZPBM010000000
VERSION     DAZPBM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13465
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.110"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1726
                     /gene="adeD"
                     /locus_tag="ACHUZQ_002895"
     CDS             <1..1726
                     /gene="adeD"
                     /locus_tag="ACHUZQ_002895"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1026537.1"
                     /translation="AEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRY
                     IAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVI
                     CDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAW
                     RDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIE
                     NCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWE
                     IAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSD
                     ARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGK
                     RYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGLNE
                     GALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLM
                     STDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDVEKFA
                     FTTLEVTE"
     gene            complement(1771..3162)
                     /gene="uhpT"
                     /locus_tag="ACHUZQ_002896"
     CDS             complement(1771..3162)
                     /gene="uhpT"
                     /locus_tag="ACHUZQ_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1026538.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3300..4619)
                     /gene="uhpC"
                     /locus_tag="ACHUZQ_002897"
     CDS             complement(3300..4619)
                     /gene="uhpC"
                     /locus_tag="ACHUZQ_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1026539.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4629..6131)
                     /gene="uhpB"
                     /locus_tag="ACHUZQ_002898"
     CDS             complement(4629..6131)
                     /gene="uhpB"
                     /locus_tag="ACHUZQ_002898"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1026540.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6131..6721)
                     /gene="uhpA"
                     /locus_tag="ACHUZQ_002899"
     CDS             complement(6131..6721)
                     /gene="uhpA"
                     /locus_tag="ACHUZQ_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1026541.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6796..7086)
                     /gene="ilvN"
                     /locus_tag="ACHUZQ_002900"
     CDS             complement(6796..7086)
                     /gene="ilvN"
                     /locus_tag="ACHUZQ_002900"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1026542.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7090..8778)
                     /gene="ilvB"
                     /locus_tag="ACHUZQ_002901"
     CDS             complement(7090..8778)
                     /gene="ilvB"
                     /locus_tag="ACHUZQ_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1026543.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(8884..8982)
                     /gene="ivbL"
                     /locus_tag="ACHUZQ_002902"
     CDS             complement(8884..8982)
                     /gene="ivbL"
                     /locus_tag="ACHUZQ_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1026544.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9547..9636
                     /gene="tisB"
                     /locus_tag="ACHUZQ_002903"
     CDS             9547..9636
                     /gene="tisB"
                     /locus_tag="ACHUZQ_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1026545.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9760..9834)
                     /gene="ysdE"
                     /locus_tag="ACHUZQ_002904"
     CDS             complement(9760..9834)
                     /gene="ysdE"
                     /locus_tag="ACHUZQ_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1026546.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9916..11100
                     /gene="emrD"
                     /locus_tag="ACHUZQ_002905"
     CDS             9916..11100
                     /gene="emrD"
                     /locus_tag="ACHUZQ_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1026547.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11108..11605)
                     /locus_tag="ACHUZQ_002906"
     CDS             complement(11108..11605)
                     /locus_tag="ACHUZQ_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1026548.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11602..11964)
                     /locus_tag="ACHUZQ_002907"
     CDS             complement(11602..11964)
                     /locus_tag="ACHUZQ_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1026549.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11954..12301)
                     /locus_tag="ACHUZQ_002908"
     CDS             complement(11954..12301)
                     /locus_tag="ACHUZQ_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1026550.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12410..12859
                     /gene="yidI"
                     /locus_tag="ACHUZQ_002909"
     CDS             12410..12859
                     /gene="yidI"
                     /locus_tag="ACHUZQ_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1026551.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12906..13064)
                     /locus_tag="ACHUZQ_002910"
                     /pseudo
     CDS             complement(12906..13064)
                     /locus_tag="ACHUZQ_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13108..>13465)
                     /locus_tag="ACHUZQ_002911"
     CDS             complement(13108..>13465)
                     /locus_tag="ACHUZQ_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1026552.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3164 a         3765 c         3530 g         3006 t
ORIGIN      
        1 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
       61 tgataatgtc tctattctcg acctgatcaa tggcggagaa atttccggcc caattgtgat
      121 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatgctccgg ctttgcagcg
      181 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
      241 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
      301 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
      361 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtacc
      421 cgccctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
      481 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattag
      541 cgggcagaat gcgctgctcg ataaactgga tgcatttcgc cacctgacgc tggacggtca
      601 ctgcccgggt ttgggtggta aagaacttaa cgcctatatt actgcgggta ttgaaaactg
      661 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
      721 gatccgcgaa gggtccgctg cccgcaatct caacgcgctg gcaccgttga tcaacgaatt
      781 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
      841 aggacacatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
      901 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
      961 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     1021 gcagcaggta ctggtgaaag gcgagccgat tgatgcgcaa accttacagg cggaagagtc
     1081 ggcgagactg gcacaatccg ctccgccata tggcaacacc attgcccgcc agccagtttc
     1141 cgccagcgac tttgccctgc aatttacgcc cggaaaacgc tatcgggtca ttgacgtcat
     1201 ccataacgaa ttgattacgc actcccactc cagcgtctac agcgaaaatg gttttgatcg
     1261 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcttg
     1321 tggtttgctt ggcggctttg gactgaatga aggtgcgctg gctgcgacgg tcagccatga
     1381 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     1441 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaggtcc aaagtcatct
     1501 gccgttgccc attgccggcc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     1561 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     1621 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     1681 tgatgtcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaagcccggc
     1741 gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct gaattttttt
     1801 ctcgcggcgg attttgcgtt cttccatcac cgccactatc gccatcaggc agatacaacc
     1861 aatcgcggcg atatcaagtg cggcgaaggt gcctgcccag ccggtaaggc cgaataccgg
     1921 cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa tcaggtaagc
     1981 aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa agccaacagc
     2041 agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa agagagaagc
     2101 cagatagata tattcgttac tggcatgttg atacacaccg agcgtggcga taatcagcgc
     2161 cagcgcgatg caggccacca ggccacggcg accgttcgct aggtcagaga gccagcccca
     2221 cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa tcgccaccgc
     2281 tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga taccaatacg
     2341 taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt ttttcagcac
     2401 atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt tgtcctcttc
     2461 gctgatctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt ccggggagtc
     2521 gctgccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg ggaagataaa
     2581 catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca cgcctgctgc
     2641 gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc cgcgtttacg
     2701 acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac tctggaaaaa
     2761 gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc tgcccatact
     2821 ggcactgaag cccagcatac aaatggcaga caggatcagc atgaacggca ggaattgttt
     2881 ggtgtttttg ccgtcggcgt agtaggaaac cagcgtttta cccacgccat aagtgatgga
     2941 gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg agatcatgtc
     3001 gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc cgataaagac
     3061 caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt cgagcggaag
     3121 gtccagggtc ggcttgcgaa cctggtttaa gaaagccagc atgggttact cctgaaatga
     3181 atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga gatagcgtcc
     3241 aggcaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg tgagatgcat
     3301 cacgcttcgc gcggtgtctg ggcgttcaaa aagggcagta acagcagtgc ggaaatcccg
     3361 gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag tactttcgcc
     3421 agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa ccccgtcgcc
     3481 gcccctgccg cctctttgtg ggaacactct gccgccgcca taccgattaa catctgtggg
     3541 ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta gctggcaaat
     3601 ggcatcagcc acagggagcc gactgaaagc aaaattccgg cggcgaaaat caaattcatc
     3661 ggccctcggt tgccgttaaa caatttgtcc gagccccaac ccgctaccag cgcaccgata
     3721 aatccaccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc gacgcccagc
     3781 gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac atagaccagc
     3841 acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt ggtgaggatc
     3901 tctttacgcg tcagccctgc cccttcttgt tgttgagcta tctccagcgc gtcgtgtcgc
     3961 cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgcagccg ccagcagaga
     4021 aaaattccca cgacgatcgc catacaacca gcaatcatca tcccggcacg ccagccgtaa
     4081 tgtagtgcag ccgctgccat cacaatggga atgagtgcgc cgccgacgtt atgcgccgtg
     4141 ttccataatg cccaccaacc accgcgctcg gtacgtgaat accaggccgt taacagacgc
     4201 gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag tacggcaaac
     4261 gcccatagcg acgtcgagaa gccaaacaga atgttgataa tgcccgtggc gataagccct
     4321 atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac aaacttcgac
     4381 acgccatagg taatgtaaaa cagggtcgct aacaggccga tatcgctacg gctgagcacg
     4441 ccgttagcaa ggatttctgg tacggcggcg ttaaaacttt tccgcgtgaa gtaaaacagc
     4501 gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata gcgggcatca
     4561 atttcatatt tatcagccat taatggcgca tcggcaggcg ctttcagaaa cggcaacatc
     4621 atcaaacctt agacatagcg ttgaggtaga gaaacgctga cacgcgtgcc gtgcagacag
     4681 gaaatgtgta atgtgccacc cagcgccgtt acgcgctcgc gcattccggt gaggccaaaa
     4741 ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaataac cagcatcaac
     4801 cgttcatcct gctgccagcc ttgcagggtg accgcgctgg catcagcatg tttcacaatg
     4861 ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc gcttaacgct
     4921 gattcatcga ttcgccattc gagatggctg acaataccgc gcccttccag ctccatttcc
     4981 cgcatcagtg agcggatggc ctgctccagg gtgagatcat ccaactggcg cggacgtaac
     5041 cgacccaaca aacggcgcac cgcgtcgtaa acgcccagcg atagttgttc gatgagttgc
     5101 ccgctctgct tcacgctggc gttatctgcc gccagccgct gaacaatgcc cgcctgagta
     5161 cgaatagcag tgatggtctg gccgatatca tcatgcagct cacgcgccac atcacggcgc
     5221 acgctctctt cggtttccag caaccgttca gccagatgct gattgcgcgc cagttccttt
     5281 tgcagcgact ggttaagttc acgcaaccgc tggatgccag cgccaagcaa caaccctgtc
     5341 agactttgca ccagcagcga gagcaataaa tccaccggat gatcgcgcca ggtttgactg
     5401 gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ccccttgcca accatagtgc
     5461 caggccagcg cgataatcgg cagcaccaga cagaatggcg taaagcgcga cagttcgtcc
     5521 ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca gaccagatgt
     5581 cgcccgcgcc agttgattgg ctgagaaacc agtgacgggc cgagcggcag ccaggtgtta
     5641 ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcaggcc gccagttaaa
     5701 gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag cgactgcaac
     5761 aacgccgccg ccgttaacgc cgccccctgt aacagcaagg tgcgccagtc acgctgatgg
     5821 cgatagcgcg agatcagcgc tacgggcagt aacgtcagta aactaccgat catcaataac
     5881 ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca ctccgcaccc
     5941 agcaatacgg gccagtatcc gcgcgggcat tgcagcatta gccccagacg cagaccaaac
     6001 ggaaataaca gcaccgccat atcagggcgc tcaaccagat gcaggctgat actccacagg
     6061 caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa gcgggagaac
     6121 aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg ttactgacgc
     6181 ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac aagcccagtt
     6241 cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc tggcgttcac
     6301 gtttggttag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc ggcgtcagat
     6361 aacagccacc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg ctacagcgtt
     6421 tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca ggactgtcgt
     6481 gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc tccagaccgg
     6541 agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc ggcagccccg
     6601 ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt tccagcccca
     6661 gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca acggtgatca
     6721 tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc gcgatgttgt
     6781 tcaagccttg agcggttact gaaaaaacac cgcgatcttg ttaaacatcg tcggatcgga
     6841 ctgattacgc tgcactttca cgacatcttc cagcttatcg atttggctta tcatctgctc
     6901 cagacgctgg tcgtcattga ccagtagcca gatatggctt ttgtcgctgt cctgaatcgg
     6961 cagacaaaga atgccttcaa cgttaaaagc gcggcgggca aaaaggccac aaacgtgggt
     7021 cattacgccc ggatggttgc gaacggtgag ctccagaatt acgttgtcat gagttgtgtt
     7081 ttgcatggct tattcctcca ccatttcagt attcgccgca cctggcggca ccatcgggta
     7141 aactttttct tcggcatcaa tgcgcacatg gatcagcgcc gggctagggc gattgatgat
     7201 ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa
     7261 tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa aaacgccttg
     7321 ctcgtagaac agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcagaat
     7381 gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag
     7441 gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg ccgcgccaat
     7501 cgccgcaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc actggcgtgg
     7561 gcgattgagc ggataagctt gcgcggtcca catctgatgc tgacccacat cggtggtgat
     7621 aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg
     7681 atcgcacgct ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta ccaactggtg
     7741 ccactctgca cgcggttgcg cttccacctg cgggatcaat tgcgccagca cgtcatcaac
     7801 atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcgc gatcgatatc
     7861 gacatgaata attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc
     7921 aaaacgcgca ccgagcacaa tcaacaaatc cgcctcctgc aaaatatagt tggtactgcg
     7981 cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca gcatgcccag
     8041 cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac gcacccgcgc
     8101 aggcgcattg atcacaccac cacccagata aagcaccggg cgtttggcag cgttaatcat
     8161 tgtagctacg tcacgaatgc tttcttcgct aaaggcgggt gcagcggctt tttctgccac
     8221 cgcgggctga gcttcaatct caaaaaccgc cgtttgtaca tccttaggaa tgtctatcca
     8281 caccgggcca gggcggcctg attgcgcaat gcggaaggca tcgctcatga cctgcgggag
     8341 ttcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt
     8401 gtcgacttcc tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca
     8461 aatcagcggg atggagtcca gccgcgcatc ggcaatggcg gtcaccaggt tagtcgcacc
     8521 cggtccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc
     8581 gataaagccc gcgccctgtt catgacgggc cagaatatgg cggatttgcg tgctttggct
     8641 taaggcatcg taaacaggca ggatagaacc gcccggaatg cctgtcacaa tcttaatgcc
     8701 ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcaatgt
     8761 tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggtgct
     8821 taaacgaaaa accccgcccg gtttgcgccg acggggtttt ggaatcgtgt gttgttccag
     8881 tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg
     8941 cgcagttggt agtagttttg cgttgaacat ggaagtagtc attggggacc ttgttggttt
     9001 tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga aaatttttca
     9061 gcaagggaaa attgaggggt tgatcacgtt ttgtactgaa ttgcagataa caaaaaaccc
     9121 cgccggagcg aggtttcgtc agtcgcccgc ggctggtaac cgcaaagcac actgtattat
     9181 gtcaacacag aaagtatacg tgttccgcgc agaacgcgca atttcggcac gaattttgac
     9241 gtatttagtg catagttgag tatcgatcac agtttgcgtt ttgtccaaat attactgttt
     9301 atttatacag taaacttcta taatatcact gtacgcaatg tgttatgcgg gggccgcatc
     9361 gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc ttaaccccgc
     9421 gtgagcacac tgtgttatgt caacaagcac aaagtttctc cttgagatac cgcgtgcaca
     9481 acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga accacaggag
     9541 acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt tgcagcactg
     9601 caactgcttg atgctgttct gaaatacctg aagtaattca gattcaagtc gcaccaaagg
     9661 ggagcgggta accgctcccc ttttatattt agcgtgcggg ttggtgtcgg atgcgatgct
     9721 gacgtatctt atccgcccta ccatctctcc cggcaacatt tattgccgct tttgtttaca
     9781 tattctgccg ctaaacaact ccccattcct ggcgtatatc tggctaacat tcatcaatgt
     9841 gatagattcc tctcccgcat ttatgggaat gcgtagtgac ttactctaat tatttttata
     9901 aaagcatccg tgataatgaa aaggcataga aacgtcaatt tgttattgat gttggtatta
     9961 ctcgtggccg tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg
    10021 cgcgatctca acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact
    10081 tacggtgtct cacagctgtt ttatggcccg atttccgacc gtgtgggtcg ccgaccggtg
    10141 atcctcgtcg gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt
    10201 ttgacggtat tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta
    10261 atggcgcgta ctttgccgcg agatttatat gaacggacac agttgcgcca tgctaacagc
    10321 ctgttaaaca tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg
    10381 gatacgatgt ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg
    10441 accttcagta tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc
    10501 ctgcttacca gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg
    10561 ctgattggcg gtctggccgg gattgccgcc tttgaagcct gctccggcgt gctgatgggc
    10621 gcggtgttag ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggca
    10681 gcgttttttg gcgcatggtt tgccggacgt cccaataaac gcttctccac gttaatgtgg
    10741 cagtcggtta tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc
    10801 gtgatgaatg tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg
    10861 ctgtttcctc tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct
    10921 ggcgcgctgg tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg
    10981 atgttgccgc aaaccggtca gggcagcctg gggttgttga tgaccttaat gggattgttg
    11041 atcgtgctgt gctggctacc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa
    11101 gcgcacgtca ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat
    11161 cacgcatcac ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa
    11221 gcacgctgtc tggcgtctcc tgccgagcgt gcgggcacag gcgatgccag tcgcacgcct
    11281 ggcagtcaca gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa
    11341 aaagctcaat cgagatacga ctgatatctg cccgcaccca acactcgaac agggcaatta
    11401 aaaatttccc ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa
    11461 attcaagata gggaatgagc tgtacggagc ggatgccccg ctgtttgaaa ttgtgataaa
    11521 aatcggcagg ttgcagagca tccttgtact ctacaaccag tttatgcctg tcctcttcag
    11581 catcaatgac ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat
    11641 atgcgttaca gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc
    11701 acggactacg tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg
    11761 ggtgtagtgc cagaggatca gcgccacgat ggcgagaatg ccaatcgaaa tccagaataa
    11821 catgcccgcc tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag
    11881 catggtacga aaccacgcca gtgatgtgcg ctccggctgc agtcctgggt cggcaatgcg
    11941 tctggctttg cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacgacca
    12001 tcaatattaa gctaatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca
    12061 tcgctttttc attgcgtaac cagcgcaggt agccatacat tgctaacccg ccggaaaaca
    12121 gacacaacaa caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt
    12181 gatcaagccc aacccccgcc gccagaaatc ctagtgcggt acggatccac gccagaaaag
    12241 tacgctcatt agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca
    12301 ttgttgctcc tttgcaattt cactaactct ctacctttag cggttttttt taagaatttc
    12361 gtccgcccgt ttcctgatat gcttttgcca tccgctacaa ggaggtcaga tgggtatcat
    12421 agcgcaaaat aaaatcagtt cattgggcat gctgttcggt gccatcgctt taatgatggg
    12481 gattatccac ttttcattcg gccctttttc cgcaccaccg ccaacgcttg aaagcatcgt
    12541 ggcggacaaa acggctgaaa ttaaaagagg tcttctcgcc ggaataaaag gcgaaaaaat
    12601 taccaccgtg gagaaaaaag aggacgtaga tgtcgataaa atcctcgatc aaagcggtat
    12661 cgcactggcc attgctgcgc tactttgcgc atttgttggc ggtatgcgta aggaaaatcg
    12721 ctggggcatt cgcggcgcgc tggtttttgg cggcggaacg ctggctttcc ataccttgct
    12781 ttttggtatc ggcatcgtgt gcagcatttt gctgatattc cttatctttt ccttcctgac
    12841 cggcggttcg ctggtttaag cagcctgaat aatgcccgcc gggtgatcat cagcgggcat
    12901 tatcgtcaga acttctgttt tttctcttcc accttcaccc cttgtgtcgg taggccggtg
    12961 tcatagtcac gtaccaccgg cgaatagcca tcttgtgggc gtggacgaaa tgcccccatc
    13021 cagcgcggcc gtgcatcttt acgccacgga cgcaggctcc attgttgaca tcctccacgc
    13081 cctgaaggac gtggacccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc
    13141 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    13201 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    13261 tctgaattta ctttgtgaca ggcgattttc tttcgctgta tgaccacagc acacgcaacg
    13321 ctggcttgtg tacgctggcg gaacagcaag cacctgacca ccacgccaga gctgcttgta
    13381 cgcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    13441 ttttgcccgg acattgcgcc ccggc
//
LOCUS       DAZPBM010000111        13265 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPBM010000111 DAZPBM010000000
VERSION     DAZPBM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.111"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUZQ_002912"
     CDS             complement(<1..312)
                     /locus_tag="ACHUZQ_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR1026553.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUZQ_002913"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUZQ_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1026554.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUZQ_002914"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUZQ_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1026555.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHUZQ_002915"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHUZQ_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1026556.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHUZQ_002916"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHUZQ_002916"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1026557.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHUZQ_002917"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHUZQ_002917"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1026558.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHUZQ_002918"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHUZQ_002918"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1026559.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHUZQ_002919"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHUZQ_002919"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1026560.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUZQ_002920"
     CDS             complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUZQ_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1026561.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUZQ_002921"
     CDS             complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUZQ_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1026562.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUZQ_002922"
     CDS             complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUZQ_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR1026563.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12702..>13265)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUZQ_002923"
     CDS             complement(12702..>13265)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUZQ_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1026564.1"
                     /translation="NIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSDTAD
                     QIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVG
                     TPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEMKFI
                     ARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3478 a         3386 c         3001 g         3400 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctagagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg tccaaatcgc cacctgtact ggcggtgttg agatcggtga tctcaaaagc
    10981 accttgtgga acggcagact gataaatcac gtagccattc tgccgaatga tgacggtggc
    11041 gttggacttt gcaatcccac gaaccacagg agcaaagcca ttctggctgg cgggtagcat
    11101 atcgttatca gtatacaaac gcgcgccgcg aatttgcgta ctgtcgaaaa tatcgctcgc
    11161 cgtccacgta tcaccaatca taatctggct tcttaacgcg gcaatatcgc gctgtatcca
    11221 ggtactaatg gagttgtatt ccgcacggcc atcgctggtg tttaaagtac tgtaattacg
    11281 tagccgccac ggtccaatat tcagtccatt gcgcagattg agataactgg tatctgtctc
    11341 ttgtgaacca taatctgaac gtagtgtatg tgaaccagaa aaatcgtagt tgatggtgag
    11401 cgcgttaatc ccctcatccc aacgcgtggg gctgatgtaa tcataagcca tcgcgtccat
    11461 gtagatttgg ggaatggata tattcagctg caatttatca ctgtcaaact gccaggatga
    11521 gccttcaata cgtggagcag gatcgaggca aaccgtctca tccacatcat tgaacgattt
    11581 gagcgcatca acttttatgc caaaggagga cagtgttttt ttaatggcaa taaagcaagc
    11641 gtgcagatca gcatctggat tatcctgaac aaatgtcaca tcctgacgtt caaacatttt
    11701 accgttgata aaaatatcaa cgctataaac acctgggggc atcaggtttg cccgattaaa
    11761 ctggcgaata tcgactccgg attgatggtc gatttccaga agcgccggat tgaatacact
    11821 gacctcggcc atcaggtttt gcgagaaaat cagacacgtc cctgccgtga ggccaaccac
    11881 tattctggtc gtcatcataa tacgcctatc cttatccctt gcttacagcg tcatttttat
    11941 cgggtcactg actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga
    12001 attagcaggt aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc
    12061 atggccatca acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat
    12121 cagatttcgc ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg
    12181 gatatttgct ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt
    12241 cgtcgctttg ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc
    12301 gcgatcatca ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga
    12361 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
    12421 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
    12481 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
    12541 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
    12601 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
    12661 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
    12721 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
    12781 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
    12841 ttataaagtt taaccagcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
    12901 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
    12961 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
    13021 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
    13081 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
    13141 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
    13201 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
    13261 atatt
//
LOCUS       DAZPBM010000112        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPBM010000112 DAZPBM010000000
VERSION     DAZPBM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.112"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHUZQ_002924"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHUZQ_002924"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1026565.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUZQ_002925"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUZQ_002925"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1026566.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUZQ_002926"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUZQ_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1026567.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUZQ_002927"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUZQ_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1026568.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHUZQ_002928"
     CDS             complement(4036..5568)
                     /locus_tag="ACHUZQ_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1026569.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUZQ_002929"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUZQ_002929"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1026570.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUZQ_002930"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUZQ_002930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1026571.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHUZQ_002931"
     CDS             complement(8663..10399)
                     /locus_tag="ACHUZQ_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1026572.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUZQ_002932"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUZQ_002932"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1026573.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUZQ_002933"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUZQ_002933"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1026574.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHUZQ_002934"
     CDS             complement(12121..12855)
                     /locus_tag="ACHUZQ_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1026575.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="ACHUZQ_002935"
     CDS             13056..>13260
                     /locus_tag="ACHUZQ_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1026576.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3367 a         3373 c         3487 g         3033 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aatcagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAZPBM010000113        13231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPBM010000113 DAZPBM010000000
VERSION     DAZPBM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.113"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            66..422
                     /locus_tag="ACHUZQ_002936"
     CDS             66..422
                     /locus_tag="ACHUZQ_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1026577.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(473..1365)
                     /gene="yhaJ"
                     /locus_tag="ACHUZQ_002937"
                     /pseudo
     CDS             complement(473..1365)
                     /gene="yhaJ"
                     /locus_tag="ACHUZQ_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1470..2171
                     /locus_tag="ACHUZQ_002938"
     CDS             1470..2171
                     /locus_tag="ACHUZQ_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1026578.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2194..2358
                     /gene="yhaL"
                     /locus_tag="ACHUZQ_002939"
     CDS             2194..2358
                     /gene="yhaL"
                     /locus_tag="ACHUZQ_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1026579.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2492..3802)
                     /gene="cyuA"
                     /locus_tag="ACHUZQ_002940"
     CDS             complement(2492..3802)
                     /gene="cyuA"
                     /locus_tag="ACHUZQ_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1026580.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3830..5161)
                     /locus_tag="ACHUZQ_002941"
     CDS             complement(3830..5161)
                     /locus_tag="ACHUZQ_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1026581.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5436..6800)
                     /gene="tdcG"
                     /locus_tag="ACHUZQ_002942"
     CDS             complement(5436..6800)
                     /gene="tdcG"
                     /locus_tag="ACHUZQ_002942"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1026582.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6872..7261)
                     /locus_tag="ACHUZQ_002943"
     CDS             complement(6872..7261)
                     /locus_tag="ACHUZQ_002943"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1026583.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7275..9569)
                     /gene="tdcE"
                     /locus_tag="ACHUZQ_002944"
     CDS             complement(7275..9569)
                     /gene="tdcE"
                     /locus_tag="ACHUZQ_002944"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1026584.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9603..10811)
                     /gene="tdcD"
                     /locus_tag="ACHUZQ_002945"
     CDS             complement(9603..10811)
                     /gene="tdcD"
                     /locus_tag="ACHUZQ_002945"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1026585.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10837..12168)
                     /gene="tdcC"
                     /locus_tag="ACHUZQ_002946"
     CDS             complement(10837..12168)
                     /gene="tdcC"
                     /locus_tag="ACHUZQ_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1026586.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12190..13179)
                     /gene="tdcB"
                     /locus_tag="ACHUZQ_002947"
     CDS             complement(12190..13179)
                     /gene="tdcB"
                     /locus_tag="ACHUZQ_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1026587.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3356 a         3597 c         3144 g         3134 t
ORIGIN      
        1 tacgaatagc atttacacaa tacccggaat agactttcgt cacttttaat taaagggatg
       61 tttttatgca gtggtattta tccgtattaa aaaattatgt tggtttctct ggtcgcgcac
      121 gtcgtaaaga gtactggatg tttactctga ttaacgccat cgtcggcgct attatcaatg
      181 tcattcaatt gattttaggt ctggagcttc catatctgtc tatgctttat ttgctggcaa
      241 ccttccttcc cgtccttgcg cttgctatcc gtcgcttaca cgataccgac cgttcaggtg
      301 cctgggcctt gctctttttt gttccgttta ttggctggct cgtgcttctg gtttttttct
      361 gcacagaagg tacttctggc agcaatcgtt acggaaacga tccgaagttt ggttcaaatt
      421 aatcttagaa ttggggcgat atttcgcccc tttttattaa caataataaa tattattttc
      481 cgctaaaaag tttgggaatt tcccgcagac accaggattt cgcttccccc atactgtcac
      541 gacgccaggc cataataata tcgatctcac tggtcgattc cgggctgacg acacgcaacc
      601 gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg ccaagccccg
      661 ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc tgtttgtcca
      721 gcagttgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca attccacgat
      781 atttcacgcg cgtcacttca gataacggtt ccggctcctg atgaatcggg tgatcaggcg
      841 cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc gacgaggaac
      901 gaaaatgcat atccggcgcg ataacaatat ccgcccgccc ctgctccagt cgttcctacg
      961 cccccgccag cacttcggtg atgattgcca gttgggtatt ggcttttgcc gccagtttgt
     1021 cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg gtgagatgcg
     1081 tttcccaacc gcgcgcgagg gcttccgcat cggtagtcag tttatctgcg gcttccagca
     1141 aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg cgatggcccg
     1201 agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatggtg tagctaagtg
     1261 cggaaggcac gcgtcccagc tcatccgccg ccgccgcaaa actgccccgg cgatcgcatc
     1321 cataacccgt agtgcttcca gcgttaatgc cctttctttg gccatttcgt tctcattcag
     1381 gaaatttgaa cataccgggc agaatatctg gctaacaatg tagcgtccag ccccttacca
     1441 taaaaggaag taaagagagg tcaagaatta tgattactac ccgaactgcc aggcagtgtg
     1501 gacaagcaga ctacggatgg ttgcaggccc ggtatacttt ttcctttgga cactacttcg
     1561 acccgaaatt gttaggctat gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag
     1621 gtgccgcctt tcagccgcga acctatccca aagtcgatat tttaaatgtg attctggatg
     1681 gagaagcaga gtatcgcgac agcgaaggca atcatgttca ggccagcgcc ggtgaggcgt
     1741 tgctgctctc tacccagccg ggtgtcagct acagcgaaca caatctcagc aaagacaaac
     1801 cgttaacacg aatgcagctt tggctggacg cctgcccgca gcgagagaat ccgctgattc
     1861 aaaaactggc gcttaatatg ggcaagcagc aattaatcgc ctcgccagag ggtacgatgg
     1921 gaagcctgca attacgccag caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa
     1981 gtgcgaattt ccagttgcat gggccacgcg cgtatttgca atcgattcac gggaaatttc
     2041 atgcgcttac gcatcatgaa gagaaagcgg cgctgacctg cggtgatggg gcgtttattc
     2101 gtgacgaggc taacattacg ctggttgccg attccccact gcgcgctttg ctgatagatt
     2161 tgcctgtcta gttgttttta cagggagatg atgatgagta aaaaatcggc caaaaagcgc
     2221 cagccagtga agcccgtggt ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat
     2281 gaagagatgt tgagcgagct ggaagccatc gtcgcggatg ctgaaacgcg tttagccgag
     2341 gatgaagcta ccgcgtaaag acaggttgcc ggatgcggcg aaaacgcctt atccggccta
     2401 caaaatccaa caacattaac gaattaaaca acccgtaggc cggataaggt gcgccagcat
     2461 cgcatccggc acaatactca aaacctggcg tttatctggc cttgctcgcc ataatctcga
     2521 taatctgccg atccgtttgc tgcatcgaat ggcttgctaa cgcacacagg ttggcaatcg
     2581 actgctcaac atcatgcgcc acgatccctt cattgccggt cacggcggta tcatccagcg
     2641 ccattaacac cgctttccac gcagccgaag cactggtcga aaccttcatc gcgcagctgt
     2701 tcgacgcacc atcgcaaatc atgccgctga catcgccgat catactgctg atcgccatcg
     2761 agatagtttc ataacgccca tccaccagcc atgccatccc ggcggcggcc cccattgctg
     2821 cggtcgttgc ggcacaaagt gcagacaaac gcggtaactg gttatggatg taaattgcgc
     2881 tcaaatgaga aagcatcagc gcacgcgcca gtcgttcatc atccgctccg aagtgttctg
     2941 ctacaaccac cacaggcatt gttgcggtga tcccctggtt acccgagccg gagttactca
     3001 tagccggaag cgtagcgccg cccatacgcg catcggatgc cgcgctggta cgaatcacaa
     3061 tggatgaaga gagatctttc gccagcaaac cgcgctcgca ctgtttttcc agcgtcgcgc
     3121 caatatgcag cccccactta ccgctcaaac cttcctgcga taacgcacaa tttagcttcg
     3181 cggaatcgag aataaagcgg atcgccgcaa acgggacttc attgacgaac ttcaggatct
     3241 cagccagcgt cgttctggaa agcactgtca gcggagactc ttgctcgccc tctgccacac
     3301 acgcctgctg ggtaaacacc acaccattgt gcgtctcaat atgcacaatg ttggtatgcc
     3361 cgccgacgat ggtgacacac gcccacttct caccgttcca gactttggcg cgtgagaaga
     3421 ggatttcatt gcaaggttcc tggatcttaa cggagacttt ccccgccgcc agcagtgctt
     3481 tggcatcggc aattgcctgc gcagttgcgt ctttcagcac ttccagcccg gcgttggcat
     3541 ttccacctaa cgcccccagc gccgccgcaa tcggcagccc caccattccc gtgccgggaa
     3601 cggtgacgcc cagaccgttc ttcatcagat ttggcgaaac ccaggcttct acacgttcaa
     3661 ccggaccttc cagttctgcc gcagcaaccg ccgccgccag cgccagtgaa atcggttcag
     3721 tacatcccag cgccggtttt acttcctcct gaacggcgag gatgtaacgc tgccataacg
     3781 gatttaaagt cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg
     3841 ccaggaacgg agaaacacaa agcaacaaac cagtgacgat aatcaggtac agagacatcc
     3901 ctttgtattt gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc aggcacccta
     3961 ccatgccgaa aatcgggcta cagatagagg tgaagctcaa caccggtgcg ttcagtacga
     4021 tagcgctcca ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt
     4081 taatcttctc ggcaggcatc ttgcgacgca ggatgttcat tacgatccct tgcgttgctt
     4141 cgcgaaagcc taaatagacg ccaaagaatg cagtcatgac agcaaagata ttgagaatga
     4201 cgctgaccac tttcacccac gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca
     4261 gcgcggaaat attctgctcg taggctttta ccgcttcgtc atgccccatc gccagcgtga
     4321 acgacacggc gtagaaaaag acggtgacaa acaaaatgcc aaacgcgata ttcattgccc
     4381 gcaatgcttt atgccgcgcc acttcaatag atttttcccg cgagcgataa gagatcacca
     4441 tcgggcttaa cgtctggata aacaaaatcg acgtcagggt aaacggcagc gtaataatgg
     4501 cgtttttcac cagcagcccc agcggcggta gcgaaccgac gttatacaga tgccacattc
     4561 ccaccatcga cacgcccagc gccgcgacca ccagcagctt ggtcagcacc atgccggtcg
     4621 aaattttgaa taacaatttc tcgccgcgtg aagagatcgc caccagaatg caaatcagta
     4681 ccagaccata aaagggactg tctgacagca acccttccgt cacgccgaag gtatgcagat
     4741 aggaagcact atcgttggtg atggcggtgg aatagacgaa catccaaatc accagcatta
     4801 cgaaatagaa cgcacctaac aggatgcccc agtttttacc taaataaccg ctaatgacac
     4861 tcgggtaatc tttacattct ggtgattctg ccagcgtatt aataaacaac cgctgaaaca
     4921 gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat cccatcaaac
     4981 cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata ctcataatca
     5041 cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc tcactcattc
     5101 cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat gcaatttcca
     5161 taatttctcg ctcaatattt tgtagggctt attatttttt tccgagccgc atcaaggcga
     5221 tatgcggtct acgtttttgc aggcgagtta ttagaataaa gaaatgatac gcgcgaggtc
     5281 agagaaaatc ttcacaatca agactttgaa ataacggaga tggataagaa ttttctacta
     5341 aattaatcgc aagagaatat tcattctcta tttgcggcag atcacaaaaa aaaggtgcac
     5401 atttgtgcac ccaaggatga aagctgacag caatgtcagc cgcagaccac tttaatggcc
     5461 agtcctccgc gtgatgtttc gcggtattta tcgttcatat ctttgccggt ttcatacatc
     5521 gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat cgccatccac
     5581 gcggcgttta ctgctttcac ggcattaatg gcattacgtt caatgcacgg gatttgtacc
     5641 tgtccggcaa ccggatcgca ggtcagccca aggttatgct ccatcgcgat ttccgccgca
     5701 ttgcatacct gcgccggact gccgcccagt agttcagtta accctgccgc cgccattgaa
     5761 caggccacgc caatctcccc ctgacagccg acctccgcgc cagagatgga ggcgttcatt
     5821 ttatacagcg cgccaatagc ccccgctgcc agaaaatagc gggcaattga ccgctcgttt
     5881 accggacgac ggaacttatc gtaataagcc agcactgccg gaataatgcc gcacgcaccg
     5941 ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt cttcactaac cgccagcgcg
     6001 tacatgttga tccagtcgat gacattcatc ggatcgttag agatgttatc gctagaaacc
     6061 agctgacgac gcagcgctac ggcacgacgc ggcacattga gcggaccagg cagcacgcct
     6121 tcagtgttca tcccgcgttc aataccgtca tgcatcactt gccagatacg ggcaaatcct
     6181 gcgtcaattt ccgctttgct gcgcagcgcc agctcgttgt gcatcatcag gccagatata
     6241 gacaggccgt tgtaatcaca cattttcagc agttcacctg ctgagtggaa atcgtaaggt
     6301 acggacgttt cgacatcgtg cgacaggccg aagtgttctt cttcgacaat aaacccgccg
     6361 ccgacagagt aataggtttt acttaatagc gcttcctgtc ccttccaggc agtgatccgc
     6421 attccgttct catggcgagg caacatttcg ggatggaaga taatgttctt tgctacagga
     6481 aaatcaacaa tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt tactaactct
     6541 ataaatgcag ggatctcatc aatgacaaca tcctgcggac tgtttcctgc cagtcccatg
     6601 ataatggcga catccgtggc atggcctttg cccgtcagtg acaacgaccc atacagatcg
     6661 accacaatat ggctcgtcgc ggttaataag ccgctacttt ccagccgatc aataaaactt
     6721 tttccggcat tcattggccc cacggtatgc gaactggagg gaccaatccc aattttgaaa
     6781 atatcgaatg cactaatcat atccacaccc tcggattgcc gttcagtgaa gtggagcgga
     6841 acgaccttgc gaccgtcccg ctcacgaggc tttacgcact acgtactgcg atagcttcaa
     6901 tttccagctt cacatctttc ggcaaacgcg caacctgcac acagctccgg gtcgggtagg
     6961 tcgcctgatg ctcatcgaag aactgcttat agacttcatt gatggtggca aaatcattca
     7021 gatcggtgat gaacacggtc atcttgatga tatcgcccac gctcagcccg gcagcaacca
     7081 cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc ttgcacatca gccgggatct
     7141 caccggtctg cgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg cctaaatcaa
     7201 cgccctgaac ataagggccg attgcgcctg gggcacgttg cgtttcgata atctttttca
     7261 tacatcctcc ggcgtcagag cgcctgggta aaagtacgtg aaataacatc ctgttgctgt
     7321 tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga cacggattgt caggttagga
     7381 tatttttccg ggtgctcgat ggcatccagc agcatttccc gattcattac gttgacgttg
     7441 aggtgttgac cgccttcgac atccgcttcg tggtggaaat acccatccag caggccgaca
     7501 aggttggttt tacgtactgg atcttctttg cccagcgccg caggaacgat tgagaaggtg
     7561 tacgagatcc catctttggc gtaggtgaac ggcagtttcg ccaccgacgt caatgaggcc
     7621 acggcacctt tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa cggtgttccg
     7681 gcgcgacgac cgtccggcgt attaccggtt ttctggccgt acaccacgtt agaagtgata
     7741 gtcagaatcg actgggtagg gacggcgttg cgataggttg gcagcgcttt aattttcttc
     7801 ataaagcgtt caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt gttgccgtac
     7861 tgcggatatt caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc acggattggt
     7921 ttcacgcggg catatttgat ggcagacagg gagtccgtcg ccaccgacag gcccgcgatg
     7981 ccgcatgcca tagtgcgata gacatcacga tcgtgcagcg ccatcagcga agcttcgtag
     8041 ctgtacttgt cgtgcatgta gtgaatgata ttcagcgcgc tgatgtactg caccgccagc
     8101 cagtccatga agtgatcgag gctgtccatc actttgtcgt aatccagcac gtcgtccatc
     8161 agcggtgctg ttttcggccc gacctgaatc ttcagcttct cgtccacccc gccgttaatt
     8221 gcgtacagca gtgttttcgc caggttagcg cgtgcaccaa agaactgcat ttgcttacca
     8281 atcaccattg ggctgacgca acaagcaatt gcgtaatcgt cgctgttgaa gtcagtacgc
     8341 atcagatcgt cgttttcata ctgcaaggaa gaggtgacga tcgacacctg cgcggcatat
     8401 tttttgaagg caatcggtaa ttcttccgac caaagaatgg tcaggttagg ttccggtgcc
     8461 ggccccatag tgtgcagggt gtgcaaatag cggaaggagt ttttggtcac cagcgtacga
     8521 ccgtccagcc ccatcccgcc gatcacttcc gtcgcccaga ttgggtcgcc ggagaacagc
     8581 gaatcaaatt ccggcgtacg caagaagcgc accatacgga tcttcataat gaagtgatcg
     8641 atcagttcct gtgcctgctg ctcattgagt acgcctgctt taaagtcgcg ctcaatgtag
     8701 atatcgagga acgacgccgt gcgccccagc gacatcgcgc cgccgttctg tgatttcact
     8761 gccgccagat aagcgaagta gagccactgc accgcttcct gcgcattctg cgccgggcga
     8821 gagatatcaa agccatattt cgccgccatt tcctgaatct gcaacagcgc atgacgatgc
     8881 tctgccagct cctcacgcag acggatggtg gcttccagat cttcgccttt ttccagacgg
     8941 gactggagat cggcaaattg cagttcgcgt tcacgcacca gataactgat gccatacagc
     9001 gctacgcggc gatagtcacc gataatgcgc ccacggccat agccatctgg taaaccggtc
     9061 agcacgccag atttacggca gcgcagcata tccggtgagt aaacatcaaa tacgccctgg
     9121 ttatgggttt tacgcagatc ggtaaacaga tattcaaatt cactgtccat ttcacggcca
     9181 taggcgtgga atgaactttt aatcatatta atgccaccga acggatgcag cgcgcgcttt
     9241 aatggcgcat ccgtttgcag accaacaatt ttttccagcg gctggttaat atatcccgca
     9301 tcatgagcgg taattgtggt ggcaatattg gtatcgaaat caaccggcgc gtgggttgcg
     9361 ttttcgatac ggatgccttc cattactttt tcccacaatt ccgtggtggc aggcgtcgct
     9421 tcggcgagga aagattcatc gccttcatac ggtgtatagt tatgttgaat aaaatcgcgg
     9481 acattaattt cgtttttcca gtccgtacct ttaaagccaa gccatgcgtc ggcgtacagc
     9541 ttatcgctgg tatcaatatc tatcttcatg aaaaataatc tctctacaat acttcaacta
     9601 aattatgcaa attctgcggg cgcgttaact ttgcctaaat gaatggcatc caaagcaatc
     9661 attttttctt cgttagtcgg aataacggca caaatgacac gcgcattttc actggaaaca
     9721 attcgctctc caaaagagtt agagcgatta ttcatttctg tatcaatcac tacgcctaat
     9781 acagccaaat gttccatgac cagacgacga attaaacttg aattctctcc tattccgccg
     9841 gtgaatataa ttccatccag gcgacgtaat gaagctgcgt gtccggcaat atgacgggca
     9901 attcggtgaa caaaggtttt aattgccagt tgcgcgcgtt cgtgaccttc atgccaggct
     9961 ttttccagaa cacgtaaatc cgaagaaaga ccggaaatac ctaataatcc cgactcttta
    10021 ttcactacgc gttccaggtc acccaggctc tggttggttt ggctggcgac ccaggacatc
    10081 gcaccaaagt cgacatcgcc actgcgggtt cccatcatca agccttccag cggcgtcatt
    10141 cccattgaag tatcaacact ctgaccgttg cgaaccgcgc agattgacgc gccattgcca
    10201 agatgcgcca caaccaggcc ggagtcatct tccgccagat tcagcagaga atgtgcgcgc
    10261 tgggaaacat agcggtgcga cgtgccgtgg aaaccataac ggcgtacacc taactcttca
    10321 taatatttcc acggcaggcc gtataaataa gcttccggag ccatcgtctg gtggaaactg
    10381 gtatcaaata ccgccacctg agttacgccc ggaaataatt gctgcgccga ttcaatacca
    10441 cttaaattgg cgtaattatg caggggtgcc agtggagaaa cgcgacggat attatcaatg
    10501 acttcatcgg taataatggc ggactcggta aaaatactgc cgccgtgagc gatgcggtgg
    10561 ccaattaagg ccacactgtc atttaaattc cgtttttcca gttcaaatgc aattgccttc
    10621 aatgcacctt cgtagctgtg gtgagccagc ggtgctggct ctcccccatt tacggataag
    10681 aatgcatttt ccgagttaat accgtcggca atacctgaca ttaatacttc acagtcgctg
    10741 gcatcgagca cggaaaactt aatcgaagac gaaccacagt taataaccaa aacaaccgga
    10801 aattcattca tctcttttct catcctgagt tacggattaa aacagtttgt atacgatgtt
    10861 caggatggtc agcagaccaa tcacggtaac aaacacgtta tccagacgac cacggtattt
    10921 cgccagagac ggcgctttac ggatggcata catcggcaac aggcacagca gggatgcgat
    10981 aatcggtgcg cccatggctt caatcaggtc gaggatgttc gggttggcgt aggcaacaac
    11041 ccaggtggag cccatgatga agatcatgct gatagtgttc agtttaccca gcgacacttt
    11101 agttttgtcg cctttataac caaacttcag gaccaggcca ttcagacctt ccagcgttcc
    11161 cagatagtga ccgaagaaag atttgaagat agccacgagt gcgatgatgg aagccgcata
    11221 ttccagtgta atcgcgaacg ttgttttggt accggtcatg gacgcaaagt ggttagccag
    11281 ataagaaagc actggaatat tctgcgcttt ggcttccgcc atgttggccg gagacagcgt
    11341 aaataggcag ctaaaggcaa agaacatcac cactgcaacc atcagcatgc tggcacgaga
    11401 aatgatttgg gaacatttac gttcggtgaa gtcgcgaccg aagtctttct catactcttc
    11461 acgtttagaa accacgaagg aagagacgat tggcgagaaa ttaaaggaga aaaccatgat
    11521 ggaaatcccc agccagacgg tgatcaggat accgtcatga ccggttaacg acagcgaacc
    11581 gaggtcaacc tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca gagaaatcag
    11641 caccaggctg gcgataaacg gccataccag gtagctcatc actttaacca tcagatcctt
    11701 accaaaccag atgacgaaag ccatcagcag cagcaggaac agcgccacaa agccgcgatt
    11761 cagcggtgca aagccgagct ggttttccca gaacgtcata aaggtattgg taatagtaac
    11821 gccataaatc cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga taaccacgcc
    11881 gccagtttta ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag ggttagagcc
    11941 agaaagacat agacgcgcca gcgcccggtg gcaataaaat gcgatggggt atgccaatac
    12001 caacatcaga agaatcggga tcagtccgcc aaaacctgcg cggataggga agaacagcac
    12061 cccggcgccg attgccgtac caaacaagcc taacgtccat gtggtatctg atttacgcca
    12121 ggacgattgt tttgtctggc tggatacaat gctatctgaa gtactcatat cctatcctca
    12181 acgaattaat taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa gatcgatatt
    12241 gccgccggaa ataatactga cggtttttct gttttgaata tattggtcta atttaccgct
    12301 taataatgca gcacatgcca gagcgcctgc gccttcggtg acgactttat tgcgctgaat
    12361 taaggcaatc atactgtttc tgatttcgtc ttcgctgacc agcacgatgt catcgactaa
    12421 ttcacgaacg atttcgtaag ttaaattacc cgggcgggag acatcacaac catccgccag
    12481 ggtgccggta gttcggtgcg tggttatttc tccggagtgg aaagaagccg ccatgccgtg
    12541 aacgttttca gactgtacgc caataacacg aatggtcggg ttaatagatt taattgccac
    12601 cgcaatacca gcaattaaac cgccaccacc aattggcaca atcacgttat cgacatcata
    12661 gagatcttcc ataatttcca gaccaatcgt tccctggcca gcaatcactt tcggatcatc
    12721 gtaaggtggg ataaaaatac ggccttccat ttcgacaatt tcgctcactt tagcgatagt
    12781 gtcgttgaag ttatcaccat gcagaacgac ttctgcggag tagtcgcacg ttgccgctac
    12841 tttggatttt ggcgcacctt ttggcatcac cactttaccg tcgataccca gcatcgcgca
    12901 ggagagcgaa accccttgcg catggttgcc cgcagaacag gccaccacgc ctttgcgttt
    12961 ttccgcatcg gtcagtgaac ttaatttatt aaatgcgcca cgaattttaa atgaacccgt
    13021 acgctgcata ttttcaaact tcaggaatat ttcacctttg caacgttcac taaaatagtt
    13081 ggagcgaggc atgcctgttt tataaattcg cccagccagt cgttgtttcg cttcaataat
    13141 atcatcaata gcaatcggca gatcgtatgt aatatgcatt ataaaacctc ttcgcctgaa
    13201 ttaaatatgt aggtaaccgt aaccgacacc t
//
LOCUS       DAZPBM010000114        13210 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPBM010000114 DAZPBM010000000
VERSION     DAZPBM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.114"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHUZQ_002948"
     CDS             <1..807
                     /locus_tag="ACHUZQ_002948"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR1026588.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1177..2001
                     /gene="gadX"
                     /locus_tag="ACHUZQ_002949"
     CDS             1177..2001
                     /gene="gadX"
                     /locus_tag="ACHUZQ_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1026589.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            2369..3097
                     /gene="gadW"
                     /locus_tag="ACHUZQ_002950"
     CDS             2369..3097
                     /gene="gadW"
                     /locus_tag="ACHUZQ_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1026590.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3460..6573)
                     /gene="mdtF"
                     /locus_tag="ACHUZQ_002951"
     CDS             complement(3460..6573)
                     /gene="mdtF"
                     /locus_tag="ACHUZQ_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR1026591.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(6598..7707)
                     /gene="mdtE"
                     /locus_tag="ACHUZQ_002952"
     CDS             complement(6598..7707)
                     /gene="mdtE"
                     /locus_tag="ACHUZQ_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR1026592.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7815..8093
                     /locus_tag="ACHUZQ_002953"
     CDS             7815..8093
                     /locus_tag="ACHUZQ_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026593.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="ACHUZQ_002954"
     CDS             complement(8094..8621)
                     /gene="gadE"
                     /locus_tag="ACHUZQ_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1026594.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="ACHUZQ_002955"
     CDS             complement(9420..9992)
                     /gene="hdeD"
                     /locus_tag="ACHUZQ_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1026595.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            10247..10579
                     /gene="hdeA"
                     /locus_tag="ACHUZQ_002956"
     CDS             10247..10579
                     /gene="hdeA"
                     /locus_tag="ACHUZQ_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1026596.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10695..11021
                     /gene="hdeB"
                     /locus_tag="ACHUZQ_002957"
     CDS             10695..11021
                     /gene="hdeB"
                     /locus_tag="ACHUZQ_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1026597.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            11085..11732
                     /locus_tag="ACHUZQ_002958"
     CDS             11085..11732
                     /locus_tag="ACHUZQ_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR1026598.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(11774..12304)
                     /locus_tag="ACHUZQ_002959"
     CDS             complement(11774..12304)
                     /locus_tag="ACHUZQ_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1026599.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(12460..13026)
                     /gene="slp"
                     /locus_tag="ACHUZQ_002960"
     CDS             complement(12460..13026)
                     /gene="slp"
                     /locus_tag="ACHUZQ_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1026600.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3599 a         2983 c         3030 g         3598 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca cacctgataa cataacgttg taaaaaccga atgcccagcc
      841 tttaaaaaaa cagctgggca ttcggttgct tattaatcgc aataatatat tggctgtcta
      901 ttcatcgtgt tgataagata tatgttatat gaatgtttat taaatgtagc gattgccttt
      961 aatgtcacga aaagggatta tttgcttgct attaatttca ctgcgttctt aaacacattt
     1021 ttgtattata aatccagtca tcctgcccgt agaacatcac acattatcat cctgttctcc
     1081 cgcttcgttt aaatttattt atcaatcaat ttgacttaag agggcggcgt gctacattaa
     1141 ttaacagtaa tatgtttatg taatattaag tcaactatgc aatcattaca tgggaattgt
     1201 ctaattgcgt acgcaagaca taaatatatt ctcaccatgg ttaatggtga atatcgctat
     1261 tttaatggcg gtgacctggt ttttgcggat gcaagccaaa ttcgagtaga taagtgtgtt
     1321 gaaaattttg tattagtatc aagggatacg ctttcattat ttctgccgat gctcaaggag
     1381 gaggcattaa atcttcatgc acataaaaaa atttcttcat tactcgttca tcactgtagc
     1441 agagatattc ctgtttttca ggaagttgcg caactatcgc agaataagaa tcttcgctat
     1501 gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt tatctgtttt tcttgaggat
     1561 gagcacttta taccgctgct tttgaacgtt ttgcaaccga acatgcgaac acgagtttgt
     1621 acggttatca ataataatat cgcccatgag tggacactag cccgaatcgc cagcgagctg
     1681 ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag aagagacatc atattcacag
     1741 ttgcttactg agtgtagaat gcaacgtgct ttgcaactta ttgttataca tggcttttca
     1801 attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt cgtatttcat ttacgtcttt
     1861 cgaaattatt atgggatgac gcccacagag tatcaggagc gatcggcgca gggattgccg
     1921 aaccgtgact cggcggcaag tattgttgcg caaggaaatt tttacggcac tgaccgttct
     1981 gcggaaggaa taagattata gaattttact cagacataaa aaaaacccgg cataggggac
     2041 cgggaagagg atagtctgcc gtctccagac taataaaccg ttataacact ccctgttggc
     2101 acgggaaact ttgtgctctc agtaagttaa atataacttt tactggaaat aagatcagcc
     2161 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
     2221 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
     2281 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
     2341 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
     2401 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
     2461 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
     2521 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
     2581 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
     2641 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
     2701 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
     2761 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
     2821 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
     2881 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
     2941 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
     3001 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
     3061 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
     3121 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     3181 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     3241 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     3301 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     3361 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     3421 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     3481 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     3541 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     3601 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     3661 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     3721 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     3781 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     3841 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     3901 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     3961 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     4021 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     4081 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     4141 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     4201 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     4261 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     4321 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     4381 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     4441 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     4501 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     4561 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     4621 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     4681 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     4741 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     4801 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     4861 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     4921 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     4981 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     5041 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     5101 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     5161 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     5221 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     5281 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     5341 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     5401 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     5461 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     5521 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     5581 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     5641 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     5701 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     5761 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     5821 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     5881 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     5941 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     6001 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     6061 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     6121 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     6181 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     6241 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     6301 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     6361 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     6421 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     6481 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     6541 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     6601 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     6661 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     6721 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     6781 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     6841 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     6901 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     6961 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     7021 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     7081 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     7141 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     7201 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     7261 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     7321 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     7381 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     7441 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     7501 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     7561 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     7621 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     7681 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     7741 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttc tttaaattgc
     7801 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     7861 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     7921 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     7981 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     8041 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     8101 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     8161 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     8221 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     8281 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     8341 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     8401 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     8461 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     8521 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     8581 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     8641 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     8701 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     8761 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     8821 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     8881 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     8941 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     9001 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     9061 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     9121 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     9181 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     9241 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     9301 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     9361 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     9421 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     9481 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     9541 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     9601 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     9661 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     9721 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     9781 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     9841 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     9901 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     9961 ttcaaaattg ttgccttatc tatatataac atagaaccac cctataaaat taagaagaaa
    10021 atcccctgct atcaatctat gccaaaaacg cgtctaagaa tgtagtcgat ttaataaaaa
    10081 tttcctaatt acagtatctg atgcatctgt aactcattgt attgaaataa aaatatctga
    10141 ttttgatatt ttatatcaac atgacatata cagaaaacca ggttataacc tcagtgtcga
    10201 aattgattcg tgacggctct ttcactttat agttgaggat attacgatga aaaaagtatt
    10261 aggcgttatt cttggtggtc tgcttcttct gccagttgtg agcaatgcag cggatgcgca
    10321 aaaagcagct gataacaaaa aaccggtcaa ctcctggacc tgtgaagatt tcctggctgt
    10381 ggacgaatcc ttccagccaa ctgcagttgg ttttgctgaa gcgctgaaca acaaagataa
    10441 accagaagat gcggttttag atgttcaggg tattgcaacc gtaaccccag ctatcgttca
    10501 ggcttgtact caggataaac aagccaactt taaagataaa gttaaaggcg aatgggacaa
    10561 aattaagaaa gatatgtaat tccgggaatg cgttacatcg tacttccttg catattgaac
    10621 aggccggaat atcttcttta aaagcagcta ttcctcctgt tcatatataa tctctatatt
    10681 gaatgggtta caaaatgaat atttcatctc tccgtaaagc gtttattttt atgggcgctg
    10741 tagcggcttt gtcactggtg aacgcacaat ctgcgctggc agccaatgaa tccgctaaag
    10801 atatgacctg ccaggaattt attgatctga atccaaaagc aatgaccccg gttgcatggt
    10861 ggatgctgca tgaagaaaca gtatataaag gtggcgatac cgttacttta aatgaaaccg
    10921 atctcactca aattcctaaa gtgatcgaat actgtaagaa aaacccgcag aaaaatttgt
    10981 ataccttcaa aaatcaagca tctaatgact tgccgaatta atgaggtaca agtaaaaagg
    11041 agtagcaagt tgagccatct tgctgctcct ttttgcattt ttatatgaca gcagaattta
    11101 ttatacgtct tatactcgcg gcaattgcct gtggcgctat tggcatggaa aggcaaatgc
    11161 gcggcaaagg agcagggtta cgcacacatg tattaattgg catgggaagc gccctgttta
    11221 tgattgtttc gaaatatggt tttgctgacg tgctgtcttt agatcacgtc ggactcgacc
    11281 ccagccgtat tgctgctcag gtggtgacgg gcgtcgggtt tatcggtgca ggtaacattc
    11341 tcgttcgtaa ccaaaatatt gtaggtctga cgacggcagc ggatatctgg gtgaccgccg
    11401 ccataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
    11461 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
    11521 atttacagct gacattagtg aacggtaata tggtatcaat gctcgactgg tttaagcagc
    11581 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
    11641 ttgatatcca gcttcatgcc acaacgtcga tagaagacct gttgcgatta ttaaaaggta
    11701 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
    11761 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
    11821 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
    11881 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
    11941 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
    12001 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
    12061 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
    12121 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
    12181 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
    12241 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
    12301 acataaccga ctcctggtgc ggacattaac tttataatca atttgccaaa ggtaattaac
    12361 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
    12421 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
    12481 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
    12541 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
    12601 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagccagg
    12661 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactgggtc
    12721 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggttt
    12781 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
    12841 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
    12901 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
    12961 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
    13021 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
    13081 ttcatctgat tctgaggcga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
    13141 ccttttgttt agttcaattc cattgatttt ggggcgtggc atgagtatcc atcgccccat
    13201 aatataatcc
//
LOCUS       DAZPBM010000115        13076 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPBM010000115 DAZPBM010000000
VERSION     DAZPBM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.115"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            270..605
                     /locus_tag="ACHUZQ_002961"
     CDS             270..605
                     /locus_tag="ACHUZQ_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1026601.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            763..1206
                     /gene="yjdN"
                     /locus_tag="ACHUZQ_002962"
                     /pseudo
     CDS             763..1206
                     /gene="yjdN"
                     /locus_tag="ACHUZQ_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            1339..2127
                     /gene="phnC"
                     /locus_tag="ACHUZQ_002963"
     CDS             1339..2127
                     /gene="phnC"
                     /locus_tag="ACHUZQ_002963"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1026602.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2152..3168
                     /gene="phnD"
                     /locus_tag="ACHUZQ_002964"
     CDS             2152..3168
                     /gene="phnD"
                     /locus_tag="ACHUZQ_002964"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1026603.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3294..4073
                     /gene="phnE"
                     /locus_tag="ACHUZQ_002965"
     CDS             3294..4073
                     /gene="phnE"
                     /locus_tag="ACHUZQ_002965"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR1026604.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4094..4819
                     /gene="phnF"
                     /locus_tag="ACHUZQ_002966"
     CDS             4094..4819
                     /gene="phnF"
                     /locus_tag="ACHUZQ_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR1026605.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4820..5272
                     /gene="phnG"
                     /locus_tag="ACHUZQ_002967"
     CDS             4820..5272
                     /gene="phnG"
                     /locus_tag="ACHUZQ_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR1026606.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5269..5853
                     /gene="phnH"
                     /locus_tag="ACHUZQ_002968"
     CDS             5269..5853
                     /gene="phnH"
                     /locus_tag="ACHUZQ_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR1026607.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5853..6917
                     /gene="phnI"
                     /locus_tag="ACHUZQ_002969"
     CDS             5853..6917
                     /gene="phnI"
                     /locus_tag="ACHUZQ_002969"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR1026608.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6910..7755
                     /gene="phnJ"
                     /locus_tag="ACHUZQ_002970"
     CDS             6910..7755
                     /gene="phnJ"
                     /locus_tag="ACHUZQ_002970"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR1026609.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7752..8510
                     /gene="phnK"
                     /locus_tag="ACHUZQ_002971"
     CDS             7752..8510
                     /gene="phnK"
                     /locus_tag="ACHUZQ_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR1026610.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8621..9301
                     /gene="phnL"
                     /locus_tag="ACHUZQ_002972"
     CDS             8621..9301
                     /gene="phnL"
                     /locus_tag="ACHUZQ_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR1026611.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            9298..10433
                     /gene="phnM"
                     /locus_tag="ACHUZQ_002973"
                     /pseudo
     CDS             9298..10433
                     /gene="phnM"
                     /locus_tag="ACHUZQ_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
     gene            10433..10990
                     /gene="phnN"
                     /locus_tag="ACHUZQ_002974"
     CDS             10433..10990
                     /gene="phnN"
                     /locus_tag="ACHUZQ_002974"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR1026612.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10977..11411
                     /gene="phnO"
                     /locus_tag="ACHUZQ_002975"
     CDS             10977..11411
                     /gene="phnO"
                     /locus_tag="ACHUZQ_002975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR1026613.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11413..12171
                     /gene="phnP"
                     /locus_tag="ACHUZQ_002976"
     CDS             11413..12171
                     /gene="phnP"
                     /locus_tag="ACHUZQ_002976"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR1026614.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12318..12647)
                     /gene="yjdP"
                     /locus_tag="ACHUZQ_002977"
     CDS             complement(12318..12647)
                     /gene="yjdP"
                     /locus_tag="ACHUZQ_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR1026615.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12716..13033)
                     /locus_tag="ACHUZQ_002978"
     CDS             complement(12716..13033)
                     /locus_tag="ACHUZQ_002978"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR1026616.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2878 a         3915 c         3674 g         2609 t
ORIGIN      
        1 taactcttct gatgcgttta aaacgtcaga aagataaaaa tatcatgtga ataaaaaaaa
       61 gaacaagtag agcattaaca ttatcttaaa taataaatag aggcaaaaag attattttct
      121 ttttgcgttt cctttcaaat gaaaacgatc gtcgtctaaa atcagcagta cccccgacaa
      181 actcagggat tttgtgtata attgcggcct ttttcggcaa tctgccgttt tttggcgctt
      241 ttgccctgct gacttttgag gaaatccaca tgtcattacc acactgccca aaatgcaact
      301 ccgaatacac ttacgaagat aacggcatgt acatctgccc ggaatgtgcc tacgaatgga
      361 acgacgcaga acctgcacag gaaagcgacg agctgatcgt taaagatgct aacggcaatc
      421 tgctggctga cggcgacagc gttaccatca ttaaagatct gaaggtgaaa ggtagctctt
      481 cgatgctgaa aattggcacc aaagtgaaaa acatccgcct ggttgaaggc gaccataaca
      541 tcgattgcaa aatcgacggt tttggtccga tgaaactgaa atctgagttt gtgaaaaaga
      601 actgattgta ttgtgatcgg taagccggat aaggcgctcg cgccgcatcc ggcaacgatg
      661 ccgactgcct gatgcgacgc ttgcgcgtct tatcaggcag cctctcatct gtataaattt
      721 cgaactacac ttaattggct tctcttaact gaggtcacca tcatgccgtt aagtccctac
      781 ctctcttttg ccggtaactg tgccgacgca attgcttatt atcaacgtac gttgggcgct
      841 gaactgctct ataaaatcag cttcggcgaa atgccaaaat cagcgcagga cagcgccgag
      901 aactgccctt ccggaatgca atttcccgat accgccatcg cccacgccaa tgtgcgcatt
      961 gccggaagcg acatcatgat gagcgatgcc atgccgtcag gaaaagccag ctactccggc
     1021 tttacgctgg tgctcgattc gcaacaggtc gaagaaggaa aacgctggtt tgacaatctt
     1081 gccgctaacg gaaaaatcga aatggcctgg caggaaactt tctaggcgca tggctttggc
     1141 aaagtcaccg ataaatttgg cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg
     1201 caataacccg ccgggaggcc cgccctcccg cactgtcatc gaattcccgt taactcttca
     1261 tctgttagtc acttttaatt aaccaaatcg tcacaataat ccgccacgat ggagccactt
     1321 ttttagggag gctgcatcat gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc
     1381 aatcagcatc aggcgctgca tgcggttgat ctgaacattc atcacggtga aatggtggct
     1441 ctgcttgggc cgtcgggttc cggcaaatcc acccttttac gtcacttaag cggtttgatt
     1501 accggcgata aatccgccgg cagccatatc gagctgctgg gccgcacagt ccagcgcgaa
     1561 ggccgtctgg cgcgcgatat ccgcaaaagc cgcgccaaca ccggctacat cttccaacaa
     1621 ttcaacctgg tgaaccgcct gagcgtactg gagaacgtgc tgattggcgc actcggcagc
     1681 acgccgttct ggcgcacctg ttttagctgg tttacccgcg agcagaaaca acgcgcgtta
     1741 caggcgctga cccgcgttgg catggtgcat tttgcccatc aacgcgtttc caccctctcc
     1801 ggcggacagc agcagcgtgt ggcgattgcc cgcgcgctga tgcagcaggc gaaggtgatt
     1861 ctggccgatg aacccatcgc ctcgctggac ccggaatccg ccggcatcgt gatggacacc
     1921 ctgcgcgaca tcaatcagaa cgacggcatc accgtggtcg tcacgctgca tcaggtggat
     1981 tacgccctgc gctactgcga acgcatcgtc gccctgcgcc aggggcacgt tttctacgac
     2041 ggcagcagcc aacagtttga taacgaacgt tttgaccatc tctaccgcag cattaatcgc
     2101 atcgaagaga acgcgaaagc tgcctgacat ccccatcatt gaggaaaacg aatgaacgct
     2161 aagataattg cctcgctggc cttcaccagc atgttcagcc tcagcaccct gttaagcccg
     2221 gcacacgccg aagagcagga aaaggcgctg aatttcggca ttatttcaac ggaatcacag
     2281 caaaacctga aaccgcaatg gacgccattc ttacaggata tggagaagaa gctgggcgtg
     2341 aaggtgaacg ccttctttgc cccagactac gcaggcatta tccagggaat gcgcttcaat
     2401 aaagtggata tcgcctggta cggcaacctg tcggcaatgg aagcggtgga tcgcgccaac
     2461 ggccaggtct tcgcccagac ggtcgcggcg gatggatcgc caggttactg gagcgtgttg
     2521 atcgtcaaca aagatagtcc gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat
     2581 ctcaccttcg gcaatggcga tcctaactcc acctctggct tcctcgtccc cggttactac
     2641 gtctttgcca aaaacaatat ctccgccagc gactttaagc gcaccgtcaa cgccgggcat
     2701 gaaaccaacg cgctggccgt cgccaacaag caggtggatg ttgccaccaa caacaccgaa
     2761 aacctcgaca agctgaaaac ctccgcgcca gagaagctga aagaactgaa ggtgatctgg
     2821 aagtcgccgc tgatcccagg cgatccgatc gtctggcgca agaatctctc cgaaaccacc
     2881 aaagacaaga tctacgactt ctttatgaac tacggaaaaa cgccggaaga gaaagcggtg
     2941 ctggaacgcc tgggctgggc accgtttcgc gcctccagcg acctgcaact ggtgccgatt
     3001 cgccagctcg cactgtttaa agagattcag ggcgtaaaaa gcaataacgg actgaatgag
     3061 caggacaagc tggcgaaaac caccgagatt caggcgcagc tggatgacct ggaccgcctg
     3121 aacaacgcgt taagcgcgat gagttcggtg agtaaagcgg tgcagtaatg tgctcgatcg
     3181 gtcccctctc ccttgaggga gagggttagg gtgaggggga aatgcgcacg ctgatgccct
     3241 caccccggcc ctctcccaca gggagaggga gaaaacatta aacggagcca aacatgcaaa
     3301 ccatcaccat cgccccaccc aagcgcagct ggttctcgct tctgagctgg gccattgttc
     3361 tcgccgtgtt ggtcgtctcg tggcagggcg cggaaatggc cccgcttacg ctgatcaaag
     3421 acggcggcaa catggcgacc ttcgccgccg acttcttccc gcccgatttc agccagtggc
     3481 aggattacct caccgaaatg gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg
     3541 cagtggttct ctccatcccc ttcggcctga tgagcgccga aaacctggtg ccgtggtggg
     3601 tttaccagcc cgttcgccgc ctgatggacg cctgccgcgc cattaacgaa atggtcttcg
     3661 ccatgctgtt cgtggtcgcc gtcggtctcg gaccgttcgc tggcgtgctg gcgctattta
     3721 ttcacaccac cggcgtgctc tcgaaactgc tttccgaagc ggtggaagcg attgaacccg
     3781 gcccggtgga aggcattcgc gccaccggcg ccaacaagct ggaagagatc ctttacggcg
     3841 tgctgccaca ggtaatgccg ctgctgatct cctactccct ctatcgcttc gaatccaacg
     3901 tccgctcggc gaccgtcgtc ggcatggtcg gtgcaggcgg gatcggcgtc accctgtggg
     3961 aagcgattcg cggtttccag ttccaacaaa cctgcgccct gatggtgctt atcatcgtca
     4021 cggtcagcct gctggatttc ctctctcaac ggttgcgtaa gcactttatc tgataagtga
     4081 ggcattgata tctatgcact tgtctacaca tccgaccagc tacccaacac gctatcagga
     4141 gatagccgca aaacttgagc aggagcttcg tcaacactat cgctgcggcg actatcttcc
     4201 cgccgagcag caactggcgg cgcgctttga ggtaaaccgc cacaccctgc gccgcgccat
     4261 cgaccaactg gtggaaaaag gctgggtaca gcgccgtcag ggcatcggcg tgctggtgct
     4321 gatgcgcccg ttcgattacc cgctcaacgc ccaggcgcgt tttagccaga acctgctgga
     4381 ccagggcagc catcccacca gcgaaaaact gctgtcggta ttgcgccccg cgtccggtca
     4441 cgttgctgac gcgctgggga ttaccgaggg tgagaacgtt atccatttgc gcaccctgcg
     4501 tcgcgtcaac ggcgtcgcgc tctgtttaat cgaccactac tttgcggacc tcaccctctg
     4561 gccgacactg caacgtttcg acagcggttc gctgcacgat tttctgcgcg agcaaaccgg
     4621 aattgcgctg cgccgcagcc agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg
     4681 ccagcgcctt gaaatcccga atatgtcgcc gctgctgtgc gtgcgcaccc ttaaccaccg
     4741 tgacggtgaa agcagcccgg cggagtactc cgtcagcctg acgcgcgccg acatgattga
     4801 attcactatg gagcactgaa tgcacgcaga taccgcgacc cgccagcact ggatgtccgt
     4861 actggcgcac agccaaccgg ctgaactggc agcccgcctg aacgcgctga acatcaccgc
     4921 cgactatgaa gtgatccgca ctgccgaaac tggcctggtg cagattcagg cgcgaatggg
     4981 cggtaccggc gaacgctttt ttgccggcga cgccacgctg acccgtgccg ccgtacgcct
     5041 gactgacggc accctcggct acagctgggt gctggggcgt gataaacagc acgccgaacg
     5101 ctgcgcgctg attgatgcgc tgatgcagca atctcgccac tttcaaaact tatcagaaac
     5161 ccttattgcc ccgctggacg ctgaccgtat ggcacgcatt gccgcacgcc aggccgaagt
     5221 gaacgccagc cgggtcgact tctttacgat ggttcgcgga gacaacgcat gaccctggaa
     5281 accgctttta tgcttcccgt gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc
     5341 atgagcgagc cgggcgtgat tgtcgccctg tatcagctca aacgcggctg gcagccgctg
     5401 aatatcgcca ccaccagcgt gctgctgacg ctggccgata acgacacgcc ggtgtggctt
     5461 gccacgccat taagtaacga tatcgtcaat cagagcctgc gttttcatac caacgcgccg
     5521 ctggtcagcc agccggaaca ggcgaccttc gcggtgacgg atgaggcgat ttccagcgaa
     5581 cagctcaacg ccctttccac cgacaccgcc gttgcgccgg aagcgggcgc gacgctgatt
     5641 ttacaggtcg ccagcctgag cggcggacgc atgttgcgtc tcaccggcgc gggcattgcc
     5701 gaagaacgaa tgatcgctcc gcagctgcca gagtgcattc tgcacgaact caccgagcgc
     5761 ccgcacccgt tcccgctcgg catcgaccta atcctgacct gcggcgagcg cctgctggct
     5821 atcccgcgaa ccactcatgt ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaaa
     5881 aggcgatcga cgccgcccac gccctgcaag agagccgacg ccggggcgat accgatttgc
     5941 ccgaactgag cgtcgcccag attgaacagc agcttaacct cgcggtggat cgcgtgatga
     6001 cagaaggcgg cattgccgac cgcgaactgg cggcgctggc gctgaaacag gccagcggcg
     6061 ataacgtcga agcgattttc ctgctacgcg cctaccgcac cacgctgacg aaactggcgg
     6121 taagcgagcc gctcgacacc acggagatgc gtctcgaacg gcgtatttca gcggtttata
     6181 aagatattcc cggcggccag ctgcttggcc caacctacga ctacacccat cgcctgctcg
     6241 atttcactct gctggcaaac ggcgaagcgc cgacgctgac caccgccgac agcgaacaac
     6301 agccgtcgcc gcacgttttc agcctgctgg cgcgtcaggg gctggcgaag tttgaagagg
     6361 atagcggagc acagccggat gacatcaccc gcacgccgcc ggtttacccc tgttcacgtt
     6421 cttcccgttt gcagcagtta atgcgcggcg acgaaggcta tttgctggcg ctggcttact
     6481 ccacccagcg cggttacggg cgcaaccacc cgttcgcggg cgagatccgc agtggttaca
     6541 tcgacgtgtc gattgtgccg gaagagctgg gatttgcggt aaacgtcggc gaactgctga
     6601 tgaccgagtg tgaaatggtc aacggtttta tcgacccgcc ggatgagccg ccgcacttca
     6661 cgcgcggcta cgggctggta ttcggcatga gcgagcgcaa agcgatggca atggcgctgg
     6721 ttgaccgtgc gttgcaagcc ccggaatacg gcgagcacgc gacaggcccg gcgcaggatg
     6781 aagagttcgt gctggcgcat gccgacaacg tcgaagccgc aggctttgtc tcgcacctca
     6841 aactcccgca ctacgtcgat ttccaggccg aactggagct actcaaacgt ctgcaacagg
     6901 agcagaacca tggctaatct gagcggctac aactttgcct acctcgacga gcagaccaaa
     6961 cgcatgatcc gccgcgccat cttaaaagcg gtggcgatcc ccggttatca ggtaccgttt
     7021 ggcgggcgcg agatgccgat gccgtacggc tggggaactg gtggtatcca gctcaccgcc
     7081 agcgtgattg gcgaaagcga cgtgttgaag gtgattgacc agggtgcgga tgacaccacc
     7141 aacgccgtgt cgattcgcaa cttctttaag cgcgtgaccg gggtaaacac cactgaatgt
     7201 acggacgatg cgacgcttat ccagacgcgt caccgcatcc ccgaaacgcc gctgaccgaa
     7261 gatcagatca ttatcttcca ggtgccaatc ccggaaccgc tgcgctttat cgagccgcgc
     7321 gaaacggaga cccgcactat gcacgcgctg gaggagtacg gcgtgatgca ggtgaaactg
     7381 tatgaagata tcgcccgctt cggtcatatc gccaccacct atgcttatcc ggtgaaggtg
     7441 aacgggcgct acgtgatgga cccgtcgccg atcccgaaat tcgataaccc gaaaatggac
     7501 atgatgcccg ccctgcagct gttcggcgca gggcgcgaga agcgcatcta tgcggtgccg
     7561 ccgtttaccc gcgtggaaag tctcgatttc gacgatcacc cgttcaccgt tcagcagtgg
     7621 gatgagccat gcgccatctg cggatcgacc cacagctatc ttgatgaagt ggtgctggat
     7681 gacgccggaa accgcatgtt tgtctgctcc gataccgatt attgccgcca acagagcgag
     7741 gcgaaaagcc aatgaatcaa ccgttacttt cggtcaataa cctgacccac ctttacgcgc
     7801 cgggcaaagg ctttagcgat gtctcttttg atttatggcc gggggaagtg ctgggcattg
     7861 tcggggaatc cggctccggg aagaccacgc tgctgaagtc gatctccgcg cgtctgacgc
     7921 cgcagcaggg ggagattcac tatgaaaacc gttccctcta tgcaatgagc gaggccgacc
     7981 gccgtcgcct gctgcgcacc gaatggggcg tggtgcatca gcatccgctc gacggcctgc
     8041 gccgccaggt gtcggcaggc ggcaatatcg gcgagcggct gatggcgacc ggggcgcgac
     8101 attacggcga tattcgcgct accgcgcaga agtggctgga agaggtggag attcccgcca
     8161 accgtatcga cgacctgccg accacctttt ccggcggtat gcagcagcgt ttgcagattg
     8221 cccgcaacct ggtgacgcat ccgaagctgg tgtttatgga tgaaccgacc ggcgggctgg
     8281 atgtgtcggt gcaggcccgc ctgctcgacc tgctgcgcgg actggtggtg gagctgaacc
     8341 tcgcggtagt gattgtcacc cacgatttag gcgtcgcccg cctgctggcg gaccgtttgc
     8401 tggtgatgaa gcaggggcaa gtggtggaga gtgggttaac cgaccgcgtg ctcgacgacc
     8461 cgcatcatcc gtatacacag ctgctggtgt catcggtttt gcagaattga gccggtgccg
     8521 gatgcggcgt aaacgcctta tccggcctac aaatgcgctc cccgtaggtc ggataagacg
     8581 cgtcagcgtc gcatccgaca cccgaaccac gaggcgaaaa atgattaacg tacaaaacgt
     8641 cagtaaaacc ttcatcctgc accagcaaaa cggcgtgcgc ctgcccgtcc tcaaccgcgc
     8701 ctcgctcatc gtcaacgcgg gcgaatgcgt ggtgctccac ggccattccg gcagcggaaa
     8761 atcaaccctg ctacgctcgc tgtacgccaa ctatctgccc gacgaagggc aaatccagat
     8821 caaacacggt gacgaatggg tcgatctggt caccgcgcca gcgcgcaaag tggtggagat
     8881 ccgcaaaacc accgttggct gggtgagcca gtttctgcgc gtcatcccgc gtatttcggc
     8941 gctggaagtg gtgatgcagc cgctactcga taccggcgta ccgcgtgaag cctgcgccgc
     9001 taaagccgcg cgtctgctca cccgcctgaa cgtgccggaa cgcctgtggc atctggcgcc
     9061 atcgaccttc tccggcggcg aacagcagcg cgtcaacatc gcccgcggct ttatcgtcga
     9121 ctaccccatt ctgctgctcg acgaacctac cgcctcgctg gacgccaaaa acagcgccgc
     9181 cgtagtcgag ctgattcacg aagccaaagc ccgtggcgcg gccatcgtcg gcatcttcca
     9241 cgacgaagcc gtacgtaatg acgttgccga ccgcctgcac ccaatggaag cctcttcatg
     9301 attatcaata acgtcaaatt ggtgctggaa aacgaagtgg tacacggttc actggaggtg
     9361 caggacggcg aaatccgcgc ctttgccgaa agccagagcc gcctgccgga ggcgatggac
     9421 ggcgaaggcg gctggctgct gccggggctg attgagctgc ataccgataa tctggataaa
     9481 ttcttcaccc cgcgcccgaa agttgactgg cctgcccact cggcgatgag cagccacgac
     9541 gcgctgatgg tggcgagcgg catcaccacc gtactggatg ccgtggcaat tggcgacgtg
     9601 cgcgacggcg gcgatcggct ggtgaatctg gagaagatga tcaacgccat cgaagagacg
     9661 cagaaacgcg gcgtcaaccg cgccgagcac cgtctgcatc tgcgctgcga actgccgcat
     9721 cacaccacgc tgccgctgtt tgaaaaactg gtgcagcgcg agccggtgac gctggtgtcg
     9781 ctgatggacc actcgccggg ccagcgccag ttcgccaatc gcgagaagta tcgcgaatat
     9841 tatcagggca aatactccct cactgatgcg cagatgcagc agtacgaaga agagcaactg
     9901 gcgctcgccg cacgctggtc gcagccgaat cgcgaatcca tcgccgccct gtgccgcgcg
     9961 cgaaaaattg cgcttgccag ccacgatgac gcacccacgc ccacgttgct gaatctcacc
    10021 agcttggcag cgtgatcgcc gaatttccca ccacgttcga agcggcggaa gcctcgcgca
    10081 agcatggcat gaacgtgctg atgggcgcgc cgaatattgt gcgcggcggc tcgcactccg
    10141 gcaacgtggc agccagtgaa ctggcgcagc ttggcctgct ggatatcctc tcttccgact
    10201 actaccccgc cagcctgctc gatgcggcat ttcgcgtcgc cgatgacgag agcaaccgct
    10261 ttacgctgcc gcaggcggtg aagctggtga ctaaaaatcc agcgcaggcg cttaatctcc
    10321 aggatcgcgg ggtgattggc gagggcaaac gcgcggactt ggtgctggcg catcgcaagg
    10381 gcaatcacat tcatatcgac cacgtctggc gtcagggtaa aagggtgttc tgatgatggg
    10441 aaaactgatt tggttaatgg ggccgtccgg ctccgggaaa gatagcctgc tggcggaact
    10501 ccgcctgcgg gaacaaactc agctactggt ggcgcatcgc tacatcacgc gcgacgccag
    10561 cgccggaagc gaaaaccata tcgctctgag cgagcaggag ttttttaccc gcgcggggca
    10621 gaacctactg gccttaagct ggcacgctaa cggtctgtat tatggcgtcg gcgtcgagat
    10681 cgacctctgg ctgcacgccg gattcgacgt gctggtcaac ggctcacgcg cccatctgcc
    10741 gcaggcgcgg gcgcgctatc aatcggcgct gctgcccgtc tgtttacagg tttcgccgga
    10801 gatcctccgc cagcgcctgg aaaaccgtgg ccgtgaaaac gccagtgaaa ttaacgcccg
    10861 cctggcgcgc gccgcccgct atactccaca ggattgccat acgctcaaca atgacggcag
    10921 cctgcgccag tcggtcgaca cgctgctgac gctgattcat cagaaggaga aacaccatgc
    10981 ctgcttgtga gcttcgcccg gccacgcagt atgataccga cgcggtttac gcgctgattt
    11041 gtgagctaaa acaggcggag tttgaccacc acgcatttcg cgtgggcttt agcgccaatc
    11101 tgcgcgaccc gaacatgcgc tatcatctgg cgctgctgga tggcgaggtt gtcggcatga
    11161 tcggcctgca tttgcagttt catctgcatc atgtcaactg gatcggcgaa attcaggaac
    11221 tggtagtgat gccgcaggcg cgcggcctga acgtcggtag caagttgctg gcgtgggcag
    11281 aagaagaagc ccgccaggcc ggggccgaaa tgaccgagct ttcgaccaac gtgaagcgcc
    11341 acgacgcgca ccgtttctat ctgcgagaag gctacgagca gagtcacttc cgcttcacca
    11401 aggcgctgta acatgagtct gaccctcacg ctcaccggca ccggcggcgc acagggcgtt
    11461 ccggcatggg gctgcgagtg cgcggcctgc gaccgagcgc ggcgctcgcc gcagtatcgc
    11521 cgccaaccgt gcagcggcgt agtgaagttt aacgaggcga tcaccctgat cgacgccggg
    11581 ctgcacgatc tcaccgatcg ctggtctccc ggatcgttcc agcagttttt gctgacgcat
    11641 tatcatatgg atcacgtcca ggggctgttt ccgctgcgct ggggcgtggg cgatccgatc
    11701 ccgatctacg gcccgccgga tgaacagggc tgcgacgatc tgtttaaaca tcccgggctg
    11761 cttgatttca gccacacggt ggaaccgttt gtggtgtttg atttgcaggg gttgcaggtc
    11821 acgccgctgc cgcttaacca ctcaaaactc actttcggct atttactgga aacggcgcac
    11881 agccgggtgg cgtggctgtc tgacaccgct ggcttgccgg aaaaaacgct gaaattttta
    11941 cgcaataatc agccgcaggt aatggtgatg gattgcagtc acccgccgcg cgcggatgca
    12001 ccgcgtaatc actgtgattt aaataccgtg cttgcgctga atcaggttat ccgctcgcca
    12061 cgggtgattc tgacccatat cagccaccag tttgatgcgt ggctgatgga aaacgcacta
    12121 ccgtcagggt ttgaggtggg gtttgatggg atggagattg gggtggcgtg atgagaggga
    12181 atgtgcgcgc tggccccctc accctaaccc tctccccaga ggggcgaggg gaccgattgt
    12241 gctcgatatt gaatattgcg ctcgttttct ccctctcccc attggggtga ggggcgatgc
    12301 ctgctccata cccaacctca tcgcccatac tcatcttcca ttctccgctc ttcatcctcc
    12361 agttgccgac gctcctgatc aagctggcgc tggcgatcgt ccagctgcct gcggcgatct
    12421 tcaaactggc ggcggcggtc gtcatattgt ctgcgccgat cgtcgctcac ttcacgctgc
    12481 cagccgtcgt cgcgcgaatc ttcatagtct cgcccacggt cagggttata agcgtcatta
    12541 atcgcctgct gaatattacc aatagtatcg tcgataatat cagcctgggc cggaacggtg
    12601 gacagcgtga gcagggtgaa taaaagaaat agcggaaagc gtttcattag ccaacctcaa
    12661 aaagaaactc tatccacatt agtcattact catccatgca agtagtggat gaatctcaat
    12721 ttctccgctg ctctattgcc gtaatcgcct ccacgcgttg ttgatgacga ccgccttcgt
    12781 actgtgcgcc cagccacgca tccacaatca tttttgccag ttcgaggcca accactcgtg
    12841 aaccaaaagc cagtgcaact tcactggcat aatgcggata atcagtgcgc tctgacgacc
    12901 tggtgtcttt atcaatcacc tcactcattg accatgaata aaaggctggc acagcaacgc
    12961 agaatcctgc ggtgccagcg acatgaccgc ccattgctcc ggtacgtttt ttaaaaagaa
    13021 acaccggttg cattgataaa ttgtgctcag tatttgccga agtacaatat atataa
//
LOCUS       DAZPBM010000116        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPBM010000116 DAZPBM010000000
VERSION     DAZPBM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.117"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHUZQ_002979"
     CDS             <1..191
                     /locus_tag="ACHUZQ_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026617.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHUZQ_002980"
     CDS             191..1393
                     /locus_tag="ACHUZQ_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR1026618.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUZQ_002981"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUZQ_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1026619.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHUZQ_002982"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHUZQ_002982"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1026620.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUZQ_002983"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUZQ_002983"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1026621.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUZQ_002984"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUZQ_002984"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1026622.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUZQ_002985"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUZQ_002985"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1026623.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUZQ_002986"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUZQ_002986"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1026624.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUZQ_002987"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUZQ_002987"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1026625.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZPBM010000117        12563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPBM010000117 DAZPBM010000000
VERSION     DAZPBM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.118"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHUZQ_002988"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHUZQ_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1026626.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHUZQ_002989"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHUZQ_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1026627.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHUZQ_002990"
     CDS             1797..1970
                     /locus_tag="ACHUZQ_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026628.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHUZQ_002991"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHUZQ_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1026629.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHUZQ_002992"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHUZQ_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1026630.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHUZQ_002993"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHUZQ_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1026631.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHUZQ_002994"
     CDS             complement(4268..5191)
                     /locus_tag="ACHUZQ_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1026632.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="ACHUZQ_002995"
     CDS             5408..6709
                     /locus_tag="ACHUZQ_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1026633.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="ACHUZQ_002996"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="ACHUZQ_002996"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1026634.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="ACHUZQ_002997"
     CDS             8771..8995
                     /locus_tag="ACHUZQ_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1026635.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="ACHUZQ_002998"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="ACHUZQ_002998"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1026636.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHUZQ_002999"
     CDS             complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHUZQ_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1026637.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="ACHUZQ_003000"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="ACHUZQ_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1026638.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="ACHUZQ_003001"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="ACHUZQ_003001"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1026639.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2927 c         3146 g         3282 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAZPBM010000118        12507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPBM010000118 DAZPBM010000000
VERSION     DAZPBM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.119"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHUZQ_003002"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHUZQ_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1026640.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHUZQ_003003"
     CDS             433..621
                     /locus_tag="ACHUZQ_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1026641.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHUZQ_003004"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHUZQ_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1026642.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHUZQ_003005"
     CDS             816..989
                     /locus_tag="ACHUZQ_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1026643.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHUZQ_003006"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHUZQ_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUZQ_003007"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUZQ_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR1026644.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHUZQ_003008"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHUZQ_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1026645.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUZQ_003009"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUZQ_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1026646.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHUZQ_003010"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHUZQ_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1026647.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHUZQ_003011"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHUZQ_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHUZQ_003012"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHUZQ_003012"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1026648.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUZQ_003013"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUZQ_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1026649.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUZQ_003014"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUZQ_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1026650.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3016 a         3205 c         3273 g         3013 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgtggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attattt
//
LOCUS       DAZPBM010000119        12128 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPBM010000119 DAZPBM010000000
VERSION     DAZPBM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12128
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.120"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHUZQ_003015"
     CDS             complement(<1..101)
                     /locus_tag="ACHUZQ_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026651.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUZQ_003016"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUZQ_003016"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1026652.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUZQ_003017"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUZQ_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1026653.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUZQ_003018"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUZQ_003018"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1026654.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUZQ_003019"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUZQ_003019"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1026655.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUZQ_003020"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUZQ_003020"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1026656.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUZQ_003021"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUZQ_003021"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1026657.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHUZQ_003022"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHUZQ_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1026658.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHUZQ_003023"
     CDS             9871..10449
                     /locus_tag="ACHUZQ_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1026659.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHUZQ_003024"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHUZQ_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1026660.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         2683 a         3296 c         2887 g         3262 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaa
//
LOCUS       DAZPBM010000120        12116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPBM010000120 DAZPBM010000000
VERSION     DAZPBM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.121"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..478
                     /locus_tag="ACHUZQ_003025"
                     /pseudo
     CDS             1..478
                     /locus_tag="ACHUZQ_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            801..2189
                     /gene="narU"
                     /locus_tag="ACHUZQ_003026"
     CDS             801..2189
                     /gene="narU"
                     /locus_tag="ACHUZQ_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1026661.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2271..6011
                     /gene="narZ"
                     /locus_tag="ACHUZQ_003027"
     CDS             2271..6011
                     /gene="narZ"
                     /locus_tag="ACHUZQ_003027"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR1026662.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6008..7552
                     /gene="narH"
                     /locus_tag="ACHUZQ_003028"
     CDS             6008..7552
                     /gene="narH"
                     /locus_tag="ACHUZQ_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1026663.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7552..8247
                     /gene="narW"
                     /locus_tag="ACHUZQ_003029"
     CDS             7552..8247
                     /gene="narW"
                     /locus_tag="ACHUZQ_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1026664.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8244..8924
                     /gene="narI"
                     /locus_tag="ACHUZQ_003030"
     CDS             8244..8924
                     /gene="narI"
                     /locus_tag="ACHUZQ_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1026665.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYACFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9003..9896
                     /locus_tag="ACHUZQ_003031"
     CDS             9003..9896
                     /locus_tag="ACHUZQ_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR1026666.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9992..10837)
                     /gene="nhoA"
                     /locus_tag="ACHUZQ_003032"
     CDS             complement(9992..10837)
                     /gene="nhoA"
                     /locus_tag="ACHUZQ_003032"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR1026667.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11010..11579
                     /locus_tag="ACHUZQ_003033"
     CDS             11010..11579
                     /locus_tag="ACHUZQ_003033"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR1026668.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11583..11810)
                     /gene="pptA"
                     /locus_tag="ACHUZQ_003034"
     CDS             complement(11583..11810)
                     /gene="pptA"
                     /locus_tag="ACHUZQ_003034"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR1026669.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11910..>12116)
                     /locus_tag="ACHUZQ_003035"
     CDS             complement(11910..>12116)
                     /locus_tag="ACHUZQ_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1026670.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2905 a         3103 c         3131 g         2977 t
ORIGIN      
        1 aaaagtttaa gactggacaa caataaaata acccatattg atactaataa tacatccgat
       61 attggaactt tatttcccat aataaaacag agcaaaaact taaatttttt aaatgtttct
      121 gggaagaaca attgccctac tatgcagctc atgttattta atttgttttc cccaacactt
      181 aagcttaata ctggcctggc aattctttcg cctggtgcat ttgaagttca ctctgacgga
      241 atagatgtgg ataacgaatt gtttcactat cctattaaaa atagcatata ccccatataa
      301 tatacacact tacaagacag aggaagttgt aaaccagagg aatataaaag ttaaaaacat
      361 gaccttagat gaaataaaca atacttactg taataacgat tattacaatc aggcaataag
      421 agaggaaccg atagaccttc tggacagatc gttttcctcc agttcatggc ctttttagtg
      481 aataaatcat cactaattct ataagtgtct ggtcttgttg ataatatagt taatatgtaa
      541 ccctttagat tcacgatctg aagggttgaa acatgacata cgttcaaaga ttaaccccgt
      601 ttgaaggtta gcttgttagt tacatttagt aacacatatc tggctactta atccccattc
      661 tgcccccgcg gttacgcttt cttatctatt ctttcaatca gcacttcacc caattcatcc
      721 ttaattctaa taactggcta ttgcgcctta cctcctgttt tgtctttttt cattaccaat
      781 gtgtgcatgt gaggaacaat atggcactgc aaaatgagaa aaatagtcgt tatcttttac
      841 gcgactggaa accagaaaat ccggccttct gggaaaataa aggaaaacat attgctcgaa
      901 gaaacctctg gatatcagtc agttgtctac ttcttgcctt ctgtgtctgg atgctattta
      961 gcgcagttac cgttaatctc aataaaatcg gttttaattt tactaccgat caactctttt
     1021 tattaaccgc attaccctcc gtttctggcg cattattgcg tgttccctac tcctttatgg
     1081 tgcctatatt cggtggacgc cgatggacgg tttttagtac tgcaatcctg attattcctt
     1141 gcgtctggct cggaattgcc gtgcaaaatc cgaatactcc ttttgggata tttatcgtta
     1201 tcgctttgct atgcggtttt gcaggtgcaa actttgcttc gagcatgggc aatatcagtt
     1261 tcttctttcc aaaagccaaa caagggagcg ctcttgggat taatggcgga ttaggaaact
     1321 taggtgtaag tgtaatgcag ctggttgcac cgctggtcat ttttgtacct gtatttgcct
     1381 ttctcggcgt caatggcgta ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg
     1441 catggatttg ggtaccgcta ctggcgattg ccacgatcgc cgcctggtca gggatgaatg
     1501 atatcgccag ttcacgcgcc tcaattgacg accagctccc tgtcttacaa cgcctgcatc
     1561 tctggctgct gagcctgctt taccttgcca ccttcggttc gtttatcggt ttttctgcgg
     1621 gttttgccat gctggcaaaa acccagttcc cggatgtgaa tattctgcgc ctggcgttct
     1681 ttggcccatt tatcggtgcc atcgcgcggt cggttggtgg tgctatttcc gataaattcg
     1741 gcggcgtgcg ggtgacgttg atcaacttta tttttatggc gattttcagt gccctgctgt
     1801 tccttacctt accgggcaca ggctccggta atttcatcgc cttttacgcc gtatttatgg
     1861 ggctgtttct gactgcgggt ctgggaagtg gttctacttt ccagatgatc gccgtcatct
     1921 ttcgccagat aaccatttat cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga
     1981 aagaagccat caccgaaacg gcggcggctc tgggctttat ctcagccatt ggcgcagtgg
     2041 gcggcttttt tattccgcag gcgtttggca tgtcgctcaa tatgaccggc tctccggtcg
     2101 gcgcgatgaa agtgttttta atcttctaca tcgtttgtgt gctgctgacc tggctggttt
     2161 atggtcggcg gaagttcagc caaaaataag agatcgatgc ctgatgtaca accgtgtcgc
     2221 atccgacaag gcatcagatc ccaattcatt cctggagcag gagtcatgtc atgagtaaac
     2281 ttttggatcg ctttcgctac ttcaaacaaa agggcgaaac ctttgccgat ggtcacggac
     2341 aggtgatgca tagcaaccgc gactgggagg acagctatcg ccagcgttgg cagttcgaca
     2401 aaatcgtgcg ttccacccac ggtgttaact gtacaggctc ctgtagctgg aaaatctacg
     2461 ttaaaaatgg tctggtgacc tgggaaatcc aacagaccga ctacccgcgc actcgccctg
     2521 accagcccaa tcatgaacct cgcggctgcc cgcgtggcgc aagttactcc tggtatctct
     2581 acagcgctaa ccgcctgaaa tacccgctca ttcgtaaacg actgattgaa ctgtggcgtg
     2641 aagctctcaa gcaacacagc gatccggtac tggcgtgggc atcgattatg aacgatccgc
     2701 aaaagtgctt aagctacaaa caagtgcgtg ggcgcggcgg gtttatccgc tccaactggc
     2761 aggaacttaa ccaactgatt gccgccgcta acgtctggac catcaaaatc tatggtccgg
     2821 atcgcgttgc cggtttctcg ccgatcccgg cgatgtcgat ggtgtcatac gctgccggaa
     2881 cgcgttatct gtcgctgtta ggcggcacct gtttaagctt ctacgactgg tattgcgacc
     2941 tgccgcccgc ctcgccgatg acctggggcg aacaaaccga cgtgccggaa tctgccgact
     3001 ggtataactc cagttacatc atcgcctggg ggtctaacgt accgcagaca cgtacgccgg
     3061 acgcccactt ctttaccgaa gtacgttaca aaggcaccaa aactatcgcc attacccctg
     3121 actactctga agtggccaaa ttgtgcgacc agtggctggc accgaaacaa ggcactgata
     3181 gcgccctggc gatggcaatg ggccatgtga ttttaaaaga gtttcatctc gataatccca
     3241 gcgactactt tatcaactac tgccgccgct atagcgacat gccgatgctg gtaatgctgg
     3301 aacctcgcga cgatggtagc tacgtacccg ggcgcatgat ccgcgcgtct gacctggtag
     3361 atggactagg cgaaagcaat aatccgcagt ggaaaaccgt ggcggttaat accgcaggtg
     3421 aattggtagt gccgaatggt tcgattggtt tccgctgggg agaaaaaggc aaatggaatc
     3481 tggaatccat tgccgccggt aaggaaaccg aattgtcgtt aaccctgctc ggtcaacatg
     3541 acgctgttgc aggcgtggcc ttcccctact ttggcggcat tgaaaatccg cattttcgca
     3601 gcgtaaaaca caatccggtg ctggtgcgcc aattgcccgt taaaaacctg acgttagtcg
     3661 atggcaacac ctgtccggtg gtcagcgttt atgatttggt gctggcgaat tacggcctcg
     3721 atcgcgggct ggaagatgaa aacagcgcga aagattacgc tgaaatcaaa ccgtacaccc
     3781 cagcctgggg tgagcaaatt accggcgtac cgcgccagta tattgaaacc atcgcccgcg
     3841 aatttgccga taccgcccat aaaacgcatg gacgctcaat gattatcctc ggcgctggcg
     3901 tcaaccactg gtatcacatg gatatgaact accgtgggat gatcaatatg ctgatcttct
     3961 gcggttgtgt cgggcaaagc ggcggcggct gggcgcacta tgtcggtcag gaaaaactgc
     4021 gcccacaaac cggctggttg ccgctggcct ttgcgctcga ctggaaccgc ccaccgcgcc
     4081 agatgaacag tacctcgttt ttctacaatc attccagcca atggcgctat gaaaaagtct
     4141 ctgctcagga gttactttca ccgcttgccg atgccagtaa gtacagcggt catctgattg
     4201 atttcaacgt tcgcgccgaa cgcatgggct ggctaccttc tgcgccgcag ctggggcgta
     4261 acccgctcgg gattaaagct gaagccgaca aggccggatt atcccccaca gaatttaccg
     4321 cccaggcgct gaaatcgggc gatttacgta tggcctgcga acaaccagat agcggcagca
     4381 atcatccgcg taatttgttt gtctggcgct ctaacctgct tggctcctcc ggcaaaggcc
     4441 acgagtatat gcagaagtat ctgctgggga ccgaaagcgg gattcagggc gaggaactcg
     4501 gtgccagcga cgggatcaaa ccggaagaag tcgagtggca aactgcggcg attgaaggca
     4561 agctcgacct gctggtgacg ctcgacttcc gcatgtccag tacctgcctg ttctccgata
     4621 tcgttctgcc caccgccacc tggtacgaaa aagacgatat gaacacctcg gatatgcatc
     4681 cgtttattca tccgctttct gcggcggtcg atccggcgtg ggaatcacgc agcgactggg
     4741 aaatctacaa aggtatcgcc aaagcatttt cgcaagtgtg cgtgggtcat cttggcaaag
     4801 aaaccgacgt ggtattacaa cccctactgc atgactctcc ggcagagctc tcacagccgt
     4861 gtgaagtgct cgactggcgc aaaggcgaat gcgatctgat tccaggcaaa accgcgccga
     4921 atattgtggc ggtggagcgc gactaccctg ctacgtatga acgctttacc tcgctcgggc
     4981 cattgatgga caaacttggc aatggtggta aagggatttc gtggaatacc caggatgaaa
     5041 tcgatttcct cggcaaactc aattacacca agcgtgatgg cccggcacag gggcgtccgc
     5101 tgattgacac cgccattgac gcttcagaag tgattctggc actggcaccg gaaaccaacg
     5161 gtcatgttgc agtcaaagcg tggcaggcgc tgggcgagat caccgggcgc gaacataccc
     5221 atctggcgct gcacaaagag gacgagaaga ttcgctttcg cgatattcag gcgcagccgc
     5281 gtaaaattat ctccagcccc acatggtccg gtctggaaag cgatcacgtc tcctataatg
     5341 cgggatacac caacgttcat gagttaattc cgtggcgcac gctgtcggga cgccagcagc
     5401 tctatcagga tcatccgtgg atgcgtgctt ttggtgaaag cctggtggct tatcgcccgc
     5461 ctatcgacac ccgtagcgtc agtgagatgc gccagatccc gccaaacggc ttcccggaaa
     5521 aagcacttaa cttcctgacg ccgcaccaga aatggggcat tcactcaacc tacagtgaaa
     5581 acctgctaat gctgacgctc tctcgcggtg gaccgattgt ctggatcagc gaaaccgatg
     5641 cccgagaact gaccattgtc gataacgact gggtggaagt ctttaacgcc aacggcgcgc
     5701 tgacggctcg tgcggtggtc agccaacgtg taccgccggg tatgaccatg atgtatcacg
     5761 cccaggaacg cattatgaat attcctggtt cggaagtaac tggcatgcgc ggcggcattc
     5821 ataactcggt cacccgtgtt tgcccgaaac cgacgcatat gattggcggt tacgcgcagc
     5881 tggcctgggg ctttaactac tacggcaccg tcggctcgaa ccgcgacgaa ttcatcatga
     5941 tccgcaagat gaagaacgtt aactggctgg atgatgaagg tcgcgatcag gtacaggagg
     6001 cgaaaaaatg aaaatacgtt cgcaagtcgg catggtgctt aacctcgaca aatgtatcgg
     6061 ttgccatacc tgttcggtga cctgtaaaaa cgtctggacc ggacgcgaag gcatggagta
     6121 cgcatggttt aacaacgtcg aaaccaaacc gggcattggt tatccgaaaa actgggaaga
     6181 tcaggaagag tggcaaggcg gctgggtccg cgatgtgaat ggcaagatac gcccgcgtct
     6241 gggcagcaag atgggcgtaa taaccaaaat cttcgccaac ccggtggtgc cgcagattga
     6301 tgattactac gaacctttca ccttcgacta cgaacatttg catagcgcac cggaaggcaa
     6361 acatatccct actgctcgcc cgcgttcgct gattgacggt aagcggatgg acaaagtgat
     6421 ctgggggcca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     6481 caacttcgag gccatgcaaa aggagatgta cgggcagttt aaaaacacct tcatgatgta
     6541 cctgccgcgc ctgtgcgaac actgcctcaa tcccagttgc gtggcgacct gcccaagcgg
     6601 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     6661 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     6721 caagtcagaa aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     6781 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     6841 cattgaggaa gcggcgagca ccgagcgcga agttgacctc tatgaacgtc agtgcgaagt
     6901 gttcctcgat ccacacgatc cctcagtgat cgaggaagcc ctgaaacaag gtattccaca
     6961 aaacgtgatt gaagctgccc agcgttcgcc tgtctacaaa atggcgatgg actggaaact
     7021 ggcgctaccg ctgcaccctg aatatcgcac cctgccaatg gtctggtacg ttcctccgct
     7081 gtcgccgatt cagtcctacg cagatgcggg cggtttgccg aaaagcgaag gcgtgctgcc
     7141 cgccatcgaa agcctgcgta ttccggtgca atatctcgcc aatatgttga gtgccggcga
     7201 taccggtccg gtactgcggg cgctgaaacg gatgatggcg atgcgccact atatgcgttc
     7261 acaaaccgtg gaaggcgtta ctgatactcg tgccatcgac gaagtaggcc tgagcgtcgc
     7321 ccaggtcgaa gaaatgtatc gctatctcgc cattgccaac tatgaagatc gttttgttat
     7381 cccgaccagc catcgggaaa tggcgggcga tgccttcgca gaacgcaacg gctgcggttt
     7441 tacctttggc gacggttgcc acggttcgga cagtaaattc aacctgttca acagtagccg
     7501 tatcgatgcc atcaacatca ccgaagtgcg cgacaaagcg gagggcgaat aatgcagatc
     7561 ctcaaagtga tcggcctgtt gatggagtat ccggatgagc tgttgtggga atgcaaggag
     7621 gacgcgctgg cgttgatccg ccgcgacgcg ccgatgctta cggatttcac ccacaacctg
     7681 cttaacgcgc cgctgctgga taaacaggcc gaatggtgcg aagtgtttga ccgcgggcgc
     7741 accacgtcgc tgctgctgtt cgaacatgtt catgccgagt cccgcgatcg cggccaggca
     7801 atggtggacc tgctggcgga gtatgaaaag gtcggcctgc agctggattg tcgggaactg
     7861 cccgattatc taccgctgta tctggagtat ttaagcgtac tgcctgacga tcaggcgaaa
     7921 gaaggattgc tcaatgttgc gccgatcctc gccctgcttg gcggtcgctt aaaacaacgc
     7981 gaggcaccgt ggtacgcgtt gtttgatgct ctgctgcaac tggcgggaag tatactttca
     8041 agtgacagtg tcacaaaaca agtaaacagc gaagagcgcg atgacacccg ccaggcgctt
     8101 gatgcagtgt gggaagagga acaggtgaag tttattgaag ataacgccac ggcgtgtgac
     8161 agctcgccgc ttaatcaata tcagcgacgc tttagccagg atgtcgcgcc gcagtatgtc
     8221 gacatcagtg cgggaggtgg gaaatgattc agtatctgaa cgtctttttt tacgatatct
     8281 acccgtacat ttgtgcgacg gtgtttttcc tcggcagctg gctgcgctac gactacgggc
     8341 agtacacctg gcgcgcctcc tcaagtcaaa tgctcgataa acgcgggatg gtgatatggt
     8401 cgaatctgtt ccatatcggc attttgggga ttttcttcgg gcacttgttc ggcatgttaa
     8461 cgccgcactg gatgtacgcg tgttttttgc cagtggcagc gaaacaactc atggcgatgg
     8521 tgctcggcgg tatttgcggt gtgttgacgt tgattggcgg cgcaggactg ctgtggcgca
     8581 gtctgacaaa tcagcgcgtg cgtgccactt ccactacgcc ggatatcatc atcatgagca
     8641 ttttgctgat ccagtgtctg ttggggctaa gtaccatacc gttttcagca cagtatcctg
     8701 atggtagcga aatgatgaag ctggtgggtt gggcgcaaag cattgtcact ttccgtggtg
     8761 gatcgtcaga gatgctcaac ggcgtagcgt tcgtcttccg cctgcatctg gtgttgggaa
     8821 tgaccatctt cctgctcttc ccgttcaccc gactggtgca tgtgtggagc gcgccgtttg
     8881 agtactttac tcgtcgatat caaattgtgc gttcgcggcg ataatttcac cctggccgga
     8941 tagcccttcg ctctccggcc acttgcaggc tatgatagtt ctccagtcac aggagaacgt
     9001 taatgaaacc gcaggtgtac cacgtcgatg cttttacctc tcaaccattt cgcggcaatt
     9061 ctgccggtgt tgttttcccc gccgataatc ttagcgaagc acaaatgcag cttatcgccc
     9121 gcgagttagg ccattcggaa actgcttttc tgctgcacag cgacgacagc gatgtgcgca
     9181 tccgctactt tacgccaacg gttgaagtgc cgatttgcgg tcacgcgaca gtagctgcgc
     9241 actatgtacg tgcgaaggtg ttaggtttag gaaattgcac gatctggcaa acatcgctgg
     9301 caggaaaaca tcgcgtgact atcgaaaagc acaacgatga ttatcgtatt tcgctggaac
     9361 aaggtacgcc gggctttgaa ctgccgctgg agggtgaagt acgcgcggcg attatcaacg
     9421 cattgcatct tactgaagac gatattctgc cgggtttgcc gatccaggtc gcgacgaccg
     9481 ggcattctaa agtgatgatc ccattgaagc cggaagtgga tatcgacgct ctttcgcctg
     9541 accttaatgc gctgaccgct atcagtaaac agattggctg caatggtttc ttcccgttcc
     9601 agatccgtcc aggcaaaaac gaaaccgatg gtcgtatgtt ctcgcctgcg attggtattg
     9661 tggaagatcc ggtgactggc aatgccaatg gcccgatggg tgcatggttg gtacatcaca
     9721 acgtattgcc ccacgatggc aacgtgttgt gtgttaaagg ccatcagggg cgcgcattgg
     9781 ggcgtgacgg catgattgaa gtgacggtga caattcgtga taaccaaccg gaaaaagtca
     9841 ccatttcagg cacagcggtg attttgtttc atgccgaatg ggcaattgaa atctgaaatc
     9901 gttgcctgat gcgcttcgcc tgtcaggtct acaaccgggc taatgtatgc cggataaggc
     9961 gtttacgccg catccggcaa cctgacataa attattttcc cgcctccgga tgcgtatcaa
    10021 acgccgccat caccagcgct aactcatcca cagtaaagcc atgtttcgca tcatccacgc
    10081 ccagaccaaa ctgttcctgc atcacggcat ataacgacgc cacatccggt agatttcgct
    10141 gctccaccgc gtgcccattt tcataatggg taaaatgaaa attggtcagt gtcagcttgc
    10201 cgccgtccgg caaatggcgg cacatcagca aatgatggcg aaaatgcgac tgcggccagt
    10261 gcgccgacca gaaattgccc atcacataat cgctttgttg ctgctcgcag agatcgaagc
    10321 ggtacatcgg ctgccaatgc tgatgatgat taaactgcaa cacccaatca tcaccctcct
    10381 gcaacaaccg atactctccg tgtggcgtgg tctgcacgag atcggaaact aaacgaatcg
    10441 gcgcggttag cgtctgccca ccgaaaccga catcagcaat ccatttttcc ccttccagtt
    10501 ccaccaacag caaacgatgg gtgcgcggcg gtaatgctgg cggatttgat aacactacgc
    10561 gccctaacaa gctgcgaacg ttaaacccca gctcgcgtaa cacccgctca aacacgccat
    10621 tctgctcaaa acagtaaccg ccacgacggg ctatcaccag tttctcttcc agcgattgat
    10681 catcaagctg tatttccctc ggcagcaaaa cgtcaaggtt ttcaaacgga atggtgcaat
    10741 tgtgtttcag gtgcaatgca cgaagcgtat caatattgac cgcagcagct cccgaccagt
    10801 taatacgggc aaaatagtga ttcagaatgg gcgtcatcag gcgtttccct tagtggtttt
    10861 ctcaacttat aacctggaat agacgccgca ccggctattt tgtgcgatcg cggacatgca
    10921 tcacgtttgc aaatcgccaa tcccgttaat ttcgttactt attgcgttac ggactacgct
    10981 taaaaaaacc ttcttcgcga ggcgatacca tgagccgatt catccccata gaattacacc
    11041 atgccagccg tctgttaaat catggcccaa ctgtcttgat caccagtttc gatgagcaat
    11101 cccagcggcg taacattatg gctgcagcct ggtcaatgcc ggtggagttt gaacctccac
    11161 gcgtggcaat tgtggtagat aaatcgacat ggaccaggga gttgattgaa cgtaacggta
    11221 agtttggcat cgttatcccg ggcgttgcag caactaactg gacgtgggcg gtgggaagtg
    11281 tgtcggtgcg tgaagaagat aaatttaatt gctatggcat tccggtggtg aaaggtccgg
    11341 tgcttggttt gccacttgtt gaagagaaat gcctggcgtg gatggagtgc cgcctgctac
    11401 ctgcgacttc tgcgcaacaa aaatacgaca cgctgtttgg cgaggtggta tcagcagcgg
    11461 cagacgcacg ggtatttgtc gaaggccgct ggcagtttga tgatgataag ctcaatacgt
    11521 tgcatcattt aggtgctggc acgtttgtta ctagcggcaa gcgtgttacg gcaggttaag
    11581 cattacatgc tgtaaccggg tttctttatc aaagcctcca tttggggctc gatttcggca
    11641 tcccagatag cttgccaaga ttctggttga atctgctgta gagcaatgct tatcgagcta
    11701 tctttactgt tcagatggcg aataataacg tcggtaatat ctgcagcaag tgctgctttt
    11761 tgttgttcgt ccagttcacg cggaaagcat ttaatgtcga tgtgcggcat gattacgctc
    11821 cttttgaaag atgtcagtaa aattaacaca cattgtgaat gtcatgtgtc cggtaaagcg
    11881 tttatacgac aataatgccc agggcaagat tacgaaagcc cgctccccgt aaggactgac
    11941 gccaggtagt ttctgcccat ggctgctttt cgcatcttac gtcttaaccc tgccttgaat
    12001 accttatcat tcgtcaaaat attaatagca atgtgccgta tccctgaaaa taattctgct
    12061 gcatttcctc ttcttatttt gcagtcgtct tcattcatta ccacgtccag acgcca
//
LOCUS       DAZPBM010000121        12080 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPBM010000121 DAZPBM010000000
VERSION     DAZPBM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12080
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.122"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUZQ_003036"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUZQ_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR1026671.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUZQ_003037"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUZQ_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR1026672.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUZQ_003038"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUZQ_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR1026673.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHUZQ_003039"
     CDS             complement(3668..3898)
                     /locus_tag="ACHUZQ_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR1026674.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHUZQ_003040"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHUZQ_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHUZQ_003041"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHUZQ_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR1026675.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHUZQ_003042"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHUZQ_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHUZQ_003043"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHUZQ_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR1026676.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHUZQ_003044"
     CDS             7629..8642
                     /locus_tag="ACHUZQ_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1026677.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKI
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHUZQ_003045"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHUZQ_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHUZQ_003046"
     CDS             9533..10327
                     /locus_tag="ACHUZQ_003046"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1026678.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHUZQ_003047"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHUZQ_003047"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR1026679.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            11983..>12080
                     /locus_tag="ACHUZQ_003048"
     CDS             11983..>12080
                     /locus_tag="ACHUZQ_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFR1026680.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         2946 a         2913 c         3223 g         2998 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaatc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attagcataa ggctttagag
    11941 tatcgttatt aaactaataa tttataagta gaagtattaa caatgaaagt tcaattgtac
    12001 aaattcacgg aagataaaaa taaaaccctt accttcagat ggacgaaaaa acactttgag
    12061 ttttgtatgg ataataaaat
//
LOCUS       DAZPBM010000122        11949 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPBM010000122 DAZPBM010000000
VERSION     DAZPBM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.123"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUZQ_003049"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUZQ_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1026681.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1241..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUZQ_003050"
     CDS             complement(1241..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUZQ_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1026682.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLM"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUZQ_003051"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUZQ_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1026683.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUZQ_003052"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUZQ_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1026684.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUZQ_003053"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUZQ_003053"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1026685.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUZQ_003054"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUZQ_003054"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1026686.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHUZQ_003055"
     CDS             complement(6574..7338)
                     /locus_tag="ACHUZQ_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1026687.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUZQ_003056"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUZQ_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1026688.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHUZQ_003057"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHUZQ_003057"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1026689.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHUZQ_003058"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHUZQ_003058"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1026690.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT         2798 a         3348 c         3166 g         2637 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctacatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcat
//
LOCUS       DAZPBM010000123        11915 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPBM010000123 DAZPBM010000000
VERSION     DAZPBM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.124"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            144..710
                     /locus_tag="ACHUZQ_003059"
     CDS             144..710
                     /locus_tag="ACHUZQ_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1026691.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            866..2898
                     /locus_tag="ACHUZQ_003060"
                     /pseudo
     CDS             866..2898
                     /locus_tag="ACHUZQ_003060"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2875..3219
                     /locus_tag="ACHUZQ_003061"
     CDS             2875..3219
                     /locus_tag="ACHUZQ_003061"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR1026692.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3234..3965
                     /locus_tag="ACHUZQ_003062"
     CDS             3234..3965
                     /locus_tag="ACHUZQ_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1026693.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3962..5023
                     /locus_tag="ACHUZQ_003063"
     CDS             3962..5023
                     /locus_tag="ACHUZQ_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1026694.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5175..6221
                     /locus_tag="ACHUZQ_003064"
     CDS             5175..6221
                     /locus_tag="ACHUZQ_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1026695.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6218..7009)
                     /gene="nei"
                     /locus_tag="ACHUZQ_003065"
     CDS             complement(6218..7009)
                     /gene="nei"
                     /locus_tag="ACHUZQ_003065"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1026696.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7045..7779)
                     /gene="pxpA"
                     /locus_tag="ACHUZQ_003066"
     CDS             complement(7045..7779)
                     /gene="pxpA"
                     /locus_tag="ACHUZQ_003066"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1026697.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7769..8701)
                     /gene="pxpC"
                     /locus_tag="ACHUZQ_003067"
     CDS             complement(7769..8701)
                     /gene="pxpC"
                     /locus_tag="ACHUZQ_003067"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR1026698.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8695..9351)
                     /gene="pxpB"
                     /locus_tag="ACHUZQ_003068"
     CDS             complement(8695..9351)
                     /gene="pxpB"
                     /locus_tag="ACHUZQ_003068"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1026699.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9374..10117)
                     /gene="ybgI"
                     /locus_tag="ACHUZQ_003069"
     CDS             complement(9374..10117)
                     /gene="ybgI"
                     /locus_tag="ACHUZQ_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1026700.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10388..11869
                     /gene="dtpD"
                     /locus_tag="ACHUZQ_003070"
     CDS             10388..11869
                     /gene="dtpD"
                     /locus_tag="ACHUZQ_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1026701.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2869 a         3173 c         2901 g         2972 t
ORIGIN      
        1 acatttaatt cataccatca attaattact tttacattta ataaaattaa taatattttt
       61 aagaaaaact aaaaacattc gacaatcaaa taagatattc ttgcaccgat atactttttt
      121 cttttctgtt aaaggactca gttatgttta aaggacaaaa aacattggcc gcactggccg
      181 tatctctgct gttcacagca cctgtttatg cggctgatga aggatccggt gaaattcact
      241 ttaaaggtga agttattgaa gcaccgtgtg aaatacatca ggatgatatt gataaagagg
      301 ttgaactcgg tcaggtgacc accagccaca ttaatcagtc acatcacagc gatgccgttg
      361 ctgtcgacct gcgcttagtc aactgtgatc tggaaaactc cagcaacggt tccggtggca
      421 agatttctaa agttgcagtc acctttgata gctcagcgaa aaccaccggc gcagatccga
      481 ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag
      541 atcaaagcaa tatcgtttta ggcaccgcta ctccagatat cgacctggct ccgacctcca
      601 gcgaacaaac gctgaatttc tttgcctgga tggaacaaat tgatcaggct acacctgtaa
      661 cgccaggcgc agttacggcg aatgcaacct acgtgctgga ttataaataa gtattacttt
      721 ggcggcgcta tcgccgcctt tcataaaata tttatcaggt atggacaccg tgaatattta
      781 tcgactctct tttgtatcct gcctggtcgt ggcgatgcct tgcgcattgg cggtcgaatt
      841 caaccttaat gttctcgata aatcgatgcg cgaccgcatt gatatttcat tattaaagga
      901 aaaaggagtc attgctcccg gtgagtattt tgttagcgtt gcggtaaata ataaccaaat
      961 cagtaacggg caaaagatta actggcacaa aaatgacgat aaaaccattc cgtgcatcaa
     1021 tgatttactg gtcgataaat ttggcttaaa acctgaagtc cgtcagtcgt taccattgat
     1081 aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa
     1141 tcagcaactg aatattagta ttccgcaagc ctggctggcg tggcactcag aaaactggcc
     1201 cccaccctcc acatggaaag aaggtgtcgc cggtatcctg atggattaca acttgtttgc
     1261 cagcagctac cgcccacagg acggcagcag cagcactaac ctgaacgcct acggtaccac
     1321 cggaattaac gccggggcat ggcgcttacg tagtgattat cagttgaatc agactgatag
     1381 cgatgataac catgaacagt caggcggaat atcgcgcacc tatctttttc gtccattacc
     1441 gcaattaggc tctaaattaa ccctcggcga aacggatttt agttccaata ttttcgacgg
     1501 tttttcttat accggcgcgg cactggcaag tgacgagcga atgttgccat gggaactacg
     1561 cggctacgcc ccacaaatta gcggtattgc acagaccaat gccacggtga cgatcagtca
     1621 atcaggccgc gtcatttacc agaaaaaagt cccaccaggc ccatttatca ttgacgacct
     1681 taatcagtct gttcagggca cactggatgt caaagtgacg gaagaagatg gtcgggtgaa
     1741 caatttccag gtttcggcag catcgacgcc cttcctgact cgtcagggac aggttcgcta
     1801 taaactggcc gcgggtcagc cacggccctc catgtcacat caaactgaaa atgaaacctt
     1861 ttttagcaat gaagtttcct gggggatgct gtcaaacacc tcgctgtacg gcggcctgct
     1921 gctttctggt gatgactacc attctgccgc aatgggtatt gggcaaaata tgctgtggct
     1981 tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat tttgatactc agcaggacga
     2041 gcggggctta agctaccgtt ttaattacag caaacaagtg gatgccacta acagcacgat
     2101 ttccctcgcc gcttatcgtt tctccgatcg tcattttcac agctacgcca actatctgga
     2161 tcacaaatac aacgacagcg atgcgcagga cgaaaaacag acgatcagct tattgtgggc
     2221 caaccgatta ccccactaaa cctcaatctt tacgccaacc tgctacatca aacctggtgg
     2281 aatgcagacg cctccacgac cgccaacatc acggcaggtt ttaatgttga tattggtgac
     2341 tggagagata tctctatttc gacgtcattc aatacaaccc attacgaaga taaagatcac
     2401 gacaaccaga tttacctgtc gatttcgctc cccttcggta acggtggtcg ggttggctat
     2461 gacatgcaaa acagtagcca cagcaccaca caccgcatgt cgtggaacga tacgctggat
     2521 gaacgtaata gctggggcat gtctgccgga ctgcaatccg accgtcctga caatggagcc
     2581 caggtgagcg gtaactatca gcacctgagt tcagcgggtg agtgggatat ttctggtacc
     2641 tatgccgcca atgattacag ttccgtcagc agcagctgga gcggttcttt caccgcaacc
     2701 caatatggtg cagcgtttca tcgccgcagc tccaccaatg aaccacgcct gatggtcagc
     2761 accgatggcg tggcagatat tccggttcag ggcaatctcg actacaccaa ccattttggc
     2821 attgcggtgg tgccgttgat ttccagttac cagccttcca ccgtggcggt gaacatgaat
     2881 gacttacccg acggcgtaac agttgcagaa aacgttatca aagaaacgtg gattgaaggc
     2941 gcgataggtt acaaatcact ggcttcccgt tccggtaaag acgttaacgt catcattcgc
     3001 aacgccagcg gtcagtttcc tcccctcgga gcggatatcc gccaggatga cagcggcatt
     3061 agcgtgggga tggttggcga ggaaggacat gcctggttaa gcggagtcgc tgaaaatcaa
     3121 aagtttaccg tggtctgggg tgatagccag cattgctcgc tccatcttcc tgaacatatg
     3181 gaagacaccg caaatcgcct gattttacct tgtcattaat taaggaaatg aaaatgacat
     3241 ttatgaaagg actacccctt ttgttgttgg ttgccagtct gtgcagccac gctgcactac
     3301 aacccgatcg cactcgtatt gtgtttaacg ctaatgataa agctaccagt ctgcgggtgg
     3361 ataatcgtag cgataaactc ccctacctcg cttattcctg gctcgaaaat gaaaaagggg
     3421 agaaaagcga tgatcttctg gtcgccctgc cgccgataca acgtctggaa ccgaaagcaa
     3481 caacgcaggt acggatagta aagcaagcct cgaccacaaa attaccgggc gatcgcgaaa
     3541 cgctattttt ctacaatatg cgtgaaattc ctcccgcccc ggaaaaaaac agcgaccatg
     3601 ccgttctgca agtcgccatt cagagtcgta tcaaggtgtt ctggcgaccg gctgcattac
     3661 gcaagaaagc aggagaaaag gtcgaactgc agttacaggt cagccagcag ggcaaccaac
     3721 tgacactgaa aaatcctacc gcgtattatc tgaccattgc ctacctggga cgaaatgaaa
     3781 aaggcgttct ccccggcttc aaaaccgtga tggttgcgcc ttttagtacg gtcaacacaa
     3841 acaccggaaa ttatagcggc agccagtttt atctcggtta tatggatgat tacggcgcgt
     3901 tacgcatgac gacgctcaac tgcagcggac aatgccattt acaagccgtg gaggcgaaga
     3961 aatgagtgct ggcaagggag tgttgctcgt catctgtctg ttatttctgc cgttgaagtc
     4021 tgcgcttgcc ctgaactgct actttggttc atccggtggc tcggtagaaa aatcagaagc
     4081 gattcaacct tttgctgtac caggcaatgc caaactcggc gataagattt gggaatctga
     4141 cgatattaaa attcccgtct attgtgacaa caataccaac ggcaattttg aaagtgagca
     4201 tgtttatgcc tgggtaaccc cctacccggg ggtgcaggat cgctattatc aactcggcgt
     4261 aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg acactaacca
     4321 gtgtattgac agtaagaaca ttgacattta tacccctgag cagattatcg cgatgggatg
     4381 gcagaacaaa atttgctcgg gtgatcccgc taatattcac atgtcgcgta cctttctcgc
     4441 tcgcatgcgg ttatacgtca aaatacgaga aatgccgccg catgattatc aaagtacgct
     4501 tagcgactat atcgtcgtgc aatttgacgg tgccggtagc gttaacgaag accctactgc
     4561 ccaaaacctg aaatatcata ttactggtct ggaaaacatt cgcgtgctgg attgcagcgt
     4621 caatttttcc atttcaccgg aaacacaggt gattgatttt ggtaaattta atttgctgga
     4681 tattcgtcgc cacacaatgt cgaaaacatt cagtattaag acgacaaaat cacaaaatga
     4741 ccaatgcacc gacggattta aagtcagttc ctccttttat accgaagaaa cgttggttga
     4801 agaagataaa gcattgttga ttggtaatgg tctgaagcta cgtttgctgg atgaaaacgc
     4861 ctcgccttat accttcaata aatatgctga atacgccgat ttcaccagcg acatgttggt
     4921 ctacgaaaaa acctatacag ctgaactttc gtccattgca ggtaccccca tcgaggctgg
     4981 gcccttcgat acagtggtgc tttttaaaat taactataac tgatttgcca tgatgccctt
     5041 tgcagccgga aagggcatct ctttcagaaa attgctgatt ttcccctttt ccttgattgc
     5101 tgttattttt tcctggcccg gctttcttta tgctgtatcg atacaccaaa gagaaaataa
     5161 tgagggagcg aaggatgcca gttttgcagt ggggaatgtt atgtgtgcta tcacttctcc
     5221 tttctattgg tttcctcgcg gtacatctcc cggcggcgct attacttggg ccgatgatcg
     5281 ccgggatcat cttcagtatg cgcggaatta ctctgcaact tccccgctcc gcttttctcg
     5341 ccgcgcaggc cattcttggc tgcatgattg cgcaaaacct cactggttcg attctcacca
     5401 cgctggccgc caactggccg atcgtgcttg ctattttact ggtgacgctg ctttccagcg
     5461 ccatcgtggg ttggttattg gtgcgctata gctcactgcc tggaaatacc ggtgcctggg
     5521 gttcttctcc aggcggcgca gcggcaatgg tcgctatggc gcaagattac ggcgcagata
     5581 ttcgcctggt tgcatttatg caatatctgc gagtgctgtt cgtcgcgggg gccgctgttc
     5641 tggtcacccg catgatgctg ggtgataacg ctgaagcggt taaccagcaa atcgtctggt
     5701 tcccgccagt gagcattaat ctcctgctta cgattttgct cgcagtcgtt gccggtacgg
     5761 cgggatgcat gctgcgtctt ccttccggca cgatgctcat cccgatgctg gcgggcgcag
     5821 tgctccagtc tggtcagctc atcaccatcg aactaccgga atggctgctg gcgatggcgt
     5881 atatggcaat tggctggcgg attggtcttg gtttcgataa acaaatctta ctgcgggcac
     5941 tacgcccgct accgcaaatc ctgctgtcga tttttgctct gctggctatt tgtgcgggta
     6001 tggcgtgggg gctgacccgg tttatgcaca ttgattttat gactgcctac ctcgccacca
     6061 gtcccggcgg gcttgatacc gtggcggtca tcgccgcagg gagcaatgcc gatatggcgc
     6121 ttattatggc gatgcaaacc ctgcgcctgt tcagtatttt gctgacgggg ccagccattg
     6181 cgcggtttat ttcaacctat gcgccgaagc ggtcggccta gtgctggcag ccagggcacc
     6241 agtaaaacgg gcgagatgac agcgtggttt tctcaatgat gctgccacag cgttcgcacg
     6301 gttcgccatc acgatgaaaa accttaaagc gaaacagcgc cccatgatgc ttattctcat
     6361 ccacctgccc cctcgtagcg taggaaaatc gaggaatatc cagtaacgcg tgtgcgagtg
     6421 catccagttg cgccgcattg agatctttcg ctttatgatt tccagtcaac ccaacctgcc
     6481 agaggatctc cacccgcaaa taattgccaa gcccagccag aaacgcctga tcgagcagta
     6541 atcctgcaaa ctgacggtta cgaaagcgcg gtgacaataa ccgttccttt accacctccg
     6601 gcgtcagatt cggatccagc acatcgggac caacgcgttg taaaaacgga tgcgtggtca
     6661 gttgttccgg cctcaacatc tcaatatccg aggcgctata aagcagaatg gttttgtcag
     6721 ccgtttgcag ttttacccgc aatactcgcg tggtctgcgg ctcttcgccg gtatcaacca
     6781 cgcgccagac gccgtaaagc tgattatggc tgtagagcgt taagtcgttg gaaaaatgag
     6841 ttaacaacgc cttaccacgc gtttccacat gggtaacgtg ttgaccgata agttgtgatt
     6901 gataagtttt taactgcggg aaggcaaacc agacatcagt tagtggtttg cctttgatcg
     6961 ccgcctccag gttatccgct gcacggcgga tctccgggcc ttcaggcatg gtgatatcct
     7021 tttttcattc tttattaatc ggggttatgc tgcgacaaca atctcctttt cggcaaatgt
     7081 agagcgtagt cggcgggcga acgccagtgc gtgctcgccg tcgccatgca ggcagactgt
     7141 ttgcgccgtg accgttgccc attcgccggt gatacttttg actctgccgt gttgcaccat
     7201 ctctagcgtt tgcgccaacg cctgttcttc gttttcaatc aacgcgcctc gctgacttcg
     7261 cggcaccagc gagccgtcag cctgataacc gcgatcggca aacacttcct cgcgcgttgt
     7321 caggccatat tgcttgcctg cacgaatcag ctcgcttcct gccagcccaa cgagaatcaa
     7381 tgccggatcg caagcgtata ccgctctggc gatggcgtct gccagttgtg cttctttcgc
     7441 cgcctggttg tacaacatgc cgtgcggttt gacatgacgc attacgccgc cttgcgcacg
     7501 ggcaatcgtt gccagcgcgc caatttgata cagcgtctgg gcgtaaacgg tttctggcgg
     7561 cagctgcatg gcgctgcgac caaaattttc cctgtcggga aaactcgggt gagcgccaat
     7621 cgcgacacca ttttttattg cttcacgcac gcaagcctgc atgatttgcg catcgcctgc
     7681 atgaaatcca caggcaatat tggcagagga aaccagcgtt aatagctctg cgtcgctggc
     7741 gcagccttcg cccagatcgg cgttcaggtc aattttcatt gtgcagccgc cacactaatt
     7801 gttcgaaata acgttgctga tcttgccgcg ctttcagtgc ctcttccagt gaacactgga
     7861 caaaatgaat cggctgaccg agcggaattt gcgccagatg gtacatatca gcctcaatga
     7921 tacaggcaat acgcgggtaa ccatcggtgg tctgtgcgtc gttcatcaac acaatcggct
     7981 gcccgttatg tggcacctgc accacgcccg gtaacaaacc gtgagataac agttcgcgat
     8041 cggtggtgcg ttttaaaatt tgcccctgta agcgatagcc catgcggtta ctttgcgagc
     8101 taagctgcca gggcgaacgc cagaatgcat cctgcgaggc gcgatcgaac tcatgatatt
     8161 ccggccccgg caaggcgcga atgcggttgc cccacagcag ctgtttaacg ccctgcgctt
     8221 ccataaaatc acgcttcgat ttgccaatcg ggagtcggtc accatccttc agtaaacggc
     8281 cttccagccc gccaatcccc actttgagat cggtgctgca tgaccccatt accggcggaa
     8341 catcaatacc acccgcgacc gccagataac tgcgcatccc gtgctgcggg cgtttaagcg
     8401 ttaaacgctg gcctgctttc atcggcaatc gccagccggt ccagacggca ttatcatcca
     8461 gccgcgcttc gcaaccggca cccgtcagag caaaccaccc atcagtttcg aactcaaccg
     8521 ttaactgacc gagcgtgatc tccagcgcgg gggcattggc gtcattaccc accagcaggt
     8581 tagcaatgcg taacgcaggc atatccagtg cgccgcagtg gctgattccc gactggcgaa
     8641 aaccgtgacg accgccatcc tgcacagtgg tatacatgcc cgcacgaata atcttcagca
     8701 tactccctcc ttctgcggta caaagcgcac gctgtctccc ggacgtaata agatgggttc
     8761 gtcacgcgcc ggatcaaaca ggctgagtga ggtatgacca atcaactgcc agccacccgg
     8821 cgttgccagc ggataaacac cagtctgcgg cccgccgatc ccgacagaac ctgccggaac
     8881 gagcaagcgc ggttcagcgc gccgtggcgt gtgtagttgt tccggcaaac tcccgagata
     8941 cgggaagccc ggttgaaaac ctaaaaacca gaccacgtat tccacggagg agtgcaattc
     9001 aacaacctgt ttttcgctaa acccgcaatg cgccgcgacc accgccaaat ccggtccgcc
     9061 tgcaccaccg taaaccaccg gaatttcaat aaagcgagac tcaggttcca gggcctcgct
     9121 ctcctcccac cagcgttgca aacgctctat ggcatccagc gccagcgact caggattacg
     9181 caaaatcacc gtgatattgt tcatgccggg aatggcttca accacattcg gcatatccac
     9241 cagacgctgc gccagtcgcc agatccgttt ctggctagcc agcgtcaccg gcggttccag
     9301 ttccagcact accgccgttt cacctatcag ataacaacgc gctcgttgca ctttcgatcc
     9361 ctctgattat tcgttatgca ggattaggaa tatcaataaa ggtcacatca agatcggtat
     9421 tttcattcag ccactcgctc aatgcgcgaa taccaccacg ttcagtggca tggtgacctg
     9481 cagcataaaa atgcaacccc tgctcgcggg ctgaatgaat ggtctgttca gaaacttcgc
     9541 cagtaataaa agcatccacg ccaaaacgcg cggcgctatc gataaaactt tgcccgccgc
     9601 ccgtgcacca ggcgacgcgc tggaccacct caggtccggt atcgccacac cataacggct
     9661 tacgtcccag acgcgcttca atccaggagg ctagttccag tcccggcaca ggcatggtca
     9721 gctctcccca cggcaccaac ggctcaattt cgcccatgac cgtgatcccc agtaacgccg
     9781 ccagctgtgc gttattgccc agctcaggat gtacgtcaag cggcaaatgc cagccataca
     9841 ggttgatatc attcgccagc aacgttttta aacggttacg cttcattccg cgaatgaccg
     9901 gagactctcc tttccagaag tagccatgat gcacaatgac cgcatccgcg cccagacgca
     9961 ctgcctcatc gagcaaagcc tggctggcgg tgacaccggt aacaattttt tgcaccgtct
    10021 ctttgccttc tacctgcaaa ccattcggcg cgtaatcact aatcgccgcg ctgttcagtt
    10081 tttcgttaat cagttgttcc agttcggtgt ttttcatctt cgcgccttcc gtattctgat
    10141 agctacaccg ttaacatagc gttacctcgc ctgttcgtcg atagccaatc ttttgacata
    10201 ttaagaaaca aacccgacgc caaaagacgc aaattgttta tatcatcacg cttcagctaa
    10261 tgtatattta tgcaatcaga cagcatattg cataaataat cagatctgta tcaaagtttt
    10321 aactttaagg aaacacaagc acgccgcgca actattacgt gacgttgcag tcaattgtgg
    10381 aattattatg aataaacacg catcacaacc gcgcactatt tactatgtcg ttgcgctgca
    10441 aatctgggaa tattttagct tttacggcat gcgtgccctg ctgattctct atctcaccaa
    10501 tcaactaaaa tacaacgata ctcacgccta cgagttattt agcgcctact gttcgctggt
    10561 gtatgtcacg ccaatcctcg gtggcttttt ggcggataaa gttctcggca atcgcatggc
    10621 ggtgatgctg ggggcgttgt tgatggcgat cggtcatgtg gtgctgggtg ccagtgagat
    10681 ccatccgtca ttcctctatc tgtccctggc gattatcgtt tgtggctatg gtctgtttaa
    10741 atccaatgtc agttgcctgc tcggcgagct gtatgagcca accgatccgc gtcgtgatgg
    10801 cggtttctcg ctgatgtatg cagcgggtaa cgtggggtct attatcgcac ctatcgcctg
    10861 tggttacgcc caggaagagt acagctgggc gatgggcttt ggcctggcgg cggtgggtat
    10921 gatcgcgggt ctggtcattt tcttatgtgg caatcgtcat ttcacacata cccgcggcgt
    10981 taataaaaaa gtactgcgtg caacaaactt tctcctgcct aactggggat ggctgctggt
    11041 tctgctggtg gcaacgcctg cgctgattac cgtactgttc tggaaagagt ggtcggtata
    11101 cgccttaatt gtcgcgacta ttattggcct gggtgtactg gcaaaaattt atcgcaaagc
    11161 agaaaaccag aaacagcgta aggagctggg gctgattgtg acgctcacct tcttcagtat
    11221 gttgttctgg gccttcgcac aacagggcgg cagctcaatt agcctttata tcgaccgctt
    11281 cgttaaccgc gatatgtttg gttataccgt tccgactgct atgttccagt cgattaatgc
    11341 cttcgcagtt atgctatgcg gtgtgttcct ggcgtgggtg gttaaagaaa gcgtcgcagg
    11401 taatcgtacc gtgcgcatct ggggaaaatt tgctcttggc cttggcctga tgagcgccgg
    11461 attctgcatt ctgaccttaa gcgcccgctg gtccgcaatg tatggtcact cttctctgcc
    11521 actgatggtg ttaggcctcg cggtgatggg ctttgcggaa ctgtttatcg acccggttgc
    11581 catgtcgcaa attacgcgca ttgaaattcc gggcgtgacc ggcgtattaa ccggcatcta
    11641 catgctgctt tctggcgcga tcgcgaacta tctggcgggc gtgattgccg atcagacatc
    11701 gcaggcttcg tttgatgctt ccggggcgat caactactcc atcaatgcat atattgaagt
    11761 atttgatcaa attacctggg gcgcactggc gtgtgtagga ctggtactga tgatttggct
    11821 gtatcaggcg ctgaaattca gaaaccgcgc gctggcgctg gagtcataat aggccagcac
    11881 cgatgcctga tgcgacgctt gacgcgtctt atcag
//
LOCUS       DAZPBM010000124        11852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPBM010000124 DAZPBM010000000
VERSION     DAZPBM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.125"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(93..356)
                     /gene="cedA"
                     /locus_tag="ACHUZQ_003071"
     CDS             complement(93..356)
                     /gene="cedA"
                     /locus_tag="ACHUZQ_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1026702.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            539..2800
                     /gene="katE"
                     /locus_tag="ACHUZQ_003072"
     CDS             539..2800
                     /gene="katE"
                     /locus_tag="ACHUZQ_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1026703.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3058..3816)
                     /gene="chbG"
                     /locus_tag="ACHUZQ_003073"
     CDS             complement(3058..3816)
                     /gene="chbG"
                     /locus_tag="ACHUZQ_003073"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1026704.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3829..5181)
                     /gene="celF"
                     /locus_tag="ACHUZQ_003074"
                     /gene_synonym="chbF"
     CDS             complement(3829..5181)
                     /gene="celF"
                     /locus_tag="ACHUZQ_003074"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1026705.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5286..6128)
                     /gene="chbR"
                     /locus_tag="ACHUZQ_003075"
     CDS             complement(5286..6128)
                     /gene="chbR"
                     /locus_tag="ACHUZQ_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR1026706.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6136..6486)
                     /gene="chbA"
                     /locus_tag="ACHUZQ_003076"
     CDS             complement(6136..6486)
                     /gene="chbA"
                     /locus_tag="ACHUZQ_003076"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1026707.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6537..7895)
                     /gene="chbC"
                     /locus_tag="ACHUZQ_003077"
     CDS             complement(6537..7895)
                     /gene="chbC"
                     /locus_tag="ACHUZQ_003077"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR1026708.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(7980..8300)
                     /gene="chbB"
                     /locus_tag="ACHUZQ_003078"
     CDS             complement(7980..8300)
                     /gene="chbB"
                     /locus_tag="ACHUZQ_003078"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1026709.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8599..8937)
                     /gene="osmE"
                     /locus_tag="ACHUZQ_003079"
     CDS             complement(8599..8937)
                     /gene="osmE"
                     /locus_tag="ACHUZQ_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1026710.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9139..9966
                     /gene="nadE"
                     /locus_tag="ACHUZQ_003080"
     CDS             9139..9966
                     /gene="nadE"
                     /locus_tag="ACHUZQ_003080"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1026711.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10196..11083
                     /gene="cho"
                     /locus_tag="ACHUZQ_003081"
     CDS             10196..11083
                     /gene="cho"
                     /locus_tag="ACHUZQ_003081"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1026712.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11043..11618)
                     /gene="ves"
                     /locus_tag="ACHUZQ_003082"
     CDS             complement(11043..11618)
                     /gene="ves"
                     /locus_tag="ACHUZQ_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1026713.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         3134 a         3041 c         2706 g         2971 t
ORIGIN      
        1 ctttttgtta caggtggttt gaatgaatgt agcaacgaaa tgcagaattt caggtcatgt
       61 aactcccggc aaaaccggga agtatgtaat ccttactcag tcacttcccc ttcctggcgg
      121 atctgatttg cccaacgttg ggcagattca ggcacagtaa acgccggtga gcgcagaaat
      181 gactctccca tcagtacaaa cgcaacatat ttgccacgca gcatccagac atcacgaaac
      241 gaatccatct ttatcgcatg ttctggcggc gcgggttccg tgcgtgggac atagctaata
      301 atctggcggt tttgctggcg gagcggtttc ttcattactg gcttcactaa acgcatatta
      361 aaaattagaa aaactgtagt ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat
      421 ttcataacac cgtttccaga atagtctccg aagcgggatc tggctggtgg tctatagtta
      481 gagagttttt tgaccaaaac agcggccctt tcagtaataa attaaggaga cgagttcaat
      541 gtcgcaacat aacgaaaaga acctacatca gcaccagtca ccactacacg attccagcga
      601 agcgaaaccg gggatggact cactggcacc tgaggacgga tctcatcgtc cagcggctga
      661 accaacaccg ccaggtgcac aacctaccgc ccctgggagc ctgaaagccc ctgatacacg
      721 taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac
      781 cactaatcag ggcgtgcgca tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg
      841 tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg accatgagcg
      901 catcccggaa cgtattgttc atgcacgcgg atcagcagct cacggttatt tccagccata
      961 taaaagctta agcgatatca ccaaagcgga tttcctctca gatccgaaca aaatcacccc
     1021 agtatttgtt aggttctcta ccgttcaggg tggcgctggc tctgcagata ccgtgcgtga
     1081 tatccgtggt tttgccacca agttctatac tgaagagggt atttttgacc tcgttggcaa
     1141 taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg ttcatgcggt
     1201 aaaaccagaa ccgcactggg caattccaca agggcaaagt gcccacgata ctttctggga
     1261 ttatgtttct ctgcaacctg aaactctgca caacgtgatg tgggcgatgt cggatcgcgg
     1321 catcccccgc agttaccgca ccatggaagg cttcggtatt cacaccttcc gcctgattaa
     1381 tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa ccactggcag gtaaagcctc
     1441 actcgtttgg gatgaagcac aaaaactcac cggacgtgac ccggacttcc accgccgcga
     1501 gttgtgggaa gccattgaag caggcgattt tccggaatac gaactgggct tccagttgat
     1561 tcctgaagaa gatgaattca agttcgactt cgatcttctc gatccaacca aactgatccc
     1621 ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca atccggataa
     1681 cttctttgcc gaaaacgaac aagcagcatt ccatccaggt catattgttc ctggtctgga
     1741 cttcaccaac gatccgctgt tgcagggacg tttgttctcc tataccgata cacaaatcag
     1801 tcgtcttggt gggccgaatt tccatgagat tccgattaac cgtccgacct gcccttacca
     1861 caacttccag cgtgatggca tgcatcgtat ggggatcgac actaacccgg cgaattacga
     1921 accgaactcg atcaacgata actggccgcg cgaaacaccg ccgggaccga aacgcggcgg
     1981 ttttgaatca tatcaggaac gcgtggaagg aaataaagtt cgtgagcgca gtccatcgtt
     2041 tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg
     2101 ccatattgtc gatggtttca gttttgagtt aagcaaagtc gttcgtccgt atattcgtga
     2161 gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg tggcgaaaaa
     2221 tctcggtatc gaactgactg acgaccagct gaatatcacc ccacctccgg acgtcaacgg
     2281 tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg tgaaaggtcg
     2341 cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg ccattctcaa
     2401 ggccctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg gtgaagtgac
     2461 tgcggatgac ggtacggtgt tgcctatagc cgctaccttt gccggtgcac cttcgctgac
     2521 ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca acggcgatgc
     2581 caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg cgggagacgc
     2641 gcgcaagttt aaagcaacaa tcaaggtcgc tgaccagggt gaagaaggga ttgtggaagc
     2701 tgacagcgct gacggtagtt ttatggatga actgctaacg ctgatggcag cacaccgcgt
     2761 gtggtcacgc attcctaaga ttgacaaaat tcctgcctga tgggaggcgg cgcaattgcg
     2821 ccgcctcaaa tgatttacat agtgcgcttt gtttatgccg gatgcggcgt gaacgcctta
     2881 tccggcctac aaaactgtgc aaattcaata tattgcagga aacacgtagg cctgataagc
     2941 gaagcgcatc aggcagtttt gcgtttgtca gcagtctcaa gcggcgcagt tacgccgcct
     3001 ttgtaggaat taatcgccgg atgcaaggtt cacgccgcat ctggcaaaca tcctcactta
     3061 cacatcccga taactcccca accgataacc acgctgagcg atagcacctt tcaacgacgc
     3121 tgatgtcaac acatccagct ccgttaagcg tgggaaacag taagcactct gacggatagt
     3181 attatcgata aacgccggat gacacatcac ctccagcgaa cgatcacccc gatggcttgc
     3241 gtcatccagc acttgcagga acagtgcctc gctaatctct tcaccataaa acgcgctact
     3301 gaatccctga gaactgcgca gattagccgg taaatcacca gagttagata gcggctgacg
     3361 gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg gcaacaatcg ggaaaatctg
     3421 cgggaacata tgcacatgat gatggctatc aagatgcgta ggtttgcgtc caaatagctc
     3481 aatgaaacgc aaatactgac tggcaagctc ctgagtaatt tcttccagcg gtaaagcatc
     3541 ttcttctgcc aactgccaga tccattttcc cagcacacca tcgcgggtta accccggcat
     3601 agctgtcagt ggcttgccca tagtaaggac aaagtacatc cctatggcca gactcggttc
     3661 atcacgactc aactgcaccg catggtcaat agcctgccca ttcaccagcg ccgtcgtcga
     3721 tgtgacaatc ccattgcgac aggcctcgat aatgccgtag ttctgacctt tgcttaagcc
     3781 aaaatcatcg gcattaacaa tcagtaagcg ttccataatc agcctcggtt aatgtgcttt
     3841 tttaagctct gcgatgcagt cggcaaaatt tggcagccat ttctcgtgcg ccagaatcat
     3901 ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc aacggactaa ggtttagcgc
     3961 cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg ctggcagcaa tctcgaagcc
     4021 tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa tgcgtaatgc gcggatgtgg
     4081 cgtcgcgcca tcgcgcccca gcttacaggt catttctacc gcccagtctg ccggaatatt
     4141 atcaatatgc ccatgatgcg ggatattaac gtaatgttca gcttgcttgt cgttgtagat
     4201 agcgttgatc acttcgcacg ctgcatcaga gtaataagcc ccaccgcgct gttccagttc
     4261 tttcggctta actttcaact ccgggttttt atacagctca aaaagttgtt tctcgacttt
     4321 ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg cccatttcaa tagccagcat
     4381 ctctttttgc ttgaagtaat aaagcaaata agaacacggc aacagattca gagaacgaat
     4441 taagccttcg ctaaatggca gatcgaaaat atttttaacg ccagatgctt ttaactgccc
     4501 ggatgccaca ccatcaagca attcggcaaa gcgcgacttg ccatttacca gcacatcctt
     4561 aatgaacacc atatggttga ggccgaacaa atcgatagat aaatcatcgc tgtctttcag
     4621 catcagaaca tcgtgaataa acatcttcat gccgatcgga atattacaca cgccgataaa
     4681 gcgtttaaat ccggtatgac gataaacggc ttcagtaacc attcccgccg ggttagtgaa
     4741 gttaatcacc catgcattcg gacaaagttc ttcgacatct tttacgatgt caaaaatcac
     4801 cggaatggta cgcagacctt taaacagacc gcccgcgccg ttggtttcct ggccaagata
     4861 accatgactt aatggaatac gttcatccag ttcgcgcgcc ggtaattggc ctacgcgcag
     4921 ttgggtagta acgaaatcag catctttcaa tgcttcgcgg cgatccagcg ttttataaag
     4981 cttcatcggg acgccagcgt tatcaatcat ccgttggcag agttcaaaaa tgatatccag
     5041 tttctcttta ccatcttcga catccaccag ccataattcg ctgaccggca attcgtgata
     5101 acgcttaata aatccttcca gtaactccgg ggtatagctg ctcccgccac caatagtgac
     5161 gacttttaat ttctggctca taatttctcc cttcagtaca gaatactgat atctggcata
     5221 tctgcccccc cggacataaa taatccagca acaggacaga tatgtgaatt gtcaggtata
     5281 acgacttact gattaaattc agtcaatttc ttacgatagc tcttaggcgt aaaggacgtt
     5341 aatttcttaa acgttttaat aaacaaactc gggctactat aaccggcctc aaacgcaata
     5401 tccgtcacgg aatagttggt catttccagt tgttttttgg caaaattaat acggatctca
     5461 ttaataatct gcattggtgt tttgccataa tatcgttgag tcgctcgcgt caaatattcc
     5521 tgtgatttgg ctgacaacgt caccatattc tccagcgccg attcactaaa ctgctcttta
     5581 tcatgcatct tttctaccgt acttttcagc cactgcggta catcatcaat cacctgttct
     5641 tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac aaactcttcc
     5701 agccctgttt cgcggaaatt caatgaagaa atcactgttt ccacataggt gagaaaagcg
     5761 ttattggtcc ggtatacctg cgaagcgaca aagcaataag gcaacaacgg caggtaatgc
     5821 tgctcaaaaa agcgtttact gatcccaacg ttcaatatac gcgtggcacc aaactcataa
     5881 aaactttgat ggtgcgaacc taacggaata aaaacaaaat cgccccgttc cagtaacacg
     5941 cgcttaccgt taatctcctg gaaataacgc ccggttaata ccagagtaaa ttcataatag
     6001 tcgtgctgat gcagcccgct gatactctca gttttgttat agataaacac atggaagttt
     6061 ttgccattaa acaactgctg ttctcgggca gtggcaattt ccggcgcgtt aatcactggc
     6121 tgcatcatcg actccttatg ccttcagttt ttcatgaagc tcaattaatt cagtaatcag
     6181 ttcacgcgca agcatggacg tcattaaatg atcctgagcg tggaccagca ccagactcac
     6241 tttcatctta ccttcgcccg catcgccttc aatcagtttc gtctgtacca gatgcgcttc
     6301 attcaatgcc atccgtgact gatccatcat ggcttttgct gcagcaaaat cgccctgctt
     6361 cgcctgtttc agtgccgcat acgccaggct gcgcgcttgt ccggagttga tgataagccc
     6421 catcaccact tcttccagct cttcagcttc cgtttgcgta tcgggaatgt tatcgagatc
     6481 catcatacat cgttcctctc tattcttacc ggcacgatta cccgtaccgg catcgattaa
     6541 aatttcaggg cgttagcgat atcttcttcg cgctcttctt tatcaatcgc gttttgtgct
     6601 ttgttagcca ccacaacaaa gggcagataa attaacgttg cgataccaag gttgaagagt
     6661 gcaaccagca atgcggcgac gctaccgttg gtgttaaaga aggctcccag accggttggc
     6721 atggtccacg gtgcaatatt ggtcaccgga ggaatgatgc ccatgtagta cgctgcgagg
     6781 gtgattgccg ccagaatcgg ttgtaccagt acaaacggga taaacatcac cgggttcatg
     6841 ataattggca gaccaaacag aatcggttcg ttaatctgga agatgccgga cggcagcgcc
     6901 agttttgcca cctgacgata atcagcacgg cgagaggcga taaagatagc caggatcagg
     6961 cctaaagtcg caccactgcc cccaaggaaa ataaaggagt ccagcatcgg cttggcccag
     7021 atatggaagg tcttaccggc tgccagcgcc gcttcgacgg aaccatattg ctgataggtc
     7081 gcgatatttt ccagcgccca cggcgtcata atgccgttgt ccagtgcggt cagcgccagc
     7141 gcgccatgaa taccgaagaa ccagagcagt ggaacaaaga tcacataggc ccagcccacc
     7201 acgctaccca acgatgccag tggggttgag atggtatcca taatgatctg atggaagttg
     7261 gtgccccagg tattcaacgc ccaggcaata atccccatca cggaaagaat aataaagccg
     7321 ggaattaatg ccgagaagga acgcgatact gaagcaggta cgctatcggg caatttaatg
     7381 acccaattgc ggcggacaat aaaggtaaac atttctgcca ccaccaggcc aataataatc
     7441 ccggagatga tattcgctcc acctaaccag tttgcgccaa ccacataggc ctcaccgaca
     7501 ctatatgggg tgacggtcat aaatgctgca acggataaca acccagccgc cagcgcatcg
     7561 actttacgct cttctgccag cgccatgcca ataaagaacg gtgccattaa agacattatc
     7621 cctaatgttc cgttatatac gttgccgcca atacctttca ggccattaag tgtttcaatg
     7681 gttgaggcgt cgaggcgaat acctaaggaa taaaaaaacg ccccctcccc aaagcttaga
     7741 aaaacgttgt taattaatac aaacatggcc cccgcaaggg ttaacggcat taagcgaata
     7801 aagccatttt tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg
     7861 agtacctttt caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa
     7921 tttaaatatt gcggtattga tttatgaaat aactctttga cgggaaaatt taaaataaat
     7981 taatttgctg cggctttttt aatcgctgca accgcagcct taagcacgcc taaaccatcg
     8041 actttgccat aaagcagcga gtcaattact tcaaccggtt tgttgggtaa caaacgctgg
     8101 atttcgggca acatataagc aatctgcggc cctaataaca cgacatcggc attctgacct
     8161 ttttcaccag ccagtgtttc cggaaatgct tcaataatga ccggaacttc atatttttct
     8221 gcctgtgcgc gcatttttga taccagtaaa gaggtagaca tgcccgcaga acaaaacaga
     8281 taaatgtgtt tcttttccat aaaactgccc tcatcgacga ttatctgtca gccagacact
     8341 ccgcaagcct taacctgctt ccatgctctg ggtaacttgc gaaaccaaca tgactttttt
     8401 tgtggctgaa aggagtatac ggtatagacc tgagcaatag tatttctttg accttataaa
     8461 ttgtttctcc ttgacctgtg gttatgaccc tcttcacatt tcgggcaaat aattcgcgaa
     8521 gataattaag aactcaggcc aaaaaaaacc ggcgcaatgg ccggtttccg ttgttactca
     8581 agctttcaga cgaattgatt acttcgcagc ctgtggatca gtgtcgtatt cagcacaggt
     8641 ctgataaccg gagttgatga catgtccggt atcatctaac gcgacaaagt aggtttctgc
     8701 tttaccatca cgttgaccca ggatgtaggt ctggcaagta ccacgagcat ggatcatgct
     8761 cacttcagac gaaggtttac ccgcaatttg tgcaacctgc gcccggctca tgcctttttt
     8821 gacgtctttc accacaggct gtacaaactg gtctttagta cgatcataag ccgtacaacc
     8881 cgccagcatg gttaataccg ccgctgcact cagaattcct gccatattct tgttcatatt
     8941 ccgtcctctt gtttatcagc gtgttagata agcctggaat acattgggcg ctttttcaag
     9001 cccgcgaacg aaacggctcc gctttcagag gattcctgta tgacgtttta accaccattc
     9061 agcccgctgt cgcttgtcgt ttcagtggca acgggttagc tttaaggaag ttttgtcttt
     9121 tctgtctgga ggggttcaat gacattgcaa caacaaataa taaaggcgct gggcgcaaaa
     9181 ccgcagatta atgctgaaga ggaaattcgt cgtagtatcg attttctgaa aagctacctg
     9241 caaacttatc cgttcattaa atcactggtg ctcgggatca gcggcggtca ggactccacg
     9301 cttgccggaa agctgtgcca gatggcgatt aatgagctgc gcctggaaac cggcaacgaa
     9361 tcactgcaat ttattgccgt acgcctgccc tatggtgttc aggccgacga acaagattgc
     9421 caggatgcca ttgcctttat tcaaccggat cgcgtattaa ccgttaatat caagggcgcg
     9481 gtattggcca gcgaacaggc attgcgggaa gcagacattg aactgagcga ttttgtccgt
     9541 ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata gcattgcggg tatgaccagc
     9601 ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta ccggattctt cactaaatat
     9661 ggtgacggcg gtacagacat taatccgctg tatcgtctca acaaacgtca gggtaaacag
     9721 ttactggcgg cattaggttg cccggaacac ctttataaga aagcgccaac ggccgatctg
     9781 gaagatgatc gcccttctct gccagatgaa gtggcactcg gcgtgaccta tgacaatatc
     9841 gacgactatc tggaagggaa aaacgtacct gaacaggtcg ccagaacaat agagaactgg
     9901 tatctgaaaa ccgaacataa acgccgtccg ccaattaccg ttttcgatga tttctggaaa
     9961 aagtaataat ttgcacataa tggtttgtgc gaaaaagagt aatttgttca cgccggatgc
    10021 ggcgtgaacg ccttattcga cctacaaaac tatgcaaatt caatatattg caggagtcga
    10081 ataggcctga caggcgtagc acgtcagacg gtgtaacctt tgtcatcgac ccgcctcttt
    10141 tttaatcgct ccccgcctgt tacactggat agataaccag cattcggagt caacagtggt
    10201 acggcgttta acttctccgc ggctcgaatt tgaagctgcg gcaatttatg aatatcccga
    10261 acatttacgt tcattcctta atgacttacc cacccgacca ggggtgtatc tgtttcatgg
    10321 cgaaagtgac accatgccgc tctatatcgg caaaagcgtt aatatccgca gtcgggtgct
    10381 ttctcattta cgcaccccgg atgaagccgc catgctacgg caatcccggc gaattagctg
    10441 gatctgtacc gcaggcgaaa tcggcgctct gctccttgaa gcgcgattaa tcaaagaaca
    10501 acagccgctg tttaataaac ggttgcgccg caatcgccag ctctgtgccc tgcaattaaa
    10561 tgaaaagcgc gtcgatgtgg tgtatgccaa agaggtggat ttttcacgag cccccaacct
    10621 gtttggcctg tttgccaata ggcgcgcagc tttgcaagca ttgcagacca tcgctgatga
    10681 acaaaaactt tgttatggcc tgctgggact ggaaccgtta agtcgcggtc gtgcatgttt
    10741 tcgttcagcg ctaaaacgtt gcgccggagc atgctgcggt aaagagagcc atgaggaaca
    10801 tgcgctacgc ttgcgccagt ctctggagcg tttgcgggtg gtgtgttggc cttggcaagg
    10861 tgcggtggca ctgaaagaac agcacccgga aatgactcaa tatcatatta ttcaaaactg
    10921 gctgtggctg ggggcggtta attcgctgga agaagcgaca acgttaattc ggacacccgc
    10981 cgggtttgat cacgacggtt ataaaattct ttgtaagccg ctgctttccg gtaactatga
    11041 aattactgaa cttgatccgg cgaatgacca gcgagccagt tgatttcact gaacaacagt
    11101 ttaccttgcg gttgtaaaag ccgcagggtg tgcctgccat cgaaccagca cgcgccctgg
    11161 tcggtagtga gcaatttatc gcccagttgc cacgccccgt taatcacaaa caccaccccg
    11221 ccgcgtgagc caaaggtagt aaacgtacgt tcggcaattc tgactttcgc cttacagaca
    11281 tccaggcgtg tcatgatgtt gaaatccatc gacatctgcc cttccgtcag tttcgctttt
    11341 accacctggt ccgctgcaaa ggcaaaaggc tgtaacggtt ttaaggtatg gttaaagcgg
    11401 tctgcgcttt caaggagcat ctcgccgcct tccagcaacg tcactatcct ttccatgccg
    11461 ggaaacaaag aaaactcccc attcgccgcg atggatgcaa tgctggcacg ccagtaaaaa
    11521 tcacgttttg ccggtgggaa agtgcaaatt tcgcgcgttt caccggcagc atttcgccac
    11581 agattcaccg acattttacg catatcaaag tattccatac tcgctcccgg cccgttcttt
    11641 tcattgttat cgttcaggta accgtggatg gttatcggca actcgggtga tttacgttag
    11701 tggtgatcag gcttttttta caactgtttg agaagagaaa agaaaaccgc cgatcctgtc
    11761 caccgcatta ctgcaaggta gtggacaaga ccggcggtct taagtttttt ggctgaaaga
    11821 ttattcagca gttgcaggca ttttaccttt tg
//
LOCUS       DAZPBM010000125        11779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPBM010000125 DAZPBM010000000
VERSION     DAZPBM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.126"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHUZQ_003083"
     CDS             complement(<1..611)
                     /locus_tag="ACHUZQ_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1026714.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUZQ_003084"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUZQ_003084"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1026715.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUZQ_003085"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUZQ_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1026716.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUZQ_003086"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUZQ_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1026717.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHUZQ_003087"
     CDS             complement(5085..6716)
                     /locus_tag="ACHUZQ_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1026718.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUZQ_003088"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUZQ_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1026719.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHUZQ_003089"
     CDS             7139..7411
                     /locus_tag="ACHUZQ_003089"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1026720.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHUZQ_003090"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHUZQ_003090"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1026721.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHUZQ_003091"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHUZQ_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR1026722.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHUZQ_003092"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHUZQ_003092"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1026723.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHUZQ_003093"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHUZQ_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR1026724.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="ACHUZQ_003094"
     CDS             complement(11524..>11779)
                     /locus_tag="ACHUZQ_003094"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1026725.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2858 c         2892 g         2985 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtaa aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAZPBM010000126        11715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPBM010000126 DAZPBM010000000
VERSION     DAZPBM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.127"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUZQ_003095"
     CDS             complement(<1..242)
                     /locus_tag="ACHUZQ_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1026726.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKT"
     gene            complement(310..3761)
                     /locus_tag="ACHUZQ_003096"
                     /pseudo
     CDS             complement(310..3761)
                     /locus_tag="ACHUZQ_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515772.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(4105..4785)
                     /locus_tag="ACHUZQ_003097"
     CDS             complement(4105..4785)
                     /locus_tag="ACHUZQ_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1026727.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(4683..5426)
                     /locus_tag="ACHUZQ_003098"
     CDS             complement(4683..5426)
                     /locus_tag="ACHUZQ_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1026728.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(5437..6135)
                     /locus_tag="ACHUZQ_003099"
     CDS             complement(5437..6135)
                     /locus_tag="ACHUZQ_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1026729.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(6135..6464)
                     /locus_tag="ACHUZQ_003100"
     CDS             complement(6135..6464)
                     /locus_tag="ACHUZQ_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1026730.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(6461..9034)
                     /locus_tag="ACHUZQ_003101"
     CDS             complement(6461..9034)
                     /locus_tag="ACHUZQ_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1026731.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(9015..9428)
                     /locus_tag="ACHUZQ_003102"
     CDS             complement(9015..9428)
                     /locus_tag="ACHUZQ_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1026732.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(9455..9886)
                     /locus_tag="ACHUZQ_003103"
     CDS             complement(9455..9886)
                     /locus_tag="ACHUZQ_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1026733.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(9900..10652)
                     /locus_tag="ACHUZQ_003104"
     CDS             complement(9900..10652)
                     /locus_tag="ACHUZQ_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1026734.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(10660..11055)
                     /locus_tag="ACHUZQ_003105"
     CDS             complement(10660..11055)
                     /locus_tag="ACHUZQ_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1026735.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(11052..11627)
                     /locus_tag="ACHUZQ_003106"
     CDS             complement(11052..11627)
                     /locus_tag="ACHUZQ_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1026736.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(11642..>11715)
                     /locus_tag="ACHUZQ_003107"
     CDS             complement(11642..>11715)
                     /locus_tag="ACHUZQ_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1026737.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         2395 a         3719 c         2864 g         2737 t
ORIGIN      
        1 tgcgtttttt gctcatcctc agcattggca taactgaagg acgtaatcag ccccagcgcg
       61 tccgtaaact cataacggta tttcacgtta atcccgttca gattatcgct gccgggagcg
      121 ttcgtacggg catgaagata ccccgcgctc agtgtggact gatgttcaga cgcccatgca
      181 ggcgcaccgg atacggacag acagatggct gcggacaaaa tggctgcaca aactttacgc
      241 ataattacct ctcgcttttc tgcaataaaa aaggcgccat ttctggcgcc cgtatatggg
      301 ttataaaatt cagctgatac tgatgcctgc ggtggctttc ttcatcacca caaccagcaa
      361 atcgctgata cttgctgtgg gataccagtt attcaccagc catgctgaca ccgaaaactc
      421 cagcgtcatg tggccgcgac cagctggcat atcaataaca ccactgtaaa tcagcgtatt
      481 atccagcgcg gtacggttat aaatttcagc accgtttttc cgtactatca ggcggcatga
      541 cgaataaata tcgttattct cccgctcatg tttagcgccg ctgaatgcca ccgccggaat
      601 aacaatttgc ctgtcaaaag gctgatcgtc ataaatcctg acggtaatgg tccctgatgg
      661 ccaccgctcc ggtgcccggg agtcccgcgg aaaagcctta cccactgttt tgactatatc
      721 gccttcaatc tggttggctg acagtttccc cttaatctga cagttctcat taattgtgac
      781 attgttgagc gtcccggcgt tcgcattcac actgccactg atatctgcat ttttagcggt
      841 cagttttccg tccggtgtca gggaaaatgc cggaggatta ccgccgctgg taatggtggg
      901 agccgtcagg cgtttcagga acacgtcgtt catgaatatc tgatcgccct gaccaacaaa
      961 catcggcttt gtgttgccat tcgcaggatt aatcatcgca atcctgtctg ccgccagcag
     1021 cacctgactc tgcattcctg ctggcgtatt ctcaataccg gcaccgatac ccgcaatata
     1081 aaggcgtccg tccttcatct gttgcagctt cacagcccac atgctgttca gattattatt
     1141 tgtatcaacc tgaaccttct gtatctgctg gattgccgca ctctggtctt tcagtttctt
     1201 attgacagtc tgcgtgattt cattgctgac atccgtaatg gacgtcctga tttcagccag
     1261 gtcaggcgca agctgaccgt tatcaatctg cgtccacagc tcctgagcca gatgtgtttt
     1321 ccctatctct cctttgaaaa aatccaagta accttccgca tcatcgctcg cccgaccgac
     1381 agcctccacg aatgccgatt tgccaacggt gttcacactg cgaacgtaaa aataataatc
     1441 atggcccggt ttgatattga tactggcggc tatccagtac agcgccgtgc caagatagcg
     1501 ggcgctggtt tcaacctgcc tgatatccgc aatccgcttt tccgagaacc agaactcaaa
     1561 ctgtaccgtc gggtcataaa cggcaagatg cggcgttgcg gttatctgaa aatagcccgg
     1621 cgtcagctca atccgcgacg gtgctgccgg tgcggcaatc cggaaggtgg tggtggcagg
     1681 ttcaccctgc tggccatagc tgtttatcgc ccgcaccgtc agggtgtatt ccccgagcgg
     1741 caggccgctg aaacggtgct ccgtgtctgc ggtgatggcg gtggtcacca gtctggcatc
     1801 cgttccctta ccactggtca ggcgcagact gaagcgcaca cccttcacca cccgcggcgt
     1861 gtcccatttc gcctgcgcca gatactggcc gtcaggtgct gcactgccgg tgggatgacg
     1921 ctgttcaggg aacctgactg cggctcaaag cgggcaccgt tatccacgat ggcttctttt
     1981 tccagtacgt gctgcaccgc cgtgatggca aaggtgccgt ccgtgttttc ccggacggag
     2041 acacagcgga acaggagacg gcgcagtgac ggcagggaga gtccccacac cccgtatgtc
     2101 tccacaccat caggcagggt actgacctgt atccggtccg gcgcggggtg ttcggtgatg
     2161 tccacactca ccggcttacc gctgccgtta atcaggttca ccgtggcggc accggtctcc
     2221 ggaagtgtca cttcacggtc cagcgtcaga gtgcgggtgg cagcatcaat ggacaggaca
     2281 cgtccgccgg tcagggtccc ggcatagtcg ttatcacaga tttcaatgat gtcaccgggt
     2341 gtgtgccgca gcccctgaga cccgagcgtg aaatccaccg tctgcgtttc cagcagttcg
     2401 gtctttatca cccacagtcc ggcacggtgg gcctgaccgc ggctggtaca gccgaacgcg
     2461 tccatcttca gcaggttgcg tccgtagcgc agtatggctt ccgggtcttc caccagttcc
     2521 gtggaggtct gccagccgtt ctgcgggtcg gtataattca cctccaccgc cgtgtgccgg
     2581 tccttcaggg cactgaagct gtagcggaaa cccacgccgt tatcatccac caccacatcg
     2641 ctgttggtgt acggccacac cacatccgac gggcggtcct gaacaaacgt cagcgtctgg
     2701 ccgttccata ccggcataca gcgcatcgcc gagcagaaat cactgagcac gtcccatgcc
     2761 ttacgctgtt gtgccaggta cgcattgaag gtcatccgcg gctcggtctc cccgaaacta
     2821 tccgggaccg tctggtcgca gtactgcccg atggcataca gcgcccactt gtccacatcc
     2881 gccgccccca gacgttttcc catgccgtag cgcgggtgag tcagcatgtc ccacagacac
     2941 caggccggat tgttgctgta tgccggtttc aggctaccgt cccagatacc actgtacgtg
     3001 cgtttttccg ggtcatagtt tgacggcacc tggatgatgc gaccgcggat atggtagttc
     3061 accgtcatct gctggccgcc gaactgctcc gcatccacct gcagccccac aatggccgtg
     3121 ttcgggtagc actgtttcac atcgatgatt tcggtgtatg acgaccacag cgtcttattc
     3181 tgcagctggt ccgtggtgct gtccgctgtc tcccggacca tccggatgtt aaaaggacgg
     3241 ggaggcagat tatccagaat caccgacgcc agaaactgcg aggtggtctt gccgttaatg
     3301 gtgacatcct tttctgtcac ccagttaccg ttacgctgta actgaatcag caggcggacg
     3361 gatgtcgggt ttcggtcacc ctttgacgtg gtctgcacca gtgactgcac cccgaaggta
     3421 acccgcaggc ggtcaatgtt cgcggacgta atggtgcgcg tcaccggttt tgccttcgtc
     3481 acttccacgc ccagtccggt ttcagcaccg gaggactcaa agccttccgg tggtgtctgc
     3541 tcctgctccc cggcgcgtca gaccgccgtc acaccgtgta tcacgggatt accgtccgtg
     3601 tccgtcagcg gggttttgtt caccaggata ctctgcagtc ccttcaccgg accttctatc
     3661 agtccctcac caatcgcatc aatcacgctc atcatctgcg tggatttgag attatccttc
     3721 gcctcacgag gcgtgtgtgc cttaccgcca ccttttccca tacagccttc ccctgaataa
     3781 attaaccgcc acttgccatt ccgtacagaa gtcggatatc cttcgcccga aaagcatgaa
     3841 acacatttct gccatgctaa agagaaaccc cggtatcagc agataccggg gttttctttc
     3901 atgcccaccg ataatcctgt tggttaaaac cggtaatggc ataaaaattc tgaatatctt
     3961 cacattttca caaactgact gtggcgcgta taatttctct gcgttaattt ttttgtcgtg
     4021 atataagaat aattccttac acttaatctt cgtaactctc ccgcagttcc tgtccgcgat
     4081 cactgcggga tttttttatt ctttttaccc ctgccgcccg ataaccacga cctttccgcc
     4141 cccgccttca tcacgggtgc tgatgtcctg ggatatacgg cgggagccaa ccagcatttc
     4201 cccgtaaggc accggcatcg ggttcccctg ggcaatcatg ttatccagtg aggaaaagta
     4261 cgtgttctgt ctgccgttat ccgttgcgcg gtaatccggt gtttttgcct tcggggccag
     4321 catctgggcc acaccgccca gaatcatgct ggctccaagt gaaaacagca tcgtggtggc
     4381 agaaaaacca ccggctgcca gggctgaacc ccataacgcc attgatgccc cggcagtgaa
     4441 gaaagagccc acgatggctg ccgcccccag cacaatctgc agtccaccct ttccggcccc
     4501 ggccagtcgc ggcacaatgt ggatgaccgt tccctcaccc agctgttcgt gaagacgggc
     4561 gtacaccgcc tccggtgccg tgtcataacc ggcaatacgt atctggtacc agccttcgtt
     4621 catctgacgg cgaaagcccg gcatctgcat cgacagggcg cgaatggctt ccgctgccgt
     4681 gttcacatac aggctgaggc ggcggccaaa tcgttgtaaa tccccgtgaa ggcagatgcg
     4741 tgccagtggc ggtgacgcca gactgaatgc gttcgtcgtt gccatttttc ggaatacctc
     4801 tcccgtttac tcagttgttc aggcagatgg tgaagcagct caccgttgcc gcagtaaatg
     4861 gcggcatggt tcggtaccga agcaccaaag cagcacagca gaatatcgcc cgcctgtgca
     4921 gaggacaggg gcacccggta aaagccggtg accgccatat tgtccaggta aaggttctga
     4981 ccgttgcgcc accagtcatc ctcgcgatga aaatccggca tttcagtccc cgccagatga
     5041 taagcatccc ggaacagcgt gtaacagtcc gtcaccccgt gctcaaagcg ccgtcctgtc
     5101 agatgtggca cacagcggaa tttgtgaatg tcaccccggc agaccaacca ccagggcagt
     5161 gcgcttttta tctgcaaccg ccggtcagcc tcgctcagcc agggcagccc accgggatga
     5221 ctgtggacca gtgccacaat ctccccctgc atctctgccc gcagccagtc ttccggtgcg
     5281 atacgaaaat acgcctccgg ctctgcggaa atattcacac aagggatata ccgctccccc
     5341 tccggcgtgc ttatcacgaa gccgcacgac tccgcaggcg cacaccgccg ggcatgtgcc
     5401 agaatcgctg attcagtctg tgtcataaac cgggatttac tgcgaaagtt tattaatgga
     5461 aaggaaaccg ccaaaattgc cgacattcct gcgcagttca cacccgcgca tgcacttgct
     5521 gcatctgtcc ttacggatat ccgtggtggg gttgtcgaac tcatccgcca ccgcaggacc
     5581 gttatacccg cattcatctc cccggtaatc ccacatacag gtgttcgcca gcatgatgcg
     5641 accgggaaac agcgccccgt ccgtctcggt cggtgtagcc agcacaaacg aggccgtcat
     5701 ggctgtcagc tgcgacatct gctccaccac ccagcggtca ctcagctcct gctccgggtc
     5761 cgcctccgga ttgcccgcaa cgaaattcac cgcatccaga aaacgggcat acacccggcg
     5821 gcggaccacc gtggccccca ccagactctg caggtcttcc gccatcccgg tgaccagacc
     5881 gaacagattg gacaccgtca gcgacggtct ggcactgctg ccccggccgt tcatctcaaa
     5941 gccgctgccg tcaatcgggt atgcctcata cttacgcccc tgccaggtga ccggctcccc
     6001 tttttcattc agctcattac agaaaaaata ccgctcacca ccctgtaccg tcaggtcgat
     6061 ttcccagagt accacccgcg gtgactgctc tgacttaacc gactcgttca gactttcttc
     6121 gcgaatatcc tgcatcagtt caccacctgc ttaaactccg cgctgaactc aacgcgcaac
     6181 atcccgaccc gcgcagacca cccggcacag gtcaccttta tctgccggta tgcatagggt
     6241 ggcttccaca aaaatgcctt ccagccaccg tgctctgcca ggaacgcttc cagatgccgg
     6301 gcctcctccc gggtcacgga aagcgtcacc ctgtatgttt tcaggtcagc attcagccct
     6361 gccgccatac gctgtgagta cccgtcacca aaacgcactt cacgcaccga tggctgcgag
     6421 ttcacctcca tatccggctt cactttccag cgaaaggttt tcatccaccg ctccctgata
     6481 acataccgcc atcacgcaac tgcagccgga gttcatcctg tgcccccttg cgggccatct
     6541 catacaccgc tttcatcagc tgcggccctg cccgcccgtt ggggccgtcg ttctgaatca
     6601 ccacgtgatt gttctgatta aaattaatgc cttccgcccg ccgcatctgc gccggacttc
     6661 cggcaccgcc gacataacca ccttccgcat agccccgcat caggcggtac agattgccga
     6721 caccaatccg gctggtcgcc tccttcgtga agacaaactc cccgcgatga acaatccccg
     6781 caggttcata tttacccccc gtccccgtaa atcccccggt cgcgaaatgg aagttcgccg
     6841 ccgcagcctg aatggccgtc cccgtggagg cagacgcacc accaccgaaa gcaccgccaa
     6901 tggcgctgcc gatactcccg actatcccca ccatcgcctg cttcagaaaa atctctgtca
     6961 gcatggacag cacagaacgg gtgaaaccac gccagttctg ttcgctgccg atcagcatcg
     7021 ctgccatatt ctgtgcaata ccgtcaaagg tctgcgtggc cgcgttttta acctgcgaaa
     7081 aactgtccgt cgcactttcc gcccactcgc cccagccgga cttcatcccg gccatccagc
     7141 ttccacgaag ctgctcctcc gcagaccagg tgttcttcag tgcagatgtg gccttcgcca
     7201 gcgcagccgg attatcaccg tacacctcac gaaggcgctg ctcttccgac tcccgctgcg
     7261 cctgacggtc ggtgagtccg cgggcttttg cgctgattgc cgcctgcttc gcgctctgct
     7321 gctgttcaaa ccgcgccgcc tgctgtgcca gctcattcag ccgtttctgg tgttcaatct
     7381 tgtcgcccag ctcagccagc tggcgtttgt actccagcgt ttctttctca tgggccagca
     7441 gggatttttc ctgctcagat aactgccgtt tcgtggcggc ctctttcagg accacatact
     7501 gattttccgc tttccataaa tcacggcgct gctggctgat tttttcattc gcaccgctgt
     7561 gcttctccag cgtcctgagc tcagtttcaa gcgccagcag ggctgcatgc gcctggtctt
     7621 cctggcgctc accggcagac accttcacac cggacggctt tttctgcgtc gactcataat
     7681 ccttttttgc cgacgccatc agcgtgttgt aatccgcctg caggattttc ccgtctttca
     7741 gggccttatt cagctcttcc tgccgggcgg tatatttctc cagtggcgtc agcaggcgct
     7801 catacgcctt ctgcgcctct ccggtatact tcagctgtga cgactcacgc tcagccctgt
     7861 cccttgccgc cagttcaccg gctttttcca tatccgactg cagcgttgcc gctgccagac
     7921 ccagacgggc attttcccgg tcatcccatg cgccctgaag gttggcccgg aaagaggagg
     7981 ttttaccgcg gcgctggctc cgactctggt accactgcca ttttttatcc gcctcatcaa
     8041 atgccttctg cgcactggcg agcatatccg ctgaggattc cggacgaccg atatccagaa
     8101 tggcatccca catcgatttg aatgccttcc ctgttttatc cgcccaggtc tccagtgttc
     8161 ccatgttttc tttcaggcga cgggtctgct catcaaagcc tttcgtggcg atatcgttcg
     8221 ccgcctgcaa tgccccggcc tcgtctccgg aacgctgcag ctgtgcaaca tacgcaatct
     8281 gctctgccgt cacgttacgg aactggcgcg ccatcgccat cagtcccgac gtcgggtcag
     8341 tggtcagctt cccgaaggct tcagcgactt tatccacctc cacaccggat gcagacgcaa
     8401 aacgcgcgac actctggttg atggcatcaa actgttcacc accacgcaca ccggcattca
     8461 ccagggctgc cagtgactct ctcgcctggt taaacgtcag ccctgctgcc tgcccggctc
     8521 ttgagagagt cagcatacga tcggcagtca gtccggactg attaccggaa agaaccaggg
     8581 ttttattaaa cgctgaaagc gtggaatctc cctggtacca ggcgtacacc agtgcacctg
     8641 tcgccaccgc cagcgaggtg accccgacca tcggcagggt gatcgcatcg gcaagccccc
     8701 tgaacatggg gatcatcccg ccgaaggagt ccttcacctg accgccctgt tgcagcagga
     8761 tcagccaggg attctgacca ccggcaagct gcgtggcgat atccgtaaac tgtgcgggca
     8821 gggttcgcat ggccgcttta tactgcccga cggaaatccc ggctttttgt gcagccagcg
     8881 cctggcggct caggccctgt tcaacagcac tggcggtttt tctggcgtcg gtatccagac
     8941 ctgaaaaatg acgccttacc cggctcatct gctcatcgaa acggacagca tccagactca
     9001 ggtcaataac aagatcacca accggctggg acatatctca cacctcccgg aatccccgct
     9061 gaagccatca ttaatgcggc atcatccacc atgacatccg ccacatccgc agacgataaa
     9121 atatcgcgcc ctccgtcccc accgaaccgg acgcctccgg caagtcctgc cgctttctgc
     9181 atcagcattt tgtcctcatc cggcctctcc acctgctctt cctcatgccg ggggacaagc
     9241 agactgaaat cagagggatg catatccgga tcgcaaaaaa acaggctgag tacagcgtac
     9301 gtcagcccgg aaaaatgcat atccagctgg gtatcctgaa aataatgcgt gcggtaaaaa
     9361 cggcgccagt cggcatattc ggtggatgtc atcccggcaa gcatggcgcg ccagtcgggt
     9421 ctccccatct cacgcgccag tctgagggca aagttcagct cgccgtcgaa gactttcccg
     9481 cagaaaaatc atcatcagtc agcgtgttat ttttcgccac ttcagtaata tcagtatccg
     9541 gacgaacagc ttcgatcatc ccggacaggc acaacaccac gtcttccgcc cgggcaatgg
     9601 catcggcagg ccaggtggtg agcacttcct gctctatctt catcacggcc tcattcattg
     9661 acggtgactg cgttttctgt ggatggttat gccacaggga catcgccacc agaaacgcgc
     9721 cggttctgac gagatcttcc acgcttacct gcaggttgcc gctggattct gcctgttctg
     9781 cacgccgttt caggagggca agatgctcga tacgctgcag cgcagacaat tcggaaagcg
     9841 tgacagacac accgttatat tcaaattgtt ctgttttcag aaacatgtat tacctccgtt
     9901 taccctgcag cgcccgcttc agtaacggtg acttcagcca ctgcggcgaa ctgaccattt
     9961 ccgctcacca cagggatctg caccttacct gtcgccacgc cgtttaccgt aattgtcata
    10021 tctttcacac taatggtggc tttcgacgga tcggcggaaa ccgctctgaa cgtcttgtcg
    10081 gttgcacttt ccggctcaaa agaaaccgtc agggtggttg ttttcccttt tgccaccgta
    10141 ccggatgtcg gcgtcacctt aatcgcactg accggcgtaa ttttgctgcg ttcttccgct
    10201 acagaaggtt tacccacgtt agtgactttc accgtgcggg tgatcacttc tttcgccgtc
    10261 acggccttac cgatactgct gacccagcca cgaaacacat ccaccgtgcc attcggaaaa
    10321 cggattttat aggcccggac atcgccgctt tcaaaccagc ctataagccc tttctggcct
    10381 tcctctcccg gtttccaggc cagcgtaaaa ctggtatcac ctgcagattt ctgcccctgc
    10441 ccggtcgcgc tccagtccgc gtcttcatca tccaggtagt tatcatcata ggattcagcc
    10501 gtcatctcgc ccggcgtcag atccttcacc ttagccagtc gctgccagtc atcgtctgac
    10561 aacgggtttg cataagcatc agccttgccg ttgtaaaccc acagagtggt accggcacct
    10621 tttaccggct ccaggggatt tggtgttgcc atatcgtcct cacatctcgt atgtaatgga
    10681 ataagtcaga tccgcagagc tccataacgc catatcgtca tcacgacgat actcatagcc
    10741 ctgcgtaacc atcgtggtaa tcagtcctgc cagtgcaggg atcgcagtca tcgccggata
    10801 aatccggctt tccatccact gatcgagctc cgaatcaggt acctgtgccg gtaaaaacac
    10861 ctcaatatgc agtgtggccc gccaggtatc tgcatccagc tcttcaccgg tatactctgc
    10921 atccgtcaga taaaccgcga tcgcaggaaa atcctcttcg tcaaaaacaa cggggcgacc
    10981 atcaaacagc gtcgccccgt gttcatgctg ctcgagtgca tccagcactg cggcacgaat
    11041 gtcagtgtgt ttcatcgttt tatcgcaatc ctcagttgtt gtttcagcgc gtatgccagt
    11101 tctttaggca ggcgttcacg ccggatacgg tcaacattct catcaaatgc ctgtttcagt
    11161 ggggccgcca tcgggatttt caccacctga atgggaaggc gattacgctt tttccttccc
    11221 ttgtcgtcat tgccctcctc atatctggcc tggggaagac gttgcataac atgccagcgc
    11281 ccattattta atcgctggat aaatgcccgc tgataacgat gctgaccggc tttgagtatg
    11341 ctgttcggac gacgccccag cattctgatc cccagcttaa tcacagggag atcaccgcgg
    11401 ttaacgataa ttctggcatt cggatttctg accgtcgccc gtttcagtct ggaccgttcc
    11461 tttaccagtt tccgtctcac cctggtttcc cgggcaacct gtgacgaaga ctgattaatc
    11521 gccgttgtgg ccacgcggtt aatggtcatt gcagaagcag ccggaatggc gtttttacga
    11581 acccggttca gattgtcaat cgcctgatca agccctttta tcgccataat tcaccctgcg
    11641 tttatcgtcg ccggttaact gcgggtggtt gcccacggtt gagccagaga taacagctgc
    11701 ccccgtcatc cggag
//
LOCUS       DAZPBM010000127        11699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPBM010000127 DAZPBM010000000
VERSION     DAZPBM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.128"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHUZQ_003108"
     CDS             197..301
                     /locus_tag="ACHUZQ_003108"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1026738.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHUZQ_003109"
     CDS             complement(350..1603)
                     /locus_tag="ACHUZQ_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1026739.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHUZQ_003110"
     CDS             1710..2603
                     /locus_tag="ACHUZQ_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1026740.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHUZQ_003111"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHUZQ_003111"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1026741.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHUZQ_003112"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHUZQ_003112"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1026742.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHUZQ_003113"
                     /pseudo
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHUZQ_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHUZQ_003114"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHUZQ_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1026743.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHUZQ_003115"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHUZQ_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1026744.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHUZQ_003116"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHUZQ_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR1026745.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHUZQ_003117"
     CDS             complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHUZQ_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR1026746.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSYTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(10807..>11699)
                     /locus_tag="ACHUZQ_003118"
     CDS             complement(10807..>11699)
                     /locus_tag="ACHUZQ_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR1026747.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2959 a         2963 c         3021 g         2756 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcggccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgctagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccacaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa atctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt ccgtaccatg
     9421 gtcgatggca tcagtccatg taaagacgga aatctgcgtt ttaacaggat tctcgagcat
     9481 cgggaaccat tctaccccca gatcccagct accttcgcgt acgcctgagt tgccgccgtt
     9541 tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg tttgttcgcc
     9601 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
     9661 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
     9721 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
     9781 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
     9841 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
     9901 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
     9961 acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    10021 agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    10081 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    10141 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac caccgcaaga
    10201 gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc
    10261 gtacaggaca tgtacagctt cattgccgta atcttttagg atccgccgaa gattatcact
    10321 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc
    10381 gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc ggatagagcg
    10441 cccgcgtaaa cacgcgcgaa cctgatgatt accgtagacg tcgtcacctg tcgtatcata
    10501 ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc ggctcccgca
    10561 gttaacggtg taggaactcc agaccgcttt ctcttctacc ggagcctctg ccgcccgaac
    10621 catttgggaa aatggcagag tgaaagcact gcttgccagc gcaaggctgc caagtgcgga
    10681 ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg tggtatggat
    10741 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    10801 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    10861 tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccatggtcga ttttatcgcc
    10921 agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag ggagaatgcg
    10981 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    11041 gccggtggca atcccacgtt tctgtgcatc tatagggttg atccacactt cctgacggca
    11101 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    11161 accaaacagt tgcagtggga aggtactacg ttcaggggag tcccagcctt caaaggttga
    11221 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    11281 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    11341 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat ctttacgctt
    11401 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    11461 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga acttttgata
    11521 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc
    11581 gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac cggcgtagtc
    11641 gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa tgtcggcat
//
LOCUS       DAZPBM010000128        11542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPBM010000128 DAZPBM010000000
VERSION     DAZPBM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.129"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHUZQ_003119"
     CDS             460..1386
                     /locus_tag="ACHUZQ_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR1026748.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHUZQ_003120"
     CDS             complement(1336..2529)
                     /locus_tag="ACHUZQ_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1026749.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHUZQ_003121"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHUZQ_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1026750.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHUZQ_003122"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHUZQ_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1026751.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHUZQ_003123"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHUZQ_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1026752.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHUZQ_003124"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHUZQ_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1026753.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHUZQ_003125"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHUZQ_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1026754.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHUZQ_003126"
     CDS             complement(11165..11308)
                     /locus_tag="ACHUZQ_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026755.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2926 a         2896 c         2950 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAZPBM010000129        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPBM010000129 DAZPBM010000000
VERSION     DAZPBM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.130"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUZQ_003127"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUZQ_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1026756.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUZQ_003128"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUZQ_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1026757.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUZQ_003129"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUZQ_003129"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1026758.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUZQ_003130"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUZQ_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1026759.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHUZQ_003131"
     CDS             complement(3928..5214)
                     /locus_tag="ACHUZQ_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1026760.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHUZQ_003132"
     CDS             complement(5235..6026)
                     /locus_tag="ACHUZQ_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1026761.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUZQ_003133"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUZQ_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1026762.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUZQ_003134"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUZQ_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1026763.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUZQ_003135"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUZQ_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1026764.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUZQ_003136"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUZQ_003136"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1026765.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUZQ_003137"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUZQ_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1026766.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHUZQ_003138"
     CDS             complement(9882..10364)
                     /locus_tag="ACHUZQ_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1026767.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUZQ_003139"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUZQ_003139"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR1026768.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZPBM010000130        11387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPBM010000130 DAZPBM010000000
VERSION     DAZPBM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.131"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            333..1901
                     /gene="cydA"
                     /locus_tag="ACHUZQ_003140"
     CDS             333..1901
                     /gene="cydA"
                     /locus_tag="ACHUZQ_003140"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1026769.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1917..3056
                     /gene="cydB"
                     /locus_tag="ACHUZQ_003141"
     CDS             1917..3056
                     /gene="cydB"
                     /locus_tag="ACHUZQ_003141"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1026770.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3071..3184
                     /gene="cydX"
                     /locus_tag="ACHUZQ_003142"
     CDS             3071..3184
                     /gene="cydX"
                     /locus_tag="ACHUZQ_003142"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1026771.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3184..3477
                     /gene="ybgE"
                     /locus_tag="ACHUZQ_003143"
     CDS             3184..3477
                     /gene="ybgE"
                     /locus_tag="ACHUZQ_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1026772.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3627..4031
                     /gene="ybgC"
                     /locus_tag="ACHUZQ_003144"
     CDS             3627..4031
                     /gene="ybgC"
                     /locus_tag="ACHUZQ_003144"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1026773.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4028..4720
                     /gene="tolQ"
                     /locus_tag="ACHUZQ_003145"
     CDS             4028..4720
                     /gene="tolQ"
                     /locus_tag="ACHUZQ_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1026774.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4724..5152
                     /gene="tolR"
                     /locus_tag="ACHUZQ_003146"
     CDS             4724..5152
                     /gene="tolR"
                     /locus_tag="ACHUZQ_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1026775.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5217..6449
                     /gene="tolA"
                     /locus_tag="ACHUZQ_003147"
     CDS             5217..6449
                     /gene="tolA"
                     /locus_tag="ACHUZQ_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1026776.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            6582..7874
                     /gene="tolB"
                     /locus_tag="ACHUZQ_003148"
     CDS             6582..7874
                     /gene="tolB"
                     /locus_tag="ACHUZQ_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1026777.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7909..8430
                     /gene="pal"
                     /locus_tag="ACHUZQ_003149"
     CDS             7909..8430
                     /gene="pal"
                     /locus_tag="ACHUZQ_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1026778.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8440..9231
                     /gene="cpoB"
                     /locus_tag="ACHUZQ_003150"
     CDS             8440..9231
                     /gene="cpoB"
                     /locus_tag="ACHUZQ_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1026779.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9396..9471
                     /locus_tag="ACHUZQ_003151"
     tRNA            9396..9471
                     /locus_tag="ACHUZQ_003151"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9429..9431,aa:Lys,seq:ttt)
     gene            9621..9696
                     /locus_tag="ACHUZQ_003152"
     tRNA            9621..9696
                     /locus_tag="ACHUZQ_003152"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9654..9656,aa:Val,seq:tac)
     gene            9700..9775
                     /locus_tag="ACHUZQ_003153"
     tRNA            9700..9775
                     /locus_tag="ACHUZQ_003153"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9733..9735,aa:Lys,seq:ttt)
     gene            9922..9997
                     /locus_tag="ACHUZQ_003154"
     tRNA            9922..9997
                     /locus_tag="ACHUZQ_003154"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9955..9957,aa:Lys,seq:ttt)
     gene            10031..10106
                     /locus_tag="ACHUZQ_003155"
     tRNA            10031..10106
                     /locus_tag="ACHUZQ_003155"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10064..10066,aa:Lys,seq:ttt)
     gene            10131..10262
                     /locus_tag="ACHUZQ_003156"
     ncRNA           10131..10262
                     /ncRNA_class="other"
                     /locus_tag="ACHUZQ_003156"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10511..>11387
                     /gene="nadA"
                     /locus_tag="ACHUZQ_003157"
     CDS             10511..>11387
                     /gene="nadA"
                     /locus_tag="ACHUZQ_003157"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1026780.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         2815 a         2737 c         3052 g         2783 t
ORIGIN      
        1 tataagtctt ggaaattggc atcaaaaaga gataaattgt tctcgatcaa attggctgaa
       61 aggcggtaat ttagctataa attgatcacc gtcgaaaaat gcaaatttgc ttcaacaaaa
      121 acctgtttat tgtaaggatt ttgcggcgta atatatacgt gggatcaatt tgagttttta
      181 ttaacatgtt tgcaaccttt ctttacgccg tttttgtgtg cattcacatg gtatgatgaa
      241 agtgttcaaa caaatttcta ttggggcatg cgtgtgaccc tttctaacgg ggttcactct
      301 cggagtcttc atgcgatgag caaggagtca tgatgttaga tatagtcgaa ctgtcgcgct
      361 tacagtttgc cttgaccgcg atgtaccact tcctttttgt gccactgacg ctcggtatgg
      421 cgttcctgct ggccattatg gaaacggtct acgtcctctc cggcaaacag atttataaag
      481 atatgaccaa gttctggggc aagttgtttg gtatcaactt cgctctgggt gtggctaccg
      541 gtctgaccat ggagttccag ttcgggacta actggtctta ctattcccac tatgtagggg
      601 atatcttcgg tgcgccgctg gcaatcgaag gtctgatggc cttcttcctc gaatccacct
      661 ttgtaggtct gttcttcttc ggttgggatc gtctgggtaa agttcagcat atgtgtgtca
      721 cctggctggt ggcgctcggt tcaaacctgt ccgcactgtg gattctggtt gcgaacggct
      781 ggatgcaaaa cccaatcgcg tccgatttca actttgaaac tatgcgtatg gagatggtga
      841 gcttctccga gctggtgctt aacccggttg ctcaggtgaa attcgttcac actgtagcgt
      901 ctggttatgt gactggcgcg atgttcatcc tcggtatcag cgcatggtat atgctgaaag
      961 gtcgtgactt cgccttcgct aaacgctcct ttgctatcgc tgccagcttc ggtatggctg
     1021 ctgttctgtc tgttattgtt ctgggtgatg aatccggcta cgaaatgggc gacgtgcaga
     1081 aaaccaaact ggctgctatt gaagccgagt gggaaacgca acctgcgcct gctgccttta
     1141 ctctgttcgg cattcctgat caggaagagg agacgaacaa atttgcgatt cagatccctt
     1201 acgcactggg catcattgca acgcgttccg tggatacccc ggttatcggc ctgaaagagc
     1261 tgatggtgca gcatgaagaa cgcattcgta acgggatgaa ggcgtactct ctgctcgaac
     1321 aactgcgttc tggttctacc gaccaggcgg ttcgtgacca gttcaatagc atgaagaaag
     1381 acctcggtta cggtctgctg ctgaaacgct atacgccaaa cgtggctgat gcgactgaag
     1441 cgcagattca acaggcaacc aaagactcca tcccgcgtgt agcgccgctg tactttgcgt
     1501 tccgtatcat ggtggcgtgt ggcttcctgc ttctggcaat catcgcgctc tctttctgga
     1561 gtgtcatccg caaccgcatt ggcgagaaaa aatggcttct gcgcgccgcg ctgtacggta
     1621 ttccgctgcc gtggattgct gtagaagcgg gctggttcgt ggctgaatat ggccgccaac
     1681 cgtgggctat cggtgaagtg ctgccgacag ctgtggcgaa ctcgtcactg accgcaggcg
     1741 atctcatctt ctcaatggtg ctgatttgcg gcctgtatac cctgttcctg gtggcagaat
     1801 tgttcttaat gttcaagttt gcacgcctcg gcccaagcag cctgaaaacc ggtcgctatc
     1861 actttgagca gtcttccacg actactcagc cggcacgcta agacaggagt cgtcaaatga
     1921 tcgattatga agtattgcgt tttatctggt ggctgctggt tggcgttctg ctgattggtt
     1981 ttgcagtcac tgacggtttc gacatggggg tgggcatgct cacccgtttc ctcggtcgta
     2041 acgacaccga gcgtcgaatt atgattaact ccattgcacc acactgggac ggtaaccagg
     2101 tttggctgat caccgcgggc ggcgcactct ttgctgcctg gccgatggtc tatgccgctg
     2161 cgttctccgg cttctatgtg gcgatgatcc tcgtgctggc gtctttgttc ttccgtccgg
     2221 tcggttttga ctaccgctcc aagattgaag aaacccgctg gcgtaacatg tgggactggg
     2281 gcatcttcat tggtagtttc gttccgccgc tggtaattgg tgtagcgttc ggtaacctgt
     2341 tgcagggcgt accgttcaac gttgatgaat atctgcgtct gtactacacc ggtaacttct
     2401 tccagttgct taacccgttc ggcctgctgg caggcgtggt gagcgtaggg atgatcatta
     2461 ctcagggcgc aacctatctg caaatgcgta ccgtgggcga actgcacctg cgtacccgtg
     2521 caacggctca ggtggctgcg ctggtgacac tggtctgttt cgcactggct ggcgtatggg
     2581 tgatgtacgg tatcgatggt tatgtcgtga aatcgacaat ggaccattac gcagcctcta
     2641 acccactgaa taaagaagtg gttcgtgaag ctggcgcatg gctggttaac ttcaacaaca
     2701 cgccaattct gtgggctatt ccggcactgg gtgtggttct tccgctgctg accatcctga
     2761 ctgcacgtat ggataaagcc gcgtgggcgt ttgtgttctc ctccctgacg ctggcctgca
     2821 tcatcctgac agccggtatc gcaatgttcc cgtttgtgat gccgtccagc accatgatga
     2881 acgcaagtct gacaatgtgg gatgcaactt ccagccagct gacgcttaac gtcatgacct
     2941 gggttgcggt ggttctggta ccgatcattc tgctctacac cgcttggtgt tactggaaaa
     3001 tgttcggtcg tatcaccaaa gaagatattg aacgtaacac ccactctctg tactaagtaa
     3061 ggagctaaaa atgtggtatt tcgcatggat tctgggaacg cttcttgcct gttcgtttgg
     3121 ggtaatcacc gcgctggcgc ttgaacacgt cgaatcaggc aaagccggtc aagaagacat
     3181 ctgatgagta agattatcgc gaccttgtat gcggtaatgg acaagcgccc cctgcgggcg
     3241 ctttccttcg tgatggcgct tctgttagca ggatgtatgt tttgggaccc atctcgtttt
     3301 gccgcgaaga ccagtgaact ggaaatctgg catggtttat tgctgatgtg ggccgtctgt
     3361 gctggtgtga ttcacggcgt gggctttcgt ccgcagaagg ttctttggca agggattttt
     3421 tgcccattgc ttgccgatat tgttctcatt gtcgggctga ttttcttctt cttttaaatc
     3481 agaattctct ttaaaaaatt atgggccgct ccaggcccat aaatttttac gctcccttaa
     3541 cttgccctca ttcccaaacc tcaatcgcgc gcgtatagta gcagcgttta aaagctctaa
     3601 cttttgttgc attaccggga tgtaaagtga atacaacgct gtttcgatgg ccggttcgcg
     3661 tctactatga agataccgat gccggtggtg tggtgtacca cgccagttac gtcgcttttt
     3721 atgaaagagc acgcacagag atgctgcgtc atcatcactt cagtcagcag gcgctgatgg
     3781 ctgaacgcgt tgcctttgtg gtacgtaaaa tgacggtgga atattacgca cctgcgcggc
     3841 tcgacgatat gctcgaaata cagactgaaa taacatcaat gcgtggcacc tctttggttt
     3901 tcacgcaacg tattgtcaac gccgagaata ctttgctgaa tgaagcagag gttctggttg
     3961 tttgcgttga cccactcaaa atgaagcctc gtgcgcttcc caagtctatt gtcgcggagt
     4021 ttaagcagtg actgacatga atatccttga tttgttcctg aaggctagcc ttctggttaa
     4081 acttatcatg ttgattttga ttggtttttc aatcgcatcc tgggccatta ttatccagcg
     4141 gacccgtatt cttaacgcag cggcgcgcga agccgaagcg tttgaagata aattctggtc
     4201 tggaatcgaa ctctctcgcc tctatcaaga gagtcagggg aaacgggata atctgactgg
     4261 ttcggaacaa atattttaca gcgggttcaa agagtttgtg cgcctgcatc gtgccaatag
     4321 ccatgcgccg gaagccgtag tggaaggggc gtcgcgtgct atgcgtatct ccatgaaccg
     4381 tgaacttgaa aatctggaaa cgcacattcc gttcctcggt acggttggct ccatcagccc
     4441 gtatattggt ctgtttggta cggtctgggg gatcatgcac gcctttatcg ccctcggggc
     4501 ggtaaaacaa gcaacactgc aaatggttgc gcccggtatc gcagaagcgt tgattgcgac
     4561 tgcaattggt ctgttcgccg caattccggc ggtaatggct tataaccgcc tcaaccagcg
     4621 cgtaaacaaa ctggaactga attacgacaa ctttatggaa gagtttaccg cgattctgca
     4681 ccgccaggcg tttaccgtta gcgagagcaa caaggggtaa gccatggcca gagcgcgtgg
     4741 acgaggtcgt cgcgatctca agtccgaaat caacattgta ccgttgctgg acgtactgct
     4801 ggtgctgttg ctgatcttta tggcgacagc gcccatcatc acccagagcg tggaggtcga
     4861 tctgccagac gctactgaat cacaggcggt gagcagtaac gataatccgc cagtgattgt
     4921 tgaagtgtct ggtattggtc agtacaccgt ggtggttgag aaagatcgcc tggagcgttt
     4981 accaccagag caggtggtgg cggaagtgtc cagccgtttc aaggccaacc cgaaaacggt
     5041 ctttctgatc ggtggcgcaa aagatgtgcc ttacgatgaa ataattaaag cactgaactt
     5101 gttacatagt gcgggtgtga aatcggttgg tttaatgacg cagcctatct aaacatctgc
     5161 gtttcccttg cttgaaagag agcgggtaac aggcgaacag tttttggaaa ccgagagtgt
     5221 caaaggcaac cgaacaaaac gacaagctca agcgggcgat aattatttca gcagtgctgc
     5281 atgtcatctt atttgcggcg ctgctctgga gttcgttcga tgagaatata gaagcttcag
     5341 ccggaggcgg cggtggttcg tccatcgacg ctgtcatggt tgattcaggt gcggtagttg
     5401 agcagtacaa acgcatgcaa agccaggaat caagcgcgaa gcgttctgat gagcagcgca
     5461 agataaagga acagcaggct gctgaagaac tgcgtgagaa acaagcggct gaacaggaac
     5521 gcctgaagca acttgagaaa gagcggttag ctgctcagga acagaaaaag caggctgaag
     5581 aagccgcaaa acaggccgag ttaaagcaga agcaagcgga agaggcggca gcgaaagcgg
     5641 cggcagatgc taaagcgaag gccgaagcgg atgctaaagc tgcggaagaa gcagcgaaga
     5701 aagcggcggc agacgcgaag aaaaaagcag aagcagaagc cgccaaagcc gcagccgaag
     5761 cgcagaaaaa agccgaggca gccgctgcgg cactgaagaa gaaagcggaa gcggcagaag
     5821 ctgctgcagc tgaagcaaga aagaaagcgg cagcagagaa agctgcagcc gacaaaaaag
     5881 cagcagaaaa agcggctgct gataaggcag cagctgataa gaaagcagcg gcagaaaaag
     5941 ccgccgcaga caaaaaagcg gcagcggcaa aagcagcggc agaaaaagcc gccgcagaca
     6001 aaaaagcggc agcggcaaaa gcagcagctg aaaaagccgc tgcagcaaaa gctgccgcag
     6061 aggcagatga tattttcggt gagctaagct ctggtaagaa tgcaccgaaa acggggggag
     6121 gggcgaaagg gaacaatgct tcgcctgccg ggagtggtaa tactaaaaac aatggcgcat
     6181 caggggccga tatcaataac tatgccgggc agattaaatc tgctatcgaa agtaagttct
     6241 atgacgcatc gtcctatgca ggcaaaacct gtacgctgcg cataaaactg gcacccgatg
     6301 gtatgttact ggatatcaaa cctgaaggtg gcgatcccgc actttgtcag gctgcgttgg
     6361 cagcagctaa acttgcgaag atcccgaaac caccaagcca ggcagtatat gaagtgttca
     6421 aaaacgcgcc attggacttc aaaccgtaat cgcgatgttg actgttccga cggtcaacat
     6481 caggcaccgg ttgccacggg gttctggtag ttttgtgtat tttagtttgt taacattctg
     6541 ctaaattatc gtgggccatc ggtccagata agggagatat gatgaagcag gcattacgag
     6601 tagcatttgg ttttctcata ctgtgggcat cagttctgca tgctgaagtc cgcattgtga
     6661 tcgacagcgg tgtagattcc ggtcgtccta ttggtgttgt tcctttccag tgggcgggcc
     6721 ctggtgcggc acctgaagat attggcggca tcgttgctgc cgacttgcgt aacagcggta
     6781 aatttaatcc gttagatcgc gctcgtctgc cacagcagcc gggtagtgcg caggaagtac
     6841 aaccagctgc atggtccgcg ctgggtattg acgctgtagt tgtcggtcag gtcactccga
     6901 atccggatgg ttcttacaat gttgcttatc aacttgttga cactggcggc gcaccgggta
     6961 ctgtacttgc tcagaactcg tacaaagtga acaagcagtg gctgcgctat gctggtcata
     7021 ccgccagtga tgaagtgttt gaaaaactga ccggcattaa aggtgcgttc cgtacccgta
     7081 ttgcctacgt tgttcagacc aacggcggtc agttcccgta tgaactgcgc gtatctgact
     7141 atgacggtta caaccagttt gtcgttcacc gttcaccaca gccgctgatg tccccggcgt
     7201 ggtcaccaga cggttctaaa ctggcttatg tgaccttcga aagcggtcgt tccgcgctag
     7261 ttatccaaac gctggcaaac ggcgctgtac gtcaggtggc ttcattcccg cgtcacaacg
     7321 gtgcgcctgc attctcgcca gacggcagca aactggcatt cgccttgtcg aaaaccggta
     7381 gcctgaacct gtacgtaatg gatttggctt ctggtcagat ccgccaggtg actgatggtc
     7441 gcagtaacaa taccgaaccg acctggttcc cggacagcca gaacctggca tttacttctg
     7501 accaggccgg tcgtccacag gtttataaag tgaatatcaa cggcggtgcg ccacaacgta
     7561 ttacctggga aggttcgcag aaccaggatg cggatgtcag cagcgacggt aaatttatgg
     7621 taatggtcag ctccaacggt gggcagcagc acattgccaa acaagatctg gcaacgggag
     7681 gcgtacaagt tctgtcgtcc acgttcctgg atgaaacgcc aagtctggca cctaacggca
     7741 ctatggtaat ctacagctct tctcagggga tgggatccgt gctgaatttg gtttctacag
     7801 atgggcgttt caaagcgcgt cttccggcaa ctgatggaca ggtcaaattc cctgcctggt
     7861 cgccgtatct gtgataataa ttaattgaat agtaaaggaa tcattgaaat gcaactgaac
     7921 aaagtgctga aagggctgat gattgctctg cctgttatgg caattgcggc atgttcttcc
     7981 aacaagaacg ccagcaatga cggcagcgaa ggcatgctgg gtgccggcac tggtatggat
     8041 gcgaacggcg gcaacggcaa catgtcttcc gaagagcagg ctcgtctgca aatgcaacag
     8101 ctgcagcaga acaacatcgt ttacttcgat ctggacaagt acgatatccg ttctgacttc
     8161 gctcaaatgc tggatgcaca tgcaaacttc ctgcgtagca acccgtctta caaagtcacc
     8221 gtagaaggtc acgcggacga acgtggtact ccggaataca acatctccct gggtgaacgt
     8281 cgtgcgaacg ccgttaagat gtacctgcag ggtaaaggcg tttctgcaga ccagatctcc
     8341 atcgtttctt acggtaaaga aaaacctgca gtactgggtc atgacgaagc ggcatactcc
     8401 aaaaaccgtc gtgcggtact ggtttactaa gagaattgca tgagcagtaa cttcagacat
     8461 caactattga gtctgtcgtt actggttggt atagcggccc cctgggccgc ttttgctcag
     8521 gcaccaatca gtagtgtcgg ctcaggctcg gtcgaagacc gcgtcactca acttgagcgt
     8581 atttctaatg ctcacagcca gcttttaacc caactccagc aacaactttc tgataatcaa
     8641 tccgatattg attccctgcg tggtcagatt caggaaaatc agtatcaact gaatcaggtc
     8701 gtggagcggc aaaagcagat cctgttgcag atcgacagcc tcagcagcgg tggtgcagcg
     8761 gcgcaatcaa ccagcagcga tcaaagcggt gcggcggcat caacgacgcc gacagctgat
     8821 gctggtactg cgaatgctgg cgcgccggtg aaaagcggcg atgcaaacac ggattacaat
     8881 gcagctattg cgctggtgca ggataaatcc cgccaggatg acgcaatggt ggcatttcag
     8941 aatttcatca aaaattaccc tgattcaact tacctgccaa acgccaatta ttggctgggt
     9001 cagttaaact acaacaaggg taaaaaagat gatgcggcgt actattttgc ttcggtagtg
     9061 aaaaactatc cgaagtcacc aaaggctgct gatgcgatgt ttaaagtcgg cgtcatcatg
     9121 caggacaaag gtgacaccgc aaaagcgaaa gccgtatacc agcaggttat cagtaaatac
     9181 cctggtactg atggcgctaa acaggcacaa aaacgtctga acgcgatgta atgcataaca
     9241 cacgaccaga agtcgcatta tttctggtcg tgtcgtgcga atcataagca gttgagtgat
     9301 ctgcatcgaa atttttgttg cgctcaagtc tgaaatcagt aatatatgcc gccgttgcca
     9361 cgggatatca aacaaaccga aagcaacgaa aaagtgggtc gttagctcag ttggtagagc
     9421 agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc agttttagca
     9481 tcgaagacag atgttaagcg tgtaggataa cgttgcgtca gcaacggccc gtagggcgag
     9541 cgaagcgagt catcctgcac gacccaccac taattacggt gggttcggtg gaagtagttt
     9601 gtagtatcca gcgcagtatc gggtgattag ctcagctggg agagcacctc ccttacaagg
     9661 agggggtcgg cggttcgatc ccgtcatcac ccaccactcg ggtcgttagc tcagttggta
     9721 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccaatttt
     9781 aacatcaaac tcagatgtta agcgtgaagg ataacgttgc gccagcaacg gcccgtaggg
     9841 caagcgaagc gagtcatcct gcacgaccca ccaatcttaa agattggccc cgagtagaaa
     9901 tctttcaggt aacacccgta tgggtcgtta gctcagttgg tagagcagtt gacttttaat
     9961 caattggtcg caggttcgaa tcctgcacga cccaccatcc tgaatgatta aagcagcata
    10021 atcccgcaag gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc
    10081 aggttcgaat cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct
    10141 atctgagata ttcaaagatt cgaacctgca gcaggttcga gttgagcgca gcgaaacaac
    10201 ggagccgctc gcggcgacgg cccgaagggc gagcgaagcg agtcatcctg cacgacccac
    10261 caatgtaaaa aagcgcccta aaggcgcttt tttgctattc aggcatcctc aatttcactt
    10321 tgtaaacctg atgacatcgt cagagcttac tgtgcaagca actctacgtc ggtggaatta
    10381 ggcgtaattc gagtgacttt tccccaccat tcgactatct tgtttagcat ataaaacaaa
    10441 ttacgccgat agtagcgaat attacgctaa tgtcggtttt aacgttaagc ctgtaaaacg
    10501 agatggtaag atgagcgtga tgtttgatcc agacacggcg atttatcctt tccccccgaa
    10561 gccgacgccg ttaagcattg atgaaaaagc gtattaccgc gagaagataa aacgtctgct
    10621 aaaagaacgt aatgcggtga tggttgccca ctactatacc gaccccgaaa ttcaacaact
    10681 ggcagaagaa accggtggct gtatttctga ttctctggaa atggcgcgct tcggtgcaaa
    10741 gcatcccgct tctacgttgt tagtcgctgg ggtgagattt atgggagaaa ccgccaaaat
    10801 tctcagtccg gaaaaaacaa ttctgatgct gacacttcag gctgaatgtt cactggatct
    10861 cggctgccct gttgaagaat ttaacgcatt ttgcgatgcc catcccgatc atactgtcgt
    10921 cgtctacgcc aacacttctg ctgcggtaaa agcgcgcgca gattgggtgg taacttcaag
    10981 cattgccgtc gaacttattg atcatctgga tagttttggt gaaaaaatca tctgggcacc
    11041 cgacaaacat ctggggcgtt acgtgcaaaa acagacgggc gcagacattt tatgctggca
    11101 gggtgcctgt attgtgcatg atgaatttaa gactcaggcg ttaacccgct tgcaagaaga
    11161 atacccggat gctgccatac tggtgcatcc agaatcacca caagctattg tcgatatggc
    11221 ggatgcggtc ggttccacca gtcaactgat cgctgctgcg aaaacattgc cacatcagcg
    11281 gcttatcgtg gcaaccgatc ggggcatttt ctacaaaatg cagcaggcgg tgccagataa
    11341 agagttactg gaagcaccaa ccgcaggtga gggtgcaacc tgccgca
//
LOCUS       DAZPBM010000131        11286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPBM010000131 DAZPBM010000000
VERSION     DAZPBM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.132"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHUZQ_003158"
     CDS             79..213
                     /locus_tag="ACHUZQ_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1026781.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHUZQ_003159"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHUZQ_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1026782.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHUZQ_003160"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHUZQ_003160"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR1026783.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHUZQ_003161"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHUZQ_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1026784.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHUZQ_003162"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHUZQ_003162"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1026785.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAVLSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHUZQ_003163"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHUZQ_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1026786.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHUZQ_003164"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHUZQ_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1026787.1"
                     /translation="MKLNIRAQTAQNQHNNSPIALVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHUZQ_003165"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHUZQ_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1026788.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHUZQ_003166"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHUZQ_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1026789.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHUZQ_003167"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHUZQ_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1026790.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHUZQ_003168"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHUZQ_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1026791.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHUZQ_003169"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHUZQ_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1026792.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHUZQ_003170"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHUZQ_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2760 a         2777 c         2808 g         2941 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 caccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ctcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtg
//
LOCUS       DAZPBM010000132        11246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPBM010000132 DAZPBM010000000
VERSION     DAZPBM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.133"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1760
                     /locus_tag="ACHUZQ_003171"
     CDS             <1..1760
                     /locus_tag="ACHUZQ_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1026793.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="glaH"
                     /locus_tag="ACHUZQ_003172"
     CDS             2096..3073
                     /gene="glaH"
                     /locus_tag="ACHUZQ_003172"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1026794.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHUZQ_003173"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHUZQ_003173"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1026795.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="ACHUZQ_003174"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="ACHUZQ_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1026796.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="ACHUZQ_003175"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="ACHUZQ_003175"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR1026797.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="ACHUZQ_003176"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="ACHUZQ_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1026798.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="ACHUZQ_003177"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="ACHUZQ_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1026799.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHUZQ_003178"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHUZQ_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1026800.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="ACHUZQ_003179"
     CDS             complement(10054..10212)
                     /locus_tag="ACHUZQ_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1026801.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="ACHUZQ_003180"
     CDS             10395..10694
                     /locus_tag="ACHUZQ_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1026802.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHUZQ_003181"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHUZQ_003181"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1026803.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2786 a         2785 c         2939 g         2736 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcg
//
LOCUS       DAZPBM010000133        11244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPBM010000133 DAZPBM010000000
VERSION     DAZPBM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.134"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUZQ_003182"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUZQ_003182"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1026804.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUZQ_003183"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUZQ_003183"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1026805.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHUZQ_003184"
     CDS             complement(2698..3390)
                     /locus_tag="ACHUZQ_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR1026806.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUZQ_003185"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUZQ_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1026807.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5686..6543
                     /gene="focA"
                     /locus_tag="ACHUZQ_003186"
     CDS             5686..6543
                     /gene="focA"
                     /locus_tag="ACHUZQ_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1026808.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6598..8880
                     /gene="pflB"
                     /locus_tag="ACHUZQ_003187"
     CDS             6598..8880
                     /gene="pflB"
                     /locus_tag="ACHUZQ_003187"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1026809.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9072..9812
                     /gene="pflA"
                     /locus_tag="ACHUZQ_003188"
     CDS             9072..9812
                     /gene="pflA"
                     /locus_tag="ACHUZQ_003188"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1026810.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9894..10484)
                     /locus_tag="ACHUZQ_003189"
     CDS             complement(9894..10484)
                     /locus_tag="ACHUZQ_003189"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR1026811.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            10584..>11244
                     /locus_tag="ACHUZQ_003190"
     CDS             10584..>11244
                     /locus_tag="ACHUZQ_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1026812.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         2906 a         2822 c         2629 g         2887 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc
     5461 tcccttttaa ttaactgttt tagcggagga tgcggaaaaa attcaactca tttgttaatt
     5521 tttaaaattt atttttattt ggataatcaa atatttactc cgtatctgca taaaaaccat
     5581 gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat
     5641 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
     5701 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
     5761 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
     5821 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
     5881 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
     5941 ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag tgggcgcatc
     6001 acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
     6061 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
     6121 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
     6181 tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
     6241 cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc
     6301 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
     6361 ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt ttctcacctg
     6421 accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa cattatcggt
     6481 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgaccaccat
     6541 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     6601 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     6661 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     6721 tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     6781 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     6841 atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag
     6901 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     6961 tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac
     7021 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     7081 aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac
     7141 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc
     7201 acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat ccgtctgcgc
     7261 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     7321 ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc
     7381 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     7441 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     7501 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     7561 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     7621 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     7681 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     7741 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag
     7801 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc ttgctgcgta
     7861 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     7921 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     7981 aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga gcgcatggat
     8041 cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat ccactacatg
     8101 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     8161 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     8221 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     8281 ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc tgttgacctg
     8341 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     8401 cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg taacacccca
     8461 gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac
     8521 cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     8581 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt
     8641 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     8701 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     8761 aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     8821 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     8881 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     8941 ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg actgccaaaa
     9001 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     9061 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     9121 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     9181 gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt gaagatttaa
     9241 tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     9301 ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     9361 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     9421 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg
     9481 agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc
     9541 tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     9601 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa
     9661 tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt
     9721 acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc
     9781 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     9841 gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac atcttatagt
     9901 gcatctacca tatcccgtac ctgagataat aacgactgat aatagctcgc gtgaagttct
     9961 tccccatcga ataccactgt attacacaag aaagtgacat cggcatcttc aataccaaga
    10021 taactgcaca tattttttaa ataatcgctt atattttttt cccagcccat ctggacaaat
    10081 gattctttgt ctcctccaac cagcgccacc caacgaactt tattgaatgg taatttctgc
    10141 ccatctccat aagccagccc attattccat actctgtcaa tatatccttt tagcattgcc
    10201 gggaagctgt accaccagac aggaaaaacc accactaacg tgtcatgttc aagcagctct
    10261 gaataaagct gatgaacctc tggagaataa cgtttatcca tattcttcca gtctggttca
    10321 tcttccggcg tcatcaccgg gttgaaatta cgccgatata aatcaagttc cgtcacctgt
    10381 attttccgct ccattgcccg ctgatggatc gcatcagcaa tatgtgcggt caatgaatca
    10441 tgacgaggat gggcccatac caaataaata cgttcagact gcataatttt acctccacgt
    10501 taagttctgg agcgcacttt acgcaggcgg cataatcatg taaacgtgat gattcaggat
    10561 gagttaatga aggattttca ctaatgcgga tgaatatgtc tgactttgcc actttctttg
    10621 ctgtggcccg taatcaaagc tttcgtgcag cgggcgatga gttaggctta tcctcgtccg
    10681 ccattagcca tagtattaaa acactggaac aacgtcttaa aattcgctta ttcaatcgga
    10741 caacccgaag cgtatcgtta acggaagcgg gttctaatct ctatgaacga ttgcgcccag
    10801 ctttcgacga aattcagatc atgttagatg agatgaatga ttttcgtctg acaccaacag
    10861 gaacgctcaa aatcaatgcc gcccgggtag ctgcacgtat atttttaatg cctttactgg
    10921 ttggtttcac acgtgaatat cctgatatta aagtcgagct cacaactgac gactctcttg
    10981 tcgatatcgt ccaacagggc tttgacgctg gcgttcgact tagtggcatc gtcgaaaaag
    11041 atatgatcag cgtggcgatc ggcccaccag tgaaattatg tgtagcagca acgccagagt
    11101 actttgcaag atatggcaaa ccccgccatc ctcatgatct gcttaatcat caatgtgttg
    11161 tatttcgtta tcccagcggc aaacctttcc actggcaatt tgcaaaggag caggaaattg
    11221 ctgtcgcagg gaatatcatt ctgg
//
LOCUS       DAZPBM010000134        11242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPBM010000134 DAZPBM010000000
VERSION     DAZPBM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.135"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..309
                     /locus_tag="ACHUZQ_003191"
     CDS             <1..309
                     /locus_tag="ACHUZQ_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1026813.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="ACHUZQ_003192"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="ACHUZQ_003192"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1026814.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHUZQ_003193"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHUZQ_003193"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1026815.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHUZQ_003194"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHUZQ_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1026816.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHUZQ_003195"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHUZQ_003195"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1026817.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHUZQ_003196"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHUZQ_003196"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1026818.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHUZQ_003197"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHUZQ_003197"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1026819.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHUZQ_003198"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHUZQ_003198"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1026820.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHUZQ_003199"
     CDS             complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHUZQ_003199"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1026821.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHUZQ_003200"
     CDS             complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHUZQ_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1026822.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHUZQ_003201"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHUZQ_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1026823.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..>11242
                     /locus_tag="ACHUZQ_003202"
     CDS             10354..>11242
                     /locus_tag="ACHUZQ_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR1026824.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2554 a         3090 c         2951 g         2647 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAZPBM010000135        11149 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPBM010000135 DAZPBM010000000
VERSION     DAZPBM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.136"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHUZQ_003203"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHUZQ_003203"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1026825.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHUZQ_003204"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHUZQ_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1026826.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNVQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHUZQ_003205"
     CDS             complement(3743..4162)
                     /locus_tag="ACHUZQ_003205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1026827.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHUZQ_003206"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHUZQ_003206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1026828.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHUZQ_003207"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHUZQ_003207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1026829.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHUZQ_003208"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHUZQ_003208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1026830.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHUZQ_003209"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHUZQ_003209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1026831.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHUZQ_003210"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHUZQ_003210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1026832.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHUZQ_003211"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHUZQ_003211"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1026833.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHUZQ_003212"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHUZQ_003212"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1026834.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHUZQ_003213"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHUZQ_003213"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR1026835.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2675 a         3030 c         2746 g         2698 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgaaca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    10981 ttttaataaa tgacatttac acaacaaaaa ccacccattg acatttttaa taatgtttta
    11041 acagccaatg atggttctta gcgccgattt ttagcagact gatattttca ctaatgactt
    11101 attttctgct taccaaaaaa agccacgtta tcttgttgat acaaaagag
//
LOCUS       DAZPBM010000136        11048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPBM010000136 DAZPBM010000000
VERSION     DAZPBM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.137"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..388
                     /locus_tag="ACHUZQ_003214"
     CDS             <1..388
                     /locus_tag="ACHUZQ_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1026836.1"
                     /translation="WLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAAD
                     AFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAI
                     QRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            599..922
                     /locus_tag="ACHUZQ_003215"
     CDS             599..922
                     /locus_tag="ACHUZQ_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1026837.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            919..1749
                     /gene="amiD"
                     /locus_tag="ACHUZQ_003216"
     CDS             919..1749
                     /gene="amiD"
                     /locus_tag="ACHUZQ_003216"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1026838.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1746..2759)
                     /locus_tag="ACHUZQ_003217"
     CDS             complement(1746..2759)
                     /locus_tag="ACHUZQ_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1026839.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2858..4288)
                     /locus_tag="ACHUZQ_003218"
     CDS             complement(2858..4288)
                     /locus_tag="ACHUZQ_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1026840.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4299..5300)
                     /gene="ltaE"
                     /locus_tag="ACHUZQ_003219"
     CDS             complement(4299..5300)
                     /gene="ltaE"
                     /locus_tag="ACHUZQ_003219"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1026841.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5337..7055)
                     /gene="poxB"
                     /locus_tag="ACHUZQ_003220"
     CDS             complement(5337..7055)
                     /gene="poxB"
                     /locus_tag="ACHUZQ_003220"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1026842.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7188..8156)
                     /gene="hcr"
                     /locus_tag="ACHUZQ_003221"
     CDS             complement(7188..8156)
                     /gene="hcr"
                     /locus_tag="ACHUZQ_003221"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1026843.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8168..9820)
                     /gene="hcp"
                     /locus_tag="ACHUZQ_003222"
                     /gene_synonym="priS"
     CDS             complement(8168..9820)
                     /gene="hcp"
                     /locus_tag="ACHUZQ_003222"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1026844.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9964..10863)
                     /gene="lysO"
                     /locus_tag="ACHUZQ_003223"
     CDS             complement(9964..10863)
                     /gene="lysO"
                     /locus_tag="ACHUZQ_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1026845.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2691 a         2906 c         2914 g         2537 t
ORIGIN      
        1 atggttgcca gtaaaatgga caaaagagtt aataaatgat tatttgtatg gctgtttact
       61 atctctgtac tctatctgtg caagggatat atataatatg agcgccggga ataacgtgaa
      121 ggttgctgcg gatgctttcc ttgaaattat tttctctctt aagaataaat attgcattaa
      181 actattaagc gctcaggaac gggcgtttat ttacgaattt gcaagaatga tcttcgctta
      241 tattaatgac aaaagcattg agatcttatt actttcttgt tttgcggcgt ctgaccaaaa
      301 agcaatccag aggtatatac ctcagtcaca agatggggaa gactttagga gtcatttaca
      361 atataagttg cctctttccg ctcattaatg ttgttggtgc aacataataa gacaggtaag
      421 cggtacccta taatgaaacc aatatcgggt tatgcaacgg ataatcgtaa agacttaagt
      481 gggtgttttt gacacaatga gatgatttcc taaactgatc aaaaccttct tcccaacatg
      541 gcatatcagt tcgataatgc tttagagatc gaaggcttaa caaacaagga gatcgatcat
      601 gcaattttct acaaccccaa ctctggaagg ccagaccatc gttgaatatt gcggtgtggt
      661 gaccggcgaa gcgattttag gtgccaatat tttccgtgat ttctttgccg gtatccgcga
      721 tatcgttggc ggacgttccg gtgcgtatga aaaagaactg cgtaaagcac gggagatcgc
      781 ctttgaggaa ttaggctccc aggcgcgggc gctgggggcc gatgccgtcg tcggtattga
      841 tatcgactac gaaacggtcg ggcaaaacgg cagtatgctg atggttagcg tcagcggtac
      901 ggcggtgaaa acgcgtcgat gagaagagtc ttctggctgg tcgctgccgc tctgttattg
      961 gcagggtgtg caggcgaaaa aggcattgtc gagaaagagg gatatcagct tgatacccga
     1021 cgccaggcgc aggcggcgta tccgcgcatt aaagtgctgg tgatccacta caccgcagat
     1081 gattttgata gctcgctggc gacactgacc gataagcagg tcagctcgca ttatctggtc
     1141 cctgcggtac caccgcgata caacggtaaa ccgcgcatct ggcaactggt gccggaacaa
     1201 gaactggcct ggcatgcggg gattagcgcc tggcgcgggg caacgcgcct taacgacacc
     1261 tctattggca ttgagctgga aaaccgtggc tggcaaaaat cggccggagt gaaatatttt
     1321 gccccgtttg aaccgacaca gattcaggcg cttattccac tggcgaaaga tattattgcc
     1381 cgttatcaca tcaagccgga aaacgtagtg gcacatgcgg atatcgcacc gcagcgcaaa
     1441 gacgatccgg ggccattatt tccctggcag caactggcgc agcaggggat tggtgcctgg
     1501 ccggatgcgc agcgggttaa cttttacctt gccgggcgcg cgccgcacac accggtagat
     1561 accgcgtcat tgctggagct tttggcgcgc tacggttatg acgttaaacc tgatatgaca
     1621 ccgcgcgagc agcggcgcgt gattatggca ttccagatgc atttccgccc gacgttatat
     1681 aacggcgaag cggatgcaga aactcaggcg attgccgaag cattgctgga gaaatacggg
     1741 caggattagc gcggcagttt tccgtggtcg cgtagccagg cggcagtttt ctcgatacct
     1801 tcatctaggg tgatgatcgg ctgataacct aactcttcct gcgcacgcgt aatatccagc
     1861 gtaaagtcaa aattcaactt ggagacgccg tagtgggtca gcggcggctc ttttgctgac
     1921 ttgcggccta aacgctccat gctgcgggcg atcatatcca gcatcgggta ggggacggaa
     1981 cgaatacgac agtcaatatt caactcgtcg atcagcttct gcacgatgct gcgcagtgtg
     2041 cgatgctcgc cgttggtgat attgtacaca cgcccggaag gtagcttatc gcaggcttcc
     2101 tggcttgcca gccacattgc gtgcacggta ttttcatagt aggtcatatc gaccagcgca
     2161 ctgccgccat gcggtaacag aatactgccg tagtggtgca tcatatgcgc cagacgggga
     2221 ataaagactt tatcgtgcgg tccgaacaga ctttgtgggc gcagaatagt aaagcgcgtt
     2281 tgtggattcg cctgcgaaag catattgatc acttcttcgc tggctgcttt gctgcgggca
     2341 aactcgttgg cgaagcggtg agggcgaaaa tcttctttaa tatcgcgatg gtggtgataa
     2401 tcgaagtaca gggaggggga agagatatga ataaagttac gcacacccca ggcgacagcc
     2461 cattcaccca ggcggcgagt ggcgcgaacg ttagccagat cgaaagcctg ttgtgtcccc
     2521 cagggtgagg taaagctgga gcagtgccac agcgtatcaa tgcccgcgag catcacttta
     2581 gcttgtgatg aaaccagctc ggtcagatcc gccggaacaa actctgcgcc cattttttcc
     2641 agcaatttgc ccattgcctc gttgcgaccg gtcgctcgca cgctgatgcc tttctggcat
     2701 aaaaactcta ccgcgtttcg acctaagccg ctggtggcgc cggtaaccag taccttcata
     2761 tcaatccact gttgttgaga aaatgacgtg cgcattcttc cgtgatttcc cccatgatgc
     2821 aatgggaaac atgaaagaat aacgcaggtt ttgtcgatta atctgtgctt tgttctgcca
     2881 gtctggcgat ttgttttgcc attccgcgaa aaataaacag atgcgcgggg atcatcaata
     2941 accagtaaaa cagccccggc ataccgtgcg gatgccagaa agcgcggaca tcgatagtac
     3001 gatagtcgcc tttatcttcc aggctaaaac acagtcgtcc cagccccgac gctttcatgc
     3061 caaataacaa cgtaagttgt ttttccggtt caacgacaat cactttccag ctatccaccg
     3121 catcgccagt ctgtaaatat tcgcgctccg ggcggccttt cgccagctta tgaccgatcg
     3181 cgcggtccat caacgcccgt gtctgccaca aaatattgcc aaagaaataa cgctctttac
     3241 cgccgatttg gttcactacc tgccataaag cagcaaggct ggcggacgtt ttaacggtaa
     3301 accccgcctg tttggcaaaa taaccgtact ccggtcgcca gcgggcaaag gcctgagcgt
     3361 cgtagcccca gtcgctggag ttgaccagtt tttcctcctc tttcaacgtg ctacgtaccg
     3421 cgtcatcgaa agcgatcagc cgttgtggga tgagtgcacg tagcgcggta tcatccgcca
     3481 gcagatcgtg tttcagcccc tgaatcaacg ccctggcggt ggtgggcggt acggaagtaa
     3541 tcacattgag aaaccacacc gaaatccagc gggtggggag ggggatgggg atcaaccagc
     3601 ggcgcttacc gctcaccgcc ataaaatgtt caaactgttg ctgataactg agcacctctg
     3661 gtccggcggc ttcgaagatg cggtgttcgc tggccggatg atctaacaac gccaccagat
     3721 agtgcagcaa gttttccagc gcgatgggcg tggtgcgtga acgtacccag cgtggcggcg
     3781 ttaacactgg caggttgtag accatatcgc gcatgacttc gaacgccgct gaacctgcgc
     3841 caacgataat tccggcccga agttcggtca caggtacatt cgcttcacga agaatgtccg
     3901 ccgtagcctg acgagcacgc agatgatccg actgctcatg tggcggggcc tgcaacgaac
     3961 tgagaaagat taattgctta actggtactt cacgtagcgc atcgcggacg ttgagagcca
     4021 cctggcgctc ctgagcgata aaatcgccgc cttcgcccat gctgtgcacc agaaaataga
     4081 cggtatcgat atcctgcaac agggccggaa ggttatccgg ccagctgaga tcgactttat
     4141 ggcaactgac gtttgccagt tgcagctttg caagcctgtc gacatgacgt gccgccgcca
     4201 ggatctgatg cccttgctgg ctgagtgtgc gcaccagatg ctgaccaatg tagccactgg
     4261 caccgagaac taaaatgcgt tgcggcacgt ctctctcctt aacgcgccag gaatgcgcgc
     4321 cagtgggcgg cgacttccgc cagttgttcg cgcgagacgt caagatgcgt caccaggcgg
     4381 acaatcggcg aggcgttaat cagcacgttt ctcgctttca tgtattcgcc taacgcggca
     4441 gcattttctt ccccgacgcg aacaaacagc atattagtgt cctgacgcat cacatccgcg
     4501 cctgcttcac gcagttgctc cgccatccag gcggcgttgt cgtgatcttc ctgcaaccgt
     4561 gcgacgttat ttttcagggc atacatcccg gcggcagcca gaatcccgga ctgacgcatc
     4621 ccgccaccgg ccattttccg ccagcggatg gcacgtttaa tgtaatcacg attaccgacg
     4681 agtaatgaac cgactggcgt cccaagacct ttcgacaggc aaatggtgaa cgaatcacaa
     4741 tattgcgtga tctctttcag ttcgcagccg taagccacca cggcattaaa gatgcgcgca
     4801 ccgtcaacat gcagcgccag attgcgcttg cgggtaaatt cccatgcttc tttcaggtat
     4861 tcacgcggca gcactttgcc gttgtgggtg ttttctagac tgagtaattt ggtgcgggcg
     4921 aaatggatat cgtcgggttt gattttcatc gccactttat ccagcggtag cgtgccgtcg
     4981 gcagccgcgt cgatgggttg cggttgaata ctgcccagca ccgctgcgcc accggcttca
     5041 aacagataat tatgcgcggc ctgaccgaca atatactctt cgccgcgttc gcagtgactg
     5101 agcagagcga ccaggttagc ctgggtgccg gtcggcagaa aaatggcggc ttctttaccg
     5161 gaaagctctg cggcgtagtc ctgcagagca ttaacggtag ggtcgtctcc gtaaacgtcg
     5221 tccccaaccg gggcggccat cattgcttcg agcatggcgc ggctcggtcg ggtaacggta
     5281 tcactgcgta aatcaatcat ggcatgtcct tattatgacg ggaaatgcca ccctttttac
     5341 cttagccagt tcgttttcgc cagttcgatc acttcatcgc cgcgtccgct gatgattgcg
     5401 cgcaacatat acaagctaaa tcctttggcc tgttcgagtt tgatctgcgg tggaatggct
     5461 aactcttctt tggcgaccac cacatccacc agcaccggac cgtcgatgga gaaggcgcgt
     5521 tgcagggctt catcaacttc agacgctttt tctacacgga tacccgtaat gccgcacgct
     5581 tcggcaatgc gggcaaagtt tgtgtcgtgt agttcggtgc cgtcagtcaa atagccacca
     5641 gctttcatct ccatcgccac aaagcccagc acgctgttgt taaagacgac aattttcact
     5701 ggcagtttca tctgcactac tgagaggaaa tcgcccatca acatgctaaa accgccatcg
     5761 ccgcacatgg cgaccacctg acgttctggc tctgtcgcct gcgcacccag cgcctgcggc
     5821 atggcgttag ccatcgaacc gtggttaaac gaacctaaca ggcgacgctt gccgttcatt
     5881 tttagataac gtgccgccca caccgttggc gtaccaacgt cacaggtgaa aatagcgtca
     5941 tcggcggcaa aatgactaat ttgctgcgcc agatattgcg ggtgaatggc tttctcgctc
     6001 ggtttagcta aatcgtccag ccctttgcgg gcgtcgcggt aatcttccag cgctttatcc
     6061 agaaacttgc gatcggcttt ttcttccacc aatggaagca atgcacgcag agtcgacttg
     6121 atatcgccga ccagtgccat atccaccttg ctgtgagcgc cgatgctggc tgggttgata
     6181 tcaatctgaa tgattttggc atcggtcggg tagaaggcgc ggtagggaaa ttgcgtgccg
     6241 agtagcacta acgtgtcggc gttcatcatg gtatggaaac ctgacgagaa gccgattaac
     6301 ccggtcattc caacatcata cggattatcg tattcgacat gttctttacc gcgcagggca
     6361 tgaacaatag gcgctttaat tttcccggca aactcaacta actctttatg cgcccccgcg
     6421 cagccgctgc cacacatcag ggcgatattg ctggaataac gcagcagttg cgccagtttg
     6481 cgtaactctt cttcttccgg cgtcacgact ggttgtggcg catgatacca gtgcgtggtt
     6541 gccccttctg gcgcaggttt taacgccacg tcgcctggta acacgacaac cgaaacgcca
     6601 cggttaagca ccgctttgcg catggcaatc gccagtactt gtgggatctg ctccgggctg
     6661 gaaaccagct cgcaatagtg actacattcg cggaatagct cttgtgggtg ggtttcctgg
     6721 aaatagccgc tgccaatttc gctggaggga atatgagcgg caatcgccag taccggaacg
     6781 tgattgcggt ggcaatcgaa caggccgttg attaagtgca ggttgccggg gccgcacgat
     6841 ccggcgcaga ccgccagttc tccgctaagt tgtgcttcag cgccagcggc aaaggccgcc
     6901 acttcttcgt ggcgggtgga catccactcg atggtgccca tgcgattaag actgtcacta
     6961 agaccgttca gagagtcgcc tgtgactccc cagatgcgtt tcacccctgc cgattcgagt
     7021 gttttggcga tataagctgc aaccgtttgt ttcatggttc tccatctcct gaatgtgata
     7081 acggtaacaa gtttagttca tctgacggag ggggaaggga tgggagagaa aggaggcact
     7141 aacggttaaa tagcccgatg aaaggaatat catcgggcat aaggcgatta tgcgagaacc
     7201 aaatccccct gcggatggca ggagcaggcc agtacgtaac cttcagcgat ttcggcgtcg
     7261 gtcagcgtca ttgtactgct caccgtatat tcaccggaaa ccacttttgt cttacagcag
     7321 ccgcaaacac ccgcacggca ggcagcgaca accggaacgt tattgctttc cagcgcctcc
     7381 agtagcgtgg tgccaaccgg ggcgtaaaat tctcgtgccg gttgcagttt ggtgaatttc
     7441 agaccgctgg tcgccgcttc tgctactggg gtgaagaatt tctctttaaa gaaacgcgtc
     7501 acgccgagcg ctttcacttc ctgctctacc caatccatat acggagccgg gccgcaggtc
     7561 atcacggtac gtgaagctaa gtcaggtaca cctgccagca gttcgcgagt gagacgacca
     7621 gcgataaagc cttcggtaac gttattttct gccaccagcg ttaccggata gttacgccac
     7681 tcatcggcga aaataacatc ctgcggcgta cgcacgttgt agatcacccg cacatcggcc
     7741 tgtggacggt tcttcgcaag ccagcgacgc atcgacataa tcggcgtgac gccgcagcct
     7801 gccgccagca acaggaattt atcttctgct ttatcgtcgc aggtaaattc ccccatcgcg
     7861 tccgaaagcc agagataatc accgcgtttt acatcgcgcg tcagccactg ggagccgaca
     7921 ccgtcatcaa tccgccgcac ggtcagggtg atatattcac tcacgcctgg cgtggaggaa
     7981 atggtgtaag cacgcagcgt ttccgctgag tttcgcacgc tgaccagtgc atattgcccg
     8041 gcgcgatatg ggtagtaatc gtggcaaatc agggaaatcg tccacacatc cggcgtttct
     8101 tgcgtaatgt gatgaacctg catccgccac gggcattgat tcgttggcat cgtcatcgac
     8161 aaactcctta cgcgctcaac agttgcttca tgtcttcttc aacagtggtg atagaacgca
     8221 ggccgaattt ctcgttcagc accgccagca ggtctggtgt caggaaacca ggtgcagtcg
     8281 ggccggtgac gatatttttc acgcccagag aaagcagcgt cagcagaatg acgatcgctt
     8341 tctgttcaaa ccaggagagc accagcgaca gcggcagatc gttcacacca cagcccagtt
     8401 tctctgccag agtgacagcc agaataatcg ctgagtaagc atcgttacat tgacctgcat
     8461 ctaccagacg cggcagacct tcgatatcgc caaactcaag tttgttaaag cgatatttac
     8521 cacaggcgag ggtcaggatc aggcagtcat ccggcacgct ggtggcgaaa tcggtgaagt
     8581 agtggcgctc gccgcgtgcg ccgtcacagc caccaagcag gaagatatga cgcagttttt
     8641 cacggctcac cagatcaatc agcgtatcag cagcgccaag cagcgtctgg cgaccaaaac
     8701 ccacggtgat aaggtgcgga atttcgctgt acgggaagcc tgccatctgt tgcgcctggg
     8761 tgataaccgc agagaaatca tcaccgtcca gatgacgcac gccaggccag ccaacaatgc
     8821 tgcgggtcca gatacgatcg tcataagcgc ctacggttgg gtcgatgatg cagttcgagg
     8881 tcatcacgat ggggccaggg aaacgagcga actccacttg ctgattctgc cagccgctgc
     8941 cgtagttacc gaccagatgc ttgaatttac gcagctccgg gtagccatgc gcaggcagca
     9001 tttcgccgtg ggtgtagaca ttaacgcccg tgccttcggt ctgttccagc aggttgtaga
     9061 gatctttgag atcgtgaccg gaaatcagaa tgcatttacc cgccgtcgct ttgacgttga
     9121 cctgggttgg cgtcgggtga ccgtatttac cggtttcgcc tgcatccaga atgctcatca
     9181 ctttgaagtt catctggccg atttccattg aacactcaag aagcgcgttc atatcggcag
     9241 gccaggtccc cagccacgcc atgattttat ggtactgggc ataaatatcg ttgtcgtatt
     9301 gaccgagaac atgcgcgtgt tccatatagg ccgccgcacc tttcaggcca tacaggcaca
     9361 gcagacgcag gccgagaatg ttttcgccaa tcgccgcttt atctttgtta ggggtaaatt
     9421 ctgctgcctg acgttgcagc tcgccgagat catcgctcac cagttgcagg tcagccatcg
     9481 ggttatcgac gcgcgcgttg gcatctacag ccaggcattg tgctttcagc gcctcgcgca
     9541 aggcaatcgc ttcacgagcg tagccgacaa tacgcggaga atcgaagtta acgttggtca
     9601 gggttgagaa aaaggcacgt ggcgcgaagc tgtcaacatc gtggttgatg atgccgtatt
     9661 cacacgcttt taccgcccag gcagaaagcc cttgcagcgc cgcgatgagt aaatcctgaa
     9721 ggtcagaagt ttccgccgtt ttaccacaca tcccctgcgc gtatgagcag ccgtttcctg
     9781 ccggagtacg gatagtttgt tcacattgca cacaaaacat gatcacacct tttaaagtta
     9841 tatttaatat acatgtttaa ggttaagacg cttaacgcgg ggataaaagg gatttttcat
     9901 gcaactttaa gggagattga tttagcgcaa ttttggcggc agggatctac cgccagagag
     9961 gtattacgca gagaaaaagg cgatgaggat cggcactaac aggctaagaa taaaaccgtg
    10021 aacaattgcc gccgggacca tatccagccc gccagtacgt tgaagaacgg gcagggtgaa
    10081 atccattgat gtggcaccgc ataagcccag tgcagtagag cggctgcggc gaatcagccc
    10141 agggatcaac ataatagcaa tcagttcacg ggccagatca ttaaaaaacg ccgcgctccc
    10201 gattaccgga ccaaaagatt cggtcaataa aataccggaa agagaatacc agccgaaacc
    10261 ggaggccatt gccagcgcgg tattgatggg gagatcaaga ataaaggcgt taattaaacc
    10321 accaattaat gaactgacaa ccaccaccac ggcgacaatc attccccggc gattaaggac
    10381 aatctgcttt aaggtcatgc cattattgcg caactgaata ccaacgagga aaagtagcaa
    10441 aattaacgtg tattcactgg cttcggtcgc gtgttgtaag aaagccagtc cactcagacc
    10501 aatggcaaaa ccaatcacta ctacgccgca cagttttagc gactccagcg ccatcgcaat
    10561 acgcgacggg agtttttctt gctgatggtg gttgcgccac ggcaggcctc gctccagcca
    10621 catcagggcg gcaatattac acagtaaaat aacggtaata ctgacggcag aataatgcag
    10681 aatcgccaac aggttactgg cgaggttatc gagaaacgcc agactgatac ccataaaaaa
    10741 gagaataagg taaaccatcc agcttaatag ctgattaata acttttaacg cagcttgttg
    10801 gcgaagcgga atgaggtaac ctacaatcag gggaaccaga atgattaaca gcccagaaaa
    10861 catgaaaacc cagtccttgc aaagatgaag tcgaaatgcg cgatgacaca ctactgaaag
    10921 cggaaggacg agtaaagttg caattaaaag gaaatgttat gcataaggag cagtagagta
    10981 ttcgttttca tttaaagata ttcttgcgct ttaattacaa actgcaccga tgttggtggc
    11041 gtcaaaat
//
LOCUS       DAZPBM010000137        10938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPBM010000137 DAZPBM010000000
VERSION     DAZPBM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.138"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            69..1055
                     /gene="dcyD"
                     /locus_tag="ACHUZQ_003224"
     CDS             69..1055
                     /gene="dcyD"
                     /locus_tag="ACHUZQ_003224"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1026846.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            1070..1738
                     /gene="tcyL"
                     /locus_tag="ACHUZQ_003225"
     CDS             1070..1738
                     /gene="tcyL"
                     /locus_tag="ACHUZQ_003225"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1026847.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            1735..2487
                     /gene="tcyN"
                     /locus_tag="ACHUZQ_003226"
     CDS             1735..2487
                     /gene="tcyN"
                     /locus_tag="ACHUZQ_003226"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1026848.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            2717..3439
                     /gene="sdiA"
                     /locus_tag="ACHUZQ_003227"
     CDS             2717..3439
                     /gene="sdiA"
                     /locus_tag="ACHUZQ_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR1026849.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(3506..3730)
                     /gene="yecF"
                     /locus_tag="ACHUZQ_003228"
     CDS             complement(3506..3730)
                     /gene="yecF"
                     /locus_tag="ACHUZQ_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1026850.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(3717..3893)
                     /gene="yecU"
                     /locus_tag="ACHUZQ_003229"
     CDS             complement(3717..3893)
                     /gene="yecU"
                     /locus_tag="ACHUZQ_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1026851.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            4189..4845
                     /gene="uvrY"
                     /locus_tag="ACHUZQ_003230"
     CDS             4189..4845
                     /gene="uvrY"
                     /locus_tag="ACHUZQ_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1026852.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4842..6674
                     /gene="uvrC"
                     /locus_tag="ACHUZQ_003231"
     CDS             4842..6674
                     /gene="uvrC"
                     /locus_tag="ACHUZQ_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1026853.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            6731..7279
                     /gene="pgsA"
                     /locus_tag="ACHUZQ_003232"
     CDS             6731..7279
                     /gene="pgsA"
                     /locus_tag="ACHUZQ_003232"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1026854.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            7431..7506
                     /locus_tag="ACHUZQ_003233"
     tRNA            7431..7506
                     /locus_tag="ACHUZQ_003233"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7464..7466,aa:Gly,seq:gcc)
     gene            7561..7634
                     /locus_tag="ACHUZQ_003234"
     tRNA            7561..7634
                     /locus_tag="ACHUZQ_003234"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7593..7595,aa:Cys,seq:gca)
     gene            7647..7733
                     /locus_tag="ACHUZQ_003235"
     tRNA            7647..7733
                     /locus_tag="ACHUZQ_003235"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7681..7683,aa:Leu,seq:taa)
     gene            7928..8593
                     /gene="yecA"
                     /locus_tag="ACHUZQ_003236"
     CDS             7928..8593
                     /gene="yecA"
                     /locus_tag="ACHUZQ_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1026855.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8655..9866)
                     /gene="tyrP"
                     /locus_tag="ACHUZQ_003237"
     CDS             complement(8655..9866)
                     /gene="tyrP"
                     /locus_tag="ACHUZQ_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1026856.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            10057..10296
                     /locus_tag="ACHUZQ_003238"
     CDS             10057..10296
                     /locus_tag="ACHUZQ_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1026857.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            complement(10334..10831)
                     /gene="ftnA"
                     /locus_tag="ACHUZQ_003239"
     CDS             complement(10334..10831)
                     /gene="ftnA"
                     /locus_tag="ACHUZQ_003239"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1026858.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
BASE COUNT         2809 a         2578 c         2839 g         2712 t
ORIGIN      
        1 ccttttttat ttacgcgttt tcagcgtgca taataagaaa atcacaacaa catacatcgg
       61 aggctgttat gccactgcat aatttaaccc gttttccacg gttggagttt atcggcgcgc
      121 caacgccgct cgaatatctg ccgcgctttt ctgattatct aggacgggaa attttcatca
      181 aacgggatga cgtcaccccc atggcaatgg gcggcaataa attacgtaag ctagaatttc
      241 tcgcggcaga tgctctgcgc gaaggtgccg atacgctgat tactgccggg gtgatccagt
      301 ctaaccatgt gcggcagact gccgcagttg cggcgaaact cggtctgcac tgtgtggcgc
      361 tgctggaaaa tcctattggc acaaccgcag aaaactattt aaccaacggc aatcgcttgt
      421 tgctggatct gttcaatacc cagattgaaa tgtgcgaagc actgaccgat cccaatgccc
      481 aactggaaga gctggcgacg cgagtcgaag cacaaggctt tcgcccgtat gtcattccgg
      541 ttggcggttc taatgctctt ggcgcgctag gttatgtgga gagtgcgctg gaaatcgcgc
      601 aacagtgtga aggggcggtt aatatttcgt cggtggtagt cgcatcgggc agtgccggaa
      661 ctcacgccgg actggctgtt gggctggaac acctgatgcc tgaaagcgaa ctgattggcg
      721 tgaccgtgtc gcgttccgtt gccgatcagt tgccgaaagt ggttaaccta caacaggcga
      781 ttgcgaaaga actggagctg accgcatcag cggaaattat actctgggat gactattttg
      841 cacctggcta cggcgtgccg aacgacgaag gcatggaagc agtgaaattg ctggcgcggc
      901 tggaaggcat tctgcttgat cctgtgtata ccggaaaagc gatggcgggg ctgattggct
      961 gtatcagtca gaaacgcttc aaagatgaag ggccgattct gtttattcat accggcggtg
     1021 cgcctgcgct gttcgcctat catccccacg tttagctccg ggaacgctca tgcaagaaag
     1081 tatacaactg gttattgatt ccttgccgtt cctgctgaaa ggggctgggt atacgctgca
     1141 actcagtatc ggcggcatgt tttttggctt actgctcggg tttattctcg cgctgatgcg
     1201 cctgtcgcca atctggcccg tgcgctggct ggcgcgtttt tatatctcta tttttcgtgg
     1261 tacaccgctc atcgcacaac tgtttatgat ctattacggc ctgccgcagt ttggtatcga
     1321 attagatccc attccgtcag caatgattgg tctgtcgctg aacactgccg cctatgccgc
     1381 cgaaacactg cgcgccgcca tttcttctat tgataaaggt cagtgggaag cagctgccag
     1441 tattggtatg acgccatggc agactatgcg tcgcgcgatt ttgccacagg cggcgcgggt
     1501 ggcgttgccg ccgctgtcga acagctttat cagcctggta aaggatacgt cgctggccgc
     1561 gacaatccag gtgccggagc tgttccgtca ggcgcagttg attacctcgc gcacgctgga
     1621 ggtgttcacc atgtacctgg cggcttcgct catctactgg atcatggcga cagtattatc
     1681 gacgttgcag aaccattttg agaatcaact taatcgccag gagagagaac caaaatgagt
     1741 gccattgaag ttaagaacct ggtgaaaaaa tttcacggtc agacggtgct gcacggtatc
     1801 gacctcgagg taaagcctgg cgaagtggtg gcaattatcg gtccgagtgg ttccggcaaa
     1861 accacgttgc tacgcagcat aaatctgctg gaacaaccgg aagcgggaac gatcaccgtt
     1921 ggcgatatca ctattgatac tgcacgttca ttaagtcagc aaaaatcgct gatccgccag
     1981 ttgcgtcagc acgtcgggtt tgtcttccag aactttaatt tgtttccgca tcgtacggtg
     2041 ctggaaaaca ttattgaagg gccggtgatc gtcaaaggcg aaccgaaaga agaggccacg
     2101 gcgcgcgctc gcgagctgct ggcgaaagtt gggctggccg gtaaagaaac cagctatcca
     2161 cgtcgtttgt ctggcggtca acagcagcgt gtagcgattg cgcgtgcgct ggcaatgcgt
     2221 cctgaggtga ttttgtttga cgagccaacg tcagcgctgg atccagagct ggtgggcgaa
     2281 gtcctgaaca caatccgcca gctggcgcag gaaaagcgca cgatggtgat tgtgacgcac
     2341 gaaatgagct ttgcccggga tgttgcggac cgggcgattt ttatggacca ggggcggata
     2401 gtcgagcagg gggcagcaaa agcgttattt gctgatcccc agcagcctcg cacccgccag
     2461 ttcctcgaga agtttctgct gcaataataa gaaaaaatca gccccgacga ttcacctgtc
     2521 ggggcgggac gccatttcaa gcgtgataaa actgcttaac aaatcagcat aactcattaa
     2581 taacataaga gaatgcgatg gcttgcaaaa gtaattcatt gcctgaataa tataaattat
     2641 atataaatct tatttatgtg atagtttgaa ttatcattat aaatgatact cactctcagg
     2701 ggcgttgcgg tttactatgc aggatacgga ttttttcagc tggcgtcgca cgatgctgtt
     2761 gcgttttcag aggatggagg ccgcagaaga ggtctaccat gaaatagagc ttcaggctca
     2821 gcagctggag tacgattact attcgttatg tgttcgccac ccggtaccat tcacgcgacc
     2881 taaagtggct ttctacacca attaccctga gtcgtgggtt agttattatc aggcaaaaaa
     2941 ttttctcgca attgatcctg tgctgaaccc tgaaaacttt agtcagggcc atttaatgtg
     3001 gaatgatgac ttattcagcg aagcacagcc attatgggaa gccgcgcacg cacatggttt
     3061 acgccgcggt gtcactcagt atttaatgct gccaaatcgg gcgctgggct ttttgtcctt
     3121 ttcccgttgc agcacgcgcg aaatacccat tcttagtgat gaactgcaat taaaaatgca
     3181 gttactggtg cgcgaaagtc tgatggctct gatgcgttta aatgatgaaa tagtgatgac
     3241 gccagagatg aatttcagca agcgcgaaaa agaaattctg aaatggacgg cggaagggaa
     3301 gacatcagca gagatagcga tgattttgtc aatctctgag aatacggtca atttccatca
     3361 gaaaaacatg cagaaaaaaa tcaatgcacc aaataagacc caggtggcct gttacgcggc
     3421 cgctactggc ttaatttgat cgcttttctt tctgcgtgcc agatgcaaaa atcggctgaa
     3481 aggcacgctc tcagccggtt ttatattact gacggtaggc ttgtttaatt tgcttaaccg
     3541 ttttggaaaa tactgcagcc tgggtttcgt cttcgatcag cgcaagctgt ttttccatct
     3601 ttaacatcac gcggcccgcg tcagcttgtc ccatcgcctg tagcatcagc gtcagcatcg
     3661 ctttcaggca ggagacttca tttgccagtt cttgattatt ctcggcagta gaaaaatcag
     3721 gcgtactcat ttattttcct cgtcatgttg caatgaaaat ttgcggtgaa aaatgttaag
     3781 gcggcggagt ataccataag ctttgctaaa aatagcagtg gttgtttttt gagcgtgacg
     3841 tcaacaatgc tattaaatgc gttaatataa tgaccaataa atatttttat catgaatgtt
     3901 ttttgcccgt attgttcggg ttaattaatg ttacatattc agcgggctga ttttcatttt
     3961 tgctgaataa agtcaatttt tgtcacattt catcgccggg cttactgtga aacgatcccg
     4021 gaagccgttg gtgacgggcg tgaccataac tgtggacaat cgaatttaca aaaacgagag
     4081 aaaaatcgaa tacccaccat ttttaacgtt tcaaagttgc aataaaaacc gctaatatac
     4141 gaatgactaa ctatcagtag cgttatccct atttctggag atattccttt gatcaacgtt
     4201 ctacttgttg atgaccacga actggtgcgc gcagggatac gacgcattct ggaagatata
     4261 aagggtataa aagtcgtcgg tgaggcatcg tgcggtgaag acgccgttaa gtggtgccgg
     4321 acaaatgccg ttgacgtggt gctaatggac atgagtatgc cgggcattgg cggtcttgag
     4381 gcgacgcgta aaatcgcgcg ttccacagct gatgtcaaaa tcatcatgct taccgtccat
     4441 acagaaaacc ctttaccagc gaaagtcatg caggccggtg ctgcgggcta cctcagcaaa
     4501 ggcgcggctc cgcaggaagt cgtgagtgcg attcgttctg tctattcagg gcagcgttac
     4561 attgcttctg atatcgctca acaaatggcg ttaagccaga tcgaaccaga aaaaacagaa
     4621 agcccatttg ccagtttgtc tgaacgtgaa ttgcagatta tgctgatgat caccaagggc
     4681 cagaaggtca atgagatctc agaacagctc aatctcagtc cgaaaacggt gaacagctac
     4741 cgctatcgta tgttcagtaa actaaacatt catggcgatg ttgagctgac tcacctggca
     4801 attcgccatg gtctgtgtaa tgcggagaca ttatcaagtc agtgagtgat cagtttgacg
     4861 caaaagcgtt tttaaaaacc gtaaccagcc agccaggcgt ttatcgcatg tacgatgctg
     4921 gtggtacggt tatctatgtc ggcaaagcga aagacctgaa aaaacggctt tccagctatt
     4981 tccgcagcaa cctcgcttcg cgcaaaaccg aagcgctggt cgcccagatc cagcaaattg
     5041 atgtaacggt tacgcataca gaaaccgaag cgctgttgct ggaacacaac tacatcaaac
     5101 tctatcagcc gcgttacaac gttttgctac gcgatgataa atcttatcct tttatcttcc
     5161 tgagtggcga tacccacccg cgtctggcga tgcatcgtgg tgcgaagcat gccaaaggtg
     5221 aatatttcgg cccgttcccg aatggctatg ccgtacgtga aacactggcg ctactgcaaa
     5281 agattttccc cattcgccag tgcgaaaata gtgtttatcg caatcgctcg cgtccgtgtc
     5341 tgcaatatca gatagggcgc tgtctgggac cgtgcgttga aggactggtg agtgaagaag
     5401 aatacgctca gcaggttgag tatgtgcgcc tgtttttgtc tggcaaagat gatcaggtgc
     5461 ttacgcaact cattagtcgt atggaaactg ccagccagaa tctggagttt gaagaagctg
     5521 cacgtattcg cgaccaaatt caggcggtgc gacgcgtcac cgaaaaacaa ttcgtttcca
     5581 ataccggcga cgacctcgac gttattggtg tggcgttcga tgcgggcatg gcttgtgtcc
     5641 acgtattgtt cattcgtcag ggcaaagtgc tcggcagccg cagctatttc ccgaaagtgc
     5701 ctggcggtac ggaactgagc gaggtggtag aaaccttcgt aggccagttc tatttacaag
     5761 gcagccagat gcgcacctta ccgggtgaga tcctgctcga ttttaatctt agcgataaaa
     5821 cgctgctcgc cgattccctt tcagaactgg cgggacgcaa gattaatgtt caaaccaaac
     5881 ctcgcggcga tagggcgcgt tatctgaaac tcgcgcgcac caatgcggcg acggccttaa
     5941 ccagcaaact ttcgcagcaa tctaccgttc accagcgact gaccgcgctt gccagcgtgt
     6001 tgaaattgcc ggaagtgaag cggatggagt gctttgacat cagccatacc atgggcgaac
     6061 aaaccgtcgc ttcctgtgtg gtgtttgatg ctaacggccc gctgcgtgcg gagtatcggc
     6121 gctataacat tacaggcatc acgccgggcg atgattatgc ggcgatgaat caggtgctgc
     6181 gtcggcgtta tggtaaagcc attgacgaca gtaagatccc ggatgtgatc cttatcgacg
     6241 gcggcaaagg ccagcttgcg caggcgaaaa atgtcttcgc cgaactggat gtctcatggg
     6301 ataaaaatca tccgctgcta cttggcgttg ccaaaggagc agatcgtaag gctgggctgg
     6361 aaacgctgtt ctttgagccg gaaggtgagg gattcagttt gccgccagat tctcccgcgc
     6421 tgcatgttat ccagcatatt cgcgatgaat cacatgatca cgcgattggc gggcaccgta
     6481 aaaaacgggc gaaggtcaaa aataccagtt ccctggaaac cattgaaggc gtcgggccaa
     6541 aacgtcggca aatgttgttg aaatatatgg gcggtttgca aggtttacgt aacgccagcg
     6601 tcgaggaaat tgcaaaagtg ccgggtattt cgcaaggtct ggcagaaaag atcttctggt
     6661 cgttgaaaca ttgatgtctc tgtagcaaca taggggtaat cttactgaca acagatagtt
     6721 acccgtcatt atgcaattta atatccctac gttgcttaca ctgttccgtg tcatccttat
     6781 cccattcttt gtattggtct tttatctgcc tgtcacctgg tcgccgtttg ccgccgcgct
     6841 cattttctgc gtcgcggcgg tgactgactg gttcgatggt tttctggcac gccgctggaa
     6901 ccagagtacc cggtttggtg ctttccttga ccctgtagca gataaagttc tcgtggctat
     6961 cgccatggtg ctggtaaccg agcattacca cagctggtgg gtgaccttac cggcagcaac
     7021 gatgatcgcc cgtgaaatta ttatttctgc gctacgcgaa tggatggcgg agttgggtaa
     7081 acgcagtagc gtggctgtct cctggattgg gaaagtgaaa accactgccc agatggtggc
     7141 gttggcatgg ctgctgtggc gtccgaacat ttgggttgag tacgccggta ttgcactttt
     7201 ctttgtggct gcggtactga ctctgtggtc aatgttgcaa tatttgagcg ctgcgcgtgc
     7261 agatttgctt gatcagtgat cgtttcggcg taattttcag caaacgatca aaagtggtga
     7321 aaaatatcgt tgactcatcg cgccaggtaa gtagaatgca acgcatcgaa cggcggcact
     7381 gattgccaga cgataataaa atcaagtgat taactgattg cttgatgaat gcgggaatag
     7441 ctcagttggt agagcacgac cttgccaagg tcggggtcgc gagttcgagt ctcgtttccc
     7501 gctccagttt aaaagacatc ggcgtcaagc ggatgtctgg ctgaaaggcc tgaagaattt
     7561 ggcgcgttaa caaagcggtt atgtagcgga ttgcaaatcc gtctagtccg gttcgactcc
     7621 ggaacgcgcc tccactttct tcccgagccc ggatggtgga atcggtagac acaagggatt
     7681 taaaatccct cggcgttcgc gctgtgcggg ttcaagtccc gctccgggta ccatgggaaa
     7741 gataagaata aaatcaaagc aataagcagt gtcgtgaaac caccttcggg tggttttttg
     7801 tgcctgcaac ttgtcgttac accctcctta atttttaatc accggcaaag ccactcaaaa
     7861 agtatctctg atacggaccg gcatgtaaga taggtgctgg cgagttgaga tccacaagga
     7921 aaagcgtatg aaaacgggac cgttaaacga aagtgagttg gaatggctgg acgatattct
     7981 gaccaaatac aacactaacc acgccatcct tgatgtggcg gagctggacg gtttattgac
     8041 ggcggtgttg agttctccgc aagagattga accggaacag tggctggttg ccgtgtgggg
     8101 tggggctgac tatgtgccgc gctgggcgtc agagaaagag atgacgcgct ttatgaacct
     8161 ggcttttcaa catatggccg ataccgcaga gcgtctgaac gaattcccgg agcagtttga
     8221 gccgttattc ggcttgcgag aagttgatgg cagcgagctg acgattgttg aggagtggtg
     8281 ctttggctat atgcggggcg tggcactttc tgactggtca acgttgcccg attcgttaaa
     8341 accagcgctg gaggcgattg cgctgcacgg tactgaggaa aacttcgagc gggtagaaaa
     8401 gatgtcgcca gaagcgtttg aagagagcgt agatgccatt cgactggcgg cgcttgatct
     8461 acatgcgtac tggatggcgc atccgcagga aaaagctgtt cagcaaccga tcaaagcaga
     8521 agagaaaccg gggcgtaacg atccttgccc gtgcggtagt ggtaagaaat ttaagcagtg
     8581 ctgcctgcat taagaggcca aattgggtgt cagcgttaat tctggcaccc aataaggaat
     8641 ttgaggctat ctgatcaccc cacttctggt aacaaccctg ccgcaatcaa aaattgcacg
     8701 ccaatcacag caataccaca gagaaacacc accaccagcg ccggacgacc acctttgacc
     8761 cggtaacccg cctgaggatt gtgctttctg ctttgccagg tcaacagcga agggataatc
     8821 aatgccagta ccgccagcgc cacaccggcg taacccagcg ccatcacaaa tcctcgtgga
     8881 taaaacagtg caaacgccaa cggcggcaga aaggtaattg caccagtttg caaccgtcca
     8941 ccaacggtat ttgaacgctg aaataaatca gccagataat caaataagcc taacgcaacg
     9001 ccgagaaatg acgtggcgag ggctaaatca gcaaataaat gcactgccag ctcaacatgc
     9061 ggagaggcca ccatttcgcg taacgcctgt aacagcccgt ttaatccagc atgattagcc
     9121 agcaatccca taaaggttgt tgaatcaatg ctgccaagcg ttgccacctg ccagaaaata
     9181 tatgccacca gggggatcgc actaccggtt ataaacaccc agcgtagctt acgaacgttg
     9241 ccatccatat agctgacaat actcggcacg ctaccgtgaa aaccaaatga ggtaaaaatc
     9301 accgggattg ctgacagagc cagcccctgt tgcaacggca gggttaaaag attcactttg
     9361 tgaatatgcg gtagcagcaa taccagcatc accaccagaa aaataatttt ggcgctgaac
     9421 agaaaacggt taaataaatc gaccagcgat gttccaacac aaaccacgcc accggcaaca
     9481 aaagtgaaca acagcacgcc agcggttgcc gacattgaaa tacctgtcca gtcgctgatg
     9541 ctggaggcca acaattcacc gacaccgctg atgtatgccg cagtcagagc atacattaag
     9601 aacatcatac tgaagcccgt cagccattga ccgtagcgtc ccagatagcg ttttgccagc
     9661 gtgcccagac cggtatctgc cggaacatgc tggtacacct ccagcagtaa tagcgccgtg
     9721 tagcacatca acgcccaaag cccaatcaac aagattaacg taacgctaaa accaacaccg
     9781 gccgcagcca gcggcattgc cagcatgcct gcgccaattg tggttcccgc cacgataaaa
     9841 acacttccca gggttctgtt tttcacgctt tcttctgtcc tgacgatctt tatgagtgat
     9901 atctgcggcg caggttaagg cataacggct gcttcgtcaa accgacgtta catatagtgt
     9961 aaatataaat gtacaataaa aagcattcag tcctgcgctg gcgcaaagtc ttcataagcg
    10021 gcaatcgcta cgctaggctt tttttgagga ggaacgatgg actctattca cggtcatgaa
    10081 gtgttaaata tgatgattga atcaggcgag caatatacgc atgccagtct ggaagctgcg
    10141 attaaagcgc gttttggtga acaggcacgt tttcacacct gctcggcaga agggatgaca
    10201 gcgggagagc tggtagcgtt tctggcagca aaaggcaaat ttataccttc gaaagacggt
    10261 ttttcgaccg atcagagtaa gatttgccgt cactgatttg aaacggcggc agtaaacctg
    10321 ccgccggaga gcattagttt tgtgtgtcga gggtagagag ttctttgtcg ataaaataca
    10381 gaccttcgcc gcttttgcct gccaggctta atttatcaat aatcgatttg aacagtttct
    10441 cttcttcatg ctgctcagaa acataccact gcaggaaatt aaatgttggg tagtcctgat
    10501 tggtcattgc agcatgagcc agttcgttaa ttttctgggt gattaattgt tcgtgtttat
    10561 aggtttcctg gaataattca tcaagtgagg aatattcagc aaacggagat tcaacggtat
    10621 taatacgcgg taaattgccg gtatcagtca ggtaatcaaa cagacgctgc atatgcgtca
    10681 tctcttcctg ggcgtgacgg cgcaggaacg cggcagcacc ttcgaaggta tgatagctgc
    10741 accaggcgct catttgctga taaagcagtg aagagtacag ttccaggttc atctgctcat
    10801 taagtttttc aatcatttct ggtttcagca tgatagtgct ccacaaaggt tatatttgtt
    10861 gctgtggcgt cactataatt tgttatttta ttatttgcaa aaggtaaaat ataaacttca
    10921 attattaaaa atacgaat
//
LOCUS       DAZPBM010000138        10835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPBM010000138 DAZPBM010000000
VERSION     DAZPBM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.139"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..279)
                     /locus_tag="ACHUZQ_003240"
     CDS             complement(<1..279)
                     /locus_tag="ACHUZQ_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1026859.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(446..1186)
                     /locus_tag="ACHUZQ_003241"
     CDS             complement(446..1186)
                     /locus_tag="ACHUZQ_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1026860.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHUZQ_003242"
     CDS             complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHUZQ_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1026861.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHUZQ_003243"
     CDS             complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHUZQ_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1026862.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHUZQ_003244"
     CDS             complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHUZQ_003244"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1026863.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHUZQ_003245"
     CDS             complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHUZQ_003245"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1026864.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHUZQ_003246"
     CDS             complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHUZQ_003246"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1026865.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7002..7433
                     /locus_tag="ACHUZQ_003247"
     CDS             7002..7433
                     /locus_tag="ACHUZQ_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR1026866.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7456..8034
                     /locus_tag="ACHUZQ_003248"
     CDS             7456..8034
                     /locus_tag="ACHUZQ_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1026867.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8035..8742
                     /locus_tag="ACHUZQ_003249"
     CDS             8035..8742
                     /locus_tag="ACHUZQ_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR1026868.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8730..9407
                     /locus_tag="ACHUZQ_003250"
     CDS             8730..9407
                     /locus_tag="ACHUZQ_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1026869.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9401..10078
                     /locus_tag="ACHUZQ_003251"
     CDS             9401..10078
                     /locus_tag="ACHUZQ_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1026870.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10050..10763)
                     /locus_tag="ACHUZQ_003252"
     CDS             complement(10050..10763)
                     /locus_tag="ACHUZQ_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1026871.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10760..>10835)
                     /locus_tag="ACHUZQ_003253"
     CDS             complement(10760..>10835)
                     /locus_tag="ACHUZQ_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1026872.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2880 c         2699 g         2676 t
ORIGIN      
        1 tcgtgaacga cgccctgagt tatggatttc attatcaatc gcagaaagtc gttgataaac
       61 cgggaacagt ttttgataca gttcctgacc ggctttattg aggctgatat tattatcttt
      121 gcgggtaaat agcgtatagc caatttgttc ttccagcgcg gcaatgcttt taccaaatgg
      181 cgaagccgtc atatggattt tttctgccgc ccgggcaata ttattggttt gcgctaataa
      241 aatgaaattg cgcatctttt tcgaaataaa aatgtccatc ccccctcccc gcatccttgt
      301 ccgcttaacc gtttcagtca gcctatcctt gatgaaaccg cgagcaaaga taggtgatta
      361 cgtcatggtt ttacagaaaa ttacagaaaa aggaggcaat atcgggtaaa ggcattagcc
      421 cgacgaatac gtcgggctac aagtattatt gtgctgcagg tgttttagcg ggttgttgat
      481 ccacaggttc taactggaag accacatcga cctgatcatc aaactgaata gcggcctgct
      541 cgtaagtttc ctgggcagac accggcgcgg catcggcttt catcatccgc accattgggc
      601 tgggctgata gttggaaaca tggtagcgca cgctatatac cggccccagt ttacgatgaa
      661 agccgttcgc cagttcctgc gcctgatgaa tcgcgttatc aatcgctgcc ttacgcgctt
      721 tgtctttata ggcatccggc tgcgccacgc ccagcgacac agaacgaatt tcgttaagac
      781 ccgccttcag cgcgccatcc agcaaggaat tcagtttgtc taactgacgg agcgtgactt
      841 ccaccgttct cacagcgcgg tagcctttca ggatactttt accatcctga taatcataat
      901 ctggctgggt gcgtaagttc gctgagctga tatctttttt cgcgatctga ttgagttcaa
      961 ggaaggaaat gtattgtgcg acgcgctcat ctgcctgttt cttggctgta gcggcatcct
     1021 tcgcggccac gttaacttca atcgcaagag tggcaatgtc tggcaccgca tccacacttg
     1081 ccgtaccgga ggtgacaata tgcggtccat ccggcaattc gttagcctgc gctgccatcc
     1141 cgctaatacc cattaatgcc gccagggcga taactttgaa cttcacgtta gtctctccgt
     1201 gttaattgac ttgcccaaaa ttcaggacct ctggaggcaa gcttagcgcc tctgttttat
     1261 ttttccatca gatagcgcct aactgaacaa ggcttgtgca tgagcaatac cgtctctcgc
     1321 cagctgcaag gctataaacc acatgacaca tcccactacc agattgataa tgcgctgtga
     1381 ttttgccgtg cgcaggcgcg gtgccagcca ggctgcgaga atagccagac caaagaacca
     1441 caggaaagag gcgctaattg tcccgagcgc aaaccaacgt tttggttcga cgtcaagctg
     1501 cccgccaagg ctgcccagta caacaaaagt atccaggtaa acatgcggat tcagccaggt
     1561 cactgccagc atggtggcga taattttcca tctgccttgc ttcaggactt cggcactagc
     1621 taactcaata ttactactca tcgctgtttt aaaagcgcca aaaccatacc acagcaagaa
     1681 ggctacgccg ccccaggtga ccagcgccag caaccacggc gactgcatca ataacgcgct
     1741 gccaccaaaa atcccggcgc aaatcaggac caaatcgctg atagcacaaa gtaaggcaat
     1801 cataatgtgg tactgacgac gaatgccctg attcatcaca aaagcatttt gtggaccgag
     1861 cggcagaatc atagctgccc caagagcaat accttgaaag taataagaaa acacgttgga
     1921 tattccgaat taagtatctg atgcagagac tatatcgcgg gattgttatt agcggaaatt
     1981 gataatatta attggcaatc agaataacta ataagagaga ggccagagag tattatctgg
     2041 cctcagcgtt gattacgcag ctttgtcttc tttcacccgc ttaaagttca catccatttg
     2101 cgggtacggg aagctgatac cggcggcatc aaattcacgt ttaatacgct ccagcacatc
     2161 ccagtacacg ttttgcagat cgccgctgtt gctccagacg cggaccacga aattaatcga
     2221 cgatgcacca agttcgttca gacgcacagt catttcgcga tctttcaaaa tgcgatcttc
     2281 agactggata atattggtca ggatctgctt aacctgatcg atatcggaat catacgccac
     2341 gccaataata aattcgttac ggcgaactgg ctcgcgagag aagttaataa tatttccggc
     2401 aataatttta ccgttcggaa taacgataat tttaccgtct gcagtacgca tggtggtgga
     2461 gaaaatctgc acactcagca cagtaccggc tacgccgccg agatcaacat attctccggc
     2521 acggaacggg cggaacatga caagtaacac gccagcggcc aggttagaaa gtgacccctg
     2581 caaagccaga ccaacagcta agcctgcggc accgagtaca gcaatgactg acgctgtttg
     2641 tacacccacg cgtcccaggg cagcgattag cgtaaaggcg ataataccgt aacggactaa
     2701 tgcagaaaga aaatcagcaa cagtggcatc gattttacgg gagatcatca ggcgattcac
     2761 cgcgttggaa atcatccgcg cgataatcaa accaacgatg atgatcgcga gtgccgccac
     2821 gatgtttact gcataactta gcagcagcgc ctggttagct accagccagc ttcccgcgcc
     2881 gtttatgcta tcgacaacat tcaaatcttc cattcaatat tccttttcaa ctgactcaaa
     2941 atggagaaat acactccgcc gccttatgac gggcagtctg acaagggtaa acaataaagc
     3001 ataattttgc caaatagatc acagatttct catctgaatc accttgcttt tggcgaaaaa
     3061 aagacccgca gagcgggcct tgagataagc agaaaggaat atcttacaga acgtcgatcg
     3121 cgttcagttc ctggaatgct ttttccagac gagcgatcat cgaagtctga ccggcacgca
     3181 gccatacgcg cggatcgtag tatttcttgt tcggctgatc ttcgcctttc gggttaccca
     3241 actgcccctg cagataagct tcgttcgctt tgtagtagtt cagaacgcct tcccaggttg
     3301 cccattgggt atcggtatcg atgttcattt ttactacgcc gtagcttacg gagtctttga
     3361 tttcctgagc agtagaaccg gaaccaccgt ggaatacgaa gttcaggctg ttgtgcggca
     3421 ggttgtgttt cttggaaaca tattcctgag aatcacgcag gatggtcgga gtcagaacca
     3481 cgttacccgg cttgtaaaca ccgtgtacgt taccgaagga cgcagcgatg gtgaaacgcg
     3541 ggctgatttt gctcagttcg gtgtatgcgt aatcaacgtc ttccggctgg gtgtacagtg
     3601 cagaagcgtc catgtggctg ttgtccacgc cgtcttcttc accaccggtg caacccagtt
     3661 cgatttccag agtcatgccg attttggaca tgcgctccag gtatttagag cagatttcga
     3721 tgttctcttg cagagattct tcagacaggt cgatcatgtg agaagagaac agcggcttac
     3781 cggtagctgc gaagtgtttt tcacccgcgt ccagcagacc gtcgatccac ggcagcagtt
     3841 tcttcgcgca gtggtcagtg tgcaggataa ccggaacacc ataatgttca gccatctggt
     3901 gaacgtgatg cgcaccagag atcgcgccca ggatagcagc accctgcgga acgtcagatt
     3961 tcacgccttt accagcgata aaggaagcac caccgttgga gaactgaacg ataaccggcg
     4021 ctttaacttt agcagcggtt tccagtacgg cgttgatgga gtcagtaccg acgcagttta
     4081 ctgctggcag tgcgaagttg ttttcttttg ctacctggaa aactttctgt acgtcatcac
     4141 cagtgattac gccaggtttt acgaaatcaa aaatcttaga catgtctctt gtcctgtatc
     4201 gtcgggcctt atactcatca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc
     4261 cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg
     4321 aattatttag agtatgaaaa aatgcgtgct ctaaaagcgc gctgaaacaa gggcaggttt
     4381 ccctgccctg tgatttttta cttcttagcg cgctcttcga gcatcgctac tgcaggcagt
     4441 actttacctt ccacgaattc gaggaatgcg ccgccgccag tggagatgta ggagattttg
     4501 tcagcaatgc cgaacaggtc gattgctgcc agagtgtcgc cgccgccagc gatggagaac
     4561 gcttcgctgt ctgcgatagc gttagccacg atttcagtac ctttgcggaa gttcgggaat
     4621 tcgaacacgc caaccggacc gttccacaga atggttttcg cattcttcag gatttcagcc
     4681 agttcctgag cggaagcatc accgatatcc aggatctgct cgtcagcttt cacatcgtta
     4741 acagatttca gggtagccga tgcagtttca gagaactcgg ttgctacgcg aacatcagac
     4801 ggaaccggga tgttgcaggt ggtcagcaga cgtttagctt cgtcaaccag gtcagcttcg
     4861 tacagggatt tacccacatc gtggccttgt gccgcgataa aggtgttagc gataccacca
     4921 ccaacaatca gctggtcagc gattttagac agggagtcca gaacggtcag tttggtagat
     4981 actttagaac caccaacgat agccaccatc gggcgagcag gttctttcag tgctttaccc
     5041 agcgcgtcca gttcagctgc caacagcggg cctgcgcacg caacatcagc gaatttaccg
     5101 ataccgtgag tagaagcctg cgcgcggtga gcagtaccga atgcgtccat tacgaacacg
     5161 tcacacagtg cagcgtattt tttggacagg gtttcgtcgt ctttcttctc gcctttgttg
     5221 aagcgaacgt tttccagaac aaccagttca ccttcagcaa cgtcaacgcc gtcgaggtaa
     5281 tctttaacca gacgaaccgg gttagacagt ttgtctttca ggtagttaac aaccggcagc
     5341 agagagaatt cttcgttgta ctcgccttcg gtaggacgac ccaggtggga agttaccatc
     5401 actttcgcgc cttgtttcag ggccagttcg atggtcggca gagaagcacg gatacgcgcg
     5461 tcgctggtta ctttcccgtc ttttactggt acgttcagat ccgcacggat aaatacacgt
     5521 ttcccagcaa gatccagatc ggtcatctta attacagaca tggtgaatcc tctcgttgat
     5581 tctaaaagtt ttgcagacgc tgcttgcgtc ttacctgaaa gcaacagtag ccatagctaa
     5641 cgtcgtgtcg agcattcggt tagcaaagcc ccattcgtta tcgcaccaga ccaacgtttt
     5701 gatcaggtgt gcgccactga cccgggtttg ggtgccatcg acaatggcac tgtgcggatc
     5761 gtggttaaaa tctacagaga ccaacggcaa ttccgtatag tcaactatac catgaaatgc
     5821 accttgtgct gctttttgca gcaacaggtt gacttcattg gcttttacag gtttcttcac
     5881 cgtcacgctt aaatcgattg ccgtcacatt tatggttggc acacgtaccg caatcgcttc
     5941 aaagcgatcg ttaaattgcg gaaaaaatcg tgtgataccg gcggccagtt tagtatcgac
     6001 cggaatgatc gactggctgg ctgcccgggt gcgacgcagg tcaggatggt atgcatcaat
     6061 aacctgttga tcgtgcatgg cggagtgaat tgtggtcaca gtgccggact caataccgta
     6121 cgcatcatct aacaatttga tgacgggaat tatgcaattc gtggtacagg aagcgttaga
     6181 aacgatgcgg tgttccgcac gaagttgatc ctgattgacg ccgtaaacaa cggtcgcgtc
     6241 gagatcgtta ctgccaggat gtgaaaagag cacttttttg gccccggcgg caatatgcgc
     6301 ttcgccatgc tcgcgggagc catatacgcc ggtgcagtcg aggactacat caacgccaag
     6361 ttcacgccag gggagcgatt gcagtgaacg ttcatgcaat acgcggatgg cgtcatcacc
     6421 aacaaaaagt tgatcgcgtt cctgtcgtac ttcccatgca aaacggccat ggctggtgtc
     6481 atatttcaac aaatgcgcca tgcccgcagc atccgccagt tcgttgattg ccaccacggt
     6541 aatttccgcc cggcgtccgg attcatacaa agcacgaacc acattacgcc cgatgcgacc
     6601 gaagccattt atcgctacgc gtacggtcat agatctcctg caaggttttc cctgagcaaa
     6661 tttgccagac agagtaatcc agcaaatcgt ccggggaaac cttacctgtc gcaaactgcg
     6721 actgattggt taattgtcga acatttaatc gactgaaacg cttcagctag gataagcgaa
     6781 acgtggaata aaaggaatga ttgtccagtc aaagaaaaca attatctgac ttacgtcacg
     6841 ttttcagccc cgcccctgtg gaatttattc cactttgcag aatcacgaat aagcgtagca
     6901 aaatcttttc gttgtgccgt cacaaaccct gacttatatc agaatatgac tgatgcagcg
     6961 tcgatattat ccgcaatacg tccgagtttc ggagtactcc aatgatgaca ttgccctacc
     7021 catattcttc ccccgcacgc ttttatgcac tgcgcctcct ccccgggcag gaagtttttt
     7081 cccagctaca cgcttttgca cagcagcatc aacttcatgc cgcctggatt gcaggctgta
     7141 ccggtagcct gactgacgtc gccttgcgct atgccgggca agaaggcacc acgctcctta
     7201 acggtacatt cgaagtgatc tcactaaacg gcacgctgga acagagtggt gagcatcttc
     7261 atctctgcgt ttccgatcca cacggcacga tgcttggcgg tcatatgatg cccggctgta
     7321 ccgtgcggac cacgcttgaa ctggtgatcg gtagccttga ggaactggcg tttagccgcc
     7381 aaccttgcgc gctttctggt tacgacgagt tgcatatttc ccccgttaaa taactacttt
     7441 caaagaggtt ttcttatggc gcgtagccac ttttccagtc aggcactggt gttgatcgtt
     7501 atatctatcg ccatcaatat gatcggcggg caactggcca gcatggtcaa attacctata
     7561 tttctcgact ccatcggcac acttattagc gcggtactgc ttggtccggt gatcggaatg
     7621 ctgaccggct tactgaccaa tttactctgg ggattgctga ccgacccgat cgccgccgca
     7681 tttgcacccg tcgcgatggt catcggcctg gtcgccggtt ggctggcgcg agcgggttgg
     7741 tttcgcaccc tgccaaaagt cgttgtcagc ggcgtgatta ttacgcttgc ggtaacggtc
     7801 gtcgctgtac cgctgcgtac tgcgctgttc ggcggtgtca ccggtagcgg agccgatttg
     7861 ttcgttgcct ggatgcactc gatggggcaa aacctggtgg agtcggtggc gattacggtg
     7921 ataggcgcga acctggtcga taaaattctt acagcggtga ttgtctggct tttactgcgc
     7981 cagctgccga ttcgcaccac gcgccatttt ccggcaatgg ctgccgtgcg ctaaatgcat
     8041 ccatttacct cattaacact gtgggcattg gcggcctgca ccacgcttat cctgcccgcg
     8101 cagaccattt tgccgatcta tagcgcggcg accttttttt gtcttattgc gctaaaggcc
     8161 acgcggcggc gggcaaaata tgtcgcctgg ctgatgttct cgctgggagc cgggctgtgg
     8221 ctggtacacg gtggctggct aacagaatgg ctaagcggca cgccgcgttc gccggagcgt
     8281 tggacgcatg ctattacgct ctggttgcgg attctggcga ttgtttcaac ctcacagttg
     8341 tggatgcagt acgtccccgt gcaatgtttt atccgcgctc tgtttgcttc tcgcctgccg
     8401 ccaggcgtcg cttatctgtt tgccggaccg ttattggtcg ttgagcagtt aaaacggcaa
     8461 ttagcgatta ttcatgaagc gcagcgtgcc cgcggcgtgc cgctggatga gggctggtat
     8521 cagcgtttac gtgcgatgcc cgcgttaatt attctgctaa cacacaatgc gcttaacgat
     8581 ctggctgtcc gtggtgcggc gctggatatg cgggcatttc ggatccacaa ccgacgaaca
     8641 acgttgtggg caccagccga tagcacccta caacgcgtag cgcgttacac catgatcttg
     8701 ctgatgctaa cagaattcgg agcgtggata tggttacgtt agaacagttc cgttattgcc
     8761 cgacacactc aacgcatcct ccgttctgtt atgactttca ctatgtcaaa ccggggatgg
     8821 tcgccatctt tggtgacaac ggcagtggca aaagtacgct ggcacagctg atggcgggct
     8881 ggtatccgga ctttctgcca ggagagataa caggcacggg gacgctcctc ggtacgccca
     8941 ttgggcatct cccgcttaat gaacaatccg ccaccatcca gctcgtccag caatcgccct
     9001 atttgcagct ctccggctgt acctttagcg tggaagaaga agttgccttt gggccagaaa
     9061 atctgtgtct tgctgaaaca gagattatgg cgcggattga tgccgccctg actctgaccg
     9121 agtgccagcc gttgcgccat cgtcacccgg caacactctc cggtggagag acacaacgcg
     9181 tggtcatcgc ctgcgccatc gcgatgcagc caaagctctt aattcttgat gaagccttta
     9241 gtcgcctgac gccgcaggcc agcgaaatgc tgttgcagcg cttacaacac tgggcgcttg
     9301 aacgtggttc gctgattatt ctttttgaac gtcatcgcac acctttcctg aactactgcc
     9361 agcaagcgtg gcaattgcaa aacggagcgc tccagccact atgttaacgt taaatcagat
     9421 atcttatcgc tggcctagcg cagcaacgga ttgtctttgc gacatttctc tgcaactcaa
     9481 acaaggtgaa tggttggcgc tgaccggcga caatggcgcg ggaaaatcga cgctgttacg
     9541 agtaatggca ggtcttctta cgcccacagc cggtacggtt atgctgcaac aacaggcgat
     9601 gaaaaacctg aaaaaccgcc agcgcgcggc aaaggttggc gtactgtttc aggaagcgga
     9661 aaaccaactt tttcacagca ccgtcgctga cgagatcgca tttggtttga aactgcaaaa
     9721 atgcccggcg gatgaaatca ctcaacgtac ccatgctgcc ctgcaatgtt gtcagttagc
     9781 agacactgcc aactcgcacc cgttggatct gcatagtgcg cagcgacgga tggtcgccgt
     9841 cgcctgcctg gaagcactct ctccgccact attactgctg gacgagccga gtcgggattt
     9901 tgacgaaaat tggttgagcg tgtttgaaag ctggctggag aagtgccgcc agcgaggcac
     9961 gagcgtggta gcaatcagtc atgacgccgc atttacccgg cgtcatttct ctcgtgtggt
    10021 gcgactggaa gatggtgtaa tcaggaacgt taatccacca gacgatattc acccgtagcg
    10081 gtcatcatta aggtcaaatt tgcaggcaga ctctcttcca gcacccggcg aacattcggc
    10141 ccgtcggtac ggtcataaaa ggcttctgca tctgccagcg atacccctcc cgccagcttg
    10201 cgccccacca gacgctcccg caacgcagta gctggcgcgt tgataaagat cgagaaatca
    10261 caaaattgcg caagctggca ccacttctcg tcgtccagca acaaccagtt tccttcgacg
    10321 atgacgagtg gtgcggtaac gtgcaacgca tcttcaacag gatcatgctt ttgtcgatcg
    10381 tactgcggcc acgtacaatc cccttccacc acctggcgca gattttccgc cagtttcgca
    10441 acgtcaaatg tctctggtgc gcctttgaag gggcgcaatt gatgcgcgtc cagccagcta
    10501 ttgtaatggt gaaaaccatc catcgggagc gtctgaatag cgggcagctc cgggtcttgt
    10561 tgcacgagat attcccagaa ggtggttagg gtagatttac ccgtccccgg cggagcgcaa
    10621 agaaaaacca ccgtacgccg ctgcggattt accgtctgta aggccgccag catacgcagc
    10681 aacggtttat gcacgcgttc tatctcttca tcatggtact gcgcttgcac gttcagccca
    10741 ttcaccgtta attcaatttt cacggcgtta attcctttaa ggtggtcgtt actgccagcg
    10801 tcagcgccgt tttgaccatt ccgctaaaac gcgcc
//
LOCUS       DAZPBM010000139        10812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPBM010000139 DAZPBM010000000
VERSION     DAZPBM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.140"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHUZQ_003254"
     CDS             complement(<1..696)
                     /locus_tag="ACHUZQ_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1026873.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHUZQ_003255"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHUZQ_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1026874.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUZQ_003256"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUZQ_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1026875.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUZQ_003257"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUZQ_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1026876.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUZQ_003258"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUZQ_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1026877.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUZQ_003259"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUZQ_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1026878.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUZQ_003260"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUZQ_003260"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1026879.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUZQ_003261"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUZQ_003261"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1026880.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUZQ_003262"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUZQ_003262"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1026881.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHUZQ_003263"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHUZQ_003263"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1026882.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHUZQ_003264"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHUZQ_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1026883.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUZQ_003265"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUZQ_003265"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1026884.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUZQ_003266"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUZQ_003266"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1026885.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUZQ_003267"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUZQ_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1026886.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         2715 a         2661 c         2878 g         2558 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 tctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcaagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa ac
//
LOCUS       DAZPBM010000140        10739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPBM010000140 DAZPBM010000000
VERSION     DAZPBM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.141"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..553
                     /gene="pldA"
                     /locus_tag="ACHUZQ_003268"
     CDS             <1..553
                     /gene="pldA"
                     /locus_tag="ACHUZQ_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1026887.1"
                     /translation="VLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAG
                     WTLRDVEMGYNHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDI
                     TKYMGYYQLKIGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYG
                     ESLIDYNFNQTRVGVGVMLNDLF"
     gene            686..2515
                     /gene="recQ"
                     /locus_tag="ACHUZQ_003269"
     CDS             686..2515
                     /gene="recQ"
                     /locus_tag="ACHUZQ_003269"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1026888.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            2579..3196
                     /gene="rhtC"
                     /locus_tag="ACHUZQ_003270"
     CDS             2579..3196
                     /gene="rhtC"
                     /locus_tag="ACHUZQ_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1026889.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(3258..3878)
                     /gene="rhtB"
                     /locus_tag="ACHUZQ_003271"
     CDS             complement(3258..3878)
                     /gene="rhtB"
                     /locus_tag="ACHUZQ_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1026890.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            3989..5011
                     /gene="pldB"
                     /locus_tag="ACHUZQ_003272"
     CDS             3989..5011
                     /gene="pldB"
                     /locus_tag="ACHUZQ_003272"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1026891.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            5019..5819
                     /gene="yigL"
                     /locus_tag="ACHUZQ_003273"
     CDS             5019..5819
                     /gene="yigL"
                     /locus_tag="ACHUZQ_003273"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1026892.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            5895..6794
                     /gene="bioP"
                     /locus_tag="ACHUZQ_003274"
     CDS             5895..6794
                     /gene="bioP"
                     /locus_tag="ACHUZQ_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1026893.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(6682..7635)
                     /gene="metR"
                     /locus_tag="ACHUZQ_003275"
     CDS             complement(6682..7635)
                     /gene="metR"
                     /locus_tag="ACHUZQ_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1026894.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            7871..10132
                     /gene="metE"
                     /locus_tag="ACHUZQ_003276"
     CDS             7871..10132
                     /gene="metE"
                     /locus_tag="ACHUZQ_003276"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1026895.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(10254..10376)
                     /locus_tag="ACHUZQ_003277"
     CDS             complement(10254..10376)
                     /locus_tag="ACHUZQ_003277"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026896.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(10417..10662)
                     /locus_tag="ACHUZQ_003278"
     CDS             complement(10417..10662)
                     /locus_tag="ACHUZQ_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR1026897.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         2448 a         2716 c         2943 g         2632 t
ORIGIN      
        1 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
       61 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
      121 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
      181 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
      241 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
      301 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
      361 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
      421 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
      481 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
      541 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
      601 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
      661 tttatttcag gcaatcgggg tgaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
      721 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
      781 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
      841 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
      901 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
      961 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     1021 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     1081 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     1141 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     1201 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     1261 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     1321 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     1381 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     1441 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     1501 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     1561 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     1621 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     1681 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     1741 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     1801 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     1861 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     1921 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     1981 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     2041 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     2101 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     2161 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     2221 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     2281 tcgcaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     2341 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     2401 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     2461 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     2521 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     2581 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     2641 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     2701 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     2761 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     2821 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     2881 tgcgccacag gtcgagctgg cgaaaagtgg gcgcagtttc ctgaaaggtt tactgaccaa
     2941 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     3001 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     3061 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     3121 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     3181 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     3241 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     3301 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     3361 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     3421 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     3481 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     3541 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     3601 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     3661 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     3721 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     3781 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     3841 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     3901 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     3961 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     4021 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     4081 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     4141 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     4201 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     4261 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     4321 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     4381 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     4441 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     4501 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     4561 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     4621 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     4681 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     4741 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     4801 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     4861 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     4921 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     4981 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     5041 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     5101 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     5161 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     5221 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     5281 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     5341 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     5401 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     5461 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     5521 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     5581 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     5641 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     5701 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     5761 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     5821 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     5881 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     5941 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     6001 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     6061 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     6121 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     6181 tctacatcac gctgatttat gacatcatga gtaagcgccg tctgcgctgg ggctatgctt
     6241 ttagcgcctt gctggcggtg attggtgccg ggattattcg ctatgatcag gttaccgacc
     6301 atttctggac tggcttgttg ctggtgcaac tctccaatat cacttttgcc atcggcatgg
     6361 tgggttacaa acgcctgatg gaaactcgcc cgatgccgca gcataacgcc tttgcgtggt
     6421 tctatcttgg cgcgttcctg gtggcggtaa ttgcatggtt cttgctggga aatgcgcaga
     6481 aaatgccgca aaccacgttg caatggggca ttctggtgtt tcttggcgtg gtggcttccg
     6541 ggattggcta ctttatgtgg aactacggcg cgacgcaggt ggacgccgga acgctgggca
     6601 ttatgaataa tatgcacgtt ccggcagggc tgctggtaaa cctggctatc tggcaccaac
     6661 agccgcactg gccaacgttc attacaggcg cgctggtgat cctggcctca ctgtgggtgc
     6721 atcgtaagtg ggtcgctccg cgctcttcac aaacggcaga tgatcgcagg cgtgattgcg
     6781 cgctgagcga ataaatgctt ctgtaatcgg ctgacgctgc tcgccatcgc gcacggcggc
     6841 gtacagtcgg ctccacaagc cttcgcccag ggttttggtc accaccagac cctggcgctc
     6901 aaaactctct actacccaat gcggtagcgc ggcaataccc atccgcgcgg caaccatctg
     6961 aatcaacaat aaggtgttat cgacgctttt cagtgacggg ctgacgcctg ccggctgaag
     7021 aaaatgccgc cagacatcca gtcgactacg ctgcaccgga taaatcaaca gcgtctcgct
     7081 ggcgagatct tccggtgtaa ttcgcgtttt cgccgccagt ggatggtcag gtgctaacac
     7141 cagacgcact tcatagtcga acatcggcga ataatgcagg ccactgcgcg gcaggatatc
     7201 ggaggtcatc accagatcca gctcgccctg ttgcaacgca ggctgcgggt caaacgtcac
     7261 gcccgattta aaatccatct ctacctgcgg ccagttctta tggaaatttt ctaacgcggg
     7321 cgtcagccac tgaatacagc tatggcactc aatggcaatg cgcagacgcg tctgctgcgg
     7381 ttcattgcag gcttgcagtg cctggctgat ttgcggcagc acctggtttg ccagttgcaa
     7441 caggatttct ccctgcggtg taaagcgtag cggctggctc ttacgcacaa atagccggaa
     7501 gccaaggcgt tgttccagat cgctaaactg gtgagacagg gcggattgcg tctgatgcaa
     7561 cgtcgccgca gcggctgcga gagagccgca gttccgcaac gcttgtagcg ttttcaggtg
     7621 ttttacttcg atcatgaaag tccttcactt cggcatgaat aatttgcgct tgaggaatat
     7681 acagtaaccg ccaattatgg atgtgtaaac atctggacgg ctaaaatcct tcgtctttta
     7741 aatttatggt gcgttggctg cgtttctcca ccccggtcac ttacttcagt aagctcccgg
     7801 ggatgaataa acttgccgcc ttccctaaat tcaaaatcca taggatttac atataattag
     7861 aggaagaaaa atgacaatat tgaatcacac cctcggtttc cctcgcgttg gcctgcgtcg
     7921 cgagctgaaa aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct
     7981 gacagtaggg cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga
     8041 cctgctgccg gtgggcgatt ttgcctggta cgatcatgta ctgaccacca gtttgctgct
     8101 gggtaatgtt ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt
     8161 ccgtattggt cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa
     8221 atggtttaac accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa
     8281 actgacctgg acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt
     8341 taagcctgtg ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca
     8401 gtttgaccgc cttagcctgc tgaacgacat tctgccggtt tatcagcaag tgctggcaga
     8461 actggcgaaa cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact
     8521 accacaggcg tggctggacg catacaaacc cgcttacgac gcgctccagg gacaggtgaa
     8581 actgctgctg accacctatt ttgaaggcgt aacgccaaat ctcgacacga ttactgcgct
     8641 gcctgttcag ggtctgcatg ttgatctcgt acatggtaaa gatgacgttg ctgaactgca
     8701 caagcgcctg ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg
     8761 gcgcgccgat cttaccgaga aatatgcgca aattaaggac attgtcggca aacgtgattt
     8821 gtgggtggca tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg
     8881 tcttgatgca gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactagc
     8941 actgctgcgc gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc
     9001 aattcaggcg cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc
     9061 ggcgatcacc gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca
     9121 gcgtgcacgt tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac
     9181 cacggaaatt cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaataacta
     9241 ccgcacgggc attgcggaac atatcaggca ggccattgtt gaacaggaac gtttgggact
     9301 ggatgtgctg gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca
     9361 cctcgacggg tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt
     9421 gaagccaccg attgttattg gtgacgttag tcgcccggca ccgattaccg tggagtgggc
     9481 gaagtatgcg caatctctga ctgataaacc ggtgaaaggg atgctgactg gcccggtgac
     9541 tattctctgc tggtcgttcc cgcgtgaaga tgtcagccgt gaaaccatcg ccaaacagat
     9601 tgcgctggcg ctgcgtgatg aagtggccga tctggaagcc gctggaattg gcatcatcca
     9661 gattgacgaa ccggctctgc gcgaaggttt accgctgcgt cgtagcgact gggatgcgta
     9721 tctccagtgg ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca
     9781 aatccacact cacatgtgtt attgcgagtt caacgacatc atggattcga ttgcggcact
     9841 ggacgcagac gtcatcacca tcgaaacctc gcgttctgac atggagttgc tggagtcgtt
     9901 cgaagagttt gattatccga atgaaatcgg tccaggcgtc tatgacattc actcgccaaa
     9961 cgtaccgagc gtggaatgga ttgaagcctt gctgaagaaa gcggcaaaac gcattccggc
    10021 agagcgcctg tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg
    10081 cgcggcactg gcgaacatgg tgcaggcggc gcagaatttg cgtcggggat gatgaatatt
    10141 aacgagtcac tgtaaattgc agtacgatga aagaggcaaa actgcctgat actaggccta
    10201 cataatttat gcaatttatt gaatttttgg gttttgttgg ccggacaagg cgttcacgcc
    10261 gcatccggca tgaacaacgc gcactttgcc aataatctga tggcttccat caccggaagc
    10321 cattttgcta tctgcgcccg ttcagataca gaaataaccg aatcattttt acgcaaaata
    10381 taatcctgag cgatttgttc agtaatcaac gctacgttat ttattctcac ttttcacgaa
    10441 attgtatccc ttttcagtaa cgtggtagtg aggcatagac ttatgtccgc tatcctcaat
    10501 aataaccaaa ccctcgttag ctaatttgtt aacaatgtta acaacattac cgaatcctgg
    10561 aatttgcctt atggatagtg tgcgatcgag attcatccag ttccagttta tatctttctc
    10621 agccataatt ttcagtacgt taatttcagt ttcagttatc atagtcaagt gcatcctggt
    10681 ggtataagtc attaagttct cccttaagtt tataatcttc tagagtagca gtatgcgta
//
LOCUS       DAZPBM010000141        10686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPBM010000141 DAZPBM010000000
VERSION     DAZPBM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.142"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHUZQ_003279"
     CDS             complement(<1..784)
                     /locus_tag="ACHUZQ_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1026898.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHUZQ_003280"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHUZQ_003280"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1026899.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHUZQ_003281"
     CDS             1860..2747
                     /locus_tag="ACHUZQ_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1026900.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHUZQ_003282"
     CDS             2780..3790
                     /locus_tag="ACHUZQ_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR1026901.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHUZQ_003283"
     CDS             3860..5293
                     /locus_tag="ACHUZQ_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1026902.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHUZQ_003284"
     CDS             5392..6741
                     /locus_tag="ACHUZQ_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1026903.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHUZQ_003285"
     CDS             6731..7618
                     /locus_tag="ACHUZQ_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1026904.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUZQ_003286"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUZQ_003286"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1026905.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHUZQ_003287"
     CDS             8352..9224
                     /locus_tag="ACHUZQ_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1026906.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUZQ_003288"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUZQ_003288"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1026907.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUZQ_003289"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUZQ_003289"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1026908.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2530 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZPBM010000142        10526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPBM010000142 DAZPBM010000000
VERSION     DAZPBM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.143"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHUZQ_003290"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHUZQ_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1026909.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="ACHUZQ_003291"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="ACHUZQ_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1026910.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="ACHUZQ_003292"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="ACHUZQ_003292"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1026911.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="ACHUZQ_003293"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="ACHUZQ_003293"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1026912.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5189..6229)
                     /locus_tag="ACHUZQ_003294"
                     /pseudo
     CDS             complement(5189..6229)
                     /locus_tag="ACHUZQ_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="ACHUZQ_003295"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="ACHUZQ_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1026913.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHUZQ_003296"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHUZQ_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1026914.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="ACHUZQ_003297"
     CDS             7164..7604
                     /locus_tag="ACHUZQ_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1026915.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHUZQ_003298"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHUZQ_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1026916.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHUZQ_003299"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHUZQ_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1026917.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..>10526
                     /gene="rsxC"
                     /locus_tag="ACHUZQ_003300"
     CDS             8833..>10526
                     /gene="rsxC"
                     /locus_tag="ACHUZQ_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1026918.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2404 a         2720 c         2693 g         2709 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccg
//
LOCUS       DAZPBM010000143        10430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZPBM010000143 DAZPBM010000000
VERSION     DAZPBM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.144"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUZQ_003301"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUZQ_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1026919.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUZQ_003302"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUZQ_003302"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1026920.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUZQ_003303"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUZQ_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR1026921.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUZQ_003304"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUZQ_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1026922.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUZQ_003305"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUZQ_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUZQ_003306"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUZQ_003306"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1026923.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUZQ_003307"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUZQ_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1026924.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUZQ_003308"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUZQ_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1026925.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLVVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHUZQ_003309"
     CDS             complement(9457..10149)
                     /locus_tag="ACHUZQ_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1026926.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2550 a         2516 c         2754 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccacc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAZPBM010000144        10303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPBM010000144 DAZPBM010000000
VERSION     DAZPBM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.145"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(5..2434)
                     /gene="hrpB"
                     /locus_tag="ACHUZQ_003310"
     CDS             complement(5..2434)
                     /gene="hrpB"
                     /locus_tag="ACHUZQ_003310"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1026927.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            2508..3038
                     /gene="thpR"
                     /locus_tag="ACHUZQ_003311"
     CDS             2508..3038
                     /gene="thpR"
                     /locus_tag="ACHUZQ_003311"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1026928.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            3053..3757
                     /gene="sfsA"
                     /locus_tag="ACHUZQ_003312"
     CDS             3053..3757
                     /gene="sfsA"
                     /locus_tag="ACHUZQ_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1026929.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            3935..4390
                     /gene="dksA"
                     /locus_tag="ACHUZQ_003313"
     CDS             3935..4390
                     /gene="dksA"
                     /locus_tag="ACHUZQ_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1026930.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            4457..5353
                     /gene="gluQRS"
                     /locus_tag="ACHUZQ_003314"
     CDS             4457..5353
                     /gene="gluQRS"
                     /locus_tag="ACHUZQ_003314"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1026931.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            5413..6810
                     /gene="pcnB"
                     /locus_tag="ACHUZQ_003315"
     CDS             5413..6810
                     /gene="pcnB"
                     /locus_tag="ACHUZQ_003315"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1026932.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            6807..7286
                     /gene="folK"
                     /locus_tag="ACHUZQ_003316"
     CDS             6807..7286
                     /gene="folK"
                     /locus_tag="ACHUZQ_003316"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1026933.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            7656..8240
                     /locus_tag="ACHUZQ_003317"
     CDS             7656..8240
                     /locus_tag="ACHUZQ_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1026934.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            8345..9085
                     /locus_tag="ACHUZQ_003318"
     CDS             8345..9085
                     /locus_tag="ACHUZQ_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1026935.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            9120..10303
                     /gene="htrE"
                     /locus_tag="ACHUZQ_003319"
                     /pseudo
     CDS             9120..10303
                     /gene="htrE"
                     /locus_tag="ACHUZQ_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         2569 a         2664 c         2518 g         2552 t
ORIGIN      
        1 aaccttacga atactttttc gtccgtcgcg tcggtgccgt gtttgccggg tcgtccggcc
       61 agacgtgttt gggataacgc cctttcatct ctttttgcac ctcacggtac gctcctttcc
      121 agaaggcgct caaatctcgt gtgatttgta gtggcctttg ggcgggtgaa agcaactcca
      181 gcaccagcgg cacgcccccc tgggcgatag tcggattagt ggcctcacca aacatctcct
      241 gcattctcac cgccagtgcg ggcgggttat cttcatgata acgaatggcg atccggcttc
      301 ccgtcggcac agtgtaatgc gcaggcaatt cactatccag acgttgctgc attccccaat
      361 caagtaatcc gcgtagtgcc tgataaatgt ccagtgattt caggccgcgt agtgaatgta
      421 cgcctgtcat atgtggcagc agccacgttt ccagcgctgc caataaactt tcatcatcaa
      481 ccgctggcca gtcatattcc ggcaaccact ttgcggcgca taacaaacgc aagcgtagct
      541 gttccgcttc cgccgtccag ttgagcacgc ttaaaccttt atcacggatg ccattaagca
      601 tcgcctgatg caactcgtct tctgacggtt tcgccagcgg ctgcactttc accgtcaact
      661 gaccgatttg tagccgacgc caggctttca gcgtaccttg cgcgtcatcc cattccacgg
      721 tgtcagattg ctgcaccagc tgcggacagc gttgtactaa ttcatcaata tcgaccggta
      781 gcgccagtaa aatccgcgca tccggcgagg cgctgccctg caataataac ggtgcgatca
      841 accattcgtg gcggcttagc gcgtcgtcgg catcgagcat cgctcccatg ccgtttgcca
      901 gttgatagcg tccatcttgc ccacggcgat gagcaatgcg atcggcaaac gccccggcaa
      961 gtagcggcgc gataagcgaa ctgtctgcct caccgccgcg tacgtttaag cgttttaaca
     1021 gttgctgact acgttgctgc caggctggtt gattgcgcga aaacgccacg cccaggtcac
     1081 tattgcccat cagtggcggc tcttcgagaa tggcggcaat ttttgccgcg gtagcagctt
     1141 cgtcgtcgtt cttcgcgcta accagcattg ccgctaaacg cggatcgtta cccagcgctg
     1201 ccattttttg cccttgcgca ctaagccgtt caccgtccag cgcccccagc atttgtaaca
     1261 gacgtttcgc ggccattaga ttcactactg gcggttgatc cagccagctc atctgcgccg
     1321 gatcgctgca tccccattgc agtaattcca tcagcaaacc ggaaagatcg ctttgtaaga
     1381 tctccggttc actttgcgcg gcagcgcgtt ctgcttgttc tttggcgatt aaatgcaggc
     1441 agatacccgg ctccagacgc ccggcgcgcc cggcacgctg cgtcatggat gcctggctaa
     1501 cgcgttgagt aatcagtcgc gtaagccccg tgcgcggctc aaaacgcgcc acacgctcct
     1561 gggcacaatc caccaccaga cgaatacctt caatggttaa actggtttca gcaatattgg
     1621 tcgccagcac cactttgcgc atcccttgcg gtgccgggag gatcgctttt cgctgatcgt
     1681 tcagcgacaa cgcgccatac agcgggcaga gcaacacatc actgccgatg cgcgaagcca
     1741 gttgttcctg cacacgctga atttctccga cgccaggtaa aaataacagt aatgatccgc
     1801 tttcctgacg cagcatttcg gcggtggcta ccgcaacggc ttcgtcaaaa cgctgatgcg
     1861 cgggcagcgg taaataatgg cgttcaaccg gaaacgagcg cccttctgag atgacgacgg
     1921 gcgcttctgg cagcatttgc tgcaagcggt cgttatccag cgtagccgac ataatcagca
     1981 gtttaaggtc atcacgcaga ccttgttgca catcgagtaa cagcgccaac gccaaatccg
     2041 cctgcaagct gcgctcatga aattcatcaa ggatcaccaa tccaacaccg ctcaactccg
     2101 ggtcacgctg gatcatccgc gtcagcacgc cttcggtaac tacttccaga cgggtatttg
     2161 gcccgacgca gttttgcgca cgcatccggt agccaacggt atcgcctggc ttttcgttaa
     2221 gcagctccgc cagccgttgc gcgacgttac gcgccgccag acgacgcggc tccagcagga
     2281 taattttccc gttaatgccg ggatgcgcca gcagttgcag cggcagccag gttgatttcc
     2341 cggccccggt cggcgcactt aataatacct gcggcgcaca atcgagagcg gtaagtaatt
     2401 caggtaagac ggcagcaacg ggcaacgacg acacaaaacg ctccagaggg ttaacattct
     2461 tcgcgccaca ttgtagcatc gcggtaattc ataaccgagt gcctcacatg tctgaaccgc
     2521 aacgtctgtt ctttgctatc gacttacctg cagaaatccg cgaacagatt atccactggc
     2581 gcgccacaca cttcccacct gaggcgggac gtccggtcgc cgccgataat ttgcatctga
     2641 ctctggcatt tttaggcgaa gtgagcgccg agaaagagaa ggcgctttct cttttagccg
     2701 gacggattcg tcaacctggt ttcacactca cgcttgatga cgccggacaa tggctgcgtt
     2761 cgcgtgtggt gtggttaggg atgcgtcagc cgccacgcgg cttaatccag ctggcgaata
     2821 tgctccgttc acaggctgcc cgcagcggtt gttttcaaag caatcgtccg tttcatccac
     2881 atattacctt attgcgcgac gccagcgagg cggtgacaat cccgccgcca ggttttaact
     2941 ggtcttatgc ggtgacggag ttcacccttt acgcctcctc gtttgcccgt ggacgcacac
     3001 gctacacgcc gctaaaacgc tgggcgctaa cgcaataaca aggattgtcg caatggaatt
     3061 ctctccccct ctacagcgcg cgacgctaat tcagcgttac aaacgttttt tagccgatgt
     3121 gatcacaccc gatggtcgcg aattaacgct acactgcccg aatacgggtg cgatgaccgg
     3181 ttgtgcaacg cgtggcgata ccgtctggta ttcgacttca gacaacacca aacggaaata
     3241 cccacacacc tgggaactaa ctcaaagcca gagcggcgca tttatttgcg tcaatacgct
     3301 ttgggctaac aggttgacga aagaggctat ccttaatgaa tcaatttcag aactgtcagg
     3361 ctatagctcg ctgaaaagcg aagtaaaata cggcgcagaa cgcagccgta ttgactttat
     3421 gttgcaggcg gattcgcgtc cagactgcta tattgaagtg aaatcggtta cgttagcgga
     3481 gaacgaacag ggatattttc ccgatgcggt cactgaacga ggtcagaaac accttcggga
     3541 gttgatgaac gtagcggctg aaggccagcg tgcggttatc tttttcgccg tgctgcattc
     3601 agccattaca cggttttcac ccgcgcgcca catcgatgag aaatacgcgc aactattgtc
     3661 agaagctcaa cagagggggg tagaaattct ggcttacaaa gcggaaattt ctgctgaagg
     3721 catggctctt aaaaaatcac tgtcggttac attgtagtaa agtaagtaac tggttaattt
     3781 acattctggt cgcgtgcgca aatacgcttt tcctcacaca gttgtcaagt gttacgttta
     3841 gataattgct atccggaaaa gcatctgcta tttatagcgg cctcattttt cccccgaaca
     3901 tggggatcga tagtgcgtgt taaggagaag caacatgcaa gaagggcaaa accgtaaaac
     3961 atcgtccctg agtattctcg ccatcgctgg ggtggaacca tatcaggaga agccgggcga
     4021 agagtatatg aatgaagccc agctggcgca cttccgtcgt attctggaag catggcgtaa
     4081 tcaactcagg gatgaagtcg atcgcaccgt tacacatatg caggatgaag cagccaactt
     4141 cccggacccg gtagaccgtg cagcccagga agaagagttc agcctcgaac tgcgtaaccg
     4201 cgatcgcgag cgtaagctga tcaaaaagat cgagaagacg ctgaaaaaag tggaagacga
     4261 agatttcggc tactgcgaat cctgcggtgt tgaaattggt attcgccgtc tggaagcgcg
     4321 cccgacagcc gatctgtgca tcgactgcaa aacgctggct gaaattcgcg aaaaacagat
     4381 ggctggctaa ttacagccgt tccatcacgt ttaccacagg cgggaaatgc tcccgcctta
     4441 ttttttgttc aaagagatga cagacacaca gtatattggc cgcttcgccc cctctccttc
     4501 cggcgagctt cattttggct ctctgatcgc cgcgctcggc agctatttgc aggctcgcgc
     4561 ccggcaaggt cgctggctgg tacgcataga agatatcgac ccgcctcgtg aagttcccgg
     4621 tgccgcagaa actatcctgc gccagctgga acattacggt ctgcactggg acggcgatgt
     4681 tctctggcaa tcgcaacgtc acgacgccta tcgtgaagca ctcgcctggt tacatgaaca
     4741 aggactaagt tattactgca cctgtacgcg tgcgcgtatt caaagcattg gcggtattta
     4801 cgacggtcat tgccgggtgt tgcatcatgg accagacaat gccgcagtgc gtatccgcca
     4861 gcagcatccg gtcacgcaat ttactgacca gctgcgcggc attattcacg ccgacgaaaa
     4921 actggcacgg gaagatttta tcattcatcg ccgtgatggg ttgttcgcct acaacctggc
     4981 tgttgtggtt gatgatcatt tccagggcgt tacagaaata gtgcgtgggg ctgatctgat
     5041 tgaaccaaca gtaaggcaaa tctcgctgta ccagcttttt ggctggaaag tgccagatta
     5101 cattcatctg ccgctggcgc ttaatccaca aggcgctaaa ctttccaagc agaatcatgc
     5161 gcctgcgttg ccgaaaggcg atccacgtcc ggtactaatc gcggcacttc aatttctggg
     5221 tcagcaggca gaagcacact ggcaggattt cagcgtcgaa caaatccttc agtcagcagt
     5281 caaaaactgg acgctgaccg ccgtgcccga gtcggcaatt gtaaattcaa cattctcaaa
     5341 tgcgtcatgc tgagctatga ttagccgcta tttttttgtc ctgaatgatg tttgacacta
     5401 ccgaggtgta ctatttttac ccgagtcgct aatttttgcc gcaaggtgct aagccgcgag
     5461 gaaagcgagg ctgaacaggc agtcgcccgt ccacaggtga cggtgatccc gcgtgagcag
     5521 catgctattt cccgcaaaga tatcagtgaa aatgccctga aggtaatgta caggctcaac
     5581 aaagcgggat acgaagcctg gctggttggc ggcggcgtgc gcgacctgtt acttggcaaa
     5641 aagccgaaag attttgacgt aaccactaac gccacgcctg agcaggtgcg caaactgttc
     5701 cgtaactgcc gcctggtggg tcgccgtttc cgtctggctc atgtaatgtt tggcccggag
     5761 attatcgaag ttgcgacctt ccgtggacac cacgaaggta acgtcagcga ccgcacgacc
     5821 tcccaacgcg ggcaaaacgg catgttgctg cgcgacaaca ttttcggctc catcgaagaa
     5881 gacgcccagc gccgcgattt cactatcaac agcctctatt acagcgtagc ggattttacc
     5941 gtccgtgatt acgttggcgg tatgaaggat ctgaaggacg gcgttatccg tctgattggt
     6001 aacccggaaa cgcgctaccg tgaagatccg gtacggatgc tgcgcgcggt acgttttgcc
     6061 gccaaattgg gtatgcgcat cagcccggaa accgcagaac cgatccctcg cctcgctacc
     6121 ctgctgaacg atatcccacc ggcacgcctg tttgaagaat cgcttaaact gctacaagcg
     6181 ggctacggtt acgaaaccta taagctgttg tgtgaatatc atctgttcca gccgctgttc
     6241 ccgaccatta cccgctactt cacggaaaat ggcgacagcc cgatggagcg gatcattgaa
     6301 caggtgctga agaataccga tacgcgtatc cataacgata tgcgcgtgaa cccggcgttc
     6361 ttgtttgccg ccatgttctg gtacccactg ctggagacgg cacagaagat cgcccaggaa
     6421 agcggcctga cctatcacga cgctttcgcg ctggcgatga acgacgtgct ggacgaagcc
     6481 tgccgttcac tggcaatccc gaaacgcctg acgacgttaa cccgcgatat ctggcagttg
     6541 cagttgcgta tgtcccgacg tcagggtaaa cgcgcatgga aactgctgga gcatcctaag
     6601 ttccgtgcgg cttatgacct gttggccttg cgagctgaag ttgaacgtaa cgctgaactg
     6661 cagcgtctgg tgaaatggtg gggtgagttc caggtttccg cgccaccaga tcaaaaaggg
     6721 atgcttaacg agttggatga agagccgtca ccgcgtcgcc gtactcgtcg tccacgcaaa
     6781 cgcgcaccgc gtcgtgaggg taccgcatga cagtggcgta tattgccata ggcagcaatc
     6841 tggcctctcc gctggagcag gtcaatgctg ccctgaaagc attaggcgat atccctgaaa
     6901 gccacattct tgccgtttct tcgttttacc gcactccacc gctggggccg caagatcaac
     6961 ccgattacct caacgccgcc gtggcgcttg aaacttcgct cgccccggaa gagttactca
     7021 accatacaca gcgcatagaa ctgcaacagg gtcgtgttcg taaagctgaa cgctggggac
     7081 cacgcacgct ggatctcgat atcatgctgt ttggtaatga agtgataaat accgaacgcc
     7141 tgaccgttcc gcactacgat atgaagaatc gtggatttat gctgtggccg ctgtttgaga
     7201 ttgcaccgga gctggcgttt cctgatgggg agacgttgcg tgaagtctta catacaagag
     7261 catttgacga attaagcaaa tggtaattta tttatattac gaatataaat attttctgaa
     7321 aatcatacat cctctctttc cttaataaaa attttagttt aaacattttt gcagaaacat
     7381 aatataaatt cccgcagata aatatcaatc ccatcgccat accctaacca attgcacttg
     7441 tgaatttatt tttgcttaat tttgaattaa gaaaatttaa tttgttcaaa agcccttaaa
     7501 gtgcacaata ggcgtcctaa tgaacaatgg ttgtaaattt tggtaacaaa tatcattgct
     7561 cattaatatc tagcatctgc ttataacatt tgtgtcatga atagtcattc ttcaaaccag
     7621 tagtctgact aatggaattt tattcaagga tttttatgtc taaaaaatta ggttttgccc
     7681 tgtctggtct tatgctggca atggttgcag gtactgcttc tgctgatatg gatggtggtc
     7741 agttaaatat cagtggtctg gttgttgata acacctgtga aacccgcgtt gacggcggta
     7801 ataaagatgg tctgatcctg ctgcaaactg caactgttgg tgaaatcgat gctggcgtgc
     7861 tgaatgacac cgttggcgct aaagccaaac ctttcagcat caccgttgac tgcagcaaag
     7921 caaatccgaa tccaggtagt acagctaaaa tgaccttcgg ttctgttttc tttggtaata
     7981 gcaaaggaac gctgaacaac gacatgtcta ttaacaaccc gtctgacggc gtgaatatcg
     8041 ctctgcacaa tattgatggt tccactatca aacaggttca aattaacaac cctggcgatg
     8101 tgtatactaa agccctggat gcaacgacaa aatctgctgt ttatgatttt aaagcgtctt
     8161 acgttcgtgc cgtcgcagac caaacagcaa ctgctggtta tgtaaaaact aacactgcat
     8221 acaccattac ttatcagtaa gtattcacca attaaattga ttgaatgaat atacagggaa
     8281 tagtgttaat aatttctatt tttatattat tccctgtttt aattaactct gtcagggatg
     8341 gtttatgttt tttaacacca aacatacaac agctttatgc ttcgtaacct gtatggcttt
     8401 tagttcatcg tctattgcgg acattgtcat ttcgggtact cgcgtaatat ataaaagcga
     8461 tcaaaaaagt gtcaacgtac gtctggaaaa taaagggaat aacccgttgc ttgtccagag
     8521 ttggttagat actggcgata acaacgctga gcctggcagt attacagtcc cttttactgc
     8581 cacgccgcca gtatcgcgta ttgatgccaa acgtgggcaa acaatcaaat taatgtacac
     8641 agccagcacc tcactgccta aagacagaga gagcgtgttc tggtttaacg tactggaagt
     8701 tccaccaaaa ccagatgcag aaaaggtcgc gaatcaaagt ctgctgcaac tggcatttcg
     8761 cacacgtata aaacttttct atcgcccgga tggattgaag ggaaatccct ctgaagcccc
     8821 gttagccctg aagtggttct ggtcaggttc agaaggtaag gcgtcattgc gagtgaccaa
     8881 tccaacccct tactacgtct cttttagcag tggtgattta gaagctagcg gtaaacgcta
     8941 tccgattgat gtgaaaatga ttgcaccatt tagtgatgag gtcatgaaag tcactggcct
     9001 taatggcaaa gcaagttctg caaaagtaca tttttacgcc attaatgatt ttggtggcgc
     9061 cattgaaggt aatgccagtc tgtaatcagg caaggatata attccgcagg aagcataacg
     9121 tgactataaa accaactaaa aattatcata atcatctgac ccgtatcgct acgttttgcg
     9181 cgctgctgta ttgcaatagt gctttctgtg ctgaacttgt tgaatatgac cataccttcc
     9241 tgatggggca gaatgcctca aatattgatc tcagccggta tagtgaaggt aaccctgcta
     9301 taccgggtat gtacgacgtc agtgtttatg tcaacgatca acctattatt aaccaaagca
     9361 ttacatttat tgaaattgaa ggaaaaaaaa cgcacaagcc tgtatcacac ttaagaattt
     9421 attgcagttt catataaatt ctcccgatat aaataacgaa aaagccgttc tgcttgccag
     9481 ggatgaaaca ctcggcaatt gccttaattt gacggaaatt atccctcagg cttctgttcg
     9541 ttatgacgtt aacgagcaac gtctggatat agacgttcct caggcatggg taatgaaaaa
     9601 ttaccaaaac tatgttgatc catcgttatg ggaaaatggc attaatgcgg ccatgttgtc
     9661 atacaacctc aacggatatc atagtgaaac tcctggtcga agaaatgaca gcatttatgc
     9721 tgcttttaac ggtgggatga atttaggtgc atggcgactg cgtgcctcag gcaactacaa
     9781 ctggatgacc gattctggca gtaattatga ttttaagaat cggtatatac agcgtgatat
     9841 cgcctcgctg cgttctcaac tcattcttgg tgagtcttat acaacgggcg aaacctttga
     9901 ttctgtcagt atccgcggca ttcgtttata cagcgacagc cgcatgttgc ctccgacatt
     9961 agcgagtttt gcgcctatca ttcatggcgt tgctaatacc aacgccaaag tcactattac
    10021 gcaaggtggc tataagattt atgaaacgac tgtgccgcca ggcgctttcg tcattgatga
    10081 tctgagtccg tcaggttacg gcagcgatct tattgttact gtcgaagaat ccgatggctc
    10141 aaagcggaca ttctcgcaac ctttttcatc ggtcgttcaa atgttacgcc ctggcgttgg
    10201 acgttgggat attagcggcg gccaggtctt aaaagatgat attcaggatg agcctaattt
    10261 atttcaggcc agctactact acggtctgaa taactatcta acg
//
LOCUS       DAZPBM010000145         9868 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPBM010000145 DAZPBM010000000
VERSION     DAZPBM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.146"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..348
                     /locus_tag="ACHUZQ_003320"
     CDS             <1..348
                     /locus_tag="ACHUZQ_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1026936.1"
                     /translation="NPWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGK
                     KHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETIL
                     SWLLVDPSSTAHE"
     gene            complement(332..970)
                     /gene="ytfB"
                     /locus_tag="ACHUZQ_003321"
     CDS             complement(332..970)
                     /gene="ytfB"
                     /locus_tag="ACHUZQ_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR1026937.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1189..1809
                     /gene="fklB"
                     /locus_tag="ACHUZQ_003322"
     CDS             1189..1809
                     /gene="fklB"
                     /locus_tag="ACHUZQ_003322"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1026938.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2118..3530
                     /gene="cycA"
                     /locus_tag="ACHUZQ_003323"
                     /gene_synonym="dagA"
     CDS             2118..3530
                     /gene="cycA"
                     /locus_tag="ACHUZQ_003323"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1026939.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3575..4237)
                     /gene="ytfE"
                     /locus_tag="ACHUZQ_003324"
     CDS             complement(3575..4237)
                     /gene="ytfE"
                     /locus_tag="ACHUZQ_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1026940.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4345..5310)
                     /locus_tag="ACHUZQ_003325"
     CDS             complement(4345..5310)
                     /locus_tag="ACHUZQ_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1026941.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5418..6278)
                     /gene="qorB"
                     /locus_tag="ACHUZQ_003326"
     CDS             complement(5418..6278)
                     /gene="qorB"
                     /locus_tag="ACHUZQ_003326"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1026942.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6367..6747
                     /locus_tag="ACHUZQ_003327"
     CDS             6367..6747
                     /locus_tag="ACHUZQ_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1026943.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6865..8808)
                     /gene="cpdB"
                     /locus_tag="ACHUZQ_003328"
     CDS             complement(6865..8808)
                     /gene="cpdB"
                     /locus_tag="ACHUZQ_003328"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1026944.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8998..9738
                     /gene="cysQ"
                     /locus_tag="ACHUZQ_003329"
     CDS             8998..9738
                     /gene="cysQ"
                     /locus_tag="ACHUZQ_003329"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1026945.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2293 a         2488 c         2558 g         2529 t
ORIGIN      
        1 aacccttggt ttttaaaaat tgtgcatagt gaaaatcaaa gtaaaggtgt gcattatgca
       61 aaatcactgc gtctcttaga aattaaccat gcgcacttgc agttaatgga atccttattg
      121 gatgaaggga agaaacacaa tatttttaag ccagatatcg acccattgca ggtgaatatt
      181 aatattgctg cgcttggggg atattatttg atcaaccagc atacgcttgg cctggtttat
      241 cacatcagta tggtttcacc ccaggcactg gaagccagac gtaaggtcat caaagagaca
      301 atccttagct ggcttttggt tgacccttca tctaccgcgc acgaataaaa ctgccatccg
      361 gctggcgggt aaacaggacc tgttgattat tccccgtatc aatggttaag cccgtcacca
      421 cgccgctggc gttctggcgg attttcacca tctgcccatt ttgcaaatta ctcagcggct
      481 tacccgcgcc ttctacctgc gccattgcat agacatcggt agcgggcagt ccgtggtcgc
      541 ggaacagctg cgccatcgtt ttaccgggtt cgacacggta agagcgccat tgattatcaa
      601 ttccgctatc tggctggaat ggttgcgttt gggtagtttg cggctgttct tctggctggc
      661 cttcctgtat aggttctgga gcgaccggag ccacctgatc aggatcattt tgtggtgtga
      721 ctaattgcgc ccgcagctgt tcttctgtcg gcggttgtga ttgcgactga atatctaact
      781 gcgcttcacg cgtcaccacc ggcgcgttgg gcgtatcatc agatggaagc agaaatccga
      841 ccaccaatac aatggcggca ataatgatcc cccgacgatg catcggcggc agcgggtcca
      901 tgaagcgaaa attgtccggc gcgtgccaga ctttctccag ggttggtttt agttcaaagc
      961 gcccgggcat ggtttcctcc tgctccgcgt cttgttcctc aatcatagcc tatgaataag
     1021 ctaacgctat gatgtccgtg gtaaacccgc ctttattata ttcatacggg atattgctat
     1081 tgtttctttt tccccgggat ttgtcatcat tcctgcgaca aagttttacc caaagaagtg
     1141 tggctgatat gctgcccgct acttttatac cctaagaaag gaaatacgat gaccacccca
     1201 acttttgaca ccatcgaagc gcaagcaagc tacggcattg gtttgcaggt agggcaacaa
     1261 ctgagtgaat ctggcctgga agggctgctg ccagaagcac tggttgcagg tattgccgat
     1321 gcgctggaag gcaaacatcc ggctgtgccg gttgatgtgg tgcatcgcgc gctgcgtgaa
     1381 atccacgaac gcgccgatgc cgttcgtcgt cagcgtttcc aggcgatggc tgctgaaggt
     1441 gtgaaatacc tggaagaaaa cgccaaaaaa gaaggtgtga atagcaccga atctggcctg
     1501 caattccgcg tgatcaacca gggtgaaggc gcaattccgg cacgtaccga ccgcgttcgt
     1561 gttcattaca ccggtaaact gatcgacggc accgtgtttg acagctccgt tgctcgtggt
     1621 gaacccgctg aattcccggt taatggcgtg atccctggct ggattgaagc actgactctg
     1681 atgccggtag gttctaaatg ggaactgact atcccgcagg aactggcata tggcgagcgc
     1741 ggcgcaggcg catccatccc tccgttcagc accctggtgt ttgaagtcga actgctggaa
     1801 atcctctaag cagcgcattc tgttcccctc gaaccagagg ggagcaggca ttcagcaata
     1861 aacccttcag tttgccaaac ggcgctattt tgtgttgcaa agaccccgta agcgtgtatt
     1921 tttgtgagct gtttcgcgtt atcaccgtga tatgacactc actttaaaca taaaattaac
     1981 attagatcta aatcttagta ttcatcccgc gtattgttac ctaatatcga tgagtcccga
     2041 tacagattcg tcgtatcata gactgactaa aggccgtaga gcctgaacaa cacagacagg
     2101 tacaggaaga aaaaaacatg gtagatcagg taaaagtcgt tgccgatgat caggctccgg
     2161 ctgaacagtc gctacggcgc aatctcacaa accgacatat tcagcttatt gccattggcg
     2221 gtgccattgg tacggggttg tttatggggt ccggcaaaac gattagcctt gccgggccgt
     2281 cgatcatttt cgtttatatg atcatcggtt ttatgctctt tttcgtgatg cgggcaatgg
     2341 gggaattgct gctttcgaat ctggaataca aatcttttag tgacttcgct tccgatttac
     2401 tcgggccgtg ggcaggatat ttcaccggct ggacttactg gttctgctgg gttgtaaccg
     2461 gtatggcaga cgtggtggcg atcactgctt atgctcagtt ctggttcccc gatctctccg
     2521 actgggtcgc ctcgctggcg gtgatagtgc tgctgctgac gctcaatctc gccaccgtga
     2581 aaatgttcgg tgagatggag ttctggtttg cgatgatcaa aatcgtcgcc attgtgtcgc
     2641 tgattgtcgt cggcctggtt atggtggcga tgcactttca gtcaccgact ggtgtggaag
     2701 cgtcattcgc gcatttgtgg aatgatggcg gctggttccc gaaaggttta agtggcttct
     2761 ttgccggatt ccagatagcg gttttcgctt tcgtagggat tgagctggta ggtacaaccg
     2821 ctgcggaaac caaagatccg gagaaatcac tgccacgcgc gattaactcc attccgatcc
     2881 gtatcattat gttctacgtc ttcgcgctga ttgtgattat gtccgtgacg ccgtggagtt
     2941 cggtagtccc ggagaagagc ccgtttgttg aactgtttgt gttggtaggt ttgcctgcgg
     3001 ctgccagcgt gatcaacttt gtggtgctga cctctgcggc gtcttccgct aacagcggcg
     3061 tcttctctac cagccgtatg ctgtttggtc tggcgcagga aggtgtggca ccgaaagcgt
     3121 tcgctaaact ttctaagcgc gcagtacccg cgaaagggtt gaccttctcg tgtatctgtc
     3181 tgctcggcgg cgtggtgatg ttgtatgtga atcctagcgt gattggcgcg ttcacgatga
     3241 ttacaaccgt ttccgcgatt ctgtttatgt tcgtctggac aattatcctt tgctcgtacc
     3301 tggtttaccg caaacagcgt cctcatctgc atgagaagtc gatctacaag atgccgctcg
     3361 gcaagctgat gtgctgggta tgtatggcgt tctttgtgtt cgtggtcgtg ttgctgacac
     3421 tggaagatga cactcgccag gcgctgttgg tcaccccgct gtggtttatc gcactggggc
     3481 tgggctggtt gtttattggt aagaagcggg ctgctgaact gcggaaataa ccgcattatc
     3541 atgctggatg gcgcaatgcc atccagcttt tagattactc acccgccagc gcgcgtggga
     3601 acagtacatt gttttccagg ctgatgtgat ccatcaggtc atcaatcagt tcattaatac
     3661 cgttatacat cgctttccag gtggtgcagg cttccggcgg cggtgtgacg ttattggtgg
     3721 tgtgtttaat cacttccagc agttcgcccg cttcatcgtg ctcgctttcc atcacgctga
     3781 ttggtcccat tgcctggctg cccatgcctt gtttgatcat cgggaagagg atctgctctt
     3841 ctttcatcat gtggctggat agctcttcat gcagcatagt caggtatttt gtcagccctt
     3901 ttggcacgct cggtttgtcg gcgtgaacgc gctcgacttt agtcgcttgc aggatcagct
     3961 ccggcagttg ctcgcggtga cgatcgtggt agcgcacgat gatatggtca atgatttctg
     4021 ccagcggggc gctacgccag tctttctcaa tcggttgttc agcaagcttt gccagttcag
     4081 cttcaatgac ctcaacatcc agttctttac gtgccgccgc gcgcgccagc gtttgcttac
     4141 cgccacagca gtaatccata tcatatttac gaaacagagc tgaagcgcga ggaatagaga
     4201 gcgccagttc acctaaaggt tggtcgcgat aagccatagc tgatacctca ttcttaataa
     4261 tataagatgt attttaaatg catctttaag gcaaaagcta taacccttac gtagcagagg
     4321 cttatttaac tcactgccag cgtgtcaact ttctgagaca gattcagtta aaggtttttc
     4381 tggttttgct ctgaccgcaa tgaccacgcc aatcactaac agcgcgatac cgctcagcgt
     4441 cattagcggc ggcatttgct ggcggagtaa aaaggtgtac agcaaacctg ccagcgtttc
     4501 gaaaacaatc agcggcccga gaatcactgt cggtaatcgc tggctggcga cgttccagca
     4561 gagtgcgcca acccatgagc aaagcacggc tatcgcaacc atcagactaa taaacaccag
     4621 cggacggggg ccaaaaggta aggagaagtc cggcgtttgt atattcagcc agtaacaggc
     4681 gacgagatag ccgatgagag aaaccggcag tgtgaccagc gcctgcgccg ttgcccacat
     4741 catcggatgt ttgtcgggat tttcccgcag ccagcgggcg ttgcgcaggg cataccacgc
     4801 ccagcagacc acggaaacta acgccagcac gatgccagag gtataacgtg cccagtcaaa
     4861 atcggggagt ccgtggttta actcagcaat attcacacac gccaggccga tgccaataca
     4921 aaccagtgcc ggggcgagtt ttccccatgc gagtttgccg tcgcgctggc tataaagcag
     4981 attggcaaaa acgggaatca ccaccggcag ggtgccgata atcatcgtgg aaacaggcgc
     5041 gccagtacgt tgaatggcac tggcaaggca gaagtaatag ataaggttgc ccatcatggt
     5101 gagcatcaag gcggtaagcc agtcgcgacg cgccaactga cgcagacgca cgcgtcccag
     5161 ccaggcaatg ggcagcgcaa ttaaccctaa cgccagataa cgccccatcg actgcaacat
     5221 cgccgggtat tccggtacga tcaacggccc gacaaaaata agcccccaca tcaaccctgc
     5281 taacagggcg tacagcacgc cgctaatcat tactggcatc cattggtctg tcagaagaaa
     5341 gttcaggaag cagacatgat tgctcccggc atcccgtcat tatcagagag gatgcgggag
     5401 gatgccactt taattaacta gttattaaca ttaaaaagat ggcttacgct ttcggctaac
     5461 gttgtcgttg ggcggccaat caatttgcta agcgttttgc tgttatcaaa cagaccacct
     5521 ttcgatgcgc caacgtcaga atccgccagc atatccgcca gtccgtcggg cagtccgacg
     5581 cttttcagtg ccgcggcgaa atcggcttcg ctcagatttt gataggtaac ctgtttgccg
     5641 ctctgtttgg tcagctctgc cgctaactgt gtcaacgtcc aggcactatc gcccgccagt
     5701 tcgtaaacct tgccttcgtg accggcttcg ctaatcacgc gtgccgcagc tgccgcataa
     5761 tctgcccgcg ttgctgaggc gattttgcca tcgcccgccg caccgataaa tacgccgtgt
     5821 tccagtgctg ccggggcgct ggcgaggtag ttttcgctgt accagccgtt gcgcagcagg
     5881 gtgtaaacga tgccagaatc agccagcatt ttctccgtct caatgtgctc atcggcgagg
     5941 ccgagcgggg aggtatctgc atgtagcagg ctggtataag cgataaattt cacgccagcc
     6001 gcctttgcgg cattaataac attacgatgc tgcggggcac gttgacccac ttcgctggaa
     6061 gagatcagca gtagtttttc cactccctga agtgcagatg tcagtgcggc ttcatcgccg
     6121 tagtcagcct gacgcacggt aatgccttgt gctgctagag cctgggcttt tgccggatta
     6181 cgaacgatag ccactatttg gctggcagga accgttttca tcaaggattc aataatatag
     6241 tgaccaagtt ggccagtggc accagtaata gcgatcatgg gaagtctcca tcgttttgtc
     6301 ttgtgtttaa atacgctaac acctaaactt acttttagta agtacgtaca aaaaggtaag
     6361 tatgaaatga gtcaggttag cctgtcgcaa caactgaaag agggtaatct ctttgcggaa
     6421 cagtgcccgt cgcgcgaggt gttgaaacac gtcaccagcc gttggggggt gttgattctg
     6481 gtggcgctac gcgaaggtac tcatcgcttt agcgacctgc ggcgcaaaat cggcggggtg
     6541 agcgaaaaga tgcttgcgca gtcgttacag gcgttagaac aggatggttt tcttaaccgt
     6601 atcgcgtatc cggtggtgcc accgcatgtt gaatacagcc tcacgccgct gggcgagcag
     6661 gtgagcgaaa aggttgctgc actggcggac tggattgagt tgaatttgcc cgaggtgttg
     6721 gcggtgcggg atgaacgtgc ggcataactg ccggatgcgc ggcctacggt gtatgacttg
     6781 tagggcggat aagatgcgcc agcatcgcat ccgccaataa atgccggatg cgattgatga
     6841 tttataggcc gaaaaagaag tgttttactt actcaaatcc acctgataaa tcgcaaaccc
     6901 gatatcatcg gtcgcgactt tattcatcgg atactgccct ttctctttaa taaacgctgc
     6961 ggctttattt gacggggaag tttcgaaacg gatatccagt ttcttatcgg cagctatcgg
     7021 tgctaaacgc cagttgttat ctgccgccgg gtgaatttcc cccgcacgtt tcgactcatc
     7081 agcaatccac gctgccagca ccgagcggtt ctcatccggt gaagcaaaag cgatatggct
     7141 gtcgcccgta ccggcaaatt tgccgccgta agcgcgatag ttattggtgg caacgaggaa
     7201 catggcgttc ggatcaatcg gcttgccatt aaaggtcagg ttcttaatcc tttccgcatt
     7261 ggtattaatc atctggcact cgccgtcata acgggcgggc tgggtaacat caatctgata
     7321 attcacacca tcaataacat caaagttata agtgcggaaa ccatcccagt tgatgagtga
     7381 ctgtggtttc gtgctgttag gatcaatctg gttaaactgt ccggcggagc actccagcca
     7441 ctctttcacc tctttaccgc tggctttcac tacaatcagc gtattggggt agagataaag
     7501 atcggcggca ttacggaagg tcaactggcc tttttccacc tccacatagc ttgccgggtc
     7561 atttttgcga ccaccaactt taaacggtgc ggcagctgaa agcaccggca gttttgccag
     7621 atccggatcg ccctgaatgt aatgttcgac atacgctttt tgcgcgttgt tcacgacttg
     7681 cacggtcgga tcgtcctgca ccagcgccag atagctatac atattgtcgg cagatttacc
     7741 gattggcttg ctgacgaact ggcgtgtggc atcgtgatcg gctttgagtg tttctaccag
     7801 cttgctgtct tccgccgcga gggatttttt attggcgatg tcgtaaatcg gccgagcttc
     7861 cgctttcgcc tgcgtcacct gccatttacc gctgtcatta ctgagttgta agtcgaccac
     7921 cccaagatga tcgccccaca tgcccggcat taccgccgga acaccattca gcgtgccttt
     7981 ggtgatatca gccccttcga tatcagcaaa atctttaccc gggaaaacgg cgtgagcatg
     8041 accaaacata atggcgttaa cgcccggaat ttcactgagg taataaactg agttttccgc
     8101 catcacttta tacggatcgg cagacagccc ggaatgtgcc agaacgacaa cgacatcggc
     8161 acctttctcg cgcatttcag gcacgtattt gcgcacggtt tcggtaatat cattcaccgt
     8221 cactttcccg gataaattag ctttatccca gcccatgatt tgcggcggca cgacgccaat
     8281 atagccaatc ttcagcgtct gtttttttcc gtctttatcg accacttcgg tatctttaat
     8341 taaatacggt gtaaacattg gctgtttggt tctggcgtca atgacgttgg catttacata
     8401 agggaatttc gctcccgcca gcgcattttt caggtaatcc agaccgtagt taaattcatg
     8461 attgccgagt gtaccgaccg tataatccag cgtattcagc gccttataaa ccggatgaac
     8521 atcacctgtt tttaatcctt tcgccgacat gtaatctgcc agcggactcc cctgaatcaa
     8581 atcgccgtta tcgaccagta cgctgttttt cacttcattg cgggcatcgt taatcaggct
     8641 tgccgtacgt accagtccga atttttccgt ggcggtgtct ttgtaataat cgaaatccat
     8701 catgttgcta tgcagatcag tggtttccat gatacgcaga tcgaccgtcg ctgcattcac
     8761 actggcggca atcagcgtgg ccaggagcgt tgcgctaaac ttaatcatca gggacatcct
     8821 tttatcatcg ggaatacgaa agaaaaggga gaataaacgt cttacttata gaacagtgaa
     8881 gaatgccaca attttacgct ttgaaaatga tgacactatc acagttggcg cattcattaa
     8941 cgatagggta taagtaaaac aataagttaa caccgctcac agagacgagg tggagaaatg
     9001 ttagatcaag tatgccagct tgcacggaat gcaggcgatg ccattatgca ggtctacgac
     9061 gggacgaaac cgatggacgt cgtcagcaaa gcggacaatt ctccggtaac ggcagcggat
     9121 attgccgcac acaccgttat catggacggt ttacgtacgc tgacaccgga tattccggtc
     9181 ctttctgaag aagatcctcc cggttgggaa gtccgtcagc actggcagcg ttactggctg
     9241 gtagacccgc tggatggtac taaagagttt attaaacgta atggcgaatt caccgttaac
     9301 attgcgctca ttgaccatgg caaaccgatt ttaggtgtgg tgtatgcgcc ggtaatgaac
     9361 gtaatgtaca gcgcggcaga aggcaaggcc tggaaagaag agtgcggtgt gcgcaagcag
     9421 attcaggtcc gcgatgcgcg cccgccgctg gtggttatca gccgttccca tgcagatgcg
     9481 gagctgaaag agtatctgca acagcttggc gaacatcaga ccacgtccat cggctcttcg
     9541 ctgaaattct gcctggtggc ggaaggacag gcgcagctgt acccgcgctt cggaccaacg
     9601 aatatttggg acaccgccgc tggacatgct gtggctgcag ctgccggagc gcacgttcac
     9661 gactggcagg gtaaaccgct ggattacact ccgcgcgagt cgttcctgaa tccggggttc
     9721 agagtgtcta tttactaaat tcagatggca gaaacagtgt atttcctgat tctgccatcc
     9781 tgatttctcc caacctaaaa agttataaat aaaaagagat tgtatttaaa gtgcaaaaat
     9841 tcaattgcta ataagttaca ttttaata
//
LOCUS       DAZPBM010000146         9830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPBM010000146 DAZPBM010000000
VERSION     DAZPBM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.147"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            501..2132
                     /gene="oppA"
                     /locus_tag="ACHUZQ_003330"
     CDS             501..2132
                     /gene="oppA"
                     /locus_tag="ACHUZQ_003330"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1026946.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2218..3138
                     /gene="oppB"
                     /locus_tag="ACHUZQ_003331"
     CDS             2218..3138
                     /gene="oppB"
                     /locus_tag="ACHUZQ_003331"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1026947.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3153..4061
                     /gene="oppC"
                     /locus_tag="ACHUZQ_003332"
     CDS             3153..4061
                     /gene="oppC"
                     /locus_tag="ACHUZQ_003332"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1026948.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4073..5086
                     /gene="oppD"
                     /locus_tag="ACHUZQ_003333"
     CDS             4073..5086
                     /gene="oppD"
                     /locus_tag="ACHUZQ_003333"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1026949.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5083..6087
                     /gene="oppF"
                     /locus_tag="ACHUZQ_003334"
     CDS             5083..6087
                     /gene="oppF"
                     /locus_tag="ACHUZQ_003334"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1026950.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTFKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6140..6469)
                     /locus_tag="ACHUZQ_003335"
     CDS             complement(6140..6469)
                     /locus_tag="ACHUZQ_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1026951.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6504..7964)
                     /gene="cls"
                     /locus_tag="ACHUZQ_003336"
     CDS             complement(6504..7964)
                     /gene="cls"
                     /locus_tag="ACHUZQ_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1026952.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            8107..8280
                     /locus_tag="ACHUZQ_003337"
     CDS             8107..8280
                     /locus_tag="ACHUZQ_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1026953.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8335..9588)
                     /gene="kch"
                     /locus_tag="ACHUZQ_003338"
     CDS             complement(8335..9588)
                     /gene="kch"
                     /locus_tag="ACHUZQ_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1026954.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
BASE COUNT         2585 a         2285 c         2436 g         2524 t
ORIGIN      
        1 tagaggaaag tgctaaataa taatcaattg ttaaattatt gtgcatttca ctactggaac
       61 tgtaatcaga aaagatagac atgcttagcc aatctctatt tgattgaatt gaaagatgtt
      121 tgttaaggca tggatgcaag ctatagattc tgatacggtc aataaaagag aattgcttaa
      181 caattttgca aaatgtattg gcgagtaaga accgcatttg gtactttccg ggcaaccgcc
      241 agacgattct ttattggtaa tgagaataat taacaattaa agagcgtcgc gaaagaataa
      301 tgtgtctcga caggggagac acagtacgaa tcgacataag gtgatcgtct gaatcaccag
      361 aataaataaa gtcggtgata gtaatacgta acgataaagt aacctgacag cagaaagtct
      421 ccgagcctgt gcagggtccc aatccgggat tacacatgct ggttaatacc agtaattata
      481 atgagggagt ccaaaaaaca atgaccaaca tcaccaagag aagtttagta gcagctggcg
      541 ttctggctgc gctaatggca gggaatgtcg cgctggcagc tgatgtaccc gcaggcgtca
      601 cactggcgga aaaacaaaca ctggtacgta acaatggttc agaagttcag tcattagatc
      661 cgcacaaaat tgaaggtgtt ccggagtcta atatcagccg agacctgttt gaaggcttac
      721 tggtcagcga tcttgacggt catccagcac ctggcgtcgc tgaatcctgg gataataaag
      781 acgcgaaagt ctggaccttc catttgcgta aagatgcgaa atggtctgat agcacgccag
      841 tcacagcaca agactttgtg tatagctggc aacgttctgt tgatccgaac actgcttctc
      901 cgtatgccag ttatctgcaa tatgggcata tcgccggtat tgatgaaatt cttgaaggga
      961 aaaaaccgat taccgatctc ggcgtgaaag ctattgatga tcacacatta gaagtcacct
     1021 taagtgaacc cgttccgtac ttctataaat tacttgttca cccatcaact tcaccggtgc
     1081 caaaagccgc tatcgagaaa ttcggcgaaa aatggaccca gcctggtaat atcgtcacca
     1141 acggtgccta taccttaaaa gattgggtcg taaacgaacg aatcgttctt gaacgcagcc
     1201 cgacctactg gaacaacgcg aaaaccgtta ttaaccaggt aacctatttg cctattgctt
     1261 ctgaagttac cgatgtcaac cgctaccgta gtggtgaaat cgacatgacg tataacaaca
     1321 tgccgatcga attgttccag aagctgaaaa aagagatccc ggacgaagtt cacgttgatc
     1381 catacctgtg cacttactat tacgaaatta acaaccagaa accgccattc aacgatgtgc
     1441 gtgtgcgtac cgcactgaaa ctaggtatgg accgcgatat cattgttaat aaagtgaaag
     1501 cgcagggcga catgcccgcc tatggttaca ctccaccgta tactgatggc gcaaaattga
     1561 ctcagccaga atggtttggc tggagccagg aaaaacgtaa cgaagaagcg aaaaaactgc
     1621 tggctgaagc gggttatacc gcagacaaac cgttgaccat caacctgctg tataacacct
     1681 ctgatctgca taaaaagctg gcgattgctg cctcttcatt gtggaagaaa aacattggtg
     1741 taaacgtcaa actggttaac caggagtgga aaacgttcct cgacacccgt caccagggca
     1801 cttttgatgt ggcccgtgca ggctggtgtg cagactacaa cgaaccaact tccttcctga
     1861 acaccatgct gtcgaacagc tcgatgaata ccgcgcatta taagagcccg gcctttgaca
     1921 gcattatggc ggaaacgctg aaagtgactg acgaggcgca gcgcacagct ctgtacacta
     1981 aagcagaaca acagctggat aaggattcgg ccattgttcc tgtttattac tacgtgaatg
     2041 cgcgtctggt gaaaccgtgg gttggtggct ataccggcaa agatccgctg gataatacct
     2101 acacccggaa tatgtacatt gtgaagcact aatggcaata cgtggggcag gagtgtcctg
     2161 ctccacggtg tctgattttt atcgcattac agaaggcaca ggccagaagg tagggcaatg
     2221 ttaaaattta ttctacgtcg ctgtctggaa gcgattccga cgctatttat tcttattact
     2281 atttcgttct ttatgatgcg cctcgcgccg ggaagccctt ttaccggcga acgtacttta
     2341 ccgccggaag tgatggccaa tatcgaagcg aaatatcatc ttaatgatcc gatcatgaca
     2401 cagtatttca gctacctgaa acaactggcg cacggtgatt tcggtccatc gtttaaatat
     2461 aaagattatt cggtcaatga cctggttgca tccagttttc ccgtttctgc caaactggga
     2521 gccgccgcat ttttccttgc ggtaatactg ggtgttagtg ctggcgttat tgtcgcatta
     2581 aaacaaaaca ccaaatggga ctataccgtg atggggctgg caatgaccgg ggttgttatc
     2641 cccagttttg tggttgcgcc attattagtc atgatatttg cgatcatttt gcattggctg
     2701 ccgggcggtg gctggaatgg tggggcgctt aaattcatga tattgccgat ggtggcgttg
     2761 tcactcgctt atatcgccag tattgcgcgt attacccgag gctctatgat tgaagtatta
     2821 cactccaact ttattcgtac tgcccgggcg aaagggttac ctatgcggcg gatcatttta
     2881 cgccacgcat taaaacctgc tctgttaccc gtgctctctt atatgggccc cgcatttgtc
     2941 ggcattatta ccggttctat ggttatcgaa accatttatg gtttgccggg gattggacaa
     3001 ctgttcgtta atggcgcatt gaaccgtgac tattccttag tgttaagcct gaccatcctg
     3061 gttggtgctt taaccatttt gtttaatgcc attgtcgatg tgctatatgc ggttatcgac
     3121 ccgaaaatcc gttactgata ctggagctcg cgatgatgtt aagtaagaaa aacagcgaga
     3181 cgctggaaaa tttcagtgaa aagctggagg tcgaagggcg cagcttgtgg caggacgcac
     3241 gtcgacgttt tatgcataac cgtgcggcgg ttgccagtct gatagtgctg gtgctgatcg
     3301 cgttatttgt aatcctggca ccgatgcttt cgcagtttgc ctatgacgat actgactggg
     3361 cgatgatgtc cagcgccccg gatatggagt ccggtcacta ctttggtact gactcatccg
     3421 gtcgcgacct gcttgtgcgc gttgcgattg gcgggcgtat ctcactcatg gtcggtgttg
     3481 ctgcggcact ggtggcagtg gtcgtgggga cactttacgg ttcgctttcc ggttatctgg
     3541 gcggtaaagt ggattcggta atgatgcgtc tgctggaaat cctcaactcc ttcccattca
     3601 tgttcttcgt cattttgctt gtgacctttt tcggtcaaaa catcctgctg attttcgtgg
     3661 cgattggcat ggtttcctgg ctggatatgg ctcgtattgt gcgtggacaa accctgagtc
     3721 tgaagcgcaa agagtttatt gaggcggcac aagttggcgg tgtatcgaca tcgggcattg
     3781 ttattcgcca cattgtgccg aatgtactcg gtgtggtggt ggtctacgca tcacttctgg
     3841 tgcccagcat gatcctcttt gaatctttcc ttagcttcct ggggttgggt acgcaagagc
     3901 cgttaagcag ctggggtgca ttgctgagtg atggcgcgaa ctcgatggaa gtctctccat
     3961 ggttattgtt gttcccagcg ggattcctcg tggtgacgct gttttgtttc aactttatcg
     4021 gcgatggctt gcgtgatgct ctcgacccga aagatcgtta aggagtgcag ccatgagcgt
     4081 aattgaaact gcaactgtgc cgctcgcaca acaacaggct gacgcactgc tgaacgtgaa
     4141 agatttgcgt gtcaccttta gtaccccgga cggcgatgtc acggcggtca atgatttgaa
     4201 tttttctcta cgtgccggag aaacgctggg cattgtaggt gagtctggtt cgggtaaatc
     4261 gcaaactgca tttgcgttga tgggcctgtt ggctgccaac ggacgtattg gcggatcggc
     4321 aaccttcaat gggcgtgaaa tcctcaattt gccagagcgt gaactcaata aactgcgcgc
     4381 tgaacaaatc tcaatgattt ttcaggaccc aatgacttcg ttgaatccct atatgcgcgt
     4441 cggtgagcag ttgatggaag tgctgatgct gcataagaac atgagcaaag ctgaggcgtt
     4501 tgaagagtcg gtgcggatgc tcgatgcggt aaaaatgccg gaagcacgta aacgcatgaa
     4561 aatgtacccg cacgaatttt ctggtggcat gcgtcagcga gttatgattg cgatggcatt
     4621 gctatgtcga cctaagctgc tgattgcgga tgaacccact actgcgctgg acgtcaccgt
     4681 acaggcgcag atcatgacgc tattaaatga actgaagcgg gaatttaata ccgccatcat
     4741 tatgattacc cacgatcttg gcgtggtggc ggggatctgc gacaaagtgc tggtaatgta
     4801 cgccgggcgc acgatggaat atggcaacgc gcgcgatgtc ttttatcaac ccgttcatcc
     4861 ttattctatc ggtttgctca acgcggtgcc gcgtctcgat gcggaaggtg aaacaatgtt
     4921 gaccatccct ggtaatccgc caaacctgct gcgattaccg aaaggttgcc cgttccagcc
     4981 acgttgtccg catgcgatgg aaatttgtag tagcgcaccg ccgctggaag agtttacgcc
     5041 tggccgtctg cgtgcttgct ttaaaccggt ggaggaactg ttatgaatgc tgtaaccgaa
     5101 ggaagaaaag tcctcctcga aatcgccgat ctgaaagtgc actttgaaat caaagatggc
     5161 aaacagtggt tctggcaacc gccgaaaact ttcaaagccg tcgatggtgt cacgcttcgc
     5221 ctgtatgaag gggaaacatt aggtgtggta ggggaatcgg gatgcggtaa gtccaccttt
     5281 gctcgcgcca tcattggcct ggttaaagcg accgacggtc atgttgcctg gttaggtaaa
     5341 gagttgctgg gcatgaagcc cgatgaatgg cgtgccgttc gcagtgatat tcagatgatt
     5401 ttccaggatc cgttggcatc gctaaacccg cgtatgacca tcggcgagat catcgctgaa
     5461 ccactgcgta cttatcatcc gaaaatgtca cgccaggaag ttcgcgagcg cgtgaaggcg
     5521 atgatgctga aagtcgggtt attgcctaac ctgattaacc gctatccgca tgagttctcc
     5581 ggtgggcagt gccagcgtat cgggattgct cgtgctctta ttcttgaacc gaagctgatt
     5641 atctgcgatg agccgatttc ggcgctggac gtgtcaattc aggcgcaggt ggtcaacctg
     5701 ctccagcagc tgcaacgtga gatgggattg tcattaattt ttatcgctca tgacctggcc
     5761 gtggtaaaac acatttccga tcgtgtgttg gtgatgtatc tcggccatgc ggtagaactg
     5821 gggacctatg atgaggtcta ccacaatcca ctacatcctt acaccagggc attgatgtcg
     5881 gcagtcccca tacctgatcc ggatctggag aagaacaaaa ccatccagtt actggaaggg
     5941 gaattaccgt cgccgatcaa cccgccttcc ggttgtgttt tccgtacctg ttgcccgatt
     6001 gccggtccgg agtgcgccaa aacacgtcct gtgctggaag ggagtttcag acacgccgtt
     6061 tcttgcctga aagtcgatcc gctttaaaca caataagggc tgacagttgt cagccctttt
     6121 tcacgccgaa agcgattatt tattcccgcc agatgatatg gcaaagtttg tgatcttttt
     6181 cgcggcataa cagaatgcgg gcgaaaacat cgttgatttc accatcctca ctgtccgcca
     6241 gaccaatcac cacttcggca aaaaagtccg ggttcaaatc gaaatcaaca tgttcctgcc
     6301 agtcttccgc cggatcgaat aactccgcgc cgccgcgctc ttcaaactga agattgaaca
     6361 gcagaacatc agctgggtcg agattgtcgg cagccagttc gagaaaaata tcgtaggcct
     6421 gctcaagcgt ttcatcttca gtcaggcgat tgtttagatc catatccata gtcactacct
     6481 gtttaacctc tgttggcgac gttttacagc aacgggctga agaagtaaaa cagtcgctcg
     6541 gcgacacgtt gccataatgg acgttttagc cataaacggg catcgagcag acgtgaacgc
     6601 gaaatataat cgtcctgaac ggcggcaagg tctgcaccaa aacctttatc gtcgattgcc
     6661 agggtaatct cgaaattcag ccacagacta cgcatatcaa ggttaacggt gccaaccaga
     6721 cttagttcgc catcgaccag cacgctcttg gtatgcagta acccgccttc aaactgataa
     6781 attttaaccc cagcagcaag cagttccgta aagaatgcgc gactggccca gccgaccagc
     6841 atcgagtcat tttttcgcgg aaggataata ctgacatcca ccccgcgctg cgccgccgtg
     6901 caaatcgcat gaagtaaatc atcgcttggc acaaagtagg gcgttgtcat gatcaaatat
     6961 tcacgcgccg aataagccgc agtcaataat gcctggtgaa tgagatcttc cggaaagccg
     7021 gggccagaag caattgtgtg aatggtgtga ccgctggcct gttcaaacgg cataatattg
     7081 acatctggtg gtggcggcag aatacgtttt ccggtttcaa tctcccagtc gcaggaataa
     7141 ataatcccca tcgcggtggc gatggggcct tccatacgcg ccatcagatc aatccattgc
     7201 cctacgcccg catcttgttt gaagtagcga ggatcgacca tattcatgct gccggtgtac
     7261 gcgatgtaat tatcgatcat gatcatcttg cgatgttggc gcaggtccat acggcgtaaa
     7321 aacacacgca tcagattgac ctttaaggct tcgaccactt caataccggc attacgcatt
     7381 agctcgggcc acgggctgcg gaaaaaagcc acactcccgg cggagtcgag catcaatcgg
     7441 caatgaatac cacgccgtgc agccgccatt aatgattcag ccacctggtc cgccatgccg
     7501 ccgggctgcc agatataaaa caccatctca atattatggc gcgcgagctg gatgtcgcgg
     7561 attaacgcct gcatcacatc atctgactcg gtcatcagtt gtagctgatt ccctttgacc
     7621 ccagcgatcc cctgacgacg ctcgcaaagc ttgaataatg gcgcagcgac actgctattt
     7681 tcttcggcga agatatgctt acaggcttta aggtcgttaa gccattttgc ggtggaaggc
     7741 cacatcgctc tggcgcgctc agcgcggcgt ttgcctaaat ggagctcgcc aacggcaaga
     7801 taggcaataa ttccgactaa cggcagaatg taaataatca acagccaggc catcgcggag
     7861 ggaactgcgc gtcgtttcat tagaatgcgt aaagttacgc ctgcaatgag caaccagtat
     7921 cccagaatgg ccaaccaact caccaacgta taaacggttg tcatagatta gaaatccttt
     7981 tgaaagcgca ttgttatgag tttacgcata tggattcatc tggcaaataa aaacgcggga
     8041 aaagcactgg tcagcgtcgg gggtgggggt ataatgacca ttctgttatt gcatagagta
     8101 gttaacatga agcggagtag aacggaagtg gggcgctggc gcatgcagcg tcaggctagc
     8161 cgacgtaaat cgcgttggct tgaggggcaa tcgcgccgaa atatgcgtat ccacagcatc
     8221 aggaagtgca ttctaaacaa acagcgtaac tcgttattgt ttgcgatcta caatatctaa
     8281 atatactggg caccattgcg gtgcccaata agtttactct ttcgtcttta aactctattt
     8341 ttgcactgat tctttactgt cagctttcgt ttcttgttca tcgttatcgc tgaaaatgcc
     8401 atgaccggag ttcaacaaca ttgaaacgag catatcatta ttaatctctt caccatttaa
     8461 aactcgcgcc agaatttcgc tgccaaacag ttgcggtgag agaatgatat ccggatgtac
     8521 catcttaatc ttattcaggt ttttgctatc actgacggcg agaactgttt tgacatcact
     8581 gctcatatct tttgccgaga gtacaacaaa cgcgttatct gcatcgttat cactcagcgc
     8641 cagaatggcc cggcaacgat cgattcccgc tttctttaat actgaactgt cattactgtc
     8701 accggggata acatcagcgt tatcgcctaa gcgttgctca agttgcttga tatcatcttc
     8761 aggcaagttg ctgataaccg ttacgttttg tccgcgttga ttcagttgca ggatcgtatt
     8821 gatggcgaga atcgaatgtc cgcaaacaat aaaatgatct ttacgatgca ttgtatgatt
     8881 gtttcctttt acaagtttgt tgaatccccc gcggataagc ggaccaaaaa ttgaggtcat
     8941 agatgtggca aaaacggtaa tgccggaaat aatgaccgaa atagtgaaca atcgtgctga
     9001 ttcagaaaca gggacaatat cgccatagcc gacggttgac atggtttcta tcgaaaaata
     9061 gaacgcggtc atcaaacttt ctattcgcgg attaaaacct tcgcttaaat aaagcgcacc
     9121 gtaggttgag taaaacagta atgtcgtgaa actaataaat gcaaaaattg tcccggctgc
     9181 ggcgctactg tgggagaagt ctttgcgcag tatcagcaaa aacaccagcg taaaaatgca
     9241 aaatccaata ctaaatttca gccagggata aaaatgcagg gtgtaaatta gcgctatcaa
     9301 caacaaaatg atactgattg cccatgcgag ctttgcccgg aacagtaagc caatggagtt
     9361 caggacaaga aagacgccga gcataaatag tggggcgttt gccagcaagg agagatccaa
     9421 ctctgaaaat gctttgatat ggaaaatatc aagcaaattg accgacatac catagattgt
     9481 tttaaaaata agcaatccat ttaaaaagac ggccagcgcg agaatgtcgt gccgaagggt
     9541 cacccataaa tttgttgctg tttgtttgaa tgtagcccag tgactcactg atacctctgt
     9601 aaccacaatt ttgtttaatt atcttagcgt gcacattttc tgaacataca tgcagcgcgt
     9661 gaatgtgtta aacaatagta gatgacttta tccgcagtaa tcaaaggatt tgagcgagca
     9721 actgtacctc aggaaaggtc atttttcctg aatatgctca catcatataa agaaatacag
     9781 ataaagttat tatctgcttg tggtagtgaa tgcactgacc ggctataagg
//
LOCUS       DAZPBM010000147         9757 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPBM010000147 DAZPBM010000000
VERSION     DAZPBM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.148"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..196)
                     /locus_tag="ACHUZQ_003339"
     CDS             complement(<1..196)
                     /locus_tag="ACHUZQ_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026955.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYN"
     gene            274..1368
                     /gene="ddlA"
                     /locus_tag="ACHUZQ_003340"
     CDS             274..1368
                     /gene="ddlA"
                     /locus_tag="ACHUZQ_003340"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1026956.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1392..1604)
                     /locus_tag="ACHUZQ_003341"
     CDS             complement(1392..1604)
                     /locus_tag="ACHUZQ_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1026957.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1864..2172
                     /locus_tag="ACHUZQ_003342"
     CDS             1864..2172
                     /locus_tag="ACHUZQ_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1026958.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2231..3325)
                     /gene="yaiW"
                     /locus_tag="ACHUZQ_003343"
     CDS             complement(2231..3325)
                     /gene="yaiW"
                     /locus_tag="ACHUZQ_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR1026959.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3338..4558)
                     /gene="sbmA"
                     /locus_tag="ACHUZQ_003344"
     CDS             complement(3338..4558)
                     /gene="sbmA"
                     /locus_tag="ACHUZQ_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1026960.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4910..6067
                     /gene="ampH"
                     /locus_tag="ACHUZQ_003345"
     CDS             4910..6067
                     /gene="ampH"
                     /locus_tag="ACHUZQ_003345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1026961.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6068..6691)
                     /gene="iprA"
                     /locus_tag="ACHUZQ_003346"
     CDS             complement(6068..6691)
                     /gene="iprA"
                     /locus_tag="ACHUZQ_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR1026962.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6779..9667)
                     /locus_tag="ACHUZQ_003347"
     CDS             complement(6779..9667)
                     /locus_tag="ACHUZQ_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1026963.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNNKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2514 a         2407 c         2343 g         2493 t
ORIGIN      
        1 tattataaat aaaagttatg aaatatattc tcttcgttaa cttataatta agagaaaaaa
       61 actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc atttttgtga
      121 tctcgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac gctttgtcag
      181 cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta
      241 tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt
      301 tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat
      361 gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca agggcaatgg
      421 cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg
      481 cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac
      541 gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg
      601 ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt
      661 tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg tctgctacgc
      721 gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc
      781 agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc ggctaatcag
      841 ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat tgccgtcgat
      901 ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgagatc
      961 gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga gatcgtactc
     1021 accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta
     1081 gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc cgttcaggct
     1141 tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac
     1201 gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag tatgtatccg
     1261 aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt gattgagctg
     1321 gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata ttttaacgcc
     1381 cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaacccc
     1441 gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt
     1501 gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac
     1561 gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg
     1621 aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa cattttcgta
     1681 attgttaata aatttaactt aaggttgcca ttttgtaact cacaacgaag attcacgctt
     1741 cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt
     1801 cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt
     1861 tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct
     1921 tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg
     1981 caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat gcaggtccag
     2041 gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt cgggctgatc
     2101 ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca ctggcgacat
     2161 cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct ggcatgacat
     2221 tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc
     2281 aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt
     2341 cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc ttgtacagcg
     2401 ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt tcgctgtcgc
     2461 tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggttctttac
     2521 tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgactg
     2581 cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaagcggt
     2641 atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc
     2701 cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac ggataccctt
     2761 tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg cgcaccggat
     2821 taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg tcaaaaatcg
     2881 cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt ttgccgtttg
     2941 gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc cgttcagcac
     3001 gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat
     3061 aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca
     3121 gcccctgact ttcaaaggtg atggcgagat ctttcgccca ggcatcgcgg tctttcacgc
     3181 tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg ccctttttca
     3241 acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg
     3301 aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt
     3361 cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca
     3421 tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac
     3481 caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca acaattgacg
     3541 gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatgt
     3601 tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg ctaaacagct
     3661 cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac
     3721 ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg atccctacca
     3781 ctgccagcca tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg
     3841 gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc agcaccggca
     3901 ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc tccagcgttg
     3961 aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata tgacgcagtt
     4021 gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acatagtgac
     4081 tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc cccagaaaga
     4141 cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt agcgcggttt
     4201 gaatcaaatc atagaacggt gcataccagg cgttgacggc gaccccgact tccaccaaaa
     4261 accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc caacggtgcg
     4321 ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa
     4381 aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc
     4441 cggtgatacg cgccacccag tctcccccac cggcttgcca gaagataacg gcaatcaatg
     4501 cccaaacaaa ggccgagaga aaaaacgctc ccggctttgg gaaaaaagac ttaaacatat
     4561 gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccacta
     4621 caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct tagttcgcca
     4681 gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt acataagcgg
     4741 attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac
     4801 gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga
     4861 gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag
     4921 tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg cggcgcagcc
     4981 gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta
     5041 cggcagcggt gcaacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg
     5101 tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt cggtcgtgcg
     5161 tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc tcgaccaggg
     5221 gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtgccgac
     5281 ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg ccctgccccg
     5341 tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc gcgagcaacg
     5401 ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg cggcgtactc
     5461 taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca agccttatac
     5521 ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca cctacacccc
     5581 ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa
     5641 cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata tgatgcgctg
     5701 gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag atcgcatgca
     5761 gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg tccccggcaa
     5821 agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat
     5881 tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga tcccgcagaa
     5941 gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag
     6001 cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc
     6061 atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt
     6121 cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa cgcgatatgc
     6181 gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct tcattccagt
     6241 caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca atgagctgca
     6301 cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga
     6361 gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat cccgtacaat
     6421 ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca
     6481 taatataagg ggcctgggta ataccgataa gtacgttttc ttctctacgc agagagataa
     6541 ctccctctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat ataacttgct
     6601 tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa
     6661 attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca
     6721 aatccttgac gggcattatt tagattaaat taccagtatt tcttcggagt gaagaatatt
     6781 accaggtata tttaacaccc acgttcgcgg accagtcttg atctacgtca ccaccaccga
     6841 ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga gtacccagac
     6901 caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc acatcgttat
     6961 cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga gcctgatctt
     7021 cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg ctgtcgtaag
     7081 actgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac tggaacagac
     7141 cagaaacgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag tcgtaaccgg
     7201 ctttcaagcc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa gtaccgttgc
     7261 tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagctacca tcaacaaaga
     7321 cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt ctggctgtct tgatctacct
     7381 gaccagaacg gtcattcatg tcacctttag cgaagcctgc agccgcaccg acgatccact
     7441 tagcgttgtt accgtcaact ttggtatcaa caccgaccat gatgccgtta acatcctgat
     7501 catagttgat ggtgccgttg tcgccgttga agttaccacc gaagtagctt acccatgcgc
     7561 cgccgttatc agccaggcca tgacgagagt tggtcagacg agtaccaacg gtgtcttgtt
     7621 ccaggttcca gatattggtg ttcgcagacg ggatgctcag cgccatgtta gcgtagtcgg
     7681 tcagctccat ctgttgcaga acaacggtgt taccgcgctg ttcagcctga taggtgtatg
     7741 cacccaggtc agctttgtta gcagcagaga aggtcgcgtt ggttttggta tcatcgatat
     7801 agatgagctg cttacctgcg taatcagcaa tcgcaccagc gccagtggag ttgtcgatac
     7861 gaactttgtt gttaccggtt gcagctacaa cgttgtctgc agagctgttg tcaacttcgt
     7921 cgccattgtt aatgtcgttg ttaccattaa cggtcaagtg accatcagag ttcatcgcaa
     7981 taacaccata accgtagtta gctttgctgg tgtcgttggt acgatcgttg acgaggtctg
     8041 cattcaggat gtaatcatta ctatgaatgt caaacacacc tgcgtaaaca ttaccgttgt
     8101 tatctttaac gttgccactg gtcagttcca gagtatcagt tgcaaatacg ctggagatgt
     8161 cattcatgtt ggtgtttaca tccagcacac caccgttagt aacggtaatg gtattggcat
     8221 acagcggtgc atatgcccaa gctgaagcag tatcttcacc gaagttaacg gttgagaagg
     8281 tatcaatggt caggtgatcg gtgttaactt caccgttaac ttccattgaa gaaccaccgt
     8341 tcagagtgat ggtgtcagaa gtcagagaag aatctgcact cacgttaacc tgagaaccgc
     8401 tgttgatcgc cagggtatcg atcagggaag ttttcgtggt ttcccaatca gaaccgttgt
     8461 tcagaacaac atcgaatacg ccgctctgga atgcgttagc gttatcaaca cgattccagc
     8521 catcttcaga gtaagaagcg tacgggttca ggctgtttgg tgcaacgtcg tagaaggctt
     8581 cgtcagcaac aacaacgtca taatcaatat tcgctgaacc aacccatttg gtgttgttca
     8641 gagtcaggtt caggtgatcg acgttctgat cgtcagttgc ccaaccgcca tgagtgtaag
     8701 cagtggtaga gttgtcaatc aggtgacccg tcgcgtcaaa gttagcgttc caggtactgt
     8761 caaatacaac gtcaccaagc aactgagagt tgttgaacgt aacgttgttg accatacgat
     8821 aatcagaagt gtcatcatcg atgaaggcta acgcaacatc atcagcagga gatgcgccat
     8881 tgccgtagtc acttggttca acagaattgc cgaaatgacc acgatctgat tgttcgtctt
     8941 cagtccatga accagaagtt acagtagagt cattaacaac gatgttgttg tcccactgat
     9001 gaccttcggt attgtaggtc gcattaccga ggtcgtgttg aaccaaagta atacctgcaa
     9061 catgggagtt gttgctgatg ttgatgttac tttcaacatc cagagtcaca gcggtaccaa
     9121 gaccagcggt taccaaacga tcataatctg tcttagatgt tttaccgtct gcatccagat
     9181 aagtatcggt gaagtagaag gcgtcaaaat catcgtcaat agtagaatta gcgatgttca
     9241 gagtgaagat gtcgccatca acccagtcgt ggccagtata atcgctaccg ttggtccaga
     9301 cgtagtcgcc ataaccaacc tggtaagatg taaccatacc atgaacaatt gagttggaga
     9361 tgttcagtgt attaccggta tctgtgccat aatcgtggtt cagataataa gtagaaatta
     9421 caccatttac agtgtcgccg ctgaaagtgg tatcaaagta accatcagct gcgtggtcat
     9481 atacataacc accgtaagtc agataatcgt gatcaatttt agtgctgtca ccccaattgt
     9541 agatggtggc tgcgtcatcg tgatacccga aggtgttata agtagctgca ttagcctgag
     9601 aggtgatagc cagagtgcaa gccaatgcta attgtgatac tacaagtttc tttttccagg
     9661 agtgcatttg tcatccctcc tcagggacgt aataagttga tccatcaatt atcaatgcat
     9721 agaaaacata ttatgtttcg cgctaacgat tatgctg
//
LOCUS       DAZPBM010000148         9739 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPBM010000148 DAZPBM010000000
VERSION     DAZPBM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.149"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(1..876)
                     /gene="espX1"
                     /locus_tag="ACHUZQ_003348"
                     /pseudo
     CDS             complement(1..876)
                     /gene="espX1"
                     /locus_tag="ACHUZQ_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(1361..1624)
                     /gene="rpsT"
                     /locus_tag="ACHUZQ_003349"
     CDS             complement(1361..1624)
                     /gene="rpsT"
                     /locus_tag="ACHUZQ_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1026964.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            1727..1945
                     /locus_tag="ACHUZQ_003350"
     CDS             1727..1945
                     /locus_tag="ACHUZQ_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1026965.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            1953..2894
                     /gene="ribF"
                     /locus_tag="ACHUZQ_003351"
     CDS             1953..2894
                     /gene="ribF"
                     /locus_tag="ACHUZQ_003351"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1026966.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            2937..5753
                     /gene="ileS"
                     /locus_tag="ACHUZQ_003352"
     CDS             2937..5753
                     /gene="ileS"
                     /locus_tag="ACHUZQ_003352"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1026967.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            5753..6247
                     /gene="lspA"
                     /locus_tag="ACHUZQ_003353"
     CDS             5753..6247
                     /gene="lspA"
                     /locus_tag="ACHUZQ_003353"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1026968.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            6372..6821
                     /gene="fkpB"
                     /locus_tag="ACHUZQ_003354"
     CDS             6372..6821
                     /gene="fkpB"
                     /locus_tag="ACHUZQ_003354"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1026969.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            6823..7773
                     /gene="ispH"
                     /locus_tag="ACHUZQ_003355"
                     /gene_synonym="lytB"
     CDS             6823..7773
                     /gene="ispH"
                     /locus_tag="ACHUZQ_003355"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1026970.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            7839..8753
                     /gene="rihC"
                     /locus_tag="ACHUZQ_003356"
     CDS             7839..8753
                     /gene="rihC"
                     /locus_tag="ACHUZQ_003356"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1026971.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            8920..>9739
                     /gene="dapB"
                     /locus_tag="ACHUZQ_003357"
     CDS             8920..>9739
                     /gene="dapB"
                     /locus_tag="ACHUZQ_003357"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1026972.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         2315 a         2301 c         2643 g         2480 t
ORIGIN      
        1 gatcgttaag ttcatacatc tccagtgatt tattataggg taataaaagt ataaattcct
       61 ttatattatc tccgcagtaa gaaaagacga cattaaattt attagtggcc atgttttcag
      121 ttgagcagta agctgatttt tctattaacc gactgggcac gcttttaaat aaagataatt
      181 ttccacatag ttttgaatat gccgaattgc tagttttaaa gttctgccac attattccct
      241 ggttatcatc tatcgatgag gaggggattt tattgcattg ctcattgtat agctcaggga
      301 aaatattttt tttaataagc tttttgattt cctggttaag atgacttttt ttcttgtcta
      361 ttgcactgta ttttgcgttt tgtgacgttt cctgatgatc ataaaatgac tggagatttc
      421 tgcaggcctc ataaaatatt tcaggagtat atagctgtgg gtatatttct tggttgaagt
      481 ttgcctttaa aaaattaacg aatttttcat ggcattcttg gtatatctta atctcttcag
      541 aaagacattc gcttgttctg tcatcaaaaa catccaatct aagtaatgtg atacctttgt
      601 gtttaaaaaa taaaattgaa ctgtatgttc tatgagttta catctataaa aatatgcatc
      661 tctcagatga gtatattgaa aaaaatgatg aaggactgga gatggtttag gcaagttatg
      721 gcttctaaaa aagtcatcaa acaaaattaa gaactctgtt ttatactcgc aaaatacttt
      781 tactaactca ttttgtcttg ttttatttat tattacatca tctactatat ttgatttata
      841 aagacaatgt ggcgaagttg agataccatt tgtcattatc agtccttgaa gttgatgaat
      901 ttttattaat ttaatttatg agtgaatgtg actactatca aaaatagctc attaatttta
      961 tgaagtagaa tataaatagg tatttaacct gtgttgattg ttgttaatta tttacaagat
     1021 ctaataagga gatatttatg aatggttaaa gtgtatagga atttaattca aaaatagagt
     1081 ggtcttaagt ttaatacctc aatgtgtatc acaatatcca cattctttgt aggggagtct
     1141 ggagattgag tagatattct tgttcagaat gtatcagccg atggttctac gattcttaag
     1201 ccacgaagag ttcagaaagt gtaacggcat gtctgcaaaa gccagcatct tttgactatc
     1261 tggcaaccgg tagtgtgttc tctcacgcat cataaaagca gcaggcataa aaaaaacccg
     1321 cttgcgcggg ctttttcaca aagcttcagc aaattggcga ttaagccagt ttgttgatct
     1381 gtgcagtcag gttagcctta tgacgtgcag ctttgttttt gtggatcaga cctttagcag
     1441 cctgacggtc cacgatcggt tgcatttcgt taaatgcttt ctgtgcagca gctttgtcgc
     1501 cagcttcgat agctgcgtat actttcttga tgaaagtacg catcatagag cgacggcttg
     1561 cgttgtgctt acgagccttt tcagactgaa tggcgcgctt cttagctgat ttgatattag
     1621 ccaaggtcca actcccaaat gtgttctata tggacaattc aaaggccgag gaatatgccc
     1681 ttttagcctt cttttgtcaa tggatttgtg caaataagcg ccgttaatgt gccggcactc
     1741 gttacgtagt gatggcgcag gattctacca gcttgcgggg tgtgaataca gcttttccgc
     1801 gataaaaatt gcagcaggcg gtcactttct tcccgtgatt tgcgccatgg caatgaaaag
     1861 ccacttcttt ctgatttcgg tactcaatcg ccggttaacc ttgaccgctg tacaaggtat
     1921 actcggacga ttttcactgt tttgagccag acatgaagct gatacgcggc atacataatc
     1981 tcagccaggc cccgcaagaa gggtgtgtgc tgactattgg taatttcgac ggcgtgcatc
     2041 gcggtcatcg cgcgctgtta cagggcttgc aggaagaagg gcgcaagcgc aacttaccgg
     2101 tgatggtgat gctttttgaa cctcaaccac tggaactgtt tgctaccgat aaagccccgg
     2161 caagactgac ccggctgcgg gaaaaactgc gttaccttgc agagtgtggc gttgattacg
     2221 tgctgtgcgt gcgtttcgac aggcgtttcg cggcgttaac cgcgcaaaat ttcatcagcg
     2281 atcttctggt gaagcatttg cgcgtaaaat ttcttgccgt aggggatgat ttccgctttg
     2341 gcgctggtcg tgaaggcgat ttcttgttat tacagaaagc tggcatggaa tacggcttcg
     2401 atatcactag tacgcaaact ttttgcgaag gtggcgtgcg tattagcagc accgctgtgc
     2461 gtcaggcgct ggcggatgac aatctggctc tggcagaaag tttactgggg cacccgtttg
     2521 ccatctccgg gcgtgtagtc cacggtgatg aattagggcg cactataggt ttcccgacgg
     2581 cgaatgtacc gctacgccgt caggtttccc cggtgaaagg ggtttatgcg gtagaagtgc
     2641 tgggcctcgg tgaaaagccg ttacccggcg tggcaaacat cggaacacgc ccaacggttg
     2701 ccggtattcg ccagcagctg gaagtgcatt tgttagatgt tgcaatggac ctttacggtc
     2761 gccatataca agtagtgctg cgtaaaaaaa tacgcaatga gcagcgattt gcgtcgctgg
     2821 acgaactgaa agcgcagatt gcgcgtgatg aattaaccgc ccgcgaattt tttgggctaa
     2881 caaaaccggc ttaagcctgt tatgtaatca aaccgaaaca cggaaccgag aatctgatga
     2941 gtgactataa atcaaccctg aatttgccgg aaacagggtt cccgatgcgt ggcgatctcg
     3001 ccaagcgcga accgggaatg ctggcgcgtt ggactgatga tgatctgtac ggcatcatcc
     3061 gtgcggctaa aaaaggcaaa aaaaccttca ttctgcatga tggccctcct tatgcgaatg
     3121 gcagcattca tattggtcac tcggttaaca agattctgaa agacattatc gtgaagtcca
     3181 aagggctttc cggttatgac tcgccgtatg tgcctggctg ggactgccac ggtctgccga
     3241 tcgagctgaa agtagagcaa gaatacggta agccgggtga gaaattcacc gccgccgagt
     3301 tccgcgccaa gtgccgcgaa tacgcggcga cccaggttga cggtcaacgc aaagacttta
     3361 tccgtctggg cgtgctgggc gactggtcgc acccgtacct gaccatggac ttcaaaactg
     3421 aagccaacat catccgcgcg ctgggcaaaa tcatcggcaa cggtcacctg cacaaaggcg
     3481 cgaagccagt tcactggtgc gttgactgcc gttctgcgct ggcggaagcg gaagttgagt
     3541 attacgacaa aacttctccg tccatcgacg ttgcttttca ggcggtcgat caggatgcac
     3601 tgaaagcaaa atttgccgta agcaacgtta acggcccaat ctcgctggtg atctggacca
     3661 ctacgccgtg gactctgcct gcgaaccgcg caatctctat tgcacctgat ttcgactatg
     3721 cgctggtgca gatcgacggt caggccgtga ttctggcgaa agatctggtt gaaagcgtaa
     3781 tgcagcgtat cggcgtgacc gattacacca ttctcggcac ggtaaaaggt gcggagcttg
     3841 agttgctgcg ctttacccat ccgtttatgg gcttcgacgt tccggcaatc ctcggcgatc
     3901 acgttaccct agatgccggt accggtgccg ttcacaccgc gcctggccac ggcccggacg
     3961 actatgtgat cggtcagaaa tacggcctgg aaaccgctaa cccggttggc ccggacggca
     4021 cttatctgcc gggcacttat ccgacgctgg atggcgtgaa cgtcttcaaa gcgaacgaca
     4081 tcgtcgttgc gctgctgcag gaaaaaggcg cgctgctgca cgttgagaaa atgcagcaca
     4141 gctatccgtg ctgctggcgt cacaaaacgc cgatcatctt ccgcgcgacg ccgcagtggt
     4201 tcgtcagcat ggatcagaaa ggtctgcgtg cgcagtcact gaaagagatc aaaggcgtgc
     4261 agtggatccc ggactggggc caggcgcgta tcgagtcgat ggttgctaac cgtcctgact
     4321 ggtgtatctc ccgtcagcgc acctggggcg taccgatgtc actgttcgtg cacaaagaca
     4381 cggaagagct gcatccgcgt actctcgaac taatggaaga agtggcaaaa cgcgttgaag
     4441 ttgacggcat ccaggcgtgg tgggatcttg atgcgaaaga gatcctcggc gatgaagctg
     4501 atcagtacgt gaaagtgccg gacacattgg atgtatggtt tgactccgga tctacccact
     4561 cttctgttgt tgacgtgcgt ccggaatttg ccggtcacgc agcggacatg tatctggaag
     4621 gttctgacca gcaccgtggt tggttcatgt cttccctaat gatctccacc gcgatgaaag
     4681 gcaaagcgcc gtatcgtcag gtactgaccc acggctttac cgtggatggt cagggtcgca
     4741 agatgtctaa atccatcggc aataccgttt cgccgcagga tgtgatgaac aaactgggcg
     4801 cggatattct gcgtctgtgg gtggcatcaa ccgactacac cggtgaaatg gccgtttctg
     4861 acgagatcct gaaacgtgct gccgatagct atcgtcgtat ccgtaacacc gcgcgcttcc
     4921 tgctggcaaa cctgaacggt tttgatccag caaaagatat ggtgaaaccg gaagagatgg
     4981 tggtactgga tcgctgggcc gtaggttgtg cgaaagcggc acaggaagac atcctcaagg
     5041 cgtacgaagc atacgatttc cacgaagtgg tacagcgtct gatgcgcttc tgctccgttg
     5101 agatgggttc cttctacctc gacatcatca aagaccgtca gtacaccgcc aaagcggaca
     5161 gtgtggcgcg tcgtagctgc cagactgcgc tgtatcacat cgcagaagcg ctggtgcgct
     5221 ggatggcacc aatcctctcc ttcaccgctg atgaagtgtg gggctacctg ccgggcgaac
     5281 gtgaaaaata cgtcttcacc ggtgagtggt acgaaggcct gtttggcctg gcagacagtg
     5341 aagcgatgaa cgatgcgttc tgggacgagc tgttgaaagt gcgtggcgaa gtgaacaaag
     5401 tcattgagca agcgcgtgcc gacaagaaag tgggtggctc gctggaagcg gcagtaacct
     5461 tgtatgcaga accggaactg tcggcgaaac tgaccgcgct gggcgatgaa ttacgatttg
     5521 tcctgttgac ctccggcgct accgttgcag actataacga cgcacctgct gatgctcagc
     5581 agagcgaagt actcaaaggg ctgaaagtcg cgttgagtaa agccgaaggt gagaagtgcc
     5641 cacgctgctg gcactacacc caggatgtcg gcaaggtggc ggaacacgca gaaatctgcg
     5701 gccgctgtgt cagcaacgtc gccggtgacg gtgaaaaacg taagtttgcc tgatgagtca
     5761 atcgatctgt tcaacagggc tacgctggct gtggctggtg gtagtcgtgc tgattatcga
     5821 tctgggcagc aaatacctga tcctccagaa ctttgctctg ggggatacgg tcccgctgtt
     5881 cccgtcgctt aatctgcatt atgcgcgtaa ctatggcgcg gcgtttagtt tccttgccga
     5941 tagcggcggc tggcagcgtt ggttctttgc cggtattgcg attggtatta gcgtgatcct
     6001 ggcagtgatg atgtatcgct cgaaggccac gcagaagcta aacaatatcg cttacgcgct
     6061 gattattggc ggcgcgctgg gcaacctgtt cgaccgcctg tggcacggct tcgttgtcga
     6121 tatgatcgac ttctacgtcg gcgactggca cttcgccacc ttcaaccttg ccgatactgc
     6181 catctgtgtc ggtgcggcac tgattgtgct ggaaggtttt ttgccttcta gagcgaaaaa
     6241 acaataataa accctgccgg atgcgatgct gacgcatctt atccggccta cagattgctg
     6301 cgaaatcgta ggccggataa ggcgtttacg ccgcatccgg caaaaatcct caaatataag
     6361 agcaaacctg catgtctgaa tctgtacaga gcaatagcgc cgtcctggtg cacttcacgc
     6421 taaaactcga cgatggcacc accgccgagt ctacccgcaa caacggtaaa ccggcgctgt
     6481 tccgcctggg tgatgcttct ctttctgaag ggctggagca acacctgttg gggctgaaag
     6541 tgggcgataa aaccaccttc tcgttggagc cagatgcggc gtttggcgtg ccgtcaccgg
     6601 acctgattca gtacttctcc cgccgtgaat ttatggatgc aggcgagcca gaaattggcg
     6661 caatcatgct ttttaccgca atggatggca gtgagatgcc tggcgtgatc cgcgaaatta
     6721 acggcgactc cattaccgtt gatttcaacc atccgctggc cgggcagacc gttcattttg
     6781 atattgaagt gctggaaatc gatccggcat tggaggcgta acatgcagat cctgttggcc
     6841 aacccgcgtg gtttttgtgc cggggtagac cgcgctatca gcattgttga aaacgcgctg
     6901 gccatttacg gcgcaccgat atatgtccgt cacgaagtgg tacataaccg ctatgtggtc
     6961 gatagcttgc gtgagcgtgg ggctatcttt attgagcaga ttagcgaagt accggacggc
     7021 gcgatcctga ttttctccgc acacggtgtt tctcaggcgg tacgtaacga agcaaaaagc
     7081 cgcgatttga cggtgtttga tgccacctgt ccgctggtga ccaaagtgca tatggaagtc
     7141 gcccgcgcca gccgtcgtgg cgaagagtcg attctcatcg gccacgccgg gcatccggaa
     7201 gtggaaggta caatgggcca gtacagtaac ccggaagggg gaatgtatct ggtcgaatcg
     7261 ccggacgatg tgtggaaact gacggtcaaa aacgaagaga agctctcctt tatgacccag
     7321 accacgctgt cggtggatga cacgtctgat gtgatcgacg cgctgcgtaa acgcttcccg
     7381 aaaattgtcg gtccgcgcaa agatgacatc tgctacgcca cgactaaccg tcaggaagcg
     7441 gtacgcgccc tggcagaaca ggcggaagtt gtgttggtgg tcggttcgaa aaactcctcc
     7501 aactccaacc gtctggcgga gctggcccag cgtatgggca aacgcgcgtt tttgattgac
     7561 gatgcgaaag atatccagga agagtgggtg aaagaggtta aatgcgtcgg cgtgactgcg
     7621 ggcgcatcgg ctccggatat tctggtgcaa aatgtggtgg cacgtttgca gcagctgggc
     7681 ggtggtgaag ccattccgct ggaaggccgt gaagaaaaca ttgttttcga agtgccgaaa
     7741 gagctgcgtg tcgatattcg tgaagtcgat taagtcatta gcagcctaag ttatgcgaaa
     7801 atgccggtct tgttaccggc attttttatg gagaaaacat gcgtttacct atcttcctcg
     7861 atactgaccc cggcattgac gatgccgtcg ccattgccgc cgcgattttt gcccccgaac
     7921 tcgacctgca actgatgacc accgtcgcgg gtaatgtctc ggttgagaaa accacccgca
     7981 acgccctgca actgctgcat ttctggaatg cggagattcc gctcgcccag ggagccgctg
     8041 tgccgctggt acgcgcaccg cgtgatgcag catctgtgca cggcgaatcg ggaatggctg
     8101 gctacgactt tgttgagcac aaccgaaagc caatcgggat accagcgttt ctggcgattc
     8161 gtgatgccct gatgcgtgca ccagagcctg ttaccctggt ggccattggc ccgttaacca
     8221 atattgcgct gttactttca caatgcccgg aatgtaaacc gtatattcgt cgtctggtga
     8281 tcatgggcgg ttcttctggt cgcggcaact gtacaccaaa tgccgagttt aatatcgctg
     8341 ccgatccaga agcggctgcc tgtgtcttcc gtagcggaat cgaaatcgtc atgtgcggtc
     8401 tggatgtcac caatcaggca atattaaccc ctgactatct cgctactttg ccggagttga
     8461 atcgcaccgg aaaaatgctt catgcactgt ttagccacta ccgtagcggc agtatgcaaa
     8521 gcggtctgcg aatgcacgat ctctgcgcca tcgcctggct ggtgcgcccg gacttattca
     8581 ccctcaagcc gtgttttgtc gcggtagaaa ctcaaggcga atttacctca ggcacgacgg
     8641 tggttgatat cgacggttgc ctgggcaaac cagccaatgt aaaggtggca ttggatctgg
     8701 atgtgaaagg cttccagcag tgggtggctg aggtgctggc tctggcgtcg taacctgtca
     8761 catgttatcg gcatgcagtc attcatcgac tcatgccttt cactgatatc cctccctgtt
     8821 tatcattaat ttctaattat cagcgttttt ggctggcggc gtagcgatgc gctggttact
     8881 ctgaaaacgg tctatgcaaa ttaacaaaag agaatagcta tgcatgatgc aaacatccgc
     8941 gttgccatcg cgggagccgg ggggcgtatg ggccgccagt tgattcaggc ggcgctggca
     9001 ttagaaggcg tgcagctggg cgcggcgctg gagcgcgaag gatcttcttt actgggcagc
     9061 gacgccggtg agctggctgg tgcagggaaa acaggcgtta ccgtgcaaag cagcctcgat
     9121 gcggtaaaag atgattttga tgtgtttatc gattttaccc gtccggaagg tacgctgaac
     9181 catctcgctt tttgtcgcca gcatggcaaa gggatggtga tcggcactac ggggtttgac
     9241 gaagccggta aacaggcgat tcgtgacgcc gccgccgata ttgcgattgt ctttgccgcc
     9301 aattttagcg ttggcgttaa cgtcatgctt aagctgctgg agaaagcagc caaagtgatg
     9361 ggtgactaca ccgatatcga aattattgaa gcacatcata gacataaagt tgatgcgccg
     9421 tcaggcaccg cactggcaat gggagaggcg atcgcccacg cccttgataa agatctgaaa
     9481 gattgcgcgg tctacagtcg tgaaggccac accggtgaac gtgtgcctgg caccattggt
     9541 tttgccaccg tgcgtgcagg tgacatcgtt ggtgaacata ccgcgatgtt tgccgatatt
     9601 ggcgagcgtc tggagatcac ccataaggcg tccagccgta tgacatttgc taacggcgcg
     9661 gtaagatcgg ctttgtggtt gagtggtaag gaaaatggtc tttttgatat gcgagatgta
     9721 cttaatctca ataatttgt
//
LOCUS       DAZPBM010000149         9676 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPBM010000149 DAZPBM010000000
VERSION     DAZPBM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.150"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1712
                     /locus_tag="ACHUZQ_003358"
     CDS             <1..1712
                     /locus_tag="ACHUZQ_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR1026973.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1713..3362
                     /locus_tag="ACHUZQ_003359"
     CDS             1713..3362
                     /locus_tag="ACHUZQ_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1026974.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3367..4143
                     /locus_tag="ACHUZQ_003360"
     CDS             3367..4143
                     /locus_tag="ACHUZQ_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1026975.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4217..5401)
                     /gene="atoB"
                     /locus_tag="ACHUZQ_003361"
     CDS             complement(4217..5401)
                     /gene="atoB"
                     /locus_tag="ACHUZQ_003361"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR1026976.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5432..6754)
                     /locus_tag="ACHUZQ_003362"
     CDS             complement(5432..6754)
                     /locus_tag="ACHUZQ_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR1026977.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6751..7401)
                     /gene="atoA"
                     /locus_tag="ACHUZQ_003363"
     CDS             complement(6751..7401)
                     /gene="atoA"
                     /locus_tag="ACHUZQ_003363"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR1026978.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7401..8063)
                     /gene="atoD"
                     /locus_tag="ACHUZQ_003364"
     CDS             complement(7401..8063)
                     /gene="atoD"
                     /locus_tag="ACHUZQ_003364"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR1026979.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8259..9644)
                     /gene="atoC"
                     /locus_tag="ACHUZQ_003365"
     CDS             complement(8259..9644)
                     /gene="atoC"
                     /locus_tag="ACHUZQ_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR1026980.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2485 c         2605 g         2232 t
ORIGIN      
        1 ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa ttatatttcg ctggcgcaga
       61 acattcagca atctggcttg ttaagcgcag aactgcaaca aaatgggcaa gtgcaggaca
      121 gcattagcac aaaactgtct tttgtggata acagctggcc cgttgaacag cagaaaaatg
      181 tcatgctcgg cggtggcgag aacgcgctga tgttgcccga gcaggctagc aatatccggc
      241 tacaaagtag cgaaacgccg caggagattt tccgcaacaa tcttgatgcg ttagtcgatg
      301 gaccatgggg tggcgtgatc aacaccggta gccgtctgat cccgctcagt ctcgcctggc
      361 gttcgcttgc cgatcatcaa agtgccgccg ctaacgacat tcgtcagatg attcaggata
      421 accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg ctttacctgg tggggtgaag
      481 atggcaatgg tgacgccttc cttacggcat gggcatggta cgccgactgg caggccagcc
      541 aggcgctcgg cgtaacgcaa caaccggaat actggcagca tatgctcgac agttatgccg
      601 agcaggcgga taacatgccg ttattgcatc gggcgctggt gctggcgtgg gcacaggaga
      661 tgaatctgcc gtgcaaaacg ttgttgaaag ggttggatga agctatcgcc cggcgcggaa
      721 ctaaagatga agatttctct gaggaagaca tccgcgatat caatgacagc ctgatcctcg
      781 atacaccgga atctccactg gcagatgcgg tggcaaacgt cttaaccatg acgttgctga
      841 aaaaagcgca gttgaagtcc acggtgatgc cacaggttca gcaatatgcg tgggataaag
      901 cggcaaacag caatcagccg ctggcgcaca cggttgtgct gctcaatagc ggtggtgacg
      961 ctacccagac ggccgctatt ttaagtggtt tgaccgctga gcaatccact attgagcgcg
     1021 cgctggccat gaactggctg gcgaaatata tggcgacaat gcctccagtt gttttgcctg
     1081 cgcctgcggg cgcatgggct aaacataagt taactggagg gggcgaagac tggcgttggg
     1141 ttggtcaggg tgtgccggac attctctctt ttggtgacga attatcgccg caaaatgtgc
     1201 aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg gtgaccgttg
     1261 aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact ctgcagccgg
     1321 tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg cttaccagcg
     1381 agcaggatgc agttctgcgc tatggtcagg tggaagtacc gctcccgccg ggagccgacg
     1441 ttgagcgcac aacatggggc atttcagtca ataaacccaa cgccgcgaaa cagcaggggc
     1501 aattgctgga aaaagcgcgt aatgaaatgg gcgaactggc ctatatggtg ccggtgaaag
     1561 aactgacggg aacggtcact ttccgccatt tgctgcgctt ctcgcaaaaa gggcaattcg
     1621 ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc gcagcaaagt gttgcggcag
     1681 ggagtgaatg gaccgggatg caggtgaaat aagtgaactg gcgcagaatc gtctggctat
     1741 tggcgttggt tactctgcca acgcttgcgg aggagacgcc gttacaactg gtgctgcgcg
     1801 gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa gtctcagcat
     1861 tacctgactc gctcaccacg ccgttaggga gtttgtggaa actgtatgtt tacgcctggc
     1921 tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc ccggaagagg
     1981 tttattgttg tcaggctggg gagagcataa cgcgcgatac cgcgctggta cgttcctgtg
     2041 ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg caatactggc
     2101 aacagcgtca ggcacctgcg tggttagcgt ctcttacaac gctgaagcca gaaacctcgg
     2161 taacggttaa atcgctgctc gattcattag cgacgttacc tgcgcaaaac aaagctcagg
     2221 aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatcc atgctcggta
     2281 gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga taaacaggaa atccgccagg
     2341 gtggttttgc tggctggtta accgatggta caccgctgtg ggttactggc agtggcacca
     2401 gcaaaacggt actaacccgt tatgcgacgg tgcttaatcg ggttttgcca gtgccgatgc
     2461 aggttgccag tgggcaatgt gtagaggtgg agctgtttgc ccgctatccg ctgaagaaaa
     2521 tcacagcgga aaagagtacg acggcagtaa aacccggcgt acttaatggg cgctatcgtg
     2581 tgacgtttac taatggtaac cacattacgt ttgtcagcca cggtgagacg accttactga
     2641 gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg tgaagagtac gttgccagag
     2701 ttctggatcg cgaggcgaaa agtacgccac ctgaggcagc gaaagccatg accgtggcga
     2761 tacgtacttt tttacagcaa aacgccaatc gggaaggtga ttgcctgacc atccccgaca
     2821 gcagcgccac gcagcgtgtt tctgcttcgc cagcgaccac gggggcgcga acgatgaccg
     2881 cttggacgca ggatctcatc tatgcaggcg atccggttca ttatcatggc agtcgcgcta
     2941 ccgaagggac actttcctgg cggcaagcca cggcccaggc ggggcagggg gagcgttacg
     3001 accagatact cgcgtttgct tatcccgaca acagccttag ccgctgggga gctccacgct
     3061 caacctgcca gttattaccc aaagcgaaag cctggctggc gaaaaaaatg ccgcagtggc
     3121 ggcgtatatt acaaggcgag acggggtaca acgaaccaga cgtgtttgcg gtctgtcgtt
     3181 tagtctcagg tttcccctat accgatcggc agcagaaacg gctgtttatt cgtaacttct
     3241 ttacgcttca ggatcggctg gatttaaccc atgagtatct gcacctggcc tttgatggtt
     3301 atcccaccgg gcttgatgag aactatatcg aaacgctgac ccgacaatta ttaatggact
     3361 aatgctatgc gaaagatttt tcttccgttg ttactggtgg cactttcgcc tgtcgctcac
     3421 agtgaaggtg tgcaggaagt cgagattgac gcaccgctct ctggctggca tccggcagag
     3481 ggcgaagatg ccagtttttc acaatcgatc aattacccgg cgtcgtccgt caacatggca
     3541 gatgatcaaa atatctcagc gcagatccgc ggcaaaataa agaattatgc tgcggcgggt
     3601 aaagttcagc agggccggct ggtggtcaac ggtgccagta tgccgcagag aattgaatcc
     3661 gatggttcat ttgcacgccc ttatattttc actgaaggca gcaacagcgt gcaggtcatc
     3721 agcccggatg ggcaaagccg gcaaaaaatg cagttttact caacgtcggg tataggaacg
     3781 attcgtgcac gtttacggct ggttctctcg tgggatacgg acaataccga tctcgatctc
     3841 catgttgtca cgcccgatgg cgaacacgcc tggtacggta acaccgtgct gaaaaacagt
     3901 ggtgcactgg atatggatgt cacgacgggg tacggacccg agattttcgc catgccagca
     3961 ccagttcacg ggcgctatca ggtgtatatc aactactatg gtgggcgcag cgaaacggaa
     4021 ttgacgaccg cccaactgac gctgatcacc gatgaaggat cggtcaatga gaaacaggaa
     4081 acatttattg taccgatgcg taatgctggc gagctgacgc tggtgaaaag ttttgactgg
     4141 tgattaatgg ggttaccttc gccgtcgatg ttcaagaaaa cgcccgataa ctttcgctat
     4201 cgggtgtttt tattgattaa ttcaaccgtt caatcaccat cgcaattccc tggccgccac
     4261 caatgcacag tgttgccagc cccagcgttt tatcgcgtgc ctgcatggca tgtaatagtg
     4321 tgaccagaat acgagcacca ctggcaccga taggatgccc gagcgcgatg gccccgccgt
     4381 tgacattcac tttctcagaa tcaaagccca gggttttccc aacggcgagg aactgtgcag
     4441 caaatgcttc attagcttcg atgagatcaa tgtctgccag ttgcagcccc gccagttgta
     4501 acgctttttg cgtggcaggt actggcccca tacccatcaa tgcgggaggc acgccaccgc
     4561 tggcataact tttaatgcga gccagggggg taaggcctgc tgccagcgcc gcagattctt
     4621 ccataatcac cagagcggca gcaccgtcgt taataccaga cgcgttccca gcggtgactg
     4681 ttcctgcttt atcgaaggcc gggcgcaatg cacctaacgc ttcagccgtt gaattcgctt
     4741 tcgggaattc gtcttgactg aagacgaagg ttttcttccg agtgacaaca tttaccggga
     4801 cgatttcggc tgtaaaagca ccggactcaa ttgcggctgc cgctttacgc tgtgaatgta
     4861 gcgccagttc atcctgcatt tcacgggtaa ttccgtactc tttagccacg ttttcggcgg
     4921 taatccccat atgataacca tgggtggcgc acatcaggcc atcgcgcagg attacgtcat
     4981 aaacctgtcc gtctccaaga cgataaccag agcgtgcttt tgcatcgagt aagtagggcg
     5041 ctaaactcat attttccata ccccccgcca caatgctctt cgcctgacct gcctgaattg
     5101 cctgggcggc aagcgccaca cttttaagac ccgaaccaca cactttattg accgtgaatc
     5161 cgcacaccgt ttctgccagc ccgcttttta acagtgcctg acgtgccgga ttttgcccca
     5221 acccggcttg caacacgtta cccataatca cttcatcaat gtgtagtgaa tcgatttttg
     5281 cacgttcaat ggcggcttta attactgtcg cccccaggtc gatggcgctg gtggaagcga
     5341 gtgaaccgtt aaaactaccg atagcagtac gtaccgcact gacgatgaca caatttttca
     5401 ttttatattc cttctgtctg tagggttgcc gtcagaacag cgttaaacca atgacgaaaa
     5461 tgacgccgga gaagagcagg gcagtgatgc aatagcccat gatgtcgcgg acaccgagtc
     5521 cggcgattgc cagtgctggc agcgcccaga atggttgttc catgttcatc cattgctcgc
     5581 cgtaggcgat cgccattacc gatttaccga gatcagcgcc cagcgcctgg gctgcgggta
     5641 tcacgaaagg tccctgaata acccagtgac cgccgccaga cggaacggcg aagttaatca
     5701 gtgcagaact aaaaaaggtc attaccggga aggtgtcttt gttcgcaaca ttgatgaaga
     5761 attcggtaat gagtccaccc agaccagaat gctccatcat cagttggatc ccagcgtaga
     5821 aggggaattg caccagaata ccggcagtac tgcgtgctgc cgcgctgata gcacgcatat
     5881 aagccattgg cgttttatgt agcaacagac ccgcaatcat aaacatcagg ttgacggtat
     5941 tgatggtgat gttgaagcca tgttcgctga agtacatcgc caggtaagcg ataccgagtg
     6001 cgccgatgat caacgtcaga atgcggcttt cttccagtcg ctcggatggt ggggcatctt
     6061 tcggtagctg cttttgaaaa tcagcctctt ccatgagtag ttccggatcg atactcacca
     6121 cgtcagacgg ttttggcatc atcatgcggg tgataaatgg catcaccaca atcaacgtca
     6181 cagtgatgaa aatgttaaaa ccactgaaca gagtatcgcc caccgggatc agcccggcga
     6241 tatgctcaac cgggttgccc ggtgttgcag ccaacagagg cattgatcca gagaagccgc
     6301 caccccaggt gagaaaacca atgtaggcgc aggcaatgag caacggataa tcagaaccgg
     6361 ggactcgccg ggcgacttca cgggcaaaca ttgcgccgac aaccaaacca aatccccagt
     6421 tgatgacaca agcgactgaa ccgaagaaag tgaccagcat gacgccctgt acgggcgtct
     6481 ttgcggcgga ggcggcagta cgcagcaaac ttttcaccgg agcagagctg gcaagggcat
     6541 gaccggtaac gatgataagc gccatctgca taccaaacgc cagcaagttc cagaaaccgt
     6601 caccccacat tttcaccatg ctgatcggcg tttgtggtgt taaccaaagc gcgatcacga
     6661 atgttagcaa tgtcagcaac atggcaaaga tcagtggatc gggaagccat cggctgacaa
     6721 aacgcgtcat aaaacgcgat atgcgaccaa tcataaatca ccccgttgcg tattcagatc
     6781 ggcggcgact tcaaaccgag cttctgtttt ggcacgcacg gtggctaaat cacacccgtc
     6841 ggcaatttcg gtgagccaca ttttgccgtc aataaaacga aagacagcca gttcagtaac
     6901 cagcatatgc accgcatgtt gcgcagtgag tggcatggtg cagctgcgca aaatttttgc
     6961 tgaaccatct ttggcgcaat gttccatggc gatgatcact ttgcgcgacc cggtcaccag
     7021 atccatcgcg ccacccatac cgggcaccat tttcccaggc actacccagt tcgcgaggtt
     7081 tgcttcttcg tctacttgca aaccgccgag cacgcaggca tcaatatgac cgccacggat
     7141 tagcgcaaat gacatggcgc tatcaaacat ggctgcaccg ggtaaaacac cgcacggttg
     7201 cccgccagcg ttcaccagat ctggatgcgc tgtcgtgacc gggcctaaac cgaggaagcc
     7261 gttttccgat tgcagagtga tatgaatacc ctccggtaaa taattggcga ccattgtggg
     7321 taaaccgatc cctaagttaa cgatgtcacc atcacgaagc tcttgcgcca cacggcgcgc
     7381 aatacgttgt ttcgcatcca ttatttgctc tcctgtgaaa cgatgatgtg gtcgataacg
     7441 gcaccagggg tgacaatatg gtcaggttgc agctcgccgg tttcgaccag ttcatctggc
     7501 tctaccagcg tgatatcagc cgcaagggct atcagggggt taaagttgcg ggcgctaagt
     7561 tgataggtca ggttgccaag tgtgtcgcaa cgatgagcgc gaattagcgc caggtcggcg
     7621 cgcagtgggc gttcgagcag ccaggtttta ccgtcgagtg tcagtgtctg tttgccttcc
     7681 tctacgacgg tgccgacacc cgttggggtg agaaaaccac caagtccagc tccaccacag
     7741 cgaatttgct cgattagcgt accttgcggc accagaacga cgtccatctc accagatatc
     7801 atgcgccgac ctgtttccgg gttggtgccg atatgtgaag caatcacttt gcggactcga
     7861 ccattgacga tgagcggacc gatgccggta tcaacaaacg cggtatcatt ggctatcaat
     7921 gtcaggtcgc gaacaccaga ttccagtaat gcttcaacca ggcgggatgg agtgccaatc
     7981 cccataaatc cgcccaccat gatggtcatg ccgtcacgaa agaagccggt ggcgtcttgt
     8041 aatgtcatca attttgtttt cattttttat ccctcttgca tacaccgttg tgggtacagt
     8101 caggcaatag caaggggagt gccagaatga gattattgga tttagttatt taactaattg
     8161 aatatgaagg gataaattgg atggggattt ataaggagaa tgagcggcta tgcagaaaat
     8221 tgcgcactgt gcaaatttct gcatagcaag ttttggtgtt atacatccgc cggatcgata
     8281 ccgtattcct ggagtttata catcaatgca cggcgactga tgcccagcat taaagcagtg
     8341 cgggttcggt ttccttcttg ttgttccagc acttccataa tgatgcgttt ttcgacgcgt
     8401 ttaatttcct cttttaaatt acgctcaccg acagaggctg tttttacctc gccagcatta
     8461 cagactggct gacgaatctg tggcggaaga tcctcagaaa aaatgatcgg gcctgaattc
     8521 atcacgacgg cgcgttcaat aacgttagaa agctctcgaa tatttcccgg ccatgaccag
     8581 gcggtaagca gtgacattgc catcggatcg atgtcgataa tatcgcgctg attctcacta
     8641 ctgaattttt gcaaaaagtg attagctaac agggaaatat cttcccggcg atcgcgcaga
     8701 ggcggcagta ttaaatgaat aacgttaagg cgataaaaga gatcttcacg gaaggtgcct
     8761 tcttttacca ttgcctgcaa gtcacggttg gtggcagcaa tgatgcggat atcaactttt
     8821 atggtctgat ggccgccaat ccgttcaaat tcccgttcct gtagaatgcg tagtaatttg
     8881 gcttgtagta ccagcggcat ttcgccaatt tcgtcgagga gcagagtacc ttcgttggct
     8941 cgttcaaata atccctgacg caaggtttgt gcaccagtaa atgcaccttt ttcatgacca
     9001 aacagttcac tttcgagcaa cgattccggc agcgccgcgc agttgacttt aatgaacggc
     9061 ccctttgccc gccgcgaatt gtagtgaatc gctctggcaa tcaactcttt cccggtgccg
     9121 ctttcaccgc taatcaagac gctggcctga gaaagggcaa ttttggcggt gtctttgcag
     9181 atgtccatca tcgccgggct gttggtgaga atgtgccccc attgccagct ggcgctcagt
     9241 gcctggtgca gatgacggat ctcttttttc attgactgga gttgtaaagc gcgctgaacg
     9301 attaaattca actcatcgag atcaaacggt ttaataacat agtcgaaggc tccgcagcgt
     9361 agcgcttcga cggcggtttc cacttccgca taggccgtca tcagaataac gggtgtccgg
     9421 gtctcatggc tgcgcatctc ctttagtgcc ttgatgccgt ccatctctgg catgcggata
     9481 tccatcaaca ccacatcagg gtgaatatcg gcaaacaggt gtaatgctgt gcgtccgttg
     9541 ttcgcacaat gtgtttcgaa tccttgtagt gcaaaagcgg tgctcagcat acggcgaaca
     9601 ttatcttcat catccacaat aaggatgcga ttaatagcag tcatacagtc tgatttccct
     9661 gcgggttgat cggtaa
//
LOCUS       DAZPBM010000150         9648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPBM010000150 DAZPBM010000000
VERSION     DAZPBM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.151"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHUZQ_003366"
     CDS             complement(<1..670)
                     /gene="ydcF"
                     /locus_tag="ACHUZQ_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1026981.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDS"
     gene            complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHUZQ_003367"
     CDS             complement(942..4844)
                     /gene="hrpA"
                     /locus_tag="ACHUZQ_003367"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1026982.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5045..5650
                     /gene="azoR"
                     /locus_tag="ACHUZQ_003368"
     CDS             5045..5650
                     /gene="azoR"
                     /locus_tag="ACHUZQ_003368"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1026983.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5701..7017)
                     /locus_tag="ACHUZQ_003369"
     CDS             complement(5701..7017)
                     /locus_tag="ACHUZQ_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR1026984.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7007..8764)
                     /locus_tag="ACHUZQ_003370"
     CDS             complement(7007..8764)
                     /locus_tag="ACHUZQ_003370"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1026985.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8780..>9648)
                     /locus_tag="ACHUZQ_003371"
     CDS             complement(8780..>9648)
                     /locus_tag="ACHUZQ_003371"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1026986.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2630 c         2274 g         2296 t
ORIGIN      
        1 cgctatcatc gcgtaaacgc ggcagctcgc cagtgagtag tgagagataa cgctcaaccg
       61 gccataatcc ttgtaactga ttaataaaga ttacactgtc tgcgttattt cctaactgag
      121 gaacgaatcc gggataactt aaccagcgtg gtgcatcggg attgtcccca gtcatacggc
      181 ggaacgtcgc catcgtgcgc cgctgcatgg tggggtcctg aacaacgata gccgtatgaa
      241 ctcgttctac ggcctgattc aatagcgcga tgctaaagcg tgcgttttca ccgcagtttg
      301 ttgactggtc ttcaatccag attttttcat gcggaatgtg ccagaactga tgagcgatat
      361 ccgcctggat ggtcgcttct gctctgccag tggtgcggat agtgttgtag tgcggatgct
      421 gtgcgatggc gctatacaaa aaagttgtcg agtgaccgat accaccacta atcagtaaag
      481 gaatttgctg atcgcgagca atcttacatg ccgcgtcgat agtcggcata accgcattgc
      541 ctgcaaggat cacgcaatcg gcctgatacg gcacctcacc ggagaaatca tcctgcgcca
      601 gccactgtcc gataacattt atggcatcta tagttgccgg cgaaagcgtc ggaaacgggg
      661 taatgttcat ggttcctcct tatcgttccc ttaagaatac tgcgtgagat aatagtgaga
      721 agcggagcac tctgaaagga ggaagtcgta tttccttaac cgccactccg caagcctgac
      781 atatctttta aggaaatgct gtagataaac attattcatc ttgcagcaga gaattgtatt
      841 tttctgaatt cctgaatttt tacccttacc gttttagaat taataaaaaa cccggcgcta
      901 aatgcttacc gggttttttc tttatcaggc aaatagcagg gttaaccgct aatctgctcc
      961 atcgcctgca aaatacgctt atctgaaatc ggataaggcg taccaagttg ttgagcgaag
     1021 taactaacac gcaactcttc tatcatccaa cggatctctt tcacgtcttc atcctcacga
     1081 cgtgcaggcg gcagtttgtt gatccattgc tgccacgcct gctggacgtt ttcgactttc
     1141 agcatctgag cacggtcgcg atgtggatca accgccagtt tttccagccg tttttcaatc
     1201 gcctgcaaat atcgcagcgt gtcgcccagc cgtttgaagc cgttaccagt gacaaaaccg
     1261 cgatatacca gcccgcccat ctgcgcttta atgtcagaaa gccccagcgc catcgtcata
     1321 tccacccgcc ccttcagacg tttgttgata ttgaacactg ccgtaaggat ttgctcgacc
     1381 tgcttcgcaa tatccaccac cgtgtcgttc agttcggcac gcactttttc atgcagcgca
     1441 gcaaagcctt cttccgtcca gaccgggcca ccattggcgt cgatcaattt atccacaccg
     1501 caggagatac aatcgtcgat cagctccagc actttgccat acgggttaaa gtacagcccc
     1561 agcttggctt tgtttggtaa cttctcatgt aaatacttga tcggcgaggg aatattcagc
     1621 agcagtagac ggcgaagacc gttccacatt acctgttttt gctccagcgg attatcaaac
     1681 agtttgatcg ccacactgtc gcgttcatcc actagcgctg gccacgcctt cactttgtag
     1741 ttgccacgct tctgttcgta gctttccggc agctgaccaa aactccagat atgcagtccg
     1801 ctctgctcga taccgtcatc cgccaccgca gacagcgttt cctgcacttt gcctttcagc
     1861 gcatctttca gatcttgtag cgagcgccct tcttttagct tcttgttttt gtcatccacc
     1921 acgcggaagg tgattttcag gtgatcgggc acctgatccc agtgccagtc ttcgcggtca
     1981 acggtaacgc cggtcatccg ccgtaactcg cgctcaaggc tgtcgagcaa cggtaactcc
     2041 agcggtgtga cgcggcctaa aaacgcttcg gcgtagtttg gtgcaggcac aaaattacgg
     2101 cgtaccggtt tcggcaacga tttaatcaga gcaatcacca attcgcggcg cagaccgggg
     2161 atctgccatt caaacccgct ttcctcaacc tggttaagta acggcagcgg aatatgtacg
     2221 gtcacaccgt ccgcgtccgc gcctggctca aactgatagc tcaaacgcag cttgagattg
     2281 ccctgatgcc agaagttcgg gtaatccagc tttctgattt tttccgcccc ttctttgatc
     2341 aacatgctct tttcaaagtt gagcaaatca ggcgtttcgc ggctgacttt tttccaccag
     2401 ctatcgaagt gacgagcgga gattacatcg tggctgatgc gctggtcgta gaactcaaac
     2461 agcgtttcgt catcaaccag aatatcgcgg cgacgtgatt tgtgttccag ctcttccact
     2521 tcggcccgca gtttcaggtt ttcacggaag aatgcgtgac gcgtctgcca gtcaccttcc
     2581 accagagcgt ggcgaataaa gagttcacga cataacgccg aatcgatctg gctgtagttg
     2641 accttgcgcg cagcaacaat cggcaaacca taaacagtga ctttttccgt tgccatcacc
     2701 gcgccctgcg cccgttccca gtgcggttcg ctgtaggtgc gtttaatcaa atgctgagca
     2761 acgggttcta cccattccgg gtcgatacgc gcagcaatgc gcccccacag gcggctggtt
     2821 tctaccagtt ccgccaccat tacccatttc ggcggctttt tgaataaacc agaaccgggg
     2881 aagatggaga aacgcgcgtt acgtgcaccg gtatattcct gtttatcggc atctttcatg
     2941 ccgatatggg aaagcaaacc ggtcagtaag gcgatgtgaa tttcacgata ctccgccggt
     3001 tcgctgttga ccggaatgcc aagttctttc accacctggc gcaactgggt gtagatatcc
     3061 tgccattcgc gcacgcgcag atagttgagg taatcggtac gacacaggcg acggaaggcg
     3121 ttggaagaaa gcgccttttg ctgctcgcca agataattcc acagatttac aaacgcgagg
     3181 aagtcggatt ctttgtcgtg gaagcgacga tgtttttcgt ccgatgcctg ctgtttgtcc
     3241 atcggacgtt cgcgcggatc ctgaatggag agcgcggacg tgataatcat cgcctcacgc
     3301 acgcagccat gtttttgcgc ttccagcacc atacgcgcca gacgtgggtc gacaggcaac
     3361 tgcgagagct ggcgaccgag cggcgtcagt ttataggcgc tggcctgttc atccgtggtg
     3421 atcgcgccca gttcttcgag cagacgcacg ccatcctgga tattgcgttt atccggtgct
     3481 tcgacaaacg ggaacgcagc gatatcgccc agccccagcg cggtcatctg caaaataacc
     3541 gaggccaggt tggtacgcag aatctccgga tcggtaaact ccgggcgcga gaggaaatcg
     3601 tcttcggaat agagacgaat acagatccct tcggacacac gaccacagcg gcctttacgc
     3661 tgattggcag acgcctgcga aatgggttca atcggcaaac gctgcacttt ggtgcgatag
     3721 ctgtagcggc tgatacgcgc tgtaccgggg tcgatgacgt atttaatccc cggtacggtc
     3781 agcgaggttt ccgcgacgtt ggtcgccagc acaatgcgcc gtccgctgtg cgactggaac
     3841 acgcggttct gttcgctgtt cgaaagccgc gcataaagcg gcaagatttc ggtatggcgt
     3901 aagttcagct tgttcagcgc atcggcggta tcgcgaattt cccgctcacc actcataaag
     3961 atcagaatgt cgccagggct ttcctgactc agttcgtcta cggcgtcaaa aatcgcctgc
     4021 aactgatcgc gctcggtgtc atcggcttct tcaacaatcg gtcgatagcg cacttccacc
     4081 ggataggtcc gaccggagac ttcgataatc ggcgcattat taaagtggcg cgaaaagcgt
     4141 tccgggtcga tagtcgcgga agtgataatg atttttaggt caggacgccg cggcagcaac
     4201 tctttcaaat agccgagcag aaaatcgata ttcaggctgc gttcgtgcgc ttcgtcaata
     4261 atgatggtgt cgtactgcat cagcaggcgg tcttgctgga tctccgccag caggataccg
     4321 tcggtcatca gcttgaccat cgtgttatca cttacgtgat cgctgaaacg cactttgtaa
     4381 ccgatgcaac cgcccggctc cgttttcagc tcttccgcaa tacggttcgc caccgttctt
     4441 gccgccaggc gacgcggctg ggtatggccg atcagccctt taatcccgcg ccccagctcc
     4501 atacagattt tcggtaactg agtcgtttta ccagaacccg tttccccggc gacgatcacc
     4561 acctggtgat cacgaatcgc ttcgagaatg tcctgttttt tctgactaac cggtaaattg
     4621 tcaggataag taatttccgg tcgtgccgct tcacgcagca ggactttccc tgccgcctgg
     4681 tcaatctctt tcgccatctc ctggaaaatg gcctgttgtg catcaggatt tttaaccttc
     4741 ttcacgccgt gcagacggcg agaaaaacgc agtctgtcac gcagcatcag cgaatccagc
     4801 cgctgctgca aggccgtaaa ggtcaatttt tgttgttctg tcataacgtt agtgggcagt
     4861 agtctgcctg attaattttc tttgaaatga tagatataga gtaccacatc acggctttct
     4921 atgcgttgtt caataaattc gaacataggc ttcgatttat tgcgctatct ctgtggcagg
     4981 attgtgaata aagtgtcaac aagcaacggg gcatcgccca ttcaaacatc tataaggaaa
     5041 caccatgagc aaggtattag ttcttaaatc cagcatcctg gcagggtact ctcagtctaa
     5101 tcagttgtcc gattattttg ttgaacaatg gcgcgaaaag cactccgctg atgaaatcac
     5161 cgttcgcgac ctggctgcaa atccgattcc ggtactggat ggcgaactgg ttggtgcact
     5221 gcgtccgagc gatgcgccgc tgactccgcg tcagcaggaa gctctggcgc tttccgatga
     5281 gctgattgcc gagctgaaag cccacgacgt tatcgttatt gcggcaccga tgtataactt
     5341 caacatctca actcagttga aaaactattt tgacctggtt gcacgcgcag gcgttacttt
     5401 ccgctatacc gagaacggtc cggaaggtct ggtaacgggt aaaaaagcca tcgttattac
     5461 cagccgcggt gggatccaca aagatggacc aacggacctg gtgacgccgt atctgtccac
     5521 gttcctcggc tttatcggca ttactgatgt gaaatttgtc ttcgccgaag ggatcgcata
     5581 cggtccggaa atggcagcga aagcgcagtc tgacgcgaaa gcagccatcg acagcattgt
     5641 tgctgcataa ccggacacga tcttgatgtg gactgtagtc cagtccacat cccaatccac
     5701 ttacctgttt tcccataatt tcagcatcgc tttgtgatct tccttaagca caatatgcga
     5761 gcgtctggct cgaataaagc taatcgcttc atcaacggtt ttacagtatc cgtaacataa
     5821 cagccatgcc gccactacca gcgcactgcg cgataatccc agtgcgcaat ggaccagaac
     5881 gctgccttgc tcttcgcgta atgtttccag catcgccacg gcctgtcgga gctccccctc
     5941 ttccggaacc accagatcta gcatcggtac acagaaatag agtcgatctt ttgtcgctcg
     6001 tccacgaggg aattcaaagg tgacgtccag aaccgcattc tgtgccggaa tatgtcgtgg
     6061 aaacgcccct aaataaacac cagcagtcat tttgctcacc ggctccaggc gacgacaaaa
     6121 ccaacgcata gatagccaca tcccgatgcg ccagggcaat gtcagccagt aaacggcggg
     6181 cgggagtttc ccctgactat ctttccctgt tgttatcgcg ccaagcccac cgtacccacg
     6241 gccaataatg agtagcgata atactggcca acataaccag actgaccacc ataactgaac
     6301 catcatcatt agctccatca acacaatgca cgcgcacgcg cctacgacat atggcagtgc
     6361 tattttgatt cgacgttgat caggtttctg ataattccac cgacggtcga cgggcaccat
     6421 ccagtcaatc aacataccta ccgccagccc tgtgatgaca tcaataaaat gatgctgcca
     6481 tgtcgtcagc gtcgaaatgg cgatgagtaa aaaccatccg ccgcagactt tacgccacct
     6541 cacagccaga tgctgacgaa agtgacgcca aagtagccag cagagaataa tatgcagcga
     6601 aggagactgg ttataaggca gatcaaacag ttcaagttgc gaaaatagcc atcccgtcac
     6661 cccactcact tcaggacgga taaaactaaa cttcagcgga tagagcaaaa aaccgcagca
     6721 ggccattacc gttgccagaa taagccggtg gacaagtcgg cgctgttcga atgtcgtgct
     6781 acaaacgaac agcgaaaatc catataaaag atccagactc cagtaaggaa caatagtcca
     6841 gggaagaaaa gggatcgccg tttcccaacc gaatacctga ctgggaatat catggctatt
     6901 actgtcctga accgcggtga actgattaag agatccatag gtgaagaaga aaaacggggc
     6961 cagcaacaat aaccagccag ctccttgtag caatacgttc cggcgttcag ttatcacgac
     7021 gcaccgccat cgaaaccgta aaaatgcccc actcatcaat ccgttgtgtg catttatcaa
     7081 atccagcatc atgcacgagc aaatccatct ccccttgcgt acgcacgcgc attacccaca
     7141 gtttaccatc tttatgactg gttaacaccc cggcaatcag ctccagttgt gggtgccacg
     7201 gctgcccggt gtagatgaga atgccacccg gttcgatggc attggcaaga cctgcgagtg
     7261 agttttttac ctgctcattt tcgggaaaca gctcatacag tccagagaca atcgccagcg
     7321 taggccgcgg agttaacgcg ctgagttcct ccgggttaaa cgcatcgccc tgttcaaaac
     7381 gcacccgccc agacattccc cgttgggcaa tcatctcttg cccctgtgca acatttaact
     7441 cactgtaatc acgtaataaa atatcgctta cggcaggctc gtttgccagt gcatccagta
     7501 catagcgccc atgccctgcg gcaatgtcaa ccacgcggac ggctaaacct ttgacgtgga
     7561 gatcggcaac ggcttgttta atcagtattt gtaaatgagt tttgcgctgg cgaataccgc
     7621 gccagccaac actgttcagg tagattttgt cgattaagcg cccgaatgcg ttactgcctt
     7681 gcggttgatt ttgatagaca taatccagcg aactgcctga atcaaagcca gtgctcattc
     7741 cgagatgcag gcccgcagag tgcgtcccga acaatttcat cgctttacgc ataaagcgat
     7801 acgccaaatc aaccggcgat aatggcacgg gtccacctga aaggaggcgc cagcgatccg
     7861 ctgatggtcc agtgcggtct tcatgctgat aatcaaattt ttgtgacttg ttagcatata
     7921 aacgacgaat aaagctttgc attttttcaa atgcctgcgc cctgttctct tcacccaacg
     7981 tgtcgtgata aaagcctggc agcagatgca gctctttcag agggctacgt aatctctgat
     8041 aaaaatcaat ctgcggttgg cgatgcacca catagtcatc gcctgatatc agaagttgcg
     8101 tggggagcgt aatcgccgcc gcatctctaa taatacgttc agacgtttta tagagatcga
     8161 gcaagatatt gacggcaatc gcccgtgtga tcagcggatc attattgaaa ctcgccaccc
     8221 gttgccgatc gtgggtcaaa tagcgtcttt tcacatagga attaataaaa aacagaccac
     8281 gcagacgatg ccatagcgtc agcgcaggac gtgccagcgg cacatacaat ttaaccttaa
     8341 aggccggaga agccagcacc agcccgcgaa ttgcaggcgc ataatcatga acccatgtgg
     8401 caaccagcac tgcgccgacg ctttgcgcga tcacaaccac ctcttccagt ccaacctggc
     8461 tgtcgctggc agcaaaacgg acaaattcat cgacatcctg cactgaacgc gcaagagatg
     8521 ggctataacc acgcggcccc gaagtttttc catgcccccg ggcatcccat gcataaaaag
     8581 cagtatctgg catcgccagt tcatcaacga tatgttgtag acgaccagaa tgttcatggc
     8641 cacgatgaaa taagacgatg acctttttcg cccctggctg taaagcgggc cagtgccgat
     8701 aaaacaacgc tgtattatca ctggtggtaa aaaaatgttc cccagagatg cgtgaatttt
     8761 ccattttctc tattttcctt cagtaacagc agtaacgaat aaaataaaaa aataccggag
     8821 cggtgaaaat taatgagtca atcctgtcaa ggattccgcc atgtccaggt aatagtttcc
     8881 cgctatcttt aacaccaaca tctcgtttga tggctgacat cactacatcg ccgcaaaaac
     8941 cactaatacc aattaacaaa cccgctaata ttgcctgtaa tgtatttagc ggtgtcagta
     9001 acggtccaat gatcatcgac gctatcatgg tggtgattac gccgccgacc agaccttcca
     9061 gcgtttttcc cgggctgact ttggggacca cttttcttct gccgcaggat ttcccccata
     9121 aatactgtgc aatatcgttt gacgcggtta atgccaaaag aaataacacc agtaaggccc
     9181 cgccctggat attcatggat ggcaacatta ataaccaggc ggcatgactc aaggcgaaaa
     9241 cggtcattat ccagccccaa aaaatggcgc tcacggtatg cagaaaaccg gaggggtcac
     9301 ccacaaaaac tcgccaggtg gctaatatca gaaacccagc caaaggtata aataacagaa
     9361 acagctcaaa gcaattaaaa ccgataagca aatagttaag aggaataacc caatataacc
     9421 accgcggaaa atgtacggat ataagcatac aatattcttt caatgccaga aaacttatca
     9481 gcgcaaaaaa cgccaacgtc atccagcgtg gacctgaaat caccagtgaa aaacaaatga
     9541 cgatgcccca ccaggtacga atacgtaatg taacctcacc cccttttctc tccggtaacc
     9601 gccatagcag aaaacggttt atcagcgttg ctaaaattaa caacgcga
//
LOCUS       DAZPBM010000151         9623 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPBM010000151 DAZPBM010000000
VERSION     DAZPBM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.152"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..2201
                     /locus_tag="ACHUZQ_003372"
                     /pseudo
     CDS             1..2201
                     /locus_tag="ACHUZQ_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2194..3330
                     /locus_tag="ACHUZQ_003373"
     CDS             2194..3330
                     /locus_tag="ACHUZQ_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1026987.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3327..5327
                     /locus_tag="ACHUZQ_003374"
     CDS             3327..5327
                     /locus_tag="ACHUZQ_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR1026988.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5452..5913
                     /locus_tag="ACHUZQ_003375"
     CDS             5452..5913
                     /locus_tag="ACHUZQ_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1026989.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5954..6424)
                     /locus_tag="ACHUZQ_003376"
     CDS             complement(5954..6424)
                     /locus_tag="ACHUZQ_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1026990.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6471..7190)
                     /gene="btsR"
                     /locus_tag="ACHUZQ_003377"
                     /gene_synonym="yehT"
     CDS             complement(6471..7190)
                     /gene="btsR"
                     /locus_tag="ACHUZQ_003377"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1026991.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7187..8872)
                     /gene="btsS"
                     /locus_tag="ACHUZQ_003378"
     CDS             complement(7187..8872)
                     /gene="btsS"
                     /locus_tag="ACHUZQ_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1026992.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9094..9159
                     /locus_tag="ACHUZQ_003379"
                     /pseudo
     CDS             9094..9159
                     /locus_tag="ACHUZQ_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9387..>9623
                     /locus_tag="ACHUZQ_003380"
     CDS             9387..>9623
                     /locus_tag="ACHUZQ_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1026993.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2355 a         2532 c         2469 g         2267 t
ORIGIN      
        1 agcggccacg atacgtattg attgaaggcc cggctgattt taatgaccgg gtagacgaac
       61 tgtttttagc ccaccagctt ccggtagcta tttacagtta ttgccagtat caggacggtg
      121 cagcccccgg gcgtggtgcc tggacgccat ttgctgaatt ttcgccggag tggcaggcgc
      181 tacaagccgc acgtcgtatt caggcacaaa cttacttcat cgatttgcct tgctgggcgc
      241 agagtgaaga agtggacgat tcgcctgaca cgcaagagga aagccaggcc ttgctgctgc
      301 gtgccacccg catggataac agcgacaccc tgtgggatca cttgttcgaa gatgaaagcc
      361 agcaaactgc attaccctct gcactggcgc actattttgc ccaactgcgg ggcgattccc
      421 ccggcgatgc actcaatcgt cagcgcgaag cctttatggc ccgctggatt ggatgggcga
      481 tgcagcaaaa caatggcgac gtgttagtcg tctgcggtgg ctggcacgct cctgcactgg
      541 caaaaatgtg acgcgaatgc ccgcaggaca ttaacacgcc ggaattgccc tcgctggcag
      601 atgccgttac aggttgttat ctcaccccct acagtgaaaa gcgccttgat gtgctggcag
      661 gatacctttc cggtatgcct gccccggtct ggcaaagctg gtactggcag tggggcttac
      721 agcaggcggg agaacaactg ctaaaaacgg ttcttacccg tttgcgccag cacaaattgc
      781 ctgcttcgac cgcggatatg gctgccgctc atctgcatgc gatggcgctg gcacagttgc
      841 gcggacataa actaccgtta cgcactgact ggctggatgc catagcaggc tcgctgatta
      901 aagaagccct gaacgcgccg ttgccgtgga gctatcgcgg agttattcat cccgacaccg
      961 atccgattct gctaacgttg atagacacat tagcgggtga cggattcggt aaacttgccc
     1021 cttctacgcc acaaccgcct ctgccaaaag atgtcacctg cgaactggaa cgtaccgcaa
     1081 tctctcttcc ggcggagctt accttaaatc gctttaaccc caatgggcta gcgcaaagtc
     1141 aggtgttaca tcggctggca atactggaga tcccagggat tgtacgccag cagggaagca
     1201 cactgacact tgcaggcaac ggtgaagaac gctggaaatt aacccgcccg cttagccagc
     1261 atgcggcatt gattgaggcc gcctgctttg gtgccacact ccaggaagcc gcacgccata
     1321 aattagaagc cgatatgctg gacgcgggtg gaatcggcag tatcaccaca tgtcttagcc
     1381 aggcggcgtt agcgggtctg gcgtccttca gtcaacaatt actggagcaa ctcacattat
     1441 taatcgccca ggaaaatcaa tttgccgaaa tgggccaggc gctggaagtg ctatatgcct
     1501 tatggcggct ggatgaaatt agcggtatgc aaggcgcgca gatattacaa acgacgttat
     1561 gcgcggctat cgatcgcacg ctgtggctgt gtgaatctaa cggcagacca gatgaaaagg
     1621 agtttcacgc tcacctgcat agctggcagg cgctttgcca tatcctgcgc gatctacata
     1681 gcggcgttaa tttacccggc gtttttcttt ctgcggcggt agccttactg gagcgccgca
     1741 gtcaggcgat tcatgtcccg gcgctggatc gcggcgcggc tcttggcgca ctgatgcgtc
     1801 tggaacatcc caacgccagt gccgaagcgg cgctgacgat gctggcgcag ttatccccgg
     1861 cacaatccgg cgaggcgctg cacggtttgc tggcgctcgc ccgtcatcaa ctggcctgcc
     1921 aaccagcatt tatcgccggt ttcagtagtc atttaaatca actgagtgac gacgatttta
     1981 ttaacgccct gcccgatttg cgcgcggcga tggcctggct accgccacga gaacgcggga
     2041 cactggcgca tcaggtgctt gagcattacc agcttgcgca gcttcctgtt tcagcgctgc
     2101 aaatgccgtt gcattgtcct ccgcaagcca ttgcacatca tcaacaactc gaacagcagg
     2161 cactggcatc gctgcaaaac tggggagttt tccatgtctg aactgaacga tcttctgacc
     2221 acccgtgagc tacaacgctg gcgattaatt cttggcgaag cggcagaaac gacgctttgt
     2281 gggctggatg acaacgcccg gcagatagac cacgcgctgg aatggctgta tgggcgcgat
     2341 cctgaacggc tccagcgtgg tgaacgctcc ggtggattag gtggctcaaa tctcaccact
     2401 cctgagtgga tcaacagtat tcacacgctg tttccgcaac aggtgattga gcggctggaa
     2461 agcgatgccg tactgcgcta cggcattgaa gatgtggtga cgaatctcga catgctggaa
     2521 cgtatgcagc cttctgaaag cctgctacgc gccgttttgc acaccaaaca tctgatgaat
     2581 cccgaagtac tggctgccgc ccgccggata gtgcgccagg ttgttgaaga aattatggct
     2641 cgactggcaa aggaagttcg tcaggctttt tctggtgtcc gcgatcgccg tcgccgctca
     2701 tttattccac tggcgcgaaa ctttgatttc aaaagtactc tgcgcgctaa cctgcaacac
     2761 tggcacccgc aacacggcaa gttgtatatc gaatcccccc gctttaacag ccgcattaaa
     2821 cgccaaagcg aacaatggca actggtctta ctggttgatc aaagcggatc gatggtggac
     2881 tcggtgatcc actctgcggt gatggcggcc tgtttgtggc agttacccgg cattcgtacc
     2941 catctggtgg cgtttgacac aagcgtggtt gatctcacgg cagacgttgc cgatccggta
     3001 gagttattaa tgaaagtaca gttgggcggc gggaccaata tcgccagtgc cgtggagtat
     3061 ggtcggcaac ttattgaaca accagcaaaa agcgtcatta tcctcgtgag cgatttttac
     3121 gaagggggtt catcatcatt gctgacgcat caggtgaaaa agtgtgtcca gagcggcatc
     3181 aaagtgctgg gactggctgc gctcgatagc accgcaacac cttgctatga ccgcgatacg
     3241 gcccaggcgc tggttaatgt cggcgcacaa atagccgcca tgacgccggg cgagctggca
     3301 tcatggcttg cggagaatct tcagtcatga attcactacg tccggaatta ttagaactga
     3361 caccgcaggc cctgacggcg ttaagcaatg ccggttttgt taagcgcagt cttaaggaac
     3421 tggaaaatgg caacgtcccg gagatcagcc atgagaacga cgctttaatc gccaccttca
     3481 gtgacggtgt ccgtacccag ctggcgaacg gccaggcact gaaagaggct cagtgcagtt
     3541 gcggggccaa cggtatgtgc cgtcatcgcg tgatgctggt gttaagttat caacgacttt
     3601 gtgccaccac tcagtctacg gaaaaagaag aagagtggga tccggcaatc tggctggaag
     3661 aactggctac ccttcccgat gctacccgca aacgcgcaca ggcgctggtc gctaaaggca
     3721 tcaccattga gttgttctgt gcgccgggtg aaattccctc tgcccgctta ccgatgagcg
     3781 atatgcgttt ttattcccgc agcagtattc gtttcgcccg ttgtgattgt attgaaagca
     3841 cactttgcga acatgtcgta ctggcagtac aggcctttgt ccaggcaaaa gcgcagcaag
     3901 cggaattgac tcatttaatc tggcagatgc gcagcgaaca cgtcacatca tctgacgatc
     3961 cgtttgccag cgaagaaggc aaaacgtgtc gtcaatatgt tcaacaacta agccaggcat
     4021 tatggctgag cggcatcagc cagccgctta ttcactacga ggccgctttc agccgcgcgc
     4081 agcaagcagc ggaacgctgc agctggcgat gggtgagtga atcgctacgg caactgcgcg
     4141 ccagcgttga tgccttccac gcccgcgcca gccactatca tgccggagaa tgcttacgtc
     4201 agcttgcggc attaaacagt cgattaaatt gcgcacaaga gatggcccgg cgcgacagta
     4261 ttggtgaagt tcctcctgtg ccgtggcgca cggtcgttgg ctctggcatt gccggagaag
     4321 caaagcttga tcatctgcgg ctggtgtctt taggtatgcg ttgctggcag gatattgagc
     4381 attatggttt acgcatctgg tttaccgatc ccgacaccgg cagtattttg cacctttcgc
     4441 gcagttggcc gcgaagtgaa caggaaaact caccggcagc tacgcgtcgg ctgtttagtt
     4501 ttcaggctgg cgcactagcg ggcgggcaaa ttgtttcaca agcagcaaaa cgcagtgccg
     4561 atggcgagct gctgttagct acccgcaacc gcttaagcag cgttgtgccg ctgtcgcctg
     4621 atgcctggca aatgttgagc gcgccgttac gccagccggg cattgtggct ttgcgggaat
     4681 atttacgcca gcgtcccccc gcctgcatac ggcctcttaa tcaggtcgat aacttattta
     4741 ttctgccggt cgctgagtgt atttcgctcg gttgggatag cagccgccag acgctggatg
     4801 cgcaggtaat cagcggcgaa ggtgaagata atctcctgac gttatccctt ccggcatctg
     4861 cctgttctcc ttttgccgtt gaacgcatgg cggcgctttt gcaacaaaca gacgaccccg
     4921 tgagtctggt ttctggcttt gtcagctttg tagaggggca attgacactg gaaccacggg
     4981 tgatgatgac aaaaacccgc gcctgggcgc tggacgcaga aactgcgcct gtggcaccgc
     5041 taccttctgc cagcgttttg cctgtgccgt ctaccgctca tcagttgctg atgcgctgcc
     5101 aggcgttact tattcaactg ctccataacg gctggcgcta tcaggaacag agcgctatta
     5161 gtcaggcaga gttgctggcg aatgacctct ccgcggtcgg tttttatcgg ctggcacatg
     5221 tgttgggaca atttcgtaat acagaaagcg aggcacgggt agaagcaatg aataacggtg
     5281 ttttactttg cgaacaatta ttccccatgc ttcagcaaca aggatgaaat agtgcttttt
     5341 actaagagtt ctactccagt tccggactgc tcacgccacg gtattaggca tatcctatat
     5401 agcccctggt gagagtcacc agttccttga ttaaataaaa tggagtttta catgaaggct
     5461 ttcaataagc tgttttccct cgttgttgca tctgttctgg ttttctctct tgctggctgc
     5521 ggtgacaaag aagaatcgaa gaaattcagc gccaatctga acggcactga aattgccatt
     5581 acctatgtct acaaaggtga caaggtgctt aagcaatctt ctgaaaccaa aattcaattt
     5641 gcctccattg gtgcaaccac caaagaagat gctgccaaga cacttgagcc gttaagcgcc
     5701 aaatacaaaa acatcgcggg tgttgaagaa aaattaacct ataccgatac ctacgcgcag
     5761 gaaaacgtga ctatcgatat ggaaaaagtg gattttaaag ccctgcaggg tatttcagga
     5821 atcaacgttt ctgctgaaga tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg
     5881 atgaaagccg ctggttttaa agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc
     5941 cgccataccc gtcttagctt ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat
     6001 aacgtaaaaa ttgatcgccg cattcgcgga agtttttatg atccggtgcg cgcatcatcg
     6061 ctgtaatttc cggcatcgaa acgcggaact gctgttcggt gaggatcgcc agaatgtcat
     6121 cggttttcag cgaaaacgcg atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac
     6181 gttccggctc cagtgccgga gcagactcat ccttgccgcg tttttcataa atcaggccat
     6241 tgaggaatga cgacaaaaca atgtccggac aacgctgaaa accctcttcg tcttctttac
     6301 gtagccagac ggcgatctgt tccgcggtgg cttcgacatt acccagcgcc agaatacgca
     6361 ccaggtcatt attattggct ttcaaaatgt agcgcacgct gcgcagaata tcgttactta
     6421 gcatgaggcc ttcaggtgtt gatgaggcaa aaagccattt tagcagtctt ttacaggcca
     6481 atcgcctctt ttaagctttt cagataacgg cggctgaccg gcacggttaa gccattacgc
     6541 aaaatcaact cggcctggcc gttatcttcc agacgaatct cctgtaaatg cgcgaggtta
     6601 accagatact gacgatggca gcgcagtagt ggtgtacgac tttccagggt acgtaatgtc
     6661 aattcggtaa agccctcttt cccttcgtgg ctggtaacgt agacaccgct catccgactg
     6721 ctgacaaatg ccacatcttt catttgcagc aaataaatcc gactatgccc cgtacaaggg
     6781 ataaatttca gcgcctgttg attttccggt aacagcgaaa catcctgctt gctgcgctcc
     6841 tgacgcaatc gcgccagcgt tttctccagt cgcgcttcat caattggctt cagcagataa
     6901 tcaaaggcat gttcttcaaa ggctttaata gcgtattcgt caaacgcagt gagaaaaaca
     6961 atatacgggc gatgttccgg gtcaagcatc cccaccattt ccagaccact gatgcgcggc
     7021 atctggatat cgagaaacag cacatccggg cgcagtttat gcaccgcgcc gatcccttcc
     7081 acggcgtttg aacactctcc aacgatttca atatcgctct gctcctgcaa aaatacacgc
     7141 aggttctccc gtgctaacgg ttcatcatcg acaattaaga ctttaatcat gcctcgtccc
     7201 tccatggtag tcgtaacgtt attcgggtgt aactatcagg ctcacaggcg acgcttattc
     7261 catagtcatc gccaaaccgt tcacgtaaac gcttatccac cagattcatc cccagcccac
     7321 tggcattggt taccggttga tacaaaccgg cattgtcttc gatctccagc atcaaatgtt
     7381 gcccctcacg tcgggcgctg attgccactc gccctgtatc cagcagttgt gatgtcccat
     7441 gtttaatggc gttttccact atcggttgca gggtaaacgc gggcaattgc tgctgggata
     7501 attcttgcgg aatagcaatg ttgacctgca accgcgactg gaagcgcgcc ttttcaattt
     7561 gcagataagc gttcacatgt tcaatttcgt cggcgagagt aacaaactcc gaaggccgct
     7621 ttaagttttt gcggaaaaaa gtggaaagat actgcaccag ctggctggcc tgttcgctgt
     7681 cgcggcggat caccgcttta atggtgttaa gcgcattaaa caaaaaatga ggattcacct
     7741 gggcgtgaag cagtttgatt tctgactggg tgagcatcgc tttttgccgc tcatattgcc
     7801 cggcaagaat ctgcgccgaa agcagttgcg caatcccctc gcccagcgtg cggtttattg
     7861 aactgaataa acggtttttg gcttcataca atttgatggt gcccatcacg cgctgatttt
     7921 cgccacgcaa cggaattacc agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac
     7981 gataaggtac ttcgttgcca tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg
     8041 tgtaaggcga agaaatcggt ttgccaggta aatggtggtc gtcaccaatt ccggtaaagg
     8101 ccagcaattt ctctcgatcg gtaatcgcga ctgcaccaat atccagctcc tgatacagca
     8161 cctgagccac tttcatgctg ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg
     8221 aggctgccac tttcagcgca gtggcagaaa aagccgaagt gtatttttca aacatcgcgc
     8281 gtttatcgag caatatacgc ataaacagcg ccgcgccgac ggtattggtg accatcattg
     8341 gcgcagcaat attactcacc agacgcaccg catcttcata aggtcgggcg atcgccagga
     8401 tgatcagcat ttgcaccatt tcagcgacga acgtgacggc accggcggta atggggttaa
     8461 agactttatc agtgcgcccg cgacggatca ggatgctgtg taccaggccg ccgagcaatc
     8521 cttcaacgat ggttgagatc atgcaactta gcgcggtcat gccccccatc gaatatcgat
     8581 gtaagccgcc ggtcagacca accagcccac cgacgaccgg accgccgagt aagccgccca
     8641 ttaccgcgcc tatcgcacgg gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg
     8701 tgcccatgat gcagaagatg gaaaagacga tgtagcagag aaatttatgc ggcagacgaa
     8761 ccgtgacctg cattaacggt atgaataatg gcgttttact cattaaccac gcaatgacta
     8821 aaaaaacgca catctgctga agcagcagca acaccagatt aaaatcgtac atacccgcaa
     8881 accacacttc cctttaaaac gcgtaacata cattgcctgc gtttaacttt ctttgaactc
     8941 ttgcagaaaa atgagaattc gtgagtacga tcactcaaaa tcgcctggca aaaataaaat
     9001 caccctatag atgcacaaaa aacgggcaaa actacctggt tcgcaaaact gcgtctaaag
     9061 ttaaaccggg acctcgcgag caagggtgag acgatggcgc tttacacaat tggtgaagtg
     9121 gcgttgcttt gtgatattaa ccctgtcacg ttacgcgcgt gacgcaacat ttttaaaaat
     9181 cgacgaagat cgtccaggag cgccgttttt caagggttgg atagatgatt cgtttaccat
     9241 gtcgacgaac ttcgacgaat ttcgccgata aaaagtgtcc catacatgcc ccattgatgc
     9301 cccataaaca atatcaggaa ttgacactgg ttatccatac agataaaaat aacacctgta
     9361 cataaccaca gttacaggag ggtgttatgc gtgttgagat ctgtatcgct aaagaaaaaa
     9421 tcactaaaat gccaaacggt gctgtggatg cgttaaagga agaattaacc cgacgcatca
     9481 gtaaacgtta tgacgatgta gaggtgatcg taaaagccac cagcaacgat ggcctttctg
     9541 ttacacgcac cgcagataag gattctgcaa aaacatttgt tcaggagact ctgaaagata
     9601 cctgggagtc tgctgacgag tgg
//
LOCUS       DAZPBM010000152         9500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPBM010000152 DAZPBM010000000
VERSION     DAZPBM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.153"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHUZQ_003381"
     CDS             <1..108
                     /locus_tag="ACHUZQ_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1026994.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHUZQ_003382"
     CDS             complement(149..1231)
                     /locus_tag="ACHUZQ_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR1026995.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHUZQ_003383"
     CDS             complement(1228..3060)
                     /locus_tag="ACHUZQ_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1026996.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHUZQ_003384"
     CDS             complement(3275..3631)
                     /locus_tag="ACHUZQ_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1026997.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHUZQ_003385"
     CDS             complement(3689..4111)
                     /locus_tag="ACHUZQ_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1026998.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHUZQ_003386"
     CDS             complement(4127..4852)
                     /locus_tag="ACHUZQ_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR1026999.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHUZQ_003387"
     CDS             complement(4874..5620)
                     /locus_tag="ACHUZQ_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1027000.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHUZQ_003388"
     CDS             complement(5627..6691)
                     /locus_tag="ACHUZQ_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR1027001.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHUZQ_003389"
     CDS             complement(6763..7188)
                     /locus_tag="ACHUZQ_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1027002.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHUZQ_003390"
     CDS             complement(7185..7448)
                     /locus_tag="ACHUZQ_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1027003.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHUZQ_003391"
     CDS             7560..8060
                     /locus_tag="ACHUZQ_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1027004.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHUZQ_003392"
     CDS             8080..8274
                     /locus_tag="ACHUZQ_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR1027005.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHUZQ_003393"
     CDS             8274..8564
                     /locus_tag="ACHUZQ_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1027006.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8843..8995
                     /locus_tag="ACHUZQ_003394"
     CDS             8843..8995
                     /locus_tag="ACHUZQ_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1027007.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9007..9372
                     /locus_tag="ACHUZQ_003395"
     CDS             9007..9372
                     /locus_tag="ACHUZQ_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027008.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2442 a         2491 c         2017 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaata atgatgctat tgtttcaggc gtcaacccac cccgccccac agaatgcagg
     8701 gcaatacttc gagttaccag acagtggtca ggggttaagt agccagcccg aggcgtaaga
     8761 acatgacggc agggttcaat ctttaactat gcagcaggtt ttttgttccg ccaccccggc
     8821 gttaagggga aatgaggtca gcatggatac tatcgatctt ggcaacaacg aatctctggt
     8881 gtgcggcgtg ttccctaacc aggatggaac gtttaccgcc atgacgtata ccaaaagcaa
     8941 aacgtttaaa accgaatctg gcgcgcgttg ctggttagcc agaaacactg actgatgagg
     9001 ctgacgatgg aatttaaaga tttaccgtct gatgtacaga aaacagcagc tcatacattg
     9061 cattctgtgc tgcgagaaat cgggaaagat attgcaagcg agccagcaaa agatctggcc
     9121 cggaaaatca agaccgcttt cgttgagctt tataatgttg gcactgactc tgaaactgtc
     9181 gagaccaaga ccgtaagttc accaatattc tcacttgagc caagcgtatt atcaggtgaa
     9241 atatgcaccg agatttccag cgaactgctt ccggtaatac gagaagcaat tcgccgtcgc
     9301 ggtttggatg gaagttatga tcatgacgtt ctgcaagttc tcaggacaat ggtgacttca
     9361 ctggggattt gatccctgcg tcctctaagc ttttgatata gcggtcctcc aagaagcggt
     9421 aaattttgtc gaaatcatct tttccctctg gtttattcag aggattacag gacccgtaag
     9481 tagcggcata catttcaacc
//
LOCUS       DAZPBM010000153         9498 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPBM010000153 DAZPBM010000000
VERSION     DAZPBM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.154"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUZQ_003396"
     CDS             <1..173
                     /locus_tag="ACHUZQ_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1027009.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHUZQ_003397"
     CDS             complement(345..674)
                     /locus_tag="ACHUZQ_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1027010.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHUZQ_003398"
     CDS             complement(679..864)
                     /locus_tag="ACHUZQ_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1027011.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHUZQ_003399"
     CDS             complement(861..3500)
                     /locus_tag="ACHUZQ_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1027012.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHUZQ_003400"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHUZQ_003400"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1027013.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHUZQ_003401"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHUZQ_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1027014.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHUZQ_003402"
     CDS             complement(5227..5493)
                     /locus_tag="ACHUZQ_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1027015.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVALGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHUZQ_003403"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHUZQ_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1027016.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2372 a         2470 c         2313 g         2343 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctag
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9481 tattatcttc gatacatt
//
LOCUS       DAZPBM010000154         9496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPBM010000154 DAZPBM010000000
VERSION     DAZPBM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.155"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHUZQ_003404"
     CDS             122..952
                     /locus_tag="ACHUZQ_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR1027017.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUZQ_003405"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUZQ_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1027018.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHUZQ_003406"
     CDS             complement(2081..3541)
                     /locus_tag="ACHUZQ_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1027019.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUZQ_003407"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUZQ_003407"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1027020.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHUZQ_003408"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHUZQ_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1027021.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHUZQ_003409"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHUZQ_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1027022.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHUZQ_003410"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHUZQ_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR1027023.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHUZQ_003411"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHUZQ_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1027024.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHUZQ_003412"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHUZQ_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1027025.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2259 a         2484 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZPBM010000155         9402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPBM010000155 DAZPBM010000000
VERSION     DAZPBM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.156"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="ACHUZQ_003413"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="ACHUZQ_003413"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1027026.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHUZQ_003414"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHUZQ_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1027027.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="ACHUZQ_003415"
     CDS             1394..1915
                     /locus_tag="ACHUZQ_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1027028.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHUZQ_003416"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHUZQ_003416"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1027029.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHUZQ_003417"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHUZQ_003417"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1027030.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHUZQ_003418"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHUZQ_003418"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1027031.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="ACHUZQ_003419"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="ACHUZQ_003419"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1027032.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="ACHUZQ_003420"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="ACHUZQ_003420"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1027033.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="ACHUZQ_003421"
     CDS             complement(6557..7450)
                     /locus_tag="ACHUZQ_003421"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1027034.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="ACHUZQ_003422"
     CDS             complement(7447..8100)
                     /locus_tag="ACHUZQ_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1027035.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="ACHUZQ_003423"
     CDS             complement(8111..9370)
                     /locus_tag="ACHUZQ_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1027036.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2091 a         2667 c         2493 g         2151 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgctg
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaaaccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa ac
//
LOCUS       DAZPBM010000156         9293 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPBM010000156 DAZPBM010000000
VERSION     DAZPBM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.157"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            89..229
                     /locus_tag="ACHUZQ_003424"
     CDS             89..229
                     /locus_tag="ACHUZQ_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1027037.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            232..399
                     /gene="yciX"
                     /locus_tag="ACHUZQ_003425"
     CDS             232..399
                     /gene="yciX"
                     /locus_tag="ACHUZQ_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1027038.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            513..608
                     /gene="ymiC"
                     /locus_tag="ACHUZQ_003426"
     CDS             513..608
                     /gene="ymiC"
                     /locus_tag="ACHUZQ_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1027039.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            772..3447
                     /gene="acnA"
                     /locus_tag="ACHUZQ_003427"
     CDS             772..3447
                     /gene="acnA"
                     /locus_tag="ACHUZQ_003427"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1027040.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3511..4101)
                     /gene="ribA"
                     /locus_tag="ACHUZQ_003428"
     CDS             complement(3511..4101)
                     /gene="ribA"
                     /locus_tag="ACHUZQ_003428"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1027041.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            4271..5035
                     /gene="pgpB"
                     /locus_tag="ACHUZQ_003429"
     CDS             4271..5035
                     /gene="pgpB"
                     /locus_tag="ACHUZQ_003429"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1027042.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5184..5492
                     /gene="lapA"
                     /locus_tag="ACHUZQ_003430"
     CDS             5184..5492
                     /gene="lapA"
                     /locus_tag="ACHUZQ_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1027043.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5499..6668
                     /gene="lapB"
                     /locus_tag="ACHUZQ_003431"
     CDS             5499..6668
                     /gene="lapB"
                     /locus_tag="ACHUZQ_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1027044.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6861..7598
                     /gene="pyrF"
                     /locus_tag="ACHUZQ_003432"
     CDS             6861..7598
                     /gene="pyrF"
                     /locus_tag="ACHUZQ_003432"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1027045.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7598..7924
                     /gene="yciH"
                     /locus_tag="ACHUZQ_003433"
     CDS             7598..7924
                     /gene="yciH"
                     /locus_tag="ACHUZQ_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1027046.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8050..8268)
                     /gene="osmB"
                     /locus_tag="ACHUZQ_003434"
     CDS             complement(8050..8268)
                     /gene="osmB"
                     /locus_tag="ACHUZQ_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1027047.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8537..9286)
                     /locus_tag="ACHUZQ_003435"
     CDS             complement(8537..9286)
                     /locus_tag="ACHUZQ_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1027048.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         2303 a         2177 c         2435 g         2378 t
ORIGIN      
        1 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
       61 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
      121 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
      181 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
      241 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
      301 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
      361 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
      421 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
      481 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
      541 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
      601 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
      661 ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
      721 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
      781 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
      841 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
      901 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
      961 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     1021 gcaagggtgt tgatgcagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     1081 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     1141 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     1201 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga
     1261 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     1321 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     1381 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttggcgtg
     1441 ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca gccggtttcc
     1501 atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga aggtattacc
     1561 gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt ggtggagaaa
     1621 ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg cgccaccatt
     1681 gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga tgctgtaacc
     1741 ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat
     1801 gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac cagtacgtta
     1861 gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgccc acaggatcgc
     1921 gttgcactgc ccgatgtacc aaaagtattt gccgccagta acgaactgga agtgaatgcc
     1981 acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta tcagttacct
     2041 gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa cccaagtgtg
     2101 ctgatggctg caggcttgct ggcgaaaaaa gccgtaattc ttggcctcaa gcggcaaccg
     2161 tgggtcaaag cgtcgctggc accaggctcg aaagttgttt ctgactatct ggcaaaagcg
     2221 aaactgacac cgtttctcga cgaactgggg tttaaccttg tgggatacgg ttgtactacc
     2281 tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat
     2341 ttaaccgtcg gtgcagtgct gtccggcaac cgtaactttg aaggccgtat ccatccgctg
     2401 gttaaaacca actggctggc ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat
     2461 atgaatatca acctggcttc tgagcctatt ggtcatgatc gtaaaggcga tccggtttat
     2521 ctgaaagata tctggccatc ggcacaagaa attgcccgtg cggtagaaca agtctccaca
     2581 gaaatgttcc gcaaagagta cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt
     2641 aacgtcacac gatccgatac ctacggttgg caggaggact caacctatat tcgcttatcg
     2701 cctttctttg atgaaatgca ggcaacacca gcaccagtgg aagatattca cggtgcgcgg
     2761 atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc gggcagtatt
     2821 aagcccgaca gcccagcggg tcgatatcta caaggtcggg gtgttgagcg aaaagacttt
     2881 aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac cttcgccaat
     2941 attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg gcatttacct
     3001 gacagcgacg tagtctctat ttatgatgct gcgatgcgct ataagcagga acaaacgccg
     3061 ctggcggtga ttgccgggaa agagtatggt tcaggctcaa gccgtgactg ggcggcaaaa
     3121 ggtcctcgtc tgcttggaat tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt
     3181 tcgaatttaa ttggcatggg catcctgccg ctggaatttc cgcaaggcgt aacgcgtaaa
     3241 acgttaggac taaccgggga agagaagatt gatattggcg atctgcaaaa tctacaaccc
     3301 ggtgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaggt cgtaccctgc
     3361 cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg cattttgcat
     3421 tatgtcattc gtaatatgct gaagtaacaa atatttgctt gccggttatt ttgcttccgg
     3481 caagcaaatt aattccacaa tgcgagaggg ttatttgttc agcaaatgac ccattttctc
     3541 ggctttggta ttgagataat gttcgttatt ggggttacga cctacaatca atggtacgcg
     3601 ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt tattggttaa
     3661 caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag tgaagtcgcg
     3721 ctcatcagcg gcgaagccta actgatggtt agcctctacg gtatcgtaac cttgatcctg
     3781 cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct gacggtgata
     3841 cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc
     3901 acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat ggacgcgcgc
     3961 aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat cgtgtccggt
     4021 tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca gtttggcttc
     4081 tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta ttggcttatt
     4141 ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac ggaatggcac
     4201 aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga cttatcaaaa
     4261 aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac tattgcttgt
     4321 catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac aaagttggct
     4381 actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg tcattacaca
     4441 tttgatttta ttcggctggt ttctctggtg tctgcgtttt cgcattaagg ctgcctttgt
     4501 attatttgcc attctggcgg ccgtaatcat tgtgggacaa ggcgttaaat cctggatcaa
     4561 agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac atcatattcc
     4621 ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga aagaacagtt
     4681 ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag agacggggtt
     4741 tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg ccgttggttt
     4801 gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctggtctggg caacgggagt
     4861 catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag tagctacgtt
     4921 gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt gtgggccatt
     4981 aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg
     5041 gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc catttcgcag
     5101 acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa tttattcgct
     5161 ttaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg gtgttagcga
     5221 tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg tttaattatc
     5281 tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt gctgcggggg
     5341 ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc
     5401 gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact gacgtggctg
     5461 tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg ttgtttctgc
     5521 ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg caacaaaaca
     5581 agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac ttcctgctta
     5641 gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag gatacaggca
     5701 ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg
     5761 ctattcgcat ccatcagacc ctaatggaaa gcgcctcact gacctatgaa cagcgtctgt
     5821 tggcgattca acaactgggg cgtgattaca tggccgccgg gttatacgac cgcgcggaag
     5881 acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgcgctg caacagttgc
     5941 tacaaatcta ccaggctacc agcgagtggc agaaagcaat tgatgttgcc gaacgcctgg
     6001 tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt gagttagccc
     6061 tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg
     6121 cggcagataa aaacagcgcc cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag
     6181 gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac agagaactgg
     6241 tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa actgccgaat
     6301 gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct gaattgatgc
     6361 ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat attacgcgcc
     6421 agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac cacttaaatg
     6481 aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa
     6541 aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca tacaccctct
     6601 actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc ggtcttgatg
     6661 gcctgtaatt tttaaaaaaa tccgacttta gttacaacat actagttatt gatgttccat
     6721 tgtgctccgg caacgacgga gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat
     6781 tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc
     6841 atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga
     6901 attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg tcctttgtcg
     6961 acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg
     7021 ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt gacctgaaat
     7081 tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
     7141 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac
     7201 tggttccgtt tggcaaagat gcaccgcttt tgattgctgt gacagtgttg accagcatgg
     7261 aagccagcga cctggtcgat cttggcatga cactgtcacc tgcagattat gcagaacgtc
     7321 tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg
     7381 tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacgccgggc attcgtccgc
     7441 aggggagtga agctggtgac cagcgccgca ttatgacgcc agaacaggcg ttgtcggctg
     7501 gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcagacgc
     7561 tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct
     7621 ggtctactca acggaaaccg gacgtattga tgagcccaaa gcggcccctg tacgccctaa
     7681 aggcgacggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg
     7741 cctgattacc ggtgtcgatc tcgatgatgc tgaactgaca aaacttgcag cggaactgaa
     7801 gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa
     7861 gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac tcgcaggcgg
     7921 ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt gcgtattatt
     7981 cagttatttc gtactgaaca gaatcaagcg tattataatt gcgagtcgat aatcgacatt
     8041 tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg caccacctaa
     8101 tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc
     8161 gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccaa
     8221 agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc
     8281 tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga ataagcctgg
     8341 tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata tcacgcaggt gataatgact
     8401 tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca ggtaaatagt
     8461 cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc aaatcatccg
     8521 tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat
     8581 gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg
     8641 gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcgctt
     8701 cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtga
     8761 atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa tgcacctgtt
     8821 ggatgcactg gcgtgtcaac gggccaacca tactttcgct ttttttctgg tacacgccac
     8881 cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg
     8941 tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg gcattgctgc
     9001 tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga
     9061 gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca ctatcaagcg
     9121 aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct
     9181 ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac
     9241 cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct gta
//
LOCUS       DAZPBM010000157         9244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPBM010000157 DAZPBM010000000
VERSION     DAZPBM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.158"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHUZQ_003436"
     CDS             complement(152..487)
                     /locus_tag="ACHUZQ_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1027049.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHUZQ_003437"
     CDS             complement(491..835)
                     /locus_tag="ACHUZQ_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1027050.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUZQ_003438"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUZQ_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1027051.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUZQ_003439"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUZQ_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1027052.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHUZQ_003440"
     CDS             1257..1376
                     /locus_tag="ACHUZQ_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1027053.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUZQ_003441"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUZQ_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1027054.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUZQ_003442"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUZQ_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1027055.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUZQ_003443"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUZQ_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1027056.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHUZQ_003444"
     CDS             4176..4544
                     /locus_tag="ACHUZQ_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1027057.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUZQ_003445"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUZQ_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1027058.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHUZQ_003446"
     CDS             5295..5588
                     /locus_tag="ACHUZQ_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1027059.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHUZQ_003447"
     CDS             5660..6319
                     /locus_tag="ACHUZQ_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1027060.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHUZQ_003448"
     CDS             6411..6857
                     /locus_tag="ACHUZQ_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1027061.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHUZQ_003449"
     CDS             complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHUZQ_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR1027062.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
     gene            8348..8767
                     /locus_tag="ACHUZQ_003450"
     CDS             8348..8767
                     /locus_tag="ACHUZQ_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1027063.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            8767..>9244
                     /gene="umuC"
                     /locus_tag="ACHUZQ_003451"
     CDS             8767..>9244
                     /gene="umuC"
                     /locus_tag="ACHUZQ_003451"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1027064.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT         2378 a         2050 c         2146 g         2670 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca gtcataatca
     7981 ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta ttaataaatt
     8041 ttacttcatc taattcatag ttagccgggc gggatgcgtc aatgtcttta tttctattaa
     8101 tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat tctatttttt
     8161 tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg ccacgtgagc
     8221 acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga tagcctgaat
     8281 cagtattgat ctgctggcaa gaacagatta ctgtatataa aaacagtata acttcaggca
     8341 gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt ttccgctatt
     8401 tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgtcg aacagcgcat
     8461 cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca aagcaagtgg
     8521 tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg atagcgctat
     8581 taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta cggtgaaaaa
     8641 attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact cgcccattac
     8701 catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg ttaaggcgat
     8761 gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg ccagctgtga gacggtgttt
     8821 cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg ttgcgttatc
     8881 gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg gttcaaacaa
     8941 aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga gctttacgca
     9001 gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt cgagatttac
     9061 agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga tctgactgat
     9121 tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt tggtatgggg
     9181 atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa atggcagcgg
     9241 caga
//
LOCUS       DAZPBM010000158         9075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPBM010000158 DAZPBM010000000
VERSION     DAZPBM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.159"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(507..1274)
                     /gene="fecE"
                     /locus_tag="ACHUZQ_003452"
     CDS             complement(507..1274)
                     /gene="fecE"
                     /locus_tag="ACHUZQ_003452"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR1027065.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="ACHUZQ_003453"
     CDS             complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="ACHUZQ_003453"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR1027066.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="ACHUZQ_003454"
     CDS             complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="ACHUZQ_003454"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR1027067.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="ACHUZQ_003455"
     CDS             complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="ACHUZQ_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR1027068.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="ACHUZQ_003456"
     CDS             complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="ACHUZQ_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR1027069.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="ACHUZQ_003457"
     CDS             complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="ACHUZQ_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR1027070.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="ACHUZQ_003458"
     CDS             complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="ACHUZQ_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR1027071.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8189..8257
                     /gene="ykiE"
                     /locus_tag="ACHUZQ_003459"
     CDS             8189..8257
                     /gene="ykiE"
                     /locus_tag="ACHUZQ_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR1027072.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8344..9041
                     /locus_tag="ACHUZQ_003460"
                     /pseudo
     CDS             8344..9041
                     /locus_tag="ACHUZQ_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1988 a         2511 c         2585 g         1991 t
ORIGIN      
        1 acacttttta gaaaaatcga tggtcagaaa ctggattagc aattccgttc cagggttgct
       61 tttgatttac gttggcgtct gatcattgat ttatcctcaa aagcccaacc tcattggtaa
      121 tgaaccagct ccgtgaatgt ccgctctggc acagagcgaa attttttgat ctcccccccc
      181 tgaaatctaa acttagtcat gtcacgtttt tgggtttcta aaattttaac ttcgcgtttt
      241 tcgttgccgt aagggttata cagaaatgtc cgttaagcag agttcaaaat tgattgccgt
      301 gatcacgact ggtttgaaag ccgcgcccaa gcctgtacag ctctggtttg cgttgattat
      361 gaacctgtca gcctaaagca agcggatgga cgatgagtat tggtaatctt tcagagtccg
      421 gaaaagttca gccccagtct gaacaggctt gctggcgcca gtccagtttc attcagtcgt
      481 ggtttggttc ttacggcctg tgcaatctac ctcattaggc acatcggcct gccagatacc
      541 ggctcggggt gtatttccgc ttccacgctg aatactgttc tcagcaatcc tggggtcatc
      601 acctcttctg gtgtgccttg cgccataaca tgtccgtttg ccattaccac cagttgatcg
      661 cagtaccggc tagcctgatt aaggtcgtgc agcacagcga ccaccgtttt cccctgagtc
      721 cggagttcgc ccatcaaccg catcaggtcc acctggtgat tgatatcaag ataggtggtt
      781 ggctcatcaa gtaatacaac gggcgtattc tgggccagga ccattgccag aaatgcgcgc
      841 tggcgctgac cgccggaaag ctcggttaac cgacgaacgg caagatgatt gatccgggtc
      901 tggttcatgg cgacattaac tcgtgcattg tcttcagcgg agagacgccc ccagagtgac
      961 agccagggat tacgaccata cgaaaccagc tcctggactg tgatcccctc tggcgttaaa
     1021 tggtgctgag gcagcagcga aagcctgcgg gccaactggc gcgatgagag catatttatg
     1081 ggattatcgc cgagaaatac ggtgccagac tgcggcatta aaagccgcga aaaacagttt
     1141 aacagcgtcg atttcccgca accgttagga ccgatcaggg cggtgatctt ccccgttggc
     1201 agtgagagtg aaacgtcgtt aagtaccttg tctgtcccgt aattgaccgt cagattttca
     1261 gttcgtaaag tcatttatcg cattctcaca agcaaccaga caaaccacgg cgcaccgata
     1321 atggcggtca gcacgccaac cgggagctcc agtgggggat gaataattct cgccagcaga
     1381 tcggcaacca ccaacagcaa cgcacctgtc agggccgaaa caggcagcag tctgcggtga
     1441 cgtccaccgg tgatgctacg catcatatgc ggcaccacga gaccaataaa gctaatcggg
     1501 ccgcaggcgg ccacgccggt agatgtcatg gcgacagcta gtaacaaagc ccagaatcgg
     1561 gtatagggca ccgacacacc gagcgtggtg gcgcgcgcat cgccgagtgc aaggaggtcg
     1621 agatcgcggc aaaaactcag gctcagcggc agaaataaaa tcatcagcgg gatggcaatc
     1681 ttcacaaagc tccagtcacg gccccataag ctgccggtca gccacagcag ggcgttgttc
     1741 acatcctgcg ggcgcgagag catcagataa tccgtcaggc tggcccagca tgcagaaagc
     1801 gccacgccgg tgagtgccag cttcatcggc tggtgggtct ttgccagcat cttcagcaat
     1861 atcaaccccg ccatgccgcc cgcaaaggcc agcagcggca gcaccatcac gggcagtgac
     1921 ggcataagaa gtagagcccc cacagaggcc aggctggcgg catggttaac gccgagaata
     1981 tccggtgatg ccagagggtt gcgcacaatc ccctgtatca gcacgcccgc cacggcgagg
     2041 gctgcaccga caaacagtgc cagcagcaag cgcggcagtc ggtactccat caatacataa
     2101 taatgctcgc gtccggcctg ccagtcggtc agcagcgcgc gccacggcac ggggatcact
     2161 cccatatgga gtgataacag cgcacagccc gccagggcaa gggtgatgaa aataaccagc
     2221 gcaattttca tcctcgcctc ctcacaagcc agacaaagca agggctgcca atcagcgcca
     2281 gcactgcgcc tgcgggcaga tctccgggga aggccagcgc gcgtgcgagt acatctgcca
     2341 gcagcatcag cgtggccccc agcagcatgc tcactggcag tacgttgcgc tgatcgaagc
     2401 ctgcccagaa gcgcgccaga tgtggcacca gcagaccgat aaacgccacc ggacctgcca
     2461 cgctgacgca cgcaccaacc agaagcagca ctaacatatt gatgaccaaa cgtagcctcg
     2521 tcaggttcac tcccagcgta tgggcggtgc tgtcgctgag gttgagcagg ttcagttgat
     2581 tcgccagcag caacacgaca gggactgcag tgaccaccac cggcaagagc tgccagacat
     2641 cctgccagcg ggcgtgggac actccgccag ccagccagta aaagatgccg taagcatgat
     2701 cttcggccag cagcagggtg atgcgggtaa ggcccataca aaaggccgac agcgcgatac
     2761 ccgcgaggat cagtttgttt ctgtcatggg tatgacgaaa tccgcctcct gcggtcatga
     2821 ccagcagcca gctcacgccg cccccgcatg ccgcgatgaa cgacagagaa tagcctgcaa
     2881 tcggcgtcgg actcagcgcg ctggtaagcg ccatagccag cgccgcgccg ctgttaatgc
     2941 cgagcagtga aggagaggcc attgggttgt gggtcagggt ttgcagcagc gtgcccgcga
     3001 gcgccaggct tgcgccgatc agaacggcga ccaggcttcg tggcaaacga aggttttgca
     3061 ccagcgcttc tggtagcgtt ggcgtgtgtc caggcagcag ggcgcgggtt gcatctgctc
     3121 cggaaacagg aatggccgag tagcaaaaca gactcagcca gaaaataata ataagtgctg
     3181 caacgggaag cccccacagc agcaccgggt gttttatcgc ggtcatttca caacggtaag
     3241 cggctgatgg tggaagattt ttaccgtgtc agcggcaata cgctctgcag caaaaatacc
     3301 gcgcatccgc gcccaggtgt tactgtcgac cgaagcaacc tgctgcttct gcgcggcggt
     3361 taacatctgc cagagcggat cttgttgcca gcgtttaaca atgctctctt cgcgatagtg
     3421 ggcaaccagc agccaggcag gattgaccgc cagcagttgc tccaggccga tggacggcat
     3481 ggacgcaccc gccatcgcag cgggaacgtt cagccccaga gaggccagca cgctgccggt
     3541 ccaggtctcc tgagtatgca ggttgaattg ctgttcgcgt gatgtgccaa aggccacgcg
     3601 tgtccctttg ggaagctggc tggcccactg cgccatcctc tctttatgtt gttccagacg
     3661 tgcctgcatc tctcgctttt tacccaccat ttcgccgatg atagccgcag attgcaaatt
     3721 ttcagcgtag gtttcgttgc gggacttaag cagcagtacc gacgcgattt gctgcaaggc
     3781 gatgtaaacc cccgcatggc gactgctgtc ggcaatgatc aggtctggtt tcagagcggc
     3841 aatggcttcc aggctcggct gcgcgcgcgt tccgacggac tgccacggtt tcaggtgcgc
     3901 acgcacttcg ggcaggatgc gttttgcatc gttatcgtcg gcaataccga tcgggctgac
     3961 gtccacggcg gccagcgcat cggcgaacga gagttccagc accacaatcc gttgtggcgt
     4021 tttttcgagt gtaaacgtgc cgtgttcgtc ctgaaccgtg gcggcaaagg cgtggctgat
     4081 caccagcagc aggcctgcaa aaagaaaacg gataaatgcc aacataatca cattccagct
     4141 aaaagcccgg caagccgggc gttaacacat cagaacttca acgccccctg catatacagc
     4201 gtgcgcggct ggcctgcata gatgcctttg ttgttgtcgt cataagagcg gatgaagtag
     4261 tcctggtcga agatgttttt cacaccgaat gccaggttca gatctgccat ctgcgggcca
     4321 aagtcataca ccacgcgtgc gccccagagc atgaagccgg gaatgcggcc ggtactgccg
     4381 tcggcgctct ctttcaccgt attggcgtta tccgcaaact ggctggactg gaaatcgctg
     4441 ttcagattga acatccagtt tcctggcttg tagtccacgc ccagcgtgcc tttatgtttc
     4501 ggggagaatg gtaccagatt gccgtaggtg tcgcctttct cgcggatttc cgcgttcaca
     4561 tacgcatagc tggcgtagat ggaaacgtta tcaagcgttg gcgttagcgt acccagatcg
     4621 taacgtgcct gcgtttccag cccggtatgg cgcgttttgc cacgtgcagt gacggtgtcg
     4681 ttggtctggt tggagtcgta ctgattgtta aagttaatca ggaacagccc catttccgcc
     4741 gtcagcgcgc cgtcgtcgta gcgggtaccg agttcccagg ttcgcgcttt ttccggttcc
     4801 acattgccgc tttgcacagc cttgccaatc tggctgtact gtacggtgcc gaacgagcct
     4861 tcagtgtttg cataaagatt ccagctgtca gtcaggtgat agagcacgtt caacgccgga
     4921 agcggtgcgt tatagctcac ttcttcgtgc gtgcctgtga tggcgttgtt ctggtatgac
     4981 tcgatatgtt cgaaacgcat acccggcgtg atggtccagt tgccgatgtc gattttgtca
     5041 tccagatacc aggcgtgcgc ctcggtgccg gaacgcgtat cgcggtcgta agggcttgag
     5101 ccggacggca actgcccgct gctggtggcg gtgtagtaac gcatttcatg cgttgattca
     5161 ttcacatagc gatagcccac gcccacttcg tgcgcggaag ggccgatcat aaagatctgg
     5221 ctgtagcgtg gctcaatacc gcgcacccag tagttacgcg gcgagagggt gatgcgtttg
     5281 ccttgctcca ggtagccgct gcgcagggtt tgggtgtaga acccctgaat gttgaattta
     5341 tgctggctgt ctggctggaa ctggtagccc aggctcgcca gcttgcgacg accccagaag
     5401 cggtcatacg ggcgggtgga ttgccagcga tcggcgtcgt aatccgcgcg agacaggcca
     5461 ccgggcatgt cggcttcacc gtcgtaatat tgcagcaggc tgttgaaggt gtgcacctca
     5521 tccggcgcat atttgctttt cagcatcagg tcgtcgatgc gggtggcgct gtgctcgcgc
     5581 cagtcactgc cgcgcgtgcc ggagtagagc agcgcggtgc caaaaccgtt gtccgctgtg
     5641 ccgcccacca tcaggttgtg cgtctctttc gggttgtttt gtgaagaggt tgggctgagc
     5701 tgaccttcca cgcccgcctc gataccaaag tcctgcggaa tggcacgggt aacaaagttc
     5761 accacgccgc ccacgctctg cggtccgtaa cgcaccgcac caccaccgcg taccacgtca
     5821 atggcatcca tgttgccgag cgaaacggga gccagtgaaa gctgcggctg accgtaaggg
     5881 gcgaagggga cggggatgcc gtccatcagg acggtcgagc ggctggcgag gcgcgggttc
     5941 aggccccgga tgccaaagtt catcgccagg tcgtggctgc cggtgccgtt gttttccggc
     6001 gcgctgacgc cagggatgcg gttaagtacc tcacgcatgg tggttgcgcc ggttttggcg
     6061 aaatcctcac ggcggatcac gtcacgcgcg ccagcatgtt caaatacgtc gttttcacgc
     6121 gcatcaccca gccagtcgcc gaccacggtc agggcatctt cttttggtgc gggcgcgggc
     6181 tccagcgtcc agctgttatt tcccagcggt tttacctgca gtccgctgcc gtccagcagt
     6241 tgttgcaggc cgctctcgac gtcgtaatcg ccgtgcaggc cgttgctctg cttgccgcgc
     6301 gtcaggctgg cgtcaaccga gagggtaaat ccgctgtgtg cggcatactg attgagcgct
     6361 ttatcgagcg atcccggtgc gatattaacc tgtgcagcaa aagcggaaaa cgagagaccg
     6421 gccagcggca gcaggctcag gcgaatggcg ttaaccaaag gtgttgtttt acgaaaaacg
     6481 cgtaacggcg tcataccttc cccatcatca tttttgttgt gttcagctat gagtcgaacg
     6541 agaagggtaa aaaggacaat cgaaataaga attattttcc ttacagtggt gaaatgttta
     6601 tccagtaccg cgtaatagac tgaattttaa cgggaagcgt ttgcgcgata acgttcagga
     6661 tcgcatcggt atttttcagc gggaacgtcc cgctcaggcg cagcccggca acggcgggat
     6721 cgcagcgcag cacgccgttg cggtaacggg ttagcgtggc tatcacctca cccagcggtt
     6781 tatcgctgaa gctcaggatg tccttcgtcc agcttgtact ctcgtcatcc agcggtttca
     6841 ctgcgccaaa ctcagaggcg ctgaactgca ggctttcacc agcgttcacg atgcgttttt
     6901 gcgcgggggc actggcgaga agcacttcca cggcgtgctg ctgcacgtca agctgcgtga
     6961 aattatcctg ctggcggacg gtaaattctg tccctaaagc agtgagctgg ccctgacggg
     7021 tcaggacacg gaaggggcgt tgcagggcat ctttcgcggt agtaatggcg atttcaccgt
     7081 accagagccg gacggtgcgc tgatgcgcat caaaacgcat atccgccgcg ctttgggtat
     7141 tcagcgtgag cagggagcca tcttccagtt gctgacggct gactgtgcct ttggcggtgc
     7201 ggtaatctgc ccgcagacct tcgccggttt ccgactgcca gagctgccag cctccgccag
     7261 cgccgagcaa cagcagtaat cctttcatca cgtgacggcg ggtgaggcgg gtatcgtgca
     7321 acgcccggct ggcaacgtcg ccaggcacac cgccaagctg gttgcgcagg ttttcaacct
     7381 gctgccaggc ccactggtta tcctgatcct gttcatacca ctgttgccag cgcgcttcct
     7441 gttgtgggct gacgcgctcg ccgcttagca cggcatacca gtgggaagct gaacgcagcg
     7501 cctgacggcg ggaatcggtt aacaaaggat tcataaccca tactccagac ggaacagcag
     7561 gcagtgctcg acggctttcg ccacgtattt tttcacggag ctgatggaaa caccgagttt
     7621 gtgcgcaatc tcgctgtatg tcagaccatc cagttgcgaa agcagaaacg cttcacgtgt
     7681 tttgccgttt agcccgtcca gcatgctgtc gaggagttgt agggtctcga gttggctttc
     7741 gcgttcctca ggtgaaggcg ctcccccctc cggcataagc gccagcatct ccagatacgc
     7801 tttttccagc gcgtttcggc gaaacaggtc caccatcacg cgtttggcga tagtgcagag
     7861 gaaggagcga ggatcgcgga tcgtcgagag cgtttcgctg accattaccc gcaaaaaagt
     7921 gtcctgggca atgtcatctg catcaaaagc agactggagt ttgcgcgtca gccagctttt
     7981 caaccagccg tgatgtgtgc cataaagcga ctcgaatgtt aaggaagctg tggtagtggc
     8041 gcggtcagac atgcggagtg catcaaaagt taattatcac gtagccatat taatatgaga
     8101 atagttatca ttacaattgg aaataaaatt gtttccaata gacattttta acatgttgtt
     8161 tttctaagtg ttataaggta ggtataaaat gggatggagc ctctgcttct ggcatgtgtc
     8221 ggtcagaatg actcatgatg tggtctgcta ttattgacat cctcactgcc ctaaaggata
     8281 gggatttcgg taatgctgtc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     8341 tgcgtggctt ccatttccat cagatgtcct tcctgctccg ctactgaagg cgtggtgcgt
     8401 aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcattgccg taaaacatgg
     8461 caactacagt tcacttacac cgcctctcag cccggtaagc accagaaaat cattgatatg
     8521 gccatgaatg gcgtcggatg tcgcgccagt gcacgcatta tgggcgttgg cctcaacacg
     8581 gttttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata caaccgggca
     8641 gtgatgtgat tgtctgcgct gaaatggacg aacagtgggg ctacgtcggt gctaaatcac
     8701 gtcagcgctg gctgttttac gcgtatgaca ggatacggag gacggttgtg gcgcacgtct
     8761 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
     8821 tcgtggtata gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc
     8881 acgttatcag caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac
     8941 atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca
     9001 aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attacctcct
     9061 cccgaactca aaact
//
LOCUS       DAZPBM010000159         8970 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPBM010000159 DAZPBM010000000
VERSION     DAZPBM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8970
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.160"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            248..1135
                     /gene="rhtA"
                     /locus_tag="ACHUZQ_003461"
     CDS             248..1135
                     /gene="rhtA"
                     /locus_tag="ACHUZQ_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1027073.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            1434..1937
                     /gene="dps"
                     /locus_tag="ACHUZQ_003462"
                     /gene_synonym="pexB"
     CDS             1434..1937
                     /gene="dps"
                     /locus_tag="ACHUZQ_003462"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1027074.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            2341..3087
                     /gene="glnH"
                     /locus_tag="ACHUZQ_003463"
     CDS             2341..3087
                     /gene="glnH"
                     /locus_tag="ACHUZQ_003463"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1027075.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            3226..3885
                     /gene="glnP"
                     /locus_tag="ACHUZQ_003464"
     CDS             3226..3885
                     /gene="glnP"
                     /locus_tag="ACHUZQ_003464"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1027076.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            3882..4604
                     /gene="glnQ"
                     /locus_tag="ACHUZQ_003465"
     CDS             3882..4604
                     /gene="glnQ"
                     /locus_tag="ACHUZQ_003465"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1027077.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            4721..6946
                     /gene="ybiO"
                     /locus_tag="ACHUZQ_003466"
     CDS             4721..6946
                     /gene="ybiO"
                     /locus_tag="ACHUZQ_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR1027078.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(6943..7869)
                     /gene="rlmF"
                     /locus_tag="ACHUZQ_003467"
     CDS             complement(6943..7869)
                     /gene="rlmF"
                     /locus_tag="ACHUZQ_003467"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1027079.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            8145..8405
                     /gene="mcbA"
                     /locus_tag="ACHUZQ_003468"
     CDS             8145..8405
                     /gene="mcbA"
                     /locus_tag="ACHUZQ_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1027080.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            8670..>8970
                     /locus_tag="ACHUZQ_003469"
     CDS             8670..>8970
                     /locus_tag="ACHUZQ_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1027081.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         2137 a         2272 c         2249 g         2312 t
ORIGIN      
        1 agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt
       61 aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac cagactaatg
      121 attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc
      181 aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga tatttgtggg
      241 agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct
      301 cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt
      361 gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt
      421 ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt tgttttacgg
      481 cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag taccgctggg
      541 tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct cttctcgtcg
      601 cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct
      661 ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc
      721 ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg gccctgcgac
      781 ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc
      841 tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg ctattctctc
      901 gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacggacatt
      961 tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga ttttcctcgg
     1021 agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg
     1081 gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta attaaattta
     1141 catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc tctacaccgt
     1201 ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc gagcagataa
     1261 ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt ttgtttttca
     1321 cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac atagccggtg
     1381 ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa attatgagta
     1441 ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac gatgtctccg
     1501 acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag tttattgatc
     1561 tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc attgccgtac
     1621 atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc atggcagaac
     1681 gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac agcaaaaccc
     1741 cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa gaactggctg
     1801 accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg
     1861 acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg tggtttatcg
     1921 agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc gataaaagcg
     1981 gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta tttagtgcat
     2041 ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc acacaatcgt
     2101 tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt tgagtgcaca
     2161 attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt gccctataaa
     2221 tcgtgcatca cgtttttgcc gcatctcgaa aaatcaagga gttgcaaaac tggcacgatt
     2281 ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct
     2341 atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc ggtttcttct
     2401 catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc gtttgaattt
     2461 aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat cgctaaagag
     2521 ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc ggcactgcaa
     2581 accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg taaaaaagcg
     2641 atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa agctaacaat
     2701 aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgttaa gagcggtact
     2761 ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca gttcccgaac
     2821 atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct gcacgatacg
     2881 ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac
     2941 tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac
     3001 aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga aatctacaaa
     3061 aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaagcgca ctggcagttc
     3121 cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg gggccattaa
     3181 ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca gtttgactgg
     3241 agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac cctgtggatt
     3301 tcggtcctcg gtctggctgg cggtctggta atcggattgc tggcaggttt tgcacgcacc
     3361 ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaggtgat ccgcggcaca
     3421 cctatcgtcg tccaggtgat gtttatttat ttcgccctgc cgatggcgtt taacgactta
     3481 cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg cgcgtacatt
     3541 gcggaaatca cgcgtggtgc ggtgctgtct attcacaaag gttttcgtga agcaggactg
     3601 gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt
     3661 cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac ctcgctgttt
     3721 attgtgatcg gcgtggcgga actgacccgt caggggcaag aaattattgc cggtaacttc
     3781 cgcgcccttg agatctggag cgccgtggcg gtgttctatc tgattattac cctggtgcta
     3841 agctttattt tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg
     3901 tctccaagca ctttggccca acccaggtgc ttcacaatat cgatttgaac attgcccagg
     3961 gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg ctgcgctgca
     4021 tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg aaggttaacg
     4081 atccgaaagt tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc cagcagtttt
     4141 acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta cgcgtgcgtg
     4201 gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg
     4261 cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag cgtgtggcga
     4321 ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc
     4381 ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct gaagaaggga
     4441 tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct tcgcggctga
     4501 tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg atcaagaacc
     4561 cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc tgcctccctt
     4621 tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc ccccagcatc
     4681 tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga tcctgttcat
     4741 cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg ttacaaccac
     4801 aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac aaaaaaaagc
     4861 ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag aattgatcga
     4921 ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga tcgtgccgcc
     4981 gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc gccattatgg
     5041 tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct ccccgcataa
     5101 gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt tagcggtatt
     5161 agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctgtatc gcaaaatggg
     5221 tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc cggcgatgat
     5281 tatcggggcg tttattatcg acctgctgtt actggccctg acattatttg tcggccaggt
     5341 attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa gtttgtttct
     5401 caacgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt tttgcccaaa
     5461 cgtggcggag ctgcgcccgt tcacgattca ggacgagagc gcccgttact ggagtcgtcg
     5521 cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggccgtgc cgattatctc
     5581 taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc tgtgcatgac
     5641 cgtctgggcg ttgtacctga tctttcgtaa taaaaaagag attacccagc atttgctcaa
     5701 ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg cgctggtgtg
     5761 gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt tcgatccggg
     5821 caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta ttggtatcgc
     5881 agcgtttgtt tccggtatgt tttcccgctg gctggcaaaa accatcactc tctcgccaca
     5941 tactcagcgt aactatccag agttgcaaaa acggctgaat ggctggcttt cggcggcgct
     6001 gaaaacggcg cgtattctga ccgtctgcgt ggcggtaatg ttgctgttga gcgcatgggg
     6061 attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg tagatatcct
     6121 gatccgtatc gcacttattc tttttttctc ggcggttggc tggacggtgc tcgccagttt
     6181 gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg cccgtacgcg
     6241 taccctgctg acgctgttcc gtaacgcgct ggcggtgatt atcagtacca tcaccatcat
     6301 gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg ccggggcatt
     6361 aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatatcatta ccggggtgtt
     6421 tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc cgttgaccgg
     6481 cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg gggcgtatca
     6541 catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta ttggttcggt
     6601 agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc aggcactgaa
     6661 agatgcggta gcggaattaa tggaaaacga agaaattcgc gggctgatta ttggtgaacc
     6721 gaattttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg tttcgttcac
     6781 cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg tgaaaaaaca
     6841 tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc ctactccggg
     6901 cgcgaccccg gctgaaccgt taccgccggg ggagccaacg ctttaacgct ggcgattgac
     6961 aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac tctgcttttg
     7021 cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca gagcacgata
     7081 caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca cctgcttcgc
     7141 gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac cttcacacca
     7201 caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc ccaggttacg
     7261 gcgtttacgc tcactacctg cccgcgctgc agcggcggaa tcgtggaatg gcgggttaca
     7321 caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaattg ccccactctc
     7381 tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga taatcgcctg
     7441 cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc catattcatg
     7501 tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa tgctggcatt
     7561 cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa tataatccgc
     7621 ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg ctacggcgta
     7681 aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg caaagtctac
     7741 gctttgctcc ccggcgggtg taagcgtgag gaattgcctg agttcaggat tgacctgaca
     7801 aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc ccggtttctg
     7861 ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac gcgacagggt
     7921 aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt ttatggcaat
     7981 tcatcataaa aatcagcatt atgcatgtca ttaatttgtc ttattttgta aaaatatatc
     8041 gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca tttgcatcgc gcaggcgacc
     8101 tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa aagtgcctca
     8161 cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc gcgcaaccga
     8221 tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca actggcgcat
     8281 caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgaacaa ggcgcgaaag
     8341 gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca accatctaca
     8401 aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct gttttgtaat
     8461 caaaaaacat caaatatgaa ctcaatgtaa ataaatgtat ttctttttcg cgcaatgggt
     8521 gatagaaaat cgcgccaagt gataatgctt atcaaaatta ttatcacttg cacgaacact
     8581 atcacgggat taacagtggc atcgcatccg cagagatgct ttctcgtggc agtgaaaatt
     8641 tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc cctgccagac
     8701 aatttcattc gctcacgttc tttgccggtc tttgtattgg catcacgcct gtggctcagg
     8761 cactcgccgc cgaagggcaa actaacgcgg atgacacgct ggttgtcgaa gcatcaacgc
     8821 cttcgcttta tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg gtagcggata
     8881 ctacccgcac gatgacggtg atttctgaac aagtgattaa agatcagggc gcaaccaacc
     8941 ttaccgacgc gctcaaaaac gcccccggcg
//
LOCUS       DAZPBM010000160         8885 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPBM010000160 DAZPBM010000000
VERSION     DAZPBM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.161"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            168..1566
                     /gene="ulaA"
                     /locus_tag="ACHUZQ_003470"
                     /pseudo
     CDS             168..1566
                     /gene="ulaA"
                     /locus_tag="ACHUZQ_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1582..1887
                     /gene="ulaB"
                     /locus_tag="ACHUZQ_003471"
     CDS             1582..1887
                     /gene="ulaB"
                     /locus_tag="ACHUZQ_003471"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1027082.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1897..2358
                     /gene="ulaC"
                     /locus_tag="ACHUZQ_003472"
     CDS             1897..2358
                     /gene="ulaC"
                     /locus_tag="ACHUZQ_003472"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1027083.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2372..3022
                     /gene="ulaD"
                     /locus_tag="ACHUZQ_003473"
     CDS             2372..3022
                     /gene="ulaD"
                     /locus_tag="ACHUZQ_003473"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR1027084.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            3032..3886
                     /gene="ulaE"
                     /locus_tag="ACHUZQ_003474"
     CDS             3032..3886
                     /gene="ulaE"
                     /locus_tag="ACHUZQ_003474"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1027085.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3886..4572
                     /gene="ulaF"
                     /locus_tag="ACHUZQ_003475"
     CDS             3886..4572
                     /gene="ulaF"
                     /locus_tag="ACHUZQ_003475"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1027086.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4669..5220
                     /locus_tag="ACHUZQ_003476"
     CDS             4669..5220
                     /locus_tag="ACHUZQ_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR1027087.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5295..5570)
                     /gene="yjfY"
                     /locus_tag="ACHUZQ_003477"
     CDS             complement(5295..5570)
                     /gene="yjfY"
                     /locus_tag="ACHUZQ_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1027088.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5897..6292
                     /gene="rpsF"
                     /locus_tag="ACHUZQ_003478"
     CDS             5897..6292
                     /gene="rpsF"
                     /locus_tag="ACHUZQ_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1027089.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6299..6613
                     /gene="priB"
                     /locus_tag="ACHUZQ_003479"
     CDS             6299..6613
                     /gene="priB"
                     /locus_tag="ACHUZQ_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1027090.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6618..6845
                     /gene="rpsR"
                     /locus_tag="ACHUZQ_003480"
     CDS             6618..6845
                     /gene="rpsR"
                     /locus_tag="ACHUZQ_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1027091.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6887..7336
                     /gene="rplI"
                     /locus_tag="ACHUZQ_003481"
     CDS             6887..7336
                     /gene="rplI"
                     /locus_tag="ACHUZQ_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1027092.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7448..8419)
                     /locus_tag="ACHUZQ_003482"
     CDS             complement(7448..8419)
                     /locus_tag="ACHUZQ_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1027093.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8472..>8885)
                     /locus_tag="ACHUZQ_003483"
     CDS             complement(8472..>8885)
                     /locus_tag="ACHUZQ_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1027094.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         2139 a         2170 c         2359 g         2217 t
ORIGIN      
        1 taattgtttc gttgtgtgaa tcccactcta tccgtgtgga attatttgcg ggtcgcgtca
       61 catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca
      121 ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct
      181 acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta
      241 ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca
      301 cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca
      361 ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaaacgg cgcaatttcc
      421 gatacttacg cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta
      481 ggttacgcgg tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt
      541 accggtattc gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt
      601 gccgttacgt tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt
      661 ctggtttcgc tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg
      721 acggacggct gcggtttctc catcggtcac cagcaacagt ttgcatcatg gactgcctat
      781 aaagttgcgc cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc
      841 tggctgaata ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt
      901 ggtgccattc tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac
      961 tggacggtct atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact
     1021 cagggtgtgc gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc
     1081 ctgatccctg gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgcgccgaac
     1141 gccgtggtct ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg
     1201 gtcgcctgcg gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac
     1261 gccaccatcg gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt
     1321 ctggtgatgg ggatgatcga aatctttggt tgcgtctggg cggtgaaact taccggtatg
     1381 agtgcctgga tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc
     1441 ttctccatcg gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc
     1501 ttcgctggcc gcgcactgcg cgcagaagaa gatgcagaaa aacaactggc agaacagtct
     1561 gcttaataag gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa
     1621 ggcagttcca tgatcatgag gatgaaagtg gaccagtttt taacccaatc aaacattgac
     1681 cacacggtaa atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc
     1741 atcatcgctt ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt
     1801 ggcgtgcgca acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa
     1861 gcgcatttcc cgcaggatgt gaagtaagga cgcgccatga aattacgtga ttcgctggcg
     1921 gagaataaat ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc
     1981 ggcgttgacc tgctggtggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     2041 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     2101 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     2161 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     2221 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     2281 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     2341 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     2401 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     2461 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     2521 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     2581 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     2641 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     2701 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     2761 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     2821 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     2881 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     2941 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     3001 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     3061 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     3121 gttgggcttc gattttgtcg agatgtcggt agatgaaact gacgagcgcc tgtcgcgcct
     3181 cgactggagc cgcgagcagc gtctggcgct ggtgaatgcg attgttgaaa ccggcgtgcg
     3241 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     3301 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     3361 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     3421 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     3481 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     3541 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     3601 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     3661 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     3721 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     3781 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgcgtgattg
     3841 ggtgaaagcg cgcatggcca aagcaggcac ggtggaggcg gcataatgca aaagctaaaa
     3901 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacctgg
     3961 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     4021 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     4081 gaaggggagt atcgcccgtc ttccgacacc gcgacgcatc tcgaactcta ccgtcgttac
     4141 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ctactgcatg ggcgcaggcg
     4201 gggctggcga tcccggcgtt aggcactacg cacgccgact acttctttgg cgacatcccg
     4261 tgtacgcgcg gattaagcga agaagaggtg cagggcgagt atgaactgaa caccggcaaa
     4321 gtgattatcg aaacgctggg tgacgccgag ccgctgcata cgccggggat tgtggtgtat
     4381 cagcacggtc cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtggtg
     4441 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaacccaca actgaatcac
     4501 atcgacagct tcctcatgaa taaacacttc atgcgtaagc acggtcctaa cgcctattac
     4561 gggcagaagt aagcagtgcg ccccggaatt attacctcct gcgggatgta attggtctga
     4621 ttccggggcc gcttcagtta aaaaatcgta taaacaacag gtgtttttat ggctatcaat
     4681 ggattgtttg tcagaacgga tccgcaacgt cgccgctatg gcgttgcgtt acttgtgggt
     4741 gtactttcag gaattatttc agcatttgtt aaatggggag cggaagtccc cttgccaccg
     4801 cgcacatttt ccggtggtcg cgatgaattt aacccgccgt atatctttct acgggattat
     4861 ttaggtatcg acccaaccca aactgtgtat acattctcgg aacatattat caacccggtg
     4921 atggtgacgc atatcatctt ctcactggtt tttgcgatag ggtattgtat tgtcgcggaa
     4981 attttcccga aagtgaaatt atggcagggg atattagcag ggcttattgt gacagtggca
     5041 gtccacggta tattttgccc ggcgctgaat ttaacgccgc cgctcactca attgcctttt
     5101 gacgaatatg cctctgaaat cctggggcat attttctggt tctggataat agaaattatg
     5161 cgccgcgact tacgtaatcg cattactcat gagccagatc cggaagtcgc aatccggtga
     5221 ataaccgcat aaaaaagccc cctgcgaagg gggcaaagca aactatggca atgtttcgtt
     5281 ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc gttactcccc
     5341 ggttcccgca tcagaattac gtggtagtac gttgcgccct gcgcatctgt ctcttcattt
     5401 aatgcctgac gcgcttcgct ttcagtggcg aaattatgat tgatataaat cacgcctaag
     5461 ctttgcacat catccatgtt tctggcctgg tggttattaa tttcaatggc tgcccatgta
     5521 tttgcactta gcaaaagcac agccagaagg gctaaaactc gactgaacat agatacctcc
     5581 tcgacggctg actttgtgtg ctctcctctg tgatgatctt ctgatttaat tttaatcaat
     5641 gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ccatgatgaa
     5701 gtgtgatgaa ctccaaatca gcggtttaga ggttaattgc gaaaggggag atttatttcg
     5761 gctctgccct tgagtttagc ggggcataca agtactataa cgcgtcattt ttcagccgac
     5821 ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc
     5881 cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag
     5941 cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa
     6001 gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca
     6061 caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga
     6121 aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc
     6181 tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga
     6241 tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat
     6301 gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc
     6361 atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg
     6421 ttttcaccgg caggcgtggt gccaaatgcc cgttattgtt agcggacacg aaaaccaggc
     6481 cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca
     6541 caaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga
     6601 ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg
     6661 cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg
     6721 aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc
     6781 tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc
     6841 agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct
     6901 gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta
     6961 tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga
     7021 attcttcgaa gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagctgc
     7081 taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg
     7141 cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc
     7201 agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac
     7261 tggcgagcac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa
     7321 cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca gtggtcggcg
     7381 ttttgctttt gtagcgagca cttgcagata tcgataaggg ccacaataag cagccctacg
     7441 aagataatta cttagctgcc tgaatatcag ccagcattgg ggcaagggct gggttgtcct
     7501 tagcgaagtt tgcgtacatt ggctgtactt tctcgataaa cggagcttta tctacctggt
     7561 agactttcac gcccatttta tccagttttg caaattcttg tttctcttcc tcgctccaca
     7621 agtctttcat gtagctcatt gattctttag ctgcttttaa tataactgcc tgattttccg
     7681 ggctaagttt atcaaacgca gcattgctca tcagaaccac atcgggaacc atagtgtgtt
     7741 cgtcaaggct gaaggatttt gccacttcac cgtgacgcat ggtggtcagc gccatcacgc
     7801 tgttttctgc catatcgacc acaccctgct gtaaggctgt atacaactcg ccgtaatcca
     7861 gtggcgtcgg gttaccgccc atgactttga tcatttcaac tgcgccaggg cttggctgga
     7921 cacggacttt catgcctttc agatcgtctg gtttcagtac tggtttattg ccatagaaac
     7981 tgcgggcgcc tccatcataa aaagtcagcc cgaaataacc tttgcttttc gttgagtcga
     8041 ggatttttct cccgatatcg ccctgcatga cctgataata atgatcacga tcgcggaaca
     8101 aataaggcag gctaaataat ttataggaat tttcaaatgc ttccatttct gccgcattgg
     8161 tttttaccag tggaatagcg ccagaacgaa tcagctccat tgtttctcgt tgagtaccca
     8221 atgttccatt tggataaata cgaatagtaa tatcaccgtt agagtactct ttgttctttt
     8281 tcgcaaagaa ctccatcgct ttatgaacag gatgagactt atcctgatta tgactcagtt
     8341 ttaatgtcac tgcccaggac tggcttgcgg ataacataag aacagcaccg ataatgccag
     8401 cggatacttt tattttcata gatgttcctt ttctaataaa tatggcggca ggtgccgcca
     8461 gtgaatgcgc atcagtagcc cagtgctttt ggcaatgcca tactgatttg cggaatatag
     8521 gtcaccattg ccagcaccaa taccatcacg gcatagaatg gcagcatagg tttgataatt
     8581 ttattaatcg gtaatttaga tacactacaa ccgacaaata acacactgcc tacaggcgga
     8641 gtacaaatac cgatacacat gttcagtacc atgacaatac caaagtgaat cgggtccatg
     8701 cccagttcgg tgacaacagg taaaaatatc ggcgtaaaaa tcagtaccgc cggtgtcata
     8761 tccataaaag tgccgataat caacaggata atattgataa taagcaggat tattattggg
     8821 ttgtcagaaa tagtgttaag caaatcgaga atcaggaagg gaacgtcagc gtttgacatt
     8881 gccca
//
LOCUS       DAZPBM010000161         8792 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPBM010000161 DAZPBM010000000
VERSION     DAZPBM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.162"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..546
                     /gene="ykgH"
                     /locus_tag="ACHUZQ_003484"
     CDS             <1..546
                     /gene="ykgH"
                     /locus_tag="ACHUZQ_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR1027095.1"
                     /translation="VNKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQR
                     ITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDE
                     IQIPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIV
                     ISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(789..1484)
                     /locus_tag="ACHUZQ_003485"
     CDS             complement(789..1484)
                     /locus_tag="ACHUZQ_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1027096.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1477..2904)
                     /locus_tag="ACHUZQ_003486"
     CDS             complement(1477..2904)
                     /locus_tag="ACHUZQ_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1027097.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2915..3634)
                     /locus_tag="ACHUZQ_003487"
     CDS             complement(2915..3634)
                     /locus_tag="ACHUZQ_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1027098.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4162..5016)
                     /gene="rclR"
                     /locus_tag="ACHUZQ_003488"
     CDS             complement(4162..5016)
                     /gene="rclR"
                     /locus_tag="ACHUZQ_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR1027099.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5242..6567
                     /gene="rclA"
                     /locus_tag="ACHUZQ_003489"
     CDS             5242..6567
                     /gene="rclA"
                     /locus_tag="ACHUZQ_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR1027100.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6676..6912
                     /gene="rclB"
                     /locus_tag="ACHUZQ_003490"
     CDS             6676..6912
                     /gene="rclB"
                     /locus_tag="ACHUZQ_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR1027101.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6924..7517
                     /gene="rclC"
                     /locus_tag="ACHUZQ_003491"
     CDS             6924..7517
                     /gene="rclC"
                     /locus_tag="ACHUZQ_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR1027102.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7677..8546)
                     /locus_tag="ACHUZQ_003492"
     CDS             complement(7677..8546)
                     /locus_tag="ACHUZQ_003492"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1027103.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2431 a         2110 c         1873 g         2378 t
ORIGIN      
        1 gtcaataaag ataatataaa agtgagttac agcctaaaaa taaaccagac aacaccaggc
       61 atacttgtta actgtgatag caataataac tttgcctgtc agaccacaat gactgaagat
      121 gttatacagc gaattactac attttttcac accagcccag atgtcaagaa cagagaaata
      181 aagctggaat ggtcaggaga taagagagat ttaccaactg ctgaagcgga aatatctcgc
      241 gtgcaggcgt ctattatcaa atggtatgcg tcagaatacc ataatggcag gcaagttctc
      301 gatgagatac aaataccttc agcaattaac agtgagcttt atacaaaaat gatatacctg
      361 accaggaact ggtcattgta tccgaacggt gatggctgtg taactataag ctcaccagaa
      421 ataaaaaata aataccctgc tgccatttgc ctggctctgg gattttttct aagcattgta
      481 atttctgtaa tgttttgcct tgtcaaaaaa atggtagatg aataccaaca aaactctggg
      541 cagtaataaa cagcattctg atagagagtg cctgtataaa gctgaagtga caatgaccgg
      601 gaagggaacc cgttttcagg aatggatgcc tgtctttttc actgtaaata tcctggcatt
      661 actaatgcca ggatatttac agaagagcag cataaccatg acggaacggg gttaagtcag
      721 gttgtcctta acgctatttt caacctcgta tgccttcttc aggtttatgt tcagatctca
      781 tatctctctc agcaatcctc aataatcaga tacaccgctt tcaccggacc atgaaccccg
      841 acgactttga taagctcaat atccgccgtt gagctgggac cactgatgat gttaatacat
      901 gacggcattc gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag ttgcgctaca
      961 cgcggcagga ttgtgctttt acgcaggata aaaagagaag attccgggag caggctcaat
     1021 gaacgcccgc gctcgacggc ggaaaaaaga accacgcctc ccgattcggt taaaccatat
     1081 tcagcataca cgacacccac tttagcctgc tctgcctgcg agatattctc ggcacctttc
     1141 gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcactaat ccccaattcc
     1201 tccagcctcg tgtcaccgct aatcacgacc gactgatctc ccagctcttt acacagacgt
     1261 attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa cataacatcg
     1321 ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt aagccgctcg
     1381 ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt caagtcgcag cgggcgaccc
     1441 agtgcctgag caacgttatt caaaaattcg ctccgattat ccattctttt tctcctgcgc
     1501 ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa gatcgcgtgc
     1561 ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat tgataaacca
     1621 gcttgccgca tgagcgccgg ccatcatccc gactttccac aatcctggat gactattggc
     1681 ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatccctt tttcagccat
     1741 cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac acacgctgtc
     1801 acaagcagtg cataaagagc aggcgtaggg taaatcttta aaatctttat agccgccaag
     1861 tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc catgaccgcc
     1921 aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac agcgcagcac
     1981 atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa ccagatgaaa
     2041 ctcttcagga ccatcaacgt gccccgcttc gcgcggtcct gtcagccagg tgttgtatcc
     2101 cgtcaaacgt gcaccaacgg cactgcgcgc cagcatggtg atcaatacat ctacctcggc
     2161 aaacgtgggg gcaatacgct ccattcccat cactgcaata tgcgttttag gcagcgtggt
     2221 acacattcgc gcattacctt cattggtcac taggcatacc gaacctgtct ctgccaccgc
     2281 gaaattacag ccggtaatac ctatttcagc actgaggaaa tcttcgcgga ttttttgccg
     2341 gatgaataag gtcatcgctt caggcgtttc cggcccctca tagcccagac gttcgtgtag
     2401 cactcgacgg atctgatggc gatctttatg aattgccggg accacaacat gagatggcgg
     2461 atcttgatcc agctggagaa tatattcacc cagatcggtt tcaatcacct gaatgccagc
     2521 atcctgcaac acatgattga caccaatctc ttcggtcacc atcgatttag atttcaccac
     2581 cttccgggca tttttgcgtt gggcaacctg taaaatgtag cgggtagcgt cttctttggt
     2641 ttttgcaaac cagacgtgac cgccgttttg cgtcactttt tctgagagct ggtacagata
     2701 agcgtcgaga ttactcagaa catgatcacg tatctgggcg gcccgatcgc gccactcctc
     2761 ccagtgcccc aattcatcga ccattttttg ccgatttgcc ccaatacgct gctgcgcgtt
     2821 tgccaccgct ttgcgcatga tcggatcttc aatttgctga cggatgcgtg tcttaaaatc
     2881 tgtattactg gttttgatcg acatctttat atcctcagcg gctcatcaac acttcagcaa
     2941 tatgcatcac tttgactttc tgcccttccc gttgtaatcg cccactgatg ttcagcaggc
     3001 aactcacgtc agcaccaatt aaatactcag ggcggacttc catcaggtgc acaacctttt
     3061 ctttcaccat ctcgccggat atttcggcca ttttgaccga gaacgtgccg ccaaatccgc
     3121 agcaggtatc ctgttcagca aaggtcaaca gctccagtcc acgcacattt ttcagcagcg
     3181 taagtggctc gtccttcact cccagcttac gggccaggct acaagatggg tgatacacag
     3241 ctctcccttg caaactggca cctacatcga ctacccctaa tttattaaca ataaaagagg
     3301 tgagatcctg catacgcgcg gcaacctttt cggcacgtga tgcccattcg ggttcatccg
     3361 ccagatacgt cgggtaactt tttacggcat aggtgcaaga gccagccggt gaaataatgg
     3421 gatcgtcgtt atcctccagt gcggcgatca gatttttcat ccctggaatc gcttctttga
     3481 tataaccgct attgatcgca ggctgaccgc agcatccctg tttctccggg aaatttacgc
     3541 gacagccgag tttttccagt agcagcacgg agtctcgtgc cattcttgat ttcagggcgt
     3601 caccaataca ggtgacaaag aaattgacat tcacaactaa tactccatta cttcatgccc
     3661 atttatgccc gatggttccc ctccttccgg gggatcaatc atcaataaat atggcgttct
     3721 tttttaattg gctagcaaca taccacccct catttgtgtg acaaatgata atctttcatc
     3781 caaacttcaa atgcaataac taattttatt tctttcggca tcgctcacat tttatgggga
     3841 aatcgacgtt catttttggc ataagaaatc catttaaaaa ggctacatcc cttataccta
     3901 ttcagagcta cttattcagg ggttattgag atatacactg atggtgtaca aaaaaatcac
     3961 cgacaataat cacgtatcaa gtaatagtcg gggttttatt taattgaagc aatttagttt
     4021 gcatttttat ttaaaaactg agctagagtc catcagaaaa aaatatataa agcccaaacc
     4081 accgttggaa atagaaatag ttgaaaggct cacaactccc ggaatgcgga ttatctttgc
     4141 cctgatttct ggcggtttta ttcagggtgc aagctgtctg accctttccc gatattctcc
     4201 cggagtacaa ccaaactcgc ggacaaacgc cttgtgaaaa gatgattcac tggcatagcc
     4261 tactgactca gcgatcacca caacagggag catttcccgg gaaaacatct gggccgctat
     4321 ttgtagacgc aactttgtta atacagccag cggcgtggtt ccggaaacat cacggaaaag
     4381 ctgggcaaaa cttgcccgag acatgtgggc gatgctggcc agcgattcga cggtccaggc
     4441 gtgtcctggc atttccagca tttgctgtat taccgcacca agacgtggat gcagaagcaa
     4501 actgagaatg tttttctctg tattaacctg tgcaatccac tcacgcaccg cgagggtaaa
     4561 gaatgtagcg cagatctggc tacacagtgc atccactccc ggcatcgccg atctggattc
     4621 ctgttgtaaa aacgggatcg cctccctcag ccagttatat tccacgctgt ggttaaccgg
     4681 tgctaaaaac agcgtttccg gcaaagacgt taaaaaataa cgcgccgaat gttgcaaccg
     4741 aagagtgccg cagacaatac aggtcgattc attatcgaca tgactcaggc gatgagctga
     4801 attttgtggt agcaggacca catttcccgg gcgtaatgta aaaatctctc ccgtcggcat
     4861 ttccagcttc gccgctcctt gcgttaacgc atgccaacga ataaccgata attccccggc
     4921 accatgttga agctgccagt cacttcctaa cacgcaattc ttatcgatcg ttccttgtgg
     4981 agcgttaagc atcaacaaac ggctaagggc atccatatca gactcctgag tggtttttga
     5041 ttacaaacac caacaatctt agtaaataac taatctaata ccatcaaatg gttaatcatg
     5101 atttttgttt tgtgattctg attaagaaaa agaaaatacc agacgataag acaaaatatc
     5161 aagcgttgtg aagaaatggt atttgctctt ttgcgtctat agtcatgatg tcaaatgaac
     5221 gtgtttcgac aggaaatcat catgaataaa tatcaggcag ttattattgg ttttggcaag
     5281 gctggaaaaa cattagccgt cacgctggca aaagcaggtt ggcgagtggc tctcatcgaa
     5341 caatcaaatg caatgtatgg cgggacctgt attaatatcg gctgcatccc aaccaaaaca
     5401 ttggttcatg acgcacagca gcacacagat tttgtccgtg ccatacagcg taaaaatgaa
     5461 gtggttaatt ttttacgtaa taagaatttt cataatcttg cggatatgcc caatatcgac
     5521 gtgatcgacg gccaggcgga gtttatcaat aatcatagcc tgcgtgttca tcggcctgag
     5581 ggaaatctgg aaattcatgg cgagaaaatt tttattaata ccggtgcaca aaccgtggtt
     5641 ccgccaattc ctggaattac caccacgcct ggtgtatatg acagcaccgg attacttaat
     5701 ctaaaagaat tgcctgggca tttaggtatt ttgggcggcg gatatattgg cgttgagttc
     5761 gcctctatgt tcgctaattt tggcagcaag gtaaccattt tagaagcagc ttcgctgttt
     5821 ttgcctcggg aagatcggga tattgctgat aatatcgcga cgattttacg cgatcagggc
     5881 gtcgatatta tcctcaatgc ccatgtggag cgaatcagtc accatgaaaa tcaagtgcaa
     5941 gtgcatagcg agcacgccca actggcggtg gatgcactgt taatagcttc cggtcgtcaa
     6001 ccggctaccg cttcgttaca tccagaaaat gccggtatcg cagtaaacga gcgcggggca
     6061 attgtcgttg acaagcaatt acataccacc gcagacaata tttgggcgat gggagatgtt
     6121 accggcgggc tgcaatttac ttatatttca ctggatgatt accgcattgt gcgtgatgag
     6181 ttgctgggag aaggcagacg tagtactgat gatcggaaaa atgtgcctta ttccgtattc
     6241 atgacaccgc cactgtccag ggttggtatg acagaagaac aggccagaga gagtggtgct
     6301 gatattcagg tggtgacatt gcctgtagct gcaattccgc gtgccagagt gatgaatgat
     6361 actcgtgggg tattaaaagc gattgttgat aataaaaccc aacgtatatt aggggcatca
     6421 ctgctgtgtg ttgactccca cgagatgatc aatatagtga aaatggtgat ggatgccggg
     6481 ctgccttata gcatattacg cgatcagata tttactcatc cgtcgatgag cgaatcactc
     6541 aatgatctat tttcattagt caaataaact caaaatcaga cgccagaaca aatattctgg
     6601 cgtctcagag aaaagaatct tattaattct ctgttactct ataacttctt aatcacttca
     6661 ttgatggtat tttatatgtt taaaaaatct gttttatttg caacactatt atctggcgtt
     6721 atggaatttt ccaccaatgc agatgataaa ataattctga aacatatcag cgtctcgtca
     6781 gtatcagcat caccgacagt tctggaggat accattgctg atatagccag aaaatataat
     6841 gcttcatcct ggaaagtcac atcgatgcga attgataata attcaaccgc aacagcagta
     6901 ttgtataaat aaggatgttc acaatggaaa aatacctgca cctgttaagt cggggagata
     6961 aaattggcct gtcattgatt cgtctgagta ttgcgattgt ttttatgtgg attgggttat
     7021 taaagtttgt cccttacgag gcagacagca ttacaccatt cgtcgcaaac agtccactaa
     7081 tgtcgttctt ttatgaacac ccggaagact ataaacagta tctgactcac gaaggcgaat
     7141 acaaaccaga agcaagggca tggcaatcgg ccaataatac ctatggtttt tccaacggtc
     7201 ttggcgtcgt ggaggtgatt attgctctgc tggttttggc taatcctgtc aatcgctggt
     7261 taggtttatt gggaggactg atggcatata ccacgccgct ggtaacactc tcatttttaa
     7321 tcaccacccc ggaggcatgg gtacccgcat tgggtgacgc tcatcatggt ttcccttatt
     7381 tatccggtgc tggtcgcttg gtattgaaag atactctgat gctggcaggt gctgtcatga
     7441 taatggcaga ttcggcgcgg gaaattctta aacaacgcag taatgaatcc agttcaacgt
     7501 taaaaactga atattgataa tccgaacact gtctgttgtt cactttttca ggcgtcaggc
     7561 cccctttctg atgatgaagc ctgacgccat attaccggag atatgacaga gtgaagatca
     7621 atatcactcc cctgttaaaa gattcttgtc gtctcaggta aaatatatga ttaatatcaa
     7681 tcatgtattt ttacagcaag acaaccacga acaaattcag gattaaaatg ttttaccgac
     7741 tccccaacat aacccaaatc aagcgaacta atttgtgcca tttctttctc tgtcagtgag
     7801 aaatcccaga tagcaaaatt ttcttcgata cgctgttgat gtgtcgattt cggaataacg
     7861 accactcccc gttgaatatt ccaacgcaga atgacttgag aaatcgattt ttgatgggca
     7921 tctgcaatac tctgaagcag attattttca aagggtttat gtcgtccacc acctaacgga
     7981 gcccatgctt caggctgcac gttataatac ttcatggttg ccagcgcctc tggttgggca
     8041 aaatagggat gcaactcgac ctggttgacc atcggtttaa ctcttaccgt ttcacaaaag
     8101 ttcgccagaa catgaggata gaaattggaa accccaattg cttttaattt gccctcttca
     8161 taggcatctt cgagtgcacg ccacacactg aaataatcgc gcatagcctg gtgcaataaa
     8221 tagaggtcga agtactctag tcccgatttt tttaatgatg cttcaatacc tgctgctgcc
     8281 gtatcctgat tcaacatatc ctgcccccac aatttagacg ttataaataa ctcatctcgg
     8341 gtacataaac cttcagatat agcttcacga acggcttcac cgacggcatc ttcattaccg
     8401 tataccgctg cagtgtcgat gagtcgataa cccgtgcgga tagcgttcaa cactgactgt
     8461 ttgcacacgt ttttatcggt aacctgaaaa acaccaaatc ccatcatcgg cattttcaga
     8521 ttgttactta atacggaaaa ttccactgtc agtccctcca ttaggcatga acaatgagtc
     8581 tacgttaaaa cataacctca aattagtatg tggattttga tatcacttat gcaaaaaatt
     8641 cattaataat gtaggactga aacctctcta ttttcgggga caacgaagca gacgctacca
     8701 gtgcttttgc cttcgacctt gctgtttttg atacacttag ggccctgggc ataacgaaaa
     8761 tgtgcgatat cacgaagaat taatggagaa tg
//
LOCUS       DAZPBM010000162         8726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPBM010000162 DAZPBM010000000
VERSION     DAZPBM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.163"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHUZQ_003493"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHUZQ_003493"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR1027104.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHUZQ_003494"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHUZQ_003494"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1027105.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHUZQ_003495"
     CDS             complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHUZQ_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1027106.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHUZQ_003496"
     CDS             complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHUZQ_003496"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1027107.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2137 a         2026 c         2231 g         2332 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacagg tgattagtct ttatctgccg gacttaacgt cactgaagag
     4921 agataattca gcagggcgat atcgttctcg acacccagct tcatcatcgc agatttcttc
     4981 tggctactga tggttttaat actgcggttc agctttttag cgatctcggt caccaggaag
     5041 ccttccgcaa acaggcgcag aacttcactc tcttttggcg agagacgctt gtcaccgtaa
     5101 ccaccagcac tgattttttc caacaggcga gaaacgcttt ccggggtaaa tttcttccct
     5161 ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg caccttgttt cagcacgatc
     5221 ccttcgatat ccagatccaa taccgcacta agaatcgccg ggttgttgtt catagtcaga
     5281 acaatgatcg acaggcttgg gaaatggcgc ttgatgtact tgattaaggt aatgccatcg
     5341 ccgtacttat cgccaggcat ggagagatcg gtaatcaaca catgcgcatc cagtttcggc
     5401 aggttgttga tcagtgctgt agagtcttca aattcgccga caacattcac ccactcaatt
     5461 tgctcaagtg atttgcgaat accgaacaag actatcggat ggtcatcggc aataattacg
     5521 ttcatattgt tcatgtaata ggctaccttg ctacagcaag ctcttgacat aactgtcaat
     5581 gtcgctgatg tatttttcta ttcctggaac atccttctca cgaatcagat gttccagcgt
     5641 ttcacataac tgcttgccgg gtaccagatt tagcatggca aatacgcctt taagacgatg
     5701 agccgtttgg gctaacgcag caaagtcact ggttgctgct tcagtataca gcctcttaac
     5761 atcatccggt actgtgtcta caaagagcgc ataatagccg ctggcatgga gttgcgcatt
     5821 ttcatctccg cccagaggcg attctgtcac ctcttcctgc gccagttgca cttcaattaa
     5881 ttgtaagacc gcttcctgca tagcgttgct catattgaag ttgacgcaca attgaccagg
     5941 cccaatttcc cgtacgccag actcatcatc gcttaaaagc aagccagagg cagtaagatt
     6001 agacggatta tccgttaaaa agatatcata atcttgacta attaatcttt catcgggtgt
     6061 gatacaggtt gcaccccaat tttctaactg gcgagtgaca atattccgaa tttctgccga
     6121 agtaacatcc accattacgc agacatcatc cagtaaacgc tcttcttcct cttcaacttc
     6181 cgggccagcg gcgagcattt tgatatgcac agagtagcgt gtaccaagcc catcccgcgt
     6241 tttgatgttt aaatgaccgc ccagtttacg tgccagttga tcgctcagcc agaatgccag
     6301 cgggtccgcc tttccataac gatcgttttg ggtctggttg ataaacggga agtgcaaatt
     6361 atccatttca tgaatactta cgccttctcc ggtgtccaga atgcggaatg tcaggcggtc
     6421 ttcagaggac tcatcctgat caacctcaag ggtgattttt cccaattgcg ttgaggtcac
     6481 ggcatattgc atcagtagca gcaaaatacg tcgtaaggca tcgcgatcgc cgcggcgcat
     6541 atcgtgtgct ttcagatgat tgttaatcag cagttgcaga cctttacgct tgatggcagg
     6601 caacactgaa ggcacaactt catcaattaa atcctgcacg gagaacagca ccgtctcact
     6661 tttccagcta tcgtccgcaa gcatgttcgc taactgtatt tcatcgacca gccgcaccag
     6721 cacatctgcc tgattcgcca gttgtttgct ttccggggcg ttgagtttag ccgcgctctc
     6781 cgccagggac tgtgcaggtt ctttcagcgc atcgccaatg tttttcataa aggtcatccg
     6841 gccctgctgg tttttctcat acagacgctg cgcctgcttg agtttcttgt ttaccagcac
     6901 ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc ggcgcgacct ggctgcggaa
     6961 catgcggatc tcatacagct cgttattgat cgtcgcctga ataatcccct gatgctgttc
     7021 cgccatggtg gtgatgtttt gcagattcaa atgcggcagc aaatgatcgg caattttgtt
     7081 acttatgaca gtgcggttcg attcctgatc gtgaaccagc aggccgagcg gcagcagtga
     7141 gactatctct tcattgattg cccgtaaaat gcgcaattcg ttattgaccg ccgtgttggg
     7201 tagactttct gtactgcgac tggagaaatg gcggaatgtg gtatagccaa ataacgccag
     7261 cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg ttttgcaacg tatccagcaa
     7321 taaggtgcca taaggaacct gccagaccag gcgcatatcg gtagagttga gtgccgagga
     7381 gatttcaatc ttcgtactgt taaagtggat actgacgcta tccgttcctt ctttctcatt
     7441 atcattgtct cccgttgccg tcgtgtctgg ctcaaggcgg aaactgtcca gcggcatacc
     7501 cggtgggatc aaatcattaa tcggcagatc aaaagccacg accgttgcca gatgtcccgg
     7561 ctggttgaac gtggtacgca aggtaaagta atgaccgttc tgccaggcca ggcggcgcat
     7621 gttagaaaaa ctttcgcgtt catcgagggc gttggcctgt tgcaacattt ctgcacgacg
     7681 tgaatcaaca atgtcactga cggtcgattc tttaaatccg gaggtgagat ctttgagggg
     7741 cagggttgag atcagtacca gactgttatc ctgaccattc aggtaataca tcgaccacgg
     7801 tacattttct gcaccccaca atgtatccag ataagtggac atccgctgag tcatctcaag
     7861 cgttgagttg tcgtgagagc caaagattaa cgcttccgtt ttgcggcgtg gtttctccag
     7921 atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct tcgccggagg agggggaggt
     7981 cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa cgccaggtat cgacgcgctt
     8041 ttggattgcg tgggtaatgt cgacaatctg gtaactttta tctttcagcc aggcgttaac
     8101 ggcgctttgt accattaccc ccatcgtcac cagtaacaca atgatcaaca gtaaaaagaa
     8161 gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg gccgttgtct ctttctgacg
     8221 cattcgtgtt tatgacctgt taaaacttcg cgaagtgata gggcagtata aagggtacag
     8281 tgtgaaattc cagactctta cctgattgat gattctctat tctcccgtga tatttatcat
     8341 cctgcattgg gggaaataga tgtacaagcg ccattttgct gtacaaataa cctacaaaaa
     8401 gcccaacgaa attaattgca aataaacgaa gattgctgga aattatgctg atgttaatta
     8461 tttgtgaaat agttgacaag agttatagtt tttctgtggt agcacagaat aatgaaagtg
     8521 tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg ttgaaatagg agtaaacaga
     8581 cattcagaaa tgaatgacgg taataaataa agttaatgat gatagcggga gatattctag
     8641 ttgcgagtga aggttttgtt ttgacattca gtgctgtcaa atacttaaga ataagttatt
     8701 gattttaacc ttgaattatt attgct
//
LOCUS       DAZPBM010000163         8696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPBM010000163 DAZPBM010000000
VERSION     DAZPBM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.164"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHUZQ_003497"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHUZQ_003497"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1027108.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHUZQ_003498"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHUZQ_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR1027109.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHUZQ_003499"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHUZQ_003499"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR1027110.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHUZQ_003500"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHUZQ_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR1027111.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHUZQ_003501"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHUZQ_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR1027112.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHUZQ_003502"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHUZQ_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR1027113.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHUZQ_003503"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHUZQ_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR1027114.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHUZQ_003504"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHUZQ_003504"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1027115.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHUZQ_003505"
     CDS             6614..7768
                     /locus_tag="ACHUZQ_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR1027116.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHUZQ_003506"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHUZQ_003506"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR1027117.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2314 g         2052 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttct gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtg
//
LOCUS       DAZPBM010000164         8633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPBM010000164 DAZPBM010000000
VERSION     DAZPBM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.165"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUZQ_003507"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUZQ_003507"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1027118.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUZQ_003508"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUZQ_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1027119.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUZQ_003509"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUZQ_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1027120.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUZQ_003510"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUZQ_003510"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1027121.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUZQ_003511"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUZQ_003511"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1027122.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUZQ_003512"
     CDS             complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUZQ_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1027123.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUZQ_003513"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUZQ_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1027124.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2247 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa tagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAZPBM010000165         8597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPBM010000165 DAZPBM010000000
VERSION     DAZPBM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.166"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(176..1840)
                     /gene="glnS"
                     /locus_tag="ACHUZQ_003514"
     CDS             complement(176..1840)
                     /gene="glnS"
                     /locus_tag="ACHUZQ_003514"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1027125.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2099..4045)
                     /gene="nagE"
                     /locus_tag="ACHUZQ_003515"
     CDS             complement(2099..4045)
                     /gene="nagE"
                     /locus_tag="ACHUZQ_003515"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1027126.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4378..5178
                     /gene="nagB"
                     /locus_tag="ACHUZQ_003516"
     CDS             4378..5178
                     /gene="nagB"
                     /locus_tag="ACHUZQ_003516"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1027127.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5238..6386
                     /gene="nagA"
                     /locus_tag="ACHUZQ_003517"
     CDS             5238..6386
                     /gene="nagA"
                     /locus_tag="ACHUZQ_003517"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1027128.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6395..7615
                     /gene="nagC"
                     /locus_tag="ACHUZQ_003518"
                     /gene_synonym="nagR"
     CDS             6395..7615
                     /gene="nagC"
                     /locus_tag="ACHUZQ_003518"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1027129.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7663..8415
                     /gene="nagD"
                     /locus_tag="ACHUZQ_003519"
     CDS             7663..8415
                     /gene="nagD"
                     /locus_tag="ACHUZQ_003519"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR1027130.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8466..8597)
                     /locus_tag="ACHUZQ_003520"
                     /pseudo
     CDS             complement(8466..8597)
                     /locus_tag="ACHUZQ_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2101 a         2269 c         2193 g         2034 t
ORIGIN      
        1 cggatgcggc gtgaacgcct taaccggcct acaaaagcac gcaaactcaa tatattgcag
       61 agatcatgta ggcctgataa gcgtagtgca tcaggcaatt tagcgtttga aactgtttgt
      121 ctgttccttg ccggatgcga cgtaaacgcc ccatccggca tagcgaaact taaaattact
      181 cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc ggtttttccg
      241 ccgtagaatg gcggctatcg aggcagaagt aaccttcacg ctcaaactgg aatgctttac
      301 ccgcaaccgc atctttcagc gacggttcag caaagccctg tttgatcacc agcgattccg
      361 ggttaatcac cgacaggaaa tcatccgcag caccagggtt cggcacgctg aacagacggt
      421 cgtacagacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga ataacacctt
      481 tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa gtacagaaga
      541 tggtggtgat attgccttcg gcatctttct ccacgcgttc tgccttaatg acgtaagcat
      601 tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta gcttcttcgc
      661 ggaaatcggc gcgatcgatc caaatctcac cgctaaacgg cacctggcgg ctacccattt
      721 ccggtttgtt cggatggttc ggcatggtaa ccatttcgcc ttcgccctga tagttttcga
      781 taaccagttt caccggatcg ataaccgcca ttgcgcgcgg ggcgttttcg ttgagatctt
      841 cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg gtcacgccga
      901 tgcgtttgca gaactcacga atagaagcag cagtgtaacc acgacgacgc agaccggaaa
      961 tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc agcaggttca
     1021 gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg tactggcgcg
     1081 ggtgaacagg aatggtgatg ttgtccagca cccagtcgta caggcgacgg ttgtcctgga
     1141 actcaagcgt acacagagag tgcgtaatac cttccagggc atcgctgatg cagtgggtga
     1201 agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca gcaaatttaa
     1261 tgcggtacag caccggatcg cgcatcacga taaacggtga agccatgtcg attttcgcac
     1321 gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac agcgccaggt
     1381 tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggttgcgtc agggtgccgc
     1441 ggtattcgcg gatctgttcc ggcgtcagtt catcaacgta cgccaggcct ttattgatca
     1501 gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg acgttaccag
     1561 accagtgaaa acctaaccac tctacgtcgt ttttgatcga ctcaacatac tcgatatctt
     1621 cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag tcctgggcga
     1681 tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca ttcggctccg
     1741 gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca tcgatgatct
     1801 gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc tcaaagcgta
     1861 aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg ttagttagcg
     1921 aaaatcgaaa atctcactga gattatgggg atgattgatg aaaaaaaaag cggcagagaa
     1981 gatctccacc gcttttgcca gtcccgcgtc attgttggat gcgcgtctta tccaaccagg
     2041 actgagcttt tgtaggcctg ataagacgcg tcaagcgtcg catcaggcat aaagcagatt
     2101 actttttgat ttcatacagc ggtgtttgac ccgccacaac attgccctga gctttaatga
     2161 tcaagccact gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gaacgggcgt
     2221 tagcgttcag gtaatccaga tccatttcca gaatcggttg ccctgcgctc acctgcgcgc
     2281 cctcttccac cagacgttta aagcctttac cttccagcgc tacggtgtcg atacccatgt
     2341 ggacgacaat ctccgcgcct ttttcggttt ctaggcagaa cgcgtggttg gtgttgaaga
     2401 ttttcacgat tgtccctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca
     2461 caccgtcacc caccgctttg ctggcgaatg cttcgtcaga aacctgatcc agtgccacga
     2521 catcaccggt aatcggcgat accagctccg cgatagatac cgcgtttggt acagcctgcg
     2581 gttttgctac aggagcggca gttgccggag ttgcttcagc tgacgcagct gctaccgggc
     2641 cacgggcaac gactttcttc atcgcatcgc caatggattc tgctttcgcg ccaacaatca
     2701 cctgaatggt ctgtttgttc agttttacta caccagaagc acccagacgt ttacacatcg
     2761 catcgttgac gcgagctgag tcagccacgg taaggcgcag acgggtaata caggcgtcaa
     2821 tcgctttcag gttgtcagta ccgccaaccg cagcaatata gttggttgcc agttgagtca
     2881 gaccttcttc agtattgctg ttagcctctt cagttacgat ctcgtcttct ttatcttcac
     2941 gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag
     3001 cgaagaagac aacacccatc accagcagca tccagacgtt ctggctggcg gccggcaggt
     3061 tatacatcaa cgcgtagtcg atagcccccg cagagaaaga gaagccagca tggataccca
     3121 gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg
     3181 gagcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa
     3241 cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt gctgcgaagt
     3301 acatcgccag cgccgcaccc ggcagaccga acatcatgat cgggaagaag ccggacatga
     3361 acatccccgc ggtgccgtca ccagcgtaga aacggttgat gtcgccgtgg aaaaccgtac
     3421 ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc acctgatgca
     3481 gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc agcgcgcctg
     3541 cagaaacgat ccactcgccg cctgcatgga tagcgtgctg tactggcggc cagacgtaac
     3601 caaaaatggc cgccagcacc aggcagaaga agccggtggc aatcggcaca aagcgtttgc
     3661 cgccgaagaa gctcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcgc
     3721 caccaaccag accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg
     3781 tcaccatcgc tttggttaac acaaagtaac ctaccgcacc cgccagcgcc gccgcacctg
     3841 cgctgtcttt cgaccagctg gatgccacac cgatggcgaa gattaatgcg aggttatcaa
     3901 aaatcgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc
     3961 gcagcaacag tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc
     4021 gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt
     4081 taaagccgta tttttatttt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt
     4141 agatcatctg ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgcgtgcct
     4201 tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc taaagcagaa
     4261 aacttatttt atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt
     4321 tacgcttaaa gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg
     4381 agactgatcc ccctaactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc
     4441 aatcgtatca atgcgttcaa accgactgcc gatcgtccgt ttgttctggg cctgccgact
     4501 ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc
     4561 agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat
     4621 ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca
     4681 gaaaacatca accttctcaa cggcaacgcc ccggatatcg acgccgaatg ccgccagtat
     4741 gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac
     4801 ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc
     4861 ctgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt caatcaggtg
     4921 ccaaaatatg ccctgactgt cggtgttggt acattgctgg atgccgaaga agtgatgatt
     4981 ctggtgctgg gtagccagaa agcactggca ctgcaggctg cagttgaagg ttgcgtgaac
     5041 cacatgtgga ccatcagctg tctgcaactg catccaaaag cgatcatggt gtgcgatgaa
     5101 ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa
     5161 aatatcaaag gtctgtaatt gttatccctg ccctggctcc ttgctcaggg caatattttt
     5221 taaaatcggg gggcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat
     5281 ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag
     5341 cggaactgcc gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt
     5401 ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg
     5461 tcagcgtgga aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact
     5521 atctgccgac gcttatcacc accagcgatg agctgatgaa acagggcgtg cgcgttatgc
     5581 gcgagtacct ggcaagacat ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc
     5641 tgaatctggt aaaaaaaggc acccataatc cgaattttgt gcgtaagcct gatgccgcgc
     5701 tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg gcaccggaaa
     5761 tggttcctgc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgccggtc
     5821 actccaacgc gacgttgaaa gaagcgaaag ccggtttccg cgcggggatt acctttgcca
     5881 cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga
     5941 tcctcgacga agctgacatt tattgcggta ttatcgctga tggcctgcat gttgattacg
     6001 ccaacattcg taacgctaaa cgccttaaag gcgacaaact gtgtctggtt accgacgcca
     6061 ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc
     6121 gtaacggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg
     6181 aaggcgtgcg taatctggtc gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg
     6241 cgacgctcta tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag
     6301 gtaaagtagc caacctgacc tcattcacac ctgattttaa aatcaccaag accatcgtta
     6361 acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat
     6421 aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca
     6481 gtacgggcca atctcgcgga ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt
     6541 aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc
     6601 ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt tccacgcaat
     6661 cggcgtacgg cttggtcgtc acgacgccac catcaccctg tttgatctca gcagcaaagt
     6721 gctggcagaa gaacattacc cgctgccgga acgtacccag caaacgctgg aacatgccct
     6781 gttgaatgcc attgctcagt ttattgatag ctaccagcgc aaactgcgcg agctgatcgc
     6841 gatttcggtg atcctgccag ggcttgttga cccggacagc ggcaaaattc attacatgcc
     6901 gcatattcag gtagaaaact gggggctggt agaagctctg gaagaacgtt ttaaagtgac
     6961 ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg gagcactact tcggtgcaag
     7021 tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat
     7081 ctctaacggg cgcattttta ttggccgcaa cggtaacgtc ggtgaaattg gccatattca
     7141 ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc
     7201 cgccaacgct gccattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg
     7261 cgtgccgctg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag
     7321 tctggcgtcg gaagtaattg agtatgtcgg tcgtcatctg ggtaaaacca tcgccattgc
     7381 tatcaactta tttaatccgc aaaaaattgt tattgccggt gaaatcaccg aagccgataa
     7441 agtgctgctc cctgctattg aaagctgcat taatacccag gcgctgaagg cgtttcgtac
     7501 taatctgccg gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg cttttgcgct
     7561 ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc
     7621 ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta
     7681 atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa
     7741 tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg
     7801 cagaccgggc aagatctggc gaaccgcttt gccactgccg gtgtcgatgt gccggatagc
     7861 gtgttttata cctctgcgat ggcgaccgcc gatttcctgc gtcgccagga aggcaagaaa
     7921 gcatatgtgg tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt
     7981 accgatgtga accctgattt cgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg
     8041 atgcataaag cggcctattt cgtcgctaac ggtgcacgct ttatcgccac caacccggac
     8101 acccacgggc gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat tgagaaaatc
     8161 tccgggcgca aaccgttcta tgttggtaag cccagcccgt ggatcatccg cgcagcatta
     8221 aacaaaatgc aggcgcattc ggaagaaacg gtgattgtcg gcgataactt gcgcaccgac
     8281 attctggcgg gcttccaggc aggtctggaa acgattctgg tgctttctgg tgtttcatcg
     8341 ctcgacgata tcgacagtat gccgttccgc ccaagctgga tttacccgtc agtcgctgaa
     8401 atcgacgtta tctgaatacc acccacgtct gcggacgtgg gtggctctga tgacaaacgc
     8461 caaactgcct gatgcgctac gcttatcagg cctacgcagc tcctgcaata tattgaattt
     8521 tcatgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac aaatcgcact
     8581 ttatcagcaa tttggcc
//
LOCUS       DAZPBM010000166         8560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPBM010000166 DAZPBM010000000
VERSION     DAZPBM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.167"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            94..1737
                     /gene="eptA"
                     /locus_tag="ACHUZQ_003521"
     CDS             94..1737
                     /gene="eptA"
                     /locus_tag="ACHUZQ_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1027131.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            1734..2402
                     /gene="basR"
                     /locus_tag="ACHUZQ_003522"
     CDS             1734..2402
                     /gene="basR"
                     /locus_tag="ACHUZQ_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1027132.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2403..3503
                     /gene="pmrB"
                     /locus_tag="ACHUZQ_003523"
     CDS             2403..3503
                     /gene="pmrB"
                     /locus_tag="ACHUZQ_003523"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1027133.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(3479..3568)
                     /gene="pmrR"
                     /locus_tag="ACHUZQ_003524"
     CDS             complement(3479..3568)
                     /gene="pmrR"
                     /locus_tag="ACHUZQ_003524"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1027134.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(3680..5182)
                     /gene="proP"
                     /locus_tag="ACHUZQ_003525"
     CDS             complement(3680..5182)
                     /gene="proP"
                     /locus_tag="ACHUZQ_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1027135.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5446..6312)
                     /locus_tag="ACHUZQ_003526"
     CDS             complement(5446..6312)
                     /locus_tag="ACHUZQ_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1027136.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(6309..8537)
                     /gene="crfC"
                     /locus_tag="ACHUZQ_003527"
     CDS             complement(6309..8537)
                     /gene="crfC"
                     /locus_tag="ACHUZQ_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1027137.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2184 a         2295 c         2114 g         1967 t
ORIGIN      
        1 tccagcaaac ataaaagcca accttaagaa cttaaggttg gcttaatttt gctttgcgag
       61 catatgcgca ctttgttcga tggaaacacc gtgatgttga agcgcctact aaaaagcccc
      121 tctttgaatt tactcgcctg gctattgttg gccgcttttt atatctctat ctgcctgaat
      181 attgcctttt ttaaacaggt gttgcaggcg ctgccgctgg attcgctgca taacgtactg
      241 gttttcttgt cgatgccggt cgtcgctttc agcgtgatta atattgtcct gacactaagc
      301 tctttcttat ggcttaatcg accactggcc tgcctgttta ttctggttgg cgcggctgca
      361 caatatttca taatgactta cggcatcgtc atcgaccgct cgatgattgc caatattatt
      421 gataccactc cagcagaaag ttatgcgctg atgacaccgc aaatgttatt aacgctggga
      481 ttcagcggcg tgcttgctgc gctgattgcc tgctggctaa aaatcaaacc tgccacctcg
      541 cgtctgcgca gtgttctttt ccgtggagcc aatattctgg tttctgtact gctgattttg
      601 ctggtcgccg cactgtttta taaagactac gcctcgttgt tccgcaataa caaagagctg
      661 gtgaaatcct taagcccctc taacagcatt gttgccagct ggtcatggta ctcccatcag
      721 cgactggcaa atctaccgct ggtgcgaatt ggtgaagacg cccaccgcaa cccgttaatg
      781 cagaacgaaa aacgtaaaaa tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag
      841 aacttctccc tcaacggcta cccgcgtgaa actaacccgc ggctggcgaa agataacgtg
      901 gtctatttcc ctaataccgc atcttgcggc acggcaacgg cagtttcagt accgtgcatg
      961 ttctcggata tgccgcgtga gcattacaaa gaagagctgg cacagcacca ggaaggcgtg
     1021 ctggatatca ttcagcaagc gggcatcaac gtgctgtgga atgacaacga tggcggctgt
     1081 aaaggtgcct gcgaccgcgt gcctcaccag aacgtcaccg cgctgaatct gcctggtcag
     1141 tgcatcaacg gcgaatgcta tgacgaagtg ctgttccacg ggctggaaga gtacatcaat
     1201 aacctgcagg gtgatggcgt gattgtctta cacaccatcg gcagccacgg tccgacctat
     1261 tacaaccgct atccgccgca gttcaggaaa tttaccccaa cctgcgacac caatgagatc
     1321 cagacctgta ccaaagagca actggtgaac acttacgaca acacgctggt ttacgtcgac
     1381 tatattgttg ataaagcgat taatctgctg aaagaacatc aggataaatt taccaccagc
     1441 ctggtttatc tttctgacca cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt
     1501 ctgccttatg ccatcgcccc ggatagccaa aaacaggtgc cgatgctgct gtggctgtcg
     1561 gaggattatc aaaaacggta tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg
     1621 caacactatt cacaagacaa tttattctcc acgctattgg gattaactgg cgttgagacg
     1681 aagtattacc aggctgcgga tgatgttctg caaacttgca ggagagtgag tgaatgaaaa
     1741 ttctgattgt tgaagacgat acgctgttat tgcagggact gattctggcg gcgcaaaccg
     1801 aaggctacac gtgcgatggc gtaacaaccg cgcggatggc ggaacaaagc cttgaggccg
     1861 gtcattacag cctggtggta ctggatttag ggttacccga cgaagatgga ctgcattttc
     1921 tcgcccgtat ccggcagaaa aaatacaccc tgccggtact gatcctcacc gctcgcgata
     1981 cgctgaccga caaaatcgcc gggctggatg tcggtgccga tgactatctg gtgaagcctt
     2041 ttgcgctgga agagttacac gcccgtatcc gcgccctgct acgacgccat aataatcagg
     2101 gcgaaagtga gctgattgtt ggcaatctga cgctgaatat gggtcgccgt caggtatgga
     2161 tgggcggtga agagttgatt ctgacgccca aagaatatgc tctgctgtca cggttaatgc
     2221 tcaaagcagg cagtccggta catcgggaaa ttctctacaa cgacatctat aactgggaca
     2281 atgaaccctc gaccaacacc ctggaagtgc atatccacaa tctgcgcgac aaagtgggca
     2341 aagcccgtat ccgcaccgtg cgcggctttg gctatatgct ggtcgcgaat gaggaaaact
     2401 aattgaatct gatgcgtttt ctgcgccgac caatatcgct gcgccaacgg ctgatattga
     2461 ccatcggggc cattttgttg gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa
     2521 gtaccgagca gattcagctg tttgagcagg cgctacgcga caatcgcaac aacgatcgcc
     2581 acatcatgcg tgagatccgc gaggcggtcg ccagcctgat tgtccccggc gtctttatgg
     2641 tcagcctgac gctatttatc tgctatcagg cggtacgccg catcacccgc ccgctggcgg
     2701 agctgcaaaa agagctggaa gcgcgcaccg ccgacaactt aacgcccatt gccattcaca
     2761 gcgccaccct cgaaatcgaa gcggtggttt cggcgttgaa cgatctggtc agtcgcctga
     2821 cgagcacgct ggataacgaa aggttattta ccgctgacgt cgcgcacgaa ctgcgaacgc
     2881 cactggcggg ggtgcgtttg catctggaac tgctggcgaa aacgcatcac attgatgtag
     2941 caccgttagt ggcacggctt gatcagatga tggagagcgt ctcccagctg ctgcaactgg
     3001 cgcgtgccgg acagtcattt tcttccggta attatcaaca tgtaaaactg ctggaagatg
     3061 tcattctccc ctcgtatgac gaactcagta ccatgctcga ccagcgacag caaaccctgc
     3121 tattgccaga gagcgccgcc gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt
     3181 tattgcgaaa cctggtagaa aacgcccatc gttacagccc acaaggcagc aacattatga
     3241 ttaagctgca agaagatggc ggagcggtca tggccgttga agatgaagga ccgggtattg
     3301 atgagagtaa atgcggggag ttgagtaaag cgtttgtacg tatggacagc cgttatggcg
     3361 ggattggtct ggggttaagt attgtcagcc gcattacaca gttgcatcac gggcagtttt
     3421 tcctgcaaaa ccggcaagag acttccggca cgcgggcctg ggtacggctg aagaaagatc
     3481 agtacgtagc aaaccagata taaagaaagc tgctgaccac cagcacactg aacacggtag
     3541 ttaaactttc ataaacacgg tttttcattg cactctcctc cgttaacctg gaggagagta
     3601 tgcgcgtcag agattaagtg aaccttaaga gttcaattgg cctgataaga cagcgtcaca
     3661 tcaggccatc cgtttcagct tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac
     3721 gtttcgcctg caaatcggca atctcgtggt caatatcatc gattttctgc tcgatattat
     3781 cgtaatgctc gacgagaatt tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac
     3841 ctttcaacgg acgatttgcc gtctctttca tggttacgcc ggtgattaaa ccaatcaccg
     3901 ccactaccat caggtaatag gcaggcatca tcagattctg cgagctttcg accagccagg
     3961 ccgccagtgt tggcgtcaga ccggcaacca gcaccgaaat attaaatgcc gccgccagcg
     4021 cgctgtaacg gatatgcgtc gggaacatcg ctggcaaggt agaagccata acgcccgtaa
     4081 agcagttaag gatcaccgcc agcatcagta acccggcaaa aatcaggccg atgacgttac
     4141 tgttaatcag aataaacgcc gggatcgcca acacaaacag ggcaacacta ccaagtagca
     4201 caaacggacg acggccaaaa cggtcactca gcaagcccat caccggctgg acaaacagca
     4261 taccgatcat aatggcgata ataatcagca ccccgtggtc ttcggagtaa tgcaggttat
     4321 gcgacaaata actcggcata taggtcagca acatgtagta agtcacgttg gtggcaatta
     4381 ccagaccaat acatgtcaac aggctgcgcc agtatttagt ggcaatctct ttaaacgaga
     4441 ctttcgggcc atcctgcaaa ccttcgcggt cgccctgttc cagtttatca acatgctgct
     4501 ggaacgccgg agtttcttcc agcgcatggc gcaggtaaag cccgataatc cctaacggca
     4561 gagcaataaa gaacggaata cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg
     4621 tcgaaattaa caccaccacg cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca
     4681 gccagctgcc cataaagcca cgtttacggt caggggagta ttccgcaaca aatatcgacg
     4741 ccccggtata ttcaccgccg accgagaaac cttgtgccat cttacagatc aacagcagaa
     4801 tcggtgccca aataccaatc gtgtcgtagg acggtattaa gccaatacag aacgtactga
     4861 tcgacataat cacaatagtg atagcgagga tcttctggcg accatattta tcgcccaaca
     4921 taccaaagaa gagcccgcca agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg
     4981 cagcaaccat ctgcacgctg gggtcagccc ccgggaaaaa aactttacct aatgcgtaag
     5041 caacaaaacc ataaacacca aaatcgaacc attccattgc attacccagt gatgctgcgg
     5101 taatggcttt acgcagttta ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt
     5161 ttactttttt ccttttcagc atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc
     5221 agaaaaatca gcgcgacggc cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta
     5281 acaagcgtag cgggtttgct tacaccctcc ggttaaggaa tgtacaatca aaccagttga
     5341 cgcctgaatt gggcagttaa tgaccttttt accctaccag tgtttaaatt tgtgatcaac
     5401 ttcacacatg gatttcgttt aggactcatt gatgtaactg attatttacg ccaaatttac
     5461 agagaagatt tcctcgacca ttgcccccac caaacgctgc gctgaacaga gtcgtggcat
     5521 ccccagcgcg actgtttgcc agcgttgggt cactgaccag caaacgggct gtttatctgc
     5581 cgtggaccac tttcccagcc aacttaaaaa atcagcatgc tcccctcccg aagaggcaaa
     5641 ccactgatgg taatagtgac gcgtggtcgg tgccgcattg atactctctg ccagatgttg
     5701 tgtcgctttc tcgcgcaaat cacccagtaa caacaccacc gcttgcgggc ttcccagacg
     5761 gcaggcatcg ccaaatgcac cccagcgcaa ctcctccaga acgataaaac agcgcccagg
     5821 caacgaccag gcgttatacg ccccagcacg ccagcgggta aaaatttgct cgctctgatc
     5881 acctgcctgc accagtaggc ctcgttgcgt taacgccttt tccttccagc gacaacgggc
     5941 tgaaaaccac tcatacaagg cggcaatttg ctgggggagc tccgggaaag cagagtgctg
     6001 gcggtgatcc attttttgca gcagcgccag atgctgcgcg atctgcgtca gcgccagatg
     6061 accgggatcc agcatcgacg ccagtttttc caccattgtc agtcgcaggc taagcggcgc
     6121 atttaacatg ccgccgttcg tccatgtctg aagctgcaca cgcgaaagaa gttcctgcat
     6181 cagtcgttca cgaaactgct gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc
     6241 cagatccact aaaaagcggg gataaaccga ctccagcacg cgaccggggc cgtccagtaa
     6301 cgtcttggtc aatatcgttc tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg
     6361 gcgtcggtgt aaatccagcc gtgagtcatc acagtctgct gcaattgctg ctgcatcagc
     6421 ctgaccaccg attcattttg ttgacgcaga gccaggcttt cgcgtaaacg cgtctgtaat
     6481 tccgtcaaac atgaagcgaa ctcagcgcaa aaagtattca tgcctgccgt aacagattca
     6541 tcgacctgct ctgccagaac tttagccatt tgttggcaat aaagatcgac ttctgcgctt
     6601 aaagctcgtt gcaacacact gtaatcaacc gtctctgtcg gggatttctc atttccccgt
     6661 ccccagtcgg gctgattcaa ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg
     6721 ctctgctcgt ccgttgcatc ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg
     6781 acgtggggaa ctactccgtg aaaaacagga aaatgaaacc caggacgaaa ccctgactcg
     6841 ctcaattcca ttttgacttg ttgctcaatg ggccgaataa catcggttaa cacccggcaa
     6901 agggtggatt caagctcggc aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc
     6961 gtctgtaaca gtgtctcaca gcaggtacga atctcgctta acggctccga atcatcctga
     7021 aacaaggcgg ctaactgcgc attcagcgca tcttgctgtt gacgcagaaa gtggttggcg
     7081 gaggtcaggg ccagctcgat ttcatgttta atctcgccgc tcacctgcgc ctgattgagt
     7141 tgcaatagct gcaaactttc ttcgacctga tggatatttt gccgcaattg ttcacaagcg
     7201 acgtttaacc cgtgcgcacg aaaatccagg tattcccgcg cctgctgcgc gtaattcaac
     7261 agtttatgcg cagcagatcg cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc
     7321 aacgcctgaa ttggctgggc gaacagcgaa tcttcccaca actgatcggc agcatgacga
     7381 atatgttcga ggtccgccag atcggcatga cgccagcgcc tgccgagcgc ggcatgggca
     7441 aaatcttcca cccagcgttg ttgctctggc gctggtaact taccgttgtt ggctaactca
     7501 tggcgcgctc gatttgccaa gtagccccac atcgacgaca ccggaaatat ctgctgtggc
     7561 gtaatacagc ctttcatcag cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca
     7621 ctgttacgat cctgttgatc gaacttattg accagcacat acagtggcac cgattgcccc
     7681 accgccaaaa tcgcctcacg gacctcttca tcggagatcg atttcagttg cgtataatcc
     7741 agcaccgcca gtaccgccga ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc
     7801 ggttgcccgg cttcatttgg cccgggggta tccagtaacg tcaactgacc gggataactc
     7861 tccagccccg ccagatggac aaactccact tcaatcacgg gaatatgctc aatggcggcg
     7921 taagcagaaa aaggaaaatc gacgtccagc gccttcgcca gtcgcactaa atcattcaaa
     7981 cttttcagac aatgaaaaat aggctgggca cccagataat atttttcgaa agcgacgcca
     8041 ttttcgatcc gctgcataag cgcacgcata tctttatcta tttccagcac atcggtcaga
     8101 tgcttaatat cgcaatcacg caggcgctgt tgtaattgtt gaattaaaca atcgattggc
     8161 gcgacatgtg aaaaatgcag taccggttcc ttttgcccgg gcgtatggcg aataagcgtc
     8221 ggcagcgcag tcattgggcg attacgatta ggcagaacct ccgtaccaac aatggcatta
     8281 atggtggttg atttccctgc tttcatagta ccgacaatcg ccagcaccat ttccagtcgg
     8341 gaaattttac tcaactcatt attcagcatc gcgtgacgtt cggcgatatt aggctgactc
     8401 cagggtaaag ccagttgtgg cgcgtcgtct ccgggtacag agagaggcat tttttccagt
     8461 aactgcaact gttggcgaga aagctgtaac aggcgttcag cctcctgact taactcatac
     8521 agggtctgtg tgtacataga aaattcttcc ttaaagcaaa
//
LOCUS       DAZPBM010000167         8560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPBM010000167 DAZPBM010000000
VERSION     DAZPBM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.168"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(270..1013)
                     /locus_tag="ACHUZQ_003528"
     CDS             complement(270..1013)
                     /locus_tag="ACHUZQ_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1027138.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHUZQ_003529"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHUZQ_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR1027139.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHUZQ_003530"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHUZQ_003530"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR1027140.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHUZQ_003531"
     CDS             1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHUZQ_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1027141.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="ACHUZQ_003532"
     CDS             complement(2637..3404)
                     /locus_tag="ACHUZQ_003532"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1027142.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHUZQ_003533"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHUZQ_003533"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1027143.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="ACHUZQ_003534"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="ACHUZQ_003534"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1027144.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="ACHUZQ_003535"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="ACHUZQ_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1027145.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="ACHUZQ_003536"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="ACHUZQ_003536"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1027146.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>8560)
                     /locus_tag="ACHUZQ_003537"
     CDS             complement(8354..>8560)
                     /locus_tag="ACHUZQ_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1027147.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         2063 a         2148 c         2242 g         2107 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca
//
LOCUS       DAZPBM010000168         8544 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPBM010000168 DAZPBM010000000
VERSION     DAZPBM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.169"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            157..1479
                     /gene="dcuC"
                     /locus_tag="ACHUZQ_003538"
     CDS             157..1479
                     /gene="dcuC"
                     /locus_tag="ACHUZQ_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1027148.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(1522..2019)
                     /gene="sspB"
                     /locus_tag="ACHUZQ_003539"
     CDS             complement(1522..2019)
                     /gene="sspB"
                     /locus_tag="ACHUZQ_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1027149.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2025..2663)
                     /gene="sspA"
                     /locus_tag="ACHUZQ_003540"
     CDS             complement(2025..2663)
                     /gene="sspA"
                     /locus_tag="ACHUZQ_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1027150.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3058..3450)
                     /gene="rpsI"
                     /locus_tag="ACHUZQ_003541"
     CDS             complement(3058..3450)
                     /gene="rpsI"
                     /locus_tag="ACHUZQ_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1027151.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3466..3894)
                     /gene="rplM"
                     /locus_tag="ACHUZQ_003542"
     CDS             complement(3466..3894)
                     /gene="rplM"
                     /locus_tag="ACHUZQ_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1027152.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4113..5240)
                     /gene="zapE"
                     /locus_tag="ACHUZQ_003543"
     CDS             complement(4113..5240)
                     /gene="zapE"
                     /locus_tag="ACHUZQ_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1027153.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5434..5832
                     /gene="zapG"
                     /locus_tag="ACHUZQ_003544"
     CDS             5434..5832
                     /gene="zapG"
                     /locus_tag="ACHUZQ_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1027154.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5986..7353
                     /gene="degQ"
                     /locus_tag="ACHUZQ_003545"
     CDS             5986..7353
                     /gene="degQ"
                     /locus_tag="ACHUZQ_003545"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR1027155.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            7443..8510
                     /gene="degS"
                     /locus_tag="ACHUZQ_003546"
     CDS             7443..8510
                     /gene="degS"
                     /locus_tag="ACHUZQ_003546"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1027156.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         2072 a         2164 c         2187 g         2121 t
ORIGIN      
        1 taatcataag cgcatatttt ttaatgaaaa atcacatcac ctacaattaa aaacacgaca
       61 tccgcaccat aaatagactt gcaaaaaata taacatcatt gttttcaatc tgccgtttat
      121 gggattgacc gttttctttt gacacggagt tcaacaatgt tcggcataat tatatctgtc
      181 atcgtattaa ttacgatggg ctatttgatc ctgaaaaact acaaacctca ggtggtgctg
      241 gctgccgcag gtgtcttcct gatgatgtgc ggagtctggt tagggttcgg tggtgtactc
      301 gatcccgcca aaagcagcgg ctacttgatc gtcgacattt ataatgaaat cctgcgcatg
      361 ctgtccaacc gcattgccgg attggggctg tcgattatgg cggtgggcgg ttatgcccgc
      421 tacatggagc gcataggagc cagtcgcgcg atggtgagct tgctaagccg cccgttaaaa
      481 ctcattcgct cgccgtatat tattctgtcg gcaacttacg tcatcggcca aatcatggcg
      541 cagtttatta ccagcgcctc cggtctgggt atgttgctga tggtcacctt atttccgacg
      601 ctggtgagtc tgggagtaag tcgcctctct gcggtggcgg ttatcgcaac cacgatgtcc
      661 attgagtggg ggattctgga aacgaactcc atttttgcag cccaggtcgc gggaatgaaa
      721 attgccacat acttcttcca ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg
      781 gcgatctccc actttttcgt gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac
      841 gaacaggcag agcaaaaaac tctcgataat gtcccgccgc tctattacgc cattttacct
      901 gtgatgccgt taatcctgat gctcggctcg ctgttcctcg cccacgtcgg gctgatgaag
      961 tcagaactgc atctggtggt ggtgatgtta ctaagtttga cggtgacgat gtttgttgag
     1021 ttcttccgca agcataactt gcgcgaaaca atggacgatg tgcaggcgtt ttttgacggc
     1081 atgggtacgc agtttgccaa cgtggtaacg ctggtggtcg cgggtgaaat atttgcgaaa
     1141 ggcttaacga cgattggcac tgttgatgcg gttatcaggg gtgcggagca ttctggtctg
     1201 ggcggtattg gcgtgatgat tattatggcg ctagtcattg ccatttgtgc cattgtgatg
     1261 ggctctggca atgcgccgtt tatgtcattt gccagtctta ttccgaatat cgcagccgga
     1321 ctacatgtac cagcggttgt aatgattatg ccgatgcatt ttgccacgac gctagcgcgc
     1381 gcggtttcgc cgattactgc ggtggtgaag cggacagcga tccccatggc agtcggtttc
     1441 gtggtgaata tgattgccac aatcacgcta ttttattaag tcattaaaaa gacaaaacag
     1501 gccgcctggg cctgttttgt attacttcac aacacgtaat gccggtcgac caccgcgtgg
     1561 tggctgcgga ggttcatcgt caggatgagt gtcatcatcg tgatctggct tgtcgccatc
     1621 aataaccgac ataacggttt cgttgtctgc cgatgcctct tcatcattca tgatgctggt
     1681 atcttcatcg taggcagctt caggctcaaa catcgtgcct gcgccatttt cacgggcgta
     1741 gatagccagc acggcagcca gcggcacaga aacctgacgc ggaatgccac caaagcgcgc
     1801 gttaaagcgc acctcatcat tcgccagttc cagattgccg acagcacgcg gcgcaatgtt
     1861 gagtacgatt tgcccgtcac gcgcatattc cataggaacc tgcacgccag ggagcgtcac
     1921 atccaccacc aggtgcggcg tgagctggtt atccagcaac cactcataga atgcacgcag
     1981 cagataggga cgacgtggtg ttagctgtga caaatccata cagattaact ccggcccaga
     2041 cgcatttcac gttctgcttc agttaaagaa gcaaggaaag agtcacgctc aaagacgcgg
     2101 gtcatatagc ctttcagctc tttcgcaccc gggccgctga gctcgatacc cagttgcggc
     2161 agacgccaca gcagcggagc aagatagcaa tcgaccaggc tgaactcatc gctcaggaag
     2221 tacggcttct gaccgaagac cggcgcaatc gccagcagtt cttcgcgcag ttgcttacgt
     2281 gcggcatctg cttcagaagc tgaaccgttg atgatggtgt tcatcagcgt gtaccagtct
     2341 ttttcgatgc gatgcatgta cagacggctt tcaccgcgag ctaccgggta aacaggcatc
     2401 agtggcggat gcgggaaacg ctcatccaga tattccataa tgatgcgaga ttcccacagg
     2461 gtcagctcac gatccaccag ggtcggaacg ctctgattcg ggttgaggtc aatcagatcc
     2521 tgaggcggat tgtccttttc cacgtgttcg atctcgaaac ttacaccttt ctcagccagc
     2581 acaatgcgga cctgatggct atagatgtca gtaggaccgg aaaacagcgt cattaccgaa
     2641 cgtttgttgg cagcgacagc catgaaaacc tccaggtata gtcagaattt ttactgctac
     2701 cagccaccag gtggccagtc agaagttgtg ttacccaata aggaacgact ctctttgttc
     2761 gaaaatcaaa caaaaaatga gcaatacccg acatttgggc agaaaattgg atgatagttt
     2821 accagatttt gcgaccattg tggtgagtgg atgccggaaa tggggaaaaa gagatgcgct
     2881 ttagtctgaa atagttgact tagaccctta ttggcgatgt ggtttttgtt ttgcctgtct
     2941 ttcagacggc agcaaaaagc aacttcccat tttttacgct gattcagaat ttagccataa
     3001 aaaaacccgc cgaagcgggt tttttcgtaa attgttttct gccggagcag aagccaatta
     3061 acgtttggag aactgcggac gacgacgtgc tttacgcaga ccgactttct tacgttcaac
     3121 ctgacgagcg tcacgagtaa cgaagccagc tttacgcagt tcagaacgca gggactcgtc
     3181 gtattccatc agagcgcggg tgataccgtg acggatcgca ccagcctgac cagagatacc
     3241 accaccttta acggtgatgt acaggtccag tttctcaacc atgtcgacca gttccagcgg
     3301 ctgacgaact accatgcggg cagtttcacg accgaagtac tgttccagag aacgttggtt
     3361 gattacgatt ttaccgttgc ccggtttgat gaaaacgcga gctgcggaat ttttgcggcg
     3421 accagtgccg tagtattgat tttcagccat tgcctataat cccgattaga tgtcaagaac
     3481 ttgcggttgc tgtgccgcgt ggttgtgctc gttacccgcg taaactttca gtttacggaa
     3541 catagcacga cccagcgggc cttttggcaa catgccttta accgcgattt caatcacacg
     3601 ctcaggacgg cgagcaatca tctcttcaaa gctcacttgt ttgataccac cgatgtggcc
     3661 ggtgtggtga tagtacactt tgtcagtacg cttgttgccg gttacagcaa ctttgtcagc
     3721 gttcagaacg atgatgtaat caccggtatc tacgtgcgga gtgtattccg ctttgtgctt
     3781 accgcgtagg cgacgagcca gttcagtagc cagacggccc agagttttac cggtcgcgtc
     3841 aacaacatac cagtcgcgtt ttacggtttc tggtttagct gtaaaagttt tcattaaaag
     3901 cttacccaat aaatagttac acgttggtga acacccaaac gtcttcaatt gttgaggttc
     3961 acacgacaaa gtccggcaaa cctacccctt cgaatagcct atgccagcac acaaaaagtt
     4021 ttgggaaaaa aactttcttg taacgtgggg tcgcaggatt atagagaagt cggggtcaaa
     4081 gatcgacccc tttttgtgat ttgtgacagg ttttaacccg ccaaatgctc gcgcttcaga
     4141 tactcttcgc tttgcatctc ttgcagacgt gacaggcaac gctggaactc aaacttcagc
     4201 cgatcgccct gataaatttc atacagcggc acttctgcac tcaccactaa tttgacatgg
     4261 cgctcgtaaa actcatccac cagcgcaata aagcgccgcg cttcgctctc catcaaccgc
     4321 gtcataactg gtacatcaaa caacatgacc gtatgaaaga gacgtgagag cgcaatatag
     4381 tcatgctgac tgcgggcgtc gacgcacagc gtagtaaaag agaccgccag cgtctggttc
     4441 tcgacgccca ttgtcgctaa tggccgatgg ttgatttcta acgtcggtga attttctcgt
     4501 ttcccccccg ccagcgccaa ccatagttta tccatttgcg cccgggtttc atcgtggagt
     4561 ggcgaaagcc acagatgcgc ctgagtgagt gtacgcagtc gataatcaac accagcgtcc
     4621 acgttcatta catcacaatg ctgtttaatg gcatcgattg caggcagaaa acgcgcacgt
     4681 tgcaagccat ttcgataaag ttcgtccggc ggaatatttg acgtcgctac cagggtaata
     4741 ccgcgggcga acaaggcttt catcagaccg ccaagtagca tggcatcggt aatatcagaa
     4801 acaaaaaatt cgtcaaaaca gagcacgtca gtttcggctt taaagcgatc ggcaataatt
     4861 tccagcggat cggtctgccc ctgtaaggca gttagctctt catgcacccg cagcataaaa
     4921 cggtgaaagt gcaggcgctg tttccgctct cccggcaggc tttgatagaa aaggtccatc
     4981 agccaggttt tcccgcgtcc tacaccgccc cacatatata agccacgcac tggcgtatgc
     5041 tttgtgtctt cgcgtttacc ccacagctta ccgacccgcg ccattagccc actcgtcctg
     5101 ggggctggtg gcgtgctatt gatgagttcc tgataaataa tttccaggcg gctgacggcc
     5161 tctttttgaa cgtcgtcggg ttgatggctg ccttcattga gcgccttcag gtattgcgat
     5221 gttggggtaa cgctttgcat gatcttattg ttattccttg aataatcggt gcgccgttgt
     5281 tcacggttga cgaaaaaaag gccgttctac actacgcgat atgcagtcgg gattccactt
     5341 ctgtggaatt aacggttata gtggcataat cagccgcagg catggagcct gaagccaaca
     5401 ccctacggaa acaaaagaca acgggagatg ttcatgacct gggaatatgc gctaattggg
     5461 ttagtcgtcg gcatcattat tggtgctgtg gccatgcgtt ttggtaatcg taaactacgc
     5521 cagcaacagg cgttgcagta cgaactggaa aagaataaag ctgaactgga cgagtatcgc
     5581 gaagagctgg ttagccactt tgcccgcagc gcggaattac tggataccat ggcgcacgac
     5641 tatcgccagc tgtatcagca catggcgaaa agctccagca gcctgctgcc ggaactgtct
     5701 gctgaagcaa acccgttccg taatcgtctg gcagagtctg aagccagcaa cgatcaggca
     5761 ccggtgcaga tgcctcgcga ctattctgaa ggcgcatccg gcctgctgcg tactggcgcg
     5821 aagcgcgact aatttatttt tcgggcgcag ccattgcgcc ctcctcttct ctccctcccc
     5881 gactatcatt taatctggtg tctcattgtt agccgtctga aaattcaata acatcaaact
     5941 gttttgaatc tcttttctta tcattcaggt acgagagcag gaataatgaa aaaacaaccc
     6001 cagctgttga gtgcattagc gttaagtgtc gggttaactc tctcggcgtc atttcaggcc
     6061 gtcgcgtcga ttccaggcca ggttgccgat caggcccctc tccccagtct ggctccaatg
     6121 ctggaaaaag tgcttccggc agtggtgagc gtacgggtgg aaggaacggc cagtcaggga
     6181 cagaaaatcc cggaagaatt caaaaagttt tttggtgatg atttaccgga gcaacctgca
     6241 caacccttcg aaggtttagg ctccggtgtc atcatcaacg ccagtaaagg ctatgtgctg
     6301 accaacaacc atgtgattaa tcaggcacag aaaatcagta ttcagctcaa tgatgggcgc
     6361 gagtttgatg caaaactgat tggtagcgat gaccagagcg atatcgccct gttacaaatt
     6421 caaaacccga gcaaattaac gcaaatcgct attgccgact ccgataaatt gcgcgtcggt
     6481 gattttgccg tagcggtcgg taacccattt ggccttgggc aaaccgccac ctctggcatt
     6541 gtttccgcat taggacgcag cgggttgaat cttgaaggtc tggaaaactt tatccagaca
     6601 gatgcttcca ttaaccgcgg taactccggc ggtgcactgt taaaccttaa cggtgagtta
     6661 attggcatca acactgcaat ccttgcgcct ggcggcggga gcgtcgggat tggatttgcc
     6721 atccccagta atatggcgcg aacactggcg cagcagctta tcgactttgg tgaaatcaaa
     6781 cgcggtttgt taggcatcaa aggcaccgag atgagtgccg atatcgccaa agccttcaac
     6841 cttgacgtgc agcgtggcgc gtttgtcagc gaagtgttgc caggttctgg ctcggcgaaa
     6901 gcgggcgtca aagcgggcga tattattacc agcctcaacg gcaaaccgct gaatagcttt
     6961 gctgagttgc gctctcgtat cgcgaccacc gagccgggca caaaagtgaa acttggcctg
     7021 ctgcgtaacg gcaaaccact ggaagtagaa gtgacgctcg ataccagcac ctcttcgtcg
     7081 gccagcgctg aaatgatcgc gccagcgctg gaaggtgcaa cgttgagcga tggtcagcta
     7141 aaagatggcg gcaaaggtat taagatcgat gaagttgtca aaggaagccc agctgctcag
     7201 gctggcttgc aaaaagacga tgtgatcatt ggcgtcaacc gcgatcgggt gaactcgatt
     7261 gctgaaatgc gtaaagtgct ggcggcaaaa tcggccatca tcgccctgca aattgtacgc
     7321 ggcaatgaaa gcatctatct gctgatgcgt taatgtcgta aaccgggcat caggcttacg
     7381 tgtgatgtcc ggttaactca tggtatgctg ctgccgttcc cttttttaat gacgcctcca
     7441 tcatgtttgt gaagctctta cgttccgttg cgattggatt aattgtcggc gctattctgc
     7501 tggttgccat gccttcgctg cgcagcctta acccgctttc cactccgcaa tttgacagta
     7561 ccgatgagac gcctgccagc tataatctgg cggttcgccg cgccgcgcca gcggtggtta
     7621 acgtttacaa ccgtggtttg aacaccaact ctcacaacca gcttgagatc cgcaccctgg
     7681 gatccggtgt aatcatggat caacgcggtt atatcatcac caataaacac gtcatcaacg
     7741 acgccgatca gatcatcgtc gccttacagg atggtcgtgt atttgaagca ttgctggtgg
     7801 gatctgactc tctaaccgat ctggcggtac ttaaaattaa tgccactggc ggtttaccta
     7861 ccattccaat caatgcacgt cgcgtaccgc atattggcga cgtggtactg gcgatcggta
     7921 acccgtacaa cctcgggcag accattaccc aggggattat tagtgccacg ggtcgaattg
     7981 gtctgaaccc gaccgggcgg caaaacttcc tgcaaaccga tgcttccatt aaccacggta
     8041 actctggcgg cgcgctggtg aactcgctgg gcgaactgat gggcattaac acgctgtcgt
     8101 ttgataagag taacgatggc gaaacgccgg aaggtatcgg ctttgcgatt cctttccagt
     8161 tagcaaccaa aattatggat aagctgatcc gcgatggtcg cgtgatccgc ggctacattg
     8221 gcattggcgg acgtgagatc gcaccgctgc acgcacaggg cggtggtata gatcaactgc
     8281 aagggatcgt ggttaatgaa gtgtcacctg acggcccggc ggcgaatgcg ggtattcagg
     8341 tcaacgatct gattatttcg gtggataaca aaccggccat ctctgctctg gagacgatgg
     8401 atcaggtggc ggaaattcgc cctggttcgg tgatcccggt tgtagtgatg cgtgatgata
     8461 agcagttaac gctgcaggtc accattcagg aatatccggc aaccaattaa gtcgagtgct
     8521 caaaacaaaa aaccggagtc tgtg
//
LOCUS       DAZPBM010000169         8268 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPBM010000169 DAZPBM010000000
VERSION     DAZPBM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.170"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHUZQ_003547"
     CDS             <1..189
                     /locus_tag="ACHUZQ_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027157.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
     gene            412..909
                     /gene="ubiC"
                     /locus_tag="ACHUZQ_003548"
     CDS             412..909
                     /gene="ubiC"
                     /locus_tag="ACHUZQ_003548"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1027158.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            922..1794
                     /gene="ubiA"
                     /locus_tag="ACHUZQ_003549"
     CDS             922..1794
                     /gene="ubiA"
                     /locus_tag="ACHUZQ_003549"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1027159.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHUZQ_003550"
     CDS             complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHUZQ_003550"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1027160.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHUZQ_003551"
     CDS             4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHUZQ_003551"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1027161.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5021..5629
                     /gene="lexA"
                     /locus_tag="ACHUZQ_003552"
     CDS             5021..5629
                     /gene="lexA"
                     /locus_tag="ACHUZQ_003552"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1027162.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5702..7027
                     /gene="dinF"
                     /locus_tag="ACHUZQ_003553"
     CDS             5702..7027
                     /gene="dinF"
                     /locus_tag="ACHUZQ_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1027163.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7143..7352
                     /locus_tag="ACHUZQ_003554"
     CDS             7143..7352
                     /locus_tag="ACHUZQ_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1027164.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHUZQ_003555"
     CDS             complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHUZQ_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1027165.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1889 a         2004 c         2252 g         2123 t
ORIGIN      
        1 gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga
       61 cataagatgt tgcctattaa tcttactcag gaaacacttt ttagctccaa atctcatggt
      121 aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaatata
      181 ggtatttaag taaaggaaaa catcaccgtt cctggcatca tgggcggtga tgcccctacg
      241 gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc
      301 atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta
      361 aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac
      421 cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggattcg
      481 caactgctcg actggctgtt gctggaagat tccatgacaa aacgttttga acagcaggga
      541 aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aatccccgaa
      601 gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc
      661 gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg
      721 gagctggcgt tacaaaaatt gggtaaaacg ccgctaggac gctatctgtt cacatcatcg
      781 acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc
      841 cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg
      901 ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt
      961 catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta
     1021 tgggcgttgt gggtggcgac accgggcgtt ccccagctct ggatcctggc ggtgtttgtc
     1081 gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga atgattatgc tgaccgcaag
     1141 tttgatggtc atgttaagcg cacggcgaac cgaccgcttc ccagcggcgc ggtaacggag
     1201 aaagaggcgc gcgcgctgtt tgtagtgctg gtattgattt catttttact ggtgctgacg
     1261 ctgaatacga tgaccattct gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg
     1321 tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg
     1381 attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc
     1441 ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcaatggt tgaccgtgac
     1501 gatgatgtga agattggtat taaatccacc gcaatcctgt tcggccaata cgataaattg
     1561 attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat
     1621 ggcttaggct ggggatatta ctggtcaatt ctggtggctg gcgcgctgtt tgtttatcaa
     1681 caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac
     1741 tatgttggtc tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt
     1801 aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag
     1861 gcctacacaa cacatcgcaa tttattgaat ttgcagatta tggaaggccg gataaggcgt
     1921 tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg
     1981 tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg
     2041 ccggttcggc gtcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag
     2101 aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac
     2161 gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac
     2221 tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca
     2281 gctgtagggg gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca
     2341 gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat
     2401 cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca
     2461 atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca
     2521 ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgcggca
     2581 gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt
     2641 gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt
     2701 tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc
     2761 gctgacgtga tgccagaagc gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt
     2821 tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg
     2881 gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt
     2941 aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct
     3001 tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt
     3061 ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg
     3121 taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt
     3181 tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat
     3241 aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc
     3301 ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc
     3361 agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca
     3421 cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat
     3481 gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa
     3541 gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat
     3601 tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt
     3661 ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat
     3721 taaacagatc ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa
     3781 agtgcatacg cgccacgcgc gccagtttct gtgcgatagt tttatccgtg ccgtgttcat
     3841 ccgccatacg gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg cgaccgagcc
     3901 acagtacggc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg tttacttcgc
     3961 ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca
     4021 gatttgggtt gctacggtgc aaatcgagat agtcgtggaa cagcttaata gactcttctt
     4081 tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta
     4141 gcgtgccgtc gatttctagc ggctctaacg ggtcaggcaa gtcatgagcc agacactggg
     4201 cgcgtaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag
     4261 aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga
     4321 tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg
     4381 taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt
     4441 ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg
     4501 gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact
     4561 ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg
     4621 atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc
     4681 tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg
     4741 atgattgtgg aaattctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa
     4801 taccatgagc tttccggacg cgctaaagat atgggatccg ccgcggtgct gattgcgatt
     4861 atcgtcgccg tgataacctg gtgcatcctg ttatggtcgc attttggata acccttccag
     4921 atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta
     4981 tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag
     5041 gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac
     5101 gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
     5161 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg
     5221 tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc
     5281 gcttctggcg cagcagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc
     5341 gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga
     5401 tggtgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc
     5461 acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggtaata aagtcgaact
     5521 gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
     5581 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga
     5641 gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg
     5701 catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt
     5761 ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga
     5821 tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat
     5881 gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa
     5941 aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggctgg
     6001 ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg gctctgcata ttgttggcgg
     6061 tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgcacc
     6121 ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc
     6181 gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctagt
     6241 gatggggctg catatgaacg tgcagggcgc ggctctggcg acggttattg cggaatatgc
     6301 aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg
     6361 cgaaatgtcg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga
     6421 tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggcgc
     6481 gcgactgggg agtgacatta tcgctgtaaa cgcggttctg atgacgctac tcacctttac
     6541 cgcctatgcg ctggatggtt ttgcctacgc ggttgaagca cattccggtc aggcatacgg
     6601 tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca gcgtgccgcc agtcggggat
     6661 cgtagcgtta ctattttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct
     6721 gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt
     6781 gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc
     6841 cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac
     6901 gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg
     6961 cgggctttct ctggcggcta tctggcggcg tcactggcgt aacggtacct ggtttgccgc
     7021 aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa
     7081 tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga
     7141 ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc
     7201 aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg
     7261 tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg
     7321 attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag
     7381 gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact
     7441 gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa
     7501 tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca
     7561 cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcatataaac atggctgacg
     7621 tatgcgtggg ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat
     7681 gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt
     7741 gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca
     7801 gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc
     7861 agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca
     7921 aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt
     7981 attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttatgttg ccttgtcgta
     8041 ccattatcaa cacgataata attaataatt catttttaaa taaattgctt caagttaact
     8101 cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt
     8161 tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga
     8221 tgaatcatgt tgaagataat acccggtgca actggatact ttaataaa
//
LOCUS       DAZPBM010000170         8213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPBM010000170 DAZPBM010000000
VERSION     DAZPBM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.171"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="mgtT"
                     /locus_tag="ACHUZQ_003556"
     CDS             <1..100
                     /gene="mgtT"
                     /locus_tag="ACHUZQ_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR1027166.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHUZQ_003557"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHUZQ_003557"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR1027167.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1753)
                     /gene="ydeI"
                     /locus_tag="ACHUZQ_003558"
                     /pseudo
     CDS             complement(1360..1753)
                     /gene="ydeI"
                     /locus_tag="ACHUZQ_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
     gene            2029..2547
                     /locus_tag="ACHUZQ_003559"
     CDS             2029..2547
                     /locus_tag="ACHUZQ_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1027168.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2592..4637)
                     /gene="dcp"
                     /locus_tag="ACHUZQ_003560"
     CDS             complement(2592..4637)
                     /gene="dcp"
                     /locus_tag="ACHUZQ_003560"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1027169.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4774..5520
                     /gene="ydfG"
                     /locus_tag="ACHUZQ_003561"
     CDS             4774..5520
                     /gene="ydfG"
                     /locus_tag="ACHUZQ_003561"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1027170.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5609..6295
                     /gene="rspR"
                     /locus_tag="ACHUZQ_003562"
                     /gene_synonym="ydfH"
     CDS             5609..6295
                     /gene="rspR"
                     /locus_tag="ACHUZQ_003562"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1027171.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6472..6675
                     /gene="ydfZ"
                     /locus_tag="ACHUZQ_003563"
     CDS             6472..6675
                     /gene="ydfZ"
                     /locus_tag="ACHUZQ_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1027172.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6711..8171)
                     /locus_tag="ACHUZQ_003564"
     CDS             complement(6711..8171)
                     /locus_tag="ACHUZQ_003564"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1027173.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2169 a         2056 c         1871 g         2117 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcca tcctgtttta
     1681 aaccgccctg gtcatctgcc aacgcataag gcatgacaag aaaacttgct aatacaatgg
     1741 actgaaattt catactaact ccttaattgc gtttggtttg acttattaag tctggttgct
     1801 atttttataa ttgccaaata agaatactgc caattgttat aaggcattta aaatcagccc
     1861 actagctgtc aaatattcag agaatttgac tcactaaagt taagaagatt gaagagtctt
     1921 aaacatgttt tcagaataat cggatttata tgtttgaaaa ttattatatt ggacgagcat
     1981 acagaaaaag caaatcacct ttacatataa tagcgtggac aaaaaacagt gaacattaat
     2041 aaagataaaa ttgtacaact cgcagatacc gacactattg aaaacctgac atccgcgttg
     2101 agtcaaagac ttatcgcgga tcaattacgc ttaactaccg ccgaatcatg caccggcggt
     2161 aagttggcta gcgccctgtg tgcagctgaa gatacaccca aattttacgg tgcaggcttt
     2221 gttactttca ccgatcaggc aaagatgaaa atcctcagcg taagccagca gtctcttgaa
     2281 cgatattctg cggtgagtga gaaagtggca gcagaaatgg caacaggtgc catagagcgt
     2341 gcggatgctg atgtcagtat tgccattacc ggctacggcg gaccggaggg cggtgaagat
     2401 ggtacgccag cgggtaccgt ctggttcgcg tggcatatta aaggccagaa ctacactgcg
     2461 gttatgcatt ttgctggcga ctgcgaaacg gtattagctt tagcggtgag gtttgccctc
     2521 gcccagctgc tgcaattact gctataacca ggctggcctg gcgatatccc caggccagcc
     2581 attggtggtg tttatatgtt caagccacga tgttgcagca tcggcataat ctgaggagcc
     2641 ttaccgcgcc attgtcgatg caggcgttcc agatcttcgc tgttacctct ggaaaggatc
     2701 gcctcgcgaa aacgctgccc attttcacgc gttaatccgc cctgctcaac aaaccactgg
     2761 taaccatcat cggccaacat ttgcgtccac agataagcgt aataacctgc agcatatccg
     2821 ccaccaaaaa tatgggcgaa ataactgctg cgatagcgtg gcggtatagc aggaagatcc
     2881 atattttccg ccaccagcgc ccgcaattca aaatcatcga catcctgcat tgcttcgttt
     2941 tcttccaggc aatgccagcg catatcgaga agtgcggcgc taagcagttc gctcatctca
     3001 taccctttgt tgaacaggct ggcattacgc attttctgtt gcagttcgtc aggcattgct
     3061 gccccgctct gataatgccg ggcgtagcga gcgaatacct gcggatgcgt tgcccagtgt
     3121 tcgttgattt gcgacggaaa ttcgacaaaa tcacgcggcg tgttggtgcc ggaaagcgtg
     3181 gcataacgct ggcgggcaaa aaggccgtgc agcgtatgac caaattcatg gaataaggtt
     3241 attacatcat cccagagtaa caacgcaggc tcaccggcag cgggtttctg ataattgcag
     3301 acgttataaa ttaccggatg cgtttcatta agcgttgatt gctcaacaaa attgcccatc
     3361 catgcaccgc cgctttttga atcacgggcg aagaaatcac cgtaaaataa cgccagcccc
     3421 acgccattat gatcaaaaat ttcccacacc cgaacgtcag gatggtagac aggaatatca
     3481 aaacgttcga caaacttaat accgaagagc tgattcgcgg tccagaatac accttcattc
     3541 aacaccgtgt ttaattcaaa atatggcttg agctgcgctt catcaagatc aaatttctcc
     3601 cgccgtacct gttccgcata aaatgcccag tcccacggct gcgcgctaaa cccgccctgc
     3661 tgcttatcga taaccgcctg tatggaggct aattcatcgc tcgcacgttg acgcgccgct
     3721 ggaacaattt cccgcataaa gttgagtgct gcttctggcg tttttgccat ctgatcggcg
     3781 attttccatg cggcataatg aggaaaacca agcagtttcg cctgctgtgc gcgaatctct
     3841 accagacgtt gaatgatagc gcgggtatca ttgccatcat ttttttccgc tcgcgtccag
     3901 cccgcaataa acagtttttc acgcgtcgca cgatcgcgca tttcggcaag cgccggttgt
     3961 tgggtggtat tcagcagcgg aatcagccat ttgttatcca gacctttctc gcgagccgcc
     4021 tctgccgcca gcgcaatctc ttgctcactc attcctgcca gctgcgcgat atcgttcaca
     4081 accagaccgc cggatttatt tgctgccagt aatcgctggt taaactggct ggtcagggtc
     4141 gcagcttctg tattcagtac ttttaatttt gctttatcag cttgcgcaag tttggctccg
     4201 gcaaggacaa aacgttgatg aatcacctcc accaggcgga tggattcact atcaagcccc
     4261 agggattcac ggcgctgcca gacagcatct acccgcgcga ataattcacc gttcagatag
     4321 atatcattag ccagttccgc cagttcagcg gaaaactgtt catcaagacg ctgtaattca
     4381 tcattggtat gcgccgcagt catcgcaaaa aagacgctgg taacgcgggt aagtaattct
     4441 ccgctttgtt ccagtgccag aatagtattg ttgaaatcag gcgtttgcgg gttaagcgcg
     4501 atggcagcaa tttctgcccg cttttgctgc attccctcat cgaatgccgg gcgatagtga
     4561 tgattggcaa tttgatcaaa atggggagcc agatacggta gtgtgctttg cacaaggaaa
     4621 ggattcattg ttgtcatttt cttctcctga acgcgagatg ttccatagcg taggcttact
     4681 gataacggag tgcaatcttg caaaccagta ttacccgctc ttaagcatca cgtgctatgt
     4741 tattgacaca caaaagcgtt gaggaacagt gagatgatcg ttttagtaac tggagcaacg
     4801 gcaggttttg gtgaatgcat tactcgtcgt tttattcaac aagggcataa agttatcgcc
     4861 actggccgtc gccaggagcg gttgcaggag ttaaaagacg aactgggaga taatctgtat
     4921 atcgcccaac tggacgttcg caaccgcgcc gctattgaag agatgctggc atcgcttcct
     4981 gccgagtggt gcaatattga tatcctggta aataatgccg gcctggcgtt gggcatggag
     5041 cctgcgcata aagccagcgt tgaagactgg gaaacgatga ttgataccaa caacaaaggc
     5101 ctggtatata tgacgcgcgc cgtcttaccg ggtatggttg aacgtaatca tggtcatatt
     5161 attaacattg gctcaacggc aggtagctgg ccgtatgccg gtggtaacgt ttacggtgcg
     5221 acgaaagcgt ttgttcgtca gtttagcctg aatctgcgta cggacctgca tggtacggcg
     5281 gtgcgcgtca ccgacatcga accgggtctg gtgggtggca ccgagttttc caatgtccgc
     5341 tttaaaggcg atgacggtaa agcggaaaaa acctatcaaa ataccgttgc attgacgcca
     5401 gaagatgtca gcgaagccgt ctggtgggtg tcaacgctgc ctgctcacgt caatatcaat
     5461 accctggaaa tgatgccggt tacccaaagc tatgccggac tgaatgtcca ccgtcagtaa
     5521 tttttatacc cggcgtaact gccgggttat tgcttgtcac aaaaaagtgg tagactcatg
     5581 cagttaactc actcacaagc aagaacgaat gaccgtcgaa acgcaactta atcccacaca
     5641 gcctgtcaat cagcaaattt atcgtattct tcgtcgcgac attgtccatt gcctgattgc
     5701 accaggcaca ccgttgtcgg aaaaagaagt ttctgttcgt ttcaatgtgt cacgccagcc
     5761 ggttcgtgaa gcctttatta aactggcgga aaacggcctg attcaaattc gtccgcagcg
     5821 tggcagctac gtcaacaaaa tttccatggc ccaggtgcgc aatggcagtt ttatccgtca
     5881 ggccattgag tgcgcggtgg cgcgtcgggc ggcgagcatg attaccgaaa gccagtgcta
     5941 ccaactggaa caaaatcttc accagcaacg cattgccatt gagcgcaagc aactggatga
     6001 tttttttgaa cttgatgaca acttccatca actcctgacg cagattgccg actgtcaact
     6061 ggcgtgggat accattgaga acctgaaagc gaccgttgat cgcgtgcgct atatgagttt
     6121 cgaccacgtt tctccaccag aaatgctgtt acgccagcat cttgatattt tctctgccct
     6181 gcaaaaacgt gatggcgatg cggtagaacg tgcaatgacg caacatttgc aggaaatcag
     6241 cgaatccgtg cgccagatcc gccaggaaaa tagcgactgg tttagcgaag agtaattcat
     6301 ttcctctcat cccatccggg gtgagagtct tttcccccgc cttatggctc atgcatgtat
     6361 caaaaaagat gtgagcttga tcaaaaacaa aaaatatttc actcgacagg agtatttata
     6421 ttgcgcccgt tacgtgggct tcgactgtaa atcagaaagg agaaaatacc tatgacgacc
     6481 tacgatcgta accgtaacgc aatcaccact ggcagccgtg ttatggttag cggcaccggt
     6541 cacactggca agatcctgtc gattgatact gaaggtctga ccgctgagca aatccgccgc
     6601 ggaaaaaccg tagttgttga aggttgtgaa gagaaactgg cacctctgga cctgattcgt
     6661 ctcggcatga actaagcgtg tgaatgccgc cgatggcggc attgcttttt ttacttcacg
     6721 gaatatttcg ccacggtcgc tttcgcgcca tgcgctaata aagacaagta cgcttccgtc
     6781 acttttgcag taaacaaact attgtctggc aaatcatcac caaagatcgc cttaatcgcc
     6841 agcaatgact ggacgcgcgc tttcccttca gcactacttt gtacagcctt ctgaataaca
     6901 ggtaacagtg ggtcactgat ttcaatcgga tttccctgtt catcaacacc accgacataa
     6961 cgcatccaac ccgcgacgcc cagtgccagc agatcgaact tgctgtcatg cgccagatgc
     7021 cagcgaacag aatccaacat ccgctgtggc aatttctggc tgccatccat cgcaatctgc
     7081 caggttcgat gacgtaacgc cgggttgcta tagcgttcaa ttaatcggtt agcgtaatct
     7141 tgcaaatcaa cgccctgcac tttcagcgtc ggcgcttgtt cctgcaacat caagccatac
     7201 gccgcataac gataatgttc atcttccatg cagtcattaa tgtgctgata tccagcaaga
     7261 taacccaaat acgccaggaa tgaatgactg ccgttgagca tgcgcaactt catctcttca
     7321 taaggcagca catcactaac cagttcggct cccgcttttt cccattctgg acgtccggca
     7381 acaaagttat cttctattac ccactggcgg aaaggttcac aggcaacgcc cgcaggatcg
     7441 cccacaccgg taagttgttc gattttcgcc agcgtatcct ctgtcactgc gggcacaata
     7501 cggtccacca ttgttgatgg gaaagtcacg ttatcttcga tccattgtgc cagttttaca
     7561 tcaacagctt tcgcgtatga agtgacaacg tcacgcataa catgaccgtt ttctggcatg
     7621 ttgtcacatg acatgacggt aaatgcggga agtcctgccg ctttacggcg ggccagcgcc
     7681 tcaacaatca cccctgttgc tgttttcggc tggtggggat tttgcacgtc ggcagcgacc
     7741 atcgggtgat cgagcattaa ctgtccggtc gccggagagt ggaaataccc tttttcggtg
     7801 attgtcagag agacaatcgc gatttgcggt tcacacatcg cagccaacac ggtttctaag
     7861 ccatccatct gtacgtgcaa ggctttctta acgacgccaa cgacgcgagc cgtccacgca
     7921 tcggccgaca tttccgcaac ggtataaaga ttatcttgct gttgtaaatc ggcaatttgc
     7981 tgttcgccgc cgattaagtt gacctcataa tatccccagt cactgaaatg ttctgtagca
     8041 agaatatcgg catacacgcc ctgatgcgca cggtgaaatg caccaaagcc taaatgaata
     8101 attcttgggg ccaggttatt acgatcataa acagggagtg tcgcttttgc tgataacaaa
     8161 ttatttccca taacaattcc ttaaatataa atatggcaag ctatatgttt tgt
//
LOCUS       DAZPBM010000171         8185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPBM010000171 DAZPBM010000000
VERSION     DAZPBM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.172"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHUZQ_003565"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHUZQ_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1027174.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHUZQ_003566"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHUZQ_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1027175.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHUZQ_003567"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHUZQ_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1027176.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHUZQ_003568"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHUZQ_003568"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1027177.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHUZQ_003569"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHUZQ_003569"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1027178.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHUZQ_003570"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHUZQ_003570"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1027179.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHUZQ_003571"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHUZQ_003571"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1027180.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHUZQ_003572"
     CDS             5315..6439
                     /locus_tag="ACHUZQ_003572"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1027181.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHUZQ_003573"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHUZQ_003573"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1027182.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>8185
                     /gene="waaO"
                     /locus_tag="ACHUZQ_003574"
                     /gene_synonym="rfaI"
     CDS             7245..>8185
                     /gene="waaO"
                     /locus_tag="ACHUZQ_003574"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR1027183.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAK"
BASE COUNT         2101 a         1887 c         2071 g         2126 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttattgcgcc aagca
//
LOCUS       DAZPBM010000172         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPBM010000172 DAZPBM010000000
VERSION     DAZPBM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.173"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUZQ_003575"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUZQ_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR1027184.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUZQ_003576"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUZQ_003576"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1027185.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUZQ_003577"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUZQ_003577"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1027186.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHUZQ_003578"
     CDS             6679..>8182
                     /locus_tag="ACHUZQ_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR1027187.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZPBM010000173         8178 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPBM010000173 DAZPBM010000000
VERSION     DAZPBM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.174"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            90..1586
                     /gene="ravA"
                     /locus_tag="ACHUZQ_003579"
     CDS             90..1586
                     /gene="ravA"
                     /locus_tag="ACHUZQ_003579"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1027188.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1580..3031
                     /gene="viaA"
                     /locus_tag="ACHUZQ_003580"
     CDS             1580..3031
                     /gene="viaA"
                     /locus_tag="ACHUZQ_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1027189.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHUZQ_003581"
     CDS             complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHUZQ_003581"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1027190.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4180..4638
                     /gene="asnC"
                     /locus_tag="ACHUZQ_003582"
     CDS             4180..4638
                     /gene="asnC"
                     /locus_tag="ACHUZQ_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1027191.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4728..5171
                     /gene="mioC"
                     /locus_tag="ACHUZQ_003583"
     CDS             4728..5171
                     /gene="mioC"
                     /locus_tag="ACHUZQ_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1027192.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHUZQ_003584"
     CDS             5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHUZQ_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1027193.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHUZQ_003585"
     CDS             7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHUZQ_003585"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1027194.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2057 a         2092 c         2088 g         1941 t
ORIGIN      
        1 cccccctttg agactatttt agccaccagc aagaaacgtc tatactcgca atttacgcag
       61 aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta gcggaaagaa
      121 tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac gccatccgct
      181 tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggcccg ccaggtattg
      241 ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc gcgtttgaat
      301 atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct attcaggcgc
      361 taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa gcagaaattg
      421 tctttctgga tgagatctgg aaagcgggcc cggcaattct taataccttg ctcaccgcca
      481 ttaacgagcg ccagttccgc aacggtgcac acgtagaaaa aatcccgatg cgtctgctgg
      541 tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta tatgaccgca
      601 tgctgattcg tctgtggtta gataaagtgc aggataaagc gaatttccgc tccatgctga
      661 ccagtcaaca ggatgaaaac gacaatccgg ttcctgatgc cttgcaggtc acagatgaag
      721 aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat gtatttgagc
      781 tgatttttat gctgcgccag caactggata aattaccgga tgcgccttat gtctcggatc
      841 gtcgctggaa aaaagcgatc cgattattgc aggccagcgc cttttttagc ggtcgcagtg
      901 ctgttgcccc ggtagatctc attttgctga aagattgcct gtggtatgac gcgcaaagcc
      961 tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcatgcctgg caacagcaag
     1021 ggatgttgac tcgccttggc gcgattgtgc aacgtcacct gcaactccag cagcaacaaa
     1081 gcgataaaac agccttaacg gtaattcgtc tgggcggcat tttcagccgt cgtcagcagt
     1141 atcaacttcc tgttaacgtt actgcttcca ctctgactct actgctgcaa aaaccgttaa
     1201 aactgcatga tatggaagtg gttcatatct cctttgagcg tagcgcgctg gaacagtggc
     1261 tgagcaaagg tggtgaaatt cgcggtaaac tgaacggtat cggctttgcc cagaaactga
     1321 atctggaagt tgatagcgcc caacatcttg tcgttcgcga tgtaagttta caaggcagta
     1381 cgctggcact tcccggttca tcggctgaag gtctgcctgg tgaaataaaa caacaactgg
     1441 aagagcttga aagcgactgg cgcaagcaac acgctttatt cagcgaacag caaaaatgtc
     1501 tgtttatccc tggcgactgg ttaggtcgca ttgaagccag cctacaggat gtcggtgcac
     1561 agattcacca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc tggccgtcag
     1621 cgaagaggga ttgatcgaag agatgatcat cgcactgctg gcctcaccgc agctggcggt
     1681 cttctttgaa aaattcccac gcctgaaggc ggcaatcact gatgatgttc cccgctggcg
     1741 tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca ccgaagaggt
     1801 gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc agctaccaca
     1861 gatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc ggcagttggt
     1921 tgatgctaac agcacaataa cttcagcgtt acacacgctt tttctccagc gttggcgttt
     1981 aagtctgatc gtgcaagcaa cgacgttaaa tcaacagcta ttagaagaag aacgcgaaca
     2041 actgttgagt gaagttcaag aacgcatgac gctgagcgga caacttgaac cgattctcgc
     2101 agataacaat actgcagctg gtcgtctgtg ggatatgagc gccggtcagc ttaaacgtgg
     2161 cgactatcag ttgattgtga aatacggtga atttcttaac gaacagccgg aactgaaacg
     2221 cctggcagag cagctggggc gttctcggga agccaaatca ataccgcgca acgatgcgca
     2281 gatggaaacc ttccgcacca tggtgcgcga accggcgacg gttcctgagc aggttgatgg
     2341 tctgcaacaa agcgatgata ttttacgtct tctgccgcca gaactggcga cactagggat
     2401 aacggaactg gagtatgagt tttaccgtcg gctggtggaa aaacagttgc tcacctatcg
     2461 cctgcacggt gagtcgtggc gtgaaaaagt gatcgaacgt ccggtggtac ataaagatta
     2521 cgatgaacag ccgcgcgggc cgtttattgt ctgtgtggat acttccggct caatgggcgg
     2581 ctttaatgaa cagtgtgcga aagcgttctg cctggccttg atgcgcattg ctctcgctga
     2641 aaaccggcgc tgctatatta tgctattttc caccgagatc gtccgttatg agctttcagg
     2701 cccccaaggc atcgaacaag caatccgttt tttaagccag cagtttcgtg gcggcaccga
     2761 tcttgccagt tgttttcgcg ccattatgga acgcttgcaa agcagggaat ggtttgatgc
     2821 cgatgcggtg gtgatttctg attttatcgc tcagcggttg cctgacgacg tgacgagtaa
     2881 agtgaaagag ctgcagcggg tacatcagca tcgctttcat gccgtggcga tgtcggcaca
     2941 cggcaaaccc ggcatcatgc gcattttcga tcatatctgg cgctttgata ccgggatgcg
     3001 aagccgcctg ctcagacgct ggcggcgata aattattaca gcagagaagg gacgttctcg
     3061 cgaacagcag ctggccatac accacactga acctggccga tatgcggcag ttgcagcagc
     3121 agcatggtca aacgagactg gccgataccg ccgccgatgg tctgcggcat ttcaccgcgc
     3181 agcagcgcct gatgccattc cagctgcaga cgatcttcat caccggtcag cgccaactga
     3241 tgcttcaacg tgtcggcatc cacgcggatc cccatggaag aaagctcaaa cgcatcttcc
     3301 agtacagggt tccacaccag aatatcgccg ttcagacccg catgacccag ctctgacggg
     3361 gtgctccagt catcataatc cggtgcgcgc acgtcgtggc gatgaccatc gctcagcttg
     3421 ccgccaatcc cgacaaggaa taccgcgcca agatctttcg ctatcgcccg ctcacgccct
     3481 ttggcatcga gatccggata acgagacagt aactcctggc tgtgtacgaa gtggatctga
     3541 tccggcagga acggtgccag gccaaactct tcgctaaccg cagcttcggt tgctttaatt
     3601 cccgcccaga tcgcctctac cgtgcttttc agagtcgaga attgacgctc accgtcgccc
     3661 attacgcgtt cccagtccca ctggtcaaca tagaccgagt gcaacggaga aagacggtct
     3721 tcatcggggc gaagggcttt catgtgcgtg tacagccctt cgcccgcgct gaagtcgtgt
     3781 tgccctaagg tctgacgttt ccacttcgcc agtgaatgaa ccacttcgaa ctgggcatca
     3841 ggcagagctt tcacttttac ctgcaccgct ttttcacagc ccgacaagtt atcctgcgtg
     3901 ccatccccca cacggctaag aatcggcgcc tggacttcga tcagccccag acgttcttcc
     3961 agttggcgag aaaagtgaga tttcacgaag ctaatttggc gttgtttggc aatgtaagcg
     4021 gttttcattt tttatactcc tgcgtcctgt tgcttatgat taagcaacaa aaaccgacaa
     4081 acatgcaata atcattcaat aaaaagccta acacactttt gattcattaa aaaagaaggc
     4141 taaaatagaa tgaatcatca atctgcataa gaaaatccta tggaaaatta tctgatcgac
     4201 aatctggacc gtggcatcct ggaagcatta atgggcaatg cgcgcaccgc ttacgccgaa
     4261 ctggcgaaac aatttggcgt cagtccgggg acgattcacg ttcgagtaga gaaaatgaag
     4321 caggcgggga tcattaccgg ggcgcgtatt gatgtcagcc cgaagcagct cggttatgac
     4381 gtaggctgct ttatcggcat tatattaaag agcgccaaag actacccttc cgcgctggca
     4441 aagctggaaa gccttgctga agtcactgaa gcctattaca caacaggcca ctacagcatc
     4501 tttataaaag taatgtgccg ttcgatcgac gctctccagc atgtacttat caacaagatc
     4561 caaacaattg atgaaattca gtccaccgag acattgatcg tcctgcaaaa cccgatcatg
     4621 cgtaccatca agccataatc ggctttttta atcccatact tttccacagg tagatcccaa
     4681 cgcgttcaca gcgtacaata cgccactctt aataaaggtg gcggtttatg gcagatatca
     4741 ctcttatcag cggcagcacc ctcggcggtg ccgaatatgt agcagaacac ctggctgaaa
     4801 agctggaaga ggcgggtttt accaccgaaa cgctgcacgg tccgctgttg gaagatttat
     4861 ctgcctcggg gatctggctg attatcagct ccacccacgg tgccggagat attccggaca
     4921 acctttctcc tttctatgaa gcattgcagg aacagaagcc cgatctttct gcggtccgct
     4981 ttggcgcaat cggtattggt agtcgtgaat atgacacctt ttgtggggct atcgataaac
     5041 tcgaggcaga actcaaaaat tccggtgcaa aacagacagg cgaaacactg aagatcaaca
     5101 ttcttgatca cgacattccg gaagatccgg cagaagaatg gctgggatcg tggattaatt
     5161 tactcaaata agtatacaga tcgtgcgatc tactgtggat aactctgtca ggaagcttgg
     5221 atcaaccggt agttatccaa ggaacaacgg ttgttcagtt tttgagttgt gtataacccc
     5281 tcattctgat cccagcttat acggtccagg atcaccgatc attcacagtt aatgatcctt
     5341 tccaggttgt tgatcttaaa agccggatcc ttgttatcca cagggtagtg cgatcctaat
     5401 aagagatcac aatagaacag atctctaaat aaatagatct tctttttaat acccaggatc
     5461 ccaggtcttt ctcaagccga caaagttgag tagaatccac ggcccgggct tcaatccatt
     5521 ttcataccgc gttatgcgag gcaatcacca tgttttatcc ggatcctttt gacgtcatca
     5581 tcattggcgg gggtcatgca ggcaccgagg ccgcgatggc cgcggcgcgt atgggtcaac
     5641 agactctgct tttgacacac aatatcgaca ctctggggca gatgagctgc aacccggcga
     5701 tcggcggtat tgggaaggga catctggtaa aagaagtgga tgcactcggc ggtctgatgg
     5761 cgaaagcgat cgatcaagcg ggtatccagt ttaggatact aaacgcaagc aaaggaccgg
     5821 cggttcgcgc tacccgagct caggcggatc gtgtgctcta ccgtcaggcg gtacgtacgg
     5881 cgctggagaa ccaaccgaac ctgatgatct tccagcaggc ggttgaagat cttattgtcg
     5941 aaaacgatcg cgtggtcggt gctgttaccc aaatgggact gaagttccgt gccaaagccg
     6001 tcgtgctcac cgttgggacg ttcctcgacg gtaaaattca tatcggtctg gataattaca
     6061 gcggtggtcg tgctggtgat ccgccgtcca ttccgctttc tcgccgtttg cgtgaactgc
     6121 cgctgcgcgt tggtcgtctg aaaaccggga caccaccgcg tattgatgct cgaactatcg
     6181 actttagtgt gctggcgcaa cagcatggcg ataacccaat gccggtattc tcgtttatgg
     6241 gcaatgcgtc ccagcatccc cagcaggtgc cgtgttatat cactcatacc aacgagaaaa
     6301 cccatgatgt gatccgcagt aacctcgatc gtagcccaat gtacgcaggg gtgatcgaag
     6361 gtgtcggccc acgctactgc ccatcgatcg aagacaaagt catgcgcttc gccgacagaa
     6421 atcagcatca gatcttcctt gaaccggaag ggctgacctc taacgaaatt tatccgaacg
     6481 gtatctccac cagcctgccg ttcgatgtgc agatgcaaat cgtccgctcc atgcagggga
     6541 tggaaaacgc gaagatcgtg cgtccgggtt atgccattga gtatgacttc ttcgatccac
     6601 gcgacctgaa accgacgctg gagagcaagt ttatccaggg gctgttcttt gctggtcaga
     6661 ttaacggcac taccggttac gaagaagccg ctgcgcaagg tttgctggtc ggtcttaacg
     6721 ctgcccgtct gtctgctgac aaagaaggtt gggctccggc gcgttctcag gcgtatctcg
     6781 gcgtactggt tgatgacctg tgcactttag gaaccaaaga accgtatcgt atgtttactt
     6841 cgcgcgcaga atatcgtctg atgctgcgcg aagataatgc ggatctgcgt ttgactgaaa
     6901 tcggtcgtga actgggcctg gtggatgacg aacgttgggc gcgctttaac gagaaacttg
     6961 agaatatcga gcgtgagcgt cagcgtctga aatcgacctg ggtaaccccg tcggcggaag
     7021 ctgcagccga agtgaatgct cacctgactg cgccgctttc ccgtgaagcc agtggtgaag
     7081 atctgctgcg tcgtccggaa atgacttatg aaaaattaac cacgctgacg ccgtttgccc
     7141 ctgcgttgac agacgaacag gcggcggaac aggttgagat tcaggttaaa tacgaaggtt
     7201 atatcgcgcg ccagcaagat gagatcgaaa agcagctgcg taacgagaac accctgctac
     7261 ccgcgacact ggattaccgc caggtatccg gtctttctaa cgaagtgatc gccaaactta
     7321 acgatcacaa accggcctct atcggccagg cttcgcgtat ttctggcgtc acgcctgcgg
     7381 ctatctccat tctgctggtg tggctgaaaa aacagggtat gctgcgtcgt agcgcataac
     7441 gcattaaaaa tgcctggtaa gcacccgctt accaggcaac gcatcaagaa caggtaatca
     7501 ccgtgctcaa caaactctcc ttactgctga aagacgcagg tatttcgctt accgatcacc
     7561 agaaaaacca gcttattgcc tacgtgaata tgctgcataa atggaacaaa gcgtacaacc
     7621 tgacttcggt ccgcgatcct aatgagatgc tggtacgcca tattctcgat agcattgtgg
     7681 tggcaccgta tctgcaaggt gaacggttta tcgatgtcgg caccggacca ggactgccag
     7741 gcattccact ctctatcgtg cgtcctgaag cccatttcac tctgttggat agccttggta
     7801 aacgcgtgcg tttccttcgt caggtgcaac atgagcttaa actggagaat attgagccag
     7861 tacagagcag ggtagaagag tttccttcag agccgccatt tgatggcgta attagccgcg
     7921 cttttgcctc tctgaacgat atggtgagct ggtgccacca tcttcctggt gagcaaggcc
     7981 gtttctacgc gctgaaaggg caaatgccgg aagatgaaat cgctttgttg cccgaagaat
     8041 atcaggtcga atcagtggtt aaacttcagg ttccagctct ggatggcgaa cgtcatctgg
     8101 tggtgattaa agcaaataaa atttaatttt tatcaaaaaa atcataaaaa attgaccggt
     8161 tagactgtta acaacaac
//
LOCUS       DAZPBM010000174         8165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPBM010000174 DAZPBM010000000
VERSION     DAZPBM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.175"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            827..1513
                     /locus_tag="ACHUZQ_003586"
                     /pseudo
     CDS             827..1513
                     /locus_tag="ACHUZQ_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1846..2721
                     /locus_tag="ACHUZQ_003587"
     CDS             1846..2721
                     /locus_tag="ACHUZQ_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR1027195.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2729..3505
                     /locus_tag="ACHUZQ_003588"
     CDS             2729..3505
                     /locus_tag="ACHUZQ_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR1027196.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            3674..5935
                     /locus_tag="ACHUZQ_003589"
     CDS             3674..5935
                     /locus_tag="ACHUZQ_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1027197.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            6004..7179
                     /gene="senB"
                     /locus_tag="ACHUZQ_003590"
     CDS             6004..7179
                     /gene="senB"
                     /locus_tag="ACHUZQ_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR1027198.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(7705..>8165)
                     /locus_tag="ACHUZQ_003591"
     CDS             complement(7705..>8165)
                     /locus_tag="ACHUZQ_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR1027199.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
BASE COUNT         2287 a         1881 c         1894 g         2103 t
ORIGIN      
        1 ttcctctatt tttgtgtaga tagatgttaa tggcactggg tgcaatcaat taagaaaatt
       61 gacgccagac taattattaa taatccagtt atctttttat tcatatcatc tcctttccat
      121 tggttaattt atcggaaatt catccccact aactaaaacg atccacacaa taaaactaaa
      181 tctaattgca ggcaatcaat tccagtcgat atatcatctg tatggaaatc atcgctcatc
      241 actcatcgca gcctgaaagc ccagagcaac ggggcaggtc tgcatataag tggttacacg
      301 gcagaattcc agagggttct gtcactaaat tcagtcactg acaaatttgc atgatgttga
      361 atagacgaga ttttgtgtcg aaatagttgt ttcagtaact gtcactcaat cggtggttta
      421 tgtgactata ttcgcaaaaa ctggtcgcag aaaagtcctg ttttcagctt ggaaacccgg
      481 taatcagaat aatggatagg ggaacaaaaa tctctgtata gaacgctcca ttgagggaga
      541 gaaaacgtgt cattttgacc cgttcggtat tcagaacaca caaaaccgat cgcgggtcgc
      601 tttcgtgcat agttatgcaa cgcgctaaaa acgattctga cgcatttttt atgtttaccc
      661 tgttttttct tgtctttttg agttttttgc gtcagagagc gtctgagagg gcacgtacaa
      721 cgggagttat ggtaaatgga tcgggttttc gggaaggttc gacaggattt gccgttgggt
      781 gtagtgtaag cgactgaaaa acaaacgccc ctgaaatcat gtccagatcc ggcagaacca
      841 gcctcatgcg cagttctggc tggtttttga cgttgatcgc gagggggcag cgattgactg
      901 gagcgaccgg aacgctcccg cgccgaacat caccgtaaaa aatcctgtga acggtcatgc
      961 tcacctgctc tacgcgctca atatcgccgt gagaaccgcg ccggacagct cggttaaggc
     1021 gctgaaatac gccgctgcga ttgaacgttc gctgtgtgag aaactttgtg ctgatgtgaa
     1081 ctacagcgga ctgatttgta aaaatccgtt ccacctggaa tggcaggtga tggagtggcg
     1141 agaggaagcc tataccctcg atgaactggc tgattatctc gatttgagcg cctcagcgcg
     1201 ccgtagcatc gataaacatt acgggatggg gcgaaactgc cacctgttcg aaatgacgcg
     1261 caaatgggct tacagggcga ttcgtcaggg ctggcccgaa ttctcacagt ggcttgatgc
     1321 cgtgattcag cgtgtcgaaa tgtacaacgc atcgcttccc gttccgcttt caccgcctga
     1381 atgtcgggct attggcaaga gcattgcgaa atacacgcac aggaacttca cgccggaaac
     1441 tttcgcacag tatgtggctg atacgcacac attaacgtac gttttcgttc cattggccct
     1501 caccccccga taacgaccca ggtagcgcgc cgtattcccc ccgccggccg ttttcttcga
     1561 catgtacatc aggcgcggaa ctcatcgggg cgcgggaatg aaatcccatt cagactccgg
     1621 atagattcaa gcaagcccac ttatcgtcaa aatcggtgtt gcaaaaacgg gagtgaccat
     1681 agatccgttt tccaagcgga ccccataata tcggctggta aacatcactc gctagtgcat
     1741 tctttttgtt ggtaatgata atcatttaca ttcattcttt gatgttttat cataattaat
     1801 gttttgtcaa taaatatatg gaaacatatt gtttcagatg ggaatatgag aaagttcatc
     1861 cttatttcta tgataacgtt agctagtgtt agtctgggag cctgcagaca gaatgtcacc
     1921 ataaagcagg atgcgccagg gcagaaagct ttcctgactg acgggcagat ttacgatttg
     1981 cacagtggca aaattatttc tagcagcgag cttttggcgg atcttgctac cgctcgacat
     2041 cttattattg gtgaaaaaca cgataatgcc gagcatcatc agattgagct ttggcttatt
     2101 caaaatttgc tcatccagcg accacagggg agtgtgttgc ttgagatgtt gacgtcagag
     2161 caacagcccc gagttaacca agttaaatgt tggctgaaag ataatcctgt ggttcgagat
     2221 agccgtgttc aggaactact taactggcaa aaagggtggt cctgggagat gtatggcgac
     2281 atcgtcatgc agctattacg tgggccttac ccgctgctca atgccaacat tggccgggag
     2341 cagatgttgg cactgtacaa aaaaaacgaa tttcctaaag gaaaaaaatc cactgcacct
     2401 gtggtacaag aagctctgcg tgaaaccatc atatctatgc atgaaggcaa ccttgaaagc
     2461 cagcaactga cgtcaatgct ctctattcag cagcagcggg atcgctacat ggcccgccag
     2521 cttctgagtg ccccggtacc gtcgttgttg attgcaggtg gttatcacgc gtcaaaaagc
     2581 atgggcgtac cgttacacat ggaagatctt gccaccggca cccatccagt ggtgttgatg
     2641 cttgcagaaa aaggaatgaa catcacagta gatcatgctg actacgtgtg gtttgtagcg
     2701 ccagatacaa caaaaaggta aaccatggat gatgaatatt ctccacttct cacagtcggt
     2761 aaaatggagc agctggttta tctgttctct actgcttcat ggactgattt tccttgcgtt
     2821 tatctggcgt ttttccgaag ttcagcccgc tatgtctcct gcaccggcga tcatgctgca
     2881 atgggcagaa attatagagg ctccttcttc tccactttcg cttccggtgg gaatcgctca
     2941 acaggagtcc gcagtgacag aagaaaagca acaaactgag gacagacaac aacgtcctgt
     3001 cactgaagac agtgatgcaa ccattgaaat aactaggaag aaaaagtcat ccgatggaga
     3061 aaagaaaaag acacgtccac ctcgtaagat aaaggctcaa accagcgaca gcaaccccac
     3121 agccgtttcc agtaatgctg cacctcaggc tctggtcgaa tcgtcccgta ttgctgcgcc
     3181 ttttaacagt gactcaacaa agcgagataa ttctgaggca tcttgggaaa gcagagtaaa
     3241 aggccaccta aaccgttata aacgttaccc tggcgatgcc agaaaacgtg cacgaaccgg
     3301 cacagccgtc gtgactttta ccgtaaacac ggaaggcact attgtcagta gtttcttgga
     3361 aatctcttca ggaacccttt ctcttgaccg cgaagctatt gctgttttag aacgcgctca
     3421 accacttccc aaaccaccgc ctgaaatatt ggagggagga ttatttaaag taaaaatgcc
     3481 gattaccttt aagttgaagg agtaaaggga taattggccg ttcaagttca tttaactgaa
     3541 atttaatttt catttttatg ctgaacattt tatcaaactt ttccactgtc gaaaaaatca
     3601 aaaagacagt agaacataat taataaacaa atagttatac ttatctttat taatattata
     3661 cccaaggaaa tttatgaacg ttataaaact ggctatcggc tcaggcatat tattgctcag
     3721 ctgcggtgct tactcacaat ccatcagtga aaaaactaat tccgacaaaa aaggagcggc
     3781 agaattcagt ccgctcagcg tttctgtcgg gaagacgacc agtgagcagg aagctctcga
     3841 gaaaacaggc gcgaccagtt cccggacaac ggacaaaaac ctgcaatcac ttgacgcaac
     3901 agtgcgtagt atgcctggta cttatactca aatagatcct ggtcagggag caatcagtgt
     3961 gaatattcga ggcatgagcg gatttggtcg tgtaaacact atggtcgatg gtattaccca
     4021 gagtttttac ggaacctcta cctccggaac aacgacgcat ggttcaacta acaatatggc
     4081 tggcgtactt atagatccta acttactggt agcagttgat gttacacgcg gtgacagcag
     4141 tggctctgaa gggatcaacg cccttgccgg tagtgcaaat atgcgtacta ttggcgttga
     4201 cgatgtaata tttaacggta atacatatgg ccttcgttca cgtttctctg tcggtagtaa
     4261 tgggctggga cgcagcggaa tgatcgccct tggtggaaaa agcgacgctt ttacggatac
     4321 gggaagcatt ggcgttatgg ctgctgtgag cggcagttct gtgtactcta atttctcaaa
     4381 tggttctgga attaacagca aagagtttgg ttatgataaa tatatgaagc agaaccccaa
     4441 atcccaactg tataaaatgg atatcagacc agacgaattt aacagcttcg aactttccgc
     4501 tcgaacctat gaaaataaat ttacacgtcg tgatataacc agtgacgact attacattaa
     4561 atatcattac accccttttt ctgaattaat tgactttaac gtaacggcca gtaccagtcg
     4621 cggtaatcaa aagtatcgtg atggctcgct gtatactttc tacaaaacct cagcgcaaaa
     4681 tcgttctgac gcgctggata tcaacaatac cagccggttc actgtcgcgg acaatgacct
     4741 ggagtttatg ctgggcagca aactgatgcg tacccgctat gaccggacca ttcactcagc
     4801 ggcgggcgac ccgaaagcga atcaggaatc gatcgagaac aatccgttcg caccctccgg
     4861 ccagcaggat atttcagcgc tgtataccgg gctgaaggtt acgcgcggca tctgggaggc
     4921 agatttcaat ctcaactaca cacgtaacag gatcacaggg tacaagcccg cctgcgattc
     4981 acgcgttatc tgcgtgccac agggtagcta cgatattgac gataaagagg gtggcttcaa
     5041 cccttcagtt cagctttctg ctcaggtaac accatggctt cagccgttca ttggctacag
     5101 caaatccatg cgcgccccga acatccagga gatgttcttc tctaattcag gaggcgcatc
     5161 catgaaccca ttcctgaagc ctgaacgtgc agaaacctgg caggcgggtt ttaacattga
     5221 taccagagat ttactggtcg aacaggatgc cctgcgcttt aaggctctgg cgtaccgcag
     5281 caggatccag aactacatct acagcgagtc ttatctggtt tgttctggag gtcgtaaatg
     5341 cagtatgcct gaggtgattg gcaatgactg ggagggcatt agcgatgaat acagcgacaa
     5401 tatgtacatc tacgttaact cggcaagcga cgttatcgca aagggcttcg aactggagat
     5461 ggattatgat gcaggttttg cttttggccg actctctttc agccagcagc aaacagacca
     5521 gccaacctcc atcgccagca cctactttgg cgcaggggat atgaccgaac tgcccagaaa
     5581 atacatgacg ctggatactg gtgttcgctt cttcgataac gcgttgaccc tgggcactat
     5641 cataaaatac acaggcaagg ctcgtcgcct gtcgcctgat tttgagcagg acgaacatac
     5701 cggcgcaata atcaaacagg atttgccgca gatcccaacg attatcgatc tctatggtac
     5761 ttacgagtac aaccgcaacc taacactgaa actttcggta caaaacctga tgaacagaga
     5821 ttattcggag gcgctgaata agctcaacat gatgccaggt cttggtgacg agacccaccc
     5881 agccaattcc gcgcgtggca gaacatggat atttggcggg gacattcgtt tctgatgaca
     5941 atttccgcta ggttcagaca atacgttttc tctcttatgt caattttatt gcaggaacga
     6001 aaaatgaata ttttcacttt atccaaagca ccgctatacc tgttaatttc actattttta
     6061 cccacgatgg ccatggctat cgatccacct gaacgcgaac tttcgcgatt tgccctgaaa
     6121 acgaattacc ttcagtcccc tgatgaaggc gtctatgaac tggcgtttga taatgccagt
     6181 aaaaaggtgt ttgcagcagt caccgatcgt gtaaatcgtg aagccaataa aggctatctg
     6241 tattcgttta attcagattc gctgaaagtc gaaaataaat acacgatgcc ataccgggca
     6301 ttttcgctgg cgataaatca ggataaacat cagctctata tcggacacac ccagtcagcg
     6361 tccctgcgta tcagtatgtt tgacacccca accggcaaac tggtaagaac cagcgacagg
     6421 ttaagtttta aagcggcaaa cgctgcagat tcgcgttttg agcattttcg ccatatggtt
     6481 tacagccagg attccgatac cctgtttgtg agttatagca atatgctgaa aacggccgag
     6541 ggcatgaagc ctctgcataa gctgttaatg ctcgacggga cgacgcttgc cttaaaaggc
     6601 gaggttaagg atgcttacaa aggtacagcg tatggtctga cgatggatga aaaaacacag
     6661 aaaatctacg ttggcggaag agattacatc aacgaaattg atgcgaaaaa tcagacgctg
     6721 ctgcgtacca tcccgttgaa agatccgaga ccacaaatca caagtgtgca gaatctggcg
     6781 gtggactccg cttctgaccg tgcctttgtg gtggtattcg accatgacga tcgttccggt
     6841 acaaaagatg gactctatat ttttgactta cgcgacggta aacagcttgg ctatgtgcac
     6901 acaggagccg gagctaacgc ggtgaaatac aatccgaaat ataacgaact gtatgtcacc
     6961 aacttcacta gcggcaccat cagcgtagtg gatgccacca aatacagcat cacccgtgaa
     7021 tttaacatgc cggtctaccc aaaccagatg gtgttgtcgg acgatatgga taccctttac
     7081 attggcatca aagaaggctt taaccgcgat tgggatcctg atgtgtttgt ggaaggagct
     7141 aaagaacgta ttctgagcat tgatttgaaa aagtcgtgat cggggggaaa aaacatggga
     7201 taaaaactcc gttattccac taaactggcc ggactaggaa aagcaaacat ctctgtcctg
     7261 ccggtacgcc tgttacgccg ctccatgagg ctgaggaatc tccagaaaac gagacaggca
     7321 cagcattgta cgatcaactg atttgtgcca ataattaaat gaaatcacag ctatgcctgt
     7381 tcatggggtt ctaggccgga accccagatt tttccgattg tttgttttct aactgattat
     7441 tcagtagttt tagagggctg acgctcagtg gaacgaaaac tcacgttaag aggttttgac
     7501 tatgataaaa gtcaaagtag gggtgagttt ggatatggaa attattgtac tataaggaga
     7561 gcatgaaaac ccctggggca gtcactgaat gagggaccag cagcggtaaa ataaccagca
     7621 taatttctgc atggtcgatt tttataccgt gacaggctgt tttttatact tggggtcagt
     7681 ttggatatcg aaaattattg tacgttaagg ttgttttttg accttgtcga tcttaactga
     7741 tctcaaacga cgaaacttac ctgaagtcgg tatccggctg tttttccaga tataatcgcc
     7801 tgtcagattg atatgttccc aacctaatgg tgataaatgc gataatagtt gagcatttat
     7861 tttcatgccc ttacgcttca gagcatcaac cgctctttcc atgtaaactg tgttccataa
     7921 ggaaatagcc gccgtcaata aagtcaatcc actggcgcga taactttgat tttccggttt
     7981 ccgatctcta atctccccca atcgatgcat gaatacagca cgggccagag cattacgggc
     8041 ttctcctttg ttcagcccgg cctggacacg tcgccgcaat gccggatccc gaaaccagtc
     8101 cagcataaac agtgttcgtt ctatacggcc aatctctctc agagctttag ccaacccatt
     8161 ttgtt
//
LOCUS       DAZPBM010000175         8157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPBM010000175 DAZPBM010000000
VERSION     DAZPBM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.176"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHUZQ_003592"
     CDS             <1..254
                     /locus_tag="ACHUZQ_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1027200.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHUZQ_003593"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHUZQ_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1027201.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHUZQ_003594"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHUZQ_003594"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1027202.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHUZQ_003595"
     CDS             complement(2905..3180)
                     /locus_tag="ACHUZQ_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1027203.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHUZQ_003596"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHUZQ_003596"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1027204.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHUZQ_003597"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHUZQ_003597"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1027205.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHUZQ_003598"
     CDS             5140..5466
                     /locus_tag="ACHUZQ_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1027206.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHUZQ_003599"
     CDS             5650..6369
                     /locus_tag="ACHUZQ_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1027207.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHUZQ_003600"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHUZQ_003600"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1027208.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="ACHUZQ_003601"
     CDS             7708..>8157
                     /locus_tag="ACHUZQ_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1027209.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2087 a         1988 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAZPBM010000176         8054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPBM010000176 DAZPBM010000000
VERSION     DAZPBM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.177"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            103..468
                     /locus_tag="ACHUZQ_003602"
                     /pseudo
     CDS             103..468
                     /locus_tag="ACHUZQ_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
     gene            complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="ACHUZQ_003603"
     CDS             complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="ACHUZQ_003603"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1027210.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3236..4069
                     /gene="ppsR"
                     /locus_tag="ACHUZQ_003604"
     CDS             3236..4069
                     /gene="ppsR"
                     /locus_tag="ACHUZQ_003604"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1027211.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4226..5272
                     /gene="aroH"
                     /locus_tag="ACHUZQ_003605"
     CDS             4226..5272
                     /gene="aroH"
                     /locus_tag="ACHUZQ_003605"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1027212.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5404..5595
                     /gene="hemP"
                     /locus_tag="ACHUZQ_003606"
     CDS             5404..5595
                     /gene="hemP"
                     /locus_tag="ACHUZQ_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1027213.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5599..7035)
                     /gene="selO"
                     /locus_tag="ACHUZQ_003607"
     CDS             complement(5599..7035)
                     /gene="selO"
                     /locus_tag="ACHUZQ_003607"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1027214.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="ACHUZQ_003608"
     CDS             complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="ACHUZQ_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1027215.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2073 a         2113 c         1910 g         1958 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccaaaattac cggacgcaaa
      121 aaagatatta ttgtccgcgg cggcgaaaat attagcagcc gtgaagtaga agatatttta
      181 ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa tgcccgatga acgtttaggt
      241 gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc attcattatc gctggaagag
      301 gtggtggctt tttttagccg taaacgggtc gcaaaatata aatatcctga acatatcgtg
      361 gtaatcgaaa aactaccgcg tactgcctca ggtaaaatac aaaagttttt gttaagaaaa
      421 gatattatgc ggcggttaac tcaggatgtc tgtgaagaga ttgaataagt ttcatcttcg
      481 gggatcacat aaccccggcg actgaacgcc gccggggatt tattttattt cttcagttca
      541 gccaagctta accaggtttg caccacggtg tccgggttca gagacaggct atcgatcccc
      601 tcttccatca accatgcggc aaagtcttcg tggtcggacg gaccctgacc gcaaatcccg
      661 acatatttgc cctgtttctt cgcggcacgg atagccatcg acagcagtgc tttcaccgca
      721 tcgttgcgct catcgaacaa ttcagacacc acgccggagt cacggtccag accgagcgcc
      781 agctgcgtca tatcgtttga gccaattgag aagccgtcga aatattcgag gaactgctcg
      841 gccagcaagg cgttggacgg gatttcacac atcatgatga ttttcagccc gttctcacca
      901 cgcttcagcc cctgacgcgc cagttcctca accaccgctt tcgcctgatc tacggtacgc
      961 acgaacggga tcatgatctc aacgttggtc agccccatgt cgttgcgcac acgtttcact
     1021 gcttcgcact ccagcgcgaa gcagtcgcgg aagctgtcgg aaacatagcg tcccgcgcca
     1081 cggaagccga gcatcgggtt ctcttcatct ggctcgtaac gctcaccacc gaccaggttg
     1141 gcatattcgt tcgatttaaa atcagagaga cggacaatga cgcgcttcgg ataaaacgcg
     1201 gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt aaaattcacg cggagaatca
     1261 aaacctttca tcatctcgcg gatttcattt tgcaactgcg gttcctgatc gtcaaactca
     1321 agcagtgcgc gtgggtggac gccaatcata cggttgatga taaattccag acgcgcaagg
     1381 cccacgcctt cgttcggcag gcaggcgaag tcgaaagcac ggtccgggtt accgacgttc
     1441 atcatcactt tcaacggcag atccggcatc atttctacgc tggagctttt cacgctaaat
     1501 tccagcaact ccgcatagac gtaaccggta tcaccttcgg cacaagaaac agtgacgttc
     1561 tcaccgtctt tcatccgttc tgttgcatca ccacagccca ctaccgccgg aatgcccagt
     1621 tcacgagcga tgatcgccgc gtgacaggta cgaccgccac ggttggtgac gatggcagat
     1681 gctttcttca tgatcggttc ccagtccggg tcggtcatgt cagtgaccag cacgtcacca
     1741 ggttcgatgc ggttcatttc gctgatctca tggatgactt tcaccggacc cgcgccgatg
     1801 cgatgaccga tagcacggcc ttcggcgata atcttaccct gtgaatgcag cgtataacgc
     1861 tccatgacct gaccgcgtga gcgcacggtt tccggacgcg cctgcacaat gaacagtttg
     1921 ccagtgtggc catctttcgc ccactcaata tccatcgggc gaccgtagtg tttctcaatt
     1981 tgtacggcct gttttgccag ttcctgcact tcttcgttgg tcagcgagaa gatgtcacgc
     2041 tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt gctcctgggt cggcgcgtaa
     2101 accatgcgga tttttttcga ccccatggtg cgacgcacga tagccgggcg attcgccgcc
     2161 agtgtcggtt tatggacgta aaactcatcc gggttaaccg caccctgcac gaccatttcc
     2221 ccaaggcccc atgcggaagt gataaacacc acctggtcaa agccggattc ggtatcaatg
     2281 gagaacatca cgccagaaga tgcgaggtcg gagcgcacca tccgctgaac accggcagag
     2341 agcgccacgc cacggtgatc gtaaccctgg tgcacacgat aagagatggc gcgatcgtta
     2401 aacagagaag caaatacatg cttcactgcc acgagaacgg cgtcaaaacc ctgaacgttg
     2461 aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca tatcttctgc ggtggcggag
     2521 gagcgcaccg caaaagaggc gttttcgtca tcggcggaaa gctgtgcata ggcttcgcgg
     2581 atggcgtttt ccagctcagg ctggaaggga gtgtcgataa tccactggcg gatttgcgcg
     2641 cccgctttcg caagctgagt aacatcgtca atatccgttt tatccagcag ttcataaatg
     2701 cgctggttta cgccgctttg gtccagaaac tggttaaacg cgtcggcggt tgtggcgaaa
     2761 ccattcggaa cggaaacacc cattccggaa aggttagtaa tcatttcacc cagggaggca
     2821 tttttgcccc caaccctgtc tacatcattc atgccgagtt ggttatacca aagcaccagc
     2881 ggtgacgagc cattgttgga catcgaacaa tccttttgtg ataaatgaac ggtttgagaa
     2941 acacatttct gcgcatttat ctttgcatat ttaaccggat gaaaaaaacg gtgaatcgtt
     3001 caagcaaata tattttttta ctttttaaga ctgatcccag cgttgcgcaa atctgctctc
     3061 ttcgacaatt tccacataaa ccatcggtat aaacgaaacc ataaaaatga aatgctgttt
     3121 tcataaaaaa taaaattgaa ggttcatttt ataaaccaga cataacgtcc tacgcttctg
     3181 tgcgttttta atttatgctt tcatagaatt atgtctgcat cacgggaaga acaaaatgga
     3241 taatgctgtt gatcgccacg ttttttatat ttctgatggt acggcaataa ctgcggaggt
     3301 attaggacac gcagtaatgt cacaatttcc cgtcactatc agcagcatta cgctgccgtt
     3361 tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag attgacgcaa tttatcacca
     3421 gacaggtgtg cgcccgctgg tcttctactc catcgtgttg ccggagattc gcgccatcat
     3481 cttgcaaagt gaaggctttt gccaggatat cgttcaggcg ctggttgccc cgctacaaca
     3541 agagatgaaa ctggatccaa cgccgattgc tcatcgtacc catggcctta accctaataa
     3601 tctcaataaa tatgatgcgc gcattgcggc gattgattac accctcgccc acgatgacgg
     3661 catttcgttg cgcaatctgg atcaggctca ggtgatcctg ctcggtgttt ctcgctgtgg
     3721 taaaaccccc accagtcttt atctggcaat gcaatttggt atccgcgcgg caaactaccc
     3781 ctttattgcc gacgatatgg ataatctggt gctacccgcg tcgctcaaac cgcttcagca
     3841 taaattgttc ggcctgacta tcgacccgga acgtctggcg gcgattcgcg aggaacgtcg
     3901 ggagaacagt cgctatgcct cgcttcgtca gtgcaggatg gaagtcgcgg aagtggaagc
     3961 gttgtaccgt aaaaatcaga tcccgtggat taacagtacc aattattcgg tagaagagat
     4021 tgccaccaag atcctcgata tcatgggcct tagtcgccga atgtactaga gaactagtgc
     4081 attagcttat ttttttgtta tgatgctaac cacccggcga ggtgtgacac acctcgcact
     4141 tgaaatcagc agcgattggt ttatcgtgat gcgcatcact tcccggcagt cctgccgtag
     4201 aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat
     4261 tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgccgc
     4321 cacccatgtc accgactccc gccgcagaat tgaaaaaata ctcaatggtg aagataagcg
     4381 actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc
     4441 cccccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac
     4501 ctattttgaa aaaccacgaa ctgttgtcgg ctggaaagga ctaatctccg atccagattt
     4561 aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt
     4621 aaatgagctg ggcgtcccaa ccgcgactga gttcctcgat atggtgaccg gtcagtttat
     4681 tgctgattta atcagttggg gcgcgattgg cgcacgtact accgaaagtc agatccaccg
     4741 tgaaatggct tcggcactct cctgtccggt aggttttaaa aatggtaccg atggcaatac
     4801 gcggattgct gtggatgcta tccgcgcagc ccgcgccagc catatgttcc tctcgccaga
     4861 caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat
     4921 gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct
     4981 gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa
     5041 gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc
     5101 tacggcgatt gctggaatta tggcggaaag tttcctgctc gaaggaacgc aaaaaatcgt
     5161 cggcagtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga
     5221 taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc
     5281 ccattcctga cggaatgggc atttctgcgc aacttgttgt cttctcaaca aattactgct
     5341 tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt attattaatc
     5401 tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggacgccaa tcacaaggcc
     5461 gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa
     5521 ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg
     5581 ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg
     5641 tggacgactg acatagtcat catctctatc gctgaaagga tttcgcaacg ccccatgcag
     5701 gcggtgcaat tccgtcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca
     5761 gttgcgcaat accagagcgg ggttaacgct ttgcatcaac tgctgacgct cactatcgct
     5821 aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagccatcaa atgccgcacg
     5881 atcaataaac tcatcacgta gcggtgacgc cgtgctgtgc tgctcggtca gactcagcat
     5941 gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag
     6001 tagcgcgtta tcctcttttt gctccgtgat gaagcccagt ttctgccgca tccgttgtcc
     6061 ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc
     6121 aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg
     6181 attatcaaaa ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta
     6241 atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc
     6301 ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt
     6361 acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga
     6421 ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta
     6481 gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc
     6541 gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg
     6601 ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga
     6661 acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag
     6721 atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc
     6781 atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg
     6841 tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga
     6901 cgatggaata ctcagcgtgt tagccagttc ggtattatgc caaatcagcc gggcattatt
     6961 taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac
     7021 aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac
     7081 accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca
     7141 ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct
     7201 gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctggatc
     7261 gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc
     7321 agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc
     7381 aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt
     7441 ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat
     7501 agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta
     7561 agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac
     7621 tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc
     7681 gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg
     7741 acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa
     7801 aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga
     7861 tgaggccata agacataaca attacagaag gagtaacttt catttgttcc atgttaacca
     7921 ctttttcagg ggtccttttt ttagattatc ctgattataa acgaataatc catttacgga
     7981 atttttgtct gcaaaatact actgtattca ggagtaaatg gacggcgatg tatcatcgcc
     8041 gtccattttt cgac
//
LOCUS       DAZPBM010000177         7975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPBM010000177 DAZPBM010000000
VERSION     DAZPBM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.178"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            127..216
                     /gene="kdpF"
                     /locus_tag="ACHUZQ_003609"
     CDS             127..216
                     /gene="kdpF"
                     /locus_tag="ACHUZQ_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1027216.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            216..1889
                     /gene="kdpA"
                     /locus_tag="ACHUZQ_003610"
     CDS             216..1889
                     /gene="kdpA"
                     /locus_tag="ACHUZQ_003610"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1027217.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1912..3960
                     /gene="kdpB"
                     /locus_tag="ACHUZQ_003611"
     CDS             1912..3960
                     /gene="kdpB"
                     /locus_tag="ACHUZQ_003611"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR1027218.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3969..4541
                     /gene="kdpC"
                     /locus_tag="ACHUZQ_003612"
     CDS             3969..4541
                     /gene="kdpC"
                     /locus_tag="ACHUZQ_003612"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1027219.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4534..7218
                     /gene="kdpD"
                     /locus_tag="ACHUZQ_003613"
     CDS             4534..7218
                     /gene="kdpD"
                     /locus_tag="ACHUZQ_003613"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR1027220.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7215..7892
                     /gene="kdpE"
                     /locus_tag="ACHUZQ_003614"
     CDS             7215..7892
                     /gene="kdpE"
                     /locus_tag="ACHUZQ_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR1027221.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1628 a         2089 c         2360 g         1898 t
ORIGIN      
        1 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
       61 ttgcgaaatc tttgcagcca gaattctact cttccggtat cacttttagg ccactggagg
      121 tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg tgtttttatt actgggttat
      181 ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      241 ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
      301 ggctgattaa tgacattcct cttccaggta cagcgggcgt tgaacgtata ctctttcgtt
      361 tacctggcgt ctctgaccat gagatgaact ggaagcaata tctttgtgcc attctcggcc
      421 tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
      481 ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatacc gccgtcagct
      541 ttgtcacgaa taccaactgg caatcttata gcggtgaaac cacgttgagc tatttcagcc
      601 agatggcagg cttaacggta caaaactttc tttctgccgc cagcgggatt gcggtgattt
      661 ttgccttcat ccgtgcgttt actcgccaga gcatgagcac actcgggaat gcctgggtcg
      721 atctgctacg catcacgtta tgggtgctag tccctgtggc gttgttgatc gcactgtttt
      781 ttattcaaca aggtgcgctg caaaactttc tgccctatca ggcagtgaat accgttgaag
      841 gagcgcaaca gctgttaccc atgggaccag tagcttctca ggaagcgatc aagatgctcg
      901 gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
      961 cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacagcg ctgtgctttg
     1021 cctttggtga agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag
     1081 tgatttttgt catctgcgta ggcgtggtta tgtgggcaga agttcagggt aatcctcatc
     1141 tgctggcgct cggcgcagac agcagcatca atatggaagg taaagagagc cgtttcggcg
     1201 tgctggtcag tagcctgttt gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg
     1261 cgatgcatga ttcgtttacc gctctcggcg gcatggtgcc gatgtggcta atgcaaattg
     1321 gtgaagtggt gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc
     1381 tggcggtgtt tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa
     1441 tcgacgtacg cgagatgaaa ctgattgcac tggcgattct ggttactcca acactggtac
     1501 tgatgggcgc ggcgttggcg atgatgaccg acgccggacg tagcgccatg cttaaccctg
     1561 gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcgtc cgccgctaac aacaacggca
     1621 gcgcctttgc cggattaagc gccaactctc cgttctggaa ctgtttactg gcgttctgca
     1681 tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga
     1741 gtaaaaagag ccaacccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg
     1801 gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc
     1861 ttggtccggt ggcggaatat ctctcctgat gatattgagc gagcactgaa tatgagtcgt
     1921 aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa
     1981 aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt
     2041 ctgctgacca cctgtattag catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg
     2101 tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag
     2161 gcgctggcag aaggccgcag taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact
     2221 gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc
     2281 gatcaactgc gtaaaggtga catcgtgctg gtagaagctg gcgatattat cccctgcgat
     2341 ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca
     2401 ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt
     2461 tctgactggc tggtgattga gtgtagcgtt aaccccggtg agacatttct ggatcggatg
     2521 atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga acgagattgc cctgactatc
     2581 ctgctgattg ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc
     2641 gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc
     2701 ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg
     2761 ggcgcgaatg tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt
     2821 ctgctactgg ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc
     2881 cccgcgcagg gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct
     2941 gatgaaacgc cggaaggtcg cagtattgtg atcctcgcca agcaacgttt taacctgcgc
     3001 gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg
     3061 agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc
     3121 catattgagg ctaacggcgg tcacttccct gccgatgttg atcaaaaagt cgatcaggtt
     3181 gcgcgtcagg gagccacgcc gctggtggtg gtggaaggtt ctcgtgtgct gggcgttatt
     3241 gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg
     3301 ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc gatagctgcg
     3361 gaagcgggtg tcgatgattt tctcgccgaa gcaacaccgg aggccaagct ggcattgatc
     3421 cgtcagtatc aggcggaagg tcgtttggta gcgatgaccg gcgacggcac caacgatgct
     3481 ccggcgctgg cgcaggcaga tgtcgcggtg gcgatgaact ccggcaccca ggcggcgaaa
     3541 gaggcgggca atatggtgga tctcgactct aacccgacca agttgatcga ggtggtgcac
     3601 attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat
     3661 gtggcgaaat acttcgccat tattccggcg gcgttcgcgg caacatatcc gcagttaaat
     3721 gcgctgaaca tcatgcgcct gcattcgccc gactccgcca ttctcagtgc ggtcattttc
     3781 aacgccttga ttatcgtctt tttgattcct ctggcgttaa aaggcgtgag ttataaaccg
     3841 cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg
     3901 gtgccgttta ttggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga
     3961 ggtttaccat gagtggatta cgtccggcat tatcgacatt tctctttctg ttattgatta
     4021 ctggcggcgt ttacccgctg ctgaccaccg cactggggca atggtggttt ccctggcagg
     4081 ccaacggttc gttgatccgt gaaggtgata cggtgcgtgg ctcggcatta atcgggcaga
     4141 attttaccgg caacggctat tttcatggtc gcccgtcagc aacggctgaa atgccctata
     4201 atccacaggc ttctggcggg agcaatctgg cggtcagtaa ccctgagctg gataaactaa
     4261 tagccgcacg cgttgctgca ttacgggccg ctaacccgga tgccagcgcg agcattccgg
     4321 ttgaactggt gacggcatcg gcaagcgggc tggacaataa tatcaccccg caagcggcgg
     4381 cctggcagat cccacgcatc gcgaaagcgc gtaatctcag cgttgaacag ctcacgcagc
     4441 tgatcgcaaa atacagccaa caaccgctgg tgaaatatat cggccagccg gtcgtcaaca
     4501 ttgtcgaact caatctggcg ctggataagc ttgatgaata acgaaccctt acgtcccgac
     4561 cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa agttttcttc
     4621 ggtgcctgtg caggcgtcgg gaagacctgg gcgatgctgg cagaagccca gcgactgcgg
     4681 gcgcaagggc tggatattgt ggttggcgtg gtagaaaccc acgggcgaaa agataccgcc
     4741 gcaatgctgg aagggctggc tgttctgccg ccaaaacgcc tggcgtaccg tgggcggcac
     4801 atctgcgagt ttgacctcga tgccgccctc gcccgccgtc cggcgctgat cttaatggac
     4861 gaactggcgc acagtaatgc gccaggttct cgtcacccta aacgctggca ggacatcgaa
     4921 gagctgctgg aagcaggcat tgatgttttc actaccgtca acgttcagca tctggaaagt
     4981 ctgaatgatg tggtcagcgg cgtcaccgga attcaggtac gggaaaccgt gcccgatcct
     5041 tttttcgatg ccgccgacga cgtggttctg gtggacttgc ccccggacga tctgcgccag
     5101 cggctgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat tgaacatttt
     5161 ttccgcaaag gcaatctgat cgccctgcgc gaactggcac tgcgccgtac tgccgatcgc
     5221 gttgatgagc aaatgcgcgc ctggcggggg catcctagcg aagagaaagt ctggcacacg
     5281 cgcgacgcga tccttttatg catcggccat aacaccggta gtgagaagct ggtccgcgcg
     5341 gcggcgcggc tggcgtcacg gctgggtagc gtctggcacg cggtgtatgt tgaaacccct
     5401 gccctgcatc gcttaccgga gaaaaaacgt cgtgcaattc tcagcgcctt acgtctggcg
     5461 caggaactgg gcgcggagac ggcaacactt tctgatccag cggaagagaa agcggtagtg
     5521 cgttatgccc gtgaacataa tctcggcaag attattctcg gtcgcccggc ctcgcgccgc
     5581 tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga tctcgatcag
     5641 gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc ggatagccgc
     5701 tcttttaaag acaagtggcg ggtacagatt cagggatgcg tggttgccgc cgcgttatgc
     5761 gccgttatca ccttaattgc catgcagtgg ctgatggcgt ttgatgccgc caacctggtg
     5821 atgctgtatc tgcttggcgt ggtggtggtg gcgctatttt atggacgctg gccttcggtg
     5881 gttgccaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc acgcggcacg
     5941 ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg
     6001 gtgatcggga accttactgc tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgag
     6061 caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag
     6121 gacatcgccg ccaccagcga acaatttatt gcctccacgt ttcatgcccg cagtcaggtg
     6181 ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg aatgacgcca
     6241 tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg cgcgggcacc
     6301 gacacgttac ccggtgtacc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac
     6361 gggctggtgg tggtggagcc ggggaatctg cgccagttga tgatcccgga acagcagcgc
     6421 ctgctggaga cgtttacgct gttagtcgcc aatgcccttg agcggctgac gctaaccgcc
     6481 agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggca
     6541 gcgctttcgc atgatttacg cacgccgctt acggtgctgt ttggtcaggc agaaatctta
     6601 acgctcgatc tggcaagcga aggatcaccc cacgcccgcc aggccagcga gattcgtcag
     6661 catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg aattcagtcc
     6721 ggcggcttta atttgaagaa agagtggtta acgctggaag aagtagtcgg cagcgcgctg
     6781 caaatgctgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga accgctgacc
     6841 ttaatccacg ttgacgggcc actctttgaa cgggtgctga ttaatctgct ggagaacgcg
     6901 gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg cccactttga gggcgaaaat
     6961 ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga gcagacgata
     7021 tttgataagt ttgctcgcgg gaataaagag tcggcggtgc cgggtgtcgg gcttgggttg
     7081 gcaatttgtc gggcgattgt ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg
     7141 gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga acttgaagac
     7201 tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct
     7261 ttctgcgcac ggcgctggag ggcgacggga tgcgcgtctt tgaggccgaa actctgcaac
     7321 gcggcttgct ggaagcggca acccgtaagc cagatttgat tattctcgat ctcggcctgc
     7381 ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcccg gtgccggtga
     7441 ttgtgctttc cgcacgcagc gaagagagcg acaagatcgc cgcgctggat gccggagcgg
     7501 atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt
     7561 tacgccgcca ctccgccacc gccgcgcccg atccgctggt gaaattttcc gatgttaccg
     7621 tcgatttagc cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg
     7681 agttccgcct gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc
     7741 tccttaacca ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata
     7801 tgggacatct gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg
     7861 aaaccggtat tggatatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata
     7921 gtgattaatt tattattaaa cctgcgccga atattattat ttggcaggtt taatt
//
LOCUS       DAZPBM010000178         7935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPBM010000178 DAZPBM010000000
VERSION     DAZPBM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.179"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            124..735
                     /gene="ruvA"
                     /locus_tag="ACHUZQ_003615"
     CDS             124..735
                     /gene="ruvA"
                     /locus_tag="ACHUZQ_003615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1027222.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            744..1754
                     /gene="ruvB"
                     /locus_tag="ACHUZQ_003616"
     CDS             744..1754
                     /gene="ruvB"
                     /locus_tag="ACHUZQ_003616"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1027223.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1901..2686)
                     /gene="znuB"
                     /locus_tag="ACHUZQ_003617"
     CDS             complement(1901..2686)
                     /gene="znuB"
                     /locus_tag="ACHUZQ_003617"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1027224.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2683..3438)
                     /gene="znuC"
                     /locus_tag="ACHUZQ_003618"
     CDS             complement(2683..3438)
                     /gene="znuC"
                     /locus_tag="ACHUZQ_003618"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1027225.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3517..4449
                     /gene="znuA"
                     /locus_tag="ACHUZQ_003619"
     CDS             3517..4449
                     /gene="znuA"
                     /locus_tag="ACHUZQ_003619"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1027226.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4465..5787
                     /gene="mepM"
                     /locus_tag="ACHUZQ_003620"
     CDS             4465..5787
                     /gene="mepM"
                     /locus_tag="ACHUZQ_003620"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1027227.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5907..6878
                     /gene="lpxM"
                     /locus_tag="ACHUZQ_003621"
                     /gene_synonym="msbB"
     CDS             5907..6878
                     /gene="lpxM"
                     /locus_tag="ACHUZQ_003621"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1027228.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6966..7935)
                     /locus_tag="ACHUZQ_003622"
                     /pseudo
     CDS             complement(6966..7935)
                     /locus_tag="ACHUZQ_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2109 a         1908 c         2010 g         1908 t
ORIGIN      
        1 atactgtgcc atttttcagt tcatcgagac acctcgcaag ttttcttcat ccttcgctgg
       61 atatctatcc agcatttttt tatcatacag cattatcttt gattcattac gcaggagcgt
      121 catgtgatag gcagactcag aggcatcatc attgaaaaac aacccccgct ggtgttaatt
      181 gaagtgggcg gcgtaggcta tgaagtgcat atgccgatga cctgttttta tgaactccct
      241 gaagcgggtc aggaagcgat cgttttcacc cactttgtgg tgcgtgaaga cgcgcaactg
      301 ctgtacggtt ttaacaataa acaagagcgc acgttgttta aagagttaat caaaaccaac
      361 ggcgtcggcc cgaagttggc gctggcgatc ctctccggaa tgtcagcgca gcagttcgtt
      421 aatgccgttg aacgtgaaga agtgggagca ctggtgaaac tgccgggcat tggtaaaaaa
      481 accgccgagc gtttaattgt cgaaatgaaa gaccgattta aaggtttgca cggcgatctc
      541 tttacgccag ccgccgatct ggtgctcacg tcgcctgcca gcccggcaac cgacgatgct
      601 gaacaggaag cggttgccgc gctggtggcg ctgggctata aaccacaaga agcaagccgc
      661 atggtgagca aaatcgctcg cactgacgcc agcagtgaaa cattaattcg cgaagcccta
      721 cgcgccgcgt tatgaggtaa aggatgattg aagcagaccg tctgatttct gccggtacca
      781 ctttgccgga agatggggca gatcgcgcca ttcgccctaa attactggaa gagtatgttg
      841 gtcagccgca ggttcgttca cagatggaga ttttcatcaa agcagcgaaa ctgcgcggcg
      901 atgccctcga tcatctgttg atttttggtc ctccggggtt gggtaaaact acgcttgcca
      961 acattgtcgc caatgaaatg ggcgttaatt tacgcacgac ttctggtccg gtgctggaaa
     1021 aggcgggcga tctggcagcg atgctcacta accttgaacc gcatgacgtg ctgtttattg
     1081 atgagatcca ccgtctatcg ccagttgttg aagaagtgct gtacccggca atggaagact
     1141 accaactgga tatcatgatt ggtgaaggtc cggcggcacg ctccattaaa attgatttgc
     1201 cgccgtttac cctgattggt gcaaccacgc gcgcaggttc gctgacatca ccgttgcgcg
     1261 accgttttgg tattgtgcaa cgtctggagt tttatcaggt gccggatctg caatatatcg
     1321 tcagtcgcag cgcacgcttt atggggcttg agatgagtga tgacggcgcg ctggaagttg
     1381 ctcgtcgcgc tcgcggtacg ccacgtattg ccaaccgtct gctgcgtcga gtgcgtgatt
     1441 tcgccgaagt gaagcacgat ggcaccatct cggcagatat cgctgctcag gcgctggata
     1501 tgttgaatgt cgatgctgaa ggtttcgatt atatggaccg caaattgttg ctggcggtaa
     1561 tcgataagtt ctttggtggg ccggtaggtc tggataacct ggcggcagcc attggcgaag
     1621 aacgtgaaac cattgaggat gtgctggagc cttatctgat tcagcaaggc tttttgcagc
     1681 gtacaccgcg tgggcgtatg gcgacgacgc gggcgtggaa tcactttggc ataacgccgc
     1741 cagaaatgcc gtaagtcgga ttggtcgctc gcaccgcatc cgacattggc agtgtgcctg
     1801 atgcgacgct attcgcgtct tatccggcct accagagata gcgaaccgta ggtcggataa
     1861 ggcgctcgcg ccgcatccga caaatgtgtt cagcgacaga ttagctggcc tgctttttca
     1921 tcatactgat aataaataac agtgcagcac atagcactac cgacggaccc gccggcgtat
     1981 cgtaaaacgc ggaaaaggtt aaaccgccag ttactgccac catccccacc aaaacagcga
     2041 caccagccat ctgttccggc gtacgggcaa agcgacgcgc agtagctgca ggaataatca
     2101 gcaacgaagt aataatcaac gcaccgacaa atttcatcgc tacaccaatc gtcaatgccg
     2161 tcaccagcat caacaacaat ttcacgcgct gtaatttcac accatcaaca aacgccagat
     2221 ccgggctaat cgtcatcgac agcaaattgc gccattgcca gaacaaaata gccaccacga
     2281 tgaccacgcc aatcgcaata gagatgagat cttctggcgt cactgccagc aaatcaccga
     2341 acaggtaagc catcaaatca acacgaatat tagacatcag actaacgacc accaggccca
     2401 gcgacagggc actgtgcgcc ataatcccta ataacgtgtc gatcgccagc tgtggacgct
     2461 tctccagcca taccagaccg cccgccagca gcagcgtaac ggcaatcacc gcatagaatg
     2521 gattcacgtc cagcaacaaa ccaaacgcga caccaagtaa tgaggcatga gccagcgtat
     2581 caccgaaata agacatacga cgccagacta caaacgaacc cagcggaccc gcggcacagg
     2641 cgagcatgat cccggctaac caaccgggaa ataataattc aatcatgagc gatcatttcc
     2701 ccgacgcaaa acaattcgtc cctgtaaatc gtgacgatgg ttatgatgat ggcgatagat
     2761 acccagttgt tcagcaccac gaggaccaaa cattgaaata aactccggat gcagggaaac
     2821 aacttccggt gtgccggaac aacaaatgtg gtgattcagg cacagcactt catcggtttt
     2881 tgccatcacc agatgcagat cgtgagaaac cattaaaacg ccacaatcca gttcacgacg
     2941 cagttggtca ataaggtcat ataacgccac ctggccattt acatccacgc cttgagtggg
     3001 ttcatccagc actaataatt gcgggcgatt taacaatgct cgcgctaaca gtacacgctg
     3061 cgtttcgcca ccagagagct tttgcatcgg tgcgttaatc aggtgcccgg cctggacacg
     3121 tttcagtgca ggcaaaatat cttctttatg tgtaccaggg cgtaagcgta aaaaacggtt
     3181 tacggtcagt ggcaacgtgg tgtcgagata cagcttctgc ggtacatagc cgatgcgcag
     3241 ttttccgttg cgcttgataa ccccttcatc gggtgttacc agcccgagca ctacccgtac
     3301 cagtgtcgac ttacctgcgc catttggccc aagtaaagtc aaaatttttc caggtttaag
     3361 ttccagcgac acatcagaga ggacgcggcg ttggccaaaa gaaaccgaga cattttccaa
     3421 ggaaaccaga cttgtcatgt taattttagt cttgcagtag tcatgaaatg ttataatatc
     3481 acacttctca tattcattac gatgattggt cgcattatgt tacataaaaa aacgcttctt
     3541 ttcgcagcat tatccgccgc tctctgggga ggtgcaacac aggccgcaga tgccgccgtt
     3601 gtcgcttcgc ttaaacccgt tgggttcatc gcttctgcca ttgctgatgg ggtaacagaa
     3661 acagaggttt tacttcctga cggcgcttca gaacatgatt attcactgcg cccatcggat
     3721 gtaaaacgct tacagaacgc ggacttagtc gtttgggttg gcccggagat ggaagcgttt
     3781 atgcaaaaac cggtaagcaa attaccagga gcgaagcagg taacgattgc gcagcttgaa
     3841 gatgtgaaac cgctactgat gaaaagtatt cacggcgatg atgatgatca cgaccacgcg
     3901 gaaaaaagtg acgaagatca ccatcacggc gatttcaaca tgcatctttg gctttcccca
     3961 gagatagcgc gggctacagc ggttgcaatc catggaaaat tagtggaact tatgccgcaa
     4021 agtcgagcca aacttgacgc caacctgaag gattttgagg cacaattagc ctcaaccgaa
     4081 acgcaggttg gtaacgagct cgcgccgctc aaggggaaag gttatttcgt ttttcacgat
     4141 gcttacggct atttcgaaaa acagttcgga ctgacaccgc ttggtcattt taccgttaac
     4201 cctgagattc aacctggcgc gcagcgttta catgaaataa gaacacagtt ggttgagcaa
     4261 aaagcaacct gcgtttttgc tgagccacag ttcaggccag cggtcgttga gagcgtcgca
     4321 cgagggacat ccgttcgtat gggaacgttg gatcccctgg ggacgaatat caaactgggt
     4381 aaaacaagct attcagaatt cctgagtcaa ttagccaacc agtatgcgag ctgcctgaaa
     4441 ggagattaat gaggaagtga ttacgtgcaa cagatagccc gctctgtcgc cctggcgttt
     4501 aataatttac cgcgaccaca ccgcgttatg ttggggtcgc tcaccgttct tactctggcc
     4561 gtcgctgtct ggcggcccta tgtttatcac cgcgatgcca cgccaattgt caaaaccatt
     4621 gagctggaac agaacgaaat tcgttcgctc ttacctgaag ccagtgagcc gattgatcaa
     4681 gctgcacaag aagatgaagc cattccccag gacgaactgg atgacaaaat cgccggtgaa
     4741 gcgggcgtgc atgaatatgt tgtttccact ggcgatacgc taagcagcat tctcaatcag
     4801 tatggtattg atatgggtga tatcacccaa ctggctgcgg ccgacaaaga attgcgtaac
     4861 ctgaaaatcg gtcaacaact ctcctggaca ttaaccgcgg acggcgaact gcagcgcctc
     4921 acctgggaag tgtctcgtcg tgaaacccga acctatgacc gtactgccgc taacggtttt
     4981 aaaatgacca gcgaaatgca gcaaggagag tgggttaaca atctgctgaa aggtaccgtc
     5041 ggggggagct ttgttgccag cgccagaaac gccggtttaa ccagcgccga agtgagcgca
     5101 gtgattaaag ccatgcagtg gcaaatggat ttccgcaaac tgaaaaaagg cgatgaattt
     5161 gcggtgttaa tgtctcgaga aatgcttgat ggtaaacgtg agcaaagcca gctgctgggc
     5221 gtacgtttgc gttcagaagg taaagattat tacgctatcc gcgctgaaga tggcaaattc
     5281 tacgatcgta acggtactgg tctggcgaaa ggattcttgc gattcccgac ggcgaaacag
     5341 ttccgtatct cgtctaactt taacccgcgt cgtactaatc cggtgaccgg tcgcgttgca
     5401 gcacacagag gtgttgattt cgccatgccg cagggtacgc cagtgctttc agtgggtgac
     5461 ggtgaagtgg tggttgccaa acgcagtggc gcagcaggtt attatgtggc tattcgtcat
     5521 ggtcgcagct acaccacgcg ttatatgcac ttgcgcaaga ttctggtgaa accgggacag
     5581 aaggtgaaac gtggcgaccg tatcgcgctt tccggtaata ccggacgttc aaccgggccg
     5641 catctgcact atgaagtatg gataaaccag caggccgtaa acccgctgac ggcaaaactg
     5701 ccgcgtaccg aagggctgac cggctccgat cgtcgcgaat tcctggcgca ggccaaagag
     5761 attgtgccgc agctacggtt tgattaatta acatccattc gcagccggta cgcagtcagt
     5821 atcggctttt tttatttggt gcggggcaag ttgcgccgct acactatcac cagattgatt
     5881 tttgccttat ccgaaactgg aaaagcatgg aaacgaaaaa aaataatagc gaatacattc
     5941 ctgagtttga taaatccttt cgccacccgc gctactgggg agcatggctg ggtgtagcag
     6001 cgatggcggg tatcgcttta acgccgccaa agttccgtga tcccattctg gcacggctgg
     6061 gacgttttgc cggacgactg ggaaaaagct cacgccgtcg tgcgttaatc aatctgtcgc
     6121 tctgctttcc agaacgtagt gaagctgaac gcgaagcgat tgttgatgag atgtttgcca
     6181 ccgcgccgca agcgatggca atgatggctg agttggcaat acgcggaccg gagaaaattc
     6241 agccgcgcgt tgactggcaa gggctggaga tcatcgaaga gatgaggcgt aataacgaga
     6301 aagttatctt tctggtgccg cacggttggg ccgtcgatat tcctgccatg ctgatggcct
     6361 cgcaagggca gaaaatggca gcgatgttcc ataatcaggg caacccggtt tttgattatg
     6421 tctggaacac ggtgcgtcgt cgctttggcg gtcgtctgca tgcgagaaat gacggtatta
     6481 aaccattcat ccagtcggta cgtcaggggt actggggata ttatttaccc gatcaggatc
     6541 atggcccaga gcacagcgaa tttgtggatt tctttgccac ctataaagcg acgttgcccg
     6601 cgattggtcg tttgatgaaa gtgtgccgtg cgcgcgttgt tccgctgttt ccgatttatg
     6661 atggcaagac gcaccgtctg acgattcagg tgcgcccacc gatggatgat ctgttagagg
     6721 cggatgatca tacgattgcg cggcggatga atgaagaagt cgagattttt gttggtccgc
     6781 gaccagaaca atacacctgg atattaaaat tgctgaaaac tcgcaaaccg ggcgaaatcc
     6841 aaccgtataa gcgtaaagat ctttatccta tcaaataaaa aaagcctctc aaattattga
     6901 gaggctgtac tcgataaaaa atctcaatga gtgttcccac tattttgagt ttttaggtta
     6961 gtacgctatt gagttaatcg tagcgtgata atttcaaaag gtttgaactc aagattgcaa
     7021 tattgattgc aataaaaact gtcatctaac ttattttctt gaagatcgac atattgaacg
     7081 tagtgataat tacatactca cgaaagcatg gctgaaattt ctaatccatg actctcatat
     7141 aatcttacga ttataccgtc gccgtcttca gctaatttta ttgtttcgat gataacgctg
     7201 ttagtatcga tagcaaaaaa tgatagtgaa gggggtaatt cacccgggtg cggtttgaca
     7261 gggacgacct gtaagggatg attcattata taggcttgat taataacacc acccacacgg
     7321 taatctccat gatgaggaaa taaggagaat atgaattttt gtttaccaat atctgcaaaa
     7381 ttattaatca ggaaattatc ataattgtct gtcttaacaa cttccccagg tttatgctgc
     7441 gaacgcagca aagttaattc aagcgtctgg tctttgacac gatagccata tttgtttttt
     7501 gctattaaag ctatgccagt ttctcgatca gaaaggtcga cccagtgatg tgctggtatt
     7561 tcatctttgg caaaagcgaa cgaatcatcg ctatgcgttg gtctgtcaat tacaccgaac
     7621 tgaatttgac attgtgcaag agatgtttgt actgacaccg ggaattttac tttgagagaa
     7681 acattaggtt cgtgccaatc aataaacgtt tggacatcaa cgcgtggact atcctgtgtc
     7741 agggtaaagg ctaaacgaag ataagaggaa ttgtagtgat aaatcagcgt catcgtagtt
     7801 ttaggcccgc tgatcacagt tgtcattttt tcagcgagta aaacatatga ttccccatca
     7861 cgataatttg aagcaaaatc ccaggccgcg aaaaaacctg catcttcgtg gtatgcacgg
     7921 atctcactgt gcata
//
LOCUS       DAZPBM010000179         7875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPBM010000179 DAZPBM010000000
VERSION     DAZPBM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.180"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..268
                     /gene="rutC"
                     /locus_tag="ACHUZQ_003623"
     CDS             <1..268
                     /gene="rutC"
                     /locus_tag="ACHUZQ_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1027229.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
     gene            276..1076
                     /gene="rutD"
                     /locus_tag="ACHUZQ_003624"
     CDS             276..1076
                     /gene="rutD"
                     /locus_tag="ACHUZQ_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1027230.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            1086..1676
                     /locus_tag="ACHUZQ_003625"
     CDS             1086..1676
                     /locus_tag="ACHUZQ_003625"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1027231.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            1687..2181
                     /gene="rutF"
                     /locus_tag="ACHUZQ_003626"
     CDS             1687..2181
                     /gene="rutF"
                     /locus_tag="ACHUZQ_003626"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1027232.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            2202..3530
                     /gene="rutG"
                     /locus_tag="ACHUZQ_003627"
     CDS             2202..3530
                     /gene="rutG"
                     /locus_tag="ACHUZQ_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1027233.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3788..3961)
                     /locus_tag="ACHUZQ_003628"
     CDS             complement(3788..3961)
                     /locus_tag="ACHUZQ_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1027234.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHUZQ_003629"
     CDS             4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHUZQ_003629"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1027235.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            4951..5178
                     /locus_tag="ACHUZQ_003630"
     CDS             4951..5178
                     /locus_tag="ACHUZQ_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1027236.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHUZQ_003631"
     CDS             complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHUZQ_003631"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1027237.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(6751..7875)
                     /locus_tag="ACHUZQ_003632"
                     /pseudo
     CDS             complement(6751..7875)
                     /locus_tag="ACHUZQ_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1850 a         1961 c         1992 g         2072 t
ORIGIN      
        1 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
       61 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
      121 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
      181 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
      241 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
      301 cttatgctga tgcgcccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
      361 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
      421 gcaccggcaa taatcccgac acgctggcag aagattacag tatcgcccag atggcagcgg
      481 aactgcatca ggcgctggta gccgcaggga ttgagcatta cgcggtggtc ggccatgcgc
      541 tcggtgcgct ggtgggaatg cagctggcgc tggattatcc cgcgtcggta actgtgctga
      601 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
      661 aacgattact gtatagcggc ggcgcgcagg catgggtgga agcgcagccg ttgttcctct
      721 atcccgccga ctggatggcg gcccgcgcac cttgccttga ggcagaagac gcgctggcac
      781 tggcgcattt tcagggcaaa aataatttac tgcgtcgact taacgccctc aaacgcgctg
      841 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
      901 atctgctggt gccatcagca tgttccagtg agcttcatgc cgccctgccc gatagccaga
      961 aaatggtgat gcgctatggc ggacacgcct gcaacgtgac cgaccccgaa acgtttaatg
     1021 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     1081 ttgctatgaa cgaagccgtt agcccaggtg cgcttagcac cctgttcacc gatgcccgca
     1141 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacgggag ctttatgccc
     1201 cgataaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     1261 ctgtagaagg aaaagaacgt ctgcacccgg cgctttccag cggcaatcta caaaaaaccc
     1321 tgaccgcgcc cgtcaccgct atcgttgcct gggacagtga attttatgaa cggttaccac
     1381 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     1441 aaacagcgtt tcgcaacagc tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     1501 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     1561 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     1621 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     1681 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     1741 gcggtcaata tcatcaccac ggacggtcca tccgggcgcg ccgggttcac cgccagcgcc
     1801 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     1861 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     1921 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     1981 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     2041 tgccgcatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     2101 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     2161 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     2221 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     2281 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     2341 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     2401 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     2461 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     2521 cgaatatcag catcgcactg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     2581 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     2641 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     2701 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     2761 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggttta attgtcgcct
     2821 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     2881 tcgtcagcca tgctgcctgg ttcggtttgc cacatttttc cactcctgct tttaatagtc
     2941 aggcaatgat gctgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     3001 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     3061 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     3121 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     3181 ctgctgccgt catcgccatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     3241 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     3301 caggcgcaag gatctgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     3361 tggtcgcagt aaccttagtg ctgggcgcgg gtgattttgc tctcacgctg ggcggtttta
     3421 cgttgggagg gatcggtaca gccacctttg gcgcgatttt actcaatgcg ttgttaagta
     3481 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     3541 gccggggtta cccggcggtg cagattgttg acaacgtacg cattgttcat gccggatgcg
     3601 gcgtaaacgc cttatccggc ctacaaaaca gtgcaaattc aatatattgc aatcttctgg
     3661 taggcctgat aagcatcgcg catcaggcaa gcttacattt gtcatcagta tcaaccaccg
     3721 ggtaaccggc ggtgatggct ttttgacgag ctacagacaa aaatgcattc gtcattgatt
     3781 agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag
     3841 atgcgcgctg cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg
     3901 cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca
     3961 ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg
     4021 gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgcgggt agacccgcgt
     4081 ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa taattatcca
     4141 taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa
     4201 attacgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga
     4261 aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga
     4321 ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga
     4381 aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtc gatggcgcag aagttgtcgt
     4441 taagcgtgta ccggaaacca tgccgccgca attatttgaa aaagcaggcg gtaaaacgca
     4501 aactgcaccg gttgcaaccc cgcaagaact ggccgattac gacgccatta tttttggtac
     4561 acctacccgc tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg
     4621 cctgtgggct tccggcgcac tatacggaaa actggcgagc gtctttagtt ccaccggtac
     4681 tggcggcggt caggaacaaa ctattacttc aacctggacg acccttgcgc atcacggcat
     4741 ggtgattgtc cccattggct acgcagcgca ggaattattt gacgtttcac aggttcgcgg
     4801 cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc agccaagcca
     4861 ggaagaactg tctattgctc gttatcaagg ggaatatgtc gcaggtctgg cagttaaact
     4921 taacggctaa tcttcaacag gaggatacgc atgccaactc aagaagcgaa agctcatcac
     4981 gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa
     5041 gtcgccgggt acgacgaaaa aatggcagaa aagatttggg aagaaggtag cgatgaagtg
     5101 ttagtcaaag cctttgctaa ggccgataaa gattcgcttt tttggggcga acagaccatc
     5161 gaacgtaaaa acgtttaacc agcgactccc ccgcttctcg cgggggagtt ttctgttatt
     5221 tcaccgcttc attcaacacg ctatcaaatt tatccatcgg gcagaaaccg ttggcatcta
     5281 tcgggcaacc gcttaattcc agcgtcacac gctgagcagg tgcctgcagg gttaacgcat
     5341 cggcattacg taactgttcc gcactctgat acacatattc aattttcatc aaatcgcgat
     5401 tggctttgct gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt
     5461 tctggtcatg caactgatac ggtttgaaat ccagcgccgt taacagagag gcaatgttgg
     5521 agtcgtgccc aaccaacact gtaattttcg gtgcgctggt gcgatcggtg accagagctt
     5581 tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca
     5641 ggctgtcctg gtagccgttt ttaagcttcg acaacacctt ccactgctgg tcagatttga
     5701 tttctcccca ggccacctga tccatcggaa aaccttcgta atattgcaaa gtaaacgcat
     5761 ctaccagcga gttgccgact ttcagcggcc cggaaacacc tggttcttgt tgatacttgg
     5821 cgctaaaggt atttttgcca tccaccagcg aacactgttg tttctcttta caggcagggg
     5881 aatctttata gttaacgatt ttttccagta gctggtagct gtcggtaagc tggagtttgc
     5941 tgagctcttt ctccattgcc gccaccgcct gttcactgaa tgcggcggaa tcatcggtga
     6001 tcaccgggtt aaaggttggg tccatagtgc ccattttttc ctggtgatgc acaggaatat
     6061 cacacccggg gaatgcgccg gtaataaaga actgtgcggt cgcaacggta cgttgcagac
     6121 tattggcata ggcataaacg gtgtccggcg gcgggcattc ccccgatttc accatcccct
     6181 gctctgccag ccattcacgc atgtaatggc ccatatacac ttcgagcacg ccacctttgg
     6241 tggtgagttg cccaccgggg acgtcccatt ctggccattt attcggcgtc gactgctcca
     6301 gcacactgcc attgttcgcc agcggcgcac gtaagttatg gcggctcatc atgagcactt
     6361 gctgtagctg atagccttcc ggtacggttt gtgcctgagc atttgaagcg agtaaaacta
     6421 tccctgccac agttgcggcg attagcgttt tgttcattcc tggcacctct tttgttatca
     6481 atcattaaag agtgtgacag aaatatgact tttttccggg aacaggttcg caaaatggct
     6541 tgttgaacgc ctgtttttct atatatatct tcaaaaacag gcaaggtttg acgcaaacta
     6601 tgcctgatta gcccctgcct cgcgtacgcc gctgataatt gcacctgtgg ctaaacagaa
     6661 tatcagcggc gttactaata cagaggcaat agtaccaata ataagagaag tgaggctaat
     6721 aacaatggtt tttagcacat gcctttgtca ttaaattgca taacgagtta aaaaatcaat
     6781 ttgcgcattc ctttccttat tgtttaactc gtaagcctga gcaaaaagtt caggagggag
     6841 gtaaacgtca taatgattgc cagtgtttat taaataccca tactcaaggt cgtcatctgt
     6901 tgaaaaggat tcattcccta aggagattga tgccacattg ctgtaaacaa taaataatga
     6961 gcaaatgcta tctgaaagag cagtgaggaa atttttcacc tcattaacct tctgctctgg
     7021 gtcggtgtct aaaacaaggc gtgccttgtt atcattaaat ataaattcgg gtaaatgcag
     7081 tatgcatttt ttgtcattca agcaactatc aataaattct tttttaaaag acaaaaaact
     7141 ccatgataaa aaaccaccct gcccctgaat tttgttgtca gattgtagat taacgccaaa
     7201 taacgtcttt acatcttccc tgagattata gaatgccgta tcaacaagag cctcgatcgt
     7261 ttgtttatca acaattttaa taaactcttc gtatacttgc ttttgcgtct tatggccgcc
     7321 cagcgcccat gaatactcat gttcttttaa aaccagtatc taaatcagat attcccggcg
     7381 tatttatctg ttctggctgg atactcatat tttcatcatc taaacaatac tcagaaaaaa
     7441 tatcatcaca cgcagtaatg ttttcaatat attccataaa gccataccaa taataacctt
     7501 taaaatctgt gatagactct gaacctctgc ccggcaagga cactctaaca atatcaacat
     7561 tttgaacata ttgtatagta tatgtaatac ctcctgaaac ccctgaataa atttgtgtat
     7621 cattagtacg caatgttctc ataaataatg atagatcatt atcattctgc atttcctcaa
     7681 attctttttc tagtgattca cattccagac atggagtatc ctcttcattt actttacgtc
     7741 tgagttcatt gtataaccgc ataaaacaca ccagtgtgtt ttcaaaaagc gtgattgaat
     7801 atgtttgatt tttttcgtac tcatttttta tatcgttcca ggcctgtttc aaataattat
     7861 ttgcagtgca actaa
//
LOCUS       DAZPBM010000180         7847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPBM010000180 DAZPBM010000000
VERSION     DAZPBM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.181"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            3..1235
                     /locus_tag="ACHUZQ_003633"
     CDS             3..1235
                     /locus_tag="ACHUZQ_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1027238.1"
                     /translation="MNSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGIISALYFLI
                     TPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGKIALCSAF
                     IFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTA
                     GYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALLLI
                     VMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGA
                     RLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTR
                     GIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTI
                     ILLCAYFKAQSDQCLLDK"
     gene            complement(1305..2276)
                     /gene="rfaC"
                     /locus_tag="ACHUZQ_003634"
     CDS             complement(1305..2276)
                     /gene="rfaC"
                     /locus_tag="ACHUZQ_003634"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1027239.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(2280..3326)
                     /gene="rfaF"
                     /locus_tag="ACHUZQ_003635"
     CDS             complement(2280..3326)
                     /gene="rfaF"
                     /locus_tag="ACHUZQ_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1027240.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(3336..4268)
                     /gene="rfaD"
                     /locus_tag="ACHUZQ_003636"
     CDS             complement(3336..4268)
                     /gene="rfaD"
                     /locus_tag="ACHUZQ_003636"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1027241.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4482..5678
                     /gene="kbl"
                     /locus_tag="ACHUZQ_003637"
     CDS             4482..5678
                     /gene="kbl"
                     /locus_tag="ACHUZQ_003637"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1027242.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            5688..6713
                     /gene="tdh"
                     /locus_tag="ACHUZQ_003638"
     CDS             5688..6713
                     /gene="tdh"
                     /locus_tag="ACHUZQ_003638"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1027243.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6952..>7847
                     /gene="waaH"
                     /locus_tag="ACHUZQ_003639"
     CDS             6952..>7847
                     /gene="waaH"
                     /locus_tag="ACHUZQ_003639"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR1027244.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         1958 a         1856 c         1903 g         2130 t
ORIGIN      
        1 aaattaattc tggtataaag tctttaattc tcatattatg tattttttct ttgatgactt
       61 tgtctttatg ggatgatgtt gcaacaaagt ttcttcatgc agctggaatt atatctgcat
      121 tgtattttct aataacacca aaaaaaacaa taactaacaa tcctactttg ttaattttca
      181 tctcattatg tcttttggga atcataaata tcatctggta ttcacattat aaagtttcag
      241 gctctgttta taccaatgca tatcgtggcc caatggaaac tgggaaaatt gccttgtgta
      301 gtgcttttat tttcttggtt ctttttgcta aaaatgagtt gagaacaaaa ataaaatttg
      361 ggaaactaat tctgttcgca tccctggcaa cgcagttact tttttttgcg catgccatgt
      421 ggcaacattt ctatttaaac gttgaccgtg ttgcattatc agcttcccac gctacaacag
      481 caggctacat catccttttc ccttctttac tggcatcaat tctcatttta aaatccgact
      541 ttagacataa aacaacatta tatacaatta acttcatgct tagcttatgt gctgtcatag
      601 taactgaaac gcgtgcagcc atattagtgt ttccattctt tgcgttacta ttaatcgtaa
      661 tggatagtta tattaataag cgaattaatt ataagttata ttgttttatt gcgattgcat
      721 tattagcagg tgtattttct tttaaagata cattgcttat gagaatgaat gacttaaata
      781 acgatttagt taattattcg catgataaca ccagaacttc agtcggtgcc cgtctggcaa
      841 tgtatgaagt tggcttaaaa acatattctc caataggaca atcactggaa aaacgagcgg
      901 aaaaaataca tgaactagaa gaaaaagagc ctagattgag tggcgcttta ccctatatag
      961 attctcattt gcataacgat ctcatagata cgttatcaac gcgtggtatt cctggagttg
     1021 tattaacaat tttagccttt tcagcaatac tcatatatgc cttaagaact gctaaagaac
     1081 cttatatttt aatcttgctt ttttcactac tggtagtagg cctaagtgat gtaatactct
     1141 tttctaaacc ggttccgact gctgtgtttg tcaccataat attgctttgt gcttatttta
     1201 aagcacaatc agaccaatgt ttattagata aataatgaat atgtgaaata aaatcagcaa
     1261 ggaggctgat tttatttcaa aattatctat taaggggttg taatttaaat catagcagcg
     1321 ttttcttcaa tgaatcgctt aacagcattt gctgtcaatt gagacaactc attccccgga
     1381 gccctacaaa ccatctgatt cttcccatat ccaccaatta atcccggatc ggttggtcca
     1441 taaaccgtga tattgggtct atccagtgcc gccgttaaat ggcttaaccc cgtatccacc
     1501 gacactacaa atttagcccc ggccagcacg cgggcaacgc cttccagact catcttcggc
     1561 aatacttcaa cataagcaaa tccttccgcc agtcgtttcg cccgctcttc ctcatgcggc
     1621 gcgccccacg gaagtttaat ccgtattcct gaatcagcca gtaaaccaat caattctcgc
     1681 cagtgttctt ccggccagtg tttatcatca cgggtcgtcg catgaagaaa tacggcatat
     1741 tcgccagcat ctgtaggcag gttcgtcagg aaatgctgtg caatagcata atcgccctgg
     1801 gtttgcggtt tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg ttctacggcg
     1861 tgctgctgtt ttgcaatatg atgcttacga ttgtaaaaca gactggctaa aggttcgcga
     1921 gcggtttgcc agtccatgcc atgctttacg ccatgcgcca gacgcgtcac cagcgccgcg
     1981 ctttttacca gcccctgagc gtcgataact gcgtcatagt tctctgcttg tagcgcttca
     2041 cgaaacgctt tgcgttccgc ttttatgggg gccgagaacc aggctttacg ccagcgacgt
     2101 attgccacag gaataactcg ctcaacggcg gcgtgccagg aaggaatctg tgcgaaccct
     2161 tcttccacca cccagtcaaa cttaatccct gggattgcct gctgggcatc agtgagtgcg
     2221 ggcaacgtat ggagaacatc gcccatcgac gatgttttaa cgatcaaaac ccgcatccgt
     2281 caggcttcct cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg gggagtaatg
     2341 tcgattaagc tctggtgata accctccgca gcgtcacctt tacgcacttt gtgatagccg
     2401 gtaatcaggc ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc cgggctactc
     2461 ggaccataca gggcaaccag cggacgattg agcgccgccg caacgtgcat caggccagaa
     2521 tcgttagtga caatggcttt acaggctgca atcaggataa ccgcttgatc aagctgtgtt
     2581 tcccccgcca ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc aagaatctca
     2641 ttgcccgctt catgatcttt cgccgagcca aacagaacca cctgataacc ttcatcaatc
     2701 agctgctttg ccagttccgc atagtggtag tgtggccagc gttttgccgg accaaactcc
     2761 gcacccgggc aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg attacaggta
     2821 tatgattttt caccttcgct cacctgcaat tgcggccata acaacggttt tggtagatct
     2881 tgtgcggtac gcatcacgcc tttgtcatag gccagcgcga catagcgttc caccattagc
     2941 ggccaggctt ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg catctcgccg
     3001 cgccagccgg tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga tttgaaggag
     3061 ttgggtaaga cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc cagtttgcgg
     3121 cgttcgccga tttccagcgc tccgtgaccg agaggcatag gaatagcttc gttaacttcc
     3181 ggcatccgcg ataataatgg acggcaccat gccggtgcca tcacatcgat tatcgcctgg
     3241 ggatagcgcg cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc gccaacccaa
     3301 gacgggccga tcaccagtat tttcatgcag agctcttatg cgtcgcgatt cagccaggcc
     3361 atgtattccg ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc cgccgcgcgc
     3421 agatttgtca gatctgcctg agtgaacgcc tggtagcggc ctttcagctt atccgggaac
     3481 ggaatgtatt cgatctggcc tttcttgtga taagccagcg tagcatcagc tacagcctgg
     3541 aaggattccg cacgaccagt accgaggttg aagatgccgg aaacgccatt ttccaggaac
     3601 cacagattca catcagccac gtcgcccaca tagacgaagt cgcgtttgaa gttctcgcta
     3661 ccttcaaaca gcttcggtga ttcaccgttg ttaagctggg tgttgagatg gaaagcgacg
     3721 ctcgccatgc tgcctttatg gccttcacgc ggtccataaa cgttgaaata gcggaagcca
     3781 acaatctgcg agttagcttc aggcaggatt tggcgaacat attcatcaaa caggaatttt
     3841 gagtaaccgt agacgttcaa cggtttttcg tactcgcggg attcaataaa gtcggaggtg
     3901 cgtccgccgt aggtggctgc ggaagaggca tacaggaacg ggatttcgcg ctccagacag
     3961 tagtgcagca gctctttgga gtattgatag ttgttatcca tcatatactt gccatcccac
     4021 tcggtggtgg aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc gaactcttcg
     4081 ccagccataa tctggatcag gaagtcttcc ttatccatat agtccgcgat atccagatcc
     4141 accaggttca caaacttggt gccgtctttc aggttgtcca ccaccagaat atcggtgatg
     4201 cctttatcat tcagggcctt aacgatgttg ctgccgataa agcccgcgcc gccggtaacg
     4261 atgatcataa ctgtaacctt cgaattatgg agtcagagac aatctcagac acgaatactt
     4321 ctatcatatc actacacggc gttggcttca gccattcgcc aaaagtgaga ttgatggacg
     4381 ttatttatcc tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac ctataagttt
     4441 gggtaatatg tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga gaattttatc
     4501 agcagttaac caacgatctg gaaaccgcac gggcggaagg gttgtttaaa gaagagcgca
     4561 ttattacgtc tgcgcagcaa gcagatatca ctgtggctga tggaagccac gtcattaact
     4621 tttgtgccaa taactatctc gggctggcga attatccgga tctgattgcg gcggcaaagg
     4681 cgggaatgga ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc ggcactcagg
     4741 acagccataa agagcttgaa caaaaactgg cggcattcct ggggatggaa gatgcgattc
     4801 tctactcttc ctgctttgat gctaacggtg gcctgtttga aacgctgctg ggcgcggaag
     4861 acgccattat ctccgacgca ctgaaccacg cgtctattat tgatggtgtg cgtctgtgta
     4921 aagctaaacg ctatcgctat gccaacaacg atatgcagga gctggaagca cgtctgaaag
     4981 aagcgcgtga agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg ttctcgatgg
     5041 acggcgtgat tgccaacctg aagggcgttt gcgatctcgc agataaatat gatgccctgg
     5101 tgatggtaga cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt ggttcccatg
     5161 aatactgcga tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt aaagcgctgg
     5221 gcggggcttc tggtggttat accgcggcgc gcaaagaagt ggttgagtgg ctgcgccagc
     5281 gttctcgtcc gtacctgttt tccaactcgc tggcaccggc cattgttgcc gcatccatca
     5341 aagtactgga gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg gcgaacgcgc
     5401 gtcagttccg tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc gatcacgcca
     5461 ttattccggt catgcttggt gatgcggtag tggcgcagaa atttgcccgt gagctgcaaa
     5521 aagagggcat ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt caggcgcgta
     5581 ttcgtaccca gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca gtagaagcat
     5641 tcacgcgtat tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg aaagcgttat
     5701 ccaaactgaa agcggaagag ggcatctgga tgaccgacgt tcctgtaccg gaactcgggc
     5761 ataacgatct gctgattaaa atccgtaaaa cagccatctg cgggactgac gttcacatct
     5821 ataactggga tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg ggccatgaat
     5881 atgtcggtga agtggtaggt attggtcagg aagtgaaggg cttcaagatt ggcgatcgcg
     5941 tttctggcga aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc ggtcgtaccc
     6001 atctgtgccg caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc gccgaatatc
     6061 tggtgattcc ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac gacctggctt
     6121 ccatttttga tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat ctggttggcg
     6181 aggatgtgct ggtctctggt gcaggcccga ttggtattat ggcagcggcg gtggcgaaac
     6241 acgttggtgc acgcaatgtg gtgatcactg atgttaacga atatcgtctc gaactggcgc
     6301 gcaaaatggg tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat gatgtgatgg
     6361 cagaactggg catgaccgaa ggctttgatg tcggtctgga aatgtccggt gcgccgccag
     6421 cgtttcgtac catgctcgac accatgaacc acggcggtcg cattgcgatg ctgggtattc
     6481 caccgtccga tatgtctatc gactggacca aagtaatctt taaaggcttg ttcattaaag
     6541 gtatttacgg tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg attcagtcag
     6601 gtctggatct ctcgccgatc attacccatc gtttctctat cgatgatttc cagaagggct
     6661 ttgacgctat gcgttcgggc cagtccggga aagtaattct gagctgggat taacacgaac
     6721 aagggctggt attccagccc ttttgcctga ggataatccg ttaaatatgt aaaatcctgt
     6781 cagtgtaata aagatatcgt aattgcgctg atctcttata tagctgctct cattatctct
     6841 ctaccctgaa gtgactctct cacctgtaaa aataatatct cacaggctta atagtttctt
     6901 aatacaaagc ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac
     6961 agcaccaata aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact
     7021 tgtatggaat ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat
     7081 ggttcaacgg ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt
     7141 cgtttgttgc atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg gattgaagtg
     7201 gcaacgggca aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc caccatgtac
     7261 gaaacgctga tgaccatggc gttagaggac gacctcgacg tggcgcagtg caacgctgac
     7321 tggtgttttc gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg ccttcgctca
     7381 accggcgtat taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact
     7441 cacgttgtct ggatgggggt ttatcgccgc gatgttattg ttaaaaataa cattaaattt
     7501 attgccggat tacatcatca ggatattgtc tggacaacag aatttatgtt taatgcgctg
     7561 cgtgcgcgat ataccgagca atcgttatat aaatattatc tgcataatac gtcagtgagt
     7621 cggttgcata gacaagggaa caaaaacctt aattatcaac gtcactatat taagattact
     7681 cgtctgctag agaaattaaa tcgaaattat gccgacaaaa ttacgattta tccggaattt
     7741 catcagcaaa taacttacga agcattgcgt gtttgccatg cggtgcgcaa agagccggat
     7801 attattaccc gccaacggat gattgccgag atatttactt ccggtat
//
LOCUS       DAZPBM010000181         7777 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPBM010000181 DAZPBM010000000
VERSION     DAZPBM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.182"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHUZQ_003640"
     CDS             complement(<1..1780)
                     /locus_tag="ACHUZQ_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR1027245.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUZQ_003641"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUZQ_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1027246.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUZQ_003642"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUZQ_003642"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1027247.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUZQ_003643"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUZQ_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1027248.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUZQ_003644"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUZQ_003644"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1027249.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHUZQ_003645"
     CDS             complement(5968..7005)
                     /locus_tag="ACHUZQ_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1027250.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7777
                     /gene="yhhY"
                     /locus_tag="ACHUZQ_003646"
     CDS             7339..>7777
                     /gene="yhhY"
                     /locus_tag="ACHUZQ_003646"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1027251.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2103 c         1872 g         1825 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacgcat
//
LOCUS       DAZPBM010000182         7715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPBM010000182 DAZPBM010000000
VERSION     DAZPBM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.183"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHUZQ_003647"
     CDS             <1..643
                     /locus_tag="ACHUZQ_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1027252.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUZQ_003648"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUZQ_003648"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR1027253.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHUZQ_003649"
     CDS             2474..2986
                     /locus_tag="ACHUZQ_003649"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1027254.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUZQ_003650"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUZQ_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1027255.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHUZQ_003651"
     CDS             5579..5782
                     /locus_tag="ACHUZQ_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1027256.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUZQ_003652"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUZQ_003652"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1027257.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="ACHUZQ_003653"
     CDS             complement(6827..>7715)
                     /locus_tag="ACHUZQ_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR1027258.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1782 a         2103 c         2022 g         1808 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAZPBM010000183         7708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPBM010000183 DAZPBM010000000
VERSION     DAZPBM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.184"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUZQ_003654"
     CDS             complement(<1..304)
                     /locus_tag="ACHUZQ_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1027259.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="ACHUZQ_003655"
     CDS             complement(1125..1337)
                     /locus_tag="ACHUZQ_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFR1027260.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1413..2030)
                     /locus_tag="ACHUZQ_003656"
     CDS             complement(1413..2030)
                     /locus_tag="ACHUZQ_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR1027261.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2297..3796
                     /gene="ansP"
                     /locus_tag="ACHUZQ_003657"
     CDS             2297..3796
                     /gene="ansP"
                     /locus_tag="ACHUZQ_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR1027262.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3911..4972)
                     /locus_tag="ACHUZQ_003658"
     CDS             complement(3911..4972)
                     /locus_tag="ACHUZQ_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR1027263.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5214..7316
                     /gene="pqqU"
                     /locus_tag="ACHUZQ_003659"
     CDS             5214..7316
                     /gene="pqqU"
                     /locus_tag="ACHUZQ_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR1027264.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="ACHUZQ_003660"
     CDS             complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="ACHUZQ_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1027265.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
BASE COUNT         1962 a         1801 c         1857 g         2088 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttgtc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 ataataaata acataatcct ctggtatccc actcaaataa tttcattcct ttttaatact
     1441 tcttgtaatt tcggtaactg acatacagca tcagcaatcg cactgatatt cgtggcatta
     1501 tcctgaaacc attcatgccc agccccccat gtgcgcatag tgcaaagata acagtccacc
     1561 agcgttagtt gctcaccaaa cgcataaggt tcagcggtca attgtgaatt cagccagata
     1621 taaagcgatt tacgatattc aataacattc ttctttaact gttcaggtgc gtcaggagcc
     1681 caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac cagccagacc
     1741 aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag gtccgggcga
     1801 cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca taatctcgtc attttccagc
     1861 gccagtgtcg gaatctgaca caacgggttc agggttttca ataactcgcg tgaagttccc
     1921 tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag tgttagcatc
     1981 agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat atgcccctca
     2041 caatctttta aagattaagt gtaggacagt acacaggtta accagcaaaa gtatttgtca
     2101 ttttttcgtc agttgtatga atccccttca ggattgtaag gggatgtttt gcatcgtata
     2161 tcgataacgg gtaggatgct gcgccgatag ttttataccc ataaagaata atggtgataa
     2221 ctatcatcgc caggatgaat aaacattgtt catggcaact tatatgactt tttcattaaa
     2281 gcaatcaggg agagcaatga gtaaacacga caccgacact tcagatcaac acgccgcgaa
     2341 acgccgctgg cttaatgccc acgaagaggg gtatcacaaa gcgatgggca atcgccaggt
     2401 acagatgatc gccattggcg gcgcgattgg caccggcttg tttttaggtg caggagcccg
     2461 actgcaaatg gcgggtccag cactggcact ggtttattta atttgtggct tgttttcgtt
     2521 ttttattctg cgtgcattgg gtgagctggt gctacaccgc ccttccagtg gcagttttgt
     2581 ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat gttgctggct ggatgtactt
     2641 catcaactgg gcgatgacgg ggattgttga tattaccgcc gtcgctctgt atatgcatta
     2701 ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg ctcgctgcac ttaccatcgt
     2761 tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag atggagttct ggtttgcgct
     2821 tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt acagtgttcc tcggtagtgg
     2881 tcagccgctg gatggcaaca ccactggctt tcatttaatc accgataatg gcggcttctt
     2941 cccccacggt ttgctgcctg cgctggtgtt gattcagggc gtagtgtttg cttttgcctc
     3001 cattgaaatg gtgggtacag ctgccggaga atgtaaagat ccgcagacca tggtgcctaa
     3061 agccattaac agtgtgattt ggcgtattgg cctgttttac gtcggctccg tggtgttgct
     3121 ggttatgtta ttgccgtgga gcgcgtatca ggcggggcaa agtccgttcg tgacgttttt
     3181 ctctaaactg ggtatgccat atatcggcag cattatgaac attgtggtgc tgaccgctgc
     3241 cctctccagc ctgaactcag gtctgtactg caccgggcgt attctgcgct cgatggcgat
     3301 gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt cagcatgtgc cgtatgccgg
     3361 gattctggcg acactggttg tgtatgtcgt cggcgtattc ctcaactatc tggtgccgtc
     3421 gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga atcatcgctt catgggcgtt
     3481 tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa gaaggcaaag cagcggatgt
     3541 cagttttaaa ctgcctggcg cgcccttcac ttcctggctg acattactgt ttttactgag
     3601 tgtccttgtg ctgatggcgt tcgattaccc gaacgggact tatactatcg cggcgctgcc
     3661 gattatcggt attctgctgg ttataggctg gtttggtgtg cgcaaacgcg ttgctgaaat
     3721 tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa caggaaattg tgtttaagcc
     3781 tgaaacggcg agctaattgc aaaattgccc ggctacgtgc cgggcaaatt tagattactg
     3841 acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa gtctctccgt tttcaccctc
     3901 gccatatcta ttacagcgca atacgaatca catcgtctgg ctgggtagct tcctgctgtt
     3961 tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac agcgccaggc
     4021 tgtttggatg agtcggcgta tcgaacgttt tcactacttt atagcttttc gcgtcaatca
     4081 cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgct gggttaaaca
     4141 gcacagccag tgattccggt gctgcaacct tcgccagaat attgccatta cgggtatcga
     4201 ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgcctg gcggtatcaa
     4261 ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta cggctgagga
     4321 ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg gtgtaaagac
     4381 gtttgccttt gctatcaagc gccagaccgg tactcatttt accggtgttc tggatggcgg
     4441 ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta ccaataccac
     4501 tgatgtaaac ggtgttcgtg gcatcgtcag ctaccagctc acgcggttgc agcgggcgca
     4561 cctcttccgt acgcttacga tcatccagca ccagacggcc tttcacctcg cccgttttgg
     4621 catctatcgc cgtgaccgcg ctgtttacag tgttaccaaa ccacagtgtc tgagtcgtgt
     4681 tattgatggt gacaccaaac ggcttgagat cgttatggat cgcctgcgtc acttccagag
     4741 tgaccggatc aagacgataa accacgccgc ctttatccag tttgcggctt tgcgaagtgg
     4801 cgagccacag cgcgttttct tgctggctat aagccatttc gtaggcacct ttacctaccg
     4861 ctttacgcag catttcttct gcggcctgcg tactgaatga tgaagcaaca agcaatgaac
     4921 ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc atgacgactc
     4981 cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta atcatcgtta
     5041 cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag catttcttgc
     5101 cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt ttccatgaat
     5161 ttacaatttt attaccataa cggcgttttc ccattcttaa cctggctttt ttcatgaaga
     5221 ttttttccgt ccgacagacc gttttgcccg cactacttgc cctttccccc gttgtttttg
     5281 ccgctgatga acagaccatg attgtcagtg ccgcaccgca ggtggtttca gaactggata
     5341 tcccagcagc agtaagcgtg gtggatggcg aggagatgcg cctggcaaca ccgcgcatta
     5401 acttgtccga atcactgacc gacgtgcctg gtttgcaggt acaaaaccgg cagaactatg
     5461 cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac ggtattcgcg
     5521 gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacgggcaa gggcaaacat
     5581 ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct gcgtggcccc ttctctgccc
     5641 tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac ccagaccgga caacagccac
     5701 caaccattga agccagtagt tactacggca gttttggcag ctggcgctat gggctgaaag
     5761 caacgggcgc aacgggagac ggcacacagc ctggcgatgt cgattacacc gtctcaacca
     5821 cgcgttttac gacccacggc tatcgtgacc atagtggcgc acagaaaaat ttagccaatg
     5881 ccaaactggg cgtacgcatt gatgaagcca gcaaattaag tctgattttc aatagtgtgg
     5941 atatcaaagc agatgacccg ggtgggctaa ccaaagcaga atggaaggct aatccacaac
     6001 aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat caagcaaact caggctgggt
     6061 tgcgctatga gcgtagcctg agcgcgcaag atgatatgag tgtgatgatg tatgccggag
     6121 agcgagaaac gacccagtac cagtcaatac ccatggcacc acaacttaac ccgtcacatg
     6181 cgggcggcgt gattaccctg caacgccatt accaggggat agacagccgc tggacacacc
     6241 gtggtgaact gggcgttccg gtcacgttca ctaccggcct gaactacgaa aacatgagtg
     6301 aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg catgccggag tacgggcaaa
     6361 aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa catcgatccc tatttacaga
     6421 cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac agctccgtgt
     6481 ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc ggtgatgcca
     6541 gttatcataa atggctacct gccggttcgt taaaatatgc aatgaccgat gcctggaata
     6601 tctatctggc agccgggcga ggttttgaaa cgccgacgat taatgagctg tcttatcgtg
     6661 ctgatgggca aagcggtatg aactttggtt taaaaccatc caccaacgat acaattgaga
     6721 tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttt cagaccgaca
     6781 ctgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac aaaaatgccg
     6841 gaaagacccg tcgtcaaggc gctgaactgg catgggatca acgtttcgca ggagattttc
     6901 gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg cagcaatgtt tgcaatgaac
     6961 aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt gcgtcgatag
     7021 gttatgtacc ggaagatggt tggtatgcag gcacggaagc gcgttatatg ggcgatatta
     7081 tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac tctcgtcggc ttattcaccg
     7141 ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc gataatttat
     7201 tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga tattacgaac
     7261 cttcgcccgg acgaaattat ggtgtcggca tgaatattgc gtggagattt gagtaattac
     7321 cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg tataaaatag
     7381 caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg tcttttgctt
     7441 ttaacgcagc gagtaacaga tgatagtttt ctatatgctc ccgtaattcc gctggattaa
     7501 tcgcttcata gagataatgt aaaccaggcc ccatcctgac ccacagttgc tcaatcatct
     7561 cacacaggat gggcatattt gagcgatgat aaatcgctaa gcgaaatagc ctgtttacat
     7621 taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg agcaattcct
     7681 ggagttccgt caggtcttgc ggggtgag
//
LOCUS       DAZPBM010000184         7611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPBM010000184 DAZPBM010000000
VERSION     DAZPBM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.185"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            171..701
                     /locus_tag="ACHUZQ_003661"
     CDS             171..701
                     /locus_tag="ACHUZQ_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1027266.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            837..1502
                     /locus_tag="ACHUZQ_003662"
     CDS             837..1502
                     /locus_tag="ACHUZQ_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027267.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1824..2447
                     /locus_tag="ACHUZQ_003663"
     CDS             1824..2447
                     /locus_tag="ACHUZQ_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR1027268.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="ACHUZQ_003664"
     CDS             2532..2750
                     /locus_tag="ACHUZQ_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1027269.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="ACHUZQ_003665"
     CDS             2728..3798
                     /locus_tag="ACHUZQ_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1027270.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="ACHUZQ_003666"
     CDS             3933..5216
                     /locus_tag="ACHUZQ_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1027271.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="ACHUZQ_003667"
     CDS             complement(5450..>7611)
                     /locus_tag="ACHUZQ_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1027272.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAZPBM010000185         7561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPBM010000185 DAZPBM010000000
VERSION     DAZPBM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.186"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(32..408)
                     /gene="rnpB"
                     /locus_tag="ACHUZQ_003668"
     ncRNA           complement(32..408)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUZQ_003668"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(441..1586)
                     /gene="garK"
                     /locus_tag="ACHUZQ_003669"
     CDS             complement(441..1586)
                     /gene="garK"
                     /locus_tag="ACHUZQ_003669"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1027273.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1683..2573)
                     /gene="garR"
                     /locus_tag="ACHUZQ_003670"
     CDS             complement(1683..2573)
                     /gene="garR"
                     /locus_tag="ACHUZQ_003670"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1027274.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2603..3373)
                     /gene="garL"
                     /locus_tag="ACHUZQ_003671"
     CDS             complement(2603..3373)
                     /gene="garL"
                     /locus_tag="ACHUZQ_003671"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR1027275.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3389..4723)
                     /gene="garP"
                     /locus_tag="ACHUZQ_003672"
     CDS             complement(3389..4723)
                     /gene="garP"
                     /locus_tag="ACHUZQ_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1027276.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5098..6669
                     /gene="garD"
                     /locus_tag="ACHUZQ_003673"
     CDS             5098..6669
                     /gene="garD"
                     /locus_tag="ACHUZQ_003673"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1027277.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6818..7230
                     /locus_tag="ACHUZQ_003674"
                     /pseudo
     CDS             6818..7230
                     /locus_tag="ACHUZQ_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            7230..>7561
                     /gene="yhaV"
                     /locus_tag="ACHUZQ_003675"
     CDS             7230..>7561
                     /gene="yhaV"
                     /locus_tag="ACHUZQ_003675"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1027278.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT         1906 a         2028 c         1842 g         1785 t
ORIGIN      
        1 aaacccgccg aagcgggttt ttacgtaaat caggtgaaac tgaccgataa gccgggttct
       61 gtcgtggaca gtcattcatc taggccagca atcgctcact ggctcaagca gcctacccgg
      121 gttcagtacg ggccgtacct tatgaacccc tatttggcct tgctccgggt ggagtttacc
      181 gtgccacgga ctgttaccag ccgcgcggtg cgctcttacc gcaccctttc acccttacct
      241 gatcccgctt gcgcgggcca tcggcggttt gctctctgtt gcactggtcg tgggtttccc
      301 ccccaggcgt tacctggcac cctgccctat ggagcccgga ctttcctccc ctccgcccgt
      361 ctcccccgaa gaggacgacg acgaagcggc gactgtctgg tcagcttcgg cgcgcagtat
      421 agagggtttg cgcgcccttg tcaccccgcg ttgcgcattc caatcgccag tgtcgcggcg
      481 atattacgtg aagcacggca gatattgtca taagccccgc ggaatgcttc gtccaacgta
      541 cctatgctgg tcaatacgct gaagaccgca tcgatgccat gctgatgtac aacgccaaca
      601 tcatcggtca ggctacccgc aatgccaatc accggtttat ggtacttctt cgccacgttt
      661 gcaacgccaa tcggtacctt gccgtgaata ctctggctgt caatacgccc ttcaccggtg
      721 atcaccagcg tacaatcgtg aatatgttct tccagattca gcgccgtagt gacgatttca
      781 ataccacttt tcagttccgc accaagaaac gccatcagcg ccgcgcccat accgcccgca
      841 gctcctgcgc cggggacatc tttcacatca acatgcagcg cttttttaat gacatcggca
      901 tagtgagaga ggttattgtc cagctcaaca atcatcgctt cactggctcc cttttgtggg
      961 ccaaagatgc gcgatgcgcc gttatcgccc accagcggat tggtgacatc acaagcgacg
     1021 cgaatgacgc aatcttttaa gcgcggatcg aggccggaaa tatcaatatc attcagagta
     1081 ttaagactac cgccgccaaa accaatttca ttgccgttgg cgtcgcataa tttcgccccc
     1141 agcgcctgta ccatgcctgc gccgccatca tttgtagcgc tgccgccaat gccgataata
     1201 atgtttgttg cactgctctc cagcgcctgc aggattaact cgcctgtgcc gcgtgaagtg
     1261 gttacgagtg gatcgcgttt ttccgcaggt accagctcca gcccactggc cgccgccatt
     1321 tcaataaacg cggttttgcc atcgccggag atcccccaac tggcattcac tttctcgccc
     1381 agcggccctg taacccaggc gtgacgttca gccccctggg tggctgcaat catcgcttcc
     1441 accgttcctt caccaccgtc ggcaaccgga acagaaacat actgtgcatc aggaaaaatt
     1501 tcccgaaatc ctttttctat cgcctgcgca acctcgctgg cagataaact ttctttataa
     1561 gagtctgggg cgattacgat tttcatacct atgcctgtta ccacatgacg ccggagggcg
     1621 tttctcttat tcggcctaga ttccaggccc ggattgcaat acaccatccg ggcacgacgt
     1681 cattaacgag taacttcgac tttcgccagt ttttcgtagt agcacgccag ggcgctatga
     1741 tccgccgttc ctaaaccatc tgcacgcagt gcctgcatca tctccataac cgcagctgtg
     1801 agcagcagtt gtgcgccgac gccgtgagaa gtatccagcg cattcgccag atccttaata
     1861 tgcagatcaa tgcggaagcc cggcttgaag ttgcggtcca tcaccatcgg cgctttggca
     1921 tccagcacgg tactgcccgc cagcccaccg cgaattgcct gataaaccag gtccgggtta
     1981 acgcccgctt tggttgccag cgttaacgct tctgacatcg cagcaatatt cagcgccaca
     2041 atgacctgat ttgccagttt ggtgacgtta cctgcaccga tttccccggt atgcaccacg
     2101 gaacccgcca tcgctttcat caaatcatag tatttgtcga aaatagcctt gtcgccgccc
     2161 accatcactg acagcgtacc gtcgatggct ttcggttcac cgccgctcac cggagcatcc
     2221 agcatatcaa tgcctttcgc tttcagcgct tcgctgattt cacggcttgc cagcggtgcg
     2281 atagaactca tatcgatcaa taccgtacct ggcttcgcgc cttcaataat gccattctca
     2341 cccagcgcca cctctttcac atgaggggag tttggcagca tggttatgat gacgtcgcac
     2401 tgttcagcga tcgctttagc cgtagacgct gtttctgcac ctgcagcaat cacgtcagca
     2461 atagcttctg ggttacggtc agcaaccacc agcgagtaac ctgctttcag aaggttttta
     2521 ctcattggtt tacccataat ccccaggcca ataaaaccaa ctttcatagt catatcaacc
     2581 atctctcttg ttgcggtggt ggttattttt taaaggtatc agccagtttc tgagtggcag
     2641 agcggaagac gccaagatcg ctgccgacag ccacaaacgt cgcgccccat tccagataac
     2701 gacgcgcatc ggcttcgacc ggcgcgagaa taccgctggg tttgccgtgc gcgctggcac
     2761 ggttaaaaat atgctgaatt gctttttgta catccggatg cgaggcattg ccgagatggc
     2821 ccaatgccgc agccagatcg ctggggccga cgaagatacc gtctacacct tcggtagcgg
     2881 caatagcatc gacgttatct acgccctgct gactttctat ctggaccaga atagtaatgt
     2941 tcttgttcga ctgagcgaaa taatccgcca cggtgccaaa catattggcg cggtgagaaa
     3001 cggagacgcc gcgaatgcct tccggcgggt aacgagttga tgccaccgcc tgctctgctt
     3061 cctcttttgt ttctacaaaa ggaatcagga agttatagaa accgatatcc agaagacgct
     3121 taataattac cggctcgttg gtcggcactc gcactactgg cgcgctggcg ctgcctttca
     3181 gggccattaa ctgcggaata aacgtggaaa tatcgtttgg cgcatgttcg ccatccagca
     3241 ccagccagtc aaacccagcc aaaccaagaa cttcagtgct aatcgggtta gagagtgctg
     3301 accagcaacc aatttgtacc tgtttcgcag ccagtgcggc tttgaattta ttcgggaaaa
     3361 catcgttatt catcgcttat acctttgctt atttctgcaa ttccatacgt ttaatgtcgc
     3421 caactacgaa gaggtagcag accatcgcca tcagcgctga acatcccacg aaaaccagtg
     3481 ctgcattgaa ggagtgcagt tcacttacca ggtagccaat caccagtgga gtgacaatgg
     3541 aggcaacatt gccaaagacg ttaaagacgc cgccacagag gccaacaatc tctttcggcg
     3601 cggtgtcaga aatcaccggc cagcccagag caccaaatcc tttgccaaaa aaagccagcg
     3661 ccatcagcat gaccaccagc gtggtgttgt tggtgtagtt acataagatg atggtggaaa
     3721 ccagcaacat tcccagcaca atcggtagct tacgtgccag ggtcagggat aaaccgcgtt
     3781 tgatcagata atccgagaag acacctccca gcacgccgcc cgcaaaacca cacagtgccg
     3841 gaatcgaggc gaccagaccc actttcagaa tcgacatgcc tttttcctgc accagataaa
     3901 tcgggaacca ggtgaggaag aaccaggtga tggtgttgat aaaatattgt ccgaaaaata
     3961 cgcccaacat catgcggtta gagagcaatt gcttgatgta atgcagtttg ggaccgcttg
     4021 ctgccgcgct gcccggcttt ttgtggtcca tatcgaccac cgcgccattt tcagagataa
     4081 acttcagctc ttccgcagac atacgtgggt gatctgtcgg gttatgaatc aacttgatcc
     4141 acagcgccgt cagcacaaaa ccaatccccc ccataacggt gaagacgtgc tcccagcccc
     4201 aagcgaaagt cagccagcca agcagcggcg aaaagagcgc cagcgagaaa tattgcgccg
     4261 agttaaagat ggcggaggca gtaccacgtt ctttcgtcgg gaaccaggcg gcgacaatac
     4321 gggcgttcgc cgggaatgat ggcgcttccg agaaccccag cataaagcgc ataaagaaca
     4381 tggagatccc tgcccaggcc agcgggaaca tatcaacaaa gccttgcagg aaggtgaaca
     4441 gcgaccagaa aaagaggctg taggtgtaaa cttttttcga gccaaactta tcaagcaacc
     4501 agccgccggg gatttgcatc agcaagtagg cccagccaaa agcagagaag atgtaaccca
     4561 tcgaaaccgc acttaactgc aactcttttg ccacttcggt accagcaata gacagcgttg
     4621 cacgatcggc gtagttaacg gcggtaacaa taaaaataat cagtaatatt aaatagctgg
     4681 tatgcacgcc tttctttttt acgtcaacgg tgtccagaat cattttattt acctcgggta
     4741 cttatgctga tttttattat tatggggaag gtgttattta tgagttttat ttatgccgtt
     4801 acgacaatga actcgggaat tagtataagc agcgggagaa taataatcat tgtgcaaatg
     4861 ctaatttaat cagtactatt taaatattat tttgagcata tgcacataag gttgcgcgct
     4921 aaagcacaga tttgcgcttt accttaccgg gcggcactgc aatccctgaa atgattgaca
     4981 ttgatcacat ttctgcgttt aaactcctga cattcttatt tcacccaatg aagtcattta
     5041 tttttaaatg agaccagatc ctcattttaa taacccctgg ctggagaata ttgcacaatg
     5101 gccaacatcg aaatcagaca agaaacgcca actgcgtttt atataaaagt tcacgacaca
     5161 gataatgtgg caattattgt taatgataat ggcctgaaag caggaacgcg ttttccggat
     5221 gggctggaat taattgaaca tattccccag gggcataaag tcgcattgct ggacattcca
     5281 gctaatggtg aaattattcg ttatggcgaa gtgattggtt acgccgtgcg cgcaatccca
     5341 cgcggaagct ggatcgacga atcaatggtt gtactaccgg aagcgccgcc gttacacacg
     5401 ctgccactgg caaccaaagt cccggccccc ttaccgccgc tggaaggata cacctttgag
     5461 ggctatcgca atgccgatgg cagcgtgggc accaaaaacc tgctcggcat caccaccagc
     5521 gtccactgtg tggcaggcgt ggtggactat gtagtaaaaa tcattgaacg cgatctgcta
     5581 ccgaaatacc cgaacgtcga tggcgtggtg gggctgaatc atttgtacgg ctgtggcgtg
     5641 gcgattaacg caccggcggc agttgtgcct attcgtacca tccacaatat ttcgctgaac
     5701 cctaactttg gcggcgaagt aatggtgatt ggtctgggtt gtgaaaagtt gcagcctgag
     5761 cgcctgctgg tcggcacgga tgatgtgcaa gctattccag tagaaagcgc cagcattgtc
     5821 agtttgcagg atgaaaagca tgtcggcttt cagtccatgg tcgaggatat tttgcaggtc
     5881 gccgaacgcc atctacaaaa actgaatcaa cggcagcgag aaacctgccc ggcttcagaa
     5941 ctggtcgttg gtgtgcagtg cggtggcagc gatgcgtttt ctggtgtaac ggcaaacccg
     6001 gcggttggct atgcgtctga tctactggtg cgctgcggcg caacggtgat gttttcagaa
     6061 gtaacggaag tgcgtgacgc gatccatctg ctgacaccac gcgcagtgaa cgaagaggtc
     6121 ggcaaacggc tgctggagga gatggagtgg tacgataact atctcaatat gggaaaaacc
     6181 gaccgcagcg ccaacccttc gccgggcaac aagaaaggcg gtctggcaaa tgtggtggag
     6241 aaggcactcg gctccattgc taaatcgggt aaaagtgcaa ttgttgaagt gctgtcgccc
     6301 ggtcaacgcc cgactaaacg cggattaatt tacgccgcga cgccagccag cgattttgtc
     6361 tgtggcacgc aacaggtggc ttcgggtatc accgtacaag tgtttacgac cggccgtggt
     6421 acgccgtacg gcctgatggc ggtacccgta attaaaatgg caacccgcac cgagctggcg
     6481 aaccgctggt ttgatttaat ggatattaac gcgggcacta tcgctaccgg cgaagagagc
     6541 attgaagagg tgggctggaa gttgttccac tttattctcg acgtcgccag cgggaagaag
     6601 aaaaccttct cggatcaatg ggggctgcat aaccagctgg cggtgtttaa cccggcaccg
     6661 gtgacctgat ttcttttgga acgtccctcg caaaacatgg ccttagtgcc atgtttttat
     6721 tgtttaaagc ccccacgtcc attaataatg catttgcatt accttagttc aagcttataa
     6781 tttgagcaga aaacaggctg taaaaggaca atgaatcatg cccgctaatg ctcgctctca
     6841 cgctgtactg accactgaat caaaggtcac gatacgcgga caaacaacta tccccgcgcc
     6901 agtgcgtgag gccttaaaac tgaagccagg ccaggacagc attcattacg aaattctgcc
     6961 tggtgggcaa gtatttatgt gccgactggg agatgaacag gaggatcata ctatgaatgc
     7021 atttttgcgt tttctggata cagatatcca gaacaacccg caaaaaactc gtccattcaa
     7081 cattcaacat tcaacattca acattcaaca ttcaacattc aacattcaac attcaacatt
     7141 caacattcaa cattcaacat tcaacaagga aagaaacttg tcgctggcat ggacgtcaac
     7201 attgatgatg agattggcga cgacgaataa tggattttcc acaaagggtt aatggttggg
     7261 cgctatatgc tcatccctgt tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga
     7321 cattaaaggg aaaagatcct gaaaattatc agagaaaagc cgccacaaag ttattggcgg
     7381 tagtccataa agtgattgag gagcatatca cggtcaatcc atcatcaccg gcattccgtc
     7441 atggcaagtc gttaggctct gggaaaaata aagactggtc acgggtaaaa tttggtgcag
     7501 gtcgttatcg tctcttcttt cgttatagtg aaaaagagaa agtcatcatt ctgggatgga
     7561 t
//
LOCUS       DAZPBM010000186         7491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPBM010000186 DAZPBM010000000
VERSION     DAZPBM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.187"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUZQ_003676"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUZQ_003676"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR1027279.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHUZQ_003677"
     tRNA            647..722
                     /locus_tag="ACHUZQ_003677"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUZQ_003678"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUZQ_003678"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR1027280.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUZQ_003679"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUZQ_003679"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1027281.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUZQ_003680"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUZQ_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR1027282.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUZQ_003681"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUZQ_003681"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1027283.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHUZQ_003682"
     CDS             complement(5917..6249)
                     /locus_tag="ACHUZQ_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR1027284.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUZQ_003683"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUZQ_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1027285.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1823 a         2031 c         1869 g         1768 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZPBM010000187         7424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPBM010000187 DAZPBM010000000
VERSION     DAZPBM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.188"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHUZQ_003684"
     CDS             complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHUZQ_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR1027286.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2013..5975
                     /gene="putA"
                     /locus_tag="ACHUZQ_003685"
     CDS             2013..5975
                     /gene="putA"
                     /locus_tag="ACHUZQ_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1027287.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHUZQ_003686"
     CDS             complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHUZQ_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1027288.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6941..>7424
                     /locus_tag="ACHUZQ_003687"
     CDS             6941..>7424
                     /locus_tag="ACHUZQ_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1027289.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1775 a         2057 c         1942 g         1650 t
ORIGIN      
        1 ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca
       61 aaaccgccgc aggctaagtc ccttagtttt cctgcaaccg tgacggcgga gccgaatgat
      121 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca
      181 aactaaacac tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc
      241 ccagccagcc gaactgcttc cagacgataa ctgtcagcgc accgatgatc atccccgcca
      301 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg
      361 ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg
      421 ccgccagcgc aatcgccacc agcgccacca ccagcaccat cacacgtcct acccacacca
      481 gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac
      541 tggagcacac cagcagctgg caacttaagg ttgacattac cgccgccaga atcgccgaca
      601 gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgtt
      661 cagcgttctg gtttaccgca cctgctaccg ccggatgctc gttaaagtaa gcgataccaa
      721 agaagccaac agccaccgcc cctgccaggc aaaggatcat ccaggtcata ctgatacgac
      781 gcgcatggac aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct
      841 gcccgaagta acccagcccc caacccatca atgagataat ggcaacaaag ttcagccctt
      901 tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc
      961 caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     1021 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     1081 tcgacgcagc tcccgccaac agagccgttt cgtaactcat gccaaaggta ctttcaaaca
     1141 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca
     1201 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg
     1261 gcaatgttaa ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     1321 tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg
     1381 aaagaaaaac cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg
     1441 ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt
     1501 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     1561 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     1621 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     1681 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     1741 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     1801 tgcggttgca cctttcaaaa aggttaactg ccgcagagaa aaagtctgag ttatttttta
     1861 atcactgtca tatcgatttc ttttattaac atttcattca tttttaagct tgctacgcaa
     1921 gtcacattta acatggttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa
     1981 gttgcacctt tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc
     2041 tggacgacgc gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacaccac
     2101 actggttaat taagcaggcg attttttctt atctcgaaca actggaaaac agcgatactc
     2161 tgccggagct acctgcgctg ctttctggcg cggccaatga gagcgatgaa gcaccgactc
     2221 cggcagagga accacaccag ccattcctcg actttgccga gcaaatattg ccccagtcgg
     2281 tttcccgcgc cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg gtttctatgc
     2341 tgctggaaca agcccgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc
     2401 aactggccga taaactgcgt aatcaaaaaa atgccagtgg tcgtgcagga atggtccaag
     2461 ggttattgca ggagttttcg ctgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag
     2521 aagcgttgtt gcgtattccc gacaaagcca cccgcgacgc gttaattcgc gacaaaatca
     2581 gcaacggtaa ctggcagtca cacattggtc gtagcccgtc actgtttgtt aatgccgcta
     2641 cctgggggct gctgtttact ggcaaactgg tttccaccca taacgaagcc agcctctccc
     2701 gctcgctgaa ccgcattatc ggtaaaagcg gtgaaccgct gatccgcaaa ggtgtggata
     2761 tggcgatgcg cctgatgggc gagcagttcg tcacgggtga aaccatcgcg gaagcgttag
     2821 ccaatgcccg caagctggaa gagaaaggtt tccgttactc ttacgatatg ctgggcgaag
     2881 ctgcactgac tgccgcagac gcacaggcgt atatggtttc ctatcagcag gcgattcacg
     2941 ccattggtaa agcgtctaac ggtcgtggca tctatgaagg tccgggcatt tcaatcaaac
     3001 tgtcggcgct gcatccgcgc tacagccgcg cccagtatga ccgggtaatg gaagagcttt
     3061 acccgcgtct gaaatcactc accctgctgg cgcgtcagta tgatattggt attaacatcg
     3121 atgccgaaga ggccgatcgt ctggagatct ccctcgatct gctggaaaat ctctgtttcg
     3181 agccggaact ggcaggctgg aacggcatcg gttttgttat tcaggcttat caaaaacgct
     3241 gcccgttggt gatcgattac ctggttgatc tcgccacccg cagccgtcgc cgtctgatga
     3301 ttcgcctggt gaaaggcgcg tactgggata gtgaaatcaa gcgtgcgcag atggacggcc
     3361 ttgaaggcta tccggtttat acccgcaagg tgtataccga cgtttcttat ctcgcctgtg
     3421 cgaaaaagct gctggcggtg ccgaatctaa tctacccgca gttcgcgacg cacaacgccc
     3481 atacgctggc agcgatttat caactggcag gtcagaacta ctatccgggt cagtacgagt
     3541 tccagtgcct gcatggtatg ggcgagccac tgtacgagca ggtcaccggg aaagttgccg
     3601 acggtaaact taaccgaccg tgtcgtattt atgccccggt tggcacacat gaaacgctgc
     3661 tggcgtatct ggtgcgtcgc ctgctggaaa acggtgctaa cacctcgttt gttaaccgta
     3721 ttgccgacac ctctttgcca ctggatgaac tggtcgccga tccggtcact gctgtagaaa
     3781 aactggcgca acaggaaggg caaactggat taccgcatcc gaaaattccc ctgccgcgcg
     3841 atctttacgg tcacgggcgc gacaactcgg cagggctgga tctcgctaac gaacaccgtc
     3901 tggcctcgct ctcctctgcc ctgctcaata gtgcactgca aaaatggcag gccttgccaa
     3961 tgctggaaca accggtagcg gcaggtgaga tgtcgcccgt tattaaccct gcggaaccga
     4021 aagatattgt gggctttgtg cgtgaagcca cgccacgtga agtagaacag gcgctggaaa
     4081 gtgcggttaa taacgcgcca atctggtttg ccacgcctcc ggctgaacgc gcggcgattt
     4141 tgcatcgcgc tgccgtgcta atggaaagcc agatgcagca actgattggt attctggtgc
     4201 gtgaagccgg aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg gtcgattttc
     4261 tccactacta cgccggacag gtgcgggatg atttcgctaa cgaaacccac cgtccattag
     4321 ggcctgtggt gtgtatcagt ccgtggaact tcccgctggc tattttcacc gggcagatcg
     4381 ctgccgcact ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa acgccgctga
     4441 ttgccgcgca agggatcgcc attttgctgg aagcgggtgt accccctggc gtggtgcaat
     4501 tgctgccagg tcagggggaa accgtgggcg cgcaactgac gggtgatgat cgcgtgcgcg
     4561 gggtgatgtt taccggttca accgaagtcg ctacgttact gcagcgcaat atcgccagcc
     4621 gcctggacgc tcagggtcgc cctattccgc tcatcgctga aaccggcggc atgaacgcga
     4681 tgattgtcga ttcttcagca ctgaccgaac aagtcgtcgt ggatgtgctg gcctcggcgt
     4741 tcgacagtgc gggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa gatgagattg
     4801 ccgaccacac gttgaaaatg ctgcgcggtg caatggccga atgccggatg ggtaatccgg
     4861 gtcgcctgac caccgatatc ggtccggtga ttgatagcga agcgaaagcc aatattgagc
     4921 gccatattca gaccatgcgt agcaaaggcc gtccggtgtt ccaggcggtg cgggaaaaca
     4981 gcgaagatgc ccgtgaatgg caaagcggca cctttgtcgc cccgacgctg atcgaactgg
     5041 atgactttgc cgaattgcac aaagaggtct ttggtccggt gctgcatgtg gtgcgttaca
     5101 accgtaacca gctaccagag ctgatcgagc agattaacgc ttccggctat ggtctgacgc
     5161 ttggtgtcca tacgcgtatt gatgaaacca tcgcccaggt cactggctcg gcccatgttg
     5221 gtaacctgta tgttaaccgt aatatggtgg gcgcagtggt tggtgtgcag ccgttcggcg
     5281 gcgaagggtt gtccggtacc gggccgaaag caggcggtcc gctctatctc taccgtctgc
     5341 tggcgaatcg cccggaaagt gcgctggcag tgacgctcgc gcgtcaggat gcagagtatc
     5401 cggtcgatgc acagttgaaa gccgcattga ctcagccgct aaatgcactg cgggaatggg
     5461 cagcaaatcg tccagaattg caggcgttat gtacgcaata tggcgagctg gcgcaggcag
     5521 gaacacaacg attgctgccg gggccgacgg gtgaacgcaa cacctggacg ctgctgccgc
     5581 gtgagcgcgt gttgtgtatt gccgatgatg agcaggatgc gctgactcag ctcgccgccg
     5641 tgctggcggt gggcagccag gtactgtggc cggatgacgc gctgcatcgt cagctagtaa
     5701 aggcattgcc atcggcagtc agcgaacgta ttcaactggc gaaagcggaa aatataaccg
     5761 ctcaaccctt tgatgcggtg atcttccacg gtgattcgga tcagcttcgc gcattgtgtg
     5821 aagccgttgc tgcgcgggat ggcgcaattg tttcggtgca gggttttgcc cgtggcgaaa
     5881 gcaatatcct tctggaacgg ctgtatatcg aacgttcgct gagtgtgaat accgctgccg
     5941 ctggcggtaa cgccagttta atgacgatcg gttaaacagg tagccggagg atgttacaac
     6001 ctcctccggc atctttaacg tggtcgaatc ccctcaataa taatccgctg cacgttttca
     6061 accgtttgat tgaaaaatat ctcatcgcgc aacgtcgcgc ctgtcaccgc ctccacctga
     6121 ggggcgaaat cggcgtaatg ttgagtggaa gcccaaatca tgaaaatcaa atgctgcgga
     6181 tcaatcggcg cgagtttgcc gcttttgacc caaccggcaa tcagcgcaga tttctcatca
     6241 attaatgcct tcaaatcgcc cgtcagttca tccattaaca gcggcgcgcc tgccagcatc
     6301 tccatacaga acaggcgcga agcctgcgga taatcgcggg agacttccag cttcagacgg
     6361 atgtactctt ttatcgccgc cagcggagcg aaatcttcac gaaacgcttt taacggtgcc
     6421 agccagatat cgagaatctg ccgcagcacg gcaatataca gcgcctcttt tgacggaaag
     6481 taatacagca gattggtttt tgaaacaccc gccaactctg cgatctgctc cagccttgtg
     6541 ccgtgaaaac cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc
     6601 gcgcttactg cgcgcgaacg tttacccgtt gttttcactg cgccttgcgt catgcgctct
     6661 cccctcttgt agattggcaa caggatagca aaacccccgc ccggcctcga ccttctgcac
     6721 tctcatcgcg cacagatgca tgttttatgt gcaactgttt tgaccgttta gtccactttt
     6781 tatcagatat tttagataac gtaatattaa catattaata acattaactt tttaaaaact
     6841 ggcatccgct ttgcaaacaa gccaatacgc ggctgaaaag gatatgcagg atgcagcgcc
     6901 ccgtctgact ttcactttac gagacgaaga gaggttgatg atgaaaattg gcgtattcgt
     6961 acctattggc aacaacggct ggctcatttc gacccacgcg ccgcagtaca tgccgacctt
     7021 tgaactgaat aaagccattg tgcagaaagc ggagcattac catttcgatt tcgccctgtc
     7081 gatgatcaaa ctgcgtggct ttggcggcaa aactgagttc tgggatcaca accttgagtc
     7141 gttcaccttg atggcgggtc tggcagccgt gacctcgcgc attcagattt acgccactgc
     7201 tgccacctta acgttacctc cggcaatcgt cgcccgtatg gccgcaacca tcgactccat
     7261 ctctggcggg cgttttggcg tcaacctcgt gactggctgg caaaagcccg tgtatgagca
     7321 gatgggtatc tggcctggcg atgactattt ctcccgtcgt tacgactatc tcaccgaata
     7381 tgttcaggtg ctgcgcgacc tgtggggcac ggggaaaagc gatt
//
LOCUS       DAZPBM010000188         7356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPBM010000188 DAZPBM010000000
VERSION     DAZPBM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.189"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            86..421
                     /locus_tag="ACHUZQ_003688"
                     /pseudo
     CDS             86..421
                     /locus_tag="ACHUZQ_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            418..873
                     /locus_tag="ACHUZQ_003689"
     CDS             418..873
                     /locus_tag="ACHUZQ_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027290.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(951..1862)
                     /gene="rdgC"
                     /locus_tag="ACHUZQ_003690"
     CDS             complement(951..1862)
                     /gene="rdgC"
                     /locus_tag="ACHUZQ_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1027291.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1987..2895
                     /gene="mak"
                     /locus_tag="ACHUZQ_003691"
     CDS             1987..2895
                     /gene="mak"
                     /locus_tag="ACHUZQ_003691"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1027292.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            complement(3038..4222)
                     /gene="araJ"
                     /locus_tag="ACHUZQ_003692"
     CDS             complement(3038..4222)
                     /gene="araJ"
                     /locus_tag="ACHUZQ_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1027293.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(4348..>7356)
                     /gene="sbcC"
                     /locus_tag="ACHUZQ_003693"
     CDS             complement(4348..>7356)
                     /gene="sbcC"
                     /locus_tag="ACHUZQ_003693"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1027294.1"
                     /translation="LLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKG
                     EAYRAFWSQNRARNQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRF
                     TRSMLLSQGQFAAFLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQA
                     QASGVTLLTPEQVQSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQ
                     QALQQALAEEEKAQPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQ
                     STMALRASIRHHAAKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDR
                     EHLRQWQQQLTHAEQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVP
                     QQKRLAQLMVTIQNVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEA
                     QRAQLQAGQPCPLCGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQL
                     DALTKQLQRDENEAQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQ
                     LRLLSQRHELQGQIAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLAT
                     RQQEAQSWQQRQNELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCL
                     ALHSQQQTLQQQDVLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQL
                     KQNLENQRRQAQTLVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTT
                     SQGEIRQQLKQDADNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGL
                     TLDNLVHLANQQLTRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVS
                     LALALALSDLVSHKTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVE
                     AMKERIPVQIKVKKINGLGYSKLESTFAVK"
BASE COUNT         1699 a         1907 c         1907 g         1843 t
ORIGIN      
        1 tcctttgctg cgattttttc aatttccgat atattcataa ttcatcaagg ttgatataaa
       61 tatcagtgaa gatctccagg tattttttgc ggaactggct acgataaaag ataaatcaga
      121 tgatgaatgg tggcgtgcat tgctgcaaag tcgcaatgat catttgcgcc aaacggcatt
      181 acgcaacgca cataccccgg cgtcgttgtt aacgacattg actgagtctc aggatcggtc
      241 gctggctatt aataatccac agctggctgc cgatgtgaaa acggcgtggt taaaagagga
      301 tctatcatta ctcttatttg tcgaacaacc cgatctttcg cagttacgtg atttagtgaa
      361 aaccggggca acgcggaaaa ttcgcagtga agcgcgtcac cggcttgagg aaaaacaatg
      421 acacaacgtc catggagcaa gttacagcgg gagatctatg acttactgac gccaacgatc
      481 aacctgcaaa ttcactgtac ccgttatctc atgcgcagcc aaaatggtgg aagtaccgat
      541 ttaccgcgtt attggatcac gttggataaa aacgtgatct gggattaccc gaaagacttt
      601 attgcaggta acgctggagt gcgtaatttt cacggtgaaa cttgctggta tccttacctg
      661 acggatattt gttcaatttc tgatttattg cgtgaatata ttgatacccc aaaagcggag
      721 ctgttaacaa aacaatttac cagcgataaa tgggggttag tgaatattct gcgtgctgct
      781 gacaggcgaa tagggatgag gcgattagat cagttacggc gtaaaacgca taatattgct
      841 gcgctaaaaa ttattgctcg ccgtagcgaa taattatatg cctggtgtgg cttcgtacgc
      901 cggataagac gcggcaggcg tcgcatccgg cattaaagga aaatcagcaa ttaacgttgt
      961 gcttcgccac ctaatccttc aatcaggttt tgaattaacg ctgccagttc accagtcatc
     1021 aggatgaaat cggcatcaaa acgcagggcg aaatcttcac ggtcgatatc ttcgttttga
     1081 tcgcgcagct cgtcgcagaa cttaagacgc ttgagcgaac catcgtcgca catcacaaac
     1141 tgaatgcgct gctgccagtc gagcgccagt ttagtcacca cttttccggc ttcaatgtga
     1201 ttggtgatct cttcgctggt cagatcttgt ttcttcgcgc ggatcacgcc gccatcttcc
     1261 agcaacgatt tcagctcggc ttcatcaagc agctggaagc cctgtgccgc actaccggag
     1321 cgaacccatt cggtcagcgt cagttcaatc gggttttcca tgctcaatgg tacaaccggt
     1381 aacgacccca ggcttttacg cagtaatgcc agcgtatctt ccgctttttt ggcactggcg
     1441 cagtccacca taatcaaacc gttaaccgtg tcgatccaca tcattgtctg gctaaaacgg
     1501 ctgaaagcac gcggcagcag agagtgcagc acttcgtctt tcagcgaatc tttttcggtt
     1561 ttcttcagct tacgcgcctg ttccgcttcc agtttggcga ttttcgcttc cagcgcctgt
     1621 ttaatcaccg gagacgggag gattttttct tctttgcgcg cgcagataac aatttgacca
     1681 ttggcaacgt gcgttaacgc atcgctgtgc gatcccatcg gaggaaccca gcccatcttc
     1741 gccatgtcct ggctgccgca tggggtaaat gccatcgagg ctagctgttt ttccatctct
     1801 tctgcacgca gcgaaatctc gcggctaaga cggtaaacca ttaaattttt gaaccacagc
     1861 atgataattt ccacggcctt gtcgttaaat ttagcgggca tgataacgaa ttgtcggcgg
     1921 ccttgcattg ccaatccggt tgtccgtctc tacgctattg atattgaaaa aaataaggag
     1981 agtacagtgc gtataggtat cgatttaggc ggcaccaaaa ctgaagtgat tgcactgggc
     2041 gatgcagggg agcagttgta ccgccatcgt ctgcccacgc cgcgtgatga ttaccggcag
     2101 actattgaaa cgatcgccac gttggttgat atggcggagc aggcgacagg gcagcgcgga
     2161 acggtaggta tgggcattcc aggttcgatt tcgccctaca ccggtgtggt gaagaatgcc
     2221 aattcaacct ggctcaacgg tcagccattc gataaagact taagcgcgag gttgcagcgg
     2281 gaagtgcggc tggcaaatga tgccaactgt ctggcggttt cagaagcagt agatggcgcg
     2341 gcagcgggag cgcagacggt atttgccgtg attatcggca cgggatgcgg cgcgggcgtg
     2401 gcattaaatg ggcgggcgca tatcggcggc aatggcacgg caggcgagtg gggacacaat
     2461 ccgctaccgt ggatggatga agacgagctg cgttatcgcg aggaagtccc ttgttattgc
     2521 ggtaaacaag gttgtattga aacctttatt tcgggcacgg gattcgcgac ggattatcgt
     2581 cgtttgagcg gacatgcgct gaaaggcagt gaaattatcc gcctggttga agaaagcgat
     2641 ccggtagcgg aactggcatt gcgtcgctac gagctgcggc tggcaaaatc gctggcacat
     2701 gtcgtgaata ttctcgatcc ggatgtgatt gtcctggggg gcgggatgag caatgtagac
     2761 cgtttatatc aaacggttgg gcagttgatt aaacaatttg tcttcggcgg cgaatgtgaa
     2821 acgccggtgc gtaaggcgaa gcacggtgat tccagcggcg tacgcggcgc tgcgtggtta
     2881 tggccacaag agtaaaaaac gtaggcaatt ggcgcatcat gcctgatgcg acgcttgccg
     2941 cgtcttatca ggcctacaaa atgtgccaga accgcgtagg gcggataagg cgttcacgcc
     3001 gcatccggca ataagcaacg cgcaccgtga cagtaatcta ccccagtggt ttcgccagca
     3061 ccggactatc cgccgcttgc tggcgcttat agcgaccata cagcagcaac gatgacatcg
     3121 cagcaaacga aagcagggcg gcaggcagcg ccacgtaatt atatgccagc cccagcgtca
     3181 gcatcatacc tccgcaatat gcgccgacgg cgctaccgag gttaaacgct atttgcccac
     3241 ctgcggcacc taataactct ccgcctttgg cgttttgcag taacaatatt tgcagaggtg
     3301 ctgaaagggc aaataatccc gcgcaacaaa taaaagcaaa aataagcgac gttgttttca
     3361 tgccgccgca gaaaaagagc atcagcagtg ccagtacaat gataaagtca gtcactgctg
     3421 caatgcgcag tggtgaataa cgtcctgaaa tcctgccact tagcatattt cccagcacca
     3481 tccctagccc aactaacatc ataataaagg tcatcgccgt ttccgaaaaa acggaaataa
     3541 acatcatgta tggctttacg tagctgaacc aggcaaagac acctgcgttg ccaaacatcg
     3601 tggcggcgaa aattaaccac ggggccgggc tgcgcaaaaa gtgaaattgt tcgcgcagat
     3661 ttcctttcgc ctcgtcgcga atatctggca cccaaaaata gaccgatgcc atcaccgcaa
     3721 tattaaaaac agcgatcaat aaaaaggtgt aacgccagct aaattcctga cttaaatacg
     3781 ttcccagcgg aatgcccagc aaattggcga ctgtcatccc ggaaaccatc cccgccacgg
     3841 cggcggtgac ttttccgggt ttgataattt ttgataacac gatcgcgccg acgccaaaaa
     3901 atgcgccatg cggaaagccg gaaaccaggc gaccaatggc aagcatcagg taagacgaag
     3961 agagcgtgaa catggcgttg ccaatgacgc acaacgccac cagaaacaac aagatatgtt
     4021 tgagtgagta gcggctggaa aagagtgcga tgattggcgc accgaccacc acccccagtg
     4081 cataatacga gatcatatgc ccggcagcag gaatcgaaat tcctacgtta tgcgccagct
     4141 ccgtgagcac gcccataatg ccaaattcgg ccatccccaa accaaacgtg cccagagcca
     4201 aagataaaat gacttttttc ataccaccat cacaaccacc agaacggcta ccaatcaccg
     4261 tagccaataa agtgatagct tgcaggataa cgaccagcaa ggccaagaga taattcgccc
     4321 ctctgtattc attatcctgc tgaatagtta tttcactgca aacgtacttt ccagtttgct
     4381 gtagcccagg ccgttgatct tcttcacttt gatctgcaca ggaatacgct ctttcatcgc
     4441 ttcaacgtgg ctaatcacac cgatggtttt gccactggcg ttcagggcat ccagcgcatc
     4501 aagggcggta tccagcgttt cgctatccag cgtgccaaaa ccttcatcaa ggaacagcga
     4561 gtcaatacgt gttttatggc tgaccagatc cgaaagcgcc agcgccagcg ccagactaac
     4621 gaggaaactt tcgccgccgg aaagggtacg ggtatcgcgt actgcatctg cctgccaggt
     4681 atcaacaacc tcgacttcca gcgcctcgct ggctttgcgc tgtaacagat agcgcccgtg
     4741 cagccgggta agttgctgat tagcgagatg gactaaatta tccagcgtca gcccctgggc
     4801 aaacttgcgg aatttatcgc cctctttgga acctattagc gaattcagat atccccagtc
     4861 ctcaacctgc tgcgtcattt gagcaatttg ctgcagtaag gtttgttgtt gctgacggtt
     4921 atctgcatcc tgcttcagct gctggcgaat ctcgccttga ctcgtggtgt tttcacgcaa
     4981 cttttggtga gtttgcgcta actcttgctg aatctgctcc accgtcacag tgagagccaa
     5041 cccgtcaggt cggtgttgtt gatgctgtgc cagcgtttct gctgtctgtg tgaccagcgt
     5101 ttgcgcctga cggcgctggt tttccagatt ctgcttgagc tgttccagct gcgttagtgt
     5161 ttgttcatcc attagcgccg caaggaacgc ctgctgatcg tcaaagacgc tggcctgtag
     5221 cgcggtgtca aactgcgcct gggctttttg cagactttgc gccgccagaa catcctgttg
     5281 ctgtaacgtc tgctgctggc tgtgtaatgc gagacattgt tcatgaacct gccgccagtt
     5341 atccagcgcc acagtttctt cgctgtgcgg gagatcatca ctttgcggca acgtttccag
     5401 aatcggcgtc agctgctgaa tacggttttg cagcgcggtt aattcgttct ggcgttgctg
     5461 ccagctctgc gcttcttgct gacgtgtcgc caaccagctc tcttcttcat cttcctgtgg
     5521 caatgtcagt gcataacccg tcaatgccgt taagagctgc tgctggcgtt gttcaatttg
     5581 ctgttgatac tggataattt gctgattatg cgcggcaatc tgcccttgta attcatgtcg
     5641 ttggctgagt aaccgcagct ggcgttcgtg ctcatcttgt gcatccagcc acggttgaat
     5701 atcgtcctgt ggctgcaagg tgatattgag gctggtcgtg acggcttgcc attgttgagt
     5761 aagtgcttgc tcatcttgtc ggaggctttg cgcttcgttt tcatcgcgct gaagctgctt
     5821 cgttaatgca tccagttgcc cgcgcagcgc cgcaccttct tcgccgagct ttttaacttc
     5881 gttttccagc gtcagtaatc gagactgatt aacaccaggc tccagcgcct gatacgcctc
     5941 gaccgccggg tggctggtgg aaccacaaag tggacaaggt tgacccgcct gtaactgtgc
     6001 acgttgagct tccagcgttt tgatgcgcgc ttcctgctcg caaatggttt tcacatcggc
     6061 aagttgctgc gtcttttctt tataacgctg gcgcatttcg ttaagtgcga cattacgttg
     6121 cgtctgttcc agagtgacat tctggatagt gaccattaac tgcgccagac gtttttgttg
     6181 gggaacaatc tgcccatgca gcgcgaccag gcgctgacgc agtgggcgtt gctcggaatg
     6241 ttgcgccagg gcgctagcaa cttcatcggc ggttaacgtc aacgtgatcg ccgcaagcgc
     6301 attaagtttt tgctcagcat gggttaactg ttgctgccat tgccgcagat gctcgcgatc
     6361 gctggtttgt tgggagaact gcgcacgcca acccgccagt tcgttgttcc actgacggaa
     6421 gcggtcgtgt tcctgtaacc aggtattcag gctttgttgc tgctgctgta attctgctga
     6481 ctgcttcgcc gcgtggtggc gaatgctcgc gcgaagcgcc attgtgctct gtaagcgagt
     6541 atttacttct tcaatctgct ggcgaatatg cgccagcgcc gcgctgtgtt ctgcgatgcg
     6601 ttcccagtgt ggacgaagat ttcgtgccgg ttgtgccaga ctaagcgccg ccagttgagg
     6661 ttgcgctttt tcttcttcgg ctaacgcctg ttgcaaggcc tgctgacggc ggctggcttc
     6721 ttgctgcaat tcgtcctgac gcgttaacca gtttagcgat tgttgttctt gctgctgcgc
     6781 ggtaattaac tgtttttctt cgtcagtaag tacctgcaaa ctcgctgtca gcgattgcac
     6841 ttgttccggc gtgagcaacg tgacgccgct ggcctgcgct tgcagcttct ccagctctgt
     6901 gcgggccgat ttgtgctgct caaaaaccat cgccgagatt tgcccgtaga tttcagtgcc
     6961 ggttaactcc tcgagcaatt ccgcgcgttc tttgggtttg gcattcagga aggcagcaaa
     7021 ttgcccctgc gaaagcagca tcgaacgagt gaagcgcccg taatccagcc cggttaacgt
     7081 cgctgtcagt tccagcttat ctttcacttt gtcggcgaga attttgccgt cggcgcagcg
     7141 cgccagctct acgcgtggca cctgcaaatt accgtcgggt tggttacgcg cccgattctg
     7201 gctccagaat gcacggtacg cttcaccttt cacttcaaac tccacctccg ccagacattc
     7261 ggcggtatcg cgggtcatga gatcattttg cgattgtgaa acgttagaga gacgcggagt
     7321 ttcgtgatac agcgccagac aaatggcgtc cagcag
//
LOCUS       DAZPBM010000189         7309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPBM010000189 DAZPBM010000000
VERSION     DAZPBM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.190"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            132..1196
                     /gene="ulaG"
                     /locus_tag="ACHUZQ_003694"
     CDS             132..1196
                     /gene="ulaG"
                     /locus_tag="ACHUZQ_003694"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1027295.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1304..2059
                     /gene="ulaR"
                     /locus_tag="ACHUZQ_003695"
     CDS             1304..2059
                     /gene="ulaR"
                     /locus_tag="ACHUZQ_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1027296.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2056..2805)
                     /gene="yjfP"
                     /locus_tag="ACHUZQ_003696"
     CDS             complement(2056..2805)
                     /gene="yjfP"
                     /locus_tag="ACHUZQ_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1027297.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            2987..3316
                     /gene="bsmA"
                     /locus_tag="ACHUZQ_003697"
     CDS             2987..3316
                     /gene="bsmA"
                     /locus_tag="ACHUZQ_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1027298.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3465..3740
                     /gene="yjfN"
                     /locus_tag="ACHUZQ_003698"
     CDS             3465..3740
                     /gene="yjfN"
                     /locus_tag="ACHUZQ_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1027299.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3857..5482)
                     /locus_tag="ACHUZQ_003699"
     CDS             complement(3857..5482)
                     /locus_tag="ACHUZQ_003699"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1027300.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5566..6729)
                     /locus_tag="ACHUZQ_003700"
     CDS             complement(5566..6729)
                     /locus_tag="ACHUZQ_003700"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1027301.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6732..>7309)
                     /locus_tag="ACHUZQ_003701"
     CDS             complement(6732..>7309)
                     /locus_tag="ACHUZQ_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1027302.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1907 a         1952 c         1797 g         1653 t
ORIGIN      
        1 tcaaaatcaa caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc cttcctgaaa
       61 aaatagtgtc aggattaatc aaaaccaatc acatattgat tcgaattgaa ctatcatgaa
      121 ggtaaatggc gatgagtaaa gtgaaaagta tcacccgaga atcctggatc ctgagcactt
      181 tcccggagtg gggtagctgg ctgaatgaag aaattgaaca agagcaggtc gcccctggca
      241 catttgcgat gtggtggcta ggctgcaccg ggatctggtt gaaatcagaa ggtggcacca
      301 acgtttgcgt tgatttctgg tgcggcactg gcaaacaaag tcacggtaac ccgttaatga
      361 aacagggtca ccagatgcag cgcatggctg gcgtgaaaaa actgcagcca aacctacgca
      421 ccaccccgtt tgttcttgat ccgtttgcga ttcgccagat cgacgcggta ctggcgactc
      481 acgatcacaa cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag aactgtgctg
      541 atgacgtacc attcattgga ccgaaaacct gcgttgattt gtggattggc tggggcgtac
      601 cgaaagagcg ttgcatcgtg gtcaaaccgg gcgatgttgt aaaagtgaaa gacattgaaa
      661 ttcatgcgct tgacgctttc gaccgtactg cactgatcac cctgcctgcc gatcaaaaag
      721 cggctggcgt actgccagat ggcatggacg atcgcgcagt aaactacctg ttcaaaaccc
      781 ctggcggttc cctatatcac agtggcgatt cccactactc taactattat gcgaagcacg
      841 gtaacgaaca tcagatcgat gtggcgttag gatcgtatgg tgagaacccg cgcggtatta
      901 ccgacaaaat gaccagcgcc gatatgctgc gtatgggtga agccctgaat gcgaaagtgg
      961 ttatcccgtt ccaccacgac atctggtcaa acttccaggc cgatccgcaa gagatccgcg
     1021 tgctgtggga gatgaaaaaa gatcgtctga agtatggctt caagccgttt atctggcagg
     1081 ttggcggcaa atttacctgg ccgctggata aagacaactt cgagtaccac tatccgcgcg
     1141 gtttcgatga ttgcttcact attgaaccgg atctgccgtt caagtcattc ctgtaatctg
     1201 tcgtttatac cggatagcgt ggagaagttc gcgctatccg gcgtagtatt tcctagctat
     1261 ttcaaatatc atctaaaaaa atcaaatttt atcggaatag ctcatgactg aagcacaaag
     1321 acatcaaatc ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg agaaagtcgt
     1381 tgagcgtctg ggaatttcgc ctgccactgc gcgacgcgat atcaataaac ttgacgaacg
     1441 cggcaaactg aaaaaggtgc gcaatggcgc agaagctatt acccaacagc gcccgcgctg
     1501 gacgccgatg aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta ttgctaaagc
     1561 ggcctcgcag ctggttaatc cgggcgaaag cgtagtcatc aactgcggct ccaccgcgtt
     1621 tctgcttggg cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt atctaccgct
     1681 ggcaaattac ctgatcgatc aagaacatga cagcgtgatc attatgggtg gacagtacaa
     1741 caaaagtcag tccatcactt taagcccgca gggcagcgaa aacagtctct atgccgggca
     1801 ctggatgttt accagcggaa aagggctgac cgcagaaggg ttgtataaaa ccgatatgct
     1861 gacagcaatg gcagagcaga agatgctgag cgtggtaggg aaactggtgg tactggttga
     1921 tagcagtaag attggcgaac gcgcgggaat gctttttagc cgtgccgatc aaatcgatat
     1981 gcttatcacc ggcaaaaatg ctaacccgga aatcctgcaa caactggaag cgcaaggggt
     2041 cagcattctg cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat ctaacgcctc
     2101 aggcgtaatg cggtgacgca cgcctggctg ccatgaacag gttagcagtt tatcccgtcc
     2161 cgtctcgctt aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa cgtcatcgtc
     2221 gaggccatgc cacagcagta gaggtctgtc cccaagttgt tccaggtggt ttgtcgcttc
     2281 ccactctgcc agcggcgcga taatgttatt gaattcattc tgctgtgctg ccgtttcagg
     2341 tatcagcggt ggaaacagtg aacgggcgag cgatgtaaaa tagcccgatc ccatcatgct
     2401 ggcggtacat ttcaccgtgg ggtggcgagc ggtaattccc agtgccgtca tcgcgcccat
     2461 cgacgcgcca ccgactgcca gacggtcatc aagcagccag ttttcttcgg ctattgccgc
     2521 acgtaaagta gtgaattcct gcatactttg tagcaagatt tgccagaatt gatttaaccg
     2581 ccgcgcttcg tcgccactaa aacggctacc gtgatcgggc gcatccggca tgatcacccg
     2641 caaaccagcc tgcgccagcg caacggcaaa atagctatac accaggctgg atgaagtaaa
     2701 gccgtgataa aaaattacgc acggtaacgg ggtatctttt tgcccgacag gataagcatg
     2761 aagaacggga atatctgcca gctcgcgtga ttctatttca atcatctgtc tctccttttt
     2821 gtctggtatg acatgaagaa atccttagca caagtttctg ctggcggtat tagttcataa
     2881 tgttgagatg tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt accaggtaac
     2941 aattcgggaa cattccccca aagttaaatt aataaggcac tacactatgg ttagcaggaa
     3001 acgtaatagc gttatttacc ggtttgccag tttattattg gtgttgatgt taagtgcctg
     3061 tagcgcactg caaggtacgc cacagccagc accgccagta acggaccatc cgcaagagat
     3121 tcgccgcgac cagacgcaag ggttacagcg aataggtagc gtaagcacga tggttcgggg
     3181 ttctccggat gacgcattag cagaaatcag agcaaaagct gtcgctgcaa aagctgatta
     3241 ttacgttgtc gtcatggtgg acgaaaccat cgtgacagga cagtggtatt cacaagccat
     3301 tttgtaccgt aaataaatca gacaacttac atagtcagct ttacattgct ttgcgtccct
     3361 atgcgtttac ctgtgcacaa taaattaccg gctggaagcc tgggcgttta ttaccgcgat
     3421 ggaatgccct gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa caattacttg
     3481 cctcaccctc gctgcaatta gtgacttatc ctgcgagcgc cacggcacag tctgcagaat
     3541 tcgccagtgc tgactgcgtg acgggcctga atgaaatagg tcagatctca gttagcaata
     3601 tttcagggga tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat gaacaaggtg
     3661 cgtcatggta ccgcattatt accatgtacg aagaccagca gcctgataac tggcgcgtac
     3721 aggcgatact gtatgcatga gtgtcatcta cagcaaatga acaatgccgg atgcggcgcg
     3781 agcgccttgt ccggcctaca attttcacat atttcattga gttaatcgaa accagcgtcg
     3841 catcagtcgt atacgtttac aaacacactc cccccgtcgc ccgcagcaat aaatcattct
     3901 ggatctgctc tgacaaccgt acgccgccgc gcgtatctaa catcacctga caccacgcct
     3961 gcgccattgg cggagaagca tatttcaaca tttgcgcacc gcagcccagc aggaatagct
     4021 gatgagtaat ctctcgcccc agttcttcag ctggcttacg cagctgctgc tgtaaacgac
     4081 gaaccgcgcg atcaaaatag cgatcctgcc ctttcacttc cacaaatgct tccgacaata
     4141 agtcgtatac gcccgcttgc ttattgagaa cgcgcaacac atccaggcac ataatattgc
     4201 cggaaccttc ccaaatactg tttaccggca tctcccggta aagccgcggc agctcgctct
     4261 cctcgcaata accaatgccg cccagcacct ccatcgcttc ggccacaaac ggcatacctc
     4321 gtttgcagat cacaaatttt gccgcaggcg taaataaacg cgcccacagg gcttctttgg
     4381 catcggcgcg ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt tgcccttcaa
     4441 gctgaagtgc catgcgactt aagacatgac gcataagggg ctgttggatc aatggattac
     4501 caaaaacatg gcgttgatgt gcatgataaa tcgccagcga aaacgcacgg cgcatcatgg
     4561 catggctacc cagggcgcaa tcaaaacgcg tcatcccccc cattttcagg atcagacgaa
     4621 ttccttcccc ttcctgcccc aataaccagc caatggcatc ctgaaactcc acttcacaac
     4681 tggcgttaga gcgattaccc agcttatctt tcagccgttc gaggcgaatc gcgttgcgtt
     4741 gcccgtcagg caaaaagcgc ggcacaaaaa aacaggacag accgcccgtg gtctgcgcca
     4801 gcaccagatg cgcatcgctt tgcggaaccg agaaaaacca tttatgcccc accagccgat
     4861 aagagccatc ttccaaacgc tctgcacggg tggtgttgct catgacatcg gaaccaccct
     4921 gcttttccgt cattcccatg ccaatcaaca aaccgcgttt ttgcccacct ggcaataagt
     4981 gagaatcgta gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc gtgggtaaca
     5041 tctgtaacaa caatggcgtg gcggcaaagg tcatcgttat tggacataac gacccagcct
     5101 caacctgcgc atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg gagcgagcgt
     5161 cttcttccca ggcaagattg tgcacccgat tggtacataa cacctgcatc agcaggtgcc
     5221 atgcgggatg gaaacgaaca tcgtccagac gccgcccctg ggcatcgtag cgcaacagtt
     5281 caggcgggtt tacattcgcc agccgcccca gttcaaggga ttcagccgtt cctaactgct
     5341 gaccaatact ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt accgcttcgc
     5401 aaagcgcgcc atcagacagg tataagttgc tgttatttaa tggtataggt tgattaaaaa
     5461 cggtgtgagt ttgccagtgc actgtgtctc cctccatcaa tggcagtcac cattagtatg
     5521 gccactgcca tggattcatg acagaaagag attgctaaaa cattcttatc cagcaatgta
     5581 atgcggaacg aaacgtgagg tgtctttggt gatcagtgtg ttatcttcac ggatccccat
     5641 tccgcacgct tcatcatcga caatccagct atcgatgagt gtgtagctat cgccaaatcg
     5701 cggcagaggt tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac catcagcgtg
     5761 gtcaacgacg ttattctgac cgtcgaaaat ggtgacgttt ccgccttcgc gcgagtagat
     5821 cggtttacgc acatagcttt cgccagcggc aatctgcgat ttttcaccct caaaccagga
     5881 tgcaagaagg ttaggatgac cagggaagaa gcgccaaagc agcggcatta gccctttatt
     5941 actcaagata cttttccata acggctccac ccattgctcg cgacgcttgc acagcagcgg
     6001 accgttatcg tcacgcatca tccactccag cggatacagc ttaaatgcac gctggatgac
     6061 attatcatca agatcggtca gtacaccgcc gacgcccaaa ccgagatctt caatgtagat
     6121 aaaccgcgac tcctgccctg cctgctgggc gcagtcctgc aagtacagca cggtagtcct
     6181 gtcttcatcg gtgtcctgac agcagcagaa ataaaacggc tcccggctat aaagctcgct
     6241 aaagcgtgaa atcaggcgtt cctgaatggc attgtactga tcggcatcac gcggaataat
     6301 gccgctgcgc cgggcatcct ccagccacaa ccactggaaa taagccgact catacaacga
     6361 agtcggcgta tcggcgttgt actccagcag cttcaccggc gcattgccac accaggcaaa
     6421 atccatccgg ccatacagcg aaggatcgct ggcgtgccag ctttcggcga tcacatccca
     6481 gtacaacggc gggatcgcca gttgcgtcag gatctcttcg tctttaaccg cgcgatccac
     6541 cacctcaagg cacatctgat gcagttccgc agttggtttt tcgatctgct cttcaatctg
     6601 gcgcagagta aaacggtaag cccgactctc gtcccaatag atttcattgt cgatgatatg
     6661 aaagtcgaaa ccgttgtcag cggcaatttg gtccagatcc cgccgcacag gaacgttgtg
     6721 tctcagcatg attagcctcc ccaatgccca cgggcgctgg aagaacgacc atagccgccg
     6781 cgcgaaacgg ttgacgcttt tttggtggta aagccgcctg aagagtaaga ctcttttttg
     6841 ccagaaccgg agcgccagga gtaatcaccg gaagtgttgc gccagacggg gcgggaagca
     6901 aatgaggatc cgccgctgtt atagacgaac ggctcatcgc gatctttacg aataacccgg
     6961 cttaacaaaa atccagaaac gaccgggatc caactctgtt caacattgtc gtagtagcaa
     7021 ttttcgtact tagactgaca gttctgctga gtcatattct tcggaacatc ggcataaaat
     7081 gccgttttgg cgttgttcca gccacgcgcg caaatgtcgg cattattacc gtcatcaata
     7141 caatcctgca ctgtcgcgta aaacgttccg tcgccgtcgt tatcgacatc gctactatcg
     7201 ccacaacctt tcaatacgaa aaaagcggca ccgcccatta gcgccaacgt taagtatttt
     7261 tgtgcgtagc gattgcgacg cggttcaaaa ggattattcg gtctaccaa
//
LOCUS       DAZPBM010000190         7284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPBM010000190 DAZPBM010000000
VERSION     DAZPBM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.191"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..959)
                     /locus_tag="ACHUZQ_003702"
     CDS             complement(<1..959)
                     /locus_tag="ACHUZQ_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1027303.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1560..1808)
                     /locus_tag="ACHUZQ_003703"
     CDS             complement(1560..1808)
                     /locus_tag="ACHUZQ_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1027304.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2071..2166)
                     /locus_tag="ACHUZQ_003704"
                     /pseudo
     CDS             complement(2071..2166)
                     /locus_tag="ACHUZQ_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2163..2729)
                     /locus_tag="ACHUZQ_003705"
     CDS             complement(2163..2729)
                     /locus_tag="ACHUZQ_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1027305.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3039..4811
                     /gene="aspS"
                     /locus_tag="ACHUZQ_003706"
     CDS             3039..4811
                     /gene="aspS"
                     /locus_tag="ACHUZQ_003706"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1027306.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4929..5381
                     /gene="nudB"
                     /locus_tag="ACHUZQ_003707"
     CDS             4929..5381
                     /gene="nudB"
                     /locus_tag="ACHUZQ_003707"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1027307.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            5410..6150
                     /locus_tag="ACHUZQ_003708"
     CDS             5410..6150
                     /locus_tag="ACHUZQ_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1027308.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHUZQ_003709"
     CDS             6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHUZQ_003709"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1027309.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6708..>7284)
                     /locus_tag="ACHUZQ_003710"
     CDS             complement(6708..>7284)
                     /locus_tag="ACHUZQ_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1027310.1"
                     /translation="EIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGHNGEDFLVAE
                     SRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHTGFKY
                     ESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWERKT
                     VTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1843 a         1636 c         1920 g         1885 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ctgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 cccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcacg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg cgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcggat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gagggggcaa tttttcctta
     1201 aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg tatcagtgaa
     1261 tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt attcttctgg
     1321 aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag
     1381 actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat gattggcggg
     1441 ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg gctggctggt
     1501 gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt acgtgctaat
     1561 tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa
     1621 gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct
     1681 tttacgatca cctctacatc gtcataacgt ttactgatgc gtcgggttaa ttcttccttt
     1741 aacgcatcca cagcaccggt tggcatttta gtcattttct ctttggctat gcagatttca
     1801 atacgcataa aaatccctct acactgtgtt tttatacagt attattttta actgtatgga
     1861 taaacagtgt caagagtgag ggaaagtgat tttttggggc atgcatgggg caaaaatatt
     1921 tgttgtgggg cattttgggg catgattggg acactttgtt acatatgaac tttgccgatt
     1981 ttcatatggc atggcaatcc aacgctttga ttattctaaa gaaaacacat gctcttgggc
     2041 gttctttagt gatttttaaa aatcccgtat cacgcagtta aagtggcggg catactcttc
     2101 aaggctggtg atccccaacc gcacccattt aggatgcgac cattgcggta gaccgatgta
     2161 aatcataacg cgtgaaggat ctcttccacg ctacgcacac gggcgatgcg cgggtagata
     2221 tgattaatgc tgttattgtg ttgctcggcg ctggcggcac tacaggcgtc ttcggcaatc
     2281 accagattaa agccaagttc ccaggcattg cgggcggtgg attcaacacc gatattggtc
     2341 gagatcccac ataacactat tgtatcgata cctcggcggc gcagttgcaa ctccagatcc
     2401 gtaccgtaaa acgcacccca ctgacgtttg atgatttcga tatcactgtc agttgcacct
     2461 aatgcagctg gatgttgcca ccagttttcg ggcaacactt tagcggggga gggggcatca
     2521 accggctgtt ttaatgcttc ggcgtaatcg gcagaccagc caactcgcac cagaaacacg
     2581 ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg cgcgattaac tacctcatcg
     2641 gcagtatgtg gaccaccggc aaaaggtaag atgccttctt gtaaatcgat caccaccaac
     2701 gcagtggttt tagcattaag ttcaagcatg ctgactcctg aaaaaacaaa atcgtgcctc
     2761 acaccttaat gcgattagcc gatggcggaa cgacaaattt tgttaatttt tatgagttcg
     2821 ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc aggcccgata taaagtggta
     2881 acgaggcgtt acagcgcggg ccaggctctt acggtgcggc agaatttcca gtataatagc
     2941 cgcctttttt cattcagttg tgacatacag ctaacgctgc gacttggtca cctgcgcagc
     3001 agggggcatc cgccggcagc taagttaagg gatatctcat gcgtacagaa tattgtggac
     3061 agctccgttt gtcccacgtg gggcagcagg tgactctgtg tggttgggtc aaccgtcgtc
     3121 gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg cgaaggtatc gtgcaggtat
     3181 ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc tgaactgcgt aatgagttct
     3241 gcattcaggt tacgggcacc gtacgtgcgc gtgacgaaaa aaatattaac cgcgatatgg
     3301 cgaccggcga aattgaagtg ctggcgtcct cgctgactat catcaaccgc gcagatgttc
     3361 tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg tctgaaatac cgctacctcg
     3421 acctgcgtcg cccggaaatg gcgcagcgcc tgaaaacccg cgctaaaatc accagcctgg
     3481 tgcgccgttt tatggatgac cacggcttcc tcgacatcga aactccgatg ctgaccaaag
     3541 ccacgccgga aggcgcgcgt gactacttgg tgccttctcg tgtgcacaaa ggtaaattct
     3601 acgcactgcc gcaatcccca cagttgttca aacagctgct gatgatgtcc ggttttgacc
     3661 gttactatca gatcgttaaa tgcttccgtg acgaagacct gcgcgctgat cgtcagcctg
     3721 aatttactca gatcgatgtg gaaacttctt tcatgaccgc gccgcaagtg cgtgaagtga
     3781 tggaagcgct ggtgcgtcat ttgtggctgg aagtgaaggg cgtggatctg ggcgatttcc
     3841 cggtaatgac ctttgcggaa gcagaacgcc gttatggttc tgataaaccg gatctgcgta
     3901 acccgatgga actgaccgac gttgccgatc tgctgaaatc tgttgagttt gcagtatttg
     3961 cggggccggc gaacgatccg aaaggccgtg ttgccgctct acgtgttccg ggtggcgcat
     4021 cgctgacccg taagcagatc gacgaatacg gtaacttcgt taaaatctac ggcgcgaaag
     4081 gtctggctta catcaaagtt aacgaacgcg cgaaaggtct ggaaggtatc aacagcccgg
     4141 tagcgaagtt ccttaatgca gaaatcatcg aagccatcct ggatcgtact gccgcgcaag
     4201 atggcgatat gattttcttc ggtgccgaca acaagaaaat tgttgccgac gcgatgggcg
     4261 cactgcgcct gaaagtgggt aaagaccttg gtctgaccga cgaaagcaaa tgggcaccgc
     4321 tgtgggttat cgacttcccg atgtttgaag acgacggtga aggcggcctg acggcaatgc
     4381 accatccgtt cacctcaccg aaagacatga ctgctgcaga actgaaagct gcaccggaaa
     4441 atgcggtggc gaacgcttac gatatggtca tcaatggtta cgaagtgggc ggtggttcag
     4501 tacgtatcca taatggtgat atgcagcaga cggtgtttgg tattctgggt atcaacgaag
     4561 aggaacagcg cgagaaattc ggcttcctgc tcgacgccct gaaatacggt actccgccgc
     4621 acgcaggtct ggcattcggt cttgaccgtc tgaccatgct gctgactggc accgacaata
     4681 tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc gtgtctgatg actgaagcac
     4741 cgagctttgc taacccgact gtactggctg agctgagcat tcaggttgtg aagaaggctg
     4801 agaataactg atatgactca aatacacgaa atcattcgcg ttgcatcaag gcggcaactg
     4861 agtgaactcc catgagcata gataactatg tgaatgggat gagcgaaggc agtcaacgaa
     4921 gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc gatcttagtg gtcatctatg
     4981 cacaagatac gaaacgggtg ctgatgttgc agcggcgtga cgatcccgat ttctggcagt
     5041 cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca agctgccatg cgcgaagtaa
     5101 aggaagaggt caccattgat gttgtcgctg aacaactgac cttaattgac tgtcagcgca
     5161 cggtagagtt tgaaattttt tcacatttac gtcatcgcta tgcgccgggc gtgacgcgta
     5221 atacggaatc atggttctgt cttgcgctcc cgcacgaacg ccaggtagtc ttcactgaac
     5281 atctggctta caagtggctt gatgcgcctg ctgccgcggc gctcactaag tcctggagca
     5341 accggcaggc gattgaacag tttgtaatta acgctgcctg aacaggcagg gctatctgga
     5401 gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa agctgcgcag
     5461 gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac cgcggctaag
     5521 ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg cggcggtgga taaagcactg
     5581 tctaacaaca tgacccgtga cacactgaac cgcgcaattg cacgtggtgt gggcggtgat
     5641 gatgatgcaa acatggaaac catcatctac gaaggttacg gtcctggcgg cacggcaatc
     5701 atgattgaat gtctgtctga caaccgcaac cgtaccgttg ctgaagtgcg tcatgcattt
     5761 agcaaatgtg gcggtaacct cggtactgat ggttccgttg cctatctgtt cagcaaaaaa
     5821 ggcgtgatct ccttcgagaa aggcgatgaa gacaccatta tggaagcagc actggaagca
     5881 ggtgctgaag acgttgtgac ctatgatgac ggcgcgattg acgtctacac tgcatgggaa
     5941 gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc tgaaagcaga cagcgcggaa
     6001 gtttccatga tcccgtccac caaagctgat atggatgcag aaaccgcacc gaaactgatg
     6061 cgtctgatcg atatgctgga agattgcgac gacgtgcagg aagtctacca taacggtgaa
     6121 atctctgatg aggttgcagc aactctctga tggggcctgc taaacagcaa aacggagacg
     6181 cgtgatggct attattctcg gcattgaccc gggttcgcgc gtgatcggct atggtgtcat
     6241 ccgccaggta ggcaggcaac tgtcctacct gggtagcgga tgcatacgca ccaaagtgga
     6301 tgatttaccg tctcgtctga agctcatcta tgcgggcgtg acggaaatca tcacccagtt
     6361 ccagccagat tatttcgcca ttgaacaagt ctttatggcc aaaaatgccg actcggctct
     6421 gaaactgggg caggcgcgcg gcgtggcgat tgtggcggcg gtgaatcagg aattgccggt
     6481 atttgaatac gcggcacgtc aggtaaagca aacggtggta ggtattggta gtgccgaaaa
     6541 aagccaggtg cagcatatgg tccgcacctt gctgaaactg cccgctaatc cacaggcgga
     6601 tgccgccgat gcgttagcta tcgcgattac ccattgtcac gtcagccaga atgcaatgca
     6661 gatgagcgaa tcgcggctga acctggcgag agggcgactg cgttaagtta taccgcctcg
     6721 gtcagttcag gctgaggcgt ttcaactccc tccgcgtgaa tcaacaccag acccggatga
     6781 tgccacaaac tggctggcgt gacggtttta cgctcccacg gaatagaacc taagaaccag
     6841 aatttccaga aagtgagttt tgcattcgga ttgctattta acagttctcc aaacgtttct
     6901 atttcaccca ccggaatgca tagcgcttct ttataaatat caaaaacaaa ttttgaacag
     6961 aactggcgcg aagattcgta tttaaaaccg gtgtggtaaa gtttgcgtag ccgggaggga
     7021 acctgttcaa caattcgttg tttttgttgt tctgtcagcc cagcgtctaa tcgctttata
     7081 gcatagcgtt gattagacga acgtttaata aaacgagata gcgtggtgat ggttgaaagg
     7141 ggaacccggc tttctgcaac caggaagtct tcaccgttat gaccgataat gatcccgacg
     7201 tgattactcc agcaatttga tgcagctgat atttgaccaa ataaggcagc acctatacat
     7261 gtaaagacga tatctccaat ttca
//
LOCUS       DAZPBM010000191         7272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPBM010000191 DAZPBM010000000
VERSION     DAZPBM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.192"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(1..4518)
                     /locus_tag="ACHUZQ_003711"
                     /pseudo
     CDS             complement(1..4518)
                     /locus_tag="ACHUZQ_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4538..4999)
                     /locus_tag="ACHUZQ_003712"
     CDS             complement(4538..4999)
                     /locus_tag="ACHUZQ_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR1027311.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6929)
                     /locus_tag="ACHUZQ_003713"
                     /pseudo
     CDS             complement(5027..6929)
                     /locus_tag="ACHUZQ_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1380 a         2209 c         1961 g         1722 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgacgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cctggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg ttattcagga
     7141 caggtacgca tatcacaacg agtaacaaaa acgcaaagtt aaaccaatat tcataatata
     7201 tttttattca cagcaataaa taatccaatt aacattatta aaatgaatga ttgcataaga
     7261 aatatatgct ac
//
LOCUS       DAZPBM010000192         7121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPBM010000192 DAZPBM010000000
VERSION     DAZPBM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.193"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHUZQ_003714"
     CDS             <1..435
                     /locus_tag="ACHUZQ_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1027312.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="ACHUZQ_003715"
     CDS             518..1420
                     /locus_tag="ACHUZQ_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR1027313.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHUZQ_003716"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHUZQ_003716"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1027314.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHUZQ_003717"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHUZQ_003717"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1027315.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHUZQ_003718"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHUZQ_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1027316.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="ACHUZQ_003719"
     CDS             complement(5330..6037)
                     /locus_tag="ACHUZQ_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR1027317.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHUZQ_003720"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHUZQ_003720"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1027318.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="ACHUZQ_003721"
     CDS             complement(6895..7098)
                     /locus_tag="ACHUZQ_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1027319.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1990 c         1855 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggacgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAZPBM010000193         7026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPBM010000193 DAZPBM010000000
VERSION     DAZPBM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.194"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(9..2792)
                     /locus_tag="ACHUZQ_003722"
     CDS             complement(9..2792)
                     /locus_tag="ACHUZQ_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1027320.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2849..5221)
                     /locus_tag="ACHUZQ_003723"
     CDS             complement(2849..5221)
                     /locus_tag="ACHUZQ_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1027321.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5259..6944)
                     /locus_tag="ACHUZQ_003724"
     CDS             complement(5259..6944)
                     /locus_tag="ACHUZQ_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR1027322.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1822 a         1597 c         1575 g         2032 t
ORIGIN      
        1 ggctgttatt attttggcaa taatactcca gtataagtat ttaccggatg agaaagatat
       61 tgtttaacgg cagtgttaac attctctacc gtcatttgtt tcaacaattg ctcctgctca
      121 gtccatgctg caggatcgtc atattgaata agactattta caatagtgtt cgctaattgt
      181 tgaacgctac gctgttggat atcgaggctg cgctgaacgt tttgctggta ttcattcagt
      241 tcttgctcac tgatcccttt agccagacgc ttaaccatca cttcattcgc taacgttaac
      301 agttcatcat gtcgttctgg ttgacaagta aaagccagca aatgactgat atctttggcc
      361 tgaggatcaa ccgagaggcg agaagaaacg ctgtatgctc cagatgcctg ttcacgaata
      421 ttaacgcgta gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat acgcgtcgcc
      481 agattaacag gtgtccggga atcataacgc ttccactgtg aaacctgtgc cacaggttca
      541 ttttgttctt ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg tttacctgcg
      601 gctaatggcg aatcagagtg tttgattgat cctaagtaac gcgtaattaa cgccacgagt
      661 ttgtcttctg agacattacc gacaatgaca aacgtgatat ccgctggaga tgaaaacaac
      721 tggcgatcgg cagccagcgc atctgcggca gtaaactgta caatctgatt ttcttgcagt
      781 aatttcgtgc ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa tttctccgct
      841 ggacgctggt cgagcgtttt caacgcctga atttgagcat tttgtagcga tgcccaaata
      901 ttgtcgttaa tcgtgctgtg ggtgattcgc tggttaatca actgaaaacc aggttcaggg
      961 ttattagtcc gcgcgctaac ggagagcaac gtattcatgc cactgacttt actgctcatg
     1021 gttaccgaat tttccgcact ccagcgtttc aggctggaag aggagagctc accaacgccg
     1081 cttccgctta ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc agggaaactt
     1141 aaatcgcctt tattagatac ggcagtaatt tgtagctttt gctcttcacc cgcggatttt
     1201 gccagaataa ccctggcacc attggaaagt gttaatgatg tcagattctc agccagcgtt
     1261 tctttgctgc taatttccgc ctgtgggtca gcgtctactg ttaacgataa atttctgcct
     1321 gggaagatgt aagccgccag ctttttgttg gcgtactctt tttccagcgc caggatagcc
     1381 gcaagagaca atgctttttt ggcggcaagc tcattgttta ccatttgctc ccaaaatgcg
     1441 tcctggttct ttcttaactg ctgccatttt tccgccagac tttgcacggt aatttgctgc
     1501 cacagacgtt tcgaaagttg atatgtctct tccggcgaca agaacggcgt attatttaat
     1561 gagctggatg ccaggcgact ggtcagcata cgtaaatcac gttcagcttg ctgatcaacc
     1621 gcatttttca gccaggtgag gcgggtagat ttgacatcat cgagttcttc agcagaaaaa
     1681 ccatgctgat caatggttgc caactctgcc attaatgcat ttgcagcatc ctgcatatta
     1741 tcgtctcgtg catttacacg gaaaaacagc gactgataat cgggtgcaat tttaacgctg
     1801 cgcgcagtgc cgccagaaat agtcttcaac tcgcccgact gtatgcgttc ctgcagacgt
     1861 tgattgaaca gctgaactaa catgctccat tcagcttgtt cgacaaagct ttgctcatcg
     1921 ttcacttgta ccattggcag gcgataatag agtgcgatgc cgttcactcg gttttcttta
     1981 tcattaatga tattaaagcg caggtggttt tcggcttttg tcggccagac gcgattttca
     2041 gctgctttgt tagccggaag cttacttaaa ttatccttta tcagcgccag cgcttcttta
     2101 ctgtcgatat cgccgaccac gataaaggtc atattatttg gttgatacca gcgttgataa
     2161 aattggcgca attgtgccgg tgtgaccgtg gcgacagtat ccatcaggcc gataggttca
     2221 cggtctaaat tacgggtatt tgccagcagg aaagggcggc gcgcctgaga ggtgcgccat
     2281 ttcgcatcct gatgggcacg ccattcctca gtaattacgc cgcgttcagc gtctacttcg
     2341 agtatttcaa aggttgcggc attactccat tcactgaaga ttgccatcac ttgttgcaga
     2401 ttttgtttct gcgtagtcgg cagactcacc tgatacagcg tttcgtcata gctggtatag
     2461 gcattaacat cgcgaccgaa acgcaggccc attgactcaa atgtttcgat gactttatta
     2521 cccggccatg tttttgtgcc gttaaacatc atatgttcta caaaatgagc cacgccgcgc
     2581 tcattgtctt cttcctgcaa tgaaccggta tgaatttgca gccataaatt tacctgatcc
     2641 tttggttgag catgcggata aatcatatat cgcaagccat tgtccagttg cccggtaatt
     2701 aacttttcat cctgcggtaa ggcggcggca atcagccgcc cggggagcaa cagagttgcc
     2761 actaacgtca gtaagaaaca gaggtttctc ataattatct ccatgcgaaa accgggcgaa
     2821 tttacccggt taagtaaaat ccgaactatt aaaatttcat gctgacatca agccagaaag
     2881 tacggccact ggcgtaggtc gccacaccgg tgttcgttgt atcaacagcg gttttgctat
     2941 ccagtacatt gagaatatcg gcactgatcg tcaggttttg ttgtttcaga aattgtggcg
     3001 tccaggacaa gcgggtgtcc caggtcaggc tgctatccag tttttcgtca acatactgct
     3061 tgtaatctga atattcgctg atgtattgcg catttgtctt accaaggata atgcgagctt
     3121 tacgcgcttc ttgccaggcc agcgtattcg cccacactaa cccgctcggt tgatgcgtga
     3181 aatccatgtt taaggagatc tttaatgggt tattaaaatc tgccactgga acactatcgt
     3241 aagagaccag attaccgtta taaaccacct ggttatcacc ggtattgctc tcctcataac
     3301 cattgtttaa cgacaagttg cccttgctct tgatatagct aaagacaatt tgtgggttaa
     3361 tatctacttg gcggatatgc aggggttcgg ccagttcaaa actgaggctg aacgaatgcg
     3421 ttttggtttt gccatcgttg ttatattcag taatggtggt tttagtcgcg ctgtcggtac
     3481 gactgctttt gctgatttga tcatgcgctt cacggtaaac atagtttgca cgcgcaataa
     3541 cgttcttacc gattttctgc tgcaatccca ttgccagttc atcgttataa ggcgttttca
     3601 aatcctgata acgcgtcaga gttttattac ctgataccga ttccgtccag ctattgcgga
     3661 tatcacgtaa tcccatatca agaatattcc cgccatagta acggttataa ccggcggtaa
     3721 tcattgaggt ttgatcagca aaaatatccc attccgtcat aaagcgcggg gagatattgt
     3781 ggtttgacag atagttgtca tagtcataac gcacaccggg cattaacgac acattccgcc
     3841 agctaatgtg ctccgccata tacagcgtgt agttgtcaat tcccaggctg cctttacctt
     3901 tatggtaaat ggtatggtta gttttctttc ctgcagcatt aatcacatag gattcagact
     3961 ggttatggcg ttcagtccat gcatcggaat agatgtattc cgcgccgaag tagggttgat
     4021 gcgaaacatc acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat
     4081 ctacagcctg ggaaatgtgt cctaatccgc cacaggtaca acgccctgta atatcaccat
     4141 atgtacatga aagttcggtg taccagatgt catgatcgtg acgggtataa tcactaatat
     4201 gatcccaacc aacggtggta cgtagtttgg cccatgcgag ttgcgtatcc atatcccatg
     4261 ccagaccata ggatttatta cccatttcgc gatcagactg cgggaaggtg ctggtattat
     4321 aatcacggct ggagccggta tattttaagg ttaaatcgtg ggtaaagcgg tcgctggcaa
     4381 accaggtaaa tttgctcaat gcagtatcga taacgttttt atactgtgcc cgaccggtga
     4441 caatgccgtc attcgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac
     4501 cggcggtaac gccgaagtta tcagcgagtt cctgattaaa cgacaaggta taaaagttct
     4561 ttttaaaatc tggggagtaa taagtacttc ctgaagagcc ttgattaaat gcgctcttgt
     4621 tattctcatc gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa cccaatttca
     4681 ccttgctatc atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc
     4741 gaccaaattc aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat
     4801 caagataata cccctgtgac atcccgctaa tattggttgc actactggca tcggactcat
     4861 tcgctgggtt caggttatta gttgcactaa taccgtcaat caaataggca ttctggtagg
     4921 gcgacgcacc gtgaatagag attttctcag ggcgaatatc tccctggttc aacgaggtac
     4981 tttgcgttga atccatgcgt accgcagggt tggttctcag cagatcgctg atattgccat
     5041 tcccggtcgg taaacgctcg atgctttcac tggtgtaatg cgtgttgccg tttacgggta
     5101 cagggacagg agcatagacc gtcatctctt caaaaaaatg catgtacatg tttttgtcat
     5161 cgacggcctg cgccacatca gcgccacata aaatgacgcc aggaataaga actcgcttca
     5221 tttatttata tctccgtaca gcagggcttt tattgttgtt ataaaaccgt gctaatgtca
     5281 caaatatcat cagccaggtt ccagacaccg ggttgatgtg taaccataat gacgccgctt
     5341 gtgggtagtt tttcacgcac taaacgcagt aagcgtatag cctcttgttc ctcaagatga
     5401 gaggtagttt cgtcaagaaa tatccatttc ggacgtcgta aaattaatcg tgccagggcg
     5461 atacgttgtt tttcgccgct ggaaagaata tctccccagc gatcgtggtc atgaatacgc
     5521 gcagccaatt tccccagacc aacctgatgc agtacttcgc tcaacgattt atcgtctacg
     5581 ggcaggggaa gtgctttaca aataatctct ttcagtaagc cgcttttgat taacggtgtt
     5641 tgtgacacat accaactgtc agcaggagaa gaaatatcac ctttaaacca cggccagcag
     5701 tgggataatg ttttaagcag tgtggttttt cccgcaccag agtagccttt cagtaatagc
     5761 catttgcctg gcgaaacatg aaagttcaag ttctctaata tgatcttatt atcaggcgta
     5821 cgaatactcg cattagccac ttgtaccgca tgttggcaat ttttaggctt attcgtaggg
     5881 cgctgttcag tgagttgatg gaactcatac aagcgatcga taaccgcagc cagttcagca
     5941 agttcgtcat atttataaat aaaccagctt aaattgttcg atactagcat aaatgcctgg
     6001 cgcgatttca tcagtccgcc cagattgatc tgcccgctaa taaactgcgg caataacaga
     6061 aagtagggaa gaacgctaag cgagcgcgaa taaatatttt gccagtaatc aagccaccgc
     6121 tgacgattca ttaaacgatg ccagttctct ttaatggtat gaaaattgtc gcgcaactcc
     6181 tggcgttgta gagactcagc attgcttagc gcaatcagtt ctgtctgctt attatgctgc
     6241 acaagattag ttcgaaatgt cgcttcgcta cgttgttttt ccacattaag cggacgaata
     6301 cgtttaccca ccttatgggt aaataaagtt ccaccgatca caatgagcac gacggtatag
     6361 accatatacc cctggatatt ccattctgtt ccaccaacag tgaatgagag cgtacccgcg
     6421 ctttgccaaa gaataacggt aaaggtgatc agcatgctaa gtgactggat gaagccaaat
     6481 gaaagactga gcgttttgct gatcagtaag agaatgtctt cagcgatacg ttggtcaggg
     6541 ttatctgtat ttttatgctc gccgtagatc tgcgtgaagt agtagttttt atctgcaaac
     6601 cagcgattaa ggtagtaatc tgttaaccat tcgcgccagc gaatagtcag tagtttaatt
     6661 aaccatgttt tgtttacgga tattaataca aagatcccca gcagggcagg gaaccaaaga
     6721 acaagctgcc aaagcttatc ggtttctttc tggcttagcg cattgaaaaa atcattattc
     6781 cagtcgttta accagacctg aattttaacc acgccaagga tcattgcgag gataataatg
     6841 attaacaaca ctgaagtttt attattctta cgcagccaaa agggcttcag cagacagaga
     6901 tactttgcta tcaacatata aaacgtaatg ggaatggtta tcattagcga aaattgattg
     6961 tgccatccaa tgattatcta tggcaatgat ttactgatga aatttattta agtatcattt
     7021 aacaat
//
LOCUS       DAZPBM010000194         6825 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPBM010000194 DAZPBM010000000
VERSION     DAZPBM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.195"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHUZQ_003725"
     CDS             complement(155..667)
                     /locus_tag="ACHUZQ_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1027323.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHUZQ_003726"
     CDS             862..1626
                     /locus_tag="ACHUZQ_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027324.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHUZQ_003727"
     CDS             1577..2980
                     /locus_tag="ACHUZQ_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1027325.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHUZQ_003728"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHUZQ_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHUZQ_003729"
     CDS             4337..5080
                     /locus_tag="ACHUZQ_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1027326.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHUZQ_003730"
     CDS             5084..6304
                     /locus_tag="ACHUZQ_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1027327.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHUZQ_003731"
     CDS             6308..6814
                     /locus_tag="ACHUZQ_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027328.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1815 a         1622 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacga
//
LOCUS       DAZPBM010000195         6823 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPBM010000195 DAZPBM010000000
VERSION     DAZPBM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.196"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHUZQ_003732"
     CDS             complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHUZQ_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1027329.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHUZQ_003733"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHUZQ_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR1027330.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHUZQ_003734"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHUZQ_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR1027331.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHUZQ_003735"
                     /gene_synonym="cse2"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHUZQ_003735"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR1027332.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHUZQ_003736"
                     /gene_synonym="cse1"
     CDS             complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHUZQ_003736"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR1027333.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4156..>6823)
                     /gene="cas3"
                     /locus_tag="ACHUZQ_003737"
     CDS             complement(4156..>6823)
                     /gene="cas3"
                     /locus_tag="ACHUZQ_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR1027334.1"
                     /translation="KNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKR
                     NCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKN
                     YEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMD
                     ELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADW
                     TGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIENPT
                     PLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMAT
                     ANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFS
                     QGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACD
                     AYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDY
                     PWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSV
                     DDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDSSQRAGKVLI
                     CTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELL
                     ILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESV
                     YGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAE
                     ESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIE
                     QRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1649 a         1857 c         1620 g         1697 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt tttattgagc agcagcggga aatgatgttc gtaccaacag
     2941 cgcgctttca tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg
     3001 agactgctgg cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac
     3061 ttacccgttt cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg
     3121 accgagtaaa actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc
     3181 cacatggcat agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc
     3241 tgctcaccgc aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg
     3301 cgaggcattc cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg
     3361 gccagttcgc tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg
     3421 ggtagcggta aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag
     3481 ggagtttgtt gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg
     3541 ccgcgtaaac cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac
     3601 agagctaacg cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg
     3661 tctttattaa acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga
     3721 actttatctc cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac
     3781 tggaaagcat gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct
     3841 tcccagatat catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc
     3901 agcaaaaact gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca
     3961 ttttcatccg ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg
     4021 ggcaaccacg gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga
     4081 ccatcccagg tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt
     4141 cctgtcgcag acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccgca
     4201 actttcgtca tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat
     4261 gcaccacagc ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca
     4321 ccagcttcgg cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg
     4381 cttcaccaca ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg
     4441 tgtcgacagt ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt
     4501 cagcacaaac tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc
     4561 gctgcgggca aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag
     4621 ccgggctgcg tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca
     4681 gattcgccca tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc
     4741 gtcgccagga gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc
     4801 gtcctttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc
     4861 tgctcgctga atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca
     4921 atcgacactc tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct
     4981 atcttcttta ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc
     5041 aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg
     5101 acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg
     5161 acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct
     5221 gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt
     5281 gacatgcgtc agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc
     5341 tgtaccccgc gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga
     5401 aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca
     5461 cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc
     5521 cagaagccgc agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt
     5581 gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca
     5641 tccctgactg aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag
     5701 tgattcatta aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg
     5761 ggattctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc
     5821 cgttgccatt gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct
     5881 gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa
     5941 taactgcggt ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg
     6001 attctcaata aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag
     6061 aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat
     6121 atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag
     6181 cacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc
     6241 atcccagaat gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc
     6301 tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg
     6361 ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg
     6421 cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa
     6481 ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat
     6541 cttgagttca gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg
     6601 ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg
     6661 acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc
     6721 tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg
     6781 tcgggttttt ccccagaaat caaaaaaagt cacaatattt tta
//
LOCUS       DAZPBM010000196         6808 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPBM010000196 DAZPBM010000000
VERSION     DAZPBM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.197"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(257..742)
                     /gene="sixA"
                     /locus_tag="ACHUZQ_003738"
     CDS             complement(257..742)
                     /gene="sixA"
                     /locus_tag="ACHUZQ_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1027335.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(945..3089)
                     /gene="fadJ"
                     /locus_tag="ACHUZQ_003739"
     CDS             complement(945..3089)
                     /gene="fadJ"
                     /locus_tag="ACHUZQ_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1027336.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3089..4399)
                     /gene="fadI"
                     /locus_tag="ACHUZQ_003740"
     CDS             complement(3089..4399)
                     /gene="fadI"
                     /locus_tag="ACHUZQ_003740"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1027337.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4579..4863)
                     /locus_tag="ACHUZQ_003741"
     CDS             complement(4579..4863)
                     /locus_tag="ACHUZQ_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1027338.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5235..6575
                     /gene="fadL"
                     /locus_tag="ACHUZQ_003742"
     CDS             5235..6575
                     /gene="fadL"
                     /locus_tag="ACHUZQ_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1027339.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1694 a         1821 c         1622 g         1671 t
ORIGIN      
        1 tgattattca actctatttt ctctattatc tgtattaata aatgacaatg agtgtcaaac
       61 agacattatt catttcaaca tgtaactttg ggcaaaatca agatacgcgc agaagaataa
      121 tttccgaatg aaagtagaaa gtgttttatt tatagatggg gagagtcttc gatagtagtg
      181 agattgacag atatccgctg cctgatgagc aattatgccc aatgaatcta cctcattggg
      241 cataatttgg aactcatcag atagcttttg ccatcttcag attacacggg ctcatctgcc
      301 agttgaatgt gccgtttccg ctttcatcaa gcgttacgct ggcaatggcc gaggtggtaa
      361 acataggcgg cgtttcgccc ggacataact cggcaaccag atacccgact aacggtaagt
      421 gagagatcac cagtaccgaa gcaacacctt cattggtcaa cgcctgcaaa taggcactga
      481 ccagaccaac atcgccgcag ggcgttaact ccggcagaac ttctgcactg gaaggcaggt
      541 tcagacaatc ccctacttct tccagtgttt gctcggctcg caggaacggg ctcaccagaa
      601 cgcgttcgat ttccactttt tgacctttca gccagttcgc catcaggcga gattcgtcac
      661 aaccattagt ggtcagagga cgaaccgaat cactggcggc atcaagggct gcgtcgccgt
      721 gacgcatgat aaaaacttgc atattgcacc gcttttgtta accagattcg tccgttttct
      781 ttcatctgaa accagagaaa gaaaacggtg gccggcattg tgccttatcc attcaccgaa
      841 tgaaacgctg ttttttacct caatggcgta agtatagtca atatgcgttt acattttgac
      901 caacactccg ccattcagcg cggattcata tagctttgac cttcttattg aaggtcagtt
      961 gcagttgttt tccaaaaact ttccccacgc gcgcccatct cgaccaaacg ctcgcaaggg
     1021 gtaaaacggg aaccatactg cgtggccagt cgttgcatta ttgcaaccac ttcgcccgcg
     1081 ccgagagaat cgatatagcg gaacggtcca ccgagaaatg gcggaaaacc aatgccaaat
     1141 accgcgccaa tatccccgtc acgcacgcta cggataacct gctcatcaac acaacgtact
     1201 gcttcattca gcatcaacat cacacaccgt tcagcaacct gcggtgcgga gagtcgcccc
     1261 tgcccttgtg cgccaatgag cgggtaaatg gcgggatcga cctgtttttt gcttttacgc
     1321 cctttctgac cataaagata gaaaccccga ccattttttc tgcctttgcg atcgtcgttc
     1381 aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt ctccataagc ggcttccagt
     1441 acaggaataa ttttagtccc ggtgtcgatt cctacctcat ccaaaagttg gattgggcct
     1501 accggaaaac caaatttcac tagcgcggca tcaatgtgct ctacccgttc accttcggtc
     1561 agcatgcgga tagcttcatt aatgtaaggc gctaagatgc gattgacgta aaacccggct
     1621 ttgtcacgca cgacaatggg cgttttgccc tgttttttcg ccagttttac cgtagtggcg
     1681 atggtttgcg ccgatgtacc cgcatgagga ataatctcta ccagcggcat tttttccaca
     1741 ggactgaaga aatgcaggcc aataacttgc tcaggtcgcg cggcgtgagc ggcgatatca
     1801 ccaatcggta aagatgacgt gttcgaagca aaaatggtat gagcggcgca attttgctcc
     1861 acttccgcca ccatctgttg ttttaattcg agattttcaa acaccgcttc aataatcaga
     1921 tcgcgatggg caaagccgcg atagtccgtc gttccggaga ttaatgccag ctgtttgtca
     1981 cgttcgctgg ctttgagatg acgacggcga actttgccct ccagctgatc ccaactgtac
     2041 ttcagcgcat gatttatgcc ctgcgggttg atatctttaa ttctgaccgg aagccccgct
     2101 ttacaagcag tgacataagc aataccgccg cccatcaagc caccacctaa aattcccacg
     2161 ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt tcttcacgtc cgtactggca
     2221 aaaaagatac tacgcaaagc ttgcgattgc ggcgtcatcg ccagttcgcc aaaggcgcga
     2281 gcttcagcgt catagccgct gctggtgccc tgcgctaatc ccgtttcaac aacctccagg
     2341 atgcgttctg tcgccggata attgccttga gttttgtgtt ctgttttctt gccgaccatt
     2401 ttgaacagca gcgcacgacc taacggcccc gccagaatac gctcgcgtac aggtagaggg
     2461 cgggaagatg ggcgatcctt cttcgccagc tcaacagcgg cttccagcaa aatggagtgc
     2521 ggaacaacgt catccaccag ccccagcttt aatgcctgtt tcgcccgaag ttgttttccg
     2581 gtgagaatca tctctaatgc tgtactgacg cctatcagac gcggtaaacg ctgggtgccg
     2641 cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac cgagcaccgt tttaggatcg
     2701 tcagtacaaa cgcgaccgtg gcacgccagc gccagctcca gcccaccgcc caggcaagcg
     2761 ccatgaatag ccgcgataac cggaatgggc aaagcatgaa tctccgccat caactgttgg
     2821 ccctgccgcg ccagtgcttc cgcttcttgc gccgttttgc agttgccaat catgttgatg
     2881 tctgctccgg cgataaaatt gtccggtttc gcggagataa acaccacgcc gcgcaactct
     2941 ttgttttcac ggagttgctt aataatggcg cgcacctgcg aggcaaattc cgccttcagg
     3001 gtattcattt tctcatccgg tacatcgatg gtgatgatag caatgttgtc cagacgaaca
     3061 ttaagagtaa acgctgatgc catttccatt attccgcctc cagaaccatt gccgcgccaa
     3121 gcccaccggc agcacaggcg gtaactaaac caaatccacc gccgcgacgg cgaagttcat
     3181 gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa gggatgcccg taagcaatcg
     3241 aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc agtggcatgt gcacgcccca
     3301 gtacttcacg agcaaaacgt tcactaccca gcaactgaat attcgccagt gtctgagctg
     3361 caaaggcttc gtgcatatcg atcaatgtca gttcgctcat cgtcaaaccg gcacgttcca
     3421 gcgccagcgg tgttgaccag gctggaccga gcaacatgtc ctgccagaca tcaatcgcag
     3481 taaatgcgta gctgcgcaga taccccagcg gcaccagccc taattctttc gcccgggatt
     3541 cagtcatcag gatcaccgct gccgcgccat cggtcagcgg cgtactgttt gccgccgtta
     3601 ccgttccgtg tttgcgatca aacgccgggc gcagctttgc gtaatcggca agcgaggaat
     3661 taccgcgaat attgttgtct tcgacaagcg gttgtttata aggagggata aaggcagtca
     3721 tcacctcttc tttgagtttt ccgtctgacc atgcctgagc ggcacgctga tgcgaacggt
     3781 gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt tttcgccatt tgttcggcgg
     3841 tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc aggcggtacg ggcattaagt
     3901 cgcgcaaacg caggcgagag aagagtttca ggcgctggct catggttcga gctttgttga
     3961 catcaaccag cacgcgcgcc agttttttac tgacgccaat tggcaatacg gaagaggaat
     4021 ctgccccacc ggcaatcccc gctcggatag ttcccgccat caggctttct gcgacgtttg
     4081 caaccgcctg gaaactggta gcgcaagcgc ggctgacgct gtaagcatcg gtatgcacat
     4141 tcattccggt accgagaaca atttcacgcg caatgttggg ggcttcaggc atttgtacga
     4201 cctgaccaaa gaccagctgt tcaatcactt cagcggggat ctcgctgcgt gccagcagtt
     4261 cgcctaccac catcttccct aaatcaaccg cgggaatgcc atgaaaagcc gtcgcctggc
     4321 gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg atcgccctgg cgggtaacca
     4381 gcggtaaaac ctgacccata acactcccct gtaaaaaaat aaataaagtg gtctgacctg
     4441 atcatagtct taactatttt tttacattta gccaagcgga gaaaaaggaa agtgggagct
     4501 atgacacagt gaagacagga gaaagaaaag aaaacgcccc tgccagcaac tgacaggggc
     4561 gaacgtaccc gagaggaatt aacgcagacc cagctggaag atcagcattt cagcttcgca
     4621 ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac tcttcagtga aagtcgggga
     4681 gattttgcac ggttcggatt ccacgctacg ggctttttca gtcagcgccg ccagcgtttg
     4741 ttctgcttct gcgcggtttg caaacacgcg gctgtaagac gcggtgcagt cggagttgtc
     4801 cataatggtg ccaacatcca tacagcagca aaccggggtt tcatcagcac tacatttact
     4861 catcgttgat ttcctctgta tgtgcaccca aggtgccaga taaacgttgt ggatatttta
     4921 cgcttccgga aagtgctgct ccagttgtta attctgcaaa atcggataag tgaccgaaat
     4981 cacacttaaa aatgatctaa aacaaaattc acccgaatcc gcgagtgcgc aacctccaaa
     5041 ttttgccagc tggatcgcgt ttcttagatc atatttgaaa aaagatggaa acatacttgc
     5101 agcattccag ctggtccgac ctatactctc gccactggtc tgatttctaa gatgtacctc
     5161 agaccctaca cttcgcgctc ctgttacagc acgtaacata gtttgtataa aaataaatca
     5221 ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa gtctgctctc gcagtcgcag
     5281 tggcacttat ctccacccag gcctggtcgg caggctttca gttaaacgaa ttttcttcct
     5341 ctggcctggg ccgggcttat tcaggggaag gcgcaattgc cgatgatgca ggtaacgtta
     5401 gccgtaaccc cgcattgatt accatgtttg accgcccgac attttctgcg ggtgcggttt
     5461 atattgaccc ggatgtgaat atcagcggaa cgtctccgtc tggtcgtagc ctgaaagccg
     5521 ataacatcgc gcctacggca tgggttccga acatgcactt tgttgcaccg attaacgacc
     5581 aatttggttg gggcgcttct attacctcta actatggtct ggcaacagag tttaatgata
     5641 cttatgcagg cggctctgtc gggggtacaa ctgaccttga aaccatgaac ctgaacttaa
     5701 gcggtgcgta tcgcttaaat aatgcatgga gctttggtct tggtttcaac gccgtctacg
     5761 ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca gttggttgct ggtcagatta
     5821 tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt ggcagctacc gccaacggta
     5881 tcgacagtaa taccaaaatc gctcatctga acggtaacca gtggggcttt ggctggaacg
     5941 ccggaatcct gtatgaactg gataaaaata accgctatgc actgacctac cgttctgaag
     6001 tgaaaattga cttcaaaggt aactacagca gcgatcttaa tcgtgcgttt aataactacg
     6061 gtttgccaat tcctaccgcg acaggtggcg caacgcaatc gggttatctg acgctgaacc
     6121 tgcctgaaat gtgggaagtg tcaggttata accgtgttga tccacagtgg gcgattcact
     6181 atagcctggc ttacatcagc tggagtcagt tccagcagct gaaagcgacc tcaaccagtg
     6241 gcgacacgct gttccagaaa catgaaggct ttaaagatgc ttaccgcatc gcgttgggta
     6301 ccacttatta ctacgatgat aactggacct tccgtaccgg tatcgccttt gatgacagcc
     6361 cagttccggc tcagaatcgt tctatctcca ttccggacca ggaccgtttc tggctgagtg
     6421 caggtacgac ttacgcattt aataaagatg cttcagtcga cgttggtgtt tcttatatgc
     6481 acggtcagag cgtgaaaatt aacgaaggcc cataccagtt cgagtctgaa ggtaaagcct
     6541 ggctgttcgg tactaacttt aactacgcgt tctgataacg cgttcgcctg gataaagtca
     6601 cctgcatagc aggtgacttt aactccccca cttcaccgaa agtagtgtcc tcattgctta
     6661 cctcctgagt tttgcaaaca gcctgttggc agcttgccct ttcaaatcaa taagcggtat
     6721 ccacagacaa acctgaaggg aaaggcatat tttcaggcgt tctgctcgtc cttctcaaag
     6781 agtttacttt tctgcatttc caggatac
//
LOCUS       DAZPBM010000197         6740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPBM010000197 DAZPBM010000000
VERSION     DAZPBM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.198"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            269..454
                     /locus_tag="ACHUZQ_003743"
     CDS             269..454
                     /locus_tag="ACHUZQ_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027340.1"
                     /translation="MKKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSACV
                     QYLINQYMILQGGGNEE"
     gene            444..746
                     /locus_tag="ACHUZQ_003744"
     CDS             444..746
                     /locus_tag="ACHUZQ_003744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027341.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            801..1355
                     /locus_tag="ACHUZQ_003745"
     CDS             801..1355
                     /locus_tag="ACHUZQ_003745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1027342.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            1477..1938
                     /locus_tag="ACHUZQ_003746"
     CDS             1477..1938
                     /locus_tag="ACHUZQ_003746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027343.1"
                     /translation="MAKTYSTFDNRNIKALISQYKLYKDIPCEDIKNIMKDSVAKLKV
                     RDKISVYDVFAVLSNEDELRNDLLFNTINAIKVQQNKKQVKERMIRYYGAAITDAAKR
                     IEQYMLAHPNALPAPVYGQRTLTYKEFGKLLELQKQGDFIDDMIEYLKGLR"
     gene            2402..3145
                     /locus_tag="ACHUZQ_003747"
     CDS             2402..3145
                     /locus_tag="ACHUZQ_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1027344.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            3228..3701
                     /locus_tag="ACHUZQ_003748"
     CDS             3228..3701
                     /locus_tag="ACHUZQ_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027345.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHKIPNSHRLIALLKQDKKT"
     gene            complement(3958..4626)
                     /locus_tag="ACHUZQ_003749"
     CDS             complement(3958..4626)
                     /locus_tag="ACHUZQ_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027346.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASLKSELQSLTFWKVSPKDEAIFNNY
                     FFSNQIALGTLREAISFDINDIHDRYHYNVSYGKLLVDTFDSLIEAHATLKKDMNNLF
                     VFSN"
     gene            complement(4759..5070)
                     /locus_tag="ACHUZQ_003750"
     CDS             complement(4759..5070)
                     /locus_tag="ACHUZQ_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027347.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
     gene            5263..5814
                     /locus_tag="ACHUZQ_003751"
     CDS             5263..5814
                     /locus_tag="ACHUZQ_003751"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027348.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
BASE COUNT         2250 a         1168 c         1364 g         1958 t
ORIGIN      
        1 ctatgatttt agtactattg ctttccccat taaaaatcaa atcgataaat aaatacgtag
       61 taagaaaaat gaaacgctaa aagttttggt gctatactaa tagtatcaga acgataatgc
      121 gttttgcctt ccgctccagc tttgaacata aataatatag cgacaataaa tttccttttt
      181 aattaagatt gcctctcttg cgagagaggt atctttttaa tttggattta taaatacagt
      241 atacaaatac acctataagg atatagaaat gaaaaaagta actttaaagg aacgcaaaca
      301 tcatgtagta accgcacgtc ttaatgacga tcaagtactt gtacttaacc agctaattag
      361 tagtgggaag gtacagaaag attcattatc agcctgtgtt cagtacttaa ttaatcagta
      421 catgatttta cagggcggtg gtaatgaaga atgactttga tttagtacaa tattttgatg
      481 attacacaat agtaggggat atggattatc actatttcta ttgtgtgaag tatcaggaag
      541 gttcattaga agcacattat gaacgaattg gtgatccaga agtattaggt actattacat
      601 ggaatggaaa ttacttaatc gacggattac caatggtaat gtttgtactg gctgctgaag
      661 atgaacataa ggaatgttta aaccgtttgc taatcgtatg tgagaaggcg agggatgaat
      721 ataggctaaa tgctactggc ctctaagtag tagcagacgc gatcacatgg agtgacgcga
      781 taacaacaag gacgtttgat atgaacccta aaatcacttg gagattaatt ccaaattcaa
      841 aacattacta cgtatctgaa aatggcgatg tttacaatga taaaacaaaa cagtatatag
      901 ctacgaaaaa cccatctaat ggatacaaaa aagtttctat taatggcaaa tcactatatg
      961 tacatgtttt ggtttgtact ttattcaatg gtgctaaacc cattggagga agatacactg
     1021 tagatcatat cgaccgcgat aaaatgaata acaaagcaaa aaatttacgt tgggtaactc
     1081 agaaggttaa taattccaat agaaagcata aaggtactcg tactcaaaag cttactctaa
     1141 agcaatggtt aatgattgtt ggagagtacc ttaaaggaca attatctcaa tatgcaatca
     1201 ctaaatggtt tttaaatcaa actggtatta atatttgccc tcagaatata ggtaagttat
     1261 tcaaaggtac tacctattca aaatggtaca caacatatct tacgaataag caaagacaaa
     1321 gaatacaaag cctaaccatt caacgtaaac cataaaaaat gccagtattc cagtactggc
     1381 atctattcaa aactcaatgg caatgaagcc aaacttatta aatggcctgt aatcactacg
     1441 ggtcacgatt aattaattta tacaggatta tttatcatgg caaaaaccta ttcaactttt
     1501 gataacagaa acataaaagc actaattagc cagtacaagc tatataaaga tatcccatgc
     1561 gaagacatca agaacatcat gaaggactca gtagctaaac ttaaagtgag agataagatt
     1621 agtgtatatg atgtgtttgc agtattgagt aatgaagatg aattacggaa tgatttacta
     1681 ttcaatacta tcaatgctat taaagtacaa cagaataaga aacaagtaaa agaacgtatg
     1741 atcaggtact atggggcagc gatcacggat gcagcaaaac gaatagagca gtatatgttg
     1801 gcacacccaa acgcactgcc agcaccagta tatggacaaa gaacactaac atataaagag
     1861 tttggtaaac tattggaact acaaaaacaa ggtgacttta ttgatgacat gatcgagtac
     1921 ttaaagggct tgcgttaatt gtaacttatt acggcatatt acggcatgga tgtatgccgt
     1981 aatacggcaa aaaaagcccc tgaaacccgc tatttaccat caatacggca actaagtata
     2041 gagatacgaa atccattcca tttcattcca tgtatttctt tctctatcag tattccaaga
     2101 aactgtgact tgcgtcacga atactactcg tcgtgaactc ctcgtagtat tggttagtaa
     2161 cgttgcgttt ttctcgccag tactcgaaaa cccgcaaggc gttactactc gctttgctcg
     2221 atagatgtta gtacagcaaa tgaagtacct aaagtgaatt ggtaagtacg acatgccccg
     2281 ccagttccac aactctcgct ctggtcattt ccactactat gatgctattg gatagctaca
     2341 taaagtactg gatcaagtag gcattttttg tgtacaaaga gggaattaag agagggatac
     2401 catgaacaga aaacaattca tccagtcgca tggtgctact tgtagtaact ggacttggag
     2461 ttggtcattt gtgaaccacg acaagaaaat ggttatcttt ggtgcatggg atgtacaaaa
     2521 agagcaagaa agatcagtca ttctgaggga gcggtggaaa accaaagttg tggagggaaa
     2581 ggaaagaaaa aatcttggat atattcaagc tattgaacac attcaattga tagctgaagg
     2641 ttatgagctt tacacgtttg atatggagca gggacgaaac gatgagaata gagatgtagc
     2701 tgttatcaaa cggttcactc caaaacttga aaaacgttac cttcggaaag aaggaacagt
     2761 ttggtatgca gactttttac ctaatccatt tccagatgaa attacctcac ctgaaaacta
     2821 cgctgagggt gcaaaaaagc aagttacagt aaactcctac gagcgtgatc caaaagcacg
     2881 acaggcttgt attgaccatc atggtacttc ctgcgggtgt tgtgagttta actttgagaa
     2941 agtctatggt gaacacgcca agggctttat acacgttcat catatcaaac cgctacacac
     3001 agtaggcgag gattacgagg taaatccaat tactgatatg gttccacttt gtccaaactg
     3061 tcacgcaatg gtacatcgcg gtaacgaagt attaagtgtc gaaagtttaa aagcactgtt
     3121 gtttaaagac agcaataaaa tttgaattta ttttagactt gatacgacat acttgaaaag
     3181 ttatgaatac aatatgcttt aaattagaca tgtaagttga gagaaaaatg agcataacaa
     3241 caatagcagc tacatataaa gatgtgttaa ctattgcttt ttcggcgttg tcatttatta
     3301 ttgcatgtac ttccttatac ttctccggta gggcagcatg gcatgataga tcaaggctaa
     3361 aaataacagc tgacctcgtc tacgagtctt ttgatgagga accttataaa atcgaggtga
     3421 tagtgatcaa tgttggtagg cgtgatgcag ttttggaagg gatgctttgc cattatgaaa
     3481 aaggcattag gagccattca tctccaaaag agagcattgt tattaaagaa aagcaaagaa
     3541 aaaaatttga attggtaagc tcagacttag tcacgataga taatgaatgt gatatttgtc
     3601 atttagaaga tataactatt ttagatgtgg aggggaatga acataaaatc cctaactctc
     3661 acagactcat tgcattactt aagcaagata aaaagactta agaataatat tttttggaat
     3721 gtaaggtaaa tgttagatgc cactatagat tgagggttag aactactaac ccactatagg
     3781 gtttacgaga tgataagaat ggaatttaac acctacgatg gtgatatata ttacttcctc
     3841 tatttatata ggaggtacga aagggggagt actttaatga agaccttata aatcttaatg
     3901 ttgagatgtt agttatagcg gtgaggatac cccaccgtct attgggagtt gtggactcta
     3961 attagagaaa acgaataagt tattcatgtc ctttttcaag gttgcatgtg cttctattaa
     4021 actatcaaat gtatctacta ataactttcc atagctgaca ttgtaatgat atctatcatg
     4081 aatgtcattt atatcaaaac ttatcgcttc cctcaatgtt cctaaagcaa tttgattact
     4141 gaagaaataa ttattgaata tagcttcatc ttttggcgat actttccaaa aggtaagtga
     4201 ttgcaattcg ctttttaatg aggctaactt aatgtttaat agatcgataa cttctcgttc
     4261 agttttaact aaatgtgcga aattttttgg ttctatatct ggttcagtat tgttaaggtt
     4321 gcatatattt tcatatgttt tcaacatctc caacgaaaaa agatcgcaat tagtccagaa
     4381 cttagtaact tgatttaaag cattttcctt tattttagtt tgaaaccaat ttttagcgtt
     4441 ccatgctgca tatagggctg ctccagccat aatgagattg gcaatagaac taacccagtc
     4501 actaaccgat ccccactcga aaccttcaga accaacaaag agtgatttta ttagtactaa
     4561 aattacaaca accagtaata ctgctacggt cgataaaaga acgatattta attttctaca
     4621 atccatcgaa agtagttcct gaacgcatta aaggggcaaa tactttacca tccgaaacac
     4681 aatcaagtac actacccgtt cacgtactgc tcacatgttg ggtttgttat tttgttgggg
     4741 tgagcagtac gtgtatcctc agaagggtag ttccccctga tcacagaacc aatcaccttc
     4801 ttcactgctt tgattcatcc ggtactcagc agatagatat gctaacccat caataacact
     4861 tattagtggt gtttcacagg taagtacaaa acaatctctc tctatagtac ctaaccagta
     4921 ttccctgtac tgattctgga agaataccct atccccataa tgaaattctg ttagtgcaga
     4981 tccccaataa cacacggttg cccagcgacc gttatgtgag aacttctgaa cccctcgcgt
     5041 aatggatgga tcatagccgt ctttgctcat ttgttaccct cctctatgta ttactgtata
     5101 tatgtacagt attgcagtca gtttcccaaa aatccagcac ccctttgttt accactttga
     5161 atgattaatt ctattgtaat gatcaataaa attgatcgat ttaattgcca atgttttaaa
     5221 aatgatcaat tatcaataac ttaaagcgta acgagcaaag ttatgaacat tgataattgg
     5281 gcaataccga taacaagttt gggtggtcag ttgctggggg ttgagttggt cagccatgta
     5341 gaacgcgagg gaatcaggca gatcggccta tgggaagaag aagtacttca tgggcaacta
     5401 atgcttattg aaagcaaggc ggcatggttc agggataacg gtttgtattg tgtgattact
     5461 actggccgtg gtgaaccata cgagtactta ccattccttc ggcagatgac aagggatatg
     5521 aaagtacata accagcagtg gcttgatgat ctcggtgtac atggtggttc tgctattcct
     5581 ctggttacag gtgatttcga ggttgcccga ttaaatagaa ggtacacaga agagaacatt
     5641 gaccgtctga tctttccagt actcataaaa agcattcgcc agtactgcga gaaggtgatc
     5701 gtacccgtca gagataaaac gtattaccca attctcagag aggccggtgt atgggcggta
     5761 cagggagagt tcaggccaat gcgttttgag caatgtgaaa aattaatagg ataacaataa
     5821 tgaaaaaatt actaattggg ttactgattg cccttagtac tggatgtact actacgcaca
     5881 gcttcatgga taaagcttac gttggctatg tagatgacaa ggccgtagta ctgtttgatg
     5941 caggaacacg taatggggtg aagtactacc gtcctcagtt ccagagtgat tgcggtcgca
     6001 tctataacaa gatctatcat gatgacaata caagtactgg atgcctcata cgagattccc
     6061 gcattgctga actacatccc tatagagagc atgtaagtaa ggtagtacca catcagaccg
     6121 atagtgttca cgattggtta gaaggggtca attcaccatc ccatatcctc gacaatctat
     6181 agggtacgta tgggaaaggt aatggtgatt ttgttagtac tggctggggt actcttcaac
     6241 attgcaggtg aggatgcacc ccagccacag tacacggaca atgagaacca gaacgctatg
     6301 gatttcgcac agtcactaat caagggtaag tacccatgcg atatggtgac tatggatagt
     6361 actgagaatg gtacaggacg tattgatgta cggtgtgatg gtaagtacca gtatgagatc
     6421 atccggcatg atggacaatg gcaggtagta tcgaagtaat ccgaaaagtt tagtgcttag
     6481 gctgtatggt gagaatctac agggcttttt ctttatgcgg tgataagatc ggagtgaact
     6541 ttttatgaag gaatagttag tagaatgaaa attacagtcg ataatatctc tgaaatgttt
     6601 gcaggaacta aagaactact aatcggtggc ttaataacag gtatcgtcgg agtacttatt
     6661 ttaactcgtg ttagtggtta tgaatacgtt aaagtacctt taatgttagt aatgatattc
     6721 ttactaactg tagttcctgg
//
LOCUS       DAZPBM010000198         6730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPBM010000198 DAZPBM010000000
VERSION     DAZPBM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.199"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1094)
                     /locus_tag="ACHUZQ_003752"
     CDS             complement(<1..1094)
                     /locus_tag="ACHUZQ_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1027349.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
     gene            complement(1131..2576)
                     /gene="abgB"
                     /locus_tag="ACHUZQ_003753"
     CDS             complement(1131..2576)
                     /gene="abgB"
                     /locus_tag="ACHUZQ_003753"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR1027350.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(2576..3886)
                     /gene="abgA"
                     /locus_tag="ACHUZQ_003754"
     CDS             complement(2576..3886)
                     /gene="abgA"
                     /locus_tag="ACHUZQ_003754"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR1027351.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            4062..4970
                     /locus_tag="ACHUZQ_003755"
     CDS             4062..4970
                     /locus_tag="ACHUZQ_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1027352.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            5300..5863
                     /gene="smrA"
                     /locus_tag="ACHUZQ_003756"
     CDS             5300..5863
                     /gene="smrA"
                     /locus_tag="ACHUZQ_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR1027353.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQVKQENWERHAKRSR"
     gene            complement(5884..>6730)
                     /gene="dgcM"
                     /locus_tag="ACHUZQ_003757"
     CDS             complement(5884..>6730)
                     /gene="dgcM"
                     /locus_tag="ACHUZQ_003757"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR1027354.1"
                     /translation="FYARFFLTNSAPMLLIDPSRDGQIVDANLAALNFYGYNHETMCQ
                     KHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQTYAGPIEIYGDKL
                     MLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLL
                     IDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDT
                     ALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRVDDALYRAKNDGRN
                     RVLAA"
BASE COUNT         1685 a         1715 c         1704 g         1626 t
ORIGIN      
        1 atgttgctcc agttaaacat ggcgacaaac tgggcgaggg gaaaaaccat cacgataaat
       61 cctgccatct ctttcatcgg ttcaatcatt aactgcggta aatccgcctg acgtcgaatt
      121 gtgcgggtag cgatgccata agccagcgag acaacaaaga aaaaaagaat gatcagtggc
      181 acgatacctt taataaaggg tgatggcatc acggtgtgat taatcggatc gcgcaatatc
      241 ccgttttccg ggatcaccat cagcgcaatc gcagcaataa aaagtagcga tacgacacct
      301 gctatgcgta aaccaaaacg ctgactttcg gtcaatgtct gcagtttctc atcgctgttt
      361 ccctgccatt gacctaaccg tggctcgatg attttgtcgg ttatcaggcc gccaacaatc
      421 gtcagtacga ctacggagct ggccataaaa taccagttat caattacact gacgtgcatt
      481 tgcggattga acgcagccgc cgcttccgtg ctgatccccg acagcaacac gtcggttgtg
      541 acaatcagta aattagccgt aaagccgcaa cctacgcctg caatggcagc cagtaaacct
      601 gcaactggat gcctgcccac cgccagaaaa atcagcgcac ccatcggtgg catgatcact
      661 aacgtcgcat cggaagaaat gtggctgaaa aaagcaataa acagcaccat ataactggcg
      721 tagcgggcat taacatgcga tgccatttta accattagcg ctggcagtaa gccgacgcgc
      781 tccgccagac cggcacctaa aaccagcgcc aggatcgcac caagtggagc aaaaccgcta
      841 aagtttttaa taacattggg taaagaccag tgtaatcctt ccacactgag caggtttttc
      901 accaccaccg gcgttccatc ggtcgggttt ttcgcactga cgccaaaggc cgacaaaatt
      961 gccgtcgtca ccatgagtac gataatcaaa tagataaaga gcaaaaaagg atggagaacc
     1021 ttgttaccaa ttctttcgac ccagccatag agcttcccgg attgggagga cgacggtatg
     1081 gatgacatac tcatgggcat tcctcggttg ttgtgtttgc gttgttgttg ttattttaaa
     1141 ggtgacggtg tcacgttttt cgggataggg cagtgatacg gttgcgtgtc cgttacttgc
     1201 tgatgctctt gttggcattc ttgcaatagc cctgaatcaa tgaagagatt gactgtggtt
     1261 gctgccatag ttttcgccgc cagcagcatt cctttatgag caatagatgt tcgcccctgg
     1321 ctaaccagtt gccacgtatg tagcggtgta ccgacggcaa aacaggggct gaaacactgg
     1381 gcaacaggca gtttccaact aacgtcgccg acatcagtcg atgccgcaag cacgttatcg
     1441 gtggcggcat atggagcgac ctcattcgcc agtaccgttt cacgatgacg tagtgcaaaa
     1501 gccttgccgt tttcgccacc ggttgcggcg atattattca gactgttttg ccgatcgttg
     1561 ggggtgagcg tagcctgaat ttgtttcgca aaagccagtt cttcggagtt ccattccggg
     1621 gtaccaaaat gggatagggc atggtacatg gcattttcta aggtgcgatt cgggagataa
     1681 ctggaacagg ctttgtcgaa gcggcattca accgtggttt cggtcatcaa tgccgcacct
     1741 tcggcgattt tggcgacccg atcataaata tgctgcacgt cggtcatttc gggggcgcgg
     1801 ataagataaa gcacttctgc ctgcgcctgg accacgttgg gcgagatccc gccgctattt
     1861 gtgatggcat agtgtacgcg cgctttttca ataatatgtt cgttgaggaa gttggtgcca
     1921 gtggtcatca acgttacggc atcaagggcg ctgcgtccca aatgagggga attcgcggca
     1981 tgtgctgcga tccctttaaa gcgccatgat gcctgaatgt ttgccagcgt gcgggtattg
     2041 aacataccgg caaaggcttc cgggtgccag gtgagtgccg catccacatc atcaaatacc
     2101 ccctcgcgga ccatgaacgt tttacccgag ccgccttctt cgccaggaca accataaaag
     2161 cgcactgtgc cgccttgccc atattgttcc agccatttct tgacggctat tgcagcggca
     2221 aaggcggcgg ttccccgcaa attgtgtccg caaccgtgac cattttcacc gggcgtcgcg
     2281 gatgtaggtt gcgcgcaacc agcttgctga cttaaacctg ccagggcgtc atattctccc
     2341 agcagggcga taaccggttt gccttgacca aacgaagcaa taaaggcatt tgggatattg
     2401 cctacgttgc gggtaacggt gaagcctgca gattccagag ccgaagccag atgctccgct
     2461 gaccagaact cttcaaaacg tgtttctgga tgatcccaga tttgatcggc aatatcggta
     2521 taacgttggc gatcggcttc aatcgcatcg tcgataaaac gatagatttc ctgcatcaga
     2581 tacctcgcgt ccagggaaaa ttgagcgcgg tgcgcgccag cgtttcgacg gcaatagcga
     2641 gaacctgctc gtcaaaatcg aatttttcgt tgtgatgacc tgccgccagc tgtgtgccaa
     2701 acaccacgta ggaggcttgc ccttgatgtt gctgcacgcg ggccatcatt aatgtggcat
     2761 cttcggaacc cgcaggcgct tcaacacgtt caatggcctg attgaccccc gcgacctgag
     2821 ccgcctgact ttgcaaccat gcgacccatt gcggcgaagg agaactggcg gtagctgcac
     2881 ccatcagacg agtttcaacg ccgacaccat acatggttgc tgcgccctga atcgcttgtt
     2941 gtgcacggtc aaaaacatat tgattaatga cgtcgctggc cccgcgtgtt tccactttca
     3001 gcaacgccga ggcaggaaca acgttacgac cgcttcctgc ctgcataacg cccacgttta
     3061 ctctggaagc tccttcgctg tgcggggcga ttgcatgcag tgcaagagtg gcttgtgctg
     3121 ccgccaacaa ggcattgtga ccgtcttctg gttttgcgcc tgcgtgagcg gcggtaccgg
     3181 tgaagtgcgc gtcaaatttg gtggttgcca taaaattatc actgccgcac accacggtgc
     3241 ccgcaggtac gccagtgcca atgtgcacgg cagtaaaata atcaacatca tctacgacac
     3301 ctgcatcgac catcgcccgc gcgccacgcg taccttcctc tgcaggctga aaaatcagtt
     3361 tgatgacgcc atgtagtccg gactcgaatt gtttaagggt atgcgccagc ccaagcccaa
     3421 tggcggtatg tccatcatga ccacaggcat gcatcattcc ggcgttacat gacgcaaaac
     3481 cgtcgcggta ggggcgatgg ctgacatcct gctcttcact gagatccagc gcgtccatat
     3541 cgacacggaa agccatcacc ggaccggggc gaccggtatc cagggtggcg acgataccag
     3601 tgaaaccacc ttcaaaaacc gcaatccatt gtgctagcgc accctgttga cgagcgcgct
     3661 cgaattcgcg ttgtagagtg agttcatcag gtagtcccat ccggctactt tcattaacta
     3721 cttcgcggcc cagcgccagt gaatagccga gctgctgcaa ttcttccgca acaagggtgg
     3781 cagtgcggaa ttccacccag ccagactctg catagtgatg aaaatcacgt cgccagtgcg
     3841 ataattttgg ggcaagcgaa ttaacaaatt gattcaaaga ctccataacc tttcccgtca
     3901 tcagtaaaaa gtgtgacccg gttcacgtag cgatagtttt tacttatcac taactgattt
     3961 ttcacagttt taaccgttca taaattaccc tgacacaatc atctgcatta aagtagatgc
     4021 cagtttcttt ggtctgataa ataacggtta tcggtggcgt catggctttt caggtaaaaa
     4081 ttcatcaaat tcgggctttt gttgaagtgg ctcgtcaggg cagcattcgc ggagcgagcc
     4141 gaatgttgaa tatgtcgcaa ccggcactga gtaaatctat tcaggagctg gaagaaggat
     4201 tagcggcgca actctttttt cgccgtagta aaggcgtgac gttaactgat gccggtgaaa
     4261 gtttttatca gcacgccagt cttattcttg aagagctgcg cgcagcccaa gaggatattc
     4321 gccaacgaca agggcaactg gcagggcaga ttaatatcgg catgggggcc agtatttccc
     4381 gcagtctgat gccagctgtc atatctcgtt ttcatcagca gcatccgcag gtaaaagtac
     4441 gcattatgga agggcaactg gtgtcgatga ttaatgaatt gcgtcaggga gaattggatt
     4501 tcaccatcaa tacctattat cagggaccgt acgaccacga atttactttt gagaaattac
     4561 tggaaaagca attcgcgatc ttttgccgcc cgggacaccc cgccattggt gcccgttcga
     4621 tcaaacagtt actggattac agctggacaa tgccgacgcc acacggcagt tactacaaac
     4681 agttgagtga attgcttgac gatcaggcgc aaacgccaca ggtcggtgta gtctgcgaga
     4741 cgttctcagc ctgtatcagt ctggtggcaa aaagcgattt tctcagcata ctgcctgaag
     4801 aaatgggctg cgatcccttg cacggacagg ggctggtgat gttgccggtt agcgaaattt
     4861 taccgaaagc ggcctattat ttgattcagc ggcgtgatag tcgccagaca ccactgaccg
     4921 cgtcattaat cacgcaattc cggcgagaat gcggctatct gcaaagttaa aactgcataa
     4981 aaaaatagag tctgtcgaca tccgccagac tctacagtac acacagcagt gcatccgcgt
     5041 cttaaatccg gcattgtctc ctctgcgccg gtgactgtgt tataacggtt ttaaccttta
     5101 gttgccaatt ttcttcagtg acagatttca cgaaaatata agttacatta ttaatatcgt
     5161 gaatgaataa tcatgcataa gcattttgct taaaatatcg gcaatatttg gaacttatta
     5221 ctggaatttt gggtaatacg ttgttggacc gacccggtct ggttatcata tcgcgctctt
     5281 aattgcggga ggatgtaaca tgaaccttga cgacaaatcg ctgtttcttg acgccatgga
     5341 agatgtccag ccgctgaaac gtgctaccga tgtccactgg catccaacgc gtaaccaacg
     5401 tgcgccgcag cgtatcgaca cgctgcagct tgataatttc ctcaccaccg gatttctcga
     5461 catcatccca ctaagtcagc cgctggagtt tcggcgggaa gggttgcaac atggggtgct
     5521 ggataagctg cgcagtggta aatatccgca acaggcgagc ctgaatcttt tgcgccagcc
     5581 ggtggaagag tgccgcaaaa tgatgttcag tttcattcaa caagccatgg ctgatggttt
     5641 gcgtaacgtg ctgattattc atggcaaagg acgggatgat aaatcgcatg ccaatattgt
     5701 ccgcagctat gtggcgcgct ggctgaccga atttgatgat gttcaggcat attgcactgc
     5761 gctaccgcat catggcggca gcggggcgtg ttacgtcgca ctacgtaaaa cggcgcaggt
     5821 gaagcaagaa aactgggagc gccacgctaa gcgcagtcgt tgatctcgag acgcatccgc
     5881 ggcttatgcc gccagcacgc ggttgcgtcc atcatttttc gcccgataca aagcatcatc
     5941 aacgcgttta aacagttcat cgatgctttc atttccttcg tgatgcgcca caccaatgct
     6001 gacggtaaag cgtggtaagc ccgaaatact cacttttgcc acgcttacgc ggatagtttc
     6061 agccagcgaa agcgcggtat ccagtggggt tcttggtagc aataagacaa actcttcgcc
     6121 tccccaacga aacaccaaat cgcctttgcg agcgcaactt tcgagggtgc gggcgagggc
     6181 gcataacacc tcatcacctt tagaatgccc atagagatcg ttaatgtgtt taaaacgatc
     6241 ggtgtcgatg agcaacaagc tgtaatcttg ggcgatggcg agatgctgca tttggcctgg
     6301 ctcggtaatg tgataaaact gtcgccgatt cagtaatccg gtcatcgcgt catggtgggc
     6361 agcatgttcc agctgctcct ccagccgttt ttgctcagta atatcatgta caatacataa
     6421 catgagcttg tcgccataaa tttcaatcgg cccggcgtag gtctgcacat gacgagtcga
     6481 accatccgcc agcttatgaa taaaattcag aggtttatga ccaccgggta aatgcgaaat
     6541 ttcatgcatg ataggcatga cgcgacgccc gagcatattt atttcccagg tatgtttctg
     6601 gcacatcgtt tcatggttat aaccatagaa attgagcgcg gcgaggttag catcgacgat
     6661 ttggccatct cgtgaggggt caatcaacaa cattggtgca gagttagtca gaaaaaagcg
     6721 cgcataaaaa
//
LOCUS       DAZPBM010000199         6703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPBM010000199 DAZPBM010000000
VERSION     DAZPBM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.200"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            17..153
                     /locus_tag="ACHUZQ_003758"
     ncRNA           17..153
                     /ncRNA_class="other"
                     /locus_tag="ACHUZQ_003758"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(244..930)
                     /locus_tag="ACHUZQ_003759"
     CDS             complement(244..930)
                     /locus_tag="ACHUZQ_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1027355.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1511..2299)
                     /gene="phoH"
                     /locus_tag="ACHUZQ_003760"
     CDS             complement(1511..2299)
                     /gene="phoH"
                     /locus_tag="ACHUZQ_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1027356.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2920..4191)
                     /gene="efeB"
                     /locus_tag="ACHUZQ_003761"
     CDS             complement(2920..4191)
                     /gene="efeB"
                     /locus_tag="ACHUZQ_003761"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1027357.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4197..5324)
                     /gene="efeO"
                     /locus_tag="ACHUZQ_003762"
     CDS             complement(4197..5324)
                     /gene="efeO"
                     /locus_tag="ACHUZQ_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1027358.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5382..6212)
                     /gene="efeU"
                     /locus_tag="ACHUZQ_003763"
     CDS             complement(5382..6212)
                     /gene="efeU"
                     /locus_tag="ACHUZQ_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1027359.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         1726 a         1795 c         1545 g         1637 t
ORIGIN      
        1 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
       61 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
      121 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
      181 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
      241 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
      301 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
      361 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
      421 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
      481 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
      541 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
      601 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
      661 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
      721 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
      781 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
      841 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
      901 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
      961 atttcattac caatgttact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     1021 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     1081 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     1141 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     1201 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     1261 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     1321 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     1381 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     1441 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     1501 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     1561 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     1621 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     1681 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     1741 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     1801 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     1861 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     1921 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     1981 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     2041 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     2101 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     2161 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     2221 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     2281 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     2341 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     2401 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     2461 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     2521 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     2581 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     2641 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     2701 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     2761 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     2821 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     2881 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     2941 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     3001 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     3061 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     3121 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     3181 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     3241 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     3301 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     3361 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     3421 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     3481 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     3541 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     3601 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     3661 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     3721 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     3781 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     3841 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     3901 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     3961 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     4021 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     4081 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     4141 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     4201 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     4261 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     4321 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     4381 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     4441 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
     4501 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
     4561 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
     4621 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
     4681 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
     4741 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
     4801 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
     4861 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
     4921 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
     4981 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
     5041 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
     5101 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
     5161 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
     5221 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
     5281 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
     5341 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
     5401 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
     5461 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
     5521 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
     5581 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
     5641 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
     5701 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
     5761 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
     5821 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
     5881 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
     5941 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
     6001 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
     6061 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
     6121 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
     6181 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
     6241 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
     6301 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
     6361 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
     6421 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
     6481 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
     6541 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
     6601 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
     6661 caggacgtta cctcgcgccc gacccggtag ccgaagggga taa
//
LOCUS       DAZPBM010000200         6651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPBM010000200 DAZPBM010000000
VERSION     DAZPBM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.201"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..541
                     /locus_tag="ACHUZQ_003764"
     CDS             <1..541
                     /locus_tag="ACHUZQ_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR1027360.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
     gene            complement(597..1139)
                     /locus_tag="ACHUZQ_003765"
     CDS             complement(597..1139)
                     /locus_tag="ACHUZQ_003765"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1027361.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1123..1434)
                     /locus_tag="ACHUZQ_003766"
     CDS             complement(1123..1434)
                     /locus_tag="ACHUZQ_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1027362.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHUZQ_003767"
     CDS             complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHUZQ_003767"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1027363.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2521..2850)
                     /locus_tag="ACHUZQ_003768"
     CDS             complement(2521..2850)
                     /locus_tag="ACHUZQ_003768"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1027364.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2850..3464)
                     /locus_tag="ACHUZQ_003769"
     CDS             complement(2850..3464)
                     /locus_tag="ACHUZQ_003769"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1027365.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHUZQ_003770"
     CDS             complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHUZQ_003770"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR1027366.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHUZQ_003771"
     CDS             complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHUZQ_003771"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR1027367.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1804 a         1688 c         1646 g         1513 t
ORIGIN      
        1 gggcattatg tacgccaatg gtcagtatgt tgaggtagat tgtaagaaag cgaaggaata
       61 tcttgataaa ggtgtccaca tttatggtgg cccggaagac tttctggcta cttgtcgaaa
      121 agacatgatt gacagaaaaa cggttgacga tacgttaccc gtgattacgg ttacccgttc
      181 tgggatgcga gataattttt tagataaagg tttttcctgc atggatagcc tcttcgccac
      241 caccaataaa ttaggcgaag tggccaatct tcgcgtgaca ttcagcattc gtcgcccatc
      301 cggtaaggag attaaccaaa cggttggctt cgcgcctttc ggtttaaacc ggctgaatat
      361 tagctttact gattacctat ttggttcatt taccagtaat tcgtctctta ttctttataa
      421 accggaattt gagcgaaaat cttgtgcaac tgtaaggacc acaatcgttg ccgcaacggc
      481 aaccattaac ggcaaggatg tggagttgct gaaagcgggg gcaattgaac aaaagtggta
      541 acgctgctca gcccccggct aaggcaaaac ttgccctggc gggtggcgat aataatctaa
      601 gccgcgagaa tatgctgaat cgccaaaaac ataggtacag caccaaaagc atttggtgtc
      661 gctgaaagct gaacaaagcc gagacgagca taaaaattaa tggcggcagg gtcagcatca
      721 agataaaccc ctttaattgg taatgcctgg tgaattattt ttacatgttc aaaaaaatca
      781 catagtaggt cctgaccaaa gccccgcttt tgatacttcc gtgctacccc caacatgact
      841 aatctgacaa caccaatttc tgaaggttgt gaaccctgaa ggacgccaga aacgcgttgt
      901 ttttccagcg aatatgccgt aaaagtacag atgccgatca gttcaccact ttgtctgtca
      961 ataagtgctt tagccgcaca gtcgctatta cgcacacttt tctttagcga tgcgcgtaca
     1021 aatttatcga taacagagtt accgcaatca aactgttttt gcccaggata agtaatatca
     1081 gactgataat tacaggagag gactatgggc agagtaatct cttcatgctt gtcgaccatc
     1141 gctgtttttc cttttcatta acgccttgag ggcgagcgtc ggttgagcag gttcagcgat
     1201 gagttggttc atcagttccc agctttgatt cgaaagctca cggcgacgtt ggttatcaag
     1261 cacactttcg gcgcgttcca tagcagcgct caaaataaaa gcgcttaaat ctaccccaac
     1321 ggcttgcgca gcttcacgaa gtttgttttt ggcgtcggga ctagttttga gttcgacccg
     1381 cgcatttttc agcgttttat cgacatcggg tagagtggct gtttttccag gcattatgac
     1441 ctctttgatt aactgaatga aatcgtaggg agtcgatctc atacataata cggctatcaa
     1501 ccgtatgatt aatttatgtt catctgctca ctctgtcaag acatacggtt attagccgta
     1561 tctctgatct cagacaataa aaaaggtgcc gtagcacctt tttaatagag aggttgtgtt
     1621 accaaacagc agccagcagc gtatgcgagt ccggcaccat aaaatcaacg gacatcatca
     1681 ccgagagggc tgtgatggcg atgatagaga agccgaacag cttgcgcgcc cagatcctgt
     1741 catcagcaac tttataaccg cgcagggcca tacctaacca ccagacgctc accgccgcgg
     1801 cgaccaccag atatttatac ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg
     1861 caaaggcgat gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg
     1921 gcaataccgg aatgtttgcc gcctgataat ccttaaagcg gaaaatggcg atggcatagg
     1981 agtgaggcat ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact
     2041 cgccggttac tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca
     2101 atgtgccgta gaccgagtgg cgtttcatgt acaggctata aacgccgaca taaaccacaa
     2161 agcccatcac ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc
     2221 cagcaaagcc tagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa
     2281 ccagcacccg attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt
     2341 taaacacaca acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg
     2401 gataatcaat gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc
     2461 caaagatgat gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc
     2521 ttagtgcatc atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag
     2581 gatagcgatg attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt
     2641 ggtattcatg tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag
     2701 gattgttccc agaattacgg ccggagaggc agctcctgtc atcaccatcc agaacggaat
     2761 caccgtcaga atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga
     2821 cgcgccgctg tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg
     2881 aacacacaga tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg
     2941 gtacggttag tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag
     3001 ataagaccgg aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg
     3061 aagccgctgc gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc
     3121 atcccgataa atccggcacc aaacaaccag gtcaacgcca gccaggagat aacctggctt
     3181 ttgttgtttt tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc
     3241 aagaaagttt caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct
     3301 gcggtgccgt tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg
     3361 ctcatcaggt agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt
     3421 tcgtgcgcgt gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt
     3481 agtaatctca tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta
     3541 atccacgtcc tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac
     3601 aatcgccagc caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc
     3661 aatgacgata cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt
     3721 tttatacgct tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg
     3781 cacaacggca aagttataga acggaggcgg ggaagaggtt gcccactcca gcgtacggcc
     3841 accccacggg tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat
     3901 ctgaataacg aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat
     3961 cagcatggtg tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa
     4021 gcccagcgca tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc
     4081 gcgtttaccc caggtttcat tcagtttgaa accgaacgct ttaggccacc agtaggtcat
     4141 ccctgcgaag cagccgaaga ccacgccgcc gatgataacg ttatggaagt gcgcaatcag
     4201 gaacaggctg ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat
     4261 cccgcccacc gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac
     4321 gatgcggccc tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc
     4381 gataatcatt gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa
     4441 gaagtggtgc agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag
     4501 cgaggtataa ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc
     4561 gaaaacaggc aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat
     4621 gtacatcatc atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc
     4681 cagggtcaac aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt
     4741 cgcgcacagt gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg
     4801 catcttcaga atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag
     4861 ctggagactc catatccagt aatcgacacc gactcccgga ctgtactcta ttcccgatag
     4921 cggtggatag gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac
     4981 cagaatcaca ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac
     5041 gtcacgcgcg ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat
     5101 cgccacgaag aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg
     5161 tggcaggaag cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc
     5221 gtcagcaaaa ccacgcagca acatcacaat cgccacgatg atatacatga taccgaggcg
     5281 tttatggtcg acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt
     5341 gatcaggcca acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat
     5401 aggttcatgg aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca
     5461 acccctttaa tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc
     5521 actgtgctca ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt
     5581 aatcacatcg gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc
     5641 gctaggcgcg gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgttt
     5701 cgcttttgcg acccattggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt
     5761 catgcctgag aagcccggcc cgctgtagct ggcggagata ccgtcataag taccgggttc
     5821 gttggcgatc agatgcaggc gagtctgcat accggccatg gcataaatct ggctacccag
     5881 acgcggaatg aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt
     5941 gttcgccggg aaagcgattt cattcacggt tgcaatgccc tgttccgggt agatgaagaa
     6001 ccatttccag tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg
     6061 cttgctaggc tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat
     6121 taagataggt accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct
     6181 gtacttagca tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat
     6241 tgcgggaata acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc
     6301 cagaccaatc tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt
     6361 gcctgcaaat aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg
     6421 acctcaattc cacgggatct ggtggcgttt aaagtgtgtg ggcattttac ggggaggttg
     6481 cattactgta aacatgatta aattcctgtt acttggattt ggctgtcagg ttacatatgt
     6541 tgcaaaacat atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg
     6601 gagtgcagaa ggagtttgga cgggcaattg gtataaccaa tctggaataa a
//
LOCUS       DAZPBM010000201         6634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPBM010000201 DAZPBM010000000
VERSION     DAZPBM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.202"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..154
                     /locus_tag="ACHUZQ_003772"
     CDS             <1..154
                     /locus_tag="ACHUZQ_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027368.1"
                     /translation="SEQIFKITGDENVAKNELEPWTPEGNAPNYCWWEVDPVDAANEA
                     MSYHND"
     gene            187..405
                     /locus_tag="ACHUZQ_003773"
     CDS             187..405
                     /locus_tag="ACHUZQ_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1027369.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            476..793
                     /locus_tag="ACHUZQ_003774"
                     /pseudo
     CDS             476..793
                     /locus_tag="ACHUZQ_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(826..1464)
                     /gene="ompW"
                     /locus_tag="ACHUZQ_003775"
     CDS             complement(826..1464)
                     /gene="ompW"
                     /locus_tag="ACHUZQ_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1027370.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1704..1793
                     /locus_tag="ACHUZQ_003776"
                     /pseudo
     CDS             1704..1793
                     /locus_tag="ACHUZQ_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1821..2564
                     /locus_tag="ACHUZQ_003777"
     CDS             1821..2564
                     /locus_tag="ACHUZQ_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1027371.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2594..3133
                     /locus_tag="ACHUZQ_003778"
     CDS             2594..3133
                     /locus_tag="ACHUZQ_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1027372.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3238..3636
                     /gene="yciA"
                     /locus_tag="ACHUZQ_003779"
     CDS             3238..3636
                     /gene="yciA"
                     /locus_tag="ACHUZQ_003779"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1027373.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3676..4395)
                     /gene="tonB"
                     /locus_tag="ACHUZQ_003780"
     CDS             complement(3676..4395)
                     /gene="tonB"
                     /locus_tag="ACHUZQ_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1027374.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4486..6006
                     /locus_tag="ACHUZQ_003781"
     CDS             4486..6006
                     /locus_tag="ACHUZQ_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1027375.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6039..>6634)
                     /locus_tag="ACHUZQ_003782"
     CDS             complement(6039..>6634)
                     /locus_tag="ACHUZQ_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1027376.1"
                     /translation="RAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQKQ
                     IITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATGK
                     TATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVK
                     NGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1730 a         1528 c         1575 g         1801 t
ORIGIN      
        1 ctctgagcag atttttaaaa ttactggcga cgagaatgtg gcaaaaaatg aattagaacc
       61 gtggacacct gaaggaaacg caccaaatta ttgctggtgg gaggttgatc cggttgatgc
      121 tgcaaatgaa gccatgagtt accacaacga ttaatgtcgg gaggccgccc gaaagggcgg
      181 taagaaatga ctacattatt cagaaaagaa tatccgcaaa aaagtagggc gacagaattt
      241 ttgtttctca ttctgtttat cgtattgatg ataccgatat cccctctaat ttttgtctgg
      301 gcaatcggga aaataattga gccagttact gaattgtata ccgacgttgt atgggcgtcg
      361 ttcaacacac tgcacaataa aattaatccg tataaggaaa actgatatgg caactttgac
      421 aaaaaaagaa cgggcatggt tgaacgaatt acaggaagtt cttgatcgct gtcctgtggt
      481 gtcatcctgc cgcgattgcg ttcttagtcc gtggctattg caccaccatc acgcgaaagg
      541 gacagctaag cgcggcggga tggttaagcc agcaacatta accgttgcat ttaaaaaagc
      601 ccgggattct gtggattaca actggcgtgc taatggcacc ccaccctctt tccatgagca
      661 gagatcttta tcagagcgat tgttcagaga gcagggggtt gataccaaaa ttttgctagg
      721 ccattcgaat caaaaaatga ccgatattta caacgatgta cgcggtaagg agtggaaaaa
      781 actggtcatt tgataaccag ttttgcagag gttttgcaga gaaaattaaa agcggtaccc
      841 ggcagagaac ataaacaccc acggatccag tcgtaccgag tcttttacgg tagtaacacc
      901 agatttgtac ttagccgtgg tatcgatatc catgtaccac actgacatgt taaccaacca
      961 gtcacggtta atcagataat caacccccac ctgcccggca gctccccagt aatctttcag
     1021 actgagatcg gaaagccccg cctctttgcc atgatcgtta aatccattat caaagaaggt
     1081 ggtgtagtta atacctgccc caacataagg acggaatttg ctgccggcat caccaaaata
     1141 ccactgcgcc atcagtgttg gcggcagatg atgaacggtt gcaatatcgc cggtcgcccg
     1201 ggtgccgatt ttatggcgga acggcgtcgc tgccagtaat tccacaccaa tgttgtcggt
     1261 cgccatataa gtaaacgtca ggcccagttg cgtgttattg gtcacgctga atccacccag
     1321 acttcctaac gtaccaccag caccttctgt tggacgtacg gttgcagaac ctgcacgcat
     1381 aaaaaattcg cctgcttcat gcgcaaaggc actgccagag agaagagttg ttactgccaa
     1441 agccgccact gttaactttt tcatatccgc tccgtcgtta tggttataaa aacgtgacga
     1501 atatacctac attggagtaa caaatgatcc tacatagatc acattacata tgataattca
     1561 ccctttattg atctggatta atttagaagt tgcattgaga atcctggtta acgtgattta
     1621 aatcaatttt tagatatttt cgctacgcaa atattaactc gttcgccact tgccgcggtt
     1681 tgcccacgcc tgaaggccag caattacctc cgctttacaa agatatgctt ttccatacaa
     1741 gacctgatgc tgccacccga ggcttaacca gggtacaatt gcccgctaat taacacctgc
     1801 ataaaactca aggagagtgc atgtctatca cggcgcagtc cgtataccgt gacaccggaa
     1861 atttcttccg taatcaattt atgaccattc tgttggtatc gttgctatgt gcgtttatca
     1921 cagtggtgtt agggcatgtt ttctcaccca gtgatgcaca gcttgcgcag ctcaatgacg
     1981 gcgtgcccgt tagcggcagt agtgggttgt tcgacctggt tcagaatatg tcaccggaac
     2041 aacaacaaat tttgctgcag gcttcagcgg cgtccacttt ttcaggatta atcggtaacg
     2101 ccattctcgc cggaggcgta atattaatta tccagctggt gtctgcgggt cagagagtca
     2161 gtgcgctgcg agctattggt gccagtgcgc cgatattgcc caagttattt attctgattt
     2221 ttctgactac ccttttagta cagattggca tcatgctggt ggtcgttccg ggaattatca
     2281 tggccatttt actggccctg gcaccggtga tgctggtaca ggacaaaatg ggcatttttg
     2341 cctcgatgcg tagcagtatg cggctgactt gggcgaatat gcgtctggtg gcacccgcag
     2401 tactgagctg gttgctggca aaaacactgt tgctgctttt tgcctcttct tttgccgcat
     2461 taaccccgga aattggtgcc gtactggcga acaccttgag caacctgatt tcagccatat
     2521 tgctcatcta tctgttccgc ctgtatatgt tgattcgcca ataaccttaa gcatttgatt
     2581 acggaatcgt aaaatgaagc agtttcttga ttttttaccg ctggttgtct ttttcgcgtt
     2641 ttacaagatt tatgacatct atgcggctac tgcggcgctg atcgtcgcca cggcgattgt
     2701 gcttatctat agctgggttc gctttcgtaa ggttgagaag atggccctga tcacttttgt
     2761 tctggtggtc gtcttcggtg gcttgacgct gttcttccac aatgatgagt ttattaaatg
     2821 gaaggttaca gtcatttatg ctctgtttgc gggagccctg ttagtcagcc aatgggtgat
     2881 gaaaaagccg ctaattcagc ggatgctggg taaagaactc acgctgccgc aaccggtatg
     2941 gtcgaagcta aatctggcct gggctgtttt ctttatcctt tgcggtctgg caaacatcta
     3001 catcgcattc tggctgccgc aaaatatttg ggtcaacttt aaagtctttg gcctgaccgc
     3061 ccttacctta atcttcacat tgttaagcgg tatctatatc taccgccaca tgccgcagga
     3121 agataaatcc taaccagact gccagtcctt cggggctggc gctgtatctc ctctgttctc
     3181 ctcaggaaaa tcatagtagc atcgcgcctg tgattttcct tttaagtcgg ttttaccatg
     3241 tctacaacac ataacgtccc tcagggcgat cttgttttac gtactttagc catgcccgcc
     3301 gataccaatg ccaatggtga catctttggt ggttggttaa tgtcacaaat ggatattggc
     3361 ggcgctattc tggcaaaaga aattgcccac ggtcgcgtag tgactgtgcg ggttgaagga
     3421 atgactttct tacggccggt tgcggtcggc gatgtggtgt gctgctatgc acgctgtgtc
     3481 cagaaaggga cgacatcggt cagcattaat attgaagtgt gggtgaaaaa agtagcgtct
     3541 gaaccaatcg ggcaacgcta taaagcgaca gaagcattat ttaagtatgt cgcggttgat
     3601 cctgaaggaa aacctcgcgc cttacctgtt gagtaatagt caaaagcctc cggtcggagg
     3661 cttttgactt tctacttact gaatttcggt ggtgccgtta attttaaaca ggatattcac
     3721 cacaatccca ctgcctggct tacccggctc ataacgccat ctgcgcatcg catttttcac
     3781 ctcacgctca aacatgttcg caggcttggc tgagaggatt tgtacgttat ccacgcgacc
     3841 atccggcgtg acgtcaaatt taactttaac ctgcccttca atgcgcaatg cctgtgctcg
     3901 tgccggatac tgcggctgat tacggcttaa tgcgcgtggt cctgaagcca cactggtaac
     3961 cggcttgctg gttgcagccg ttgccgtact tgatgtgggg cgcgcgggtg ccgtattttc
     4021 aaacggtgat gccggacgcg actctacggg tttgacatcg cgttttggct gctcctgtac
     4081 ctttttcacc ggcttcggtt ttggcttagg tttcggcttc ggcttttcaa tgaccaccgg
     4141 tgcttctttt ggcggttcgg ggatcggctc aggttccggt tctggctcta ccaccggctc
     4201 cggtggcggc tgaacggctt gtggcggttc gagatcagca ggcgcaacca tcgtgacaga
     4261 aattggctgc gcaggcgcag gtagttcaat aacctgatgt accgaggtat agagcagacc
     4321 cgccacaaca gcaccatgaa tgcagaccga aagtaacgtc ggccagggga agcggcgagg
     4381 taaatcaagg gtcattgtat tgtagctatt cggcattgta gcattagcca taacgtttat
     4441 aagttcatct ttaaatgata atcaattgca tgggagttta tcaccgtgag aaccgtaata
     4501 acatacttga gattctcatc agccatacaa ggcgctgaag gtgcagattc aaccagacga
     4561 caaaacgacc tgttcaagca gtggctgaag aagaatggcg atgctcaaat agtagcgtct
     4621 ttcagtgatg aagggttgag tggttacaaa ggtaaacacc ttactggtca gtttggtgac
     4681 atgttagccc gtattgaggc tggggagttt ccagaaggta caattctgct agttgagagt
     4741 atcgaccgta taggccgatt agagcatctt gaaacagaag ccttgatgaa ccgcatctta
     4801 ggcaacggga tagagattca cacattgcaa gatggcctta tctacacaaa ggatgcttta
     4861 gccgatgacc ttggcataag catcatccag cgtgtcaaag cctacatagc gcatcagaaa
     4921 tctaagcaga agtctttccg tgtttctcag aaatgggggc aacgtgcaaa acttgccctt
     4981 gctggtgaac aacgtcttac aaagatggtt cctggatgga ttgaccctga gacttttaaa
     5041 ctcaatgaac acgctgagac tgtgagattg atattcaaac tgctgctaga tggtgaaagt
     5101 ctgcataaca ttgcacgtca ccttcaaagc aacggtataa agtcgtttag tcgccgtaaa
     5161 gatgctaatg ggttctctgt tcactctgta cgcaccattc taaggtcaga gacaacaata
     5221 ggcacgttac cagcatcaca acgtaatgac cgccccgcta taccgaacta ctacgaaggt
     5281 gttgtagata taccaacgtt caataaagct caagagattc tcgacaagaa tcgtaaaggc
     5341 cgtacacctg caagtgacaa cccactaacg attaacatct tcaaaggtct gtttaggtgt
     5401 cagtgtgggg ctagtgtcca tcctaccgga acaaagaata agtatgctgg ggtctacagg
     5461 tgcaataacc acttagacgg tcgctgtgat gttccaccgt tgaagcgtaa accgtttgac
     5521 cgatggatga ttgataattt tctggggatg attgacgtgg ggaatgatgg agaatcagag
     5581 agaaagattg cagcgttaca gcatgaggtt gaaattgtca cagccagaat caagaaagct
     5641 accgccctac ttcttgagat ggatgatatt gatgaactaa aaattcagct taaggaactg
     5701 aaccagaagc gcacagaact acagaccacg attgataaca tgaggcgtaa ggcttcactc
     5761 actgataagg aactacccca gcttaaagat attgacctta tgactaaagc gggtcgtgtc
     5821 gagtgtcagt tgattttgtc caagcatcta aaagggctta cattgggtaa ggattcggta
     5881 actgtaacgc tacagaacga cactgaaatc accataccta caaacccgtt accgctaaat
     5941 gatgggtcac caatctttga aattgctgat aaagagctac tggatattga cgcttatcaa
     6001 ctgtaatttt caggggctat gaagcccctt tttatgtctc aataagtgcc cttcttgtta
     6061 gggttcacag agtactttcg cagagcatta cccggcttaa gcacctttga agcttgctca
     6121 ttagcggcat ctgagaaggt gctctcacca tttttaacgc taacgctctt agagcacgtt
     6181 acacgggctt ttagcgcttc atctgataac tcacttgccg aatagccaaa aagctcttta
     6241 caggtcagca tagggcttcc aaggctcgtt gtagtgtttc cggtgttctt tttactggta
     6301 gcaccgccat cattcgtggt agcggtcttt ccagtagcag ccgaaacatc agtctgacct
     6361 acgattgaac gaaactctac accttggaag gtcatctgaa tacgaagacc gttagcaatg
     6421 gtatgctctt tactcgcttt aaactgagta atgatactat cagtgatagc tattccatct
     6481 ctacaaacaa tagtgatgat ttgcttctgg tctcgccatt gttcaaccgt agaaataaag
     6541 gtttctacaa gttgcccctg tctggttaat agaactgaac ccgtcgaatc aaccactaca
     6601 acaccgctaa tagagatggt cttaggtgct ctct
//
LOCUS       DAZPBM010000202         6553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPBM010000202 DAZPBM010000000
VERSION     DAZPBM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.203"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUZQ_003783"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHUZQ_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1027377.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUZQ_003784"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHUZQ_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1027378.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHUZQ_003785"
     CDS             2408..3157
                     /locus_tag="ACHUZQ_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1027379.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MGLQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3745
                     /gene="yieF"
                     /locus_tag="ACHUZQ_003786"
                     /gene_synonym="chrR"
     CDS             3179..3745
                     /gene="yieF"
                     /locus_tag="ACHUZQ_003786"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1027380.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHUZQ_003787"
     CDS             complement(3799..5136)
                     /gene="adeP"
                     /locus_tag="ACHUZQ_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1027381.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5302..5967
                     /gene="yieH"
                     /locus_tag="ACHUZQ_003788"
     CDS             5302..5967
                     /gene="yieH"
                     /locus_tag="ACHUZQ_003788"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1027382.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6034..6507
                     /locus_tag="ACHUZQ_003789"
     CDS             6034..6507
                     /locus_tag="ACHUZQ_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR1027383.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1608 a         1655 c         1659 g         1631 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggctgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagtatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatgggac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
     3481 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
     3541 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
     3601 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc ggcaaaccgg
     3661 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
     3721 gtttattcag cgagttaaga tctaaataaa aaacccgcca gtaatcatgc atggcgggtt
     3781 tttaatgcgc tatcgatttt aatgagcgtc gataaataca atcttcagga taaacagcag
     3841 cgcaacgatg attacgcacg ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac
     3901 gcagtaggag ataaagccca gcgcaatacc ttcggtaatc gaaaagctga acggcatcat
     3961 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgagcaag
     4021 acttgaggtc atcaacacgc caacgtagat caacgcgcca gctgcagcgt agcctggcac
     4081 cattcccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
     4141 cgtcagaccg gtacgaccgc caaccgatac gccggaagag gactcaatat aagccgtaac
     4201 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
     4261 cttcatgcgc gggaatttcc ccttctcatc cgccagacct gctttatcgg tcacgccaat
     4321 cagcgtaccg gaggagtcaa acaagttgac caacatgaaa gagaaaatca cccctgccag
     4381 cccgaggtta aacgacccgg ctaaatctac atgacccaca actgtcatta cgctcggcgg
     4441 cgcagaaacg atgccattgt agtgcacatc acccagcatc cagcccagca gcgtcgtcac
     4501 cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag gccagaatgg cgatgatgaa
     4561 gaaaccgagg atccccagca gcacgctgtg agaagtcaga ttaccgatgc tcaccagcgt
     4621 ttccgggtta gcgacaatca cacctgcgtt tttcagcccc atcatgccaa tgaacagacc
     4681 gataccgctg gtaataccca cacgcagact taccggaatg ttagcaatca tccagtaacg
     4741 aacgcggaaa atggtcaaaa gaagcagtcc gacagcgccc cagaagattg cgcccatacc
     4801 gatctgccac ggcagcccca ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc
     4861 catcgcagga gccagcgcca ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa
     4921 agcagcaatc agacaggttg ttacgaagac ggcactggtg tccatgccag caacgcccag
     4981 aatttgtggg ttaacaaata cgatgtagac catcgtcagg aaggtggtga acccggcaat
     5041 cacttcagtt cgtgccgtag taccatgttc gcgcagttta aaaactcgtt ccagcatccc
     5101 ctgaccagaa gtctgggtgg tatgttgatg actcattatc tatttccgaa caaggaggga
     5161 aaatccgtcg ctatcgtata ccaaaatgcg acaataggcg cgtttgtgag agactttttt
     5221 tattggattt acttatacgg caacgattgc gttgcgcaaa tcggctctac gaaaacgtta
     5281 aactgattaa aaaggaaagg catgtcccgg atagaagcgg tatttttcga ctgcgacggt
     5341 acgctggtcg acagtgaagt catttgctcc cgcgcatatg taacgatgtt tcaggaattt
     5401 ggtattacgc tcgatcctga agaggtattc aaacgtttca aaggtgtaaa actgtacgaa
     5461 attatcgata ttgtttccct tgaacatggt gttacgttag cgaaaacaga atctgaacac
     5521 gtttaccgtg cagaagtcgc tcggctgttc gattcagaac tggaagccat cgaaggggct
     5581 gaagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg tatctaacgg cccaaataac
     5641 aaaatgcagc attctatggg caagctgaat atgttgcact acttcccgga taaactgttc
     5701 agcggctacg atattcagcg ctggaagcca gacccggcgt taatgttcca tgcggcaaaa
     5761 gcgatgaatg taaatgtaga aaactgcatt ctggttgatg actcagttgc cggtgcgcaa
     5821 tctggtatcg acgcaggtat ggaagtgttc tacttctgcg ccgacccgca caataagccg
     5881 atcgttcacc cgaaagtcac cacctttacc catctttcgc agttaccgga actgtggaaa
     5941 gcgcgtggtt gggatattac ggcatagttc ttcacactcc cttcacttac cccgcttaaa
     6001 ttggcgctca aaggtaagta aagggagttt gatatgtctg tttcacgtcg ggtaatacat
     6061 cacggacttt attttgcggt tttaggaccg ttaattggtg ttctgtttct tgtcctctac
     6121 atattcttcg caaaagaacc gctggttctt ttggtaataa tacaggtact tccattgttt
     6181 ctcttattgt cgattactac gggagctatt cctgcattgt taaccggcgt gatggtcgcc
     6241 tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt gtctggctgg tggcataggt
     6301 ggcgtcgtta tcaccgagat ctattgtgca gttattgtac atattaaggg catggcttcc
     6361 tcggagttgt ttgaaaacat tctttctggt gacagtctcg ttgtccgcat cattcctgca
     6421 ttgctggcag gtgtggtgat gagcagaatc attacccgtc tgcccggatt ggatatttca
     6481 tgtcctgaaa cagactcttt aagttaagcg ggatacttta tctttgggct actcaaaagc
     6541 agacaggatg tta
//
LOCUS       DAZPBM010000203         6499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPBM010000203 DAZPBM010000000
VERSION     DAZPBM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.204"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUZQ_003790"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUZQ_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHUZQ_003791"
     CDS             complement(1751..2509)
                     /locus_tag="ACHUZQ_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1027384.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUZQ_003792"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUZQ_003792"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1027385.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUZQ_003793"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUZQ_003793"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1027386.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUZQ_003794"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUZQ_003794"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1027387.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHUZQ_003795"
     CDS             complement(5474..6079)
                     /locus_tag="ACHUZQ_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027388.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6499)
                     /locus_tag="ACHUZQ_003796"
     CDS             complement(6082..>6499)
                     /locus_tag="ACHUZQ_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027389.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1638 a         1695 c         1663 g         1503 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgt
//
LOCUS       DAZPBM010000204         6492 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZPBM010000204 DAZPBM010000000
VERSION     DAZPBM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.205"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(113..487)
                     /locus_tag="ACHUZQ_003797"
     CDS             complement(113..487)
                     /locus_tag="ACHUZQ_003797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027390.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            complement(810..2744)
                     /locus_tag="ACHUZQ_003798"
     CDS             complement(810..2744)
                     /locus_tag="ACHUZQ_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1027391.1"
                     /translation="MLRPICYERVSSIQQVDMGKGLDDQRSSLTAYLDKNADKFTDER
                     IFITDAGLSAFKNANISPESQLGIFLQDVGNRKYGVGDALVVLSLDRLSRRSSWAEST
                     IQYIVNSGIEIHDISTNLVLRVDDPMSKIHMELIQHRSHNESLMKSVRAKLAWDNKIT
                     RAVQNGEVISNRMPNWLRNVDNKYQVIPEQAELIVKCFMWYREGLSTGEIVKRIGRKW
                     QMVTVSRLIRDRRLLGEHERYNGEVVPNVYPKIIDEELFLTANRMMDRVMLEKKKPAE
                     DLLLEPEVVREIFKLYETGLGSGAIVKRLPKGWSTVNVLRVLRDKKVVTMKIIDNLTF
                     ERANEKLSRNGIANRIRKDITIAQDDYITNLFPKMLKCGHCGGNIAIHYNHVRTKYVI
                     CRNREEKKICDAKSIQYMRIEKNILQLVKNVDFNKLMMEGKSDSDSLLASLREELSTL
                     QKEAETYQVKINERKAAGKKASLPLMTGLTEVQDRIEEIESEILNSKNIKEIPVFDFD
                     INKVLDPLNVELRAKLRKEVRLVLKSVKYWVFEKRIFVQLEYFNDVLSHTLVIDNKRG
                     GGEVLHEIVISETSGERVYTVNEEGKTVFIASVTSGTDMWSLAISRTKILDDVGLYLN
                     LLKREKFEIIVNEDMIDWMD"
     gene            2824..4758
                     /gene="yeaG"
                     /locus_tag="ACHUZQ_003799"
     CDS             2824..4758
                     /gene="yeaG"
                     /locus_tag="ACHUZQ_003799"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR1027392.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            4871..6154
                     /locus_tag="ACHUZQ_003800"
     CDS             4871..6154
                     /locus_tag="ACHUZQ_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR1027393.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6301..>6492
                     /locus_tag="ACHUZQ_003801"
     CDS             6301..>6492
                     /locus_tag="ACHUZQ_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1027394.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLL"
BASE COUNT         1814 a         1516 c         1342 g         1820 t
ORIGIN      
        1 tactatctcg taaagtaatt tagtaaacca ttccattttg aagtaccccc attcacattc
       61 aactttatag tatttagtga gggagtactt aaaacgtaac aatttggatg tgttagctaa
      121 taagttttag tataggtttc gaataaatga caagtccagt aactgaacat tttgcttctt
      181 ttatctcttc aattagttcc attgatattg gatgtttgaa agcttgagta cactcctgac
      241 agcctgcctc cgttagcgga aactcatctg ttataacttg aatttcagaa gaatacggca
      301 aaaaaatgta ctgaacttta attttgttgg aaaattcgta cttgcataag ctctcatctt
      361 gagaatcatt ccgctttgaa aaaagcctta attgattctc ttcattcaat gagatcccaa
      421 tatcataatt ctcagggaaa aattgacgga atttttcttt ttttgcttta ttttctttct
      481 tacgcatttt ggcaatcctc tctttagaat tcatctcatg gtttcatcgc tcctattcgt
      541 tagtgaatga cgctatggta ctggtttaac gttgaaaagt caaaaaagtt aattttaccc
      601 ttgactattc taacacacgc tgatataaat gatttgctgt attttgcacg gaaatcttaa
      661 acccatcggg tgcgtagtta tatccaaaat tcataatccc tcttttattg ctctatctac
      721 aatgccgtta gatttcgaca tcgtagatat attgttcaaa tattaactcg cataattcat
      781 tctacaagac cataaattct cattagcttt taatccatcc aatcaatcat atcttcatta
      841 acaatgatct caaatttttc acgcttgaga agattaagat acaatccaac atcatcaagt
      901 attttggtac ggctaatggc aagcgaccac atatcagtac cagatgtaac ggaggcaatg
      961 aaaacggttt tgccctcttc attgacagtg tagacacgct cgccgcttgt ttcagagatt
     1021 acgatctcat gtagtacttc acccccgccc cgcttgttat caatcacaag cgtatgggat
     1081 aggacatcat tgaagtattc caactgtaca aatatacgct tttcaaaaac ccagtactta
     1141 acagatttca gtactaatct tacttctttc cgtaactttg cacgtaactc gacattcaat
     1201 ggatcaagta ctttattaat atcgaaatca aatacaggga tttctttgat gttctttgaa
     1261 tttaatatct cagattctat ttcttcaatt ctatcctgta cctcagttaa acccgtcatt
     1321 aatggtaacg aggctttctt acctgctgcc ttacgttcat taattttaac ctgataggtt
     1381 tctgcctctt tctgtaaagt acttaactct tctcgcaatg atgcaagcaa tgaatctgaa
     1441 tccgacttac cttccatcat caatttattg aagtctacat ttttgacaag ctgtagaatg
     1501 ttcttttcaa ttctcatgta ctggatagat ttagcatcgc agatcttctt ttcttccctg
     1561 ttacgacaaa taacatactt agttcgtaca tgattatagt gaattgcaat attgccccca
     1621 cagtgaccgc atttcagcat cttcgggaag aggttagtaa tgtaatcatc ctgagcaatg
     1681 gtaatgtcct tacggatacg attagctata ccattccgcg ataacttctc attagcccgt
     1741 tcaaaggtga ggttatcgat aatcttcatg gtaactactt tcttatcacg tagtaccctc
     1801 agtacattga cggtactcca ccctttcggc aagcgtttta caatcgcccc tgaaccaagg
     1861 cctgtctcat aaagtttaaa aatctctcgg acaacttctg gttcaagtaa tagatcctct
     1921 gctggcttct tcttctccaa cataacgcga tccatcattc tattcgctgt caggaacaac
     1981 tcttcgtcga tgattttcgg gtaaacgttt ggtactacct caccgttgta acgttcatgc
     2041 tcgcccaaca aacggcgatc acgtatcagc ctggacacag tgaccatctg ccactttcta
     2101 ccgatacgtt taacaatctc gccagtactc aacccttccc tataccacat gaaacattta
     2161 acaatcagtt ctgcctgttc cggtataact tggtacttgt tatctacatt tctcaaccaa
     2221 ttaggcatac ggttggagat tacttccccg ttctgtactg cacgtgtgat tttgttatcc
     2281 catgccaatt ttgcacgtac cgatttcatc aatgactcat tgtgtgaacg gtgctgtatc
     2341 agttccatgt gaatttttga cataggatca tcaacccgta acacaaggtt tgtagagata
     2401 tcgtgaatct caatcccact attcacgatg tactggatag tactttcggc ccagcttgat
     2461 cgacgcgata gacgatccag cgataaaacc acaagtgcgt cacctacgcc gtacttgcgg
     2521 ttgcccacat cttgcaaaaa tatcccaagc tgagactctg gagagatatt ggcatttttg
     2581 aatgcggaaa gccccgcatc agtaatgaaa atcctttcgt cagtgaactt atcagcattt
     2641 ttatctaagt atgcagtcaa tgatgatcgt tggtcgtcca aacctttacc catatcaact
     2701 tgctgaatgc ttgatactcg ctcataacaa ataggacgga gcatattttt tatcccgttg
     2761 aagaattacg ctaaaagtgc tgattcacaa atgttcccag agtatacaaa aggacggcat
     2821 accatgaata tattcgatca ctatcgccag cgatatgaag ctgccaagga cgaagagttc
     2881 acactgcagg agtttcttac cacttgtcgg caagatcgca gtgcttatgc caacgcggct
     2941 gagcggctat tgatggctat cggtgagcct gtcatggtcg atacagccca ggaacccaga
     3001 ctttctcgac tcttttctaa ccgggtcatt gcacgttatc cggcatttga agagttttac
     3061 ggcatggaag acgcgattga acagattgtc tcttatctga aacacgcggc tcaggggctg
     3121 gaagagaaga aacaaatcct gtatctgctg gggcctgtgg gtgggggtaa atcatcgctt
     3181 gctgagcgac tgaaatcatt aatgcagctc gtaccgattt atgtattgag cgccaacggt
     3241 gagcgtagcc cggtcaacga tcatccgttc tgtcttttca atccgcagga agatgcgcag
     3301 attctggaaa aagagtatgg cattcctcgc cgctatctcg gcaccatcat gtcgccgtgg
     3361 gcggcaaaac gcctgcatga atttggtggc gatatcacta agttccgggt agtgaaggtc
     3421 tggccgtcaa ttctgcaaca aattgctatc gccaaaacag aacccggtga tgagaacaac
     3481 caggacatct ccgcgctggt agggaaagtc gatattcgta aactcgaaca ctacgcgcag
     3541 aatgacccgg acgcctacgg ctattccggt gcgctgtgcc gcgccaatca ggggatcatg
     3601 gaatttgtcg agatgtttaa agcaccgatt aaagtgctgc atccgctgtt aaccgctact
     3661 caggaaggta actacaacgg gacggaaggt atctccgccc tgccgttcaa cgggattatt
     3721 ctcgcccact cgaacgaatc cgaatgggtc actttccgta ataacaaaaa caacgaagcc
     3781 ttcctcgacc gtgtttacat cgtgaaggtg ccgtattgct tacgcatttc cgaagagatc
     3841 aaaatctacg agaaattgct taatcacagt gaattgactc acgccccatg cgcccctggc
     3901 acgctggaaa cactgtcacg tttttccatt ctttcgcgcc tgaaagagcc agaaaactcc
     3961 agcatttatt caaagatgcg ggtttatgat ggcgaaagtc tgaaagacac cgatcccaaa
     4021 gccaagtcgt atcaggaata tcgtgactac gccggtgtcg atgaagggat gaaaggtctg
     4081 tcgacgcgtt ttgcgtttaa gatcctctcc cgcgtgttca acttcgatca tgtagaagtg
     4141 gcggcaaacc cggtccatct gttctacgtc ctagaacagc agatcgagcg cgagcagttc
     4201 ccgcaagagc aggcagaacg ctatctggag ttcctgaaag gttatctgat cccgaaatat
     4261 gccgagttta tcggcaaaga gatccagacg gcctaccttg aatcctattc cgaatatggg
     4321 caaaacattt tcgaccgtta tgttacctac gcggatttct ggattcagga tcaggagtat
     4381 cgcgatccgg ataccgggca gctgtttgac cgcgagtctc ttaacgccga gctggagaaa
     4441 atcgagaaac cggcggggat cagtaatcca aaagatttcc gcaacgagat tgttaacttc
     4501 gtactgcgcg ccagagcgaa taacagcgga cgcaatccga actggaccag ctatgaaaaa
     4561 ctgcgcacgg ttatcgagaa gaaaatgttc tccaataccg aggagctgtt gccggttatc
     4621 tcgtttaacg ccaaaacgtc aaccgacgag cagaagaaac acgacgactt tgtcgaccgt
     4681 atgatggaaa aaggctacac ccgtaaacag gtgcgtttac tgtgcgaatg gtatttgcgc
     4741 gtacgtaaat cgtcttaaca accctggccc ggtacacatg ctaccgggcc tataacgaca
     4801 gcgaaccgtg ggccagagaa atggcaacac aggcgtagca tacagttggc aaatgtagta
     4861 cggggggcat atgaccgggt ttattgaccg gcgtctgaac ggcaaaaaca aaagcatggt
     4921 gaatcgccag cgttttttac gccgttataa agcgcaaatt aaacagtcga tctccgaggc
     4981 cattaataag cgttcggtga ctgacgtcga cagcggcgaa tccgtatcca ttcccacgga
     5041 agatattagc gaaccgatgt ttcatcaggg gcgtggcggc ctgcgccacc gcgtacatcc
     5101 gggcaatgac catttcgtcc agaacgaccg aattgaacgt ccccagggtg gcggcggagg
     5161 ttccggcagt ggtcagggcc aggccagcca ggatggtgaa ggtcaggatg aatttgtctt
     5221 tcagatttcg aaagatgagt atcttgatct gctctttgaa gatttggcct taccgaatct
     5281 gaaacaaaac caacaacgcc agctgaccga atataaaacg catcgggcgg gttataccgc
     5341 taacggcgtt ccagccaata tcagcgttgt gcgttccttg cagaactcac tggcgcgacg
     5401 cacagccatg acggcgggca agcgacggga acttcatgcg ctggaagaga atttggccat
     5461 catcagcaac agtgaacctg cgcaactgct ggaagaggaa cgtctgcgca aagaaattgc
     5521 agaattacgt gccaaaattg aacgcgtccc ttttattgac accttcgatt tacgttacaa
     5581 gaactacgag aagcagcccg atccctccag ccaggcagtg atgttttgcc tgatggacgt
     5641 ttccggttca atggatcaat ccactaaaga tatggctaag cgtttttata ttctgctgta
     5701 tctgttcctc agcagaacgt ataagaacgt ggaagtcgta tacatccgcc atcataccca
     5761 ggcgaaagaa gtcgatgaac atgagttttt ctactcgcag gaaacaggcg gcaccattgt
     5821 ttccagcgcc ctgaaactga tggatgaggt agtgaaagag cgttataacc cggcacagtg
     5881 gaatatttac gctgcacaag catcggacgg cgataactgg gccgatgact ctccgctttg
     5941 ccatgaaatc ctggcgaaaa aattattacc tgttgttcgt tattacagct atatcgaaat
     6001 tacccgtcgt gcacatcaga cattgtggcg agaatatgag catctgcaat ctactttcga
     6061 caactttgcg atgcagcaca tccgcgacca ggatgatatt tatccggtgt tccgtgaact
     6121 gtttcataaa caaaatgcaa cagctaaaga ctaaaactat cagccaggtc attatcgcct
     6181 ggctgatttt tagcttactg taaattatct catttattac atacattagc ttacaatcgc
     6241 tttaaatatg acagcataac ctttacataa tttagttcca gaaaacaatc attcggaaaa
     6301 atgattcagt caacacgtat ttccatgggg ttattcttta aatatttttt atcgttaacg
     6361 aaaattgatc ctggtcaaaa ctatatatct ctgccatcaa taaaatccag cactcacatt
     6421 gctctccttt ttatggtttc tatgggtaca caaaaattaa aagctcaaag cttttttatt
     6481 ttcagtttat tg
//
LOCUS       DAZPBM010000205         6361 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPBM010000205 DAZPBM010000000
VERSION     DAZPBM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.206"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHUZQ_003802"
     CDS             <1..331
                     /locus_tag="ACHUZQ_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1027395.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUZQ_003803"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHUZQ_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1027396.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUZQ_003804"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHUZQ_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1027397.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUZQ_003805"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHUZQ_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1027398.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUZQ_003806"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHUZQ_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1027399.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHUZQ_003807"
     CDS             complement(4567..5001)
                     /gene="uspF"
                     /locus_tag="ACHUZQ_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1027400.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHUZQ_003808"
     CDS             complement(5142..6275)
                     /gene="ompN"
                     /locus_tag="ACHUZQ_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR1027401.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT         1723 a         1506 c         1544 g         1588 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaact
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt atcgtagctt
     4261 attgttgtcg ctgataatgt ttaacatatt gaaaaataaa taaaatactt atgggtcaga
     4321 tagttgtgat ttttgccctt acttgttcag gttgtattgt tctttcttac taatttcttg
     4381 attttgcgac atttaaaagc gactcaattc gttatatggc atcagaagag tatgcgtcat
     4441 gccggaacgc ccagcataag aaatctgata taaaactgtg gcgtgtatgg tacggattag
     4501 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     4561 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     4621 ggaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     4681 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttcct caacatggac
     4741 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     4801 ttccgctttc aggtcatcca ttgccggtaa ttctgcggaa tacgctaaac ccagagaggc
     4861 atagtaaggc agtgaaggta ttaccgtcag gaaatgaacc tctgcatcgt caatctttgc
     4921 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     4981 gacaagaatc gttctgttca taaaacctcc tgttttagta tccgcataaa gtgtaacgcc
     5041 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     5101 aataagcaga aaaagcccgc cataacagcg ggcaggagga tttagaactg ataaaccaga
     5161 cctaaagcga caatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     5221 tccaacaggt tgattttata gtcaacataa gtggacatgt ttttattgaa atagtaagtc
     5281 gcgccaacat cggcgtattt aacaagatct ttatcatcaa cacctgccgg gttgtctgca
     5341 ccatcggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     5401 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     5461 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     5521 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacccgcc
     5581 gcagtatggt taacctggtc attggtgcgg tcagatgagg tgtatgccgc accagcgcta
     5641 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccatt ttcatggcga
     5701 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     5761 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     5821 gcgacgccgt tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     5881 aattcaggca gcatatcggt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     5941 taatcgaaag aaccgtaatc tgcaaatttc agcccggcaa atgccagacg ggtccatgac
     6001 tggttttttg aagattcagt gttgtttgcc tgaatattgt attcccattg accgtagcca
     6061 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgcgcata gctctgatcg
     6121 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     6181 aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgc ggcgagcagg
     6241 gcaggaatta aaagtgccag tactttgctt ttcattgaat aaatccttta gttattttat
     6301 ttgcctttta tccccattcc ggggagtgaa aacatcctaa aggagaagtt cagttagccg
     6361 a
//
LOCUS       DAZPBM010000206         6347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPBM010000206 DAZPBM010000000
VERSION     DAZPBM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.207"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..364
                     /gene="ydjO"
                     /locus_tag="ACHUZQ_003809"
                     /pseudo
     CDS             1..364
                     /gene="ydjO"
                     /locus_tag="ACHUZQ_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(368..1759)
                     /gene="tcyP"
                     /locus_tag="ACHUZQ_003810"
     CDS             complement(368..1759)
                     /gene="tcyP"
                     /locus_tag="ACHUZQ_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR1027402.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1892..2482)
                     /locus_tag="ACHUZQ_003811"
     CDS             complement(1892..2482)
                     /locus_tag="ACHUZQ_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1027403.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2645..3313)
                     /gene="hxpB"
                     /locus_tag="ACHUZQ_003812"
     CDS             complement(2645..3313)
                     /gene="hxpB"
                     /locus_tag="ACHUZQ_003812"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1027404.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3460..3996
                     /locus_tag="ACHUZQ_003813"
     CDS             3460..3996
                     /locus_tag="ACHUZQ_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1027405.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4037..4897)
                     /locus_tag="ACHUZQ_003814"
     CDS             complement(4037..4897)
                     /locus_tag="ACHUZQ_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1027406.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5003..5293)
                     /gene="ghoS"
                     /locus_tag="ACHUZQ_003815"
     CDS             complement(5003..5293)
                     /gene="ghoS"
                     /locus_tag="ACHUZQ_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1027407.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5394..6323)
                     /gene="pfkB"
                     /locus_tag="ACHUZQ_003816"
     CDS             complement(5394..6323)
                     /gene="pfkB"
                     /locus_tag="ACHUZQ_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1027408.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1641 a         1591 c         1551 g         1564 t
ORIGIN      
        1 atacatcctg ccgggagcat ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt
       61 tccttcgata atatcaatgc ttaatatcaa tcttcttttt aatccactaa atcttccggg
      121 aatgggaagt ggtgtgcttg aagatattat gagtattcca gatagttcac ttagaaaacg
      181 tctcgggtat gaggttttaa gcttctcact acaggcacat tcattgagcc aggaatgtat
      241 tgataagttg gacatttttt tcgctgacga tctgtttaaa tatgagtcag tatgcatcgc
      301 cgcgatggaa catttaaaaa gtaaagcaac ggcccctata caaaacggac cgttgccagc
      361 ataagaatta acggtgtgcc agttcggcgt cgtcttcgct atccagaatg gctttatcgg
      421 tttgcttcag ccactggctg gtcagcgtgc cagctgtcat cgagccacta acgtttagcg
      481 ccgtacggcc catgtcgata agcggttcaa cggagattaa cagcgccacc agggttactg
      541 gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca ccgacaccgg
      601 caacgcctgc ggaactaacg gtaacaatac cgaccagcgt cgcaatccac atcgggtcca
      661 gcgggttaat gccaaccgta ggcgcaacca tcaccgccag cattgccgga tacaaaccgg
      721 cgcagccgtt ctgaccaatg gttgcaccga aagaggcggc gaaactggcg atggattcag
      781 gaacgcccag acgacgcgtt tgtgcttcca cattcagtgg gatagacgca gcactggaac
      841 ggctggtaaa ggcaaacgtc agcacaggcc ataccttacg gaagtacttc agcggactca
      901 cgccattaat gcccagcaga atgccatgca ctgcaaacat aatcagcaga ccgaggtagg
      961 acgcgacaac gaaacttccc agtttgatga tgtcttgcaa gttagaacct gcaaccactt
     1021 tggtcattag agccagaacg ccgtaagggg tcaactgcat gacaaggcga accagtttca
     1081 tcacccagct ttgtagggta tcgatagcgg ctaagacgcg ttcacctttc ggcgcatcat
     1141 ccttcagcag tttcaatgca gctacgccga ggaatgcggc aaaaattacc acgctgataa
     1201 ttgacgtcgg attggctccg gtaagatcgg caaacgggtt tttcgggata aaggacaaga
     1261 ccagctgcgg aacgctcaga tcagagactt taccaacata gttactttca atggcgttca
     1321 gacgtgcagt ttctgcacca ccctgaacca gaccttcagc cgtcaaacca aacaggttgg
     1381 tgaccagcac accgaccagc gccgcaatca gcgtggtaaa caaaagcgta ccgatggtca
     1441 gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag
     1501 aggcgaagac taacggcata acgatcattt gcagcagttg aacatagccg ttaccaacga
     1561 tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca
     1621 gggcaaggcc aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc
     1681 actgtttatg gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg
     1741 caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca
     1801 gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat
     1861 tgcttattcc agaaaggtga tttttgcctt tttagtattc aacctgatgc ttaataagcc
     1921 gatgaaactg gatctgcaag gtattgatca tttgcgatga ccaacgaaca gcgctgttct
     1981 cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt
     2041 ccagttggtt gcccttttgg ggaaccagga taggcaagcg gaaacgccag cggcatggcc
     2101 agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc aaataaccca
     2161 gcaccattcc ttgtagcgca tcagccggaa tgaaccagcc ttccggaacc ttaaggtaaa
     2221 aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag
     2281 cgcgggcgat cggttttgat atccatttta atcgctgccc aagaaacgac tttgggtgat
     2341 cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct
     2401 gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga
     2461 gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg
     2521 atttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta
     2581 atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc actgcccctg
     2641 gtcattaacc gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg
     2701 ctaatacaaa acgtggatca ttttgcgctt ctggcgcagg aacgacgatg gaacgcatgc
     2761 gggctgcttt agaagcgatc atgccattta ccgagtcttc cagcgccacg caggtcagtg
     2821 ggtcaacgcc cagttttgct gcgcagtcga gatatacttg cggatgcggc ttgctgtaag
     2881 gcagtttttc agccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca
     2941 ctttttccag catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt
     3001 tgcataacgc aacagcttcg cgcacgcctg gtaataatgg acgtgtctct tcaaccagtg
     3061 aaatggcacg ggcaataacc cgttctacta cttcctgacg gcttggccca ttccacggtt
     3121 gccgggcgta ccaaagatcg accaccatat cgatgcgtaa acctaaggtg tccggcagct
     3181 cgttacgacg ggagatatcc acccccaggc ttgccatcac atccagttcg gctcgatccc
     3241 ataaaggttc tgagtcgata agtaatccat ccatatcaaa aattgcagca agaatctgac
     3301 gcggggttga cattacaacc tctcctttag agcattatgt gtaaggtatt aagccgtgct
     3361 tatgattaag cgcctatcat atcgccttta aagaatgggc gaaaacagat atctacaggt
     3421 aaactaagtg aaacgacgcg tcttaatcaa ggagaactca tgacgtatca acaagctgga
     3481 cgcattgctg ttttgaaaag gattttgggt tgggtgattt ttatacctgc attgatctcc
     3541 acattgattt ctttgctgaa atttatgaat acccggcagg aaaaccagga aggcattaat
     3601 gcggtcatgc tcgactttac tcatgtcatg atcgatatga tgcaggcgaa tacgcctttt
     3661 ttgaatctgt tctggtataa ctccccgacc cctaatttta atggtggcgt gaacgttatg
     3721 ttctgggtga ttttcatcct gatttttgtc ggactggcgt tgcaggattc cggtgcccgg
     3781 atgagtcgcc aggcgcgctt cttgcgggaa ggtgttgaag atcaactcat tctggaaaaa
     3841 gccaaagggg aagaggggct aacgcgtgaa caaattgagt cccgtattgt tgttccgcac
     3901 catactattt tcctgcagtt tttctcacta tatatcctgc cggttatttg tattgctgca
     3961 ggttatgtgt tcttttctct gcttgggttt atttaatcaa aaggccgcac ggagcggcca
     4021 ttatcctcaa cccatatcat gctgctaata atctatccaa tgactgctga gcaataacca
     4081 aatgctgacc gccaaataat cttgcacgat ttaacagcgt gtagagttgg taaaccggtt
     4141 gacgttcaag gaaatcggca ggtagcgggg atactgactg atagccgtca tagatttgtg
     4201 gcggctgttc agtatgcagc ggtaacatcg ccaggtcgca ctctcggtca ccccagtagc
     4261 aggcagggtc gaaaatgtac gggccatccg gacccagcgc acagttgccg gaccataaat
     4321 cgccgtgcaa cagagaaggc tgcagttgat gcgaggccag acgctgctga atatgctcga
     4381 cgatggcgtc gatattgccg aaagcgatcc ctttctctgc tgccagttcc aactgccagc
     4441 caatccgttg ttcagcaaaa aacgttgacc agcgacgttg ccaggtgttg ggctgtggag
     4501 ttgtggagag cgagttatca aaatcgaggc caaattgtgg ttggtcactc cattgatgta
     4561 aacgcgcaat ttgctgacca agaataaatg cgctatgcgc atccagtgga cgaggtggga
     4621 gataatccat caccagaaaa ctgtagtcac ggtcagcgcc tactgcccaa accttaggca
     4681 cggtgacggt tttactacgc gacagtaact ccagttggtc ggcttcggcg gtaaaaccgg
     4741 gaagcagttc cctttcatca catttgacga aaaagtcatg tcctgcatag cgcaaatgcc
     4801 atgcggcatg gacttctccg ccaggcagtt cattacgcag ttcgatttcg ccttcaccta
     4861 actgctcgct caaaagacga ctgattgcct gccacatgat gtctctccca tgttgtctgc
     4921 cagatcataa ggttagcgca agaatgcggt gtaaaaacac gagctaacgc acaccacaga
     4981 ctgattattg cccaaaaacc atttattttc tgttgctatt ccattcctcc caagtcgtga
     5041 tggtgatttc aggatctttg ccggttgcac tttgggtaag ccccgaaacc agttctctaa
     5101 tttcttcttc actttgggta ctgataagtc caaaagtgtt agtccccagc tcatggatat
     5161 ttccctcatc atccgtcatg gtgagcaaaa aaccgtcgcg agtcaggtaa ttattcaact
     5221 cgttaatttc agtcaatgta tcctcatgca acatgacggt tatgacataa cggacaagat
     5281 cgccactggc catagttcac ccctttgtta tcacatgact tttgagcaaa gtcggagaaa
     5341 cgcgttgccg acaggttggt ggtgattccc ccaatgctgg gggaatgttt ttgttagcgg
     5401 gaaaggtaag cgtaaatttt ttgcgtatcg tcatgggagc acagacgtgt tccctgattg
     5461 agtgtggctg cactccccgc agctacgcca aaacggacca tctcttcaag agaggcattt
     5521 tctgccagtt tcagtgtcat cgcgccgacc atgctgtcac cagcgccaac ggtactctgg
     5581 cttttcaccg gtggtggcac cacctgaatg cagttttcac tatcaacacc cagcgctcct
     5641 tgtggaccaa gggaaacgac aacccgtttg gccttgccgc tattaacgat ttcctgcgcg
     5701 gctttgcgga catcgtccgg ctgggtgagt tcgcgattca ccagcgcact gagttctttt
     5761 tggttaggct taaccaactc gatgttacca attgccagtg ctgcacttaa cgcttcgcca
     5821 gaactgtcga cgatgcagcg gatcccttgt ttttgcgaag cggaaatcag ttgggttaat
     5881 ttttccagct tcacacctgg cggcaggctt ccgcttatga ccaggatggc cccggattca
     5941 atttccagaa cttgctcttc aagctggcga aactcatctt catttaatgc cgcgcctggc
     6001 ataacaaaac gatactgctc accgcttgct tccacatgta catgtaaatt ctgccgggtc
     6061 cagtctttgg cttctacagt agcgacgggg acattttcat ccgccaacag tgaaaccagg
     6121 tgttcgccgg tcgcgccacc cgccgggaag atcgctgtgg cactgcctcc aagatgggca
     6181 atggcgcggg cgacgttgat gccgccgccc ccgggttcga acaccggtgc ggtacagcgc
     6241 agttttcctt cgggataaat ttgcggggta attgttgcgc tatcgagaga gggcgcaagt
     6301 gtcaacgtat agatacgtac catcatttcc tcctataggc tgatttc
//
LOCUS       DAZPBM010000207         6302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPBM010000207 DAZPBM010000000
VERSION     DAZPBM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.208"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..273)
                     /locus_tag="ACHUZQ_003817"
     CDS             complement(<1..273)
                     /locus_tag="ACHUZQ_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027409.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIV"
     gene            complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHUZQ_003818"
     CDS             complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHUZQ_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1027410.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4551..5555)
                     /locus_tag="ACHUZQ_003819"
                     /pseudo
     CDS             complement(4551..5555)
                     /locus_tag="ACHUZQ_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5540..5917
                     /locus_tag="ACHUZQ_003820"
                     /pseudo
     CDS             5540..5917
                     /locus_tag="ACHUZQ_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5956..>6302)
                     /locus_tag="ACHUZQ_003821"
     CDS             complement(5956..>6302)
                     /locus_tag="ACHUZQ_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1027411.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1571 a         1408 c         1410 g         1913 t
ORIGIN      
        1 gacaatgctg tattcttttt taaagcactc accagaaacg gtgattgtaa tgataatggt
       61 cttctcgatc gagatgtaaa tacccgtttc gttatactgt ccgtatcgga accatgagaa
      121 ctcagttgca ttccagccat gctccagaat atgtgcattt ttttgcctga tagtttcttt
      181 ttttattcgc tcatgtttaa ggcataacag gcagataaat aatgtcagaa aaagaaagct
      241 aataatgaga gcaagagcga tatagtctgt cacagggtaa tacatattct ctacggtaga
      301 agaagggccg caaacgcggc ccgggctgtt atcagaaaga gtaacggaag ttggcgttga
      361 tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc aaactcaaga ccgaagcgaa
      421 gattatcgcg aatttcggcg ttgagaccaa cattcatgag aatacgaccg tctttttcac
      481 ctttgatacg tttctcaccg gacgcatcac gcagtacggt ttcaccgttg gcaaacaggt
      541 caaactggta gccaaggccg gcgcgggctg tgactttcca gtccttaccg gagaaggatt
      601 tacccacatc aacaccggta cgcccaatca gcggattaaa gtccttatcc ttcatggtca
      661 cgctcattcc gcgatcctgc cagtcgaacc gtttacctga tacggcccca taaaccagtt
      721 ctgcctgcgg ctcaatccag gagtcttctg ttacatgata gcggtagcct gcttcagcac
      781 ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag tccagcaaag gtcgccgagt
      841 actcattatc atggtgaaca tacttactaa tcaggtcgat ataggcacca gagtcaaata
      901 tagcggaagc atacagacct gccccgacgg acttcgtctt gccactgaat gcattactgc
      961 tggcatgact gtcggtatac gtcatagtca gaccagtgaa aaggtcaagt ccatccagct
     1021 catgtttttt atccacacca atctgcacat gggtgtagtt gtcactgtat ccactagaag
     1081 ctgaacctgc tccgctcatg atgcgggccc atgcaccggc ctcgccattg gtgtcacgca
     1141 gatcccccat tcgtttattc aggttgttga cttctgtcag gaaggctttg taattaccgg
     1201 acatatacga ttttgctgca ccggaagatg ctgtattttc agccacctta tatccggtca
     1261 gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat cacaggagat atgttgctga
     1321 aacctatggc ctgagtactg gctttaaata tctctttatt gcttcctttt ggggctgtta
     1381 tcaggtcaac gtttaatcct gagttattat ttcccacttt ctgtatatcg accgacagac
     1441 tgttattgct tccggttaac tgatttttaa caatgagttt atccgattga tttacagact
     1501 ggctcagatc agcccgcata atcacagcag tattagtggc atccagtgtg tttactgtca
     1561 cagtatggaa acccgaattc tctatgttac taaacaacaa aagactgtcg cgggttttta
     1621 atgattgcag ggctgactgt ccgtttactc tccataaggt gttatacatg ctggctgagg
     1681 cagcaggagc atcaatacct ccggtataag aagtatcaaa gccacccagc aaacctgtag
     1741 ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt atactggtca acaccaaaca
     1801 gaatagatgc ttttttgtct gattttatat taccattgac ccaggcataa ttttgagcct
     1861 ttaacgtggc accatcttct gtcatattat atccatctga catatagatt gccgggctgt
     1921 atttattatt ttctgtaggg gtgcctgtta atgtcagggt tccggccagt tcaatagctt
     1981 tatctacata catttttccc tgggcggtaa cagtagctcc tttgttgata atcaggtctg
     2041 tattaattaa ggataatgca ccagtcatag taacagttga tttatcacca acagttattt
     2101 tgctgttttg agcactcaga ctgccgttga aactactttc gcctgctgaa tccccggagc
     2161 gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt tcttcctgag aacatgtcaa
     2221 tgtatgcagt tttatttccc agaacaattt gagagttttt agcctctatg tgtcctttta
     2281 cattagcatc ccggcctata ctcagggttg cgttttctaa atttaatttc cggaaattaa
     2341 atttacgact ttcccagtcg ggctgggaaa aagaggctat ctgattgttg gacttatatt
     2401 ccgtattgta acgtttattt acagtgcttt cctggtcctt tatggcagca gaatagtctt
     2461 tttgacataa aaaaggaatt ggacagttgt tattgccttc tttaaatatg gcatgttctg
     2521 tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga gatatcgttt ttgatatcga
     2581 ctgagccatc aaagatcaga ttggcattat tgttatgttg ctgggtagta ttgataatat
     2641 gatttatgct tatattacct gatacattcc catggtacat atatgcatca gtattagtaa
     2701 gtgataatgt tgattgcttc acgtttgagt tcgttacggt tgttcccgca tctgttgctg
     2761 cgattttctg aaatgactgg tcatagccgt ttagatccag tgttccgcca tttctggtaa
     2821 aatagatttc gccattaccg ctttcgctta aagcgtcttt ggcatttatt tttaccgtac
     2881 ctttacctcc ggccatgtaa attttattga aggctttttc agcactaagg atgacagtcc
     2941 cattacctat tttaaggtta tttccctgtg ctatttttac atccagagta ccactaccta
     3001 ttttatgcag attatctccg gcaacgccct taacattcca ttcaacggtg gtattattat
     3061 caatatctat cccggcacct ttataagact tatcttttcc gtacacttta tatgtctggt
     3121 tcttatcgaa tataagcccg ccaatgccaa gatcggtatc ctgcttgagc gttaattccc
     3181 ctcctcctga gacagacagg tccttatctt ttataatcga tgataactct tgttttttac
     3241 catccagctg catagtgttt gtgaaattct tttttaaatt gtttaccaga atattattaa
     3301 ataacgagta gtttgtccag gctatattgg tactactgag aaaaccaact gtactggtaa
     3361 caccatggaa aacccatttt tgttgttctt tatcatatag ataagaacca gaaccactat
     3421 cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt ataaatgtaa ctcttgtttt
     3481 tccagtccag attaaaaaga cttgcgctta acaagtcggg attatagttt tgtccaaaca
     3541 gatattgttt gccgtttaat atcgttgagg ttgttccggc acctgctctg aagccaatta
     3601 tttgttcctt accgttgtag tttacgccat atcttttcat cgcatcttcg ggagaaagtg
     3661 agaagtcggc actctccgta acccctgttg tttccacgac gaatttattc agacgatgaa
     3721 ccgaaaagtc gccagatgat actctgttac ttgctttata tgctgtctga tcccatgact
     3781 gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc
     3841 caattgatgt tgttgcacct ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg
     3901 gaaaatgaaa tatctttcca tctttcatca ttaattgaac atttgttgca cccggtctga
     3961 actctcctct gttttgagca aggtcaagat aatcgcgggt ccatatatta tttatatcca
     4021 gcattgctgc aaatgctgtt tcgccataac ttccatatat tgaggataaa ataataccga
     4081 ggataacttt ctttcttgat gtaccgacac taactttccg ggtcagttca gaaacagcaa
     4141 ctaatccacc tgtaatatga ctatatttca gtgaataaat tttattcata cgtgaactcc
     4201 gatatttctg agttgtggtt atgggatagc atttaattac aacggggaac tatcagtcat
     4261 ggtacgtaac tgataagggt ataatgataa tcactgtcag ttatatttgt atgacagatg
     4321 taacaaaatg aaaaaaaaag ataagcacaa gtgattgatt ggtcagatat tgatcacttg
     4381 gtgtttgtat tttgaacttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     4441 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     4501 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     4561 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     4621 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     4681 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     4741 gcgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     4801 tccacatcaa atgcggtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     4861 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacggggg
     4921 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     4981 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5041 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5101 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5161 tcagtgatca gtgccgcgcg ggcaaaggcc tgtgatgtca ggcggctttt gtccacctcc
     5221 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5281 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     5341 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     5401 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     5461 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     5521 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     5581 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     5641 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     5701 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     5761 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     5821 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     5881 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     5941 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6001 aatatcccac ataaatccag ttaactcacg agccaccgcc gtaatggcta cttgtgattt
     6061 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6121 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6181 ttttgcaggt gtacgataag accaagcggc ttcgtaaagc agacgtctca gttcgctgtt
     6241 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6301 ca
//
LOCUS       DAZPBM010000208         6192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPBM010000208 DAZPBM010000000
VERSION     DAZPBM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.209"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..78)
                     /locus_tag="ACHUZQ_003822"
     CDS             complement(<1..78)
                     /locus_tag="ACHUZQ_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027412.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVIL"
     gene            complement(291..1961)
                     /gene="betA"
                     /locus_tag="ACHUZQ_003823"
     CDS             complement(291..1961)
                     /gene="betA"
                     /locus_tag="ACHUZQ_003823"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFR1027413.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1975..3447)
                     /gene="betB"
                     /locus_tag="ACHUZQ_003824"
     CDS             complement(1975..3447)
                     /gene="betB"
                     /locus_tag="ACHUZQ_003824"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFR1027414.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3461..4048)
                     /gene="betI"
                     /locus_tag="ACHUZQ_003825"
     CDS             complement(3461..4048)
                     /gene="betI"
                     /locus_tag="ACHUZQ_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFR1027415.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4187..6139
                     /gene="betT"
                     /locus_tag="ACHUZQ_003826"
                     /pseudo
     CDS             4187..6139
                     /gene="betT"
                     /locus_tag="ACHUZQ_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
BASE COUNT         1268 a         1713 c         1713 g         1498 t
ORIGIN      
        1 aaggataaca cgaatctttt tcttcagata aaataaaatc tcaatcagat cgatatcctg
       61 tttgatttgt tcactcataa tatatccaga aaataaaatc tgccgcagat aaggttgtat
      121 taatagtctg tatcaggaat gttcggggta aatatcagca aaaaaccagc atcatgaata
      181 ctggatatga agcatgagag ttacctcagt gtttatataa ggattcggtc cccctctctg
      241 gaacggtaac tctcaatctg atcggttcct gcgttagttc acatcacgac tcattttttc
      301 gctctcaccg gcatcccatt tgccacaaaa tatcccgccg tgctcctcgg cagcgcttcc
      361 tgtccacgaa tcatatccgc tattttctcg ccaatcataa ttgtcgtggc gttcaaattc
      421 ccggtgataa tctgcggcat aattgacgca tccaccacgc gcaggccttc cagcccatgc
      481 acgcggcctt cgccgtcaac cacggacatc tcgtcgtaac ccattttgca ggtaccgcac
      541 ggatggaagg cggtttcggc gtgattacgc acgaactcat cgagctgctc atccgtctga
      601 cattccgtgc cggggctgat ttcgcggcca cgatactgat ccagcgtcgg ttgatgcatg
      661 atctcccggg tgatgcgaat tgcgtcgcgg aactcctgcc agtcctgctc gtgtgacatg
      721 tagttaaaca gaattgccgg atgctggtgc gggtcgcggg atttaatccg cacatgccca
      781 cggcttggcg agcgcattga gccgacgtgg cactggaaac cgtgctcctt caccgcattc
      841 gagccgttat agttaatcgc tactggcagg aaatggtact gaatattcgg ccacgcaaat
      901 tcctctcggc tgcgaataaa tccgcctgct tcaaagtggt tgctggcacc aacgccagtg
      961 ccgccaaaca gccactccgc accgattttc ggctggttcc accactgcag ggcagggtag
     1021 agggaaaccg gttctttgca ctcatattgc agatacatct ccagatgatc ctgaagattt
     1081 tcgccgacgc cgggtaattc atgcaccagc ggaatatcaa actccgccag cagttcagcg
     1141 ttgccgacgc cggagcgttg caggatctgc ggtgaggcaa tcgcgcctgc acataacagc
     1201 acttctttgt tggccgttgc gcgggttggg atggtgctgt cgccttccag ccattcgacg
     1261 cccaccgcgc gtttgccgtc aaaaatgatg tgatcggtca tagcgtgagt acgaatggtc
     1321 aggttagggc gcgatttggc ctgatcgaga taaccgcgcg cggtactggc gcgacggccc
     1381 tgcggcgtga cggtgcgatc catcggacca aaaccttcct gctgataacc gttgagatcg
     1441 tccgtgcgcg ggtagcccgc ctgcacgccc gcttcaatca tcgcttcaaa cagcggattg
     1501 acgccgggtt tggaggtagt gacgctcacc gggccatcgc caccgtggta gtcgttctcg
     1561 cccacatcgc gagtctcggc cttgcggtag tagggcaggc agtcgagata actccagttc
     1621 tccagaccgg gttcttgcgc ccagttatcg agatccagcg cattgccacg gatgtagcac
     1681 atgccgttga tcagcgacga tccacccagg cctttaccgc gtccgcactc catgcggcgg
     1741 ttattcataa acggttcagg ctctgtttca taggcccagt tgtagcgttt gccctgtagt
     1801 gggaacgcca gcgctgcggg catctgggtg cggaagtcaa agcgatagtc cgggccgccc
     1861 gcttcaagca gcagcacgga ggtattcgga tcttcagtca gacgggtagc gagaacgttg
     1921 ccggctgagc cggcaccaat aatgatgtag tcaaattgca aataaacctc ctggttagaa
     1981 tatggactgg aatttagcca tctcaacctg gatggacttc acctgggtgt aactctggag
     2041 cgtcatcacg ccgttctcgc gaccaatgcc ggagtgtttg tagccgccaa cgggcatctc
     2101 tgccggggat tcgccccagg tgttgatcca gcaaataccc gcttccagct gatgaatgac
     2161 gcgatgcgcg cggttcaggt ccgccgtcac gatgcccgcc gccaggccgt agtcggtatc
     2221 attggcgcgg cgaatcactt cgtcctcaga ttcataagtg agaagcgaca tcaccggacc
     2281 gaagatctct tcgcgcacga tggtcatttc gtcgcggcaa tcggtgaaca cggtcggtgc
     2341 aacccatgcg ccgttatcga agccatcgcc tttcagtaca tcgccgccgc acagtacgcg
     2401 cgcgccttcc tctttgcctt tggcgatata gcgcagcacg ttatcgcgat gcgggaagct
     2461 gaccagcggg ccgaagttag tttgcggatc gaaaacgtcg cccgcgcgaa tgcgctcaac
     2521 gcgcgccaga attttctgct caaatgcggc tttgcatttc gccggaacga agacgcgggt
     2581 gccattggta cacacctgac cggagctgaa gaagtttgcc atcatggcga tatcggcggc
     2641 gagatcgaga tccgcatcat cgaaaacgat cagcggtgat ttaccgccca gttccatggt
     2701 cacttctttc agggaagagg ccgccgagtt agccatcact tttttgccgc tggcgacacc
     2761 gccggtaaat gacactttgg caatgcccgg atgctcggtc agatattgcc cggtctccgc
     2821 gcccaccccc ggcaacacgt taaatacgcc gtccggcagg cccgcttcgc tgtaaatttc
     2881 agccagcttt aacgcggtaa gcggggtgac ttcgctcggt ttgaaaatca ttgcgttgcc
     2941 tgccgccagc gccggggcgg atttccacag ggcaatctga atcgggtagt tccatgcgcc
     3001 aatccccgcc accacaccca gcggttcgcg gcgggtataa acaaatgacg tctcacgcaa
     3061 cgggatctgg ctgccttcca gcgccgggat cagcccggcg tagtactcca gcacgtccgc
     3121 accggtaacg atatcgacgg ttgaggtttc cgaatatgct tttccggtat cgagggtttc
     3181 cagttttgcg agttcgtcat tgcgttcacg cagaatatca acggcccgac gcagaatacg
     3241 cgagcgctcc atagcggtca tcgccgccca gattttttgc ccctgttggg cactcttcac
     3301 ggcgcgatcg acatcctcac gcccggcggc ctgcacggtc gccagcacgt taccgttggc
     3361 cgggttaatg gtctcgaagg tgcgaccgct ggtggcggag gtataaccac catgtatata
     3421 aagctgctgt tctgccattc gggacatcac gtctcctcgg ttaatcggtg ggtaaatgct
     3481 gagtaataaa gtgacgggtc agcgagtgtg cgagaggttt atccagcggt ttgccgctta
     3541 gtgccgcgcg cagccataat ccgtcaatca gcgcggcaag gccgtagccc gcttcctgtg
     3601 cctgttcgcg cggcaattcg cggcgaaact cgctcaccag attcgacagc aagcggcggc
     3661 tgctgacctg ctgtaaacga tagagcatcg gctgatgcat actgctggcc cagaacgcca
     3721 gccaggcttt catcgccgca ctgctcacct gagtttcatc gaagtttccg gcaacaatcg
     3781 cctgtaatcg ctgctctgca ctgccctgcg gaagtgcatg taatcgattc aaaaccgcgt
     3841 cacgcagctg actggtgata tcgcgcatgg ttgcttccag cagaccattt ttgtccctga
     3901 aatagtggct gatgatcccc gtagaaacgc ctgcacggcg ggcgatctgc gcgatcgttg
     3961 catcgtgcat gcccacttca tttattgctt ccagtgtggc gtcgatcagt tgtctgcgcc
     4021 ggatcgactg catccccaat ttgggcattt tcgccactcc attcatcagc gttgtttatc
     4081 tattaaagcg gttattgatt ggacgttcaa tataaaatgt gtcgtaattg ttacgaattt
     4141 gattttaaat ggtaacaata acagtgggga tactggatga cagacctttt ccctgacaac
     4201 gatcctgttt cgcgacttct cggccctgtg gattggccgc acgctggact gggtttctaa
     4261 aaccttcggt tggtactatc tactggcggc aacgctctat attgtctttg tggtctgtat
     4321 cgcttgttcg cgttttggtt cggtgaagct cgggccagaa caatccaaac cggaattcag
     4381 cctgctgagt tgggcggcga tgctgtttgc tggcgggatc ggtatcgacc tgatgttctt
     4441 ctccgtagcc gaaccggtaa cgcagtatat gcagccgccg gaaggcgcgg gacagacgat
     4501 tgaggccgcg cgtcaggcga tggtctggac gctgtttcac tacggcttaa ccggctggtc
     4561 gatgtatgcg ctgatgggca tggcgctcgg atactttagc tatcgttata atttgccgct
     4621 caccatccgc tcggcgctgt acccgatctt cggtaaacgg attaacgggc cgataggtca
     4681 ctcagtggat attgcagcgg tgatcggcac tatcttcggt attgccacta cgctcggtat
     4741 cggtgtggtg cagcttaact atggcttgag cgtactgttt gatattcccg attcgatggc
     4801 ggcaaaagcg gcactgatcg ccttgtcggt gataatcgcc acgatctctg tcacctccgg
     4861 tgtcgataag ggcattcgtg tgttatcgga acttaacgtt gcgctggcgc tgggattgat
     4921 cctgttcgta ttgtttatgg gcgacacctc gttcctgctt aatgcgctgg tgctgaacgt
     4981 tggcgactat gtgaatcgct ttatgggcat gacgctcaac agttttgcct tcgaccgtcc
     5041 ggttgagtgg atgaataact ggacgctctt cttctgggca tggtgggtgg catggtcgcc
     5101 gtttgtcggc ttgttcctgg cgcgtatctc gcgtgggcgt accattcgcc agttcgtgct
     5161 gggcacgttg attattccgt ttaccttcac gctgttatgg ctctcggtat tcggcaatag
     5221 cgcgctgtat gaaatcatcc acggcggcgc ggcatttgcc gaggaagcga tggtccatcc
     5281 ggagcgcggc ttctacagcc tgctggcgca gtatccggcg tttaccttta gcgcctccgt
     5341 cgccaccatt actggcctgc tgttttatgt gacctcggcg gactccggtg cgctggtgct
     5401 ggggaatttc acctcgcagc ttaaagatat caacagcgac gcccccggct ggctgcgcgt
     5461 cttctggtcg gtggcgattg gtctgctgac gctcggcatg ctgatgacca acgggatatc
     5521 cgcgctgcaa aacaccacgg taatcatggg gctgccgttc agctttgtga ttttcttcgt
     5581 gatggcgggg ttgtataaat ctctgaaggt agaagattac cgccgtgaaa gtgtcaaccg
     5641 cgataccgcg ccgcgaccgc tggggcttca ggatcgcctg agctggaaaa aacgtctatc
     5701 gcgcctgatg aattatccgg gcacgcgtta cactaaacag atgatggaga cggtctgtta
     5761 cccggcaatg gaagaagtgg cgcaggagct gcggttgcgc ggcgcgtacg tggagctaaa
     5821 aagcctgccg ccggaagagg gacaacagtt ggggcatctg gatttgttgg tgcatatggg
     5881 cgaagagcaa aactttgtct atcagatttg gccgcagcaa tattcggtgc cgggctttac
     5941 ctaccgcgca cgtatcggta aatcgaccta ctaccgactg gaaaccttcc tgttagaagg
     6001 cagccagggc aacgacctga tggactacag caaagagcag gtgatcaccg atattcttga
     6061 ccagtacgag cggcacctta actttattca tctccatcgt gaagcgccgg gccatagcgt
     6121 gatgttcccg gacgcgtgat tgtttttgca taaaaccgcg ccatgtctgc atatggcgcg
     6181 ggaatcattg ac
//
LOCUS       DAZPBM010000209         6153 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPBM010000209 DAZPBM010000000
VERSION     DAZPBM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.210"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            19..1329
                     /gene="uidC"
                     /locus_tag="ACHUZQ_003827"
     CDS             19..1329
                     /gene="uidC"
                     /locus_tag="ACHUZQ_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1027416.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(1510..3018)
                     /locus_tag="ACHUZQ_003828"
     CDS             complement(1510..3018)
                     /locus_tag="ACHUZQ_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1027417.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(3119..4294)
                     /gene="manA"
                     /locus_tag="ACHUZQ_003829"
     CDS             complement(3119..4294)
                     /gene="manA"
                     /locus_tag="ACHUZQ_003829"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1027418.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            4493..6139
                     /gene="fumB"
                     /locus_tag="ACHUZQ_003830"
     CDS             4493..6139
                     /gene="fumB"
                     /locus_tag="ACHUZQ_003830"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1027419.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         1543 a         1440 c         1569 g         1601 t
ORIGIN      
        1 aacatcaaag gtgcaactat gagaaaaata gtggccatgg ccgttatttg cctgacggct
       61 gcctctggcc ttatctctgc ttatgcggcg caactggctg acgatgaggc gggactacgc
      121 atcagactga aaaacgaatt gcgcagggcg gataagccca gtgctggcgc gggaagagat
      181 atttacgcat gggtacaggg aggattgctc gatttcaata gtggttatta ttccaatatt
      241 attggcgttg aaggcggggc atattatgtt tataaattag gtgctcgtgc tgatatgagt
      301 acccggtggt atcttgatgg tgataaaagt tttggctttg ccctaggggc agtaaaaata
      361 aaacccagtg aaaatagcct gcttaaatca ggtcgcttcg ggacggatta tagttatggt
      421 agcttacctt atcgtattcc gttaatggct ggcagttcgc aacgtacatt accgacagtt
      481 tctgaaggag cattaggtta ttgggcttta acaccaaata ttgatctgtg gggaatgtgg
      541 cgttcacgag tatttttatg gactgattca acaaccggta ttcgtgatga aggggtgtat
      601 aacagccaga cgggaaaata cgataaacat cgcgcacgtt cttttttagc cgccagttgg
      661 catgatgata ccagtcgcta ttctctgggg gcatcggtac agaaagatgt ttccaatcag
      721 atacaaagta ttctcgagaa aagcataccg ctcgacccga attatacgtt gaaaggggag
      781 ttgctcggct tttacgcgca gctcgaaggt ttaagtcgta ataccagcca gcccaatgaa
      841 acggcgttgg ttagtggaca attgacctgg aatgcgccgt ggggaagcgt atttggcagt
      901 ggtggttatt tgcgccatgc aatgaatggt gccgtggtgg ataccgacat tggctatccc
      961 ttttcattaa gtcttgatcg taaccgtgaa ggaatgcagt cctggcaatt gggcgtcaac
     1021 tatcgtttaa cgccgcaatt tacactgaca tttgcacaga ttgtgactcg cggctatgaa
     1081 tccagtaaac gagatgtgcg gattgaaggc gcgggtatct taggtggtat gaactatcgg
     1141 gtcagcgaag ggccgttaca agggatgaat ttctttcttg ctgccgataa agggcgggaa
     1201 aagcgcgatg gcagtacgct gggcgatcgc ctgaattact gggatgtgaa aatgagtatt
     1261 cagtatgact ttatgctgaa gtcagaaata acgccggaga gaaaaacctc cggcgtttca
     1321 gattgttgac aaagtgcgcg ttttttatgc cggatgcggc gtaaacgcct tatccagcct
     1381 acaaaaactc ataaattcaa agtgttgcag gaaaaggtag gcctgataag cgtagcgcat
     1441 caggcaatct ctggtttgtt ttcagatgaa aacgccgaag tgaaaattct ccggcgtttt
     1501 ggccgtgaat tactgctgcg gaattgccgg tacagccgga acgttaagag ctggcatcgc
     1561 aaacatacca acgaaatctt ccagtggcat tttctgcccg tttaacgtta tctgaccgtt
     1621 agtatattgc aggctggtgg tgatggtatt gtcctgcaag gtggtcagac ggaacatctg
     1681 ccccattgct gatgcacctt caacttgctg tttcgccagt tttttcgctt gatcttcctg
     1741 ataaccttcc agcttcgcta cctgagtcat aaactcagtt gccatatcca ccggaatggt
     1801 cagtttcgca tccagagatt taaccgaacg atctacttcc tgcgccagcg tttgcggcgc
     1861 ttctttagtc gttgccggat ctttcaggaa cagcgacaga ttcagcgcac tttcaccctg
     1921 actgtttttc cagcttagcg gcgcgatagt aatcaccgga tcgcctttca acatcagcgg
     1981 cagggcgcta aagaaggctt ccgtcacttt ctcctgataa agttcgggat tgttggcaat
     2041 ttctggctgt gccagcagcg cctgagtttg cgcgttatat tgctggctaa actgatgcca
     2101 ggcttcacca tcaatctggc cgacttttaa agtcagcttg ccgctgccca gatcctgatt
     2161 ctgtaccttc aggctgttta gcgagtaatc cagttggcta ttgatcgttt taccgtcatt
     2221 gaccagatcc gatttaccgc tgatctccat gccttccagc agtgccagtt ctttgccttc
     2281 cactgaaatg gtcatttttt ccagtgacag tttttggttt cctacgcgct caccaaaact
     2341 tgccagcgtg ctggaaccgt cggttttcag attattaaag gtcaactgca ctttctggtt
     2401 gtattcgtta actgcgtcta tccgaccact ttgcgcctcc ccggaaaggg agatggcttt
     2461 gccgtctctg tcagcattta actggaactc gccgccgcta aaggcgactt tttcatcctt
     2521 ttgctcgtaa ttcagtggct tgagcgaaat atcggaactg gaatcaccgc tgtaaccaat
     2581 gcgcgagtta atctcaaaag gcgtttcacc ttttgccata tcaaacagtg gtttgcttac
     2641 ttcgttatta accagcgtgg tttgaattga tgccatcgac gggatcaggt tcagtttttt
     2701 aagctgggca agcgggaagg gaccatgatc aaccgattcg ttgaagatga cgctctgacc
     2761 gcttttaatc cacggatttt ctttcccggc aatgggtttc accaacagtt gcaactggct
     2821 gctgaatacg ccgcgatgat agttttgata actcacttcc aggttggatt caggagctgt
     2881 cagtttgagt tgcgcgttcg cctgcgcgac catgtcttcg agatgggttt caatcttctt
     2941 gcctgtatac catgcgccgc ctgtccagac tacgcctagc gcaacaatga cgcctaccgc
     3001 taccagcgat ttattcataa tgattatcca taaaatgaaa tcaggcggac tggccgcctg
     3061 aaggtgttat aagcctttaa taagcttagc aagagatgtt aattttttca gtaagctctt
     3121 acagcttgtt gtaaacacgc gctaaacggc cgtggccttt gacagtcacc ggtgattcgt
     3181 tggcggcaat aaacgctgat tcacccggtt taagctgtaa ctgctgagaa cctttccaca
     3241 acgttgcatc gccttcgacg cagaacaaaa tggcggcact ctgctggcta atggtggttt
     3301 ctttatcact aaggtcatgc agcgagaagg caaaatcatc cactggaatc gggaagtcca
     3361 gttctgcacc ttgtttcacc ggctgggtca acaactggtt agccggtttg gcttcgaatt
     3421 tcacattggc aaccagttcc ggaatatcaa tgtacttagg cgtcagaccc gcacgcagca
     3481 cgttatcgga gttcgccatc acttccagcg ccacgccttg caggtaagcg tgcggtgttt
     3541 cagcgaacag gaacatcgct tcgccaggat tcaatttcac cacattcagt aacagcgggg
     3601 agaacaggcc actgtcttcc gggtaaaatt cagaaattaa acgaatcgtt tgccacggtt
     3661 caccctgctg actgtcgagc gccgatttta aaatcgccag cgcgcgggat ttttcttcac
     3721 cctgcatatt caacaggctg gcgaacagtt cgcttaaacg ttcggcatta ggctgttgta
     3781 aaaagtgagc aattgccgga tgtgcacctg cgactggctg gagtagggag acaatctcgg
     3841 aaaactcacg aaacgcgttc atcgcaagga aaggcgtcag cgcaaaaacc agctccggct
     3901 tgtggttagg atctttatag ttacgctcgg cggcatccat cgggatacct gcggcatttt
     3961 ctttggcaaa accgatttca gaattgcgtt tgtttggatg aacctgaatg gagagcggct
     4021 gtgctgcgca taatactttg aacaggaaag gcagttcgcc aaagcgtttg gcaacggcct
     4081 ctccgagcag agtcgattta tcactctcaa tcacatcacg cagtgaaacg atatctccgg
     4141 cggcattctg cactcgtgaa ctgcttttcg gatgtgcgcc catccacagc tcggccatcg
     4201 gctggctgga cggattttcc ataccataaa gttcagtcaa cgccgttttg ctgccccagg
     4261 catagttttg cactgagtta atgagttttt gcatgatcaa tccctgtttt aatgtggaaa
     4321 ttaatcccac tattaaagca agaatcctac gggaagtaac ctggagccgc aaaaagtcgt
     4381 actagtctca gtttttgtta aaaaagtgtg taggatattg ttactcgctt ttaacagggc
     4441 aacggaacac ccgcccagag cataaccaaa ccaggcagta agtgagagaa caatgtcaaa
     4501 caaacccttt cattatcagg ctccttttcc actcaaaaaa gatgatactg agtattacct
     4561 gctaaccagc gaacacgtta gcgtatctga atttgaaggg caggagattt tgaaagtcgc
     4621 acccgaagcg ttaactctgt tggcgcgtca ggcgtttcat gatgcgtcat ttatgctgcg
     4681 tccggctcac caacaacagg tggccgacat tctgcgtgac ccggaagcca gcgaaaatga
     4741 taaatatgtg gcgctgcaat tcctgcgcaa ctccgacatc gcggcgaaag gtgttctgcc
     4801 aacctgtcag gataccggca ctgcgattat tgttggtaaa aaagggcagc gtgtatggac
     4861 cggtggctgt gatgaagcgg cgctggcgcg cggtgtctat aacacttata tcgaagataa
     4921 tttgcgctac tcgcaaaacg cgccgctgga tatgtataaa gaggtgaata ccggcaccaa
     4981 tctgccagcg cagatcgatc tttatgccgt tgatggcgac gagtacaaat tcctctgtat
     5041 cgccaaaggt ggtggttcgg caaacaagac gtatctctat caggaaacca aagcgttact
     5101 aacgccgggg aaactgaaaa attacctggt tgagaagatg cgcacgctgg gtacggcggc
     5161 ctgtcctccg tatcatattg cgttcgtaat tggtggaact tctgcagaaa ctaaccttaa
     5221 aacagtgaaa ctggcttccg ctaaatacta tgacgaactg ccaacggaag ggaatgagca
     5281 cggtcaggca ttccgcgatg tggaactgga aaaagaattg ctgatcgaag cgcaaaatct
     5341 cggtctgggt gcgcagtttg gtggtaaata cttcgctcac gacatacgcg tgattcgcct
     5401 gccacgtcac ggcgcatcct gtccggttgg tatgggtgtt tcctgctctg ctgaccgtaa
     5461 tatcaaagcg aagatcaacc gtcaggggat ctggatcgaa aaactggaac ataatccagg
     5521 taaatatatc cctgaagagc tgcgtaaagc gggagaaggc gaagcggtgc gcgttgacct
     5581 taaccgtccg atgaaagaga tcctcgcaca gttgtcgcag tatcccgttt ctacacgctt
     5641 atcgcttaac ggcacgatta tcgtcggtcg tgatattgct cacgccaaac tgaaagagcg
     5701 gatggataac ggtgaagggc tgccgcagta cattaaagat catccgattt actacgcggg
     5761 tccggccaaa acgccggaag gttatgcctc cggttctctt ggcccaacga ccgccggacg
     5821 gatggattct tatgtcgatc aactgcaagc gcagggcgga agtatgatca tgctggcgaa
     5881 aggcaaccgc agccagcagg tgacggatgc ctgtaaaaaa cacggcggct tctaccttgg
     5941 cagtatcggt ggtccggccg ctgtattggc gcagggaagt attaagagcc tggaatgtgt
     6001 tgaatatccg gaactgggaa tggaagccat ctggaaaatt gaagtggaag atttcccggc
     6061 gtttatcctt gtggatgata aaggaaatga cttcttccag cagatacaac tcacacagtg
     6121 cactcgctgt gtgaaataaa cagagccgcc ctt
//
LOCUS       DAZPBM010000210         6135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPBM010000210 DAZPBM010000000
VERSION     DAZPBM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.211"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1255)
                     /locus_tag="ACHUZQ_003831"
     CDS             complement(<1..1255)
                     /locus_tag="ACHUZQ_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR1027420.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="ACHUZQ_003832"
     CDS             complement(1412..1621)
                     /locus_tag="ACHUZQ_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027421.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="ACHUZQ_003833"
     CDS             complement(1644..2354)
                     /locus_tag="ACHUZQ_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1027422.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="ACHUZQ_003834"
     CDS             complement(2870..3193)
                     /locus_tag="ACHUZQ_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027423.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYGDNILVTD
                     SKVVH"
     gene            complement(3372..3572)
                     /locus_tag="ACHUZQ_003835"
     CDS             complement(3372..3572)
                     /locus_tag="ACHUZQ_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027424.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(3572..3844)
                     /locus_tag="ACHUZQ_003836"
     CDS             complement(3572..3844)
                     /locus_tag="ACHUZQ_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1027425.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(3847..4125)
                     /locus_tag="ACHUZQ_003837"
     CDS             complement(3847..4125)
                     /locus_tag="ACHUZQ_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027426.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(4126..4683)
                     /locus_tag="ACHUZQ_003838"
     CDS             complement(4126..4683)
                     /locus_tag="ACHUZQ_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027427.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT         1747 a         1276 c         1069 g         2043 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc catacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttctttgatg agcaggtctg atacatgctt aatcatttta
     3541 tctgtcaaat attcgaattt aatacataac attaaatctc caggagtaaa ttgtttgggt
     3601 ggcaatttct tttattacca tccctgaaga taatcttttt agttgcctga acttcatgat
     3661 gcaaaaacca gatgacatac ttcagataat aatcacgagt gccataacga atcattggat
     3721 taccgtaagc atcatctctt gtcttaacct ctccaccatc aatccattca agcattcctg
     3781 tctcataatt aatcatgaag taagttgcaa agaaccgttt aatatcatct cgccattgct
     3841 tcataatcag acctcctgag acagtttgtg aattttgata cttagtaacc ttgctaaatc
     3901 cagtccaaaa gcatttaagg atttcagact atcaaggtca tcttgcttaa tcctgatatg
     3961 gtgtcttgca gcctctaagc gactattaag caaatcctgt tcgttaatat cacggttaat
     4021 tgcaagcttc atcactgctg agtgtggcag acctgtaatg tttttgattc ggttcagttc
     4081 atcaaaggct gtctttgtaa ctctggttaa tgtgtgttta ttcatttaaa aatcctctgt
     4141 aactaaatag gggtcaaact cgaaatctgg ttgctcgttt tccattggct caatattccc
     4201 aacggcaata tgatgttcaa caatgccaat taatttcgcg cacatcacag caagcaaagc
     4261 gcaatattta tcgcttggta attcgtaact aagtctggtt gcctgtcgct gaagatgatt
     4321 tgtcacggtg gatgcgttaa tttcgtcaag attctttagc agataaaata gttttttgtt
     4381 gtatttcggg tggccttcac ttctacagaa cccaataaaa acatcatcaa gcttgcttgg
     4441 taactgctcg taactgtcaa agttagccag ataattttta atagtattct ttgttgtttt
     4501 gcgtactggc ataaatctgt ctcgtggcgt aatgctacga atgaccgctt caatgttctt
     4561 acgttcggat ttgctaaagg ttgggacttc gtagctggtg gggttgtttg tgtaagcgta
     4621 ggagttgtgc ttgttgcgtt ctcttgatgc ctgaagcatc gctctaaatt ctgaatactg
     4681 cattcatgtt ctgactctca taagatgtta ttattctgtt aacctatata tacaatatta
     4741 gcattttcaa agactaatgt caagtatttt taacattttc gtaacatctg tggtataatg
     4801 ggtttatggg gagagtaaaa tatgaggcgt ggctatgtat ttggtcgcat attttgcatt
     4861 gcacacagac ctcttcaatc gccctgtaac gagttaaacg acctcttcag gtcaactcat
     4921 aaggtcaggt actttttgtg tcagaatgcg attgagagcg tcttacaagg aaaactaatg
     4981 gtaaatctcc ctcctgagct ggggtcacct gatgtatgga ctggtggagg tattgggctg
     5041 attgcaggcc tgctacttcg ctacgtcctc gtcagagtgc ttgttgtctt taaactgttc
     5101 ggtcgtacat ggacgcttga ccttaaggat aacgaccaca cagacccccg ttaaactcct
     5161 tcaaaacccc tgcacgagat gacagataga agtctatggt acaggggtgt ctttagcatt
     5221 ttgtgctatc tattaaaaaa tagcttgaca gaaccggaaa gctgtatcta tcaagcattc
     5281 acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa ttagccatat
     5341 atagagaact gacaagtctc ttcacagaca actctgcaac cttaacagta acttctaaaa
     5401 atgcttattt ttcaacgagt tgtaatcacc caatatataa ggctgtccag taccttcaca
     5461 gataacttca agccctctta accgccccac taatcgggag aagagcgata accccagaga
     5521 gggaagctcc tttcttaacc gacctcttaa acgaccttta cagaccttgt tcaaggtaat
     5581 cacaatagtt tttcttccct cggtttcctg tgttcctcgg ctggctattg agatttgaaa
     5641 gacctctgta agcgctctct tagcgagaga aaaatcattc atagtgaccc caagcgaagc
     5701 gcccctctaa aaaaagattt tttagctatt ttgtttaaaa gcatatcaaa cgatacattt
     5761 tattgattta ggtcaaagac aaaagcattg acaaaatggt aagattgttc acatgacctt
     5821 acttatttaa gagactttga aacagcctgt ttaacaggtc actacctctg gtcttatcgt
     5881 tcccttcttt ctaggtggtt atctgttaag agtttttcag tttttattct taagataatt
     5941 gttaagatga tactctccct tcgggttata tcgtatctct tctcccgtta aatatccctt
     6001 cacagtcaga ctaaataaca cctgtgtagt tcttggtaga atctttcaag gtaacttagc
     6061 tctgagttta tgaagttgac ctatgagata gactctaccg aatctgttag tcaattgttg
     6121 cttaggtgct gctaa
//
LOCUS       DAZPBM010000211         6120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPBM010000211 DAZPBM010000000
VERSION     DAZPBM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.212"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHUZQ_003839"
     CDS             <1..358
                     /locus_tag="ACHUZQ_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1027428.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHUZQ_003840"
     CDS             complement(398..1069)
                     /locus_tag="ACHUZQ_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR1027429.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHUZQ_003841"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHUZQ_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1027430.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHUZQ_003842"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHUZQ_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1027431.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHUZQ_003843"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHUZQ_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1027432.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHUZQ_003844"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHUZQ_003844"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1027433.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHUZQ_003845"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHUZQ_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1027434.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHUZQ_003846"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHUZQ_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1027435.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="ACHUZQ_003847"
     CDS             complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="ACHUZQ_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1027436.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1496 a         1609 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
//
LOCUS       DAZPBM010000212         6120 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPBM010000212 DAZPBM010000000
VERSION     DAZPBM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.213"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            62..838
                     /gene="exuR"
                     /locus_tag="ACHUZQ_003848"
     CDS             62..838
                     /gene="exuR"
                     /locus_tag="ACHUZQ_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1027437.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1078..1161
                     /gene="yqiM"
                     /locus_tag="ACHUZQ_003849"
     CDS             1078..1161
                     /gene="yqiM"
                     /locus_tag="ACHUZQ_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1027438.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1183..1845
                     /gene="yqjA"
                     /locus_tag="ACHUZQ_003850"
     CDS             1183..1845
                     /gene="yqjA"
                     /locus_tag="ACHUZQ_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1027439.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1849..2232
                     /gene="mzrA"
                     /locus_tag="ACHUZQ_003851"
     CDS             1849..2232
                     /gene="mzrA"
                     /locus_tag="ACHUZQ_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1027440.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2379..2747
                     /gene="yqjC"
                     /locus_tag="ACHUZQ_003852"
     CDS             2379..2747
                     /gene="yqjC"
                     /locus_tag="ACHUZQ_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR1027441.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2785..3090
                     /locus_tag="ACHUZQ_003853"
     CDS             2785..3090
                     /locus_tag="ACHUZQ_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1027442.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3093..3497
                     /locus_tag="ACHUZQ_003854"
     CDS             3093..3497
                     /locus_tag="ACHUZQ_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1027443.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3487..3786
                     /locus_tag="ACHUZQ_003855"
     CDS             3487..3786
                     /locus_tag="ACHUZQ_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1027444.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3972..4364
                     /locus_tag="ACHUZQ_003856"
     CDS             3972..4364
                     /locus_tag="ACHUZQ_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1027445.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4434..5419
                     /gene="yqjG"
                     /locus_tag="ACHUZQ_003857"
                     /pseudo
     CDS             4434..5419
                     /gene="yqjG"
                     /locus_tag="ACHUZQ_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5712..6077
                     /locus_tag="ACHUZQ_003858"
     CDS             5712..6077
                     /locus_tag="ACHUZQ_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1027446.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1530 a         1511 c         1543 g         1536 t
ORIGIN      
        1 ggcataccca tgaataaagt ggtatgataa cttcgtccgc attccactcc gggagacgca
       61 tatggaaatc actgaaccac gccgtttgta tcaacaactt gccgctgacc tgaaagagcg
      121 cattgaacag ggcgtctatc tggtgggtga taaactgcct gccgaacgct ttattgccga
      181 tgaaaagaac gtcagccgaa cggtagttcg tgaagccatc atcatgctgg aagttgaagg
      241 ctatgtggag gtgcgtaaag gttcaggtat tcatgtggtt tccaaccagc cgcgccatca
      301 gcaggcggct gacaataata tggaattcgc caattacggt ccgtttgagt tgcttcaggc
      361 tcgccagctc atcgaaagta atattgccga gttcgcggca actcaggtaa cgaaacaaga
      421 catcatgaaa ctgatggcca ttcaggaaca ggcgcgcggc gaacaatgct tccgtgattc
      481 cgagtgggat ttacagttcc acattcaggt agccctggcg acgcagaact ccgccctggc
      541 ggccatcgtt gaaaaaatgt ggacccagcg tagccataac ccgtactgga aaaaactaca
      601 cgaacacatt gattcccgta ccgttgataa ctggtgtgat gaccacgatc aaattctcaa
      661 ggcgttaatt cgcaaagatc cccatgctgc taagctggca atgtggcagc atctggaaaa
      721 caccaaaatc atgttattta acgaaaccag cgacgatttc gagttcaatg ccgaccgcta
      781 tctattcgcc gaaaacccgg tagtgcatct cgatacggcc accagtggca gtaaatgaaa
      841 ctcctgtccg acaggcgctg cgacgcgcct gttcgcttcg tcggtaagcg aaaagtataa
      901 agtgtcagcc tgtgtaaatc ctctcgccac cctcccctgc attcagcaaa atcagactcc
      961 acggacatgt aattttgata acgaacaacg ttgacctttg ttacaattag attcaatttg
     1021 aatttatgtt tttgaatgct ttcttatctc acgatttaac agggaatagt tcaggctgtg
     1081 ttgatgtatc aaacccgcag aacataccaa aacagcaata acattgcggt agtgcatctt
     1141 ttaaaaccag cgtggcgtta accgattcac caggaataat gaatggaact tttgacccaa
     1201 ttgctgcaag ccctgtgggc gcaggatttt gaaaccctgg ccaatccatc gatgattggc
     1261 atgttgtatt ttgtcttgtt tgtaattttg ttccttgaaa acggcttgct tccagcggcc
     1321 tttttaccgg gcgacagttt actggtattg gtcggcgtgt tgattgcgaa aggcgcgatg
     1381 ggttacccgc aaacgattct gctgctgacc gttgccgcca gcctcggctg ctgggtcagc
     1441 tatattcagg ggcgatggct gggcaatacc cgcaccgtac aaaactggct atctcattta
     1501 cccgcgcatt atcatcaacg cgcacaccat ctttttcata aacacggttt atcggcgctg
     1561 ttaattggtc gctttattgc gtttgtcaga acactgctgc cgacgattgc cgggttatca
     1621 gggctgaata acgcgcgctt tcagtttttc aactggatga gcggtctgct gtgggtattg
     1681 atcctgacaa ctctgggtta catgctcggc aaaacgccga tatttttaaa gtacgaggac
     1741 cagctgatgt catgcctgat gctgctcccg gtggtgctgc tggtgtttgg cctggcaggt
     1801 tctctggtcg tgttatggaa aaagaaatat ggaaatcggg ggtaagggat gcaaatacct
     1861 cgcatgtcgc ttcgccagct agcctggtcc ggcactgttt tacttctggt cggcacgctg
     1921 ttgctggcct ggtccgcggt tcgccagcaa gagtctacgc tggcgattcg tgccgttcat
     1981 caaggcacaa cgatgccaga cggtttttcg atctggcatc accttgacgc tcatggcatt
     2041 cctttcaaaa gtatcacccc caaaaacgac actctgttaa ttacatttga ctccagcgac
     2101 cagagcgccg ccgcaaaagc ggtcctcgac agaacattgc cccatggcta catcattgcg
     2161 cagcaggaca ataacagtca ggctatgcag tggctgaccc ggttacggga taactctcat
     2221 cgcttcggat aacttcctgt tatccgaaac atatcactca ctttggtgat ttcaccgtaa
     2281 ctgtctatga ttaatgaggc ggtggttaac actaccgctt cgttgtctgg acaatcgttc
     2341 ctttgtaata ggtccgaatc acaatggaag gttcaagaat gaaataccgc atcgctttag
     2401 ctgtttctct ctttgctctt agtgccggta gttatgccac taccctgtgt caggaaaagg
     2461 agcaaaatat ccttaaggag atcagctatg ccgaaaaaca ccaaaaccag aatcgtattg
     2521 acggtctgaa taaagccctg agtgaagtcc gggccaactg ttcagatagc cagctgcgtg
     2581 ccgatcatca caagaaaatc gcaaagcaga aagatgaggt ggcggaacgc cagcaagatt
     2641 tagccgaggc gaagcaaaaa ggcgatgccg ataagattgc caaacgcgaa cggaaactgg
     2701 cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc acaatagtca
     2761 ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca tctgcgtgct
     2821 gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg cgagaagtcg
     2881 aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca gagccgttat
     2941 cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc ggcgcgtgct
     3001 gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc aatcggtgta
     3061 gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac gcgcaagggc
     3121 ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt gaaatggtgg
     3181 aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat ctctttcaac
     3241 ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg agcctgatgg
     3301 tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt gccaccaccg
     3361 tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa tcgcgtaaat
     3421 ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg ctcgaggagg
     3481 agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct tagccagatc
     3541 cagcaacaac ggctggatct ttccgccagt cgtcgtgaat ggctggaggc aacaggcgct
     3601 tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt tggcagtagc
     3661 gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc cagacgcggt
     3721 tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca acagcttcgc
     3781 ggttaacttc cctctggccg gagcaattcc ggccttatcc ctcaaatttt ttgaagattt
     3841 ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc tccatcgaca
     3901 gcaacgacgc taataccgcg cccttgcaca aaaaaacaat cagcagcctg agtggcgcag
     3961 tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcgc attttaatgc
     4021 cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt acccaacaat
     4081 atatggaagc aatgggtgtc ccgggtttta tgctgccgct ggtgattctg cttgagttcg
     4141 gtggtggtct ggcaatcctg ttcggtttcc tgactcgcac cacagccctg tttactgcgg
     4201 gctttacgct gctgacggca tttttatttc acagcaactt tgctgaaggc gtcaactcgc
     4261 tgatgttcat gaaaaacctg acaatttctg gcggattcct gctgctggca attaccggtc
     4321 cgggcgcgta tagcatcgac cgcctgctga ataaaaagtg gtaagcacgc gctatactta
     4381 acgataaaaa acgaggagga agctcctcgt ttttgctatt ggaggagaga aaaatgggtc
     4441 aactgattga cggcgtctgg catgacacct ggtacgatac caaatctacc ggcggtaaat
     4501 ttcaacgttc agcttccgca tttcgtaact ggctcactgc cgatggcgca cctggcccca
     4561 ctggcacagg cggttttatc gcagagaaag atcgttatca tctctatatt tcactcgcct
     4621 gcccgtgggc gcaccgcacg ctgatcatgc gcaaactcaa aggactggaa ccgtttattt
     4681 ccgtttccgt agtgaacccg ctgatgctgg aaaacggctg gacctttgat gacagttttc
     4741 cgggagcaac cggagatacg ctctatcaac atgaatttct gtatcagctt tatctccacg
     4801 ccgatccaca ctacagcgga cgagttactg ttcccgtact gtgggacaaa aagaaccaca
     4861 ccatcgtcag caacgaatca acagaaatca tccgcatgtt caataccgcg tttgatgcgc
     4921 tgggcgcgaa agcgggtgat tactacccac cagccctgca aacgaaaatt gacgaactta
     4981 acggctggat ttatgacacc gttaacaacg gcgtgtataa agctggtttt gccaccagcc
     5041 agcaagccta cgacgaggcg gtggcgaaag tgtttgaatc gctggcgcga ctggagcaga
     5101 ttttaggcca gcatcgttac ctgaccggca accagctaac cgaagccgat attcgcctgt
     5161 ggactacgct ggtgcgtttt gatccagtgt atgtgaccca cttcaagtgt gataagcacc
     5221 gcatcagcga ttacctgaat ctgtatggct tcctgcgcga tatctaccag atgccgggga
     5281 tcgccgaaac tgtcaatttc gatcacatcc gtaatcatta cttccgcagc cataagacca
     5341 tcaaccctac ggggattatt tcaattggtc cgtggcagga tctcgatgaa ccgcaggacg
     5401 agatgttcgc ttcggttaaa aaaaggcacc ttacgaagtg cctctgataa ttagctaagt
     5461 gcatgttgtt catatcggat gcggcgtgaa cgccttatca aacctacaaa acatttatat
     5521 tcaatatgtt gcaatgaccg cgcaggccag ataagcgtag cgcatctggc gattttgcgc
     5581 ttgtcatcag tcttagcgcg ccttcacagc actttattaa ttccttgaac gaatatttac
     5641 tgccatttca ttcacgtcta ttcttaattt gctgctttaa aaacaagtga ttgagcaaaa
     5701 ttgaggcaaa aatggactgg tatctgaaag taataaaaaa ttatgtcggt ttccggggtc
     5761 gtgcgcggcg caaagagtac tggatgttta ttctggtcaa catcatcttt acgttcgtgc
     5821 tggggctgct ggataaaatg ttaggctggc aacgcgctgg tggcgaaggc atcctgacga
     5881 caatctacgg tattctggtg tttttaccgt ggtgggcagt tcagttccgc cgcctgcacg
     5941 acaccgaccg ctcggcgtgg tgggcactgc tattcttaat cccgtttatc ggctggctta
     6001 ttatcatcgt ctttaactgc caggcgggta cgccaggcga aaaccgcttt ggacctgatc
     6061 caaaactgga accttaaaac aaatctgctc gccggatgtc tgactcatcc ggcttttatc
//
LOCUS       DAZPBM010000213         6090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPBM010000213 DAZPBM010000000
VERSION     DAZPBM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.214"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(1..852)
                     /gene="melB"
                     /locus_tag="ACHUZQ_003859"
                     /pseudo
     CDS             complement(1..852)
                     /gene="melB"
                     /locus_tag="ACHUZQ_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
     gene            complement(955..2310)
                     /gene="melA"
                     /locus_tag="ACHUZQ_003860"
     CDS             complement(955..2310)
                     /gene="melA"
                     /locus_tag="ACHUZQ_003860"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1027447.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2593..3501
                     /gene="melR"
                     /locus_tag="ACHUZQ_003861"
     CDS             2593..3501
                     /gene="melR"
                     /locus_tag="ACHUZQ_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1027448.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3700..5967
                     /gene="adiA"
                     /locus_tag="ACHUZQ_003862"
     CDS             3700..5967
                     /gene="adiA"
                     /locus_tag="ACHUZQ_003862"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1027449.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1618 a         1609 c         1449 g         1414 t
ORIGIN      
        1 tactaacgtc accaggttag cagctcccgc atacgacaga taatagggga acaaatccgc
       61 atcaccgata acatatgaga aataatagat agcaaatccg gtaataatgt tgctggctac
      121 attataagca agagccatac ccaagaggca tgaaagctga tcgtttttat aaattagcgc
      181 aacgatggct ttaagtgtca gatggcttcc ttcagcagac ggttgattgt ctgacgaaaa
      241 gacttcatgc acattgcgca gagtgatgat ggttgaaaca ataaaaaagg cgatcagtac
      301 cagtgtgaac atctgaaagc caaatccccg atcgccaccg ccgacataat taacaaatgg
      361 tagcgtcaca cctgccgtaa caaagcccgc cagactggca aaaaaacgcg gataaggaac
      421 cagttgttca cgctcacgtt tatcgagcgt gatggttgga accagcgacc agaagggaat
      481 atccataatg gtgtaagtca tgccccagag gatgtaggtc acgcaaacaa agacaatctg
      541 agtagtacct tcaaacagat gcgcactaaa gaggagaaat aagattacag agtttgccaa
      601 cgtaccgatc aggatccagg gtttgaactt acctcatcgc gatcgcgtag catttacaat
      661 ccatcccata atcggatcgt taatagcatc ccagatcctc gccaccagaa acaaagtacc
      721 gaccaaaccc acagacagcc caacgacatc ggtgtagtaa tacatgaggt acatatacac
      781 aatgccgatc gcaaaatcct tcccgaacgc tccaaatcca taactgagtt ttgtagtcat
      841 tgaaatgctc atagggtatc gggtcgctgt taccaacgcc cgccttctgt tattggtatc
      901 gtcagaggtg tgcgccggaa taaatccccc ggcgcagtag cgtttagtcg cgttttaacg
      961 gtgcaaccag cctggcagcc agtcgccgtg ggcggcaatc aggtcgtcaa caagagcata
     1021 tatttcgtca atgcccagca cggcggcagt atgcgggtcc atcatcgcgg cgtggtaaac
     1081 acggtcgcga ttttccgtaa gaatagcttc ggtcagcagc gtctgtacat tgatgttggt
     1141 ttgcatcagg gcggccagat gcgaaggtag cgtaccgact ttagtcggct gaatgccatt
     1201 agcatcaacc agacaggcta cttccacgca acatccttgt ggcaggttat caatcaaacc
     1261 atcgttacgg acgttgccgt aaatcacact cggctcgcca gtccagatag cgttcatgat
     1321 tgtgctggca tattctcgtg acggtttaat atcaatccgg gaggcgtttt tatactcctc
     1381 cagctcttta tgccagttcg ccagctgctc gacgcagcgt ttcgggtact catccagcgg
     1441 tactttataa cgctcaatca aatcctcacg acctggctta ataaaccacg gcgtgtattc
     1501 agcaaaatgt tctgacgatt ccgtaacgaa gtagcccagc tttttgaaca tttcgtagcg
     1561 cacaatattc tggcagcgag tattaccatg aatatttggc ttcggtgcct gccctgcttc
     1621 ataagccgcc agcagttccg ggtagagatt cacgtaactg ccgtcggcgg ttttacgctc
     1681 cagttccagg taaaacgcca tatggttgat accagcgcaa cggtaacgca gcgtggacgg
     1741 atcgatattg aggtcacgcg ccagctcttc cgccgttccc tgcaccgaat ggcacagccc
     1801 tacctgtttg atatgcggat agcgggcata catcgcccag gtattcatcg ccattgggtt
     1861 aacgtagttg agcatggtgg catcggggca gacttccgtc atgtcctcgc aaatttgcca
     1921 cagatgcgga atggtacgta gcgcgcgcat aataccgccc ggccccaacg tatcggcaat
     1981 ggtttgttcc agaccatgcc gcttacagac ctcgaaatca gtcaccgtgc aaggttcata
     2041 accgccaatc tgaaatgcca ccacgacaaa atcggcatcc tgtaaggctt ctttctgttg
     2101 ggtgtggcag gtgattttgc cgctggcccc tgctgaatcc atcagcttac gcaccacaat
     2161 atgcgactct tccaggcggg tgggatcaat gtccatcagg gcaatatgcg ccgttttcag
     2221 cgcctcgcga tggaacacat caccaagaat atttttaacg aaaatcgtcg aaccagcgcc
     2281 gataaatgta attttgggtg cagtcatcat gcagatctcc tggcttgctt gaataacttc
     2341 atcatggcag gcgaatctgc agaaaacctc cggcttccag gttgagcatt cccataaact
     2401 cagatcctgc gtgaagcagc agtaaatctg agtttatggt aataagttac ctgaaaagca
     2461 gagttttcgg cctcatgatg ctcggacgct cccctcgcct ctctgggaag agggtgaaaa
     2521 ccgtgctccc actcgcagtc atcctccctc actcctgcca taattctgat attccaggaa
     2581 agagagccat ccatgaatac agatacgttt atgtgcagca gcgacgaaaa acagacccgc
     2641 agcccgctgt cgctgtattc ggaataccag cggatggaga ttgagtttcg cgcaccgcat
     2701 atcatgccca ccagccactg gcatggtcaa gtcgaagtga atgtgccttt cgatggcgat
     2761 gtggaatacc tgatcaacaa tgaaaaagtg aatatcaatc agggtaatat cacgctgttc
     2821 tgggcctgta caccgcacca actaacagat accggaacct gtcagagcat ggcgattttt
     2881 aatctgccga tgcatctgtt tctctcctgg ccgctggata aagacctgat taaccacgtc
     2941 actcacggca tggtgatcaa atcactggcc acacagcaac ttagcccgtt tgaagtgcgc
     3001 cgctggcagc aggaattaaa cagtccgaac gagcaaattc gccagctcgc cattgatgaa
     3061 attggcctga tgctcaagcg atttagcctc tctggctggg aaccgattct ggtcaataaa
     3121 acctcgcgca cacacaaaaa cagcgtctcg cgccatgcgc aattttatgt tagccagatg
     3181 ctgggcttta ttgccgaaaa ctatgatcag gcgctgacca tcaacgatgt ggctgagcac
     3241 gtcaaactta acgccaacta tgcaatgggg atattccagc gggtcatgca attgacgatg
     3301 aaacagtaca ttaccgcgat gcgcatcaac cacgttcgcg cgttactgag cgataccgat
     3361 aaaagtattc tcgatattgc cctgacggca ggctttcgtt caagtagtcg tttttacagc
     3421 acgttcggca aatatgtcgg catgtcgccg caacaatacc gcaaacttag ccaacagcgc
     3481 cgccagacgt ttcccggcta aagacgatat cagtatcagc caaaaaaata gtttagccgt
     3541 cgataataag cagtgaaaat aatttaaaat aatcacataa cgcattgttt ataaaaataa
     3601 atatttcaaa aatgtttgtt tttcacgcgc tttacagccc gaaaaggccg gaagatactt
     3661 gcccgcaacg aagattcctt cataaccggg taagcaatga tgaaagtatt aattgttgaa
     3721 agcgagtttc tccatcaaga cacctgggtc ggtaacgccg ttgagcgtct ggcagatgct
     3781 ttaagccagc aaaatgttac cgtgattaaa tccacctcct ttgatgatgg ttttgccatt
     3841 ctctcttcaa acgaagccat tgactgcctg atgttcagct atcaaatgga acatccggac
     3901 gaacatcaaa acgtcagaca attgatcggt aagcttcatg agcgccaaca aaacgtgccg
     3961 gtcttcctgt tgggcgatcg ggaaaaagcc ctcgccgcaa tggatcgcga cctgctggag
     4021 cttgtcgatg aattcgcctg gattctggaa gataccgccg actttatcgc cggacgcgct
     4081 gttgccgcga tgacccgcta ccgccagcag ctgttgccgc cactgttcag cgcgctgatg
     4141 aaatatagcg acatccatga atattcctgg gcagcgccag gccaccaggg cggcgttggt
     4201 tttaccaaaa cacccgccgg acgtttctac catgactact atggtgaaaa tctgttccgc
     4261 accgatatgg gcatcgaacg aacttccctc ggttctttgc ttgaccatac tggcgcattt
     4321 ggcgaaagcg aaaaatatgc cgcacgcgta tttggtgccg atcgctcctg gtcggtagtc
     4381 gtcggtactt ccggctctaa ccgcaccatc atgcaggctt gcatgaccga taacgatgtc
     4441 gtggtcgttg accgtaactg ccataaatcc atcgaacaag gcttgatgct gacaggcgcg
     4501 aaaccggtct acatggtgcc aagccgcaac cgctacggca ttatcgggcc aatctatccg
     4561 caggaaatgc aacctgaaac cttgcagaag aaaatcagtg aaagcccgct gactaaagac
     4621 aaagccgggc aaaaaccgtc ttactgcgtg gtgaccaact gcacctatga cggcgtgtgt
     4681 tataacgcta aagaagcgca ggatctgctg gaaaaaacct ccgatcgtct gcactttgac
     4741 gaagcctggt acggctatgc acgtttcaac ccgatctatg ccgatcacta tgccatgcgc
     4801 ggcgaacctg gcgatcacaa cggtcctacc gttttcgcca cccactccac ccacaaactg
     4861 ctgaatgcgc tttcacaggc ttcttatatt catgtacgtg aaggtcgtgg ggcgattaac
     4921 ttctcccgct tcaaccaggc ctacatgatg catgccacca cctccccgct gtatgccatc
     4981 tgcgcatcca acgacgtggc ggtgtcgatg atggacggca acagcggcct gtcactgacg
     5041 caggaagtga tcgacgaagc ggttgatttc cgtcaggcga tggcgcggct gtataaagag
     5101 ttcaccgctg acggtagctg gttcttcaaa ccgtggaaca aagaagtcgt caccgaccca
     5161 caaaccggca aaacctatga ctttgctgac gcaccaacca aactgctgac caccgttcag
     5221 gactgctggg taatgcatcc gggcgaaagc tggcacggct tcaaagatat tccggataac
     5281 tggagtatgc tcgacccgat taaagtcagc atccttgctc cgggaatggg tgaagatggt
     5341 gaactggaag aaaccggtgt tccggcggcg ctggtcactg cctggcttgg tcgccacggc
     5401 attgtgccta cccgcaccac tgacttccaa attatgttcc tgttctctat gggcgtaacc
     5461 cgtgggaaat ggggaactct ggttaacacc ctttgctcct tcaaacgcca ctatgacgcc
     5521 aacacaccgc tggcgcaggt gatgccggaa cttgttgaac aatatcctga cacttacgcg
     5581 aacatgggga ttcacgatct gggtgacacc atgtttgcct ggctgaaaga aaacaaccct
     5641 ggcgcacggt tgaacgaagc ctattccggc ctgtcggtgg cggaaatcac cccgcgtgaa
     5701 gcgtacaacg cgattgtcga caacaatgtc gaactggtat ccattgaaaa tctgccagga
     5761 cgcatcgcgg caaactcagt tatcccatat ccgccaggaa tcccgatgct gctgtctggt
     5821 gaaaacttcg gcgataaaaa cagtccgcaa gtaagttatt tacgctcgct gcaatcctgg
     5881 gaccaccatt tccctagatt tgaacacgaa actgaaggga ctgaaattat tgacggtatt
     5941 taccacgtta tgtgtgtgaa agcgtaacca ctattccgct gaaggcgtaa ttgtttaaat
     6001 aacattacgc cgcctggcct taggcctttt gagtatggca acgttttcat aaaaattgct
     6061 gcaaacaaaa atgtcatact ttttgcgcgg
//
LOCUS       DAZPBM010000214         6026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPBM010000214 DAZPBM010000000
VERSION     DAZPBM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.216"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            167..517
                     /gene="traA"
                     /locus_tag="ACHUZQ_003863"
                     /pseudo
     CDS             167..517
                     /gene="traA"
                     /locus_tag="ACHUZQ_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            532..843
                     /gene="traL"
                     /locus_tag="ACHUZQ_003864"
     CDS             532..843
                     /gene="traL"
                     /locus_tag="ACHUZQ_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR1027450.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            865..1047
                     /locus_tag="ACHUZQ_003865"
                     /pseudo
     CDS             865..1047
                     /locus_tag="ACHUZQ_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            1049..2299
                     /locus_tag="ACHUZQ_003866"
                     /pseudo
     CDS             1049..2299
                     /locus_tag="ACHUZQ_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2296..2682
                     /gene="trbI"
                     /locus_tag="ACHUZQ_003867"
     CDS             2296..2682
                     /gene="trbI"
                     /locus_tag="ACHUZQ_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR1027451.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2679..2990
                     /locus_tag="ACHUZQ_003868"
                     /pseudo
     CDS             2679..2990
                     /locus_tag="ACHUZQ_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            2985..3983
                     /locus_tag="ACHUZQ_003869"
                     /pseudo
     CDS             2985..3983
                     /locus_tag="ACHUZQ_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            4003..4750
                     /gene="traX"
                     /locus_tag="ACHUZQ_003870"
                     /pseudo
     CDS             4003..4750
                     /gene="traX"
                     /locus_tag="ACHUZQ_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4805..5365
                     /locus_tag="ACHUZQ_003871"
     CDS             4805..5365
                     /locus_tag="ACHUZQ_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1027452.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5495..5707
                     /locus_tag="ACHUZQ_003872"
     CDS             5495..5707
                     /locus_tag="ACHUZQ_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1027453.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1426 a         1461 c         1836 g         1303 t
ORIGIN      
        1 actcacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc
       61 tccggcagaa gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg
      121 agttcacaga taaaacactc tccaggaaac ccggggcggt tcagcgttaa gtgttcaggg
      181 tacttctgcg cccgtcaaaa agaaatcgtt tttttctaaa ttcactcgtc tgaatatgct
      241 tcgcctggtt cgtgcagtga tcccggttgc tgttctgatg atgttgttcc ctgagttagc
      301 tatggctgct ggtaaaggca cggacctgat ggctaaaggt caggaaacgg ttaaggcgac
      361 cttcggtaag gactccagta tcgttaaatg ggttgttctg gctgaagttc tggtcggtgc
      421 agtcatgtac atgatgacca aaaacgtcaa gttcctggtc ggctttgcca tactctctgt
      481 atttattgca gtaggtatgt ctgttgccgg attctgacag gaaataaaac gatgttggga
      541 gacgagaata aacttaagaa atatcgtttc ccggaaacac tgaccaacca gagccgctgg
      601 tttggcctgc cactggatga actgatcccc gcagcaatct gtgttggctg gggtatcaca
      661 acatcgaaat atctgttcgg tattggtgca gcggttctgg tttatttcgg gattaaaaaa
      721 ctgaaaaaag ggcggggcag ttcctggtta cgtgacctga tttactggta tatgccaaca
      781 gccctgctgc gcggtatttt tcataatgtt cccgattcgt gtttccggca gtggattaaa
      841 tagaactgat accaggattg ttatatggaa cacggtgccc gtttaagtac cagtcgtgta
      901 atggccatcg cctttatatt tatgtcagtg cttattgttc tcagcctctc tgttaacctc
      961 attcaggggg tgaataacta ccgtcttcag aatgagcaac gcactgccgt gaccccaatg
     1021 ggatttaatg tgccctttgc cgtgtcacac ctaccgtaac cagctggcgt ttatcgatat
     1081 tttcttccgg ggaatgaata acaccaacta caacatggcg gtctgtggca cctccggggc
     1141 cggtaaaacc gggctgatac agccacttat ccgcagcgtg ctggactccg ggggctttgc
     1201 cgtggtgttc gacatggggg atggctacaa gtccctgtgt gagaacatgg ggggcgtata
     1261 tctggacggt gaaaccctgc gttttaaccc gtttgcgaac atcaccgata tcgaccagtc
     1321 agcggagcgt gtccgtgacc agttgtcagt gatggccagc cccaacggta acctggatga
     1381 agtgcatgaa ggtctgctgc tgcaggcggt cagggcctcc tggctggcca aagagaacag
     1441 ggcacgtatt gatgacgtgg tggatttcct gaaaaacgcc agtgacagcg agcagtatgc
     1501 cgggtcaccg actatccgca gccgtctgga cgaaatgatt gtgctgcttg accagtacac
     1561 tgccaacggc acttacggcc agtattttaa ctctgatgag ccgtccctgc gggatgacgc
     1621 aaaaatggtg gtgctggagc tgggcggact ggaagaccgt ccgtcactgc tggttgcggt
     1681 gatgttctcc ctgattatct acatcgagaa caggatgtac cgcacgccgc gtaacctcaa
     1741 gaaactgaac gtcattgatg aaggctggcg tctgctggac ttcaaaaatc acaaggtcgg
     1801 tgagtttatt gagaaaggtt accgtaccgc ccgtcgtcat accggtgctt atatcaccat
     1861 cacacagaac atcgtcgact ttgactctga caaggcctcc agtgcggccc gtgcggcatg
     1921 gggtaactcc tcctacaaaa ttatcctcaa acagagtgcg aaggagttcg caaaatacaa
     1981 ccagctgtat ccggaccagt tccagccact gcagcgcgac atgattggta agtttggtgc
     2041 ggccaaagac cagtggttca gttccttcct gctgcaggtg gaaaaccatt cctcctggca
     2101 ccgcctgttt gtggacccgt taagccgcgc catgtacagc tctgacggcc cggattttga
     2161 gtttgtgcag cagaaacgta aagaggggct gagtattcat gaggcggtgt ggcagctggc
     2221 gtggaagaag tcagggccgg agatggcttc gctggaagcc tggctggaag aacatgagaa
     2281 atacaggagt gttgcatgac cacaacgcaa aaaacgactg atgttacagc cccccgacgc
     2341 agccactggt ggtggacggt gccgggatgc ctggcaatgg tgttactgaa cgcagccgtc
     2401 agttacggca ttgtcagact gaacgcaccg atgactgccg ccttcaacat gaaacagact
     2461 gtggatgcat tttttgacag cgccagtcag aaacaactgt cagaagcaca gtcaaaagcc
     2521 ctttcagcgc gttttaacac ggcactggaa gccagcctgc aggcgtggca gcagaaacac
     2581 cacgcggtca ttctggtgtc gcctgccgtg gtacaggggg ctccggatat cacccgggaa
     2641 atccagcagg atattgcccg gcgaatgaga gcggaaccat gagatgccgg gggctgattg
     2701 ccctgctgat atgggggcag agtgtggccg ccgccgatct tggcacctgg ggtgatctgt
     2761 ggccggtaaa ggagccggac atgctgacgg tgatcatgca gcgcctgacg gcactggagc
     2821 agtccggtga gatgggccgg aaaatggatg cctttaagga gcgggtgatc cgcaacagtc
     2881 tgcggccccc tgccgtgccc ggtatcggac gcacggagaa atacagcagc cggttgtttg
     2941 acccgtccgt cagactggct gcggatatcc gggataacga agggcgggtg gccggctttg
     3001 agtcagccta cgtggccctg tcgcgtatga agcagcatgt gcaggtgtac accgataacc
     3061 gtcagggctg gacggatgcc atcaacaatg ccgtacagaa aggaactgcc cacgatgtgt
     3121 ttgagccgaa accggaccgg gaggtcatga atgcagagcg gctgttcagt acggcgcggg
     3181 agctgcggga cgtggcggca ggccgtgccg ttctccgtca ggcggggctg gccgggggag
     3241 acagtcctgc acggtttatt gctccgggac gtaaatatcc gcaaccgtat gtggcactgc
     3301 cggcgtttga ccgtaacggc aagtccgccg gtatctggct gaacccgctg accacggatg
     3361 acggaaacgg gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc ggggatgcgc
     3421 agttcgtggc cctgcagggc agccgtaacg gagaaagcct gctggctgat aatatgcagg
     3481 atggcgtccg gattgcccgt gataatcctg acagtggtgt ggtggtaagc attgccggtg
     3541 aaggccgtcc gtggaatccc ggtgccatca ccgatggccg cgtgtggggg gatatcccgg
     3601 acaacagcgt ccagccggga gccggaaatg gcgaaccggt cacggcagag gtactggcac
     3661 agcggcaggc tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat gaaattgtcc
     3721 gtaaaatggc agagaacaaa cctgacctgc cggacggcaa aacagagctg gctgtcaggg
     3781 aaattgccgg gcaggagcgt gaccgggctg ccataactga acgggaagcc gcgctgccgg
     3841 agagtgtgct gcgtgaatca caacgggagc gggaagcggt ccgggaggtt gcccgggaaa
     3901 atctgctgca ggagcgtctg cagcagatgg agcgggatat ggttcgtgac ctgcagaaag
     3961 agaaaacccc gggcggagac tgatacagga agacaaacac tgatgacaac ggataacacg
     4021 aacacgacac gtaacgattc actggctgca cggaccgata cctggttgca gtcatttctg
     4081 gtctggtcac ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg
     4141 gaccacatca atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga
     4201 gcctttccgc tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg
     4261 caaccggcca ttaaccggct gtggggatgg ggaattattg cccagttcgc atattatctg
     4321 gcaggctttc cctggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg
     4381 ctgacgtggt gtgaaacgcg cagtgggtgg cgtacagcag cggccattct tctgatggcc
     4441 ctgttggggg cctttgtccg gcaccagtta cggcattgcc gggctgctga tgctggcagt
     4501 cagccaccga ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct
     4561 gctggctgtt attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct
     4621 tgtgatgacg gtgctgaccg ttggtctggt gtcgtgtgca ggaaaatcat taccccgttt
     4681 ctggcccggg gattttttcc cggtattcta cgcctgtcat ctggctgtgc tgggcgttct
     4741 ggcgctgtga cgggtgtggt atctttgacc gtaacaggat gattcgtcag aggcagaaca
     4801 cagcatgaca gagcagaagc gaccggtact gacactgaag cggaaaacgg aaggggagac
     4861 gcctgtccgc agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa
     4921 aaagcagaag ctggccgaga aagcagcccg ggaagcagag ctggcggcaa aaaaagcgca
     4981 ggccagacag gcactgtcca tttatctgaa cctgcccacg ctggatgagg ccgtgaatac
     5041 cctgaaaccc tggtggccgg gattatttga cggtgacacc ccccggcttc tggcctgcgg
     5101 tatccgggac gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa
     5161 actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc
     5221 cggtgcctgc cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga
     5281 agcgtatgcg gcagcgcgtc tggataaaat ccgccgccag aaccggataa aggcagaact
     5341 tcaggccgtg cttgatgaga aataaaaaaa agcctccatc agagaggagg cagggaaata
     5401 aggcttaaaa ggaaataact tccgcttccg aaaggaagcc agtccactga attatttatc
     5461 acaataatac tgacaactaa agggggacaa gaaaatgaat ggattcagaa acagttcacg
     5521 gaacggccag gtctggcgtt accagcgtgc cggcgggcgg gccgttattc tggaagtcag
     5581 tggacgctgg atggaagcgg cagaagcatg gcgacgggct gcctgtgttg cgcccaggac
     5641 agactggcag cagtttgccc gaaaaagggc tgaacactgc caccggcgct gccggggaag
     5701 ggtgtaacga aaaaagcagc cttcattact ccaggagtca gaaggctgaa aggagtttat
     5761 gagatttgct ttttttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca
     5821 gagttttttc cgtattaagt cgtgatttta tattttgtga ttaatttcac aaaataaggt
     5881 gttattcagt gtgtgctgca atattcagga tgcctgaaat acagatgcta tttatctgat
     5941 atggagaata acatgaggaa atagtaaggc agtatgactt tccgtgagac agatgctggc
     6001 caacgtggga tatttaacgc gggtta
//
LOCUS       DAZPBM010000215         6025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPBM010000215 DAZPBM010000000
VERSION     DAZPBM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.217"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(127..276)
                     /locus_tag="ACHUZQ_003873"
     CDS             complement(127..276)
                     /locus_tag="ACHUZQ_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027454.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            847..1005
                     /locus_tag="ACHUZQ_003874"
     CDS             847..1005
                     /locus_tag="ACHUZQ_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1027455.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="ACHUZQ_003875"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="ACHUZQ_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1027456.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="ACHUZQ_003876"
     CDS             2367..2669
                     /locus_tag="ACHUZQ_003876"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027457.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="ACHUZQ_003877"
     CDS             complement(2845..4338)
                     /locus_tag="ACHUZQ_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFR1027458.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="ACHUZQ_003878"
     CDS             complement(4382..4771)
                     /locus_tag="ACHUZQ_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027459.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="ACHUZQ_003879"
     CDS             4989..5429
                     /locus_tag="ACHUZQ_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR1027460.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1428 a         1663 c         1581 g         1353 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctggaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 tgaactgaag gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg
     5881 aacgatacag tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc
     5941 cgccatggtt gattattttt caacctcaaa atggtcatac acctaacgca gccgcgattt
     6001 tcatcgcaac tgcatgaatt aaaag
//
LOCUS       DAZPBM010000216         5931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPBM010000216 DAZPBM010000000
VERSION     DAZPBM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.218"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHUZQ_003880"
     tRNA            292..368
                     /locus_tag="ACHUZQ_003880"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUZQ_003881"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUZQ_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1027461.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHUZQ_003882"
     CDS             complement(1357..2247)
                     /locus_tag="ACHUZQ_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1027462.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUZQ_003883"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUZQ_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR1027463.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHUZQ_003884"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHUZQ_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR1027464.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1561 c         1549 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAZPBM010000217         5877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPBM010000217 DAZPBM010000000
VERSION     DAZPBM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.219"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(39..401)
                     /locus_tag="ACHUZQ_003885"
     CDS             complement(39..401)
                     /locus_tag="ACHUZQ_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1027465.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="ACHUZQ_003886"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="ACHUZQ_003886"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1027466.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHUZQ_003887"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="ACHUZQ_003887"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1027467.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHUZQ_003888"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="ACHUZQ_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1027468.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHUZQ_003889"
     CDS             complement(4828..5037)
                     /gene="yibT"
                     /locus_tag="ACHUZQ_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1027469.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="ACHUZQ_003890"
     CDS             5322..5684
                     /locus_tag="ACHUZQ_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1027470.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1490 a         1451 c         1520 g         1416 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgata
     5821 ttctgaaaat gcatttttac cgaaatagcc acctataaac aataaaaata tatagaa
//
LOCUS       DAZPBM010000218         5865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPBM010000218 DAZPBM010000000
VERSION     DAZPBM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.220"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..560)
                     /locus_tag="ACHUZQ_003891"
     CDS             complement(<1..560)
                     /locus_tag="ACHUZQ_003891"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR1027471.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
     gene            complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHUZQ_003892"
     CDS             complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHUZQ_003892"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1027472.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHUZQ_003893"
     CDS             complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHUZQ_003893"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1027473.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2371..3636)
                     /locus_tag="ACHUZQ_003894"
     CDS             complement(2371..3636)
                     /locus_tag="ACHUZQ_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1027474.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3863..5077)
                     /locus_tag="ACHUZQ_003895"
     CDS             complement(3863..5077)
                     /locus_tag="ACHUZQ_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1027475.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5177..5533)
                     /locus_tag="ACHUZQ_003896"
     CDS             complement(5177..5533)
                     /locus_tag="ACHUZQ_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1027476.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5669..5776)
                     /gene="rprA"
                     /locus_tag="ACHUZQ_003897"
     ncRNA           complement(5669..5776)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUZQ_003897"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1330 g         1506 t
ORIGIN      
        1 cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac
       61 tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc ttgctggcgt
      121 ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg
      181 gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt
      241 tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc ccatttcacc
      301 agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag ccctgtcgga
      361 ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc aagagctgta
      421 attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg
      481 tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga
      541 ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc ctacgccaga
      601 tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt aaatatattt
      661 tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
      721 tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag gcttttttta
      781 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
      841 tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc atctccaggg
      901 tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
      961 tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
     1021 ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga
     1081 cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata tcaaccaggc
     1141 cgctgtcgat ggctgcacga ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc
     1201 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtttcacgga
     1261 gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag tggtccacac
     1321 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgcggg
     1381 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgcacca atgacgagat
     1441 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
     1501 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataacgtga
     1561 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caacctgctt
     1621 gttgtgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagca
     1681 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg gggttcatac
     1741 ctacttttgt gccattggtt aaaatgtcgg cggaagccag gacttcagca aaggcttgct
     1801 gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga
     1861 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
     1921 cttcaattgc cccctgcgcg ccaatcgctg ttgaggcacc tccggcccct aacagcacca
     1981 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
     2041 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcacccacca
     2101 gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
     2161 acacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg ggaaagctat
     2221 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
     2281 tctgcatttc aggcgataaa ctgtggcgga taggataagc catcaaccca atcaattcgt
     2341 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
     2401 ggtgctaaat ttgcgttcgc caaaccgcac atcattctca ggaataataa aaacgcggta
     2461 atagcggcta aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
     2521 aacaatgata tattgcaggc cgatgttcga cagataaccg gtaatcagcg gaataacaaa
     2581 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttggggaa
     2641 aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg agaaaccgat
     2701 aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa tggcagctgt
     2761 tattgttgcc agcgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
     2821 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
     2881 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
     2941 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
     3001 ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg cgtccaccaa
     3061 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca gaatacccgg
     3121 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
     3181 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccagaggc
     3241 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
     3301 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
     3361 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
     3421 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
     3481 ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgtca tattttgggc
     3541 aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa gaacagccag
     3601 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
     3661 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     3721 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3781 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3841 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3901 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3961 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     4021 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
     4081 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     4141 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     4201 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     4261 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     4321 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     4381 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     4441 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     4501 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     4561 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     4621 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     4681 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     4741 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     4801 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     4861 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     4921 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4981 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     5041 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     5101 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     5161 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     5221 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     5281 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     5341 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     5401 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     5461 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     5521 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     5581 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     5641 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     5701 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5761 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5821 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctacc
//
LOCUS       DAZPBM010000219         5720 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPBM010000219 DAZPBM010000000
VERSION     DAZPBM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.221"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(16..285)
                     /locus_tag="ACHUZQ_003898"
     CDS             complement(16..285)
                     /locus_tag="ACHUZQ_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1027477.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            342..1010
                     /locus_tag="ACHUZQ_003899"
     CDS             342..1010
                     /locus_tag="ACHUZQ_003899"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1027478.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSA
                     SGKK"
     gene            complement(955..1092)
                     /locus_tag="ACHUZQ_003900"
     CDS             complement(955..1092)
                     /locus_tag="ACHUZQ_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR1027479.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1065..1163)
                     /locus_tag="ACHUZQ_003901"
                     /pseudo
     CDS             complement(1065..1163)
                     /locus_tag="ACHUZQ_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1152..1391)
                     /locus_tag="ACHUZQ_003902"
                     /pseudo
     CDS             complement(1152..1391)
                     /locus_tag="ACHUZQ_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1490..2380
                     /locus_tag="ACHUZQ_003903"
                     /pseudo
     CDS             1490..2380
                     /locus_tag="ACHUZQ_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2383..2916
                     /locus_tag="ACHUZQ_003904"
     CDS             2383..2916
                     /locus_tag="ACHUZQ_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1027480.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2945..3472)
                     /locus_tag="ACHUZQ_003905"
     CDS             complement(2945..3472)
                     /locus_tag="ACHUZQ_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1027481.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(3474..>5720)
                     /locus_tag="ACHUZQ_003906"
     CDS             complement(3474..>5720)
                     /locus_tag="ACHUZQ_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1027482.1"
                     /translation="TKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSA
                     STATTKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETN
                     AKSSETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTAT
                     TKAGKATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASAS
                     KDEATRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIAS
                     AVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPT
                     TPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNAL
                     AGKQPKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLG
                     LQETVNKAGNAVQRSGDKMTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQ
                     GENGNIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQ
                     DGDGIISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMF
                     LWKDNGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWL
                     HNHVVRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGG
                     IWYNVGSV"
BASE COUNT         1438 a         1371 c         1301 g         1610 t
ORIGIN      
        1 gcataatccc aatgattact ccctgacagg atttgcaggc cactcaatat caggtgcagt
       61 tgatgtatca acacgattca acaatacccg atatttattc catgcctcca gcaacgatct
      121 ttcttcctcc gttgcgattt ccagatctac agcatcctgc agtggcgcaa tatactcact
      181 aaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat
      241 cgaagcatac caggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
      301 tcatcgccat aattgattta attcacaaat aaaactataa catggtgaaa tcaatgaaaa
      361 caaacacaga tgatggggct aaaatttaca caccacttac cctaaagctt tatgactggt
      421 gggttttggg agtatcaaat cggcttgcat ggggatgtcc tacaaaggaa caccttcttc
      481 cacactttct ggaacattta ggtaacaacc atctggatat tggtgttgga actgggtttt
      541 accttactca cgtacctgag agtagtctga tatctttaat ggatttgaac gaagctagcc
      601 tgaacgcggc atctacaagg gctggggaat caaaaattaa acataaaatt agccatgatg
      661 tttttgaacc ttatcccgcg gcgttacatg gtcaatttga ttccatttcc atgttttacc
      721 ttcttcactg cctgcctgga aatatatcta caaaaagctg tgtaatacgc aatgcggcgc
      781 aggccttaac tgacgatgga actctatacg gagccacaat tcttggcgat ggagttgtgc
      841 acaatagctt cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc ttttcaaaca
      901 caaaagattc cgaagaaggc ttaacacata tactctcaga gcatttcgag aatgttaaaa
      961 ccagggttca aggtactgta gtaatgtttt ccgcttcagg gaaaaaatag catccaaccg
     1021 cagcacgttc ttgcttaaga cgtgctgcgg cataatccca atgattactc cctgacaggg
     1081 ttcgtaggcc actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga
     1141 tactttttct aggcttccac caccagcacg acaagatgcc gcatacagtg acccagtcag
     1201 tccagttttc agacaaccag tgcgtcacct ttttgaaggc gctttaaagc acgttttaat
     1261 ccaggtcggc ctgtccttgt tccgcttaat ttatcttcaa atatttgttc atatcctgca
     1321 caaacaagag cgtttcgttg caggtctgta ttctggtcat ttgttgatac ccttacatag
     1381 ccaatcagca cgctgaatct cccgtccaaa agcacaaatc atgccatgca ggccagaaac
     1441 cgccattatc taaaacctcg gtttacgaga actcggcaca agcggggaga aaataccgtt
     1501 actcagtaca gcgaatacct ggactaatcg acaaacattc agcggtggcc tttctggtga
     1561 actatccggc aatgcttcta cagctgcaaa attaaaaact gccaggaaaa taagcaatgt
     1621 ggcttttgat ggttcctccg atatcacatt aaaagcaagt catgttggtg cgtttgcctt
     1681 agggaaaaca ggaagcaccg ttgcgaatga taaagcagtt ggatggaact ggagtagcgg
     1741 agcctataac gcaactatta gtggtgcatc aacgttaatt attcattttt atatgggaga
     1801 aggaagttgt cctgcagctc agtttcggat taattataaa aatggcggta ttttttatcg
     1861 ttcagcccgt gatggttatg gttttgaagc cgactggtcc gaattttaca ccaccagaaa
     1921 accttcagca ggagatgttg gtgcactgcc gttatctggt ggtcaactga atggtgcact
     1981 gggtatcgga acatccagtg ctcttggcgg taattcgatt gtattgggtg ataatgacac
     2041 gggctttaaa caaaaaggcg atggtaatct ggatgtttat gctaataacg tccatgttat
     2101 gcgctttgtc tccggaagca ttcaaagtaa taaaaccata aatattacgg gacgtgttaa
     2161 tccctcggat tacggtaact ttgattcccg ctatgtgaga gatgtcagac ttggcacacg
     2221 agttgtttag accatgaaga aaggcgtgat gtatgaaaaa tcaggccatg taatcacggg
     2281 gcttggtatt atcggtgaag tcgatggtga tgatccggca gtattcagac caatacaaaa
     2341 gttaattaac ggaacatggt ataacgtatc gcaggtataa tcatgcagca tttaaaaaac
     2401 attgtcgcag gtaatccaaa aaccgttgaa caataccagc taacaaagaa ttttgatgtt
     2461 atctggttat ggtccgaaga tggaaaaaat tggcatgagg aattaaaaaa ctttcaggag
     2521 gatacaataa aactggctta cacagttgag ggaataatta ttgctataga caaggatgta
     2581 tcagcaatta atccagaagg tttaagtgtc gttgagttgc ctgatattac agcaaatcgc
     2641 cgggctgata tttcgggcaa ctggatgttc aaagatggtg cggtgataaa gcgaacttat
     2701 accgaggaag agcaaaggca acaagcggaa aatgaaaagc agagcctgct gcagctcgtc
     2761 agggataaaa cccagctgtg ggactctcag ctacagctgg gcatcatttc cgatgagaat
     2821 aaacaaaaat taaccgagtg gatgctctat gcgcagaagg ttgaatccac agacacctcc
     2881 agcctgccag taacgtttcc cgaacaacca gaatgagaga aggcccgcta tcgggcctta
     2941 atttttattc aggcttttgt ggccattcag gatttgccgt atccacacgg ctgaccagaa
     3001 cactgtagcg ttcccatgct tccagtcgtg tgcgttcctc gtctgttgcc atattcagcc
     3061 tgacagcgcg ttccagcggc tggatgactg attcagcttc ggaaagcaat gcggcctttt
     3121 gtgattcggc ctgttgttgc agttcctgtt ctgtatattc cctctttact actttaccat
     3181 ccagaaacat ccattttccg ttattgtctg cgtgacgatt tgccgtaata tcaggaactt
     3241 caactatgct tagcccttca ggattaatag ttgaaacgtc tttactgaca gcaacgatga
     3301 tgttgttctc atcgtaagca atttttatgg tgtctggctg aaaattcttt tgttcctcat
     3361 accagttttt gccatcttcg caaaagagcc ataccacgcc cgctttttta gttagctggt
     3421 attgttccgg tgttttcgga ttcccggcct taatattttt caggtgcatc atcttaaaca
     3481 ctccccacgt tgtaccatat tcccccaatc aatttctgaa ttggccttct ggcaacagtg
     3541 tcgacaatat catccgcatt gctgttaatt gcggctgtta aaacataacc tgacgcatcg
     3601 ccaaagccgt agtcgcgcca tacgttttta tattcaatgc tgccaagacg aatatcccga
     3661 acgacatgat tatgcagcca tgtgcttaac cagttgtttt cccacactga accataaata
     3721 tcgccactag acgcaacttg cgcaccactt cccagcgcaa atcccccatc tgttttaaaa
     3781 atgaaatcac caccaccatc tgaaccgtta ttaatatgaa caccatcacc accgttatct
     3841 ttccacaaaa acatacgaat tcttccgtct tcgttggcga atgtaatgtg gtggcggtaa
     3901 ttacttttta atctaaaatt gctgccatta gattcaatat ctttataaag atgtaatcct
     3961 aactcatcaa tatgtccgat gcgcgaacca tttgaataga agctaataat accatctcca
     4021 tcttgcctaa atccggtgtc gttatctccc agaacaatgg agttttcacc cagtgcgtta
     4081 tcagtagctc caatagctag acctccttta attttggccc cgtggctgac agatatagca
     4141 cctgttctca gatttatagc gaatggcctt aatgggccga tattaccgtt ttcgccttgt
     4201 ccttcagccg tcggaatgaa atgaagaaaa tcttctgaac gacggaaaat tagaccgaag
     4261 gtatcattga aaattcgcag cgcattcacc gtgccaattt tcagttctcc ggtcatttta
     4321 tcgccggaac gctgaacggc gttaccagcc ttgtttaccg tttcctgtaa accgaggtat
     4381 tcgataacgg cagcaacggt cgatttcgca agaatatccc gcccgacttt tgtcagggtt
     4441 gccaggctgg cgacatcatt ccccgtaaaa tacggaaact tgcctgccgc agtagtaagt
     4501 ccggccagcg ccgtcagggt ggcatctttc ggttgcttac ccgcaagcgc gttagtcatg
     4561 gtggtcgcaa aattcgggtc gttgcccagc gccgcagcca gctcgttcag cgtgttcagt
     4621 gcatcaggtg acgaatctac aagggcggca atcgcagcca taacgaaagc cgtgcttgcg
     4681 atttgggtat tattcgttcc ctgtcgcgca gttggtgttg ttggcgttcc ggtcagtgca
     4741 ggactattta agggcgcttt cttgttcgtt tcacccatta ccgccttaac cgcttttggc
     4801 gttgccgcca gtgactcgga agtgctgttg gtcgcactgc tgagctgtac tatccccttt
     4861 ttcgtcgtgc tcgcatcctc cagcgccacg gcggatgcaa tatcctctgc ccgttttgct
     4921 gctgtctcgg cgcgcgttgc cgcggattca gcagcaactt tgctctgaga tgcagccgtc
     4981 gcactgcctg ccgcctctga tgctttcgtt gttgctgtcg tggcactacc tttcgctgct
     5041 gacgcttgtc tggtcgcctc atcttttgaa gcagacgcag atgatgccga tgacgccgct
     5101 gaactggctg acgatgcggc tgctgcctta gaggaagcag cattgtctgc tgaagtcttt
     5161 gcatttgttt cagaggtttt tgctgcagaa gcagacctcg ctgctgcagt ggcttgctca
     5221 gtggctttgc cagccttcgt tgtggctgtt gaagcggatg atgcggcgct ttctgccgat
     5281 tttccggcgg cggtggcact ggctgaggcc tgcccggcac ttgttgacgc ggcactggca
     5341 gataatgcag ccgctgtttt tgaacctgcc gcagctgagg cgctctgtcc cgctgctgtt
     5401 tcagaagact tagcgttcgt ctcggacgtt tttgccgcct tcgcggaatt tcctgccgcc
     5461 gttgccgagg aagctgcact actggcactt gatgatgcat tcgtttctga agatttcgct
     5521 gcctcttttg aggccgccgc accccgtgcc gaggtggcag cttctgacgc cttcgtggtc
     5581 gctgtggatg cagaagtggc tgccgatttt tgtgatgctg cggcattcgt ttctgacgtt
     5641 ttcgcggcac tggcgctggt agctgccgcg ctttttgatg actctgcagc agcacttttt
     5701 gctgcttcac gggcctttgt
//
LOCUS       DAZPBM010000220         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPBM010000220 DAZPBM010000000
VERSION     DAZPBM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.222"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUZQ_003907"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUZQ_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1027483.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHUZQ_003908"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHUZQ_003908"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1027484.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUZQ_003909"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUZQ_003909"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1027485.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUZQ_003910"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUZQ_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1027486.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZPBM010000221         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPBM010000221 DAZPBM010000000
VERSION     DAZPBM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.223"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHUZQ_003911"
     CDS             complement(<1..513)
                     /locus_tag="ACHUZQ_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1027487.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHUZQ_003912"
     CDS             complement(759..1211)
                     /locus_tag="ACHUZQ_003912"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1027488.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUZQ_003913"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHUZQ_003913"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1027489.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUZQ_003914"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHUZQ_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1027490.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHUZQ_003915"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHUZQ_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUZQ_003916"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHUZQ_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1027491.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHUZQ_003917"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHUZQ_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1027492.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZPBM010000222         5613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPBM010000222 DAZPBM010000000
VERSION     DAZPBM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.224"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHUZQ_003918"
     CDS             complement(104..418)
                     /locus_tag="ACHUZQ_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR1027493.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHUZQ_003919"
     CDS             complement(423..638)
                     /locus_tag="ACHUZQ_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1027494.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHUZQ_003920"
     CDS             complement(789..2642)
                     /locus_tag="ACHUZQ_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR1027495.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHUZQ_003921"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHUZQ_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHUZQ_003922"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHUZQ_003922"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHUZQ_003923"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHUZQ_003923"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHUZQ_003924"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHUZQ_003924"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHUZQ_003925"
     CDS             complement(3908..4966)
                     /locus_tag="ACHUZQ_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1027496.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHUZQ_003926"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHUZQ_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..>5613)
                     /locus_tag="ACHUZQ_003927"
     CDS             complement(5539..>5613)
                     /locus_tag="ACHUZQ_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1027497.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1338 a         1583 c         1321 g         1371 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPBM010000223         5559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPBM010000223 DAZPBM010000000
VERSION     DAZPBM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.225"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(78..197)
                     /locus_tag="ACHUZQ_003928"
                     /pseudo
     CDS             complement(78..197)
                     /locus_tag="ACHUZQ_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
     gene            complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHUZQ_003929"
     CDS             complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHUZQ_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1027498.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2416..3051)
                     /gene="nth"
                     /locus_tag="ACHUZQ_003930"
     CDS             complement(2416..3051)
                     /gene="nth"
                     /locus_tag="ACHUZQ_003930"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1027499.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="ACHUZQ_003931"
     CDS             complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="ACHUZQ_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1027500.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="ACHUZQ_003932"
     CDS             complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="ACHUZQ_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1027501.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="ACHUZQ_003933"
     CDS             complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="ACHUZQ_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1027502.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5433..>5559)
                     /locus_tag="ACHUZQ_003934"
     CDS             complement(5433..>5559)
                     /locus_tag="ACHUZQ_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="electron transporter RnfC"
                     /protein_id="HFR1027503.1"
                     /translation="EQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1464 a         1411 c         1365 g         1319 t
ORIGIN      
        1 ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct
       61 taactgatcg gcattacacc gttttcgaga cgttttttca ttaaatccac actgacgagg
      121 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt
      181 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
      241 gctgaacaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
      301 tttacgctac agcagctttc gccgctttgt ctgcagcgtc atcctgcgtc atacggtgca
      361 gtttaggcgc ggtcagcagc atcagtactg caataatggc agtagcgaca ccaatctgca
      421 agaatacgcg accataaact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag
      481 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
      541 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
      601 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
      661 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
      721 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
      781 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
      841 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
      901 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
      961 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
     1021 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
     1081 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
     1141 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
     1201 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
     1261 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
     1321 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
     1381 catagcatgt agaaagcaga gaagatgggt tagctttaaa caggccgtta ccgaccgcaa
     1441 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
     1501 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
     1561 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
     1621 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
     1681 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
     1741 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
     1801 acacttttac ctcttttttt acatcccata ttaacggggg ggctttctcg cgttatgtct
     1861 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
     1921 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
     1981 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaatg gcagattttt
     2041 tcactgcact gagagggggg gtgatggctt aacggcgata tgttctgcta tttggcgttt
     2101 ttactagtga gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata
     2161 tttgttgcgt gtgtaaattt aaattatttt aacctgattc ttgcaatgtt tttacattgc
     2221 gacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
     2281 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
     2341 attcacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
     2401 tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat
     2461 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
     2521 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
     2581 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
     2641 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
     2701 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgctgctc
     2761 cagtaagata cggcaggttt tgatgatatt ttctgctttg ctgttataaa gcccaatcgt
     2821 tttgatatag gttttaaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
     2881 caccgggtaa agtttcgccg tcgccttatt aacactgaca tcggtcgcct gagcggaaag
     2941 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
     3001 attgttctca cgcaggcgag tgaggatctc caggcgtttt gctttgttca tcagacattc
     3061 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagtacggcg ctttttcatt
     3121 ctttcatcaa tcaggtattt tcctgccagc atcagtccca ggccaataaa tgcacctggt
     3181 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt
     3241 gcccagctac ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc gataatttcg
     3301 cgtagtgaac ccagcacgaa catggcgcag gttgcgccca taccaattga aaagccgtcc
     3361 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
     3421 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag
     3481 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
     3541 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
     3601 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
     3661 aacagaggac aaaggccgag caactggacc agcgcagagt tgtttttcca caacccctga
     3721 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
     3781 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
     3841 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
     3901 tgcgcatcat ccgcaccact gattttttta cccgcaaaat gggtgatcca gtcagaaagg
     3961 cgcagttcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
     4021 agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
     4081 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
     4141 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgccagcgta
     4201 ttgttatagc gttcggctgg cagcacctga tcaaataatg ccttttgttg cagactggcc
     4261 tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc
     4321 gctgcaaata gcgctaacgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
     4381 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
     4441 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
     4501 actaataagc ccgcaagcgc gccgaaaata agacgaccac gattggtcgt agaagcggta
     4561 accgggtcag tcaaaataaa gaatgcgccg agcatggtcg caccagacag cagatgaatt
     4621 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
     4681 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacagg
     4741 ccgccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
     4801 tagatcggat attgcataat ctgttcaacc gaatgaccgg cacggacaga ggttttaaat
     4861 gtatccagcg gtgtcgcctg actaatgcca tcaataccta agcgtagtgt gttcatatca
     4921 ccaccactgg cggtatgccc gctgaaaata acctgtatgg cgtcgataaa accagggatg
     4981 ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt
     5041 aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac
     5101 tgtttagcga tgatcaccgc aaacaccgta cccagcacga ccatccacca tggcgcgagg
     5161 gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt
     5221 gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag agtactaacc
     5281 gatgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt
     5341 cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg
     5401 gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac cttctgctgg
     5461 gcggcttttt tggcctgagc gcgggcaata gccgcggcaa ctgccgcttt gcgcggatca
     5521 acctgttctt ctggttctgc gttagtctgt tgctgttcc
//
LOCUS       DAZPBM010000224         5550 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPBM010000224 DAZPBM010000000
VERSION     DAZPBM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.226"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHUZQ_003935"
     CDS             <1..161
                     /locus_tag="ACHUZQ_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1027504.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHUZQ_003936"
     CDS             complement(199..1260)
                     /locus_tag="ACHUZQ_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1027505.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHUZQ_003937"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHUZQ_003937"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1027506.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHUZQ_003938"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHUZQ_003938"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1027507.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHUZQ_003939"
     CDS             complement(3454..4155)
                     /locus_tag="ACHUZQ_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1027508.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHUZQ_003940"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHUZQ_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1027509.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
BASE COUNT         1511 a         1510 c         1403 g         1126 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct
//
LOCUS       DAZPBM010000225         5541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPBM010000225 DAZPBM010000000
VERSION     DAZPBM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.227"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHUZQ_003941"
     CDS             complement(<1..1369)
                     /locus_tag="ACHUZQ_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR1027510.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHUZQ_003942"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHUZQ_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1027511.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHUZQ_003943"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHUZQ_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1027512.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHUZQ_003944"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHUZQ_003944"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1027513.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHUZQ_003945"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHUZQ_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1027514.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZPBM010000226         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPBM010000226 DAZPBM010000000
VERSION     DAZPBM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.228"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHUZQ_003946"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHUZQ_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1027515.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHUZQ_003947"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHUZQ_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR1027516.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHUZQ_003948"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHUZQ_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1027517.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUZQ_003949"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUZQ_003949"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1027518.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUZQ_003950"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUZQ_003950"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1027519.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHUZQ_003951"
     CDS             4447..4812
                     /locus_tag="ACHUZQ_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1027520.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHUZQ_003952"
     CDS             4962..5480
                     /locus_tag="ACHUZQ_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1027521.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1391 c         1241 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZPBM010000227         5456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPBM010000227 DAZPBM010000000
VERSION     DAZPBM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.229"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..879
                     /locus_tag="ACHUZQ_003953"
     CDS             <1..879
                     /locus_tag="ACHUZQ_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFR1027522.1"
                     /translation="FGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPG
                     TKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAET
                     IGNNQMIAVTNNQIETVGVNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRALTVG
                     VAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQT
                     AIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSP
                     PKTPDEKQDTPDMREY"
     gene            947..4942
                     /locus_tag="ACHUZQ_003954"
                     /pseudo
     CDS             947..4942
                     /locus_tag="ACHUZQ_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1333 a         1351 c         1732 g         1040 t
ORIGIN      
        1 tttggcaacc tggcgatacc gcgtgtgggt caggaggtga ttgtggactt cctcaacggc
       61 gacccggacc agccgattat tatggggcgt acctaccacc aggaaaaccg cacacccggc
      121 agcctgccgg ggacgaagac gcagatgacc attcgttcga aaacctataa gggcagcggg
      181 tttaatgaac tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgcgcag
      241 aagaacatga acaccgaggt gctgaataac cgcaccacgg atgtgataaa caaccatgct
      301 gaaaccattg gcaacaatca gatgattgcg gttaccaaca atcagataga gacggtgggc
      361 gttaaccaga tagagacggt gggcgttaac cagatagaga cggtgggcag taaccagata
      421 attaaagtgg gttctgttca ggtggaaacg attggacttg ttcgtgcgct gacggtgggc
      481 gtggcgtatc agacgacggt aggtggcatt atgaacacct cggtggcact gatgcagtcc
      541 tcgcagatcg gtttgcataa atcgttgagg gtcgggctgg gttatgacgt caaagtcgga
      601 aataacgtta ccttcaccgt tggtaaaacg aaaaaggatg ataccgggca gaccgcgatt
      661 tactccgccg gtgagcatct ggagctctgc tgtggtaagg caaggctggt gctgacgaag
      721 gacggacaaa tttttctcaa cggcacaaaa attcatttgc agggtaagga gcaggttaat
      781 ggtgactcac tgttgattaa ctggaactgt gcagcctcga aatccccacc gaagacccct
      841 gatgaaaagc aggatacgcc ggatatgaga gagtactgat cccgtacctg ttcggtattc
      901 agtaacaaac cagcattaaa aataaacaga agaaggaatt tgaatcatga gcggaaaacc
      961 agcggcgcgt cagggagata tgactcagta tggcggtccc attgtccagg gttcggcagg
     1021 tgtaagaatt ggcgcgccca ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc
     1081 gggcaacccg gtaaatccgc tgctgggggc gaaggtgctg cccggcgaga cggaccttgc
     1141 gctgcccggc ccgctgccgt tcattctctc ccgcacctac agcagctacc ggacgaagac
     1201 gcctgcaccg gtgggcgttt tcggccccgg ctggaaagcg ccttctgata tccgcttaca
     1261 gctacgtgat gacggactga tactcaacga caacggcggg cggagcattc actttgagcc
     1321 gctgctgccg ggggaggcgg tgtacagccg cagtgagtca atgtggctgg tgcgcggtgg
     1381 taaggcagca cagccggacg gccatacgct ggcgcggctg tggggggcgc tgcgccggat
     1441 atccggttaa gcccgcatct ttacctggcg accaacagcg cacaggggcc gtggtggata
     1501 ctgggggggg ctgagcgggt gccgggtgct gaggacgtac tgccagcgcc gctgccgccg
     1561 taccgggtgc ttaccgggat ggcggacgct gacgtaccgg cgtgaggccg ccggtgacct
     1621 ggccggggaa atcaccgacg tgacggacgg tgccgggcgg gagttccgtc tggtgctgac
     1681 cacgcaggcg cagcgtgcgg aagaggcccg cacctcttcg ctatcttctt ctgacagttc
     1741 ccgccctctc tcagcctcag cgttccccga cacactgcct ggtaccgaat acggccccga
     1801 caggggtatc cgcctttcgg cggtgtggtt gatgcacgac ccggcatacc cggagagcct
     1861 gcccgctgcg ccactggtgc ggtacacgta tacggaagcc ggtgaactgc tggcggtata
     1921 tgaccgcagc aatacgcagg tgcgcgcttt cacgtatgac gcgcagcacc cgggccggat
     1981 ggtggcgcac cgttacgcgg gaaggccgga gatgcgctac cgctacgacg atacggggcg
     2041 ggtggtggag caactgaacc cggcagggtt aagctaccgc tatctttatg agcaggaccg
     2101 catcaccgtc accgacagcc tgaaccggcg tgaggtgctg catacagaag gcggggccgg
     2161 gctgaaacgg gtggtgaaaa aagaactggc ggacggcagc gtcacgcgca gcgggtatga
     2221 cgcggcagga aggctcacgg cgcagacgga cgcggcggga cggcggacag agtacggtct
     2281 gaatgtggtg tccggcgata tcacggacat caccacaccg gacgggcggg agacgaaatt
     2341 ttactataac gacgggaacc agctgacggc ggtggtgtac ccggacgggc tggagagccg
     2401 ccgggaatat gatgaaccgg gcaggctggt atcggagaca tcgcgcagcg gggagacagt
     2461 acgctaccgc tacgatgacg cgcacagtga gttaccggcg acgacaacgg atgcgacggg
     2521 cagcacccgg cagatgacgt ggagccgcta cgggcagttg ctggcgttca ctgactgctc
     2581 gggctaccag acccgctatg aatacgaccg cttcggccag atgacggcgg ttcaccgcga
     2641 ggaaggtatc agcctttacc gccactatga caaccgtggc cggttaacct cggtgaaaga
     2701 cgcacagggc cgtgaaacgc agtatgagta caacgccgca ggcgacctga ctgccgttat
     2761 caccccggac ggcaaccgga gcgagacaca gtacgatgcg tggggaaagg cggtcagcac
     2821 cacgcagggc gggctgacgc gcagtatgga gtacgatgct gccggacgtg tcatcagtct
     2881 gactaacgag aacggcagtc acagcgactt cagctacgat gcgctggacc ggctggtaca
     2941 gcagggcggc tttgacgggc ggacgcaacg ttatcattat gacctgaccg gaaaactcac
     3001 gcagagtgag gatgaggggc ttgtcaccct ctggcactac gatgcgtcgg accgcatcac
     3061 gcaccggacg gtgaacggcg acccggcaga gcagtggcag tatgacggcc acggctggct
     3121 gagagaaatc agccacctga gcgaaggcca ccgtgtcgct gtccactacg gctacgacga
     3181 taagggccgc ctgacgggcg aacggcagac ggtggagaac ccggagacgg gggaactgct
     3241 gtggcagcat gagacgaaac acgcctacaa cgaacagggg ctggcaaacc gcgtcacgcc
     3301 ggacagcctg ccgccggtgg agtggctgac ctacggcagc ggttacctgg cgggcatgaa
     3361 gctgggcggg acgccgctgg tcgagtatac gcgggacagg ctgcaccgtg agacggtgcg
     3421 caccctcgca ccggacctgg acatccgcat cccgtatgcc accgacccgg cgggaaaccg
     3481 cctgccggac ccggagctac acccggacag cacgctcacg gcgtggccgg ataaccgcat
     3541 cgcagaggat gcgcactatg tttaccacta tgatgaatac ggcagactga cggagaagac
     3601 ggaccgcatc ccgacgggtg tgatacggac ggacgacgag cggacccacc actacgacag
     3661 ccagcaccgc ctggtgttcc acacgcggat acagcatggc gagccactgg tcgagagccg
     3721 ctacctctac gacccgctgg gacggcgaat ggcgaaacgg gtatggcggc gggagcgtga
     3781 cctgacgggg tggatgtcgc tgtcgcgtaa accggaggag acatggtacg gctgggacgg
     3841 cgacaggctg acgacggtac agaccgacac cacacgtatc cagacggtat acgagccggg
     3901 aagcttcacg ccgctcatcc gggtcgagac agagaacggc gagcgggaga aagcgcagcg
     3961 ccgcagcctg gcggagaagc tgcagcagga agggagcgag gacgggcacg gcgtggtatt
     4021 tccggctgaa ctggtgcggc tgctggacag actggaggaa gaaatccggg cagaccgcgt
     4081 gagcagtgaa agccgggtgt ggcttgcgca gtgcgggctg acggtggagc aactggccag
     4141 acaggtggag ccggaataca caccagtgcg aaacgttcat ctttaccact gcgaccatcg
     4201 gggccagccg ctggcgctca tcagcgaaga cggcgatacg gtgtggagcg gggcgtatga
     4261 tgagtggggc aaccagctaa atgaggagaa cccgcatcat gtgtatcagc cataccgtct
     4321 gccggggcag cagtatgatg aggagtcggg gctgtactac aatcgtcacc ggtactatga
     4381 tccgttgcag agacggtaca tcacccagga ccccattggg ctgaaaggtg ggtggaatct
     4441 gtatcagtat ccgctgaatc cggtgagcag gatagatccg ttggggttat tctcatgtga
     4501 gagttgtagt cattttatag atgatataaa taataaactg gataaccttc cgtttaaccc
     4561 gacaaaaatg tttacagcta gtttaaatac tgctaattca gcaaggctgt atgcaagtgg
     4621 catgacaaaa ttcggtataa gtgttgctgc cgatggaacg atcattgctg ccccagtcgg
     4681 tgcagcttct atggctttag gggcgtggaa tataaaatct gcgacagctg catataacag
     4741 agcaaattta caattatcag aatcacttca ggaaaatggt agagataggt gttggagaaa
     4801 tttattgggt attttacctt atggtgagca gtttgatgac cctggtgaac catcaatatt
     4861 agacgtttat ggtgaaaaga ttgagaatat caaagattct ccaatgaagt tttttgaaga
     4921 gcttgggacg ctggggttgt aatgtgtatt tttaatgata tttttatgtt tgtgtttatt
     4981 gttagtagta tacttgtaac cgtgcatttt gtgtattctt tattttttaa aagtggaaag
     5041 gtaaaagata aggtaaacaa atggattaaa gtaatgtgtg atttattttg ggggatagga
     5101 tagtggtgtg atatggataa atataaatta cagtggctgg catatttcga cctgacgccg
     5161 gagtagataa aaaaacagac aggattggag tatagcaagc gtctgacaat ctctcgggac
     5221 agtagtatga tgaagaggcg gggttgtact ataaccgtaa ccggtactac gatccgctaa
     5281 agggcaagta tatgactcag ggaaagatca tcagaagttc actttttgta ctaaataatt
     5341 cgcattttat gtttaaaaat tgagatattc ctcattacct gaagctgttt ttttattgct
     5401 tatacataat caaatactcc ttacataatt aaggagaaca aaatggaaat taaaaa
//
LOCUS       DAZPBM010000228         5455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPBM010000228 DAZPBM010000000
VERSION     DAZPBM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.230"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1334)
                     /locus_tag="ACHUZQ_003955"
     CDS             complement(<1..1334)
                     /locus_tag="ACHUZQ_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1027523.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="ACHUZQ_003956"
     CDS             complement(1380..2138)
                     /locus_tag="ACHUZQ_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027524.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..2883)
                     /locus_tag="ACHUZQ_003957"
     CDS             complement(2170..2883)
                     /locus_tag="ACHUZQ_003957"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1027525.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3057..3749
                     /locus_tag="ACHUZQ_003958"
     CDS             3057..3749
                     /locus_tag="ACHUZQ_003958"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1027526.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHUZQ_003959"
     CDS             complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHUZQ_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1027527.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            5234..>5455
                     /locus_tag="ACHUZQ_003960"
     CDS             5234..>5455
                     /locus_tag="ACHUZQ_003960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027528.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIK"
BASE COUNT         1459 a         1422 c         1315 g         1259 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcga agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtgccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggcgt
     2281 aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt
     2341 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
     2401 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
     2461 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
     2521 gagcagaaag ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca
     2581 ggaaagcaga tagataacca gagcagtaat attgccaggc ggtgttgagg gtttttcgtg
     2641 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
     2701 tgaaatccat tcgccaatta gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
     2761 tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
     2821 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
     2881 cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
     2941 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
     3001 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
     3061 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
     3121 tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
     3181 gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct
     3241 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct agaccagttc
     3301 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
     3361 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
     3421 gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc
     3481 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca
     3541 tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat
     3601 cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg
     3661 atatcgatgc caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg
     3721 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
     3781 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga
     3841 aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
     3901 tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt
     3961 gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
     4021 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
     4081 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg
     4141 atccacacag gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
     4201 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg
     4261 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc
     4321 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
     4381 cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc
     4441 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
     4501 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
     4561 ggggcaatcc ccaccagtac cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg
     4621 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
     4681 ggtttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
     4741 tttttcgtcc cgctccagta gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg
     4801 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
     4861 gcatccatca ccgatgatcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
     4921 ccaatcaagg tgtgcgaact ggaggccggt aaaccgaaga accacgttcc caggttccag
     4981 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgggttga ccccatattc
     5041 agcaacaaat cggttggcaa catatggaca atggcatagg caacgctaag tccgcccaat
     5101 aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
     5161 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
     5221 tagaacaaca caaatgccag agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca
     5281 acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat
     5341 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt
     5401 atttcttgtg tatcagacaa taagcattca ttcaaaacct ttagaaataa tcaaa
//
LOCUS       DAZPBM010000229         5338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPBM010000229 DAZPBM010000000
VERSION     DAZPBM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.231"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            127..1485
                     /gene="mltD"
                     /locus_tag="ACHUZQ_003961"
     CDS             127..1485
                     /gene="mltD"
                     /locus_tag="ACHUZQ_003961"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1027529.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1534..2157)
                     /locus_tag="ACHUZQ_003962"
     CDS             complement(1534..2157)
                     /locus_tag="ACHUZQ_003962"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1027530.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(2161..2961)
                     /locus_tag="ACHUZQ_003963"
     CDS             complement(2161..2961)
                     /locus_tag="ACHUZQ_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1027531.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3202..4116
                     /gene="yafC"
                     /locus_tag="ACHUZQ_003964"
     CDS             3202..4116
                     /gene="yafC"
                     /locus_tag="ACHUZQ_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1027532.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4113..4916)
                     /gene="dkgB"
                     /locus_tag="ACHUZQ_003965"
     CDS             complement(4113..4916)
                     /gene="dkgB"
                     /locus_tag="ACHUZQ_003965"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1027533.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5079..5155)
                     /locus_tag="ACHUZQ_003966"
     tRNA            complement(5079..5155)
                     /locus_tag="ACHUZQ_003966"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5119..5121),aa:Asp,seq:gtc)
BASE COUNT         1401 a         1294 c         1320 g         1323 t
ORIGIN      
        1 gcaacaacct gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
       61 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
      121 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt
      181 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
      241 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
      301 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
      361 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
      421 gagccgtata tgtactggat agcagggcaa gttaaaaaac gtaacatgcc tatggaactg
      481 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
      541 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
      601 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa tatgatgcag
      661 cgtctgaaca aaatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
      721 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
      781 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
      841 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
      901 gctctggcgc gtgtgcacct gagtagcccg gttgaaatgg cgaaggttgc agatatggcg
      961 gggatttccg tcagcaagct gaagacattc aacgctggcg tgaaaggctc cacgctgggc
     1021 gcaagtgggc cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct
     1081 ctcgcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     1141 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagcatcgc ttcacgtctc
     1201 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     1261 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc ggttggcaaa caacagcgat
     1321 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     1381 gtgaacatca aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgat
     1441 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     1501 aaaaggcacc gattcccccg gtgccttttt tatttatgcc gctttatgtg cttctaccat
     1561 gatgatatca ctggtgaaag agccatcagt ctgcaaggaa aaatacgttc tcacctctgt
     1621 cgatgcgctc tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct
     1681 cacgacccat gaagaaaatt ccagcggtaa cttatctgta attaaattat caactatcag
     1741 attggcttca ttgattaacg tcaaccacgc accgctggcg tagtttcgta cgtgagaggt
     1801 atcgcgtaat gcttctaccg cctgtaacca gatgtcgcgc actgggtgac ccggagacat
     1861 tacatccatc acaatcagcc taccgccagg tttcaatatc ctattcactt ctcgcagtgc
     1921 tgcaccaaca tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt
     1981 atcggcaaat ggcagacttg cggcatatcc ctggcgggtg gtgatatttt tcagttgccg
     2041 ggcttcggca gcttgtgcca cgacatccag catgtgggca gataagtcat acgccaccac
     2101 cgcgctcacg ttttgcgcag cgccaaacgt tggtctgccc tgcggcagac ctgacatacc
     2161 ttatttatca ggcttgccgg gactgaattc aacgagtagc ggattgtgat cggaagcgcg
     2221 cgtaaccagt acagaagctt cactgacgtt cagaccacgg tagaaaacaa aatcgagcgg
     2281 gcgaccaaac gcccgacggc gctgatcatc ggtaaaacgc acctggcgca gcgacatttc
     2341 ccgcgcaaag cgatataacg cgttcatcct tctacggctc caggcattga aatctcccgc
     2401 cataatgacc gggccgctgt ggtgagctat ctgatcacca ataggaagta actgcttact
     2461 atagacatcc acgcccagac tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg
     2521 ggtgtcaggt aatggataga ccgtcaccag tgccgacttc gccagacgca aaatgggttc
     2581 tcgttcacgt aacgggcagc aatacactgg atgtgccgcc gaaagggtca ttacgccaga
     2641 aggatgttgt ggcagcacga aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc
     2701 aaactgtacc aactctggcg ttgtttgcgc ttcctgcaat aacaccagat gtgcgtcttt
     2761 gccgtaattc tttaataccg acaaccattc agcgcgttgc tgtttgtata tgttccacac
     2821 caggatccga atacgctctt cggtacttaa cggttctccg ggtggtaatg cctggccgat
     2881 gctcgcaaaa gaccccggcg gtaagatcct ctccgcaggt tgtccggcaa catagcgcat
     2941 ggcataggtg ttttttcgca cttttgactt tgaaacctct attacgtcaa cccttccgca
     3001 ctcgggaggg ttctactgtt atagggattt tagctcactc tttcaacgag caattgtata
     3061 ttgttatgta agcaagtgct tacaccggtc agaaatttgc tcattattga gaacattaaa
     3121 tcggatgaca tatcacgcat cctgatggtt taatgacaaa taaattcaaa atatctcatc
     3181 tattttgcaa caggagcaaa aatgaaagcc acgtcggaag aactcgccat ttttgtttcg
     3241 gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat tagggcaagc aaactcagcg
     3301 gtaagccggg cggtgaaaaa gctggaaatg aaacttggcg ttagcctgct taatcggacc
     3361 acgcggcaac ttagcctgac ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt
     3421 ttgcaggaga tggcagcggc agaatcagaa attatggaga cgcgtaatac accgcgtgga
     3481 ctgttacgga tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttaattaag
     3541 cctttccgtg aacgctatcc ggaagtcact ttatcgctag tctcctccga aacgattatt
     3601 aatttgatcg aaagaaaagt ggatgtcgcg atacgcgctg gtacgttaac ggactccagc
     3661 ttacgtgcca ggccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt
     3721 tcccgctacg ggaagccaga aacgatcgac gatttaaagc aacatgtttg cctgggattc
     3781 actgaacccg cttccctcaa tacctggccg atagcctgta gtgatggaca attacatgag
     3841 gtgaagtacg gtttgtcatc caatagtggg gaaacactga aacagctttg cctgagtggc
     3901 aacgggattg cgtgtttgtc cgactacatg atcgacaaag aaatcgctcg cggagaattg
     3961 gtggagttaa tggcagataa agtgttgcca gtggaaatgc cattcagtgc cgtctattac
     4021 agcgaccgtg cggtaagtac gcgcatccgg gcttttatcg atttccttag cgagcatgta
     4081 aaaacagctc ccggaggagc tgtcagagag gcttaatccc attcaggagc cagaccttcc
     4141 gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca
     4201 agctgtaaat tttgtgcctt aagattactt tccaggtttt tacgtttagt agaagaagga
     4261 attactgagt aaccttcccc catagcccac gccagaatca cttgtgccgg agtcgcattg
     4321 tgtttagctg caatacgagc aataacctca tctttcagtg ccttaccata agccagcgtc
     4381 atataggaag tgatatgaat accgtgctgt ttagcccagg caaccacttt acggttttgc
     4441 agataaggag agagttcaat ctggttggta gcgatgtttt cagcgccaac agcagcaatc
     4501 gctttttcca tcaacgggat cgtgaagttg gaaataccga tctcacgtgt cagaccttgt
     4561 tttttggcct ccagcagcgc ctgcataaac tcttcaacag agacttcatc gtttggtgac
     4621 ggccagtgga ttagcgtcag atcaacataa tcggtacgca atttttgcag gctctctttt
     4681 agactcggga tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag
     4741 agttcatgac gtggcacgcc actttctgca atcgcctgac ctactgcggc ttcgttatca
     4801 tagatttgtg cagtatcaat tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa
     4861 ataacaacgt cgtctttgag acggaaagta cctaaaccaa atgcagggat agccataata
     4921 tgcctctttt aatgctattt tcgttaactg aggtgattat gggcttcaga ctgttgcgga
     4981 aaaagagcat aaaatgcaga ggatttttgc gattctggca ataatagata tacagacgaa
     5041 aaaaaacctc gagcgttaac tcaaggcttc ttaataagtg gcggaacgga cgggactcga
     5101 acccgcgacc ccctgcgtga caggcaggta ttctaaccga ctgaactacc gctccaccga
     5161 attcttttac aaccaccggt tttatgaccg gcttactgct taatttgatg cctggcagtt
     5221 tatgaatcac ttcgtgattc acccttcggg ccgttgcttc gcaacgttca aaagctttcg
     5281 cttttgtcct actctcttat cgagtagtga actgcctcaa aggtcagtgc cattcttc
//
LOCUS       DAZPBM010000230         5281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPBM010000230 DAZPBM010000000
VERSION     DAZPBM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.232"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHUZQ_003967"
     CDS             <1..69
                     /locus_tag="ACHUZQ_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027534.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
     gene            complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUZQ_003968"
     CDS             complement(118..723)
                     /gene="leuD"
                     /locus_tag="ACHUZQ_003968"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1027535.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUZQ_003969"
     CDS             complement(734..2134)
                     /gene="leuC"
                     /locus_tag="ACHUZQ_003969"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1027536.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUZQ_003970"
     CDS             complement(2137..3228)
                     /gene="leuB"
                     /locus_tag="ACHUZQ_003970"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1027537.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUZQ_003971"
     CDS             complement(3228..4799)
                     /gene="leuA"
                     /locus_tag="ACHUZQ_003971"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1027538.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUZQ_003972"
     CDS             complement(4892..4978)
                     /gene="leuL"
                     /locus_tag="ACHUZQ_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1027539.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
BASE COUNT         1141 a         1398 c         1400 g         1342 t
ORIGIN      
        1 gtctactggg taattgcggt tatttctgtt gtcgcattat ttttaaccgc aaaggttaaa
       61 gacgtttgat gacgtggacg atagcggaaa gcccggtcat ttgaccgggc aaggggatta
      121 attcataaac gcaggttgtt ttgcttcata agcggcaatg gcgtcgtcgt gctgcaaggt
      181 aagcccaata ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
      241 gcgataggtt ttctctcccg ctttcacctc ttgcgcttcc agatccacgt cgaaatggat
      301 ccccggattg gctttcacca gcgcaaacag ttcgtccact tctgcatcgc ttaatttcac
      361 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
      421 caccacttta aagccgtagt cggtcaatgc ccagggcgcg tgctcacgcg aggagccaca
      481 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
      541 gaagtccggg tttggctgtt ggcctttttc atcgagaaaa cgccagtcgt taaacagatg
      601 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
      661 atcgacattg gcggcatcca gcggaaccac caggcctgtg tgtttgataa atttctctgc
      721 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagcagcag
      781 cggcagccat tgccgggctg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
      841 agttacggtt gctggtggat gcacaacgtt cgcccggatt cagacggtcg ttgttcatcg
      901 ccagacacat tgagcagcca ggcaagcgcc attcaaaacc ggcttcaata aagattttat
      961 ccagaccttc cgcttccgcc tgggctttta ccgggccaga gccgggaacc accagtgcct
     1021 gcacgcctgg cgcgactttt cgccctttgg cgatctccgc tgccgcgcgt aaatcttcaa
     1081 tgcgcgagtt ggtacaggaa ccgataaaca ctttgtcgat agccacttcg gtcagcggaa
     1141 tgcccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt ttaaccggat
     1201 cggcaaacga agccggatcg ggaatattgt cgttcacgga aatcacctgg cctggattgg
     1261 tgccccaggt gacctgcggt gaaatttctt ctgcttgcag agtgacaacg gtatcgaaag
     1321 ttgcgccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     1381 ctttcggcgc atgcaaacgg cctttgacat agttaaaggt ggtttcgtcc ggtgcaacca
     1441 gaccggcttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     1501 ttaaatcacg gattgcttcg ccgcaaaact ccaccacatg cccggtgccg cctgcgctac
     1561 cggttttacc gataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     1621 cctggacttc aattttcatg gtttttgcgc ggccctgttt cagggtttgc gttgccagta
     1681 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     1741 cggtatgcga gtcgccgcag acaatggtca tccccggcaa ggtgacgccc tgttccggcc
     1801 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagttcg acgccaaatt
     1861 ctttgcagtt tttaatcagc tcctgcatct ggatgcgcgc catttcacca caggcattaa
     1921 tgtctttcgt ctgggtagag acgttgtgat ccatagtggc gaaggttttg cccggctgac
     1981 gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc acttcatgca
     2041 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     2101 cgtcgaataa tttttcgtat aacgttttag ccatgattac accccttctg ccacatagcg
     2161 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatcccc
     2221 ggtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgc aagccgcatc
     2281 atcggcatcc aggctgtagc gcagcagcag tgccagcgac agaatttgcg caatcgggtt
     2341 ggcgatgttt ttgcctgcga tatctggtgc tgagccgcct gccggttcat acagaccaaa
     2401 accttgctcg ttcaggctgg cggaaggcaa catccccatc gagccagtga tcattgcgca
     2461 ctcgtcagac agaatgtcgc caaacaggtt ggagcacagc agaacgtcaa actgtgatgg
     2521 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgccagtt cgacatccgg
     2581 gtattccgtg gcgatctcgt tgacgatttc acgccataaa atagaggatt gcagcacgtt
     2641 agctttgtcg attgaggtga ctttgtggcg acgtttacga gcagattcaa acgcgatgcg
     2701 ggcaatacgc tcgatctcaa aacggtgata cacctcggta tcaaacgctt tttcatattg
     2761 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     2821 gcacaggatg tcgaagccgt tagcggcaat gtctgcacgc agcggacaga atgcttccag
     2881 cccctgatac agttttgccg ggcgcaggtt gctgaataat ttgaagcgct tacgcagagg
     2941 cagcagcgcg ccgcgttctg gttgctggtc tggtggtaaa tgttcccact tcgggccgcc
     3001 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg caggcggcag
     3061 tggttgcccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatgcg
     3121 catcgcaaag cggttgcgca cggcattcag cactttcagc gcctgggtca tcacttccgg
     3181 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
     3241 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
     3301 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
     3361 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
     3421 gcatctttac cgtgaccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
     3481 gtgatgcggt taattgcctg atagacggca tcgaccggac cgttaccgtt ggcggcttct
     3541 gctttgactt cttcgccaca ggccagtttg acggcggcgg tggcgatatc gttagagcca
     3601 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
     3661 aaggccagcg cctccagatc gtaatcaaac acctgacctt ttttgtccgc cagcttcagg
     3721 aaagcatcgt acaaattgtc taaattatat tcactttctt tataccccat ctcatccatg
     3781 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggttcaga
     3841 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagcac gccatcctgg
     3901 tggataccgg aggagtgagc gaatgcgctg ctgccaacaa tggctttgtt tgccgggatc
     3961 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattaatg
     4021 gcggtgtgga cgttgagaat atccttacga actttaatcg ccatgatgac ttcttccagc
     4081 gaacagttac cggcacgctc gccgatcccg ttcattgcac cttccacctg gcgtgcaccg
     4141 gcatgtaccg ccgccagtga gttgccgacc gccaggccta aatcgtcgtg ggtatgtacg
     4201 gaaataatgg ctttgtcgat gttaggtacg cgttcataca ggccgctgat gattccggcg
     4261 aactcaagcg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
     4321 ttaatcgccg cttcgaccac tcgcgccaga tcggcaatgg gtgtacgccc ggcatcttcg
     4381 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
     4441 tcgatcacct cgtccagggt gctgcgcagc ttggtggcga tgtgcattgg ggaggtagca
     4501 ataaaggtat gaatacggaa ggcttcggcg actttcaggg attcggccgc cacgtcgata
     4561 tctttttcca cgcagcgagc taacgcacat acgcggctgt ttttaacctg gcgggcgatg
     4621 gtttgcaccg attcaaaatc gcccggcgaa gagacgggga aaccgacttc catcacgtca
     4681 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
     4741 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
     4801 gtttaggtcc ttgtctcttt tagagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
     4861 gcgcgggttt tttgtttgac tgcgtgctgg cttaatgctg gatgccgctc actcgtctac
     4921 cgcgcaaaga agatgcgttt agtagtagta gaccgataaa gcgaacgatg tgagtcatta
     4981 aatcagctcc agatgaatgc gatatgcttt tagagttact ggatacaaaa acggatgtca
     5041 accctgacgc aataaaaacg tcccgccagc gtgagttctg catccgtaaa attagctaat
     5101 tgtgctgcgg tggttaaagt aagcgatatt aatttctgct taactaccga cgcttttcat
     5161 cggttgacat atttcagcat aaatttttgc atctaatcaa cgaggaaaaa ggggacaaaa
     5221 tgcacgcgtt gcaaaaccta tcctgatgat ttgtattgaa ttatatgttt tgcgattttt
     5281 t
//
LOCUS       DAZPBM010000231         5274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPBM010000231 DAZPBM010000000
VERSION     DAZPBM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.233"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(25..426)
                     /gene="crl"
                     /locus_tag="ACHUZQ_003973"
     CDS             complement(25..426)
                     /gene="crl"
                     /locus_tag="ACHUZQ_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1027540.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(484..1728)
                     /gene="frsA"
                     /locus_tag="ACHUZQ_003974"
     CDS             complement(484..1728)
                     /gene="frsA"
                     /locus_tag="ACHUZQ_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1027541.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(1820..2278)
                     /gene="gpt"
                     /locus_tag="ACHUZQ_003975"
     CDS             complement(1820..2278)
                     /gene="gpt"
                     /locus_tag="ACHUZQ_003975"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1027542.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2539..3996
                     /gene="pepD"
                     /locus_tag="ACHUZQ_003976"
     CDS             2539..3996
                     /gene="pepD"
                     /locus_tag="ACHUZQ_003976"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1027543.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(4053..4667)
                     /gene="prfH"
                     /locus_tag="ACHUZQ_003977"
     CDS             complement(4053..4667)
                     /gene="prfH"
                     /locus_tag="ACHUZQ_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1027544.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(4664..5274)
                     /locus_tag="ACHUZQ_003978"
                     /pseudo
     CDS             complement(4664..5274)
                     /locus_tag="ACHUZQ_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1294 a         1418 c         1295 g         1267 t
ORIGIN      
        1 tgtgatacat ccggcacatt tcactcacgc cgttaacttc accggttcat cacgaaaatc
       61 atccgccggt tccagcttca gattcagcgt cgtcagcagt tcacgcagct tctcgtgaaa
      121 ctcacgtagg gtgtgctcca gtcgttcaac cacttcagtg tcttttaccg gaacactctt
      181 ccagtcgcct gctttatcga acagaccaaa ctggtaactg taggtaaaac gggattcctg
      241 cgcttcaagc tccatccacc agccccagaa ctcacgcact tccggtgccg gtttcacgtt
      301 gacgcataca gccagacaat cgaaaaagaa tcgattatct tcgcacttac cttcacgaat
      361 atacgggcct agtgcggtaa attttttgat caatctgctc ttcgggtgtc cactcggtaa
      421 cgtcattgca atctcctgtt gtgatgcaac tgttttacca aattggcaaa ttttagcaaa
      481 tttttaacac aagcgttttt cgatccagcc ggtgatttcc tgaagacctt tgtcaaaatt
      541 ccgatacacc gggttaaatg ggatctctaa taatttaccg tcagcagatg atgaggtgat
      601 taagcgtgag tcctcttccg ggctgaacgg atcgttcttc cagtagcctg ataacattgg
      661 cgttgggcag cgacgcccca gcaatccttg cacttttaat gaatagcgat tcagctccac
      721 gcgtaacgct tcgtcggaag catcatgcat ccccaaacga ctcgccagaa cgtcaagata
      781 catttccggc acctgttgct ggcacttaaa atcactcaac agggtatgaa ctaccggacc
      841 aagacaggca accgctttca gacgcggcga ttcaagatat gccagacgca cggcaacgtt
      901 agcgccgaaa cggaaaccaa aggccgcgac gcgagtgtga tccacccacg gtacgttagg
      961 cagagccttt aaaacgtgct gatgcaacag gctggagtcc tgggtgagct tccattttga
     1021 tgaaaagccc accgacggca tatcaatagt cagcatcgca atgccgcgcg gcgcaaaata
     1081 acgttcatac aggctgtaat agtccgtctg catcgcatcc agaccaccac acattaatac
     1141 tgtcgggaac gggccatcgc ctttcggcat atgcaaaaag ccggtgatgg gcgcaccgcc
     1201 gggtacggta aactccatct gccgcatcgt gcccggcaga cgctgggcgg cttcttcata
     1261 ggcacggttt gacaaagcct gcgcttgctc ggccaggtca tctcctttca gatgaggata
     1321 ggcagcaatg ttgtacaacg tagccgcatg cagccagtga cgaccactga gttgcggatc
     1381 ttcttccgca caggcttttt gttgccacac catcgcctgg atggcccact cataaatcca
     1441 gttgccacca cggtagccaa tcaccgtatc gtataaatca tcgtcggtac gttcggcatc
     1501 gctcatcaca atacgcgcct ggacgtcgag gatttcacgt gggtcaatgc cgcgccagat
     1561 ccacatcaga cggttaatca tgcgatacca gtgagggatg gttttaccat caagggccga
     1621 ctgcacaggc ggttgtgcgc cgtgattaaa gcggcggact agcgtcgagg tttcaggatg
     1681 tttaaagcgg ggtttgaaca gggtttcgct caggtttgcc tgtgtcatgg atgcagcctc
     1741 cagaatactt actggaaact attgtaaccc gcctgaagtt aaaaagaaca acgcccggca
     1801 gtgccaggcg ttgaaaagat tagcgaccgg agattggcgg gacgaatacg acgcccatat
     1861 cccacggctg ttcaatccag gtatcttgcg ggatatcaac aacatagtca tcaaccagcg
     1921 gacgaccagc cggttttgcg aagatggtga caaagtgcgc ttttggatac atttcacgaa
     1981 tcgcaaccgc agtaccaccg gtatccacca ggtcatcaat aacgatgaag ccttcgccat
     2041 cgccttctgc gcgttttagc actttaagct cgcgctggtt gtcgtgatcg tagctggaaa
     2101 tacaaacggt atcgacatga cgaataccca gttcacgcgc cagtaacgca cccggtacca
     2161 gaccgccacg gcttacggca ataatgcctt tccattgttc agaaggcatc agtcggcttg
     2221 cgagtttacg tgcatggatc tgcaacatgt cccaggtgac gatgtatttt tcgctcatgt
     2281 gaagtgtccc agcctgttta tctacggctt aaaaagtgtt cgaggggaaa ataggttgcg
     2341 cgagattata gagatctggc gcactaaaaa ccagtatttc acatgagtcc gcgtcttttt
     2401 acgcactgcc tctccctgac gcgggataaa gtggtattct caaacatatc tcgcaagcct
     2461 gtcttgtgtt gacaacattt tctgctaacc ctgtgacctg caatactgtt ttgcgggtga
     2521 tcgacaagga gacttaacgt gtctgaactg tctcaattat ctccacagcc gctgtgggat
     2581 atttttgcca aaatctgttc tattcctcac ccgtcctatc atgaagagca actcgctgaa
     2641 tacattgttg gttgggcaaa agagaaaggt ttccatgtcg aacgcgatca ggtaggtaat
     2701 atcctgattc gtaaacctgc taccgcaggt atggaaaatc gtaaatcggt cgtcttacag
     2761 gcccacctcg atatggtgcc gcagaaaaat aacgacaccg tgcatgactt cacgaaagat
     2821 cctatccagc cttatattga tggcgaatgg gttaaagcgc gcggcaccac gctgggtgca
     2881 gataacggca ttggtatggc gtctgcgctg gcggttctgg ctgacgaaaa cgtggttcac
     2941 ggcccgctgg aagtgctgct gaccatgacc gaagaagccg gtatggacgg tgcgttcggc
     3001 ttacagagca actggttgca ggctgatatt ctgattaaca ccgactccga agaagaaggt
     3061 gaaatctaca tgggttgtgc ggggggtatc gacttcacct ccaacctgca tttagatcgt
     3121 gaagcggttc cagctggttt tgaaaccttc aagttaacct taaaaggtct gaaagggggt
     3181 cactccggcg gggaaatcca cgttggcctg ggtaatgcca acaaactgct ggtgcgcttc
     3241 ctagcgggtc atgcagaaga actggatctg cgccttatcg atttcaacgg cggcacactg
     3301 cgtaacgcca tcccgcgtga agcctttgcg accattgctg tcgcagctga taaagtcgac
     3361 gtcctgaaat ctctggtgaa tacctatcag gagatcctga aaaacgagct ggcagagaaa
     3421 gagaaaaatc tggccttgtt gctggactct gtagcgaacg ataaagctgc cctgattgcg
     3481 aaatctcgcg atacctttat tcgtctgctg aacgccaccc cgaacggtgt gatccgcaac
     3541 tcagacgtgg caaaaggtgt ggtcgaaacc tccctgaacg tcggtgtggt gaccatgact
     3601 gataataacg tagaaattca ctgtctgatc cgttctctga tcgacagcgg taaagactac
     3661 gtggtgagca tgctggattc gctgggtaaa ctggctggcg cgaaaaccga agcgaaaggc
     3721 gcatatcctg gctggcagcc ggacgctaat tctccggtga tgcatctggt acgtgaaacc
     3781 tatcagcgtc tgttcaacaa aacgccgaac atccagatta tccacgcggg cctggaatgt
     3841 ggtctgttca aaaaaccgta tccggaaatg gacatggttt ctatcgggcc aactatcacc
     3901 ggtccacact ctccggatga gcaagttcac atcgaaagcg taggtcatta ctggacactg
     3961 ctgactgaac tgctgaaaga aattccggcg aagtaattat ttgatttgct gccggatggc
     4021 gtttaatcgc cttccggcag tttcatcctt cattatcctt cgataaaagc catccctgta
     4081 aatgtccgtc tcgggttgcc acgttcaatc tggtgatgga acattcgccg ctgcgatttc
     4141 agcaccgcgc tattttcctg ttgctgctgc tccagcttcc aggcaatcag caatcgtgcc
     4201 agccgcttgt tagcatgctg actacgctct gactgaacct tcacgctaat accggatgcc
     4261 aaatgcgtgg cgcgtaccgc cgagtcggtt ttattgacat gttgaccgcc cggccccgac
     4321 gaacgcaacg tctcataacg gattgcatcc gattgttcct gctcatcagc ggtaaaacgc
     4381 ccaatgccca gaaaccagtt tttgcgccca tgatgaggcc gatacggact cggacaaatc
     4441 cactgaatag tgccgcacca gctttcgctt aatgcccatg cgttatcgcc atccagagaa
     4501 accagagctg aacgcagcgt gtcagagtag cggcccgttt ctgtttccaa caccgttacc
     4561 gcgacgtctt gtcgggcagc ttctttaatt aatcgctcca gcgccttttt cactgcgaga
     4621 caacattctt ccggcccctg cgctgaggag agttgtagca agatcatcct tttttccctc
     4681 cactgttttt gagcgttagc accggacgca gccgcgcgac aggaataatt aaaccagcct
     4741 gcaccagaca ttgcaccacg ctttcagccg atttataggc ttgtggcgct tcttcaaaga
     4801 tgagttgttt atcgcgacaa attacccgac tgccaagttc agtacgtgag agctgcgtcg
     4861 ctgtgtattt cgctgccaga cgccctttac actcggtgcg cccccattta cgcccagccc
     4921 catgcgccag cgagtggagc gttttttcgt tcgcaacagg ctgtacaagc taggagtaat
     4981 caccgagtga accgggaata atcaccagac cgttgtcatc cggcgtggca cctttacgat
     5041 gcaaccagcc ctgttgatcg ccgatttgac acgcgctaac aaagttatgc gccacatcca
     5101 gaaccggact tccggtggcc ttaacctgtt gcattatgcg taaagagatc agctggcgat
     5161 taatacgcgc aaacgccagc gcatcgtcat gttccgcaat ataccgtagc gcgtcgtcac
     5221 tgccttcagg caaaccatga tgcgaaaacg aggcaatatg ccgctgtaaa atag
//
LOCUS       DAZPBM010000232         5202 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPBM010000232 DAZPBM010000000
VERSION     DAZPBM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.234"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            435..1409
                     /gene="hemB"
                     /locus_tag="ACHUZQ_003979"
     CDS             435..1409
                     /gene="hemB"
                     /locus_tag="ACHUZQ_003979"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1027545.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1515..2366)
                     /gene="tauD"
                     /locus_tag="ACHUZQ_003980"
     CDS             complement(1515..2366)
                     /gene="tauD"
                     /locus_tag="ACHUZQ_003980"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1027546.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2363..3190)
                     /gene="tauC"
                     /locus_tag="ACHUZQ_003981"
     CDS             complement(2363..3190)
                     /gene="tauC"
                     /locus_tag="ACHUZQ_003981"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1027547.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3187..3954)
                     /gene="tauB"
                     /locus_tag="ACHUZQ_003982"
     CDS             complement(3187..3954)
                     /gene="tauB"
                     /locus_tag="ACHUZQ_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1027548.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3967..4929)
                     /gene="tauA"
                     /locus_tag="ACHUZQ_003983"
     CDS             complement(3967..4929)
                     /gene="tauA"
                     /locus_tag="ACHUZQ_003983"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1027549.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1267 a         1457 c         1277 g         1201 t
ORIGIN      
        1 gttacagacg aatggttcaa ttattctttc ccctaagtcc gaatgtggac atatataatt
       61 tatggagaaa aacacgcatc aaattgataa aaggacgata tttcaacatg ttgcagataa
      121 tgttattttt gatacatacg cgttataaat atcaaaaaca caaaccagat aaatatctta
      181 tcaatataca acaaagtttg aattattcaa tttatagcag cacaaccaaa cgccaaaaaa
      241 aataataacc aagagctata aataaagatt aaaaatattt tcagtgaata tgaaattatt
      301 gctatttcta ttacaaagat gtcctggtct ctctttcatt ctcttcttgt caacatcgcg
      361 acaactttcg taaaacactc ctgccccgct tcaggtatac tgtgcacctt aattccacaa
      421 acatcaggca gaccatgaca gacttaatcc aacgccctcg tcgcctacgc aaatctcctg
      481 cgctgcgcgc tatgtttgaa gagacaacac ttagccttaa cgacctggtg ttgccgatct
      541 ttgttgaaga agaaattgac gactacaaag ccgttgaagc catgccaggc gtgatgcgca
      601 ttccagagaa acatctggca cgcgaaattg aacgcatcgc caacgccggt atccgttccg
      661 taatgacttt tggcatctct caccataccg atgaaaccgg cagcgacgcc tggcgggaag
      721 atggactggt ggcgcgtatg tcgcgcatct gcaagcagac tgtgccagaa atgatcgtca
      781 tgtcagacac ttgtttctgt gaatacactt ctcacggtca ctgcggtgtg ctgtgcgagc
      841 atggcgtcga caacgacgcg actctggaaa atttaggcaa gcaagccgtg gttgcagctg
      901 ctgcaggcgc agacttcatc gccccttccg ccgcgatgga cggccaggta caggcgattc
      961 gtcaggcgct ggacgctgca ggctttaaag atacggcgat tatgtcgtat tcgaccaagt
     1021 tcgcctcttc cttttatggc ccgttccgtg aagctgccgg aagcgcatta aaaggcgacc
     1081 gcaaaagcta tcagatgaac ccaatgaacc gtcgcgaggc gattcgtgaa tcactgctgg
     1141 atgaagccca gggcgcagac tgcctgatgg ttaaacctgc tggagcgtac ctcgacatcg
     1201 tgcgtgagct gcgtgaacgt actgaattgc cgattggcgc gtatcaggtg agcggtgagt
     1261 acgcgatgat taagttcgcc gcgctggcgg gtgctataga tgaagagaaa gtcgtgctcg
     1321 aaagcttagg ttcaattaag cgtgcgggtg cggatctgat tttcagctac tttgcgctgg
     1381 atttggctga gaagaagatt ctgcgttaat ttttttcctc tcagaaaggg ggagttaacc
     1441 tgttgaaagt acggtgaacg ccccctcacc ctaaccctct ccccaagggg gcgagggaac
     1501 cgtccgcact cgtatcaccc cgcccgataa aacggtttat ccccaaggat cgtcgcccga
     1561 tgcataatcc gccgctgtgg caggtaatcg gcattggcat agtgctgagt cacgcggtta
     1621 tcccaaatcg caatatcatt cggttgccag cgccaacgca cctgaaactc cggtttagtg
     1681 atatgggcga ataaaaagcc taacaaggct tcgctctctt tctcgctgac atcgacaatt
     1741 cgcgtagtaa agccttcatt cacaaacagc gcctgtttac cgctcaccgg atgcgttcgc
     1801 accaccggat gtagcaacgg aggatttttc gcgaccgcct cgcgccagcg ttgatgttcc
     1861 tcctcggttt tgcggtattt gtattccggg aacgatttac ggaaatcatg ctccgcacgc
     1921 agcccgctca gcagctggcg gaagggaaca gagagcgcct cataggccgc aataccgctg
     1981 gtccagagcg tatcaccgcc ggtcgaaggt aactctttag ctgccagaat cgcccctgcg
     2041 ggtggcgttt caataaatgt cacatcggta tgccagttgt cgttatccgg cggattatcg
     2101 ttatgggtat ccagcacgat gatctcgtca accccttcgg catgagggta aacagggtga
     2161 atatgcagtt cgccaaaacg ctgggccagc gcgcgttgct gctgcggcgt aatagcttga
     2221 tcgcgcagaa ataccacctg atagcgcagc accgcatggt aaagctgttc aaactgatta
     2281 tcgcttaacg ggcgcgtcag gtcggcaccc gaaatttgtg cgccgatata cggccccagc
     2341 ggggtaatac tcagacgttc actcattgta cttctccatg ccagggcgtc agacggcgct
     2401 gtaacgcgcg cagacccagt tctaaaagaa aggcgataat cgcaatcacc gcgatccccg
     2461 ccagtaccac gtcagtcgcg agaaattcac ccgctgactg aaccataaat cctaaaccgc
     2521 gtgtcgcggc aatcagctcc gccgctacca gcgtagacca gcccacaccc agaccaatgc
     2581 gtaatccggt gaggatttcc ggcagcgcgc cgggcaaaat gacaaaccac agcacctgcg
     2641 cacggctggc acccagcgac tgggcggcac gaatgcgaac ctgctgcgcg cttttcaccc
     2701 ccgccagcac cgacatcgcc accggtgcaa aaatcgctaa atagatcaat aagatcttcg
     2761 aggtttcacc aataccaaac cagatcacca tcagcggcaa ataagccagc ggcggcaccg
     2821 gacgataaag ttcgattacc ggatccagaa tgccgcgcac cgtcgggctt agccccatgg
     2881 cgatcccgac cggaataccg atcaccaccg ccgcgagtag cgccagcaca atgcgcgtca
     2941 ggctggctgc cagatgctgc cacagcgtgg cgtccataaa tccctgcggt ccggcaatgg
     3001 tgagcagttt tgccagtact tgttgcggcg gcggcaaaaa cagcgggcta atcagttgca
     3061 gcgccgccac cgtccaccat acagtgagta aaaccgctaa cgtgccaatg cttaaggtca
     3121 cctgacgcga gagcggccag cgccatttca gccgccgcga atgcagtttt tcattaatga
     3181 gcacactcat gagaacgcct cccgttgctc aaatacgcgg cttaaaacat attcgcgcat
     3241 ggcgatgaat tgtggatcgg acttgatgct gcggctcgac tctcccgcaa caaagcggcg
     3301 agcaaagttg agcggcagcc gctccagcac acggccaggg ccggatgaaa gcagaaccag
     3361 ttcagtcgcc ataaacaccg cttcttctat atcgtgggta atcaacagca cctgcttgcc
     3421 cgtctcctgc cagagtttca gcagcagagt ttgcatctgg tcgcgggtga aggcgtccag
     3481 cgcaccaaac ggttcgtcga gtaataacag ctggggattc gccgccagcg cgcgggcaat
     3541 ccccacccgc tgacgttgac caccggaaag ctgccagata tagcgttttt ctgcgccttc
     3601 cagccccact tttttcagca tctggtgcgc gatttccagt cgctgcattt tctctatacc
     3661 tgccaattgc aggccgaacg ccacgttgtc ctgtacattg cgccacggta gtagcccttc
     3721 attctgaaaa accaccccgc gctctgctcc cggtccctca atacgcttac ccgccagttg
     3781 aatgctgcca tgctgataag gcacaaaacc ggcaatcaga ttcagcaggg tggttttacc
     3841 gcagccggac ggccccagca ccaccagtag ctcgccgctt tccagcgtca ggttgatatc
     3901 ttccagtgcc ggtttgccgc cataatcggc atagagatga gagatttgca gcatctgcgc
     3961 ctccttttat tgcacgaagc gcgaggtaac gtactggctg taatcattcg ctacagccgg
     4021 gaccttgccc tgctctttca aaaactgcgc ggtgtcgatg attgctttgt tcaccggtcc
     4081 ggtcagttct gccgtttgtt gctgcggcgt cagataggta ttccccttca ccagccccgg
     4141 aacgtcacct tccggcacgc cgcttaaacg cgccagtttg ctgatgtttt ccggctgttt
     4201 cagccacgcg tctgggttag caatgtacgg ttgctgagca tcgatggcgc ttttagcgaa
     4261 cgctttcacg acctcaggat gtttctcggc aaaatctttg cgcaccaccc agacgtccag
     4321 cgttggcgca ccccactgcc cgacctgttc agaatcggtc aacaccttgc cgtctttttc
     4381 cagggcgtta accgccggtg cccagacata agcaccatca atatcacccc gctgccatgc
     4441 ggcgataatc gcgggcggct gcaggttcac aatctccact tgccctggtt taatccccca
     4501 gtgtttcagc gccgccagca ggctgtagtg ggtggtggag ataaacggta cggcaatgcg
     4561 tttgccaatc aaatcttcgg gtttgctgat agttttcttt accaccagcg cttcggagtt
     4621 acccagtttt gacgccagca agaagacttc aatcggcacc tgttggctgg ctgcaaccgc
     4681 taatgggctg gaaccgaggt tgccgatttg cacgtcgcca gaagccagcg cccgcacgat
     4741 gctggctccg ctgtcaaact tacgccagtc cacgctcgcc ccgctctctt tagcaaaggt
     4801 gttgtcggcc tgagccactt tcgccggttc ggctgaggtt tgatacgcca cggtgacgtt
     4861 caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt tacgcgatga
     4921 aattgccatg attgtctgct cccctgtctt gttatgggag cagtattcag gaataaaaac
     4981 attcattaaa agaattagtc gttatcgcac agatgatttt attcttagca aaaaaacggc
     5041 aaaggaaaga ttaaatatgc aattactccg ttctaacaat tgggaaaatt actacacttc
     5101 attttagtga tgcataaaca accacattac gagatttatt acttcattgt ttctatcaat
     5161 acatataata aaactattat attcgcattg tatatatcac tg
//
LOCUS       DAZPBM010000233         5182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPBM010000233 DAZPBM010000000
VERSION     DAZPBM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.235"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHUZQ_003984"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHUZQ_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1027550.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUZQ_003985"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUZQ_003985"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1027551.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHUZQ_003986"
     CDS             2475..4124
                     /locus_tag="ACHUZQ_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1027552.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5182)
                     /locus_tag="ACHUZQ_003987"
                     /pseudo
     CDS             complement(4278..5182)
                     /locus_tag="ACHUZQ_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1122 a         1206 c         1398 g         1456 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac caaggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg ac
//
LOCUS       DAZPBM010000234         5114 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPBM010000234 DAZPBM010000000
VERSION     DAZPBM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.236"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHUZQ_003988"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHUZQ_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="ACHUZQ_003989"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHUZQ_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHUZQ_003990"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHUZQ_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUZQ_003991"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUZQ_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1027553.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHUZQ_003992"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHUZQ_003992"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHUZQ_003993"
     CDS             complement(2927..3859)
                     /locus_tag="ACHUZQ_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1027554.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUZQ_003994"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUZQ_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1027555.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1262 a         1181 c         1184 g         1487 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     5101 caaaattaac acct
//
LOCUS       DAZPBM010000235         5097 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPBM010000235 DAZPBM010000000
VERSION     DAZPBM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.237"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHUZQ_003995"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHUZQ_003995"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1027556.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHUZQ_003996"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHUZQ_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1027557.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHUZQ_003997"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHUZQ_003997"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1027558.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..>5097
                     /gene="uidB"
                     /locus_tag="ACHUZQ_003998"
     CDS             3749..>5097
                     /gene="uidB"
                     /locus_tag="ACHUZQ_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR1027559.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQ"
BASE COUNT         1246 a         1204 c         1344 g         1303 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagca
//
LOCUS       DAZPBM010000236         5084 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPBM010000236 DAZPBM010000000
VERSION     DAZPBM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.238"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..488
                     /locus_tag="ACHUZQ_003999"
     CDS             <1..488
                     /locus_tag="ACHUZQ_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1027560.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="ACHUZQ_004000"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="ACHUZQ_004000"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1027561.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHUZQ_004001"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHUZQ_004001"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1027562.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHUZQ_004002"
     CDS             complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHUZQ_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1027563.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHUZQ_004003"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHUZQ_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1027564.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHUZQ_004004"
     CDS             complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHUZQ_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1027565.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4693..>5084
                     /gene="dgcM"
                     /locus_tag="ACHUZQ_004005"
     CDS             4693..>5084
                     /gene="dgcM"
                     /locus_tag="ACHUZQ_004005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR1027566.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGF"
BASE COUNT         1382 a         1297 c         1210 g         1195 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt ttta
//
LOCUS       DAZPBM010000237         5083 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPBM010000237 DAZPBM010000000
VERSION     DAZPBM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.239"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            143..694
                     /locus_tag="ACHUZQ_004006"
     CDS             143..694
                     /locus_tag="ACHUZQ_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1027567.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            754..3258
                     /locus_tag="ACHUZQ_004007"
     CDS             754..3258
                     /locus_tag="ACHUZQ_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1027568.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWDDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3274..4023
                     /locus_tag="ACHUZQ_004008"
     CDS             3274..4023
                     /locus_tag="ACHUZQ_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1027569.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            4025..>5083
                     /locus_tag="ACHUZQ_004009"
     CDS             4025..>5083
                     /locus_tag="ACHUZQ_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027570.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT         1589 a         1029 c         1028 g         1437 t
ORIGIN      
        1 tttaagtttt acttaatcca tatttagata aactaatttc ataaaacccg aaaagtaaaa
       61 tataatattt gaatgattag caatccttaa ataacagtta ctaatcattc attttctact
      121 tatatttata taaaggaata ttatgtctgc ttttaaaaag tcacttcttg ttgctggcgt
      181 agcaatgatt ctttccaata acgtatttgc tgacgaaggt cacggcattg ttaaatttaa
      241 aggtgaagtt atctctgctc catgttcaat taaaccaggt gacgaagact taactgtaaa
      301 tctgggtgaa gtagcagata ccgttctgaa aagcgaccag aaatctttgg ctgagccttt
      361 cactattcac ctgcaagact gcatgttgag ccagggtgga accacttatt caaaagcgaa
      421 ggttactttc accacagcga atactatgac gggtcagact gacctgctga aaaacaccaa
      481 agaaactgaa atcggtggtg ccacaggcgt tggcgtacgt attctggata gccagagcgg
      541 cgaagtgact ttaggtactc cggtagtaat tacattcaac aatactaaca gctatcagga
      601 actgaacttc aaagctcgta tggaatcccc gggcaaagat gctaccccgg gcaacgttta
      661 cgctcaggct gactataaga ttgcttacga gtaatcacat taggccgggt tcgcccggcc
      721 acatcattaa cggattaatg ataagtggaa cagatgtata aaaaattaaa attaaccaca
      781 ataagcgaat tgattaaaaa tatttattgt tcattatccg ttctcatcat tggttgtgcg
      841 tcagcttatg ccgttgaatt caacaaagat ttaatcgaag ccgaagatcg tgaaaacgtt
      901 aacctttccc aatttgaaac tgatggccaa ttacccgtag gtaaatactc attaaacgct
      961 cttattaata ataaaaggac gccaatccat ctggacctgc aatgggtatt aattgataac
     1021 caaactgcag tttgtctgac tccagagcaa ttaacattat taggctttac tgatgagatt
     1081 attgaagagg ctcagcaaaa cctgatcgat ggttgttacc ctatcgaaaa agaaaaacaa
     1141 attacaactt atctcgataa agggaaaatg caattatcca tatctgcacc tcaggcatgg
     1201 ttaaaataca aagatgcaaa ctggacgcct cctgaacttt gggatcatgg tattgctggg
     1261 gcatttcttg actacaattt atatgcctct cattatgcac cacatcaggg cgataattca
     1321 caaaatataa gttcctatgg gcaggctggg gttaatcttg gggcctggcg cctgcgtact
     1381 gattaccagt acgatcagtc atttaacaat ggcaaaagcc aggcgaacaa cctggatttt
     1441 ccgcgtattt atttgtttcg cccaatccca gcaattaatg caaaactaac tataggtcaa
     1501 tacgatactg aatcctctat tttcgactct ttccattttt ctggcgtttc gttgaaaagc
     1561 gatgagaata tgttgccgcc tgatctacgt ggttacgcac cgcaaatcac gggtgttgca
     1621 caaacgaatg caaaggtcac tgtctcacag aacaaccgta ttatttatca agaaaatgtt
     1681 cctcctggcc catttgctat tactaattta ttcaatacat tacaggggca actggacgtc
     1741 aaggttgaag aagaggacgg gcaagtcacg caatggcaag ttgcatctaa tagtattcct
     1801 tatctgacgc gtaaagggca gattcgctac accactgcta tgggtaaacc gaccagcgtt
     1861 ggtggtgatt ccttacaaca acccttcttc tggactggtg aattctcatg gggttggctg
     1921 aacaatgtat ccctgtatgg tggttcagtt ttaacaaacc gtgattatca atctctggct
     1981 gccggcgttg gctttaacct taactcattg ggttcattat cttttgatgt cacacgatct
     2041 gatgctcagt tgcataatca ggataaagaa acgggttata gctaccgcgc taactattca
     2101 aaacgttttg aatctaccgg tagccagctc actttcgctg gttaccgttt ctctgataaa
     2161 aactttgtga caatgaatga atatatcaat gacactaacc attacatgaa ttatcagaat
     2221 gaaaaagaga gttatattgt cacgtttaac cagtatcttg aatcattaag gttaaataca
     2281 tacgtaagtt tggctcgtaa tacttactgg gacgccagca gtaatgtgaa ttattcatta
     2341 tcacttagcc gcgattttga tatcgggcca ttaaaaaacg tctccgcttc tctaacattt
     2401 agccgaataa actgggaaga agacaaccag gatcaactgt acctaaatat ttcgattccc
     2461 tggggaacca gtagaacatt gagctatggt atgcaacgaa atcaggataa caagatttcg
     2521 catactgctt cgtggtatga ctcttccgat cgaaataatt cctggagcgt ttctgcttca
     2581 ggcgacaatg atgaattcaa agatatgaaa gcgtcactac gcgccagtta tcagcataat
     2641 accgagaacg gtcgtctcta tctctccggg acatcacagc gagacagtta ttattctctg
     2701 aatgccagtt ggaatggttc attcacggcg actcgtcacg gagccgcttt ccatgactat
     2761 agtggtagtg ctgactcgcg ttttatgatc gacgcagatg gtgcagaaga tattccgttg
     2821 aacaataaac gcgcggtaac taatcggtat ggcatcggag ttattccatc agtcagcagt
     2881 tacataacaa catcattaag cgttgatacc cgaaatcttc cagaaaatgt ggatatcgaa
     2941 aactcggtta tcaccaccac cttaaccgaa ggtgctattg gctacgccaa acttgatacc
     3001 cgcaagggct atcaaatcat gggggttatt cgcctggcag atggtagtca cccaccactg
     3061 gggattagcg taaaagataa aaccagccat aaagaattag gactggttgc tgatggcggc
     3121 tttgtatacc tcaacggcat tcaggatgac agcaaactta ctttacgctg ggatgataaa
     3181 tcctgtttta ttcaaccacc caatagcagc aacttaacca ccggaacggt tattttaccg
     3241 tgtattagcc aaaattaatt caggatgaaa ctaatgcgct acttaaacac aaagaatctc
     3301 attgcagcag gagtattact cgcctgcatg agcagcattg cctggggtgc catcatcccg
     3361 gaccgtaccc gtatcataat gaatgagtca gataaagggg aagcattaaa acttactaat
     3421 caaagcaaaa atctacccta tctggcgcaa acatggatag aagatacgaa aggcaataaa
     3481 tcacgtgact ttattgtcac ggttccccct atggtacgtt taaatcccag tgagcaaatt
     3541 caaataagaa tgataagtca ggaaaaaatt gctcaactgc ctaatgacag agaaacgctc
     3601 ttctatttta acgtgcgaga aataccgcca aaaacagata aaaaaaatgt gatgcaagtc
     3661 actatgcagc acgcattgaa actattctgg cgaccaaaag ctattgaatt agaagatgat
     3721 ggggtaatgt cttacgaaaa agttgaaatc atccgtagaa atgatggttc gatccgcttt
     3781 aacaataaga tgccttatca cgttacgctg ggttatatcg gaacaaacgg aatcacaatg
     3841 ttgccacaaa cgcaaagttt aatggtaacc ccttatagcc atgcagatac gcagtttaaa
     3901 aatgttccgt cagcttttca ggttggttat atcaatgatt ttggtggatt aagtttttat
     3961 gaaataaact gcccgacggt taataactct tgtaatgttt ccgtagccaa acgagataaa
     4021 taatatgcgc tacttattaa ttgtaattat atttattata ggttttagtg cattacctgt
     4081 atgggcaatg gactgctatg ctgaacatga agggggaaat accgtagtca ttggttacgt
     4141 accaagaatt tctatcccca gtaatggtaa aaaaggtgat aagatctggc aaagcagtga
     4201 gtattttatg aatgtcttct gtaataatgc attacctgcc ccatcacctg gagaagaata
     4261 cccatcagcc tggactaata taatgatgtt tttaccaggc ggtcaggact tttataatca
     4321 gaactcctat atttttggcg taacatacaa tggagttgat tatgattcga ctgcccctaa
     4381 tgctatcgca gcaccagaat gcattgatat aaaaggagcg ggtaccttta ataatcacta
     4441 taaaaacccc gctgtctgta gtggaggtcc tgagcctcaa ttatcagtaa cctttccagc
     4501 tcgcgtacaa ctctatatta aattagcaaa aaatgccaat agagttaata aaaacctggt
     4561 gttacctgat gaatatatcg ctctagagtt taaaggtatg agtggcgcag gagctataga
     4621 agtagacaaa aatttgacct tcaggattcg tggattaaat aatattcatg tccttgactg
     4681 ctttgttaat gtcgcactgg aaccagctga tggcgttgtt gactttggta aaataaattc
     4741 ccgaacaatt aaaaatacca gcgttagtga gacgtttagt gtagtcatga caaaagatcc
     4801 tggtgcggcc tgtactgagc aattcaatat tttagggagc tttttcacta cggatatttt
     4861 gagtgattat agccatctgg atatgggtaa tggactgcta ttgaagatat ttcataaaga
     4921 tggaacagca acggaattta accgcttctc acaatttgct tctttttcat cgtctagtgc
     4981 accttcggtc accgcaccat tcagggcaga acttagtgcg aacccggcag agactgttgt
     5041 tgagggaccg tttagtaaag acgtaatcct caaaatcacc tat
//
LOCUS       DAZPBM010000238         4856 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPBM010000238 DAZPBM010000000
VERSION     DAZPBM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.240"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUZQ_004010"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHUZQ_004010"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1027571.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUZQ_004011"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHUZQ_004011"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1027572.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1086 a         1356 c         1249 g         1165 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atgttttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaaacga
     4801 gcaggaaaaa gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa cagaag
//
LOCUS       DAZPBM010000239         4844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPBM010000239 DAZPBM010000000
VERSION     DAZPBM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.241"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(34..954)
                     /locus_tag="ACHUZQ_004012"
     CDS             complement(34..954)
                     /locus_tag="ACHUZQ_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1027573.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHUZQ_004013"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHUZQ_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1027574.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="ACHUZQ_004014"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="ACHUZQ_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1027575.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHUZQ_004015"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHUZQ_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1027576.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1307 a         1081 c         1140 g         1316 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAZPBM010000240         4830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPBM010000240 DAZPBM010000000
VERSION     DAZPBM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.242"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(71..2140)
                     /gene="glyS"
                     /locus_tag="ACHUZQ_004016"
     CDS             complement(71..2140)
                     /gene="glyS"
                     /locus_tag="ACHUZQ_004016"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1027577.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2150..3061)
                     /gene="glyQ"
                     /locus_tag="ACHUZQ_004017"
     CDS             complement(2150..3061)
                     /gene="glyQ"
                     /locus_tag="ACHUZQ_004017"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1027578.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3156..3455)
                     /locus_tag="ACHUZQ_004018"
     CDS             complement(3156..3455)
                     /locus_tag="ACHUZQ_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1027579.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3630..4625
                     /gene="wecH"
                     /locus_tag="ACHUZQ_004019"
     CDS             3630..4625
                     /gene="wecH"
                     /locus_tag="ACHUZQ_004019"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1027580.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4667..>4830)
                     /locus_tag="ACHUZQ_004020"
     CDS             complement(4667..>4830)
                     /locus_tag="ACHUZQ_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1027581.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         1103 a         1267 c         1227 g         1233 t
ORIGIN      
        1 aaggttgctg tattatttag cgataataaa aaaagcctgc cagatggcag gctttttaat
       61 aacggcgtta ttactgcaac agcgaaatat ccgcaacgcg caggaacagt tcgcgcagtt
      121 tctccagcat ggtcagacgg ttgagacgca attctttgtc atcaaccatg accatcactt
      181 tatcgaagaa agcatcaacc ggctcacgca gctctgccag ttcgaccagc gcatcctggt
      241 aacgaccttc cgcaaagtac ggctccagct tgtcacgtag caccacaacc tgcatcgcca
      301 gtttaatttc ttccggctct ttcagggtag aggcattcac gcggtcgctc agcacttcgt
      361 cagatttcgc cagaatgtta gatacacgtt tgttcgccgc cgccagtgca gcagctgcat
      421 ccagggtacg gaaatgcgat accgctttca tacgggcatc gaaatcagcc ggacgagtcg
      481 gacgacgcgc cagtaccgcc tggatggtgt caacggtgta accttcgtcc tgataccagg
      541 cgcggaagcg accgagcata aagtcgataa catcatcaac tacgttggcg ttagtcagct
      601 tatcgccata cagacgcact gcttcttcgg tcagcgtttg cagatcaagg ttgaggttct
      661 tctcaacgat aattcgcagc acgccaagtg cggcacgacg cagcgcaaac gggtctttgt
      721 cgcctttcgg atgctgaccg ataccgaaga tacccgccag agtatccatc ttatcagcaa
      781 tcgccagcgc acaagctacc gggttggacg gcaggtcatc accggcaaag cgcggctgat
      841 actgctcatt cagcgcgacg gctacgtctt ccgcttcgcc atcgtgacgc gcgtagtgca
      901 tccccataac gccttgggtg tcggtgaact cgaagaccat gttggtcatc aggtcacact
      961 tagacagcag gcccgcacgg gttgcgtggt taacgtcagc gccaatctgt tcagcaatcc
     1021 agccagccag cgcctggatg cggtcagttt tgtcacgcag cgtacccagc tgttgctgga
     1081 acagcacggt ttgcaggcgc ggcaggttat cttccagacg ttttttacgg tcggtattga
     1141 agaagaactc ggcatccgcc agacgtggac gaacgacttt ctcgttacca gagataattt
     1201 gctgcggatc tttcgattcg atgttggcaa cgaagatgaa gttcggcagc agtttgccat
     1261 cgttcgcata caccgggaag tatttctggt cacctttcat ggtgtaaacc agggcttcag
     1321 ccggcaccgc gaggaatttc tcttcgaatt ttgcggtcag aacgaccggc cactccacca
     1381 gcgaagccac ttcttccagc aggctttcgc ttaagtcagc gttaccgcca atcttacgcg
     1441 ctgcttcttc ggcatcggct ttaatcttcg ctttacgttc ttcgtaatcg gcgatgactt
     1501 tcccgcgctc acgcagaatt tccggatact gatcggcgtt gtcgatggtg aattccggtt
     1561 cgcccataaa gcggtggccg cgaatcacgc gatcggactg aatgcccaga atggttgccg
     1621 gaatgacttt gtcgcccagc agcagggtca cggtgtgcac cggacgcacg aagtgcacgt
     1681 cgcttgcgcc ccaacgcatc agtttcggga tcggcagttt cgccagcgaa gtcgcaacca
     1741 tattcggcag tagtgcttca gtgctttcgc ccttcacatg ggcgcgatac agcagccatt
     1801 cgcctttatc ggtagtcaga cgctcagcct ggtcaacggt aataccgcaa ccacgcgccc
     1861 aaccttctgc cgctttgctc ggtttgcctt cagcgtcgaa cgcctgggca atcgccgggc
     1921 cgcgtttttc gatttcacga tctggttgcg cttccgccag gttagctact ttcaacgcca
     1981 gacgacgcgg agcagcaaac cattgaacgg tgccgtgtgc gaggccagcg ttatccagct
     2041 ccgcagtaaa gttcgcagca aaggactcag ccaggctgcg cagtgctttt ggtggcagct
     2101 cttcagtgcc gatttccacc agaaaagttt tctcagacat agccgcctct tacttatctt
     2161 tgttgcacat cgggaagccg agggcttcac gggaagcgta gtatgcttct gccactgctt
     2221 tggtcagggt gcgaatgcgc agaatgtagc gctgacgctc agtgacggag atggctttac
     2281 gcgcatccag caggttgaag ctgtgggcgg ctttcagaat acgctcgtag gctggcagcg
     2341 gcagcggatt ttccagcgcc agcagctgct gcgcttcttt ctcgtactgc tcgaagcagg
     2401 tgaacaggaa atccacatcc gcgtattcga agttgtaagt ggactgctcc acttcgttct
     2461 gatggaacac gtcgccgtag gtggttttac ccagcgggcc gtcgctccag accaggtcgt
     2521 aaacgctgtc tacgccctga atgtacatgg ccagacgttc cagaccgtag gtgatctcgc
     2581 cggtaaccgg tttacactcc agaccaccaa cctgctggaa gtaagtgaac tgcgtcactt
     2641 ccatgccgtt tagccacact tcccagccca gtccccaggc acccagagtc gggttttccc
     2701 agttatcttc cacgaaacgg atgtcgtgaa tagtcgggtc catgcccagc tctttcagag
     2761 aaccgaggta cagctcctga atattgtccg gcgatggctt aatgaccacc tggaactgat
     2821 agtagtgctg taaacggttg gggttttcgc cgtagcgacc atcggtcggg cgacgagaag
     2881 gctgaacata agcagccgcc atcggttctg gccccagcgc gcgcagacag gtcattgggt
     2941 gagaggttcc cgcgccgact tccatgtcca atggttgaac aatggtgcag ccctggcgag
     3001 cccagtaatc ctgtaaggtc aggatcaagc cctggaaggt cctggtatca aacttttgca
     3061 tattatttcg tgctggatac gtgtggattt aaaggaaggg atcagtatac ccgctggatg
     3121 gaagatatac agtacgaaac gggaaaaagc agggcttaac gcatggaaag atgcaaaaaa
     3181 tggccatctg cgtcaaaaga acagacaaac tgctctttac ggaaggtata accgcgcatt
     3241 tcatagcttc cctggaactg ttcgaaggcg gtgacgtcga ttttttgctc gccggtgtta
     3301 taacgttttg ccgcctgatc cttgcataac gcttccatat tcaacgaacg cagaggatca
     3361 acctttaccc gttgtgcttt ctgcacgggt gacggtgtag accaacccac tagcaccata
     3421 agcgccattg ccggaaagaa agcgttcatc atcattaaat taccgtctgg tttgcaggtg
     3481 agtcttatta tttatatcgg tatagtggct gatatcttac gaatttcctt aagcctctat
     3541 aagctgtagt gataaaactc agattattcc agtgcgccaa ccaaatgatt tatcggtcag
     3601 acttttttta accagataca gagacacaga tgcagcccaa aatttactgg attgataacc
     3661 tgcgagggat agcgtgttta atggtggtga tgattcacac cactacctgg tatgtgacca
     3721 atgctcatag tgttagcccc gtcacatggg atatcgccaa tgttctgaat tctgcctctc
     3781 gtgtcagcgt gccgctattt ttcatgattt ccggctatct cttttttggc gaacgcagcg
     3841 cccagccgcg ccatttcttg cgtatcggct tatgtctgat tttttatagc gcaatcgcac
     3901 tgctctacat tgcgctgttt acctccatca atatggagtt agcgctgaaa aacctgctgc
     3961 aaaagccagt gttttaccac ttgtggtttt tcttcgcgat tgcggtgatt tatctggttt
     4021 caccgctgat tcaggtgaag aacgtcggcg gaaaaatgtt gctggtgcta atggcggtga
     4081 ttggcattat cgctaaccca aacacagtgc cgcagaaaat tgacggtttt gaatggctgc
     4141 caattaactt atatatcaat ggcgatactt tttactacat tctgtatggc atgttgggcc
     4201 gcgctatagg gatgatggac acacagcata aagcactgtc gtgggtgagc gccgcgctgt
     4261 ttgcgacggg ggtttttatt atctctcgcg ggacattata tgaattgcag tggcgcggaa
     4321 attttgccga tacctggtat ctttactgtg ggccgatggt ttttatctgc gcaatcgcgc
     4381 tattgactct ggttaaaaac acgctggata cgcgtaccat tcgcggactt ggcttaatct
     4441 cccgccattc attgggtata tacggattcc acgccttgat tatccatgcg ctgcgcaccc
     4501 ggggaattga gcttaaaaat tggccaatac tggatattat ttggatcttt tgcgcgacgt
     4561 tggcagcgag tttgttactt tctatgctgg tacaacgaat cgacagaaac agattagtga
     4621 gttaagtaaa agcccgttca cattggactg accgggctta cgtgaattat tcgctgtatt
     4681 cttcctgggt aacctgtgtt tcttttggcg ggtttattcc ggcatcacga atattcttct
     4741 gcaccgctac tgcaccaaac aagcttaaga agaaagccag tccataaaaa cctttttcac
     4801 tgagtaataa tgtcgcattc cacagaccta
//
LOCUS       DAZPBM010000241         4828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPBM010000241 DAZPBM010000000
VERSION     DAZPBM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.243"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(103..546)
                     /locus_tag="ACHUZQ_004021"
     CDS             complement(103..546)
                     /locus_tag="ACHUZQ_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1027582.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            703..1632
                     /gene="metA"
                     /locus_tag="ACHUZQ_004022"
     CDS             703..1632
                     /gene="metA"
                     /locus_tag="ACHUZQ_004022"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1027583.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1901..3502
                     /gene="aceB"
                     /locus_tag="ACHUZQ_004023"
                     /pseudo
     CDS             1901..3502
                     /gene="aceB"
                     /locus_tag="ACHUZQ_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3532..4823
                     /gene="aceA"
                     /locus_tag="ACHUZQ_004024"
                     /pseudo
     CDS             3532..4823
                     /gene="aceA"
                     /locus_tag="ACHUZQ_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1203 a         1271 c         1223 g         1131 t
ORIGIN      
        1 aggccatcct gacggatggc ctttttgcat tggcgcagaa aaaaatgccg ggtgcgacgc
       61 tggcgcgtct tctccagcct gggcaggcac ggcaggggca gcttattcct ccacatacgc
      121 cagattcagc aacggatacg gtttccccaa atcgtccacc tcagagcgtc ccgtaacctt
      181 aaaacccacc ttcttataga acccaaccgc ctgctcattt tgctcattaa cgttggttgt
      241 cagttccggt gccatcgaga gcgcatgctc caccagcatc cgacccacgc cgcagccgca
      301 cacatcagga tcgataaaca gcgcatccat atgctgccca cttaacaaca taaacccaac
      361 cggctgatcc cgttcattaa ccgcgaccca caacggcgct tccggcagga aggaacgaac
      421 tagcacctcc agctcggccc gatactctgc tgataaaaaa tcgtgtgtgg catcgacaga
      481 acgacaccaa atcgcaacga gttcctcccc ttcctcatgc cgtgagcggc gaatactaat
      541 aaccattttc tctccttttc gtcattctta tattctaacg tagccttttc cttgaaactt
      601 tctcaccttc aacatgcagg ctcgacattg gcaaattttc tggttatctt cagctatctg
      661 gatgtctaaa cgtataagcg tatgtagtga ggtaatcagg ttatgccgat tcgtgtgccg
      721 gacgagctac ccgccgtcaa tttcttgcgt gaagaaaacg tctttgtgat gacaacttct
      781 cgtgcgtctg gtcaggaaat tcgtccactt aaggttctga tccttaacct gatgccgaag
      841 aagattgaaa ctgaaaatca gtttctgcgc ctgctttcaa actcaccttt gcaggtcgat
      901 attcagctgt tgcgcatcga ttcccgtgaa tcgcgcaaca cgcccgcaga gcatctgaac
      961 aacttctact gtaactttga agatattcag gaacaaaact ttgacggtct gatagttacc
     1021 ggcgcgccgc tgggcctagt ggagtttaat gatgtcgctt actggccgca gatcaaacag
     1081 gtgctggagt ggtcgaaaga tcacgtcacc tcgacgctgt ttgtctgctg ggcggtacag
     1141 gccgcgctca atatcctcta cggcattcct aagcaaactc gcaccgacaa actctctggc
     1201 gtttacgagc atcatattct ccatcctcat gcgcttctga cgcgtggctt tgatgattca
     1261 ttcctggcac cgcattcgcg ctatgctgac tttccggcag cgttgattcg tgattacacc
     1321 gatctggaaa ttctggcaga gacggaagaa ggggatgcat atctgtttgc cagtaaagat
     1381 aagcgcattg cctttgtgac gggccatccc gaatatgatg cgcaaacgct ggcgcaggaa
     1441 tatttccgcg atgtggaagc cggactagac ccggatgtac cgtataacta tttcccgcac
     1501 aatgatccgc aaaacaaacc gcgcgcgagc tggcgtagtc acggtaattt gctgtttacc
     1561 aactggctca actattacgt ctaccagatc acgccatacg atctacggca catgaatcca
     1621 acgctggatt aatcttctgt gatagtcgat cgttaagcga ttcagcacct tacctcaggc
     1681 accttcgggt gcctttttta tttccgaaac gcacctcagc aagtgaataa attttattca
     1741 tattgttatc aataagttat caagtatttt taattaaaat ggaaattgtt tttgattttg
     1801 cattttaaat gagtagtctt agttgtgctg aacgaaaagc gcacaacgat ccttcgttca
     1861 cagtggggaa gttttcggat ccatgacgag gagctgcacg atgactgaac aggcaacaac
     1921 aaccgatgaa ctggctttca caaggccgta tggcgagcag gagaagcaaa ttcttactgc
     1981 cgaagcggta gaatttctga ctgagctggt gacgcatttt acgccacaac gcaataaact
     2041 tctggcagcg cgcattcagc agcagcaaga tattgataac ggaacgttgc ctgattttat
     2101 ttcggaaaca gcttccattc gcgatgctga ttggaaaatt cgcgggattc ctgcggactt
     2161 agaagaccgc cgcgtagaga taactggccc ggtagagcgc aagatggtga tcaacgcgct
     2221 caacgccaat gtgaaagtct ttatggccga tttcgaagat tcactggccc cggactggaa
     2281 caaagtgatc gacgggcaaa ttaacctgcg tgatgcagtt aacggcacca tcagctatac
     2341 caatgaagca ggcaaaattt atcagctcaa gcccaatcca gcggttttga tttgtcgggt
     2401 acgcggtcta cacttgccgg aaaaacatgt cacctggcgt ggtgaggcaa tccccggtag
     2461 cctgtttgat tttgcgctct atttcttcca caactatcag gcactgttgg caaagggcag
     2521 cggtccctat ttctatctgc cgaaaaccca gtcctggcag gaagcggcct ggtggagcga
     2581 agtcttcagc tatgcagaag atcgctttaa tctgccgcgc ggcgccatca aggcgacgtt
     2641 gctgattgaa acgctgcccg ccgtgttcca gatggacgaa atccttcacg cgctgcgcga
     2701 ccatattgtt ggtctgaact gcggtcgctg ggattacatc ttcagctata tcaaaacgtt
     2761 gaaaaactat cccgatcgtg tcctgccaga cagacaggcc gtgacgatgg ataaaccatt
     2821 cctgaatgct tactcacgcc tgctgattaa aacctgccat aaacgcggtg cttttgcgat
     2881 gggcggcatg gcagcgttta ttccgagcaa agatgaagag cgtaataacc aggtgctcaa
     2941 caaagtaaaa gcggataaag cgctggaagt caataacggt cacgatggca catggatcgc
     3001 tcacccaggc cttgcggaca cggcaatggc ggtattcaac gacattctcg gctcccgtaa
     3061 aaatcagctt gaagtattgc gcgaacaaga cgcgccgatt actgccgatc agctgctggc
     3121 accttgtgac ggtgaacgca ccgaagaagg tatgcgcgcc aacattcgcg tggctgtgca
     3181 gtacatcgaa gcgtggatct ccggcaacgg ctgcgtgccg atttatggcc tgatggaaga
     3241 tgcggcgacg gctgaaattt cccgtacctc gatctggcag tggatccatc atcaaaaaac
     3301 gttgagcaat ggcaaaccgg tgaccaaagc cttgttccgc tagatgctgg gcgaagagat
     3361 gaaagtcatt gccagcgaac tgggcgaaga acgtttctcc cagggccgtt ttgacgatgc
     3421 cgcacgcttg atggaacaga tcaccacttc cgatgagtta attgatttcc tgaccctgcc
     3481 aggctaccgc ctgttagcgt aaaccaccac ataactatgg agcatctgca catgaaaacc
     3541 cgtacacaac aaattgaaga attacagaaa gagtggactc aaccgcgttg ggaaggcatt
     3601 actcgcccat acagtgcgga agatgtggtg aaattacgcg gttcagtcaa tcctgaatgc
     3661 acgctggcgc aactgggcgc agcgaaaatg tggcgtctgc tgcacggtga gtcgaaaaaa
     3721 ggctacatca acagcctcgg tgcactgact ggcggtcagg cgctgcaaca ggcgaaagcg
     3781 ggtattgaag cagtctatct gtcgggatgg caggtagcgg cggacgctaa cctggcggcc
     3841 agcatgtatc cggatcagtc gctctatccg gcaaactcgg tgccagctgt ggtggagcgg
     3901 atcaacaaca ccttccgtcg tgccgatcag atccaatggt ccgcgggcat tgagccgggc
     3961 gatccgcgct atgtcgatta cttcctgccg atcgttgccg atgcggaagc cggttttggc
     4021 ggtgtcctga atgcctttga actgatgaaa gcgatgattg aagccggtgc agcggcagtt
     4081 cacttcgaag atcagctggc gtcagtgaag aaatgcggtc acatgggcgg caaagttttg
     4141 gtgccaactc aagaagctat tcagaaactg gtcgcggcgc gtctggcagc tgacgtgacg
     4201 ggcgttccaa ccctactggt tgctcgtacc gatgccgatg cagcggatct gatcacctcc
     4261 gattgcgacc cgtatgacag cgaatttatt accggcgagc gtaccagtga aggcttcttc
     4321 cgtactcatg cgggcattga gcaagcgatc agccgtggcc tggcgtatgc gccatatgct
     4381 gacctggtct ggtgtgaaac ctccacgccg gatctggaac tggcgcgtcg ctttgcacaa
     4441 gctatccacg cgaaatatcc gggcaaactg ctggcttata actgctcgcc gtcgttcaac
     4501 tggcagaaaa acctcgacga caaaactatt gccagcttcc agcagcagct gtcggatatg
     4561 ggctacaagt tccagttcat caccctggca ggtatccaca gcatgtggtt caacatgttt
     4621 gacctggcaa acgcctatgc ccagggcgag ggtatgaagc actacgttga gaaagtgcag
     4681 cagccggaat ttgccgctat accttcgtat ctcaccagca ggaagtgggt acaggttact
     4741 tcgataaagt gacgactatt attcagggcg gcacgtcttc agtcaccgcg ctgaccggct
     4801 ccactgaaga atcgcagttc taagcaac
//
LOCUS       DAZPBM010000242         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPBM010000242 DAZPBM010000000
VERSION     DAZPBM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.245"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUZQ_004025"
     tRNA            6..81
                     /locus_tag="ACHUZQ_004025"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUZQ_004026"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUZQ_004026"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUZQ_004027"
     CDS             complement(299..1057)
                     /locus_tag="ACHUZQ_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1027584.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUZQ_004028"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUZQ_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1027585.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHUZQ_004029"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHUZQ_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4459)
                     /locus_tag="ACHUZQ_004030"
                     /pseudo
     CDS             complement(3427..4459)
                     /locus_tag="ACHUZQ_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1317 a         1241 c         1147 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcatggcga
     3721 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3781 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3841 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3901 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3961 ccttgaaata gtcggcgacg ttgagttcgg tatctgtacc cgcctgaata cagacggtag
     4021 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4081 cgtcgtaatc agtgacgcca gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc
     4141 aggtcgtatt acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt
     4201 ttgcagtgag cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4261 cacgacaaac atctacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4321 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg
     4381 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4441 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4501 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccatcttt
     4561 tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4621 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4681 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggagg tgtgaaagtt
     4741 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaggtaatga
     4801 ctc
//
LOCUS       DAZPBM010000243         4762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPBM010000243 DAZPBM010000000
VERSION     DAZPBM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.246"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..678
                     /locus_tag="ACHUZQ_004031"
     CDS             <1..678
                     /locus_tag="ACHUZQ_004031"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR1027586.1"
                     /translation="KFLFLMSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGA
                     ATEHDTLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTS
                     YWLRHIYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQM
                     VCCADTLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSY
                     RIAQRLS"
     gene            1042..1928
                     /locus_tag="ACHUZQ_004032"
                     /pseudo
     CDS             1042..1928
                     /locus_tag="ACHUZQ_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1928..2218
                     /locus_tag="ACHUZQ_004033"
     CDS             1928..2218
                     /locus_tag="ACHUZQ_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027587.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2539..2829
                     /locus_tag="ACHUZQ_004034"
     CDS             2539..2829
                     /locus_tag="ACHUZQ_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027588.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2829..3248
                     /locus_tag="ACHUZQ_004035"
     CDS             2829..3248
                     /locus_tag="ACHUZQ_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027589.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            3416..3739
                     /locus_tag="ACHUZQ_004036"
     CDS             3416..3739
                     /locus_tag="ACHUZQ_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027590.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            4169..>4762
                     /locus_tag="ACHUZQ_004037"
     CDS             4169..>4762
                     /locus_tag="ACHUZQ_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027591.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1557 a          878 c          977 g         1350 t
ORIGIN      
        1 aaatttttat ttcttatgtc aattgagcat attttaatcg ggaagcatca tgggggttct
       61 gattactgga gaactccatt tttgttattt aagaacttac accgtgaatt tattttcagt
      121 ctagatggtg ctgcaactga gcatgacacg ctgttaccac gattcaccga tgatataagc
      181 cgtcaatcat gggttagtga aaaggtcttc tgtaacccgc cgtacagcga tattccgtcg
      241 ttcctcttaa aagcgtctga agcggattta gtagctttcc tgataccgca tagagccaac
      301 accagctact ggcttaggca catttacagc aataaccact gtcatgaaat ccgtattctt
      361 catcgtgcag ttaaatacct tccaccagca gggcataaca ggcttactat taggtcaccg
      421 tttccaagtg cagtcgttgt ttttaaaaaa gaaccacgta agcatgaaat tacacagatg
      481 gtttgctgtg ctgatacatt gctaccgtta accatcatta accgtggtgg actaagagga
      541 agacccacca tttacccacc tgagacgctc gacagcttca taaaactata caggcaagga
      601 aaacccatta agtacattgc agatgctctc agaatgcctc tgagcacgtc atacaggatt
      661 gctcaaaggt tgagctaaaa ttctcataaa tatggtcatt tacaagacag gtgattcaga
      721 tgttaagaag tatccctgtg aacaataaat gaccaatcag taatcattct gtgaaataat
      781 tgttgcaatt tataatcaca tctgttatag tttctatatg ggctgataaa aaagttcatg
      841 tcttgaagtg gtaacacttg gggaggtgtc agatggttgg cgtatattaa cctctcattc
      901 agggtatacc tcagcgtttt aatcatcgta ctgtttcagg catggacttc tttatcagtc
      961 catcaatccc ccagatattt taatcacaca ggctaatcgg ttttatccgg ttggcctttt
     1021 cacgtatgag gtaatttatg aatgtctaaa tataacccaa atcagtttta taaatttgat
     1081 gtgcgaatct ctcaactttc agaagaaatt aacgcagcag caagattgat ttatatggtc
     1141 atggtccata gctgtgactt ctatgaagag aaatacaaat catattcacc aagtattgac
     1201 catctcgctt atatggctgg tgtatcaaag agaacagcaa tcagagccat tgagacactt
     1261 gagaaacacg gtttaattca ggtgcaaaag aatcaggggc gtcaaagcac ttatcgtgta
     1321 ttcacactgg atgataagcc ggaattactt gtaagaccta catcacagga agttgcagcc
     1381 tcagtaagac aaaatgacac cactaccagt gacaaaatgt catgggggta gtgacaaaat
     1441 gtcacccgtg ttagagagat aagttattag ataaaacatt agataataat attacagata
     1501 agaataaaga gactggactt aatgatgata ttaaagaaga tgactttctt tctttggatg
     1561 aattaaatga ttttctctct atgaatgaat ctgaccttga agtaagctct aaaaagactt
     1621 ctaaacaaga cagagaaatc actgatgtga gtgttgatag tgaccataaa aacacacagt
     1681 gccccgccac tgatgatgag ttggacccct tcgatgatga agaggatgaa cgtgaaaaag
     1741 ccattaagag ggcagagaag aaaaagagat tcgagcttgc aagacaaaag aacaaagtat
     1801 cgggtttaga cccattcgat gacagtgaag atgattatta tctttcaccg aaaaagaaac
     1861 aaactcagga tgacaagaag caaaaaatta aaccgaagat gtattttgcc ggtggaggaa
     1921 ttaagtaatg aaagaatatc acggtgaaaa gagatacaaa gattacctgt taaagcgtta
     1981 cagcatttct cgtgaaggtc atcttatgaa ggatactcac ggggaagttt accggattag
     2041 accaaagaaa gaagggaggg gttatttctt ttacgatggt gtgacagatt taaagattga
     2101 cgctttgagg tttgcagtgc tgtatcattt tgatatctgg aattcaatcc accagctacg
     2161 attaaaagac ggtgacccca gcaacttaaa ggccaccaat attatcacaa aacgttagtt
     2221 tgttttacca tcatcaatcg taattgtgat ttcatatttg aaaagcttaa tgctgaaatt
     2281 gaatcgtgta cttctggcga taatcatttt taaaagctga tacacaagaa gcaccagaaa
     2341 cacaaagcaa cctaagcttg cgaatctgtg agattcagca attgtttcaa gaaagcccat
     2401 taattcccct tatttatcaa agggaatgac ctgtatcagc cccctacaac catattaaca
     2461 tgtaaatgag ttgttgacaa tatgttataa atatgttaaa gttagttata agtcaaagat
     2521 tttaagaagg aaaatttaat gagacacaca gttacacgcc ttttgccaga ggcatattcc
     2581 agactggcag caatcaaaga caaataccca gccatgagcc acacagcact tgttggtgag
     2641 attctgaagg aacacacagc ccttacaccg tttgacgcta tcaagggcgt tgcagagggt
     2701 tctaaggcca tcagaatcac aacgggccaa ctggaactac tcaaagcaca gtccgcagtg
     2761 gcaggttgct caatgattca ggtgttgagt gccaaggttc atcaactggc ggataagctg
     2821 ggggtttaat gctatgcaca tttgattatc atgcgtttta tcgccataga ggtcaccttg
     2881 tccgtgagaa taacggaaca tggaaagatt taactcgtaa tgctgaaccc ttcacacacc
     2941 agcaattaaa atcccgcttc tccagatgga agaatgcacc agcagatact ggtgattttg
     3001 agattatcca taccttcgat tatgcctcag aagatgacct tcacgatgca attaagctga
     3061 ttgaagactt caatacctca gaagatgtgg atggtgacaa atataaatcc cgtggtaacc
     3121 gctataaccg tgatgatatc actcaggcca taaatgctgg tcaatctcag cgatacatta
     3181 cagaaaaata tggtatttca cggagacagt atttctacgt taagtcattg attaataaag
     3241 acaattaaaa ataaccatct aagaaacggt agaatgcact ttacaggcag agttgcacct
     3301 taatttgata aattacctta ccagccagtg caactccctc tgtaacccgc atggttactg
     3361 gcctcagaat ggctatttta agaggtctga ggtgtgtttc ataggagatt taaaaatgaa
     3421 taattaccca atgcaaatta tggttgataa cgacactgct atgatggtgc aaagcttcat
     3481 tgatgcaggt atcgcaatta attttgataa attactaaag ctgatggctg ataatgcaga
     3541 aaacatctct gactttattc agtcggtgga gtttaacgag cctcgaatga tgctaccaat
     3601 tacagacagc aacatgaaaa gattggttat cgagcaaacg aacatataca gtatttcccc
     3661 agagcaattt ttgaaaggtg ctgtgacaat cctgtacagt gataatattc tggtgacgga
     3721 ttctgtgagg gttcactaat gggtttaata attgcttgga cgttgcttgg cttatttgtt
     3781 ggttgcatta ttaacaatgc ggtaaataag aattgatgtt atcaacctgt taaaaagctg
     3841 ttgacaaata accttaagca tggcataatt ttaattatgg gagagcaatt ctctcttatt
     3901 tttctttcaa taatccgatg gatggatatt ctgagttgga aaggtgcaat gcttttcgtc
     3961 atggcctttc aggttagtct gagaggctat ctcaaaaagt actcttgttc attcattatt
     4021 gaatcctttg cccttcacag aaatgtttag ggctttttca gtttttaaaa gcacacttgg
     4081 caggttcttg ttcagtcaga ctcttcacct ctgattgctg agtgtgcttc tataaattga
     4141 aataacaaat aaactaaggg gtgaacgaat gattagaaat gaacatgaaa tgcttatcga
     4201 acacagacca agtgcacttg atattcacga attaactttg cgtgaatctt ttgatagagc
     4261 ttggcgggaa tcacacgaag taaataaagg tccatatatt ccaccagaac cactcgagat
     4321 tccaagagtt gaaattgatt tttctatgaa cgaacggtgt gaacttgatt tgaagttgaa
     4381 gctacaacgc cgatatttcc aagctattaa agactctcaa gaagcaatga gaagtgttta
     4441 cgagaaaatt cagtccaagc ttgaagacga aattgacgaa atggttattt cgatggagct
     4501 tcaaaccaat tcttatgctt gtttctctcg actagaccgt gacggttggg ggtatgaccc
     4561 tgtagagatt ggtaaagtga ttgcagattt accagaaggt caaagagttg ttcgtacaga
     4621 aagagctgtc agagcaccac atcgactgtc tctaattgtt gctgataaat cacaagctga
     4681 gcttgaagaa attgccaaag agaaactgat gaaagcaaga gaaaatgaga ttaatttgct
     4741 gaaatcaaga ctggctaagc tg
//
LOCUS       DAZPBM010000244         4704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPBM010000244 DAZPBM010000000
VERSION     DAZPBM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.247"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHUZQ_004038"
     CDS             complement(<1..737)
                     /locus_tag="ACHUZQ_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1027592.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUZQ_004039"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUZQ_004039"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1027593.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUZQ_004040"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUZQ_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1027594.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHUZQ_004041"
     CDS             4397..4678
                     /locus_tag="ACHUZQ_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1027595.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1130 a         1171 c         1182 g         1221 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg catt
//
LOCUS       DAZPBM010000245         4701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPBM010000245 DAZPBM010000000
VERSION     DAZPBM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.248"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            387..1469
                     /locus_tag="ACHUZQ_004042"
     CDS             387..1469
                     /locus_tag="ACHUZQ_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR1027596.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="ACHUZQ_004043"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="ACHUZQ_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR1027597.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="ACHUZQ_004044"
     CDS             complement(2127..2267)
                     /locus_tag="ACHUZQ_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR1027598.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="ACHUZQ_004045"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="ACHUZQ_004045"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR1027599.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="ACHUZQ_004046"
     CDS             complement(2623..2913)
                     /locus_tag="ACHUZQ_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1027600.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="ACHUZQ_004047"
     CDS             complement(2906..3076)
                     /locus_tag="ACHUZQ_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR1027601.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="ACHUZQ_004048"
     CDS             complement(3076..3531)
                     /locus_tag="ACHUZQ_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR1027602.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="ACHUZQ_004049"
     CDS             3796..4173
                     /locus_tag="ACHUZQ_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027603.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            4170..4598
                     /locus_tag="ACHUZQ_004050"
     CDS             4170..4598
                     /locus_tag="ACHUZQ_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027604.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT         1350 a         1049 c          980 g         1322 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagccaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcagtgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatggcactc
     4201 aagtcagtgg tgaagtaaga gatctagtaa ttcttcttga aactgatgga aaaacggtgt
     4261 ctgattattt gcagccaatc cctcccaaaa aagatacaca ctggagcatg attttcattc
     4321 cggtagcaat ttatatacta cttaatcttg tatacattat tgctcaccca ttatttcctg
     4381 aaaaatatct aaaaacatca acatctatat ttctattcat ttccttcata ttaagtgcaa
     4441 tagtagcata ttttgttttc cataagcata aatctgccac tctatccata tgcagcttag
     4501 tgttcttagt gttaattgta ggtgctaacg tagggtttat cagttacagc gacctaacac
     4561 aaaaagcgac gcaaaaatta gatagtttgt ctaaataatt atgttgccat gcgaagtatg
     4621 catggctgca tgtctgcctt ccatttaaaa tgggctaaga cctataaccc taaatattat
     4681 tctttattat cttctttacc a
//
LOCUS       DAZPBM010000246         4606 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPBM010000246 DAZPBM010000000
VERSION     DAZPBM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.249"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHUZQ_004051"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHUZQ_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUZQ_004052"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUZQ_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR1027605.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHUZQ_004053"
     CDS             complement(1327..3060)
                     /locus_tag="ACHUZQ_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1027606.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHUZQ_004054"
     CDS             complement(3057..3551)
                     /locus_tag="ACHUZQ_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1027607.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHUZQ_004055"
     CDS             complement(3673..4023)
                     /locus_tag="ACHUZQ_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1027608.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHUZQ_004056"
     CDS             complement(4066..4530)
                     /locus_tag="ACHUZQ_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1027609.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..>4606)
                     /locus_tag="ACHUZQ_004057"
     CDS             complement(4527..>4606)
                     /locus_tag="ACHUZQ_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1027610.1"
                     /translation="AGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT          995 a         1367 c         1154 g         1090 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcat
//
LOCUS       DAZPBM010000247         4481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPBM010000247 DAZPBM010000000
VERSION     DAZPBM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.250"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHUZQ_004058"
     CDS             complement(8..1069)
                     /locus_tag="ACHUZQ_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1027611.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHUZQ_004059"
     CDS             complement(1066..2463)
                     /locus_tag="ACHUZQ_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1027612.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHUZQ_004060"
     CDS             2618..3616
                     /locus_tag="ACHUZQ_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1027613.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHUZQ_004061"
     CDS             complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHUZQ_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR1027614.1"
                     /translation="EPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVH
                     YQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWL
                     SMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFST
                     QEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGL
                     SVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1227 c         1099 g         1063 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc g
//
LOCUS       DAZPBM010000248         4429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPBM010000248 DAZPBM010000000
VERSION     DAZPBM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.251"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(18..3122)
                     /gene="acrF"
                     /locus_tag="ACHUZQ_004062"
                     /pseudo
     CDS             complement(18..3122)
                     /gene="acrF"
                     /locus_tag="ACHUZQ_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3134..4291)
                     /gene="acrE"
                     /locus_tag="ACHUZQ_004063"
     CDS             complement(3134..4291)
                     /gene="acrE"
                     /locus_tag="ACHUZQ_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR1027615.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         1094 a         1178 c         1084 g         1073 t
ORIGIN      
        1 gcctttatgt ttctgattta tcctttaaag caacggcgga tcaccacaaa gaacaccggt
       61 acgaagaaga ttgccagcaa cgttgcagag accattcctc ccattacccc gatacccact
      121 gcgttctgcg cgccactgcc ggcaccgtta ctgatagcta gcggtaatac gccgagaata
      181 aaggcgagag aggtcatcag gataggacgc agacgcatac gtactgccat cagtgtcgct
      241 tcaacaacac ctttaccctt tttctccatg agatctttag cgaactcaac aatcaaaata
      301 gcgtttttgg ccgacaagcc aattgtcgtt agcaagccca ccataaagta gacgtcattt
      361 ttttgattaa agagtgttgc cgccagtagc acgccgacaa tccctaacgg cacgaccaac
      421 ataaccgaga caggaattga ccagctttca tagagtgcag caaggcacag gaagacaacc
      481 acaaaggaaa ttgctaccag agcgggagcc tggtttcccg ataagcgttc ctgatacgac
      541 atacctgtcc agtcataacc aatgcccgca ggtaattttg acgcaaggtt ttccatcaat
      601 gccatggcat cgccggaact ggttcctggc gcggcttccc cctgaatctc cattgacggc
      661 agaccgttgt agcgttccag tcgcggagag ccatacaccc aatgtgaagt ggtaaaggca
      721 gagaatggca ccatttcgcc gttggcgctg cggacataaa gtttatcgac atcttctggc
      781 agcatacgga atttggcatc cgcctgaaca tacacctttt tcacgcgacc acggtcgatg
      841 aagtcgttaa cgtaagtccc acccagcgcc gttgaaatgg tctgattgat gtcagaaagt
      901 gaaacaccta atgcctgcgc cttttcctgg tcaacttcca gtttaaactg cgcggtgtct
      961 tccagaccat taggacgcac gctgactaag ctggcaggat gttgcgccgc catgccaagc
     1021 aactggttac gggcctgggt cagggcatcg tgacccagcc cagcctgatc aattaactca
     1081 aagtcgaaac ccgttgccgt gcccagttca acaatggctg gcatattgaa tggaatgaca
     1141 aaaccgtcgc ggatcttgcc caattccatt ttggcacgat ggattaccgc ttccgcactg
     1201 ttttcgtcac cactacgctc ttcccacggt ttcagactga cgaaagccat accggcgttt
     1261 tgtgcctggc cgctgaagct aaagccgtta accgtaaaga cactttctac gttcgctttc
     1321 tcgttattca gatagtaatc cgtcacttga tccaacactt tttgcgtccg ctcttgcgtc
     1381 gcgccagcgg gtaactgaat catggtcaga aagacaccct gatcctcttc aggtaagaag
     1441 gaagacggaa gacgtaaaaa caacaccacc attcctgcaa caatcagcgc atagatcagt
     1501 aaatatcgtc ctgtggaacc gaggattttg ccgacgctgt tggtgtagtg gttaacgcta
     1561 tgatcgaagg tggtattaaa ccagccgaag aaaccgccct tattttcgtg atgctcagca
     1621 gagacgggtt taagcagcgt tgcacataac gcaggggtaa gaatcaatgc caccagaaca
     1681 gaaagcgcca ttgccgaaac gatggtgata gagaactggc gataaattgc cccagtagaa
     1741 ccgccgaaga atgccatcgg aataaatacc gctgacagca ccatcgcgat acccaccagt
     1801 gcgccctgaa tttgcgacat cgatttttcc gtcgcttctt ttggcgggag cttatcctcc
     1861 atcatcacgc gctcgacgtt ctccaccacc actatcgcat catcgacgag cagcccgatg
     1921 gcaagcacca tcccgaacat cgttagtgtg ttgatggagt aaccaaaagc ggcgaggatg
     1981 gcaaacgtcc ctaacaacac cacgggtacc gcaatggtgg ggatcagcgt tgctcgcata
     2041 ttctgcaaga acagatacat caccaggaac accagcataa tggcttcgaa cagcgttttt
     2101 accacttcgt gaatagaaag ctggacgaat ggcgtggtgt cataagggta gagaaccttc
     2161 attccctgcg ggaagaatgg ctgtaattcc gccagttttg ccttaatggc tttcgcggta
     2221 tcgagagcgt tcgcgccggt tgctagctta atccccaggc ccgccgccgg ttttccgttg
     2281 atacgagcga taacgttata gttttcaccg ccaagttcaa ctcgtgcgac atctttcagg
     2341 cgtaccaccg agccgtcact gtttacgcgc agggttactt tgccgaattc ttccggattt
     2401 tttaaccgcg tctgagcaat aatcgaggcg ttcaactgtt gccctggtaa cgctggcgtt
     2461 ccgcccaact gcccggcagc gatctgatcg ttctgtacct tcaactggtt aatcacatca
     2521 accggtgtca gtttatattt gtttagcaga tcggcgtcca gccagatacg catcgcatac
     2581 tgtgcgccga aaagctgtac gtcaccgacg ccattcagac ggctaagcgt atctttaacg
     2641 ttagaggcca catagtccga gatatcgtcc tgtgtggtgt ctgggttatc agagacaaag
     2701 cccgccacca tcaaatagct gctactggac ttttcaacac tgatcccctg ctgctgaacc
     2761 tcctgcggca gcaacggcgt ggcgagctgg agtttgttct gcacctgcac ttgcgcgata
     2821 tcaggatcgg tcccggactg gaaggtaagg gtaattgtca cgctaccggc ggaatcgctg
     2881 gtggaggaca tatacatcag gttatcgata ccattcatat tctgttcgat aacctgcgtc
     2941 accgtatcct gcacggtctg cgcatcagca cctggatagt ttgcctaaac agaaaccgca
     3001 ggcggtgcaa tggttggata ctgagcgacg ggcaattgta ggatcgccag tgcgcccgcc
     3061 atcatcagaa taatggccag cacccatgca aatatcggtc gtcgaataaa aaagtttgcc
     3121 atgtcagatt accttacttc gatgcagtat ctgcgggggt atcggtagtg gctttaacct
     3181 gctctcccgg acgcgctttt tgcaggccgc tgacaatgac ttgatcgcca gatttcagac
     3241 cttcgctaat caaccattta tcgccaatcg cctgactggc aacgacaggg cgcgcttcca
     3301 cctgactttt atcgttaaca atcagcacgg ttgcatcacc acgcggtgtg cggctaacgc
     3361 cttgttgcgg gataagaatg gcgtcaggtt ggacgccttc atcaatccgt gcacgcacaa
     3421 acatacccgg caaaagcgta tgttgcgggt tagggaagac agcacgtagg gttatggagc
     3481 cggtgcattc atcaacggtc acatcagaga attgcagcgt acctttcagg ggataggttt
     3541 gaccgttttc catgaccaac tctacgttgc tggtggcgtt ttccttatgc aaatttcctt
     3601 gctctacgga ttgcttcagc ctcataaaat cgttgctgga ttgggtcaca tcaacgtaga
     3661 taggatcgag ctgctggaca gtcgccagtt cagtcgtttg cccattagtg acaagagcgc
     3721 cttcggtcac ggtcgatttg ccgatacgtc cgctaattgg cgcagtcact ttggtataag
     3781 cgagattgat gcgagcgctt tcgactgtgg cttttgcggc aatcacggcg gcatcggcct
     3841 ggcgagcatc agcaatggcc tggtcgtact cctgctggct gatgtatttc gtacccacga
     3901 gcggaacgta acgttttacc gtcaaatgcg cgatggcggc ggcggcttca cttttcgcca
     3961 gttcgccttt cgcgctgtca taatttgcct gataggtcgc gggatcgatc tggtacaggg
     4021 actggcctgc ttgtacatcg ctgccttcag tgaaattacg attcagtacg atcccgctaa
     4081 cctgtgggcg aacttcggct atacggtaag cattggtgcg gcctggtaat tcagtcttaa
     4141 cttctaacgg ggccgttttc acaatatgaa cggtaacctg cggttcaccg acgtgagctt
     4201 tctcttctcc cttatcgtta caaccgacga ttaaagccgc ggagatcaga ataaaggagg
     4261 gcaggaggaa aaacctggca tgtttcgtca ttactattcc tcaaaaaaac aaaagcgcgt
     4321 tatttaccca gaaaggcaac gcgttaactc gcagaaagaa aaatacagtt tgctatccta
     4381 caaattatca ttcgtcgatg taaggaatag ttatgaatac tggcgtctc
//
LOCUS       DAZPBM010000249         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPBM010000249 DAZPBM010000000
VERSION     DAZPBM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.252"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUZQ_004064"
     CDS             complement(<1..105)
                     /locus_tag="ACHUZQ_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027616.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHUZQ_004065"
     CDS             complement(173..2122)
                     /locus_tag="ACHUZQ_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1027617.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHUZQ_004066"
     CDS             complement(2207..2542)
                     /locus_tag="ACHUZQ_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1027618.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHUZQ_004067"
     CDS             complement(2542..2898)
                     /locus_tag="ACHUZQ_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1027619.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHUZQ_004068"
     CDS             complement(2898..4394)
                     /locus_tag="ACHUZQ_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1027620.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZPBM010000250         4396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPBM010000250 DAZPBM010000000
VERSION     DAZPBM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.253"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHUZQ_004069"
     CDS             complement(<1..590)
                     /locus_tag="ACHUZQ_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1027621.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHUZQ_004070"
     CDS             complement(583..993)
                     /locus_tag="ACHUZQ_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR1027622.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHUZQ_004071"
     CDS             complement(999..1532)
                     /locus_tag="ACHUZQ_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1027623.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2586)
                     /locus_tag="ACHUZQ_004072"
     CDS             complement(1510..2586)
                     /locus_tag="ACHUZQ_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR1027624.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2583..3974)
                     /locus_tag="ACHUZQ_004073"
     CDS             complement(2583..3974)
                     /locus_tag="ACHUZQ_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR1027625.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4021..>4396)
                     /locus_tag="ACHUZQ_004074"
     CDS             complement(4021..>4396)
                     /locus_tag="ACHUZQ_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027626.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1052 a         1281 c         1039 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcgc
     2041 cgtcgccgct ttacctgttc cgggttttcc cagaaccagt cgcccgcatt catcgtcata
     2101 agccagcgtc tgaacctgcc ccagtaaccg gttaagacaa tcggcaacgg tttcgccgtg
     2161 ttccggctgg gcatcaatca ccgctgtctg cggcacgcca gcatcaacaa cggtgatgcc
     2221 aaatggcgca gccagttcac tgactattct gagcaggttt tttccgctct gctggagtgg
     2281 caaagcagag cagtcaacca gatcggctgt tttgcttcgc ccgacaattc ccatgctgac
     2341 gctgctggcg tcataacgaa gcggtagtgc ctccacatat ccggtgagca caggctcatc
     2401 cccgataagc acttcaacca gctcaccatt ttttatccgg ggttgataat cccggcttcc
     2461 gggccagcgg gtggtaatgg caacattaaa atcccgggca atacggttaa tgcccgcact
     2521 gatacggacg gatgtccagc cgccccattc gcgaccggaa acccgaagta aaacggtatc
     2581 attcatctga cgggtaccct taatgccctg accggaacaa agcccggatg agagatggca
     2641 tttcgatcca ggatatcagt ttcacgggac gcatcgtcgt accatgacgc agccagaaca
     2701 agcgcaggca gaacttccgc tggcgttcgc tctgcggttt cctccgtctg aaccagacgc
     2761 gcctgaatat cccggttcag ttctgtacgt aatgacgtca gctgaaaaaa cagcctgtca
     2821 tccgccgtac gcctgagctc ctgttcaatc gccgcgttca gcgattcgcg tatgatggtg
     2881 agattttccc gcgtgggtgg ttgcgctctc tcatcctgtc ctgtgctggc tgccacgctg
     2941 tcaagtgccg gatgagaaac gtgaataata tcggactgac gttcagtgga accaccaaca
     3001 gccacaaccg cctgctgatt tttcaccagg cttccgggtt gtggcagtga agtgaccgcc
     3061 cttgtggctt cgctgattgc cgtcgtccgg atgacagcag ccacaaggtt tgtctgctgt
     3121 ttttgcctga taaccgatgc ggaatctgtg ggccacaccg cacgcggtgc cagtcctggg
     3181 tccagcgtaa tgccggacat tgtcgttatg gactgcacca gatcctgtgt gttatccacc
     3241 agccgggttc cggcccgcca ggtatcctgc aacgtatgca caaaatcact ggcaaccgac
     3301 ggcggcatca gaatgacgga taaatcgccc tgcagtagtc tcgctccggc agaaacagcc
     3361 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
     3421 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
     3481 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
     3541 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
     3601 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
     3661 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
     3721 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
     3781 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
     3841 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
     3901 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
     3961 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
     4021 ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca ctcccgactc
     4081 gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta caaaaaactg
     4141 gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa aatcatcatc
     4201 tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg caaaatcaaa
     4261 tacacctata cttttgccga attcataaga agcttttctc aatataatcg caccgctggc
     4321 agaaggggat gggcacgata tcctcagttg tctgaatcca tctgtatcca agtcaacgtc
     4381 attatctgca gatgtt
//
LOCUS       DAZPBM010000251         4385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPBM010000251 DAZPBM010000000
VERSION     DAZPBM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.254"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..3511)
                     /locus_tag="ACHUZQ_004075"
     CDS             complement(<1..3511)
                     /locus_tag="ACHUZQ_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1027627.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4385)
                     /locus_tag="ACHUZQ_004076"
     CDS             complement(3963..>4385)
                     /locus_tag="ACHUZQ_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1027628.1"
                     /translation="ESANYIGARSVTAAIKPDIKLQPTRHVKTMDALQTGCVHINTVN
                     KALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKA
                     EHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1124 a         1126 c          906 g         1229 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatttaca
     4261 gtatttatat gaacgcatcc cgtttgcaag gcatccattg ttttgacatg acgggtaggt
     4321 tgcagcttta tatccggctt tattgcagcc gtaacgctgc gagcccctat gtaattcgcc
     4381 gactc
//
LOCUS       DAZPBM010000252         4364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPBM010000252 DAZPBM010000000
VERSION     DAZPBM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.255"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUZQ_004077"
     CDS             complement(<1..215)
                     /locus_tag="ACHUZQ_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1027629.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHUZQ_004078"
     tRNA            complement(330..405)
                     /locus_tag="ACHUZQ_004078"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHUZQ_004079"
     tRNA            complement(412..486)
                     /locus_tag="ACHUZQ_004079"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHUZQ_004080"
     tRNA            complement(603..687)
                     /locus_tag="ACHUZQ_004080"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHUZQ_004081"
     tRNA            complement(696..771)
                     /locus_tag="ACHUZQ_004081"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUZQ_004082"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUZQ_004082"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1027630.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUZQ_004083"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUZQ_004083"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1027631.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUZQ_004084"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUZQ_004084"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1027632.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHUZQ_004085"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHUZQ_004085"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAZPBM010000253         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPBM010000253 DAZPBM010000000
VERSION     DAZPBM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.256"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..583)
                     /locus_tag="ACHUZQ_004086"
     CDS             complement(<1..583)
                     /locus_tag="ACHUZQ_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1027633.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(646..1095)
                     /locus_tag="ACHUZQ_004087"
     CDS             complement(646..1095)
                     /locus_tag="ACHUZQ_004087"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1027634.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHUZQ_004088"
                     /pseudo
     CDS             complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHUZQ_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1044 t
ORIGIN      
        1 tgaagttcgg gatcatcgcg tcaacggtga tggcgtcagc aggctgtacg ccgccaacca
       61 gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc gttgaagtga
      121 cggaggtaat tggccccaac attttcgccc cgttggtgga aacccaggtg gagaaggcga
      181 acagcaccat actgataatg gtggtggcaa ccggcgtcat aatatattcg tatccgagga
      241 aggcatacga agcataagca ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta
      301 cgaaccagta ggtataggca gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc
      361 atgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg atgatcagac
      421 agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa
      481 gatcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga
      541 gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat ctccgaaaat
      601 gagtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt tgttatggaa
      661 ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat tgcagataaa
      721 ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct caacggtttc
      781 tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca ctacctgtaa
      841 tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat gcacttcaaa
      901 atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt
      961 caatgagtca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc
     1021 ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact gttctaagtt
     1081 atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg
     1141 aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta
     1201 aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc gctgccccag
     1261 gagttggagc cgaggccaag cagctggtga tcgaggttca gggtgatgtc atcactgcgc
     1321 tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct aggtatagtg ccaggcgctg
     1381 aagttaattg ggcgctgcgg aaccaccagc agaccgttac cgtggcggtt agtcagtgcc
     1441 gtccagcgga catgctgacg gttaccgttg ttctgcggga agggatagtt ctcgaacatg
     1501 gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc ggcgtagttt
     1561 tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat tcccatggtg
     1621 aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga aagcgccacg
     1681 ttaacctggc catcggcagc gatgcgccag atgtaggtgc agcgcattcc gaaatcaaac
     1741 accggcgggg caataaccgt gcggctgatg atcaacactt caccctcgct ctgttctacg
     1801 gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca caacccttcg
     1861 tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg ctcgcgggtc
     1921 agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt tgagaaggtg
     1981 atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga
     2041 cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag cgggaactga
     2101 taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta
     2161 atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg
     2221 ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc accttcggcg
     2281 cgcacctctg cgtgcagggt gtagtcatca agcgtggtaa accacagttt attttcgact
     2341 ttcaactcgc cgcgagtcag atcccgcgcg tggattttta ccggcgcgat aacctgtttg
     2401 tactctttca ggcccggtcc cggcgtctga tcggaataga tcaaaccatc aagacagaag
     2461 ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg
     2521 tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa
     2581 tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca
     2641 tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc
     2701 agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct
     2761 tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg cgtggtacat cgcgcggatg
     2821 ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc
     2881 tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg
     2941 gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc
     3001 atcacaaaca ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga
     3061 gcggtacgca cggagttgat gttgtgctgc ttcatcaact ggagatcttt ctcgacgcga
     3121 tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc
     3181 atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca
     3241 acgcgttgtg gtaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga
     3301 tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag
     3361 ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt
     3421 tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc
     3481 agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcacg
     3541 gtgaaatcgt taatatgtgt tagttgtttt ccgatcagat aaacatcgcg gaagatcccc
     3601 gctgaccacc acatatcctg gtcttccacg taggtagagt ccgcccattg catcacgcgc
     3661 acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg
     3721 cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg
     3781 acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata
     3841 cgttgatagg caccggttgg gttatcgctg ggaacaaacg gcacatcgat ggggaacgga
     3901 aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg
     3961 gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttcaggcac ttgcagcggg
     4021 tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct gctggtttcg
     4081 cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgtggcgca
     4141 agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga
     4201 gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa
     4261 atttttacta aaaagtggca ggtaaatgca tttactttaa ctggatcacg aaaataaacg
     4321 gcagcactgt
//
LOCUS       DAZPBM010000254         4309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPBM010000254 DAZPBM010000000
VERSION     DAZPBM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.257"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHUZQ_004089"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHUZQ_004089"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1027635.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHUZQ_004090"
     CDS             complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHUZQ_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1027636.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2269..2976
                     /locus_tag="ACHUZQ_004091"
     CDS             2269..2976
                     /locus_tag="ACHUZQ_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1027637.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="ACHUZQ_004092"
     tRNA            3082..3157
                     /locus_tag="ACHUZQ_004092"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="ACHUZQ_004093"
     CDS             3355..>4309
                     /locus_tag="ACHUZQ_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1027638.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1037 g         1017 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZPBM010000255         4291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPBM010000255 DAZPBM010000000
VERSION     DAZPBM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.258"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            374..982
                     /locus_tag="ACHUZQ_004094"
     CDS             374..982
                     /locus_tag="ACHUZQ_004094"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1027639.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1080..2471
                     /gene="selA"
                     /locus_tag="ACHUZQ_004095"
     CDS             1080..2471
                     /gene="selA"
                     /locus_tag="ACHUZQ_004095"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1027640.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2468..>4291
                     /gene="selB"
                     /locus_tag="ACHUZQ_004096"
     CDS             2468..>4291
                     /gene="selB"
                     /locus_tag="ACHUZQ_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1027641.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDA"
BASE COUNT          954 a         1109 c         1265 g          963 t
ORIGIN      
        1 ccggatggcc ggacgtcact ccgccggggc acaccgaaca ggccacaccg gtgggggcac
       61 cgatgcgcac cccggctgaa ccctgaacaa tgctaccgcc atactgcgtc atgtcgccct
      121 gacgcgctgc cggttttccg ctcatctgct catccttgtt ttatcgggtt atttgtctgc
      181 ttccaggcaa aaccctacag atttataaga aagccttaag tgagatatcc atcagataga
      241 aacaattagg aaaatattta tatcaccatg ataaatatgt gatttatttg gaagtaacaa
      301 accatcaaca ttttctgccc tttttcccat caccaggcca tactgatatt tttcctcgca
      361 caaaggactc ggcatgaaac tcgtcggtag ctacaccagc ccgtttgtac gcaaactttc
      421 tattctgttg ttagaaaagg gcataacttt cgaatttatt aatgaactgc cctataacgc
      481 ggacaacggc gtggcgcaat ttaacccgtt aggaaaagtg ccggtgctgg tgaccgaaga
      541 gggcgaatgc tggtttgatt cgccgatcat cgctgaatat attgagttaa tgaatgtcgc
      601 tccggcgatg ttgccgcgcg atccgctgga gtcgttgcgg gtgcgcaaaa ttgaggcgct
      661 ggcggatggc attatggatg ccgggctggt atcggtgcgt gaacaggcgc gtccggcggc
      721 gcagcagtct gaagatgaat tgttacgcca gcgggagaaa atcaaccgca gtctggatgt
      781 gctggaagga tatctggtcg atggcacact caaaaccgat acggtcaatc tggcgactat
      841 cgccattgcc tgtgctgtcg gatatctcaa tttccgccgc gttgcgccag gttggtgtgt
      901 cgaccgccca cacttagtca aactggtcga aaacctgttt agccgcgaaa gttttgctcg
      961 caccgaaccg ccaaaggctt gatgcgcgat atgtcctcct gacccatctc acgttacaat
     1021 ccgtggttat gttaaacgcc cttctccgtg tgagagggcc ttgatcagcc aggtttccta
     1081 tgacaaccga aacgcgttcc ctctatagtc aacttccggc tattgatcgc ttattgcgcg
     1141 atagctcctt cctttctttg cgtgatactt atggtcacac ccgcgtggtg gaattgttgc
     1201 gtcagatgct cgacgaagcg cgagaagtga ttcgtgacag ccagacgctt cctgcgtggt
     1261 gtgaaaactg ggcgcaagaa gtcgatgccc ggttgacgaa agaggcgcag agcgcgctgc
     1321 gtccggtgat caacctgacg ggaaccgtgc tgcataccaa ccttggacga gctttacagg
     1381 cggaagccgc ggtggaagcc gttgcgcagg ctatgcgttc gccagtgacc ctcgagtacg
     1441 atctggacga cgccggacgc ggacatcgcg atcgggcgct ggcgcaactg ctgtgccgta
     1501 ttacgggggc ggaagatgcc tgtatcgtca ataacaatgc ggcggcggtg ttattgatgt
     1561 tggcggccac tgccagcggg aaagaggtgg tagtatctcg cggcgaattg gtggagattg
     1621 gcggcgcgtt tcgtattccc gatgttatgc gtcaggcagg ctgcacccta cacgaagtag
     1681 ggaccaccaa ccgcacgcac gcgaatgatt atcgtcaggc ggtgaatgaa aataccgcac
     1741 tgttgatgaa agtacatacc agtaactaca gcattcaggg gttcaccaaa gcgatagatg
     1801 aagcggaact ggtggcgctc ggcaaagagc tggatgttcc cgtagtgact gatttaggca
     1861 gtggctcgct ggtcgatctt agccagtacg gtttgccgaa agagccaatg ccgcaggagt
     1921 tgattgcggc gggcgtcagt ctggtgagct tctccggcga caagttgtta ggcgggccgc
     1981 aggcagggat tatcgttggt aaaaaagaga taatcgcccg cctgcaaagc cacccgctga
     2041 agcgtgcatt acgcgcggat aaaatgaccc tcgcggcgct ggaagccacg ttgcgtcttt
     2101 atttacaccc tgaagctctg agtgaaaaat taccgaccct gcgcctgctt acccgcagcg
     2161 cagaggtcat tcaaatccag gcacaacgtt tacaggctcc ccttgccgca cattacggcg
     2221 cggagtttgc ggtacaggtt atgccatgtc tttcgcagat tggcagtggt tcgctgccgg
     2281 ttgatcgcct gccgagcgcg gcattaacgt ttacacccca tgatggacgc ggtagccacc
     2341 ttgagtcatt agccgcccgc tggcgtgaat tgccagtgcc gatgattggt cgtatttatg
     2401 acggacgatt gtggctggat ttacgctgcc ttgaagatga gcaacggttt ttggagatgt
     2461 tgttgaaatg attattgcga ctgccggaca cgttgaccac ggcaaaacaa ccttattgca
     2521 ggtgattact ggcgtaaatg ctgaccgtct gccggaagaa aaaaagcgcg gcatgaccat
     2581 cgatctcggc tatgcctact ggccgcagcc ggatggtcgc gtgcctagtt ttatcgacgt
     2641 acccggtcat gaaaagtttc tttccaacat gctggcgggc gttggtggta tcgatcacgc
     2701 gctgttggtg gtggcgtgcg atgacggcgt gatggcgcag acccgtgagc atctggcgat
     2761 tttgcagctg accggtaacc cgatgctgac agtggcgctg accaaagccg atcgcgtgga
     2821 cgaagcgcgt gttgatgagg ttgaacgcca ggtaaaggag gtgctgcggg aatacggttt
     2881 tgctgaggca aaactgttta tcaccgcagc aaccgaaggt cggggaattg atgccctgcg
     2941 cgagcatctg cttcagttac caggacgtga acacgccagc caacatagtg tccgcctcgc
     3001 gatcgaccgc gcatttaccg taaaaggtgc cgggctggtc gtcaccggta cggcgttaag
     3061 cggggaagtg aaggtaggcg attcactctg gctgactggt gtaaataaac cgatgcgtgt
     3121 acgtgcgctg catgcacaaa accagccaac agaaaccgcc catgccgggc agcgtatcgc
     3181 gcttaacatc gcgggtgatg cggaaaaaga gcagattaac cgtggcgact ggctgcttgc
     3241 cgatgcgccg ccagagccgt tcacacgggt gattgtcgag cttcaaaccc atacgccgct
     3301 gacccagtgg cagccgctgc atattcacca cgccgccagc cacgttacgg gacgcgtttc
     3361 gctgctggaa gataaccttg ccgagctggt gttcgacacg ccgctgtggc tggcggataa
     3421 cgatcgctta gtgctgcgcg atatctcggc ccgcaacacg ctggcggggg cgcgtgtggt
     3481 gatgcttaac ccgccgcgtc gcggtaaacg taagccggaa tatctgcaat ggctggcgtc
     3541 tcttgcacgg gcgcagagcg atgccgatgc gttatctgtt catctggaac gcggcgcggt
     3601 taaccttgcg gatttcgcct gggcgcgcca gctcaacggc gaagggatgc gcgaattgct
     3661 gcaacagcct ggctatattc aggctggtta tagcttgttg aatgcgccgg ttgccgcccg
     3721 ctggcagcgg aaaattctcg acacattagc gacttatcat gagcaacatc gcgatgaacc
     3781 tggtcctggg cgcgaacgtc tgcgacgtat ggcgttgcca atggaagatg aagcgctggt
     3841 actgttgctg attgaaaaga tgcgcgaaag cggcgacatc ctcagtcatc acggttggct
     3901 gcatctgccg gatcacaaag cgggcttcag cgaagagcag caggccatct ggcaaaaagc
     3961 agagccgctg tttggtgacg agccgtggtg ggtgcgtgat ctggcaaaag agacgggtac
     4021 cgacgagcag gcaatgcgcc tgacgctacg ccaggcggcg cagcaaggaa taattaccgc
     4081 gatcgtcaaa gatcgttatt accgtaacga tcggattgtc gaatttgcca atatgatccg
     4141 cgatctcgat caggagtgtg gttcaacctg cgcggcggat ttccgcgatc gcttaggcgt
     4201 aggccgaaag ctggcaattc agattctgga atattttgac cgcattggct ttacgcgtcg
     4261 tcgtggaaat gatcatttat tgcgcgacgc a
//
LOCUS       DAZPBM010000256         4201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPBM010000256 DAZPBM010000000
VERSION     DAZPBM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.259"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..747
                     /gene="ydeO"
                     /locus_tag="ACHUZQ_004097"
     CDS             <1..747
                     /gene="ydeO"
                     /locus_tag="ACHUZQ_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1027642.1"
                     /translation="SVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFK
                     EIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFS
                     ELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISES
                     LLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHF
                     GNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            1148..2830
                     /locus_tag="ACHUZQ_004098"
     CDS             1148..2830
                     /locus_tag="ACHUZQ_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1027643.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2882..4039
                     /locus_tag="ACHUZQ_004099"
     CDS             2882..4039
                     /locus_tag="ACHUZQ_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1027644.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4005..4127)
                     /locus_tag="ACHUZQ_004100"
     CDS             complement(4005..4127)
                     /locus_tag="ACHUZQ_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027645.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
BASE COUNT         1282 a          875 c          827 g         1217 t
ORIGIN      
        1 tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc
       61 tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag
      121 cggcatttta aagagatcgg atttactgaa aataccatta aaaaatatct acaatgcact
      181 aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg
      241 actggattgc ttaatgaaat gattgcttat cttaactcgg aagaacgcaa tcatcataat
      301 ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa aggtttcatt
      361 acactattaa ctaacggtgt gctatccgtt tccggaaaag tgagaaatat tgtcaacatg
      421 aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat cagtgaaagc
      481 ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct attagatgcc
      541 agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa
      601 caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac
      661 agtccgaaga gagtttctaa ggagtaccgt tgtcaaagtc acatgggtat gaatacgggc
      721 aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgct
      781 gcgtaagaat taaatggggc agccattccc tgccccgcgt tgtttttagg cgatatattt
      841 attgaaataa ataagtgaca tccatcacat atttatgcac ttgcataacc tgttgcagat
      901 tatttatgat ctcaattcgg cattttgtca gtaaaatgca ataatttatt aaatatcaat
      961 aaattagttg tttatcggcg agaaattact taatagaaca gaaagtaatg tcaacgcttt
     1021 atggactgtt atttcccttt ttttagctaa atctgctatc tctttatgtg actaacttca
     1081 cttacatcca cttatttctc ttcgtaaaat tacttcggaa ttaagtacaa taagaagagg
     1141 aacatttatg aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact
     1201 ggcatctggt atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac
     1261 caaaacaaac gttgctttct cagattttac gccgactgaa tacagtacca aaggaaagcc
     1321 aaatattatc gtactaacca tggatgatct tggttatgga caacttcctt ttgataaggg
     1381 atcttttgac ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat
     1441 agataaagcc attgaagctg cacaaaaatc aacgccgacg ctcctttcat taatggatga
     1501 aggcgtacgt tttactaacg gctatgtggc acacggtgtt tccggcccct cccgtgccgc
     1561 aataatgacc ggtcgagctc ccgcccgctt tggtgtctat tccaataccg atgctcagga
     1621 tggtattccg ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac
     1681 tgcagcagta ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa
     1741 acaaacgcgt gactatcatg acaacttcac cacattttct gcggaagaat ggcaacctca
     1801 aaaccgtggc tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc
     1861 cccttcactg ttcaaaaatc gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt
     1921 aaccgatgag gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct
     1981 ttacctggct tataatgctc cgcacctacc aaatgataat cctgcaccgg atcaatatca
     2041 gaagcaattt aataccggta gtcaaacagc agataactac tacgcttccg tttattctgt
     2101 tgatcagggt gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac
     2161 aattattctc tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg
     2221 ggcgcaaaaa ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat
     2281 gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt
     2341 ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg
     2401 tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gcgccacata aaaatctgac
     2461 ctggataacc tcttattctc actggtttga tgaggaaaat attccattct gggataatta
     2521 ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca ctgaggactt
     2581 aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa
     2641 caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc ttgccgccgc
     2701 caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca
     2761 gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga
     2821 agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga gcgagatagc
     2881 gatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt
     2941 ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa
     3001 agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta cctggcaagg
     3061 cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact atcaacaacg
     3121 ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt tattgaataa
     3181 tgaatggtgt gcctttctca aagaacatga atttctggtt ggtatctcga tcgatggccc
     3241 ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt ttgcaaaagt
     3301 gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt taaccgtcat
     3361 taataacgtt aatgtccatt accctcttga ggtttatcat tttttaaaat ctatcggcag
     3421 taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg atttcagtgg
     3481 tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa
     3541 gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg
     3601 tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt gtattttcca
     3661 ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg aatgcgacca
     3721 ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca aaacgatgaa
     3781 cagtgtacaa ctgacagcgc aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata
     3841 taaacctatc tgcaatggcg gttgtcctaa gcatcgtatt actaaagtaa acaatgagac
     3901 tgtttcttat ttttgcgaag gttataaaat ccttttttca accatggtac cttatatgaa
     3961 cgccatggtt gagttagcta agaacagagt accgctttac cacattatgg atgttgcaaa
     4021 acaaatggag aataattaat aatgctctct tttaactcca ttcttagcct ccttcttatg
     4081 accttaacaa ggcacccgac ataaattcct gtcgggtgtt tcgtcatcat catcttccct
     4141 tgcgaaaatc ctggcttacg tggtatgcgt cagggcatta gcaataccct tgattatcaa
     4201 t
//
LOCUS       DAZPBM010000257         4159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPBM010000257 DAZPBM010000000
VERSION     DAZPBM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.260"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            121..1224
                     /gene="proB"
                     /locus_tag="ACHUZQ_004101"
     CDS             121..1224
                     /gene="proB"
                     /locus_tag="ACHUZQ_004101"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1027646.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1236..2489
                     /gene="proA"
                     /locus_tag="ACHUZQ_004102"
     CDS             1236..2489
                     /gene="proA"
                     /locus_tag="ACHUZQ_004102"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1027647.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2604..2679
                     /locus_tag="ACHUZQ_004103"
     tRNA            2604..2679
                     /locus_tag="ACHUZQ_004103"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2637..2639,aa:Thr,seq:cgt)
     gene            3285..3443
                     /locus_tag="ACHUZQ_004104"
     CDS             3285..3443
                     /locus_tag="ACHUZQ_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027648.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         1096 a          962 c         1070 g         1031 t
ORIGIN      
        1 gcttgctgat cccgcgcaac aaaacgccat gctttgctcg cagatggttg gcaaccgacg
       61 acagtcctgc taaaacgttc gtttgatatc atttttccta aaattgaatg gcagagaatc
      121 atgagtgaca gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg
      181 cgccgtctga accgtgccca tattgttgaa cttgttcgcc agtgcgcgca gttacatgcc
      241 gccgggcatc ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg tgagcacctg
      301 ggttacccgg aactgccagc gactatcgcc tcgaaacaac tgctggcagc ggtagggcag
      361 agtcgactga ttcaactgtg ggaacagctg ttttcgattt atggcattca cgtcgggcaa
      421 atgctgctga cccgtgctga tatggaagac cgtgaacgct tcctgaacgc ccgcgacacc
      481 ctgcgtgcgt tgctcgataa caatatcgtt ccggtaatca atgagaacga tgctgtcgct
      541 acggcagaga ttaaagtcgg cgataacgat aacctttctg cgctggcggc gattctggcg
      601 ggtgccgata aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgacccgcgc
      661 agcaatccgc aggcagaact gattaaagat gtttacggca ttgatgacgc actgcgcgcg
      721 attgccggtg acagcgtttc aggcctcgga actggcggca tgagtaccaa attgcaggcc
      781 gctgacgtgg cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc
      841 gttattggtg atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact
      901 ccgcttgaaa accgtaaacg ctggattttc ggtgcgccgc cggcgggtga aatcacggta
      961 gatgaagggg caactgccgc cattctggaa cgcggcagct ccctgttgcc gaaaggcatt
     1021 aaaagcgtga ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa cctcgaaggc
     1081 cgcgatatcg cccacggcgt cagtcgttac aacagcgatg cattacgccg tattgccgga
     1141 caccactcgc aagaaattga tgcaatactg ggatatgaat acggcccggt tgccgttcac
     1201 cgtgatgaca tgattacccg ttaaggagct ggccgatgct ggaacaaatg ggcattgctg
     1261 cgaagcaagc ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat cgcgtgctgg
     1321 aaaaaatcgc cgatgaactg gaagcacaaa gcgaaatcat cctcaacgct aacgcccagg
     1381 atgttgctga cgcgcgagcc aatggcctta gcgaagcgat gcttgaccgt ctggcactga
     1441 cgcccgcacg gctgaaaggc attgccgacg atgtacgtca ggtgtgcaac ctcgccgatc
     1501 cggtggggca ggtaatcgat ggcggcgtac tggacagcgg cctgcgtctt gagcgtcgtc
     1561 gcgtaccgct gggggttatt ggcgtgattt atgaagcgcg cccgaacgtg acggttgatg
     1621 tcgcttcgct gtgcctgaaa accggtaatg cggtgatcct gcgcggtggc aaagaaacgt
     1681 gtcgcactaa cgctgcaacg gtggcggtga ttcaggacgc cctgaaatcc tgcggcttac
     1741 cggcgggtgc cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt gaaatgctgc
     1801 gtatggataa atacatcgac atgctgatcc cgcgtggtgg cgctggtttg cataaactgt
     1861 gccgtgaaca gtcgacaatc ccggtgatca caggtggtat aggcgtatgc catatttacg
     1921 ttgatgaaag tgtagagatc gctgaagcat taaaagtgat cgtcaacgcg aaaactcagc
     1981 gtccgagcac atgtaatacg gttgaaacgt tgctggtgaa taaaaacatc gccgatagct
     2041 tcctgcccgc attaagcaaa caaatggcgg aaagcggcgt gacattacac gcagatgcag
     2101 ctgcactggc gcagttgcag gcaggccctg cgaaggtggt tgctgttaaa gccgaagagt
     2161 atgacgatga gtttctgtca ttagatttga acgtcaaaat cgtcagcgat cttgacgatg
     2221 ccatcgccca tattcgtgaa cacggcacac aacactccga tgcgatcctg acccgcgata
     2281 tgcgcaacgc ccagcgtttt gttaacgaag tggattcgtc cgctgtttac gttaacgcct
     2341 ctacgcgttt taccgacggc ggccagtttg gtctgggtgc ggaagtggcg gtaagcacac
     2401 aaaaactcca cgcgcgtggc ccaatggggc tggaagcact gaccacttac aagtggatcg
     2461 gcattggtga ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa gtagccattt
     2521 gattcacaag gccattgacg catcgcccgg ttagttttaa ccttgtccac cgtgattcac
     2581 gttcgtgaac atgtcctttc agggccgata tagctcagtt ggtagagcag cgcattcgta
     2641 atgcgaaggt cgtaggttcg actcctatta tcggcaccat taagaaaatt aataacttag
     2701 cttaacttct ctaagaaatt gtatcttctg ttatcactta caaacgccta tacgttgtta
     2761 tgggctttgg tagtgtaaca cgtatgggcg agtagttccc aggctcaacc tcgtaacaag
     2821 ttcctttgaa gctggaagac gtgccaccgt ctctaaacct tctaccaaaa ctcttagcaa
     2881 gatgttccac acctggcgcg gtgtccaatg atgctacacg tcgtgagacg ttgcagagga
     2941 taaacaggct aaatgatggg aagagtttta agtgcaatag tggattaaag cccacctctt
     3001 caaaacctaa cttgatcggg aaagaatcaa gctctagcac ggtttcgtcc accggagtac
     3061 aacgggtgcc tgataagaga agaactgcta agacttcata gcttttgtga aaaagccctt
     3121 tttagtggat acgcctacgg cgcttatttt ttggtataac catcaaaggc gctttatggc
     3181 tcctattgtc ttgaaaatgt atctttactt gtattgtgtt ttatgcgtag tctccctgtg
     3241 tagtatgtaa cttgttaatt ttcatatgaa tacagaggca cacgatgaaa aacattgcag
     3301 caatcaaacg taataaccgc aagattcacg ctcgtaagtt cctgtctacg ccagaaggaa
     3361 aagcctggct agagcgtaaa cagagagaga acgaagaaag aaaactcctt agtgagttga
     3421 aatggcttag agaggatttc tgaaaatctc cagaacgcaa caggacgcat tctaacgctg
     3481 ttttcattac tcggtatagg gataggtgat gtgtgtttaa tgcgtctgtg cgcttgtttt
     3541 gtgccttatt ttgcattttc ataatgcgga agagaacacg gaaaacctta aaattaatta
     3601 ttggggtatt taaggctatt ttcaggtgat ttaagggtat taaacttgat taaaatataa
     3661 acagataatt atgtatctta ttgatttata aggttatttt taagtaaagg caaataaaaa
     3721 ataaatagcc ataaatagct ataaatacat cttaatacct atatcccttc ccttatggct
     3781 agattgcgag aggtgcttaa cgcgattttc agcgcccccc gatctggaaa atccagaatc
     3841 cccaaatatc agcaaggaat tttcttgcgt aatcaacatc gtttttacct attaaatcat
     3901 tacacagaaa gcctttctgt aatgtctctc actaaacttt cactaaacac gagaatgatc
     3961 ggttttatgt cggatcagat gctagaaaca gcaccacgtc tcacaagggc tgtaagcgat
     4021 gaaaccagtg tttatgcggg tgcaggtcaa aatataggcc aaaatccact taacataata
     4081 aatgttatat gtgctaagga acacatataa cgccttgaat tgatgtatca ggggaaaagt
     4141 gatggatttt ttacgtagg
//
LOCUS       DAZPBM010000258         4075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPBM010000258 DAZPBM010000000
VERSION     DAZPBM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.261"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUZQ_004105"
     CDS             <1..784
                     /locus_tag="ACHUZQ_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1027649.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1756
                     /locus_tag="ACHUZQ_004106"
     CDS             797..1756
                     /locus_tag="ACHUZQ_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1027650.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHUZQ_004107"
     CDS             complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHUZQ_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1027651.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHUZQ_004108"
     CDS             complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHUZQ_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1027652.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1224 a          952 c          861 g         1038 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1141 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1201 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1261 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1321 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1381 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1441 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     1501 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     1561 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     1621 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     1681 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     1741 ggttatgtac aactaatcta tctcccccac cttaactttg ggggaggtgc cgattaacgc
     1801 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     1861 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     1921 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     1981 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2041 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2101 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacga tttttctggt
     2161 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2221 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2281 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2341 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccacg
     2401 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     2461 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     2521 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     2581 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     2641 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     2701 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     2761 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     2821 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     2881 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     2941 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3001 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3061 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     3121 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caatacatgc tccagtcgct
     3181 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3241 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaacaagaa
     3301 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3361 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3421 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     3481 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     3541 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     3601 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     3661 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     3721 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     3781 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     3841 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     3901 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     3961 actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4021 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacgg
//
LOCUS       DAZPBM010000259         4072 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPBM010000259 DAZPBM010000000
VERSION     DAZPBM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.262"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            75..776
                     /locus_tag="ACHUZQ_004109"
     CDS             75..776
                     /locus_tag="ACHUZQ_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1027653.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPD
                     EQKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRA
                     QYFKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTG
                     DRTIAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVA
                     LHFRFKEDKQIKMTI"
     gene            complement(1034..3181)
                     /gene="mobQ"
                     /locus_tag="ACHUZQ_004110"
     CDS             complement(1034..3181)
                     /gene="mobQ"
                     /locus_tag="ACHUZQ_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR1027654.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEAL
                     PKDSNSAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRI
                     PLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGL
                     AALRAQLLKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGN
                     GEMTCYLLLHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQR
                     VYSSATFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQG
                     RLPPTLMPVMSSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVI
                     SALTDKGLRYAGYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLF
                     RSLGFNRTWEEWQREEDARTHTTERRHDRGWSQ"
     gene            3360..3614
                     /gene="mobC"
                     /locus_tag="ACHUZQ_004111"
     CDS             3360..3614
                     /gene="mobC"
                     /locus_tag="ACHUZQ_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR1027655.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
BASE COUNT          962 a         1030 c         1066 g         1014 t
ORIGIN      
        1 aaaaaaaaaa cttccaccca tatgataagt ggcgtacagt aaaaaaacgc caccaatagc
       61 aacgagcaag cgaaatgtca gatctgatag cttacaaatc aaacgccctt atcgaggcaa
      121 gctacaagct cactttgcag gaacagcgct tcctgctggt ctgtatcagc cgcctgaaat
      181 ccggagaaga ttccccttcg ccggatgaac aaaaaaccat gaccatcacg gctgcagagt
      241 ttcatgatgc ctttcctgac atgggacggc agcacgctga agctaggctc aaggaagcga
      301 tagatcgcct ctgggacagg tcagtaatca tcaagaacga ggaaaaacga gaagagttcc
      361 gctgggtgca gtaccgggcg cagtatttca aaggtgaggc gaaagtagat atcaccttct
      421 ccgatgccat catgccgtat ctgacacagc tcaaggggca gtttacgcgg gtagtcgtga
      481 agaacgtctc cagcctttcc agcacctaca gcatccgcat ttacgagctg ctccagcagt
      541 tccgcagcac cggtgacaga acgatagcta tcgatgattt ccgaaccatg cttgccgttg
      601 aagataaata tcaacaattc aaagacttga ataagatgat aatcaagcca gccatcgccg
      661 aactgaacga aaaaagcgat ctggttgtaa ctgttgagac gatcaaaaag gggcgcacgg
      721 ttgtagccct gcattttcgc ttcaaggaag acaaacagat caagatgacg atctaaaatc
      781 gattttaagg cacctaaaac gctccctgct gcgttttttt tgtgttagta gtgcgaaggt
      841 actattgaag tgaaaaattg cgtcaaaaac gctctgatgc agcgaaaaaa gaccccccaa
      901 tttttggggg cggaaaaaca aaactatgct tgcgttatgt gagtgtgctt cttgcattca
      961 tccctgtcga tgaacatcga agaacatcga cgaacataac acgatgttaa acaaaacaaa
     1021 atattgttca cattcactgt gaccaaccgc gatcatgtct cctctcggtg gtgtgtgttc
     1081 gtgcatcttc ctcgcgctgc cattcctccc aggtacgatt aaaacccagc gagcggaaca
     1141 gatgatccgg ctgtttttct ggtgtgttga cggcagcaaa aagcggtgcc tcacgggttt
     1201 tcagatgaaa ctgcatcagt tctggtgcag gtgatatgtg gctgtctgca tcgtgattta
     1261 cggcaacacc ataccctgca taccgtaacc ccttatcggt cagcgcactg atgaccagat
     1321 gcctgttatc ggctgaggga acggcaaacg cgggatacag cgtaccatta agccggataa
     1381 caccgcccag agagtcatcc acgcccatga ccagcaaaag ctgaagctgg cgggtttccc
     1441 ggtcgacaga actcatgacc ggcatcagcg tcggtggcag acgtccctgc ataaaatcca
     1501 ccttccttgc cgctggtgat ggcatggctg gctggcggtc ttctccaaga cagaaagact
     1561 ccggccatgt cttcggctgg cggagattgc gggtatgttg cggccatgtc tggcaaacct
     1621 gcgtcagcag ttgctcaaag gttgcagagg aataaacacg ttgcagtggc ggacgttggc
     1681 cgatttcgct tcggtcacag atataaccac tcctgtcacg cattaaatca acactctcaa
     1741 agattctggt gcggtatttc tccagctcgt tcccccagag cgtatattct ccgttccggt
     1801 tgtgaagcag aaggtaacag gtcatttccc cgttaccagg catgggagcc tcgccatacc
     1861 ccgtaacgac tgctctcagc acccctgaat ccataacggc attgtcattt ttcctgagct
     1921 gctccgcgcg actacgttca gtatcttcct gttccttccg gcgcaattcc tcttcacgtt
     1981 taagcaactg ggctctgaga gctgcaaggc cggattcccc ccactgctgc cactgttttt
     2041 caaatgccat ctgactcgcg ggaatgtcct gtttctccac cttccggtaa tgctgtcgta
     2101 atttttccat ttgctggctc agagactgga tttttccctg ctcactacgg tattccggtg
     2161 ccgcccattg catcagggga atacgggaaa aaccggacga aaacggcgat gattttttca
     2221 ggctggcagc atggctcatc gcttcctttc gttcggtgtt cagctcttcc cagcgtctgc
     2281 gcgtcgcctg catatctgcc atcatcgaca tggcattact ggtgagtttc catgccgagt
     2341 tactgtcctt cggcaacgct tcgataatgt cacgtagtgt gtacgaagtt gcgttgtcag
     2401 gaagcccgat attcagaagg tcagccacac ggcgggcagg ctctgtcagc cctccgttcc
     2461 tgagttcggt gcgggaaatc tgtatttcca ggttaagcct gttaatcaga cgattctgtt
     2521 cgccgcgttc tgtttcgata ccgcgtgttt ccatcgcaca ggcagacttt ccgaggtgaa
     2581 taccaggcgt tttctcaagc ccctgtcgct cgtatgaacg atggtctatc tcttcctgat
     2641 aaccggcgtt cgccagtgcc ctgtttgcat ggtcagccca tgatgcccgc cacgtctcgg
     2701 catgtgtccg gtcgttccag tccctgacct ttccgctgaa cccagcaggt ccgatagccc
     2761 tcgtggtcag catgatgtgc gcatggggat ttgttttatc catgtgatgg aacgccacat
     2821 cagcaatcat gccctgactg acaaagtttt ctctgacaaa ggcgagaacg gtttcccgtg
     2881 cggcatcctg cggcagttcg tgggggatgg cgagttcaat ttcccgcgca agttgtgagt
     2941 tcttccggcg ttcggccttc tcaacggcat tccagagttc ggcacggttc gtacaccagg
     3001 caggggcggt ggcgggggtc agaatcacag cctcagccac accgcttttg cgggtgtagt
     3061 cgtgagtcag tccggtacgc tcatcaagca tcagtgaacc ggaacgatag gcagcggaag
     3121 ctacggcgct gtagccgctg cttctggaga taattttcat gctgagatga taaatcgcca
     3181 tttctgccgt aatgcctttt tgtagttttc tttcagcccc gcagggcgca cacaatcgaa
     3241 acggagtgga gattgtataa gtgcgcactt cgtgatttgc cgtgcattat gccagctcag
     3301 tttttaaact accagtgacg cgctaaaatg cgatttttca tcgcgcacgg agttacatca
     3361 tgagcagaac gctcgaacag aagattgctg atgctgaggc gagattacag cgcctgaagg
     3421 cgaagagccg gagcctggac acagcgcaga aggttgttgt gggtgctgca ttgctggcaa
     3481 aggtcagaaa gccggaggag gtgcagttgc gtgcctggtt actccagttc ctgaaggcag
     3541 aggtgacacg acaggctgat gtgacgcgca tactgccgct gattaacgag ctggaggcgc
     3601 tgccagaaca gtgattgtat cgtccgctcg ccgcagcccc agcgaccgag cgttagcgag
     3661 cgagtgggcg aggaagcgga agtgaatcag tcgccaggtt gagcacatac gcacggtttt
     3721 tcggcaggtg atatgcgctg aacccgcatg aatactgggt ttccacaggg ttggaggggg
     3781 tgtattcgaa acttgcaatt ctgctttatt tcaacgcatt gtttttattg gttttttgtt
     3841 ttcagcaccc caagttatcc acaggctcgc cgttaagttt ttaagatctt atttttagat
     3901 ctttttttta aaaccttttt aggtcttgta acaccatgat tttaaagatg ttctggcaag
     3961 ataagtggag cttttggggg tgaaaggtgg agcttttggg ggtgaaaggt ggagcttttg
     4021 ggggtgaaag gtggagcttt tgggggtgta atccacagaa aaagggggtt gc
//
LOCUS       DAZPBM010000260         4048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPBM010000260 DAZPBM010000000
VERSION     DAZPBM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.263"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHUZQ_004112"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHUZQ_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1027656.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUZQ_004113"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUZQ_004113"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1027657.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUZQ_004114"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUZQ_004114"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1027658.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUZQ_004115"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUZQ_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1027659.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1142 c          972 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacccga
//
LOCUS       DAZPBM010000261         4043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPBM010000261 DAZPBM010000000
VERSION     DAZPBM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.264"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            926..1642
                     /gene="nanC"
                     /locus_tag="ACHUZQ_004116"
     CDS             926..1642
                     /gene="nanC"
                     /locus_tag="ACHUZQ_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1027660.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1662..2768
                     /gene="nanM"
                     /locus_tag="ACHUZQ_004117"
     CDS             1662..2768
                     /gene="nanM"
                     /locus_tag="ACHUZQ_004117"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1027661.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2833..3813
                     /locus_tag="ACHUZQ_004118"
     CDS             2833..3813
                     /locus_tag="ACHUZQ_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1027662.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3923..4043
                     /locus_tag="ACHUZQ_004119"
                     /pseudo
     CDS             3923..4043
                     /locus_tag="ACHUZQ_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1219 a          845 c          915 g         1064 t
ORIGIN      
        1 ggagataagg gggaacgcag gagacgcttt ttcatctaca ttcgtgggta aatatggctt
       61 tggtcactgc gacttaaacc ttcctcaatc acagctattc gccgggcgat acggcaaaca
      121 aactaagtga aacatcatcc tgaaaacacc aacatcaaca agcctctcta gatcgacttc
      181 agaagcgacc agttacaagc cacaaatcag gaaacatatt tcatggtgag aattatgatc
      241 agcctgatgg cgggtaaaca cttgttttca ccacgctcac tgagttagct tgcaaagctt
      301 cccttgttta gaccctaaat aaatttttta taaaacaaac agttaaaaga aatcctttag
      361 tttttgaaga ccacctcaag tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa
      421 gatcgacata ttttgtgaca cgaattgcaa atctcgtttt gttgtatggc ttgcgtgatt
      481 tttgatctgg tataacaggt ataaaggtgc accgcgatag tcaatgagac agggcatctc
      541 gcaatctatg gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag
      601 tctgaaagga atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat
      661 ggattgagta agtgatatag cttacgaaca ttcaaatcaa ttaaacatca gaagagattt
      721 tatactcagg tatttaatct gattctctgt ttatttaaat aatgtggaaa gagaattttc
      781 acaggagacc ttatacaaaa atataaaata agctacccgt tgccaaagac actataagcc
      841 tggcaaaaaa tattacacaa cataaatgct aattgtttat gcgggctttg tattgctttc
      901 tgtatcctac aaatgagtga aatttatgaa aaaggctaaa atactttctg gcgtattatt
      961 actgtgcttt tcgtcaccat taatttctca ggctgcgaca ctggacgtac gtggtggata
     1021 tcgtagcgga agccatgcct atgagactcg actcaaagtc agtgagggat ggcaaaatgg
     1081 atggtgggca agcatggaaa gtaatacctg gaataccatt catgataata aaaaggaaaa
     1141 tgccgcactc aatgatgttc aggttgaagt taattacgcg attaaacttg atgatcaatg
     1201 gacggtgcgc ccgggaatgt taacgcattt tagcagcaac ggcacacgct acggacccta
     1261 cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt ggcattcgct atcgttacga
     1321 ctggaaagct taccgacaac aagacttatc cggtgatatg tctcgtgata acgttcatcg
     1381 ttgggatgga tatgtcactt accatattaa tagtgatttc accttcgcat ggcaaacgac
     1441 gctatacagc aaacagaatg attatcgcta tgcaaaccac aagaaatggg cgacggaaaa
     1501 tgcctttgtt ctacaatacc atatgacgcc cgatattacg ccatacatag aatatgacta
     1561 ccttgaccgc cagggtgttt acaacggcag agataattta tcggaaaaca gttatcgcat
     1621 tggtgtgtca tttaaactgt agtagacagg agacagtcac aatgaataaa actataatgg
     1681 cgcttgctat catgatggct tcatttgccg caaacgcgtc tgtattaccg gaaactcctg
     1741 tgccatttaa aagtggtacc ggagcaattg ataacgacac tgtctacatt ggtttaggta
     1801 gcgcaggtac agcatggtac aagctagata cacaggccaa agataaaaaa tggactgcgt
     1861 tagctgcatt ccctggtgga ccgagagatc aagcaacctc tgcatttatt gatggcaatc
     1921 tatatgtgtt tggcggcatt ggcaaaaaca gcgagggatt gactcaggta tttaatgacg
     1981 tacacaaata caaccccaaa accaatagct gggttaaatt gatgtcgctc gcgccgatgg
     2041 gcatggcggg tcatgtgact tttgtacaca acggcaaggc ttatgttact ggcggtgtta
     2101 accagaatat cttcaatggc tattttgaag atctcaacga ggctggaaaa gattcaaccg
     2161 ctatagataa aatcaacgcc cactattttg acaaaaaagc agaagattat ttcttcaata
     2221 agtttctgtt gtcttttgat ccctcaacac agcaatggag ttacgctggc gaatcgccct
     2281 ggtacggaac ggctggtgcg gcggttgtga ataaaggtga taaaacctgg cttattaatg
     2341 gcgaagccaa accaggattg cgaacggatg ccgtatttga acttgatttc accggtaata
     2401 atttaaaatg gaataagctt gatcccgtct catcaccaga tggcgtcgct ggcggttttg
     2461 cggggataag caatgattct cttatatttg ccggaggggc cggattcaaa ggttcacgag
     2521 aaaattacca gaacggtaag aactatgcgc atgaaggcct gaaaaaatca tatagcactg
     2581 atattcatct ttggcataac gggaaatggg ataaatcggg tgaattatcg caaggtcggg
     2641 cctacggagt atcattgccc tggaataata gtttattaat tattggcggt gaaactgcag
     2701 gcggcaaagc ggtgacggat tcagttttaa tttctgtgaa ggataataaa gtcacagtac
     2761 aaaactaacg cttcagggcc ccggtaaagg ggccctgcta tcaatttgtc atttgtatta
     2821 cgcgaaaatt atatgaacgc aataatatcg cccgattatt actatgttct taccgttgct
     2881 ggtcagtcta atgccatggc gtatggcgaa ggactgccat taccggacag ggaagatgcg
     2941 cctcattcca gaattaaaca attagcgaga tttgcgcata cgcatcccgg aggcccgcca
     3001 tgtcacttta acgacattat tccactgact cactgcccac acgatgttca ggatatgcag
     3061 ggttatcacc atcctctggc aacgaatcat caaacacagt acggcaccgt tggccaggca
     3121 ctgcatattg cacggaaatt actgcccttc attcctaata atgcagggat tcttatcgtt
     3181 ccgtgttgcc gtggcggatc ggcttttacc gcgggcagcg aagggacata ttcagaacgg
     3241 cacggagcca gccatgatgc ttgtcgttgg ggaacggata ctccgctgta ccaggattta
     3301 gtcagcagaa cgcgagccgc actggcaaaa aatccgcaga acaaattcct cggcgtatgc
     3361 tggatgcaag gcgaatttga cttaatgacc agtgactacg cgtcacaccc tcaacacttt
     3421 aatcatatgg ttgaagcctt tcgtagggat ctaaaacaat accattctca gcttaataat
     3481 attactgacg cgccatggtt ttgcggcgat accacctggt actggaaaga aaatttcccg
     3541 cattcgtatg aagctattta tggcaattat caaaataatg ttttagccaa tattattttc
     3601 gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg cgcctgatga agacccggac
     3661 gatttaagca cgggatatta cggttcagcg taccggtcac cggagaactg gacgacggca
     3721 ctgcgaagca gtcatttcag cacggcagcc cgtcggggga ttatttctga cagatttgta
     3781 gaagcaattt tgcagttttg gcgcgaaagg tgagcgggga gtttattcat gggggatgtc
     3841 cttgttcgtt ctcagagtag acaacaaaaa acaaataaga tcaaggtaat atctcttcac
     3901 ttgcgggctc gaacagcagg caattgccaa tataataatc tggcacaagg gtgaaaaacg
     3961 cgcaccgcat aaaccgttgt tattgcttac agattagccg gatgccttaa tagacatccg
     4021 cgccttttcg attatggctc gga
//
LOCUS       DAZPBM010000262         4041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPBM010000262 DAZPBM010000000
VERSION     DAZPBM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.265"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            376..483
                     /locus_tag="ACHUZQ_004120"
     CDS             376..483
                     /locus_tag="ACHUZQ_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1027663.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHUZQ_004121"
     CDS             complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHUZQ_004121"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1027664.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHUZQ_004122"
     CDS             complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHUZQ_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1027665.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHUZQ_004123"
     CDS             complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHUZQ_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1027666.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1032 a         1011 c         1052 g          946 t
ORIGIN      
        1 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
      181 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
      361 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
      421 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
      481 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      541 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
      601 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
      661 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
      721 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
      781 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
      841 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
      901 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
      961 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
     1021 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
     1081 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
     1141 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
     1201 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
     1261 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
     1321 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
     1381 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
     1441 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
     1501 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
     1561 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
     1621 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
     1681 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
     1741 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
     1801 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
     1861 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
     1921 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
     1981 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
     2041 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
     2101 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
     2161 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
     2221 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
     2281 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
     2341 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
     2401 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
     2461 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
     2521 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
     2581 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
     2641 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
     2701 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
     2761 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
     2821 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
     2881 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
     2941 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
     3001 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
     3061 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
     3121 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
     3181 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
     3241 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
     3301 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
     3361 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
     3421 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
     3481 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
     3541 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
     3601 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
     3661 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
     3721 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
     3781 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
     3841 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
     3901 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
     3961 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
     4021 gattatcagt ttactagcga a
//
LOCUS       DAZPBM010000263         4037 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPBM010000263 DAZPBM010000000
VERSION     DAZPBM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.266"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(200..847)
                     /gene="ychE"
                     /locus_tag="ACHUZQ_004124"
     CDS             complement(200..847)
                     /gene="ychE"
                     /locus_tag="ACHUZQ_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1027667.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1325..4000
                     /gene="adhE"
                     /locus_tag="ACHUZQ_004125"
                     /gene_synonym="adhC"
     CDS             1325..4000
                     /gene="adhE"
                     /locus_tag="ACHUZQ_004125"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1027668.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1118 a          996 c          941 g          982 t
ORIGIN      
        1 cttcagcaag cccaggatta tgatgtggcg aaaataacat gatggtgagg agaagcagtt
       61 aaggaaatcc ttataactaa ctgtaatttg agaagaatat ttttacctat tccttttgct
      121 tttgataact gcgcgggaac aaagtaaaaa taatgacgaa gtaaacggag catggcagct
      181 ccgtttcatt tgaaaggaat taattaagca ggccggggaa aatcccctta ataccagtaa
      241 cgataaattc aatccccaat gccatcagca atagccccat aatacgcgta atcacgttga
      301 tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat gcggaacaat ccccagcaac
      361 ataaagcgaa caatgcaata gccacaaaga aaccaaacag atagctaatg ctgtgataac
      421 gcgtacccca gacgatggta gaactgatcg cccctggccc cgccatcaac ggcaacgcca
      481 atggcaccac accaatgctt tcacgtaccg cggtttctga tttttcttgc ttgttctgtt
      541 tatcctcgcc aagcttgccg ctgatcatcg acatcgctat cgtcaccacc aggatgcccc
      601 ctgcgatacg gaacgaatca attgatatac caaaaagttg cagaatcgtg tcgccgagaa
      661 aaagcgagat ccacaagata atggccacag acaggttggc tgtaaggtta gttttgtttc
      721 gcgctgccgc tgtctgataa ctggtcatgc tgataaagac gggaataatc cctaccgggt
      781 tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac gggaaaatcc aaaaaggtct
      841 gaatcacggt tagctccgaa gcaaaagccg gataatgtta gccataaatt aaggttgaaa
      901 agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac ttaaaattgc
      961 tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac caaatcatac
     1021 tacaatttat taactgttag ctataatggc gaaaagcgat gctgaaaggt gtcagcttag
     1081 caaaaatttg atttggatca cgtaatcagt acccagaagt gagtaatctt gcttacgcca
     1141 cctagaagtg acgcattaga gataataact ctaatgttta aactctttta gtaaatcaca
     1201 gtgagtgtga gcgcgagtaa gcttttgatt ttcataggtt aagcaaatca tcaccgcact
     1261 gactatactc tcgtattcga gcagatgatt tactaaaaaa gtttaacatt atcaggagag
     1321 cattatggct gttactaatg tcgctgaact taacgcactc gtagagcgtg taaaaaaagc
     1381 ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac aaaatcttcc gcgccgccgc
     1441 tctggctgct gcagatgctc gaatcccact cgcgaaaatg gccgttgccg aatccggcat
     1501 gggtatcgtc gaagataaag tgatcaaaaa ccactttgct tctgaatata tctacaacgc
     1561 ctataaagat gaaaaaacct gtggtgttct gtctgaagac gacacttttg gtaccatcac
     1621 tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg accactaacc cgacttcaac
     1681 tgctatcttc aaatcgctga tcagtctgaa gacccgtaac gccattatct tctccccgca
     1741 cccgcgtgca aaagatgcca ccaacaaagc ggctgatatc gttctgcagg ctgctatcgc
     1801 tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg aactgtctaa
     1861 cgcactgatg caccacccag acatcaacct gatcctcgcg actggtggtc cgggcatggt
     1921 taaagccgca tacagctccg gtaaaccagc tatcggtgta ggcgcgggca acactccagt
     1981 tgttatcgat gaaactgctg atatcaaacg tgcagttgca tctgtactga tgtccaaaac
     2041 cttcgacaac ggcgtaatct gtgcttctga acagtctgtt gttgttgttg actctgttta
     2101 tgacgctgta cgtgaacgtt ttgcaaccca cggcggctat ctgttgcagg gtaaagagct
     2161 gaaagctgtt caggatgtta tcctgaaaaa cggtgcgctg aacgcggcta tcgttggtca
     2221 gccagcctat aaaattgctg aactggcagg cttctctgta ccagaaaaca ccaagattct
     2281 gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa aactgtcccc
     2341 gactctggca atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag cagagaaact
     2401 ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac actgaccagg ataaccaacc
     2461 ggctcgcgtt tcttacttcg gtcagaaaat gaaaacggct cgtatcctga ttaacacccc
     2521 agcgtctcag ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt ccctgactct
     2581 gggttgtggt tcctgggggg gtaactccat ctctgaaaac gttggtccga aacacctgat
     2641 caacaagaaa accgttgcta agcgagctga aaacatgttg tggcacaaac ttccgaaatc
     2701 tatctacttc cgccgtggct ccctgccaat cgcgctggat gaagtgatta ctgatggcca
     2761 caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac aatggttatg ctgatcagat
     2821 cacttccgta ctgaaagcag caggcgttga aactgaagtc ttcttcgaag tagaagcgga
     2881 cccgaccctg agcatcgttc gtaaaggtgc agaactggca aactccttca aaccagacgt
     2941 gattatcgcg ctgggtggtg gttccccgat ggacgccgcg aagattatgt gggttatgta
     3001 cgaacatccg gaaactcact tcgaagagct ggcgctgcgc tttatggata tccgtaaacg
     3061 tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg atcgctgtca ccaccacttc
     3121 tggtacaggt tctgaagtca ctccgtttgc ggttgtaact gacgacgcta ctggtcagaa
     3181 atatccgctg gcagactatg cgctgactcc ggatatggcg attgtcgacg ccaacctggt
     3241 tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg gacgcagtaa ctcacgccat
     3301 ggaagcttat gtttctgtac tggcatctga gttctctgat ggtcaggctc tgcaggcact
     3361 gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa atccggtagc
     3421 gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc gcatttgcga acgccttcct
     3481 gggtgtatgt cactcaatgg cgcacaaact gggttcccag ttccatattc cgcacggtct
     3541 ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat gcgaacgaca acccgaccaa
     3601 gcagactgca ttcagccagt atgaccgtcc gcaggctcgc cgtcgttatg ctgaaattgc
     3661 cgaccacttg ggtctgagcg caccgggcga ccgtactgct gctaagatcg agaaactgct
     3721 ggcatggctg gaaacgctga aagctgaact gggtattccg aaatctatcc gtgaagctgg
     3781 cgttcaggaa gcagacttcc tggcgaacgt ggataaactg tctgaagatg cattcgatga
     3841 ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac agatcctgct
     3901 ggatacctac tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa aagaagccgc
     3961 tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa tcagtagcgc tgtctggcaa
     4021 tataaacggc cccttct
//
LOCUS       DAZPBM010000264         3995 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPBM010000264 DAZPBM010000000
VERSION     DAZPBM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.267"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(17..802)
                     /locus_tag="ACHUZQ_004126"
     CDS             complement(17..802)
                     /locus_tag="ACHUZQ_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027669.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(1132..1208)
                     /locus_tag="ACHUZQ_004127"
     tRNA            complement(1132..1208)
                     /locus_tag="ACHUZQ_004127"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1172..1174),aa:Asp,seq:gtc)
     gene            complement(1341..2072)
                     /gene="dnaQ"
                     /locus_tag="ACHUZQ_004128"
     CDS             complement(1341..2072)
                     /gene="dnaQ"
                     /locus_tag="ACHUZQ_004128"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1027670.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            2137..2604
                     /gene="rnhA"
                     /locus_tag="ACHUZQ_004129"
     CDS             2137..2604
                     /gene="rnhA"
                     /locus_tag="ACHUZQ_004129"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1027671.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2601..3323)
                     /locus_tag="ACHUZQ_004130"
     CDS             complement(2601..3323)
                     /locus_tag="ACHUZQ_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1027672.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            3357..>3995
                     /gene="gloB"
                     /locus_tag="ACHUZQ_004131"
     CDS             3357..>3995
                     /gene="gloB"
                     /locus_tag="ACHUZQ_004131"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1027673.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQI"
BASE COUNT         1106 a          935 c          873 g         1081 t
ORIGIN      
        1 cggcagatat aatttattat tgatagcggt taaccaaatc ttttaccatc gctttatagg
       61 tcttttgctt aagttgcatg atttgctgct ctgtcatatc agtcgtactg gcttcattct
      121 tgtcgggcaa tggtggaagc actttatatt cataattcac cggcgaccag gcagcccact
      181 cacctgtagc tacatccacc agcgtgaatc gtaccagata gcgcagtgac atcgtgtcag
      241 tcaatttttg atttttataa tctgaccaaa ctattgattt caatccagag tcatattttc
      301 ctgtctgcaa cgtatcctgg aaaacaataa tcgctttctg atgccctttt gcagccacaa
      361 aacgcagtgc ctgcatccag ttcatattct cagcactggc aacatcctcg ttctcatttt
      421 tatctttgtt tttgttacaa gtcagaggct tttgcctgtc cgggatacca gagaatgtgg
      481 aaacagtata atattttcgc atctcctcct gcataatggt ttccggtgcg cggttgccgg
      541 attgaacaag tattacagga gaattaaggg gaacgcggaa ctcatctcca tcaagcgcag
      601 cttgaatatc ctcttcggat acggccagag tggtttcatt accgaataaa tctttgtctg
      661 tcagttgctg gggagtgttt ttattaatat tactttgtgt gacttgagct tttttcttct
      721 tttcatcaat agaagattca gaggcacatc ctgcaagcag cgagaataat acacatatac
      781 aacaaaccct tcttaaattc ataatcggac actccctcgc ctgtcataat catggtaatg
      841 tttagctaac gatttttaat tatatactcc acttgaatca gaacaaacaa aaataacagt
      901 atatataaat tcattaaatc aatagaaaaa tgaaaatcat aacattcaaa tatatttaaa
      961 ttgaatgatt cgatagtatg attaaatatt aatgatatgg tctaccatat gtgattaaca
     1021 catttacagc ataacaaaac catagaccga agaaatagcg ggatttaaat gcaaaaaaac
     1081 cgcaaaatta aaaatcttgc ggttctctga actcattttc atgagtgaat ttggcggaac
     1141 ggacgggact cgaacccgcg cccccctgcg tgacaggcag gtattctaac cgactgaact
     1201 accgctccgc gttgtgttcc gttgggaacg aggcgaatat tacggattgc ctcacccttc
     1261 gtcaacggtt tttctcatct tttgaatcgt ttgctgcaaa aatcgcccaa gccgctattt
     1321 ttagcgcctt ttacaggtat ttatgcccgc cagaggcagc ttccgccctt cttctccacc
     1381 agatcaagac gggcttcatg agctgcaagc tcttcatctg tcgcaaaaac aacgcgtaac
     1441 ttacttgcct gacgtacaat gcgctgaatt gttgcttcac cttgttgctg ctgtgtctct
     1501 ccttccatcg caaaagccat cgacgtttga ccaccggtca tcgccagata aacttccgca
     1561 aggatctgtg catcgagtaa tgccccgtgt agcgttcgtt tactgttatc tatttcgtag
     1621 cgggcacata acgcatcgag gctgttgcgc ttaccaggaa acattttcct cgccaccgca
     1681 aggctatcgg tgaccttaca gaaggtattg gtcttcggaa tatcgcgctt aagcaacgaa
     1741 aactcgtagt ccataaagcc gatatcgaac gctgcgttat ggatcaccaa ctccgcgccg
     1801 cggatatagt ccatgaactc atcggctact tcggcaaacg tgggcttatc gagcaaaaat
     1861 tcatcggcaa taccatgtac gccaaaggct tccggatcca ccagccgatc gggtttgaga
     1921 taaacatgga agttattgcc cgtcaggcga cggttcacca cttcaacggc accaatctca
     1981 atgatcttgt ggccttcata gtgcgcacca atctggttca taccggtggt ttcggtatcg
     2041 agaacgatct ggcgtgtaat tgcagtgctc atagcggtca tttatgtcag acttatcgtt
     2101 ttacagttcg attcaattac aggaagtcta ccagagatgc ttaaacaggt agaaattttc
     2161 accgatggtt cgtgtctggg caatccagga cctgggggtt acggcgctat tttacgctat
     2221 cgcggacgcg agaaaacctt tagcgcaggc tacacccgca ccaccaacaa ccgtatggag
     2281 ttgatggccg ctattgtcgc gctggaggcg ttaaaagaac attgcgaagt cattttgagt
     2341 accgacagcc agtatgtccg ccagggtatc acccagtgga tccataactg gaaaaaacgt
     2401 ggctggaaaa ccgcagacaa aaaaccagta aaaaatgtcg atctctggca acgtcttgat
     2461 gctgccctag ggcaacatca aatcaaatgg gaatgggtta aaggccatgc cggacacccg
     2521 gaaaacgaac gctgtgatga actggctcgt gctgcggcaa tgaatcccac actggaagat
     2581 acgggctacc aagtcgaagt ttaagcttgt ggtttacgac attgccgggt ggctccaacc
     2641 gcctggcgaa ttcgtggctt gtttttactc tgtttcatcg ggtttagcgt taaaggaata
     2701 gtccgtttcc gggcaacaat aagttgtaag caaccaagcg caggaatatg cgcattcaat
     2761 agttttcctc cgtgtttgtt ccacgggaga acgtggaaac ggctggcgtg cagcacttca
     2821 aaattcaaca aagagagcca gtccagttgc cgcatcagag taaacatccg gctgttatag
     2881 ggcgaggttt ttcgcaatac cggcacaagt ttgcgtaatc ccattaaact aatgggattg
     2941 aagccactaa tcaccagcca gccatcatca atcaataccc gatcggtttc acgcaataaa
     3001 cgatgcggat cggtgcacca cggcaatgta tgtgccagta gacaaacatc aacggattta
     3061 tcggcaaaag gaagatgaag tggatccgcc tgcacctgga cgggcattcc ttgcgcagaa
     3121 acattcactt gatgagaaac cgcgcacgct tcgcaattga tttctgcgct taaattgcca
     3181 atcttaagca gatgaaaacc atacatttta gtgaaccacg ggttgagctg gcgctccagc
     3241 gccttgcgat aaagctctcc ccagggcaaa tcgccccagc gatcaggagc cacgacagtt
     3301 tgagggactc ttgccggttt catcacaacc ttccgtttca cactaagagg taatctatga
     3361 atcttaacag tattcccgcc tttgatgaca attacatctg ggttttgaat gatgaagcag
     3421 gtcgctgcct gattgtcgat cccggagacg cagagccagt attaaacgcc attgccgcca
     3481 ataactggca accggaggcc atatttctca cccaccatca tcacgatcac gttggcggcg
     3541 taaaagaact ggtggaaaag tttccgcaaa ttgtggtgta tggtccacaa gagacacaag
     3601 ataagggaac aacacaggta gtcaaagatg gcgaaactgc cttcgttttg gggcatgaat
     3661 ttagtgtaat tgccacaccg ggtcacactt taggacatat ctgttacttc agtaaacctt
     3721 atctattttg cggcgacacg ctgttttctg gtgggtgtgg taggttgttt gaagggacag
     3781 catcacaaat gtatcaatca cttaataagt taagtgcgtt acctgacgat acattggtat
     3841 gttgtgctca tgaatatacg ttatcaaata tgaagtttgc tttgagtatt cttccgcacg
     3901 atttgtccat aaatgattat tatcgtaaag ttaaggagtt acgggcaaaa aatcaaataa
     3961 cactacccgt aattctgaaa aatgagcggc aaatt
//
LOCUS       DAZPBM010000265         3962 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPBM010000265 DAZPBM010000000
VERSION     DAZPBM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.268"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUZQ_004132"
     CDS             complement(<1..242)
                     /locus_tag="ACHUZQ_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1027674.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHUZQ_004133"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHUZQ_004133"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1027675.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHUZQ_004134"
     CDS             2255..3904
                     /locus_tag="ACHUZQ_004134"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1027676.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1032 a         1001 c          929 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 gg
//
LOCUS       DAZPBM010000266         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPBM010000266 DAZPBM010000000
VERSION     DAZPBM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.269"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            30..968
                     /locus_tag="ACHUZQ_004135"
     CDS             30..968
                     /locus_tag="ACHUZQ_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1027677.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="ACHUZQ_004136"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="ACHUZQ_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="ACHUZQ_004137"
     CDS             2310..2603
                     /locus_tag="ACHUZQ_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1027678.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2768..3946
                     /gene="fadK"
                     /locus_tag="ACHUZQ_004138"
                     /pseudo
     CDS             2768..3946
                     /gene="fadK"
                     /locus_tag="ACHUZQ_004138"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1003 c         1088 g          926 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgcgg tggtgaatct cgatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaat
//
LOCUS       DAZPBM010000267         3939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPBM010000267 DAZPBM010000000
VERSION     DAZPBM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.270"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..588
                     /gene="fimZ"
                     /locus_tag="ACHUZQ_004139"
     CDS             <1..588
                     /gene="fimZ"
                     /locus_tag="ACHUZQ_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR1027679.1"
                     /translation="RMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDL
                     PGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPV
                     QMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLL
                     LSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(591..1106)
                     /gene="sfmF"
                     /locus_tag="ACHUZQ_004140"
     CDS             complement(591..1106)
                     /gene="sfmF"
                     /locus_tag="ACHUZQ_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR1027680.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1117..2124)
                     /gene="fimH"
                     /locus_tag="ACHUZQ_004141"
     CDS             complement(1117..2124)
                     /gene="fimH"
                     /locus_tag="ACHUZQ_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1027681.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2137..3911)
                     /gene="fimD"
                     /locus_tag="ACHUZQ_004142"
                     /pseudo
     CDS             complement(2137..3911)
                     /gene="fimD"
                     /locus_tag="ACHUZQ_004142"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1097 a          971 c          861 g         1010 t
ORIGIN      
        1 agaatgtcta ttgaagttct gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg
       61 gatgattacc gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg
      121 gatatagact tgcccggaac agacggtttt accttcctga aaaggatcaa acaaatccag
      181 agcacagtga aagtgttatt tttatcatcg aaatcagaat gcttttatgc tggcagagcg
      241 atacaagctg gtgctaacgg ttttgtcagt aaatgcaatg atcagaatga tatttttcat
      301 cccgttcaga tgatcctctc cggatacacg ttttttccta gcgaaacgct caactatata
      361 aaaagcaata aatgtagtac gaatagttca acggtcactg tgctatctaa tcgtgaagtg
      421 accatattac gttatctggt tagcggatta tctaataaag aaattgccga taagttatta
      481 cttagcaata aaacagttag tgcgcataaa tctaatattt atggcaaact aggtttgcat
      541 tcaattgtag agcttatcga ctacgccaaa ttatacgaat taatataata ttaattataa
      601 ttgatcataa atatcgcatc cgctttctcc acacctggct gaacaccgct ggctaacgct
      661 cgataattgg caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcggg
      721 ctcgcctccc ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgacggtt
      781 tgtgccattg cgggatcatc cagagccagc aggttggtat cggatgccgg cgttcccgta
      841 aataaaatcg caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt
      901 gccgtggtat cgccagcgtt cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc
      961 ttatcaatat ccgcagtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg
     1021 atattaatcg ttcccaaagg gtcagctgcc catccactgg aactccacag taacccgcag
     1081 aaacagctaa agagtactct tctcattctc cgtacctcat tgataatcga cgcgtaaata
     1141 ccccagcgcg ctaaacggcc cttcggtcgg tttttgaccg gtaatactga taggccaggc
     1201 gcgaagtgtg acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag
     1261 atcgttaggc gtgatcggcg tatcgttctg atcggcgaca ataaaaccta aatcctgatt
     1321 gtccgacacc atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc
     1381 ctgcgcagca acattcgtac atttgatcgc aatgctcttg gtttgcggca tgacgccagc
     1441 tggtcgattc cccggccctg ccgcactaaa taacgatgcg ccgatatcac caaaatcaaa
     1501 ttcaacaatc tgcccggcat ttaattcgca gttttgcggc acttcaaccc ggccaccaaa
     1561 actaatggta taaacaggtg taaccaacgg atcgtaaggc gttgatgtca catacacggt
     1621 aaacatcacc tgtctgggga tctccaccat gttaataaag ggacgaataa cctttaaacg
     1681 aaatatcaaa cgagaatcca tcaccccgaa cggaaggttt tgcgaaacgt taggatctgt
     1741 tcccatccga atataatttt gcggggggaa ctgaatatcc atcacactat caaccagact
     1801 catcgcacct aatagataat catttaattg catatattta taattatcga tagtttcctg
     1861 aacaataaag ttagtgacat aactacggta tgtataattc accagcgtgc cgggtggaca
     1921 aattgctgag acacctaccc agccggattt ttccggtaaa acaacaatct gccctggctg
     1981 gttattggtg ctattaaaga catttgagag atcatagtgt tcatcttctg ctacgccagt
     2041 actatttgca caaacagtag cccaggatat gtttgccaga cacaaacatg ccattgccaa
     2101 taataatctg cagattatct tcattgataa tttcctttat gagccaggat gtgtgcaaac
     2161 tgccgaaata acagtgacgg cttgctgtaa gctttgcttt ggcaatacat agtgcgcaat
     2221 gcagcgtgag ttcgcgccat ctccccactg aaccagtaat tcaccagaca atggcgcacc
     2281 acttaaataa acttgcccgt catcacccac catactggtt attccggtac tgttttcccg
     2341 taccagtgat ccaaacggca ccggttttcc gccctgggta acggtaatga gcgcccgcac
     2401 gcctatgcgg gtatcaaaat tggcacgaac caacgcgcct tgcgtcggca caacgctgct
     2461 aatatttttt tcaacatcga tggaattccc catcgtattg gtatcaagcg cgatacggtt
     2521 ataccgataa accgtggcat acggcatcac cgcatagccg cgccagtcgg ttaaaatgcc
     2581 agtttgattt tcaatgcgta caccgcctgc gccaggcgct ttaatcaaaa cattggtatc
     2641 ccctaaaggc tggctcagtg ttatgccatt ttcatgcccg accacaccgc cagacagctg
     2701 ccagttaacg tcatgttgat cgcggtcgta gttatacccg acccccagcg taccgtaagt
     2761 ggcctgccag tttgccgtgg cgctgcccgt gtacccatta ttcgaggtat cgccctggct
     2821 tacgtgataa ctcaggttgt ggccttccag taaggtccca ccaacgcctg ccagccagct
     2881 attttgcccg ttgctgttac ggttagcgtt caaggaggca taagcgcggt cgagcgcatt
     2941 ttcccgggtg tagcgacgtt tggtcaaaac attgaaaggc actgaaacat taagtccgac
     3001 aatatgttcg ttatcgggga tccctacaga ttcattccac gaaaatgaga gtgaataact
     3061 gatgccaacc cagctgctgg tataccccac ctgataccag gtatctgaat ccgaagtatt
     3121 ccagtatttt tgatgggtac cagagatata aagcgagcca aagtcattaa gtgattgtga
     3181 aatatttaac tgcaaacggt ctttgcggct aaagcgtaaa ttgtggtaat tcacgattat
     3241 cggttcatcg cgatgctcac cgtcgtaatc gtaatcatat tcgtacccct ccattcgacg
     3301 atacgcaaca tcatcaaggg tataaaaacc ttgtgtcgaa tagcggtaac ccattaactg
     3361 aaaattggtg ccgaaggtgt acatcgattt cgcatagagg aagcgaatgg aatccccctc
     3421 atgacgactg tcgtcggcta actggctgcg cgcatgggtc acatccagcg acactgcgcc
     3481 ccagttcccg aggttgcgcc ccagccctaa taaaaaggcg gtgtaattgg cagataattg
     3541 cgtcccgccg taggcagtaa attcctgtgg taaaccaccg agtgccgtgc cctgaaaaaa
     3601 gaaaggtgat gattgctgac tattaccgct acgaaaatcg cccgccgtca ggtcaaattt
     3661 gaaacgccct tcgcgttgta aaatcggcac tgttgaatac ggaattgtgt aattctgcca
     3721 tcgcgctcgt cgatggtgac atccagatcg ccatttgaag atgtcgggtg caaatcagta
     3781 atttcaaaag caccggggga aacatagctt tgatagataa taaaaccatt ttgccgaatc
     3841 gttagctggg ccgccgtacg ggcaatccca cgtaccgttg gggcaaaccc ttgctggcta
     3901 tctggataca tattatcgga agaataaagc cgtacacca
//
LOCUS       DAZPBM010000268         3910 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPBM010000268 DAZPBM010000000
VERSION     DAZPBM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.271"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            26..592
                     /locus_tag="ACHUZQ_004143"
     CDS             26..592
                     /locus_tag="ACHUZQ_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1027682.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            596..1171
                     /locus_tag="ACHUZQ_004144"
     CDS             596..1171
                     /locus_tag="ACHUZQ_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1027683.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1213..2265)
                     /locus_tag="ACHUZQ_004145"
     CDS             complement(1213..2265)
                     /locus_tag="ACHUZQ_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1027684.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2490..3410
                     /gene="lpxL"
                     /locus_tag="ACHUZQ_004146"
     CDS             2490..3410
                     /gene="lpxL"
                     /locus_tag="ACHUZQ_004146"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1027685.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3582..3767
                     /locus_tag="ACHUZQ_004147"
                     /pseudo
     CDS             3582..3767
                     /locus_tag="ACHUZQ_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          988 c          952 g          999 t
ORIGIN      
        1 tcgcaaattc gtattggatg cacttatgtc attcacaaat acccctgaac gctatggtgt
       61 tatatctgcg gccttccatt ggctaagcgc aattattgtc tacggcatgt ttgctttagg
      121 tctgtggatg gtcacgctca gttattacga tggctggtat cacaaagcac ccgaactgca
      181 taaaagcatc ggtattttgt taatgatggg gctggttatt cgcgttctgt ggcgtgtcat
      241 atctccaccg ccgggtccgc tgccgagtta ttcgcccatg actcgtcttg ccgcaaaggc
      301 tggacatctc gcattgtacc tattgctgtt cgccatcggc atcagcggct atctgatctc
      361 aactgccgat ggtaaaccga tcagcgtttt tggctggttt gacgtccccg cgacccttgc
      421 cgacgctggc gcacaggcag actttgccgg tgccctgcat ttttggctcg cgtggagcgt
      481 cgtagtactg tccgttatgc acggatttat ggccctgaag catcatttca tcgataaaga
      541 cgacactctg aagcgcatgc tgggaaagtc gtcatctgac tatggagtat aaaaaatgaa
      601 aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc tctgccggtt cagcggttgc
      661 cgccgattac aaaattgaca aagaaggtca gcacgccttt gttaatttcc gcatccagca
      721 ccttggctat agctggttat acggcacctt taaagatttc gacggtactt ttacctttga
      781 cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt aacaccacca gcgtcgatac
      841 taatcacgcc gaacgcgata aacatcttcg cagtgcagat ttcctcaata ccgcaaaata
      901 tccacaggca acattcacct ccaccagcgt gaagaaagac ggtgacgaac tggatattac
      961 cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg ctggaagcga aattaattgg
     1021 tcagggcgac gacccatggg gtggtaaacg tgcaggcttc gaggccgaag gcaaaattaa
     1081 gctcaaggac ttcaatatca agacagattt aggtccagct tctcaggaag tggatctgat
     1141 tatttcagtg gaaggcgtac agcagaagta agcgtgaaat gttgccggat ggcacgcatc
     1201 cggcaatgat atttattctg ttggatcagg aatgcccagt gttgtattca gacgtccacg
     1261 agacttatta aagatcttat tgccattttc acgtcctgcc cgacggcgtc gctgttcttc
     1321 tggcggtaac gcgctttctt cgcagcaaat ctcactacaa caacctttgt atttttccgc
     1381 gcatactgga cactgaataa acagcaggtg gcagccatca tttttacagt tggtatggct
     1441 gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc tcatccgata tacgttcgcc
     1501 catccgctcg tcaaaaacaa aatttttgcc aataaaacgc accggcaagc cctgctcgcg
     1561 cgccttacgg gcgtattcaa taattccgcc ctcgatatgc cagactttgt tgaatccgtt
     1621 atgtttcatc caggcactgg ccttttcaca acgaatgccg ccggtgcagt acatgacgat
     1681 ttttttatct ttatgtgcct gcatcatctc gactgctttt ggcagctgct cacggaaggt
     1741 atctgccgga atttcgagcg cgttttcaaa gtgccccact tcatactcat agtggttacg
     1801 catgtcgata aatagtgcat cgggatcgtc aagcatggcg ttcacttccg ccgcttgcag
     1861 atactcacca acattgctgg catcaaagtg aggatcgtca ataccgtcgg caacaatgcg
     1921 atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg tcatcatcca acgcgatatt
     1981 caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc tgcgcgcgaa atgtttcaac
     2041 attgctcgca ggtacgctga tttgcgcgtt aatgccctca tgcgccagat acactcgccc
     2101 aaaaacattc agcgcggtaa acagctgata taaagcgtca cgggtcgcct taggatcggc
     2161 gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt tcgctctcaa ccaacatttt
     2221 ggcttttaac gcgtcgttgg aaatgcggtt gtgtaacact ggcatggtgt acggttcctg
     2281 cgagatggga aagtaaaaat ccgcggcatg atatagcaat tatcgataat taacatccac
     2341 acattttacg ctacatttgc gcattaaaaa ttatttgtta tttacaagcg cggcaatttc
     2401 gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa atgcgacaat acatacaatt
     2461 gcccgaatag gttgaaaaac aggattgata tgacgaatct acccaagttc tccaccgcac
     2521 tgcttcatcc gcgttattgg ttaacctggt tgggtattgg cgtactttgg ttagtcgtgc
     2581 aattgcccta cccggttatc taccgcctcg gttgtggatt aggaaaactg gcgttacgtt
     2641 ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga actgtgcttc ccggaaatga
     2701 gcgaacaaga acgccgtaaa atggtggtga agaatttcga atccgttggc atgggactga
     2761 tggaaaccgg tatggcgtgg ttctggccgg accgccgaat cgcccgctgg acggaagtga
     2821 tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg cggcatcctg ttagttggca
     2881 tccattttct gacactggaa ctgggcgcgc ggcagtttgg tatgcaggaa ccggggattg
     2941 gcgtttaccg cccgaacgat aatccactga ttgactggct acaaacctgg ggccgtttgc
     3001 gctcaaataa atcgatgctc gaccgcaaag atttaaaagg catgattaaa gccctgaaaa
     3061 aaggcgaagt ggtctggtac gcaccggatc atgattacgg cccgcgctca agcgttttcg
     3121 tcccgttatt tgccgttgag caggctgcga ccacgaccgg aacctggatg ctggcacgga
     3181 tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa gccagatggc aaagggtatc
     3241 aattgattat gctgccgcca gagtgttctc cgccactgga tgatgccgaa actaccgccg
     3301 cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc accagagcag tatatgtggt
     3361 tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc acgctattaa atctcccctg
     3421 ccggatgctt cagaatggca tccggcatta ccacagcaaa tccccctgat ttagcgataa
     3481 aagctctctg gattgcgccc cctggaagtc gggcgcataa ttagtgtgct tatcttttct
     3541 tcttatgttc accgcgcctg gcgcaccaac agcggattgc tatgtcaccc tgtggaaatg
     3601 acacccctat aaactggaaa cgaaacctga tcgtcgcctg gctaggctgt tttcttaccg
     3661 gtgccgcctt cagtctggta atgcccttct tacccctcta cgttgagcag cttggcgtta
     3721 ccggtcactc cgccctgaat atgtggtccg gtattgtctt cagtataata ccttcctaaa
     3781 tacactacct gagagaagag atgaaaagag ctatcagcta catgaggttt tcaactgaga
     3841 agcagataaa aggggactct atacgaagac agtctaagct ggtcactgac tggttatcaa
     3901 agaaccctga
//
LOCUS       DAZPBM010000269         3881 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPBM010000269 DAZPBM010000000
VERSION     DAZPBM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.272"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(95..307)
                     /gene="cspA"
                     /locus_tag="ACHUZQ_004148"
     CDS             complement(95..307)
                     /gene="cspA"
                     /locus_tag="ACHUZQ_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1027686.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(588..878)
                     /locus_tag="ACHUZQ_004149"
     CDS             complement(588..878)
                     /locus_tag="ACHUZQ_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR1027687.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1312..2022
                     /locus_tag="ACHUZQ_004150"
     CDS             1312..2022
                     /locus_tag="ACHUZQ_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1027688.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2072..3046)
                     /gene="ghrB"
                     /locus_tag="ACHUZQ_004151"
     CDS             complement(2072..3046)
                     /gene="ghrB"
                     /locus_tag="ACHUZQ_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1027689.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3150..3809)
                     /locus_tag="ACHUZQ_004152"
     CDS             complement(3150..3809)
                     /locus_tag="ACHUZQ_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR1027690.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT          942 a          952 c          962 g         1025 t
ORIGIN      
        1 ttattacgcg atcgattatt tatttcctga aaaacaaata aaaaaatccc cgccaaatgg
       61 cagggatctt agattctgtg cttttaagca gagattacag gctggttacg ttaccagctg
      121 ccgggccttt agcgccgctt tcgatggtga aggacacttt ctgaccttcg tccagagatt
      181 tgtaaccatc gttctggata gcagagaagt gtacgaacac atctttagag ccatcgtcag
      241 gagtgatgaa gccgaagcct ttgtcagcgt tgaaccattt tacgatacca gtcattttac
      301 cggacatagt gtattacctt taataattaa gtgtgccttt cggcgatatg gcgtgcttta
      361 cagattttga agcgttaaag gaatgtgcac tacgaggggt atcaacgata actcttgaag
      421 ggacttgcct tactacactg ctttaatggt ctgtacgtca aaccgttgat gtgcattaag
      481 ccacgcattg gcgggtgatg caacaattat ttttcatatt tatgattaat cggttggaaa
      541 acggtcctgt catcaggacc gtaaacagca ataaagtgga taaaagtcta ttccatcaac
      601 tgcttactta atgccgggtt ggcttgaatc aaacgcatca attttagttc ggcacttgaa
      661 ggcttcacgc gtctggattc ccattccttt accatggcga ctgatacgcc caaaacccgg
      721 gcgaaatcat cgatttttaa ccctgtccct tttcgtaact gctcaatttc ggtacaggac
      781 gttgttctgt gcgtcagggt aattttctgc gtttcatctt taaaaacaat ctgttccagg
      841 ctgctcaaca gctcatgcat tggatcttta tattccattg aaaactcctc aaatcacact
      901 gcgggatcgt gaattgcatc gaagctcatt aagaatagtc gggaaaagca gacacagcgg
      961 ggtgaagtca gggcatgctc tggctatgat tcgattcagc ctgtcattta gcgtgactta
     1021 tagccaccta acgatctgat aaatgccgat atatctctta ccactcctgt tttgtaaaga
     1081 tttcgaaaat ctaaccttct gcgaaggcgt aaatctctgg ctacaaaaaa ggcaacggca
     1141 ctatcatttt ttaacaatga aacaacaaat taaccttgtt tcattttctc catgatttta
     1201 ccaggaagat tacgtagaaa gggtatcctg caagcctgtc ctggactatc cttgtcacgt
     1261 cagacacgcg tgtgtcgttg tgcgcttttt ttgggtgaaa ggagtaagaa aatggcgaca
     1321 ggaaagtcct gctctcgctg gtttgcgcct cttgcggcgt tattaatggt agttagcctg
     1381 agtgggtgtt ttgataaaga aggcgatcag cgtaaagcgt ttatcgactt cctgcagaat
     1441 acagtgatgc gtagcggtga acgtctacca accctgactg ccgatcagaa aaaacagttt
     1501 ggtccttttg tctctgatta cgcgattctg tatggttatt ctcagcaggt gaatcaggcg
     1561 atggattctg gtctgcgccc ggtcgtagac agcgttaacg caattcgcgt accgcaggat
     1621 tacgttacgc aaagcggtcc gctgcgtgaa atgaatggtt cgctgggtgt gctggcgcaa
     1681 caactgcaaa atgcgaagtt gcaggccgat gctgcgcact ctgcgctaaa acagagcgat
     1741 gacctgaaac cggtatttga tcaggcattc accaaagtgg taacgacgcc agctgatgca
     1801 ttgcaaccgt tgattccggc ggcacaaact ttcacgcaac aactggtgat ggttggagac
     1861 tacattgctc agcaaggtac tcaggtgagc tttgtcgcaa atggcatcca gttcccgact
     1921 tcacagcagg caagtgaata taacaaactg attgcgccat taccggcaca acatcaggcg
     1981 tttaatcagg cctggactac agcagttact gcaacccaat aaagagtaaa agcccgagcg
     2041 attatcgggc tttacttcac gcagtcgcgg cttagtccgc gacgtgagga ttcacacagt
     2101 tcttctcaac ctttccttgt aacgcatcaa ttaaattatc cacggcacag gcggccatgc
     2161 cataacgcgt ctcatgggtg gcagatccaa tatgcggtac tgcgacgacg ttggccattg
     2221 agagcaacgg cgaatctacg gacagtggct cttgttcgaa gacatcaagc ccggcggcgt
     2281 gaatttcccc tttctgtaat gctgcgatca gtgcattttc gtcaaccacc gggccacgtc
     2341 cggcattaat gaaaatggcg gaggatttca ttttggcgaa ttgttctgcg ccaaacagat
     2401 gatgcgtctc atcagttaac ggcaggatca ggcaaacgaa atctgactct tgtaacagtg
     2461 tatccaaatc gcagtagcgg gcgttgaagc gttcttctgc ttctttatgg tggcggcgcg
     2521 cgttatagag gatgggcatg ttgaagccaa agtgcgcacg ttgtgccagc gccataccga
     2581 tccgtcccat cccgacaatg cccagtgttt tatggtgaac gtcagtgccg taccagtccg
     2641 ggcctatgct cgcggtccat tcgcctgctt ttacccgttc tgctacctcc acaacccgac
     2701 gagcggtaga caacaccagc gccatcagcg tatcggcgac ggtttctgtt aatacggttg
     2761 gcgtgtgcat cagcagaatt tttcgggcgg taagcgcatc gacatcaaaa ttgtcatagc
     2821 cgacggagat cgttgatgtg gcatgcagtt tcggcatttt ttccagcaat gcggcatcaa
     2881 cattctcgtt tgaacccagt aaaccttcag cttcggcaaa aattgctgca ttttgttcga
     2941 cggtttgtgg gctgaggttt gccacctggt gaacggtgaa atgctcttgc aggcgttgca
     3001 gtaaatcatc aggtaaggct ttgtagagga taacggacgg cttcatgctt ctctccattc
     3061 actgataagg cgatcacctt agcctgaaat atgatgaagg aaaactgaag aatgccaggt
     3121 gatttctgca tcacctggca tgaaagggat tacagcgggc ttaaggtaat ttctacacgg
     3181 cggttttgcg ccttaccttc tgcggtgctg ttgctggcga ttgggttagc cgggccaagg
     3241 ccctgagtac ggatgcggct ggcgtccacg ccctgggtga tcaacgcgct ggcaacggaa
     3301 tccgcacgct gctgggagag acgcatgttc aggtcgtgac cccccgtgct gtcggtataa
     3361 ccaatcacgt taaccgccgt tttcggatac tctgtcagca ccattgccac gccggtcagg
     3421 gtgttagcgc ctgccggttt cagggtcgcg ctgctgctgt cgaaggtcac attgttcggc
     3481 atattgagga taatgttatc cccgctgcgg gttacgctaa caccagtgcc gcgcattttg
     3541 tcacgcagct tcgcttcctg cacatccatg taataaccca cgccgccgcc cagagctgcg
     3601 cctgctgctg cgccaatcag cgcgccttta ccgcgatctt tcttcgaaga agagagcgca
     3661 ccaatacccg cgcccacgag agagcccaga cctgcgccga tagcagattt gcctgcttcg
     3721 cgttcgccgg tgtaagggtt agttgtgcag ccagataccg ccagagcgcc actcactacg
     3781 gcggcaataa gataaacacg tttcttcatt gttaatcctt aataaccttt ttattctttg
     3841 ccacgggttc cgtggcggga gattatgccg cgtgaacatg a
//
LOCUS       DAZPBM010000270         3833 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPBM010000270 DAZPBM010000000
VERSION     DAZPBM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.273"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /locus_tag="ACHUZQ_004153"
     CDS             complement(<1..266)
                     /locus_tag="ACHUZQ_004153"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1027691.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="ACHUZQ_004154"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="ACHUZQ_004154"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1027692.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="ACHUZQ_004155"
                     /pseudo
     CDS             complement(726..998)
                     /locus_tag="ACHUZQ_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHUZQ_004156"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHUZQ_004156"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1027693.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3734)
                     /locus_tag="ACHUZQ_004157"
     CDS             complement(2694..3734)
                     /locus_tag="ACHUZQ_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1027694.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT          999 a          932 c          916 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt acagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaagg cacaataact ttcgctatct tcgatacccc attgattctc taaagactcg
     3241 cgtcttttac tgatgatatc gatcgagtca aaaggaagca catgatattg gaaggtatct
     3301 ttaccaggtt caggctttcg cggccagaac tctagcgttt cagaccattg cttatgatag
     3361 aatcgataag gtgcgatcaa ttgtagcgcc tgtaacttct cgatactgag cggctcaata
     3421 cctttagcct gataataatg cagttgttct ttttttgctt taaaaccggc ccgaacaata
     3481 agccccatca taattaatag ataaagaaaa gagcatcccg cggtaatcag gcctctttca
     3541 ttcaaaccgt tggatgttat cgctgcgaac acaaacatta cagcgacaac acatgttaaa
     3601 taaaaccccc acttacaaag cagcatggcc ttattttctt taatcatccg ttcaaaatta
     3661 ctattaaata tttcccagcc attaaaagaa tacttctcgc tcccaggatg gttttgtaat
     3721 aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa ttttatccat
     3781 gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat atg
//
LOCUS       DAZPBM010000271         3828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPBM010000271 DAZPBM010000000
VERSION     DAZPBM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.274"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHUZQ_004158"
     CDS             <1..197
                     /locus_tag="ACHUZQ_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027695.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            208..375
                     /locus_tag="ACHUZQ_004159"
     CDS             208..375
                     /locus_tag="ACHUZQ_004159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027696.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            372..716
                     /locus_tag="ACHUZQ_004160"
     CDS             372..716
                     /locus_tag="ACHUZQ_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027697.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1008..1163
                     /gene="hokD"
                     /locus_tag="ACHUZQ_004161"
     CDS             1008..1163
                     /gene="hokD"
                     /locus_tag="ACHUZQ_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1027698.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1384..1672
                     /locus_tag="ACHUZQ_004162"
                     /pseudo
     CDS             1384..1672
                     /locus_tag="ACHUZQ_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1711..1989
                     /locus_tag="ACHUZQ_004163"
     CDS             1711..1989
                     /locus_tag="ACHUZQ_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027699.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1991..3049
                     /locus_tag="ACHUZQ_004164"
     CDS             1991..3049
                     /locus_tag="ACHUZQ_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1027700.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3050..3430
                     /locus_tag="ACHUZQ_004165"
     CDS             3050..3430
                     /locus_tag="ACHUZQ_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1027701.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3427..>3828
                     /locus_tag="ACHUZQ_004166"
     CDS             3427..>3828
                     /locus_tag="ACHUZQ_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1027702.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT         1034 a          853 c         1046 g          895 t
ORIGIN      
        1 catcaccgaa aaaaattggc ttttacacca ttggcgataa aagcatttac ctgtatgacc
       61 tacgccgcat ggatgaaatc atggaggctc ttgataatcg ttcgtcgatg gattggtgtg
      121 ttgctgttca tgatatgaat gcagggtttg atgaaaagat tttgttcccc tcatcagttg
      181 aaagcactgc gggttaagga gtaacacatg accactatta ccaaagaacg tattgaattg
      241 ttcattaaaa acccgcttga aaacgggctt acccgtggtg aacaaatgga actggcacgg
      301 attgcgctgg catcgctgga agcagagccg gttgtgttct ggtttgaaaa atatcaagaa
      361 ggggctacgg catgacgact tttaccagag agcagttaat agctcacgca gaggagacta
      421 ttgaagcaca gagactgtgc ataccgggca caatcgacca tgacatcatc cgcacatata
      481 agatggatat tgctgttctg gaaatcgcac tggtatcgct ggcagcagag ccagccggta
      541 aattgcatga atacaaacca gtgggatatc agcgtctggt cgatgagtta accatgctgg
      601 taaagcagtt aacctggcaa ctgagaaaag cgaagccaga ctgcaaatta ccggataagg
      661 cgatgagtta tctggagcgg aacggactga taagcgtgga ggatatttta cgatgacctg
      721 gcctgaagca ttaacaacgg taggaattgc gatggcggtg gcgctggtgg tgtattcgat
      781 ttgccgctgg ggataaaaac ggtttgcggg aaaaggagag ttaagtagaa ttgcagcggg
      841 tgcttgaggc tatctgtctc aggcatgaac accaaaaggc agatagagaa aagccccagt
      901 taacattacg cgtccggcaa gacgcttaac attaatctga ggctcaatct atgaacggca
      961 aatctaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa
     1021 aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa
     1081 cgaggaaaga cctctgcgag gtacgaattc gaaccggcca gacggaggtc gctgtcttca
     1141 cagcttacga acctgaggag taagagacca ggcgggggag aaatccctcg ccacctctga
     1201 tgggtcaggc atcctcaatg cacccacact taacccgctt cggcgggttt ttgtttttat
     1261 tttcaacgcg tttgaagttt tagatggtgc cggaatagaa tcaaaaatac ttaagtagcg
     1321 cgcagggaga agagggatgg accccgaaca ggggagtgct atttatctgg aaggattctg
     1381 ttgatgaaaa tcgaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
     1441 gttgagaatg gcgctattgt cagccactgc cgtattaaat gtttacagtc tcaacaaagg
     1501 aagagtggag ctgcgttaat tcattttgtg gatgggcttg tgacggatgg ttttatttcg
     1561 cgtgcaaatg aatttgtcac atcgttgccg tctctgaaag aagctgggat taaggctggt
     1621 ttttctgctt ttgaagatga gtgaattcat ctacaattca gcgcagggct gaacccctgt
     1681 tgagtaacac tgtgccaccg gagaaagccg atggcgcaaa attccagact acacaattct
     1741 gataattcag ccgtctttgc cagcaggcac gggcggcgct ctcacgcatt taaatctgat
     1801 tggtaccagc atcccccatg cactgaagaa caggccgaat ggctcattca gtgttaccgc
     1861 aggcgcggat gcgaggttaa aaaagccctt agcctcgact accgtcactg gataatctcc
     1921 gtcaggctcc cttactccga acggccaacg cgtccgtccc gcacattcca gcaacggatc
     1981 tggaggtaat gtgcgggtat tacttcgacc tgttctggta ccggaactcg gtctggttat
     2041 cgttaagcca ggccgtgaat caatgtcagc attccataac ggcagaatac tggtggagcc
     2101 ggaaccaaaa agcatgcgta atctgccgtc cggggtcgtt cctgccgctc gccagccgct
     2161 ggtggaagac aaaacattgc tgccgttttt cagtaacgca cgggtgattc gtgctgctgg
     2221 tggtgctggt gcattgtctg actggctgtt gcgccatatt aaatcctgcc agtggccaca
     2281 cggcgattat catcacagcg aaaccgtcat tcaccgttat ggtaccggcg caatggtgtt
     2341 gtgctggcac tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc
     2401 tcatcaaaac ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca
     2461 ggagcgtgaa ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga
     2521 cgcgctaccg gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaattcgctc
     2581 tgtgtacagt gaaagcgaca tcataccggg agagcagaca gccaccagca tactgaagca
     2641 gcgcacaaaa aatattgcgt tactgcctca cgtccaccag caacagaacc caccacagga
     2701 aaagacggtg gtcagcattg ccgttgatcc ggagtcaccg gctcagtatc tccagcgcca
     2761 gaaatcacaa cgggaagaga tgcctgtata cacgcgctgg gtaaaaacgc agaaatgcat
     2821 gacgtgtggc aatcaggcag atgatccgca tcacatcatt ggtcatggac tgggagggat
     2881 gggaacaaag gctgatgatt tgtttgttat tccgttatgc cgtaaatgcc gtaacgaact
     2941 acatgccgga gtaaaagatt ttgaagagaa acacggcagt cagctgttgt tgctgattcg
     3001 ttttttaatg cacgcgagaa attcgggtgt tctgaagtgg aaagcataaa tgaccgagcg
     3061 catagaattt gttttacctt acccgccgac ggtgaacact tactggcgtc gtcgtggcag
     3121 cacatatttt gtatcaaaag ccggggagcg ttatcgccgg gcagtggcgc ttattgttcg
     3181 ccagcagcgg ctgaaattaa gcctgtccgg aaggttggca ataaaaatta ttgcagaacc
     3241 accggataag cgccgccgtg acctggacaa tattctgaaa gcgccgctgg atgcgctgac
     3301 gcatgcgggg ttgctaatgg acgatgagca gtttgatgaa atcaatatcg ttcgtgctca
     3361 gccagtatct ggtggacgtc tgggggtgaa gatttatccc ataatgcttg aagggcaggt
     3421 caaaaaatga aactggaaga tttaccgaaa tactactccc caaaatcccc cggcctgact
     3481 gatgcatcgg cctcaacgtc gaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag
     3541 ggcatgacac agaatcgggc tgagatgggg gtttctgcgt tccttgggaa aatgggcatt
     3601 agtatgaatg acagagagcg ggcaacagaa ttgctgacag aatatgcact cagtcggtgt
     3661 gatcgcgtgg cggcgttaag aaaactcccg gcagaaataa aaccggcagt gatgcgtatt
     3721 atggcttcgt atgcgtttga agattatgcc cgtagcgcgg cgagcaaaaa acagtgcccc
     3781 tgctgtcacg gaaaaaaatt tattgaaagc gaggttttta caaacaag
//
LOCUS       DAZPBM010000272         3797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPBM010000272 DAZPBM010000000
VERSION     DAZPBM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.275"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHUZQ_004167"
     CDS             <1..81
                     /locus_tag="ACHUZQ_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027703.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHUZQ_004168"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHUZQ_004168"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1027704.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="ACHUZQ_004169"
     CDS             1027..1485
                     /locus_tag="ACHUZQ_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1027705.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="ACHUZQ_004170"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="ACHUZQ_004170"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1027706.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHUZQ_004171"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHUZQ_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1027707.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="ACHUZQ_004172"
     CDS             2691..3368
                     /locus_tag="ACHUZQ_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1027708.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHUZQ_004173"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHUZQ_004173"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1027709.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          903 c          990 g          953 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 tcacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAZPBM010000273         3755 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPBM010000273 DAZPBM010000000
VERSION     DAZPBM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.276"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUZQ_004174"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHUZQ_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1027710.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUZQ_004175"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHUZQ_004175"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR1027711.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHUZQ_004176"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHUZQ_004176"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR1027712.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          859 a          983 c          911 g         1002 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatggc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgttcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa ttaaa
//
LOCUS       DAZPBM010000274         3733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPBM010000274 DAZPBM010000000
VERSION     DAZPBM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.277"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHUZQ_004177"
     CDS             complement(<1..181)
                     /locus_tag="ACHUZQ_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR1027713.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="ACHUZQ_004178"
     CDS             425..682
                     /locus_tag="ACHUZQ_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR1027714.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHUZQ_004179"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHUZQ_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1027715.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHUZQ_004180"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHUZQ_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1027716.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHUZQ_004181"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHUZQ_004181"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1027717.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3733)
                     /locus_tag="ACHUZQ_004182"
     CDS             complement(3307..>3733)
                     /locus_tag="ACHUZQ_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1027718.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          952 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aat
//
LOCUS       DAZPBM010000275         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPBM010000275 DAZPBM010000000
VERSION     DAZPBM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.278"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHUZQ_004183"
     CDS             complement(89..652)
                     /locus_tag="ACHUZQ_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1027719.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHUZQ_004184"
     CDS             complement(756..974)
                     /locus_tag="ACHUZQ_004184"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027720.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHUZQ_004185"
     CDS             complement(1133..1519)
                     /locus_tag="ACHUZQ_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1027721.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHUZQ_004186"
     CDS             complement(1509..1880)
                     /locus_tag="ACHUZQ_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1027722.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHUZQ_004187"
     CDS             complement(1880..2137)
                     /locus_tag="ACHUZQ_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027723.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="ACHUZQ_004188"
     CDS             complement(2134..3525)
                     /locus_tag="ACHUZQ_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1027724.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="ACHUZQ_004189"
     CDS             complement(3522..>3627)
                     /locus_tag="ACHUZQ_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1027725.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAZPBM010000276         3609 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPBM010000276 DAZPBM010000000
VERSION     DAZPBM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.279"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(2..46)
                     /locus_tag="ACHUZQ_004190"
     CDS             complement(2..46)
                     /locus_tag="ACHUZQ_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027726.1"
                     /translation="MKRNDKILHYEGLN"
     gene            165..1889
                     /gene="ilvI"
                     /locus_tag="ACHUZQ_004191"
     CDS             165..1889
                     /gene="ilvI"
                     /locus_tag="ACHUZQ_004191"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1027727.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1892..2383
                     /gene="ilvN"
                     /locus_tag="ACHUZQ_004192"
     CDS             1892..2383
                     /gene="ilvN"
                     /locus_tag="ACHUZQ_004192"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1027728.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2395..2481
                     /locus_tag="ACHUZQ_004193"
     CDS             2395..2481
                     /locus_tag="ACHUZQ_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1027729.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2563..3567
                     /gene="cra"
                     /locus_tag="ACHUZQ_004194"
     CDS             2563..3567
                     /gene="cra"
                     /locus_tag="ACHUZQ_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1027730.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          841 a          864 c         1001 g          903 t
ORIGIN      
        1 atcaatttaa tccttcgtaa tggaggattt tatcgtttct tttcaccttt cctcctgttt
       61 attcttatta ccctgcgttt atgtctctgg ctgccaattg cttaagcaag atcggacggt
      121 taatgtgttt tacacatttt ttccgtcaaa cagtgaggca ggccatggag atgttgtctg
      181 gagccgagat ggtcgtccga tcgcttatcg atcagggcgt taaacaagta ttcggttatc
      241 ccggaggcgc agtccttgat atttacgatg cattgcatac cgtgggtggt atcgatcatg
      301 ttttagttcg tcatgagcag gcggcagtgc atatggccga tggtctggcg cgcgcgaccg
      361 gggaagtcgg cgtcgtgctg gtaacgtcgg gtccaggggc gaccaatgcg attactggca
      421 tcgccaccgc ttatatggat tccattccat tagttgtcct ttccgggcag gtagcgacct
      481 cgttgatagg ttacgatgcc tttcaggagt gcgacatggt ggggatttcg cgaccagtgg
      541 ttaaacacag ttttctggtt aagcaaacgg aagacattcc gcaggtgctg aaaaaggctt
      601 tctggctggc ggcaagtggt cgtcctggac cagtagtcgt tgatttaccg aaagatattc
      661 ttaatccggc gaacaaatta ccctatgtct ggccggagtc ggtcagtatg cgttcttaca
      721 atcccactac taccggacat aaagggcaaa ttaagcgcgc tctgcaaacg ctggtagcgg
      781 caaaaaaacc ggttgtctac gtaggcggtg gggcaatcac ggcgggctgc catcagcagt
      841 tgaaagaaac ggtggaggcg ttgaatctgc ccgttgtttc ctcattgatg gggctgggag
      901 cgtttccggc aacgcatcgt caggcactgg gcatgctggg aatgcacggt acctacgaag
      961 ccaatatgac gatgcataac gcggatgtga ttttcgccgt cggggtacga tttgatgacc
     1021 gaacgacgaa caatctggca aagtactgcc caaatgccac tgttctgcat atcgatattg
     1081 atcctacttc catttctaaa accgtgactg cggatatccc gattgtgggg gatgctcgcc
     1141 aggtcctcga acaaatgctt gaactcttgt cgcaagaatc cgcccatcaa ccactggatg
     1201 agatccgcga ctggtggcag caaattgaac agtggcgcgc tcgtcagtgc ctgaaatatg
     1261 acactcacag tgaaaagatt aaaccgcagg cggtgatcga gactctttgg cggttgacga
     1321 agggagacgc ttacgtgacg tccgatgtcg ggcagcacca gatgtttgct gcactttatt
     1381 atccattcga caaaccgcgt cgctggatca attccggtgg cctcggcacg atgggttttg
     1441 gtttacctgc ggcactgggc gtcaaaatgg cgttgccaga agaaaccgtg gtttgcgtca
     1501 ctggcgacgg cagtattcag atgaacatcc aggaactgtc taccgcgttg caatacgagt
     1561 tgcccgtact ggtggtgaat ctcaataacc gctatctggg gatggtgaag cagtggcagg
     1621 acatgatcta ttccggccgt cattcacaat cttatatgca atcgctaccc gatttcgtcc
     1681 gtctggcgga agcctatggt cacgtcggga tccagatttc tcatccgcat gagctggaaa
     1741 gcaaacttag cgaggcgctg gaacaggtgc acaataatcg cctggtgttt gttgatgtta
     1801 ccgtcgatgg cagcgagcac gtctacccga tgcagattcg cgggggcgga atggatgaaa
     1861 tgtggttaag caaaacggag agaacctgat tatgcgccgg atattatcag tcttactcga
     1921 aaatgaatca ggcgcgttat cccgcgtgat tggccttttt tcccagcgtg gctacaacat
     1981 tgaaagcctg accgttgcgt caaccgatga tccgacatta tcgcgtatga ccatccagac
     2041 cgtgggcgat gaaaaagtac ttgagcagat cgaaaagcaa ttacacaagc tggtcgatgt
     2101 cttgcgcgtg agtgagttgg ggcagggcgc gcatgttgag cgggaaatca tgctggtgaa
     2161 aattcaggcc agcggttacg ggcgtgacga agtgaaacgt aatacggaaa tattccgtgg
     2221 gcaaattatc gatgtcacac cctcgcttta taccgttcaa ttagcaggca ccagcgataa
     2281 gcttgatgca tttttagcat cgattcgcga ggtggcgaaa attgtggaag ttgctcgttc
     2341 tggtgtggtc ggactttcgc gcggcgataa aataatgcgt tgagaatgat ctgaatgcgc
     2401 aatttgcagc ccaacatgtc acgttgggct ttttttgcga aatcagtggg aacctggaat
     2461 aaaagcagtt gccgcagtta attttatgcg cttagatgtt aatgaattta acccatacca
     2521 gtacaatggc tatggttttt acattttacg caaggggcaa ttgtgaaact ggatgaaatc
     2581 gctcggctgg cgggagtgtc gcggaccact gcaagctatg ttattaacgg caaagcgaag
     2641 caataccgtg tgagcgacaa aaccgttgaa aaagtcatgg ctgtggtgcg tgagcacaat
     2701 taccacccga acgccgtggc agctgggctt cgtgctggac gcacacgttc tattggtctt
     2761 gtgatccccg atctggagaa caccagctat acccgcatcg ctaactatct tgaacgccag
     2821 gcgcggcaac ggggttatca actgctaatt gcctgctcag aagatcagcc agacaacgaa
     2881 atgcgctgta ttgagcacct tttacagcgt caggtcgatg ccattattgt ttcgacgtcg
     2941 ttgcctcctg agcatccttt ttatcaacgc tgggctaacg acccgttccc gattgtcgcg
     3001 ctggaccgcg ccctcgatcg tgaacacttc accagcgtgg ttggtgccga tcaggatgat
     3061 gccgaaatgc tggcggaaga gttacgtaag tttcccgccg agacggtgct ttatcttggc
     3121 gcgctgccgg agctttctgt cagcttcctg cgtgaacaag gtttccgtac tgcctggaaa
     3181 gatgatccgc gcgaagtgca tttcctgtat gccaacagct atgagcggga ggtggctgcc
     3241 cagttattcg aaaaatggct ggaaacgcat ccgatgccgc aggcgctgtt cacaacgtcg
     3301 tttgcgttgt tgcaaggggt gatggatgtc acgctacgtc gcgatggtaa actgccttct
     3361 gacctggcaa ttgccacctt tggcgataac gaactgctcg acttcttaca gtgcccggtg
     3421 ctggcagtgg ctcaacgtca ccgcgatgtc gcagagcgtg tgctggagat tgtcctggca
     3481 agcctggacg aaccgcgtaa gccaaaacct ggtttaacgc gcattaaacg taatctctat
     3541 cgccgcggtg tgctcagccg tagctaagcc gcgaacaaaa atacgcgcca ggtgaatttc
     3601 cctctggcg
//
LOCUS       DAZPBM010000277         3560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPBM010000277 DAZPBM010000000
VERSION     DAZPBM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.280"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHUZQ_004195"
     CDS             129..1376
                     /locus_tag="ACHUZQ_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR1027731.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUZQ_004196"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHUZQ_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR1027732.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHUZQ_004197"
     CDS             complement(2228..2572)
                     /locus_tag="ACHUZQ_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027733.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHUZQ_004198"
     CDS             2814..3416
                     /locus_tag="ACHUZQ_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027734.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          887 a          715 c          816 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct aggattgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttaaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggggagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgtc
//
LOCUS       DAZPBM010000278         3554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPBM010000278 DAZPBM010000000
VERSION     DAZPBM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.281"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(181..441)
                     /gene="yfhL"
                     /locus_tag="ACHUZQ_004199"
     CDS             complement(181..441)
                     /gene="yfhL"
                     /locus_tag="ACHUZQ_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1027735.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            656..1846
                     /locus_tag="ACHUZQ_004200"
                     /pseudo
     CDS             656..1846
                     /locus_tag="ACHUZQ_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2031..3218)
                     /locus_tag="ACHUZQ_004201"
     CDS             complement(2031..3218)
                     /locus_tag="ACHUZQ_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1027736.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(3218..>3554)
                     /locus_tag="ACHUZQ_004202"
     CDS             complement(3218..>3554)
                     /locus_tag="ACHUZQ_004202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027737.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT          907 a          947 c          827 g          873 t
ORIGIN      
        1 acttaaggtg cgtccggtga accaatcgga cgcgccttta ataactataa ataagtgtct
       61 tggcagatac tatataaatt aacttagtga atgattatgc taatgtcatc aattaaataa
      121 atataatggc gttaaggctt cccagtaata taattaatac tctacttcca gagtagaata
      181 ttaaatttta tccgcgtggt gcatcagcac aaatttatcc cacaactgtt cttctgtctc
      241 gacatgcgcc ggatctttca caatagtatt ggggatcggg cacaccttct ggcaggttgg
      301 tgtctcgtag tgccctacgc attcggtaca cttatcgctg ttaatctcgt agatatgatc
      361 tcccattgaa atcgcctcat tcgggcattc gggttcacac atatcacaat taatacagcg
      421 tttagtaatt agtaaagaca tttcaatgga ttaccgttaa atcattttaa aatcagtaag
      481 ttgtatcgag tttgtatgct ttactgtcat taacttactg tatgttgatc cagtgtattt
      541 aaccttgata aactcagtcc agcaacacaa aaccgcaaca cattgcattt tgtcccgtag
      601 aaaagacttg tatgtgtgag cttgttttct gcgcctacgc agataaggat tgagaatgcc
      661 gcgcactgta acacataatc cggatagccc caataatgac gatgttttag ccgcatctga
      721 aaaatgggac gcctgtaaac ccccctatac cagcgcacac atgaaaatct gtgttgctgc
      781 cgccaaaatc atcctcgctg cttccggcgt ggctcgccgt tccaaatacg aaaaagagaa
      841 ctatctccgt atcgatttca gcaaagccgg taaggttaca ttttacgccg agtttccaaa
      901 aaagatgggc ctcaaaggta aaaagctcgg cgagtggccg gagctcgcta tccagctggc
      961 gcgcgaaaaa gcactaggta tggctgacgg tggactgcgg gcagagtccg tacatgcagc
     1021 gctggaaatg taccgggatg acctcaaagc caaagtagcc cggcagaagc tgagcccgga
     1081 cagtttcaca acctacgggg tgcgtatcga ccggattaaa gcaacgttcg gcgagcgtga
     1141 ggtgttcagc gacgtaacat acaatcggct ggtggaagtg ctggacgagt ggatcgccac
     1201 tcgctcgaac aataacgccc tggagttgtt tgccgagctc cgtcggttct ggaagttctg
     1261 cgcacctact ctttgcaacg gccgcaatgt tgccgccagt ctgccagatg attatgtttc
     1321 ctcccgcgta cagaaaccta cccccacacg gctttttacc gatattgaat caatcgcccg
     1381 actctggctc aatgttgctg cctgcacctc tgtacaccag aagaatgctg ttcgcttcat
     1441 gatcatcact ggtgttcgtc cgattaatgt ccataacctg cgctgggact acgtttacga
     1501 ggaggctggt gaaattgttt atccggaagg ggttatcggc atgcgagggg ctatgaaaac
     1561 acaaaaggct ttccgcctgc cgataacgcc tgagatccgg cggattatcg acgagcagaa
     1621 agcctggcgt gattcagttc ctgagtgcaa cagggattat gtatttttgc agccacgtga
     1681 tccaatgcag ccattttcaa aacgatcact ggataagctg gtgaaaacat acagcccgga
     1741 cggggctgta aaaggaataa aacatgatgg gactgttaaa gggaaagacg gtgcatttaa
     1801 tacgatgtgc cgtaaattcc ttaagagcaa tgttattgcc ttgatcgata ggaattaaaa
     1861 ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc ttcaacacca cttttgctca
     1921 tatgaacgga accgacgatt aaactggatg gctctgattg attcagggta tgaatggcgg
     1981 ttttttgctc cgtttccctc aaaatggacg caactttccc tctgcggctc tcagccgcac
     2041 caccgcatcc gggccagcgg ctcatgcatc aggacctgct ctgccagacg gtagccccgc
     2101 ttcagccccg taaaacgcat ctgactcccg cacagcacgc acttcagcgg gtcaaccttc
     2161 agtaacctct gatacatccc tctccaggtg atttgcatcg ccgtttttct cactgtctcc
     2221 gttatgatgt acaccacttc ttccagtaac cgccgtttcg ccggactcaa aaaaccgtag
     2281 tacctcacca tacggaaccc cttatccgcc acatgccagg agaacctttc catgaactca
     2341 tctccactca tcaacaggta ttcttcccgt tttgttcggt gactgttgta acgcagaccg
     2401 atttcatcct gaccggcata atgctccaga cgactcatcg gcaccggtgg ctttttcagg
     2461 taagagccaa agtacaccgc cacatggggg gcattatcca tcacccggga tacgttgaca
     2521 ttccagccac ggcggtaatg cgtgtccagg aagcgattcc attcccgttt actgcttcct
     2581 tctgctgcca gcgcatccgg catcaccagg tcagggtatt tccgtgacag caaccgtgtt
     2641 atccggtagc gccacatgct catcacctta cgggcgtaaa aatgaagatt tttccaggtg
     2701 tggcccgacg tcacaccacc ggcagttgtc gataaatgga tatgcggatg ccactgctgg
     2761 tcacgccccc atgtgtggat caccgtgaat atccccggct ccacatctgc ctgatggcag
     2821 atttccagta tcacatccgc tgcaatgcgg ctcatctctg tcagtaacca ccggttgtgg
     2881 aacaccaggg accagtactg gcagggaagt gtgaacacaa tatgctgcca cgggcagtcg
     2941 gggaccaggc tcagcagata ctgtatccac tgtgcgccag ccttcacccc gcagtgcggg
     3001 caggagcggc ttttacaccg gaagcagacc ttttttgtat ggcaacagtc cggtgatgaa
     3061 cagcaccact gtgtataccc catcagtgtg gtcccgcacg ccatgatttt ggtcaccgac
     3121 tcaatcacca ccggacgtac tgccctttcc ggctgcttct ccagccagtt aagccagtgg
     3181 tttccctgct gaaagatatc ggcaaaacgg ggaagcatca gaagggcggg gcgactccgt
     3241 ccggccagtg aaccgtgcca cactccgggc agtacatacc gccggcgctg ataccggaaa
     3301 gaatggtcgc aaattcccgc tccgtgcagc gggcgatttc cggataccct tcgtcatcaa
     3361 cacgtacaaa ccagaagacc agctttttgt ttcccgcatc cacaaagaac ggaatattca
     3421 ggtctgcgca gcattcaacg gcatcgtcaa aactatcaaa gcgcagaact tctgcgtctt
     3481 cttcgtcaaa aaaatcatct tcgtgaagct tcacgacata gcggggaagt ttgcttcttt
     3541 gagaggcggg ttta
//
LOCUS       DAZPBM010000279         3543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPBM010000279 DAZPBM010000000
VERSION     DAZPBM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.282"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUZQ_004203"
     CDS             complement(<1..140)
                     /locus_tag="ACHUZQ_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1027738.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUZQ_004204"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUZQ_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1027739.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHUZQ_004205"
     CDS             complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHUZQ_004205"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR1027740.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUZQ_004206"
     CDS             complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUZQ_004206"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR1027741.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          925 a          982 c          783 g          853 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta
      901 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
      961 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     1021 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     1081 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     1141 cgctcgccgg aaagataagg cagaaaccaa actggctcga cactttcatc agcctgttga
     1201 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     1261 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     1321 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     1381 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     1441 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     1501 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     1561 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     1621 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     1681 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     1741 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     1801 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     1861 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     1921 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     1981 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga
     2041 tgcgggcgcg aaacggtcag cttttccgtt tgcgaatcaa ccacctcacc ctgctcgttg
     2101 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     2161 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     2221 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     2281 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     2341 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     2401 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     2461 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     2521 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     2581 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     2641 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     2701 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     2761 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     2821 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aagccgattt
     2881 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     2941 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     3001 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     3061 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     3121 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     3181 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     3241 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     3301 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     3361 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     3421 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     3481 acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt
     3541 gca
//
LOCUS       DAZPBM010000280         3533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPBM010000280 DAZPBM010000000
VERSION     DAZPBM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.283"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..638)
                     /locus_tag="ACHUZQ_004207"
     CDS             complement(<1..638)
                     /locus_tag="ACHUZQ_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1027742.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
     gene            complement(645..953)
                     /locus_tag="ACHUZQ_004208"
                     /pseudo
     CDS             complement(645..953)
                     /locus_tag="ACHUZQ_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1332..1844)
                     /locus_tag="ACHUZQ_004209"
                     /pseudo
     CDS             complement(1332..1844)
                     /locus_tag="ACHUZQ_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(1857..2279)
                     /locus_tag="ACHUZQ_004210"
     CDS             complement(1857..2279)
                     /locus_tag="ACHUZQ_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1027743.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2276..2518)
                     /locus_tag="ACHUZQ_004211"
     CDS             complement(2276..2518)
                     /locus_tag="ACHUZQ_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1027744.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            2615..3034
                     /locus_tag="ACHUZQ_004212"
     CDS             2615..3034
                     /locus_tag="ACHUZQ_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1027745.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3340..3495
                     /locus_tag="ACHUZQ_004213"
     CDS             3340..3495
                     /locus_tag="ACHUZQ_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1027746.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          893 a          928 c          794 g          918 t
ORIGIN      
        1 agtgtattca tcgctgcatg gttaatattt gtcagcatcc ccacaccgcg taacgatgcc
       61 gtccggcgat caacaatctg gttcagtacc acctgctcgt ttttcgtatc ccgctgcatg
      121 ccaatttcat ccaggaccag caggtcaaca ccacaaagct cctgtaaaaa tttttcaccg
      181 gatttgccgt tgtcgtagct ctcatgcaac acgctcatga catcggacac ggtgacgata
      241 atcacgctac gccccttcgc catcagccgg ttgccaatcg cgtctgcaag atgatttttt
      301 ccggtgccgg gcttaccgct gaacacaaaa ttcgtacagc ctgtcatcag ttcgtcagct
      361 atggatttcg cctgactcag tgcatgtcgc tgaccgtcgt tctgcgcccg gtaattcgca
      421 aacgaacact tccggtgcaa cggctggatg ccggagcggt tcaggatttt ttccacccgc
      481 aactgacgat tcaggcggtt gatctcctcg caacgtttct ggccttcagc aagttgccac
      541 tcgcgccact ccgcaaccgt tctgaatggg gcggttacat gtggtggggt cagtctgcgg
      601 atacgctcca gaacgcctcc tgtcgcaata tttttcatgg tctgttaccc cctgaagcct
      661 ggcgggatcg cactgtccgg caacgagacg gtgttaacct gtcggagcaa cgtctcaggc
      721 cgaacacctt tcggcgcgaa cagaccctgg tattcattgg cgatgctgtg tcgaatcacc
      781 tgctcaggtg taaaaccctg ctgacggaat ttttccagtt cccgtatcgc cccgttagcg
      841 ccctgctccg ttcgaatcgg ttttcgcaat gcctgcctga actggaccca ctcatgccag
      901 agtgtttctg gcaaccagtc gggcagatca actgacaacg ggtcaaattt tttctggcga
      961 acagccccac cgataaccag aattccctgc tcggtgtgcc ttgcgatatc actgcgccgc
     1021 aagacacata tcccggtttc aacagcaccg attttgagca gtgatttctg tcgcggagaa
     1081 tttcggtcaa gctcgctccc acaccggaca ccgttcctgt cagtcacgac gatatcaatc
     1141 cgccctccgc aaccgtcacc acgctctggc accggatact cacgacagac atccaggcca
     1201 gcagcctgca acgcaaccac cgcagaatta caaaattccg ctgcagtacc gccagataat
     1261 ctcccctcaa gcactccagc cacaaaatcc cgaaactccc ttccggaagg gaagggtttg
     1321 ggatgggtta gatctgtttt tagatcttta tctgtatctt tattagttgc ctttgtgttg
     1381 gcatcatgtt cgaacatcac tccaacatct gtttgaacac ctgttaaatt tctctcttgt
     1441 tttgtttgaa catctgcttc ctttctgctt cttctggcct gaacagatgc ttttcctgcg
     1501 gctgattttt tggttaattt ttctctgaca gatgccagat cttcctcaat ccgaagatgc
     1561 atccattcgt cgccgttatc acaaaaaaac tcccgcaagg atggttcaac atcaacccat
     1621 cgctcgttag tcagacgggc aatttttgcc aacctgtttt tgggtattgg ctttcctgtt
     1681 tgccagtaat tgaacatcag caacaaatac gcgccgtgct cctctgctga caaatgcatg
     1741 gtgtcagcca ggtaatcagc tatgtacagt tgcatgtatg gtaatgcggc cataattgcc
     1801 ccgtatgatg ctgcccggtt gcttagaata agcacaaaca gcatggaaac ttttgcttaa
     1861 tgaacaatga cagaatcgtc ggaagacccg ccgccgctga aatgcgcttt ccggtaaacg
     1921 gcctggactg catcatcatg cgcatcaatt gccgtactca acgcttcctg cgccgccagt
     1981 aatgcacggc gttccagggt atcgaagatg cagagtcggt gacgcagctc gcgcggaaga
     2041 attgccagaa ccgcagggat cagtttctga attttttccc tttgcgcttt cgtttcacct
     2101 ttcaaccaac ggtgatagat attctgctga ttgttccagt ccttgcctgg tacaaggggc
     2161 aattcgccgc ccccctggcg cagatattct tcagtaattg cgttagcgac ccacgcctgc
     2221 cctttttcgg ctgccagggc taacaacact gattcgatgt gctcatgcct gattttcatg
     2281 aatcaaccgc tcctatgctg ttttcgctat gcttaccgtc tggggggaat acatcgtcaa
     2341 gtccacaatg agcgccaagc cgattaaggg tagaaacaat ttttctgcac tcctctagtc
     2401 caggggtacg aaaatttgct tcgtaatttg ccagtcggct ttgtatccac cctaactgaa
     2461 cagcaagttg tctttgagac agcccaagct gttttcgata tgttgaaatt ttgttcattg
     2521 aaaacctccg atgacaattt taaacacacc ttgtgttata tagccaagct gttttgtgtt
     2581 ttatgtaaat cacgattcgt gatacaagga tgcaatggaa aaagaaaacg aaaaaattgc
     2641 cgctagtagg ctcaatgaca aaattgcaat gcgtcttaaa gagcgcaggc agaagcttgg
     2701 tttatctcaa ggaaaacttg ctgaaatctg cggatggacg caatcgcgta taggtaacta
     2761 tgaggcgggc agcagaaatg ttggagtaca tgacgctgtc gtattgggaa aggcacttgg
     2821 catatctcct cctgagctcc tctttggaga acaggaatct tctgaattgt ggttaaatga
     2881 atcccaacga aaacttcttg agttgtttaa ccagctaccg ggctcagaac aacaacgaat
     2941 gattgagcta tttgaagtcc ggctaaaaga aatcgatgag tatgtagaaa aatatttgag
     3001 aggcaggatt aaagataatc ccccaccgga ataatgatct tgctatcaca gtaatatgcc
     3061 aatcagcccg ctatcagcgg gcttttttgt agcatcatca tatgacactc accataaaac
     3121 acattgcgtg ttgacacaag aaaacaaatt gtgtttaata agcatatcca aacaacgccc
     3181 caccagagaa cggcaggaca atacctcgag ttatccagtc actgaacagg gctaagtagc
     3241 cagcctgagg catacgaaca tgacggcagt tgttgattga tacaaagcgc agtagataaa
     3301 acgttccgcc acccggcgtt aaggggaaat ggggtcaaca tgaatactat cgatcttggc
     3361 aacaacgaat ctctggtatg tggcgtgttt cccaatcaag acggcacatt caccgccatg
     3421 acgtatacca aaagcaaaac gtttaaaacc gaagctggcg cacgtcgttg gttgggaaga
     3481 cattcaggtg agtaaaatga acgagacaga attaaaacac gttatcgctc tac
//
LOCUS       DAZPBM010000281         3405 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPBM010000281 DAZPBM010000000
VERSION     DAZPBM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.284"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUZQ_004214"
     CDS             <1..784
                     /locus_tag="ACHUZQ_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1027747.1"
                     /translation="FSVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSL
                     HREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQ
                     LQTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVI
                     NINSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSS
                     EDHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            781..1686
                     /locus_tag="ACHUZQ_004215"
     CDS             781..1686
                     /locus_tag="ACHUZQ_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1027748.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1683..2753
                     /locus_tag="ACHUZQ_004216"
     CDS             1683..2753
                     /locus_tag="ACHUZQ_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR1027749.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2990..3343
                     /locus_tag="ACHUZQ_004217"
     CDS             2990..3343
                     /locus_tag="ACHUZQ_004217"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027750.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
BASE COUNT          905 a          778 c          935 g          787 t
ORIGIN      
        1 gtttagtgtc agtgccccgg gccatcattc atggaaacga gactttgatc cggacagccc
       61 cgagataaaa ttcagtgatc gcagggaagc ccttaaactg atgacgcaaa tcgaatcgac
      121 cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg cctgaactgg aaaaaatcgt
      181 caggggtatc gaaacaggtt ttcgtggtac ggccctgtat gccacagaaa atattgccgg
      241 acgtatcaat gcccgcctgg aggacgaggg cttcactgta aaaatcactt tcccggcagt
      301 cagccagtta cagaccagga tcgcggtaaa aacaaatctg agtgcgctta tggaggaaag
      361 aactgagaca gtcacccgtc gccgtcggca gagtggtttc tggggaaaga tttgtggagc
      421 gtttggcacc agtgactggg gctgggaaaa ctataaggag aatgtgagcc gcagtgtgat
      481 caatatcaac tcggtcagga aggaagtgat gtcactgacc cgggcatatt tcggggagtt
      541 gcaggcatcc attgagcaga atattaacca gcccgtccgc caggagatcg atgatttttt
      601 ctgtacattc agggaaaaag tcgaacagct acgtaacaca ctcattcaga gctctgaaga
      661 tcataaacgc gatcagcagg cacaggagca gcttacggag cgccttcagg cattaaatga
      721 aagggttccc gagctgatta ctgacagtaa ggcgttaagg gaagaactgg agacactgct
      781 gtgacctcac catttattca acaaattgct gataaccggg tatgtcaggt actcagttgc
      841 cttcctgaaa aatttgtggt tgattttgca aacggtattg atgttgcaca ggagcataac
      901 cgcacggccg gggggcgcac gtttttccgg cgtttaaaag aaggtctgac tggcaaaggc
      961 gctgtacgcc agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc
     1021 tggctgacgg agctgacaac gtcgcttgcc accacaaatt acgcgattac ccgggtaaac
     1081 gacagggtca gttcactggt cagtgatact gccaggctgg cgcattattc ggcagacacg
     1141 cgggagcagt tactcaccct ggcagaacag gttcatcaga aactgaatca tcttgaagaa
     1201 aaactccacc gtgttgacca ggttcagcgg gcacagttac atcttgagca gatattctca
     1261 tggtggagcg ccgggcgata cgcgtcattt tcccctgccg gacgttgtta tgtggcgctt
     1321 gaagagcttc gctggggcgc gtttggtgat gtgatacgtc agggtgaaac aggccaggtt
     1381 aaccagttac tggatatcct cagacataaa gcattaacgc aaatggcaca ggagaatggc
     1441 ggtagcgcaa cagtacgtct gaacacactg gactggcttg gtggtcagag ccgggaacag
     1501 gctgataacg aatggcatga agcggttaac tggctggggg actggtgcag tgaagagcgg
     1561 catcctgtga tctggtcaac cacacaggct gcagaacact taccggttcg tatgccccgt
     1621 ctctgctctg cagaacgcct ctctgaaagt atggttgatg aaatatttca gaagggggag
     1681 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg cggcggggcg gtgggcgctt
     1741 atcaggcggg agtggttaag gcactggcag agtgcggcgc acagatcagt atggtctcag
     1801 gagccagtat cggggctctc aatggtgcca ttatcacggc ctctcccgat ctgtcagaag
     1861 ccgccttacg cctggaggcg ctctgggatc atctggggaa taatcaggtt ctgtcggtaa
     1921 acagatcggt ttacttttca ttgctgaaaa aactggttca ggccatgaac ctctgccaga
     1981 tccccggacg tgcgggcgca ctgcttacga cgctttttcg ccatatatcg acaatcaacg
     2041 ggttcgacaa tccgatgatt cagccgctgt tgtcagatga gcccctgaca gcgctgatgg
     2101 atcattatct tgatacagat gctctggccg acgggctacc gctgtacgtg tcgctgtacc
     2161 ccacagaagg aggcatgcag gatattattg actgcattcg tgctgaactg ggtgccggaa
     2221 ccacgaaaaa cgctgttttt cagcatatcc agagtctgcc ccgcgggcag cagaaagaag
     2281 ccttactggc gtcagccgcg ctgcctctgc tgttccgtcc ccgcgaggtt cagggaacaa
     2341 tgtacggtga tggtgggatg ggcggatggc gaaacaggca ggggaatact cccgtgacgc
     2401 cactggtgga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     2461 gggatcgtcg ggcttatccg gacaccacaa tccttgaaat ccgtccccgg aaaaggttga
     2521 aacaaatcgg ggacgaaggc aaaagtggcg gtctgctcag ttttacatcg gcacataccg
     2581 acgcctggcg tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac
     2641 cgctggcagc acgtcaggca ctgacccggt cagagacggt attgcagaaa agcctggaga
     2701 taactgaagg tgcagattcg gcactgagaa acgcgatggc ccggattaaa taaagacgct
     2761 ccggagaaac gccaccgcca ctgtggtggt gtttttcctg tctggtatta ttgaaaatat
     2821 tttcagatat atattacctg gatgatggtt aagattaatt tatttaattt tggggagaaa
     2881 actcatgaaa ctgttaaaca ttcttttagc tgttgtttta actgtaagtg caaccgtagc
     2941 acaaacagaa tcggcattgt agacgcgtaa cagtattgct ggcactgata tgttcagtat
     3001 cggtaatgtt tctattaact gcacgattaa tccagaagac aacaatttgc taccacacta
     3061 tgtgtggatt tacggtcata cctccaccca gctaaatcag tacgattctg atattatttt
     3121 tatcatcaac gataaaaagt atccggcccc atctgtcgac ggaacacgaa tgaataagaa
     3181 tgcatgggta tactttatcg atgctatcag tgaagccacg caattcgacg ttttagtaaa
     3241 cggtaagaaa gtcgacagct ataccgcaaa tatcaaaaat gtgaaaaaga ctttgggtaa
     3301 caaattctac gacagctgct ggaacacctg gttccaggaa taattgataa cgtggtgtaa
     3361 aacacctcag tttaatttaa tacaatgagt gaaaatgaat attat
//
LOCUS       DAZPBM010000282         3384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPBM010000282 DAZPBM010000000
VERSION     DAZPBM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.285"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHUZQ_004218"
     CDS             complement(<1..1361)
                     /locus_tag="ACHUZQ_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1027751.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHUZQ_004219"
     CDS             complement(1426..1545)
                     /locus_tag="ACHUZQ_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1027752.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHUZQ_004220"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHUZQ_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..>3384)
                     /locus_tag="ACHUZQ_004221"
     CDS             complement(2272..>3384)
                     /locus_tag="ACHUZQ_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR1027753.1"
                     /translation="DLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNEQTLFDN
                     IRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQVKGHYGD
                     KFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNGNCSERL
                     FNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPEVKLSGE
                     NLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNYDIQYVN
                     WGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDI
                     LFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT          932 a          764 c          614 g         1074 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cacctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatc
//
LOCUS       DAZPBM010000283         3323 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPBM010000283 DAZPBM010000000
VERSION     DAZPBM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.286"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="ACHUZQ_004222"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="ACHUZQ_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1027754.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /gene="fliA"
                     /locus_tag="ACHUZQ_004223"
     CDS             complement(367..1086)
                     /gene="fliA"
                     /locus_tag="ACHUZQ_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR1027755.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENQLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..2825)
                     /locus_tag="ACHUZQ_004224"
     CDS             complement(1251..2825)
                     /locus_tag="ACHUZQ_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR1027756.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3080..3323
                     /locus_tag="ACHUZQ_004225"
                     /pseudo
     CDS             3080..3323
                     /locus_tag="ACHUZQ_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          852 a          790 c          712 g          969 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc tggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actgagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tggacatcta ggttttttcc cccaaaataa acagacattg gtgcatcatt ataagctaat
     1861 gctttaccca ttttacccgc agctaaatca actgatgtgg cagaaccatt atcttcaaca
     1921 aatgcagtta cacgaccacc aaagtttgct aactgaacat ccttaccacc cacctgaact
     1981 ttttcataac ctgtagagtt ttgtttcaat aatgtaaaac cgttcgtgtt ggtattatta
     2041 tcttgaccaa caaaataagt ctgcccttcc acctgtactt gataaacttt tgttcctgca
     2101 gtaccggtcg catcagttgt atcctttgta tcttgtacat taccggtacc aaaataagtt
     2161 tcaacatcag catttttata attgctaccg aatgctttag cacctcccac actaccatca
     2221 gttgcattta ccttagttaa gtcaggagcg gcttcgctct taagtgcaac cgcagaggac
     2281 attggaactt ttcctagtgc tacgctaaag ttatcaagcc ccaaggtttt agcatcaatt
     2341 ttatcaaggg caatctcaat ggtttcacca tcgttcgcac ctacttgaat tttcatagtc
     2401 tgatcagaag ccagaacttt tacaccatta aattgtgtct ggttagaaac acgatcaatt
     2461 tcactaaggc gttgtgtaat ttcatcctga attgaattca ggtcggagtc agagtttgta
     2521 ccattagtgg cctgtactga caattcacga atacgctgta agttattgtt gatttcagaa
     2581 agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg ggaagcctga
     2641 gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc tgcgtcatct
     2701 ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct cagagaagac
     2761 tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt aatgacttgt
     2821 gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc ccacggtttc
     2881 tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt agtttctctc
     2941 ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc gcatcatctt
     3001 cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat ctactgtttt
     3061 caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt cgaatctgcc
     3121 gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa acccaattac
     3181 tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa aaatgtgcat
     3241 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     3301 ccgttaccag tagcaatgaa gac
//
LOCUS       DAZPBM010000284         3313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPBM010000284 DAZPBM010000000
VERSION     DAZPBM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.287"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..602)
                     /locus_tag="ACHUZQ_004226"
     CDS             complement(<1..602)
                     /locus_tag="ACHUZQ_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1027757.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1122..2021
                     /locus_tag="ACHUZQ_004227"
     CDS             1122..2021
                     /locus_tag="ACHUZQ_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1027758.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHUZQ_004228"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="ACHUZQ_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1027759.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
BASE COUNT          838 a          856 c          778 g          841 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcacgaggc
      301 acaatggcca gcacattaaa atgaataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactggcc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga ttttcattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actgaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatggt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cagcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttat ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagt acctggactg accgactata ctttttaaga
     2041 atgaccacaa cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct gattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acgctagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggctgt ggcgctttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gcgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctacg tgattttatt tcgctgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcggt
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttaccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcattcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaa
//
LOCUS       DAZPBM010000285         3284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPBM010000285 DAZPBM010000000
VERSION     DAZPBM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.288"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..507
                     /locus_tag="ACHUZQ_004229"
     CDS             <1..507
                     /locus_tag="ACHUZQ_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1027760.1"
                     /translation="KIHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNF
                     NNKILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDT
                     IDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTL
                     SYREVNRD"
     gene            500..982
                     /gene="yqeJ"
                     /locus_tag="ACHUZQ_004230"
     CDS             500..982
                     /gene="yqeJ"
                     /locus_tag="ACHUZQ_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR1027761.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1016..1096)
                     /locus_tag="ACHUZQ_004231"
     CDS             complement(1016..1096)
                     /locus_tag="ACHUZQ_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027762.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHUZQ_004232"
                     /pseudo
     CDS             complement(1132..1556)
                     /gene="yqeK"
                     /locus_tag="ACHUZQ_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1986..2476
                     /locus_tag="ACHUZQ_004233"
                     /pseudo
     CDS             1986..2476
                     /locus_tag="ACHUZQ_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2701..3192
                     /locus_tag="ACHUZQ_004234"
     CDS             2701..3192
                     /locus_tag="ACHUZQ_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1027763.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT         1241 a          528 c          528 g          987 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa
      601 cgaaacatgc actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat
      661 cgactttatg tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca
      721 acaaggtaac agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa
      781 ctatgacaca gcccatttaa catcaaagaa agttaataag tttgagatta tggatcaagt
      841 tgatgatgat agacttgcac aacttattcc tgatttttat gtctttccag aaaaaagtgt
      901 aagctataat atactaaagc aaggtaagca tgcttttatt ttgagcattg gtaacagagc
      961 aataatgcat tgtgcaaggt aaactaaaat agataacaaa aaagatggaa cctcgttaat
     1021 cataaaacaa taaaattaaa tattctcgca gtatatggca gtctaaagca tcaaagtttt
     1081 gatcaacatc tttcatttta gacatctcct tgcaatgtaa aatatcatgt atcaacaaca
     1141 tctggtgcaa atccatgagt cgaactcgac attcatctta gctcgattat tattccttcg
     1201 tacagtcgat gtaaacaata cagaaagagg attattaaga accagtttac tcaggaatca
     1261 atagaggaaa ggactaacgt ttctttaaaa gaattgattt catcatctgt taaactaaac
     1321 tcatcattga cagatcgtga gatataactg tttttaactt tactctttac gttgacttta
     1381 ttgacagaat taacattcac atatcttgaa tttaatgtcc atgatgttgt ttgcgtgaga
     1441 acattctcag cattaaggaa tttttttaac ggaatccttg gtttcttttt cgagtccgaa
     1501 tttacaatat catgcatata gaccatttca tgaatctgcg aaaactcaat gtccattgta
     1561 tacctcacat ttttaccgtg actcgatgtt actgttcaat aatcaccttc catcaatact
     1621 aaaattaata cccctaatgt gccgataaca aatatagtca ttctacgtaa cgtctccata
     1681 aggtgataac cttgatcaaa tgaatttcga atataataaa ggaaatcttt tgagatgttt
     1741 atcacgaata aagctattta tgttaccaat atcatttcga gtgcgcacac tatctatctg
     1801 ttttttcgag atataaaaaa caacgtgtaa gttatgtaaa ttgccgatgc tactgagtaa
     1861 cattatggta aaaaaagtaa aaatgagcga tttttgatag aataaagcat gagaaaaggt
     1921 gatatttgac attatcagaa gctgcgaatt cggattttgc tctaatcaaa tgagagaaat
     1981 gtaaaatgaa gccacgaaat attaataata gcctaccact gcaaccatta gttcctgatc
     2041 aggagaacaa aaataagaaa aatgaagaga aatccgttaa tccagttaaa atcacaatgg
     2101 ggtctggttt aaattatatt gaacaagaat ctcttggagg aaaatatcta acacatgatt
     2161 tgtcaataaa gatagcggat atttctgaag agataattca gcaagcaata ttatctgcta
     2221 tgagcatata taaattttcg ataacagatg atttaatgag tatggctgta aatgaactca
     2281 taaaactgac caaaatagag aataatgtag acctgaataa attcactact atatgcacag
     2341 acgttctatc ccccctcgtc accagacata ataaagaaaa aaaacaaacg acattctacc
     2401 cttctcaaaa atcccctttt taattttcat tgaaaaaatg gcaattgata ctatgaatcc
     2461 aggtcataca cattaaggca tttatgtaat gtcgtaatta agataactaa taaggtgaat
     2521 attagtaaaa caactaatga tgttattagt ttgtagtgaa cagtactttt accaataatg
     2581 aaaaatatac cataagcaat gcaagcttaa aactaataat ttaaaaaaga taaatgtaaa
     2641 aatattaatg agtcaatcaa taataattta gcatcacgag acacatcaca gaggaatatt
     2701 atgagcacag aaacaattga aatattcaat aatagtgatg aatgggcaaa tcaactaaaa
     2761 cacgcattat cgaaaggaga aaatctggca ttactacatg gtttaactcc tgatatcctt
     2821 gatagaatat atgcatatgc attcgactac catgaaaagg gtaatataac ggacgcagaa
     2881 atttattata aatttttgtg catttatgcg ttcgaaaatc atgagtatct aaaagatttt
     2941 gcatcagtat gtcagccaaa aaagaaatat caacaagcat atgaccttta caaactaagt
     3001 tacaattact ccccgtatga tgactattca gttatttatc gtatgggtca atgtcagatt
     3061 ggggctaaaa atatcgataa cgcaatgcaa tgtttctatc acattattaa caattgtgag
     3121 gatgatagtg ttaagagtaa agcgcaggca tatattgaac tcttaaacga taattcagaa
     3181 gataatggct aagcattaca atcatttggt taaatttgct ggttatagtg cagttaacta
     3241 ctataaccag cacctcttat gttttcttaa atcagcatct taaa
//
LOCUS       DAZPBM010000286         3257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPBM010000286 DAZPBM010000000
VERSION     DAZPBM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.289"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHUZQ_004235"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHUZQ_004235"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1027764.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHUZQ_004236"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHUZQ_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1027765.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHUZQ_004237"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHUZQ_004237"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1027766.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHUZQ_004238"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHUZQ_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1027767.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          767 a          866 c          776 g          848 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atacttt
//
LOCUS       DAZPBM010000287         3234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPBM010000287 DAZPBM010000000
VERSION     DAZPBM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.290"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1973
                     /locus_tag="ACHUZQ_004239"
     CDS             <1..1973
                     /locus_tag="ACHUZQ_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1027768.1"
                     /translation="AESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTAS
                     TKATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSS
                     ATNSTSESLAATPKAVKAAYDLANGKYTAQDATTAQKGIIQLSSATNSTSETLAATPK
                     AVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGACRAFGGSVSTTTGNWTTAQFIEWL
                     DSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHLAGAVIEVMGIKSAMTIRITTPTT
                     SSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPTASEIGALPSGGTAVSSVNLASKG
                     RVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHLKGMTAVGEGELLIGWSGTSGAHA
                     PAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALMQGQTFD
                     KSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGT
                     KTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQHSQTGPRTNSGSQPTGMFPAGSTQ
                     VSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLSGTAASAGAHAHTVGIGAHTHSVA
                     IGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            1973..2489
                     /locus_tag="ACHUZQ_004240"
                     /pseudo
     CDS             1973..2489
                     /locus_tag="ACHUZQ_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          963 a          717 c          817 g          737 t
ORIGIN      
        1 gtgcagaatc ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat
       61 catcggcgtc tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg
      121 ccacgacggc atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga
      181 gcaaaagtac ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg
      241 atattgcatc cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc
      301 tcagtagtgc gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg
      361 ccgcgtatga cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag
      421 ggataatcca gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa
      481 aggcagtaaa agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg
      541 cggatatccc tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg
      601 ggtcagtaag cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt
      661 ctcagggagc atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata
      721 ataaaatcat tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag
      781 taatggggat aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg
      841 gcggaacaac caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct
      901 ggcgaaggga ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt
      961 caggtggaac agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga
     1021 cagacaatac gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc
     1081 caacagcgta tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt
     1141 tactcatcgg ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac
     1201 gggatacgac cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc
     1261 ctgcagagtt ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg
     1321 gttatgccct gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg
     1381 cttatccgtc aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca
     1441 gtggtcgtgc cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca
     1501 gcgcatccag tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat
     1561 ccacgaataa caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg
     1621 cccaccaaca tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt
     1681 ttccggcagg gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa
     1741 ccagtggcac ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac
     1801 tgtccggcac agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata
     1861 cgcactctgt tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg
     1921 cggaaaacac cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt
     1981 cagaatgagt gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga
     2041 atttattggt gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacatcgt
     2101 ggcatctcgt tgaagatcat cggggtaaaa cggtttatga cgtggcttcc ggcgacgcgt
     2161 tatttatttc tgaactcggc tcattaccgg aaaatgtcat ctggttatcc ccggaagggg
     2221 agtttcagaa gtggaacggc acagcctggg tgaaggatac ggaagcagaa aaactgttcc
     2281 ggatccggga ggcggaagaa acaaaaaaca gcctgatgca ggtagccagt gagcatatag
     2341 cgccgcttca ggatgctgtg gatctggaga tcgcaacgga gaaagaaacc ttgttgctgg
     2401 aagcctggaa aaagtatcgg gtgttgctga accgtgttga tacatcaact gcaccggata
     2461 ttgaatggcc agtagcacct atagggtaaa ttcgtaatga ttacctaaat acgttattct
     2521 tttgttaaaa aagtgatttt gctataggta gggagtatgt tcttggatta agaataaaaa
     2581 atcgccatga aagagcttca tggcgagtac ataaacgtac aaaatgtgat gaaagtagag
     2641 tggcgtaagc cactatatta agatatgaca atagcgaaga aaagtaaata acattttatg
     2701 gacgtttaat tgttaattat aaaaactaga gtgcccgata ttgtttatct tgctttaaaa
     2761 taatgtactc aaaccaaatt tttgagctaa aagctcaaat aatataaatg caataataat
     2821 gtttaataag acaattagct tgctttgtat tttcatttcg gaatactgtt aagtaacttt
     2881 gatgacccta acaatagatc ggaattttct tagagtcaat ggtcactgtt tttgcttgca
     2941 aagcaaatgt tttggtggtt ataagttgtc ttaatatagt cacgcatgca acttgttcca
     3001 tgcggagatt aaagattgga atataacgcg aagatggaat taaattacat ttgcaataat
     3061 caatttatta ttactcaata attaccgata ttattattgt aaattaatca ttagagtgca
     3121 aaaagaaatg taacggcaaa ataagaggac gatagctgag aaaaaagcgt gctttcccaa
     3181 tctggggcaa atcacgtctg aatgacacct gaaaacaggt ggtagcctca agaa
//
LOCUS       DAZPBM010000288         3219 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPBM010000288 DAZPBM010000000
VERSION     DAZPBM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.291"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..496
                     /locus_tag="ACHUZQ_004241"
     CDS             <1..496
                     /locus_tag="ACHUZQ_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1027769.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            526..3042
                     /locus_tag="ACHUZQ_004242"
     CDS             526..3042
                     /locus_tag="ACHUZQ_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1027770.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3053..>3219
                     /locus_tag="ACHUZQ_004243"
     CDS             3053..>3219
                     /locus_tag="ACHUZQ_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1027771.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          939 a          771 c          731 g          778 t
ORIGIN      
        1 aagccgtgcc ccatttatta taaccccacc gctatttcgt attgaagctg gcgatgactc
       61 atcactgcga attattaaaa cagctgataa cctgcctgaa aataaagagt cgctgttcta
      121 cattaatgtt cgtgccattc cagcaaagaa aaaatcagat gatgttaatg ctaacgagtt
      181 gacgctggta tttaaaacac ggatcaaaat gttttatcgc cccgcacacc tgaagggacg
      241 ggtaaacgat gcgtggacat cactggaatt taaacgtagt gaccattcac tcaatatata
      301 taacccaact gaatattacg tcgtatttgc cggactggca gtcgataaaa ccgatctcac
      361 aagcaaaatt gaatatatcg cgcccggaga acataaacag ttaccacttc ctgcatctgg
      421 cggaaagaac gtgaaatggg ctgcaatcaa tgattatggc ggcagttccg ggacagaaac
      481 tcgtccactg caataacaaa tataaaaaac acaggtcatc agggaatgcc acaacgacac
      541 caccagggac ataaacgcac accgaaacag ttggcgctca tcatcaaacg ttgtttgccg
      601 atggtgctca ctggcagcgg catgctttgc actaccgcta acgccgaaga gtattatttc
      661 gaccccatta tgctggaaac tacaaaaagt ggtatgcaaa caaccgatct gtcacgtttt
      721 tcaaaaaaat acgcacaact accaggaact tatcaggttg atatctggct gaataaaaag
      781 aaggtttcac agaaaaaaat tacatttacc gccaatgcag agcaacttct gcagccacag
      841 tttacggtag aacaactacg tgagctgggt attaaggtgg atgaaatccc ggcgctggct
      901 gaaaaagatg acgatagcgt gatcaactcg cttgaacaaa tcattcccgg tacagcagct
      961 gaatttgatt tcaatcatca gcgacttaat ttgagcattc cccaaattgc actgtaccgt
     1021 gatgcaagag gttacgtctc cccttctcgt tgggacgatg gtataccaac gctgtttacc
     1081 aactactcgt ttacaggttc tgataaccgt taccgccagg gcaatcgtag ccaacgacag
     1141 tacctgaata tgcaaaatgg tgctaatttt ggcccctggc gattacgcaa ctattccaca
     1201 tggacacgca acgatcagac atcaagctgg aataccatca gtagttattt acaacgtgat
     1261 atcaaggcat tgaagtctca gttgcttctg ggagaaagcg ccaccagcgg cagtattttt
     1321 tccagctaca cctttactgg cgttcaactc gcttccgacg ataatatgtt gccaaacagc
     1381 cagcgcggat ttgccccaac ggtacgcggt atcgcaaaca gtagtgcaat cgtgactatc
     1441 aggcaaaatg gttatgtgat ctatcaaagc aacgtgccag cgggtgcctt tgaaattaac
     1501 gatctctacc cctcttccaa cagcggcgat ttagaagtca cgattgaaga aagtgacggt
     1561 acgcaacgtc gctttatcca gccttattct tcattaccca tgatgcagcg acctgggcat
     1621 ctaaaatata gcgcgaccgc tggacgctat cgcgctgatg caaacagtga tagcaaagaa
     1681 cccgaatttg ctgaagccac ggcaatatat ggtttgaata atacttttac gctgtatggt
     1741 ggcctgctcg gttctgaaga ttattatgcg ctggggatcg gtatcggcgg cacacttggc
     1801 gcactgggcg cgttgtcgat ggatatcaac agagctgaca cccaattcga taaccagcac
     1861 tcttttcatg gctatcaatg gcgtacgcag tacatcaaag atatcccgga aaccaacacc
     1921 aatatcgctg tcagctacta tcgctatacc aacgatggct attttagttt tgatgaagcc
     1981 aatacccgta attggaacta taacagtcgc caaaaaagtg aaattcaatt caacatcagc
     2041 cagacaatat ttgatggggt aagtctgtat gcctccggtt cgcagcaaga ctattggggc
     2101 aataacgata aaaacaggaa tatctcagtt ggggtttccg gtcagcaatg gggaattggt
     2161 tacagcctga attatcaata cagtcgctac acggatcaaa ataatgaccg cgcactctct
     2221 ttgaatctca gtattccgtt agaacgctgg ttaccgcgta gccgggtttc ctatcagatg
     2281 accagccaga aagatcgccc aacccaacat gaaatgcgtc ttaatggctc actgctggat
     2341 gatggtcgcc tgagctatag cctggaacaa agtctggatg acgataacaa ccataacagt
     2401 agcctgaacg ccagttaccg ttcaccttat ggcaccttca gtgccggata cagctacggt
     2461 aatgacagca gccaatacaa ttacggcgtt accggcggcg tggttatcca tactcatggc
     2521 gtgacgctct cgcaatatct gggcaacgct tttgcgctta tcgatgctaa tggggcttct
     2581 ggcgtgagga tacaaaacta tccggggatt gctactgatc cctttggcta tgcagtggtt
     2641 ccttatctca cgacttacca ggaaaaccgt ctctcggtag atactacgca gctgcccgat
     2701 aacgtcgatc ttgagcaaac aacacagttt gtggtgccca acagaggtgc aatggtagcg
     2761 gcgcgtttca acgccaatat cggttatcgc gtacttgtta cagtcagcga tcgcaacggt
     2821 aaaccgttgc cctttggcgc tcttgccagc aacgatgaga cggggcaaca aagtatcgtc
     2881 gatgagggcg gcatactata tctctctggg atatcgagta aatcacaaag ctggactgta
     2941 cgctggggaa atcaggcaga tcaacaatgt cagtttgctt ttagtacacc ggattcagaa
     3001 ccaacaacct ctgtattaca aggcacggcg cagtgccatt aaggataaaa aaatgaaaag
     3061 agcgcctctt ataacaggac ttttgttgat atccacatcc tgcgcttatg cctcctcagg
     3121 agggtgtgga gctgacagca ctagcggtgc gacaaattac agcagtgtgg ttgatgatgt
     3181 tacggtgaac cagacagata acgtgacagg acgggagtt
//
LOCUS       DAZPBM010000289         3185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPBM010000289 DAZPBM010000000
VERSION     DAZPBM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.292"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUZQ_004244"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUZQ_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1027772.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUZQ_004245"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUZQ_004245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1027773.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHUZQ_004246"
     CDS             complement(1715..1984)
                     /locus_tag="ACHUZQ_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1027774.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUZQ_004247"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUZQ_004247"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1027775.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUZQ_004248"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUZQ_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1027776.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          772 c          769 g          812 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatc
//
LOCUS       DAZPBM010000290         3045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPBM010000290 DAZPBM010000000
VERSION     DAZPBM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.293"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHUZQ_004249"
     CDS             <1..172
                     /locus_tag="ACHUZQ_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027777.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="ACHUZQ_004250"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="ACHUZQ_004250"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR1027778.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="ACHUZQ_004251"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="ACHUZQ_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="ACHUZQ_004252"
     CDS             2190..>3045
                     /locus_tag="ACHUZQ_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR1027779.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          710 c          836 g          804 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAZPBM010000291         3005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPBM010000291 DAZPBM010000000
VERSION     DAZPBM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.294"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(10..165)
                     /locus_tag="ACHUZQ_004253"
     CDS             complement(10..165)
                     /locus_tag="ACHUZQ_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1027780.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="ACHUZQ_004254"
     CDS             complement(368..775)
                     /locus_tag="ACHUZQ_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1027781.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="ACHUZQ_004255"
     CDS             852..1079
                     /locus_tag="ACHUZQ_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1027782.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="ACHUZQ_004256"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="ACHUZQ_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1562..2361
                     /locus_tag="ACHUZQ_004257"
                     /pseudo
     CDS             1562..2361
                     /locus_tag="ACHUZQ_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2368..>3005
                     /locus_tag="ACHUZQ_004258"
     CDS             2368..>3005
                     /locus_tag="ACHUZQ_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1027783.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKT"
BASE COUNT          791 a          649 c          829 g          736 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag aggaagagga agatctaaat actgatctcc ccctcgccaa
     2041 aaacgagcgt ctaaaaaatt cgagccggag gctattgagc tgcccgattg gttgccggaa
     2101 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
     2161 cagggcgcta acggggcgat acgggaactg gaaaaattcc gtcagcaggg ttttacacct
     2221 gagcaggtga ttcgacacag catcgccaat gaataccagg gcctgttcgc gccgaaaggt
     2281 gttcggcctg agacgttgct ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc
     2341 ccgccaggct tcagggggta acggaccatg aaaaatattg cgacaggcgg cgttctggaa
     2401 cgcatccgcc gactgacccc gccacatgta accgccccat tcagaacggt agcggagtgg
     2461 cgcgagtggc aacttgctga aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc
     2521 cagttgcggg tggaaaaaat tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc
     2581 tcgtttgcga attaccaggt gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa
     2641 tccatcgccg atgaactgat gaccgggtgt acaaattttg cgttcagcgg aaaacttggt
     2701 accgggaaga atcacttagc ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca
     2761 gtgattgtgg ttaccgtggc tgatgttatg agcgccctgc acgccagcta tgacgacggg
     2821 cagtcaggcg aaaaattttt gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa
     2881 attggcattc agcgcgagac gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc
     2941 cggacagcgt cgatgcgcag cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa
     3001 acatt
//
LOCUS       DAZPBM010000292         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPBM010000292 DAZPBM010000000
VERSION     DAZPBM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.295"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUZQ_004259"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUZQ_004259"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1027784.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUZQ_004260"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUZQ_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR1027785.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHUZQ_004261"
     CDS             2805..>2992
                     /locus_tag="ACHUZQ_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR1027786.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZPBM010000293         2945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPBM010000293 DAZPBM010000000
VERSION     DAZPBM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.296"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHUZQ_004262"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHUZQ_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="ACHUZQ_004263"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="ACHUZQ_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR1027787.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="ACHUZQ_004264"
     CDS             2354..2758
                     /locus_tag="ACHUZQ_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR1027788.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="ACHUZQ_004265"
     CDS             2811..2921
                     /locus_tag="ACHUZQ_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027789.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
BASE COUNT          875 a          617 c          629 g          824 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttatt
//
LOCUS       DAZPBM010000294         2943 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPBM010000294 DAZPBM010000000
VERSION     DAZPBM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.297"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHUZQ_004266"
     CDS             complement(<1..458)
                     /locus_tag="ACHUZQ_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1027790.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
     gene            complement(497..784)
                     /locus_tag="ACHUZQ_004267"
     CDS             complement(497..784)
                     /locus_tag="ACHUZQ_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027791.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHUZQ_004268"
     CDS             complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHUZQ_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1027792.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1417..2082)
                     /locus_tag="ACHUZQ_004269"
     CDS             complement(1417..2082)
                     /locus_tag="ACHUZQ_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1027793.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2251..2607)
                     /locus_tag="ACHUZQ_004270"
     CDS             complement(2251..2607)
                     /locus_tag="ACHUZQ_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027794.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(2665..>2943)
                     /locus_tag="ACHUZQ_004271"
     CDS             complement(2665..>2943)
                     /locus_tag="ACHUZQ_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1027795.1"
                     /translation="ELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVD
                     PDLIWSLPDGEIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
BASE COUNT          731 a          771 c          648 g          793 t
ORIGIN      
        1 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
       61 ccggcctgaa tggctaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatca
      121 ctggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc ggcctactga ttcacacagg taatcaacga aaccggacac cagttttcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaaacccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cacacacgct taaagtctgc gtgtatccac
      361 tcacctattt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      481 gttacacagc aggagttcaa tcctgctcaa gattgtagat gagtttattc ttcagcaaaa
      541 gcagaaaacc agccttaaaa ccaatctctt tcaaaacccg taatgatgtg acaaattcgt
      601 cctcacgcaa aataaaaccg tccgtcagaa gtccattaca aaaataaaat aacacagcac
      661 cgctcttcct ttgttgagat tgcaaacatc taatgcggca atggctgaca atcgctccat
      721 tctcaacgcg cacagcatag aggccatttt cactaaaaat ttcacgcaat tcttcgattt
      781 tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca tccctcttct
      841 ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat caaattcacc
      901 ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg ggcgggttaa
      961 gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg atcagctccc
     1021 cgcccggtca ctcttacttc ctggattcgt agtctacgaa gacagcgacc tccgtctggc
     1081 cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     1141 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     1201 ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
     1261 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
     1321 tttggtgttc atgcctgaag cagatagcct caagcacccg cagtcattct acttacctcg
     1381 ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt ctttaccgta
     1441 gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta gattggtatc
     1501 gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc gcttgctgat
     1561 ttcaatcata cgggacgagc cgccctgctt gcgccagtta taatcccagt acaccatgcc
     1621 cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat ttttccagcg
     1681 tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt acagcgctgt
     1741 ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg gctcccattc
     1801 cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac
     1861 gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact ctttctgagg
     1921 atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc
     1981 catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt ttgccccgtt
     2041 tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt ttattggggt
     2101 cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga tgtgtgactg
     2161 ctccccgccc tttcgggtgg tctcctgatg atttgagggt gcagaaatcc ctccggttaa
     2221 ggattaaatt tttaacagtg ctaaatttaa ttattcagtt ctggattttg tcgccctgcg
     2281 tatccgcgct ttcgcgttac gctcaatctg tatcagcttt tctatatttc tccgcctttc
     2341 ctgttcctcc tggcgcaata gccttacatc atctgccagt ctggtttctc tttttgccac
     2401 agagagcatc cagtcaaacg gctccacaac cgcaccgcag attttacagc ggacctgacg
     2461 ctctttttcg tcaacccgta cagaggcgtg atggcaatat ggtctttccg atggctcata
     2521 aagaaaatta acctgattac gagggtcatc ctcttttacc ggaaataaaa cgatattgct
     2581 taactcatct tctggtttta tttccatgct cctctccttt gatgcgaatg ccagcggtaa
     2641 ttgaagcctg atagctaatt tcattcacag tatcgcctcc tgaaaattac cctgatagaa
     2701 agccagtaca cgctgcatag cttcgctctt ccggcactcg cgacagatta tattcaggcg
     2761 cctgtcgtag cggcgtattt ctccgtcagg taatgaccag ataaggtccg gatcaacaac
     2821 aaccggtttc ctcaccttta ctctcgagag ttttttgcgg gcgctttgcc agtccttgcg
     2881 agcctgttca gacgggaata agccgtagcc agaattatat acatcaccac ttgcgaccag
     2941 ttc
//
LOCUS       DAZPBM010000295         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPBM010000295 DAZPBM010000000
VERSION     DAZPBM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.298"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHUZQ_004272"
     CDS             37..264
                     /locus_tag="ACHUZQ_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1027796.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHUZQ_004273"
     CDS             complement(265..639)
                     /locus_tag="ACHUZQ_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR1027797.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUZQ_004274"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUZQ_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHUZQ_004275"
     CDS             complement(2099..2674)
                     /locus_tag="ACHUZQ_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1027798.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          695 c          689 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZPBM010000296         2935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPBM010000296 DAZPBM010000000
VERSION     DAZPBM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.299"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(110..1597)
                     /gene="yeeO"
                     /locus_tag="ACHUZQ_004276"
     CDS             complement(110..1597)
                     /gene="yeeO"
                     /locus_tag="ACHUZQ_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR1027799.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1758..1833
                     /locus_tag="ACHUZQ_004277"
     tRNA            1758..1833
                     /locus_tag="ACHUZQ_004277"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1791..1793,aa:Asn,seq:gtt)
     gene            complement(1871..2821)
                     /gene="cbl"
                     /locus_tag="ACHUZQ_004278"
     CDS             complement(1871..2821)
                     /gene="cbl"
                     /locus_tag="ACHUZQ_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1027800.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          861 a          783 c          620 g          671 t
ORIGIN      
        1 tacagaggaa tcgtgagaac gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc
       61 tgttttttaa atttgaaatc gtttgctgaa ataatctgca ttttgtcgtt tattccgaca
      121 caactggctt tttttcacac ttttgcggtt cggatcgagg gtatttccac agccaacgtc
      181 cagtaaccat tcgccagtaa aacagcacgg cccgcacagc ccagtcggca aacattccca
      241 tccagacacc aaccacaccc cagccaagca tgatgcccag cacataaccg acgacaaccc
      301 ggcaacccca catgctcaac atagaaaccc acatggcgta acgggcatcg cgagcacctt
      361 taaatccagc gggtagcacc catgaggcgg accaaatagg cataaataaa gcatttagcc
      421 aaatcagaat cacaacgaca tgtttaacct gtggatcctg ggtgtaaaac gatgccataa
      481 ccccggcaaa gggagccgtt agccaggcga tggccgttaa tccaagagtg gaaagccaga
      541 acacatgccg caactgaatc tctgcttgcg ctatctgccc tacccccaac cttcggcctg
      601 taatgatcgt agaagcagag ccgagcgcac ttccgggtaa gttgataaga gccgcaattg
      661 aaaacgcgat aaaatttccg gcaataacac tggtccccat cccggcaacg aacatttggg
      721 ttaataaccg accactggta aataacactg attcgacact cgcgggaata ccaatcccca
      781 tgacttccca gataatgcta aaattcagcg gtttaaaata gctctttaac gaaatcctta
      841 gcgcaggatt aaaaccaatc gccagcaccc acaaaattgc aactgcgcca atataacgag
      901 aaatggttaa acccagccct gccccgacaa atcccagtcc cggccaggag aaaaggccgt
      961 aaatcaatat gccgctaata ataatattaa gaatattcag gctaccgtta atcaatagcg
     1021 gtattttcgt attccctgca ccacgaagtg ccccgctacc aataagagtg atggcagctg
     1081 ctggataact gagtaccgtc agctccagat aagtcaacgc cagtgcttta acttctgtcg
     1141 tggcatcacc cgcgacgaaa tcaataattt gttcgccaaa atgatgaata agcgttgcca
     1201 acagtacggc aaacaacgtc atgatcacca atgactgccg cgtcgccacc ctcgctcgtc
     1261 gtcgatcccg cttaccgaga ctaaatgcca caacgacagt agtaccaaga tcgatagcag
     1321 caaaaaaagc cataatgacc atattgaagc tgtccgccaa tcccacgccg gccatcgcat
     1381 cttttcccag ccagctgacc agaaaagtgc tcagaacccc catcaacagg acacaggcat
     1441 tctccatgaa gataggaaca gcaagcgggg ttatctcgcg ccagaacaac actttgtagc
     1501 tcttgcgttt agcgtgccag cgagtgccgt gaacaacctg gcgtaaagca gaggagatat
     1561 tcaaagccga ccttaattgc agaaagtgaa accacatttc aaataatgag ggagaatcag
     1621 caaagctgca aagattttcg ccaacaaatt gtctgcaaat gcaacaaact gttgatagaa
     1681 acggcaaaca gttggggaat ttaaaaatcg ggtttgacaa aagatttttc gccgttaaga
     1741 tgtgcctcaa cagcgattcc tctgtagttc agtcggtaga acggcggact gttaatccgt
     1801 atgtcactgg ttcgagtcca gtcagaggag ccaaatttct gaaaagcccg cttttatagc
     1861 gggctttttg ctatatctga taatcaattt cctcttcact gttttccatc acctgccgct
     1921 tgatatcctc aactgacagt cctgcattac aaagttccag aaagcgccag acatagttac
     1981 gctgaagttg tcctcgcttc agtcccaacc agacagtatt agcatcgaaa agatgccgcg
     2041 tatccagacg gattaaattc tcttcctctt gttcgccgct ggattgttcg gcaactaatc
     2101 cgatcccaag cccaagagca acataggttt taatgacatc agaatcctgc gcacttaata
     2161 caatatctgc cagcaaacct ttgcgggcaa atgcgtcatc aatacgtgag cgccccgtaa
     2221 tcccctgtcg gtaagtgatt aacggccact tcgctattga ttccagcgtc aatggtgtaa
     2281 tttgcgttaa gggatgatcg agtggaacaa gcaaactatg gtgccaacga aaccacggga
     2341 aggcgacgag ctgcgggtca ttactcaaac gctcgctggc gataccaata tcagcttcgc
     2401 cattttgcaa caacgtcgca atttcctgtg gcgtcccctg gattagctcg agccgaacct
     2461 ccgggaaaag ttcgcgaaaa gctttaatga cctctggcaa gctataacgt gcctgagtat
     2521 gcgtcgttgc aatagtgaga acgccagacg tatcgttggt aaacaggtct gcaagccgac
     2581 gaacattact ggcttcattc agaatacgtt ctgcaatgac cagtaatgct ttgcccggtt
     2641 cagtcatgcc cagcagtcgc ttacctcgtc gaacaaatat ttcgatgcca agttcatcct
     2701 ccagttcccg aatatgacgg ctgacgccgg actgtgaggt aaaaagcata ttcgcaacct
     2761 ctgtcaggtt gtaatcctga cgtgcagcct cgcggattat ctttagttgt tggaaattca
     2821 cggtaaactc cgggcagttc agatttcccg ttattgttaa agtctaatgc ccggcataac
     2881 aaataataaa aacccgcatc ttattccatc ccgatataac gcttagctca ccaat
//
LOCUS       DAZPBM010000297         2917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPBM010000297 DAZPBM010000000
VERSION     DAZPBM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.300"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHUZQ_004279"
     CDS             <1..493
                     /locus_tag="ACHUZQ_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1027801.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHUZQ_004280"
     CDS             493..1083
                     /locus_tag="ACHUZQ_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR1027802.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHUZQ_004281"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHUZQ_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHUZQ_004282"
     CDS             2170..2658
                     /locus_tag="ACHUZQ_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1027803.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>2917
                     /locus_tag="ACHUZQ_004283"
     CDS             2682..>2917
                     /locus_tag="ACHUZQ_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1027804.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          831 g          692 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacga
//
LOCUS       DAZPBM010000298         2900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPBM010000298 DAZPBM010000000
VERSION     DAZPBM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.301"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHUZQ_004284"
     CDS             complement(<1..1199)
                     /locus_tag="ACHUZQ_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR1027805.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHUZQ_004285"
     tRNA            1492..1586
                     /locus_tag="ACHUZQ_004285"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2900
                     /locus_tag="ACHUZQ_004286"
                     /pseudo
     CDS             1858..2900
                     /locus_tag="ACHUZQ_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAZPBM010000299         2879 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPBM010000299 DAZPBM010000000
VERSION     DAZPBM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.302"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHUZQ_004287"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHUZQ_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1027806.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHUZQ_004288"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHUZQ_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1027807.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHUZQ_004289"
     CDS             1650..2099
                     /locus_tag="ACHUZQ_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1027808.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHUZQ_004290"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHUZQ_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1027809.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHUZQ_004291"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHUZQ_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1027810.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2879
                     /gene="yncL"
                     /locus_tag="ACHUZQ_004292"
     CDS             2787..>2879
                     /gene="yncL"
                     /locus_tag="ACHUZQ_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1027811.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          691 a          738 c          692 g          758 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttatt
//
LOCUS       DAZPBM010000300         2800 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPBM010000300 DAZPBM010000000
VERSION     DAZPBM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.303"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            8..511
                     /locus_tag="ACHUZQ_004293"
     CDS             8..511
                     /locus_tag="ACHUZQ_004293"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1027812.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="ACHUZQ_004294"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="ACHUZQ_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1027813.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="ACHUZQ_004295"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="ACHUZQ_004295"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1027814.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..>2800)
                     /locus_tag="ACHUZQ_004296"
     CDS             complement(2211..>2800)
                     /locus_tag="ACHUZQ_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1027815.1"
                     /translation="QSKDFSCDQSKIKIAETLFFNLDKEPYKNSRKLQELIWDKLVAY
                     VNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFLKDFFMKQPDFAKWYFYQL
                     LKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGYVVKIFEEKMGNELKIRTRMMKILR
                     NGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWNN"
BASE COUNT          754 a          650 c          617 g          779 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggt ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat
//
LOCUS       DAZPBM010000301         2797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPBM010000301 DAZPBM010000000
VERSION     DAZPBM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.304"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..617
                     /locus_tag="ACHUZQ_004297"
                     /pseudo
     CDS             1..617
                     /locus_tag="ACHUZQ_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
     gene            614..1093
                     /locus_tag="ACHUZQ_004298"
     CDS             614..1093
                     /locus_tag="ACHUZQ_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR1027816.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="ACHUZQ_004299"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="ACHUZQ_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1027817.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2249..2305)
                     /gene="ysgD"
                     /locus_tag="ACHUZQ_004300"
     CDS             complement(2249..2305)
                     /gene="ysgD"
                     /locus_tag="ACHUZQ_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1027818.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2467..>2797
                     /locus_tag="ACHUZQ_004301"
     CDS             2467..>2797
                     /locus_tag="ACHUZQ_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027819.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          843 a          619 c          591 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaaa attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttcccttg tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAZPBM010000302         2779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPBM010000302 DAZPBM010000000
VERSION     DAZPBM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.305"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            84..2363
                     /gene="ydeP"
                     /locus_tag="ACHUZQ_004302"
     CDS             84..2363
                     /gene="ydeP"
                     /locus_tag="ACHUZQ_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1027820.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2611..>2779
                     /gene="safA"
                     /locus_tag="ACHUZQ_004303"
     CDS             2611..>2779
                     /gene="safA"
                     /locus_tag="ACHUZQ_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1027821.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLT"
BASE COUNT          738 a          649 c          680 g          712 t
ORIGIN      
        1 atgttggcga taatcccttt gtatgtactg tgcatcatcg ctattacaaa tcctaataat
       61 tcatttccac acaggataag tagatgaaga aaaaaattga atcctaccag ggtgctgcag
      121 gtggttgggg tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc
      181 atgatgttat tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggttgtg
      241 catggccaga tcctaagcac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa
      301 tcgcctggga agtcacggat aagcaggtaa atgcctcttt ctttgctgag aatacggttc
      361 aatcattact tacctgggga gatcacgagc ttgaggctgc agggcgtctc actcagcctt
      421 tgaaatatga tgccgtcagc gactgttaca agccattaag ctggcaacaa gctttcgacg
      481 aaattggcgc acgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg
      541 gccgcacttc caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagca
      601 ataactttcc cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt ttggcagcga
      661 gtatcggtgt aggtaaaggg accgtgttgc tggaagactt tgagaagtgc gatttagtca
      721 tttgcattgg gcataaccct ggtacaaacc accctcgcat gctgacttcg ttgcgcgctt
      781 tagtgaaacg gggagcgaaa atgatcgcca tcaatcctct acaggaacgt ggcctggagc
      841 gatttaccgc accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttggcca
      901 gtgcctacta caacgtgcgc attggtggcg atatggcgtt gctcaagggg atgatgcgcc
      961 tgttaattga gcgcgatgat gctgcaagtg ccgcaggtcg gccctcattg cttgatgacg
     1021 aatttattca aacgcatacc gtcggctttg acgagctacg ccgtgacgtt ctcaattccg
     1081 agtggaaaga tatcgaacgt atttctggac taagtcagac acaaatcgcc gaactggctg
     1141 acgcatatgc cgctgccgat cgcaccatta tctgttacgg aatggggatc actcagcacg
     1201 aacatggtac ccagaatgta cagcaactgg tcaatctgct gttgatgaaa ggtaacattg
     1261 gcaagcctgg tgcgggtatc tgcccactac gaggtcactc taatgtacag ggcgaccgaa
     1321 ccgtcggtat caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg
     1381 gcttcacccc acctcatgca cctggacatg ctgcaattgc cagcatgcaa gcaatatgca
     1441 cggggcaggc tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc
     1501 gggaagcgag cgctgtaccg ttaacgcaat tagatttggc ggtacacgta gccactaagc
     1561 ttaaccgctc tcatctgctg accgcacggc atagctatat tctgccggtt ctggggcgta
     1621 gcgagattga catgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga
     1681 tgattcatgc ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg
     1741 cagtggtcgc gggaatcgcg caggcagcac taccccacag cgtggtagcc tgggagtatc
     1801 tggtggaaga ttatgatcgc attcgcaatg acattgaagc tgtgttacca gagttcgccg
     1861 actataacca gcgcatccga catcctggag gttttcacct gataaatgca gctgctgaaa
     1921 ggcgctggat gacgccgtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag
     1981 atccctcttc agcgtttaac agtaagctgg tcatggcgac agtacgtagc cacgatcagt
     2041 acaacacgac gatttatggt atggatgatc gctatcgagg ggtattcggt caacgagatg
     2101 tggtctttat gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta
     2161 atcttattgc gcttacgcca gacggtaagc gcagctcacg ccgcatggat agattaaaag
     2221 tggtcattta ccctatggct gaccgctcac tggtgaccta ttttccagaa tcgaatcaca
     2281 tgctaactct tgataaccac gatccattaa gtggcattcc tggttataaa agtattccgg
     2341 ttgaattaga accatcaaat taattcgcct cctcatttct tctgctgtca tccgcacagc
     2401 agaagaattt ctcattgact attatcgcgc aatttgctca cattgattaa attaaaccac
     2461 acactataag atataaacct ctgcctacag ctgtaagaaa ctccgctcag tactgaagtg
     2521 ccagtcctat ttcctctttt ctccagcctg ttatattaag catactgatt aacgattttt
     2581 aacgttatcc gctaaataaa catatttgaa atgtatgcga ccacagtgaa aaacaaaatc
     2641 acgcaaagag acaactataa agaaatcatg tctgtaattg tggttgtctt attactgacc
     2701 cttacgttga tagccatttt ttcggcaatt gatcagctgg gtatttcaga aatgggtcgc
     2761 attgcaagag atcttacac
//
LOCUS       DAZPBM010000303         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPBM010000303 DAZPBM010000000
VERSION     DAZPBM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.306"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHUZQ_004304"
     CDS             complement(423..680)
                     /locus_tag="ACHUZQ_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027822.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHUZQ_004305"
     CDS             complement(677..1174)
                     /locus_tag="ACHUZQ_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR1027823.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHUZQ_004306"
     CDS             complement(1376..1834)
                     /locus_tag="ACHUZQ_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1027824.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHUZQ_004307"
     CDS             complement(1831..2328)
                     /locus_tag="ACHUZQ_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1027825.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUZQ_004308"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUZQ_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR1027826.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          649 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaa
//
LOCUS       DAZPBM010000304         2704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPBM010000304 DAZPBM010000000
VERSION     DAZPBM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.307"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..248
                     /locus_tag="ACHUZQ_004309"
     CDS             <1..248
                     /locus_tag="ACHUZQ_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR1027827.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(288..1496)
                     /locus_tag="ACHUZQ_004310"
     CDS             complement(288..1496)
                     /locus_tag="ACHUZQ_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1027828.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1504..2005
                     /gene="tnpA"
                     /locus_tag="ACHUZQ_004311"
                     /pseudo
     CDS             1504..2005
                     /gene="tnpA"
                     /locus_tag="ACHUZQ_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2023..2691)
                     /locus_tag="ACHUZQ_004312"
                     /pseudo
     CDS             complement(2023..2691)
                     /locus_tag="ACHUZQ_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          625 a          665 c          666 g          748 t
ORIGIN      
        1 tggctgccct gcccgtaagt tggatccaga tgctggcagg tctggcgctg ttgagtacca
       61 ttggcggcag tttgtatcag gcgctgcata atgagcgtga gcgtgagcgc gacgcggcgg
      121 tggtggcatt tctggtaacg gcaagtggat tgacgctggt cgggattggt tctgcgttct
      181 ggggattaat tgccggaggc gtttgttacg tggtgttgaa tttaatcgct gacagaaacc
      241 gatattgaca tcctccacgc cctgaaggac gtggatccct gctacgttca ggctgtcgcc
      301 tgaatcattt cggtgggttc ctgcttcaac gggcggcctg actgcaccat ctctccacag
      361 gcaagaacgg cgtgccccgc cgctaaaatg ttacgagcac catttacatc ggcgttcgct
      421 gtatatccac atacctggca tctgaattta ctttgtgaca ggcggttctc tttcgctgta
      481 tgaccacagt acgcgcaacg ctggcttgta tacgctggcg gaacagcaag cacctgaccg
      541 ccacgccaga gctgcttgta ctcaagctgg cggtgcattt cataccagcc ctgatccagt
      601 atcgaacggt ttaaacctga ttttgcccgg acattgcgcc ccggctggct gaccgtaccc
      661 gctgctgact ttgacatgtt gctgaccttc aaatcctcaa tgactatcat tgcgtggttt
      721 ttgctgacgg ccgttgtgac tttgtgaagg tagtccctgc ggatatttgc gatacaggaa
      781 tgcagtcgct gtattttgcg tttctgcttc tgccagttgt tgctgaattt gaccttgcgg
      841 cttaactgtc gctgaagtct cgccagcgtc ttctggtttt tctgaaaact gtttacaggc
      901 tcaaagactg tgccatctga cagcgtggcg agtttagcca cgccagcatc cagcccgacc
      961 attgatgctg aagggtgagc cggagtggat acttcacttt ctgtctgaat actgatgtac
     1021 cacgtaccgc aggactggct gacagtgaca tttttcacaa cacccgtgac ctgacggcta
     1081 ttccggtagc gcatccagcc cagtttcggc agaaaaatac ggctgttttc ctgatcgagc
     1141 ttaacgccct gcgggtagcg gaatgcatca ttctgtcccc gctttttgaa tcggggaaaa
     1201 gcagcccgct tccggaagaa gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc
     1261 aatggctgtg agggagaatc tttaagccat tgcgtttcag tggcattttt ccactcaacc
     1321 agccaggaag ccattttccc gtaagggatg tatttattac cggcctcatg attctcattc
     1381 tgacgtgcca gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca
     1441 cactcctgtt gaccacctgg tcttaactgg aatttaaatg cctgtaatcg tttcacaggt
     1501 caaatgatac tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt
     1561 ttcctgatgc atgttcacct ggtctttgtc actagatacc gacgccagat tttttgacgc
     1621 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag ctgaactggt
     1681 tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactaacctc ccaaactggc
     1741 gatatccagt ctggtaaaca gcctcaaagg cgtatcgggt aggttactgc gacgagatcg
     1801 accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg gctattttgc
     1861 cagtagctgc ggaggtgtgc caatatccgt catccgccaa tacattgaac aacagcaaac
     1921 acctggtcag gtggaaaacc gcgccttata tccccgccct gaaggatggg gttttacggc
     1981 gcaccggata aaaagcgcca tctaacgatg gcgcgtctgg aactactttt tgttaacgtc
     2041 aaacatggtg gcatcggtcg ccatgtcatc aataacttgt ttaatatttt cgaaagccat
     2101 tggcgtactt tcgttattca ggtctttacc ttcgccctga cgaacgactt tgataaccgg
     2161 tttattagtc gctgcatcaa tcagctcacc ttcaaaatag aggcgagtat ccatggtacg
     2221 gtggcctgta gccatttgcg tccccgcaac cactaatgca acaggaacca cttcatagaa
     2281 ttgcagccct tctttgctgg tatctacacc ggtaatggcc ccacggaaaa tcagactacg
     2341 cggcccagcg gtggtaacca gtggtttacg ctgcgctatc gcttctttca tttcggtgtt
     2401 ggtatagttc aaaattttat ccagaacttt ctgccctacc taggtagaag gttttggcac
     2461 cggataataa gtgattgggt tccagacgat gctgtcatat ttgctttgat caaaactcgg
     2521 gtctacccaa cgtaaaacag gtttacctgt tgctgaggtt gtttctttta aatcagagta
     2581 attgtttaaa aaaccagaat atttatctgg ctgggtgatt ttagaggcac aaccagacag
     2641 agccaacaag ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca tgaatagttt
     2701 cctg
//
LOCUS       DAZPBM010000305         2588 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPBM010000305 DAZPBM010000000
VERSION     DAZPBM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.308"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            196..1149
                     /locus_tag="ACHUZQ_004313"
     CDS             196..1149
                     /locus_tag="ACHUZQ_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1027829.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHUZQ_004314"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHUZQ_004314"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1027830.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2481..2576)
                     /locus_tag="ACHUZQ_004315"
                     /pseudo
     CDS             complement(2481..2576)
                     /locus_tag="ACHUZQ_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          714 a          637 c          578 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatccccc
//
LOCUS       DAZPBM010000306         2561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPBM010000306 DAZPBM010000000
VERSION     DAZPBM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.309"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(1..803)
                     /locus_tag="ACHUZQ_004316"
                     /pseudo
     CDS             complement(1..803)
                     /locus_tag="ACHUZQ_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(856..1452)
                     /locus_tag="ACHUZQ_004317"
     CDS             complement(856..1452)
                     /locus_tag="ACHUZQ_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1027831.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHUZQ_004318"
     CDS             complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHUZQ_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR1027832.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2096..>2561)
                     /locus_tag="ACHUZQ_004319"
     CDS             complement(2096..>2561)
                     /locus_tag="ACHUZQ_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1027833.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          712 a          552 c          513 g          784 t
ORIGIN      
        1 aagcatagcg gtaaaacaag tgggattagt caacttgaca ttgagcagat cgaaatcgat
       61 tttgataaaa taactatgct catttatacc ttggcctgcg ccataggctt taaatgaata
      121 cagataattt gatgagctgg ataatttgat ctgttgagtt actgttggat caaagtttgt
      181 atctgtatag aattcaattg taagggtctg ataaatacca cctacagccc agtatttacg
      241 ggtgctattc gcattattgc aactccaata taacacgtct tcgcttggat tataccacga
      301 aaaatattgt tctgcagagt caccaatgta tatcccaggt gaactaacgt tagttacggt
      361 accgtaagct gaccacatgt ttgaaattaa taatgtgtaa tacaaaccag gaactgatgt
      421 attaaataat ttatgggagc catacatttt acctgagtca accattgcat tttcaatacg
      481 cattgcgcta ccacttgtgc tatcacttct gttgcaataa agaaaaccac caccagaagc
      541 ggtatctaac tgactccact gtaattttac acctgtaggt ccaagctgcg tcggtttgta
      601 gaaaactaaa ttatttgtac ttgttgcata tctgaatatg acagtagcat cgccattcgg
      661 ccccgtttct ctccattttt gatctgacgg accaacgccg taactattcc caacaatata
      721 tcctacgtta tcatcagtgc cagcactcgc ggtataacaa ctcaagcaca gaattaaaaa
      781 aaatgtctga agaatttctt cattatcact ccaccatcta ctgctgtaac agtcgcagtt
      841 atttgttttg atctattatt cgtagtacac atcaactaat atatgactca ttacttggcc
      901 agatatgaca tccagcgcgg gatcgatttt ttgcattctg gcataaaatt tccatgaaga
      961 attatgcagg gcagaagtca ttactggata cacggaacga gaatttcctg ctgaactaat
     1021 aacattgaat atattggtcg ggtcctgaat ggaaaagaga acgacaccga catttttagc
     1081 cccggttgcg acttcattgg caaatatttg ccttgaacct gcagccatga caccgctaag
     1141 ggctctaaaa tcaatatgta actgattaag cacatcgcca gttccctgta atgcacagtc
     1201 tgatactgtg acagtgaact cttttccacc tggttgatag ctttcaggag taacattatc
     1261 tgaaaaatat cccaccccaa cgacgccaag atcaatatta ccctggttac tgattccgct
     1321 tttgcaagtg ctgtttttta cattggcaac aaggtcaata tcctgcccag cgatggctgg
     1381 ataggtaatg ggaaactgta gtgacagaaa cagaattatt ctcttcatca gcttacgttg
     1441 agtgggatgc atatatagtt cctggatctg tcagtctgtt agttataggt tactgtaaat
     1501 gtggcaggtg cagaaaattt acccgcagtg acattgctta cggttttatt tttttcgacg
     1561 accagtcgag cactcatcgg ataataaaca accgcgttgg ctgcattgga gatagtgacc
     1621 tcttgagatg ctggtgtcac acaactaaat tgtgttgcgc tccctgttgc cggagtttcg
     1681 gtgctccaga cttcaacggc gacggcactt gctttatctg cggctgtgga tccatttgcg
     1741 agccccgcac cgtcattagc agtaccttcg catgtggctc gcttggttaa ttttatttgt
     1801 gcttttttat tggggatacc cgcacagtct ttgaatttga gtttgaatgt tttaactttg
     1861 gtcttggcat tgatttcaga aatatataca tcgccaaagt caacaacaga aatgtcctga
     1921 tcagagtcat ttactaatgt agcggtacaa gtacctattt ctacggtagt aatcacggtc
     1981 agcccaatgc tgtctgttgc cgcataagag ggtacgctga acaatgaggc agccaaaacg
     2041 acactgctgg ataatccata acgtaaattt ttaaatgtca tcttaattat ccttcttatt
     2101 gatagatgaa attaaacgtt gccagtgcac gaaaattccc cggtacgcct tgccctgcat
     2161 ctgtttcacg tagcgcaacg gtcatttcaa gacccttatc gggctgcatc tcgtctgtgc
     2221 tccagcgtat cttttccgca ctgttaggtt taaggaaagt ggcatcatca gtagtccgtt
     2281 ttttgaaacc cataccgata ttacttgtcg tcgaagatga atcaccagac tgcggtataa
     2341 taagcttagg tgagcttgat gatgcatttc cggtcagagt ggtatcaatc caactgatgc
     2401 cactgctgca cccactagca accagtttaa aatcagcctg agattctgtc gttttattcg
     2461 cgatcttatc cagacccatc ttagggattc tcaatgtgta gttattattg ccatcattcg
     2521 tgacgttatt accagtaagt gtgatgttac aggttgtcgc t
//
LOCUS       DAZPBM010000307         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPBM010000307 DAZPBM010000000
VERSION     DAZPBM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.310"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUZQ_004320"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUZQ_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1027834.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHUZQ_004321"
     CDS             complement(390..1178)
                     /locus_tag="ACHUZQ_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1027835.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUZQ_004322"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUZQ_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1027836.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUZQ_004323"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUZQ_004323"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1027837.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHUZQ_004324"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHUZQ_004324"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1027838.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          664 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZPBM010000308         2548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPBM010000308 DAZPBM010000000
VERSION     DAZPBM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.311"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..82
                     /locus_tag="ACHUZQ_004325"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHUZQ_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            829..1674
                     /locus_tag="ACHUZQ_004326"
     CDS             829..1674
                     /locus_tag="ACHUZQ_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1027839.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1685..1873
                     /gene="dicB"
                     /locus_tag="ACHUZQ_004327"
                     /gene_synonym="ftsT"
     CDS             1685..1873
                     /gene="dicB"
                     /locus_tag="ACHUZQ_004327"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR1027840.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1861..2061
                     /locus_tag="ACHUZQ_004328"
     CDS             1861..2061
                     /locus_tag="ACHUZQ_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR1027841.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2146..2421
                     /locus_tag="ACHUZQ_004329"
     CDS             2146..2421
                     /locus_tag="ACHUZQ_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027842.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          745 a          557 c          599 g          647 t
ORIGIN      
        1 agatgcaaaa cgcctccagc aactggagcc aaatgcaggc actggggcac gcatctggct
       61 ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct acaaagcaga
      121 aggccgtaca ggatattcac cgggtcttgg cggggtataa ataccatgcg cattgactga
      181 attcgcaaac aaaaacagac gcgcgatacc tggatagtcg gtctgcatag tcagatatct
      241 ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg tccgggagtt
      301 gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag agcatatatt
      361 tatgaagccc ttcgtaatcc tctggctcag cattatatag ttctactctg gcgagatacg
      421 ttggcatatt catttcctta ctggttgtgt gagaacttca gtaaagatac caccaaagcc
      481 cggaggtggt gaaataaaac cgggcacaac acgaaggcgc atttccggta tccataaaga
      541 gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc tccggttgta aataacgaca
      601 ttgctgtgtg aagtaccagt tggcggcatc ggtttaattg ctggctgatg tccgcccttt
      661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca gttaaaacag
      721 taaggaaaat cccttattcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
      781 aggcaccgca tcaacaaagt tcacttcggt gatgaaagga aagagaaaat gttgaatgta
      841 gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa aacgggtgcc
      901 gggattgcta ccccgaagtc attcacggcg cataaccgcg ctcaggcggt ttttttatgc
      961 gtaatgcaca gccacattca aattatggtg gggcgtgcgg ggcagtcgca agactggccg
     1021 ggttccgtga tgaccggttg tagcaaccct gtacgtctca ccacccatga gattgctacc
     1081 tccggtggtg agttaatcaa attcatcgcg gaggctgcca tcatggctac taccctttct
     1141 cacccttgtg taaccgtaga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
     1201 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatattga atgctcagat
     1261 aaatttactg ccctcaattt tgaagcgagc gaatacaccg actcaatcgg gcgcaaactc
     1321 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg ttatgggctt cactggcaaa
     1381 aaagccgctg cattcaaaga agcctacatt gctgagttcg accgcatgga ggccgaactg
     1441 cgccagaata acaccccacc tgctgacaag atgattccgg gtgatggtcg cactctggtt
     1501 gttcacttcg acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg
     1561 gtctgtaccc tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac
     1621 cggggcgcaa ttaaaaatat gactgtggaa caattactca aaattcattg ttgaggacgc
     1681 aataatggag acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt
     1741 cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac acgaacggga
     1801 gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg
     1861 atgtgcacaa tgaatacagt atttgcactc gttctgacgg tttttcttgt ttccggtgaa
     1921 ccggttgaca tggttactgg cgtatacagc tcaatgaaag aatgtatgac tgccgcagcg
     1981 aaacagaaaa ttcccggtga ctgttatccg gtcgataaag ttattcacca tgataataac
     2041 gaaatcccgg caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg
     2101 ccagcaatgg caggggttcg ctcaacctta attaaggaga aaaacatgat taccagttat
     2161 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
     2221 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
     2281 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
     2341 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
     2401 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgagtgaag
     2461 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
     2521 atgagcaccg ataaacaagt ttacccac
//
LOCUS       DAZPBM010000309         2531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPBM010000309 DAZPBM010000000
VERSION     DAZPBM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.312"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHUZQ_004330"
     CDS             <1..656
                     /locus_tag="ACHUZQ_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1027843.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHUZQ_004331"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHUZQ_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1027844.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHUZQ_004332"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHUZQ_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1027845.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHUZQ_004333"
     CDS             2258..2494
                     /locus_tag="ACHUZQ_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1027846.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT          656 a          559 c          645 g          671 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata a
//
LOCUS       DAZPBM010000310         2418 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPBM010000310 DAZPBM010000000
VERSION     DAZPBM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.313"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..274)
                     /locus_tag="ACHUZQ_004334"
     CDS             complement(<1..274)
                     /locus_tag="ACHUZQ_004334"
                     /EC_number="3.1.1.32"
                     /EC_number="3.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="catalyzes the hydrolysis of phosphatidylcholine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HFR1027847.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQL"
     gene            439..906
                     /gene="yigI"
                     /locus_tag="ACHUZQ_004335"
     CDS             439..906
                     /gene="yigI"
                     /locus_tag="ACHUZQ_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1027848.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            958..1845
                     /gene="rarD"
                     /locus_tag="ACHUZQ_004336"
     CDS             958..1845
                     /gene="rarD"
                     /locus_tag="ACHUZQ_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1027849.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1892..2191)
                     /locus_tag="ACHUZQ_004337"
     CDS             complement(1892..2191)
                     /locus_tag="ACHUZQ_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027850.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
BASE COUNT          658 a          546 c          534 g          680 t
ORIGIN      
        1 tcaactgaaa ctttacttca tccttacgcg cattttccgc ccagtcgtaa ctggcaatcg
       61 cttctttatt cagatcgctg gtttgggtgt aaatgaggta gttggtgtca taaggataga
      121 gcgtgaacgg attgtcatgc tcctgcagca tattggcgat aatactgcca cgcaccgctg
      181 gcgcgtcatg cacctctttc accgttgcct cttgtgcata tactgccata ggcaacataa
      241 acaccggcaa caaccagccc tgcagagtcc gcatcggtcg tgttctccgt aacgaattat
      301 tgataaggtg aatatttttc tgcgggctat tttacatact taactccata gccgttagtt
      361 atcccttcct aaagtagaaa ccaacaacaa agaagcataa aatcaacatt tcattaacca
      421 atgaagtgta aggatgatat gtctgccgta ctgaccgctg aacaagccct gaaattagtg
      481 ggtgagatgt ttgtttatca catgccattt aaccgcgcat tggggatgga actggagcgt
      541 tacgaaaaag agttcgcaca gctggccttt aaaaatcagc caatgatggt gggcaactgg
      601 gcgcaaagca ttttgcacgg cggggtcatt gcgtcggcgc tggatgtcgc cgccggtctg
      661 gtgtgcgtgg gaagtacctt aacccgccac gaaaccatca gtgaagatga actacgccag
      721 cggctatcgc ggatggggac cattgatctt cgcgttgatt atctgcgccc aggcaggggc
      781 gagcgtttta ctgctaccag tagcctgttg cgtgcaggca ataaagtcgc cgtcgcccgc
      841 gttgaattac acaatgaaga acagctttat attgccagtg ccaccgccac ctatatggta
      901 ggttgaagac ataaaagcag gtaaactgct gttaccaatt aaaaatgagt attcccgatg
      961 gatgcaaaac aaacgcggca gggcgtatta ctcgctcttg ccgcttattt tatttggggt
     1021 atagcgccag cgtacttcaa gttgatttac tacgtgcccg ccgatgaaat cctgacgcat
     1081 cgcgtgatct ggtcgttttt ctttatggtg gtgctgatga gcatttgccg ccagtggtcc
     1141 tatttaaaaa cgctgattca gacgccacag aaaattttta tgctggcagt ctctgccgtg
     1201 ctgattggtg gcaactggct actgtttatc tgggcggtga acaatcacca tatgctggaa
     1261 gcgagccttg gttactttat taacccgctg gtgaacattg tgctggggat gattttcctc
     1321 ggcgagcgat tccgccggat gcaatggctg gcggtgattc tggcgatatg tggcgtgtta
     1381 gtccagctgt ggacttttgg ttcgctacct attatcgcgc tgggactggc atttagtttt
     1441 gccttctacg gtctggtacg caagaagatt gccgttgaag cgcaaaccgg catgttaatc
     1501 gaaaccatgt ggctgctgcc cgtggcggca atttacctgt ttgctattgc cgacagctca
     1561 accagccata tggggcaaaa cccgatgtcg ctgaatttac tgctgatcgc cgccggtatt
     1621 gtcactaccg taccgctgtt gtgttttacc gccgctgcca cgcgcttgcg tctctcaacg
     1681 ttaggctttt tccagtacat tggcccgacg ctgatgttcc tgctggctgt gacgttttat
     1741 ggtgaaaaac cgggtgccga taagatggtg actttcgcct ttatttgggt ggcgctggca
     1801 atttttgtga tggatgcgat ttatacgcag cgtagaaaaa gttaagaatt aacatgggta
     1861 ggccgtttaa agagcctacc cactgactta tttatctttt tttgaaaaaa attaagataa
     1921 gaaaaagaac aatatttgtt acaggaaata cccccaaaac tccccagtac ttactccatc
     1981 ctagagacat cgtatatcta ccaaggattt gacaaaaccc aaaacttaac cctataccta
     2041 tacttcttaa atttaatgtc gtagtcaggc ttaaaataaa tagaacaagg atggtaatag
     2101 cccaccacaa taaccatgcc ttaacaaaat cagatttttt gctgtaaggt tctttaccca
     2161 agaacaactg ccaaaagtta ccatttccca tatagaatcc attcacccaa gatattgttt
     2221 atagaataaa aatacatttc aattatattt aattaaaata taccccatca ataaaaactt
     2281 acacactcta aattactact gtatagttat caatgcaaag actgctatat tgcatatata
     2341 tatgcaaacc acataattca ggtaatatat attcaaaaca attatatata taaaacaatg
     2401 taaaaatgtt caaatcat
//
LOCUS       DAZPBM010000311         2369 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPBM010000311 DAZPBM010000000
VERSION     DAZPBM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.314"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            128..889
                     /gene="adiY"
                     /locus_tag="ACHUZQ_004338"
     CDS             128..889
                     /gene="adiY"
                     /locus_tag="ACHUZQ_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1027851.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1026..2363
                     /gene="adiC"
                     /locus_tag="ACHUZQ_004339"
     CDS             1026..2363
                     /gene="adiC"
                     /locus_tag="ACHUZQ_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1027852.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
BASE COUNT          554 a          571 c          566 g          678 t
ORIGIN      
        1 ttgtaatatt agtaaaactc gttattttta tgcatgttta tattcatcat acaattatat
       61 aaccatttcc cggtatcgct ttgctttagc gagaactggt cttttattcg cactcaggag
      121 tacatgtatg aggatttgca gcgaccaacc ttgtattgtt ttattgactg aaaaagatgt
      181 ctggataagg gtgaatggga aagaacctat tagccttaaa gctaaccata tggcgttatt
      241 aaattgtgaa aataatatta tcgacgtctc ctctcttaac aacactttgg ttgctcatat
      301 tagtcacgac atcatcaaag attacctccg gtttctgaat aaagatctct cgcaaatacc
      361 agtatggcaa cgtagcgcta cgcccatact caccctgcca tgcctgacgc cagacgtctt
      421 tcgcgttgcc gcgcaacaca gcatgatgcc cgcagaaact gagtcagaaa aggaacgaac
      481 acgcgcatta ttattcacgg tgctatcccg ttttctcgac agtaaaaaat tcctttcatt
      541 aatgatgtat atgttacgta attgtgtaag tgacagcgtt tatcaaatta ttgaaagcga
      601 tattcacaaa gactggaatc ttagtatggt agccagttgt ttatgtctta gcccaagtct
      661 gttaaagaaa aagctgaaaa gcgaaaacac cagttatagc caaataatca ccacctgccg
      721 catgcgttat gccgtaaatg aattaatgat ggacggtaaa aatatctctc aggtatcaca
      781 gtcctgcggc tacaacagta cgtcgtactt tatttctgtc tttaaagact tctacggtat
      841 gacgccgctg cattatgtta gtcagcacag agaacgaact gtcgcctgat ttttaacctt
      901 aacgaagagc tatattaata acggcatcag cgataacccg gtcgataata attcaactat
      961 cgaatgcagg cgtatgatat gacgtaattt attgtcacga agctcgcctt cgcaggagtt
     1021 taattatgtc ttcggatgct gatgctcaca aagtgggctt aatccccgtc accctgatgg
     1081 tgtcggggaa tattatgggg tcaggtgttt ttctgttacc tgcaaacctg gcctccactg
     1141 gcgggattgc catttatgga tggttggtga cgattatcgg tgcgctgggg ctctcgatgg
     1201 tatacgccaa aatgtcgttc ctcgatccaa gtcctggtgg ttcttacgct tacgcccgcc
     1261 gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct ctactggctg gcctgctgga
     1321 tcggcaatat cgctatggtg gtcattggcg taggatattt aagttacttc ttcccgattc
     1381 tgaaagatcc attggtatta accatcacct gcgtcgtggt gctgtggatc ttcgtcctgc
     1441 tgaacattgt cggtccgaaa atgatcaccc gtgtgcaggc cgttgccacc gtgctggcgc
     1501 tgatccccat cgtcgggatt gccgtatttg gctggttctg gttccgtggt gaaacctata
     1561 tggcggcatg gaacgtcagc ggcctgggca ccttcggtgc gattcaaagt acccttaacg
     1621 ttacgctgtg gtcgttcatc ggtgtggaaa gtgcctccgt tgccgcaggt gtggtgaaaa
     1681 acccgaaacg caatgtccct atcgccacca ttggtggggt attgattgcc gccgtttgct
     1741 atgtgctctc caccaccgcg attatgggga tgatccctaa tgccgcactg cgcgtttctg
     1801 cctcgccatt cggtgatgcc gcacggatgg cgttgggtga caccgctggg gccattgttt
     1861 ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg ctggacgttg ctggcgggtc
     1921 aaacggcgaa agccgctgcc gatgacggac tgttcccacc gatttttgcc cgtgtaaata
     1981 aagcgggtac gccagtggcg gggctgatta tcgtcggtat tttgatgacc atcttccagc
     2041 tcagtagcat ttcgccaaac gcaaccaaag agttcggtct ggtatcttcc gtgtcggtca
     2101 tctttacact ggtgccatat ctttacacct gtgcggcgtt actgctgctc ggccacggtc
     2161 actttggtaa agcacgcccg gcatatctgg ctgttactac cattgccttc ctctactgca
     2221 tctgggccgt ggtggggtcc ggggcgaaag aggttatgtg gtcatttgtc accctgatgg
     2281 tcatcaccgc catgtatgcg ctgaattaca accggctaca taaaaacccg tatcccttag
     2341 atgcaccaat aagcaaagat taattcccc
//
LOCUS       DAZPBM010000312         2331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPBM010000312 DAZPBM010000000
VERSION     DAZPBM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.315"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHUZQ_004340"
     CDS             complement(25..315)
                     /locus_tag="ACHUZQ_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1027853.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHUZQ_004341"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHUZQ_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1027854.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHUZQ_004342"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHUZQ_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1027855.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHUZQ_004343"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHUZQ_004343"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2331)
                     /locus_tag="ACHUZQ_004344"
     CDS             complement(2171..>2331)
                     /locus_tag="ACHUZQ_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1027856.1"
                     /translation="VKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLG
                     TIRAFDVT"
BASE COUNT          643 a          621 c          516 g          551 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc t
//
LOCUS       DAZPBM010000313         2288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPBM010000313 DAZPBM010000000
VERSION     DAZPBM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.316"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..396
                     /locus_tag="ACHUZQ_004345"
     CDS             <1..396
                     /locus_tag="ACHUZQ_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027857.1"
                     /translation="EEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQRVKDARKLKPTSK
                     ENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAILILHQMLKPECLNL
                     AMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            406..906
                     /locus_tag="ACHUZQ_004346"
     CDS             406..906
                     /locus_tag="ACHUZQ_004346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027858.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            982..1293
                     /locus_tag="ACHUZQ_004347"
     CDS             982..1293
                     /locus_tag="ACHUZQ_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027859.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            1290..1583
                     /locus_tag="ACHUZQ_004348"
     CDS             1290..1583
                     /locus_tag="ACHUZQ_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027860.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            1644..>2288
                     /locus_tag="ACHUZQ_004349"
     CDS             1644..>2288
                     /locus_tag="ACHUZQ_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1027861.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHINLLIFGDQFDSILRDF
                     PASYPLNDKLLHGHFQSFIDSYKNIDFIISGHLPISDNRNIQVFGGANHNLLCHF"
BASE COUNT          786 a          330 c          480 g          692 t
ORIGIN      
        1 gaggaaatgt tatccggtga atatgctggt ttcgtttata tctttcagtt tgaggatggt
       61 tctaattatg tcggtgctaa gcaaatctat cagcgggtta aggatgcgag aaagctaaag
      121 cccacttcaa aggaaaatgg ctggcgtgag tacctcagta gttccaatgt cgttaaaaac
      181 aaaattgaaa atggtgaaca gtactcaaaa acgattctat ggtgcttcgc aactatgcga
      241 gaggtaatga ttgtagaggc aatactgatc cttcatcaga tgcttaaacc ggaatgtttg
      301 aatctggcaa tgatgagtaa gattagatca ccaaatgcaa aggataaaaa aagactactc
      361 ggaatagtac aggaactatt ggagtacttg aattgaggta aatatatggc aagtggaata
      421 aatgggattg gtagtataag gaactatata caaacgcagg gagttagatt aaataatcaa
      481 ttccagaatg aaataattaa acgttccaag ctactatccc aaagaatgca gggtgatttg
      541 aataatagcg ttgaccgtgg ggcagtgggc tttactcaac gttccatcct tttcttctat
      601 agaaaggttg ggagaaattc agttacatcg accatcatgg ttaaagatat tcaggctaag
      661 tacctttatc aagtactggt acagccaaag gcaattgata agtttatacc tacttcatct
      721 acaagattaa ctaagcaggg caacatttca ggactgaaaa agaatctttt aagtgaacga
      781 tataaggtag tgaagggtaa gaatggtaaa gaacgattaa ttgataccgc aaagaaagat
      841 accaagaaga aaaccaaacg tgtaattggc ttacgtgaga agaaacaacg tcgccttatc
      901 tattgacttc tataaggaag ccaatgatgg tgtaagggta attatgagcg gtattcaggg
      961 aacattcagg ataagtagac aatgaatttt gagcaacatt atggtgattt aataagcaag
     1021 attaagctaa atggcatgac accagatggt aatagtatgc caattaacaa atgttttctt
     1081 gataagaaat ttcaaaaggt cgttaagaaa agtaattatc agattgatag ctatatgaac
     1141 gttatttatg agaagaagaa agatttcaaa gttggtgaag tacttgaatg ggaattacag
     1201 ggtgaaaaga tgcctgtatt tctaattgag agtaaaaagc tctttgtgaa gggtaaacat
     1261 ttttgggtat atgccgtggg gattattgaa tgattagtat attgattgag tttctgaagt
     1321 caggattgga ttttttcatt aagaagaaaa ctattgaaca ggaagtaaag aaaacgaatg
     1381 tagaagggca gattgaaacg aataaagaag agattgaaaa ggtaacattc cactggcgta
     1441 atgccctcgg ttttgtacta acccttatca ttctctataa ctggatcata gtaccagtac
     1501 ttgatgcttt tggaattatt gtgatccaag ttccattagg acaacttttg caagtgctat
     1561 tgattatggt tggtggaagc tgaaagattt ttcctttaat atgtgttttg agttaataaa
     1621 atagcaatct aaggatttga tttatgagtg atgcaattac tacagcagca ataaaaaaat
     1681 tgtatggact atccgctggg caatgtaata tttgccgaac ttctcttttt aaagagcagg
     1741 ttcacatcgg acaaatggct catgtaatag ccaaaagccc aaaaggacca aggggcgatg
     1801 acctgttagc aaatgacaat tcttatgaaa atcttatcct gctttgtgca aatgatcata
     1861 ttcttgttga tagagatcca ttcacttata cagtagaaaa attgcatcaa atcaaaagtg
     1921 aacatgaggc ttatatacag ggtgcaaccg ataatacatt attttcagat aaaaaaagac
     1981 agtcagatgt ggacttttta aacgcttact tccactttac accgtttgcg agggtacgtt
     2041 cattagttga aactttacct aattcttttc acatcaatct tcttattttt ggagatcagt
     2101 ttgatagtat tttaagggat tttccagcga gctatccttt aaatgataaa ttactccacg
     2161 gtcatttcca aagttttatt gattcgtata aaaatattga ttttatcata tctggacatt
     2221 tgccgatttc cgataataga aacattcagg tgtttggtgg tgcgaatcat aatcttcttt
     2281 gtcatttc
//
LOCUS       DAZPBM010000314         2246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPBM010000314 DAZPBM010000000
VERSION     DAZPBM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.317"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(371..505)
                     /locus_tag="ACHUZQ_004350"
                     /pseudo
     CDS             complement(371..505)
                     /locus_tag="ACHUZQ_004350"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(518..1411)
                     /locus_tag="ACHUZQ_004351"
     CDS             complement(518..1411)
                     /locus_tag="ACHUZQ_004351"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1027862.1"
                     /translation="MSHIRPDFPEPYGLPVDGLRGKTSIFSHPVYLFLREFSLQDSRG
                     GSLSKKAESLSSGKITIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNEGILLNGKTIRKLMKELGISSLIRR
                     KKYRAYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
     gene            1378..2246
                     /locus_tag="ACHUZQ_004352"
                     /pseudo
     CDS             1378..2246
                     /locus_tag="ACHUZQ_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1608..1724
                     /locus_tag="ACHUZQ_004352"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          570 a          502 c          540 g          634 t
ORIGIN      
        1 gcccgaggta tattctgaaa tatcattcat catgaccggc gtttatacaa acgccggtca
       61 ttcgctaaca gcttcaccgt tccttccccc atccaggttc cgcatgagcg tgttgtaatg
      121 ttcataagcg tattatcagg attgtctgga aaattcccga tactttggac tattactcct
      181 gaacaattcg aaattcagca gattaaaggt aagcgttaac ggaggtaagc gttaacggag
      241 gtaagcgtta acggaggtaa gcgttaacgg aggtaagcgt aaaccgactg ccgtatgtag
      301 ccattaagcc agtattggta acgtaggtgc ccaccttttc aaatgatctg cgccccaaat
      361 cttggacatc ctatgaaaaa tgtttaagtc gatattgaac cggactcatg ccatcgagtt
      421 tctctttaat gcgatggtga ttgtagtatt caatgtaatc aattatatct tgctttagat
      481 gatgtaactt attgtattta tttaggtagt aacattctta ttttaaagta ctaaaaaagc
      541 tttccattgc tgcattatct aagcagttac cacgtcggga catgctttgt gttatgccat
      601 tatcactcaa ccaacgttgc caacgaggca tctgatattg ccacccctga tcactatgca
      661 acaagggctt atcgtcttta ttgagttgtt ctaccgcatc taatagcatg ctctttacca
      721 ttgatgctaa tgggcgctca gtaaggttat atgagactat ttctccatta tacaggtcca
      781 tgattggtga taaatagagc ttcctgccat cgaccttaaa ctctgtgaca tctgttaccc
      841 acttttgatt tgggcggtca gcataaaatt gtctcttcag taaattatta caagttcgcc
      901 cctgttctcc acgataggca cggtacttct tgcgcctgat aagtgagctg atacccagtt
      961 ccttcattaa ctttctgatg gttttaccat taagtaaaat gccctcgtta cggcaggcaa
     1021 gtctgatgcg tcgataacca taacgccctt tatgctgatg gtatattctc aatattactt
     1081 gtttaacaag ttgatatgga tctggcgcat taaggcgttt cacgtgatag taaaatgtac
     1141 tacgtggcaa atccgcgata accagtaatt gtgataatgg atgacattgc cttaactcat
     1201 taataatgat tactttttct tttgttccaa tcgtaatttt tcctgagcta aggctttcag
     1261 cttttttaga taatgaaccg ccccgggaat cctggagact aaactccctg agaaagaggt
     1321 aaacaggatg actaaaaata ctcgttttcc cccgaagtcc gtcaacgggc agtccgtatg
     1381 gttctggaaa gtcagggcga atatgactca caatgggcgg taatttgttc cattactcca
     1441 aagattggct gtacaccgga gactctgcgt gtctgggttc gccagcatga gcgggatacc
     1501 aggggcggtg atggagggct caccaccgct gaacgtcagc gtctgaaaga gctggagcgt
     1561 gagaatcgtg aactgcgctg cagtaacgat atccttcgcc aggcttccgc ttattttgcg
     1621 aaggcggagt tcgaccgcct ctggaaaaaa taatgccact gctggataag ctgcgtgggc
     1681 agtacggggt cggaccggta tgcagtgaac tgcatattgc cccgtcaacg tattaccact
     1741 gttagcaaca ggacatcatc ctgataaacg cagtgcccgt gcgcagcacg atgactggct
     1801 gaagagagag atacagcgcg tatacgatga aaatcatcag gtgtacggtg tgcgtaaagt
     1861 ctggcgccag ttgttacggg aaggaatcag ggtggccaga tgtacagtgg cgcgcctcat
     1921 ggcggttatg ggacttgtcg gtgttctccg gggtaaaaag gtccgcacta ccgtcagccg
     1981 gaaaaccgtt gccgcaggtg accgcgtaaa ccgtcagttc gtggcagaac gtcctgacca
     2041 gctgtgggtg gctgatttta cttacgtcag cacatggcag ggcttcgtct atgtggcgtt
     2101 catcattgat gtgtttgccg gatacatcgt gtggtgacga gtctcatcgt ctatggaaac
     2161 gacattcgtg ctggatgcac tggagcaggc gttgtgggcc cgtcgtccgt ctggcaccat
     2221 ccattacagc gataaaggct ctcagt
//
LOCUS       DAZPBM010000315         2218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPBM010000315 DAZPBM010000000
VERSION     DAZPBM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.318"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..302
                     /locus_tag="ACHUZQ_004353"
     CDS             <1..302
                     /locus_tag="ACHUZQ_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027863.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            391..>2218
                     /locus_tag="ACHUZQ_004354"
     CDS             391..>2218
                     /locus_tag="ACHUZQ_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR1027864.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQ"
BASE COUNT          702 a          394 c          423 g          699 t
ORIGIN      
        1 cacgtgtaga actgtattac cgctttaacc atgaagaaga atattaccct tctcaccgtt
       61 ttatgcttgt tgttgatatg aagacagtaa tggttttttc acaacgagct gaatcactgg
      121 ggttatactc gaataatcgc ttgcttatca atataattgc cgaacatatc catagtgata
      181 tatatctaac caagtatcag caacaatatc cagatgccaa tgcattaata aatactttgc
      241 atgaatctgg caatgatatg gtgcaggatg aaaaacattt gagtgatagc aaaaaccgtt
      301 aatattttcc tttcattgtg atcgtctgta ggcgatcaca atctatttgt cttttataac
      361 gtctaaactt ttttatgcaa aggtgtattt atgatttttt ttaacgatat aaagcccact
      421 ggttggttaa agaataagat ggaatggtat atgaacggtc atataggaga actggataaa
      481 ttagttcccc atcttattac ggagcataaa atatatggaa gagatcgtat aactccacgc
      541 actgtatctg ctgaagatgg cgttattaaa aaacagaata atcccgatga taacatgatg
      601 caatattatt ggtgggatag tgaaactcaa tcgaattgga aggatggata ttgccgcagt
      661 gcctacttgt tagataataa agaatggcag gaaaaagcga gcgatttatg tttggaactt
      721 atcgatacgc aagatgatga tggctacatt ggaatttatg attcatcatt aaaatttaac
      781 tgtaaagcag aaaacggaga gttctgggca caagctactg ccttgcgttt tgtgcttgga
      841 tgttatgagt ccacccgtaa tgagagttta ttaacttctc ttacccaagc cgcacaatgc
      901 attatggctg gctaccctaa agaaacgtca catcctttcg ttgtggaaca gggttttgct
      961 ggacatagcc acggattaac aataacagat gttttctacc gcctttatga aataaccaat
     1021 aacattgaat atctctcata ttgcttgtgg ctgtatgaag attattccgc aggatgcgtg
     1081 tcggagcaag atctgcaata taaaaatgtc atagaccctg gttaccttct taaaggtcat
     1141 ggcgtacata cgtatgagca tattagggca ttagccatcg cagcgagcca acggccgcag
     1201 tatcaaactg cattagatat ttttttatcc cagttgaaat attatcttac accatctggc
     1261 gctcccattg gtgatgagtg gattacagga cgcacagcta atgccactta tacaggctat
     1321 gaatattgtt ctgttcatga actatttagc acttatgtta tgttaataaa actgaccact
     1381 aatattggtc tggccgatga tgctgagtgg ctattctata atgctagttt tggcatgcag
     1441 catcccatac ttcacgcgat aatgtactgt aagactgata attgttatga agcaaatgaa
     1501 aggaaacatc ctgacagtaa tcagtttaat accagatata aatactcccc tactcatgag
     1561 gaggctgctg tctgctgcgt accgaataca gcaagaacca taccggcttt tgtcgaaaat
     1621 atgtttcaag aaaataacaa tggttttact gcattatttt atggcccgtg tgagttttat
     1681 ggcacattta ataatgttgc aattaaaatt acacaattaa ctgaataccc tcatgattta
     1741 agcgtaaaat gtctcattga gcctgagtca tctgttcgtt ttgctctcag tttccgttac
     1801 cccggctggg caaaaatgat gattattaat ggtgttactt ttactaaaaa tgatactcaa
     1861 aactcactta ttattttaga tagaacatgg caacatcatg atgtgattga cattaaattt
     1921 atagctgata tacagtttaa tactgactta tgtggtgaca cttatatttc cagaggtccc
     1981 gttttatatg caattgagct tgagagcgat attttaatca aaaaaaacct gctgaacggg
     2041 aaatattttg actcaggata cataccctgt agtagagctg atgaatctat ccagttctct
     2101 tatgctgata tggaatcttt ttcatattca acctcaccag agggcaaaca atacattgaa
     2161 ggctgttttt atttaaacaa gactaaaata gtcagaaaac ttataccatt tggtcaaa
//
LOCUS       DAZPBM010000316         2177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPBM010000316 DAZPBM010000000
VERSION     DAZPBM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.319"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            3..956
                     /locus_tag="ACHUZQ_004355"
     CDS             3..956
                     /locus_tag="ACHUZQ_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1027865.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            1165..2130
                     /gene="lipA"
                     /locus_tag="ACHUZQ_004356"
     CDS             1165..2130
                     /gene="lipA"
                     /locus_tag="ACHUZQ_004356"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1027866.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT          577 a          545 c          499 g          556 t
ORIGIN      
        1 gcgtggatag taataatcaa attgaaccct gtttaagcag aaagtcgtca gaaggtaaac
       61 cacaaatatt tacaacctta cgaaatattg atcttaacct tctgactatt tttgaagctg
      121 tatatgtaca taaagggatc gttaatgcag cgaaagtgct taatctgacc ccctcggcaa
      181 tcagtcagtc tattcagaaa ctgcgcgtta tattccctga cccattgttt attcgcaaag
      241 gccagggtgt cactcctacc gcatttgcga tgcatctaca tgagtatatc agtcagggcc
      301 ttgagtccat tcttggcgcg ctggatatcg aaggaagcta tgataagcaa cgaacgataa
      361 ctattgcgac cactccctcg gtcggagccc tggtccttcc tgtcatctat cgggcgatta
      421 aaactcacta tccgcagctt ttactgcgca acccgcccat cagcgacgcg gaaaaccaac
      481 tcagtcagtt tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat cgtacggtgc
      541 aacatcatgt tctgttcacc gacaatatgg tgttaatttg ccgcgaggga aatccactac
      601 tctctttaga agatgacaga gagactatcg acaacgctgc gcatgtactc ctgttaccgg
      661 aagggcaaaa tttcagcggt ctgcggcaga gagttcaaga gatgtttccg gaccggcaaa
      721 tcaatttcac cagctacaac attttgacaa tcgctgcact ggttgccaac agtgacatgt
      781 tagcgattat tcccagccgt ttttataacc tgtttagtcg ttgctggccg ctggaaaaac
      841 tgccttttcc gtccttaaat gaggagcaaa tagatttctc tatccactac aacaaattca
      901 gcctgcgtga tcccatcctg cacggggtaa tagatgttat ccgtaatgca ttttgaaaaa
      961 gtattgttac agccagatca cagcgcagaa cgataaaatg gcacaatcca acaacaattt
     1021 tacattttcg cgaccgcttt ggctgctttc aggtccgttt caatgatata ctgccagtcg
     1081 ttaattcaaa aatagttgat aattacaaca atctattgaa ttgaaacgct ttccttcgta
     1141 attcgcaact ggaacacgca cgctatgagt aaacccattg tgatggaacg cggtgttaaa
     1201 taccgcgatg ccgataagat ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa
     1261 gccctgctgc gcaagccgga atggatgaaa atcaagcttc cagcggactc tacacgtatc
     1321 cagggcatca aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc
     1381 tgccctaacc tggcggaatg cttcaaccac ggcacagcaa cgtttatgat cctcggcgct
     1441 atttgtaccc gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt agctcctgat
     1501 gccaatgaac cagtgaaact ggcgcagacc attgccgata tggcgctgcg ttatgtggtt
     1561 atcacctccg ttgaccgtga tgacctgcgc gatggcggtg cccagcactt tgcggattgc
     1621 attactgcca ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt gccggatttc
     1681 cgtggtcgta tggatcgtgc tctggatatt ctgactgcaa cgccaccaga tgtgttcaac
     1741 cataacctgg aaaacgtacc gcgtatttac cgtcaggtac ggcctggtgc agattacaac
     1801 tggtcgctga agctgctgga acgctttaaa gaagcgcatc cggaaatccc gaccaagtct
     1861 ggtctgatgg tgggactggg tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg
     1921 cgccgtcatg gtgtgacgat gttaacgctg gggcaatatt tgcagccaag ccgccatcac
     1981 ctgccggttc aacgttacgt tagcccggat gagttcgacg aaatgaaagc cgaagcgctg
     2041 gcgatgggct ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat
     2101 ttgcaggcga aagggatgga agttaagtaa tcctgtaata attacttaca tctgtctgat
     2161 aaaaaacccg ctttttg
//
LOCUS       DAZPBM010000317         2168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPBM010000317 DAZPBM010000000
VERSION     DAZPBM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.320"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(468..1019)
                     /locus_tag="ACHUZQ_004357"
     CDS             complement(468..1019)
                     /locus_tag="ACHUZQ_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1027867.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1818..2114
                     /locus_tag="ACHUZQ_004358"
     CDS             1818..2114
                     /locus_tag="ACHUZQ_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR1027868.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          628 a          467 c          422 g          651 t
ORIGIN      
        1 aggcttactt agtggaaaga gaggggatat gtgaccatag gggaacccct cctgttaaaa
       61 gactacttag aagtctgagg ttttagtaaa atctgtgaat atggcttctc agacgctctg
      121 taacgcattc taagcgattt taccccttta ttttaaggtg gttagtacat ccctataggt
      181 tacccctaaa aatggggtta aaactcgcta cagagctttc tatgagatta gcttctaatc
      241 gaatcaacta tgaggtttgg taggattgtg caaaatctgt acagtaactt ttcagaatct
      301 gaaaattttt ataaaaaatg actagccctc tttgaatctc atcttccccg aatcttcaaa
      361 aatatctgac agatgtctgt agctgctaag tacccgtcac accagctatg tgaccccctt
      421 ggcaaaaaag ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt
      481 ctgtaataag ttgctctaga gattcctaac tcttcctgaa tctgcaacct cgtcttacct
      541 tgtgaaataa gcgtctgtac agcctctgaa gaagctttta caggcctccc catatgtacc
      601 ccttcagctt tagcacgttc aataccagcc tttctacgct ctgctatcaa gcttctttca
      661 agctgtgcca acgttgacat aatgcctaac attgcttcac ccatagcgct tgataagtca
      721 aacccttctc taaggctgat aactgtcaca cctttagcct gtagcgcttt aaccgttgat
      781 aacacatcaa ttgtgtcacg tccaagacgg tctacagcta ccacaactac ggtgtcacct
      841 tctcgtgcat aagctaatag tctgctgaaa ccgtttctta aagaagcttt gacagaacca
      901 ctgacagctt catcagtgaa ccatttagca atgttgtacc ctgcctcttc aatttgcatt
      961 tgctggttag tgatggattg gtcatctgta ctcaccctgc aatatccaag tatcgccata
     1021 atgttcccct tagaagtctc aaaagttagt ctcataagtt tagctgtctc ataatccaaa
     1081 aacaaccttt tgagactact tgagggtgca ttctaccgtt acgttgaaag tgtctcaaaa
     1141 ggaagtcctt ttaagactgg ttatgaagga gctatgaggt tgattagggg ttggtacaag
     1201 cccaactaac cgaatcaact atgaagattt taggagggta actgtaacca tcactttcta
     1261 actttcacgt aggaaattgt taagtggttt gatagttaca ccatgtgacc aagcatctat
     1321 atatcgttat atcgccctgt atatagcact atatgcagtg tcgagagagg aacgactaac
     1381 acgatacagt ttaccctgaa aagagacttc acagattatt tatatatttc aataagttag
     1441 ggtaaaaagg gactataccg agactgtata gaatctgtgt agaattgcat agatacttgg
     1501 aaatctacta ttaaattgca tagggactca cctgatattg tcctgttttt aacaggtctc
     1561 ctagaaaacc gaatagactt cattcaaggg tcattgaagt cataatcatt tcagtagaaa
     1621 aaccaagtct cgattttaaa tgcaaatagc aatcatattc aataaggcaa gacgatcagg
     1681 atcagtgaaa atgtcatttt tggcgtaatt gaccttactt tatgcatggt cttaacaaag
     1741 acatcatctt tccgttgcag atcttcgccc tttcaaataa cgtactttac aactttcccg
     1801 aacaaggagt tgtgcccgtg ttgtatgtta tttacgctca agacaaagct gatagcctcg
     1861 aaaaacgcct ttccgttcgt ccggcacatt tagcacgttt acagttactg catgatgaag
     1921 gtcgtttgtt gacagctggt ccaatgccag cggtagacag taacgatcca ggggctgcgg
     1981 gatttactgg ctcaaccgtt attgctgaat ttgaatcgct ggaagcagct caggcctggg
     2041 ccgatgctga cccgtacgta gcggcaggtg tctatgaaca cgtttcagtg aaaccattta
     2101 agaaagtgtt ctgaatagag attcaatatt taagggcacg gtttttgcaa ccgtgttctt
     2161 gtttggcc
//
LOCUS       DAZPBM010000318         2145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPBM010000318 DAZPBM010000000
VERSION     DAZPBM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.321"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            156..2141
                     /gene="yjcS"
                     /locus_tag="ACHUZQ_004359"
     CDS             156..2141
                     /gene="yjcS"
                     /locus_tag="ACHUZQ_004359"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR1027869.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          574 a          567 c          523 g          481 t
ORIGIN      
        1 tccctcttca gcacaaatgc ctaataaaat ggatcgttca gctcacactt ccacttatta
       61 aagtcgcaaa ttcccaatta tgataacccc agctttaaat gacgtatttt gcatggatta
      121 catatttacg acataaatga taaatggaga tttacatgaa taactctcgg ttattccgtt
      181 tgagcagaat tgttattgcg ttaactgccg ccagcggcat gatggtaaat accgctaacg
      241 cgaaagagga agcgaaagcc gccactcaat atacccaaca ggttaatcag aattacgcca
      301 aatcattacc gtttagcgat cgtcaggatt ttgacgacgc tcagcgtgga tttatcgctc
      361 cgctgctgga tgaaggtatt ctgcgcgatg ctaatggcaa accatattat cgcggggaag
      421 attataaatt tgatatcaat gcgcccgcgc ccgaaaccgt taaccctagc ctgtggcgtc
      481 agtcgcagtt aaacggtatt tccggcctgt ttaaagtcac cgacagaatg taccaggttc
      541 gcggtcagga tatctccaac atcaccttta tcgaaggtga taccgggatt attgtcatcg
      601 acccgctggt gacgccaccg agcgcaaaag ccgcccttga cctttacttc caaaatcgcc
      661 cgcaaaaacc gattgttgcg gttatttata cccacagcca taccgaccac tatggcggcg
      721 tgaaaggcat tatctctgaa gctgatgtga aatccggtaa agtgcaggtt atcgcccctg
      781 ctggctttat ggacgaagcc atcagcgaaa acgtactggc gggtaatatt atgagccgcc
      841 gtgcacttta ctcctacggc ctgctgctgg cgcataaccc tcagggtaac atcggcaatg
      901 gtcttggcgt aacgctggca tcgggctacc cgagcatcat cgcaccgaac aaaaccatca
      961 ccaaaaccgg tgagaagatg attatcgacg gcctggagtt tgacttcctg atgaccccag
     1021 gtagcgaagc accagccgaa atgcacttct atattccggc cctgaaagcg ctgtgtaccg
     1081 ccgagaacgc cacgcatacc ctgcacaact tctacactct gcgcggcgcg aaaacccgcg
     1141 acaccagcaa gtggaccgag tatctgaacg aaacgctgga tatgtggggt aacgacgcgg
     1201 aagtcctgtt tatgccgcac acctggccgg tctggggcaa taagcatatc aatgattata
     1261 ttggtaaata tcgcgatact atcaagtaca ttcacgacca gaccctgcac ctggcgaacc
     1321 agggctacac catgaatgaa atcggcgaca tgattaaact gccgcctgca cttgccaata
     1381 actgggccag ccgtggctat tacggttctg tcagccacaa cgcccgcgcg gtgtataact
     1441 tctatcttgg ctattacgac ggtaacccgg ctaacctgca tccgtatggt caggtggaga
     1501 tgggtaaacg ttacgtgcag gcgctgggcg gttctgcccg tgtcatcaac ctggcgcaag
     1561 aagcgaacaa gcaaggtgat taccgctggt cggcagaact gctgaaacag gtgattgccg
     1621 ccaacccggg tgaccaggtc gcgaagaatc tgcaagcgaa taactttgaa cagctgggct
     1681 atcaggccga gtccgccacc tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg
     1741 aaggggtgca taagttcagc cacggcacca tcggttcccc ggacaccatt cgcgggatgt
     1801 cggtcgaaat gctgttcgac tttatgtccg ttcgcctcga tagcgcgaaa gccgcgggta
     1861 aaaatatcag cctgaacttc aatatgagca atggcgataa cctcaacctg acgctgaacg
     1921 atagcgtgct taactaccgt aaaacactgc aatcccaagc tgacgcctct ttctacatca
     1981 gccgtgaaga tctgcacgcc gtgctgaccg ggcaagccaa aatggcggat ctggtaaaag
     2041 cgaagaaagc caaaattatt ggcaatggcg cgaaactgga agaaattatc gcctgtctgg
     2101 ataatttcga tttgtgggtg aatatcgtaa ccccaaatta atgtc
//
LOCUS       DAZPBM010000319         2144 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPBM010000319 DAZPBM010000000
VERSION     DAZPBM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.322"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..988
                     /locus_tag="ACHUZQ_004360"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACHUZQ_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            999..1436
                     /locus_tag="ACHUZQ_004361"
                     /pseudo
     CDS             999..1436
                     /locus_tag="ACHUZQ_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1436..1813
                     /locus_tag="ACHUZQ_004362"
     CDS             1436..1813
                     /locus_tag="ACHUZQ_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027870.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          709 a          334 c          529 g          572 t
ORIGIN      
        1 tggggaaaca catctcgatt ttttgaagca atatggtgat tttgaaaatg gtattcctgt
       61 tcacgatacc attgccagag ttgtatcctg tatcagtcct gcaaaatttc acgagtgctt
      121 tattaactga atgcgtgact gccattcttc agatgataaa gacgtcattg caattgatgg
      181 aaaaacgctc cggcactctt atgacaagag tcgccgcagg ggagcgattc atgtcattag
      241 tgcgttctca acaatgcaca gtctggtcat cggacagatc aagacggatg agaaatctaa
      301 tgagattaca gctatcccag aacttcttaa catgctagat attaaaggaa aaatcatcac
      361 aactgatgcg atgggttgcc agaaagatat tgcagagaag atacaaaaac agggaggtga
      421 ttatttattc gcggtaaaag gaaaccaggg gcggctaaat aaagcctttg aggaaaaatt
      481 tccgctgaaa gaattaaata atccagagca tgacagttac gcaatgagtg aaaagagtca
      541 cggcagagaa gaaatccgtc ttcatattgt ttgcgatgtc cctgatgaac ttattgattt
      601 cacgtttgaa tggaaaggac tgaagaaatt atgcgtggca gtctcctttc ggtcaataat
      661 agcagaacaa aagaaagagc cagaaatgac ggtcagatat tatatcagtt ctgctgattt
      721 aaccgcagaa aagttcgcca cagcaatccg aaaccactgg cacgtggaga ataagctgca
      781 ctggcgtctg gacgtggtaa tgaatgaaga cgactgcaaa ataagaagag gaaacgccgc
      841 agaattattt tcagggatac ggcacatcgc tattaatatt ttaacgaatg ataaggtatt
      901 caaggcaggg ttaagacgta agatgcgaaa agcagccatg gatagaaact atctcgcgtc
      961 agtccttgcg gggagcgggc tttcgtaatc tttccctagg gagtcagccg aatgttggga
     1021 aggatttgac ggatgaagag aagaactgct tggccggcgg cagtacggga agcccgggag
     1081 gatggggacc agaagatgag aatagcgcaa gggatcgtat gcagcatgaa cggtataaag
     1141 atgaattgcg acagaaaatg ggaaaacccg acgttacaga ttcaaatttg aaaaatatta
     1201 tagacgatct ttatcggcca aatgcaaaag taggaagtgg aagcacagca gatgctgttc
     1261 gttatgaatt ggccacaggt gaaaaagttg gtggtagagg acatgtattg aaagcacagg
     1321 attatacaag ggctttacag gattggttgg ataagaatcc aacagcatct ccaggggatc
     1381 gagcagcagc agaaaacgtt cttcaggatt tacagaatgc tttgggaggc aagtaatgaa
     1441 ccaatatatt gctagttata tagaaaaaat ggcatcattt tccactgttt tgtctgagac
     1501 agtctcatta gctaatgaat actggcatcc agaaacgcca ccggtggtta tgttgttctc
     1561 tcaggttgga aaaacgattg taaaaaattt ttatgaaatg ggggatattg aaagagaaaa
     1621 aatatttaaa catgtcgaag atggtatggt ctcgcttgat gatgaattgg ccacagttgt
     1681 agctacaggg cttatagagg caatagtcac tgcgaccgat agcaattctt tactttggca
     1741 agatttaagt aaatttctgg gtgacaaatc aaagtctcat gcattagtgt ggaaaaattt
     1801 tggtcaggag tgatattatt caagaagtat taagctcgct attgagatct taatgcttct
     1861 tgagattaaa ttaaagttag gacgaagtaa ttgcaggtgg aaataaatcc atagtgacgt
     1921 gtggcaatgg ctattagatg ttatggtgtg atgggctttg gtgatttaag agctaagtta
     1981 gattgcctta tgaaaaagta tggttatata tttaatgatg tgtatcatat atttaaataa
     2041 aaaacggttc tctcttgtca agatagtaat aggatagact attgtgttga taaatataat
     2101 taagagtcta ctttgttgat tttagaagac tttggagcag ggaa
//
LOCUS       DAZPBM010000320         2136 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPBM010000320 DAZPBM010000000
VERSION     DAZPBM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.323"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            105..614
                     /locus_tag="ACHUZQ_004363"
     CDS             105..614
                     /locus_tag="ACHUZQ_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1027871.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            611..2029
                     /gene="phrB"
                     /locus_tag="ACHUZQ_004364"
     CDS             611..2029
                     /gene="phrB"
                     /locus_tag="ACHUZQ_004364"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1027872.1"
                     /translation="MTTHLVWFSQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          516 a          529 c          571 g          520 t
ORIGIN      
        1 tgataatatt tttaccaaca aaatacttcc ccgcaaaaac tctgccccat aaaattcatt
       61 taccatcgtc tacacttact cctttatacc gttcaggagc agtaatgaat ctacaacgat
      121 ttgatgacag caccctaatc cgtatctttg cccttcatga gttacatcga ctgaaagaac
      181 atggcttaac gcgcggggcg cttctcgatt atcacagccg ctataaactc gtctttctgg
      241 cgcattctca gccggagtac cgcaaacttg gcccgttcgt ggctgatatt caccagtggc
      301 aaaatctgga tgactattac aaccagtacc gccaacgcgt agttgttttg ctttctcacc
      361 ccgccaaccc gcgcgatcac accaatgttt tgatgcacgt tcagggttat tttcgcccgc
      421 atattgattc cacagaacgc cagcagctgg ctgcgcttat cgacagttat cgccgtggcg
      481 agcaaccact tcttgcgccg ctgatgcgta tcaaacacta tatggcgctt tatcatgacg
      541 cctggctttc agggcagcgt tatttcgaac tttggccgcg tgtgattaac ttgcgccatt
      601 caggagtttt atgactaccc atctggtctg gtttagccaa gatttacgtc tgcacgataa
      661 tctcgcactg gctgccgcct gccgcaattc gtctgcacgc gtgctggcgt tgtatatcgc
      721 tacaccacgc cagtgggcga cgcataacat gtcgccgcgt caggctgaac ttatcaatgc
      781 tcaactgaat gggctacaaa tagcgcttgc ggaaaaaggt attcctttat tgttccgtga
      841 agtggatgac tttgtcgcca gtgtcgaaat agttaaacag gtgtgcgcgg aaaacagcgt
      901 tacccacctg ttttataact atcagtatga agtgaatgag cgggcgcggg atgtggaagt
      961 tgaaagagcg ctgcgtaacg tggtgtgtga aggatttgat gacagcgtga tcctgccgcc
     1021 tggcgcggtg atgaccggta atcacgagat gtacaaagtc tttacgcctt ttaagaatgc
     1081 ctggctgaaa cggctgcggg aagggatgcc ggagtgcgtc gctgcgccaa aagttcgtag
     1141 tagcggatcg atagagccct cgccatccat tacgctgaat tatcctcgtc agtctttcga
     1201 tactgcgcat tttccggtgg aagaaaaagc ggcgattgcg caattacgcc agttttgcca
     1261 gaacggtgcc ggagaatatg agcaacaacg agattttccg gcagtggaag gcaccagccg
     1321 tttgtcggcc agcctggcaa cgggcgggtt atcgcctcgc cagtgcttgc atcgcttgtt
     1381 ggctgaacag ccgcaggcgc tggacggtgg ggtcggtagt gtctggctta atgagctgat
     1441 ctggcgcgag ttttaccgtc acctgataac gtatcacccc tcgttgtgta aacatcgtcc
     1501 aattattgcc tggacggatc gtgtacagtg gcagagcaat cccgcacatt tacaggcctg
     1561 gcaggaaggc aaaacgggat acccgattgt tgatgccgct atgcgtcagc ttaacagcac
     1621 tggctggatg cataacaggc tacggatgat tacagccagt tttctggtga aagatttatt
     1681 gatcgactgg cgcgaaggcg agcgatattt catgtcgcag ctgattgatg gtgatttggc
     1741 agccaataac ggtggctggc agtgggccgc ttcaaccgga accgatgcag cgccgtattt
     1801 tcgtattttc aacccgacaa cccagggcga gaaatttgat ctcgaaggtg agtttatccg
     1861 ccagtggcta ccggaactac gcaatgtacc ggggaaatcg gtgcatgagc cgtggaagtg
     1921 ggcgcagaaa gcaggtgtga agctggatta tccgcaaccg atagtcgagc acaaagaggc
     1981 gagagtacaa acgttggcag cgtatgaggc ggcgcggaag gggaaataac agacgcgtca
     2041 ggcaatcgag cccagatgcc ggatgcggcg tgaacgcctt atccagccta caaatttgtg
     2101 caaattcaat aaattgcagg aaatacgtag gcctga
//
LOCUS       DAZPBM010000321         2099 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPBM010000321 DAZPBM010000000
VERSION     DAZPBM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.324"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1446
                     /locus_tag="ACHUZQ_004365"
     CDS             <1..1446
                     /locus_tag="ACHUZQ_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1027873.1"
                     /translation="TAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHDERTH
                     HYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPE
                     VTWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQED
                     TGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAAQLEAEYIPERKLH
                     LYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSLRLPGQQYDEESGL
                     YYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHVNGDPSDFNQ
                     AVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIYWNSRASLFCSTEL
                     NSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKYENKEEARVITIIESRAAKT
                     LGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1458..1907
                     /locus_tag="ACHUZQ_004366"
     CDS             1458..1907
                     /locus_tag="ACHUZQ_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR1027874.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          610 a          383 c          610 g          496 t
ORIGIN      
        1 acggcgtggc cggataaccg catcgcggaa gatgcgcact atgtctatcg ctacgatgaa
       61 tacggcaggc tggcggagaa gacggaccgt atcccggaag gggttatccg gatgcacgac
      121 gagcgcaccc accactatca ctacgacagc cagcaccgcc tggtgttcta cacgcggata
      181 cagcacggcg aaccacaggt ggagagccgg tacctctatg acccgctggg ccgccggacg
      241 ggaaaacggg tatggcggcg ggagcgtgac ctgacggggt ggatgtcgct gtcgcgtaaa
      301 ccggaggtga catggtacgg gtgggacggt gacaggctga cgacgataca gaccggcacc
      361 acgcgtatcc agacggtata ccagccgggg agcttcacgc cgctcctgag aatcgaaaca
      421 gagaatggcg aacaggcgaa ggcgcggcac cggagcctgg cggaggtgtt gcaggaggac
      481 acgggtgtga cgctaccggc ggagctgtcg gtgatgctgg ggaggctgga gcgggaactg
      541 cgcgcgggag cggtgagtgc ggagagtgag gcatggcttg cgcagtgcgg gctgacggcg
      601 gagcagatgg ccgcgcagct ggaggcggaa tacatcccgg agaggaaact tcatctttac
      661 cactgcgacc accggggact gccgctggcg ctcatcagcc cggaagggga aacggcgtgg
      721 cagggggagt atgacgaatg gggaaacctg ctgggcgaaa ccagcgcgca gcacctccag
      781 cagtcactcc gtctgccggg gcagcagtat gatgaggagt cggggctgta ctacaaccgc
      841 aaccggtact atgacccgtt gcagggaagg tatatcaccc aggatccgat agggctgaga
      901 ggtgaatgga atctgtataa atatccatta aaccctgttc ggtttataga ttctttgggt
      961 ttaaaattcc atgttaacgg tgatccaagt gattttaatc aggctgttga gtatttaaaa
     1021 caagattcac aaatgaaaga aactatagat tttttatcaa gctctgaaga aactataaat
     1081 atagagtata ttgaaggaac caatgttaga tttaattcga ataatatgac gatatattgg
     1141 aatagtagag catctttatt ttgttcaact gaattaaact cgaaatcaca atcgcctgca
     1201 ttaggtttgg gacatgagtt tgctcatgcg cagtattgcc ttcttgataa ggaaaatttc
     1261 atggctcttt taagccgaac agataagaaa tatgagaata aagaagaggc aagggttatt
     1321 acaataattg aatcgcgtgc ggcgaaaact ttaggtgagt gtaccagagg agctcatagt
     1381 ggactgcctt tttatcgggt ggatggaccg ttgcagacga tgaaaataac gggtacgcca
     1441 gaatagtgag gttttttatg atgaaatatt tgatggtttt attgtctcta ttttctggtt
     1501 cggtattggg gatggggcga gtgaatgaat tgtgtggaat agatagcgtg aaaacgatag
     1561 agataattaa tcttccatcg tatgttacca cgttagttcc gcttagcaag gaaggtctta
     1621 atgaaatata cagatataaa gtggtggtga atgaaattag agatctttat gctggtaaga
     1681 taattgattt attgcaaatg aagtatttca gaaaagaaaa atataacaat ataagatggg
     1741 ggggtagtat catttcgaaa ggtaataata aatgtgaaat ttattttgat gcttttggtg
     1801 aatgtggaag tgtgaatggc attaacgtgt gttttgaaaa aaatgaaatg ataggttgga
     1861 ttaaaaaaga aatccctttg ctgtcgcaaa aaataggtgg gctttaaatg gtagggcaat
     1921 attcaaagac agtataggta actatttgta tggccatgta aggtaaaaga aaaggaataa
     1981 accatgagcg gaaaaccggc ggcgcggcag ggcgacatga cccgcaggga aagatcatca
     2041 gaagttcact ttttgtacta aataattcgc attttatgtt taaaaattga gatattcct
//
LOCUS       DAZPBM010000322         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPBM010000322 DAZPBM010000000
VERSION     DAZPBM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.325"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUZQ_004367"
     CDS             complement(60..1025)
                     /locus_tag="ACHUZQ_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR1027875.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUZQ_004368"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUZQ_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027876.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPBM010000323         2088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPBM010000323 DAZPBM010000000
VERSION     DAZPBM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.326"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHUZQ_004369"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHUZQ_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR1027877.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHUZQ_004370"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHUZQ_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR1027878.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZPBM010000324         2088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPBM010000324 DAZPBM010000000
VERSION     DAZPBM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.327"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHUZQ_004371"
     CDS             <1..314
                     /locus_tag="ACHUZQ_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027879.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="ACHUZQ_004372"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="ACHUZQ_004372"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1027880.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          518 a          509 c          484 g          577 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     2041 aaaaaatccc gccaacaata taagttttta taaaattatg gctttaga
//
LOCUS       DAZPBM010000325         2020 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPBM010000325 DAZPBM010000000
VERSION     DAZPBM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.328"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..354)
                     /locus_tag="ACHUZQ_004373"
     CDS             complement(<1..354)
                     /locus_tag="ACHUZQ_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1027881.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(354..848)
                     /locus_tag="ACHUZQ_004374"
     CDS             complement(354..848)
                     /locus_tag="ACHUZQ_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR1027882.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1206..1382
                     /locus_tag="ACHUZQ_004375"
     CDS             1206..1382
                     /locus_tag="ACHUZQ_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027883.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1524..1676)
                     /locus_tag="ACHUZQ_004376"
     CDS             complement(1524..1676)
                     /locus_tag="ACHUZQ_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027884.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1664..>2020)
                     /locus_tag="ACHUZQ_004377"
     CDS             complement(1664..>2020)
                     /locus_tag="ACHUZQ_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1027885.1"
                     /translation="DKNARELKLANATITDMQQRQRDADALDAKYTKELADAKAENDA
                     LRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQ
                     TALRTLQEYIRTQCLR"
BASE COUNT          498 a          555 c          476 g          491 t
ORIGIN      
        1 ggcgagtcgt tttttggagt gttcgcgggc tttttcctcc gtcatctgaa taatcagcat
       61 atcagcagga tcgcaaatca cgttgtaaat cacccagccg tcaatcaggc cgatagtctt
      121 gccggttcgt gccgggccaa caaatatcac tgcgtcgtat tcacgcgagg ccaggcagtt
      181 catcggctca ataacatacg gtgccaccag cggatcccac gggactgagt tccctgcccc
      241 catgggcacc cgcatatact gagcaacggc atcagcaacc cgcattcgtc tcggtgcgcg
      301 aaggatataa cctgaatcgg ttcgtgctgc ctttgcggtt tcctgattca gcattactcc
      361 tcctgctgta attcctcctc atcatccgca cctgcttcag tcacccgcag ggctatctga
      421 tcgcgcagat catcaataat ggactgaaca cggctcacag cggcaggctg cagaccgcag
      481 tcacgttcaa gaatatccgg taatgtctcc agcacctgca cgaccgcttt tgcccagatg
      541 gcaaactccc gtctgacatc actggccgga atgagttgtg ccgtttcctg ttcgaactta
      601 agacgctcac gttcagactg ataccaggct ttgcgctcat gcgcgtccat ttcgccttct
      661 gcaaccggcg gtggtaatgc cagaaatgcc gacacaatat caaccacccg ataaagcttg
      721 aggttgcttt catgcccccc tgcaacgggt agattttgca gccttgccgc agcagtctgg
      781 cgatgtacac ctgacagtgc cgccagttga ctgatattca gcgtcagatt ttttaactct
      841 cgatccatac ccgctccaga atgttttaaa catgcatctt gcgaacaact ttaggcaaac
      901 ggtgttagtg atgaacaaaa aacaatcaaa atcgacacca taaaaataaa accactgtaa
      961 tatcaatcca ttacagtagt ggtgatgacg aatgaaattt caaaaactag cctttttccg
     1021 cgccgctccc gccccgtggc aggccacccc accgggagga cccgtcagcc tgacagccat
     1081 gacgaacgtc tgatacagcg ccctgcatga atgacatcgg gataatccag aaaggaatag
     1141 catcgtaccc acaagaatca gtgtaagtgt cctgtttctt ccacccccgc acaggactgg
     1201 cgagcatgag ggacaaaccc gcgaaccata aacgcggtaa aaacccggtg tgcatcgttt
     1261 ttgattattc ccgcacactc gcgcagaagg agttccccgt cgggctacgg tcatggttaa
     1321 tacgggaata cggcgacgat acagcgcatg atgtgtcagg cttgaatacc tttatccgtt
     1381 aaaagggata tcagttaagt tatcccgtgt agggtataag ccattatcaa agccactctg
     1441 taggaagtgg cttttgtaat ggcaataaaa agccccgcga atgcgaggct aaatcctggt
     1501 atttgtaatg actggctctt atctcaacgc agccccttac cgcgcgcaaa atgctcaata
     1561 tcaagcatca gcaatgagat gtttaatctg gattcactcc agaagtgagc accaccctgt
     1621 ctacagagcc agatgtgaag gatgatgagt aaaattatcg ctatcatcga aggcattgcg
     1681 tcctgatgta ttcctgaagc gttctcagtg ctgtttggtc gcggataatt ccgtcccgga
     1741 taccgagaac gtttcgtcca gcaactggag agagttcgac ggtggcatca ttgcccatgc
     1801 cggaggcgct ggaggtttcg gctgaggatg gcacagggca ttttcctttg acgagcaccc
     1861 gaccaccatt atcaagcttg cgccgaagag catcattttc agctttcgca tcagctaact
     1921 ccttcgtgta tttagcatcg agtgcatcag catcacgctg gcgctgctgc atgtcagtaa
     1981 tggttgcgtt cgccagcttc agttctcttg cgtttttgtc
//
LOCUS       DAZPBM010000326         2013 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPBM010000326 DAZPBM010000000
VERSION     DAZPBM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.329"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHUZQ_004378"
     CDS             <1..275
                     /locus_tag="ACHUZQ_004378"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027886.1"
                     /translation="TYDRFNIQPINYKQNKNREHYMLYKIFTLNTKIKTTIITICALS
                     YNLTAQASPSYRPRDRVNKTMIELQTNGVERQLLDNSCGISSGVFK"
     gene            757..1104
                     /locus_tag="ACHUZQ_004379"
     CDS             757..1104
                     /locus_tag="ACHUZQ_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR1027887.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1124..1714)
                     /locus_tag="ACHUZQ_004380"
     CDS             complement(1124..1714)
                     /locus_tag="ACHUZQ_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027888.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1711..1971)
                     /locus_tag="ACHUZQ_004381"
     CDS             complement(1711..1971)
                     /locus_tag="ACHUZQ_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027889.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          519 a          523 c          461 g          510 t
ORIGIN      
        1 agacttatga tagatttaac atacaaccaa taaattataa acaaaataaa aatagggaac
       61 attatatgct ttataaaatt ttcacattaa acacaaagat aaaaacaact atcattacaa
      121 tatgtgcatt atcatataat cttacagcgc aagcttcccc gtcatacaga cctcgtgatc
      181 gagttaataa gactatgata gaactacaaa ctaacggagt agaacggcaa cttttagata
      241 attcttgtgg cataagttct ggggtattta aataaaatat aagacaggct gtctatctta
      301 cagacagcct ttttataata acagaaagaa tatatatcat acttaacgga agagataata
      361 tgaaaacagt acccgtataa acagcaagca atcccatttt tccaggtgta tcagcaaaaa
      421 aaccgctgtt ctagaaatca ggccgggtaa atttcagagc tgtatcttca atataccatt
      481 ttgcaaccgg atacaaaacc attccgcaga gagaaataca ccagaacagt aatctgtatt
      541 taaaatcata atcccatgac atatacagca tatatcctcc cgtcacccat ccccaccaca
      601 tattattaaa ataatatttt ctgttcatct gccagttttc ctttcttttg cacttttgtc
      661 agtgtactga tgcatgacaa cataaccaca gcatgataat aataatcaat aacaataagc
      721 tgtgtcacgt ttacatcatg gaacggaggt gctcctgtga cttttgagca gaaaaaagcc
      781 cgggccattg ccctgatgga cagcaaaaag atgtggcgga gcaattatgc ccctcctctt
      841 ctgcgtattc tctggcggct gggtatccgg cttccgccat taccctttat gccgttctgg
      901 caggtcacgg tgctgacggg gggcttatgg ggcatttcct ggggatgtgc aatgtggttt
      961 atctactggg ggccgtcagg aatggtggca ggtgaggcca ttattataag tatcacgggg
     1021 ggttttttgt ccggactgct catggcctct ttccactggt ggcgcaggaa agttaaccga
     1081 ctgccaccat gggatgatgt gtaaaccgtg cacaggcgtg ttcttacgga gcggcacttt
     1141 catttatctg tacgcgaacc tccattcctg catcgtgaca ggcctgcagc cactgcgcca
     1201 cttccagcgg atcgccctcc cggcgtacca cccagccttc tttattccat aactgcagac
     1261 aggtgctgcc gtcgagacgc accacaaaat ccccacggca ggcctgatac gttatgccgt
     1321 tcagctccac cgccagatca gtggcagaag tgccgcctgc gggcagacct gctgccggga
     1381 ccagcggcag tacgacgact tctgccgcac gctgttctgc ccggatgcgg tcctgttctg
     1441 ccagcagcag aggctgtgcc acggcccggc cggcgtccgt cagctccact gccagctgca
     1501 ggttcggagc gcgcagggta cgcagccagc cggcatcctc cagacgacgg caggaggcgc
     1561 gcaggtttgg cccgtagacc ggggcttccc cgctttgctc cagcacccgt tctatgtccc
     1621 gcgtcagcac gggtcctggc cgtttttcat caagggcagc cagcacaatc agcactctcc
     1681 gctgcagtgg cgacggtcgg cggctctgag tcatttccag tcatcctccg gccagatgag
     1741 ctttctgagg cgctgatggt cggcgtcaat gatctcctgt ctctttatgg cattcattga
     1801 ggtatagcca tttatggcca gttcatgcca gaagatcagc acggctccgg ctttggctga
     1861 ctcgcagaga taagttacgc cgtggctgtc cagattgcat tcccggatgc gtttttccgc
     1921 aaaccgggtc agttgttcat agctgagtga aaacgtcatt tcctgatcca tcctgcacgt
     1981 tccatctgat aatgttctga caggtaaaaa tca
//
LOCUS       DAZPBM010000327         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPBM010000327 DAZPBM010000000
VERSION     DAZPBM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.330"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUZQ_004382"
     CDS             <1..389
                     /locus_tag="ACHUZQ_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027890.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUZQ_004383"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUZQ_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1027891.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUZQ_004384"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUZQ_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1027892.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHUZQ_004385"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHUZQ_004385"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHUZQ_004386"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHUZQ_004386"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZPBM010000328         1980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPBM010000328 DAZPBM010000000
VERSION     DAZPBM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.331"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(358..1401)
                     /locus_tag="ACHUZQ_004387"
     CDS             complement(358..1401)
                     /locus_tag="ACHUZQ_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1027893.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1812..>1980)
                     /locus_tag="ACHUZQ_004388"
     CDS             complement(1812..>1980)
                     /locus_tag="ACHUZQ_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1027894.1"
                     /translation="LHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT          576 a          404 c          425 g          575 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccgaaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaataatat aataacaaaa
     1741 atcctccagc cttgtaggcc agaggataaa atacttaatt gaggaaaata tcagggaaaa
     1801 gtatttctga cttaactttc ccggctggag agtgaatcaa tgtaatcagc gtaccattgc
     1861 atcatttcac ggcggccatc gagatacagg gcatgattat aggtacccct gatggaattt
     1921 ttatccacat gagctaactg catttcaatc caggcggaat taaagccttg ttcatgcaga
//
LOCUS       DAZPBM010000329         1946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPBM010000329 DAZPBM010000000
VERSION     DAZPBM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.332"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            262..765
                     /locus_tag="ACHUZQ_004389"
     CDS             262..765
                     /locus_tag="ACHUZQ_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1027895.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(844..1095)
                     /gene="yecJ"
                     /locus_tag="ACHUZQ_004390"
     CDS             complement(844..1095)
                     /gene="yecJ"
                     /locus_tag="ACHUZQ_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1027896.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1210..1296)
                     /gene="azuC"
                     /locus_tag="ACHUZQ_004391"
     CDS             complement(1210..1296)
                     /gene="azuC"
                     /locus_tag="ACHUZQ_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1027897.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1559..1882
                     /locus_tag="ACHUZQ_004392"
     CDS             1559..1882
                     /locus_tag="ACHUZQ_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1027898.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          522 a          450 c          419 g          555 t
ORIGIN      
        1 atcaacaaga tacaaaaagc actatcatta aaattcattg cagttacatt gatttcatca
       61 atgaaatgta aaaatatata aacttgatga tttaagcatt ttcttatacc cgttcagacg
      121 ttattcttat ttcagatcat cgtcagaatt gactccacga tcacatttcg gaccggcaga
      181 aaggaattat tctgcaaaca gtaattatgg tgttttgatt tatcttgcac ctctccactt
      241 ctggatataa ggatattagg tatggcaacc gctggaatgc ttctcaaact caactctcaa
      301 atgaaccgcg agttttacgc atccaatctc taccttcacc tgagtaactg gtgttctgaa
      361 cagagtctga acggcaccgc cactttcctt cgcgcccagg cacagagtaa tgtgacccaa
      421 atgatgcgca tgtttaactt tatgaagagt gtcggcgcta cccccatcgt taaagccatt
      481 gatgttcccg gtgaaaaact gaactctctg gaagaactgt tccaaaaaac gatggaagaa
      541 tacgagcaac gttctagtac gttggcacag ttagccgatg aagcgaaaga actgaatgat
      601 gattcaaccg tcaatttcct gcgcgatctg gaaaaagaac agcagcatga tggtctgttg
      661 ctgcaaacca ttcttgatga agtgcgcagt gcgaaacttg cgggtatgtg ccctgtgcag
      721 accgaccaac atgttctgaa tgtcgtgtca caccagctgc attgatcatc atcggcgcta
      781 atgcattgcg ccgatgaagg ttttgagaaa ccgctgcctc atctgtttga agcagcggtt
      841 tttttaatgg gattcaccct gtggggtaaa cttgagttca ataagcgcga tggctttttg
      901 gattgcccgc atggtgaccg ggtctgcggc ggcgggatgg ttagtaaagt cgatattctt
      961 cagctgactg gacatttttt cacgaacttc aacgggcgcg attacatcga gaacatccag
     1021 aatttgtttg ataaccaact ggcaagcaac cacatcagaa accagttcct gatcggcatt
     1081 cagcggctgg gacatcgtaa actcctgata gcattttgaa agccgttata gtagcggctt
     1141 cacatcttca gcgatagtca catccaccgt catcaggaca caaaaaaacc tgccggagca
     1201 ggttttttgt tatcggaaca tattgcctgg cggtacgtct ttgaacgtct tgcaatagtt
     1261 attgaacata cttttcagga ttttgcgcag tttcatcgcg gcactccgac catttgttat
     1321 acaggtgtta ttgtctttgc gcttataata tgacaaccat cacaaaaatc aatctttatg
     1381 tgatacaaat cacataaata cccctttaat gttataaaaa tgataatcaa aaaacagccc
     1441 ccctatttct gacacctaca gatggcaaga aatagcgcct gccaggcgtc ttttccggcc
     1501 attgtcgcag cgctgtaacg cgtaaaatag tgctttctct tactcttctg gctggaccat
     1561 gagacttctg attctgactc tttcattaat aacgctggcg gggtgtacgg ttactcgcca
     1621 agcccatgtg agcgaagttg acgcggcaac aggtattgta cggttggttt atgaccaggc
     1681 ttttttgcag catgcccata ccgatcgtta tgtcagtcgc gggatcgccg atcgcgcatg
     1741 ccagcaggaa ggctataccc acgcggtccc ctttggtcag ccagtaggca actgtagcct
     1801 ttttgcgggt tccttatgtt tgaatacgga attcactttg tcatatcagt gccatcattc
     1861 cgccttccct gtctttctct aaatatttta atcagcgagg ggatcttcgc tgattaaaga
     1921 aatagcggta atgcgtttta ttccca
//
LOCUS       DAZPBM010000330         1860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPBM010000330 DAZPBM010000000
VERSION     DAZPBM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.333"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(89..643)
                     /locus_tag="ACHUZQ_004393"
     CDS             complement(89..643)
                     /locus_tag="ACHUZQ_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1027899.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="ACHUZQ_004394"
     CDS             complement(640..930)
                     /locus_tag="ACHUZQ_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1027900.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="ACHUZQ_004395"
     CDS             complement(930..1529)
                     /locus_tag="ACHUZQ_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1027901.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAAQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="ACHUZQ_004396"
     CDS             complement(1601..1813)
                     /locus_tag="ACHUZQ_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027902.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          460 a          496 c          379 g          525 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgcgct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
//
LOCUS       DAZPBM010000331         1837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPBM010000331 DAZPBM010000000
VERSION     DAZPBM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.334"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(37..582)
                     /locus_tag="ACHUZQ_004397"
     CDS             complement(37..582)
                     /locus_tag="ACHUZQ_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR1027903.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            971..1204
                     /locus_tag="ACHUZQ_004398"
     CDS             971..1204
                     /locus_tag="ACHUZQ_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR1027904.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1262..1672
                     /locus_tag="ACHUZQ_004399"
     CDS             1262..1672
                     /locus_tag="ACHUZQ_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR1027905.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT          551 a          455 c          372 g          459 t
ORIGIN      
        1 gagcgaagcc cggcgcggac aaaatgccgc agcctgttaa cctgactgtt cgatatattc
       61 actcagcaac cccggtatca gttcatccag cgcggctgct ttgttcatgg ctttgatgat
      121 atcccgtttc aggaaatcaa catgtcggtt ttccagttcc ggaaaacgcc gctgcaccga
      181 cagggggatc ccgtcgagaa tactggcaat ttcacctgcg atccgcgaca gcacgaaagt
      241 acagaatgcg gtttccacca cttcagcgga gtctctggca tttttcagct cctgtgcgtc
      301 ggcctgcgca cgcgtaagtc gatggcgttc gtactcaata gtccctggct ggagatctgt
      361 ctcgctggcc tgccgcagtt cttcaacttc ccggcgcagc ttttcgttct caatttcagc
      421 atccctttcg gcataccatc ttataacggc ggcagagtca taaagcacct cattaccctt
      481 gccaccgcct cgcagaacgg gcattccctg ttcctgccag ttctgaatgg tacggatact
      541 cgcaccgaaa atgtcagcca gctgcttttt gttgacttcc attgttcatt ccacggacaa
      601 aaacagagaa aggaaacgac agaggccaaa aagcccgttt tcagcacctg tcgtttcctt
      661 tcttttcagg gggtgtttta aataaaaaca ttaggttacg gcgaagaaga acggaaacgc
      721 cttaaaccgg aaaattgtca taaatagcga aaacccgcgc gccttccgcc ccgtaacctg
      781 tcggatcgcc ggaaaggacc cgcaaaatga taataattat catctacatg tcacaacgtg
      841 catctacgcc atcaaaccac gtcaaataat caattatgac gcaggtatcg tattaattga
      901 tctgcatcaa cttaacgtaa aaacaacttc agacaataca aatcagcgac actgaataca
      961 gggcaacctc atgtcaacga agaacagaac ccgcagaaca acaacccgca acatccgctt
     1021 tcctaaccaa atgattgaac aaattaacat cgctcttgtt caaaaagggt ccgggaattt
     1081 ctcagcctgg gtcattgaag cctgccgccg gagactgtgc tcagaaaaaa gagtttcgcc
     1141 tgaagcaaac aaagaaaaga gtgacattac tgaattgctc agaaaacagg ttagaccaga
     1201 ttgaagcaat ttagataatc gtgcagacta cgccccctca tatcacatgg aaggtactac
     1261 aatggctcag gttgccattt ttaaacaaat attcgataaa gtgcgaaata atttaaacta
     1321 tcactggttt tattctgaac taaaacgtca caatgtctca cattacattt actatttagc
     1381 cacagagaat attcatcttg ttcttgaaaa cgataatacg gttttaataa aaggacaggg
     1441 taaggttgta aatgtaagat tttcaaaaaa taaatgcctt atagaagcca ccttaaaagg
     1501 attcaaatca ggagagttat cattttacga atacaggaaa aatcttgcta cagcaggggt
     1561 tttcagatgg attacaaata tccacgaaaa caaaaggtat tactatacct ttgataattc
     1621 attactcttt actgagaaca tacagaacac tacacaaata tttccgcact aaatcataac
     1681 gtccggtttc ttccgtgcca gaaccggact cgctggcatg atgaaatatg tgtacccggt
     1741 aaccccggtg tgcatcgttt ttgattattc ccgcacactc gcgcagaagg agttccccgt
     1801 cgggctacgg tctctgttaa tacgggaata cggcgac
//
LOCUS       DAZPBM010000332         1780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPBM010000332 DAZPBM010000000
VERSION     DAZPBM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.335"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            119..307
                     /locus_tag="ACHUZQ_004400"
     CDS             119..307
                     /locus_tag="ACHUZQ_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027906.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQCEWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            317..1516
                     /locus_tag="ACHUZQ_004401"
     CDS             317..1516
                     /locus_tag="ACHUZQ_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1027907.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGCLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDIS
                     LLRYMPA"
     gene            complement(1687..1780)
                     /locus_tag="ACHUZQ_004402"
                     /pseudo
     CDS             complement(1687..1780)
                     /locus_tag="ACHUZQ_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          409 a          452 c          536 g          383 t
ORIGIN      
        1 aaccacggca gcctgacggc tgcaaattga aaaccgcccc tgttcgtcct gttgttgccg
       61 cacgctccgc agaatatgta tgctctgagg acctgtttta ttaaggactc atcctcttat
      121 gttgacccgc aaaagtatag ataccgttct gctctctgtt ggtgctgaga agctctcaca
      181 atgtgaatgg gactggatga agatgctcaa acccatggac ccaccacctg cgatggtcgc
      241 ggcctccatc ctggagcgcc gtggcgacac agcagccctt acccggctac aggataccgg
      301 gggttaatcc gtcctcatgt tgtccgcttt tactccccgc ccgctgaaac gtctgttcac
      361 ggccaaccag tgctggacat ccttcctgga tgcgggcggt ctgcgcgata tcgaggttga
      421 ggctgtcaca aaaatgctgg cctgcggcac gcggatactg ggagtgaagg aatacaactg
      481 cgataaacct gagtgccccc acgtcagata cgtcactaac tcatgcggca gccgtgcctg
      541 cccgtcctgc ggaaaaaagg ccacagacct gtggatagcg acacagctga atcgtcttcc
      601 tgactgcgac tgggtacatc tggtcttcac cctgccggac acgctgtggc cggtgttcga
      661 aagcaaccgg tggctgctga atgacgtgtg ccgtctggcg gtggagaatc tgctgtatgc
      721 cgcccggaaa cgggggcagg aacccggtat cttctgcgcc atccacacgt atggccgtcg
      781 tctcaactgg catccgcatg tacatgtgtc tgtaacctgt ggatgtctga ataagcatgg
      841 tcagtggaaa aagctgagct tcctgaaaga cgcgatgcgt tcgcggtgga tgtggaatat
      901 gcggcaacgg cttctgaaag cgtggtcaga ggggctggcg atgccggagt cgttgtcaca
      961 tatcacgacg gaatcacagt ggagaagcct ggtgctgaaa gccggaggaa aatactggca
     1021 tgtgtacatg tcgaaaaaaa cggccggcgg gaggaatacg gcgcgctacc tgggtcgtta
     1081 tctgaagaag ccgccgatag cggcctcccg gctggcacat tacaacggag gggcgagcct
     1141 gagcttccgt tacctggacc acaaaacggg agaaacggcg acggaaacgc tgacacagcg
     1201 tgagctggtc gcgaggctga aacagcacat cccggagaag ttttttaaga tggtgaggta
     1261 cttcgggttc cttgccaacc gggtgtgtgg agagaagctg ccgcaggtgt accgtgctct
     1321 ggggatggat aaaccggaac cagtggcgaa agtgtgctat gcacaaatgg tgaaacagtt
     1381 cctgagtcgt gacccgttcg aatgcgtgct gtgtggcggc cggatggtat accgccgggc
     1441 cattgcgggg ctgaatgtgg aggggctgaa gaaaaacgcg cgggatatca gtctgctgag
     1501 gtatatgccg gcctgacaca ggctaagtgt gcccgggaat cggtgcaagt ataaaaaaca
     1561 gcctgttgcg gtataagaat cgaccatgca gagagagaaa acaccggagt ggtggggaaa
     1621 aacagaaagg ctcacgtggc ataaggcgcg gacaggggta tcgcttagtt tttcaatttc
     1681 ctatacgtca agctgtgcac gtccatccga tgccattttc tcatttgact ttcgggggat
     1741 tactgccttt ataccgttat ttttcagctc gttgcgcaat
//
LOCUS       DAZPBM010000333         1745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPBM010000333 DAZPBM010000000
VERSION     DAZPBM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.336"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..415)
                     /locus_tag="ACHUZQ_004403"
     CDS             complement(<1..415)
                     /locus_tag="ACHUZQ_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1027908.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
     gene            complement(431..1105)
                     /locus_tag="ACHUZQ_004404"
     CDS             complement(431..1105)
                     /locus_tag="ACHUZQ_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1027909.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            complement(1185..1727)
                     /locus_tag="ACHUZQ_004405"
     CDS             complement(1185..1727)
                     /locus_tag="ACHUZQ_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1027910.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          497 a          388 c          313 g          547 t
ORIGIN      
        1 tttccagttc ttccacccag gcctgcggga cactgaaatc gagacgaaaa accccgatat
       61 cccaggtata gctcccaccc tgaacaagtt gctcaaatgt taaacattgc ttaccgctgg
      121 cgaagttatc gctattaatg cctaaccgct tgataacttc tcttgataaa catgtttctt
      181 gcgggttgtc tttaacaata atctcatatt tcccgcgcca ttgcttattg acataaatat
      241 cgatgtcata ctgccccggt aagggttgat tatcatcaag acgaatattt gcaacctgct
      301 ggtctttcat tccacctatc ataaaatggg tatcaaaggt ttcttcagct gcataagctt
      361 caatgccgag caataacgct acgattgctg atgcaagtgg ggtcattctc aacataggca
      421 gctcctgcaa ttaaattttg tcactaatat agttgccatg gtcatcgata atggtcagat
      481 gccagttatt tgcattatta cttttgacat taacactctg actttctaag ggggcaatca
      541 taatagtttc ataattgact ttgacattat tagctttgac atccgaaatc gtcacccaat
      601 tagctgagtc attttttatc accaaaccat tgccactgcg atttaccagc aattttttaa
      661 atgtcgcttt atttaccgga gcaatacccg ctggtcggta aaacaactta attctgtttt
      721 gcatcgcaaa cttcagtgca ttcttacctt cttgctctgg actatttggt ggaatatcca
      781 gaacattcag ataaaaaatg ctttctttat tagtgggcag tttattcggc ataattttga
      841 tttttacttg ctgcccggaa tttgccccta tttttgccac tggtggcgtt aacatgaaag
      901 gaacctgaat tttttctggt ggtaatgacg tatcgccatc atcaatccac gcctgcagca
      961 gcgaagaacg gtttccctgg ttcatcaact gcaccatcac ttctttattt tctgccgggt
     1021 aaataatgcg cgtcccgtag ataacaattc cggcatgtgc aggaaggacc atagaaaaaa
     1081 tgagtaaaga taataatcct ttcattttaa tgcctcttaa attaaaaggc cgccatggga
     1141 tagcggccat gtttattacc tggagaaact tacaatttta tttattaatc ctgcgccaca
     1201 cgcataacca gagaagataa cacatcacct gattgcactg agttatctgt cagtttcagg
     1261 tattttgctt taaacggaat agcatattga ccttccgctg ttttataact ctcaatgtta
     1321 aaaggtgcag aaacatccag gacatcgctg ttatcaccta aatagagata gtaacccacg
     1381 gtatcgttgc tactggctat cgccttatcg ttaccggtta ttttatcgtt ggtaatcaac
     1441 gagagtttta caccagcaac ttctttcggg cagctaattt ttaacatgct gctggttaca
     1501 ccgactgcat catcataggt agaatattta gctttacctt caaagacagt ggttggtaca
     1561 gtcggcatat taatacttac tgaatcacca cccgcttcga atttacacgg agattctgca
     1621 atattcccct taatagttaa tgttccggta tcaacgtctg cagcaaatac tccagcgctc
     1681 ataaaaaaag aagaaaagac agcggcagca ataattgaac gtttcataat atgttcctta
     1741 ataaa
//
LOCUS       DAZPBM010000334         1696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPBM010000334 DAZPBM010000000
VERSION     DAZPBM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.337"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHUZQ_004406"
     CDS             <1..547
                     /locus_tag="ACHUZQ_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027911.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
     gene            623..>1696
                     /locus_tag="ACHUZQ_004407"
     CDS             623..>1696
                     /locus_tag="ACHUZQ_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1027912.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLS
                     ALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAA
                     EGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPD
                     TLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQV
                     RGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          349 a          443 c          576 g          328 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc agggcgacat
      661 gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag gtgcgccgac
      721 gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg tgaacccggt
      781 gaacccggtg ctgggtgcga aggtgcttcc gggcgagacg gaccttgcgc tgccctgccc
      841 gctgccgttt attcttttcc gtgcctacag cagctaccgg accagaacac ccgcgccggt
      901 gggggtgttt ggtcccggct ggaaagcgcc cttcgatatc cgcttacaga tacgcgatga
      961 aggcctgata ctcaacgaca gcggcggcag gagcattcac tttgagccgc tgtttcccgg
     1021 cgagataagc tacagccgca gcgagtcact ctggctggcg cggggcggag tggcggcgca
     1081 gcacagttcg cagccgctaa gcgcgctctg gcaggtgctg ccggaagatg ttcgcctgag
     1141 tccgcatgtg tacctggcga caaacagcct tcaggggccg tggtggatac tcagctggcc
     1201 ggagccgccc gcataccggg tactgacggt cgtggtggat ggcttcgggc gctcactgac
     1261 tttccaccgg gcggcggagg gtgacgtggc gggcgcggtg acgggggtga cggacggcgc
     1321 gggccgccgt tttcacatgg cgctgagcac gcaggcgcag cgggcggaag cgtcccgtaa
     1381 acagcgcgcc tcttccttat cttccccggc cagtccccgt tctgtttcct cttctcaggt
     1441 tttccccgac acgctgcccg ccggtacaga gtacggtgcc gataacggta tccggctgga
     1501 ggcggtatgg ctgacacacg acccggcata cccggatgaa cagccgactg cgccgctggc
     1561 gcgctacacg tacacggccg gtggtgaact gcgggcggtg tatgaccgca gcgggacgca
     1621 ggttcgcggg tttacttatg atgcggagca cgccgggcgg atggtggcgc accattatgc
     1681 ggggaggccg gagagc
//
LOCUS       DAZPBM010000335         1668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPBM010000335 DAZPBM010000000
VERSION     DAZPBM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.338"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..294
                     /gene="iraM"
                     /locus_tag="ACHUZQ_004408"
     CDS             <1..294
                     /gene="iraM"
                     /locus_tag="ACHUZQ_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1027913.1"
                     /translation="GISFSVIWSKIKLIIWYQSDAFLPPESIFTLTHTGIMLNNKVLP
                     VTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQQSP"
     gene            complement(575..706)
                     /locus_tag="ACHUZQ_004409"
     CDS             complement(575..706)
                     /locus_tag="ACHUZQ_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR1027914.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1206..1282)
                     /locus_tag="ACHUZQ_004410"
     tRNA            complement(1206..1282)
                     /locus_tag="ACHUZQ_004410"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1246..1248),aa:Arg,seq:tct)
     gene            complement(1383..1458)
                     /locus_tag="ACHUZQ_004411"
     tRNA            complement(1383..1458)
                     /locus_tag="ACHUZQ_004411"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Ile,seq:cat)
     gene            complement(1561..>1668)
                     /locus_tag="ACHUZQ_004412"
     CDS             complement(1561..>1668)
                     /locus_tag="ACHUZQ_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1027915.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          519 a          429 c          305 g          415 t
ORIGIN      
        1 ggaatatctt tttcagtcat atggagtaaa ataaaattaa taatctggta tcaatcggat
       61 gctttcttac ctcctgaaag tatatttaca ctgactcaca caggcatcat gctcaataac
      121 aaagtgctac ctgtaaccat ttacaacgta gtaccattca ataaaacatt ctggaattca
      181 atcaaaaata gccaggaatg ccctacaaat acagataacg tattgaatga atgctttaat
      241 aaccgttgca ctctgcaaat atgtccttat ggactaaaac aacaaagtcc ataaggagtt
      301 tactcacatc tgacaaaatc aatataaaca gcccctccgg agaggggctg gagagtggcg
      361 ctatgtgcca ttgcatggtg ccgggtgcct cccggtgaat tcagtaccag cacctgaatc
      421 cgcgattatc ccatatacct actcgctgat tgcccctccg cacaggggga ttcaccatgc
      481 cagtttcttt taacaaactc cccgcaaacc agacaacagt caaccgcctg aattgtgaag
      541 tatttaaaaa tttctcccgc taactgatac ccggctaaca gtctggcgtt ttctttttca
      601 gcaacgggaa agcaacaacc accacacccg ccaccagcac accgtcagcc agcactgaca
      661 ttatccggct gctgcaatgc cattcacaaa aacagtaagc aatcactttt taccgtaaca
      721 ggtgataatc cagatatgta tctaccccag atgagtaatc cgaagttcat ccataccaca
      781 ggtcctggct attctgttgt actcctgaac aagagcaaat aattctgaat tagcaaccat
      841 gaactcatcg caaaccctct gtatagcatc actattcaga ataataacgt ctcttcccga
      901 aagacgatca ggagtacaga acaaaactgt caaacggctg aaggcctttg ctcgtgctgc
      961 attgactata tcaatacgct gcctaaggat gaaacacccc gacgcctcat caatattcac
     1021 tctacccaca ccatatgaat gataaatatt taatgctgaa aaaaccatta gaccgtataa
     1081 caaacactca atcaatactt aacagaactt ttatttttga caaacataaa atattttcaa
     1141 caatatcctg agccaggtat atttcagtat aaggctctgc cggaaggaat ctggaagaat
     1201 gaatatggcg cgctgtactg gattcgaacc agtgaccgat tgcttagaag gcaattgctc
     1261 tgtccggctg agctaacaac gctgaatacc gataatggac cgccatcggg gacccgcccc
     1321 cgcaccaaca accctgttat catgtcgtct gctcttcctg ataagctaat ggcggtttgt
     1381 gatggtggcc cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca
     1441 ccactgagct aaagggccgg gagcagaata ataacggtcc gtaattaatt ccgcaataaa
     1501 aaacccgctc ggcggcgggt tgtagaaact cttctaacgt caggcataaa acgcccatag
     1561 ttatgacgaa tttaccacag attccggaaa aatcaaccta gttacctttt ttaactgccg
     1621 ctcagcccat gcttcttcaa tatcaaaccg ggtcaccagc gcatcata
//
LOCUS       DAZPBM010000336         1630 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPBM010000336 DAZPBM010000000
VERSION     DAZPBM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.339"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..94
                     /locus_tag="ACHUZQ_004413"
     CDS             <1..94
                     /locus_tag="ACHUZQ_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1027916.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            105..293
                     /gene="dicB"
                     /locus_tag="ACHUZQ_004414"
     CDS             105..293
                     /gene="dicB"
                     /locus_tag="ACHUZQ_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR1027917.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            290..481
                     /locus_tag="ACHUZQ_004415"
     CDS             290..481
                     /locus_tag="ACHUZQ_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR1027918.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            575..>1630
                     /locus_tag="ACHUZQ_004416"
     CDS             575..>1630
                     /locus_tag="ACHUZQ_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR1027919.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          362 c          391 g          379 t
ORIGIN      
        1 ccggttttat ctggaagaac agggatggac tcttgttaac cggggcgcaa ttaaaaatat
       61 gactgtagag caattactca aaattcattg ttgaggacgc gataatggaa acgttattac
      121 caaacgttaa tacgactgaa ggttgttttg aaattggtgt cactatcagt aacacggtat
      181 ttactgaaga tgccattaac aagagaaaac acgaacggga gtcattaaat aaaatatgca
      241 ttgtttcaat gctggctcgt ttgcgtctga tgcaaaaagg atgctggcga tgaatacagc
      301 atttgcactc gttctgaccg ttattcttaa tacgggcgaa ccagctgaac tggttaccgg
      361 agtatacaac tccatgaaag aatgcgtggc tgctgcagct gaacagaaaa ttcccggtaa
      421 ctgttatccg gttgataaaa tcattcacca ggataataaa gaagtcccgg caggtcttta
      481 aagcagccac gtaataaata tccggtttca tttttatatg ccagcaatgg cagggatttg
      541 ttcaccctta aatctgtaat gaggttaaaa caaaatgagt aaagtcttta tttgcgccgc
      601 cattccggac gaaaaggcaa caaaagaaga aggcgctaaa cctgtagcca ctgccattga
      661 agccggcgac gaacgccgcg cccgcgcaaa atttcactgg caattcctgg aacattatcc
      721 ggctgctcag gactgcgctt ataaatttat tgtctgcgag gataaacctg gtataccccg
      781 ccctgccctc gattcctggg atgctgaata tatgcaggaa aaccgctggg atgaggagtc
      841 tgcttccttt gtcccggttg agactgaatc cgaaccgatg aacgtcactt ttgacaagct
      901 ggcccctgaa gtacagaacg ctgtcatggt taagttcgac acatgtgaaa acatcaccgt
      961 tgatatggtg attagcgcgc aggaactgtt gcaggaagac atggcaacat tcgacggaca
     1021 tatcgttgaa gcgttgatga aaatgccaga agttaacgcc atgtgtccgg agattaaact
     1081 gcatgccatc gggtggatta agcataaatg taagcctggt gcaaaatggc cagaaattca
     1141 ggcagagatg cgcatctgga aaaaatgtcg cgaaggtgaa cgcaaggaaa ccggaaaata
     1201 cacgtctgtt gttgatctcg cccgtgccag agccaatcaa cagcacactg aaaattcaac
     1261 aggaaaaatc agcccggcca ttgctgccat tcatcgcgaa tacaagcaga catggaaaac
     1321 actggatgac gaactggcct acgctctctg gcctggtgat gtggatgccg gaaacattga
     1381 cggcagcatc catcgctggg caaaaaatga agttatcgac aacgaccgcg aagactggaa
     1441 gcgtatctcg gcatcaatgc gcaaacagcc tgatgccctt cgctacgacc gccagactat
     1501 ttttggcctt gtccgtgaac gtccgatcga cattcacaaa gaccctgtgg cactgaacaa
     1561 atacattact gaatacctga ctacaaaggg cgtgtttgaa gatgaaggaa caaatcagag
     1621 cgcaactgat
//
LOCUS       DAZPBM010000337         1625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPBM010000337 DAZPBM010000000
VERSION     DAZPBM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.340"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..1436
                     /locus_tag="ACHUZQ_004417"
                     /pseudo
     CDS             1..1436
                     /locus_tag="ACHUZQ_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
BASE COUNT          460 a          419 c          394 g          352 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agcgaactga ggtgtactgg caatagcgga
     1561 cactaccatt tgttcttttt ttaagcagcc atctgatgat atttttccct gaaggctgcc
     1621 gggga
//
LOCUS       DAZPBM010000338         1624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPBM010000338 DAZPBM010000000
VERSION     DAZPBM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.341"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..1624
                     /locus_tag="ACHUZQ_004418"
                     /pseudo
     CDS             1..1624
                     /locus_tag="ACHUZQ_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          503 a          317 c          334 g          470 t
ORIGIN      
        1 accttactga taatattatc aaactgtcga ataaaatagg tcagcgtgtt tatactgttg
       61 tctctgaaat gtcgattgaa gcatggataa caaaagaacc gcagcctttt gtttatcgcc
      121 gtagcggcgc ttaccagaaa ctcaccattg gctcagactg gggtcaactg tttgactgtg
      181 cctggatgcg agtttatggt aaacgtccag cagataaatt tagacacaaa ctcgtcgctc
      241 tcgttgatat cggtggcgaa ggtcttattg tggataagaa tggttcaccc atctgcggca
      301 tcacgaataa agcatcatct tatggcgtac cgccagataa accaggtaaa tgggtggttg
      361 acctttctct cgttagtgaa aatgacgagg ttgagttttg gattgatgct gcctgcaacg
      421 atttatttgg ctatgttact aatggaggga ttattactga tgttcatatc gcgacacgta
      481 atcaactact gaaatccctt tattatgata ttgaggtgct tttcgactgg attaatgatg
      541 gtcagaagtt tgaatccata catcctaaag gtatcacccc agaacaattt attgccaaaa
      601 gaagtgagcg tactgacgaa atcataagta ctctcgaata tattgataat acgttaatta
      661 cctttagcaa tgaagaaata attaaatgtc aaattgcaat ccaatccata atcaataaaa
      721 acaataatcc ttcagagttc aggattatgg ctactggtca tgcccattta gatatagcgt
      781 ggatgtggcc tttaagagaa ggacgcagaa aagcaataag aacatttgcc accgcccttg
      841 caaacattga aaaatatcct gattacattt ttggcgctag tcagtaccaa ctttttcact
      901 ggataaaaaa agattatccc tatttcttcg aaaagttaaa aaaacaaatc gccgcaggcc
      961 gctttgagtt acaaggttgc ttctgggtcg aatgcgattt aaatctggta agtggtgaat
     1021 cgctgataag gcaaatcatg catgggacac gattcacact gcagaatttt tcaaaaaaga
     1081 taaattatgt ctggcaaccg gatgtctttg gattccccgc aacgcttcca caaattctca
     1141 aaaaatcagg cattaactat attgcatcgc aaaagctctc acaaaacaag attaataaat
     1201 ttaataatta ccttttccgc tggcagggcc tggatggctc agaaattctg atgcataact
     1261 ttcctgaaga cacttatgat agccgagcac gtgctcgctc tttagaatat attgaacaaa
     1321 actataatga aaaagagatc tgtccctatg cgcttatggt gtatggtgta tggtgtcggt
     1381 gatggcggcg ctggccccgg tgaggaacat atagaacgat taacacgtat acgcaatatt
     1441 gatggcttac ctcatgttga tttcagccgt gttgataagt tcttcactta cgccgacgcc
     1501 tttcgcgaaa gcttgcccat tatttcaggt gaactttatt tcgaggctca tcaagggtgc
     1561 tttacatcag agtcagcaac taaagctcat aatcgaaata tggaaaataa attacatgat
     1621 gctg
//
LOCUS       DAZPBM010000339         1616 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPBM010000339 DAZPBM010000000
VERSION     DAZPBM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.342"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(75..>1616)
                     /locus_tag="ACHUZQ_004419"
     CDS             complement(75..>1616)
                     /locus_tag="ACHUZQ_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFR1027920.1"
                     /translation="LLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVN
                     AWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIA
                     DIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDAS
                     GYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQS
                     AAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAAD
                     RIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPSAFSLTPDGKLTAK
                     NADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTE
                     TPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGV
                     LIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAAS
                     GISIR"
BASE COUNT          360 a          457 c          372 g          427 t
ORIGIN      
        1 gcataattac ctctcgcttt tctgcaataa aaaaggcgtc attcctgacg ccatttattg
       61 gggttataaa tatttcaacg aatactgatg ccggaagccg cttttttggt cacaatcacc
      121 gtacagtcgg tgatattgcc tgccccctga ttgcctttct ggaaaatctt aaactccaga
      181 gtgacgctac caccaccact aggcatatca ataactgcac tgtaactacc gggaatggca
      241 cctttagttt ctctggatgc gattaatacg ccgtttttgc gaacttcaaa accataaccc
      301 gtgtatcgcg tgcctcctgg gttatttccg ctccccggat cgtcatacgc tataccgtta
      361 aaaataatgg gcggaataat aatctggcgg tcaaagttat gatcatcgct tatggtgact
      421 gtaaccgtcc cgtttggtgt ttccgtgtta ccccacgtac cagcctgttt cggaaatgat
      481 ttggatacag ctttaacgaa gtcacctctg acctgagtcg cctccagcat gcccttaatc
      541 gtacagtttt catttaccgt gacattgttg agcgtcccgg cgttcgcatt cacactgcca
      601 ctgatatccg catttttagc ggtcagcttt ccgtccggag tcagggaaaa tgccgatgga
      661 tttccaccgc tggtaatggt gggggccgtc aggcgtttca ggaacacgtc gttcatgaat
      721 atctgatcgc cctgaccaac aaacatcggt tttgtgttgc cattcgcagg attaaccatc
      781 gcaatcctgt ccgccgccag cagcacctga ctctgcatgc cgtcaggggt gttctcaata
      841 ccggcaccaa tacccgcgat ataaaggcgt ccgtcctgca tctgctgcag cttcacagcc
      901 cacatgctgt tcaggttatt atttgtatca acctgaacct tctgtatctg ctgaattgcc
      961 gcactctggt cttccagttt cttattgacg gtctgcgtga tttcattgct gacatccgta
     1021 atggacgtcc tgatttcagc caggtcaggc gcaagctgac cgttatcaat ctgcgtccac
     1081 agctcctgag ccagatgggt tttccctatc tcgcctttga aaaaatccag ataaccggat
     1141 gcatcatcac tcggctgacc aacagcctcc acgaatgccg atttgccaac ggtgttcaca
     1201 ctgcggatat aaaagtaata atcatggccc ggtttgatat tgatactggc ggctatccag
     1261 tacagcgccg tgccaagata gcgtgctgtg gtttcaacct gcctgatatc cgcaatccgc
     1321 ttttccgaga accagaactc aaactgtacc gtcggatcat aaaccgcaag atgcggcgtg
     1381 gcggttatct gaaaatagcc cggcgtcagc tcaatccgcg acagcgctgc cggtgcggca
     1441 atccggaacg ataccgacgc cggatcgccc tgctgccccc acgcatttac tgcccggact
     1501 gtcagcctgt agttccccag cgccagttgt gtgaagcggt aagtggtttc cgtcgtccgg
     1561 gccgtgctga ccagccgctc actgccgtca tccgctgtta cggtcagacg gagcag
//
LOCUS       DAZPBM010000340         1570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPBM010000340 DAZPBM010000000
VERSION     DAZPBM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.343"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            211..>1570
                     /gene="istA"
                     /locus_tag="ACHUZQ_004420"
     CDS             211..>1570
                     /gene="istA"
                     /locus_tag="ACHUZQ_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR1027921.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PER"
BASE COUNT          398 a          438 c          393 g          341 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgcctga ccttcctggg cggcgtaccg aagctgctca tccccgataa cctcagaagc
      841 gcagtgaaaa aagccgatcg ctatgagcct gtcatcaacg acagttatca ggcgctggct
      901 gagcactatg gcacggttat tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa
      961 gcagaaaacg gcgtcctcat cgtcgagcga tggcttcttg cccgcatccg taatgaaacc
     1021 ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga catgaataac
     1081 cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat gctggatgca
     1141 ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa agccgtgaag
     1201 gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc acacgaactg
     1261 gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg gcacaagggg
     1321 cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac caatccgctt
     1381 cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct tatcgagcag
     1441 ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa agccaaaccc
     1501 catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa aaaatacggc
     1561 ccggaacggc
//
LOCUS       DAZPBM010000341         1526 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPBM010000341 DAZPBM010000000
VERSION     DAZPBM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.344"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            568..1023
                     /gene="csgB"
                     /locus_tag="ACHUZQ_004421"
     CDS             568..1023
                     /gene="csgB"
                     /locus_tag="ACHUZQ_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1027922.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1064..1516
                     /gene="csgA"
                     /locus_tag="ACHUZQ_004422"
     CDS             1064..1516
                     /gene="csgA"
                     /locus_tag="ACHUZQ_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1027923.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          472 a          282 c          344 g          428 t
ORIGIN      
        1 taaacatgta actaaatgca actcgttata ttaaaatgtt aatcttaagg ttttattaag
       61 tttagaaatg atagaaaagt tgtacatttg gtttttattg cacaatttta aaaaatcata
      121 caaatggtga taacttacta ataatgcata taaaaaatat ttcggtgtag tcctttcgtc
      181 atgtaaaaag ttgttgtttt ttctccacac ctccgtggac aaatttttac tccaaaaaga
      241 cgaggtttgt cacggcttgt gcgcaagaca tatcgcagca atcagcgatg ggcaagaaga
      301 atgactgtct ggtgattttt gatagcggaa acggagattt aaaagaaaac aaaatatttt
      361 ttgcgtagat aacagcgtat ttacgtggct tttaatactt tggtatgaac aaaaaaagaa
      421 aaatacaacg cgcgggtgag ttattaaaaa tatttccgca gacatacttt ccatcgtaac
      481 gcagcgttaa caaaatacag gttgcgttaa caaccaagtt gaaatgattt aatttcttaa
      541 atgtacgacc aggtccaggg tgacaacatg aaaaacaaat tgttatttat gatgttaaca
      601 atactgggtg cgcctgggat tgcagccgca gcaggttatg atttagctaa ttcagaatat
      661 aacttcgcgg taaatgaatt gagtaagtct tcatttaatc aggcagccat aattggtcaa
      721 gctgggacta ataatagtac tcagttacgg cagggaggct caaaactttt ggcggttgtt
      781 gcgcaagaag gtagtagcaa ccgggcaaag attgaccaga caggagatta taaccttgca
      841 tatattgatc aggcgggcag tgccaacgat gccagtattt cgcaaggtgc ttatggtaat
      901 actgcgatga ttatccagaa aggttctggt aataaagcaa atattacaca gtatggtact
      961 caaaaaacgg caattgtagt gcagagacag tcgcaaatgg ctattcgcgt gacacaacgt
     1021 taatttccat tcgactttta aatcaatccg atgggggttt tacatgaaac ttttaaaagt
     1081 agcagcaatt gcagcaatcg tattctccgg tagcgctctg gcaggtgttg ttcctcagta
     1141 cggcggcggt aaccatggtg gtggcggtaa taatagcggc ccaaattctg agctgaacat
     1201 ttaccagtac ggtggcggta actctgcact tgctctgcaa actgatgccc gtaactctga
     1261 cttgactatt acccagcatg gcggcggtaa tggtgcagat gttggtcagg gctcggatga
     1321 cagctcaatc gatctgaccc aacgtggctt cggtaacagc gctactcttg atcagtggaa
     1381 cggcaaaaat tctgaaatga cggttaaaca gttcggtggt ggcaacggtg ctgcagttga
     1441 ccagactgca tctaactcct ccgtcaacgt gactcaggtt ggctttggta acaacgcgac
     1501 cgctcatcag tactaataca tcattt
//
LOCUS       DAZPBM010000342         1525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPBM010000342 DAZPBM010000000
VERSION     DAZPBM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.345"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            97..360
                     /locus_tag="ACHUZQ_004423"
                     /pseudo
     CDS             97..360
                     /locus_tag="ACHUZQ_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(397..954)
                     /locus_tag="ACHUZQ_004424"
     CDS             complement(397..954)
                     /locus_tag="ACHUZQ_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1027924.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="ACHUZQ_004425"
     CDS             complement(951..1226)
                     /locus_tag="ACHUZQ_004425"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR1027925.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="ACHUZQ_004426"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="ACHUZQ_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          325 c          367 g          409 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAZPBM010000343         1517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPBM010000343 DAZPBM010000000
VERSION     DAZPBM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.346"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            555..>1517
                     /gene="istA"
                     /locus_tag="ACHUZQ_004427"
     CDS             555..>1517
                     /gene="istA"
                     /locus_tag="ACHUZQ_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFR1027926.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
BASE COUNT          375 a          403 c          362 g          377 t
ORIGIN      
        1 tttatccctg ctttgttagc tccctaacct tgtacagaga gcctccataa gctcccatat
       61 caagcagaca tggttcgcag ttactgacat acaccagaaa actctgacag cagactcact
      121 tatcctccac ttcactcata tccttattgg tgagatgaac ctgaatgaca tgttttgcga
      181 cataaacgcc aaccagtgta tatttcacct gtagagattc tacaatctgt agcgttatgc
      241 atctaatgtt aggctctatg atgtcagaaa tctgcgagac tctacacctt ctgcatttac
      301 ctttatttat caccatgctc ctcagttaac ggagaacgtg ttcattgcat tttgtcactt
      361 acatttcgcc tttggtaccg gggggatata ggttccaacg ttccgcatga gaacctgaac
      421 cggcttcacg ccaccttgat accagatgcc gcacaggcag tcaacaagtg tcacgaacgg
      481 tgcaatagtg atccacaccc aacgcctgaa atcagatcca gggggtaatc tgctctcctg
      541 attcaggaga gcttatggtc acttttgaga cagttatgga aattaaaatc ctgcacaagc
      601 agggaatgag tagccgggcg attgccagag aactggggct ctcccgcaat accgttaaac
      661 gttatttgca ggcaaaatct gagccgccaa aatatacgcc gcgacctgct gttgcttcac
      721 tcctggatga ataccgggat tatattcgtc aacgcatcgc cgatgctcat ccttacaaaa
      781 tcccggcaac ggtaatcgct cgcgagatca gagaccaggg atatcgtggc ggaatgacca
      841 ttctcagggc attcattcgt tctctctcgg ttcctcagga gcaggagcct gccgttcggt
      901 tcgaaactga acccggacga cagatgcagg ttgactgggg cactatgcgt aatggtcgct
      961 caccgcttca cgtgttcgtt gctgttcccg gatacagccg aatgttgtac atcgaattca
     1021 ctgacaatat gcgttatgac acgctggaga cctgccatcg taatgcgttc cgcttctttg
     1081 gtggtgtgcc gcgcgaagtg ttgtatgaca atatgaaaac tgtggttctg caacgtgacg
     1141 catatcagac cggtcagcac cggttccatc cttcgctgtg gcagttcggc aaggagatgg
     1201 gcttctctcc ccgactgtgt cgccccttca gggcacagac taaaggtaag gtggaacgga
     1261 tggtgcagta cacccgtaac agtttttaca tcccactaat gactcgcctg cgcccgatgg
     1321 ggagcactgt cgatgttgaa acagccaacc gccacggtct gcgctggctg cacgatgtcg
     1381 ctaaccaacg aaagcatgaa acaatccagg cccgtccctg tgatcgctgg ctcgaagagc
     1441 agcagtccat gctggcactg cctccggaga aaaaagagta tgacgtgcat cctggtgaaa
     1501 atctggtgag cttcgac
//
LOCUS       DAZPBM010000344         1507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPBM010000344 DAZPBM010000000
VERSION     DAZPBM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.347"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHUZQ_004428"
     CDS             <1..207
                     /locus_tag="ACHUZQ_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027927.1"
                     /translation="DAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGV
                     EYAFPDPQTQNETQQAGYDENIPV"
     gene            258..446
                     /locus_tag="ACHUZQ_004429"
     CDS             258..446
                     /locus_tag="ACHUZQ_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1027928.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKTKLTKSECEKMLSGEKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            546..761
                     /gene="xisR"
                     /locus_tag="ACHUZQ_004430"
     CDS             546..761
                     /gene="xisR"
                     /locus_tag="ACHUZQ_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR1027929.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            763..>1507
                     /locus_tag="ACHUZQ_004431"
     CDS             763..>1507
                     /locus_tag="ACHUZQ_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR1027930.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          523 a          279 c          347 g          358 t
ORIGIN      
        1 gacgccaaac gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa
       61 agaatcaaag gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa
      121 ctggaaggcg ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca
      181 ggatacgatg agaatattcc ggtgtaaaac caagtgcatt ttggacacgg tttttgaaaa
      241 tattaatgga ggtgttcatg tataaaatta cagccactat tgaaaaggaa ggtggttctc
      301 ctgccagctg gacaagatac tcaaagacaa aactaacgaa atcagaatgc gaaaaaatgc
      361 tctcagggga aaaagaagca ggcgtttcca gagagcagaa agtaaaactg ataaatttta
      421 attgcgagaa acttcagtcc tcgtgaattg cattgtattc aaattaaaac ttcatagctg
      481 attattaata atcaacatcg ggcgtcaatt tcagcctaac attggcgcct gccagaggtg
      541 atgcgatggc acaagtaatc tttaatgaag agtggatggt tgaatacggc ctgatgcttc
      601 gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg gtggaaggct
      661 tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg attatctggt
      721 acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa attaccaaca
      781 ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg aaaacgatgt
      841 cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc cgggaattta
      901 agagcgttga tagttcatga aatcagttcc ggtgaatttg agtatttaag acgttttccc
      961 cagtccagca ctggggcaaa aatggtgaca acgagggtca taaaaacatt cggggagctt
     1021 tgtgatatct ggacaaaaat taaagagaca gagttaacaa caaacacaat gaagaaaacg
     1081 aaatcacaat taaaaacact caggataata atttgtgaga gtaccccgat atcgcatatt
     1141 cgttatagcg atatcttaaa ctaccggaat gaactgctgc atggagaaac gctttaccta
     1201 gataatccaa gatccaacaa aaaaggaaga accgtgcgca cagttgataa ctatatcgcc
     1261 ctgctctgtt cgttgttacg ttttgcgtat cagtcgggat ttatatcaac caaaccattt
     1321 gaaggagtaa aaaaattaca gcgaaacaga ataaagcctg acccgttatc taaaacagaa
     1381 ttcaatgcat taatggaaag tgaaaaagga cagagccaaa acttgtggaa atttgccgtt
     1441 tactccgggc ttcgtcacgg ggaactggca gctctggcgt gggaggatgt ggatttcgag
     1501 aagggaa
//
LOCUS       DAZPBM010000345         1482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPBM010000345 DAZPBM010000000
VERSION     DAZPBM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.348"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHUZQ_004432"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHUZQ_004432"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1027931.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHUZQ_004433"
                     /pseudo
     CDS             complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHUZQ_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          414 a          324 c          370 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcgaatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tc
//
LOCUS       DAZPBM010000346         1444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPBM010000346 DAZPBM010000000
VERSION     DAZPBM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.349"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1236
                     /locus_tag="ACHUZQ_004434"
     CDS             <1..1236
                     /locus_tag="ACHUZQ_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR1027932.1"
                     /translation="NVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLIL
                     SLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYG
                     KDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGIN
                     YTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYIS
                     RGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVK
                     DFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFT
                     ELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEAD
                     KALREAKSEGGNKVIIHHI"
BASE COUNT          391 a          305 c          282 g          466 t
ORIGIN      
        1 aatgtaaaac tcatcccaca gatgaattac ctgatggttg ttgtggcttt gtttttcctt
       61 aacgccgtca tttttctttt catgttaatg aaatatttca ctaacaaaca aattttacca
      121 acactcattt taagccttgc atttttaagt ggccttatct atttagttga aaccattgta
      181 attatccata aaccaattaa cggcagtaca ctgatccaga caaagtcgaa tgatgtttct
      241 attttctata ttttccgcca actcagtttt atttgtttaa cctcgctggc actcttttgt
      301 tatggaaaag acaacatcct tgacaacaat aagaaaaaaa cgggaatcct gttgctggcg
      361 ctgattcctt ttttagtttt tccccttctg gcacacaatc tgagcagtta taacgctgac
      421 tattctttgt atgtcgtcga ctactgtccg gacaaccata ctgcgacctg gggaatcaac
      481 tatacaaaaa tattggtttg tctgtgggca tttttactgt tctttattat catgcgtaca
      541 cgattagcca gcgaactatg gccgttaata gcattattat gtctggcatc gctatgctgc
      601 aacttacttc tactgactct ggatgagtat aattacacca tctggtatat cagtcgcggg
      661 attgaagttt ccagtaaact gtttgttgtg tcttttctga tttataacat ttttcagaaa
      721 ctgcaactct ccagcaaact ggcagttcat gatgtgctga ccaatattta taatcggcgc
      781 tactttttca acagcgtaga gtcattattg tcgagacctg ttgttaagga cttctgtgtc
      841 atgctggttg atattaatca gttcaaacgc atcaatgccc aatggggaca tcgtgtgggt
      901 gataaagtgc tggtttcaat tgtcgatatt atccagcaaa gcatccgccc cgatgatatt
      961 ttagcgcgac tggagggtga ggtgtttggc ttgctattta ccgaactcaa tagtgcccag
     1021 gcaaaaatca ttgcggaacg tatgcgtaaa aatgtcgaac tcctgaccgg ctttagtaac
     1081 agatatgatg ttcctgaaca aatgaccatc agtattggca cggttttttc aacgggtgac
     1141 acgcgtaata tctcgcttgt catgacggaa gcagataaag ccttacgcga agcgaaaagc
     1201 gaggggggca acaaagtgat tattcatcat atttaagtgc aaaaatattc agagccatgc
     1261 tttttgcgtg gcttttgcat acaatttatt actattaccc ctaaatttcc cctccccagc
     1321 ggcgcagaga tgagtataat tagcgcccct gtgccaggcc gcaatcgaac tttatctggt
     1381 tttctcgttt cactaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc
     1441 cggc
//
LOCUS       DAZPBM010000347         1437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPBM010000347 DAZPBM010000000
VERSION     DAZPBM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.350"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHUZQ_004435"
     CDS             <1..1117
                     /locus_tag="ACHUZQ_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1027933.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..1334
                     /locus_tag="ACHUZQ_004436"
     CDS             1095..1334
                     /locus_tag="ACHUZQ_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027934.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGAPIKLSSML"
     gene            complement(1329..>1437)
                     /locus_tag="ACHUZQ_004437"
     CDS             complement(1329..>1437)
                     /locus_tag="ACHUZQ_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027935.1"
                     /translation="QGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          385 a          300 c          439 g          313 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaaga gggtgcccct attaagttgt
     1321 caagcatgtt atgacccctg cggggtataa aaagtcccgt cgcgcatcat ggcgaacaga
     1381 acgtcgcagc gtcgtctcgc cagggcgata agcgcctgat tgtgtcgttt tccctga
//
LOCUS       DAZPBM010000348         1427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPBM010000348 DAZPBM010000000
VERSION     DAZPBM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.351"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..119)
                     /gene="rutC"
                     /locus_tag="ACHUZQ_004438"
     CDS             complement(<1..119)
                     /gene="rutC"
                     /locus_tag="ACHUZQ_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1027936.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(131..823)
                     /gene="rutB"
                     /locus_tag="ACHUZQ_004439"
     CDS             complement(131..823)
                     /gene="rutB"
                     /locus_tag="ACHUZQ_004439"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1027937.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(823..>1427)
                     /locus_tag="ACHUZQ_004440"
     CDS             complement(823..>1427)
                     /locus_tag="ACHUZQ_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1027938.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          311 t
ORIGIN      
        1 ttatgttgat caaaagccag cgtaccggag acatacacca cgccatcagc cagcgtgccg
       61 ggaacgaagg gggccagcgg tgcgctgctg ccagcgggaa taattacgga ttttggcatc
      121 gttaaactcc ttaagcgata cgagcaaagg acgtgggaga aagcgcgtcg cagaacgttt
      181 cgacgtcgct gacccagcca aaaaaggttt cgatattgaa caacgcggct ttctgtgcaa
      241 attcaggccc cgcctggtgt gttgcgtctt ccagcaccac gccgaaatac tccagaaaaa
      301 agccatcgcg tagcgtcgat tcgacgcaga cgttggtagc gataccggtg aaaaccagat
      361 ggcgtattcc acggctgcgc aaaatgctgt ccagcggcgt attgaagaaa ccgctgtagc
      421 gcggcttcgg cagcacaata tcgccaggct gcggcaccag ttcatccacc agttgataat
      481 cccaggagcc tttcgccagc aatttcccct gcagctgcgg ctgcttacgc atggttttca
      541 gggcgttcga tttatgaaaa ttcggtgagc cgggtccgcc agcttcgaca tactgttcat
      601 cccagccatt ttgaaaccag atgatcagca tccctgccgc tcgcgctgcg gtcacggcgg
      661 tttgaatgtt ggcaatgacc gggcgagtgg ttgagacatc aaacccggcg agatctaagt
      721 agccgcctgg cgtggcataa gcgttttgca tatccaccac gatcagcgca ctttgctgcg
      781 gatcgaaggt aatggcttcc ggtcgagcgg ttaaggtcgt catcatgcca cctcctgagt
      841 cagcgcaggg agatgggcgc ggcactgcat cagtggttga atgcgctcgc cgaaggtttc
      901 gattcccgac agaaaatcat cgaaggtcaa cagcacgcct tcggcaccag gcacgcttgc
      961 gacttcatct aacatgcgcg cgacactggc gtaagaaccg actaatgtcc ccatattgat
     1021 gtttaccgcc gaagtgggat cggccatctg acggacgttg gtgtcagtac cggagcgggt
     1081 atctttctga ctttgttcgg ttagccagct taacgcctct tcatccgcgc ccgctttgta
     1141 gtgttcccat ttggcgcgag cggcatcgtc ggtttcatcg gcaatcacca taaacaacac
     1201 ataagagcca acgtcgcgcc cggtttgctc tgcggcctgt ttcatccgca cagcggtcgg
     1261 ggcgaaagcc gtgggtgtat ttacgccttt gccgaaacag aagttgaaat cggcatactg
     1321 agcggagaac gccatgccag cgtcgctttg cccggcgcag atcactttca tggggacact
     1381 cggttgcgga ctgacgcgac aatcattcat ggtgaaaaaa tcgcctt
//
LOCUS       DAZPBM010000349         1422 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPBM010000349 DAZPBM010000000
VERSION     DAZPBM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.352"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(319..>1422)
                     /locus_tag="ACHUZQ_004441"
     CDS             complement(319..>1422)
                     /locus_tag="ACHUZQ_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1027939.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          372 a          382 c          325 g          343 t
ORIGIN      
        1 tagattaaat attttttaaa tgtgactgtc atccttcaga taataaagcc atcatcgcaa
       61 tagatggaaa agcactcctg ctcttttttg attagagccg tcgcaggagg aacaactcat
      121 atcatcattg cgatctcgac gatgtatagt ctcatcctgc ggcagaacaa gacggataaa
      181 aaatccaatg agagcacact catccagaac ttattaacat aatgaatatt aaagaaaaat
      241 catcgcagcc aatgaagtaa tatgcaaaaa taattagccc ttgcagggag caaagttcca
      301 taatctcccc cttccccctc aatgatccaa atacaaataa tgcatccaac tggtcattct
      361 tagcagcacc ttccgcatca ccgaaacatg gctgaaatgg tcggagtaaa ctgctacctg
      421 ggcgtaaatg ccgtcgggta aggcatcgat atcatcgtta gggtccagtt caatggttcc
      481 cagcacgccg tccgtgccgg gaacgaccgt taatgattgc aataccccct gcgcctgata
      541 agaaccgcct ggcacgacag gtaaaatact agtcaatttg ccgtggaaca cctgcccagg
      601 tagcgcgtta aacaccactt ccgcatcatc gccaggtttc agacgtaaca gcgagttttg
      661 ccgaaattgg gcgacaattt gccgtttttg ctcggggatg aagaccatca ccggacgcag
      721 cggcaaggca gctgcgtatg tacctgggcg gatcagtacc tgagtaacgt agccattgct
      781 cggcgcgcga atgacagtct gctcaaggtt atattttgct tcagtaagtt gcgctcttaa
      841 gctcacaatc tgcgattgct cgccgttaac catactgtcg agctggctct ggatctgcgc
      901 ctgctccgcc accgagcctt tcaccagcgc atcctgcgcg aggaaatttt gccgcgcatc
      961 gtcgatgtca cgttccgaga acggattcac cgccgcctgg ctgcctttca ggtaacgttg
     1021 ataattttta aacagacggt cgcgctccgc tgaaacctgg gtggtgttgg cctgcgcttc
     1081 agtgagctgc gcacgcagcg tctttatatt atgcgtcgcc gtcatcaggt cagcctgaag
     1141 tctgtcaact cgcgcctggt aacgaaccgg gtcgagctta aaaagcacct cgcccttttg
     1201 aataagctga ttattcttgt cagtgacttc agtaacaatt cccgtcacct gtggcgtgat
     1261 agggatcgct atcactgcct tttgcgcggt aaaagtgtaa gggtggttgt agttcatcaa
     1321 caaaatcaaa ccactcacca gaaacacgcc tcccagcgcc gccgtcgcca gcgtccactg
     1381 attcaccgga atacgaaaga ttttaaagac cgcccacgcc ag
//
LOCUS       DAZPBM010000350         1421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPBM010000350 DAZPBM010000000
VERSION     DAZPBM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.353"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHUZQ_004442"
     CDS             <1..>1421
                     /gene="rhsC"
                     /locus_tag="ACHUZQ_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR1027940.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          422 c          436 g          225 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAZPBM010000351         1402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPBM010000351 DAZPBM010000000
VERSION     DAZPBM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.354"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..440
                     /gene="rfaY"
                     /locus_tag="ACHUZQ_004443"
     CDS             <1..440
                     /gene="rfaY"
                     /locus_tag="ACHUZQ_004443"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR1027941.1"
                     /translation="IVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVE
                     LNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAER
                     KARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
     gene            466..>1402
                     /locus_tag="ACHUZQ_004444"
     CDS             466..>1402
                     /locus_tag="ACHUZQ_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1027942.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLHLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQ"
BASE COUNT          422 a          248 c          300 g          432 t
ORIGIN      
        1 tgatcgttga aaccgatcgt gtcagaagtg caggtcttac ttttcctaat gatttttatt
       61 ttctggctga gcgtaaaatt ttcaactatg ccagtgtttt tatcatgctt atcgagtatg
      121 tcgagggcgt agagctcaat gatatgccga tcattccaga agatgttaaa gcagaaatta
      181 aggcttcaat ggaaaaattg catgccttaa atatgctatc gggcgaccca caccggggta
      241 actttatcgt tagtaaagat ggtgtcagga taattgacct atccggtaaa agctgtacgg
      301 cagagcgtaa agctcgtgat cgtctggcca tggaacgtca cctgggtatt gccaacgaaa
      361 taaaagatta tggctactat tcagtaatct acagaaccaa actacgcaaa tttattaaaa
      421 aattaaaagg caaagcgtaa accacacagt caaaacggaa ccaacatgga tttattagct
      481 gagagtatta ctgaagtcgc tgtctctggg gaaattgcta acaccgatcg tgtgttaaat
      541 atcgcttacg gtattgaccg taacttttta tttggtgcgg cagtatctat gcaatcagtt
      601 gttatgcata atccggacct tgcggttaag tttcatctct ttaccgacta cattgatgaa
      661 gattatctac aacgtgttaa tgcttttacc agcaaaaatg ctaacgttga agtaagaatt
      721 tataaagtct ccagtgcctt tattgatatc ttccccagcc tgaaacagtg gtcttatgca
      781 acattcttcc gtttagttgc gttccagtat ctgagtgaaa ctattgaaaa tctgttatat
      841 atcgatgctg atgtcatctg taaaggctca ttagctggat tgcttgatat taattttgat
      901 ggggataagt ttgcagctgt tattaaagat gtgcctttta tgcaggaaaa accagcgaag
      961 cgtctggcta tagagggact tccagggaat tatttcaacg ccggtgtagt atatctgcat
     1021 cttgaagcat gggcgaaaaa tgattttatg aataaagcca ttgctatgct ggcaagtgac
     1081 ccgcagcaca cgaagtataa atgccttgat caggatattt taaatattct gttctttggt
     1141 cattgtattt ttattagcgg cgattatgat tgcttttatg gcatcgatta tgagttaaaa
     1201 aataaaagcg atgaagatta taaaaagacc attaccgatg atactaagct gattcattat
     1261 gttggcgtaa cgaagccctg gaacgactgg acgaattatc cctgccagaa gtattttaat
     1321 gaggcttatc aggcttcttg ctggaatgat gtggcgttta ttccagccac gaatgaaaag
     1381 cagtatcaag tgaaatatca ac
//
LOCUS       DAZPBM010000352         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPBM010000352 DAZPBM010000000
VERSION     DAZPBM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.355"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUZQ_004445"
     CDS             <1..459
                     /locus_tag="ACHUZQ_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1027943.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHUZQ_004446"
     CDS             477..1115
                     /locus_tag="ACHUZQ_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1027944.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZPBM010000353         1384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPBM010000353 DAZPBM010000000
VERSION     DAZPBM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.356"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHUZQ_004447"
     CDS             complement(<1..1339)
                     /locus_tag="ACHUZQ_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1027945.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZPBM010000354         1378 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPBM010000354 DAZPBM010000000
VERSION     DAZPBM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.357"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..985
                     /locus_tag="ACHUZQ_004448"
     CDS             <1..985
                     /locus_tag="ACHUZQ_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1027946.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            1000..>1378
                     /locus_tag="ACHUZQ_004449"
     CDS             1000..>1378
                     /locus_tag="ACHUZQ_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1027947.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          316 c          362 g          340 t
ORIGIN      
        1 cttagtggtg ggtgttccgg ttccggacaa tattcacccg atgatcaaag acgaagcagg
       61 agtctggtcg tggcgcacgc ctgtcctgaa aggcaacctg tatgagtatt ttttcaatgt
      121 tgatggtgta cgcagcattg atataggcac cgcaatgact aagcctcagc gccaggttaa
      181 ctccagtatg gttctggtgc caggcagtta tctggatacg cgttctgttg cgcatggtga
      241 tttgatcgcc ataacttacc actccaacgc attgcaatct gaacgtcaga tgtatgtctg
      301 gaccccgcca ggatacaccg gcatgggcga gcctttgcca gtgctctatt tctatcacag
      361 ttttggtgat accggacgtt ccgctatcga tcaggggcgt atcccgcaaa tcatggataa
      421 cctgctggct gaagggaaaa ttaaaccgat gctggtggtg atcccggata ccgaaaccga
      481 tgcgaagggc attattcccg aagatttcgt gcctcaggaa agacgtaaag tcttttatcc
      541 gctgaatgct aaagcggcag atcgcgaact gatgaacgat attatcccgc tgattagcaa
      601 gcgttttaat gtccgtaaag atgccgatgg tcgcgcgctg gcagggcttt cacaaggcgg
      661 gtaccaggcg ctggtttccg gaatgaatca tctggaaagc tttggctggc tggccacatt
      721 cagtggtgtt accacgacaa ccgtaccgga tgaaggtgtc gcggcccggc tgaacgatcc
      781 ggcagctatt aaccagcaac tacgtaattt tactgtggtc gttggtgata aagatgtcgt
      841 aaccggcaag gatatcgccg ggctgaaaac tgagcttgag cagaaaaaaa ttaagtttga
      901 ttaccaggaa tatcccggcc tgaaccatga aatggatgtc tggcggcctg cctatgcagc
      961 cttcgtacag aaattattta aataaaataa ggcattaaga tgaaattaat cattaccgaa
     1021 gattaccagg aaatgagccg tgtcgcggca caccatctgc ttggttatat gtcgaagacg
     1081 cgtcgtgtta acctggcaat taccgccggt agcacgccca aaggcatgta tgaatacctc
     1141 accaccctgg ttaaaggtaa gccctggtac gataactgct atttctataa tttcgatgaa
     1201 attccctttc gcggcaaaga gggagaaggc gtaacgatta ccaatctgcg taatctgttt
     1261 ttcacccctg cggggatcaa agaagagaat atccagaagc tcactattga taactaccgc
     1321 gagcatgatc agaaactggc gcgtgaaggc ggcctggatt tggtggtgct gggattag
//
LOCUS       DAZPBM010000355         1365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPBM010000355 DAZPBM010000000
VERSION     DAZPBM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.358"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1261)
                     /locus_tag="ACHUZQ_004450"
     CDS             complement(<1..1261)
                     /locus_tag="ACHUZQ_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1027948.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          380 a          348 c          305 g          332 t
ORIGIN      
        1 aggtgtaacc tattgcgttg tttgtattac ggtattcggc aacgacttta atcattccct
       61 ccatcatact tgccacttct gtttcctgcg gcgagatcat gcggacattt ttcatgacct
      121 gggattgcat caccgtctgg ctaccagagt cttccgggcg ttgccaggtc tggatctcct
      181 gatcgttacc gccaacagta cgccagttgg taattgcacc actgaagatg tcacgcacct
      241 gttgttcagt cagggaatta accggattat ccgcattgac gatgaaaaca aacgcttcac
      301 gggcaaaagg cgtgtacagc aaagtaacgc ccgattcctc agcgcgtttt ttctgcccac
      361 cggaaggttg cgccacaaaa ataatatcag catcaccttt aacaatcctg ttatacgcat
      421 cgggggtacg agagctctcc agatattccc tggtgtgaaa atcttccggt ataacgctta
      481 aagcataaaa tgcagaggca taaattgggt acgccgccgt agcaccatcg agtcgcggcc
      541 agttttgcgt aaactgaatt tgcggtttac cacgcagtgg tgtcagttga ttattgagtt
      601 tatcgggtcg ccaggcccag gtatcaatat tttcgcgcac accgacaccg ggattgagct
      661 gtttatataa ataaccctga taaccagcga caaaaccaag catgagaagg ataaacagga
      721 gggaattacg gcaacgtagc ccggatgtac cggttaaagg atgtctggtt ggccggccgt
      781 accccagcga aaaactgata tgcgcgccaa cgggtataat gactatccac cagtactcac
      841 ctccgactac tgagactaac aaaacgccca gccaggggag cataagatca gccaaccaac
      901 ccgatcccat ctggtgaaat tgataatctg ccagatgcag cgcgattatc cagccaacca
      961 ggattaataa accgggtact aaaaaaagac attgtcgcca gacggctatt tctctttttt
     1021 ctggtgcact atcgcctgcc catactcgcc ccgagaaaaa tagcattaat gcgcccagcg
     1081 ccagcataaa aatgttgagc agaaggtaac ttgttggcgt gataaggata ttacaaactt
     1141 caataatacc ggcaagtccg acacccagca tagcaacaac cgccgccaaa aattgcgtca
     1201 gtatggggat gccgaaaaaa gtcattacca gcgaggacaa aatccatttt ctgttttgca
     1261 ttattctttc cattcttttt gaatggtgaa attatactcc ccgagtcccc ttgccccttc
     1321 tggacacttt tccgaaatga tggcggaaaa aaacgggacc ctttg
//
LOCUS       DAZPBM010000356         1346 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPBM010000356 DAZPBM010000000
VERSION     DAZPBM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.359"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHUZQ_004451"
     CDS             <1..189
                     /locus_tag="ACHUZQ_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027949.1"
                     /translation="SATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQL
                     AQGKRRCQQQFTSQEVQN"
     gene            199..>1346
                     /gene="yicI"
                     /locus_tag="ACHUZQ_004452"
     CDS             199..>1346
                     /gene="yicI"
                     /locus_tag="ACHUZQ_004452"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1027950.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          336 a          320 c          353 g          337 t
ORIGIN      
        1 agcgccacga ttagcatcat tattgcgcta ttcacgattg ttccggcgat ctgttatttg
       61 ctgagcgcga ttatcgctaa acgctactac tcactcacga cgcacaatct gaaaaccgtt
      121 atggaacagc tggctcaggg taaacgccgt tgccagcaac aattcacctc tcaagaagtg
      181 cagaactaag gaacggcaat gaaaattagc gatggaaact ggttgattca acctggcctc
      241 aatttgattc acccgcttca ggtgttcgag gttgaacagc aggataatga aatggtggtc
      301 tatgctgccc cccgtgatgt gcgtgaacgt acctggcagc ttgatacgcc tttatttacg
      361 ctgcgctttt tctccccaca ggaaggtatt gtcggtgtgc ggattgagca ttttcagggg
      421 gcgctgaata acggtcctca ttatccgctc aatattttgc aggacgtgaa ggtcacaatc
      481 gaaaacacag aacgttatgc tgagtttaaa agtggcaact taagcgcgcg tgtcagcaaa
      541 ggtgagttct ggtcactgga ttttctgcgc aacggcgaac gtattaccgg tagtcaggtg
      601 aaaaataatg gctacgtgca ggacacgaat aatcaacgca attatatgtt tgagcggctt
      661 gatcttggcg ttggcgaaac agtttacggt ctgggagagc gctttactgc cctggtgcgc
      721 aatggccaga cggtagagac ctggaaccgg gacggcggca caagtactga acaggcgtat
      781 aaaaatatcc cgttctacat gactaaccgt ggttatgggg tactggtcaa tcatccccag
      841 tgtgtctctt ttgaagtggg atcggagaaa gtctccaaag tgcagttcag cgttgagagt
      901 gaatatctcg aatactttgt tatcgacggc ccgacgccga aagcggtact tgatcgttat
      961 acccgcttta ctggtcgtcc ggcgctgccg cccgcgtggt ccttcggcct gtggctaacc
     1021 acttcattta ccaccaacta cgacgaagcg acggtaaaca gctttatcga tggtatggcg
     1081 gaacgcaatc tgccgctgca tgttttccac tttgactgtt tctggatgaa agccttccag
     1141 tggtgcgatt ttgagtggga cccgctgact ttccctgacc cggaagggat gatccgccgc
     1201 ctgaaagcga aagggctgaa aatctgcgtc tggattaacc cctatatcgg tcaaaaatcc
     1261 cccgtcttta aagagttaca agagaaaggc tatttactca aacgcccgga cggttcgcta
     1321 tggcagtggg ataaatggca gccagg
//
LOCUS       DAZPBM010000357         1320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPBM010000357 DAZPBM010000000
VERSION     DAZPBM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.360"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHUZQ_004453"
     CDS             complement(<1..346)
                     /locus_tag="ACHUZQ_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027951.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERA"
     gene            complement(349..1059)
                     /locus_tag="ACHUZQ_004454"
     CDS             complement(349..1059)
                     /locus_tag="ACHUZQ_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027952.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1075..>1320)
                     /locus_tag="ACHUZQ_004455"
     CDS             complement(1075..>1320)
                     /locus_tag="ACHUZQ_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1027953.1"
                     /translation="FRYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVT
                     RENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          351 a          317 c          293 g          359 t
ORIGIN      
        1 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
       61 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      121 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      181 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      241 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
      301 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
      361 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
      421 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
      481 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
      541 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
      601 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
      661 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
      721 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
      781 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
      841 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
      901 accgacgaac ttttcccctt catccaaggc ctggttaata agttgctgaa caatgccttc
      961 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1021 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1081 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1141 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1201 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1261 gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa
//
LOCUS       DAZPBM010000358         1283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPBM010000358 DAZPBM010000000
VERSION     DAZPBM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.361"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            252..770
                     /locus_tag="ACHUZQ_004456"
     CDS             252..770
                     /locus_tag="ACHUZQ_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR1027954.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            980..>1283
                     /locus_tag="ACHUZQ_004457"
     CDS             980..>1283
                     /locus_tag="ACHUZQ_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1027955.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          316 a          313 c          330 g          324 t
ORIGIN      
        1 tttatggagg caatacggac tttgaacagt tgggtatggt tatccagata tatattatta
       61 agttattgct gaataagaga tttaatgatt ttaaaaatag tttttacgac aaccgtaaat
      121 tctagctcgg tttgtatgta aaaaatactg aaaaatgatg cggttccgtg aagtgagctg
      181 catctgcctg tctgggatta acggcaggtc attgtaagta gtaattaacc acgttaacaa
      241 ggagtttaca aatgccaaca ccgtgttata tctccatcac tggtcagacc cagggaaata
      301 ttactgcagg tgcatttacc gctgattctg tcggcaacat ctatgtgcag gggcacgaag
      361 acgagatgct ggtgcaggaa ttcctgcata acgttactgt cccgaccact cctcagtccg
      421 gtcagcctgc gggtcagcgt gctcacaagc ctttcatttt taccgtggcg ctgaacaaag
      481 cggttccgct gatgtacaac gcactggcct ccggcgaaat gctgccaacc actgaacttc
      541 actggtggcg tacctctgtg gagggcaaac aggagcatta tttcactacc cgcctgactg
      601 attcgacgat tgtcgatatg aaactgcata tgccgcattg ccaggacccg gcgaaacgcg
      661 agttcaccca gttgctcgag gtttcactgg cctaccgcaa aattgaatgg gaacacgtca
      721 aatctggcac ctccggtgcc gacgactggc gcgcaccact ggaagcataa gttaagccaa
      781 cagcatccgg ctatcaggcc ggatgctgtt tttgtattac gccgtagaaa gaaaggttgt
      841 cagtaagggg ttacgttcac gggagatggc ggatgccgga aacttcggcg gtagcgtgcc
      901 tgaacgcaag cgtaacactg tactgtcaca gccgctttaa cagcgaactg tctgtttttc
      961 cttaaccccg gaggttgcta tgtcaaccgg attacgtttc acgctggaag tggacggcct
     1021 gccaccggat gcttttgcgg tggtctcctt tcatctgaac cagtcactct cttcgctctt
     1081 ttccctcgag ctctctctgg tcagccagca gtttctctcc cttgaatttc cgcaggtact
     1141 ggacaaaatg gcctacctga cgatatggca gggcgatgac gtacagcgcc gggtgaaagg
     1201 tgtggtgacc tggtttgaac tgggggagaa cgacaaaaac cagatgctgt acagcatgaa
     1261 ggtatgccca ccgctgtggc gca
//
LOCUS       DAZPBM010000359         1272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPBM010000359 DAZPBM010000000
VERSION     DAZPBM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.362"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..476
                     /gene="espX1"
                     /locus_tag="ACHUZQ_004458"
     CDS             <1..476
                     /gene="espX1"
                     /locus_tag="ACHUZQ_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR1027956.1"
                     /translation="KSNLSYGYYFCCVLTNISCFESDLSNTIFSNGEINNLFIKKSNI
                     FGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIP
                     LVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSKMRKYLQSINECMLLIKNIVHQD"
     gene            710..1272
                     /locus_tag="ACHUZQ_004459"
                     /pseudo
     CDS             710..1272
                     /locus_tag="ACHUZQ_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          456 a          232 c          213 g          371 t
ORIGIN      
        1 aaaagagcaa cctttcatat gggtattatt tttgttgtgt attaaccaat atatcatgtt
       61 ttgaaagcga cttatcaaac accatctttt caaatggaga aataaacaac ctcttcatta
      121 aaaaaagcaa catatttgga acatcgttca ctaataccat gataaaaaat ttgcgctgtg
      181 aagatattat gcctggccga tggaccactc aacttgtcaa taagcactta ggttacagat
      241 acacaggagt attcaaaacc ctggcaagta ttgatgacaa accgtcccgt ttcgaaattt
      301 tgataccatt ggtacaaact ttagttcgcg ataatgtaaa gctaaataat gatgtgtata
      361 aagaacttaa taagtttatg cacgattacg acaaaacatc atcaaaaatg agaaaatacc
      421 ttcagagcat caacgagtgc atgctcctta taaaaaatat agttcatcag gattaaaaca
      481 ctcaaagaaa gaggtaattt tagaatgaaa ttatctgtga tgattattaa tacactgcag
      541 agaaataaaa aacaatctga agcatgctct gaaacatcct actctgtcac tctaatgatg
      601 gcaccttgct cttcttataa agaagggaac aattaagaat attaaatttt caaactttca
      661 tcaacttcac atcaagaaat taaaaaattt aaacattaat ctaaataata acaacctgat
      721 cacataatcg ggtacgattt tatccgataa ctataatgct ataactctgg gaaatggttg
      781 gaatacaccg ctgggtgcca tctcttttta cgctgcccga tcgagtagta agttaaataa
      841 tgataccaga cacgaaggta cgagctatca ggtcgcctat aataaatatc tagtacaaac
      901 agccactcga tttagcgtgg ccgcctggcg ttatgcttcg caggattaca ggacattcag
      961 cgaccatctt tacgaaaatg ataaacacta tcatcagagt gattatgatg atttttatga
     1021 tattggcaga aaaaatagcc tttctgccaa tattatgcaa cctttatcca ataatctggg
     1081 aaatgtatca ttaagtgcgc tttggcggaa ttactggggg cgaagtggaa atgctaaaga
     1141 ttaccaattc agttactcca ataactggca acacattagt tatactttct ctgccagcca
     1201 atcttatgat gaaaataata aagaagagga gcgttttaat ctgtttatct ccattccttt
     1261 ctactggggg ga
//
LOCUS       DAZPBM010000360         1266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPBM010000360 DAZPBM010000000
VERSION     DAZPBM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.363"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(81..1025)
                     /gene="leuO"
                     /locus_tag="ACHUZQ_004460"
     CDS             complement(81..1025)
                     /gene="leuO"
                     /locus_tag="ACHUZQ_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1027957.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          389 a          288 c          251 g          338 t
ORIGIN      
        1 cacattcaga ataaaattct acattcaata aaaaatacag aataaaccag acattcatgt
       61 ctgacctatt ctgcattcag ttagcgtttg caaattgaga ctaattgctc ttccatccac
      121 tgatggcctt tatcgcgccc ggcagcttca tgccaggaga gataacaggt tctgctattt
      181 tgttttaacg gcagcggtaa tacctgtaat tctaaggatt cagcgaactc ttcagccagc
      241 caacgcggcg caatagcgac caaatgcgtt tgcgacacca cgctaagtac gctcatcatt
      301 gccatgccct gatacgcgat actggcttgc ttatctaccg tgtcatacca aggttgacta
      361 aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt cgttataaac atcatgtttc
      421 agtaacgggc ccttaattgt tggatgattt ttgctggcta ccagcaccat ttcatcttta
      481 aataatggca cgctggtaaa ttcaggacga tggaagtctt cataactaat cacaaactcc
      541 gtttcctgat aacgcagctg atgttcagtg ttctgattta atgaagactt gaacataaca
      601 tgtatatttg gcgcaatctg ctcaatgtga ttataaatct gcgaggtcag aatgctgtct
      661 aacgggctgc aaacacaaag atgaaataca cgttcactgc tcgcgggttc aaaacctgaa
      721 ccaggcaatt cattttgtac tagttgcaat gcctgacgaa ctgagccaaa aagttgaaat
      781 gcgcgagcag tcggttgaat accacggcca taacgaacaa aaagctcgtc attaaacatc
      841 accttcaggc gtgcaacagc gttactgacc gcaggttgcg acatacccag aacatgagcg
      901 gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa cggttaataa gttgagatcg
      961 accatgcgta gctgtggctt gcttaactcc gccgtctctg aatgatctgt ttgtacctct
     1021 ggcatactta actccactgt cacacttaac tccctttccc ttattggaat gcagagaaat
     1081 aatcctgaag aatatgattt atcatgcata taaaataaga aaaagcaaaa tgagtaaaat
     1141 tcggaaaaca taaagatacg ggttctgata aaaacagaat ccattgccgt ggaatgagtc
     1201 atttaccaca gcataagaat ccataatgtt tttgttgcga aaacaatcta atcataacta
     1261 ccgcga
//
LOCUS       DAZPBM010000361         1252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPBM010000361 DAZPBM010000000
VERSION     DAZPBM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.364"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..834
                     /locus_tag="ACHUZQ_004461"
     CDS             <1..834
                     /locus_tag="ACHUZQ_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1027958.1"
                     /translation="SRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLADPAYRR
                     RRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAANNITQSG
                     VTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKLDTRRGS
                     HSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGNTQARGL
                     RTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDEFVSVQL
                     A"
     gene            876..>1252
                     /locus_tag="ACHUZQ_004462"
     CDS             876..>1252
                     /locus_tag="ACHUZQ_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1027959.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTER"
BASE COUNT          286 a          330 c          386 g          250 t
ORIGIN      
        1 tcccgtggcg gctccacctc tgaatttacg ccgggatatg tcaaacccaa gcatgaggtg
       61 aatccgcaga tgaccctgcg tcgcctgccg gatgaagatc cgcagaatct ggcggacccg
      121 gcttaccgcc gccgtcgcat catcatgcag aacatgcgtg acgaagagct ggccattgct
      181 caggtcgaag agatgcaggc agtttctgcc gtgctcaagg gcaaatacac catgaccggt
      241 gaagccttcg atccggttga ggtggatatg ggccgcagtg cggcgaacaa catcacacag
      301 tccggcgtca cggagtggag caagcgtgac aagtccacgt atgacccgac cgacgatatc
      361 gaagcctacg cgctgaacgc cagcggtgtg gtgaatatca tcgtgtttga cccgaaaggc
      421 tgggcgctgt tccgttcctt caaagccgtc aaggagaagc tggatacccg tcgcggctct
      481 cattccgagc tggagacagc ggtaaaagac ctgggcaaag cggtgtccta taagggaatg
      541 tatggcgatg tggccatcgt cgtgtattcc ggacagtacg tggaaaacgg cgtcaaaaag
      601 aacttcctgc cggacaacac gatggtgctg gggaacactc aggctcgcgg tctgcgcacc
      661 tatggctgca ttcaggatgc ggacgcacag cgcgaaggca ttaacgcctc tgcccgttac
      721 ccgaaaaact gggtgaccac cggcgatccg gcgcgtgagt tcaccatgat tcagtcagca
      781 ccgctgatgc tgctggctga tcctgatgag ttcgtgtccg ttcaactggc gtaatcatgg
      841 cccttcgggg ccattttctc tctgtggagg agttcatgac gaaagatgaa ctgattgccc
      901 gtcttcagga gctgggtgag caattgaacc gcgatgtcag tttgacggga acgaaagaag
      961 aactggcgct tcgtgtggca gagctggaag aagagcttga tgacacggat gaaactgccg
     1021 gtcaggacac ccctctcagc cgggaaaatg tgctgaccgg gcatgaaaat gaggtggtat
     1081 cagcgcagcc ggataccgtg attcaggata cggctgaact ggtcacggtc gtggcactgg
     1141 tgacgctgca tactgatgca cttcacgcca cgcgggataa acctgtggca tttgtgctgc
     1201 cgggaacggc gtttcgtgtc tctgccggtg tggcagccga aatgacagag cg
//
LOCUS       DAZPBM010000362         1249 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPBM010000362 DAZPBM010000000
VERSION     DAZPBM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.365"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..1249
                     /gene="rhsB"
                     /locus_tag="ACHUZQ_004463"
                     /pseudo
     CDS             1..1249
                     /gene="rhsB"
                     /locus_tag="ACHUZQ_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          304 a          362 c          384 g          199 t
ORIGIN      
        1 agaatttacc tgccgcgccg ctggtgcgct atggctggac gccacgcggc gaactggcgg
       61 tggtgtatga ccgtagtggc aaacaggtgc gcagctttac ttacgatgat aaataccggg
      121 gccggatggt ggcgcaccgt cacacgggcc ggccggaaat ccgttaccgt tacgacagcg
      181 acgggcgggt gacagaacag cttaacccgg caggcttaag ctacacgtat cagtatgaga
      241 aggaccgcat caccatcacc gacagcctga accgccgtga agtgctgcac acgtagggtg
      301 aagccgggct gaagcgggtg gtgaaaaagg aacacgcgga cggcagcgtc acgcagagtc
      361 agtttgacgc cgtgggcagg ctcaggacac agacggatgc cgcaggccgg acaacagaat
      421 acagcccgga tgtggtgacg ggcctcatca cgcgcatcac cacgccggat ggcagggcat
      481 cggcgtttta ctataaccac cacagccagt taacgtcagc caccgggcct gacgggctgg
      541 aaatgcgccg caaatatgat gaatccggcc gtctgattca ggaaaccgcc cctgacggcg
      601 atatcacccg ctaccgttat gataatccac acagtgactt accctgcgca acggaagatg
      661 ccaccggcag ccggaaaacc atgacatgga gccgttacgg tcagttgctg accgtcactg
      721 actgttccgg ttatgtaacc cgttatgacc atgaccgttt tggtcaggtg acggcggtgc
      781 accgcgagga agggctgagc cagtaccgcg catacgacag ccgtggacag ttaattgcgg
      841 tgaaagacac gcagggccat gaaatgcggt atgaatacaa cgccgccggt gacctgaccg
      901 ccgtcattgc cccggacggc agcagaaacg ggacacagta cgatgcgtgg ggaaaagcca
      961 tctgtaccac gcagggcggg ctgacgcgca gtatggaata cgatgctgcc ggacgggtca
     1021 tccgtctgac cagtgaaaac ggcagccaca ccaccttccg ttacgatgta ctcgaccggc
     1081 tgatacagga aaccggcttt gacggccgca cacagcgtta tcaccacgac ctgaccggca
     1141 aacttatccg cagcgaggat gaggggctgg tcacccactg gcactatgac gaagcagacc
     1201 gcctcacgca ccgcaccgtg aagggtgaaa ccgcagagcg gtggcggta
//
LOCUS       DAZPBM010000363         1233 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPBM010000363 DAZPBM010000000
VERSION     DAZPBM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.366"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            55..1170
                     /locus_tag="ACHUZQ_004464"
                     /pseudo
     CDS             55..1170
                     /locus_tag="ACHUZQ_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          485 a          235 c          180 g          333 t
ORIGIN      
        1 taattatcca atttaaacat cttagcataa aacaaatgat gaataaggaa ttttatgtca
       61 attgacttta ccccaggtat aataaataca tatcacggcg atatttataa ctgcacaaca
      121 aataccgata atgctaaaac gccagacaca ccgaagtggc cttgcgataa ttgggaagaa
      181 caacaaccca ttaattccac attctctgga gaaggatata tctctgatca atatgattta
      241 gtgcaacacc aacttcaaca aattaatgcg tgccacacga acactacata tacaaatgca
      301 gactactcaa aagttgtggc tcaacttgta agtcttatta ctaacattga aacgataagt
      361 tcgacacaac ttacgcaaca gacacaatca atacttaatc agattaataa cattagatat
      421 gaaaaaaaca aaagtgctga atgtcgaatc attgttgtcg ctaatcctaa acccgacaag
      481 gcaatcataa ctaaaattag cgtagaagag ggaataccca taacattcag tgtccaaacc
      541 atgttttcag atactaattt tattgctgaa caacgagctg acttaccaac taatataaaa
      601 gatattcaaa gcctgtatca aaaaatgacg aagttatata ttgagcacag tgaaaataaa
      661 aacaggatga aagtctttgc cggagccaac tttattgatt tcaatatgac aggacagaac
      721 ctgtccgggt tcgttctaac cttgtcaagg ttttatttcg aagatctact taatataaat
      781 ttcactgacg caaatttagg tgacactatt ttttacataa agaacaccct acccccaaat
      841 tatataaaga tggacaatat cttgacaaac aaatcgaagg tttattttca acattattaa
      901 ccattaatga caacttttta cgagcaaaag cggaaattgc ttcgacaata attaaatttt
      961 tagaggccag gataaccaat ctttcataca atgacatatt aaaatatcag caagaattcc
     1021 aaaaacaatg ttataagcaa gttaaagcct ttacgacact ctctcgatac aataaaattc
     1081 aaacatgggc agaaatgtct gaatatcagt tcgaagtatt tcaatacgag acgcttaacc
     1141 ccaaaaaaat gtcacacacg ccttatttaa agaggccact tcctaatgaa aaagatataa
     1201 attatggggt ggaaatagaa ataccatctg gca
//
LOCUS       DAZPBM010000364         1231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPBM010000364 DAZPBM010000000
VERSION     DAZPBM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.367"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..1165)
                     /locus_tag="ACHUZQ_004465"
     CDS             complement(<1..1165)
                     /locus_tag="ACHUZQ_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1027960.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCC"
BASE COUNT          400 a          233 c          182 g          416 t
ORIGIN      
        1 aacaacattt tttcaatatg ctttcattca caacagtgtg tgataagtag agattactta
       61 tgttttgctt tattaatgcc acgtcattaa aatgactata tttaatgaaa aaaccatcat
      121 tgagtagtgt tttaagattg actaaattac ttgcatcttc acgtaccatc gtggcaactg
      181 gaacaggcag agacatgaaa acttcttcat tcaccatcaa gtgaatcatg cattgcgttc
      241 cctttgtcat atccacacat acgctacaaa tatcgtggta atgcgaagag cgcatttcag
      301 gaaacagatt aaacacggta tcttgaaagg cactaaatcg tgttaataaa tcctgataat
      361 gaacaggttt tgtttcaaaa ttatcatcag caaatccaag cactattgta tgctctacat
      421 ctaacgcagt ttcaaatggc tcttcttctg taaaatcatt atttaaaacc tcacctgacg
      481 tattatcact gtcataaaaa gtgttcatca atgcgatatt atttgttaca gcgtcttttt
      541 cgatattctc actaattgca tatcgcctgc tataatcata aaaatgaata aacgcgccct
      601 ttataaagga tatatattgt tctttgttaa agtcaatacg gctgtataat ttttcaaaaa
      661 tcacattcgc aattttacta ccaatggtat gcctgtataa taatgggtgc gattcaatcg
      721 taaaatgctt gtctaattga tcttgtaatt tattctcata gtccagaggg aaaagaaact
      781 gttgtctttc aagtttccca ttcgatacat tacgtattcc aatagagatt ctttcattac
      841 ccgcctttat ttcaacttcg ttatcttctg atttacccag atatttttta acatcagtct
      901 cgaaagcatt actaaaaaaa gtattaaatt ggtaattcgc tgatggcata ttttccgcga
      961 gcaacacatc attttttatg agatcactgt cagcatttat tgctaatatc agatgcaagt
     1021 agatggtatc ccatgtagac aactcaccct tacgactgga atttaatatc gcgaaaaatt
     1081 ccctcagtga atccaggaaa ttatcacttt gatttagttg tgtgcctgga aatgatgatg
     1141 cttgatcaga tatattgttt atcatattaa aaatcaccca agaaaaacga taatccaccg
     1201 aactcctcgt gctttataat tataaaagca c
//
LOCUS       DAZPBM010000365         1200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPBM010000365 DAZPBM010000000
VERSION     DAZPBM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.368"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..900)
                     /gene="tdcA"
                     /locus_tag="ACHUZQ_004466"
     CDS             complement(<1..900)
                     /gene="tdcA"
                     /locus_tag="ACHUZQ_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1027961.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSY"
BASE COUNT          386 a          261 c          222 g          331 t
ORIGIN      
        1 ataagatgaa tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata
       61 atttttcgac cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt
      121 aataaattga ttagaaccaa aaggtgacgt catatcacaa ggaattacag ttaagaaatc
      181 agcattgaga acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat
      241 actgatgcca tttctttgta acgtagtaag cagttcgctg tagtacccca tattagtttg
      301 tggcaacacc cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg
      361 ggacttactg gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg
      421 aagcttcatt tctgcactta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc
      481 cggtaagaac gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc
      541 tttgaacttg ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa
      601 tgagacttcc accaccgcct cagaagacat accgcttatc tcattaacca tatttttcat
      661 ttcacgggta atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac
      721 accggtattt ttccgcacca ctaattccac accaaaataa tcttcaatat cgttaatgat
      781 tttactgacg gccggttgag ttaaccctaa ttcttttgca gccgagccga tagagccact
      841 tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat
      901 aacgacctac gttaattacc tcattgacgg catgaagtgt atcaaaatga aatgaacagg
      961 atatgtgcga ccactcacaa attaactttc aatactttcc agggtatcgt cattacaatt
     1021 aaataccttt aaatatcaac aagttaaagc ataaaaatca gcataaaccc tgatttttgt
     1081 caaaaaaaat gacggggata accatatttt atggcgataa catcattcgt tatgtggtta
     1141 atacaaaaaa ggggctgaga ccatatttca agcaattacc ggataattac caggcaaaat
//
LOCUS       DAZPBM010000366         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPBM010000366 DAZPBM010000000
VERSION     DAZPBM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.369"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHUZQ_004467"
     CDS             complement(<1..755)
                     /locus_tag="ACHUZQ_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR1027962.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHUZQ_004468"
     CDS             1071..>1187
                     /locus_tag="ACHUZQ_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027963.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZPBM010000367         1170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPBM010000367 DAZPBM010000000
VERSION     DAZPBM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.370"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>1170
                     /gene="fimD"
                     /locus_tag="ACHUZQ_004469"
     CDS             <1..>1170
                     /gene="fimD"
                     /locus_tag="ACHUZQ_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1027964.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          331 a          261 c          283 g          295 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg
//
LOCUS       DAZPBM010000368         1161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPBM010000368 DAZPBM010000000
VERSION     DAZPBM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.371"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHUZQ_004470"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHUZQ_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR1027965.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1161)
                     /locus_tag="ACHUZQ_004471"
     CDS             complement(510..>1161)
                     /locus_tag="ACHUZQ_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR1027966.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          351 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa t
//
LOCUS       DAZPBM010000369         1157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPBM010000369 DAZPBM010000000
VERSION     DAZPBM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.372"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUZQ_004472"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUZQ_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="ACHUZQ_004473"
     CDS             572..>1157
                     /locus_tag="ACHUZQ_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1027967.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAZPBM010000370         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPBM010000370 DAZPBM010000000
VERSION     DAZPBM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.373"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUZQ_004474"
     CDS             <1..122
                     /locus_tag="ACHUZQ_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027968.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHUZQ_004475"
     CDS             119..376
                     /locus_tag="ACHUZQ_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027969.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHUZQ_004476"
     CDS             376..750
                     /locus_tag="ACHUZQ_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1027970.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHUZQ_004477"
     CDS             747..>1148
                     /locus_tag="ACHUZQ_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1027971.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZPBM010000371         1145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPBM010000371 DAZPBM010000000
VERSION     DAZPBM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.374"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHUZQ_004478"
     CDS             <1..187
                     /locus_tag="ACHUZQ_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1027972.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHUZQ_004479"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHUZQ_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1027973.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAZPBM010000372         1145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPBM010000372 DAZPBM010000000
VERSION     DAZPBM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.375"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            98..>1145
                     /gene="phoE"
                     /locus_tag="ACHUZQ_004480"
     CDS             98..>1145
                     /gene="phoE"
                     /locus_tag="ACHUZQ_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1027974.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTY"
BASE COUNT          343 a          228 c          280 g          294 t
ORIGIN      
        1 gtaatatatc tttaacaatc tcaggttaaa aactttcctg ttttcaacgg ggctctcccg
       61 ctgaatattc gcgcgttaat taaaatcagg aatgaaaatg aaaaagagca ctctggcatt
      121 agtggtgatg ggcattgtgg catctgcatc tgtacaggct gcagaaatat ataataaaga
      181 cggtaataaa ctggatgtct atggcaaagt taaagccatg cattatatga gtgataacgc
      241 cagtaaagat ggcgaccaga gttatatccg ttttggtttt aaaggtgaaa cacaaattaa
      301 cgatcaactg actggttatg gtcggtggga agcggagttt gccggaaata aagcggagag
      361 tgatactgca cagcagaaaa cgcgtctcgc ttttgccggg ttgaaatata aagatttggg
      421 ttctttcgat tatggtcgta acctgggcgc gttgtatgac gtggaagcct ggaccgatat
      481 gttcccggaa tttggtggcg attcctcggc gcagaccgac aactttatga ccaaacgcgc
      541 cagcggtctg gcgacgtatc ggaacaccga cttcttcggc gttatcgatg gcctgaactt
      601 aaccctgcaa tatcaaggga aaaacgaaaa ccgcgacgtt aaaaagcaaa acggcgatgg
      661 cttcggcacg tcattgacat atgactttgg cggcagcgat ttcgccatta gtggggccta
      721 taccaactca gatcgcacca acgagcagaa cctgcaaagc cgtggcacag gcaagcgtgc
      781 agaagcatgg gcaacaggtc tgaaatacga tgccaataat atttatctgg caactttcta
      841 ttctgaaaca cgcaaaatga cgccaataac tggcggcttt gccaataaga cacagaactt
      901 tgaagcggtc gctcaatacc agtttgactt tggtctgcgt ccatcgctgg gttatgtctt
      961 atcgaaaggg aaagatattg aaggtatcgg tgatgaagat ctggtcaatt atatcgacgt
     1021 cggtgctacg tattatttca acaaaaatat gtcagcgttt gttgattata aaatcaacca
     1081 actggatagc gataacaaat tgaatattaa taatgatgat attgtcgcgg ttggcatgac
     1141 ctatc
//
LOCUS       DAZPBM010000373         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPBM010000373 DAZPBM010000000
VERSION     DAZPBM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.376"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHUZQ_004481"
     CDS             <1..>1140
                     /locus_tag="ACHUZQ_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1027975.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          361 g          206 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZPBM010000374         1116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPBM010000374 DAZPBM010000000
VERSION     DAZPBM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.377"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            83..1114
                     /locus_tag="ACHUZQ_004482"
     CDS             83..1114
                     /locus_tag="ACHUZQ_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1027976.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          227 t
ORIGIN      
        1 agatcacttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
       61 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      121 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      181 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      241 ccgttcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      301 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttacg
      361 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      421 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      481 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      541 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      601 agagcatccg gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc
      661 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      721 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctgtgtgg gcggcaacag
      781 caaaagttcg gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc
      841 gaaaaccatc acgctgatcg tggacaacta cattatccac aaaagccggg aaacacagag
      901 ctggctgaag gagaacccga agttcagggt catttatcag ccggtttact cgccatgggt
      961 gaatcatgtt gaacggctat ggcaggcact tcacgacaca ataacgcgta atcatcagtg
     1021 cagctcaatg tggcaactgt tgaaaaaagt tcgccatttt atggaaaccg tcagcccatt
     1081 ccccggaggc aaacatgggc tggcaaaagt gtagcg
//
LOCUS       DAZPBM010000375         1115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPBM010000375 DAZPBM010000000
VERSION     DAZPBM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.378"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHUZQ_004483"
     CDS             complement(<1..643)
                     /locus_tag="ACHUZQ_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1027977.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKFFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHUZQ_004484"
     CDS             complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHUZQ_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1027978.1"
                     /translation="LSISWSHYLFYLISEDMTEPHEARRTEYHARYLDIQIVLKGQEG
                     MTFSTQPAGTPDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAP
                     AQVRKAVVKMLMA"
BASE COUNT          323 a          252 c          196 g          344 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 agaactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaaacagg taatgactcc aacttattga tagtg
//
LOCUS       DAZPBM010000376         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPBM010000376 DAZPBM010000000
VERSION     DAZPBM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.379"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHUZQ_004485"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHUZQ_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHUZQ_004486"
     CDS             294..>1087
                     /locus_tag="ACHUZQ_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1027979.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZPBM010000377         1074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPBM010000377 DAZPBM010000000
VERSION     DAZPBM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.380"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..94)
                     /gene="yqcG"
                     /locus_tag="ACHUZQ_004487"
     CDS             complement(<1..94)
                     /gene="yqcG"
                     /locus_tag="ACHUZQ_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR1027980.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            233..904
                     /gene="queE"
                     /locus_tag="ACHUZQ_004488"
     CDS             233..904
                     /gene="queE"
                     /locus_tag="ACHUZQ_004488"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1027981.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          227 c          245 g          306 t
ORIGIN      
        1 cgttatcatt aaaaactaat acagaaaaaa taaatataat tttggtgaat gttttgacat
       61 gattaaatcc attttcttta ttttcttctg acataatacg ttactccctt taattattaa
      121 cgatatgttt cgcatttttc tttaaatctg cctgtaacag aatgttaata agacatgatt
      181 gttgtggcag gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta
      241 cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc
      301 catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac
      361 ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga
      421 gagtgataag tggggggcgg cgagcagtga agacttgttg gctgtcatta gtcgccaggg
      481 atacaccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg atttgctacc
      541 actgactgat ctgctcgaaa agaacggttt tagctgccag atcgaaacca gtggtactca
      601 tgaggtacgc tgcacaccga atacctgggt taccgtatcg ccaaagctga acatgcgcgg
      661 cggctatgac gtgttgtcac aggcactgga gcgagccaac gaaatcaagc atccagtggg
      721 gcgcgtacgc gatattgaag cactggatga actattggca acgctgaccg atgataaacc
      781 gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt tgtgcattga
      841 aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc taaatattgc
      901 ctgattaaac atttataagc gttataaatg ggtggaacct gtaaagactt ctactcattt
      961 atattgtttg tcgcctctga aaactcctcc attttaccca tccagggcta atcattagca
     1021 ttctctacaa attctgtggc attaattttt cgctggagtg aaaattattg cggt
//
LOCUS       DAZPBM010000378         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPBM010000378 DAZPBM010000000
VERSION     DAZPBM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.381"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHUZQ_004489"
     CDS             complement(77..865)
                     /locus_tag="ACHUZQ_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1027982.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHUZQ_004490"
     CDS             complement(905..>1023)
                     /locus_tag="ACHUZQ_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1027983.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZPBM010000379         1009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPBM010000379 DAZPBM010000000
VERSION     DAZPBM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.382"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..164
                     /locus_tag="ACHUZQ_004491"
     CDS             <1..164
                     /locus_tag="ACHUZQ_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1027984.1"
                     /translation="SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAH
                     HQVDIDLED"
     gene            170..>1009
                     /locus_tag="ACHUZQ_004492"
     CDS             170..>1009
                     /locus_tag="ACHUZQ_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1027985.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIA"
BASE COUNT          306 a          229 c          246 g          228 t
ORIGIN      
        1 aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca atcagccacg
       61 catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggctg
      121 aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta tggcgctttt
      181 tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt ttgctggcga
      241 cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta tgcggcaacg
      301 gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag gctctgactg
      361 ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa cccgctcgtt
      421 tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa tgatcatcga
      481 ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc gaaaccgtaa
      541 acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac gctttaccga
      601 tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat atgagtatga
      661 ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa aaatgaaagc
      721 agaaacagaa accggttatg aaccctccat cctgatccag atggagaagc aaatgaacat
      781 agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca cccgtattga
      841 tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg agtttcttaa
      901 cctgggaggt gaacacctcg gcatcgatac ctcacgggat aacagtgaat tatttgccaa
      961 cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgca
//
LOCUS       DAZPBM010000380         1001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPBM010000380 DAZPBM010000000
VERSION     DAZPBM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.383"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            102..404
                     /locus_tag="ACHUZQ_004493"
     CDS             102..404
                     /locus_tag="ACHUZQ_004493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027986.1"
                     /translation="MEKLILLLLLPLTTSAAIPDFKCWDIHNDSFSTDMSFYVTRYLG
                     KMPPPYGAFHYETAPDEKFYRELVITNEAFDDGYNYGSRYLNKEGTLYDDIKCKRL"
     gene            complement(550..>1001)
                     /locus_tag="ACHUZQ_004494"
     CDS             complement(550..>1001)
                     /locus_tag="ACHUZQ_004494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027987.1"
                     /translation="WEKWLQIEFAHYLTSKRKYELHREVTIYLDPIDYPDSKLAKVDL
                     ILREKSTPSDDYIFIEFKCTKGVVPLVRGLNADKNKISAIRQCNYKMRNYLGVGFHLF
                     CEPDDIEFMEKYVTEELKGSYAVFKLCECPVRYNCQCEFNEIGVVLF"
BASE COUNT          321 a          180 c          166 g          334 t
ORIGIN      
        1 ttttacgtaa ttgaaacaaa atcagccata tcgcttgtta attggtattg agactttgta
       61 ggtgttatca tgcaattaaa tcatttgcat tggtatctct tatggaaaaa ttgatattgc
      121 tattattatt acctttaaca acaagtgcag ctattccaga ctttaaatgc tgggatattc
      181 ataacgattc atttagtact gacatgagtt tctacgttac aagatatctc ggtaaaatgc
      241 cgccgcctta tggtgcattt cattatgaaa cagcacctga tgagaaattt tacagggaac
      301 tggtaataac caatgaagca tttgatgatg gatataatta cggttcgaga tatctaaaca
      361 aagaaggaac tttgtacgat gatattaaat gtaagcggct ttgatactca tgattcattg
      421 tatatagctt acaaagtata ttaaccattg cattcaatgg gtccctccat actggcggta
      481 ctatcgaggg ttttcggcag cgggtttaat caattgtgtg tgaacaaaat gatcataccc
      541 gcaccgcatt taaaaaagta ccacaccaat ttcattaaat tcacattggc aattatatct
      601 tactggacac tcgcataatt taaacacggc gtaagagcct ttaagttctt cagttacgta
      661 cttttccata aactcgatat catctggttc acaaaaaaga tgaaatccga caccaagata
      721 attacgcatt ttgtagttac actgtcttat agcactaatt ttatttttgt cagcatttaa
      781 gcccctaact aatggtacta cacctttagt acatttaaac tcaataaaaa tataatcatc
      841 agagggtgtg gatttttcac gcagtatcaa atctacttta gcaagtttac tatcaggata
      901 atctattgga tcgaggtata tcgtaacttc acgatgtagc tcgtacttcc tttttgaagt
      961 tagataatga gcaaattcta tttgcagcca cttttcccaa t
//
LOCUS       DAZPBM010000381          985 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPBM010000381 DAZPBM010000000
VERSION     DAZPBM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.384"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            230..>985
                     /gene="gspA"
                     /locus_tag="ACHUZQ_004495"
     CDS             230..>985
                     /gene="gspA"
                     /locus_tag="ACHUZQ_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR1027988.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          280 a          199 c          237 g          269 t
ORIGIN      
        1 gcatctttat tatgattcgc cagatggaag gggaaacgta gtgtgggcac gatgtatgtt
       61 ctaataaaat agattgggtg ggttgtatat tgacgttagt ataaatagta ttaatgccgc
      121 ttgcatgaat caagcaaaat ttatttatat tagtaatatg tatttaatta ataaatacat
      181 acattatttt tatgattaag gataatcaat aattaaaaca ggaagttcta tgtctacgag
      241 aagagaagtt attctttcct ggttgcgtga gaaacgtcag acctggcgtc tgtgctattt
      301 gttgggtgag gctggaagtg gaaaaacctg gctggcgcag caactgcaaa aagataaaca
      361 tcgccgtgtg attactttaa gcctcgttgt ttcctggcaa ggtaaggccg catggatcgt
      421 taccgacgat aacgcggctg aacagggctg ccgtgacagc gcctggacgc gagatgagat
      481 ggcggggcaa ttactgcatg cgcttcatcg aactgatagt cgctgccctc ttattattat
      541 tgaaaatgct cacctgaatc accgccgcat acttgatgat ctccaacgcg cgatatctct
      601 tattcctgac ggtcaatttt tgctgatcgg cagacccgat cgaaaagtcg aacgtgattt
      661 taaaaaacag ggcattgaac ttgtctcgac aggacgtctg acggagcacg aacttaaggc
      721 aagcatcctt gaaggtcaga atattgacca acccgatctc cttttaacct ccagagttct
      781 gaaacggata gctttattat gtcggggcga tcgcagaaag ctggcgcttg ccggcgaaac
      841 aattcgctta ttacagcaag ccgagcagac cagcgttttc actgcaaaac aatggcgaat
      901 gatttaccgc atattgggtg ataaccgccc ccggaaaatg cagcttgccg tggtaatgag
      961 tggaaccatt atcgctatta cctgt
//
LOCUS       DAZPBM010000382          983 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPBM010000382 DAZPBM010000000
VERSION     DAZPBM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.385"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            84..>983
                     /locus_tag="ACHUZQ_004496"
     CDS             84..>983
                     /locus_tag="ACHUZQ_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR1027989.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIR"
BASE COUNT          324 a          174 c          213 g          272 t
ORIGIN      
        1 attgagatat tccttattac ctaaaactat ttttcatagc ttatatatga tcaaatactc
       61 cttgcataaa taaggagaac aaaatggaac ttaaaaaact gatggaacat atttctatta
      121 tccccgatta cagacaagcc tggaaagtag aacataaatt gtcagatatt ctactgttga
      181 ctatttgtgc cgtcatttct ggtgcagagg gttgggaaga tatagaggat tttggtgaaa
      241 cacatctcga ttttttgaag caatatggtg attttgaaaa tggtattcct gttcacgata
      301 ctattgccag agttgtatcc tgtatcagtc ctgcaaaatt tcacgagtgc tttattaact
      361 ggatgcgtga ctgccattct tcagatgata aagacgtcat tgcaattgat ggaaaaacgc
      421 tccggcactc ttatgacaag agtcgccgca agggagcgat tcatgtcatt agtgcgttct
      481 caacaatgca cagtctggtt ctcgggcaga ttaagacgga tgagaaatcc aatgagatta
      541 cagccattcc tgaacttctt aacatgctgg atattaaagg aaaaatcatc acaactgatg
      601 cgatgggatg ccagaaagat attgcagaga agatacaaaa acagggcggt gattatttat
      661 tcgcggtaaa aggaaaccag gggcggctta ataaagcctt tgaggaaaaa tttccgctga
      721 aagaattaaa taatccagag catgacagtt acgcaatcag tgaaaagagt cacggcagag
      781 aagaaatccg tcttcatatt gtttgcgatg tccctgatga acttattgat ttcacgtttg
      841 aatggaaagg gctgaagaaa ttatgcgtgg cagtctcctt tcggtccata atagcagaac
      901 aaaagaaaga gccagaaatg acggtcagat attatatcag ttctgctgat ttaaccgcag
      961 agaagttcgc cacagcaatc cga
//
LOCUS       DAZPBM010000383          975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPBM010000383 DAZPBM010000000
VERSION     DAZPBM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.386"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHUZQ_004497"
     CDS             complement(<1..906)
                     /locus_tag="ACHUZQ_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR1027990.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          275 a          228 c          197 g          275 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttct
//
LOCUS       DAZPBM010000384          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPBM010000384 DAZPBM010000000
VERSION     DAZPBM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.387"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>974
                     /gene="tssI"
                     /locus_tag="ACHUZQ_004498"
     CDS             <1..>974
                     /gene="tssI"
                     /locus_tag="ACHUZQ_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1027991.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          250 a          261 c          298 g          165 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZPBM010000385          967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPBM010000385 DAZPBM010000000
VERSION     DAZPBM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.388"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            46..967
                     /gene="yfdF"
                     /locus_tag="ACHUZQ_004499"
                     /pseudo
     CDS             46..967
                     /gene="yfdF"
                     /locus_tag="ACHUZQ_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          375 a          167 c          169 g          256 t
ORIGIN      
        1 aggtaatata tatttgtaac aaatcaatca aaatggaata aaatcatgct accatctatt
       61 tcaatcaaca ataccagcgc aacttaccca gaatccatta atgaaaataa caatgatgaa
      121 gttaatggat tagtacaaga gctcaaaaac ctttttaatg gtaaggaagg aataagcacc
      181 tgtattaaac atctacttga gcttataaaa aacgccatac gagtaaacga cgatccttaa
      241 gatttaatat taataattcc tcagttactt atattgatat tggctccaat gatacagacc
      301 atattactat tggtatcgac aaccaagaac caatagaatt acctgcgaac tataaagaca
      361 aagaactcgt ccgtactatc attaatgaca acatagttga gaagactcat gatatcaata
      421 acaaggaaat gatcttcagc gcattaaaag aaatatatga tggagatcct ggttttatct
      481 tcgataagat atcacacaaa ctcagacata cggtaacgga atttgatgag agcgggaata
      541 gcgaaccaac ggacttattt acctggtacg gtaaagataa aaaaggcaac tctctcgcta
      601 ttgtaattaa aaataaaaac ggaaatgatt acttatctct cggttactac gatcaggacg
      661 actaccacat tcaaagagga attcgtatta atggtgatag tctcacccaa tattgtagtg
      721 aaaaagccag gaatgcttca gcaatgcttc agcgtggttt gaaagcagta aagctatcat
      781 ggcagaatca tttgcaacag gttccgatca tcaggttgta aacgagctca acggggaaag
      841 actgagagaa cctaacgaag tttttaaacg tttaggtcga gcaataagat ataattttca
      901 agtggacgat gcaaaattta gacgcgacaa tgtaaaagaa ataatttcta atttattcgc
      961 taacaaa
//
LOCUS       DAZPBM010000386          963 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPBM010000386 DAZPBM010000000
VERSION     DAZPBM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.389"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..227)
                     /locus_tag="ACHUZQ_004500"
     CDS             complement(<1..227)
                     /locus_tag="ACHUZQ_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1027992.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPCPLPFILFR"
     gene            complement(485..>963)
                     /locus_tag="ACHUZQ_004501"
     CDS             complement(485..>963)
                     /locus_tag="ACHUZQ_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR1027993.1"
                     /translation="DSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDC
                     KIRRGNAAELFSGIRHITINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          234 a          261 c          177 g          291 t
ORIGIN      
        1 cggaaaagaa taaacggcag cgggcagggc agcgcaaggt ccgtctcgcc cggcagcacc
       61 ttcgcaccca gcaccgggtt caccgggtta gcataggtaa tccctcccgg acacaccgaa
      121 caggccactc ccgtcggcgc acctatcagc acacccgctg aaccctgcac aatatccagt
      181 cccttgcggg tcatgtcgcc ctgccgcgcc gccggttttc cgctcatggt ttattcctct
      241 tcatctgaat tgatttacat taggttggtt attttctcat acccacaact atattttctc
      301 tctgaattta ttacgtgcaa gacaatcgca gcactctaac taacttcgat gcaatttgat
      361 gttcagaaaa atccctcatt agattattat catgaaatac agctaatcat ctttagctta
      421 tatatttctc aatgtattct tttgaaagga tccgtgccct ttcaaactct tcagcagggc
      481 aagattacga aagcccgctc cccgcaagga ctgacgccag atagtttctg tccatggctg
      541 cttttcgcat cttacgtctt aaccctgcct tgaatacctt atcattcgtc aaaatattaa
      601 tagtaatgtg ccgtatccct gaaaataatt ctgctgcatt tcctcttctt attttgcagt
      661 cgtcttcatt cattaccacg tccagacgcc agtgcagctt attctccacg tgccagtggt
      721 ttcggattgc tgtggcgaac ttttctgcag ttaaatcagc agaactgata taatatctga
      781 ccgtcatttc tggctctttc ttttgttctg ctattattga ccgaaaggag actgccacgc
      841 ataatttctt cagtcctttc cattcaaacg tgaaatcaat aagttcatca gggacatcgc
      901 aaacaatatg aagacggatt tcttctctgc cgtgactctt ttcactgatt gcgtaactgt
      961 cat
//
LOCUS       DAZPBM010000387          961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPBM010000387 DAZPBM010000000
VERSION     DAZPBM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.390"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>961
                     /gene="htrE"
                     /locus_tag="ACHUZQ_004502"
     CDS             <1..>961
                     /gene="htrE"
                     /locus_tag="ACHUZQ_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR1027994.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          195 c          238 g          250 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 a
//
LOCUS       DAZPBM010000388          958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPBM010000388 DAZPBM010000000
VERSION     DAZPBM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.391"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..680)
                     /locus_tag="ACHUZQ_004503"
     CDS             complement(<1..680)
                     /locus_tag="ACHUZQ_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1027995.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          291 a          159 c          199 g          309 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccg
//
LOCUS       DAZPBM010000389          954 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPBM010000389 DAZPBM010000000
VERSION     DAZPBM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..954
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.392"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..378)
                     /locus_tag="ACHUZQ_004504"
     CDS             complement(<1..378)
                     /locus_tag="ACHUZQ_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR1027996.1"
                     /translation="MNNCIFFSISASTVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNK"
     gene            complement(395..>954)
                     /locus_tag="ACHUZQ_004505"
     CDS             complement(395..>954)
                     /locus_tag="ACHUZQ_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1027997.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          274 a          195 c          168 g          317 t
ORIGIN      
        1 tttatttact gtttcaatgc aatcagcggc aagttggagt ggatttagat ttttcagata
       61 tggtcttatg atctttccct tacttaataa atagcatgta taggattcgg aaagtccagc
      121 atctattttg tacctcatta tattagataa ggatgtgaga aattccttgt tatcattcgc
      181 agccatatat gccagtccga gttcagcctc tgcggcctta caggcaaaat agcttagtga
      241 acacgctttt cgtccctctt tagtactggc tgatattagt gcccataata ttgctttgtc
      301 tgccaaatct aactctctct gaaattgtct tgcagtaact gtactcgcag aaatagagaa
      361 aaaaatacaa ttattaataa ctttgccata ttttttagtc cgtaactgtt aatgtgctat
      421 ctggctctga aaatattaac tcatttaatt tttgcatgtc attagatgaa ccagttatac
      481 aaccctcgga acagaatcga ggtcctaaag acggaccgaa agcgtttaaa caactttgtg
      541 ttaaaaatcc atcacgatta tatgtttctg tatttgctga cggtactaaa cgtataccat
      601 taggttttct attggttgct ccaggaccat taacattcca gctccatacc ccttgaggta
      661 ttggacctct attttgtaag tgtgtacaac caggattatt tttacattgc atattattac
      721 cattattacc agaggcgaca ggaatagtaa gcacatcata agagttatca tcattttgcg
      781 tatctgaaac gcatgaaagc atacttaaag ttatgctgta taaacaggtc gctaatccca
      841 acgggtcaat atcagttatc ggattcaacg gatactgata aaaattccat ccccccttca
      901 gtccaatctt ttcgcgccgg cgtgtacacc gggtccatct ggttttgcat ctgc
//
LOCUS       DAZPBM010000390          933 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPBM010000390 DAZPBM010000000
VERSION     DAZPBM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.393"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(195..>933)
                     /locus_tag="ACHUZQ_004506"
     CDS             complement(195..>933)
                     /locus_tag="ACHUZQ_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1027998.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga cca
//
LOCUS       DAZPBM010000391          916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPBM010000391 DAZPBM010000000
VERSION     DAZPBM010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.394"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUZQ_004507"
     CDS             complement(<1..228)
                     /locus_tag="ACHUZQ_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR1027999.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHUZQ_004508"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHUZQ_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(586..>916)
                     /locus_tag="ACHUZQ_004509"
     CDS             complement(586..>916)
                     /locus_tag="ACHUZQ_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028000.1"
                     /translation="RFQFIIYTLTSWSILITLNDINLALSFILVINNTYVNINIGAKR
                     IRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFTTIMLSAIPSA
                     KMSLSAH"
BASE COUNT          300 a          159 c          174 g          283 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgt
//
LOCUS       DAZPBM010000392          906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPBM010000392 DAZPBM010000000
VERSION     DAZPBM010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.395"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHUZQ_004510"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHUZQ_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR1028001.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DAZPBM010000393          905 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPBM010000393 DAZPBM010000000
VERSION     DAZPBM010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.396"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..796
                     /locus_tag="ACHUZQ_004511"
     CDS             <1..796
                     /locus_tag="ACHUZQ_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1028002.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITL
                     EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGY
                     ENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(839..>905)
                     /locus_tag="ACHUZQ_004512"
     CDS             complement(839..>905)
                     /locus_tag="ACHUZQ_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028003.1"
                     /translation="GTDEIMIYVAGRQILKDYQNK"
BASE COUNT          259 a          182 c          197 g          267 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga
      421 tgttctgata aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt
      481 agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa
      541 agagtatcgt atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg
      601 gtcactcact aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa
      661 tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg
      721 taggcaattt aaaaattgtt ttgcggaaca agaaatctta tctgaatttc ctcaaccggt
      781 aagtcttgcc ggataaatct ggcggcgcac tttgttaaaa agctgcgccg cctgcagatt
      841 atttgttctg atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg
      901 taccg
//
LOCUS       DAZPBM010000394          890 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPBM010000394 DAZPBM010000000
VERSION     DAZPBM010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.397"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..570)
                     /locus_tag="ACHUZQ_004513"
     CDS             complement(<1..570)
                     /locus_tag="ACHUZQ_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1028004.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLA"
     gene            complement(570..>890)
                     /locus_tag="ACHUZQ_004514"
     CDS             complement(570..>890)
                     /locus_tag="ACHUZQ_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1028005.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          211 c          194 g          188 t
ORIGIN      
        1 cgcaagaccc attaagatcc ctggcaaata tcccgccata aatagcgatg ctacagaaac
       61 gccgccagct gtcagggaaa acacaattaa cacgtttgag ggcggtatca gtaatccagt
      121 aatacaagat gagacgttta cagcggtaga aaatgccgga tcataacctt gtttggtctg
      181 aatgggattc agtgtgccac caacggcagc agctgccgca acggctgaac cagaaatgga
      241 accaaacatc atattcgcca ggacgttcac atgaccaagt gaaccaggaa cccgaccaac
      301 cattacctgg gcgaggttaa tcaaacgtat cgcaataccg ccgctgttca tcagcgttcc
      361 ggcaaagatg aaaaagggga ttgccaatag ggcaaagtta tccagtccat tagccagtcg
      421 ctgcgaaaca gtgattgccg cgatatccca gggaaacatc aaaagcattg aagctacagt
      481 ggcaataccg attgagaacg aaatgggcac accaataaag accagcacaa aaaaacagcc
      541 aaacagtgta atcgcgatat aactatccat taactggcct cctgcgcagg ttgtttcagt
      601 tgttgaatgc tttgattaat aaaacacaag gcataaaaca tcatcaccaa tccacttaat
      661 ggcagaatga tatatacgta tcccatcggt atttgcatcg cggcagatac ctgtgatgtt
      721 gccagcgttt tatcaattaa tttcaggcca ccagtcacaa tgaccgaacc agcaaaacct
      781 aaaataagaa cgttaatgac tatactaaga agtaattgct tgcgcttatt cagggcgaga
      841 gcaaataaat cgatagagag atgtctctgc gcgccgacgg tatatgcagc
//
LOCUS       DAZPBM010000395          888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZPBM010000395 DAZPBM010000000
VERSION     DAZPBM010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.398"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            463..867
                     /gene="stpA"
                     /locus_tag="ACHUZQ_004515"
     CDS             463..867
                     /gene="stpA"
                     /locus_tag="ACHUZQ_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1028006.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          257 a          196 c          208 g          227 t
ORIGIN      
        1 gcatccacgt tacagacggt cccggcaaaa gtgcctgacc tgacccctat tagcggttac
       61 cagcgcccca ccagacccgg tgacatcacc ccccggatca tggacgactg ggggcagtaa
      121 tcatgccggg tctggctggc taacacccca ttataagggg tacgaataaa gtaacggggg
      181 ttcttttatt ctgttatatt gcatttttat tcacttcggt tttgtcaggc ttgcggaatt
      241 agcgagcaga gagcgccgtt ctgttcacca ataatctaaa ttactcatca aaatggctaa
      301 tatataatct tgaaattatt ctgagagttt cagaaaataa cagctgaaat aatctcgcgc
      361 aagactgtaa atagattaaa ttttgtggaa atataataag tgatcgctta cactacgcga
      421 cgaaatactt ttttgttttg gcgttaaaag gttttcttta ttatgtccgt aatgttacaa
      481 agtttaaata acattcgcac cctccgtgcg atggctcgcg aattctccat tgacgttctt
      541 gaagaaatgc tcgaaaaatt cagggttgtc actaaagaaa gacgtgaaga agaagaacag
      601 cagcagcgtg aactggctga gcgtcaggaa aaaattagca cctggctgga gctgatgaag
      661 gctgacggaa ttaacccgga agagttattg ggtaatagct ctgctgctgc accacgcgct
      721 ggtaaaaaac gccagccgcg tccggcgaaa tataaattca ccgatgttaa cggtgaaact
      781 aaaacctgga ccggtcaggg ccgtacaccg aagccaattg ctcaggcgct ggcagaaggt
      841 aaatctctcg acgatttcct gatctaatta ctgaggccgg atgtcgct
//
LOCUS       DAZPBM010000396          876 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPBM010000396 DAZPBM010000000
VERSION     DAZPBM010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.399"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>876
                     /gene="cadA"
                     /locus_tag="ACHUZQ_004516"
     CDS             <1..>876
                     /gene="cadA"
                     /locus_tag="ACHUZQ_004516"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1028007.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          220 c          212 g          206 t
ORIGIN      
        1 aacttctcac cgatttacga aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa
       61 gtgatttacg aaacccagtc cactcacaaa ctgctggcgg cgttctctca ggcttccatg
      121 atccacgtta aaggtgacgt aaacgaagaa acctttaacg aagcctacat gatgcacacc
      181 accacttctc ctcactacgg tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa
      241 ggcaatgcag gtaaacgttt gatcaacggt tccattgaac gtgcgatcaa attccgtaaa
      301 gagatcaaac gtctgagaac ggaatctgat ggctggttct ttgatgtatg gcagccggat
      361 catatcgata cgactgaatg ctggccgctg cgttctgaca gcacctggca cggcttcaaa
      421 aacatcgata acgagcacat gtatcttgac ccgatcaaag tcaccctgct gactccgggg
      481 atggaaaaag acggcaccat gagcaacttt ggtattccgg ccagcatcgt ggcgaaatac
      541 ctcgacgaac atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc
      601 agcatcggta tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa
      661 cgtgcgttcg acctgaacct gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat
      721 cctgaattct atgaaaacat gcgtattcag gaactggctc agaatatcca caaactgatt
      781 gttcaccaca atctgccgga tctgatgtat cgcgcatttg aagtgctgcc gactatggta
      841 atgactccgt atgctgcgtt ccagaaagag ctgcac
//
LOCUS       DAZPBM010000397          862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPBM010000397 DAZPBM010000000
VERSION     DAZPBM010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.400"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHUZQ_004517"
     CDS             <1..343
                     /locus_tag="ACHUZQ_004517"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1028008.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..776)
                     /locus_tag="ACHUZQ_004518"
                     /pseudo
     CDS             complement(405..776)
                     /locus_tag="ACHUZQ_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
     gene            complement(794..>862)
                     /locus_tag="ACHUZQ_004519"
     CDS             complement(794..>862)
                     /locus_tag="ACHUZQ_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1028009.1"
                     /translation="FSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          234 a          207 c          192 g          229 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaaaacgg
      781 taatgactcc aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat
      841 gcagctccac cgattttgag aa
//
LOCUS       DAZPBM010000398          852 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPBM010000398 DAZPBM010000000
VERSION     DAZPBM010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.401"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..538
                     /locus_tag="ACHUZQ_004520"
     CDS             <1..538
                     /locus_tag="ACHUZQ_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR1028010.1"
                     /translation="DISLRSLARQYGYDESTVRTWVEKGMPTDTDSNARSWIVDNVLK
                     PLRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKTAV
                     RAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIGNKQEEINE
                     DTEESTTDDTTAEDTENE"
     gene            582..>852
                     /locus_tag="ACHUZQ_004521"
     CDS             582..>852
                     /locus_tag="ACHUZQ_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1028011.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
BASE COUNT          312 a          137 c          186 g          217 t
ORIGIN      
        1 ggatatctca ttaaggtcat tagcgagaca gtacggatac gatgaatcta cagttcgaac
       61 ttgggtagaa aagggaatgc cgacagacac tgattcaaac gcaagaagtt ggattgtaga
      121 taatgtacta aagccgttgc gtgatactaa tacaaaagaa caaattgaac aagagcgttt
      181 gaagaagctt tcagcagaac ggcagttagc agaattagaa ttagcagaaa agaatgggct
      241 ggtggtcagt actgaatata tagaacaggt actaacggaa tatctattcc aggtcaaaac
      301 agcagtgcgt gcaataccat caaaaactta tcttgaatta tttgcacaat cagatgcaaa
      361 ggatttacgc gatgtattaa gaacgcatat tgataaaaca ttataccaat taggctcaat
      421 ggaatttgag ttacccgatg acatggaagt aataggaaac aaacaagaag aaattaacga
      481 ggatactgaa gaaagtacta ccgacgatac aaccgccgaa gatacagaaa acgagtgagt
      541 ggattagtaa tggtgtagtt aaatttgtgg acgggccgaa tatgggcctt gattgggttc
      601 cgtttagttt ccaaaaagaa ccaatggata tagctcaaga gcgaagtact aaaaagattg
      661 tacttcaatc ttgttcccag cttttgaaaa ctacagtact acaatcaatt gcatttaacc
      721 taatggctaa tgatccctgt aactttgctt ttggtagtag ttctgaatca gaagtgaaga
      781 aattcaagga tggtaaattt cttccagcta ttgaaaccag ttcagtacta aagccgttag
      841 taaccgataa ga
//
LOCUS       DAZPBM010000399          850 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPBM010000399 DAZPBM010000000
VERSION     DAZPBM010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.402"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(270..>850)
                     /locus_tag="ACHUZQ_004522"
     CDS             complement(270..>850)
                     /locus_tag="ACHUZQ_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1028012.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          251 a          171 c          157 g          271 t
ORIGIN      
        1 ctaatgtcat aagcatccct ttaattatcc cctcaacctc ttgtaacttt cccactcttg
       61 aactccacat agtccaaacg ccggatgcaa aaaaatcaca cgtgcatttc tgaaattgtg
      121 atggaggaag atagtatcaa ttatatgtgc ttataaacgt ccccgattag cgcagaagtc
      181 accggattgt tcaggctcca ataggttgag tatgccgtat ttattttcca aaataaaaat
      241 cgatgctgtt gctccttcat aaaatacttt tactctctgg acgcttttct tagctctctt
      301 ggtgttatct taaaacgatt atggaacctt tcagtaaaac gagaaggaca cttgtaacca
      361 ttttccctgg caatctcgct gatgggtttt actgtcgttt gtatagcaga caacgcatta
      421 tttaacctca cgtcgtccaa tatactttga aaacttactc cctcgcttgc cagacggcga
      481 tgcaatgtgg aaacagaaat gtagagatat ctcgctacct tgtttgctgt ccattttgtt
      541 ccaggttcgg atagcattag gttataacaa cgacttatta atgattgttt actatctgat
      601 aaaagtaaac aattaacatg atgtgctcct aatgaaagta gaacgcccat tgctaagtgt
      661 tcctgaattt gcgttgagaa gcctcgggaa atagatgttt ttagttgctc ccaacaatat
      721 attaactcgg aattctgagg taagcagaaa cctgtcgtgt tacgaatttg atcggttatc
      781 gcataaagat tttggaactt ttcaattaga tcaatgggat aataaagcat ttctgcaaga
      841 taaagacggt
//
LOCUS       DAZPBM010000400          834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPBM010000400 DAZPBM010000000
VERSION     DAZPBM010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.403"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(354..461)
                     /locus_tag="ACHUZQ_004523"
     CDS             complement(354..461)
                     /locus_tag="ACHUZQ_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1028013.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          169 a          252 c          195 g          218 t
ORIGIN      
        1 caacgtttcg cggctattct tgagttgttc aggttcaaga atggcccccg tgatgttgtc
       61 aggtgcatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat
      121 aaagcccccg caacattgcg cctcaccgga acccttccgg cttgacgctg attctacgac
      181 tgtttttctg ttccggaatc cgtcacgtct tcactctgtg ctgcgcattc cgcagaatat
      241 tatccgcttg caaccgttgc atttttctta cgaaaaaggc cgcagagttt cacccctgcg
      301 gcctgatccg ggcgcaaatt gccattacgg cagcctgacg cccgcatggc acgttacttc
      361 cgcttgttca gccagttcac gatcatactg gcaagaatac cagcaatgac cggagccgct
      421 aaatcatgcc agaaggccat gcccagttct gcgagcgtca tatagccgcc tgtattgtaa
      481 tgacaacgtt tcgcggctat tcttgtattg tttggatgca agaatggccc ccgtggtgtt
      541 gtcaggtgca tacctgcaac gtgcgggggt tttctctctc cagcaaccaa tgccaccagg
      601 gattaagccc ccgcaacatt gcgcctcacc ggaatccttc cggcttgacg ctgattctac
      661 gactgttttt ctgttccgga atccgtctca tcttcactct gtgctgcgca ttccgcagaa
      721 tattatccgc ttgcaaccgt tgcatttttc ttacgaaaaa ggccgcagag tttcactcct
      781 gcggcctggt ccgggcgcaa attgccatta cggcagcctg acgcccacat gaca
//
LOCUS       DAZPBM010000401          834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPBM010000401 DAZPBM010000000
VERSION     DAZPBM010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.404"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>834)
                     /locus_tag="ACHUZQ_004524"
     CDS             complement(<1..>834)
                     /locus_tag="ACHUZQ_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1028014.1"
                     /translation="PVGVFGPGWKAPFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEI
                     SYSRSESLWLARGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWP
                     ERVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHL
                     VLTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLT
                     HDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRP
                     ES"
BASE COUNT          139 a          308 c          251 g          136 t
ORIGIN      
        1 gctctccggc ctccccgcat aatggtgcgc caccatccgc ccggcgtgct ccgcatcata
       61 agtaaacccg cgcacctgca tcccgctgcg gtcatacacc gcccgcagtt caccaccggc
      121 cgtgtacgtg tagcgcgcca gcggcgcagt cggctgttca tccgggtatg ccgggtcgtg
      181 tgtcagccat accgcctcca gccggatacc gttatcggca ccgtactctg taccggcggg
      241 cagcgtgtcg gggaaggaag gagctgagag agggcaggga ctgtcagggg aagataacga
      301 agcggtgtgc ggtttacggg cctcttccgc ccgctgcgcc tgtgtggtca gcaccaggtg
      361 aaaacggcgg cccgcgccgt ccatcacccc cgtcaccgcg cccgccacgt caccctccgc
      421 tgcccggtga aaggccagcg tccgcccgaa gccgtccacc acgcccgtca gcacccggta
      481 tgcgggcggc tccggcggca gcacctcgtc cgcccccggc acccgctccg gccagctgag
      541 tatccaccac ggcccctgaa ggctgtttgt cgccaggtac acatgcggac tcaggcgaac
      601 atcttccggc agcacctgcc agagcgcgct tagcggctgc gaactgtgct gcgccgctac
      661 tccgccccgc gccagccaga gtgactcgct gcggctgtag cttatctcgc cgggaaacag
      721 cggctcaaag tgaatgctcc tgccgccgct gtcgttgagt atcaggcctt catcgcgtat
      781 ctgtaagcgg atatcgaagg gcgctttcca gccgggacca aacaccccca ccgg
//
LOCUS       DAZPBM010000402          820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPBM010000402 DAZPBM010000000
VERSION     DAZPBM010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.405"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            261..>820
                     /gene="fimB"
                     /locus_tag="ACHUZQ_004525"
     CDS             261..>820
                     /gene="fimB"
                     /locus_tag="ACHUZQ_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR1028015.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          239 a          151 c          178 g          252 t
ORIGIN      
        1 ttaatgatga gggttattat tagattcgta tcaggttgat aaatatataa aggtacatag
       61 catgcaagag catggcgttt gtatggcaaa gttattataa gtaactatcg ctactccatt
      121 taagttcgct cagaagaact ggtccactta cgttagttat taagcaaacg ttcgctttta
      181 taaacataat caggattaaa atgttggatt attgctaacc cagcacagct agtgcgcgtc
      241 tgtaattata agggaaaacg atgaagaata aggctgataa caaaaaaagg aacttcctta
      301 cccatagtga aatcgagtca ctccttaaag cagcaaatac cgggcctcat gcagcacgta
      361 attattgtct gactttgctt tgttttattc atggtttccg ggcgagtgaa atttgtcgat
      421 tgaggatttc ggatattgat cttaaggcaa agtgtatata tatccatcga ttaaaaaaag
      481 gcttttcaac aacgcacccg ctattgaaca aagaagttca ggctttaaaa aactggttga
      541 gtatccgtac ttcttatccg catgctgaga gcgagtgggt atttttatca cgtaagggga
      601 atccgctttc tcggcaacag ttttaccata ttatctcgac ttccggtggt aatgccgggt
      661 tgtcactgga gattcatccg cacatgttac gccattcgtg tggttttgct ttggcgaata
      721 tgggaataga tacgcgactt atccaggatt atcttgggca tcgcaatatt cgtcatactg
      781 tctggtatac caccagcaat gcagggcgtt tttacggcat
//
LOCUS       DAZPBM010000403          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPBM010000403 DAZPBM010000000
VERSION     DAZPBM010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.406"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(246..>815)
                     /locus_tag="ACHUZQ_004526"
     CDS             complement(246..>815)
                     /locus_tag="ACHUZQ_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1028016.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAZPBM010000404          813 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPBM010000404 DAZPBM010000000
VERSION     DAZPBM010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.407"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>813)
                     /locus_tag="ACHUZQ_004527"
     CDS             complement(<1..>813)
                     /locus_tag="ACHUZQ_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1028017.1"
                     /translation="TETLTKLEKQTYPNFEIVIVNDGSKDNTSNVLREYGLTHSRLII
                     INKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNYLLKMYTRQHETGGDAIYCGLY
                     GHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGCLFIRKQLLEENNLFFDEDLRL
                     GEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSCRTITHYRHESFAHERIYSSVM
                     QLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFELLNQLVESNEKELNDC"
BASE COUNT          262 a          154 c          112 g          285 t
ORIGIN      
        1 acaatcattc aattcttttt cattgctttc aactaattga tttaatagtt caaaatcacc
       61 attaagtaga acattccata aataacgagt tttatgataa gctctatttt gactcaataa
      121 tgtatgaatt tcttctttcc tgttaccttt gtataactgc atcacagaag agtagattct
      181 ttcgtgcgca aatgactcat gtcgataatg agtgatggtt ctacatgagt tcattaagga
      241 attttctcta tagttatgct tatacatata atatggaacc gcatacatat cgcatgtaat
      301 tagcagtcga tagataaaat ccagatcttc tcctagtcgt aaatcttcat caaaaaaaag
      361 attattctct tccagaagtt gttttcttat aaacaagcac cctatatgga atctaacctt
      421 tttataaaaa aagtcgaaaa gtaaagatcc ttcattaaac tctgtattta taggtgagta
      481 agtaacacca tcttttatat aatggccata aagcccacaa taaatcgcat cccctcccgt
      541 ctcatgttgt ctggtataca tcttcagcag atagttagga tctatctcat catcatcatc
      601 cataaaacat ataaactgtc cttgcgcctt gcggatacct gtattcctgg cagacgaaac
      661 accgccattt tctttattga taataattaa tcgagagtgg gttaacccat actctctcaa
      721 aacgtttgat gtgttatctt tagaaccatc attaacaata acaatttcaa aatttgggta
      781 agtttgtttt tctaatttcg ttagagtttc agt
//
LOCUS       DAZPBM010000405          812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPBM010000405 DAZPBM010000000
VERSION     DAZPBM010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.408"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHUZQ_004528"
     CDS             <1..81
                     /locus_tag="ACHUZQ_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1028018.1"
                     /translation="GYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            89..>812
                     /locus_tag="ACHUZQ_004529"
     CDS             89..>812
                     /locus_tag="ACHUZQ_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1028019.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEI"
BASE COUNT          185 a          204 c          263 g          160 t
ORIGIN      
        1 ggctatgact accggcgcga cgatgatgcg ggcctgtgga gttcagccga tctgacttat
       61 gtcattatct atgaaatgtg aggacgctat gcctgtacca aatcctacaa tgccggtgaa
      121 aggtgccggg accaccctgt gggtttataa ggggagcggc gacccttatg cgaacccgct
      181 ttcagacgtt gactggtcgc gtctggcaaa agttaaagac ctgacgcccg gcgaactgac
      241 cgctgagtcc tatgacgaca gctatctcga tgatgaagat gcagactgga ctgcgaccgg
      301 gcaggggcag aaatctgccg gagataccag cttcacgctg gcgtggatgc ccggagagca
      361 ggggcagcag gcgctgctgg cgtggtttaa tgaaggtgat acccgtgcct ataaaatccg
      421 cttcccgaac ggcacggtcg atgtgttccg tggctgggtc agcagtatcg gtaaggcggt
      481 gacggcgaag gaggtgatta cccgcacggt gaaggtcacc aatgtgggac gtccgtcgat
      541 ggcagaagat cgcagcacgg tgacggcgac gaccggcatg acggtgacac ccgccagtgc
      601 ttccgtagtg aaagggcaga gcaccacgct gaccgtggca ttccagccgg atggcgcaac
      661 cgacaagagc ttccgtgcgg tgtctgcgga taaaacaaaa gccaccgtgt cggtcagtgg
      721 tatgaccatc accgtgaaag gtgttgctgc aggcaaggtc aacattccgg tcgtatctgg
      781 taatggtgag tttgctgcgg ttgcagaaat ca
//
LOCUS       DAZPBM010000406          811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPBM010000406 DAZPBM010000000
VERSION     DAZPBM010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.409"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..440)
                     /locus_tag="ACHUZQ_004530"
     CDS             complement(<1..440)
                     /locus_tag="ACHUZQ_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1028020.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARA"
     gene            complement(592..>811)
                     /locus_tag="ACHUZQ_004531"
     CDS             complement(592..>811)
                     /locus_tag="ACHUZQ_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1028021.1"
                     /translation="SGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTES
                     VTIDLAYEGSGSGDWRTDAFIVGIGYRI"
BASE COUNT          183 a          235 c          194 g          199 t
ORIGIN      
        1 gcacgtgctg aggctgcagc atcggttgca tgagttgccg cctcgctggc tgattttttc
       61 gcggctgccg tattctgtgc aaccgcggag gcgttacgtg acacctcttc caccatctgc
      121 tcaaagcggc gcagtgcctc cggtcggaca tcatcctccg tcatggcacc gagaaaatca
      181 ttcagcgtac ctggtctgga accttcatag acggtaatgg tcccggcatg tgaaggcgga
      241 aaaccttcaa ccagcagggt gacgctgtac tgaccatgct caacatccat gctgtaacgt
      301 ccggcttcat ccggattttc agaggccacc gtgttcacca ccatcgtgct gctggttcgt
      361 ctggccttca gcacaatggt gcagttctgt actggttttc ctgtgccatc tttaagcacg
      421 ccagaaattt ttactgtcat acttttccac caataaaaaa agcccgcagc agtgacgcca
      481 cgggcttcag gacagtgtaa ctttacgttt cctcaaacgc agttcacccc ataaggtgga
      541 tgaacctgcg tatcataaca atatttacag aagataaatc ggcgtctgtt gtcagatacg
      601 gtatccgata ccaacaataa atgcatccgt tcgccagtca ccactaccgg aaccttcata
      661 agcaaggtca atggtcacgg attcggtcgg gttaaactgc acgccagccc cccacgccag
      721 agacgtgttg ctgtggcgac cgtcatcact tccggtcagc acatcgtgcg ttttcccctt
      781 gttgtcagtt acgcggagat aatccccgga g
//
LOCUS       DAZPBM010000407          811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPBM010000407 DAZPBM010000000
VERSION     DAZPBM010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.410"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..811
                     /locus_tag="ACHUZQ_004532"
                     /pseudo
     CDS             1..811
                     /locus_tag="ACHUZQ_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          224 a          199 c          217 g          171 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat t
//
LOCUS       DAZPBM010000408          799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPBM010000408 DAZPBM010000000
VERSION     DAZPBM010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.411"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHUZQ_004533"
     CDS             complement(<1..741)
                     /locus_tag="ACHUZQ_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR1028022.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZPBM010000409          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPBM010000409 DAZPBM010000000
VERSION     DAZPBM010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.412"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHUZQ_004534"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHUZQ_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZPBM010000410          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPBM010000410 DAZPBM010000000
VERSION     DAZPBM010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.413"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            41..757
                     /locus_tag="ACHUZQ_004535"
     CDS             41..757
                     /locus_tag="ACHUZQ_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1028023.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          259 a          138 c          170 g          222 t
ORIGIN      
        1 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
       61 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
      121 tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt
      181 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
      241 aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggacgtt atgaaggctt
      301 tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat
      361 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
      421 cagctatatg tttgacaatg ctggtgtgat tgtatttaaa ggatcagacc ctgaccatat
      481 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
      541 cattgttatt tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc
      601 tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
      661 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga
      721 tgtacaaaaa gtttatcata acgtcgcaaa tctctaatta tcttttaaag aaatctgtct
      781 ttacggcag
//
LOCUS       DAZPBM010000411          785 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPBM010000411 DAZPBM010000000
VERSION     DAZPBM010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.414"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..625
                     /gene="yahL"
                     /locus_tag="ACHUZQ_004536"
     CDS             <1..625
                     /gene="yahL"
                     /locus_tag="ACHUZQ_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR1028024.1"
                     /translation="DKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPV
                     KFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDCKCILLE
                     HHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESL
                     VMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          228 a          158 c          168 g          231 t
ORIGIN      
        1 cgacaaactc attcatctca tggctgccgt acgagactgt gagtggtcag acgataacgc
       61 actcaccata aatgtgcagt ttaatgattt cccaggattt tatgactgga tggattaccc
      121 tgatcacccg gttaagtttg tttttcgcat acttgaaaat caaaaaggga cagtttgggt
      181 atatgaccag gatgatgcgt ttcttgatat taaagcaaat gtgcaggctg gacgctttac
      241 cgggcttaag aaattagtgc agtttatcaa ttcagtgcga acagattgca aatgtattct
      301 tcttgaacac catatgcctt tgctgagact tttccctaaa gggaaagagt gtatgcatgt
      361 ggaaaaatgg cttcgcgaaa tgtcgtctat tccggagaca gacgcgccca ttaagcaggc
      421 gcttgcgcat ggattattat tacacttaaa aaatatctac cctgtttttc ctgaatcact
      481 ggtgatgtta ttgctctctg ttctggacgt aaaaacatac agagatgatg cacgattaaa
      541 tgaatggata tctaatagag tgcaggaatt aggtgacaga tattatcctg taaataagca
      601 cgttaaaatc cgctacactc tttagttcag aaagggttcc acctgcaagt tgttattcca
      661 ccagagagtg aatcgcaaag caggtaatca tttcatctgt atatccgtat gccaaatggc
      721 attccaccgc cttttcgccg atattttgtt gtgatcggcg acacttcgct taaaaaagca
      781 ccagc
//
LOCUS       DAZPBM010000412          783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPBM010000412 DAZPBM010000000
VERSION     DAZPBM010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.415"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
BASE COUNT          236 a          160 c          115 g          272 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaacatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgcttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgtgtaat gcctggcata gcaagccctt
      601 ttcttgtgtt gtcaagacat ttggcaacgt tcaaacatct gtttagaaat gccaaattgt
      661 tgatctaaca aataaacctg tataaacaga acaccattta atctattccc ctgtgtaacc
      721 caccacatca cacaataaaa acaaaaaact ttaattatat ttcatagcat acaaaagtca
      781 ata
//
LOCUS       DAZPBM010000413          779 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPBM010000413 DAZPBM010000000
VERSION     DAZPBM010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.416"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>779
                     /locus_tag="ACHUZQ_004537"
     CDS             <1..>779
                     /locus_tag="ACHUZQ_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1028025.1"
                     /translation="ECFINWMRDCHSTDDKDVIAIDGKTLRHSYDKSRRRGAIHVISA
                     FSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGG
                     DYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDEL
                     IDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHV
                     ENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTN"
BASE COUNT          260 a          138 c          189 g          192 t
ORIGIN      
        1 gagtgcttta ttaactggat gcgtgactgc cattctacag atgataaaga cgtcattgca
       61 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      121 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      181 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctggatat taaaggaaaa
      241 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      301 ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      361 aaaaaatttc cgctgacaga attaaataat ccagcgcatg acagttccgc aatgagtgaa
      421 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      481 attgatttca cgtttgaatg gaaagggctg aaaaaattat gcgtggcagt ctcctttcgc
      541 tcaataatag cagagcaaaa gaaagagccc gaaatgatgg tcagatatta tatcagttct
      601 gctgatttaa ccgctgagaa gtttgcgaca gcgattcgaa atcactggca cgtggagaat
      661 aatctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      721 aatgcagcag aattattttc agggatacgg cacattgcta ttaatgtttt gacgaatga
//
LOCUS       DAZPBM010000414          751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPBM010000414 DAZPBM010000000
VERSION     DAZPBM010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.417"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            584..>751
                     /locus_tag="ACHUZQ_004538"
     CDS             584..>751
                     /locus_tag="ACHUZQ_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR1028026.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DAZPBM010000415          747 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPBM010000415 DAZPBM010000000
VERSION     DAZPBM010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.418"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..401
                     /locus_tag="ACHUZQ_004539"
     CDS             <1..401
                     /locus_tag="ACHUZQ_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1028027.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          261 a          139 c          141 g          206 t
ORIGIN      
        1 ctgatgtgat taatgccatt gtcaaaccgt tctatgaacg tcagatgagt aagtcagata
       61 atccaattga taaagttgta aggtcaaagg tggaacccac agaccaagca ttgactggtg
      121 acatgaactc tttagccgct gttttatggg ctatggttca gggcaaatca aaagatgttc
      181 cacagttttc acctgaagca ttaaataatc tgaaaggtcg agtggaaaca ttcacacaag
      241 atttaaaggt tatcaaaccg tcagtaaata ttcagcctca gacaatcaat atcacaactc
      301 agacggtact tgatggaaag gttattgatg aaagaaccac atcgcatatt aatcgtatgc
      361 aggaagatac cttgattagc tcagcttatc cagaggaata aacaatgtta gagctactaa
      421 tatttattgc cacacatggc aatctattaa tctttatcta gtatgacgat tcaactatga
      481 atagtcgaat gctacgtgaa atcttctgaa cagataccaa catcaaaaat tagatttcac
      541 gtagctcacg cttaacagaa taggaataac aacaatgttt gacacaaagc aaattagata
      601 ctcaggtaaa gatggtatct attttcacct tagagataat gtggaatctt ttcttgcctt
      661 aacagctact gaacagatgg actatgactc accgatgatg gtgacaactc aggctatgca
      721 atcggggcaa actatcactg ataatgt
//
LOCUS       DAZPBM010000416          742 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPBM010000416 DAZPBM010000000
VERSION     DAZPBM010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.419"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..516
                     /locus_tag="ACHUZQ_004540"
     CDS             <1..516
                     /locus_tag="ACHUZQ_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR1028028.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     QKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
BASE COUNT          215 a          171 c          194 g          162 t
ORIGIN      
        1 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
       61 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      121 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      181 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      241 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg catcagtccg
      301 gcaatacaaa aaaggcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
      361 caactcaggc tttgtaagag gtatcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
      421 acaattgttg ctgttgcacg tgagctggcg ggttttatct gggatatggg ccgaatagca
      481 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      541 aaggcgacgt agcccggtac aagagtaacc cacgttagcg cttggcgacg gcggaacaaa
      601 gctgattcgc gacgtaagaa agtggcaggc tcatatgacg gacctctgta atgcggtatc
      661 caacccgcga atatcagcgt gattcaccgt cggatttact gctacgtcgc cctcagggaa
      721 ttaaacctaa aaagaaaatc gt
//
LOCUS       DAZPBM010000417          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPBM010000417 DAZPBM010000000
VERSION     DAZPBM010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.420"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /locus_tag="ACHUZQ_004541"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="ACHUZQ_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAZPBM010000418          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPBM010000418 DAZPBM010000000
VERSION     DAZPBM010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.421"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..416)
                     /locus_tag="ACHUZQ_004542"
     CDS             complement(<1..416)
                     /locus_tag="ACHUZQ_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1028029.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAG"
     gene            complement(397..>736)
                     /locus_tag="ACHUZQ_004543"
     CDS             complement(397..>736)
                     /locus_tag="ACHUZQ_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1028030.1"
                     /translation="RRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAV
                     MLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQST
                     EEEKSDSRAA"
BASE COUNT          139 a          245 c          201 g          151 t
ORIGIN      
        1 cccgccacca ttccgcccgg tgtgtccata tcgagcagaa tgccgtccac catcgggtcg
       61 ctggcagcct gttgcagacg ggcgataatg ccgttgtaac cggtcatccc cgagtacggc
      121 tgcagcgccc gcgtccggct gaccagcgtg ccggacaccg gcagcacggc gatgccgttc
      181 attacctgat aactgcgggc ctgtcgtggt ccgtcatcat caccggataa cgccagcgcc
      241 gcgggtgcct ctccggcagt caggctgtcg ccggatactg catccgtcag gcagctgatc
      301 ccaagctggc ctgcaagcgc acaaaagaaa acccgcgcat aggcgggttc aagcatcagc
      361 ggctcattaa aagccatgct ggcaatatgc gggagattac gcagctctgc tgtcactctt
      421 ctcctcctct gttgattgtc gcagcccgga ttcaaatgcc gcagccgccc aggcgggcgg
      481 tttaagacca gctgcgcggc gctccatcgt ttcacggacc tgctgggcaa aaatttcctg
      541 atagtcgtca ccgcgtttcg cgcactcttt ctcgtaggtg ctcagtccgg cttctatcag
      601 catcaccgct tcctgaactt ctttcagacc atcgatggcc atacgaccgg agcctatcca
      661 gtcgcagttc ccccaggcac tgcgggcttc ctgaaagctg aagcgcgctt ttgaaggtaa
      721 cgtcaccacg cggcga
//
LOCUS       DAZPBM010000419          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPBM010000419 DAZPBM010000000
VERSION     DAZPBM010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.422"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHUZQ_004544"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHUZQ_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR1028031.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZPBM010000420          725 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPBM010000420 DAZPBM010000000
VERSION     DAZPBM010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.423"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            9..725
                     /locus_tag="ACHUZQ_004545"
                     /pseudo
     CDS             9..725
                     /locus_tag="ACHUZQ_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          214 a          153 c          207 g          151 t
ORIGIN      
        1 acacgtaggg tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg
       61 tcacgcagag tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc
      121 ggacaacaga atacagcccg gatgtggtga cgggtctcat cacgcgcatc accacgccgg
      181 atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc
      241 ctgacgggct ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga
      301 acccgcatca gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg
      361 gcctgtatta taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg
      421 atccgattgg gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata
      481 gcatggatcc attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat
      541 ttgcattggc aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa
      601 tatgtaagaa gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata
      661 gtgtagactt gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca
      721 ctcac
//
LOCUS       DAZPBM010000421          723 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPBM010000421 DAZPBM010000000
VERSION     DAZPBM010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.424"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>723
                     /locus_tag="ACHUZQ_004546"
     rRNA            <1..>723
                     /locus_tag="ACHUZQ_004546"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          197 a          160 c          221 g          145 t
ORIGIN      
        1 gcgaaggggg gacggagaag gctatgttgg ccgggcgacg gttgtcccgg tttaagcgtg
       61 taggctggtt ttccaggcaa atccggaaaa tcaaggctga ggcgtgatga cgaggcacta
      121 cggtgctgaa gcaacaaatg ccctgcttcc aggaaaagcc tctaagcatc aggtaacatc
      181 aaatcgtacc ccaaaccgac acaggtggtc aggtagagaa taccaaggcg cttgagagaa
      241 ctcgggtgaa ggaactaggc aaaatggtgc cgtaacttcg ggagaaggca cgctgatatg
      301 taggtgaagt cccttgcgga tggagctgaa atcagtcgaa gataccagct ggctgcaact
      361 gtttattaaa aacacagcac tgtgcaaaca cgaaagtgga cgtatacggt gtgacgcctg
      421 cccggtgccg gaaggttaat tgatggggtc agcgcaagcg aagctcttga tcgaagcccc
      481 ggtaaacggc ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt
      541 tccgacctgc acgaatggcg taatgatggc caggctgtct ccacccgaga ctcagtgaaa
      601 ttgaactcgc tgtgaagatg cagtgtaccc gcggcaagac ggaaagaccc cgtgaacctt
      661 tactatagct tgacactgaa cattgagcct tgatgtgtag gataggtggg aggctttgaa
      721 gtg
//
LOCUS       DAZPBM010000422          715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPBM010000422 DAZPBM010000000
VERSION     DAZPBM010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.425"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            169..>715
                     /locus_tag="ACHUZQ_004547"
     CDS             169..>715
                     /locus_tag="ACHUZQ_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1028032.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          272 a          142 c           97 g          204 t
ORIGIN      
        1 aaaaatcagc attcattggt taatctggaa gcgcaaaata acaaactgac aaaaattaat
       61 atttctgata gttataaact gaaatttctt aatcttgatt ataacaagct agcatcgctg
      121 gatgtctccc ggcaagaatc tctgattgag ttaagtgccc atcacaatat gatcactgac
      181 cttatattac acaatcatcc caggatgaaa acaatcactt taaacgacaa ccatattgca
      241 catttaaacg ccaaaaacac tacaaaactg gaatatttaa acttaagcaa taacaattta
      301 ctgccaacca atgacattga tcaactaata tcatcaaaac atctttggca tgtattagtt
      361 aacggcatca acaatgatcc acttgcacaa atgcagtact ggactgcagt aagaaatata
      421 attgatgaca ctaatgaagt gaccattgat ttatcaggac ttaatttaac cactcaacca
      481 ccagggctgc aaaacttcac atctatcaat cttgataata accaactcac acattttgat
      541 gcaaccaact acgatagact cgtaaagcta agtctgaata gtaatgctct tgagtcaata
      601 aattttcctc aaggcagaaa tgtaagtatt acacatatat ctatgaataa taatgctctc
      661 agaaatattg atatagatag gctttcatca gttacttatt ttagtgcggc acata
//
LOCUS       DAZPBM010000423          702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPBM010000423 DAZPBM010000000
VERSION     DAZPBM010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.426"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>702
                     /locus_tag="ACHUZQ_004548"
     CDS             <1..>702
                     /locus_tag="ACHUZQ_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFR1028033.1"
                     /translation="SRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAI
                     NAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLR
                     VTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCY
                     RANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLA
                     EAFEHYNEWHPHSALG"
BASE COUNT          172 a          169 c          216 g          145 t
ORIGIN      
        1 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
       61 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      121 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      181 tgagcgaaaa cctgctgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      241 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      301 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      361 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      421 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
      481 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
      541 ggtgcggagt ccggagagta acggaatagc agagagcttc gtgaaaacga taaagcgtga
      601 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
      661 gttcgagcat tataacgaat ggcatccgca tagtgcgctg gg
//
LOCUS       DAZPBM010000424          701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPBM010000424 DAZPBM010000000
VERSION     DAZPBM010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.427"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            132..>701
                     /locus_tag="ACHUZQ_004549"
     CDS             132..>701
                     /locus_tag="ACHUZQ_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1028034.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELD"
BASE COUNT          196 a          159 c          196 g          150 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga c
//
LOCUS       DAZPBM010000425          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPBM010000425 DAZPBM010000000
VERSION     DAZPBM010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.428"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHUZQ_004550"
     CDS             <1..>698
                     /locus_tag="ACHUZQ_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028035.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAZPBM010000426          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPBM010000426 DAZPBM010000000
VERSION     DAZPBM010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.429"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHUZQ_004551"
     CDS             <1..>695
                     /locus_tag="ACHUZQ_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1028036.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZPBM010000427          694 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPBM010000427 DAZPBM010000000
VERSION     DAZPBM010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.430"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            114..694
                     /locus_tag="ACHUZQ_004552"
                     /pseudo
     CDS             114..694
                     /locus_tag="ACHUZQ_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          169 a          185 c          183 g          157 t
ORIGIN      
        1 tgatgccgcc cgtctgtttc gggttcaccc ttccaccgtg gtgcgtcttc tcgccagaca
       61 ccggatgact gagatcaaac agacataatc tatgcggaga tttacagcag ggcatggttc
      121 acaaatctga cagcgatgaa ttatcggcac tgcgggcaga aaatgctcga atcatcaagc
      181 ccctgttacc tcctgaaccc gccacaccac gggccggacg cccatgggct gagcaccgta
      241 aaatcatcaa tggcatgttc tgggtgttat gttccggtgc accatggcgc gatttacccg
      301 aacgatatgg gtcatggaaa actgtttata accgctttaa ccgatggtca aagtcaggcg
      361 tgattaatat tattttcaac aggttgcttt cgctacttga tgcaaacggc tttattgact
      421 ggtctgccac cgcgctggat ggcagtaata tccgggcgct gaaatgtgcc gccggtgctc
      481 aaaaaaacat cccgatatcg acggagataa tgggccgggt cgctctcgcg gcggttttgg
      541 caccaaaatc catctggcaa cagacggaag tggcctcccg ttaaatatcg tgctaagccc
      601 cggacaagct catgaaagcc agttcgcgca acgacttctg gacgggattg gcgttcagcg
      661 tcagaacggc agcatgaaac gccgtggaca tgcg
//
LOCUS       DAZPBM010000428          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPBM010000428 DAZPBM010000000
VERSION     DAZPBM010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.431"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
BASE COUNT          241 a          105 c          143 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZPBM010000429          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPBM010000429 DAZPBM010000000
VERSION     DAZPBM010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.432"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..683
                     /locus_tag="ACHUZQ_004553"
     rRNA            <1..683
                     /locus_tag="ACHUZQ_004553"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          157 a          151 c          230 g          146 t
ORIGIN      
        1 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
       61 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
      121 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
      181 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
      241 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
      301 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
      361 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
      421 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
      481 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
      541 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
      601 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
      661 aaccggtact aatgaaccgt gagg
//
LOCUS       DAZPBM010000430          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPBM010000430 DAZPBM010000000
VERSION     DAZPBM010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.433"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHUZQ_004554"
     CDS             <1..560
                     /locus_tag="ACHUZQ_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1028037.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHUZQ_004555"
     CDS             complement(616..>684)
                     /locus_tag="ACHUZQ_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028038.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZPBM010000431          681 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPBM010000431 DAZPBM010000000
VERSION     DAZPBM010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.434"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(95..646)
                     /locus_tag="ACHUZQ_004556"
                     /pseudo
     CDS             complement(95..646)
                     /locus_tag="ACHUZQ_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          185 a          179 c          112 g          205 t
ORIGIN      
        1 gatgtatacg tgtttagtac ccgtcacacc agctatgtga cccccttggc aaaaaagcca
       61 aatatcagct acatccatgt aactattcag taacttattt agcaagtctg taataagttg
      121 ctctagagat tcctagctct tcctgaatct gcaacctcgt tttaccttgt gaaataagca
      181 tctgtacagc ctctgaagaa gcttttacag gtcttcccat atgtacccct taagctttag
      241 cacgttcaat accagccttt ctacgctctg ctatcaagct tctttcaagc tgtgccaacg
      301 ttgacataat gcctaacatt gcttcaccca tagcgcttga taagtcaaat ccttctctaa
      361 ggctgataac tgtcacacct ttagcctgta gcgctttaac cgttgataac acatcaattg
      421 tgtcacgtcc aagacggtct acagctacca caactacggt gtcaccttct cgtgcataag
      481 ctaacaaact gctgaaaccg tttcttaaag aagctttgac agaaccactg acagcttcat
      541 cagcgaacca tttagcaatg ttgtaccctg cctcttcaat ttgcatttgc tggttagtga
      601 tggattggtc atctgtactc accctgcaat atccaagtat cgccataatg ttccccttag
      661 aagtctcaaa agttagtctc a
//
LOCUS       DAZPBM010000432          679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPBM010000432 DAZPBM010000000
VERSION     DAZPBM010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.435"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>679
                     /locus_tag="ACHUZQ_004557"
     CDS             <1..>679
                     /locus_tag="ACHUZQ_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1028039.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQL"
BASE COUNT          195 a          170 c          171 g          143 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaatta
//
LOCUS       DAZPBM010000433          671 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPBM010000433 DAZPBM010000000
VERSION     DAZPBM010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.436"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>671
                     /locus_tag="ACHUZQ_004558"
     CDS             <1..>671
                     /locus_tag="ACHUZQ_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1028040.1"
                     /translation="DLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKP
                     LTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPY
                     LLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQT
                     ITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKH
                     LFAHG"
BASE COUNT          204 a          134 c          136 g          197 t
ORIGIN      
        1 ctgatctgcc gacgtcacag ttttggtcgt acgcgacata cttcagggta ttgtcctttg
       61 aatatctgag tgaaagtatt tccacactgc tgtatctgga tgccgatgtc gtttgtaaag
      121 gaagtctgaa gcctctcaca gaaattatat ttaaagatga gtttgctgcg gtcattcctg
      181 acaatgttag tactcaggcg gcatgtgcaa aacgcctcaa cattcccgag atgaatggac
      241 gttatttcaa tgcaggcgtt atctatgtca atcttaaaaa atggcatgaa gcaaatttga
      301 caccgtattt actcacgctt ttacgagggg aaactaaata tggctctctt aaatatttag
      361 atcaggatgc gttgaatatc gcatttaata tgaataatat ctacctcgcg aaggattttg
      421 atactattta taccctgaaa aacgaacttc atgatcgtag tcatcgaaag tatcagcaaa
      481 ccattaccga taaaacagta ttgattcact atacagggat aactaaacca tggcatagct
      541 gggctggtta tccgtctgca tcatacttta atatcgcgcg tgaacaatct ccctggaaga
      601 aatatcctct taaagaggcg cggactgttg cagaaatgca gaaacaatat aagcatctgt
      661 ttgcccatgg t
//
LOCUS       DAZPBM010000434          663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPBM010000434 DAZPBM010000000
VERSION     DAZPBM010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.437"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>663
                     /locus_tag="ACHUZQ_004559"
     CDS             <1..>663
                     /locus_tag="ACHUZQ_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1028041.1"
                     /translation="TFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVPD
                     NSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLNY
                     SFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIH
                     GI"
BASE COUNT          201 a          128 c          145 g          189 t
ORIGIN      
        1 ttacttttat tgcagatgat aataacgcag aactcatccc atgcctgagt acagatcttc
       61 ttgtcagtct tggtataaaa aaaatagcgt tattagacaa taaagaacat tcggcagaaa
      121 aacatgtgcc ggacaacagc gcatgcacac cacttcagga tcgtttggcc gacgcatcaa
      181 ctgagtttga tgttggtcag caacatctgt cactctccgt tccgcaaatt tatgttggca
      241 gaatggctcg cggctatgtt tctcctgatc tgtgggaaga aggaataaat gcagggcttc
      301 taaactatag ttttaatggt aattctatta ataatcgtag taaccataat gcaggaaaat
      361 caaactatgc atatttgaat ttacagagtg gcatcaacat tggtagttgg cgactacgcg
      421 ataactcaac gtggagttat aacagtggga gtagcaattc atctgacagc aataaatggc
      481 agcatatcaa tacgtcggct gaacgtgaca ttattccctt acgctcacgg ttaacggtag
      541 gtgatagtta taccgatggc gatatttttg atagtgtgaa ctttcgtggc ctcaaaataa
      601 attcaacaga agcgatgttg cccgatagac aacatggttt cgctccggtg attcatggta
      661 ttg
//
LOCUS       DAZPBM010000435          651 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPBM010000435 DAZPBM010000000
VERSION     DAZPBM010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.438"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..145)
                     /gene="ykfM"
                     /locus_tag="ACHUZQ_004560"
     CDS             complement(<1..145)
                     /gene="ykfM"
                     /locus_tag="ACHUZQ_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR1028042.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFY"
     gene            complement(155..>651)
                     /locus_tag="ACHUZQ_004561"
     CDS             complement(155..>651)
                     /locus_tag="ACHUZQ_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028043.1"
                     /translation="WLLEKLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIWFSV
                     AWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVILVG
                     GAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMTPFMYVLNT
                     PPVI"
BASE COUNT          223 a          135 c          109 g          184 t
ORIGIN      
        1 cataaaatat ttccgtggag cattttatta ttgaatatag aggtttaact ccggtaaaaa
       61 acaaagaagc attgaatgca gggaaaaata atatggccat aaaaaacatc gaaagaaact
      121 cttttaattt aacatgtaaa cgcatggtta atcctcatat cacgggtgga gtgttaagaa
      181 catacataaa tggagtcatg ttttcccttt tccatttatc aagttcctgt tgccgtttta
      241 gtccatctct aattgcatat tttaattttt ctgataaatg gcattgagca tcgatttcat
      301 ttaaaacaac tgtacacact atccctgtca gtaaaattgc tcctccgaca agaattactg
      361 gaaagccaaa gctaacacag gcaaaagaag ccaggcttcc aatagcggtt gcaacaccac
      421 cggctataac tgctttggcc acatccattg aaaaatcgcc aagaaaatta accagatctc
      481 gctctgaact cataataaat tctatggttc tccaggctac tgaaaaccag atacagaact
      541 taactccttt tattgattca gataaagctc ctcgccagcc aatagcgagt tccagaactt
      601 gaggcgctcg tgaagagaaa atatatttac cgagcttttc taaaagccag g
//
LOCUS       DAZPBM010000436          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPBM010000436 DAZPBM010000000
VERSION     DAZPBM010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.439"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUZQ_004562"
     CDS             <1..196
                     /locus_tag="ACHUZQ_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1028044.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
     gene            complement(197..388)
                     /locus_tag="ACHUZQ_004563"
     CDS             complement(197..388)
                     /locus_tag="ACHUZQ_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1028045.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(493..>650)
                     /locus_tag="ACHUZQ_004564"
     CDS             complement(493..>650)
                     /locus_tag="ACHUZQ_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1028046.1"
                     /translation="TIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSD
                     AWIMRLA"
BASE COUNT          183 a          147 c          162 g          158 t
ORIGIN      
        1 ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg
       61 gcagggaatt acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt
      121 ggatattggc aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct
      181 tgtgcaattg caataattaa aaatcaccgt actcctgtgc aggtcgccag aaaccatcta
      241 taaaatcctc aatcggaaaa caaccgccat ggcggatccg ttgatcgctc atagaataaa
      301 gacactgctg ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt
      361 agcaaacaaa aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa
      421 gtttaggaga gattttacaa cgggggaata accaggacaa ataacccgcc atcccgacgg
      481 gttctttttg gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt
      541 acggtaaagg acagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca
      601 ggcatcgagt tgcatacata aatcggggtc agatttacaa ggaatggtta
//
LOCUS       DAZPBM010000437          649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPBM010000437 DAZPBM010000000
VERSION     DAZPBM010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.440"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHUZQ_004565"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHUZQ_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHUZQ_004566"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHUZQ_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          153 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaac
//
LOCUS       DAZPBM010000438          647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPBM010000438 DAZPBM010000000
VERSION     DAZPBM010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.441"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHUZQ_004567"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHUZQ_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          212 a          146 c          113 g          176 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtattaaata aaaccacctc aattacatta ataaatctaa acaaacatca tgcaaataat
      541 ttccatggtt atttctaggc tatttaaatt aaataaataa attatgagtc aatcataaat
      601 aacaaccttt aatataattt aactaccaaa tttatattcg ttcacaa
//
LOCUS       DAZPBM010000439          644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPBM010000439 DAZPBM010000000
VERSION     DAZPBM010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.443"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>644)
                     /locus_tag="ACHUZQ_004568"
     CDS             complement(<1..>644)
                     /locus_tag="ACHUZQ_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028047.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPA"
BASE COUNT          107 a          211 c          179 g          147 t
ORIGIN      
        1 gccggttcac cgttcaccgt gcgccgggtg atacggtccg acgcatcgta atgccagagg
       61 gtgataagcc cctcatcttc actctgcgtg agttttccgg tcaggtcata ctggtaacgt
      121 tgcgtccggc cgtcaaagcc gcgctgctcc gtcagcctgt cgaccgggtc ataccggaac
      181 gtggactggc tgccgttctc gttggtcagc acagtgatgc gcccggcggc gtcatacccc
      241 atgcttcgcg tcagaccgcc ctgcgtggtg ctgacggcct ttccccacgc atcatactgt
      301 atctcactgc ggctgccgtc cggggcaacg atagcggtga ggtctcctgc ggcgctgtac
      361 tcataacggg tttcacgccc ctgcgcatcc ctctgactga ccagttgacc gcgcgggtta
      421 taactgctgt aagtgctgat gccttcttcc cggtgaacgg cgatttgttg accgtaccgg
      481 tcatactcat accgcgtcgt gtaccccgag cagtccgtaa acgcctgaag ctgaccgtac
      541 cggtttcgcg ttattctccg cctcaccccg gttccgtctt ccaccgcaca gggcaggcca
      601 ctgcgggaag aatcataaaa ccagcgcgta atgttaccgt tgcg
//
LOCUS       DAZPBM010000440          642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPBM010000440 DAZPBM010000000
VERSION     DAZPBM010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.444"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>642
                     /gene="fliD"
                     /locus_tag="ACHUZQ_004569"
     CDS             <1..>642
                     /gene="fliD"
                     /locus_tag="ACHUZQ_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1028048.1"
                     /translation="KVSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDE
                     SSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLV
                     LTASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSS
                     NKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          218 a          146 c          139 g          139 t
ORIGIN      
        1 aaagtttcca ccgaagcagg tgctgccccc ggtatctata ccatcagcgt aacccaactg
       61 gcgcaggcgc aatctttacg aactgattcc ccgacaatca ttgcatcaac aaaagatgca
      121 ctgggtgatg agtcatcaga tacacgcaca ataaaaatta cccaagacgg tcgtaaagag
      181 ccgctggaaa tcaagctcaa taaagatcag acgtcattgg atgagatcag caaagccatt
      241 aacgatgccg atagtggaat ttccgctagt atcgttaaag tcaaagatgg taactatcaa
      301 ttggtattga ccgccagtga aggtctggcc aataaaatga ccatatctgt tgagggtgac
      361 agtaaattaa atgatctgct ggcttatgac agcaagacca acaccgggaa tatgaaggaa
      421 ctggttaacg cgcaaaatgc acagctaaac gttaacggta tcgatattga acgttcaagt
      481 aacaaaatta ccgatgctcc tcagggagtg actctcgatt tgaccaaaaa agtcactgac
      541 gtccgggtca cggtgacaaa aagcaacgat aaagcaaccg aagcaattaa aggctgggtg
      601 gattcatata actccctcat tgacaccttt aacaccctga ca
//
LOCUS       DAZPBM010000441          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPBM010000441 DAZPBM010000000
VERSION     DAZPBM010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.445"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..572)
                     /locus_tag="ACHUZQ_004570"
     CDS             complement(<1..572)
                     /locus_tag="ACHUZQ_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1028049.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDP"
BASE COUNT          161 a          156 c          152 g          171 t
ORIGIN      
        1 gggtccataa acaactgacc ggggcgcata gggatctcac gcagctccac atcccagtag
       61 cgggcgaatt tatgccagca gatttgtacc ggaccgcaca ccaggtttgg tttatccgtt
      121 ggtttgcctg cagcttccat acgcttgcgc caacgccatt tcatcgccat cccgccgagc
      181 atacaggcct cggaagaacc aatggtgttg gtgccaacgg cctgaccatt tttcggcgca
      241 ggcgcatgcc acagatcggc aaccatattt acgcaacgca ggtcgatggc tgcggattgc
      301 ggatattctt ctttgtcgat ccagtttttg ttaatggata aatccatcaa tttgtggaca
      361 ttttcgtcgt cccaggtctg gcagaaagtg gccaggttct gacgagcgtt gccatcaaga
      421 tataattcgt cattgataat ctggaatgcg acatcgtcgc gcatttcgtg cagcggaaaa
      481 cgttttgatt ctgcgatagt ggaaatagac ttcgcaccaa aacgtgaatc gagtagttcc
      541 gaccttaaat ccgttacttg cttcttatcc attttaaact ccttaaaatg attggatcgc
      601 attaaaaaag taggatttat cgataaagta agcaagtgca
//
LOCUS       DAZPBM010000442          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPBM010000442 DAZPBM010000000
VERSION     DAZPBM010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.446"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(1..607)
                     /locus_tag="ACHUZQ_004571"
                     /pseudo
     CDS             complement(1..607)
                     /locus_tag="ACHUZQ_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          190 a          160 c          145 g          145 t
ORIGIN      
        1 tactaccaat ccgattctgt acactatttc cgctgaaatt ataattgagc aagccggcat
       61 taataccggg atcccacaac tcaggaggaa tatatccacg cgcgcgatta ctcataaatg
      121 cctgagggat cgtcaggttg agtcgctgct gaccaacatc taaatgcgca gtagcgtcat
      181 ggatcattga ggttaatggc acgcaggcat catccaccag cagattcata ccggagacag
      241 aagccgtatt cagccccata ctggcaagct gcgcgcgtgt caggcaggga acaatccctt
      301 gttcactgtc gcccgtgtta aatgtgacat cacgcgttgc catataacca ttattcaaat
      361 agatatcgac gcgatacgtc cctggcggta attcttgccc attttcaaaa cgcgataaat
      421 cggccacagc ctagggatca tccgctaaaa agcgcggatt aaaatagagt tcggcagatg
      481 acaaaggtgc ctgtgcggca aaagcacagg cgacaaagag ccggacaaaa agaccagcca
      541 aacgatgctt acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat
      601 atgacattat tcctccggtt taaaatcacc cggcagtcaa
//
LOCUS       DAZPBM010000443          625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPBM010000443 DAZPBM010000000
VERSION     DAZPBM010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.447"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            173..>625
                     /gene="ygeH"
                     /locus_tag="ACHUZQ_004572"
     CDS             173..>625
                     /gene="ygeH"
                     /locus_tag="ACHUZQ_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFR1028050.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKID"
BASE COUNT          222 a           98 c          104 g          201 t
ORIGIN      
        1 ataaggctat ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata
       61 gatatgtcga atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt
      121 tctattgctt aagcaggatg caagaaacca attttttcat agaggttaac taatggactt
      181 agaaaataaa ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct
      241 tactcaagga aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt
      301 acttgaatcc gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa
      361 tattattgtt agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga
      421 aaaaattggc tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg
      481 gcaggttttc aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt
      541 ccctttcact acttcattga atacactgga tccattaata cttaatcagg aattagtgca
      601 aatcatttca aataaaaaaa tcgat
//
LOCUS       DAZPBM010000444          614 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPBM010000444 DAZPBM010000000
VERSION     DAZPBM010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.448"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUZQ_004573"
     CDS             complement(76..423)
                     /gene="tnpB"
                     /locus_tag="ACHUZQ_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1028051.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(420..>614)
                     /locus_tag="ACHUZQ_004574"
     CDS             complement(420..>614)
                     /locus_tag="ACHUZQ_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1028052.1"
                     /translation="LPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISSEVTFRHGTL
                     RFNGNVSEKLLTLLIQELKR"
BASE COUNT          135 a          165 c          175 g          139 t
ORIGIN      
        1 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
       61 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttag gctgtcgcca
      121 gtcgatacct tccagcagca tcgccagctg cgcctgtgta aggaacactt tgccatcacg
      181 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
      241 actggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaacatg
      301 gccggacatg ggatcgtctt tcagcgccgt ctgtactttc gcagccaggc cgttgaagcc
      361 atttctcata tcggtgatac cggcaaccag ccaaattttg atcccggaag gtaacgggat
      421 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
      481 cggagcgtcc cgtgccggaa cgttacctca ctgctgatac tgagggtttt cgggtcctct
      541 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc
      601 tctgaagaag gtaa
//
LOCUS       DAZPBM010000445          602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPBM010000445 DAZPBM010000000
VERSION     DAZPBM010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.449"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            10..549
                     /locus_tag="ACHUZQ_004575"
     CDS             10..549
                     /locus_tag="ACHUZQ_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR1028053.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT          152 a          118 c          160 g          172 t
ORIGIN      
        1 gagaatcaga tgaactggca ttatgaaaaa aatggtgtac gttatgataa cgtaacagag
       61 gaagatatta tcgaacgtat ccggcgtggt gaactgaatg catcaacact ggtgtggcaa
      121 cagggcatgc tggaatggca gccagtttca gccacatcac tggcggaaat actgaaacaa
      181 agtgaagttc ccccagccct tccgggtcac cgcattcccg gaggcgttgt ctggacgctg
      241 gcatttgccc ctttgattgg gtacatactt gagatgtgga ctgcagggct gaacggtatg
      301 tcatttgaac gggcttatga tgcagtcagc gaaggccagt actggtttat ctctctgatt
      361 ctgaatattg ctctgggata tctggatgaa cgaaaactcc gtaaatctgg tgttgatact
      421 gcaacatttg gctggctggc atggttgatc ccggtttatc tgtggcgtcg ggcaaaaatc
      481 ctcggacaaa aacctgcgta tttctgggtc tggttggtta tgctgatact ggtgttattt
      541 acggtctgat attctttatc gcagagttta ctaaataatc aggttattag tgttcttaac
      601 ag
//
LOCUS       DAZPBM010000446          594 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPBM010000446 DAZPBM010000000
VERSION     DAZPBM010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.450"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHUZQ_004576"
     CDS             <1..91
                     /locus_tag="ACHUZQ_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028054.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>594
                     /gene="mcbR"
                     /locus_tag="ACHUZQ_004577"
     CDS             289..>594
                     /gene="mcbR"
                     /locus_tag="ACHUZQ_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1028055.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     "
BASE COUNT          173 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tgaa
//
LOCUS       DAZPBM010000447          587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPBM010000447 DAZPBM010000000
VERSION     DAZPBM010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.451"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHUZQ_004578"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHUZQ_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHUZQ_004579"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHUZQ_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          194 a          114 c          119 g          160 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgc
//
LOCUS       DAZPBM010000448          581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPBM010000448 DAZPBM010000000
VERSION     DAZPBM010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.453"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..502
                     /locus_tag="ACHUZQ_004580"
     CDS             <1..502
                     /locus_tag="ACHUZQ_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFR1028056.1"
                     /translation="ISDPNEPKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGT
                     PCNQTGEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQH
                     NDSNNETGEDELNWKRQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSD
                     ERLPDF"
     gene            480..>581
                     /locus_tag="ACHUZQ_004581"
     CDS             480..>581
                     /locus_tag="ACHUZQ_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1028057.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADD"
BASE COUNT          196 a          119 c          155 g          111 t
ORIGIN      
        1 gattagcgac ccgaatgaac cgaaatggat tcccggtcat catatctcct caaatgaagt
       61 caaaaaaacg gaaaatgaag tgcgacagac tgaagataaa cagcaccaga acagcgaacc
      121 ggaggaggga acaccctgca atcagactgg cgaggataac tgccctgact gtggcgcggt
      181 gatgggagac gcaacttatc aggaaacttt tgacgaaaaa aaaccagatg aagcacagga
      241 agaagagccg aagaaaacgg aaaaagctga tgatttgtta ccggagaacg ctggcagcga
      301 tcagcataat gatagcaata atgaaactgg cgaagatgaa ctcaactgga aaagacaggt
      361 gttgattgcg gcggtttacg gtttatgtgc gaatcctgcg tgcatagcct atgcgcctgc
      421 aatccctgac atcgcaatga tgattgccaa caagcttgaa aattttggag tgaccagtga
      481 tgagcgctta cccgattttt gatcgtattg aagaaatggc atgggctcgt cactaccagc
      541 agatcgttcg tgaagaaaaa gaaacggaac tggcggacga c
//
LOCUS       DAZPBM010000449          572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPBM010000449 DAZPBM010000000
VERSION     DAZPBM010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.454"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHUZQ_004582"
     CDS             complement(<1..396)
                     /locus_tag="ACHUZQ_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1028058.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          165 a          146 c           93 g          168 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcatccatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gt
//
LOCUS       DAZPBM010000450          570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPBM010000450 DAZPBM010000000
VERSION     DAZPBM010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.455"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..200)
                     /locus_tag="ACHUZQ_004583"
     CDS             complement(<1..200)
                     /locus_tag="ACHUZQ_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1028059.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKA"
BASE COUNT          160 a          148 c          112 g          150 t
ORIGIN      
        1 gccttacgct tgtcttctct tattttgaaa tacagatttg tcagataagt cagaagcccc
       61 agaagcagac ttcccagcac accaatcgca gcccactgtg atggactgac ctgatccaac
      121 cactgcaaaa accagtagcc ggcactgccg gcggaggtgc cgtaggcaat gcctgttgat
      181 attttttcca tctgatacat atcccgcccc ccgacagacc tgtgctatcg gaaagaaaaa
      241 aggccatcag caaaactctg atggcctgaa tcacctttac caatattgta tgaaaaaaca
      301 cgcacgacta attgacaata attttcattt ccattaaaaa agatcgcgta gcattcttaa
      361 ttcatgagga actttacccg ccagcaatct gagtagcgaa agctgttcat ccccaatgtt
      421 tttgctggcg ggtccttttt tctacggtcc tcctccccgg aggggggcat ttcattattt
      481 ttctaatcgt tactggtgag aaggcggaag aaaaagccag ttcttcgccc ctaaataaca
      541 gcatgattcc agtccatgca aaatctgatt
//
LOCUS       DAZPBM010000451          561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPBM010000451 DAZPBM010000000
VERSION     DAZPBM010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.456"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>561)
                     /locus_tag="ACHUZQ_004584"
     CDS             complement(<1..>561)
                     /locus_tag="ACHUZQ_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR1028060.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLW
                     QDEHLLRLCLRQALQLADVVKLSEEE"
BASE COUNT          146 a          167 c          128 g          120 t
ORIGIN      
        1 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
       61 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag ctgacaaaac
      121 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
      181 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
      241 tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt tccccttgat
      301 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
      361 tatcgactcc ctcagttagc agcgttcttt gcattaacgc cccaaaagga tcatcaccga
      421 cccgacctat aaatccactt attccgccta atctggcgat tcccaccgca acgttagctg
      481 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctacgaccg
      541 catcccctaa aacccatact t
//
LOCUS       DAZPBM010000452          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPBM010000452 DAZPBM010000000
VERSION     DAZPBM010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.457"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..428
                     /locus_tag="ACHUZQ_004585"
     CDS             <1..428
                     /locus_tag="ACHUZQ_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1028061.1"
                     /translation="QLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGRE
                     FAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKK
                     RRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          163 a          124 c          156 g          116 t
ORIGIN      
        1 aacagcttac cagcgtggcg aggcagaatg aaacctgtaa gcggttgctg gatattcctg
       61 gcgttggacc acttattgcg acggcagcgg tggccaccat gggggaagca tcagcgttta
      121 aatcggggcg agagttcgcc gcatatgttg gtctggttcc aaaacaaact ggctccggag
      181 ggaaagtacg tctgctgggg ataagcaaac gtggtgacac ttatctcagg acattattta
      241 tccacggtgc aagagcggtg gcattagtag ctaaagagcc tggcccgtgg ataaccgaac
      301 tgaaaaaacg tcgtccagcc agtgtggcaa tcgtcgccat ggcaaacaag ctggcacgaa
      361 cagtatgggc gataaccgcc catgaccgta agtatgacag gaaccacgtc agtatcagac
      421 catattaatc gctgatacca ttaaacaatg aactcttaac aaaagggtga atgctgaaag
      481 gttgctatgg cggccagagt gatgacaaag acaggtaaga ccgtgactca ctaaacctga
      541 acagtatttt gggcttgaa
//
LOCUS       DAZPBM010000453          555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPBM010000453 DAZPBM010000000
VERSION     DAZPBM010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.458"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            78..555
                     /locus_tag="ACHUZQ_004586"
                     /pseudo
     CDS             78..555
                     /locus_tag="ACHUZQ_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT          136 a          121 c          123 g          175 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagca
//
LOCUS       DAZPBM010000454          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPBM010000454 DAZPBM010000000
VERSION     DAZPBM010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.460"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>553
                     /gene="feaR"
                     /locus_tag="ACHUZQ_004587"
     CDS             <1..>553
                     /gene="feaR"
                     /locus_tag="ACHUZQ_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR1028062.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          142 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aat
//
LOCUS       DAZPBM010000455          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPBM010000455 DAZPBM010000000
VERSION     DAZPBM010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.461"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHUZQ_004588"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHUZQ_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZPBM010000456          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPBM010000456 DAZPBM010000000
VERSION     DAZPBM010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.462"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            38..>546
                     /locus_tag="ACHUZQ_004589"
     CDS             38..>546
                     /locus_tag="ACHUZQ_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1028063.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNV"
BASE COUNT          159 a          111 c          131 g          145 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaa
//
LOCUS       DAZPBM010000457          534 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPBM010000457 DAZPBM010000000
VERSION     DAZPBM010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.463"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHUZQ_004590"
     CDS             <1..275
                     /locus_tag="ACHUZQ_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFR1028064.1"
                     /translation="KMAQVIAKLLPLQLLRGVGLEQRQHVHLARKSLVEAVNADQIPD
                     LYGSRVPCLQATMHGLPVATRPKIKMVQLQFTPFASDDTYHWQTTM"
     gene            complement(254..>534)
                     /locus_tag="ACHUZQ_004591"
     CDS             complement(254..>534)
                     /locus_tag="ACHUZQ_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shufflon protein D'"
                     /protein_id="HFR1028065.1"
                     /translation="WRRASGSTVLTGKIANGQQIPLPSGFSASQCTWSVSNAENPHGW
                     KPNYFAGSVATYDANRIVKCGFYDEYNFYGGTHRTDLSGKCSYIVVCQ"
BASE COUNT          142 a          135 c          122 g          135 t
ORIGIN      
        1 aaaaaatggc gcaggtgata gccaaattgt taccgcttca gctactgcgt ggcgttggcc
       61 tggagcaacg gcaacatgtc catctggcaa gaaagtcatt ggtggaggcg gtcaatgcag
      121 atcaaatacc ggatttatat ggctcacgcg ttccatgcct tcaggcaaca atgcatggac
      181 tgccagttgc gacacgaccg aagatcaaaa tggttcaatt acagtttacg ccatttgcca
      241 gtgacgatac ttatcactgg caaaccacga tgtaactaca tttccccgac aggtcagttc
      301 ggtgagtacc gccataaaag ttgtattcgt catagaagcc gcatttcaca attcgattgg
      361 catcataggt tgcaactgac cccgcaaaat aattcggctt ccacccgtgc ggattttctg
      421 cattactgac actccaggta cactggctgg cagaaaaacc agatggcaat gggatctgtt
      481 gtccatttgc tatttttcct gtcagaacag tgctacctga tgcccttctc cacg
//
LOCUS       DAZPBM010000458          534 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPBM010000458 DAZPBM010000000
VERSION     DAZPBM010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.464"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(114..534)
                     /locus_tag="ACHUZQ_004592"
                     /pseudo
     CDS             complement(114..534)
                     /locus_tag="ACHUZQ_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          142 a          136 c          129 g          127 t
ORIGIN      
        1 aaatatccct ctgatatgac aacaaaaaac ggagccggat tcctgttttt gtgaactgca
       61 gctattcgag tctaaaaagt gatccactta ccaccaacgg cctaatatat acagaacgga
      121 gtaaacaggg ctaacagttc ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc
      181 aacacaccgc cagttttagt gttgtacgca taccagagcc agtgttgttg cagccttgct
      241 gccaacgaag cgccactgct catcaagttc acagataagg gcaacatcag cgtgagcgac
      301 tggcgcagat gttatccgcc ggggcgggag ctttttaatg tccgtatgac ggtattgatg
      361 ccgattttca gtgttctggc ggtataacag ccccctgcgc cgttatgtgc catttcaacg
      421 atctgctcct taacgccggg ttttctggct tcataagagt aagtgagctg gaaaacacgt
      481 ttacatgagc gacaacggaa acgctcatgc cgggaacaac tacggccatg tcga
//
LOCUS       DAZPBM010000459          530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPBM010000459 DAZPBM010000000
VERSION     DAZPBM010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.465"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..333
                     /locus_tag="ACHUZQ_004593"
     CDS             <1..333
                     /locus_tag="ACHUZQ_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015387350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028066.1"
                     /translation="TSGSSNGSYSNLGSHRGSFTGRNTGSGTLFVYASGGNGGSAGGD
                     CANTSRLQGYVAGALISTNASNNPSYGKTAFISFAVPAGATYQITSYPAQNYSCGSGV
                     FSVFGYQT"
     gene            complement(330..>530)
                     /locus_tag="ACHUZQ_004594"
     CDS             complement(330..>530)
                     /locus_tag="ACHUZQ_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin"
                     /protein_id="HFR1028067.1"
                     /translation="ALGGKLKVTQLSSTGYLGQFDFCAIARMGNAEDSHYCQVVESPS
                     GSRKWYKYEHKTGCIASCVTLN"
BASE COUNT          135 a          125 c          122 g          148 t
ORIGIN      
        1 acttccggtt cctctaacgg tagttacagt aatcttggat cacaccgagg atcatttact
       61 ggtcgcaata cgggaagtgg tacgctgttt gtttacgcct ctggagggaa tggtggttcc
      121 gccgggggag attgtgctaa cacatccaga ttgcagggat acgttgcagg ggcattgatt
      181 agcacaaatg cgagtaataa cccatcttat gggaagacag cattcatctc ttttgctgtc
      241 cctgctggcg caacttatca gatcacctct tatccggccc aaaactactc ttgtggctca
      301 ggggtatttt cagtatttgg ataccaaact taattgagag ttacacagga cgcaatacaa
      361 cctgtcttgt gctcgtattt gtaccatttt cgtgaaccag atggactctc gactacctgg
      421 cagtaatgag aatcctcagc atttcccatt ctggcaatgg cacaaaagtc aaattgcccc
      481 aagtaaccag tgctggaaag ttgagtaacc ttgagctttc cacctaatgc
//
LOCUS       DAZPBM010000460          523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPBM010000460 DAZPBM010000000
VERSION     DAZPBM010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.467"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>523)
                     /locus_tag="ACHUZQ_004595"
     CDS             complement(<1..>523)
                     /locus_tag="ACHUZQ_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028068.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTAA"
BASE COUNT          143 a          141 c          134 g          105 t
ORIGIN      
        1 gcggcggtca tattaataat agtattcatc agatcctgca cggtattgga accgagcatc
       61 gggataatca tcagcgggat gacgatcagg aactggatcc acgctgcgcg cgccggaacg
      121 ccgttttcgt tcagttcgac ggttttctta ccaaagatgc cttccgggat ttcggagaag
      181 aaaattttca ccggcgttgc ggtccacatc agcagggaac cgaacatcgc ggtaaaggac
      241 accagaccga caaagcgatt catcaacgct tccggtagac caaaatacgc cgccatgccg
      301 tggaatacct gcaccgatcc gccggtaaat ttcaactctt tgctgctgac gaagacgtta
      361 atcagcaccg aggagacgga atacagtacg ccgataaaaa ttccggcgag gatgatcact
      421 ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acacagcgac ggactccgcg
      481 ccacctgcgg ccataaagat ccaggtggta acgccgagga acg
//
LOCUS       DAZPBM010000461          522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPBM010000461 DAZPBM010000000
VERSION     DAZPBM010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.468"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>522
                     /locus_tag="ACHUZQ_004596"
     CDS             <1..>522
                     /locus_tag="ACHUZQ_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR1028069.1"
                     /translation="IAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALI
                     SSRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGC
                     THYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAII
                     PALPKEQLLILKGS"
BASE COUNT          173 a           95 c          102 g          152 t
ORIGIN      
        1 cattgctgca caatatatta atgatattta tcagaatgtt gattacaaac cccatcaaga
       61 tgattattct tcagctgaaa aatttctcac tcacttcaac aaaaaatgca aaaaccagac
      121 tttggcgttg atttccagcc gtcctgaggg gcgttgcgtt gctgcctgcg gtgatttcgg
      181 gctagttatg aaagcatatt ttgacaagat ggaatcaaat ggcatcagtg ttatggcagc
      241 catattactg gtggataacc atgctttgac ggtccggcta agaataaaga acacaactga
      301 aggatgtacc cattacgtgg tttcggttta tgatcctaat gtaactaacg acaaaataag
      361 aatcatgagc gaaagcaaag aggatattaa acactattct ctgatggatt ttatgaatgt
      421 agattatagc ctcctgaaat ggtcaaatga tcatgttatt aaccaatctg ttgcaataat
      481 tccagcactt ccgaaagaac agctattgat attaaaagga tc
//
LOCUS       DAZPBM010000462          514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPBM010000462 DAZPBM010000000
VERSION     DAZPBM010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.469"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(116..424)
                     /locus_tag="ACHUZQ_004597"
                     /pseudo
     CDS             complement(116..424)
                     /locus_tag="ACHUZQ_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
BASE COUNT          139 a          132 c          125 g          118 t
ORIGIN      
        1 tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaatcaa
       61 catataacaa catcttaaaa aaaggcctga cattacgcca ggccttctgc gttaattaat
      121 cacgcttttc cagcagggtc cggtaaatca gaccaccgat aatgccgccg acaattggca
      181 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
      241 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actaattaag tgaatcaggg
      301 ttaaggccag accaatagcg atcggcgcaa aacctgccgg cgcgaatttg tcggttgcgc
      361 cgtggatcac caacaggaaa cctgcactca ataccagttc aactaccagc gcggaggtga
      421 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg
      481 tttctatcag ctgtccctcc tgttcagcta ctga
//
LOCUS       DAZPBM010000463          512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPBM010000463 DAZPBM010000000
VERSION     DAZPBM010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.470"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>512
                     /locus_tag="ACHUZQ_004598"
     CDS             <1..>512
                     /locus_tag="ACHUZQ_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1028070.1"
                     /translation="KVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMP
                     ADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAA
                     LWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDR
                     FGRTQTFHREA"
BASE COUNT           92 a          165 c          166 g           89 t
ORIGIN      
        1 aaggtccttc ccggtgaaac cgacatcgcc ctgcccgccc cgctgccgtt catcctctcc
       61 cgcacctaca gcagttaccg gacaaaaacg cccgcgccgg tggggagcct cggccccggc
      121 tggaaaatgc ctgcggatat ccgcttacag ctgcgcgata acacactgat actcagtgat
      181 aacggcggca gaagcctgta ttttgagcac ctgtttcccg gtgaggacgg ttacagccgc
      241 agcgaatcac tctggctggt gcgcggcggc gtggcgaaac tggatgaagg tcaccggctg
      301 gccgcactct ggcaggcgct gccggaagaa ctccgcttaa gtccgcatcg ttatctggcg
      361 acaaacagtc cgcaggggcc gtggtggctg ctcggctggt gtgagcgggt gccggaagcg
      421 gatgaggtgc tgcctgcgcc gctgccgccg taccgggtac tgaccgggct ggtggaccgc
      481 ttcgggcgca cacagacgtt ccaccgcgaa gc
//
LOCUS       DAZPBM010000464          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPBM010000464 DAZPBM010000000
VERSION     DAZPBM010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.471"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..112)
                     /locus_tag="ACHUZQ_004599"
     CDS             complement(<1..112)
                     /locus_tag="ACHUZQ_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028071.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
     gene            complement(368..>511)
                     /gene="tdcR"
                     /locus_tag="ACHUZQ_004600"
     CDS             complement(368..>511)
                     /gene="tdcR"
                     /locus_tag="ACHUZQ_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR1028072.1"
                     /translation="RYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECN
                     RFP"
BASE COUNT          155 a           99 c           97 g          160 t
ORIGIN      
        1 caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta accactgcgt
       61 gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc attgatgcct
      121 gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt tctttcgcac
      181 tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc tgtggcctac
      241 gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt atcgcggcga
      301 tcagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc tatgagggga
      361 gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa gagaaaaagt
      421 tgacgttaat caattgttcg agatgatcaa tacctgtatt cgaaatttta aaatattgat
      481 aaaataaatg tttgttctcc aacaaatagc g
//
LOCUS       DAZPBM010000465          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPBM010000465 DAZPBM010000000
VERSION     DAZPBM010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.472"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUZQ_004601"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUZQ_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1028073.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZPBM010000466          488 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPBM010000466 DAZPBM010000000
VERSION     DAZPBM010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.473"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>488
                     /locus_tag="ACHUZQ_004602"
     CDS             <1..>488
                     /locus_tag="ACHUZQ_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR1028074.1"
                     /translation="SLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAA
                     VVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACR
                     AFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAY
                     YT"
BASE COUNT           88 a          165 c          136 g           99 t
ORIGIN      
        1 ttcgctgggt gagaagaagc tggcagccca gctctgcaaa cttgcgcctc gtctgggtaa
       61 acgccttgca gcagacatag ctcaggcact ggccgaacaa accgtcgtcg ttcccggcac
      121 gaatgccgct gccgtagtac tgccacgtct ggcactccag ctcatcacgc tgcgtaagca
      181 aagagacgag gtggcgcttg aggtagaaca gcgagttctt gctcaccctc tttacccggt
      241 cctgaccagt atgcccggag tcggtgtcag gaccgcagcc agactcctca ccgaggtcgc
      301 ctgccgcgcc ttcgcctctg ccgcacatct cgctgcttat gctggccttg cgccggtaac
      361 tcggcgatcc ggctcgtcaa tacgcggtga gcatccctcg cgacggggta ataaagctct
      421 caaacgggcg ttgttcctgt cggccttcgc cgcgctcagg gatccgctct ccagggctta
      481 ctacaccc
//
LOCUS       DAZPBM010000467          486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPBM010000467 DAZPBM010000000
VERSION     DAZPBM010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.474"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>486
                     /locus_tag="ACHUZQ_004603"
     CDS             <1..>486
                     /locus_tag="ACHUZQ_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1028075.1"
                     /translation="PSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGV
                     KLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRK
                     I"
BASE COUNT          140 a          122 c          123 g          101 t
ORIGIN      
        1 cccgtcacag ccattgcaac agtcactgaa agaccttgag cgggcttaca aaaacttctt
       61 ccagaatcgg gctgcttttc cccgattcaa aaagcgggga cagaatgatg tattccgcta
      121 cccgcagggc gttaagctcg atcaggaaaa cagccgtatt tttctgccga aactgggctg
      181 gatgcgctac cggaacagcc gtcaggtcac gggtgttgtg aaaaatgtca ctgtcagcca
      241 gtcctgcggt aagtggtaca tcagtattca gacagaaagt gaagtatcaa ctccggttca
      301 cccttcagca tcaatggtcg ggctggatgc tggcgtggct aaactcgcca cgctgtcaga
      361 tggcacagtc tttgagcctg taaacagttt tcagaaaaac cagaagaagc tggcgagact
      421 tcagcgacag ttaagccgca aggtcaaatt cagcaacaac tggcagaagc agaaacgcaa
      481 aataca
//
LOCUS       DAZPBM010000468          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPBM010000468 DAZPBM010000000
VERSION     DAZPBM010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.476"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHUZQ_004604"
     rRNA            <1..>476
                     /locus_tag="ACHUZQ_004604"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZPBM010000469          469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPBM010000469 DAZPBM010000000
VERSION     DAZPBM010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.477"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>469
                     /locus_tag="ACHUZQ_004605"
     rRNA            <1..>469
                     /locus_tag="ACHUZQ_004605"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          129 a           99 c          145 g           96 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaacc
//
LOCUS       DAZPBM010000470          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPBM010000470 DAZPBM010000000
VERSION     DAZPBM010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.478"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            85..444
                     /locus_tag="ACHUZQ_004606"
                     /pseudo
     CDS             85..444
                     /locus_tag="ACHUZQ_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          113 a          110 c          137 g          108 t
ORIGIN      
        1 gtggtgatgt ctatctcaca ccgatgaaca tgaccacgaa cccctccgat ggcagtaagg
       61 ccggtaagca gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt
      121 ctgaaatctg tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag
      181 gacagccacg atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt
      241 gacagaaaag cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt
      301 tacaccgaaa tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gcttattgat
      361 gatgatcccc tcgcaaaacg tgcacatgca cacatgaagg ccggttcgtt aaccggcctt
      421 tctattgggt acgtcctgaa agactgaggt agcctgagtt taacggac
//
LOCUS       DAZPBM010000471          467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPBM010000471 DAZPBM010000000
VERSION     DAZPBM010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.479"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>467
                     /locus_tag="ACHUZQ_004607"
     CDS             <1..>467
                     /locus_tag="ACHUZQ_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1028076.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALG"
BASE COUNT          119 a          108 c          152 g           88 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cactggg
//
LOCUS       DAZPBM010000472          462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPBM010000472 DAZPBM010000000
VERSION     DAZPBM010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.480"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            163..363
                     /locus_tag="ACHUZQ_004608"
                     /pseudo
     CDS             163..363
                     /locus_tag="ACHUZQ_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          140 a          107 c          111 g          104 t
ORIGIN      
        1 actcacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc
       61 tccggcagaa gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg
      121 agttcacaga taaaacactc tccaggaaac ccggggcggt tcaaccttca cgataaaaaa
      181 cttttcatta aaggtaaagc ggatcaatat ccggcacttc attctctgat atctccgacc
      241 cgcattaatc tgaaagaaat agaaattcac tggcgtgaag tgctacgtct ggcgacctca
      301 ataaaacagg gaacagtgac agcatccctg atgctgagaa aacttgccag ttaccccaaa
      361 caaaagtaag gcagtatgac tttccgtgag acagatgctg gccaacgtgg gatatttaac
      421 gcgggttaac atcgttgtca gagccgcatg ggtgagctgt tg
//
LOCUS       DAZPBM010000473          457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPBM010000473 DAZPBM010000000
VERSION     DAZPBM010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.482"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHUZQ_004609"
     CDS             <1..118
                     /locus_tag="ACHUZQ_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1028077.1"
                     /translation="KLTKSECEKMLSGEKEAGASREQKVKLINFNYEKLQSS"
     gene            225..>457
                     /locus_tag="ACHUZQ_004610"
     CDS             225..>457
                     /locus_tag="ACHUZQ_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1028078.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          162 a           93 c           98 g          104 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgcttc
       61 cagagagcag aaagtaaaac tgataaattt taattacgag aaacttcagt cctcgtgaat
      121 ttcattatat ccagattaga acttcatagc tgattattaa aaatcaacca cacccgccag
      181 tattctgtat atttactggc ggtcatatcg tgagaggtat ggcaatgaat cttgtgacac
      241 tcaaaacgtg gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac
      301 gatgggcaag gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg
      361 tggttccgga ggctttctat atcaacccaa ataaggttga taccgatata acacaccatc
      421 agcctaatgg gcgacaaggg agagacagtc cgttaat
//
LOCUS       DAZPBM010000474          456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPBM010000474 DAZPBM010000000
VERSION     DAZPBM010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.483"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            2..388
                     /locus_tag="ACHUZQ_004611"
     CDS             2..388
                     /locus_tag="ACHUZQ_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1028079.1"
                     /translation="MKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          135 a           95 c          122 g          104 t
ORIGIN      
        1 aattaaaggg caggttgttg aacttgtacg tctgagcggt cgcgagacgt tgcggcaact
       61 ggaagccaag acaggtgcga caagatatct gatgagtgtt ctcgccagag agctggttgc
      121 cagtggcgat gtatacaact ctggttacgg gttattcccg tctgaacagg cgcgtaagga
      181 ctggcaaaac gcccgcaaaa aactctcaag ggcaaatctg aagaaaccat ctgtggttga
      241 tcctgacctt atctggtcat taccagacgg agaaatacgc cgctacgaca ggcgtcaaaa
      301 cataatctgt agcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
      361 gggtaatttt cagaaggtgc tgttgtgagc caaattaaca atcggaactt cgtgaagaga
      421 aagcataatc caaaatctga ataattaagt tcagca
//
LOCUS       DAZPBM010000475          452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPBM010000475 DAZPBM010000000
VERSION     DAZPBM010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.484"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>452)
                     /locus_tag="ACHUZQ_004612"
     CDS             complement(<1..>452)
                     /locus_tag="ACHUZQ_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1028080.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPL"
BASE COUNT           75 a          145 c          128 g          104 t
ORIGIN      
        1 caacggctgt ggccgccatg ttctgtctgc cgggattacc gcaaaatggt tctccagcgt
       61 ggtgcctgaa cccctgcgtc ccggtaccgc ctgcggctgt tcgccgtgaa gatcgctcgc
      121 caccacctgc cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg
      181 tcccggccat atctccggcg aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca
      241 gccgtccatc tgccagcggg caaagttctg cccgtgggca cccttgaaac gtccggggta
      301 gtcatacact tcatactgcg tgcgctggta atcctggtac tggccttcct gatcaaaacg
      361 ccctggccag ccgggacgtt taaaggtgta gtctttggtc actacggaag aaggacggat
      421 ttgtgcgctg tagcgaaact ggctgatgca ga
//
LOCUS       DAZPBM010000476          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPBM010000476 DAZPBM010000000
VERSION     DAZPBM010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.486"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHUZQ_004613"
     CDS             <1..>442
                     /locus_tag="ACHUZQ_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1028081.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZPBM010000477          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPBM010000477 DAZPBM010000000
VERSION     DAZPBM010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.487"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>442)
                     /locus_tag="ACHUZQ_004614"
     CDS             complement(<1..>442)
                     /locus_tag="ACHUZQ_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="endochitinase"
                     /protein_id="HFR1028082.1"
                     /translation="ALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWGFSTNCPGKAK
                     ANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQG
                     TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDA"
BASE COUNT          114 a          109 c           97 g          122 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc gg
//
LOCUS       DAZPBM010000478          439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPBM010000478 DAZPBM010000000
VERSION     DAZPBM010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.488"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            104..427
                     /locus_tag="ACHUZQ_004615"
     CDS             104..427
                     /locus_tag="ACHUZQ_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1028083.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT          163 a           67 c           72 g          137 t
ORIGIN      
        1 tttaattaat ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca
       61 tccaaatgaa ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat
      121 tgacacggta attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa
      181 aatgatcatc tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt
      241 taagcttggt attatcctta aggaaaaaga atatcctatt actatttata acattgcacc
      301 attcaacaag aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag
      361 caaaataaca aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct
      421 caagtaatgg gcttcatat
//
LOCUS       DAZPBM010000479          437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPBM010000479 DAZPBM010000000
VERSION     DAZPBM010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.489"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>437
                     /locus_tag="ACHUZQ_004616"
     CDS             <1..>437
                     /locus_tag="ACHUZQ_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1028084.1"
                     /translation="WHIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKAL
                     AEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGV
                     CGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           85 c          123 g          121 t
ORIGIN      
        1 ctggcatatt gctgccaata ttcagtttcc accaaatttt gatgagtcaa aacagtatcc
       61 ggcgattatc agcgtccatc cttttggcag ttgtaaggag cagacgtctg gcaatatcta
      121 tggcaaggca ttggctgaaa aaggctatct cgttctcgct tatgacgcca gtttccaggg
      181 agaatctggt ggggagcctc gctggataga agatccgaca cagcgcgtag aggatattag
      241 ccgtgttatc gattatgcag taactctgcc atatgttaat gctgaacgta ttggcgtgct
      301 gggagtgtgt gggggtggtg gctatgccat taatgccgcg ctgacagaaa aacgaattaa
      361 agcagtagtg agtattacgg gtgttaatat cgggcgtctt tttcgggaag gtttcagcaa
      421 ttacgatccg attggcg
//
LOCUS       DAZPBM010000480          424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPBM010000480 DAZPBM010000000
VERSION     DAZPBM010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.491"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>424
                     /gene="istB"
                     /locus_tag="ACHUZQ_004617"
     CDS             <1..>424
                     /gene="istB"
                     /locus_tag="ACHUZQ_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR1028085.1"
                     /translation="YIIHRKNLLITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRL
                     LEQLAQGRVDGSWLKYLKQLQKIQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTI
                     VVSQFPVDKWHGLMENPTTADAILDRLVHNSHRVVLQG"
BASE COUNT          131 a           92 c          118 g           83 t
ORIGIN      
        1 actacataat ccacaggaaa aacctactga taaccggtcc gacaggatgc ggtaagagct
       61 ggatagccaa tgcgctgggc gaacaggcat gtcgacagaa atacagcgtc cggtactgta
      121 gaaccgggcg attactggaa cagttggctc agggacgtgt tgatggcagt tggctgaaat
      181 atctgaaaca gttgcaaaaa atacaggttc taatcctgga cgatctgggg ctggaacagt
      241 taagtaacgc ccagtgtaat gaccttctgg agatcacaga ggatcgttac ggacaaagca
      301 gcacgatagt ggtaagccag ttcccggtag ataaatggca tggtctgatg gagaacccaa
      361 caacagcaga tgccatcctg gacagactgg tacataactc acacagagtg gtccttcagg
      421 gaga
//
LOCUS       DAZPBM010000481          424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPBM010000481 DAZPBM010000000
VERSION     DAZPBM010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.492"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            27..>424
                     /locus_tag="ACHUZQ_004618"
     CDS             27..>424
                     /locus_tag="ACHUZQ_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310463.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028086.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINENFVGMDFTCEFRPEKMTNLINIKNR"
BASE COUNT          138 a           63 c           87 g          136 t
ORIGIN      
        1 attttgttaa caatatgggg cgggagatgt tttctacaaa cttaatacag agtaattatg
       61 gtgatttaaa tattaaaagc ctggctttcg actcttttaa agaaaggctt gagtctacca
      121 tggcggcact cactttcttt atatcaacgg gtcaatgtga ctgcgatgaa atagcagagt
      181 cgaacttcaa tcgtatgatt gcctatatgt ctaacattaa ctatgatgcc tcaaagccag
      241 gagctccagc acttagtttt gatacgtatc tgcaggacaa tgttaaatat agagtgataa
      301 ttaataatct ttatggcagt gagtttcgga taagagatat taatgagaat tttgttggga
      361 tggattttac ctgtgaattc cgacccgaaa aaatgacgaa tttgatcaat ataaaaaata
      421 ggtt
//
LOCUS       DAZPBM010000482          422 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPBM010000482 DAZPBM010000000
VERSION     DAZPBM010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.493"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            100..422
                     /locus_tag="ACHUZQ_004619"
                     /pseudo
     CDS             100..422
                     /locus_tag="ACHUZQ_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           93 a          124 c          120 g           85 t
ORIGIN      
        1 tcatggcggt tatggggctt gccggtgttc tccggggtaa aaaggtccac actaccgtca
       61 gccggaaagc cgttgccgca ggcgaccgcg taaaccgtca tcaaggcaat atgccgcgta
      121 ctccgggtgg cccgcagcgg ctggtatacg tggtgtcagc ggcggacaag gataaacaca
      181 cgtcagcagt tccgtcagca ctgcgacagc gttgtccgca aggcttttac ccagtccagc
      241 gttacggtgc cccacgcctg acggatgaac tgtgtgctct ggttaccact ttaacgtaaa
      301 aaccgtggca gccagtctgc gccgtcagga actgagcgca aaagcctccc agaagttcag
      361 cccgatcagt taccgtgcac acggcctgcc tgtatcagaa aatctgctga cgcaggattt
      421 tt
//
LOCUS       DAZPBM010000483          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPBM010000483 DAZPBM010000000
VERSION     DAZPBM010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.494"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHUZQ_004620"
     tRNA            41..116
                     /locus_tag="ACHUZQ_004620"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZPBM010000484          419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPBM010000484 DAZPBM010000000
VERSION     DAZPBM010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.495"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>419)
                     /locus_tag="ACHUZQ_004621"
     CDS             complement(<1..>419)
                     /locus_tag="ACHUZQ_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1028087.1"
                     /translation="DSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPG
                     GRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESEL
                     DTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVR"
BASE COUNT           81 a          141 c          102 g           95 t
ORIGIN      
        1 cggaccggtg ctgcggcgta atacgcggca atttcaccaa tccagcccgt cagcctttcc
       61 cgctgctcct tactgttcgc gcccagaata aaatccatcg ctgactgcgt atccagctca
      121 ctttcaatgg tggcggcata catcgccttc acaatggcgc tctgcagctg cgtgttctgc
      181 agcgtgtcga gcatcttcat ctgctccatt acgctgtaaa acacatttgc accgcgggtc
      241 tgcccgtcct ccacgggttc aaaaacgtga atgaacgagg cgcgcccgcc gggtaactca
      301 cggggtatcc atgtccattt ctgcggcatc cagccaggat agccgtcctc gctgacgtaa
      361 tatcccagcg ccgcaccgct gtcattaatc tgcacaccgg cacggcagtt ccggctgtc
//
LOCUS       DAZPBM010000485          417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPBM010000485 DAZPBM010000000
VERSION     DAZPBM010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.496"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            30..206
                     /locus_tag="ACHUZQ_004622"
     CDS             30..206
                     /locus_tag="ACHUZQ_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028088.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          150 a           46 c          100 g          121 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcagg
//
LOCUS       DAZPBM010000486          411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPBM010000486 DAZPBM010000000
VERSION     DAZPBM010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.497"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>411)
                     /locus_tag="ACHUZQ_004623"
     CDS             complement(<1..>411)
                     /locus_tag="ACHUZQ_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR1028089.1"
                     /translation="RSEFDEIWKAMLTLQFHDILPGSCISRVYHETEKEYLKLEAKTE
                     KIISDAQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWLKARVNSYGYAVIVP
                     KNKIVNGLKAESQSIENNYIKLLFSESGDLISLYD"
BASE COUNT          129 a           71 c           70 g          141 t
ORIGIN      
        1 atcatatagc gaaattaaat caccgctttc tgaaaaaaga agtttaatgt aattattttc
       61 aatactttga gattctgcct tcagaccatt tacgatctta ttctttggaa caatcacagc
      121 ataaccataa gagtttactc gtgctttgag ccagttgttg tttatattga tccattcgtt
      181 acgagcaaaa caggtggtat taaacaaaat gtgcggatct ttataagaag aagtgtcaat
      241 tttacttaat aaagtagact gcgcatcaga aataattttc tcggtttttg cttctagttt
      301 aagatattcc ttttcagttt catgataaac tcttgaaata catgagccgg gaaggatgtc
      361 atggaattgc aaggtcagca tcgctttcca tatttcatca aactcactgc g
//
LOCUS       DAZPBM010000487          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPBM010000487 DAZPBM010000000
VERSION     DAZPBM010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.498"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHUZQ_004624"
     tRNA            54..130
                     /locus_tag="ACHUZQ_004624"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHUZQ_004625"
     tRNA            173..248
                     /locus_tag="ACHUZQ_004625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZPBM010000488          401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPBM010000488 DAZPBM010000000
VERSION     DAZPBM010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.499"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>401
                     /locus_tag="ACHUZQ_004626"
     CDS             <1..>401
                     /locus_tag="ACHUZQ_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1028090.1"
                     /translation="GQSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRS
                     LNDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAG
                     RINSRSTGSVEGGKSRLSVADAITRVMIVGMT"
BASE COUNT          105 a           65 c          112 g          119 t
ORIGIN      
        1 ggtcaatcta aaagtcttgg tgaatctctt ggtggcttga tgagcgatat gtcgcatggg
       61 gtcgattcac taaccgatat tagttacagg gtacgagcgt tctttgatga ggtgacatta
      121 gcttatcgtt cactaaatga cactcaaaaa gctgtagctg atggtcttgc aaatggcctg
      181 ttaagtgcat taaaggggct tgctggtata gtcgctgtac ggtctgggat tggtgctgta
      241 ggtggtatct ggaacttaat tagggccatt tccacgcttg gaaatgtggc aaatacagca
      301 gcaggaagaa tcaattcacg ttctactggt agtgttgaag gtggaaaaag taggctatct
      361 gtagctgatg caattaccag agtaatgatt gtcggtatga c
//
LOCUS       DAZPBM010000489          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPBM010000489 DAZPBM010000000
VERSION     DAZPBM010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.500"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>395
                     /locus_tag="ACHUZQ_004627"
     CDS             <1..>395
                     /locus_tag="ACHUZQ_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1028091.1"
                     /translation="LVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAGEAK
                     TDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLL
                     TQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT           76 a          137 c          100 g           82 t
ORIGIN      
        1 ctggttgttg atcagccagc taccatcggt gcgttacctg tcgccgttgc ccgctcagaa
       61 ggagtccttg tcggatacct ccctggactg gccatgcgcc gcatagccga cttacacgcc
      121 ggtgaagcta aaactgatgc tcgtgacgct gccatcattg ccgaagctgc ccgtaccctg
      181 cctcacgcgc tacgcacgct gaaactggct gacgagcaaa tcgccgaact ctccatgctc
      241 tgcggcttcg atgatgatct tgccgcacag acaacgcagg ccagcaaccg tatccgcggc
      301 cttctgaccc agatacatcc ggcactggag cgcgttctcg gtccgagact tgagcacccg
      361 gcggtactcg atcttctcca gcgatatccc tcacc
//
LOCUS       DAZPBM010000490          393 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPBM010000490 DAZPBM010000000
VERSION     DAZPBM010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.501"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..123)
                     /locus_tag="ACHUZQ_004628"
     CDS             complement(<1..123)
                     /locus_tag="ACHUZQ_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR1028092.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
BASE COUNT          150 a           86 c           59 g           98 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt gga
//
LOCUS       DAZPBM010000491          381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPBM010000491 DAZPBM010000000
VERSION     DAZPBM010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.502"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUZQ_004629"
     CDS             complement(<1..304)
                     /locus_tag="ACHUZQ_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1028093.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          117 a           75 c           80 g          109 t
ORIGIN      
        1 ttgaatcctc tctcggcacg gttgcaatta gcgagtgggt agtatcacca acagcgcgaa
       61 atccgcgtct gataatggat atgttctgat ataacgtgtt agtaggcaca cgcatacctt
      121 cttcttccca gactctggtg aaaaaatctt gttgtgtaac cacatcagga aaagcttcaa
      181 gtagaagtga taaacatcga ctggctggcg ttgtcagaac gacgttaaga tcggcgttat
      241 gtaccgatat taattttcgg tgctcaggcc agaactcgat attatcgtta ataatccagt
      301 acatacgtca ataaacacta aatcaatcga aatggagatc acatagtttg ctaagtatat
      361 tggtatttac ggcataaata t
//
LOCUS       DAZPBM010000492          380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPBM010000492 DAZPBM010000000
VERSION     DAZPBM010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.503"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..65)
                     /locus_tag="ACHUZQ_004630"
     CDS             complement(<1..65)
                     /locus_tag="ACHUZQ_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1028094.1"
                     /translation="MFDGELSFALKLAREMGRPDWR"
     gene            complement(47..>380)
                     /locus_tag="ACHUZQ_004631"
     CDS             complement(47..>380)
                     /locus_tag="ACHUZQ_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1028095.1"
                     /translation="LMKRQAEQAESDSNRKFTVEDAIRTGAFVVAMSLWHNHPKKTQM
                     PSMNEAVKQIEQEVLTTWPTEAISHAENVVYRLSGMYEFVVNDAPEQAEDAGPAEPVS
                     AGKCSTVS"
BASE COUNT           78 a          122 c           89 g           91 t
ORIGIN      
        1 cgccagtcgg gtcgccccat ctcacgcgcc agtttcaggg caaaactcag ctcaccgtcg
       61 aacactttcc cgcagaaaca ggctctgcag gcccggcgtc ctctgcctgt tcaggagcat
      121 cattcaccac aaactcatac ataccggaca gccggtacac cacgttttca gcatgagaaa
      181 ttgcctctgt gggccaggtg gtaagcactt cctgctcaat ctgtttaacg gcttcattca
      241 tggacggcat ctgcgtcttc ttcggatggt tatgccacag ggacatcgca accacaaaag
      301 cgccggttct gatggcgtct tccacagtaa acttccggtt gctgtctgac tccgcctgtt
      361 ctgcctgccg tttcatcagg
//
LOCUS       DAZPBM010000493          377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPBM010000493 DAZPBM010000000
VERSION     DAZPBM010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.504"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>377
                     /locus_tag="ACHUZQ_004632"
     CDS             <1..>377
                     /locus_tag="ACHUZQ_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1028096.1"
                     /translation="IEHKQRNTLIWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYG
                     KKHRDNTLTMKRFSNGRGFWCLGGKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTF
                     LGDKRIEGSVWPKSIRGSTPKVRG"
BASE COUNT           92 a           91 c          110 g           84 t
ORIGIN      
        1 atagagcata agcagcgcaa cacacttatc tggttgccga cggatggtga tgccgagaac
       61 tttatgaaaa cccacgttga gccgaccatc cgcgatattc cgtcgctgct ggcgctggct
      121 ccgtggtatg gcaaaaagca ccgggataac acgctcacta tgaagcgttt ttccaatggt
      181 cgtggcttct ggtgcctggg cggtaaagcg gcaaaaaact accgtgaaaa gtcggtggat
      241 gtggcgggtt atgatgaact tgctgccttt gatgaggata ttgaacagga aggctctccg
      301 acgttccttg gcgacaaacg tattgaaggc tcggtctggc caaagtccat ccgtggctcc
      361 acgcccaaag tgagagg
//
LOCUS       DAZPBM010000494          373 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPBM010000494 DAZPBM010000000
VERSION     DAZPBM010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.505"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            280..>373
                     /locus_tag="ACHUZQ_004633"
     CDS             280..>373
                     /locus_tag="ACHUZQ_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028097.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLS"
BASE COUNT          151 a           74 c           48 g          100 t
ORIGIN      
        1 attaaatcaa ttaaatcact taaacctgca ggtcatttcg taattacatt aattaaaaaa
       61 cttaaaacca ttaaataaat aaaaaaccac taactctatg tgaaataaat caaaatttca
      121 cgctgcaata ctccttagaa tgtatagcga aaggagaaaa agatatacct cgatcacccc
      181 cctttctccc aagtgaaaat aaaaggttat caacttgcaa cattgaacaa cattcgttgc
      241 aaatcgataa caacatgcac cttcaggata ctatttatta tgttcggcaa tgatattttc
      301 acccgcgtaa aacgttcaga aaataaaaaa atggcggaaa tcgcccaatt cctgcatgaa
      361 aatgatttga gcg
//
LOCUS       DAZPBM010000495          356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPBM010000495 DAZPBM010000000
VERSION     DAZPBM010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.507"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..147
                     /locus_tag="ACHUZQ_004634"
     CDS             <1..147
                     /locus_tag="ACHUZQ_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFR1028098.1"
                     /translation="KRVATVQKDGRRVEFTATSVSDLKKYIAELEVQTGMTQRRRGPA
                     GFYV"
     gene            144..>356
                     /locus_tag="ACHUZQ_004635"
     CDS             144..>356
                     /locus_tag="ACHUZQ_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1028099.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAA"
BASE COUNT           82 a           94 c          114 g           66 t
ORIGIN      
        1 aaacgggtgg caacggtaca gaaagacgga cggcgagtgg agtttacggc cacttccgtg
       61 tctgacctga aaaaatacat tgcggagctg gaggtgcaga ccggcatgac acagcgacgc
      121 aggggacctg caggatttta tgtatgaaaa cgcccaccat tcccaccctt ctgggaccgg
      181 acggcatgac atcgctgcgt gaatatgccg gttatcacgg cggtggcagc ggatttggtg
      241 ggcagttgcg ggcgtggaac ccaccgggtg aaagtgtgga tgcagccctg ctgcccaact
      301 ttacccgtgg caatgcccgc gcagacgatc tggtacgcaa taacggctat gctgcc
//
LOCUS       DAZPBM010000496          356 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPBM010000496 DAZPBM010000000
VERSION     DAZPBM010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.508"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(33..185)
                     /gene="hokA"
                     /locus_tag="ACHUZQ_004636"
     CDS             complement(33..185)
                     /gene="hokA"
                     /locus_tag="ACHUZQ_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1028100.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
BASE COUNT          104 a           81 c           69 g          102 t
ORIGIN      
        1 gaaaggcggg aataacttcc cgccaaagac tcttactctt tcaaattgca ggctaaaaat
       61 gccgccagct cataactctc ctgtttaata tgcaattcac acagtgaatc tcttatcatc
      121 caggtgaaaa ataaaagcgt gaaacaaatc actattaaag aaagtaatct atatttctgc
      181 ggcattccca gcctcctgtg ttgatttcca acgagtaaga ggctaacctt atggtgttca
      241 ggcatagggt agcctcactt tgatttatag tcaggtgggg cttttctctg tctgcctttc
      301 ggtgaatacc tgagacaaac agtctcaagc acccgtggct attctagctt aataag
//
LOCUS       DAZPBM010000497          354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPBM010000497 DAZPBM010000000
VERSION     DAZPBM010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.509"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            128..334
                     /locus_tag="ACHUZQ_004637"
     CDS             128..334
                     /locus_tag="ACHUZQ_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1028101.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          101 a           74 c           86 g           93 t
ORIGIN      
        1 caataagcaa aaataagtgg tcggatgagt agtaaaatta cacaaaaggc ggtactattt
       61 tcatcagata aacaaattca tttttccggt gctattcacc gggtagatac aaaaggggga
      121 gaaaagtatg gaactctaca gagaatatcc tgcatggctt atctttttac gccgtacata
      181 tgcggttgca gcgggcgttc tggcgctgcc tttcatgctt ttctggaaag accgcgcccg
      241 cttttacagc tacctgcacc gcgtctggtc gaaaaccagc gataaaccgg tgtggatgga
      301 tcaggccgaa aaggcaaccg gtgattttta ctgattgctg agttctgaat acat
//
LOCUS       DAZPBM010000498          352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPBM010000498 DAZPBM010000000
VERSION     DAZPBM010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.510"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..>352)
                     /locus_tag="ACHUZQ_004638"
     CDS             complement(<1..>352)
                     /locus_tag="ACHUZQ_004638"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028102.1"
                     /translation="PAAVALAEACPALVDAALADDAAAVFEPAAAEALCPAAVSEDLA
                     FVSDVLAAFAEFAAAVAEEAALLALDDAFVSDDFAASFEADASRAEVAASDAFVVAVD
                     AEVAADCCDAAAFVS"
BASE COUNT           91 a          116 c          102 g           43 t
ORIGIN      
        1 agaaacgaat gcggcagcgt cacaacaatc agcagccact tctgcatcca ccgcgaccac
       61 gaaagcgtca gaagctgcca cctcagcccg ggatgcgtcg gcttcaaaag aggcggcaaa
      121 atcatcagaa acgaacgcat catcaagcgc cagtagcgca gcttcctcgg cgacggcggc
      181 tgcaaattct gcgaaggcgg ccaaaacgtc cgagacgaac gccaggtctt ctgaaacggc
      241 agcgggacag agtgcctcag ctgcggcagg ctcaaaaaca gcggctgcgt cgtctgccag
      301 cgccgcgtca acaagtgccg ggcaggcctc agccagtgcc accgccgccg ga
//
LOCUS       DAZPBM010000499          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPBM010000499 DAZPBM010000000
VERSION     DAZPBM010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.512"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
BASE COUNT          109 a           63 c           56 g          121 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttgg
//
LOCUS       DAZPBM010000500          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPBM010000500 DAZPBM010000000
VERSION     DAZPBM010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.513"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(47..296)
                     /gene="ymgC"
                     /locus_tag="ACHUZQ_004639"
                     /pseudo
     CDS             complement(47..296)
                     /gene="ymgC"
                     /locus_tag="ACHUZQ_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          117 a           64 c           61 g          107 t
ORIGIN      
        1 ttaagtgata ctggttatct ggagattcag ggggccagtc taagttctaa gagagcacgg
       61 attccctgtc attaataatg atatccgtta gtgtagtctt atgcatttca atgtactgca
      121 atatttgcag atagagatca gaaagaatca cactggtttc atcttttaaa cgcaagcgta
      181 aagcctgatc aataatatga ctatcaacat acccatgcgt catgaacacc tccctctcga
      241 gatttttaaa caaagcacat tgaaaaaata aatctctctg ggattgaatt attcatgtca
      301 gcctcgtaga aatattaacc taataaaatt atgctgtgta tagtctaaa
//
LOCUS       DAZPBM010000501          340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPBM010000501 DAZPBM010000000
VERSION     DAZPBM010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.514"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>340
                     /locus_tag="ACHUZQ_004640"
     CDS             <1..>340
                     /locus_tag="ACHUZQ_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028103.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEG"
BASE COUNT           82 a          124 c           88 g           46 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg
//
LOCUS       DAZPBM010000502          336 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZPBM010000502 DAZPBM010000000
VERSION     DAZPBM010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.515"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(62..>336)
                     /locus_tag="ACHUZQ_004641"
     CDS             complement(62..>336)
                     /locus_tag="ACHUZQ_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR1028104.1"
                     /translation="PWGRKMHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNAL
                     FCEREIINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          105 a           64 c           47 g          120 t
ORIGIN      
        1 agggactata tatttcgttt tcagataaga ctccttgaga gattatcttt tatgtaaaat
       61 ctcaaatatc acctgatgta atgtcaatga taatattaag tgcgctctga acaacatctt
      121 tcttggtgtg attaacatct gacaaaaaga acatcagctt ctttattatg ttcttgttgt
      181 ttattatttc acgctcacag aataaagcgt tgactgcctc ttctatgaca tttctctcgt
      241 aaaaaaattg agagttagcc tcatataaaa tattggccaa tttttcacca aaaaccatat
      301 tataataatc actgtgcatc ttcctccccc agggtt
//
LOCUS       DAZPBM010000503          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZPBM010000503 DAZPBM010000000
VERSION     DAZPBM010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.516"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..332
                     /locus_tag="ACHUZQ_004642"
                     /pseudo
     CDS             1..332
                     /locus_tag="ACHUZQ_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           85 a           83 c           99 g           65 t
ORIGIN      
        1 gctatgcagc gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca
       61 tcatccggat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca
      121 gcgcgtatac gatgaaaatc acaaggtata cggtgtaaag tctggcgtca gttgttacgg
      181 gaaggtatca gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc
      241 ggtgttctcc ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc
      301 gaccgcgtaa accgtcagtt cgtggcagaa cg
//
LOCUS       DAZPBM010000504          328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZPBM010000504 DAZPBM010000000
VERSION     DAZPBM010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.517"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..203
                     /locus_tag="ACHUZQ_004643"
     CDS             <1..203
                     /locus_tag="ACHUZQ_004643"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028105.1"
                     /translation="IINTVDIHSSYPVHQKQKLTDAGLKLMATAKMPDALRLSGLRHL
                     YNILNLRAFVGRIRRSRSIRHE"
BASE COUNT           92 a           83 c           66 g           87 t
ORIGIN      
        1 ctataattaa taccgtagat attcattcat cttaccctgt gcaccaaaag caaaaactca
       61 ctgacgcggg tttgaaactg atggcaaccg caaaaatgcc tgatgcgcta cgcttatcag
      121 gcctacgcca tctctacaat atattgaatt tgcgtgcttt tgtaggcagg ataaggcgtt
      181 cacgcagcat ccggcatgaa taaagcgcac tcaacaatct gaaacccgcc ggaacggttt
      241 attacgtaca tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agtgtcgtgg
      301 acagtcattc atctttctgc ccctccaa
//
LOCUS       DAZPBM010000505          303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZPBM010000505 DAZPBM010000000
VERSION     DAZPBM010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.518"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
BASE COUNT           91 a           60 c           62 g           90 t
ORIGIN      
        1 ccgttcccag agaaataata tttattaaaa ttccagttct tctttttctg attacagaag
       61 gcaaagtggc aattacgcat agtttcccga taaagacgcg atagcgacat cccgcataag
      121 gcatttttct ctttatcttt gtacggtact tcatggaaca gagtttttga ccttgcgaat
      181 cgtgatgtct gttggggagg ggcaatttgc tcactgaagc gtgagactcg attaagcgca
      241 cgaaacacag aaatcaaaaa acccggtcac ttttttacaa ggtaaccggg taaaaataat
      301 ttt
//
LOCUS       DAZPBM010000506          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZPBM010000506 DAZPBM010000000
VERSION     DAZPBM010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.521"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUZQ_004644"
     CDS             <1..>301
                     /locus_tag="ACHUZQ_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1028106.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRAS"
BASE COUNT           76 a           56 c          100 g           69 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 g
//
LOCUS       DAZPBM010000507          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZPBM010000507 DAZPBM010000000
VERSION     DAZPBM010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.522"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUZQ_004645"
     CDS             <1..>301
                     /locus_tag="ACHUZQ_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028107.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZPBM010000508          299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZPBM010000508 DAZPBM010000000
VERSION     DAZPBM010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.524"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(178..>299)
                     /locus_tag="ACHUZQ_004646"
     CDS             complement(178..>299)
                     /locus_tag="ACHUZQ_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1028108.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT           91 a           57 c           53 g           98 t
ORIGIN      
        1 taagttaagc atcatctctc atagtgttta aattaacata tttttcaata taaaaaataa
       61 gactctggct tcaattgtgc gcggattttc ttacaggtgt aggataatag ccttcacaaa
      121 ataattatct gcgcaatttt cgtgaatgga gcgcatagac taaggtcaaa atcacaatta
      181 ttcatagata aaagtgacac caatgactga ctggacggtt ccggctgaaa ccttgtttcc
      241 tgtttgtcca taggttgcgc ttaaacccag aggtactttg cttttattta ctgtcccca
//
LOCUS       DAZPBM010000509          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZPBM010000509 DAZPBM010000000
VERSION     DAZPBM010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.525"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHUZQ_004647"
     CDS             <1..279
                     /locus_tag="ACHUZQ_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1028109.1"
                     /translation="LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGA
                     LLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT           70 a           77 c           89 g           59 t
ORIGIN      
        1 ctgacgtact atgcagatga tggctgggct gaggcggaca ataacatcgc tgaaaatgcg
       61 ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct tcggttcgga tcatggagga
      121 gagcggggag cgctgctgta cagcctgatc gggacgtgca aactgaacgg agtggagcca
      181 gaaagctacc tccgctatgt ccttgacgtc atagccgact ggccgataaa ccgggtcggc
      241 gaactgctcc cctggcgcgt agcactgccg actgaataac acattcccgt caata
//
LOCUS       DAZPBM010000510          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZPBM010000510 DAZPBM010000000
VERSION     DAZPBM010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.526"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(<1..263)
                     /locus_tag="ACHUZQ_004648"
     CDS             complement(<1..263)
                     /locus_tag="ACHUZQ_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR1028110.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSL"
BASE COUNT           83 a           66 c           68 g           75 t
ORIGIN      
        1 ataagtgagg gggcgcagac catgcagtca aaaccggatt tcaccagccg acgataaagt
       61 ccataaccac agggccccgc ttcataaacg atactgacct gcgatgactt tgacctgaga
      121 cgtttacaaa gattctgaat atcagtaggt gatgtgccaa ttttacccat cagttcaaca
      181 ggttctgaat tgatagcata agcgacagta atcgattctt tgtgaacatc taacccgacg
      241 tacaatgtgt tattgttgtt catgctggcc tccgtggtgg aaataccggg gt
//
LOCUS       DAZPBM010000511          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZPBM010000511 DAZPBM010000000
VERSION     DAZPBM010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.527"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHUZQ_004649"
     CDS             <1..>292
                     /locus_tag="ACHUZQ_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1028111.1"
                     /translation="RGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNG
                     GDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           80 g           78 t
ORIGIN      
        1 atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg gtttgtgcgg
       61 taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag atgtctttgt
      121 ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag aaacaagcga
      181 aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac agtcgtaccg
      241 gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg ca
//
LOCUS       DAZPBM010000512          291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZPBM010000512 DAZPBM010000000
VERSION     DAZPBM010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.528"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>291
                     /locus_tag="ACHUZQ_004650"
     CDS             <1..>291
                     /locus_tag="ACHUZQ_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR1028112.1"
                     /translation="QGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKN
                     EWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSN"
BASE COUNT           87 a           66 c           80 g           58 t
ORIGIN      
        1 atcagggcag tcattatacg agcaggcagt tccggcagtt attgtggcga taccagatca
       61 gacagagtat gagccggcgc ggaaactgct gggataacag cccaatggaa cgcttcttca
      121 ggagtctgaa gaacgaatgg atgccggtgg tgggttacgt aagcttcagc gaggcagctc
      181 acgccataac ggactatatc gttggatatt acagcgcact aagaccgcac gaatataacg
      241 gtgggttacc cccaaacgaa tcggaaaatc gatactggaa aaactctaac t
//
LOCUS       DAZPBM010000513          286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZPBM010000513 DAZPBM010000000
VERSION     DAZPBM010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.529"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
BASE COUNT           97 a           67 c           58 g           64 t
ORIGIN      
        1 ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt tcccggggtt tgcttagaat
       61 attttgtaag ttgtccgaag agatatttac aacaccagaa tgatgaatca tcagtcccct
      121 gccagaggtt cactacgtaa cctgaccgac aatgcaccat tctggtgctg aaaaaagagc
      181 actggaactg caacaaaaca acatcacaga acaggagatg gtaaggagtc agacattccc
      241 acacaatatt gtgtcagtgc aaataacaac cttcgtctca aatctc
//
LOCUS       DAZPBM010000514          284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZPBM010000514 DAZPBM010000000
VERSION     DAZPBM010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.530"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>284
                     /locus_tag="ACHUZQ_004651"
     CDS             <1..>284
                     /locus_tag="ACHUZQ_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1028113.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGA"
BASE COUNT           72 a           55 c           88 g           69 t
ORIGIN      
        1 gcgacttatc agtggaaaaa cacagaacga tcaggatata tgcgccctgg caaaagagta
       61 tgagatcgcc atgctgttgg tgactaaagg tgcagaaggg gtggtggtct gttatcgagg
      121 acaagttcac cattttgctg gaatgtctgt gaattgtgtc gatagcacgg gggcgggaga
      181 tgcgttcgtt gccgggttac tcacaggtct gtcctctacg ggattatcta cagatgagag
      241 agaaatgcga cgaattatcg atctcgctca acgttgcgga gcgc
//
LOCUS       DAZPBM010000515          284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZPBM010000515 DAZPBM010000000
VERSION     DAZPBM010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.531"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(201..>284)
                     /locus_tag="ACHUZQ_004652"
     CDS             complement(201..>284)
                     /locus_tag="ACHUZQ_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028114.1"
                     /translation="AGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT           86 a           68 c           38 g           92 t
ORIGIN      
        1 taacaatcgc actccatttt ctctttttaa aaaacataaa cactgttaaa acaaatactt
       61 atttcgttat cttaaatgct atttctacat cactcgtcct tataatcaaa ttattcttat
      121 tcatagctat ttttgcaggt ttaaaatagc ggctttcacc aaagtcagcc atgaaaatta
      181 gactacaagt cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag
      241 tttctgtcca tggctgcttt tcgcatctta cgtcttaacc ctgc
//
LOCUS       DAZPBM010000516          277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZPBM010000516 DAZPBM010000000
VERSION     DAZPBM010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.533"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>277
                     /locus_tag="ACHUZQ_004653"
     CDS             <1..>277
                     /locus_tag="ACHUZQ_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1028115.1"
                     /translation="KAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPGDGRTLVVHFD
                     KFGNVEFTETVPDGALVCPLETFRFYLEKQGWTLVNRGAIKNMTVEQ"
BASE COUNT           74 a           69 c           69 g           65 t
ORIGIN      
        1 aaaaagccgc cgcattcaaa gaggcataca ttgctgagtt cgaccgcatg gaggccgaac
       61 tgcgccagaa taacacccca cctgctgaca agatgattcc gggtgatggt cgcactctgg
      121 ttgttcactt cgacaaattc ggcaatgtcg aattcaccga aaccgttcct gatggcgcac
      181 tggtctgtcc cctggagact ttccggtttt atctggaaaa acagggatgg actcttgtta
      241 accggggcgc aattaaaaat atgactgtgg aacaatt
//
LOCUS       DAZPBM010000517          274 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZPBM010000517 DAZPBM010000000
VERSION     DAZPBM010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.534"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>274
                     /locus_tag="ACHUZQ_004654"
     CDS             <1..>274
                     /locus_tag="ACHUZQ_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1028116.1"
                     /translation="SNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETATELV
                     EDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDF"
BASE COUNT           59 a           68 c           93 g           54 t
ORIGIN      
        1 cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc tacagtttca gcgccctgaa
       61 agaccgccat aatgccgttg aggtgaactg gattgacccg gataacggct gggagacggc
      121 gacagagctt gtggaggata cgcaggccat tgtccgttac ggtcgtaacg tcacgaagat
      181 ggatgccttt ggctgtacca gccgggggca ggcacaccgc gccgggctgt ggctgattaa
      241 aacggaactg ctggagacgc agaccgtgga tttc
//
LOCUS       DAZPBM010000518          272 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZPBM010000518 DAZPBM010000000
VERSION     DAZPBM010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.535"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..>272
                     /locus_tag="ACHUZQ_004655"
     CDS             <1..>272
                     /locus_tag="ACHUZQ_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028117.1"
                     /translation="ITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLR
                     LQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
BASE COUNT           91 a           71 c           52 g           58 t
ORIGIN      
        1 ccataacaga ggccaccctg tacgaaaacg ggctgtatac gcgccacatc agactcagtc
       61 gcccagtatc tgacagcaaa taccccttgc cacccggatc tcagctcatt aaaaatatga
      121 ccttacgact gcgcctgcag gatcagcagg aagaaacatc cgtgacatta tttcagggaa
      181 aaatgagtac cgacggaaat aactattaca tcatcaaagg taaagtaagc tcagtacttc
      241 tgttattaaa aatgctgcag attaatcacg cc
//
LOCUS       DAZPBM010000519          271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZPBM010000519 DAZPBM010000000
VERSION     DAZPBM010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.536"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..183
                     /locus_tag="ACHUZQ_004656"
     CDS             <1..183
                     /locus_tag="ACHUZQ_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFR1028118.1"
                     /translation="NETGEDELNWKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIAN
                     KLENFGVTSDERLPDF"
     gene            161..>271
                     /locus_tag="ACHUZQ_004657"
     CDS             161..>271
                     /locus_tag="ACHUZQ_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1028119.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEK"
BASE COUNT           82 a           55 c           72 g           62 t
ORIGIN      
        1 aatgaaactg gcgaagatga actcaactgg aaaaaacagg tgttgattgc ggcggtttac
       61 ggtttatgtg cgaatcctgc gtgcatagcc tatgcgcctg caatccctga catcgcaatg
      121 atgattgcca acaagcttga aaattttgga gtgaccagtg atgagcgctt acccgatttt
      181 tgatcgtatt gaagaaatgg catgggctcg ccactaccag cagatcgttc gtgaagaaaa
      241 agaaacggaa ctggcggacg acctggaaaa a
//
LOCUS       DAZPBM010000520          259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZPBM010000520 DAZPBM010000000
VERSION     DAZPBM010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.537"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            2..>259
                     /gene="istB"
                     /locus_tag="ACHUZQ_004658"
     CDS             2..>259
                     /gene="istB"
                     /locus_tag="ACHUZQ_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR1028120.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQ"
BASE COUNT           74 a           61 c           76 g           48 t
ORIGIN      
        1 tatgagtgat aatctgttaa acaaactgac ccaactgaaa ctcccggcga tggccgggag
       61 cctgatacgg caacgggaaa cgcctcagac ttacgacgaa ctgtcgttcg aggaaaggct
      121 gacactgctg gttgatgacg agcttctgag cagagaaaac agtcgtgtgg cacggttgag
      181 aaaaaatgcc tgcctgaaat atcaggcaac accggaaggg ctgcgttatc cggcctcaag
      241 aggtctgcgg gctgaacag
//
LOCUS       DAZPBM010000521          258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZPBM010000521 DAZPBM010000000
VERSION     DAZPBM010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.538"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHUZQ_004659"
     CDS             <1..161
                     /locus_tag="ACHUZQ_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1028121.1"
                     /translation="VKLSMPELEELAAAMVQAVVERNDEIYRRQREMKEELSSLDDLA
                     SIRAFDVK"
BASE COUNT           64 a           53 c           86 g           55 t
ORIGIN      
        1 aggtgaaact gtcgatgccg gaactggaag aactggcggc agcaatggtg caggctgttg
       61 ttgaacgcaa tgacgagatt tatcgccgtc agcgtgaaat gaaagaggag ctgagcagtc
      121 tggatgattt ggcttcaatt cgggcgtttg acgttaagta atgaataagc cgcaactggc
      181 ggaatcacag aagaccgctt tgcttaccga ggcggagtct gtcatccggc cgccggggtg
      241 tgcggtcagg ctgaacag
//
LOCUS       DAZPBM010000522          257 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZPBM010000522 DAZPBM010000000
VERSION     DAZPBM010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.denovo.539"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..257
                     /locus_tag="ACHUZQ_004660"
                     /pseudo
     CDS             1..257
                     /locus_tag="ACHUZQ_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    <1..115
                     /locus_tag="ACHUZQ_004660"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT           70 a           60 c           71 g           56 t
ORIGIN      
        1 cttattttgc aaaggcggag ttcgaccgcc tctggaaaaa ataatgccac tgctggataa
       61 gctgcgtgag cagtacgggg tcggatcggt atgcagtgaa ctgcatattg ccccgtcaac
      121 gtattaccac tgtcagcaac agcgacatca tcatgataaa cgcagtgccc gtgcgcagcg
      181 cgatgactgg ctgaagaaag agatactgcg cgtatacgat gagaatcatc aggtatacgc
      241 tgtgcgtaaa gtctggc
//
LOCUS       DAZPBM010000523        13061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.guided.116, whole genome shotgun sequence.
ACCESSION   DAZPBM010000523 DAZPBM010000000
VERSION     DAZPBM010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.guided.116"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHUZQ_004661"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHUZQ_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUZQ_004662"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUZQ_004662"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1028122.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUZQ_004663"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUZQ_004663"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1028123.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUZQ_004664"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUZQ_004664"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1028124.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUZQ_004665"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUZQ_004665"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1028125.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUZQ_004666"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUZQ_004666"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1028126.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHUZQ_004667"
     CDS             5099..6232
                     /locus_tag="ACHUZQ_004667"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1028127.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHUZQ_004668"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHUZQ_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1028128.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUZQ_004669"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHUZQ_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1028129.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHUZQ_004670"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHUZQ_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1028130.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHUZQ_004671"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHUZQ_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1028131.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHUZQ_004672"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHUZQ_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1028132.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="ACHUZQ_004673"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="ACHUZQ_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1028133.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9803..10672
                     /locus_tag="ACHUZQ_004674"
     CDS             9803..10672
                     /locus_tag="ACHUZQ_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1028134.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10875..11228)
                     /locus_tag="ACHUZQ_004675"
     CDS             complement(10875..11228)
                     /locus_tag="ACHUZQ_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1028135.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11366..13012)
                     /gene="groL"
                     /locus_tag="ACHUZQ_004676"
     CDS             complement(11366..13012)
                     /gene="groL"
                     /locus_tag="ACHUZQ_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1028136.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
BASE COUNT         3277 a         3385 c         3258 g         3141 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     9541 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
     9601 ttgcataacc tttaattatt catatacatt gctgttgtta ctctttatat agccaacacc
     9661 aataattaac ctttcttttc tgtttatata gagtgagtac ggctatttat gttattttaa
     9721 caactattac taccaagccc atttatctgg cttataaatt aaaattaatt atttaattga
     9781 gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata ccggtgttat
     9841 tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc gcaataaaga
     9901 atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc tggaaagtcg
     9961 tctgcatcaa aagcataaac tgaatgctgc tgcacgcctt cagtatgagc aagctcgcga
    10021 taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg agcttaaaaa
    10081 tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag gcgaaaatct
    10141 taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca atgagttgca
    10201 gattgaaatg ctggcgcgag caatcattca tgcaattaat aatgcggaaa tgcgtgaact
    10261 ggcattacgt attacctcac tgttagattt cctgccgtta tacgacgttg actgccagga
    10321 caatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata acctgttcga
    10381 ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac aatttagcgg
    10441 cacagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa atagaggcga tcactcgccg
    10501 aatgcttgat ttcagcccac gactgaaaaa actcgcgggt gtgccatgtc aggtctatgt
    10561 tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct tacgcgcgct
    10621 gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat aatctcatct
    10681 ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga tcgttggtca
    10741 tgccggatgc gacgtaaacg ccttatccgg cctacaaatt ctcaagcatt caatcagttg
    10801 caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc aataatacgt ttgtcaccca
    10861 gcctctggat cacatcagtt cgggcactta taaacctgac cgataatctg gctatccgtc
    10921 gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat cacgttaccg
    10981 cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga accgccctct
    11041 tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc aatcagctgg
    11101 cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc actcaattcg
    11161 ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc taagtatttt
    11221 acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt ccgtgtctga
    11281 atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc cgggggtttg
    11341 tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc atgccgccca
    11401 taccgccagc agcgcctaag tcagctgcat catttttcgg caggtcggta accatgcact
    11461 cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa cgggttactt
    11521 tggttgggtc caggataccc atgtcgatca tgttgccgta ttcttcggtt gctgcgttgt
    11581 aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac aacagacggt tcttcgccgc
    11641 agttcaggac gatctgacgc agcggagctt ccattgcacg cagtgcaact ttgataccca
    11701 cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg cggatcagcg
    11761 caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg tgcagggcgt
    11821 cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct actttgataa
    11881 ctgcaacgcc gcctgccagt ttcgctacgc gttcctgcag tttttcacgg tcgtagtcag
    11941 aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt gcagcttctt
    12001 cacccacgcc atcgatgata gtggtggtgt ctttgttgat cacaacacgt ttagcctgac
    12061 ccaggtcttc cagggttgct ttttccagtt ccataccgat ctcttcagag atcacggtac
    12121 cgccagtcag ggttgcgata tcctgcagca tagctttacg acgatcgccg aagcccggag
    12181 ctttaactgc agcaactttc acgatgccac gcatggtgtt aacaaccaga gttgccagcg
    12241 cttcgccttc tacatcttca gcgatgatca gcagcggttt gcctgctttg gcaacggctt
    12301 ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc aggatgaacg
    12361 ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga gacaggtagc
    12421 cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg gtaccgtctt
    12481 caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc agtttaccta
    12541 cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta gagtcagagc
    12601 acggtacgga cagcgctttc agttcttcaa ctgcagcggt aacggctttg tcgataccac
    12661 gtttcaggtc catcgggttc atgcccgcag caacagcttt cagaccctca gtgatgatag
    12721 cctgagccag tacagttgca gtggtggtac cgtcgcctgc agcgtcgttc gctttagagg
    12781 caacttcttt caccatctgc gcacccatgt tttcgaactt gtcttccagt tcgatttcac
    12841 gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta tccagaacta
    12901 cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg tttacgccgc
    12961 gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc attatcttta
    13021 ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg c
//
LOCUS       DAZPBM010000524         6065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.guided.215, whole genome shotgun sequence.
ACCESSION   DAZPBM010000524 DAZPBM010000000
VERSION     DAZPBM010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.guided.215"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            <1..816
                     /gene="sul2"
                     /locus_tag="ACHUZQ_004677"
     CDS             <1..816
                     /gene="sul2"
                     /locus_tag="ACHUZQ_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR1028137.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            903..1205
                     /locus_tag="ACHUZQ_004678"
     CDS             903..1205
                     /locus_tag="ACHUZQ_004678"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028138.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1381..2874)
                     /locus_tag="ACHUZQ_004679"
     CDS             complement(1381..2874)
                     /locus_tag="ACHUZQ_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFR1028139.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(2918..3307)
                     /locus_tag="ACHUZQ_004680"
     CDS             complement(2918..3307)
                     /locus_tag="ACHUZQ_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028140.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            3525..3965
                     /locus_tag="ACHUZQ_004681"
     CDS             3525..3965
                     /locus_tag="ACHUZQ_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR1028141.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(4688..4837)
                     /locus_tag="ACHUZQ_004682"
     CDS             complement(4688..4837)
                     /locus_tag="ACHUZQ_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1028142.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            5408..5566
                     /locus_tag="ACHUZQ_004683"
     CDS             5408..5566
                     /locus_tag="ACHUZQ_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR1028143.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
BASE COUNT         1442 a         1672 c         1586 g         1365 t
ORIGIN      
        1 atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag tttctccgat
       61 ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct gatggccgag
      121 ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc gcctgtttcg
      181 tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc agatggcatt
      241 cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg
      301 gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca attggcgaaa
      361 tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag
      421 gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg
      481 ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat ggggtttttt
      541 ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga attgcggctg
      601 cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca
      661 ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc cgccgccgca
      721 ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg gctggcggta
      781 ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga tatttctcta
      841 tattcgcggt tcagcaggca tgtccccttt gagggcgacc cgacgacagg ataatcgacc
      901 ttatggtgcg caaatatttc ggcacagacg gtattcgtgg caaagccaac gaaggcgcga
      961 tgacggcgga aaccgccttg cgcgtcggca tggcggctgg ccgtgtcttt cgtcgcggtg
     1021 accaccgcca tcgtgtcgtg atcggcaagg atacgcgcct gtcgggctat atgcttgaac
     1081 ccgcgctcac agccggtttc acctcgatgg gcatggacgt attccttttt ggcccgctgc
     1141 cgacaacgta taggaagaat aaacgccctt ttcacccaag tccaacagct ttggaccgca
     1201 gttgactctt tcgacacccc tgcgatgcaa cccaatccgg ctgacgggga gccagcaacg
     1261 ctgaaaattt accctcctct ttcccactag cggctccttt tccgacaacc agcacggcgg
     1321 atccctgccg cggcgctgtg aacgcagcat tttgattggt atcgttggcc ttcaggctcg
     1381 tcagtcaaac agacccagga gcagctcagc cggtggcgcc cggctttcgg gtaacgccct
     1441 ggtcccgctg gtttcggctt tgtgcttcag gtgatcaagg atctgcttga tcactatagg
     1501 gtcttcaatg caggcgatga ctttcatggc gccgccgcag ccgctgcagg tctcgatgtc
     1561 gatattgaaa acacgcttga gccgttgcgc ccatgtcatc gacgctcgcc gttgtgctgg
     1621 tgttgccggt tcatcagcca ccctgacctt gttgcccctg ccccgttttg ccggcgtgac
     1681 caacgcccgg tgccgactgt tgggtgcgaa caccccgtgg aagcgggtta ggttgactct
     1741 gggcttcggt accagggcgg ccagccttgc aatgaaatcc aatggttcga aaatgacgtg
     1801 cgtggtgccg tcccggtacg gcgtcttgag ctggtagcgc acgttgccgc ctcgtgttaa
     1861 cgacagccgc ttctcggata ccgccgggcg gctgatgtac cggcacagcc gttcgagctt
     1921 cttgcgttca tcggccctgg ccgccacgcc ggcgtgcagg ctggacccgg ctaccttgcc
     1981 aatcccgtca ccgaacggat caccactggt cggcagagtt tgcaaagtga acacctttcg
     2041 ccccgcctgt gaaccgacag cgatacggta agtgatcgag tgccccagca ggggtgtcat
     2101 cgggtcgtca tccaccgcat ccgaggccag atagctgttt tcgacatccc gttccagcag
     2161 gccttgccgt tccagatagc gacccacccg gtgggcgatg gtgtgcgtca gctgggtgag
     2221 ctctgggctg gtcggcgcct tgacccagcg gaaacgcgct gagccgtggg attgctcgac
     2281 atacacaccg tcgagaaaca gcatgtggaa gtgaacattc agattgagcg ccgatccaaa
     2341 acgctggatc agggtgaccg cgcccgtctt ggccacttgg tgggtatggc ccgctttctt
     2401 gaccaggtgc gtggcaatga cgcggtaaac gatgcccagc acccacccca tgatctcggg
     2461 ccggctggca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     2521 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     2581 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     2641 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     2701 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     2761 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     2821 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     2881 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     2941 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     3001 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     3061 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     3121 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     3181 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     3241 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     3301 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     3361 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     3421 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     3481 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     3541 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     3601 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     3661 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     3721 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     3781 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     3841 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     3901 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     3961 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     4021 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     4081 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     4141 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     4201 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     4261 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     4321 acgtatgagc tgaaggaatg atgtaaaagt gatgtatgaa ctgaaggagc gatgtaattc
     4381 tgtagtcagg aagaataacc cgttcatgca ggcatgaacg atacagtgag gaacgctttt
     4441 tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt atttttcaac
     4501 ctcaaaatgg tcatacacct aacgcagccg cgattttcat cgcaactgca tgaattaaaa
     4561 gaaaaaaaag gaatatttcg ttttcacgag taatacccgg gtattaaaaa tccactgtaa
     4621 ttacatgaaa ataaagaaat aaaaggtaat accggagtat taccacgtac gcgggcaagc
     4681 gaaaaagcta taacgcttcc cggccgacag gcgacccaaa atccacaggg gcatgcaggt
     4741 tctcttcagt aacctgcgcc attaaatcaa aaaggtcata ttcgccggga agctgcgtat
     4801 cagtcgaatc cagttgttca tcatattcag atggcatggg gtgatatcca gtagctgaga
     4861 ggttgttagc gggtttcggg gcgcagccct gaaccagtca tgtagcgcta gcggagtgta
     4921 tactggctta ctatgttgcc acgattgcga aaatcagtga agtgcttcat gtggcaggag
     4981 aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac aggagatatt ccgcttcctc
     5041 gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg cttacggacg
     5101 gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag ataaggccac
     5161 ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa tctgacgctc
     5221 aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc cccctggtag
     5281 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     5341 gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc gctccaagct
     5401 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg gtaactatca
     5461 acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca ttagtaacag
     5521 gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc ggctacactg
     5581 aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa gcagttcccc
     5641 aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt acagagcaag
     5701 agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact atcaattaat
     5761 cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta acaggtttcc
     5821 ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc tacttattga
     5881 gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg gatcaccgca
     5941 aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag actcatgctc
     6001 gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg gcatcgtcaa
     6061 cataa
//
LOCUS       DAZPBM010000525         4819 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2007629
            SAMN43277556-rid23489073.guided.244, whole genome shotgun sequence.
ACCESSION   DAZPBM010000525 DAZPBM010000000
VERSION     DAZPBM010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277556
            Sequence Read Archive: SRR30464349
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396700.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396700.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:37:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,688
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,327
            CDSs (with protein)               :: 4,327
            Genes (RNA)                       :: 87
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 274
            CDSs (without protein)            :: 274
            Pseudo Genes (ambiguous residues) :: 0 of 274
            Pseudo Genes (frameshifted)       :: 124 of 274
            Pseudo Genes (incomplete)         :: 93 of 274
            Pseudo Genes (internal stop)      :: 88 of 274
            Pseudo Genes (multiple problems)  :: 30 of 274
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277556-rid23489073.guided.244"
                     /strain="M2007629"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-09-18"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHUZQ_004684"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHUZQ_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHUZQ_004685"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHUZQ_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1028144.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUZQ_004686"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUZQ_004686"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1028145.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUZQ_004687"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUZQ_004687"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1028146.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..>4819
                     /gene="frsA"
                     /locus_tag="ACHUZQ_004688"
     CDS             3588..>4819
                     /gene="frsA"
                     /locus_tag="ACHUZQ_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1028147.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIE"
BASE COUNT         1145 a         1207 c         1289 g         1178 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaa
//
